title: High resolution gas chromatography mass spectrometry of the methyl esters of organic acids from uremic hemofiltrates.
abstract: The organic acid fraction of hemofiltrates was investigated in the form of methylates by glass capillary gas chromatography-mass spectrometry. The pattern obtained is similar to that of urinary organic acid methylates from healthy individuals. A marked difference was noted for N-phenylacetyl-alpha-aminoglutarimide, present in hemofiltrate at levels 50-100 times higher than those in urine. Analysis of hemofiltrate samples taken at different times during a hemofiltration with post-dilution technique revealed that the hemofiltrate concentration of most compounds was drastically reduced the course of the hemofiltration treatment. Compared to the other compounds, the reduction in hemofiltrate concentration of N-phenylacetyl-alpha-aminoglutarimide was extremely rapid.
mesh_terms: D000328:Adult; D001769:Blood; D002264:Carboxylic Acids / Q000097:blood*; D004952:Esters / Q000097:blood* / Q000652:urine; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D014462:Ultrafiltration; D014511:Uremia / Q000097:blood* / Q000652:urine
chemical_list: D002264:Carboxylic Acids; D004952:Esters