POlygalacturonic acid transeliminase production by Azospirillum species.
Polygalacturonic acid transeliminase (PATE) was produced by all of six Azospirillum strains studied. Characteristics were similar to those of PATE from other bacteria: activity was maximal at pH 8.0 and was stimulated by CaCl2. Polygalacturonic acid was used more readily than pectin as a substrate. Polygalacturonic acid in the medium stimulated PATE production by several but not all strains. In all cases some of the PATE produced in cultures remained bound to cell walls. In one strain, most remained cell wall bound. When nitrogen was supplied as amino acids rather than ammonium salts, the ratio of free to bound enzyme was increased. The strains studied varied considerably to response to nutrient amendments and in maximum PATE activity.
D001419:Bacteria / Q000201:enzymology*; D002118:Calcium / Q000494:pharmacology; D002473:Cell Wall / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D010368:Pectins; D011133:Polysaccharide-Lyases / Q000096:biosynthesis*; D013045:Species Specificity; D013379:Substrate Specificity
D010368:Pectins; D011133:Polysaccharide-Lyases; C023911:pectate lyase; D002118:Calcium; C003181:polygalacturonic acid