Schultz-Dale reaction in mouse trachea.
A method was developed to induce contraction of immunologically sensitized mouse trachea by antigen (Schultz-Dale reaction). The response was mediated by immunoglobulin (Ig) E antibody directed against either the hapten DNP, the hapten carrier conjugate DNP-keyhole limpet hemocyanin (KLH), or the unmodified carrier KLH. Tracheal contractions were elicited by DNP-KLH, KLH, or DNP-bovine serum albumin (BSA) but not by DNP or BSA alone. This procedure represents a useful index of in vitro anaphylaxis in mouse airway smooth muscle.
D000707:Anaphylaxis / Q000503:physiopathology*; D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D000941:Antigens; D004140:Dinitrophenols / Q000276:immunology; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000276:immunology*; D014132:Trachea / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D004140:Dinitrophenols; D006433:Hemocyanins