[Modified method for producing ribonuclease A from amorphous and crystalline ribonucleases].
The paper describes a method for producing gram-scale quantities of beef pancreatic ribonuclease A (RNase A) (EC 3.1.4.22) from commercial, amorphous and crystalline RNases. The method involves enzyme purification by chromatography on SP-Sephadex G-25, rechromatography on Bio-Rax 70 followed by concentration, storage and desalting in optimal conditions. In their properties the resultant preparations of RNase A are not inferior to the best commercial samples of the enzyme.
D000818:Animals; D002417:Cattle; D003460:Crystallization; D010179:Pancreas / Q000201:enzymology*; D012260:Ribonucleases / Q000302:isolation & purification*
D012260:Ribonucleases
Modifitsirovannyĭ metod polycheniia ribonukleazya iz amorfnykh i kristallicheskikh preparatov ribonukleazy.