title: Red blood cell preservation in protein-poor media. 2. Studies of changes in red cell shape during storage.
abstract: Red cells stored for up to 35 days have been studied by scanning electron microscopy. Formation of spicules occurred under all storage conditions in CPD and CPD-adenine whole blood, CPD-adenine red cell concentrate, and saline-adenine-glucose red cell concentrate. The number of cells with normal or near normal shape was larger if the blood was stored as a moderately concentrated suspension in saline-adenine-glucose medium or in autologous plasma as compared to the storage as whole blood. The occurrence of echinocytes during storage at +4 degrees C was not correlated to red cell ATP. It was shown that a drastic reduction of ATP leads to an increased formation of both spherocytes and echinocytes. The change of red cell shape during storage at +4 degrees C thus can be due to two processes, one unrelated to ATP and another related to ATP. Why storage as red cell concentrate is superior is not fully understood.
mesh_terms: D001800:Blood Specimen Collection; D002951:Citrates; D004912:Erythrocytes / Q000648:ultrastructure*; D005947:Glucose; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010710:Phosphates; D013997:Time Factors; D014021:Tissue Preservation
chemical_list: D002951:Citrates; D010710:Phosphates; D005947:Glucose