Spectrophotometric analysis for fluorouracil in biological fluids.
Fluorouracil (5-fluorouracil; 5-FU) is a monoanion at pH = 10 and forms an ion-pair complex with a quaternary alkylammonium ion. De Leenheer and co-workers [2] reported that the ion-pair complexes might be quantitatively extracted from aqueous solutions into organic solvents, and they spectrophotometrically determined the concentration of 5-FU. But as their method used column chromatography, it was cumbersome and time-consuming. Here we present a more rapid chemical assay of 5-FU by modifying the principle of their method. The applicability of this method was shown by determining the plasma and tissue levels of 5-FU in mice.
D000818:Animals; D001826:Body Fluids / Q000032:analysis; D005472:Fluorouracil / Q000032:analysis* / Q000097:blood; D051379:Mice; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D005472:Fluorouracil