Fluorescence emission by wild-type- and mutant-strains of Rhodopseudomonas capsulata.
Absorption and fluorescence emission spectra of Rhodopseudomonas capsulata, strains 37b4 (wild type), A1a+ (blue-green mutant strain), Y5 (phototroph negative, having only B-800--850 bacteriochlorophyll-carotenoid-protein complex) at 4 K, 77 K and 300 K were measured. The fluorescence emission at 890 nm of the B-870 bacteriochlorophyll band dominates the emission of other spectral forms of the strains 37b4 and A1a+, while in strain Y5 a fluorescence emission band at 865 nm of the B-850 bacteriochlorophyll dominates. Very little fluorescence was observed at 805 nm. A linear relation between relative fluorescence intensity and the exciting light intensity was observed. The integrated fluorescence yield increased as the temperature was lowered from 300 K to 4 K. The results are discussed in the light of the arrangement of pigment molecules in the membrane and the process of energy migration within the photosynthetic apparatus.
D001429:Bacteriochlorophylls / Q000378:metabolism; D004735:Energy Transfer; D005453:Fluorescence; D009154:Mutation; D010788:Photosynthesis; D010860:Pigments, Biological / Q000378:metabolism; D012241:Rhodopseudomonas / Q000235:genetics / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis; D013696:Temperature
D001429:Bacteriochlorophylls; D010860:Pigments, Biological