Mitochondrial and cytosolic ATP/ADP ratios in isolated hepatocytes. A comparison of the digitonin method and the non-aqueous fractionation procedure.
The ratio of ATP content/ADP content in the mitochondrial matrix was found to be 2.07 +/- 0.21 and 2.26 +/- 0.22 as determined with six different preparations of isolated hepatocytes subfractionated with the digitonin and non-aqueous-fractionation procedures, respectively. In contrast, the mitochondrial matrix ATP/ADP determined with isolated haemoglobin-free perfused liver by using the non-aqueous-fractionation procedure was about 0.2, whereas the cytosolic values obtained with isolated cells and with the intact organ were similar. It is concluded that the relatively higher ATP/ADP ratio in the mitochondrial matrix of isolated hepatocytes represents a biochemical difference due to properties of the model rather than a methodological artifact.
D000244:Adenosine Diphosphate / Q000032:analysis*; D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D002251:Carbon Tetrachloride; D002458:Cell Fractionation / Q000379:methods; D003600:Cytosol / Q000032:analysis; D004072:Digitonin; D006536:Heptanes; D008099:Liver / Q000032:analysis* / Q000166:cytology; D008297:Male; D008930:Mitochondria, Liver / Q000032:analysis; D051381:Rats
D006536:Heptanes; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002251:Carbon Tetrachloride; D004072:Digitonin