Determination of three main antileprosy drugs and their main metabolites in serum by high-performance liquid chromatography.
The simultaneous analysis of main antileprosy drugs such as 4,4'-diaminodiphenyl sulfone (DDS), clofazimine, rifampicin and their main metabolites in serum was examined by high-performance liquid chromatography using a muBondapak C18 column. When the drugs dissoluted from serum were developed by tetrahydrofuran-0.5% acetic acid (40:60), clofazimine and rifampicins could be analyzed separately. Apart from the mutual separation of water-soluble conjugates of DDS, the individual analysis of DDS, its main liposoluble metabolite and a few related sulfone compounds is possible when the drugs are first developed by acetonitrile-water (20:80). By the use of tetrahydrofuran-water (50:50) containing PIC B-5, the rapid measurement of clofazimine isolated from the other compounds is also possible.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002991:Clofazimine / Q000097:blood / Q000378:metabolism; D003622:Dapsone / Q000097:blood / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D007917:Leprostatic Agents / Q000097:blood* / Q000378:metabolism; D012016:Reference Values; D012293:Rifampin / Q000097:blood / Q000378:metabolism
D007917:Leprostatic Agents; D003622:Dapsone; D002991:Clofazimine; D012293:Rifampin