Isolation and characterization of alpaca growth hormone.
A simple and mild procedure is described for the isolation of pituitary growth hormone from alpaca glands. It involves extractions in buffer at pH 8.7 and gel filtration in Sephadex G-75. an average yield of 1 mg purified product may be obtained from 1 g fresh alpaca pituitary glands. The alpaca hormone was examined for homogeneity by SDS-polyacrylamide gel electrophoresis, gel electro-focusing and end-group analyses. It is a protein composed of a single polypeptide chain with phenylalanine at both ends. The C-terminal sequence is -Ala-Phe. The molecular weight is approximately 21 700 and the amino acid composition very similar to that observed in equine growth hormone.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001188:Artiodactyla / Q000378:metabolism*; D002161:Camelids, New World / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D013006:Growth Hormone / Q000032:analysis* / Q000302:isolation & purification; D007526:Isoelectric Point; D008297:Male; D008970:Molecular Weight
D000596:Amino Acids; D013006:Growth Hormone