Monoclonal antibodies to blood group antigens on ruminant red cells.
Hybridomas were made between NSI/1-Ag-4-1 mouse myeloma cells and spleen cells Balb/c mice immunized with red cells from sheep (SRBC), goats (GRBC) and cattle (CRBC). The monoclonal antibodies thus produced were tested against a panel of RBC from 80 sheep, 20 goats and 100 cattle of known blood types. One antibody reacted with all RBC from all 3 species, two with all GRBC and SRBC but not CRBC, one was specific for SRBC and two others for CRBC. In addition two monoclonal antibodies were reactive in the B blood group system and two in the FV system of cattle. IgM and IgG concentration in ascite fluids reached up to 8 mg/ml. It is concluded that the hybridoma technique has considerable potential for the production of blood-typing reagents.
D000818:Animals; D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens / Q000276:immunology*; D002417:Cattle; D002999:Clone Cells / Q000276:immunology; D006041:Goats; D006168:Guinea Pigs; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D012756:Sheep
D000911:Antibodies, Monoclonal; D001789:Blood Group Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M