Studies on the inactivation of glyceraldehyde-3-phosphate dehydrogenase by methylglyoxal.
Glyceraldehyde-3-P dehydrogenase (E.C. 1.2.1.12) from rabbit muscle is rapidly inactivated by methylglyoxal following pseudo first-order kinetics. Substrate, as well as inorganic phosphate, affords protection, whereas NAD+ is ineffective. The arginine residue implicated in the reaction is probably the anion binding site of the phosphate group of the substrate.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D001120:Arginine / Q000032:analysis; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000037:antagonists & inhibitors*; D007700:Kinetics; D009132:Muscles / Q000201:enzymology; D009243:NAD / Q000494:pharmacology; D011485:Protein Binding; D011765:Pyruvaldehyde / Q000494:pharmacology*; D011817:Rabbits
D000447:Aldehydes; D009243:NAD; D011765:Pyruvaldehyde; D001120:Arginine; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases