[Changes in enzyme activities in early passages of cultured fibroblasts].
The specific activities and variability coefficients (Wn) of specific activities of seven enzymes (5 lines) of cultured embryonic human fibroblasts were studied. The experiments were carried out on the 2nd--6th passage (between 12th and 19th passages); in each passage simultaneously up to 3-10 vials collected on the 8th cultivation day were analyzed. According to the Wn value, the enzymes under study can be divided into two groups, i.e. those with a low (less than or equal to 8%) and with a high (25-37%) Wn value. The data obtained are correlated with the division of enzymes and corresponding structural genes into "constituitive" and "inducible" ones. The differences between the enzymes and various lines with respect to the Wn value were revealed. The theoretical and practical significance of the observed changes of the enzyme activities in the cultured cells are discussed.
D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000201:enzymology; D004798:Enzymes / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000201:enzymology*; D005796:Genes; D006801:Humans; D008297:Male; D011247:Pregnancy
D004798:Enzymes
Razlichiia v variatsiiakh aktivnosteĭ fermentov v rannikh passazhakh kul'tiviruemykh fibroblastov.