Biosynthesis of glycoproteins by membranes of Acer pseudoplatanus. Incorporation of mannose and N-acetylglucosamine.
Membrane preparations from Acer pseudoplatanus suspension cultures were demonstrated to incorporate radioactivity from GDP-[U-14C]mannose and UDP-N-acetyl-[6-(3)H]glucosamine into high-molecular-weight polymers characterized as glycoprotein. From 20 to 25% of the 14C was incorporated as fucose with the remainder as mannose, whereas 90% of the 3H was incorporated as N-acetylglucosamine with the remainder as N-acetylgalactosamine. Pronase digestion yielded radioactive glycopeptides that were separated into four fractions by gel-permeation chromatography and paper electrophoresis. The isolated glycopeptides differed in molecular weight and isotopes incorporated, as well as in amino-acid and monosaccharide composition. The membrane preparation also incorporated radioactivity from the added nucleotides into chloroform/methanol (2:1, v/v)- and chloroform/methanol/water (10:10:3, by vol.)-soluble lipids, and into an insoluble pellet.
D000117:Acetylglucosamine / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D002850:Chromatography, Gel; D005944:Glucosamine / Q000031:analogs & derivatives*; D006020:Glycopeptides / Q000032:analysis; D006023:Glycoproteins / Q000096:biosynthesis*; D008358:Mannose / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis*; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D010944:Plants / Q000378:metabolism*
D000596:Amino Acids; D006020:Glycopeptides; D006023:Glycoproteins; D008565:Membrane Proteins; D009005:Monosaccharides; D005944:Glucosamine; D008358:Mannose; D000117:Acetylglucosamine