Methylation of hamster DNA by the carcinogen N-nitroso-bis (2-oxopropyl)amine.
The alkylation of hamster liver, lung and pancreas DNA by [1-14C]- and [2,3-14C]N-nitrosobis (2-oxopropyl) amine (BOP) has been examined. The specific activity of pancreas DNA after [2,3-14C]BOP administration was only 2% of that when [1-14C]BOP was given. 7-Methylguanine, but not O-6-methylguanine, was found in hydrolysates of liver and pancreas DNA. Nearly equal amount of alkylation were produced in the liver when [1-14C]- and [2,3-14C]BOP were given. At least one-half of the radioactivity in the liver was associated with N-alkylated purines, whereas only 20% was in this form in the pancreas.
D000818:Animals; D002273:Carcinogens / Q000378:metabolism* / Q000633:toxicity; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D008647:Mesocricetus; D008745:Methylation; D009602:Nitrosamines / Q000378:metabolism*; D010179:Pancreas / Q000378:metabolism
D002273:Carcinogens; D009602:Nitrosamines; C013622:nitrosobis(2-oxopropyl)amine; D004247:DNA