[Plant antigens with immunological properties. III. Separation of a biologically active fraction in a grass pollen extract].
In present paper a partial biochemical study concerning two grass pollens (Poa pratensis and Phleum pratense) is carried out. On Sephadex G-75 three fractions were detected by an extract from the grass pollens. Only the third fraction shows a biological activity and biochemical findings are in agreement with the presence of four polipeptidic subfractions of different molecular weights ranged between 10,000 and 14,500. Specific additionals trials (ultracentrifugation, disc-electrophoresis, thyn-layer electrophoresis, isoelectric-focusing, immunological studies) are carried out to confirm the observed nature of prevalent material in pollens.
D000941:Antigens / Q000302:isolation & purification; D010936:Plant Extracts / Q000276:immunology*; D006109:Poaceae; D011058:Pollen / Q000276:immunology*
D000941:Antigens; D010936:Plant Extracts
Antigeni vegetali dotati di proprieta' immunologiche. III. Separazione di una frazione biologicamente attiva in un estratto di pollini di graminacee.