Deoxyribonucleic acid (DNA) content of morphologically different sperm types from normal and subfertile human males.
20 male patients (G2), affected by idiopathic oligozoospermia, and 10 normal fertile males (G1) were investigated. The DNA content of 50 Feulgen stained sperm heads per subject was determined, using single cell photometry. In a second step area, circumference, length, and width of the same heads were measured, using a semi-automatic image analysis system. Considering only morphologically normal heads, the mean DNA content is only slightly smaller in G2 than in G1. On the contrary, the DNA variation is strongly higher in G2 than in G1. Unfortunately, morphologically defect heads show an increased DNA variation, too. Thus, determining the DNA variation of a semen, containing many morphologically defect heads, a combined determination of the DNA content and head morphology is necessary. This can only be achieved by single cell cytophotometry, which is therefore superior to flow cytophotometry as regards this problem. Many heads with a strongly abnormal DNA content do not show any morphological abnormalities. These subcellular changes can only be detected by the determination of the DNA content.
D004247:DNA / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000378:metabolism*; D013077:Sperm Head / Q000032:analysis*; D013094:Spermatozoa / Q000032:analysis*
D004247:DNA