[Extraction of fecal lipids and identification of hydroxystearic acid by gas chromatography-mass spectrometry (GC/MS)].
Fatty acids and hydroxy fatty acids in the intestine are of interest because of their cathartic properties. We describe a method for extraction of lipids from freeze dried homogenized stools and identification of the hydroxy stearic acid in the fecal lipids pattern by GC-MS. Recovery of 90-95 % shows the efficacy of extraction . Quantitative data of fecal hydroxy stearic acid excretion in some normal and chronic diarrhea affected children are reported.
D002648:Child; D002908:Chronic Disease; D003967:Diarrhea / Q000378:metabolism; D005243:Feces / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D006880:Hydroxy Acids / Q000302:isolation & purification; D008055:Lipids / Q000302:isolation & purification*; D013229:Stearic Acids / Q000302:isolation & purification*
D006880:Hydroxy Acids; D008055:Lipids; D013229:Stearic Acids; C008083:10(9)-hydroxystearic acid
Estrazione dei lipidi fecali e determinazione dell'acido idrossi stearico mediante gas cromatograftaspettrometria di massa (GC/MS).