Proliferation and synapse formation of neuroblastoma glioma hybrid cells: effects of glia maturation factor.
Glia maturation factor (GMF), extracted from bovine brain, stimulated DNA synthesis and proliferation of glioma cells and hybrid cells derived from glioma and neuroblastoma cells (NG108-15), but had no effect on neuroblastoma cells. The synapse formation of NG108-15 cells with rat striated myotubes was lower in the presence of GMF than the control and also lower after treatment with prostaglandin E1 (PGE1) plus theophylline, indicating that GMF did not induce functional differentiation of NG108-15 cells. The results show that expression of mitogenic action for GMF in the hybrid cells is a property derived from the glioma parent, and that NG108-15 is therefore an excellent model for studying glial-neuronal interactions.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D020904:Glia Maturation Factor; D005910:Glioma / Q000378:metabolism*; D006822:Hybrid Cells / Q000187:drug effects; D051379:Mice; D008938:Mitosis / Q000187:drug effects*; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009447:Neuroblastoma / Q000378:metabolism*; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology; D051381:Rats; D013569:Synapses / Q000187:drug effects*; D013806:Theophylline / Q000494:pharmacology
D004273:DNA, Neoplasm; D020904:Glia Maturation Factor; D009419:Nerve Tissue Proteins; D011459:Prostaglandins E, Synthetic; D013806:Theophylline