Evaluation of the new Beckman electrophoretic method for CK-MB: comparison with ion exchange chromatography and investigation of a reference outpatient population.
We evaluated the performance of a CK isoenzyme kit utilizing agarose electrophoresis with fluorescence scanning of an overlay paper rather than the gel. We compared this method to ion exchange chromatography with mini-columns of DEAE Sephadex using samples from hospitalized patients and from a reference population of ambulatory outpatients with common, chronic medical conditions. The detection limit, precision and linearity of the electrophoretic method were acceptable, but the method overestimated CK-MB, requiring a different reference range for clinical application. There were inter-racial differences within the reference population for total CK and for the percent of CK-MB using either method. We discuss the significance of our findings with respect to clinical decision limits for CK-MB.
D000328:Adult; D000368:Aged; D044383:Black People; D002852:Chromatography, Ion Exchange; D003327:Coronary Disease / Q000201:enzymology; D003402:Creatine Kinase / Q000097:blood*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005260:Female; D006801:Humans; D007527:Isoenzymes; D008297:Male; D008875:Middle Aged; D013696:Temperature; D044465:White People
D007527:Isoenzymes; D003402:Creatine Kinase