title: Blood freezing to nearly absolute zero temperature: -272.29 degrees C.
abstract: Human erythrocytes were collected in ACD anticoagulant, glycerolized with an equal volume of 8.6 M glycerol, 8 percent glucose, 1 percent fructose, and 0.3 percent Na2 EDTA solution, sealed in glass ampules, and frozen to -272.29 degrees C (0.86 degrees K) in liquid helium under high vacuum. Biological assays performed after thawing and three-stage dialysis to remove the glycerol showed less than 1 percent hemolysis. Intracellular potassium, 2,3 DPG, and ATP were within the normal range.
mesh_terms: D001769:Blood; D019411:Clinical Laboratory Techniques / Q000379:methods*; D004912:Erythrocytes; D005615:Freezing; D006801:Humans; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*