title: Palate morphogenesis. VI. Identification of stellate cells in culture.
abstract: Mesenchymal cells from the palate of mouse embryos at day 14.5 of gestation produce a minor population of stellate cells in culture. These cells are often bipolar and spindle-shaped with long cytoplasmic processes similar to neural-crest cells. Culturing of explants of palatal mesenchyme enriched for this type of cell. Stellate cells were the first to migrate from explants, followed by fibroblast-like cells and then by squamous cells. The majority of the cells in the explant were fibroblast-like. Squamous cells were present mostly in the anterior and mid-palate and least frequently in those from the posterior palate. They may represent tooth-germ epithelium. When pieces of palate were dissected out and cultured for enrichment of non-muscle contractile systems, most of the migrating cells were stellate. These may represent the highly migratory cells that are, in part, responsible for elevation of the palate shelf. Serotonin was measured in cultured mesenchymal cells from the palate. Its occurrence is consistent with regulation of movement of palate cells.
mesh_terms: D000818:Animals; D002465:Cell Movement; D002478:Cells, Cultured; D051379:Mice; D009024:Morphogenesis; D010159:Palate / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism
chemical_list: D012701:Serotonin