title: Membrane-active agents. Effect of various anesthetics and chlorpromazine on arterial lipid metabolism.
abstract: The local anesthetic lidocaine was studied for its effects on lipid metabolism in aortas from normal rats, rabbits, and cholesterol-fed (atherosclerotic) rabbits in vitro. Incubation of aortas in the presence of 3--5 mM lidocaine resulted in a statistically significant reduction in the incorporation of [14C]oleate into cholesteryl esters and phosphatidylcholine. Additionally, significant increases in [14C]oleate incorporation into the diglyceride fraction of atheromatous rabbit aortas was observed with a trend to greater incorporation into the diglyceride fraction of normal rat and rabbit arteries as well. The most significant overall effect of lidocaine was its inhibition (50--90%) of the arterial sterol esterification. Assays of acylCoA : cholesterol acyltransferase (ACAT, EC 2.3.1.26) in isolated arterial microsomes revealed that, in addition to local anesthetics (e.g., lidocaine), other membrane-active agents such as chlorpromazine and methoxyflurane inhibit ACAT; this suggests ACAT may be regulated by alterations in the biophysical properties of its membrane milieu.
mesh_terms: D000102:Acetyl-CoA C-Acyltransferase / Q000037:antagonists & inhibitors; D000779:Anesthetics, Local / Q000494:pharmacology*; D000818:Animals; D001158:Arteries / Q000187:drug effects / Q000378:metabolism*; D002746:Chlorpromazine / Q000494:pharmacology*; D008012:Lidocaine / Q000494:pharmacology; D050356:Lipid Metabolism; D008297:Male; D008563:Membrane Lipids / Q000378:metabolism; D009829:Oleic Acids / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011817:Rabbits
chemical_list: D000779:Anesthetics, Local; D008563:Membrane Lipids; D009829:Oleic Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D008012:Lidocaine; D000102:Acetyl-CoA C-Acyltransferase; D002746:Chlorpromazine