Influence of the host cell on the genomic and subgenomic RNA content of defective-interfering influenza virus.
Clonally derived stocks of defective-interfering (DI) influenza virus prepared by chick embryo fibroblast (CEF) cells were all deficient in genomic RNAs 1, 2 and 3 but had different patterns of subgenomic RNAs. A single passage at high multiplicity in L cells altered the pattern of genomic RNAs independently of the subgenomic species, while in BHK cells the opposite situation prevailed. Therefore, there is no simple relationship in DI influenza virus between the loss of genomic RNA segments and the presence of subgenomic RNAs.
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D006224:Cricetinae; D003673:Defective Viruses / Q000032:analysis*; D005814:Genes, Viral; D009980:Influenza A virus / Q000032:analysis* / Q000235:genetics / Q000254:growth & development; D007739:L Cells; D051379:Mice; D012367:RNA, Viral / Q000032:analysis*; D014762:Viral Interference
D012367:RNA, Viral