title: Structural relation of two S-100 proteins in bovine brain; subunit composition of S-100a protein.
abstract: Dodecyl sulfate/urea/polyacrylamide gel electrophoresis of S-100a protein, one of the two major components of the brain-specific S-100 protein, indicated the presence of two different subunits in the protein. These subunits (alpha and beta subunits) were purified from the aminoethylated protein by column chromatography on Sephadex G-75, and the purified subunits were subjected to analyses. The results have shown that S-100a protein is a dimer of alpha and beta subunits, with each subunit having a molecular weight of approximately 10500. Structural comparison of these subunits with the subunit of S-100b protein, the other component of S-100 protein consisting of two identical subunits with known amino acid sequence, has revealed that the beta subunit and the subunit of S-100b protein are identical, so that S-100a protein is related to S-100b protein by sharing one of the subunits as a common structural constituent.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015415:Biomarkers; D001923:Brain Chemistry; D002417:Cattle; D003488:Cyanogen Bromide; D046911:Macromolecular Substances; D008970:Molecular Weight; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000032:analysis; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D046911:Macromolecular Substances; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; C030564:S-100 calcium-binding protein alpha subunit; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D003488:Cyanogen Bromide