The presence of blood group and lymphocyte antigens on porcine granulocytes.
Suspensions of highly viable (less than 95%) granulocytes minimally contaminated by other cell types were isolated from the peripheral blood of pigs by a single centrifugation with low molecular weight dextran and after preferential lysis of erythrocytes by hypotonic shock. A complement-dependent cytotoxic test showed the presence of antigens of the SLA major histocompatibility complex, the SLB leucocyte system and the A and E blood group systems on the granulocytes. Some SLA typing reagents against class I (SD) antigens did not react with granulocytes, however, or yielded dubious reactions. The findings showed that the reactivity of SLA sera resembles the reactivity fo human HLA sera. The results also show that compatibility in the SLA, SLB, A and E systems will have to be taken into account when preparing alloimmune sera for the determination of granulocyte-specific antigens of pigs.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001789:Blood Group Antigens / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006098:Granulocytes / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008285:Major Histocompatibility Complex; D009504:Neutrophils / Q000276:immunology; D013552:Swine
D000954:Antigens, Surface; D001789:Blood Group Antigens