[Determination of O-agglutinins against Yersinia enterocolitica by the indirect hemagglutination test (author's transl)].
O-agglutinins against Yersinia enterocolitica serovar 03 and serovar 09 in serum specimens were determined by the indirect (passive) hemagglutination test and the Widal technique. The hemagglutination test was carried out in microtitre plates with formalized and sensitized turkey erythrocytes. Cell wall antigens (lipopolysaccharides) were prepared by the phenol-water method. The indirect hemagglutination test had a high sensitivity and specificity. Moreover, this test has the advantage of being easier to set up and read than the bacteria agglutination. No unspecific reactions were observed.
D000373:Agglutinins / Q000032:analysis*; D000907:Antibodies, Bacterial / Q000032:analysis; D000918:Antibody Specificity; D006386:Hemagglutination Tests / Q000379:methods; D015009:Yersinia Infections / Q000175:diagnosis / Q000276:immunology*
D000373:Agglutinins; D000907:Antibodies, Bacterial
Zum Nachweis von O-Agglutininen gegen Yersinia enterocolitica im indirekten Hämagglutinationstest.