Determination of carbohydrates in urine by high-performance liquid chromatography and optical activity detection.
Improvements in a detector for liquid chromatography based on optical activity of the components have led to a detectability of 100 ng. This allows the simultaneous determination of six naturally occurring carbohydrates in 100-microliters samples of human urine, which is injected directly except for a simple deionization step. The reproducibility and reliability of this method should allow better insight into the relation between urinary sugars and physiological conditions.
D002241:Carbohydrates / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004187:Disaccharides / Q000652:urine; D006801:Humans; D009005:Monosaccharides / Q000652:urine; D012016:Reference Values; D014312:Trisaccharides / Q000652:urine
D002241:Carbohydrates; D004187:Disaccharides; D009005:Monosaccharides; D014312:Trisaccharides