Stimulatory effect on DNA synthesis of thymus and spleen extract from leukemic AKR mice.
A 30,000-50,000 molecular weight (MW) extract of thymus and spleen from 11 age groups of untreated AKR mice, 1-210 days old, was tested for in vitro effect on thymidine incorporation into normal AKR lymph node cells, normal spleen cells, and leukemic thymocytes. Extracts from mice up to 5 months of age mostly had slightly inhibitory effect but concomitant with emergence of thymus leukemia in the 6-month-old mice, the extract acquired a strongly mitogenic effect on normal lymph node cells. Seven-month-old, non-leukemic animals again yielded extracts with inhibitory effect.
D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D008806:Mice, Inbred AKR; D008970:Molecular Weight; D013154:Spleen / Q000502:physiology*; D013268:Stimulation, Chemical; D013949:Thymus Extracts / Q000494:pharmacology*; D014020:Tissue Extracts / Q000494:pharmacology*
D013949:Thymus Extracts; D014020:Tissue Extracts; D004247:DNA