Determination of D-penicillamine in serum by fluorescence derivatization and liquid column chromatography.
A simple and fast method for the determination of D-penicillamine in serum is described. The analysis is based o a fluorescence derivatization of the sulfhydryl group combined with a reversed-phase liquid chromatograhic separation and fluorescence detection. Before derivatization the serum proteins are precipitated with ethanol and removed by centrifugation. As derivatizing agent 5-dimethylaminonaphthaline-1-sulfonylaziridine is used which reacts selectively with thiols under defined reaction conditions. The detection limit is in the pmol range; 50-300 ng of D-penicillamine can be determined with a relative standard deviation of 7-8%. Thus the method permits a simple determination of D-penicillamine in serum at therapeutic levels.
D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D010396:Penicillamine / Q000097:blood*; D012016:Reference Values; D013050:Spectrometry, Fluorescence
D010396:Penicillamine