Simultaneous determination of trimethoprim, sulphamethoxazole and N4-acetylsulphamethoxazole in serum and urine by high-performance liquid chromatography.
The simultaneous determination of trimethoprim, sulphamethoxazole and N4-acetyl-sulphamethoxazole in serum and urine by high-performance liquid chromatography using sulphafurazole as internal standard is described. The separation was achieved on a reversed-phase column employing acetic acid-methanol as the mobile phase with spectrophotometric detection at 230 nm. Precise simultaneous quantitative analysis of the relative components has been achieved at levels of 0.1 microgram/ml for trimethoprim and 1.0 microgram/ml for both sulphamethoxazole and its N4-acetyl metabolite using 1 ml of serum of urine.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D012016:Reference Values; D013420:Sulfamethoxazole / Q000031:analogs & derivatives* / Q000097:blood* / Q000652:urine; D014295:Trimethoprim / Q000097:blood* / Q000652:urine
C021282:N(4)-acetylsulfamethoxazole; D014295:Trimethoprim; D013420:Sulfamethoxazole