title: Interactions between human lymphoblastoid cells and human fibroblast feeder layers in vitro.
abstract: Lymphoblastoid (LB) cells interact in vitro with human fibroblasts. Cell complexes can be dissociated by trypsin treatment. Binding on fibroblasts is higher for Epstein-Barr virus-producer LB cells than for nonproducers, and depends on the origin of the fibroblastic cells. Stromal fibroblasts isolated from a metastatic lymph node tumor exhibited a highly increased potency of binding LB cells in comparison with fibroblasts isolated from normal breast epidermis or the primary breast tumor from the same patient. After a 10-hour interaction period, LB cells undergo abnormal divisions giving rise to the formation of 'mini cells'.
mesh_terms: D000818:Animals; D001943:Breast Neoplasms; D002448:Cell Adhesion; D002450:Cell Communication; D002455:Cell Division; D002460:Cell Line; D005260:Female; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D007365:Intercellular Junctions / Q000502:physiology; D007399:Interphase; D008207:Lymphatic Metastasis; D008214:Lymphocytes / Q000502:physiology*; D008297:Male; D051379:Mice; D008875:Middle Aged