[The effects of fosfomycin on antigenicity (author's transl)].
The antigenicity of fosfomycin (FOM), a new antibiotic agent, was studied. The binding of 3H-FOM with human serum protein was measured by the short term dialysis method. The binding was very weak only by 2.16% and reversible. The rabbits were immunized subcutaneously by fosfomycin as sodium salt (FOM-Na) with FREUND'S complete adjuvant. Antigenicity of IgG was examined by means of agar gel precipitation, passive cutaneous anaphylaxis and passive hemagglutination. To study the antigenicity of IgE, the BALB/cA mice were immunized intraperitoneally by FOM-Na with aluminum hydroxide gel and, passive cutaneous IgG and IgE. Consequently, it can be concluded that FOM does not conjugate with human serum protein to form its hapten and has not antigenicity.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology*; D000917:Antibody Formation; D001798:Blood Proteins / Q000378:metabolism; D005578:Fosfomycin / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D051379:Mice; D011485:Protein Binding; D011817:Rabbits
D000900:Anti-Bacterial Agents; D001798:Blood Proteins; D007074:Immunoglobulin G; D005578:Fosfomycin; D007073:Immunoglobulin E