Determination of the relative amounts of the B and C components of neomycin by ion-exclusion chromatography using refractometric detection.
Refractometric detection can be used as a convenient alternative to ninhydrin colorimetric or polarimetric detection in ion-exclusion chromatography of neomycinn. The determination of the relative amounts of neomyci B and C using different detection methods is examined. The use of a resin of smaller granulometry and a medium-pressure chromatographic apparatus reduces the analysis time to less than 25 min.
D002850:Chromatography, Gel / Q000379:methods; D005601:Framycetin / Q000302:isolation & purification*; D007536:Isomerism; D009355:Neomycin / Q000032:analysis*; D012031:Refractometry; D013678:Technology, Pharmaceutical
D005601:Framycetin; D009355:Neomycin