On the standardisation of the direct spectrophotometric bilirubin determination. Influence of the albumin source and the molar bilirubin: albumin ratio.
The influence of different albumin solutions and of the molar bilirubin/albumin ratio on the bilirubin concentration determined by the method for Hertz et al. was studied. There is good agreement between the true-bilirubin concentrations and those obtained by the Hertz method for bilirubin-HSA and bilirubin-serum solutions. Moreover the observed concentration is scarcely influenced by the molar ratio. Bilirubin standards in HSA-solutions can thus be used to standardise this method. Errors are introduced by preparing standards in BSA-solutions as the absorption spectrum of bilirubin-BSA is different from that of bilirubin-HSA.
D001663:Bilirubin / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012015:Reference Standards; D012709:Serum Albumin / Q000592:standards*; D012710:Serum Albumin, Bovine / Q000592:standards; D012996:Solutions; D013053:Spectrophotometry
D012709:Serum Albumin; D012996:Solutions; D012710:Serum Albumin, Bovine; D001663:Bilirubin