A new method for detection of drug-binding proteins using a parallel-flow dialysis technique.
A parallel-flow dialysis technique utilizing a Technicon dialyzer and a constant-flow system has been described for the detection of drug-binding proteins. The effect of temperature, flow-rate and drug concentration was investigated by measuring the efficiency of dialysis and detecting the binding of methyl orange to bovine serum albumin. The larger response was shown to be achieved by increasing the efficiency of dialysis or the drug concentration. The present method will enable the continuous monitoring of drug-binding proteins.
D000818:Animals; D002352:Carrier Proteins / Q000097:blood* / Q000378:metabolism; D002417:Cattle; D003956:Dialysis / Q000379:methods; D006801:Humans; D004364:Pharmaceutical Preparations / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism
D002352:Carrier Proteins; D004364:Pharmaceutical Preparations; D012710:Serum Albumin, Bovine