[Conformation of cytochrome b5 in soluble form and incorporated into artificial membranes].
It was shown that 35% of the peptide groups, which form the right alpha-helices, are presented in the soluble fraction of cytochrome b5. The incorporation of cytochrome b5 into egg lecithine liposomes increases the number of alpha-helices up to 51%. On the contrary, the cytochrome incorporation into microsomal lipid liposomes slightly increases the degree of spiralization. The reduction of cytochrome b5 haem slightly decreases alpha-helices in the soluble haemoprotein as well as in the one incorporated into the artificial membrane irrespective of its phospholipid content.
D003580:Cytochromes; D015786:Cytochromes b5; D004355:Drug Stability; D006418:Heme; D007700:Kinetics; D008081:Liposomes; D008861:Microsomes / Q000032:analysis; D010084:Oxidation-Reduction; D010713:Phosphatidylcholines; D011487:Protein Conformation; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet
D003580:Cytochromes; D008081:Liposomes; D010713:Phosphatidylcholines; D006418:Heme; D015786:Cytochromes b5
Konformatsionnoe sostoianie rastvorimogo i vstroennogov iskusstvennuiu membranu tsitokhroma b5.