title: Production of protoplasts in different yeasts by mutanase.
abstract: Mutanase (Mutanase Novo) affects the high frequency production  of protoplasts in the following strains of yeast: Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Trichosporon pullulans, and Schwanniomyces alluvius. Regeneration frequencies varied with the strain used and ranged between 10 and 18%. This enzyme preparation appears to be a very useful means of obtaining protoplasts from a wide variety of yeasts currently being used for experimental purposes.
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D011523:Protoplasts; D013045:Species Specificity; D015003:Yeasts / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D006026:Glycoside Hydrolases; C021780:exo-1,3-alpha-glucanase