Identification of phospholipid as an essential part of bovine vitamin K-dependent carboxylase.
Vitamin K-dependent carboxylase from bovine liver contains phospholipid (primarily phosphatidylcholine), which is essential for its in vitro activity. Sepharose-bound carboxylase can be depleted of phospholipids, either by washing the enzyme with detergents or by phospholipase treatment. The enzyme can be reconstituted by adding mixed micelles of phosphatidylcholine and cholate to the Sepharose-bound proteins.
D000818:Animals; D019735:Carbon-Carbon Ligases; D002417:Cattle; D003902:Detergents / Q000494:pharmacology; D007700:Kinetics; D008025:Ligases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D010740:Phospholipases; D010743:Phospholipids / Q000032:analysis* / Q000494:pharmacology; D014812:Vitamin K / Q000494:pharmacology*
D003902:Detergents; D010743:Phospholipids; D014812:Vitamin K; D010740:Phospholipases; D008025:Ligases; D019735:Carbon-Carbon Ligases; C020660:glutamyl carboxylase