[Effect of cryoprotectors on stability of lysosomes under cooling].
The efficiency of cryoprotectors-polyethylene oxide 400, glycerol, oxyethylated glycerol, dimethyl sulphoxide was studied relative to stabilization isolated lysosomes under different regimes of freezing-thawing. It is established that a decrease in the amount of lysosome hydrolases (RNase, DNase, catepsin D, phosphatase) released to the environment is the most significant in the presence of the mentioned substances uncer slow freezing-thawing.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D006867:Hydrolases / Q000378:metabolism; D008099:Liver / Q000648:ultrastructure; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D011092:Polyethylene Glycols; D051381:Rats
D003451:Cryoprotective Agents; D011092:Polyethylene Glycols; D006867:Hydrolases; D005990:Glycerol; D004121:Dimethyl Sulfoxide
Vliianie krioprotektorov na stabil 'nost' izosom pri okhlazhdenii