Allergenic activity of fractions of cocksfoot (Dactylis glomerata) pollen. Definition of active components by skin testing, and inhibition RAST.
With the combined use of skin tests, RAST and isoelectric focusing, a potency profile of a cocksfoot ((Dactylis glomerata) pollen extract was prepared. It was found that two fractions with pI 4.5 and 6.0 were the most allergenic, had the highest protein concentration and were also the most easily extracted. The importance of converting mean weal sizes into potency units when comparing the allergenicity of pollen fractions is emphasized.
D000485:Allergens; D005591:Chemical Fractionation; D006801:Humans; D007525:Isoelectric Focusing; D011058:Pollen; D011852:Radioallergosorbent Test; D012882:Skin Tests
D000485:Allergens