Photographic defocusing: a means to render recognizable line traced models of negatively stained biological specimens.
A simple method of obtaining analogue images from traced models of biological specimens is presented. It consists of the photographic defocusing of traced models and it is illustrated with negatively stained cylindrical forms of the ASFV; the black lines of the trace in the model correspond to the negative stain surrounding the viral morphological subunits as seen in the electron micrograph. The photographic defocusing is the means by which the traced model is filtered and is used to introduce grey levels on an otherwise black and white image. The right amount of defocusing is attained when the width of the trace of the model equals the width of the rim of the negative stain appearing between the morphological subunits in the electron micrograph.
D000358:African Swine Fever Virus / Q000648:ultrastructure; D000818:Animals; D003201:Computers; D008854:Microscopy, Electron; D008961:Models, Structural; D055095:Optics and Photonics; D010784:Photomicrography / Q000379:methods*