Sickled erythrocytes accelerate clotting in vitro: an effect of abnormal membrane lipid asymmetry.
A membrane lipid abnormality induced by sickling and found as a permanent alteration in the irreversibly sickled cell (ISC) is the rearrangement of phosphatidyl ethanolamine (PE) and phosphatidyl serine (PS) from the inner to the exterior side of the lipid bilayer. Since PS can provide a catalytic surface for the binding of blood coagulation factors and thus can exhibit procoagulant activity, we investigated the influence of oxy and deoxy reversibly sickled cells (RSC) ass well as ISC on clotting in vitro. Red blood cells (RBC), as the source of phospholipid, were added to platelet-poor citrated plasma containing Russell's viper venom (RVV) and clotting time was measured after recalcification. The clotting time after addition of normal RBC and oxy-RSC was similar to the saline blank (100 sec). In contrast, both oxy-ISC and deoxy completely sickled RSC shortened clotting time by 30%. Using liposomes prepared with identical phospholipid composition to the outer lipid leaflet of either normal RBC, RSC or ISC clotting times similar to those with intact cells were achieved. Since the liposomes did not contain protein, accentuation of clotting appears to be related to abnormal phospholipid organization, in particular to the abnormal exposure to aminophospholipids on the outer surface of the membrane. This abnormality may contribute to the pathogenesis of the vaso-occlusive episode in sickle cell anemia.
D000755:Anemia, Sickle Cell / Q000097:blood*; D001777:Blood Coagulation; D004910:Erythrocyte Membrane / Q000473:pathology*; D004912:Erythrocytes / Q000473:pathology*; D006801:Humans; D008081:Liposomes / Q000494:pharmacology; D008563:Membrane Lipids; D010100:Oxygen / Q000378:metabolism; D011517:Prothrombin Time
D008081:Liposomes; D008563:Membrane Lipids; D010100:Oxygen