[Effects of certain biologically active compounds and bivalent cations on lactate and malate dehydrogenase activities in cerebral cellular fractions].
It is shown that acetylcholine, noradrenaline and serotonin have no effect on the lactate (LDG) and malate dehydrogenase (MDG) activities in mitochondria, mitoblasts and supernatant fluid of the rat brain tissue. A combined effect of noradrenaline and serotonin with acetylcholine increases the activity of MDG in the mitochondria but does not change that of LDG. In the mitoblast MDG was less active. After treatment with some detergents noradrenaline reduces that LDG activity in the mitochondria and supernatant fluid and did not change the MDG one. Serotonin enhances the LDG and decreased the MDG activity. In the presence of Mg2+ an increase in the LDG activity in supernatant fluid is accompanied by a decrease in the mitochondria while the MDG activity is unchanged. Ca2+ activates MDG in the mitochondria and did not affect the LDG activity.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002413:Cations, Divalent; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D009638:Norepinephrine / Q000494:pharmacology*; D051381:Rats; D012701:Serotonin / Q000494:pharmacology*; D013347:Subcellular Fractions / Q000201:enzymology
D002413:Cations, Divalent; D012701:Serotonin; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D008274:Magnesium; D000109:Acetylcholine; D009638:Norepinephrine
Vliianie nekotorykh biologicheski aktivnykh soedineniĭ i dvukhvalentnykh kationov na laktat- i malatdegidrogenaznuiu aktivonost' v kletochnykh fraktsiiakh golovnogo mozga.