title: Reduction capacity on nitroblue tetrazolium (NBT) of the normal human spermatozoa.
abstract: Experimental conditions were determined to find out if washed spermatozoids from fertile, health donors are capable of nitroblue tetrazolium (NBT) reduction in a similar way (although in the absence of a substrate) to that of leukocytes. Formazan blue formation obtained suggest the participation of the NADH oxidoreductase mitochondrial enzyme. The sperm capacity for NBT reduction might be take as a reflection of human sperm metabolic capacity. Our results seem to be useful for comparison of reduction (metabolic) capacity of normal sperms with that of individuals with fertility problems.
mesh_terms: D003124:Colorimetry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007248:Infertility, Male / Q000175:diagnosis; D008297:Male; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D010084:Oxidation-Reduction; D013094:Spermatozoa / Q000378:metabolism*; D013696:Temperature; D013778:Tetrazolium Salts / Q000378:metabolism*; D013997:Time Factors
chemical_list: D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium