[Evaluation of kinetic parameters of unpurified enzymic systems, method for measuring the concentration of endogenous substrate].
A universal method is proposed for determining concentration of the endogenous substrate S (notwithstanding its concrete nature) in unpurified enzymic systems, whose behaviour is described by the equation of Michaelis - Menten type. The approach is based on measurement and analysis of the values of apparent Michaelis constant, characterizing the formation kinetics of labelled product from the labelled substrate S added to the system. The method for calculating S is at the same time valid for calculating real values of v, V and Km for the samples of unpurified enzymes, which contain unknown amounts of endogenous substrate.
D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D011485:Protein Binding
D004798:Enzymes
Otsenka kineticheskikh parametrov neochishchennykh fermentativnykh sistem, sposob izmereniia kontsentratsii éndogennogo substrata.