[Simple method for DNA fraction enrichment with unique genes].
The conditions for RNA--DNA molecular hybridization in 15% phenolic suspension, allowing to extend the temperature range of the R-loop formation and render the method insensitive to the nucleotide composition of RNA are proposed. The method described results in a 100-fold enrichment of the globin-gene content in a few days. The method was used for enrichment of rat DNA preparations with globin genes.
D000818:Animals; D001482:Base Composition; D004247:DNA / Q000378:metabolism*; D005796:Genes; D005914:Globins / Q000096:biosynthesis; D007700:Kinetics; D008722:Methods; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D051381:Rats; D013696:Temperature
D005914:Globins; D004247:DNA
Prostoi sposob obog a shcheniia praktsii DNK po soderzhaniiu unikal'nykh genov.