Action spectra for ultraviolet light-induced transformation of human cells to anchorage-independent growth.
We have determined action spectra for transformation of human embryonic skin and muscle fibroblasts to anchorage-independent growth. Tests under our experimental conditions indicate that reciprocity holds for photon rate and exposure time and that all the dose-effect curves in the wavelength range of 248 to 297 nm are smaller. These data can be used to construct action spectra with a maximum at about 265 nm, which do not implicate moieties other than nucleic acids as absorbers in the transformation process.
D002471:Cell Transformation, Neoplastic / Q000528:radiation effects; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D009132:Muscles; D012867:Skin; D013056:Spectrophotometry, Ultraviolet; D014466:Ultraviolet Rays
D004247:DNA