Amino acid requirements of a Chlamydia trachomatis genital strain in McCoy cell cultures.
Amino acid requirements of a Chlamydia trachomatis were defined in McCoy cell monolayer cultures, depleted of their nutrient reserves by maintenance in Earle balanced salt solution for at least 2 days, and then treated at infection with 1 microgram of cycloheximide per ml, an inhibitor of host cell protein and deoxyribonucleic acid synthesis. Serum-free Waymouth MB 752/1 medium lacking one amino acid or a group of related amino acids was added to depleted cultures before infection. Eight of the amino acids normally present in MB 752/1 were essential for chlamydial growth, six were advantageous for growth, and five were nonessential.
D000596:Amino Acids / Q000494:pharmacology*; D002460:Cell Line; D002692:Chlamydia trachomatis / Q000254:growth & development*; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D005971:Glutamates / Q000494:pharmacology; D005973:Glutamine / Q000494:pharmacology; D006801:Humans; D002575:Uterine Cervicitis / Q000382:microbiology
D000596:Amino Acids; D003470:Culture Media; D005971:Glutamates; D005973:Glutamine; D003513:Cycloheximide