Cell electrophoresis for diagnostic purposes. II. Critical evaluation of conventional cytopherometry.
Determination of the electrophoretic mobility of test cells has been widely used in an attempt to detect so-called lymphokines in a laboratory test for cancer, but operational difficulties are inherent in conventional cytopherometers. This study therefore investigates the technical and operational aspects of cell electrophoresis, using the Zeiss cytopherometer; e.g. influence of electro-osmosis, focus uncertainty, movement due to convection and other sources of error. Implications and possible improvements in the test are discussed.
D002465:Cell Movement; D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis*; D055095:Optics and Photonics; D009995:Osmosis; D013997:Time Factors