[Assay for detection of ciguatoxin using an immunoenzyme technic].
The ELISA method has been used to detect possible anticiguatoxin antibodies in the tissues of toxic fishes. Thin lamellae of toxic fish muscles have been brought into contact on the one hand with antibodies obtained after immunization of the rabbit and the mouse by a human serumalbumine ciguatoxin conjugate and on the other hand with human sera of ciguatera convalescent. These various antibodies have been recognized by peroxydase labelled anti-rabbit, -mouse and -human antibodies. It has been not possible to show off specific antibodies of the ciguatoxin in the various immune sera tested by this immuno-enzymatic method.
D000818:Animals; D000992:Antitoxins / Q000276:immunology; D002922:Ciguatoxins / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D005400:Fishes, Poisonous; D006801:Humans; D008387:Marine Toxins / Q000032:analysis*; D051379:Mice; D011817:Rabbits
D000992:Antitoxins; D008387:Marine Toxins; D002922:Ciguatoxins
Essai de détection de la ciguatoxine par une méthode immunoenzymatique.