A simple DNA-dependent fluorescence enhancement assay for cell number.
A DNA-dependent fluorescence enhancement assay using the dye Hoechst 33258 was developed to quantitate cell number in crude detergent lysates of cells. The assay, which uses a simple filter fluorometer, is sensitive enough to measure 1 x 10(4) cells without interference from mercaptans, culture media, and protein solutions. They assay can be done with freeze-thawed lysates. Fluorescence intensity values are linearly related to cell number in the range of 1 x 10(4) to 2.5 x 10(5) cells for L-929, BHK, and CHO-Kl cell lines.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000032:analysis*; D005260:Female; D007668:Kidney; D007739:L Cells / Q000032:analysis; D051379:Mice; D010053:Ovary; D013050:Spectrometry, Fluorescence / Q000379:methods
D004247:DNA