Close association of virus-specific cell surface and H-2 antigens in Ad12-infected and -transformed mouse cells.
A virus-specific cell surface (S) antigen in adenovirus type 12 (Ad12)-transformed mouse cells has been assumed to be a direct target for cytotoxic thymus derived lymphocytes (CTL). In this study, the spatial proximity between the S and H-2 antigens was determined by three different methods, the proximity and co-capping tests, and the test for blocking of CTL-mediated lysis by anti-H-2 serum. In the proximity test with Ad12-infected thymic and splenic lymphocytes, and an Ad12-transformed line of C3H-He (H-2k) mouse cells, anti-H-2k and anti-S sera reciprocally inhibited fluorescent-antibody staining of the opposite antigens. By contrast, anti-Thy-1, 2 serum as well as anti-Ia and anti-Ig sera failed to show any appreciable effect in this test, when paired with anti-S serum. In addition, the S and H-2 antigens co-capped in the infected thymic lymphocytes, and CTL-mediated lysis of the transformed cells was abrogated equally by treatment of cells with anti-S and anti-H-2 sera. These results clearly demonstrate that there is a close proximity between the S and H-2 antigens on the surface of Ad12-infected and -transformed mouse cells.
D000260:Adenoviruses, Human / Q000276:immunology / Q000502:physiology*; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002472:Cell Transformation, Viral; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000032:analysis*; D007152:Immunologic Capping; D008214:Lymphocytes / Q000382:microbiology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL
D000954:Antigens, Surface; D000956:Antigens, Viral; D006183:H-2 Antigens