Use of sorbitol in the cryopreservation of babesia.
Blood collected from mice infected with Babesia microti was frozen at two different rates to -196 degrees C using 19 per cent (w/v) glycerol as cryoprotectant. Survival of the parasites was measured by recording the parasitaemias of mice inoculated with thawed blood. Parasite survival was increased by using a solution of sorbitol in saline rather than Hanks' balanced salt solution as the diluent for the glycerol and for washing the recovered blood.
D000818:Animals; D001403:Babesia / Q000187:drug effects*; D001404:Babesiosis / Q000469:parasitology / Q000662:veterinary; D001769:Blood / Q000469:parasitology; D005615:Freezing; D008297:Male; D051379:Mice; D011309:Preservation, Biological / Q000379:methods / Q000662:veterinary*; D013012:Sorbitol / Q000494:pharmacology*
D013012:Sorbitol