title: In vitro aging of human bone marrow derived stromal cells.
abstract: We have studied the in vitro cellular aging process in human bone marrow derived stromal cells. Two cell types, here called type I and type II, can be distinguished in the primary cell population. During subcultivation, resulting in aging in vitro, the type I cells gradually disappear, whereas the type II cells form an increasing fraction of the population. This observation is confirmed by cloning experiments, revealing that type I cells act as progenitor cells, giving rise to non-dividing type II cells. These results are best explained by the terminal differentiation theory as proposed by Martin et al.
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D006801:Humans; D008297:Male