[Kinetic characteristics and effect of sodium fluoride on AMP-aminohydrolase from rat liver hyaloplasm].
NaF in various concentrations is shown to have different effects on the rat liver AMP-aminohydrolase. At constant initial level of AMP in samples the NaF 5-25 mM concentrations favour the enzyme activation and the NaF 5-150 mM concentrations inhibit the AMP deamination. The character of sodium fluoride effect is also determined by the substrate concentration in samples. With the presence of 2-10 mM of AMP and 15 mM sodium fluoride in the reaction mixture the AMP-aminohydrolase activity rises. With the rise of the substrate concentration in the sample the positive kinetic cooperation is replaced by the negative one. The maximal reaction rate is reduced. The Hill coefficient in the control and in the sample with 15 mM of NaF is identical and equals 2.5.
D000659:AMP Deaminase / Q000378:metabolism*; D000818:Animals; D005459:Fluorides / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D009709:Nucleotide Deaminases / Q000378:metabolism*; D051381:Rats; D012969:Sodium Fluoride / Q000494:pharmacology*
D012969:Sodium Fluoride; D009709:Nucleotide Deaminases; D000659:AMP Deaminase; D005459:Fluorides
Kineticheskaia kharakteristika i vliianie ftorida natriia na AMP-aminogidrolazy iz gialoplazmy pecheni krys.