The effects of atropine and oxotremorine on acetylcholine release in rat phrenic nerve-diaphragm preparations.
1 Atropine (10(-5) M) enhanced the release of [3H]-acetylcholine from rat isolated hemidiaphragms, previously incubated with [3H-methyl]-choline, stimulated via their phrenic nerves. 2 Oxotremorine (10(-5) M) did not affect the stimulated release of [3H]-acetylcholine but antagonized the facilitatory effects of atropine (10(-5) M). 3 It is suggested that there are presynaptic inhibitory muscarinic receptors that modulate the release of acetylcholine in the phrenic nerves of the rat.
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000494:pharmacology*; D003964:Diaphragm / Q000378:metabolism; D004347:Drug Interactions; D066298:In Vitro Techniques; D009469:Neuromuscular Junction / Q000378:metabolism*; D010095:Oxotremorine / Q000494:pharmacology*; D010791:Phrenic Nerve / Q000378:metabolism; D051381:Rats
D010095:Oxotremorine; D001285:Atropine; D000109:Acetylcholine