Identification and determination of 2-thiothiazolidine-4-carboxylic acid in urine of workers exposed to carbon disulfide.
A compound was isolated from the urine of workers exposed to carbon disulfide during the production of rayon. The compound was identified as 2-thiothiazolidine-4-carboxylic acid (TTCA) by GC/MS and NMR. A HPLC method for the quantitative determination of TTCA was elaborated and applied to urine samples. The method allowed detection of TTCA concentrations in urine as low as 5 X 10(-7) M. No TTCA was detected in urine of workers occupationally exposed to organic solvents other than CS2. High concentrations of TTCA (upto 32 X 10(-5) M) were shown to be present in urine of spinners exposed to CS2 concentrations of approximately 100 mg/m3. It is suggested that the assay of urinary TTCA is suitable for detection of occupational exposure to CS2.
D002246:Carbon Disulfide / Q000378:metabolism*; D002264:Carboxylic Acids / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004781:Environmental Exposure; D006801:Humans; D009787:Occupational Medicine; D013438:Sulfhydryl Compounds / Q000652:urine*; D013783:Textile Industry; D013844:Thiazoles / Q000652:urine*; D053778:Thiazolidines
D002264:Carboxylic Acids; D013438:Sulfhydryl Compounds; D013844:Thiazoles; D053778:Thiazolidines; C031677:2-thioxo-4-thiazolidinecarboxylic acid; C005104:2-mercaptothiazoline; D002246:Carbon Disulfide