Lactate dehydrogenase isoenzyme 1 with the centrifugal analyzer after immunochemical removal of other isoenzymes.
We describe a centrifugal analyzer method for measuring the LD-1 isoenzyme of lactate dehydrogenase (EC 1.1.1.27) in serum, after immunochemical precipitation of the other four isoenzymes. Enzymic activity was measured kinetically at 30 degrees C with the pyruvate-to-lactate assay. The method for LD-1 was linear to 1000 U/L. The precision (CV) of the assay was 1.0--2.2% within-run and 3.1--4.5% day-to-day. The reference interval was 26--73 U/L (n = 51), corresponding to 21--35% of total LD activity.
D000936:Antigen-Antibody Complex; D003720:Densitometry / Q000379:methods; D006801:Humans; D007106:Immune Sera; D007527:Isoenzymes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000097:blood* / Q000302:isolation & purification; D011933:Reagent Kits, Diagnostic
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007527:Isoenzymes; D011933:Reagent Kits, Diagnostic; D007770:L-Lactate Dehydrogenase