Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against the H-Y antigen on murine sperm.
An enzyme-linked immunosorbent assay (ELISA) using murine spermatozoa and thymocytes is described for the detection of anti-H-Y antibodies. ELISA is more sensitive and reproducible than cytotoxicity and the PA-SRBC test. It is particularly useful for the rapid screening of a large number of samples.
D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D000941:Antigens; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007106:Immune Sera / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011817:Rabbits; D012756:Sheep; D013094:Spermatozoa / Q000276:immunology*; D013205:Staphylococcal Protein A / Q000494:pharmacology
D000906:Antibodies; D000941:Antigens; D007106:Immune Sera; D013205:Staphylococcal Protein A