title: [Glutamin(asparagin)ase from Pseudomonas aurantiaca BKMB-548].
abstract: A method for isolation of glutamin (asparagin) ase from Pseudomonas aurantiaca BKMB-548 has been developed. The enzyme preparation is homogeneous during polyacrylamide gel electrophoresis (pH 7.5 and 7.0) with SDS. The pH-optima of the enzyme thermal stability and of the glutaminase activity are equal to 6.0-8.0. At higher pH values the asparaginase activity increases within the pH range of 4-9. The amino acid composition of glutamin(asparagin)ase has been determined.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001215:Asparaginase / Q000302:isolation & purification* / Q000378:metabolism; D005972:Glutaminase / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011549:Pseudomonas / Q000201:enzymology*
chemical_list: D000596:Amino Acids; D001215:Asparaginase; D005972:Glutaminase
vernacular_title: Glutamin(asparagin) aza iz Pseudomonas aurantiasa BKMB-548.