Radioimmunoassay of cyclazocine and stereospecificity of antibody.
A new radioimmunoassay, using 3H-cyclazocine, rabbit antiserum and charcoal-dextran separation of bound and free cyclazocine, for the direct analysis of serum cyclazocine is described. This method, which is specific for cyclazocine and has a detection limit of ca. 25 pd/assay tube, was successful in determining the cyclazocine level in the sera of beagles intramuscularly injected with 3 or 10 microgram/kg cyclazocine. The drug half-life was 90 min; the apparent distribution volumes were 4.0 and 5.26 liter/kg, respectively. One of the antisera raised in rabbits immunized with dl-cyclazocine derivative-BSA conjugates was highly specific for l-cyclazocine.
D000818:Animals; D000918:Antibody Specificity; D003496:Cyclazocine / Q000097:blood* / Q000276:immunology; D004285:Dogs; D005260:Female; D006168:Guinea Pigs; D011863:Radioimmunoassay / Q000379:methods*; D013237:Stereoisomerism
D003496:Cyclazocine