Comparative study of chromatographic and non-chromatographic procedures in the assay of serum 25-hydroxyvitamin D.
Chromatographic and non-chromatographic procedures in the assay of serum 25-hydroxyvitamin D are compared. Samples extracted with chloroform/methanol were analysed using a competitive protein binding assay, with or without prior chromatography. Non-chromatographic methods constantly gave higher values than chromatographic methods. It is concluded that chromatography is an essential step in the preparation of extracts for the assay of 25-hydroxyvitamin D.
D000818:Animals; D002725:Chloroform; D002845:Chromatography; D006887:Hydroxycholecalciferols / Q000097:blood*; D000432:Methanol; D008722:Methods; D051381:Rats; D014808:Vitamin D Deficiency / Q000097:blood
D006887:Hydroxycholecalciferols; D002725:Chloroform; D000432:Methanol