[Electrophoretic study of Potato tuber plasmolemmal proteins].
The number and qualitative content of proteins extracted from potato tuber plasmolemma by buffer solutions of various ionic strengths and the non-ionic detergent Triton X-100 were investigated. It was shown that treatment with the buffer solutions of various ionic strengths results in separation of various amounts of proteins from the membranes. The proteins extracted at various ionic strengths differ in their electrophoretic mobility and the lipo- and glycoprotein sets. The extraction of the bulk of peripheral proteins by buffer solutions does not affect the vesicular structure and the three-layer structure of the vesicles in the plasmolemmal preparation. On the contrary, Triton X-100 has a destructive effect on the plasmolemmal vesicles. The amount of protein extracted with Triton X-100 is relatively low and does not increase with the increase in the detergent concentration from 1% up to 10%. Pretreatment of the preparation with buffer solutions decreases the amount of protein extracted by Triton X-100 irrespective of the ionic strength of the buffer. The electrophoregrams of the proteins extracted by the buffer solutions and Triton X-100 revealed that the detergent can dissolve both structural and peripheral proteins of the plasmolemma. Treatment of plasmolemma with buffer solutions causes separation of hydrophylic proteins from the membranes and brings about changes in the properties of the membrane-bound proteins.
D002021:Buffers; D002462:Cell Membrane / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis; D008074:Lipoproteins / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D017830:Octoxynol; D009994:Osmolar Concentration; D010940:Plant Proteins / Q000032:analysis*; D011092:Polyethylene Glycols
D002021:Buffers; D006023:Glycoproteins; D008074:Lipoproteins; D008565:Membrane Proteins; D010940:Plant Proteins; D011092:Polyethylene Glycols; D017830:Octoxynol
Elektroforeticheskoe issledovanie belkov plazmalemmy klubneĭ kartofelia.