Properties of soluble DNA polymerase from sera of hepatitis B virus carriers.
A soluble DNA polymerase was purified 8,000-fold from hepatitis B surface antigen positive serum. The molecular weight of the enzyme by gel filtration was about 1.60 X 10(5), the sedimentation coefficient was 5.5S, the apparent Km for dTTP was 4 micrometer, the optimum pH in the presence of Mg2+ was 9.2, and the pl was 4.7. The enzyme was found in HBsAg-positive sera and required an external primer for activity. The properties of the DNA polymerase were different from hepatitis B virus particle enzyme and from vertebrate and bacterial DNA polymerases. The prevalence of this enzyme did not correlate with HBeAg or particle DNA polymerase in HBsAg-positive sera.
D002353:Carrier State / Q000201:enzymology*; D004259:DNA-Directed DNA Polymerase / Q000032:analysis / Q000097:blood* / Q000302:isolation & purification; D006509:Hepatitis B / Q000201:enzymology* / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006801:Humans; D007526:Isoelectric Point; D008970:Molecular Weight; D012995:Solubility
D006514:Hepatitis B Surface Antigens; D004259:DNA-Directed DNA Polymerase