Addition of serum to the medium used for preparation of cell suspensions as a possible source of artifacts in cell-mediated reactions studied by means of the popliteal lymph node test.
Conditions for preparations of cells used in immunogenetic studies may substantially affect their properties and the results obtained. It was found that short-term incubation or a mere preparation of parental spleen cells in a medium containing either xenogeneic serum or concanavalin A (Con A) increase their ability (after footpad injection) to induce enlargement of the popliteal lymph node in (B10.LP x A/Ph)F1 hybrid recipients. Under similar conditions, enlargement of PLN could be induced also by syngenetic F1 hybrid cells. The greater lymphadenomegaly following the injection of parental or syngeneic cells prepared in serum-containing medium may be explained by the host reaction against additional foreign antigens rather than increased GVH reactivity or autoimmunity.
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology*; D006087:Graft vs Host Reaction; D007717:Knee; D008198:Lymph Nodes / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D003470:Culture Media