Comparison between the specificity of primary and secondary killer cells against alloantigens and hapten-modified syngeneic lymphoid cells.
Cytotoxic responses of lymphoid cells from different mouse strains against syngeneic cells modified with the haptens fluorescein isothiocyanate and trinitrophenyl were investigated. Mic of the H-2k strain demonstrated strong primary in vitro hapten-specific cytotoxicity reactions, which were H-2 restricted and involved the Kk specificity. However, cells from H-2d and H-2b mice developed hapten-specific cytotoxic reactions that showed H-2 cross-reactivity. This cross-reactivity, with regard to the restriction element, was particularly evident with cells from mice that had been immunized in vivo. No cross-reaction was observed between the two haptens, and Dd target cell antigens. Similar cross-reactions were demonstrated in vitro experiments in which secondary in vitro responses were induced by stimulation with cross-reacting H-2 antigens. This finding was also investigated in allogeneic cytotoxicity. In vitro induced responses resulting in relatively weak specific cell-mediated lympholysis reactions were H-2 specific, whereas secondary in vitro responses demonstrated cross-reactivity between Dd and Db antigens. In these test systems, cross-stimulation was also demonstrated in secondary in vitro responses. These results are discussed in terms of similarities of T cell recognition of hapten-modified self antigens and of alloantigens.
D000818:Animals; D000918:Antibody Specificity; D002478:Cells, Cultured; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000276:immunology; D006241:Haptens / Q000276:immunology*; D007519:Isoantigens / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D013045:Species Specificity
D006183:H-2 Antigens; D006241:Haptens; D007519:Isoantigens