ADP-arsenate. Formation by submitochondrial particles under phosphorylating conditions.
Submitochondrial particles from beef heart mitochondria synthesize ADP-arsenate from ADP and arsenate when energized by succinate. The ADP-arsenate formed hydrolyzes rapidly and this is almost certainly the mechanism by which arsenate "uncouples" oxidative phosphorylation. When sufficient hexokinase is present, a substantial portion of the ADP-arsenate formed reacts with glucose to form glucose 6-arsenate and ADP. The glucose 6-arsenate thus formed hydrolyzes, at pH 7.5 and 30 degrees C, under the conditions used, with a rate constant of 5.5 X 10(-4) s-1 and is a substrate for glucose-6-phosphate dehydrogenase.
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001149:Arsenates / Q000378:metabolism*; D001151:Arsenic / Q000378:metabolism*; D002417:Cattle; D005947:Glucose / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D013367:Submitochondrial Particles / Q000187:drug effects / Q000378:metabolism*; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid
D001149:Arsenates; D010710:Phosphates; D013386:Succinates; D009249:NADP; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D019802:Succinic Acid; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D005947:Glucose; D001151:Arsenic; C025657:arsenic acid