title: Human monoclonal antibodies to lung-cancer antigens.
abstract: Lymphocytes obtained from hilar and bronchial lymph nodes from 23 patients undergoing radical surgery for carcinoma of the bronchus were fused with established rat or mouse myeloma lines. 62% of the resultant hybrids were found to be secreting human Ig detected by a sensitive staphylococcal Protein A-coupled SRBC assay. Immunoglobulins synthesized by such hybrids were internally labelled with 3H-lysine and their antibody activity against a variety of membrane preparations determined. Nine monoclonal antibodies were found which bound to molecules on lung-cancer membranes and not on normal lung membranes from the same patient.
mesh_terms: D000368:Aged; D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D005260:Female; D006801:Humans; D006822:Hybrid Cells; D007136:Immunoglobulins / Q000096:biosynthesis; D008175:Lung Neoplasms / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008875:Middle Aged; D051381:Rats
chemical_list: D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D007136:Immunoglobulins