Determination of calcium ions effluxed from smooth muscle into Ca2+-free physiological solution by means of Ca2+-selective electrode.
In order to measure Ca2+ efflux from the smooth muscle into a Ca2+-free physiological solution, the method using a Radiometer Ca2+-sensitive electrode was applied and compared with the method using an atomic absorption spectrophotometer to examine its usefulness. The results obtained show that the Ca2+ electrode used here can accurately and continuously monitor free calcium ions effluxed from the smooth muscle into a Ca2+-free solution. The Ca2+-sensitive electrode used here is a useful tool to investigate Ca2+ efflux from the smooth muscle into a Ca2+-free physiological solution.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D004566:Electrodes; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism; D007811:Lanthanum / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000378:metabolism*; D009638:Norepinephrine / Q000494:pharmacology; D011817:Rabbits; D013054:Spectrophotometry, Atomic
D007811:Lanthanum; D000109:Acetylcholine; D002118:Calcium; D009638:Norepinephrine