Karyotypic analysis of human ovarian carcinoma cells cloned in short term agar culture.
We report detailed chromosome banding analysis of six cases of ovarian adenocarcinoma cultured 24--96 hr in a recently developed bioassay for clonogenic tumor cells. The results of G and C banding revealed a variety of chromosome changes including the observation of a simple deletion for a portion of the long arm of chromosome #6, del(6)(pter leads to q15--21:), in tumor cells from four of six patients. Our study indicates that short-term agar culture can provide a valuable tool in the study of tumor cell karyology. A comparison of our results with previously published studies suggests that in addition to alterations of chromosome #1, the deletion of 6q may represent a characteristic chromosomal aberration in ovarian carcinoma.
D000230:Adenocarcinoma / Q000235:genetics*; D002478:Cells, Cultured; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002877:Chromosomes, Human / Q000648:ultrastructure*; D002906:Chromosomes, Human, 6-12 and X / Q000648:ultrastructure; D002999:Clone Cells; D005260:Female; D006801:Humans; D007621:Karyotyping; D010051:Ovarian Neoplasms / Q000235:genetics*