Post-fusion incompatibility in Physarum polycephalum. The involvement of DNA.
The lethal reaction following fusion of plasmodia of a sensitive and a killer strain of Physarum polycephalum could be prevented by the incorporation of 5-bromo-2'-deoxyuridine in both strains. This result suggests the involvement of transcription in the lethal reaction. Although under appropriate conditions fusion of the strains is not followed by a lethal reaction the fused plasmodium will behave as the killer strain after subculturing. At different times after fusion, DNA was isolated from a plasmodium in which visible lethal reaction was prevented and separated on CsCl gradients. Killer DNA remained intact but sensitive DNA was broken down, mainly from 8--11 h after fusion.
D001973:Bromodeoxyuridine / Q000378:metabolism; D004271:DNA, Fungal / Q000032:analysis / Q000502:physiology*; D010804:Physarum / Q000166:cytology / Q000502:physiology*
D004271:DNA, Fungal; D001973:Bromodeoxyuridine