Increased biliary GSSG-secretion and loss of hepatic glutathione in isolated perfused rat liver after paraquat treatment.
Perfusion of isolated rat livers with 1 mM paraquat for 3 hours led to a stimulated release of oxidized glutathione into the effluent caval perfusate and into the bile. Whereas the biliary stimulation was 245%, stimulation in to the perfusate was only 19.2%. In addition, the glutathione content in the paraquat-treated livers decreased from 2.74 +/- 0.23 to 0.80 +/- 0.07 mumol/g liver. The hepatic content of GSSG, however, was not changed by paraquat. This resulted in an elevated ratio of GSSG/GSH+2GSSG from 0.036 to 0.113. The total amount of GSSG released via bile and perfusate is less than the total loss of hepatic GSH. These findings are discussed in view of mechanisms by which paraquat decreases in GSH in the liver.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D010269:Paraquat / Q000633:toxicity*; D010477:Perfusion; D051381:Rats
D005978:Glutathione; D010269:Paraquat