An improved method for concurrent isolation of human granulocytes and lymphocytes. Its application in isoelectric focusing electrophoresis.
A modified method of Boyum's technique to isolate granulocytes and lymphocytes from human blood is described. The granulocytes and the lymphocytes separated by this isolation procedure are highly pure, viable, and in sufficient number for subsequent isoelectric focusing studies. Different proteins have been separated by isoelectric focusing. This technique can be useful in the investigation of granulocytic and lymphocytic disorders.
D002469:Cell Separation / Q000379:methods*; D006098:Granulocytes; D006801:Humans; D007525:Isoelectric Focusing; D008214:Lymphocytes