Heritable translocation and dominant-lethal mutation induction with ethylene oxide in mice.
Ethylene oxide was studied for induction of dominant-lethal mutations and heritable translocations in male mice. The chemical was prepared in water and injected intraperitoneally. The dominant-lethal study was conducted using a single injection of 150 mg/kg (maximum tolerated dose); in the heritable translocation study males were injected daily on weekdays for 5 weeks with 60 or 30 mg/kg dose per day. Results clearly showed that ethylene oxide is effective in inducing dominant-lethal mutations and that the 4 stocks of untreated females used do not differ or may differ only slightly in the ability of their eggs to repair ethylene oxide-induced lesions in male germ cells. Increases in the frequencies of heritable translocations were also observed at the 2-dose levels. These frequencies did not deviate significantly from those expected on the basis of dose-square kinetics.
D000818:Animals; D004260:DNA Repair; D005027:Ethylene Oxide / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005799:Genes, Dominant / Q000187:drug effects; D005804:Genes, Lethal / Q000187:drug effects; D008297:Male; D051379:Mice; D009154:Mutation; D010063:Ovum / Q000201:enzymology; D014178:Translocation, Genetic / Q000187:drug effects*
D005027:Ethylene Oxide