An investigation of the effect of radioactive labeling of DNA on excision repair in UV-irradiated human fibroblasts.
Previous studies on the kinetics of thymine dimer excision and unscheduled DNA synthesis in UV-irradiated human fibroblasts showed a significant discrepancy in these two parameters (Ehmann et al., 1978. Biophys. J. 22: 249). In the present study we have investigated the effect of the level of the radioactive isotope used for labeling cells on the kinetics of a parameter that directly measures thymine dimer excision. We find no significant differences in the kinetics of this parameter in cells lightly or heavily labeled with radioactive thymidine.
D002250:Carbon Radioisotopes; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair / Q000528:radiation effects*; D004171:Diploidy; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D011740:Pyrimidine Dimers / Q000378:metabolism*; D014316:Tritium; D014466:Ultraviolet Rays
D002250:Carbon Radioisotopes; D011740:Pyrimidine Dimers; D014316:Tritium; D004247:DNA