[Cooxidation of potassium ferrocyanide and o-dianisidine by hydrogen peroxide catalyzed by horseradish peroxidase. Substrate-substrate activation].
The kinetics of cooxidation of potassium ferrocyanide and o-dianisidine by hydrogen peroxide catalyzed by horseradish peroxidase were studied. The peroxidation of potassium ferrocyanide is activated by o-dianisidine. A scheme illustrating the direct involvement of the enzyme in substrate-substrate activation is proposed. A method for determination of the rate constants of the first electron transfer from o-dianisidine to peroxidase (i. e. reduction of peroxidase E1 to E2) and of the constants for o-dianisidine binding by E1 was developed.
D001560:Benzidines / Q000494:pharmacology*; D003962:Dianisidine / Q000494:pharmacology*; D004579:Electron Transport; D005295:Ferrocyanides / Q000494:pharmacology*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006861:Hydrogen Peroxide; D007700:Kinetics; D008433:Mathematics; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*
D001560:Benzidines; D005295:Ferrocyanides; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D010544:Peroxidases; C031835:potassium ferrocyanide; D003962:Dianisidine
Sovmestnoe okislenie ferrotsianina kaliia i o-dianizidina perekis'iu vodoroda, kataliziruemoe peroksidazoĭ iz khrena. Substrat-substratnaia aktivatsiia.