Automated discrete kinetic method for erythrocyte acetylcholinesterase and plasma cholinesterase.
We describe an automated kinetic method for erythrocyte acetylcholinesterase (EC 3.1.1.7) and plasma cholinesterase (EC 3.1.1.8) based on Ellman's colorimetric method. Quinidine sulfate is used as an inhibitor of plasma cholinesterase during the measurement of erythrocyte acetylcholinesterase activity, obviating the need for washing the erythrocytes before lysis. Results by this method are compared with those obtained by the electrometric delta pH method of Michel. To emphasize the need for measuring both erythrocyte acetylcholinesterase and plasma cholinesterase activity in workers exposed to organophosphate pesticides, we present a study of serial activities of both enzymes in a person accidentally exposed to demeton-S-methyl.
D000110:Acetylcholinesterase / Q000097:blood*; D001322:Autoanalysis; D002802:Cholinesterases / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007700:Kinetics; D008722:Methods; D062025:Organophosphate Poisoning; D063086:Organothiophosphates; D011802:Quinidine / Q000494:pharmacology
D007202:Indicators and Reagents; D063086:Organothiophosphates; D000110:Acetylcholinesterase; D002802:Cholinesterases; C030064:demeton-S-methyl; D011802:Quinidine