Simplified method for synthesizing ampholytes suitable for use in isoelectric focusing of immunoglobulins in agarose gels.
D000180:Acrylic Resins; D000665:Ampholyte Mixtures / Q000138:chemical synthesis*; D000818:Animals; D002021:Buffers / Q000138:chemical synthesis*; D005782:Gels; D007136:Immunoglobulins / Q000302:isolation & purification*; D007525:Isoelectric Focusing / Q000379:methods*; D011073:Polyamines; D011817:Rabbits; D012685:Sepharose
D000180:Acrylic Resins; D000665:Ampholyte Mixtures; D002021:Buffers; D005782:Gels; D007136:Immunoglobulins; D011073:Polyamines; C016680:polyacrylamide gels; D012685:Sepharose; C031122:pentaethylenehexamine