Application of Ca2+-selective electrode for determination of Ca2+ efflux from smooth muscles into Ca2+-free physiological solution.
The Ca2+ efflux from smooth muscle into a Ca2+-free physiological solution was continuously monitored by using a Radiometer Ca2+-selective electrode and examined for its availability for determination of Ca2+ effluxed from the smooth muscle. The results show that the Ca2+ electrode used here is sufficiently sensitive to investigate Ca2+ efflux from the smooth muscle into the Ca2+-free solution. Further, this method is suitable for the continuous recording of free ionized Ca2+ effluxed from the smooth muscle into the Ca2+-free physiological solution.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004566:Electrodes; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008274:Magnesium; D008297:Male; D009130:Muscle, Smooth / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D051381:Rats; D012996:Solutions; D014325:Tromethamine
D012996:Solutions; D014325:Tromethamine; D008274:Magnesium; D002118:Calcium