title: Gene mapping of human ocular connective tissue proteins. Assignment of the structural gene for corneal type I procollagen to human chromosome 7 in human corneal stroma-mouse fibroblast somatic cell hybrids.
abstract: Somatic cell hybrids between mouse and human corneal stroma fibroblasts have been used to identify the human chromosome responsible for the synthesis of human corneal type I procollagen. Twenty-six separate hybrid clones and subclones from three separate hybridization events were isolated in hypoxanthine-aminopterin-thymidine (HAT) selection medium and were used to assay for the production of human type I procollagen. Human and mouse chromosomes were identified in each hybrid clone by alkaline Giemsa staining of metaphase chromosomes spread and by isozyme analysis. We have found that human type I procollagen production segregates concordantly with human chromosome 7 and with beta-glucuronidase, another human chromosome 7 marker. The pro-alpha 1 gene and possibly the pro-alpha 2 gene appear to be encoded on human chromosome 7. Because we have previously assigned the gene for human skin type I procollagen to chromosome 17, our present data indicate that separate genes and control mechanisms must exist for skin and corneal type I procollagen.
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D003238:Connective Tissue; D003315:Cornea / Q000502:physiology; D005136:Eye Proteins / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D066298:In Vitro Techniques; D051379:Mice; D011347:Procollagen / Q000235:genetics; D012879:Skin Physiological Phenomena
chemical_list: D005136:Eye Proteins; D011347:Procollagen