Electrochemical titrations of a ferredoxin-ferredoxin:NADP+ oxidoreductase complex.
Potentiometric titrations employing an electrochemical thin-layer cell indicate that complex formation between ferredoxin and ferredoxin:NADP+ oxidoreductase alters the midpoint oxidation-reduction potentials of both proteins. The midpoint potential of ferredoxin the complex becomes 22 +/- 6 mV more negative compared to ferredoxin alone while the midpoint potential of ferredoxin:NADP+ oxidoreductase becomes 23 +/- 4 mV more positive on complex formation.
D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism*; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010944:Plants / Q000201:enzymology*; D011199:Potentiometry / Q000379:methods; D013053:Spectrophotometry / Q000379:methods
D005288:Ferredoxins; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases