title: Initial biotransformations of daunorubicin to aglycones by rat liver microsomes.
abstract: Anaerobic incubations of rat liver microsomes convert daunorubicin into at least six or seven aglycones in the presence of reduced nicotinamide adenine dinucleotide phosphate or a reduced nicotinamide adenine dinucleotide phosphate-generating system. The partial identification of some of the aglycones is based on cochromatographs of chemically synthesized derivatives by isocratic high-pressure liquid chromatography. The first major reaction is the reductive cleavage of daunorubicin to 7-deoxydaunorubicin aglycone which is converted to another product that cochromatographs with 7-deoxydaunorubicinol aglycone. The reaction follows first-order kinetics in a linear sequential pathway. Formation of 7-hydroxydaunorubicin aglycone is also identified as a product. Other reaction products are not yet identified.
mesh_terms: D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D003630:Daunorubicin / Q000378:metabolism*; D004317:Doxorubicin / Q000378:metabolism; D005453:Fluorescence; D066298:In Vitro Techniques; D007700:Kinetics; D008862:Microsomes, Liver / Q000378:metabolism*; D009279:Naphthacenes / Q000032:analysis / Q000378:metabolism*; D051381:Rats
chemical_list: D009279:Naphthacenes; D004317:Doxorubicin; C019318:daunomycinone; D003630:Daunorubicin