[DNA methylation in wheat seedling and influence of phytohormones].
A labelled DNA is synthesized in the etiolated wheat seedlings 72 hrs after a single addition of [2-14C]orotic acid (1 mCi per 250 seedlings). This DNA does not differ in the methylation degree of cytosine residues and base composition (radioactivity ratios 100.m5C/(C+m5C) and T/(C++m5C) from total seeding DNA. Thus, the radioactivity ratio 100.m5C/(C+m5C) represents in fact the methylation degree of cytosine residues in labelled DNA, and [2-14C]orotic acid can be used for the study of DNA methylation in higher plants. The newly synthesized DNA (21 hr incubation of seedlings with radioactive precursor) is methylated about 2 times less in comparison with fully synthesized radioactive DNA and total seedling DNA. This supports the assumption on a step-wise character of replicative DNA methylation in plants. Phenylacetic acid (30 mg/l) and 6-benzylaminopurine (l mg/l) do not influence the post-replicative DNA methylation, but auxin (FAA) very strongly inhibits the replicative DNA methylation. The phytohormone-induced changes in replicative DNA methylation are considered as a possible mechanism for regulation of DNA replication and, probably, of cell differentiation in higher plants.
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D001593:Benzyl Compounds; D002250:Carbon Radioisotopes; D003596:Cytosine / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D007210:Indoleacetic Acids / Q000494:pharmacology; D007701:Kinetin; D008745:Methylation; D009963:Orotic Acid / Q000378:metabolism; D010648:Phenylacetates / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010944:Plants / Q000378:metabolism*; D011687:Purines; D014908:Triticum / Q000378:metabolism
D001593:Benzyl Compounds; D002250:Carbon Radioisotopes; D007210:Indoleacetic Acids; D010648:Phenylacetates; D010937:Plant Growth Regulators; D011687:Purines; D009963:Orotic Acid; D003596:Cytosine; D004247:DNA; D000225:Adenine; C480551:benzylaminopurine; D007701:Kinetin
Metilirovanie DNK v prorostkakh pshenitsy i vliianie fitogormonov.