title: tRNA-dimethylsulphate reaction: identification of 1,7-dimethylguanosine as a major product at high concentrations of methylating agent.
abstract: In the tRNA-dimethylsulphate reaction, 1,7-dimethylguanosine is formed at high concentrations of methylating agent. This dialkylated compound is recovered mainly in the form of derivative and has been identified by chromatographic and UV-spectral comparison with an authentic sample. The possible role of 1,7 alkylation of guanosine to carcinogenicity of alkylating reagents is advanced.
mesh_terms: D006151:Guanosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D008745:Methylation; D012343:RNA, Transfer; D013056:Spectrophotometry, Ultraviolet; D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids
chemical_list: D013463:Sulfuric Acid Esters; D013464:Sulfuric Acids; D006151:Guanosine; C031003:1,7-dimethylguanosine; D012343:RNA, Transfer; C007482:dimethyl sulfate