Isoelectric focusing of globin chains for antenatal diagnosis of beta 0-thalassemia.
A recently developed isoelectric focusing technique for human globin chain separation has been applied to the antenatal diagnosis of beta 0-thalassemia in Sardinia. Results obtained with this method show a complete concordance with those obtained by the currently-in-use chromatographic separation of globin chains by carboxymethyl-cellulose. The ease with which several samples (up to 20) can be simultaneously processed and analyzed by a single operator and the very simple equipment required make this new method ideal for the antenatal diagnosis of beta 0-thalassemia and could encourage a more widespread use of prenatal diagnosis of thalassemias.
D000755:Anemia, Sickle Cell / Q000175:diagnosis; D001345:Autoradiography; D002266:Carboxymethylcellulose Sodium; D002845:Chromatography; D005260:Female; D005914:Globins / Q000032:analysis* / Q000096:biosynthesis; D006801:Humans; D007525:Isoelectric Focusing; D011247:Pregnancy; D013789:Thalassemia / Q000175:diagnosis*
D005914:Globins; D002266:Carboxymethylcellulose Sodium