title: Prolongation of effect of asparaginase by implantation in polyacrylamide in rats.
abstract: Asparaginase was immobilized in spherical polyacrylamide microparticles, which were inserted in polyacrylamide gel for implantation in rats. Intraperitoneal implantation of this gel depressed systemic L-asparagine for the same duration as was achieved with microparticles, i.e., 8-10 days. The duration of the effect was prolonged up to about 25 days when the gel with the enzyme was implanted subcutaneously on the back of the rat. In both cases, a collagenous capsule formed around the gel, eventually preventing contact with the circulation. The effect of a second implantation was of the same magnitude as the first one.
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D001215:Asparaginase / Q000008:administration & dosage / Q000494:pharmacology*; D001216:Asparagine / Q000097:blood*; D003864:Depression, Chemical; D004343:Drug Implants; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D051381:Rats; D013997:Time Factors; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D001215:Asparaginase; D001216:Asparagine; D004343:Drug Implants; D000099166:Polyacrylamides