title: The effect of glucosone on the proliferation and energy metabolism of in vitro grown Ehrlich ascites tumor cells.
abstract: 1) Proliferation and energy metabolism of in vitro growth Ehrlich ascites tumor (EAT) cells in the presence of glucosone, (D-arabino-3.4.5.6-tetrahydroxy-2-oxo-hexanal) a competitive inhibitor of hexokinase, were studied. 2) Proliferation of the cells was completely inhibited by 2 mM glucosone without severely affecting viability (dye exclusion test). No phase specific arrest of cell growth was observed. 3) Incorporation of [14C]thymidine into an acid insoluble fraction of the cells decreases to 5% of the control within 8-10 h. Incorporation of [14C]leucine begins to slow down immediately after treatment with glucosone. 4) The inhibitor (2 mM) reduces the lactate production of the cells by 60%, respiration by about 20%; the ATP/ADP ratio slows down from 4.75 to 3.5. 5) The total inhibition of cell proliferation by 2 mM glucosone cannot be explained exclusively by inhibition of hexokinase activity and impairment of energy metabolism. Because of a lack of specificity, glucosone is not a suitable inhibitor for studies on the relationship between hexokinase activity and cell proliferation of tumor cells.
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000503:physiopathology*; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004734:Energy Metabolism / Q000187:drug effects*; D005260:Female; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D006593:Hexokinase / Q000037:antagonists & inhibitors; D007661:Ketoses / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D013936:Thymidine / Q000378:metabolism
chemical_list: D004273:DNA, Neoplasm; D007661:Ketoses; C017022:glucosone; D006593:Hexokinase; D005947:Glucose; D013936:Thymidine