Solid-phase synthesis of polynucleotides: V. Synthesis of oligodeoxyribonucleotides by the phosphomonotriazolide method.
Synthesis of two oligodeoxyribonucleotides of defined sequences, an undecamer [d(TGCACCATTCT)] and a dodecamer [d(TGGAGCCACTAT)], and tetradecathymidylic acid was described by a simple solid-phase method on a polystyrene resin. The synthesis was performed by the stepwise addition of deoxynucleoside 3'-phosphomonotriazolide to the resin, in the presence of a nucleophilic catalyst, 4-dimethylaminopyridine or N-methylimidazole. Quantitative coupling yield was consistently obtained for each cycle and the desired product was a major peak in the analysis of the final reaction by high performance liquid chromatography on Permaphase AAX.
D001483:Base Sequence; D007202:Indicators and Reagents; D008722:Methods; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009943:Organophosphorus Compounds; D013938:Thymidine Monophosphate; D014230:Triazoles
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D014230:Triazoles; D013938:Thymidine Monophosphate