Isolation and characterization of turkey prolactin.
Prolactin from frozen turkey pituitary glands was purified by alkaline ethanol extraction of the pituitary residue after pre-extractions of the gonadotrophins and GH, followed by gel filtration on Sephadex G-100 and DEAE-cellulose column chromatography at different pH. A specific radioligand--receptor assay for ovine prolactin was used to locate the activity in eluates after column chromatography and gel filtration. The potency of the highly purified turkey prolactin was characterized by a radioligand--receptor assay to be 3.40 i.u./mg and by a heterologous radioimmunoassay to be 35 i.u./mg as compared to purified ovine prolactin as standard. The yield of turkey prolactin was 3 mg/100 g frozen pituitary glands. The purified turkey prolactin consisted of three protein bands upon polyacrylamide gel electrophoresis and the molecular weight was estimated to be 22000 by SDS--polyacrylamide gel electrophoresis. Turkey prolactin was rich in aspartic acid, glutamic acid and leucine, but lacking in methionine.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D008722:Methods; D008970:Molecular Weight; D010902:Pituitary Gland / Q000032:analysis*; D011388:Prolactin / Q000302:isolation & purification*; D011869:Radioligand Assay; D014422:Turkeys / Q000378:metabolism*
D000596:Amino Acids; D011388:Prolactin