How pleural mesothelial cells react in vitro with chrysotile fibres.
A culture system of rat pleural mesothelial cells has been developed for short-term experiments and long-term maintenance. In short-term experiments, endocytosis of chrysotile fibres (100 microgram/ml) has been demonstrated by electron microscopy of the pleura in situ. Structural modifications, mainly cytoplasmic vacuolization, were observed when the cells were cultured with sublethal doses of chrysotile (0.5, 1, 2 and 10 micrograms/ml). Untreated cells had a mean population doubling time of 37 hours. When the cells were treated with 2 or 10 micrograms/ml, the mean population doubling time was slightly increased.
D000818:Animals; D001194:Asbestos / Q000494:pharmacology* / Q000592:standards; D002478:Cells, Cultured; D004705:Endocytosis; D004727:Endothelium / Q000473:pathology / Q000648:ultrastructure; D008854:Microscopy, Electron; D010994:Pleura / Q000473:pathology* / Q000648:ultrastructure; D051381:Rats; D013997:Time Factors; D014357:Trypsin
D001194:Asbestos; D014357:Trypsin