Membrane protein detachment and mitogenic stimulation induced by alkaline pH.
The stimulatory effects on DNA-synthesis by a short alkaline treatment in low serum concentration on quiescent 3T3-cells in sparse cultures was investigated. It was found that a short alkaline treatment performed at 4 degrees C stimulated quiescent cell to initiate DNA-synthesis to the same extent as a short alkaline treatment at 37 degree C. Furthermore, an alkaline treatment caused a detachment of membrane bound proteins. The quantity of these proteins that were detached during the alkaline treatment, was closely correlated to the stimulatory effects on DNA-synthesis.
D000818:Animals; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008934:Mitogens / Q000494:pharmacology*; D011247:Pregnancy; D013696:Temperature
D008565:Membrane Proteins; D008934:Mitogens; D004247:DNA