Analysis of nuclear accumulation of influenza NP antigen in von Magnus virus-infected cells.
When 1-5C-4 cells were infected with von Magnus virus derived from influenza A/RI/5+ virus by successive undiluted passages in chick embryos, virus-specific proteins were synthesized but production of infectious virus was inhibited. In these cells the synthesis of viral RNA was suppressed and the nucleoprotein (NP) antigen was found predominantly in the nucleus in contrast to standard virus-infected cells in which the antigen was distributed throughout the whole cell. The intracellular location and migration of NP were determined by isotope labeling and sucrose gradient centrifugation of subcellular fractions. In standard virus-infected cell NP polypeptide was present predominantly in the cytoplasm in the form of viral ribonucleoprotein (RNP) and intranuclear RNP was detected in reduced amounts. In contrast, in von Magnus virus-infected cells NP polypeptide was present predominantly in the nucleus in a nonassembled, soluble from and the amount of cytoplasmic RNP was considerably reduced. After short-pulse labeling NP was detected exclusively in the cytoplasm in a soluble form and after a chase a large proportion of such soluble NP was seen in the nucleus. It is suggested that a large proportion of the NP synthesized in von Magnus virus-infected cells in not assembled into cytoplasmic RNP because of the lack of available RNA and the NP migrated into the nucleus and remained there.
D000956:Antigens, Viral / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis*; D002999:Clone Cells; D006801:Humans; D007251:Influenza, Human / Q000473:pathology*; D009698:Nucleoproteins / Q000032:analysis / Q000276:immunology*; D009975:Orthomyxoviridae / Q000254:growth & development; D010452:Peptide Biosynthesis; D012261:Ribonucleoproteins / Q000032:analysis; D013045:Species Specificity
D000956:Antigens, Viral; D009698:Nucleoproteins; D012261:Ribonucleoproteins