[Phosphorus nuclear magnetic resonance studies of the energetic state and of the intracellular pH of the isolated rat heart in the course of ischemia (author's transl)].
Continuous measurements of high energy phosphate compounds and intracellular pH in perfused, beating rat hearts, were performed by 31P nuclear magnetic resonance (NMR). Hearts were placed in a 15 mm NMR tube and perfused at 28 degrees C by conventional methods with a phosphate-free solution. Phosphorus NMR spectra were recorded at 101,3 MHz in a Brucker WP 250 spectrometer. Global mild ischemia was achieved by reducing the coronary flow to 1/10 of its initial value. Changes in creatine phosphate (CP) and inorganic phosphate (Pi) levels and intracellular pH (pHi) were monitored in the course of a 50 min ischemia and during the post-ischemic phase. When the breakdown of CP was less than 30%, the decrease in pHi was about 0.1-0.2 pH unit; for a greater CP decrease, the fall in pHi was about 1 pH unit.
D000818:Animals; D003327:Coronary Disease / Q000378:metabolism*; D004734:Energy Metabolism; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009206:Myocardium / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D051381:Rats
D010758:Phosphorus
Etude par résonance magnétique nucléaire (RMN) du 31P, de l'état énergétique et des modifications du pH intracellulaire au cours de l'ischémie du coeur isolé de rat.