title: Micrococcal nuclease cleavage of chromatin displays nonrandom properties.
abstract: A statistical analysis of the products of digestion of chicken erythrocyte chromatin by micrococcal nuclease was used to test for randomness of the cutting process. DNA fragment size classes corresponding to mononucleosome, dinucleosome, trinucleosome, tetranucleosome, and all fragments larger than tetranucleosome were evaluated. In every case, fragments in the mononucleosome and greater-than-tetranucleosome classes were produced in excess of the level expected on the basis of random cleavage while those in the dinucleosome-tetranucleosome classes exhibited a shortage. The pattern of nonrandomness appears to depend on substrate size: the magnitude of deviations from randomness was large when substrates of genomic size are compared with polynucleosomal segments whereas the direction of deviation is identical. Nonrandomness was independent of ionic conditions known to affect the state of chromatin condensation and also appeared to be unaffected by depletion of histones H1 and H5. The possible universality of nonrandom cleavage was suggested when other data from the literature was analyzed. Some possible mechanisms to account for this property are discussed.
mesh_terms: D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000032:analysis*; D004247:DNA / Q000097:blood*; D004912:Erythrocytes / Q000032:analysis*; D046911:Macromolecular Substances; D008836:Micrococcal Nuclease; D009707:Nucleosomes / Q000032:analysis
chemical_list: D002843:Chromatin; D046911:Macromolecular Substances; D009707:Nucleosomes; D004247:DNA; D008836:Micrococcal Nuclease