Polyribosome analysis on sucrose gradients produced by the freeze-thaw method.
Polyribosomes were analyzed on sucrose gradients produced by the freeze-thaw method. When gradients were formed from a starting solution containing 1 mM Mg2+ an anomalous sedimentation pattern was observed although the polyribosomal structure can be maintained at this Mg2+ concentration. The use of starting solutions containing 5--20 mM Mg2+ yielded a satisfactory sedimentation profile of polyribosomes on freeze-thaw produced gradients.
D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D005615:Freezing; D008274:Magnesium; D010944:Plants / Q000648:ultrastructure; D011132:Polyribosomes / Q000648:ultrastructure*
D008274:Magnesium