Radioimmunoassay for detection of anti-oligodendrocyte antibodies.
A solid phase radioimmunoassay (RIA) for detection and quantitation of rabbit anti-oligodendrocyte antibody has been developed using bovine oligodendroglia preparation. The assay is simple, rapid, reproducible and economical. It is approximately 150 x as sensitive as immunofluorescence. Specificity has been established by using different bovine tissue antigens as substrate and absorption studies. This assay represents a potentially powerful tool for the detection and quantitation of anti-oligodendroglial antibodies and oligodendroglial antigens in serum and CSF of man and experimental animals.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002417:Cattle; D009457:Neuroglia / Q000276:immunology*; D009836:Oligodendroglia / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D000906:Antibodies; D012710:Serum Albumin, Bovine