title: Properties of a 110000 molecular weight protein in rat liver hnRNP particles.
abstract: Particles carrying heterogeneous nuclear RNA (30--40 S-particles) were isolated from rat liver nuclei and the particle proteins separated by sodium dodecylsulfate gel electrophoresis. Some properties of a 110000 molecular weight component (P 110/103) were studied in detail: (i) P 110/103 was labeled to a 4-5 times higher specific activity than the major particle proteins in the presence of [14C]-amino acids in vivo. (ii) In nuclei incubated with [3H]- or [32P]-nicotinamide adenine dinucleotide P 110/103 was labeled presumably by ADP-ribosylation. (iii) A protein with the same molecular weight as P 110/103 and isolated from the nuclear extract by affinity chromatography was phosphorylated in vitro.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis*; D008297:Male; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D009698:Nucleoproteins / Q000302:isolation & purification*; D010766:Phosphorylation; D012332:RNA, Heterogeneous Nuclear / Q000032:analysis*; D051381:Rats
chemical_list: D000596:Amino Acids; D009698:Nucleoproteins; D012332:RNA, Heterogeneous Nuclear; D009243:NAD