A laser Raman spectroscopic study of Ca2+ binding to troponin C.
Laser Raman spectroscopy has been used detect structural changes in troponin C induced by Ca2+ binding. Addition of Ca2+ - Mg2+ sites produces perturbations in the amide III region of the spectrum indicative of increased alpha-helical content, and in regions of the spectrum corresponding to carboxylate, thiol, and phenol side chains. However, Ca2+ binding to the low affinity Ca2+ - specific sites is not detected by laser Raman spectral changes.
D000818:Animals; D002118:Calcium; D007834:Lasers; D008274:Magnesium; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles; D011485:Protein Binding; D011487:Protein Conformation; D011817:Rabbits; D013059:Spectrum Analysis, Raman; D014336:Troponin / Q000378:metabolism*; D019209:Troponin C
D009124:Muscle Proteins; D014336:Troponin; D019209:Troponin C; D008274:Magnesium; D002118:Calcium