DNA damage in human skin fibroblasts exposed to UVA light used in clinical PUVA treatment.
Human skin fibroblasts were irradiated with a clinically used UVA light source. The doses (1.1 and 3 J/cm2) were similar to those reaching the dermis during clinical PUVA treatment of psoriasis. DNA strand breaks, as determined by alkaline elution, were formed in a dose-dependent way and disappeared within 1 hr of postincubation at 37 degrees C. These findings have clinical implications since UVA-induced DNA damage may be accompanied by mutagenic and tumor promoting effects.
D004247:DNA / Q000528:radiation effects*; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D011701:PUVA Therapy / Q000009:adverse effects*; D010778:Photochemotherapy / Q000009:adverse effects*; D012867:Skin / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects
D004247:DNA