[Biological properties of Agrobacterium tumefaciens RNA-polymerase. I. Isolation of RNA-polymerase from Agrobacterium tumefaciens 8628].
RNA-polymerase is isolated from Agrobacterium tumefaciens 8628. When fractioning the protein extract by DEAE-cellulose, RNA-polymerase is separated into three fractions eluated with 0.15, 0.165, and 0.185 M KCI. Their electrophoresis in polyacrylamide gel shows that the composition of subunits in the second and third fractions is, in principle, similar to that of Escherichia coli RNA-polymerase, but the first fraction protein distribution pattern differs from he mentioned fractions. The data obtained may evidence for the presence of several RNA-polymerases forms in virulent strains of agrobacteria.
D002848:Chromatography, DEAE-Cellulose; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D012231:Rhizobium / Q000201:enzymology*
D012321:DNA-Directed RNA Polymerases
Biologicheskie svoĭstva RNK-polimerazy Agro bacterium tumefaciens. Soobshchenie. I. Vydelenienie RNK-polimerazy iz Agrobacterium tumefaciens 8628.