Structure and stability of complexes between poly-5-bromouridylic acid and 2,9-dimethyladenine or 2-methyladenosine.
The complexes formed between poly-5-bromouridylic acid (poly(BU)) and 2,9-dimethyladenine (m2m9A) or 2-methyladenosine (m2Ado) were investigated. The stoichiometry of the m2m9A-poly(BU) complex was found to be 1:1 as determined by equilibrium dialysis measurements. The CD spectra of the complexes showed the formation of helically ordered structure. This is also confirmed by the sharp melting profiles. A comparison of the CD spectra with that of poly(m2A) . poly(BU) complex revealed a close relationship of the helical structures for these three types of complexes. It was observed that the m2m9A was able to form a thermally more stable complex with the poly(BU) than the m2Ado did.
D000225:Adenine / Q000031:analogs & derivatives*; D000241:Adenosine / Q000031:analogs & derivatives*; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D004355:Drug Stability; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D011072:Poly U / Q000031:analogs & derivatives*
C031088:2-methyladenosine; D011072:Poly U; C031089:poly-5-bromouridylic acid; C031087:2,9-dimethyladenine; D000225:Adenine; D000241:Adenosine