A spectroscopic analysis of the thermally induced folding-unfolding transition of beta-trypsin.
Absorption and fluorescence changes were used to monitor the thermally induced folding-unfolding transition of beta-trypsin. These parameters reflect changes in the microenvironment of different subsets of the four tryptophanyl residues of this protein. The thermal transition was found to be sequential.
D000042:Absorption; D005453:Fluorescence; D008968:Molecular Conformation; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology*
D014357:Trypsin