DNA analysis and sorting of viable mouse testis cells.
The dye Hoechst 33342 and a 2-parameter cell sorter have been used to measure DNA content in viable testis cells and to sort pachytene spermatocytes and round spermatids from adult mouse testis to virtually 100% homogeneity. Early diploid spermatogenic cells were enriched to 90%, a 10-fold purification. The capability for viable sorting of most testis cell types to homogeneity in numbers suitable for many biochemical applications is demonstrated.
D000818:Animals; D001562:Benzimidazoles; D002453:Cell Cycle; D002469:Cell Separation / Q000379:methods*; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes; D007985:Leydig Cells / Q000032:analysis; D008297:Male; D051379:Mice; D013087:Spermatids / Q000032:analysis; D013090:Spermatocytes / Q000032:analysis; D013737:Testis / Q000166:cytology*
D001562:Benzimidazoles; D005456:Fluorescent Dyes; D004247:DNA; C017807:bisbenzimide ethoxide trihydrochloride