Effect of elevated blood glucose levels on hepatic microsomal enzyme system in rats.
Elevated blood glucose levels attained by ad libitum drinking of 20% glucose solution inhibited in vivo O-dealkylation associated with the hepatic microsomal enzyme system. Significant inhibition of biotransformation was demonstrated after 1 day, with the maximum occurring at 3 days. Inhibition was followed by a tendency to return to normal activity. Serum levels of substrate and product were determined by liquid chromatographic techniques using 55% acetonitrile and 45% 0.1 M acetic acid as the mobile phase.
D000818:Animals; D001711:Biotransformation; D001786:Blood Glucose / Q000378:metabolism*; D002853:Chromatography, Liquid; D003640:Dealkylation; D007213:Indomethacin / Q000097:blood; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D013997:Time Factors
D001786:Blood Glucose; D007213:Indomethacin