A new method for the isolation of aminoacylase from mammalian kidneys.
Aminoacylase (E.C. 3.5.1.14) was isolated from the kidneys of different mammalian species (horse, cattle, rabbit and pig) by extracting the organ with water and subjecting the extract to heat treatment at 70 degrees C for 10 min, then, after having removed denatured proteins by fractionating those remaining in the solution by ammonium sulfate. The enzyme obtained in this way can either be used directly for practical purposes (e.g. preparation of immobilized aminoacylase) or further purified by chromatography. For the further purification of porcine kidney aminoacylase we applied a combination of ion exchange chromatography and gel filtration.
D000581:Amidohydrolases / Q000302:isolation & purification*; D000645:Ammonium Sulfate; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006736:Horses; D006358:Hot Temperature; D007668:Kidney / Q000201:enzymology*; D011489:Protein Denaturation; D011817:Rabbits; D013552:Swine
D000581:Amidohydrolases; C019672:aminoacylase I; D000645:Ammonium Sulfate