Determination of indoles and catechols in rat brain and pineal using liquid chromatography with fluorometric and amperometric detection.
Tryptophan, serotonin, 5-hydroxyindoleacetic acid, and homovanillic acid were determined in rat brain by the direct injection of a centrifuged tissue homogenate into a liquid chromatographic-fluorometric/amperometric system. The above indoles, along with melatonin, were also determined in single rat pineal glands. The utility of the system in determining several additional catechols and idoles in brain was examined.
D000818:Animals; D001923:Brain Chemistry; D002396:Catechols / Q000032:analysis*; D002853:Chromatography, Liquid; D007211:Indoles / Q000032:analysis*; D010870:Pineal Gland / Q000032:analysis*; D051381:Rats; D012016:Reference Values
D002396:Catechols; D007211:Indoles