Plasma glucose concentrations of whole blood, as determined with a multilayer-film analytical element.
A new type of multilayer film analytical element--consisting of a spreading layer, a blocking layer, an enzymic reagent layer, and a transparent layer--has been developed for blood glucose determination. After a spot of whole blood is dropped on the film, the plasma glucose concentration is determined in 6 min without further manipulations. Precise measurement of sample volume spotted on the film is not necessary for good results, nor is knowledge of the hematocrit in the range 10-46%. The minimum concentration of glucose detectable by this method is 100 mg/L. The coefficients of variation, within-run and between-run, were all less than 2.5% for 800-4000 mg/L glucose concentrations. Macromolecules and hydrophobic substances in blood did not interfere. This method seems suitable for emergency use, especially.
D001786:Blood Glucose / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D005069:Evaluation Studies as Topic; D005949:Glucose Oxidase; D006593:Hexokinase; D006801:Humans; D008832:Microchemistry; D004364:Pharmaceutical Preparations; D011933:Reagent Kits, Diagnostic
D001786:Blood Glucose; D004364:Pharmaceutical Preparations; D011933:Reagent Kits, Diagnostic; D005949:Glucose Oxidase; D006593:Hexokinase