title: Distribution of cholinesterases of rabbit skeletal muscle.
abstract: Two cholinesterase (ChE) fractions were extracted from homogenates of rabbit skeletal muscle. One of them the sarcoplasmic ChE could be dissolved quantitatively with solution A. Following the extraction with solution B the myofibrillar ChE fraction could be recovered. The ChE-activity of the sarcoplasmic extract was about 38% and the activity of myofibrillar extract was about 50% of the ChE-activity of muscle homogenates. According to our calculations about 10-12% of the total ChE-activity is insoluble. By the aid of PAGE seven zones of ChE-activity could be distinguished in the sarcoplasmic extract. The activity of myofibrillar ChE-fraction was obtained by chromatographic purification of LMM. The LMM1 proved to be inhomogeneous with PAGE, however, only one fraction displayed ChE-activity. Immune-serum produced against myosin did not give reaction with purified sarcoplasmic ChE, only with the myofibrillar fraction. Our results suggest that the heterogeneous distribution of muscle ChE-es in different molecular forms is not an artifact.
mesh_terms: D000818:Animals; D002802:Cholinesterases / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits
chemical_list: D009124:Muscle Proteins; D002802:Cholinesterases