Superoxide dismutase activity in thermally stressed Staphylococcus aureus.
The effects of heat and NaCl on the activity of superoxide dismutase from Staphylococcus aureus were examined. A linear decrease in superoxide dismutase activity occurred when S. aureus MF-31 cells were thermally stressed for 90 min at 52% C in 100 mM potassium phosphate buffer (pH 7.2). After 20 min of heating, only 5% of the superoxide dismutase activity was lost. Heating for 60, 90 and 120 min resulted in decreases of approximately 10, 22, and 68%, respectively. The rates of thermal inactivation of superoxide dismutase from S. aureus strains 196E and 210 were similar and slightly greater than those of strains MF-31, S-6, and 181. The addition of NaCl before or after heating resulted in increased losses of superoxide dismutase activity.
D006358:Hot Temperature; D012965:Sodium Chloride / Q000494:pharmacology; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000145:classification / Q000201:enzymology*; D013482:Superoxide Dismutase / Q000378:metabolism*
D012965:Sodium Chloride; D013482:Superoxide Dismutase