Simultaneous determination of cyclandelate and its metabolite in human plasma by capillary column gas-liquid chromatography.
A method was developed for the simultaneous determination of cyclandelate and mandelic acid concentrations in plasma, involving extraction from plasma followed by trimethylsilylation and chromatography of the derivatives on a glass capillary column with hydrogen flame-ionization detection. Calibration graphs were linear down to at least 20 microgram/ml for each substance. The precision was excellent with a pooled relative standard deviation of 6.3% and 6.4% for cyclandelate and mandelic acid serum samples, respectively. Concentrations below 500 ng/ml of each substance could be detected in human plasma. The method was developed for use in bioavailability and metabolism studies.
D000818:Animals; D002849:Chromatography, Gas; D003495:Cyclandelate / Q000097:blood*; D006801:Humans; D008333:Mandelic Acids / Q000097:blood*; D012016:Reference Values
D008333:Mandelic Acids; D003495:Cyclandelate