Dexamethasone effects on liver pyruvate kinase.
Dexamethasone in the medium perfusing isolated rabbit livers caused a fast-acting and reversible effect on liver pyruvate kinase. The effect was to lower the assayable V activity (units/g tissue) without changing the concentration (nmol/g enzyme protein). In effect, glucocorticoid lowered the specific activity (units/nmol of enzyme) by direct action on liver. The effect on liver pyruvate kinase is mediated by a relatively stable alteration; 30 min after perfusate (with steroid) was replaced by perfusate (without steroid), the effect remained strongly evident.
D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D011770:Pyruvate Kinase / Q000378:metabolism*; D011817:Rabbits
D003907:Dexamethasone; D011770:Pyruvate Kinase