title: Effect of centrifugation on fluorescence polarization of amniotic fluid.
abstract: Fluorescence polarization was measured on amniotic fluids of different gestational ages before and after centrifugation at relative centrifugal forces of 34 to 4955 x g. Centrifugation significantly increased the fluorescence polarization values over those of uncentrifuged amniotic fluids. The greatest increase was observed in the most "mature" fluids centrifuged at 34 to 230 x g. Almost without exception, use of centrifugal forces greater than 1239 x g did not further increase fluorescence polarization values.
mesh_terms: D000653:Amniotic Fluid / Q000032:analysis*; D002498:Centrifugation; D005260:Female; D006801:Humans; D008856:Microscopy, Fluorescence; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third