High resolution gas chromatography mass spectrometry of the methyl esters of organic acids from uremic hemofiltrates.
The organic acid fraction of hemofiltrates was investigated in the form of methylates by glass capillary gas chromatography-mass spectrometry. The pattern obtained is similar to that of urinary organic acid methylates from healthy individuals. A marked difference was noted for N-phenylacetyl-alpha-aminoglutarimide, present in hemofiltrate at levels 50-100 times higher than those in urine. Analysis of hemofiltrate samples taken at different times during a hemofiltration with post-dilution technique revealed that the hemofiltrate concentration of most compounds was drastically reduced the course of the hemofiltration treatment. Compared to the other compounds, the reduction in hemofiltrate concentration of N-phenylacetyl-alpha-aminoglutarimide was extremely rapid.
D000328:Adult; D001769:Blood; D002264:Carboxylic Acids / Q000097:blood*; D004952:Esters / Q000097:blood* / Q000652:urine; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D014462:Ultrafiltration; D014511:Uremia / Q000097:blood* / Q000652:urine
D002264:Carboxylic Acids; D004952:Esters