[Quantitative determination of total urinary protein utilizing the principle of Coomassie Brilliant Blue G250 binding to protein (author's transl)].
Coomassie Brilliant Blue G250 was used for the determination of total urinary protein and compared with the biuret-procedure. In the Coomassie Brilliant Blue G250-assay 0.10 ml urine are added to 5.0 ml Coomassie Brilliant Blue G250 reagent and the sample is read against the reagent blank. The Coomassie Brilliant Blue G250 method seems to be superior to the biuret-procedure because of better reproducibility, higher sensitivity and simpler handling. The upper limit or urinary protein excretion in 49 healthy subjects was 120 mg/24 h. To evaluate the clinical significance of the Coomassie Brilliant Blue G250 method the urinary protein concentration of 134 patients with metabolic, systemic and organ diseases was compared with the biuret-procedure. The regression line was y = 0.827 X + 8.713 mg/l with a correlation coefficient of r = 0.966. The type of proteinuria (mixed, glomerular, tubular) had no influence on the protein value measured with the Coomassie Brilliant Blue G250 method. However in selective proteinuria, like Bence-Jones protein excretion, there is no correlation between the two methods, because the concentration of Bence-Jones protein is underestimated. Lower protein values were also frequently obtained for patients with diabetes mellitus. Some specimens from patients with chronic renal failure treated by haemodialysis showed elevated values. In spite of these limitations the Coomassie Brilliant Blue G250 method might become the method of choice for the determination of total urinary protein.
D001536:Bence Jones Protein / Q000652:urine; D001737:Biuret; D003920:Diabetes Mellitus / Q000652:urine; D005188:False Negative Reactions; D006801:Humans; D007676:Kidney Failure, Chronic / Q000652:urine; D008722:Methods; D011507:Proteinuria / Q000652:urine*; D012394:Rosaniline Dyes; D013223:Statistics as Topic
D012394:Rosaniline Dyes; D001737:Biuret; D001536:Bence Jones Protein; C004692:coomassie Brilliant Blue
Quantitative Proteinbestimmung im Harn mit dem Proteinbindungsfarbstoff Coomassie Brilliant Blau G250.