Estimation of the number of monoclonal hybridomas in a cell fusion experiment. Effect of post-fusion cell dilution on hybridoma survival.
A practical method is described for the estimation of the number of monoclonal hybridomas in a cell fusion experiment as a function of the percent of culture dishes showing hybridoma growth. Our method is based on the Poisson probability model. A justification for the method is included. The application of this model to our experimental results indicates that the probability of hybridoma survival decreases with post-fusion cell dilution even in the presence of a constant number of feeder cells.
D000818:Animals; D002459:Cell Fusion; D002470:Cell Survival; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D006822:Hybrid Cells / Q000276:immunology; D006825:Hybridomas / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors