Effect of beta-adrenergic stimulation by isoprenaline on proliferation and differentation of mouse bone marrow cells in vivo.
In CFW mice exposed to 550 R of X-rays 75 min after administration of isoprenaline (IPR) the number of endogenous spleen colonies was increased from 2.15 +/- 0.4 to 33 +/- 3.78 per spleen. In animals injected with hydroxyurea 15 min after IPR injection the number of endogenous spleen colonies did not exceed the normal values, giving evidence for rapid triggering of pluripotent stem cells from G0 into S phase. No changes in the number of colonies were observed when IPR was administered 30 min after or 16 hr before irradiation. However, 16 hr after administration of IPR, the maximal proliferative response of bone marrow cells (determined by autoradiography with 3H-TdR) was noted. An increase in erythropoietic activity (measured by 59Fe incorporation into erythrocytes) was also observed in animals treated with IPR. The above data indicate that the beta-adrenergic stimulation by IPR results in the increase of proliferative activity of hemopoietic stem cells and their enhanced differentiation towards erythropoiesis.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004920:Erythropoiesis / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D051379:Mice; D013154:Spleen / Q000166:cytology; D013268:Stimulation, Chemical
D007545:Isoproterenol