High-pressure liquid chromatographic analysis of sennosides A and B purgative drugs.
Procedures are described for the analysis of the main anthraquinone glycosides of senna powder, senna fruit tablets, and sennoside tablets by high-pressure liquid chromatography (HPLC). In one HPLC analysis, TLC was used to separate the glycosides prior to elution on a strong anion-exchange column with 0.1 M ammonium nitrate solution (pH 9.0) as the mobile phase. In another HPLC analysis, separation was effected using a weak anion-exchange column with 0.1 M ammonium nitrate solution (pH 5.7A) as the mobile phase.
D000880:Anthraquinones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004639:Emetics / Q000032:analysis; D012676:Senna Extract / Q000032:analysis*; D000081226:Sennosides
D000880:Anthraquinones; D004639:Emetics; D000081226:Sennosides; D012676:Senna Extract