[The LISS technique-a more sensitive and faster method for erythrocyte antibody detection (author's transl)].
A low ionic strength solution (LISS) medium was compared with a conventional albumin fortified isotonic saline medium for the indirect antiglobulin test. The tests were performed in parallel with both media. The antisera used for the studies were commercial test sera of the specificities anti D, C, c, E, e, K, Le, Fy, Jk, Lu and sera of patients containing antibodies of the specificities anti D, CD, C, c, E, e, K, Le, Fy, Jk. Further observations were undertaken on the reaction of commercial antisera with mono- and polyspecific Coombs sera using the LISS technique. The investigations show a decrease in the incubation time and a slight increase in sensitivity of the reaction. Due to these advantages the LISS technique appears to be suitable for routine clinical antibody detection and cross-match reactions, especially in urgent cases.
D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D001788:Blood Grouping and Crossmatching; D001803:Blood Transfusion; D003298:Coombs Test; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology
D000906:Antibodies; D007106:Immune Sera
Die LISS-Technik, eine sensiblere und schnellere Nachweismethode erythrozytärer Antikörper.