Brain tubulin microheterogeneity in the mouse during development and aging.
Mouse brain tubulin was analyzed on isoelectric focusing gels. High-resolution gels utilizing Bio-Rad ampholytes (pH 4--6) revealed 5--6 bands in the region corresponding to the alpha-subunit of tubulin and 10 or more for the beta-subunit. The same general banding pattern was observed regardless of the method of preparation of the tubulin. Two species prominent in the brains of immature mice, alpha 6 and beta 2, virtually disappeared during maturation, while species beta 6 to beta 10 appeared. No significant changes from the mature pattern were seen during aging (examined at 12, 23, and 30 months of age).
D000375:Aging; D000818:Animals; D001921:Brain / Q000254:growth & development*; D001923:Brain Chemistry; D007525:Isoelectric Focusing; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D014404:Tubulin / Q000032:analysis*
D046911:Macromolecular Substances; D014404:Tubulin