Mouse spermatogonia exposed to a high, multiply fractionated dose of a cancer chemotherapeutic drug: mutation analysis by electrophoresis.
Male mice of the DBA/2J strain were injected with procarbazine at a dose of 200 mg/kg body weight twice weekly until an accumulated dose of 2400 mg/kg was reached. A concurrent control group, injected only with the vehicle (saline) was also established. Most of the treated animals died as a result of exposure and all survivors became temporarily sterile. After regaining fertility the few survivors were repeatedly mated with C57BL/6J females over several weeks time to generate a population of F1 animals. The parental animals and the F1 were subsequently analyzed by electrophoresis for the occurrence of newly arisen mutations of spermatogonial origin. A mutation in the gene Pep-3 was found.
D000818:Animals; D003433:Crosses, Genetic; D004305:Dose-Response Relationship, Drug; D004586:Electrophoresis / Q000379:methods*; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D008811:Mice, Inbred DBA / Q000235:genetics; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens; D011344:Procarbazine / Q000494:pharmacology*; D013093:Spermatogonia / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*
D009153:Mutagens; D011344:Procarbazine