Purification of dihydropteridine reductase from human platelets.
Dihydropteridine reductase was purified approximately 1,700-fold from human outdated blood platelets. Two forms of the enzyme, A and B, were resolved. They have the same Km values for 2-amino-6,7,-dimethyl-4-hydroxydihydropteridine (46 microM vs 49 microM), but the A form has a Km for NADH that is two times higher than that of the B form (20 microM vs 9 microM).
D001792:Blood Platelets / Q000201:enzymology*; D002845:Chromatography; D004093:Dihydropteridine Reductase / Q000302:isolation & purification*; D006801:Humans; D007700:Kinetics; D009243:NAD / Q000032:analysis; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification*
D009243:NAD; D004093:Dihydropteridine Reductase; D009247:NADH, NADPH Oxidoreductases