Identification of hydrazone of norethindrone, a metabolic product resulting from the interaction between isoniazid and norethindrone.
Co-administration of isonicotinic acid hydrazide (isoniazid, INH) and 17 alpha-ethinyl-17 beta-hydroxyestr-4-en-3-one (norethindrone, NE) resulted in the formation of the isonicotinyl hydrazone of norethindrone (INH-NE) in rat stomach. Rat liver metabolized the latter compound in vitro. The metabolic product was characterized, following its derivatization with p-methoxy-benzaldehyde (PMBA), by comparison of chromatographic and mass spectral properties with synthetic reference compound. Results showed that INH-NE was cleaved at the amide bond resulting in the formation of the hydrazone of norethindrone. The physicochemical characteristics of synthetic PMBA hydrazone of norethindrone are described.
D000818:Animals; D004347:Drug Interactions; D005260:Female; D006835:Hydrazones / Q000302:isolation & purification*; D007538:Isoniazid / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D009640:Norethindrone / Q000378:metabolism*; D051381:Rats
D006835:Hydrazones; D009640:Norethindrone; D007538:Isoniazid