[DNA packing inside viral particles of Agrotis segetum (Schiff) turnip moth granulosis virus].
The structure of high molecular (88 million daltons) DNA of turnip moth granulosis virus obtained in the linear and cyclic forms was studied. Electron microscopic examination of the fine structure of DNA isolated from virus particles treated with n-toluene sulphonate-N-cyclohexyl-N'-beta-4 (methyl morpholine)-ethylcarbodiimide were carried out. Modified DNA areas were visualized by two methods: with P32 protein and phosphotungstate acid. In both cases a period of recurrence close to the length of the virus particle was revealed. This study confirmed experimentally the previously suggested model of the existence of structural disorders in DNA caused by bends of superspiral molecule at the ends of the virus particle.
D003028:CME-Carbodiimide; D004270:DNA, Circular; D004279:DNA, Viral; D007304:Insect Viruses / Q000187:drug effects / Q000648:ultrastructure*; D007915:Lepidoptera / Q000382:microbiology*; D008854:Microscopy, Electron; D008970:Molecular Weight; D009036:Moths / Q000382:microbiology*; D009690:Nucleic Acid Conformation
D004270:DNA, Circular; D004279:DNA, Viral; D003028:CME-Carbodiimide
Ob upakovke DNK vnutri virusnoĭ chastitsy virusa granuleza ozimoĭ sovki Agrotis segetum (Schiff).