[Electron microscope characteristics of the nuclear matrix and its fractions].
The rat liver nuclear matrix retains the shape of the nucleus and reveals a sponge-like structure in negative staining and scanning electron microscopy. A fibrous layer (dense lamina) with associated pore complexes are preserved on the surface of the nuclear matrix. The cytoplasmic face of the nuclear matrix is perceived as a network consisting of cells (or units) of 10-30 nm in diameter in negative staining as well as in high resolution scanning electron microscopy. In sections, a fibrous layer, 15-30 nm in width with granules of 7-10 nm in diameter, can be observed. In pore complexes associated with the fibrous layer granular and fibrillar components rather than central granules are observed. The pore complexes differ in arrangement of the annular granules. Structures similar to pore complexes are revealed in close proximity to the nucleoli. The biogenesis of the pore complexes is discussed. A few morphologically different structures could be derived be fractionation of the nuclear matrix. A fraction rich in pore complexes, and a fraction retaining the shape of the nucleus with spongy or alveolar structure were isolated. The latter fraction is regarded to form a protein framework or skeleton of the nucleus.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D002469:Cell Separation; D008099:Liver / Q000648:ultrastructure; D008297:Male; D008855:Microscopy, Electron, Scanning; D009685:Nuclear Envelope / Q000648:ultrastructure; D051381:Rats
Elektronno-mikroskopicheskaia kharakteristika iadernogo matriksa i ego fraktsii.