Highly purified mitochondria from rat brain prepared by phase partition.
Mitochondria and synaptosomes from adult rat forebrain can easily be separated by counter-current distribution in an aqueous two phase system composed of Dextran T500 and poly(ethylene glycol) 4000. Both particles may also be separated by a batch procedure in which the same phase system is used. Electron micrographs and enzymatic activities show a high purity of the mitochondria obtained from the dextran-rich lower phase. Electron micrographs and enzymatic activities also show that intact synaptosomes can be obtained from the poly(ethylene glycol)-rich upper phase. The mitochondria purified by this method show good ADP/O ratios, respiratory control ratios, and state 3 rates. Synaptosomes showed a state 2-state 3 transition with no recuperation to state 4.
D000110:Acetylcholinesterase / Q000032:analysis; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure*; D002458:Cell Fractionation / Q000379:methods; D003377:Countercurrent Distribution; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008297:Male; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure*; D010101:Oxygen Consumption; D051381:Rats; D013574:Synaptosomes / Q000648:ultrastructure
D007770:L-Lactate Dehydrogenase; D000110:Acetylcholinesterase