title: Use of alpha cyclodextrin to eliminate interference by nonesterified fatty acids in calcium measurements.
abstract: This study shows that nonesterified fatty acids cause underestimation of serum calcium by some colorimetric methods. The underestimation of calcium occurs infrequently, but can be as large as 20-30 mg/l. Preincubation of the sample with alpha cyclodextrin will eliminate this interference. The procedure is simple, inexpensive, and applicable to most clinical laboratories.
mesh_terms: D001322:Autoanalysis; D002118:Calcium / Q000097:blood*; D055598:Chemical Phenomena; D002621:Chemistry; D003124:Colorimetry / Q000379:methods; D003505:Cyclodextrins; D003912:Dextrins; D005230:Fatty Acids, Nonesterified; D006801:Humans; D012016:Reference Values; D013213:Starch
chemical_list: D003505:Cyclodextrins; D003912:Dextrins; D005230:Fatty Acids, Nonesterified; D013213:Starch; D002118:Calcium