[Dynamics of the mitotic cycle and mitosis anomalies in 2-mercaptoethanol action on pig embryo kidney tissue culture cells].
The effect of 2-mercaptoethanol on the dynamics of mitotic cycle was examined in cells of a porcine embryo kidney culture. 2-mercaptoethanol is shown to block cell division in C-metaphase-like figures. After the agent removal the block was stopped, and cells proceeded to a normal metaphase again. The presence of 2-mercaptoethanol does not prevent the S/G2 transition, the entry of cells to the prophase and then to the normal metaphase. At the same time, the length of G2-period of interphase appears to remain normal. In a porcine embryo kidney culture 2-mercaptoethanol induced multipolar mitoses and mitoses without cytokinesis. The number of multipolar mitoses increased with the increase in the time of incubation.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000187:drug effects*; D008623:Mercaptoethanol / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects*; D013552:Swine; D013997:Time Factors
D008623:Mercaptoethanol
Dinamika mitoticheskogo tsikla i anomalii mitoza pri deĭstvii 2-merkaptoétanola na Kletki kul'tury tkani SPEV.