[Permeability of acetone-glued diffusion chambers with walls made of the HAWP millipore filter for erythrocytes in in vivo cultivation].
When studying the differentiation potencies of cells of the chick embryo at the stage of early blastoderm in the diffusion chambers with walls of millipore filter HAWP placed intraperitoneally, it is necessary to be sure that the chambers are impermeable for the blood cells. The chambers made with the use of aceton for glueing the millipore filter onto the ring of organic glass may be permeable for erythrocytes incapable of active movement. The appearance of erythrocytes in the chamber creates the illusion of hemopoiesis in the culture. Within several days of cultivation in the abdominal cavity, a monolayer of macrophages is formed outside the chamber which prevents the penetration of erythrocytes in the chamber.
D000096:Acetone / Q000494:pharmacology*; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D004058:Diffusion; D004912:Erythrocytes / Q000502:physiology*; D008722:Methods; D008851:Micropore Filters; D010539:Permeability; D051381:Rats; D011919:Rats, Inbred Strains
D000096:Acetone
Pronitsaemost' skleennykh atsetonom diffuzionnykh kamer so stenkami iz milliporovogo fil'tra HAWP (millipore) dlia éritrotsitov v usloviiakh kul'tivirovaniia in vivo.