Conceptus attachment in the ewe: an ultrastructural study.
Scanning and transmission electron microscopic observations were made on trophoblast, caruncles and intercaruncular areas during the attachment of the conceptus. Three stages were determined: 1. From day 14 on, precontact was established and the conceptus appeared to be immobilized in the uterine lumen. On the centres of the caruncles which were depressed and folded the epithelial cells developed bulbous cytoplasmic protrusions. Throughout the free life of the conceptus, the trophoblast cells showed an abundant covering of microvilli. 2. On day 15, apposition occurred: most microvilli on the surface of the trophoblast disappeared. 3. Between days 16 and 18, adhesion began as a result of the interpenetration of the uterine microvilli and cytoplasmic projections of the trophoblast cells. During that stage trophoblast giant cells appeared and the uterine epithelium was turned into syncytial masses; however, it was apparently not destroyed later on. Between the caruncles, the trophoblast developed finger-like villi which invaded the lumen of the uterine glands from days 15 to 18. During their short life-time (they vanish at day 20), these trophoblastic differentiations may anchor the pre-attachment conceptus and absorb the histotrophic secretions of the glands.
D000818:Animals; D002448:Cell Adhesion; D010064:Embryo Implantation; D004848:Epithelium / Q000648:ultrastructure; D005260:Female; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008871:Microvilli / Q000648:ultrastructure; D011247:Pregnancy; D012756:Sheep / Q000502:physiology*; D014327:Trophoblasts / Q000648:ultrastructure*; D014599:Uterus / Q000648:ultrastructure*