[Study of lipid composition of mitochondrial and endoplasmic membranes by horizontal flow chromatography].
A new modification of a highly sensitive horizontal flow chromatography procedure for the study of lipid composition of intracellular membranes is proposed. This modification employs application of thin layers of homogeneous silicagel particles by precipitation from diluted water suspensions in a cell of original construction, which allows to obtain by a single analysis distinct lipid fractions having similar chromatographic mobility. Using this modification, the major forms of lysophosphatides, i. e. lysophosphatidylcholines, lysophosphatidylethanolamines, lysocardiolipids, lysophosphatidic acids, lysophosphatidylserines, etc., and their diacyl analogs were isolated from mitochondrial and reticulum membranes and identified. The contents of mono-, di- and triglycerides, free fatty acids, cholesterol and its esters were determined.
D000818:Animals; D002784:Cholesterol / Q000032:analysis; D002788:Cholesterol Esters / Q000032:analysis; D002845:Chromatography / Q000295:instrumentation / Q000379:methods; D004721:Endoplasmic Reticulum / Q000032:analysis*; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005989:Glycerides / Q000032:analysis; D007425:Intracellular Membranes / Q000032:analysis*; D008563:Membrane Lipids / Q000032:analysis*; D008928:Mitochondria / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis*; D011817:Rabbits; D051381:Rats
D002788:Cholesterol Esters; D005230:Fatty Acids, Nonesterified; D005989:Glycerides; D008563:Membrane Lipids; D010743:Phospholipids; D002784:Cholesterol
Analiz lipidnogo sostava mitokhondrial'nykh i endoplazmaticheskikh membran s pomoshch'iu metoda protochnoĩ gorizontal'noĩ khromatografii.