title: Growth in culture of adenocarcinoma cells from the small intestine of sheep.
abstract: Explant cultures were initiated from adenocarcinoma of the small intestine in sheep and from various metastases. Several cell types grew, most being fibroblastic in nature. However, 2 cultures yielded mixed cells which arranged themselves into areas of epithelial-like cells surrounded by fibroblast-like cells and this pattern was consistent over 30 subcultures and several months of culturing. The epithelial-like cells were separated from the others by the use of a modified medium containing citrulline or by sedimentation through a bovine serum albumin solution. Various properties, including their growth rate in 5% and 0.5% serum, the absence of surface fibronectin and their ability to grow in semi-solid agar, indicated that they may represent carcinoma cells. Screening for virus production from these cells and all other explant cultures proved negative.
mesh_terms: D000230:Adenocarcinoma / Q000382:microbiology / Q000648:ultrastructure / Q000662:veterinary*; D000818:Animals; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D007414:Intestinal Neoplasms / Q000382:microbiology / Q000648:ultrastructure / Q000662:veterinary*; D007421:Intestine, Small / Q000648:ultrastructure; D007621:Karyotyping; D008207:Lymphatic Metastasis; D009858:Oncogenic Viruses / Q000302:isolation & purification; D012756:Sheep