Evaluation of a high-performance liquid chromatography method for isolation and quantitation of cholesterol and cholesteryl esters.
A rapid method for the separation and quantitation of unesterified cholesterol and cholesteryl esters by high-performance liquid chromatography is described. All of the cholesteryl esters typically present in plasma were resolved on Zorbax ODS reversed-phase columns using a linear gradient of water (3%-0%) in acetonitrile-tetrahydrofuran 65:35 (v/v) as eluting solvent. Results obtained by this method show good agreement with chemical and gas-liquid chromatographic methods for quantitation of cholesterol and determination of cholesteryl ester percentage compositions. High-performance liquid chromatography provides the only available method for the separation and quantitation of individual cholesteryl esters from lipid extracts of biological samples.
D000818:Animals; D002522:Chlorocebus aethiops; D002784:Cholesterol / Q000302:isolation & purification*; D002788:Cholesterol Esters / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008297:Male
D002788:Cholesterol Esters; D002784:Cholesterol