Effects of 6-chloro-6-deoxysugars on glucose oxidation in rat spermatozoa.
6-Chloro-6-deoxyfructose or 6-chloro-6-deoxyglucitol (greater than 90 mumol/kg/day), 6-chloro-6-deoxyglucose or 6-chloro-6-deoxymannose (greater than 120 mumol/kg/day) and 6-chloro-6-deoxygalactose (greater than 300 mumol/kg/day) all had an antifertility action in the male rat when given by mouth. Spermatozoa from the infertile rats were unable to oxidize glucose. This effect was always produced by a lower dose than the antifertility effect and the threshold dose for the 2 effects varied in a parallel fashion between the different 6-chloro-6-deoxysugars. Glucose oxidation appeared to be inhibited at the triose phosphate isomerase or glyceraldehyde 3-phosphate dehydrogenase reaction. These effects of 6-chloro-6-deoxysugars are similar to those of alpha-chlorohydrin. The activities of the glycolytic enzymes were greatly in excess of the observed flux through the pathway and high concentrations of glucose 6-phosphate and triose phosphate accumulated in rat spermatozoa incubated with 2 mM-D-glucose.
D000818:Animals; D003272:Contraceptive Agents, Male / Q000494:pharmacology*; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005298:Fertility / Q000187:drug effects; D005632:Fructose / Q000031:analogs & derivatives / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism*
D003272:Contraceptive Agents, Male; D003837:Deoxy Sugars; D005632:Fructose; C021163:6-chloro-6-deoxyglucose; C021162:6-chloro-6-deoxyfructose; D003847:Deoxyglucose; D005947:Glucose