Reduction of exogenous FMN by isolated rat liver mitochondria. Significance to the mobilization of iron from ferritin.
When FMN is added to rat liver mitochondria or mitoplasts it is reduced at a rate of approx. 0.2 nmol . min-1 . mg-1 protein. Sonicated mitochondria do not reduce exogenous FMN. The reduction depends on drainage of reducing equivalents from the respiratory chain at the level of ubiquinone. The net production of reduced FMN is detectable only at oxygen concentrations less than 4-5 muM. The mitochondrial ubiquinol-FMN oxidoreductase provides a mechanism for the coupling of FMN-reduction to the reductive mobilization of iron from ferritin. The results are discussed in relation to the role of ferritin as a donor of iron to the mitochondria.
D000818:Animals; D005293:Ferritins / Q000378:metabolism*; D005486:Flavin Mononucleotide / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D008933:Mitochondrial Swelling; D010084:Oxidation-Reduction; D051381:Rats; D013367:Submitochondrial Particles / Q000378:metabolism*
D005486:Flavin Mononucleotide; D005293:Ferritins; D007501:Iron