Dynamic cation-exchange systems for rapid separations of nucleobases and nucleosides by high-performance liquid chromatography.
The retention behaviour of nucleobases and nucleosides in dynamic cation-exchange systems, consisting of a hydrophobic support as the stationary phase and water-ethanol mixtures containing small amounts of sodium dodecylsulphate as anionic detergent as the mobile phase was investigated. The retention of nucleobases and nucleosides can be influenced over a wide range by variation of the pH and the concentration of the ethanol, anionic detergent and counter ion in the eluent. With respect to separation speed and selectivity, these dynamic cation-exchange systems are in many instances superior to conventional ion-exchange and reversed-phase systems. It is shown that, by optimizing the different retention parameters, the separation of fourteen nucleobases and nucleosides, simultaneously and under isocratic conditions, can be achieved in ca. 6 min. The performance of the phase system is demonstrated by the analysis of a calf thymus DNA hydrolysate.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009705:Nucleosides / Q000032:analysis*; D011687:Purines / Q000032:analysis; D011743:Pyrimidines / Q000032:analysis; D013950:Thymus Gland / Q000032:analysis*
D009705:Nucleosides; D011687:Purines; D011743:Pyrimidines