NADPH-dependent formation of aflatoxicol by mouse and rat lungs in vitro.
Homogenates and 10,000 g fractions obtained from lungs of rats (wister strain) and albino mice produced a fluorescent metabolite of aflatoxin B1, with similar Rf value and ultraviolet characteristics as aflatoxicol obtained from the liver of duck. This metabolite had Rf in ethyl acetate: chloroform (10:20 v/v) and in chloroform acetone: water (88:12:15 v/v) system similar to the standard aflatoxicol. In the absence of NADP, neither the homogenates nor the 10,000 g fractions produced this metabolite. It seems therefore that while the livers of rats and mice do not produce aflatoxicol, the lungs are capable of doing so.
D000348:Aflatoxins / Q000096:biosynthesis* / Q000378:metabolism; D000818:Animals; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D008297:Male; D051379:Mice; D009249:NADP / Q000494:pharmacology*; D051381:Rats
D000348:Aflatoxins; C012170:aflatoxicol; D009249:NADP