Affinity chromatography of some pyridoxal phosphate-requiring enzymes on Cibacron Blue F3GA-agarose.
Tyrosine phenol-lyase from Erwinia herbicola was purified from a cell-free extract in a single step on Cibacron Blue F3GA-agarose. The protein was purified as the apoenzyme and was unstable after affinity chromatography. Alanine aminotransferase and aspartate aminotransferase from porcine heart also bound to Cibacron Blue F3GA-agarose. These enzymes were partially purified as holoenzymes from a crude porcine heart extract by elution with NADH and KCl. Alanine aminotransferase was purified 19 fold by this procedure.
D000410:Alanine Transaminase / Q000302:isolation & purification; D000818:Animals; D000873:Anthracenes; D001219:Aspartate Aminotransferases / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods; D004885:Erwinia / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification; D009206:Myocardium / Q000201:enzymology; D011134:Polysaccharides; D011732:Pyridoxal Phosphate / Q000378:metabolism*; D012685:Sepharose / Q000031:analogs & derivatives; D013552:Swine; D014447:Tyrosine Phenol-Lyase / Q000302:isolation & purification
D000873:Anthracenes; D011134:Polysaccharides; C017842:cibacron blue-sepharose; D011732:Pyridoxal Phosphate; D012685:Sepharose; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D014447:Tyrosine Phenol-Lyase