[Bimolecular protein-protein interactions in microcapsules].
It was shown that protein-protein interactions in microcapsules are possible. A mixture of two proteins at high concentrations was microencapsulated at acidic pH values of the aqueous phase under the following conditions: use of the double emulsification method, use of the inert filler--5% polyethylenimine for the formation of spherical non-aggregated microcapsules containing a small quantity of the protein in the membrane, and use of polycarbonate as a film-forming polymer. The kinetics of trypsin inhibition by soya inhibitor, trypsin autolysis and trypsinogen autoactivation were studied. Encapsulation did not influence the rate constant of the trypsin autolysis reaction and decreased to some extent the specific rate of trypsinogen autoactivation.
D004655:Emulsions; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D046911:Macromolecular Substances; D011506:Proteins / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000494:pharmacology; D014362:Trypsinogen / Q000378:metabolism
D004655:Emulsions; D046911:Macromolecular Substances; D011506:Proteins; D014362:Trypsinogen; D014360:Trypsin Inhibitor, Kunitz Soybean; D014357:Trypsin
Izuchenie bimolekuliarnykh belok-belkovykh vzaimodeĭstviĭ vnutri mikrokapsul.