Effect of cations on the linear dichroism and selective polarized light scattering of thylakoids.
The effect of cations on the linear dichroism (LD) and selective polarized light scattering of higher plant thylakoids was investigated. The results show that the major change in LD signal caused by the addition of cations is due to a scattering contribution most probably resulting from thylakoid stacking. However, minor changes in the LD signal also occur on the short wavelength side of the main LD band that persist even when a large proportion of the scattering change is eliminated by increasing the refractive index of the medium. The minor changes appear to be correlated with the cation-induced increase in variable fluorescence and resolution of the spectra at 77 K reveals that the changes in dichroism are due to LD bands of pigments associated with the light-harvesting complex.
D002118:Calcium / Q000494:pharmacology; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D008027:Light; D008274:Magnesium / Q000494:pharmacology; D009940:Organoids / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D008274:Magnesium; D002118:Calcium