Metabolism of isoniazid and free hydrazine formation in isolated rat hepatocytes.
Isoniazid (INH) was incubated with isolated rat hepatocytes, and the amounts of acetyl-INH, monoacetylhydrazine, diacetylhydrazine and hydrazine formed were determined by gas chromatography-mass spectrometry (GC-MS). Subsequent analyses of kinetic parameters of metabolic pathways and enzymological study of rat liver homogenate revealed that hydrazine was formed preferentially from INH, catalyzed by soluble hydrolytic enzymes.
D000818:Animals; D006834:Hydrazines / Q000378:metabolism*; D066298:In Vitro Techniques; D007538:Isoniazid / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D006834:Hydrazines; D007538:Isoniazid