Interrelationships of the proteins of two parvoviruses (densonucleosis virus types 1 and 2).
Densonucleosis viruses (types 1 and 2) contain four major structural polypeptides with a total molecular weight in excess of the coding capacity of the DNA. Peptide maps obtained by limited proteolysis of isolated (125)I-labeled proteins of both virus types indicate a common origin of the virus proteins and homology between the different viruses. The structure of densonucleosis virus type 2 and its homologous top component (naturally occurring empty particles) was compared by proteolysis using several proteases and the bifunctional cross-linking reagents dimethylsuberimidate (DMS) and dimethylmalonimidate. Similar susceptibilities of both components with proteases were obtained. The top components alone were accessible to the action of the cross-linking reagent DMS. The lowest molecular weight major structural polypeptide was most resistant to the action of the proteases and DMS.
D004591:Electrophoresis, Polyacrylamide Gel; D007304:Insect Viruses / Q000032:analysis* / Q000648:ultrastructure; D010321:Parvoviridae / Q000032:analysis* / Q000648:ultrastructure; D010446:Peptide Fragments / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis*
D010446:Peptide Fragments; D014764:Viral Proteins