Quantitative profiling of the metabolic cascade of arachidonic acid by capillary gas chromatography mass spectrometry.
The analytical approach described allows for the rapid screening and concurrent quantitative determination of all of the major metabolites of the cyclooxygenase pathway of arachidonic acid, including the primary prostaglandins PGE2, PGD2 and PGF2 alpha in addition to the stable end-products of short-lived prostacyclin and thromboxane A2 (6-keto-PGF1 alpha and TXB2, respectively), generated by incubating arachidonic acid with mouse peritoneal macrophage cells. After derivatization into the corresponding methyl ester-methoxime-TMS derivative, the extracted endogenous compounds were analysed by combined high efficiency glass capillary column chromatography and selected ion monitoring. Comparatively lower detection limits can be achieved with the methyl ester-butylboronate-TMS derivatives (e.g. 10 pg for 6-keto-PGF1 alpha). A brief account is also presented on a new type of mixed methyl ester-pentafluorobenzyloxime-butylboronate-TMS derivative of TXB2 and 5-keto-PGF1 alpha.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D001202:Ascitic Fluid / Q000166:cytology; D008401:Gas Chromatography-Mass Spectrometry; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D011457:Prostaglandins D / Q000378:metabolism; D011458:Prostaglandins E / Q000378:metabolism; D011460:Prostaglandins F / Q000378:metabolism; D013929:Thromboxane B2 / Q000378:metabolism; D014297:Trimethylsilyl Compounds / Q000032:analysis
D001095:Arachidonic Acids; D011457:Prostaglandins D; D011458:Prostaglandins E; D011460:Prostaglandins F; D014297:Trimethylsilyl Compounds; D013929:Thromboxane B2