Cytogenetics of neoplasia of Xiphophorus.
Certain laboratory hybrid Xiphophorus fish develop heritable neoplasia. Neoplasia can also be induced in some hybrids by treatment with carcinogens. In either case the neoplasms are mediated by a "'tumor gene," that is normally controlled by linked or nonlinked "regulating genes" or both. Light and electron microscopic studies on these two types of tumors reveal important differences. The heritable tumor is a compact mass of tissue formed of densely packed melanized melanocytes often with nuclear pockets and projections. A large number of cells undergoing a process of nuclear fragmentation has been observed. The induced tumors, on the other hand, contain fewer melanized melanocytes and show a comparative increase in the amount of connective tissue. The nuclear pockets and projections commonly found in the melanocytes of the heritable tumors are not present in the melanocytes of the induced melanoma. The most important difference observed was the presence of different types of structural chromosome aberrations in the induced tumor and a total absence in the heritable ones. These anomalies seem to be epiphenomenal, and the real genetic change is subchromosomal.
D000818:Animals; D002869:Chromosome Aberrations; D003433:Crosses, Genetic; D005393:Fish Diseases / Q000235:genetics*; D005399:Fishes; D005809:Genes, Regulator; D007621:Karyotyping; D008545:Melanoma / Q000139:chemically induced / Q000235:genetics* / Q000648:ultrastructure; D008677:Metaphase; D008770:Methylnitrosourea; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics
D008770:Methylnitrosourea