On the specificity of uptake by isolated Torpedo synaptic vesicles.
Synaptic vesicles were isolated from the electric organ of Torpedo marmorata in highly purified form. Their uptake properties were examined using a large number of small organic molecules as substrates. Following incubation at 26 degree C for 1 h, it was found that concentrative accumulation, indicated by a vesicle:medium concentration ratio greater than unity, was achieved by all the choline analogues used and by four biogenic amines, but not by a variety of purine and pyrimidine bases and nucleosides. Amino acids penetrated poorly, as did sugars, and of organic anions, acetate but not citrate or thiocyanate, was almost excluded. Thus Torpedo vesicles are relatively impermeable to compounds which cannot utilize the ACh or ATP carriers, but show a very high rate of amine uptake, which may be linked to a pH gradient.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism; D001692:Biological Transport; D002794:Choline / Q000031:analogs & derivatives / Q000378:metabolism; D004557:Electric Organ / Q000378:metabolism*; D005399:Fishes; D007700:Kinetics; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D011687:Purines / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D012263:Ribonucleosides / Q000378:metabolism; D013329:Structure-Activity Relationship; D013572:Synaptic Vesicles / Q000378:metabolism*
D000596:Amino Acids; D001679:Biogenic Amines; D004364:Pharmaceutical Preparations; D011687:Purines; D011743:Pyrimidines; D012263:Ribonucleosides; D002794:Choline