[Role of lysosome permeability disorders in the mechanism of the low-temperature inhibition of protein biosynthesis in the rat liver].
The effect of the supernatants of lysosomes subjected to a rapid or slow freezing and thawing on the protein-synthesizing activity of the rat liver postmitochondrial supernatant (S15) has been studied. It has been found that the addition of supernatants obtained after a slow freezing or thawing to a cell-free system results in a significant inhibition of 14C-leucine incorporation that is in a good agreement with their RNase activity. However, the RNase activity of lysosomal supernatants or Triton-lysate appears to be higher that the inhibitory effect on protein biosynthesis. The damaging effect of supernatants obtained after 2-hour storage at 2-4 degrees C of the frozen-thawed lysosomes has not virtually changed.
D000818:Animals; D002474:Cell-Free System; D005615:Freezing; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism*; D008297:Male; D010539:Permeability; D014176:Protein Biosynthesis; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism
D012260:Ribonucleases; D007930:Leucine
Rol' narusheniia pronitsaemosti lizosom v mekhanizme nizkotemperaturnogo ugneteniia biosinteza belkov v pecheni krys.