Affinity chromatography of arterial lysosomal cholesterol ester hydrolase.
The glycoproteic nature of rabbit aortic lysosomal cholesterol ester hydrolase has been demonstrated by affinity chromatography on Concanavalin A-Sepharose. After chromatography, the enzyme lacks synthesizing activity. This activity is restored by addition of deactivated lysosomes containing more endogenous cholesterol. On the other hand, a hypothesis for the activation role of bis(monoacylglyceryl) phosphate is suggested.
D000818:Animals; D001011:Aorta / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002788:Cholesterol Esters / Q000096:biosynthesis; D002846:Chromatography, Affinity; D003208:Concanavalin A; D004789:Enzyme Activation; D008246:Lysophospholipids; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D050178:Monoglycerides; D010712:Phosphatidic Acids / Q000502:physiology; D011817:Rabbits; D002787:Sterol Esterase / Q000378:metabolism*
D002788:Cholesterol Esters; D008246:Lysophospholipids; D050178:Monoglycerides; D010712:Phosphatidic Acids; C012786:bis(monoacylglyceryl)phosphate; D003208:Concanavalin A; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase