Human complement in the arachidonic acid transformation pathway in platelets.
Arachidonate-mediated release of 14C serotonin and thromboxane B2 (TXB2) is significantly enhanced in the presence of complement. Only purified complement components C5, C6, C7, C8, and C9 are required for this reactivity. No known activating mechanism of the classical or alternative pathway is required, nor is C3. In the absence of exogenously added complement, platelet membrane-bound complement components play an essential role in modulating arachidonate-mediated serotonin release. Incubation of platelet membranes with arachidonate and C5--C9 led to the production of dimers of the membrane attack complex (C5b--9) on the platelet surface. These macromolecular complexes were eluted from the platelet membrane and were identified physicochemically and morphologically. The possibility arises that C3 in association with C5--C9 is required for mobilization of the arachidonic acid from the phospholipid of the platelet membrane. Once the arachidonic acid is mobilized, C3 is no longer required, C5--C9 being sufficient to modulate this pathway leading to enhanced production of TXB2.
D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology*; D001792:Blood Platelets / Q000378:metabolism*; D002462:Cell Membrane / Q000276:immunology; D003176:Complement C3 / Q000276:immunology; D003181:Complement C4 / Q000276:immunology; D003182:Complement C5 / Q000378:metabolism; D003165:Complement System Proteins; D006801:Humans; D011817:Rabbits; D012701:Serotonin / Q000378:metabolism; D012709:Serum Albumin / Q000276:immunology; D013929:Thromboxane B2 / Q000378:metabolism
D001095:Arachidonic Acids; D003176:Complement C3; D003181:Complement C4; D003182:Complement C5; D012709:Serum Albumin; D012701:Serotonin; D013929:Thromboxane B2; D003165:Complement System Proteins