[Fractionation of brain phosphopeptides].
Anion-exchange chromatography was used to fractionate the brain phosphopeptides preparations obtained by the common method and the method modified by the author. The data of a comparative analysis show that in fractions obtained from our preparation the content of amide nitrogen as well as of phosphate residues is higher than in the analogous preparations of Ledig and Mandel. Changes are found in the spectrum of the phosphopeptides fractions isolated during incubation of the brain tissue homogenate under aerobic conditions (1 h; 37 degrees C). It is shown that the amount of phosphopeptide fractions decreases after incubation, and the total content of nitrogen, content of amide nitrogen and phosphorus in eluted fractions undergo redistribution.
D000332:Aerobiosis; D000577:Amides / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002852:Chromatography, Ion Exchange / Q000379:methods; D010748:Phosphopeptides / Q000032:analysis*
D000577:Amides; D010748:Phosphopeptides
Fraktsionirovanie fosfopeptidov golovnogo mozga.