Demonstration of a hematopoietic stem cell antigen (SC-1) on a murine lymphoma and isolation of variants lacking the antigen.
Murine multipotential hematopoietic stem cells (CFU-s) bear an antigen (SC-1) which is recognized by heterologous antisera to mouse brain. We have found that cloned Thy-1 negative variants of the T-cell lymphoma RL male 1 are sensitive to complement-mediated cytolysis by anti-brain serum and can absorb the anti-stem cell activity from the antiserum. We have isolated several subclones derived from a primary Thy-1 negative variant which are not susceptible to anti-brain serum. The surface of the resistant lines has little or no antigen capable of binding anti-mouse brain antibodies as measured by either immunofluorescence or a radioimmunoassay. These lines are also unable to absorb the antibodies responsible for the cytotoxic effect of rabbit anti-mouse brain serum against CFU-s. We conclude that the predominant antigen, serologically detectable on Thy-1 negative variants of RL male 1, is SC-1.
D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics*; D002999:Clone Cells / Q000276:immunology; D014644:Genetic Variation; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D011817:Rabbits
D000951:Antigens, Neoplasm