Studies on steroids. CLXV. Determination of isomeric catechol estrogens in pregnancy urine by high performance liquid chromatography with electrochemical detection.
A method for the determination of 2- and 4- hydroxylated estrone and estradiol in pregnancy urine by high-performance liquid chromatography with electrochemical detection (HPLC-ECD) is described. The urine catechol estrogens were deconjugated, purified by adsorption on alumina, and subjected to HPLC-ECD. Two pairs of isomeric catechol estrogens were distinctly separated on a microBondapak c18 column with acetonitrile-0.5% ammonium dihydrogen phosphate (pH 3.0). The amounts of these four compounds were satisfactorily determined with a quantitation limit of 1 ng using 4-hydroxy-16 oxoestradiol 17 acetate as an internal standard. The validity of the present methods for the determination of urine catechol estrogens was verified by the recovery test.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004958:Estradiol / Q000652:urine*; D004970:Estrone / Q000031:analogs & derivatives*; D005260:Female; D006801:Humans; D006894:Hydroxyestrones / Q000652:urine*; D007536:Isomerism; D011247:Pregnancy; D012016:Reference Values
D006894:Hydroxyestrones; D004970:Estrone; C026418:4-hydroxyestrone; D004958:Estradiol; C024980:2-hydroxyestrone