Preparation of monospecific antiserum to lupin nodule glutamate dehydrogenase.
Immunization of rabbit, using biochemically homogeneous glutamate dehydrogenase, proved to be unsuitable to produce monospecific antiserum. The presence of traces of contaminating immunogen (undetected by physiochemical methods) induced the production of other antibodies. Procedures for rigorously establishing monospecificity of antisera and a technique for preparation of monospecific antiserum, using immunologically impure antigens, are described.
D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D003600:Cytosol / Q000201:enzymology; D005969:Glutamate Dehydrogenase / Q000276:immunology*; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007527:Isoenzymes / Q000276:immunology; D010944:Plants / Q000201:enzymology*; D011817:Rabbits / Q000276:immunology
D000911:Antibodies, Monoclonal; D007106:Immune Sera; D007527:Isoenzymes; D005969:Glutamate Dehydrogenase