DNA/anti-DNA complexes: correlation of size and complement fixation.
The hydrodynamic sizes of antibody/3H-dsDNA immune complexes are correlated with their ability to fix complement in a red blood cell-complement linked binding assay. The results with PM2 dsDNA and a sonicated preparation (with a molecular weight of approximately 5.10(5) suggest that packing considerations are also important in the complement binding reaction. The relevance of these findings with respect to the role of antibody/dsDNA immune complexes in the pathogenesis of glomerulonephritis in systemic lupus erythematosus is discussed.
D000936:Antigen-Antibody Complex / Q000096:biosynthesis / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D055598:Chemical Phenomena; D002621:Chemistry; D003168:Complement Fixation Tests; D004247:DNA / Q000276:immunology*; D005921:Glomerulonephritis / Q000150:complications / Q000276:immunology; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000150:complications / Q000276:immunology*; D014461:Ultracentrifugation
D000936:Antigen-Antibody Complex; D000941:Antigens; D004247:DNA