The energy dispersive X-ray microanalysis technique in the study of fluids and tissues of the brain and the inner ear.
The temporal bone from the fetal and adult mouse and brain from fetal and adult rat were cryosectioned in a conventional cryostat and -30 degrees C using 16 micrometers thick sections. The elemental composition was analyzed in the fluid-filled spaces of the membranous labyrinth and ventricular cavities of the brain. After drying these spaces containing small crystal-like aggregations of material which morphologically differed depending on their elemental composition. This changed during embryonic maturation. The qualitative X-ray analytical microscopy seems to be a reliable method to follow changes in the elemental composition of fluids.
D000375:Aging; D000818:Animals; D001921:Brain / Q000196:embryology / Q000254:growth & development* / Q000648:ultrastructure; D001923:Brain Chemistry; D007758:Ear, Inner / Q000032:analysis / Q000196:embryology / Q000254:growth & development*; D004577:Electron Probe Microanalysis / Q000379:methods; D005260:Female; D006168:Guinea Pigs; D051379:Mice; D008815:Mice, Inbred Strains; D008855:Microscopy, Electron, Scanning / Q000379:methods; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains