[DNA-dependent DNA polymerase induced by herpes virus papio (HVP) in producing cells].
A new DNA polymerase was found in the cells of suspension lymphoblastoid cultures, which produce lymphotropic baboon herpes virus (HVP). The enzyme was isolated in a partially purified form. In some properties the enzyme differs from other cellular DNA polymerases. The HVP-induced DNA polymerase has the molecular weight of 1,6 x 10(5) and sedimentation coefficient of about 8S. The enzyme is resistant to high salt concentrations and N-ethylmaleimide, but shows a pronounced sensitivity to phosphonoacetate. The enzyme effectively copies "activated" DNA and synthetic deoxyribohomopolymers. The attempts to detect the DNA polymerase activity in HVP virions were unsuccessful.
D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004790:Enzyme Induction; D006564:Herpesviridae / Q000201:enzymology*; D007700:Kinetics; D008214:Lymphocytes / Q000201:enzymology; D008970:Molecular Weight; D010215:Papio
D004259:DNA-Directed DNA Polymerase
Analiz DNK-zavisimoĭ DNK-polimerazy, indutsirovannoĭ virusom gerpesa pavianov(GVP) v produtsiruiushchikh kletkakh.