The use of quantitative cytochemistry to monitor human tumour cells in culture.
The prerequisites of any method used to identify human tumour cells in short term culture are specificity, stability, sensitivity and simplicity. This paper looks critically at the use of nuclear DNA measurement combined with 3H thymidine autoradiography as the method of choice in the light of these four requirements. We conclude that although the combined method has some disadvantages, it has many advantages and is particularly appropriate when only a limited number of cells is available from a patient biopsy. Our results show clearly that monitoring procedures are required to verify that the cultured cells are abnormal, particularly after passage.
D000008:Abdominal Neoplasms / Q000032:analysis; D000230:Adenocarcinoma / Q000032:analysis; D000818:Animals; D001345:Autoradiography; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002277:Carcinoma / Q000032:analysis; D002460:Cell Line; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000032:analysis; D006801:Humans; D051379:Mice; D009369:Neoplasms / Q000032:analysis*
D002272:Carcinoembryonic Antigen; D004273:DNA, Neoplasm