Protective effect of methionine against vinyl chloride-mediated depression of non-protein sulphydryls and cytochrome p-450.
Potentiality of a protective effect of methionine against hepatotoxic action of vinyl chloride (VC) was assessed on rats, induced with phenobarbital and thereafter exposed to 50 000 ppm VC for 5 h. This extremely high dosage of VC warranted the appearance of drastic symptoms of liver injury in the course of 20 h postexposure. DL-Methionine in a dose of 1 g/kg given by gavage prior to the VC-exposure counteracted both the BC-provoked depletion of non-protein sulphydryls and destruction of cytochrome P-450 in the liver. In the methionine-pretreated rats the phenobarbital-induced synthesis of microsomal proteins in the livers was maintained during the post-exposure period, contrary to the non-pretreated animals. It may be justified to apply methionine as a prophylactic agent in cases of occupational exposure to VC.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008715:Methionine / Q000494:pharmacology*; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D014752:Vinyl Chloride / Q000009:adverse effects*; D014753:Vinyl Compounds / Q000009:adverse effects*
D013438:Sulfhydryl Compounds; D014753:Vinyl Compounds; D003577:Cytochrome P-450 Enzyme System; D008715:Methionine; D014752:Vinyl Chloride; D010634:Phenobarbital