Nonenzymic hydrogen transfer between reduced and oxidized pyridine nucleotides.
Mixtures of NADH and NADP+ or NADPH and NAD+ were incubated and periodically assayed for hydrogen transfer by measuring the formation of NADPH and NADH with glutathione reductase (NAD(P)H: oxidized-glutathione oxidoreductase, EC 1.6.4.2) and lactate dehydrogenase (L-lactate: NAD+ oxidoreductase, EC 1.1.1.27), respectively. Each mixture showed a steady nonenzymic transfer of hydrogen from the reduced to the oxidized pyridine nucleotide to yield a product that was completely enzymically active. The results demonstrate the specific nonenzymic transfer of hydrogen from NADH and NADPH to the pyridine C-4 position of NADP+ and NAD+, respectively.
D005980:Glutathione Reductase / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction
D009243:NAD; D009249:NADP; D006859:Hydrogen; D007770:L-Lactate Dehydrogenase; D005980:Glutathione Reductase