title: Establishment and biological properties of a guinea pig colonic adenocarcinoma cell line induced by N-methyl-N-nitrosourea.
abstract: A colonic adenocarcinoma cell line, designated GPC-16, was established in culture from tissue removed from an outbred guinea pig which received 56 mg of N-methyl-N-nitrosourea intrarectally over a 28-week period. the GPC-16 has been maintained for more than 30 in vitro passages and was tumorigenic in both the autochthonous host and nude mice. The cells were aneuploid with a wide range in chromosome number (54 to 172) in a bimodal distribution. The cells grew in vitro as a tight monolayer and demonstrated numerous desmosomes by electron microscopy. In vitro-passaged cells had a doubling time of 36 hr and a 5.1% plating efficiency in agar.
mesh_terms: D000230:Adenocarcinoma / Q000139:chemically induced* / Q000473:pathology / Q000648:ultrastructure; D000782:Aneuploidy; D000818:Animals; D002452:Cell Count; D002460:Cell Line; D002875:Chromosomes; D003110:Colonic Neoplasms / Q000139:chemically induced* / Q000473:pathology / Q000648:ultrastructure; D003896:Desmosomes / Q000648:ultrastructure; D005260:Female; D006168:Guinea Pigs; D008770:Methylnitrosourea; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology; D009607:Nitrosourea Compounds; D013997:Time Factors
chemical_list: D009607:Nitrosourea Compounds; D008770:Methylnitrosourea