Correlative study of the ultrastructure and the physiology of the seasonal regression of the epididymal epithelium in the hedgehog Paraechinus micropus.
The seasonal variations in the ultrastructure and physiology of the epididymis of hedgehog were studied in relation to the reproductive functions. Five adult male hedgehogs were sacrificed every alternate month for one calendar year and the epididymis was fixed in Bouin's, Zenker and formol-calcium for light microscopy and in cold buffered glutaraldehyde, post-fixed in osmium tetraoxide for electron microscopy. The epididymal epithelium consists of four types of cells, the principal, the apical, the dark and the basal cells. The principal cells like other steroid synthesizing cells, contain the extensive Golgi apparatus, the smooth and the rough endoplasmic reticulum (SER and RER), the secretory vesicles and the lipid granules during the breeding season, but they are practically devoid of these cell organelles during regression, except for the retarded Golgi and moderate RER. The basal cell, on the other hand, show lipids and well developed organelles during regression but poorly developed structure in the sexually active hedgehog and possibly function as the cells storing lipids during regression and which are subsequently used at the beginning of the recrudescence. The epididymal epithelium recrudesces along with the seminiferous epithelium prior to the spermatozoa reaching the epididymal lumen, whereas the accessory sex glands which are also the extratesticular androgen dependent organs, still show regressed structure. Thus, the ultrastructural and the physiological observations suggest that the principal cells are probably the site of androgen synthesis and they become fully developed along with the cells in the testis on stimulation from the pituitary at the beginning of the recrudescence.
D000818:Animals; D004822:Epididymis / Q000502:physiology* / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure; D006364:Hedgehogs / Q000502:physiology*; D008297:Male; D012621:Seasons; D012726:Sexual Behavior, Animal / Q000502:physiology*