[Myelinotoxic activity of sera from animals with experimental allergic encephalomyelitis in organotypic cultures of the rat cerebellum at different stages of differentiation].
Radiometry was used to assay myelinization and demyelinization in organotypic long-surviving cultures of the cerebellum from newborn rats. It was shown that serum from guinea-pigs with allergic encephalomyelitis suppresses by 45% the incorporation of labeled cholesterol into nerve tissue cells in the early periods of cultivation (within 10 days), i.e. before myelinization commencement. It also suppresses thymidine and cholesterol incorporation in the later periods of cultivation, i.e. in the last stages of differentiation, this suppression being increased twofold in the myelinized cultures of the nerve tissue. The effect of humoral factors of the sera from sick animals on the neuroglial cells in the course of nerve tissue differentiation in vitro is discussed.
D000818:Animals; D002454:Cell Differentiation; D002525:Cerebellar Cortex / Q000254:growth & development / Q000276:immunology*; D046508:Culture Techniques; D003602:Cytotoxicity, Immunologic; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D006168:Guinea Pigs; D007106:Immune Sera / Q000494:pharmacology*; D009186:Myelin Sheath / Q000276:immunology*; D009457:Neuroglia / Q000276:immunology; D009928:Organ Specificity; D051381:Rats
D007106:Immune Sera
Mielinotoksicheskaia aktivnost' syvorotok zhivotnykh s éksperimental'nym allergicheskim éntsefalomielitom v organotipicheskikh kul'turakh kory mozzhechka krys na raznykh étapakh ikh differentsirovki.