Synthesis of phosphatidyl-dCMP in human lymphocytes and its enhancement by chlorpromazine.
When phytohemagglutinin-stimulated lymphocytes are labelled with [3H]deoxycytidine (dCyd), a compound soluble in organic solvents is formed. Several analytical procedures such as thin-layer chromatography and alkaline hydrolysis of the product obtained after labeling of stimulated lymphocytes with [14C]glycerol and [3H]dCyd, suggest that the product synthesized is phosphatidyl-dCMP. This compound is synthesized in much smaller amounts than when permeabilized lymphocytes and [3H]dCTP as a precursor are used. The synthesis of phosphatidyl-dCMP is highly enhanced by chlorpromazine, phosphatidic acid and arachidonic acid, whereas addition of inositol diminishes the amount of detected phosphatidyl-dCMP. It is suggested that this compound would be a precursor of phosphatidylinositol.
D002746:Chlorpromazine / Q000494:pharmacology*; D003841:Deoxycytidine / Q000097:blood; D003843:Deoxycytidine Monophosphate / Q000031:analogs & derivatives / Q000096:biosynthesis*; D003845:Deoxycytosine Nucleotides / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000096:biosynthesis; D010835:Phytohemagglutinins
D003845:Deoxycytosine Nucleotides; D010712:Phosphatidic Acids; D010835:Phytohemagglutinins; C020461:phosphatidyl-dCMP; D003841:Deoxycytidine; D003843:Deoxycytidine Monophosphate; D002746:Chlorpromazine