A semi-automated technique for assessing the microdistribution of 239 Pu deposited in bone.
A semi-automated analytical procedure is described for the localisation of 239 Pu in bone. The method is based on the use of a Quantimet 720B Image Analyser for the analysis of neutron-induced autoradiographs, and may be applied to most tissues with fissionable radionuclides. Compared with former methods the present technique offers improvements in accuracy and a considerable saving on time and manpower. These savings arise mainly from the ability of the Quantimet to be used for the automatic detection of the fission-fragment tracks and to operate directly on the neutron-induced autoradiograph without recourse to photography. Results are presented for the distribution of 239 Pu in the ilia of CBA mice at 24 h post-injection.
D000818:Animals; D001322:Autoanalysis / Q000379:methods; D001345:Autoradiography; D001842:Bone and Bones / Q000378:metabolism*; D005260:Female; D051379:Mice; D008808:Mice, Inbred CBA; D009502:Neutrons; D011005:Plutonium / Q000378:metabolism*; D013997:Time Factors
D011005:Plutonium