Drug resistance studies on intraspecific hybridomas.
P388 mouse leukemia lines, one sensitive (P388/S) and the other resistance (P388/R) to vincristine (VCR), cultured in vitro, were hybridized with polyethylene glycol (PEG). A thymidine kinase-deficient mutant (TK-) was isolated from the sensitive line, and a hypoxanthine-guanine phosphoribosyltransferase-deficient mutant (HPRT-) from the resistant line. The hybrid line grows slower than the mutants. The modal chromosome numbers are: TK- = 38, HPRT- = 40, hybrid = 69 (72). The TK- cells contain a large metacentric marker which is missing from the HPRT- cells. Hybrid cells are as resistant to VCR as the P388/R and HPRT- cells.
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002460:Cell Line; D004351:Drug Resistance; D006822:Hybrid Cells / Q000187:drug effects; D007620:Karyometry; D007941:Leukemia P388 / Q000188:drug therapy* / Q000235:genetics; D007942:Leukemia, Experimental / Q000188:drug therapy*; D051379:Mice; D008811:Mice, Inbred DBA; D009154:Mutation; D014750:Vincristine / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D014750:Vincristine