title: The analysis of arildone in plasma, urine and feces by gas--liquid chromatography with electron-capture detection.
abstract: The analysis of arildone in plasma, urine and feces by gas--liquid chromatography with electron-capture detection is described. O-(2,3,4,5,6-Pentafluorobenzyl)hydroxylamine is the derivatizing agent for the plasma and urine analysis; 3-nitrophenylhydrazine is utilized for fecal analysis. The mean (+/- S.E.) minimum quantifiable level of arildone was 1.4 (+/- .02) ng/ml in urine, 6.4 (+/- 0.1) ng/ml in plasma, and 12.6 (+/- 1.0) ng/g in feces. The chromatographic response was linear in the range of 0 and 10--120 ng/ml for plasma, 0 and 2.5--20 ng/ml for urine and 0 and 25-250 ng/g for feces. The estimated overall precision of the assay was 5.5%, 64% and 8.9% in urine, plasma and feces, respectively.
mesh_terms: D000998:Antiviral Agents / Q000097:blood* / Q000652:urine; D005243:Feces / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007659:Ketones / Q000097:blood* / Q000652:urine; D012016:Reference Values
chemical_list: D000998:Antiviral Agents; D007659:Ketones; C020706:arildone