Highly purified ceruloplasmin messenger RNA from rat liver. Physico-chemical and functional characteristics.
Highly purified ceruloplasmin mRNA was isolated from rat liver polyribosomes. The molecular weight of ceruloplasmin mRNA is in a range from 1.05 to 1.25 . 10(6) daltons which is large enough to code for a putative precursor of ceruloplasmin (approximately 700 amino acid acids). Ceruloplasmin mRNA contains 3'-terminal poly(A) the length of which varies from 38 to 165 nucleotides. The 5'-end of ceruloplasmin mRNA is blocked with confronting m7G residue which is a component of cap I (m7G5'ppp5'XmpAp). The addition of ceruloplasmin mRNA to wheat-germ cell free system programmed the synthesis of a product that was largely precipitated by anti-ceruloplasmin immunoglobulins. The translation product was homogeneous in polyacrylamide gel-sodium dodecylsulfate electrophoresis. Cell-free translation of ceruloplasmin mRNA was sensitive to inhibition by cap analogue.
D000818:Animals; D002570:Ceruloplasmin / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis*; D009690:Nucleic Acid Conformation; D010527:Perissodactyla; D011061:Poly A / Q000032:analysis; D011132:Polyribosomes / Q000032:analysis*; D012333:RNA, Messenger / Q000302:isolation & purification*; D011817:Rabbits; D051381:Rats
D012333:RNA, Messenger; D011061:Poly A; D002570:Ceruloplasmin