K+, Na+, and Mg2+ content and permeability of Methanospirillum hungatei and Methanobacterium thermoautotrophicum.
The K+, Na+, and Mg2+ contents of Methanospirillum hungatei and of the thermophile Methanobacterium thermoautotrophicum were determined at various phases of growth. The intracellular K+ content of exponential phase cells of M. thermoautotrophicum (approximately 780 mM) was 5.4-fold higher than in M. hungatei, and decreased gradually as the culture entered the stationary phase. Both methanogens concentrated Mg2+, exhibiting an increased content as the cultures aged. Comparisons among extraction methods showed that most of the internal K+ was readily released, but a minimum of half of the Mg2+ in M. hungatei, and most of the M2+ in M. thermoautotrophicum, was in a bound form. Exponential phase of cells of M. hungatei established an intracellular level of Na+ lower than the outside medium, but the thermophile concentrated Na+. Dextran, inulin, sucrose, and glucose penetrated cell pellets to varying degrees and could be used to measure the space corresponding to cytoplasm and to cell wall permeability barriers. L-Phenylalanine penetrated fully and acetate accumulated in both methanogens. Acetate uptake in cell suspensions of M. hungatei was fully inhibited by oxygen. N-ethylmaleimide, or N,N'-dicyclohexylcarbodiimide, but was not affected by the proton conductor carbonylcyanide p-trifluoromethoxyphenyl-hydrazone. L-Malate, which penetrated M. hungatei cells poorly, was metabolized to glutamate, indicating the presence of an incomplete reductive carboxylic acid cycle.
D001693:Biological Transport, Active; D002453:Cell Cycle; D002463:Cell Membrane Permeability; D019605:Euryarchaeota / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
D012964:Sodium; D008274:Magnesium; D011188:Potassium