[Studies on establishment and maintenance of persistent infection with measles virus in NC-37 cell. 1. Production of DNA-synthesis-suppressing factor (DSF) in NC-37 cell infected with measles virus].
Wild strains of measles virus, such as Edmonston, Toyoshima and freshly isolated KM strain which had not a capacity to establish a persistent infection in NC-37 cell inhibited 80-90 percent of host cellular DNA synthesis, while attenuated or subacute sclerosing panencephalitis (SSPE) viruses, such as Schwarz, Halle and Mantooth which had a capacity to establish persistent infection inhibited about 50 percent of host cellular DNA synthesis 6 or 7 days after infection of virus. However, inhibition of DNA synthesis in cells infected with attenuated or SSPE virus recovered gradually to the same level of control cell within two weeks after infection of virus, and persistently infected cells were not suppressed cellular DNA synthesis in synchronized cell culture. NC-37 cells infected with wild strain produced host cellular DNA-synthesis-suppressing factor (DSF) into culture fluid, but it could detect no such factor in culture fluid of cells infected with attenuated, SSPE virus, or carried virus and persistently infected cell. Furthermore, persistently infected cell treated wtih DSF caused the suppression of DNA synthesis, and so these cells died of metabolic inhibition within one week after inoculation of DSF. NC-37 cell infected with Mantooth strain was inhibited the establishment of persistent infection by the treatment of cell with DSF.
D002478:Cells, Cultured; D004247:DNA / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000502:physiology; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D008459:Measles virus / Q000502:physiology*
D004247:DNA