Determination of the primary metabolite of central nervous system norepinephrine, 3-methoxy-4-hydroxy-phenethyleneglycol, in mouse brain and brain perfusate by high-performance liquid chromatography with electrochemical detection.
Assays are described for the determination of picomole levels of 3-methoxy-4-hydroxy-phenethyleneglycol (MHPG) in mouse brain and in the perfusate of an intact mouse brain. High-performance liquid chromatography with electrochemical detection yielded a MHPG detection limit of 0.37 pmol. This technique offers a sensitive and inexpensive alternative to gas chromatography with mass spectrometry.
D000818:Animals; D001923:Brain Chemistry; D002490:Central Nervous System / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D006018:Glycols / Q000032:analysis*; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000032:analysis*; D051379:Mice; D009638:Norepinephrine / Q000378:metabolism
D006018:Glycols; D008734:Methoxyhydroxyphenylglycol; D009638:Norepinephrine