title: Non-random distribution of aberrations and identification with C- and G-bandings of the position of breakage points on Muntjac chromosomes induced by mitomycin c, bromodeoxyuridine and hydroxylamine.
abstract: The analysis of chromosomes from muntjac after treatment of its lymphocyte cultures with 3 chemical mutagens having different base-pair affinities and modes of action, namely mitomycin C (MC), 5-bromodeoxyuridine (BUdR) and hydroxylamine hydrochloride (HA), with G- and C-band staining displayed non-random distribution of chemically specific damage points on them. The randomness of the involvement of each site on the chromosomes were examined by assuming an expected value calculated on the basis of its relative mitotic length. The observation revealed that a large fraction of MC-induced aberrations was preferentially located in the C-band positive constitutive heterochromatin, especially in the long "neck-like" centromeric region of the X-chromosome. On the chromosomal arms, the light G-bands were involved in aberrations either in proportion to or higher than that expected. When the cells were treated with BUdR, the dark G-bands on all the chromosomes of the complement were the preferred sites, displaying statistically significant higher numbers of aberrations. A single "hot-spot" for induced damage on 1 mid-q was also recorded. HA induced a very high frequency of damage in the secondary constriction regions of the chromosome pairs 1, X and Y2, and the frequency was slightly lower than this in the centromeres of 1, 2 and X chromosomes. The observation of specific distribution of damage points induced by the 3 chemicals lead to the suggestion that, though the effect of a chemical on chromosome segments depends on several factors, each being partially responsible for the end result, it is perhaps primarily depended by the chemical's base-pair affinity and mode of action.
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002875:Chromosomes / Q000187:drug effects* / Q000648:ultrastructure; D003670:Deer / Q000235:genetics*; D006898:Hydroxylamines / Q000494:pharmacology; D007621:Karyotyping; D008297:Male; D008937:Mitomycins / Q000494:pharmacology; D009153:Mutagens
chemical_list: D006898:Hydroxylamines; D008937:Mitomycins; D009153:Mutagens; D001973:Bromodeoxyuridine