Purification of malic enzyme from Ascaris suum using NAD+-agarose.
Malic enzyme has been purified from Ascaris suum by polyethylene glycol precipitation, ion-exchange chromatography, ammonium sulfate precipitation, and NAD-agarose affinity chromatography to a specific activity of 80 units/mg (V/[E]t = 350 s-1). The preparation was shown to be homogeneous by SDS polyacrylamide disc gel electrophoresis. The procedure can be accomplished in a maximum of four days with a 74% yield.
D001200:Ascaris / Q000201:enzymology*; D002846:Chromatography, Affinity; D008291:Malate Dehydrogenase / Q000302:isolation & purification*; D011134:Polysaccharides / Q000494:pharmacology*; D012685:Sepharose / Q000031:analogs & derivatives / Q000494:pharmacology*
C030573:NAD-agarose; D011134:Polysaccharides; D012685:Sepharose; D008291:Malate Dehydrogenase; C030574:malate dehydrogenase (decarboxylating)