Effect of amniotic fluid and fetal bovine serum on the morphogenesis of mouse duodenal villi in organ culture.
When duodenal explants of 15-day mouse foetuses are cultured in Trowell T8 medium during 72 h, villi do not develop. If mouse amniotic fluid (25%) is added to the same medium, short villi appear after 12 h of culture and medium size villi are seen at 48 h. Bovine amniotic fluid and fetal bovine serum promote cell growth but morphogenesis of intestinal villi is far less stimulated.
D000653:Amniotic Fluid / Q000502:physiology*; D000818:Animals; D003470:Culture Media; D004386:Duodenum / Q000166:cytology / Q000196:embryology*; D006133:Growth Substances / Q000097:blood*; D007413:Intestinal Mucosa / Q000166:cytology; D051379:Mice; D009024:Morphogenesis / Q000187:drug effects; D009924:Organ Culture Techniques
D003470:Culture Media; D006133:Growth Substances