title: [Isolation and properties of NAD-dependent formate dehydrogenase from the yeast Candida methylica].
abstract: NAD-dependent formate dehydrogenase was purified from a cell-free extract of methanol-consuming yeast Candida methylica, using chromatography on DEAE-cellulose and gel-filtration on Sephadex G-200. The enzyme is electrophoretically homogeneous, consists of two identical subunits with molecular weight of 46,000 and is active within the pH range of 6-9; the Km values for NAD and formate are 1 . 10(-4) and 1,3 . 10(-2) M, respectively. Formate dehydrogenase is inhibited by p-chloromercurybenzoate, iodoacetate, dithionitrobenzoate and azide.
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D002175:Candida / Q000201:enzymology*; D005560:Formate Dehydrogenases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D009243:NAD
chemical_list: D009243:NAD; D005560:Formate Dehydrogenases; D000445:Aldehyde Oxidoreductases
vernacular_title: Vydelenie i svoĭstva NAD-zavisimoĭ formiatdegidrogenazy iz drozhzheĭ Candida methylica.