title: Characterization of two gangliosides of the paragloboside series from chicken skeletal muscle.
abstract: Two glucosamine-containing gangliosides, a ceramide pentasaccharide and ceramide heptasaccharide, have been purified from the gangliosides of chicken skeletal muscle. The lipids were extracted with a mixture of tetrahydrofuran and aqueous KCl and purified by anion-exchange and silicic acid column chromatography. Their structures were determined by specific enzymatic hydrolysis and methylation analysis. The proposed structures are sialosyl derivates of: (a) paragloboside, and (b) paragloboside with an additional lactosamine disaccharide: (a) NeuAc alpha 2 leads to 3Gal beta 1 leads to 4GlcNAc beta 1 leads to 3Gal beta 1 leads to 4GlcCer, (b) NeuAc alpha 2 leads to 3Gal beta 1 leads to 4GlcNAc beta 1 leads to 3Gal beta 1 leads to 4GlcNAc beta 1 leads to 3Gal beta 1 leads to 4GlcCer. The long base was predominantly sphingosine and the acyl groups were mainly palmitate, stearate and oleate.
mesh_terms: D000818:Animals; D002518:Ceramides / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D005677:G(M1) Ganglioside / Q000032:analysis; D005678:G(M2) Ganglioside / Q000032:analysis; D005679:G(M3) Ganglioside / Q000032:analysis; D005732:Gangliosides / Q000302:isolation & purification*; D009132:Muscles / Q000378:metabolism*; D009844:Oligosaccharides / Q000032:analysis; D010369:Pectoralis Muscles / Q000378:metabolism
chemical_list: D002518:Ceramides; D005679:G(M3) Ganglioside; D005732:Gangliosides; D009844:Oligosaccharides; D005678:G(M2) Ganglioside; D005677:G(M1) Ganglioside