title: CYBEST-CDMS; automated cell dispersion and monolayer smearing device for CYBEST.
abstract: The design and operational procedures of the cell dispersion and monolayer smearing (CSMS) machine for the CYBEST automated cytoscreening and image analysis system are described. The CYBEST-CDMS consists of two units: an autosyringing device for cells dispersion and an autosmearing device basically consisting of a modified commercial centrifuge. The operations of both devices are carried out with the samples in specially constructed centrifuge chamber units. The autosyringing device sequentially processes pairs of samples held in a rotor with a capacity of 12 centrifuge chamber units. Cell dispersion is achieved by syringing the samples 20 times each through 23-gauge needles. The use of Saccomanno solution instead of Ringer's solution as the final suspension medium prior to autosmearing has resulted in improved preparations. The CYBEST-CSMS machine largely fulfills one of the major requirements for automated cytology: the provision of samples with a sufficient number of isolated cells and a minimum number of clumped and/or overlapping cells
mesh_terms: D001322:Autoanalysis; D002469:Cell Separation / Q000295:instrumentation; D002498:Centrifugation; D002584:Cervix Uteri / Q000166:cytology*; D003581:Cytodiagnosis / Q000295:instrumentation*; D005260:Female; D006801:Humans; D013048:Specimen Handling / Q000295:instrumentation*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis; D014626:Vaginal Smears