[Effect of carminomycin on the structural integrity, primary susceptibility to radiation damage and the postradiation repair of the cellular DNA-membrane complex in Ehrlich's ascitic carcinoma].
It was found with the method of sedimentation analysis in sucrose alkaline gradients that carminomycin induced in vivo degradation of the DNA-membrane complex of the Ehrlich's ascites carcinoma cells (EAC). The concentration dependence of the complex damage correlated well with the data on the cell survival: the subtoxic concentration of the antibiotic according to these tests was 0.001 micrograms/ml. Combined effect of carminomycin in the subtoxic concentration and gamma-radiation on the cells did not result in any increase in the primary radiation damage to the DNA-membrane complex. Still, it inhibited its post-radiation reparation. This suggested that on the combined use of the above damaging agents the antibiotic significantly increased the effect of the ionizing radiation on the cells.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy*; D002360:Carubicin / Q000627:therapeutic use*; D002462:Cell Membrane / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D004260:DNA Repair / Q000528:radiation effects*; D004273:DNA, Neoplasm / Q000528:radiation effects*; D003630:Daunorubicin / Q000031:analogs & derivatives*; D005720:Gamma Rays; D051379:Mice; D009368:Neoplasm Transplantation
D004273:DNA, Neoplasm; D002360:Carubicin; D003630:Daunorubicin
Deĭstvie karminomitsina na strukturnuiu tselostnost', pervichnuiu radioporazhaemost' i postradiatsionnuiu reparatsiiu DNK-membrannogo kompleksa kletok astsitnoĭ kartsinomy Erlikha.