title: Total lipid and membrane lipid analysis of normal animal and human lenses.
abstract: Comparisons of lens fiber cell membrane isolation methods were made. Although membrane lipid yield from stirring overnight in hypotonic medium followed by low-speed centrifugation was comparable to that from homogenization and high-speed centrifugation, losses in phosphatidyl ethanolamine were observed with the former method. Lyophilization with vacuum replacement by nitrogen did not exert any deleterious effects. A significant difference in total lens lipid was observed between human and the three species of animals investigated.
mesh_terms: D000368:Aged; D000818:Animals; D002417:Cattle; D005612:Freeze Drying; D006801:Humans; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000032:analysis*; D008055:Lipids / Q000032:analysis*; D008875:Middle Aged; D011817:Rabbits
chemical_list: D008055:Lipids