Metabolism of 3,3'-Diiodothyronine and 3'-monoiodothyronine, and effect of potassium cyanide and dinitrophenol and glucuronidation of thyroxine in cultured rat hepatoma cells.
Rat hepatoma cells (R117-21B) metabolized 3,3'diiodothyronine and 3'-monoiodothyronine by sulfation and glucuronidation. Glucuronidation of iodothyronines (a mixture of 3,3'-diiodothyronine, approximately 99%, and 3'-monoiodothyronine, approximately 10%(was very rapid and 83% of the total 125I in the medium was their glucuronides after 8 hr incubation with the cells at 37 degrees C. Iodothyronine sulfates (approximately 9% of the total 125I) were also detected. No clear increase in free iodide was observed during the 8 hr incubation. The effect of KCN or dinitrophenol on glucuronidation of thyroxine was investigated. Both drugs inhibited glucuronidation, and dinitrophenol was more effective than KCN. Dinitrophenol accelerated the liberation of 125I- in the metabolism of thyroxine in the cultured rat hepatoma cells.
D000818:Animals; D002478:Cells, Cultured; D003486:Cyanides / Q000494:pharmacology*; D004104:Diiodothyronines / Q000378:metabolism*; D004140:Dinitrophenols / Q000494:pharmacology*; D005965:Glucuronates / Q000378:metabolism; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D011190:Potassium Cyanide / Q000494:pharmacology*; D051381:Rats; D013970:Thyronines / Q000378:metabolism*; D013974:Thyroxine / Q000378:metabolism*
D003486:Cyanides; D004104:Diiodothyronines; D004140:Dinitrophenols; D005965:Glucuronates; D013970:Thyronines; C019017:3'-monoiodothyronine; D011190:Potassium Cyanide; D013974:Thyroxine