[Separation of human red blood cells of differing mean age: a method for small volumes of blood (author's transl)].
Human red blood cells have been separated into five fractions of differing mean ages by centrifugation through a density gradient of Ficoll-Triosil layers. The procedure is carried out on 0.3-0.6 ml packed erythrocytes, with good reproducibility and significant difference among the fractions. The separation is achieved using small ultracentrifuge rotors of common use, at 37,000 g. The enzymatic activity of glucose-6-phosphate dehydrogenase (G-6-PD) is estimated to test the difference in the mean age of the isolated fractions.
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006801:Humans
D005954:Glucosephosphate Dehydrogenase
Separazione di eritrociti umani di età diversa: metodo applicabile a piccoli volumi di sangue.