Erythroid burst forming units (BFUE) grown from canine marrow and peripheral blood.
Studies of canine erythropoiesis in vitro have been limited to a relatively mature class of precursor that gives rise to erythroid colonies of 8-64 cells after 2-4 days in culture. In this report we provide data indicating that a second class of erythroid precursor that gives rise to clusters of colonies or bursts can be grown from both dog marrow and peripheral blood. Dog bursts reach maximum size after 7-14 days in culture and have an appearance characteristic of human erythroid bursts. Dog burst forming units, or BFUE, are low density cells (less than or equal to 1.065 g/ml) that can be separated from the majority of colony forming units, or CFUE, by density centrifugation.
D000818:Animals; D001773:Blood Cells / Q000166:cytology*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003114:Colony-Forming Units Assay; D004285:Dogs; D004920:Erythropoiesis; D004921:Erythropoietin / Q000494:pharmacology; D013997:Time Factors
D004921:Erythropoietin