Purine metabolism of erythrocytes preserved in adenine, adenine-inosine, and adenine-guanosine supplemented media.
Four different media for erythrocyte preservation have been compared by studying 16 variables mainly describing the purine metabolism and glycolysis of the erythrocyte. The concentrations of the additives in the erythrocyte suspensions were as follows: adenine, 0.25 mmol/l; adenine, 0.75 mmol/l; adenine, 0.25 and inosine, 0.50 mmol/l; and adenine, 0.25 and guanosine, 0.50 mmol/l. Evaluated from the concentrations of glycerate 2,3-bisphosphate and purine nucleoside triphosphates, the medium with adenine-guanosine was superior to the others. In this medium with adenine-guanosine was superior to the others. In this medium the guanosine was rapidly split to guanine, which was slowly used for nucleotide synthesis or deaminated to xanthine. Differences between ATP and GTP in their reactivity with the two enzymes phosphofructose kinase seems to explain the beneficial effect of guanosine on preservation of erythrocytes.
D000225:Adenine; D001793:Blood Preservation; D004912:Erythrocytes / Q000378:metabolism; D006151:Guanosine; D006801:Humans; D007288:Inosine; D011687:Purines / Q000378:metabolism*
D011687:Purines; D006151:Guanosine; D007288:Inosine; D000225:Adenine; C030985:purine