The protein sequenator: a new extraction principle and a metal reaction cup.
A simpler and more efficient extraction procedure is proposed for solvent extraction of the Edman degradation procedure in the protein sequenator. The method is less likely than the conventional one to cause wash-out of peptides or proteins from the reaction cup while the very small solvent volumes required substantially decrease the running costs of the instrument. It has been used successfully (98% repetitive yield) with a metal reaction cup in the sequenator.
D000595:Amino Acid Sequence; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D007202:Indicators and Reagents; D011506:Proteins; D012997:Solvents
D007202:Indicators and Reagents; D011506:Proteins; D012997:Solvents