[Critical analysis of the radioimmunological methods of determining creatine kinase isoenzyme MB (CK-MB), myoglobin (MG) and of LDH (H4) in ischemic cardiopathy].
We have studied 135 subjects of whom 100 were normal individuals; 10 with diagnosis of acute myocardial infarction (AMI); 10 with angina pectoris; 10 undergoing cardiac catheterism; 5 who underwent open heart surgery. To verify the radioimmunoassay usefulness of CPK cardiac isoenzyme (CK-RIA), of lactate dehydrogenase [LDH (H4)], of myoglobin (MG) in the diagnosis of ischemic disease, we have determined for serum samples: LDH (H4) by radioimmunoassay and HBDH by biochemical assay; CK by biochemical assay; CK-MB by biochemical and radioimmunological assay; MG by radioimmunoassay. The results indicate MG as a sensitive marker for the diagnosis of AMI. In fact serial serum determinations in patients with AMI showed myoglobin levels in 60% of the cases within 1 h after the onset of pain. The CK-RIA is the most sensitive test to evaluate infarct size and LDH (H4) conditioned by the amount of intracellular lactate is an useful test to evaluate myocardial anoxia.
D000208:Acute Disease; D000787:Angina Pectoris / Q000175:diagnosis; D006328:Cardiac Catheterization; D006348:Cardiac Surgical Procedures; D003327:Coronary Disease / Q000175:diagnosis* / Q000201:enzymology; D003402:Creatine Kinase / Q000097:blood*; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D009203:Myocardial Infarction / Q000175:diagnosis; D009211:Myoglobin / Q000097:blood*; D011863:Radioimmunoassay
D007527:Isoenzymes; D009211:Myoglobin; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase
Analisi critica delle metodiche radioimmunologiche per la determinazione della CK-MB, della MG e della LDH (H4) nelle cardiopatie ischemiche.