C6--C10-dicarboxylic aciduria in starved, fat-fed and diabetic rats receiving decanoic acid or medium-chain triacylglycerol. An in vivo measure of the rate of beta-oxidation of fatty acids.
Administration of decanoic acid to rats resulted not only in elevated urinary excretions of the C10-dicarboxylic acid (sebacic acid), but also in highly elevated excretions of the beta-oxidation products C8- and C6-dicarboxylic acids (suberic and adipic acids). Activation of the lipid metabolism by starvation, fat-feeding and experimental diabetes increased the excretions of adipic acid and decreased the excretions of sebacic acid, i.e. the rate of oxidation of fatty acids was correlated to the adipic : sebacic acid ratio in urine. Compared with nondiabetic unstarved rats the adipic : sebacic acid ratio was elevated 2--3-, 8--16-, 5--19-, and 22--88-times in rats which were, respectively, starved for 2 days, 4 days, on a fat-diet for 4 days, and ketotic due to streptozotocin-induced diabetes. All rats with ratios above 10 were ketotic (urinary excretions of 3-hydroxybutyric acid over 500 microgram/mg creatinine) and all rats with ratios below 4 were nonketotic, while ketosis was a variable finding in rats with intermediary ratios. Similar changes in the ratio of excreted dicarboxylic acids were found when medium-chain triacylglycerols were fed instead of decanoic acid.
D000138:Acidosis / Q000652:urine*; D000272:Adipates / Q000652:urine; D000818:Animals; D002210:Caprylates; D003652:Decanoic Acids / Q000652:urine; D003921:Diabetes Mellitus, Experimental / Q000652:urine*; D003998:Dicarboxylic Acids / Q000652:urine*; D004041:Dietary Fats; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D007662:Ketosis / Q000139:chemically induced / Q000652:urine*; D010084:Oxidation-Reduction; D051381:Rats; D013217:Starvation; D014280:Triglycerides
D000272:Adipates; D002210:Caprylates; D003652:Decanoic Acids; D003998:Dicarboxylic Acids; D004041:Dietary Fats; D005227:Fatty Acids; D014280:Triglycerides; C031071:decanoic acid; C005738:suberic acid; C029900:adipic acid; C011107:sebacic acid