Determination of cephalosporins in biological material by reversed-phase liquid column chromatography.
Seven cephalosporins (beta-lactam antibiotics), viz. cefazolin, cephalotin, cefoxitin, cefotaxime, cefamandole, cefuroxime and cefoperazone (T 1551) were determined in biological material. The compounds were extracted from acid-treated body fluids into chloroform--1-pentanol (3:1) and re-extracted into a small volume of an aqueous phase at Ph 7, which was injected into the chromatographic column. The chromatographic support was muBondapak C18 (10 micrometer) and the mobile phase was a mixture of 0.01 M acetate buffer (pH 4.8) and methanol or acetonitrile. Detection limits are about 50 ng/ml for extractions from 1 ml of serum and have permitted pharmacokinetic studies of the seven cephalosporins.
D002511:Cephalosporins / Q000008:administration & dosage / Q000097:blood*; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D012016:Reference Values; D013997:Time Factors
D002511:Cephalosporins