title: Cell-surface glycoprotein synthesis during differentiation of chicken erythroblasts transformed by temperature-sensitive avian erythroblastosis virus.
abstract: Chicken erythroblasts transformed by a temperature-sensitive mutant of avian erythroblastosis virus (ts34 AEV) have a greatly increased haemoglobin content (Graf, T., N. Ade and H. Beug: Nature 275, 496-501 (1978)) if allowed to grow for 3-5 days at the non-permissive temperature (41 degrees C), instead of the permissive temperature (35 degrees C) of the virus. Cell-surface molecular changes during this differentiation were investigated by examining the glycoproteins synthesized by a ts34-transformed erythroblast cell line. These cells synthesized a greatly increased amount of a 94,000 molecular weight erythrocyte cell-surface glycoprotein beginning 2-6 h after a shift in growth temperature from 35 degrees to 41 degrees C, consistent with the proposal that such a shift releases these transformed cells from a differentiation block.
mesh_terms: D001355:Alpharetrovirus; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D002645:Chickens / Q000097:blood; D004900:Erythroblasts / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D004920:Erythropoiesis; D005914:Globins / Q000096:biosynthesis; D006023:Glycoproteins / Q000097:blood*; D008565:Membrane Proteins / Q000097:blood*; D013696:Temperature
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins; D005914:Globins