title: Conformational states of yeast tRNA Phe in the complex with cognate and non cognate synthetases.
abstract: The influence of phenylalanyl-tRNA synthetase and seryl-tRNA synthetase on the conformation and structural kinetics of yeast tRNA Phe was investigated. Ethidium substituted for dihydrouracil at position 16 or 17 was used as a structural probe, showing the existence of three conformational states in tRNA. The distribution of states (T1, T2, T3) is changed only by the cognate synthetase towards T3 which probably is related to the X-ray structure. The binding of phenylalanyl-tRNA synthetase leads to an about 10-fold increase in the fast transition T1 in equilibrium or formed from T2 which has been assigned to changes in the anticodon loop conformation and to a 2-3 fold increase in the slow transition which probably extends to other parts of the tRNA molecule. The observed rates for the transition T2 in equilibrium or formed from T3 are close to that observed for the transfer of the activated phenylalanine to tRNA Phe. This raises the possibility that the conformational transition in tRNA is the rate limiting step in the charging reaction.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D004996:Ethidium; D005453:Fluorescence; D005456:Fluorescent Dyes; D007700:Kinetics; D008433:Mathematics; D009690:Nucleic Acid Conformation; D010652:Phenylalanine-tRNA Ligase / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012714:Serine-tRNA Ligase / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D012714:Serine-tRNA Ligase; D010652:Phenylalanine-tRNA Ligase; D004996:Ethidium