Specific estrogen receptor binding and biological effects of 16 alpha-iodoestradiol on human breast cancer cells.
16 alpha-Iodoestradiol was evaluated as an estrogen in MCF-7 and ZR-75-1 human breast cancer cells. 16 alpha-[125I]Iodoestradiol binds to equivalent numbers of specific estrogen receptors with a comparable binding affinity and similar sucrose density gradient behavior. 16 alpha-Iodoestradiol translocates receptor sites to the nucleus and induces a full range of estrogenic effects including thymidine incorporation, cell growth, and progesterone receptor. Because of its high specific activity, this compound may have unique applications on imaging of estrogen receptor-containing cells and specific receptor-mediated cytotoxicity.
D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects; D004958:Estradiol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D005260:Female; D006801:Humans; D007700:Kinetics; D011960:Receptors, Estrogen / Q000378:metabolism*
D011960:Receptors, Estrogen; D004958:Estradiol; C024871:16 alpha-iodoestradiol