Conversion of [4-14C]progesterone to androstenedione in vitro by thecal tissue from the ovary of the domestic fowl (Gallus domesticus).
The conversion of [4-14C]progesterone to androstenedione by thecal tissue homogenates from the large yellow follicles of the hen ovary was measured at two periods of the ovulatory cycle after incubation for 1 h in the presence of cofactors. Production of androstenedione by the largest follicle, F1, was reduced significantly 1-2 h before ovulation compared with 15 h before ovulation, whereas production of an unidentified androgen increased. These differences were not observed in the next largest follicles, F2 and F3. Thecal tissue homogenates from all the follicles converted [4-14C]progesterone to 17 alpha-hydroxyprogesterone, androstenedione and testosterone.
D000728:Androgens / Q000378:metabolism; D000735:Androstenedione / Q000378:metabolism*; D000818:Animals; D002645:Chickens / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D010060:Ovulation; D011374:Progesterone / Q000378:metabolism*; D013799:Theca Cells / Q000378:metabolism*
D000728:Androgens; D000735:Androstenedione; D011374:Progesterone