[Influence of oocytes upon proliferation of cultured granulosa cells (author's transl)].
Granulosa cells (G-C)  collected from mature follicles of human and bovine ovaries were cultured with an oocyte from mature follicles of human, bovine and porcine ovaries to investigate the influence of the oocyte upon the proliferation of G-C as well as the differentiation of G-C. For this purpose, G-C were compared in mitotic index (MI) between those cultured with oocyte and without oocyte control at the fourth day of culture. 1) G-C cultured with an autogenous oocyte--A bovine oocyte cultured with bovine G-C increased in MI 2.8 times compared with the control. Human G-C cultured with a human oocyte also appeared to raise the MI 2.7 times compared with the control. 2) G-C culture with heterogeneous oocyte--Moreover, a porcine oocyte cultured with bovine G-C acted to increase the MI to 2.7 times that of the control. An addition of a porcine oocyte to human cultured G-C also almost doubled the MI as compared with that of the control. 3) Within 2 days after culture of G-C with an auto- or hetero-genetic oocyte from which the cumulus oophorous had been removed, the oocyte was surrounded by a dense mass of small type of G-C, which resembled follicular G-C morphologically and indicated a high value of MI. The cells on the periphery from the oocyte, however, were clearly enlarged differing from those close to the oocyte and began to resemble lutein cells as the culture days went on. 4) Instead of the oocyte, sterilized muscle, cartilage and bone tissues were cultured with the G-C as controls. However, no binding of G-C with these tissues were found as was verified between G-C and the oocyte. These results suggest that the oocyte may characteristically bind with G-C to accelerate the multiplication and inhibit the luteinization of G-C.
D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D006107:Granulosa Cells / Q000254:growth & development*; D006801:Humans; D008940:Mitotic Index; D009865:Oocytes / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D013045:Species Specificity; D013552:Swine