Effect of bovine growth hormone and a partially pure preparation of somatomedin on various growth parameters in hypopituitary dwarf mice.
The growth-promoting effects of a partially purified preparation of somatomedin (12.7 units/mg) were compared with those of various doses of bovine GH (5, 20 and 80 micrograms/day) when injected into hypopituitary dwarf mice. Growth parameters studied were body-weight and tail-length velocities (calculated as the slope of a regression line fitted to daily measurements against time), uptake of 35SO4(2-) into costal cartilage in vivo and organ weights (heart, liver and kidney). In the first experiment somatomedin (6.4 units/day), bovine GH and 0.9% NaCl were injected once daily in a volume of 0.1 ml for 10 days. Treatment with bovine GH promoted a significant dose-dependent increase in body-weight and tail-length velocities and 35SO4(2-) uptake into costal cartilage in vivo. Somatomedin also promoted a significant increase in body-weight velocity and 35SO4(2-) uptake, both responses were between that observed with the lowest dose of bovine GH and control values. Somatomedin did not promote increase in tail-length velocity. Organ weights did not differ significantly between any of the treatment groups when expressed as mg/g body weight. In the second experiment somatomedin (a daily total of 21.6 units/day) and 0.9% NaCl were injected three times per day in a volume of 0.033 ml, bovine GH was again injected once daily in a volume of 0.1 ml, and the treatment period was 12 days. As in the first experiment all doses of bovine GH and somatomedin promoted a significant increase in body-weight velocity. These results are consistent with the somatomedin hypothesis.
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002356:Cartilage / Q000378:metabolism; D002417:Cattle; D004393:Dwarfism, Pituitary / Q000378:metabolism / Q000503:physiopathology*; D005260:Female; D006128:Growth / Q000187:drug effects*; D013006:Growth Hormone / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D013002:Somatomedins / Q000494:pharmacology*; D013431:Sulfates / Q000378:metabolism
D013002:Somatomedins; D013431:Sulfates; D013006:Growth Hormone