A human parathyroid carcinoma that produces parathyroid hormone: long term maintenance in tissue culture.
Parathyroid carcinoma cells from a pulmonary metastasis of a patient with a serum Ca of 17 mg/dl and an immunoreactive parathyroid hormone (PTH) of 6.4 ng eq bovine (b) PTH/ml (normal 40--400 pg/ml) have been maintained in tissue culture for more than 2 1/2 years. The cells secrete PTH into the culture media that 1) during immunoassay dilutes in parallel to human hyperparathyroid serum, 2) has a molecular weight similar to intact highly purified bPTH, and 3) stimulates bone resorption in a manner that is equivalent and additive to synthetic bPTH-(1--34).
D000328:Adult; D001681:Biological Assay; D001862:Bone Resorption / Q000187:drug effects; D002118:Calcium / Q000097:blood; D046508:Culture Techniques; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000556:secondary; D010281:Parathyroid Hormone / Q000378:metabolism* / Q000494:pharmacology; D010282:Parathyroid Neoplasms / Q000378:metabolism* / Q000473:pathology
D010281:Parathyroid Hormone; D002118:Calcium