[3H]Spiperone binding sites in brain: autoradiographic localization of multiple receptors.
[3H]Spiperone ([3H]SP) binding sites were localized by light microscopic autoradiography, after in vitro labeling. The kinetic and pharmacological characteristics of these binding sites were studied in slide-mounted sections of rat forebrain, and optimal labeling conditions were defined. Autoradiograms were obtained by apposing emulsion-coated coverslips to labeled sections. Differential drug sensitivity allowed the selective displacement of [3H]SP from dopamine receptors by ADTN, from serotonin receptors by cinanserin, from both by haloperidol and from unique spiperone sites by unlabeled spiperone. The various sites presented a differential anatomical localization. For example, only dopaminergic sites were found in the glomerular layer of the olfactory bulb; only serotonergic sites were found in lamina IV of the neocortex, and a high concentration of unique spiperone sites was found in parts of the hippocampus.
D000818:Animals; D001345:Autoradiography; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D002090:Butyrophenones / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D007700:Kinetics; D008297:Male; D051381:Rats; D011954:Receptors, Dopamine / Q000378:metabolism*; D011955:Receptors, Drug / Q000378:metabolism; D013134:Spiperone / Q000378:metabolism*; D014316:Tritium
D002090:Butyrophenones; D011954:Receptors, Dopamine; D011955:Receptors, Drug; D014316:Tritium; D013134:Spiperone