Effect of actinomycin-D on the staining of whole cells with acridine orange.
Flow cytofluorometric techniques have been used to investigate the interference of actinomycin-D with the staining of acridine orange in whole cells. The results show that reduction in fluorescence intensity will only occur at actinomycin-D concentrations greater than 10(3) ng/ml in our system, providing the drug is washed out prior to staining.
D000165:Acridine Orange; D000818:Animals; D001854:Bone Marrow Cells; D002460:Cell Line; D003609:Dactinomycin; D007700:Kinetics; D051379:Mice; D013050:Spectrometry, Fluorescence
D003609:Dactinomycin; D000165:Acridine Orange