Association of phycoerythrin and phycocyanin: in vitro formation of a functional energy transferring phycobilisome complex of Porphyridium sordidum.
Functional in vitro association and dissociation of a phycobiliprotein complex, isolated from phycobilisomes of the red alga Porphyridium sordidum, were studied. The complex contained large bangiophyceaen phycoerythrin and cyanophytan phycocyanin in an equimolar ration and had absorption maxima at 625, 567, and 550 nm and a shoulder at 495 nm. Emission at 655 nm (with excitation at 545 nm) from phycocyanin indicated functional coupling. The complex was stable over a wide buffer concentration range, and, notably, it was maximally stable in low phosphate, less than 0.01 M, unlike the phycobilisomes, which dissociate at this concentration. Its molecular weight was estimated to be ca. 510000, and by electron microscopy it was seen to consist of two units of similar size. The complex in 0.1 M phosphate was separated on a sucrose gradient into a homogeneous phycoerythrin band and a spectrally heterogeneous phycocyanin band. In vitro association of phycoerythrin and phycocyanin resulted in a complex with the same absorbance, emission, sedimentation and molar pigment ratio as those of the native complex. The spectrally heterogeneous phycocyanin fractions from the dissociation gradient varied in the degree of association with phycoerythrin. Phycocyanin fractions absorbing from 622 to 633 nm exhibited high associability (greater than 70%), whereas those with maxima at 617-620 nm had low associability (less than 30%). The presence of a 30000 molecular weight polypeptide accompanied high associability, where it was ca. 2-fold more prominent. It is suggested that this polypeptide is involved in complex formation and could serve either in the stabilization of the conformational state of cyanophytan phycocyanin or as a direct linker between phycobiliproteins.
D004735:Energy Transfer; D007700:Kinetics; D008854:Microscopy, Electron; D008970:Molecular Weight; D045524:Phycobilisomes; D010798:Phycocyanin / Q000302:isolation & purification / Q000378:metabolism*; D010799:Phycoerythrin / Q000302:isolation & purification / Q000378:metabolism*; D010860:Pigments, Biological / Q000378:metabolism*; D000461:Rhodophyta / Q000378:metabolism* / Q000648:ultrastructure; D013053:Spectrophotometry
D045524:Phycobilisomes; D010860:Pigments, Biological; D010798:Phycocyanin; D010799:Phycoerythrin