Effect of histamine on the mitogenic response of human lymphocytes and its modification by cimetidine and levamisole.
The phytohaemagglutinin (PHA) mitogenic response of human blood lymphocytes demonstrated a dose-dependent inhibition by histamine (in the range 5 X 10(-7) M). Using suboptimal mitogenic doses of PHA the suppression was more pronounced. Stimulation using different doses of pokeweed mitogen (PWM) was not altered by histamine. Purified T cells and a lymphocyte population depleted of histamine-receptor-bearing cells were less sensitive to the histamine effect. In the supernatants of histamine-stimulated cultures, suppressor factor activity was found. Cimetidine could reverse the effect of histamine in a dose-dependent manner. Adding levamisole to the PHA cultures also produced some antihistaminic effect.
D002927:Cimetidine / Q000494:pharmacology*; D006146:Guanidines / Q000494:pharmacology*; D006632:Histamine / Q000494:pharmacology*; D006801:Humans; D007978:Levamisole / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects*; D008934:Mitogens / Q000037:antagonists & inhibitors*; D010835:Phytohemagglutinins / Q000037:antagonists & inhibitors
D006146:Guanidines; D008934:Mitogens; D010835:Phytohemagglutinins; D007978:Levamisole; D002927:Cimetidine; D006632:Histamine