Some improvements in the synthesis of DNA of biological interest.
In a brief summary the improvements introduced during synthesis of a minigene coding for the peptide hormone angiotensin II are outlined. Furthermore some recent improvements are reviewed: comprehensive study of N-protecting groups for the heterocyclic bases, effective 3'-O-phosphorylation using phosphomonochloridates and molecular sieves, a quick and mild detritylation procedure without any depurination using ZnBr2, a convenient tritylation method using molecular sieves, introduction of a sugar spray reagent for a simpler interpretation of tlc, an improved two-step-one-flask procedure for the synthesis of fully protected triester intermediates, an introduction of new phosphate protecting groups removable by beta-elimination in homogeneous phase, synthesis of the internucleotidic phosphotriester linkage using phosphomonoazolide derivatives of deoxynucleosides, versatile methods for anchoring nucleosides to a polymer support and a computerized nucleic acid synthesizer.
D000804:Angiotensin II / Q000235:genetics; D001483:Base Sequence; D003090:Coliphages / Q000235:genetics; D004247:DNA / Q000138:chemical synthesis*; D005796:Genes; D005815:Genetic Code; D006801:Humans; D007202:Indicators and Reagents; D008722:Methods; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D009876:Operon
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D000804:Angiotensin II; D004247:DNA