[Kinetics of the NADH regenerating system using bacterial formate dehydrogenase].
The main kinetic regularities of the NADH regeneration system functioning was studied. A theoretical interpretation of the dependence of the stationary reaction rate in a two-enzyme system with a common cofactor on the enzyme, cofactor and substrate concentrations and the catalytic parameters of individual enzymatic processes was obtained. A mathematical analysis of the dependences of the stationary rate on the content of each enzyme in the system at different activity ratios of each enzyme and within a broad range of initial cofactor concentrations was carried out. The kinetics of regeneration of native NADH and the NADH immobilized on a water-soluble 4-vinylpyridine and acroleine copolymer in a model two-enzyme formate dehydrogenase--NADH dehydrogenase system were investigated.
D000421:Alcaligenes / Q000201:enzymology*; D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D005560:Formate Dehydrogenases / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D009243:NAD / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D010084:Oxidation-Reduction
D009243:NAD; D005560:Formate Dehydrogenases; D000445:Aldehyde Oxidoreductases; D009245:NADH Dehydrogenase
Kineticheskoe izuchenie sistemy regeneratsii NADH na osnove bakterial'noĭ formitadegidrogenazy.