Chemical-enzymatic synthesis of biologically active DNA fragments.
In this communication we report our recent investigations concerned the design and construction of synthetic functionally active DNA fragments: the structural gene for pentapeptide Leu-enkephalin and the double-stranded 93 base pairs long polynucleotide representing the promoter region of bacteriophage fd DNA. The ways for synthesis of modified promoter analogs carrying photosensitive residues of 5-bromodeoxyuridine in different positions of the chain are also considered.
D001482:Base Composition; D001483:Base Sequence; D003090:Coliphages; D004247:DNA / Q000138:chemical synthesis*; D004279:DNA, Viral / Q000138:chemical synthesis; D004743:Enkephalin, Leucine; D004745:Enkephalins / Q000235:genetics; D005796:Genes; D007202:Indicators and Reagents; D008722:Methods; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009876:Operon
D004279:DNA, Viral; D004745:Enkephalins; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004743:Enkephalin, Leucine; D004247:DNA