Correlation of chromosomal aberrations in a myeloma cell line with tumorigenicity in nude mice.
A human myeloma cell line (L 363) exhibiting several markers of malignancy including a 14q + chromosome marker was not tumorigenic in nude mice. Long-term cultivation in vitro resulted in new subclones with slight, well-defined chromosomal differences to the original line. Certain subclones turned out to have a growth advantage in vitro and became tumorigenic after transplantation in nude mice. A strong correlation between changes in the chromosome I constellation resulting in the threefold dose of long arm-material and the increased proliferative potential in both experimental systems was found.
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D002901:Chromosomes, Human, 13-15; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D009101:Multiple Myeloma / Q000473:pathology*