[Gas chromatographic determination of thiocyanate in vegetable and animal foods and in human serum following paper chromatographic separation (author's transl)].
Paper chromatographic separation of thiocyanate is the first step in determining non-bound thiocyanate in homogenized animal and vegetable foods and in human serum. The thiocyanate eluated from the paper chromatogram is brominated and then determined as cyanogen bromide using a gas chromatograph equipped with an electron-capture detector. Interfering substances (mainly ethanol-unprecipitable protein components, amino acids and cyanide from glycosidic precursors) are eliminated by this procedure.
D002854:Chromatography, Paper / Q000379:methods; D005504:Food Analysis; D006801:Humans; D008460:Meat / Q000032:analysis; D013861:Thiocyanates / Q000032:analysis* / Q000097:blood; D014675:Vegetables / Q000032:analysis
D013861:Thiocyanates
Gaschromatografische Bestimmung des Thiocyanats in pflanzlichen und tierischen Lebensmitteln sowie Humanserum nach papierchromatografischer Abtrennung.