Some aspects of chemical synthesis of oligoribonucleotides.
A simple synthesis of ribothymidine 3'-phosphate from uridine 3'-phosphate was described. Crystalline 5'-O-trityl- or 5'-O-monomethoxytrityl-derivatives of ribonucleotides are easily prepared and purified in relatively large batches through choices of appropriate solvents. Acylimidazoles together with a strong nitrogeneous base have been successfully used for the acylation of the hydroxyl and amino groups of the nucleoside and nucleotide, and the reaction mechanism was studied. The relative stability of different blocking groups of guanosine 3'-phosphate was compared. Oligoribonucleotide fragments including an octaribonucleotide of the T psi C- and D-loop of yeast alanine tRNA were synthesized. Quantitative analyses of the common ribo-nucleosides and -nucleotides were done by a modified RPC method.
D001483:Base Sequence; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008722:Methods; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D014500:Uracil Nucleotides; D014542:Uridine Monophosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis
C031035:3'-ribothymidylic acid; D007202:Indicators and Reagents; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D014500:Uracil Nucleotides; C031034:3'-uridylic acid; D014542:Uridine Monophosphate