In vivo and in vitro localization of Leishmania within macrophage phagolysosomes: use of colloidal gold as a lysosomal label.
The interaction of Leishmania with lysosomes within macrophages in vivo has been investigated. Lysosomes labeled with colloidal gold in vivo fused with phagocytic vacuoles containing Leishmania amastigotes within the macrophages of infected footpad tissue of BALB/c mice. This localization of Leishmania within macrophage phagolysosomes in vivo is the first confirmation for any obligate intracellulaire protozoon that parasite-lysosome interactions in vitro occur in vivo.
D000818:Animals; D003102:Colloids; D006046:Gold; D007891:Leishmania / Q000502:physiology*; D008247:Lysosomes / Q000469:parasitology* / Q000648:ultrastructure; D008264:Macrophages / Q000469:parasitology* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D009940:Organoids / Q000469:parasitology*; D010587:Phagocytosis; D014617:Vacuoles / Q000469:parasitology* / Q000648:ultrastructure
D003102:Colloids; D006046:Gold