title: [Role of sulfhydryl groups in the inactivation mechanism of bacterial formate dehydrogenase].
abstract: Inactivation of formate dehydrogenase (EC 1.2.1.2) from gramnegative methanol-utilizing bacteria was studied. It was shown that the thermal inactivation of the enzyme occurs at temperatures above 50 degrees; at temperatures below 40 degrees the inactivation is due to metal ion-catalyzed oxidation of its sulfhydryl groups. A possible general mechanism of the enzyme inactivation is proposed.
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors*; D001419:Bacteria / Q000201:enzymology*; D005560:Formate Dehydrogenases / Q000037:antagonists & inhibitors*; D007700:Kinetics; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000032:analysis; D013696:Temperature
chemical_list: D013438:Sulfhydryl Compounds; D005560:Formate Dehydrogenases; D000445:Aldehyde Oxidoreductases
vernacular_title: Izuchenie roli sul'figdril'nykh grupp v mekhanizmi inaktivatsii bakteril'noĭ formiatdegidrogenazy.