H2O2 release from filtration leukapheresis-procured leukocytes.
Filtration leukapheresis-procured leukocytes (FL-leukocytes), which were collected by the elution of filtration columns with vigorous tapping, released a certain amount of H2O2 even in the absence of any phagocytic stimuli. Furthermore, FL-leukocytes, eluted with either gentle or vigorous tapping, exhibited a marked release of H2O2 during phagocytosis. The myeloperoxidase (MPO) activity of FL-leukocytes was lower than that of leukocytes collected by the dextran sedimentation method (DS-leukocytes). The data suggest that the release of both H2O2 and MPO from FL-leukocytes may be related to adverse transfusion reactions and abnormal post-transfusion kinetics of FL-leukocytes due to their toxic effects on living cells.
D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007937:Leukapheresis; D007962:Leukocytes / Q000378:metabolism*; D012603:Scopoletin / Q000378:metabolism
D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D012603:Scopoletin