Isolation of phenylalanine hydroxylase-stimulating monoclonal antibody by rat-myeloma--rat-spleen-cell fusion.
A monoclonal antibody directed against monkey liver phenylalanine hydroxylase was produced by using a rat-myeloma--rat-spleen-cell-fusion system. This antibody showed the interesting property of increasing mammalian phenylalanine hydroxylase activity more than 2-fold. Perhaps monoclonal antibodies with this effect on other enzyme or proteins could be developed.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002459:Cell Fusion; D002460:Cell Line; D004789:Enzyme Activation; D006822:Hybrid Cells / Q000276:immunology*; D009101:Multiple Myeloma / Q000473:pathology*; D010651:Phenylalanine Hydroxylase / Q000276:immunology* / Q000378:metabolism; D051381:Rats; D013154:Spleen / Q000166:cytology*
D000906:Antibodies; D010651:Phenylalanine Hydroxylase