Nuclear ribonucleoprotein particles from adenovirus infected Hela cells.
Heterogenous nuclear RNA-protein complexes (hnRNP) from adenovirus-2 (Ad-2) infected Hela cells contain most of the virus-specific RNA which is labeled in the nucleus during periods lasting from 45 seconds to 2 hours. Moreover, the percentage of RNA which is Ad-2 specific as monitored by filter hybridization increases progressively from early to late period where it accounts for as much as 50-60% of the labeled RNA. The Ad-2 sequences are found in heterogenous complexes sedimenting between 30 and 200 S the density of which in CsCl (rho approximately 1.39) as well as metrizamide (rho approximately 1.29) seems to be the same as that of the bulk particles. A more detailed analysis with restriction fragments shows that all regions of the Ad-2 genome are represented in these particles.
D000256:Adenoviridae; D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D005814:Genes, Viral; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D012332:RNA, Heterogeneous Nuclear / Q000032:analysis*; D012367:RNA, Viral / Q000378:metabolism*
D012332:RNA, Heterogeneous Nuclear; D012367:RNA, Viral