[Fibrinolytic activity and effect of anti-fibrinolytic agents on the growth of cloned cell lines in vitro (author's transl)].
Fibrinolytic activity and effect of anti-fibrinolytic agents on the growth of cloned cell lines derived from experimental uterine and ovarian adenocarcinomas in rat were studied in tissue culture. 1) All cloned cell lines derived from uterine (HTP/Cl and LTP/C3) and ovarian (ROT58/C3) adenocarcinomas produced plasminogen activator in vitro. 2) The plasminogen activator secreted by these cloned cell lines was immunologically distinct from human urokinase. 3) Colony forming rate of ROT58/C3 cells was inhibited by anti-fibrinolytic agents in dose-dependent manner. However, t-AMCHA at low concentrations of 10 to 100 microgram/ml promoted the colony forming rate. 4) Human urokinase at concentration of 12 IU/ml enhanced the colony forming rate of ROT58/C3 cells, whereas 120 IU/ml suppressed it. 5) Culture medium with plasminogen-deprived fetal bovine serum suppressed the colony forming rate of ROT58/C3 cells. These results indicate that the growth of malignant tumor cells is influenced by coagulative and fibrinolytic system and anti-fibrinolytic agents are effective to the treatment of malignant tumors.
D000230:Adenocarcinoma / Q000097:blood* / Q000473:pathology; D000818:Animals; D000933:Antifibrinolytic Agents / Q000494:pharmacology*; D002460:Cell Line; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D005260:Female; D005342:Fibrinolysis; D009374:Neoplasms, Experimental / Q000097:blood / Q000473:pathology; D010051:Ovarian Neoplasms / Q000097:blood* / Q000473:pathology; D051381:Rats; D014594:Uterine Neoplasms / Q000097:blood* / Q000473:pathology
D000933:Antifibrinolytic Agents