title: The effects of substrate flow-rate on immobilized urease assays.
abstract: Urease (urea aminohydrolase, EC 3.5.1.5) has been immobilized on o-alkylated nylon tubes in an enzymatically active form. The measured activity of the enzyme coil has been shown to be dependent on the flow rate of substrate through it. The apparent Km decreased and V increased as flow rates were increased. It is suggested that diffusional control is responsible for these observations. Their implications in flow-through immobilized enzyme analyses are discussed.
mesh_terms: D004800:Enzymes, Immobilized / Q000378:metabolism*; D007700:Kinetics; D009757:Nylons; D012212:Rheology; D014510:Urease / Q000378:metabolism*
chemical_list: D004800:Enzymes, Immobilized; D009757:Nylons; D014510:Urease