Template-directed synthesis of oligoadenylate. Template effect of oligouridylates and catalytic activity of Pb2+ ion.
Template-directed condensation of adenosine-5'-phosphorimidazolide was conducted in the presence of oligouridylate template. Oligouridylates with more than eight chain units can serve as a template and facilitate oligoadenylate formation. Internucleotide linkage of the oligoadenylate obtained by this reaction was mainly 2'-5'. Addition of Pb2+ ion catalyst to this reaction system promoted the formation of long oligoadenylates. The template with Pb2+ ion catalyst preferentially gave 3'-5' linked oligoadenylates.
D000227:Adenine Nucleotides / Q000138:chemical synthesis*; D007202:Indicators and Reagents; D007854:Lead; D008722:Methods; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D013698:Templates, Genetic; D014500:Uracil Nucleotides
D000227:Adenine Nucleotides; D007202:Indicators and Reagents; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D014500:Uracil Nucleotides; C027916:oligo(U); D007854:Lead; C023505:2',5'-oligoadenylate