Purification of an alkaline phosphatase-lipoprotein-X complex.
An alkaline phosphatase isoenzyme was observed in an abnormal position in the agar-agarose gel pattern of sera from several patients suffering from intrahepatic and extrahepatic cholestasis. We purified this isoenzyme, by gel filtration and affinity chromatography, from the serum of a patient suffering from extrahepatic cholestasis. Analysis demonstrated an alkaline phosphatase-lipoprotein-X complex with a relative molecular mass of at least 669,000. We discuss the interpretation of alkaline phosphatase isoenzyme patterns produced by different techniques.
D000368:Aged; D000469:Alkaline Phosphatase / Q000097:blood / Q000302:isolation & purification*; D002779:Cholestasis / Q000097:blood / Q000201:enzymology; D002850:Chromatography, Gel; D004587:Electrophoresis, Agar Gel; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007527:Isoenzymes / Q000097:blood; D008073:Lipoprotein-X / Q000302:isolation & purification*; D008297:Male; D014462:Ultrafiltration
D007527:Isoenzymes; D008073:Lipoprotein-X; D000469:Alkaline Phosphatase