K+-evoked Caa+-dependent release of D-[3H]aspartate from terminals of the cortico-pontine pathway.
Pontine nuclei dissected from rat brain slices released previously accumulated D-[3H]aspartate (D-Asp) and [14C]gamma-aminobutyrate (GABA) Ca-dependently when exposed to 50 mM K. These efflux rates were substantially increased by including 0.5 mg/ml bovine serum albumin in the superfusion fluid. Degeneration of the cortico-pontine fibres 5 days after cutting the crus cerebri caused an 80% reduction in the fractional rate of Ca-dependent D-Asp release and a 60% reduction in uptake. The fractional rate of GABA efflux was significantly less reduced than that of D-Asp efflux, and GABA uptake was nearly unchanged.
D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000378:metabolism*; D004525:Efferent Pathways / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D011149:Pons / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology*; D051381:Rats; D014316:Tritium; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D014316:Tritium; D001224:Aspartic Acid; D005680:gamma-Aminobutyric Acid; D011188:Potassium; D002118:Calcium