title: [Proliferation and DNA synthesis in a CHO-K1 cell culture with a varying serum concentration in the medium].
abstract: The possibility of using CHO-K1 cells for investigation of mechanisms involved in stimulation of cell proliferation by serum has been studied. The decrease of serum content in the cultural medium down to 0.5% causes the arrest of cell growth and the lowering of DNA synthesis. The addition of fresh 10% serum stimulates DNA synthesis 12 hours later with the following renewal of cell proliferation. G1-period in cells synchronized by vinblastine is 6 hours. Thus, the removal of some serum factors form cultural medium causes a reversible arresting of CHO-K1 cell proliferation.
mesh_terms: D000818:Animals; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured / Q000502:physiology*; D006224:Cricetinae; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D007106:Immune Sera / Q000494:pharmacology*; D013268:Stimulation, Chemical; D014747:Vinblastine / Q000494:pharmacology
chemical_list: D003470:Culture Media; D007106:Immune Sera; D014747:Vinblastine; D004247:DNA
vernacular_title: Proliferatsiia i sintez DNK v kul'ture kletok CHO-K1 pri razlichnoĭ kontsentratsii syvorotki v srede.