Simultaneous determination of phencyclidine and monohydroxylated metabolites in urine of man by gas chromatography-mass fragmentography with methane chemical ionization.
Phencyclidine and monohydroxy metabolites were measured in human urine using gas chromatography-mass fragmentography with methane chemical ionization. Samples were extracted either untreated or following acid hydrolysis, derivatized with heptafluorobutyric anhydride, separated on a 3% SE-30 column and analyzed by mass fragmentography. The assay was sensitive to ca. 0.01 microgram/ml for phencyclidine and ca. 0.05 microgram/ml for the metabolites. Urine samples from five human subjects enrolled in a methadone maintenance program who had ingested phencyclidine were analyzed. The phencyclidine concentration ranged from 0.3 to 23.7 microgram/ml. The concentrations of metabolites ranged from 0 to 1.8 microgram/ml. A new monohydroxy metabolite was detected in the samples, but its structure was not fully elucidated. The specificity of the assay was examined.
D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D010622:Phencyclidine / Q000378:metabolism / Q000652:urine*; D012016:Reference Values; D014116:Toxicology
D010622:Phencyclidine