[Polyhemoglobin-based model of an artificial oxygen carrier with functional properties similar to blood].
The principal physicochemical and biochemical characteristics are described of a new artificial oxygen carrier model that represents polyhemoglobin (polyHb) with chemically attached pyridoxal-5'-phosphate (PLP), regulator of reversible hemoglobin (Hb) oxygenation. Owing to PLP attachment, the compound obtained (poly Hb-PLP) possesses the oxygen dissociation properties similar to those of blood thereby eliminating one of the main faults of exoerythrocytic Hb and of its high-molecular-weight derivatives, namely high affinity for oxygen. P50 of polyHb-PLP is shown to be 26-28 mm Hg; the oxygen dissociation curve of a mixture of blood and 12% polyHb-PLP (1 : 1) virtually coincides with the blood curve. The data are presented on autooxidation of polyHb-PLP solutions and its mixtures with plasma and blood under physiological conditions. The weight average and number average molecular masses of polyHb-PLP are 172000 +/- 16000 and 120000 +/- 11000, respectively. The width of the polydisperse model amounts to 1.4 +/0 0.3. The relative viscosity of 11-12% polyHb-PLP is about 2. The model is not toxic when injected to mice and rats in a dose of 3 g/kg bw. The evidence presented indicates that polyHb-PLP holds promise as a prospective blood substitute.
D000818:Animals; D001802:Blood Substitutes; D055598:Chemical Phenomena; D002621:Chemistry; D006454:Hemoglobins; D006863:Hydrogen-Ion Concentration; D008706:Methemoglobin; D051379:Mice; D008970:Molecular Weight; D010100:Oxygen; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives; D051381:Rats; D014783:Viscosity
D001802:Blood Substitutes; D006454:Hemoglobins; C030939:polyhemoglobin-pyridoxal-5-phosphate; D011732:Pyridoxal Phosphate; D008706:Methemoglobin; D010100:Oxygen
Model' iskusstvennogo perenoschika kisloroda na osnove poligemoglibina s funktsional'nymi svoĭstvami, blizkimi krovi.