Rapid microanalysis of anticonvulsants by high-performance thin-layer chromatography.
A rapid microanalytical method is described for phenobarbital, phenytoin, primidone and carbamazepine utilizing high-performance thin-layer chromatography (HPTLC). This procedure incorporates a single extraction of a 50-microliter plasma sample. One tenth of the extract is concentrated and applied to the HPTLC plate by a Contact Spotter, chromatographically separated and quantitated by in situ ultraviolet reflectance densitometry. The coefficient of variation is less than 4% (n = 8), the extraction efficiency is approximately 95% and the minimum detectable amount of pure drug standards applied to and developed on the HPTLC plate is 5 ng or less for all four anticonvulsants.
D000927:Anticonvulsants / Q000097:blood*; D002220:Carbamazepine / Q000097:blood; D002855:Chromatography, Thin Layer / Q000379:methods; D006801:Humans; D010634:Phenobarbital / Q000097:blood; D010672:Phenytoin / Q000097:blood; D011324:Primidone / Q000097:blood; D012016:Reference Values
D000927:Anticonvulsants; D011324:Primidone; D002220:Carbamazepine; D010672:Phenytoin; D010634:Phenobarbital