A rapid chemical means for removing labile glycohemoglobin.
We have devised a rapid and convenient method of eliminating the contribution of labile glycosylated hemoglobin in the hemoglobin A1 assay. The procedure requires a 30-min incubation of erythrocytes in 30 mM semicarbazide and 12 mM aniline (pH 5, 38 degrees C). This chemical means of eliminating the labile fraction is as effective as a 14-h incubation in isotonic saline as measured with high-pressure liquid chromatography or electrophoresis methods. The removal of the labile glycosylated hemoglobin fraction preserves the assay as a reliable index of chronic metabolic control.
D000814:Aniline Compounds; D002851:Chromatography, High Pressure Liquid; D004587:Electrophoresis, Agar Gel; D004912:Erythrocytes / Q000032:analysis*; D006027:Glycosides / Q000032:analysis*; D006441:Hemoglobin A / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D012663:Semicarbazides
D000814:Aniline Compounds; D006027:Glycosides; D012663:Semicarbazides; D006441:Hemoglobin A; C023650:aniline