[Cinematographic and morphometric analysis of in vitro behavior of blastocysts of the mouse].
The behaviour of the mouse blastocyst in vitro was studied by time-lapse cinematography and analysed by morphometry. Three types of behaviour were observed: discontinuous expansion interrupted by rapid contractions and followed or not by hatching; practically continuous expansion followed by hatching. This demonstrates that the pulsatile activity of the blastocyst is not a necessary condition of hatching. Analysis of the rate and delay of hatching in vitro as compared to the conditions in utero is in favour of the existence of a uterine factor insuring partial lysis of the zona pellucida and helping the mechanical action of the blastocyst. Hatching by herniation through a reduced opening of the zona was occasionally observed. The process of in vitro implantation could be followed by cinematography.
D000818:Animals; D001755:Blastocyst / Q000502:physiology* / Q000648:ultrastructure; D002933:Cineradiography; D005260:Female; D051379:Mice; D014327:Trophoblasts / Q000502:physiology
Analyse cinématographique et morphométrique du comportement in vitro du blastocyste de la souris.