Detection of lysozyme production using modified technique as an aid for the identification of pathogenic staphylococci causing some animal and human diseases.
Lysozyme production is an essential character of the potentially pathogenic staphylococci. In the present work 88 strains of animal origin and 40 strains of human origin were tested. Of 103 strains isolated from pathogenic cases of human and animal origin 89 (86.4%) were lysozyme producers and 86 (83.5%) were coagulase positive. Out of 75 strains isolated from pathogenic cases of animal origin 75 (100%) were lysozyme producers and 71 (94.6%) were coagulase positive. On the other hand out of 28 strains isolated from pathogenic human cases 14 (50%) were lysozyme producers and 15 (53.6%) were coagulase positive. This indicates that lysozyme production could be a better index of pathogenic staphylococci than the coagulase measurement specially in cases of animal origin strains. The method used in this work for the determination of the lysozyme production seems to be a simple one if compared with other used methods.
D000818:Animals; D002020:Buffaloes; D002417:Cattle; D003030:Coagulase / Q000096:biosynthesis; D005260:Female; D006041:Goats; D006801:Humans; D008414:Mastitis, Bovine / Q000382:microbiology; D008722:Methods; D008892:Milk / Q000382:microbiology; D009113:Muramidase / Q000032:analysis / Q000096:biosynthesis*; D013203:Staphylococcal Infections / Q000382:microbiology / Q000662:veterinary; D013210:Staphylococcus / Q000201:enzymology / Q000302:isolation & purification / Q000472:pathogenicity*; D014556:Urine / Q000382:microbiology
D003030:Coagulase; D009113:Muramidase