The determination of N1-acetylspermine in mouse liver.
N1-Acetylspermine has been postulated to be an intermediate in the conversion of spermine to spermidine. This compound, together with N1-acetylspermidine has now been detected in the liver of mice which were pretreated with tetrachloromethane. The following methods were used for the identification of N1-acetylspermine: (a) High-pressure liquid-chromatography of the non-derivatized amines on a reversed-phase column, using octane sulfonate for ion-pairing. (b) Thin-layer chromatography of the dansyl derivatives. (c) Mass spectrometry of the dansyl derivatives. Both chromatographic methods allowed the quantitative estimation of N1-acetylspermine and N1-acetylspermidine in the liver of tetrachloromethane-treated animals.
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D008099:Liver / Q000032:analysis*; D008297:Male; D013058:Mass Spectrometry; D051379:Mice / Q000378:metabolism*; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000032:analysis; D011073:Polyamines / Q000378:metabolism; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D000097667:Polyamine Oxidase
D000587:Oxidoreductases Acting on CH-NH Group Donors; D011073:Polyamines; D013095:Spermidine; D013096:Spermine; D000097667:Polyamine Oxidase; C029452:N'-acetylspermine