[Thermal sensitivity of aldolase isoenzymes in the organs of cattle, sheep and swine].
During the investigation of homogenates from organs of cattle, sheep and swine (a heart, a kidney, a pancreas, a liver, a thigh muscle, a spleen, a lung) the highest activity of aldolase was proved in the bodily muscles, whereas in the other organs it did not show great differences. Thus it is determined as an ubiquitous enzyme in all these kinds of farm animals. After heating at 61 degrees C for 15 min. aldolase from the bodily muscles of cattle, sheep and swine, showed a high degree of thermostability (residual activity of 86 = 92%), whereas in the parenchymatous organs the enzyme was considerably thermosensitive (residual activity of 15.2--48.4%). The liver isoenzyme of aldolase showed the highest degree of thermosensitivity and had a residual activity of hardly 8--12%. A viewpoint is endorsed, namely that on that basis can be differentiated some pathological processes in the musculature from the pathological processes in the parenchymatous organs.
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D004355:Drug Stability; D004789:Enzyme Activation; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D006358:Hot Temperature; D007527:Isoenzymes / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*; D013997:Time Factors
D007527:Isoenzymes; D005634:Fructose-Bisphosphate Aldolase
Termochuvstvitelnost na aldolaznite izoenzimi v organite na goveda, ovtse i svine.