title: Increased haemopoietic cell survival in vitro induced by a human marrow fibroblast factor.
abstract: Human marrow fibroblasts were grown in vitro and examined for effects on human and mouse haemopoietic cells. When human marrow cells were incubated with fibroblasts or with fibroblast-conditioned medium for 1 week and then assayed for committed granulocyte/monocyte (CFU-c) and erythroid (BFU-e) progenitor cells, the numbers of CFU-c and BFU-e were considerably increased compared with controls. In contrast, human marrow-fibroblast-conditioned medium, when added directly to CFU-c or BFU-e assay cultures, had no effect on colony formation by these progenitor cells. As these results suggest that the fibroblast-derived factor may be acting on a relatively primitive progenitor cell, possibly a pluripotent haemopoietic stem cell, the effect of this factor on mouse pluirpotent haemopoietic stem cells (CFU-s) was examined. Human marrow-fibroblast-conditioned medium considerably enhanced CFU-s survival after a 24 h incubation and increased the proportion of CFU-s in cell-cycle. The increase in CFU-s survival depended on the concentration of the fibroblast-conditioned medium but not on the age of the fibroblast culture. The evidence suggests therefore that human marrow fibroblasts produce a factor that acts on a human myeloid progenitor cell more primitive than BFU-e and CFU-c, possibly the pluripotent haemopoietic stem cell.
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000032:analysis*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004912:Erythrocytes / Q000187:drug effects; D005347:Fibroblasts / Q000032:analysis; D006098:Granulocytes / Q000187:drug effects; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D008297:Male; D051379:Mice; D009000:Monocytes / Q000187:drug effects; D013997:Time Factors
chemical_list: D006918:Hydroxyurea