Human immunoglobulin M purification by affinity chromatography on protein A--Sepharose.
A method is described for preparing IgM from normal serum in two chromatographic steps. A gel filtration column gives a macroglobulin peak containing the IgM, purified by affinity chromatography on protein A--Sepharose. Immunoglobulin recovery is around 50%.
D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007075:Immunoglobulin M / Q000302:isolation & purification*; D011863:Radioimmunoassay; D012685:Sepharose; D013205:Staphylococcal Protein A
D007075:Immunoglobulin M; D013205:Staphylococcal Protein A; D012685:Sepharose