[Development of a gentamicin producer under different culture conditions studied by a method of differential centrifugation of the mycelium in a saccharose density gradient].
The method of differential centrifugation in the sucrose density gradient (SDG) enabled one to trace the changes in the development of the seed and fermentation mycelium of the gentamicin-producing organism. Correlation between gentamicin distribution in the SDG and the culture productivity was found. It was shown that the culture grown under the optimal aeration and agitation conditions was characterized by formation of higher amounts of the mycelium in the 5th and 6th layers of the SDG. Such mycelium was more productive than that from the other SDG layers. The most productive 48-hour seed culture had the more significant part of the mycelium in the 3rd layer of the SDG. When such a culture had the more significant part of the mycelium in the 3rd layer of the SDG. When such a culture was used as the seed material, the activity of the fermentation broth was the highest. The method of differential centrifugation in the SDG provides determination of the culture productivity by the volumes of the fermentation mycelium in the 5th and 6th layers or the seed mycelium in the 3rd layer of the SDG.
D002499:Centrifugation, Density Gradient / Q000379:methods; D003470:Culture Media; D005285:Fermentation; D005839:Gentamicins / Q000096:biosynthesis*; D008847:Micromonospora / Q000254:growth & development*
D003470:Culture Media; D005839:Gentamicins
Izuchenie razvitiia produtsenta gentamitsina v razlichnykh usloviiakh kul'tivirovaniia s pomoshch'iu metoda differentsial'nogo tsentrifugirovaniia mitseliia v gradiente plotnosti sakharozy.