[Action of cyclophosphamide in a culture of rat postimplantation embryos].
The effect of cyclophosphamide (CPA) on the development of postimplantation rat embryos in vitro both in the homologous blood serum added with CPA and in the blood serum of the animals pretreated with CPA was studied. The latter method, unlike the former one, allows to take into account the metabolism of CPA in the organism of rats. It is proved by the similarity of teratogenic effects upon cultivation of the embryos in the blood serum of the rats pretreated with CPA and upon the effect of CPA on the embryos developing in vivo. The method for cultivation of the postimplantation embryos in vitro may be recommended for testing drugs subject to metabolic transformations.
D000818:Animals; D046508:Culture Techniques; D003520:Cyclophosphamide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian / Q000187:drug effects*; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D011247:Pregnancy; D051381:Rats; D013997:Time Factors
D003520:Cyclophosphamide
Izuchenie deĭstviia tsiklofosfamida v kul'ture postimplantatsionnykh zarodysheĭ krys.