Neutrophil-mediated cytotoxicity to schistosomula of Schistosoma mansoni in vitro: studies on the kinetics of complement and/or antibody-dependent adherence and killing.
The capacity of rat peritoneal neutrophils to adhere to and kill schistosomula of Schistosoma mansoni in vitro has been investigated. Neutrophils adhere readily to schistosomula in the presence of antibody plus complement (C) and C alone (fresh normal rat serum), but not with heat-inactivated normal rat serum. However, schitosomular killing is only achieved with neutrophils and fIRS or fNRS. In the presence of hiIRS the cells detach after 6 h without producing a significant level of parasite death. The system involving neutrophils plus fIRS is the most efficient in terms of serum dilution and the rate of schistosomular killing. The complement-dependent antibody involved in this system belongs to the class IgG and occurs in rat serum at peak titres, 6-8 wk after a primary schistosome infection. Neutrophil adherence in the presence of fNRS depends upon the generation of C3b molecules at the parasite surface via the alternative pathway of C activation. Studies on the antibody alone system indicate that the lack of significant schistosomular killing might result from the absence of factors which stimulate cell migration, since if a chemokinetic agent is introduced into the assay a 30% increase in mortality is recorded. The possible participation of neutrophils in the destruction of a primary and/or challenge infection in vivo is discussed.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D003165:Complement System Proteins / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D007104:Immune Adherence Reaction; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D009504:Neutrophils / Q000276:immunology*; D012550:Schistosoma mansoni / Q000254:growth & development*; D013997:Time Factors
D003165:Complement System Proteins