X-irradiation and the incorporation of precursors for salvage and de novo synthesis of DNA in hepatomas and liver.
The effect of X-irradiation 15 Gy (1500 rad) on precursor incorporation into DNA was studied in transplanted Morris hepatomas and liver of the tumor-bearing rats. Precursors were studied which are utilized by salvage mechanisms ([3H] thymidine and [3H]deoxyuridine) or in de novo synthesis of DNA ([14C]aspartate and [14C]formate). The data suggested that salvage mechanisms are more sensitive to inhibition by X-irradiation and that DNA repair activity is greater in liver than hepatomas.
D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D004247:DNA / Q000528:radiation effects; D004260:DNA Repair; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008297:Male; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D014498:Uracil / Q000378:metabolism; D014965:X-Rays
D004273:DNA, Neoplasm; D001224:Aspartic Acid; D014498:Uracil; D004247:DNA; D013936:Thymidine