Measurement of plasma progesterone and its O-methyloxime by gas chromatography-nitrogen detection.
A routine method for the quantitation of plasma progesterone, using only 1 ml of sample, has been developed based on gas chromatography-nitrogen detection of the O-methyloxime derivative. Simple solvent extraction of progesterone from plasma using cyclohexane as solvent and medrogestone as internal standard allows measurement at the 3 nmol/l level, while the coefficient of variation at 16, 64, and 127 nmol/l progesterone is 14, 7.5, and 6.5% respectively. Comparison with a radioimmunoassay gave a linear regression equation of Y = 0.84x + 1.4, Syx = 7.8, r = 0.9255 (n = 55).
D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D006898:Hydroxylamines; D008524:Medrogestone; D011374:Progesterone / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood*; D011863:Radioimmunoassay
D006898:Hydroxylamines; D008524:Medrogestone; C030842:progesterone-20-(O-methyloxime); D011374:Progesterone; C005214:methoxyamine