Diameter of the cell-to-cell junctional membrane channels as probed with neutral molecules.
The cell-to-cell channels in the junctions of an insect salivary gland and of insect and mammalian cells in culture were probed with fluorescent molecules-neutral linear oligosaccharides, neutral branched glycopeptides, and charged linear peptides. From the molecular dimensions of the largest permeants and smallest impermeants the permeation-limiting channel diameter was obtained: 16 to 20 angstroms for the mammalian cells and 20 to 30 angstroms for the insect cells.
D000818:Animals; D002460:Cell Line; D002683:Chironomidae; D005456:Fluorescent Dyes; D006020:Glycopeptides / Q000378:metabolism*; D007365:Intercellular Junctions / Q000648:ultrastructure*; D051379:Mice; D008807:Mice, Inbred BALB C; D008958:Models, Molecular; D009844:Oligosaccharides / Q000378:metabolism*; D011487:Protein Conformation; D012469:Salivary Glands / Q000648:ultrastructure*; D013045:Species Specificity
D005456:Fluorescent Dyes; D006020:Glycopeptides; D009844:Oligosaccharides