A simple method for in vitro studies with rodent urinary bladders.
A method was developed for the in vitro study of rodent urinary bladders. The method consists of everting and distending the urinary bladder in a manner to allow exposure of the luminal surface of the urothelium during in vitro incubation while maintaining the integrity of the structure and morphology of the bladder. A technique for selectively removing the urothelium with SDS buffer for biochemical analysis was described. Incorporation of [3H]leucine into urothelial protein was linear over a 4 h period in the presence of tissue culture medium, but no significant incorporation occurred when urine was used as incubation medium. Autoradiography indicated the [3H]leucine incorporation was almost exclusively in the urothelial cells with essentially no incorporation by cells below the tunica propria.
D000818:Animals; D004848:Epithelium / Q000033:anatomy & histology / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009924:Organ Culture Techniques / Q000379:methods*; D012967:Sodium Dodecyl Sulfate; D001743:Urinary Bladder / Q000033:anatomy & histology / Q000502:physiology*
D012967:Sodium Dodecyl Sulfate; D007930:Leucine