[Sensitization of lymphocyte to tumor extract detected by macrophage migration inhibition test(MIT) in patients with cervical cancer (author's transl)].
In vitro cellular hypersensitivity to the tumor-associated antigens (TAA) in 30 patients with cervical cancer (12 cases with stage 1b and 18 cases with stage 11) was studied by the method of macrophage migration inhibition test (MIT). Tumor soluble extracts as antigen were obtained by sonication or 3M-KCL method. The results of MIF production was presented as a migration index (MI) by the following formula. (Formula: see text), The result of MI were as follows: 79.25 +/- 21.69 in patients with stage 1b, 79.50 +/- 14.65 with stage 11. A positive MI case (MIF positive) which was less than 75, was found in 8 cases (66.7%) with stage 1b and 5 cases (27.8%) with stage 11. Relationship between the result of MI and skin reactions were as follows: 75.83 +/- 17.30 in PPD positive (18 cases), 84.75 +/- 16.96 in PPD negative (12 cases); 78.70 +/- 18.24 in DNCB positive (20 cases), 84.75 +/- 16.96 in DNCB negative (10 cases); 77.93 +/- 15.79 in PHA positive (27 cases) and 97.33 +/- 24.79 in PHA negative (3 cases). The serial assay of MI were performed in 10 cases and MIF production disappeared within 6 weeks after curative operation in all cases. From these findings, the cellular immune response to autologous tumor extracts was found in this assay system.
D000951:Antigens, Neoplasm / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D002464:Cell Migration Inhibition; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D009361:Neoplasm Invasiveness; D012882:Skin Tests; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
D000951:Antigens, Neoplasm