Cardiac catecholamine stores after cardiac sympathectomy, 6-OHDA, and cardiac denervation.
Sympathetic nerves are known to reach the heart via classical vagal pathways. Bilateral cervicothoracic sympathectomy (CS) might be expected therefore to leave significant amounts of releasable catecholamines in the myocardium if the vagi are left intact. Cardiac responses to tyramine (500 micrograms intracoronary) were compared in animals with CS, extrinsic cardiac denervation (CD), and chemical sympathectomy with 6-hydroxydopamine (6-OHDA). Dogs were placed on cardiopulmonary bypass and isovolumic pressures were recorded from the heart. In control animals, tyramine caused profound increases in rate (58%) and isovolumic pressures (86-170%). Ten CS animals demonstrated responses indistinguishable from control animals. Four CD and four 6-OHDA animals showed no response to tyramine. Cervical vagal stimulation after atropine in the CS animals produced tachycardia. Histofluorescent studies demonstrated the presence of significant numbers of cardiac adrenergic fibers in this group. The normal tyramine responsiveness of the CS animals was attributed to 1) significant numbers of vagal adrenergic fibers remaining in the heart after cervicothoracic sympathectomy, and 2) denervation supersensitivity, rendering the heart very responsive to the catecholamine that remains.
D000818:Animals; D016275:Atrial Function; D001285:Atropine / Q000494:pharmacology; D002395:Catecholamines / Q000032:analysis*; D003714:Denervation; D004285:Dogs; D005260:Female; D006321:Heart / Q000187:drug effects / Q000294:innervation*; D006892:Hydroxydopamines / Q000494:pharmacology*; D008297:Male; D008856:Microscopy, Fluorescence; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000032:analysis*; D014439:Tyramine / Q000494:pharmacology; D014630:Vagus Nerve / Q000187:drug effects* / Q000502:physiology; D016276:Ventricular Function
D002395:Catecholamines; D006892:Hydroxydopamines; D001285:Atropine; D014439:Tyramine