Blastogenic response of swine lymphocytes in whole blood, purified blood, spleen cell, and lymph node cell cultures with concanavalin A and phytohemagglutinin.
Microcultures of swine whole blood, purified blood lymphocytes (PBL), spleen cells, and lymph node (LN) cells were tested for stimulation by concanavalin A (conA) and phytohemagglutinin (PHA). A cell concentration of 2 x 10(6)/ml and a 1:20 dilution of whole blood were needed for optimal stimulation of conA and PHA. Optimal stimulation of whole blood lymphocytes (WBL) and LN cells was obtained by 20 micrograms of conA/ml, but 10 micrograms/ml of this mitogen was adequate for spleen cells. The PBL and LN cells were optimally stimulated by 20 microliters of PHA/ml, but 80 microliters and 40 microliters/ml of this mitogen were required for stimulating the whole blood and spleen cells, respectively. Four days' incubation was needed for maximal blastogenesis of WBL with conA. This could be achieved after 3 days' incubation with PBL and spleen and LN cells. Whole blood and spleen cells were stimulated by PHA after 3 days' incubation, but PBL and LN cells required 4 days' incubation. Reproducibility, sensitivity, and comparison of various cultures were discussed.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D008198:Lymph Nodes; D008214:Lymphocytes / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013552:Swine / Q000097:blood*
D010835:Phytohemagglutinins; D003208:Concanavalin A