[Preparation of tritium-labelled carminomycin and rubomycin by the isotope-exchange method with tritiated water and the determination of the label stability in aqueous solutions].
A simple procedure for preparation of tritiated anthracycline antibiotics, such as rubomycin and carminomycin is described. The method is based on isotope exchange with tritiated water at 100 degrees C. The specific activity of the preparations is several hundreds decays a minute per 1 microgram. The stability of the antibiotic label in aqueous solutions at various pH values was determined. At pH 7.2 and a temperature of 37 degrees C the loss of the tritium by the end of the 1st, 2nd, 3rd, 4th and 7th day amounted to 7.1, 11, 14, 19, and 24 per cent, respectively. It is concluded tht tritiated antibiotics prepared with the method of tritiated water exchange may be used in biomedical investigations.
D002360:Carubicin / Q000494:pharmacology*; D003630:Daunorubicin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004355:Drug Stability; D007553:Isotope Labeling / Q000379:methods*; D012996:Solutions; D014316:Tritium
D012996:Solutions; D014316:Tritium; D002360:Carubicin; D003630:Daunorubicin
Poluchenie mechennogo tritiem karminomitsina i rubomitsina metodom izotopnogo obmena s tritievoĭ vodoĭ i opredelenie stabil'nosti metki v vodnykh rastvorakh.