Inhibition of in vitro keratinization of chick embryo skin cultured with hepatectomized rat serum.
In the present study organ cultures of 6 day-old chick embryo matatarsal skin were used to determine the effect of hepatectomized rat serum in modulating epithelial differentiation. Six day-old chick embryo skin, maintained in organ culture for 6-7 days with a medium containing adult rat serum, was seem to undergo differentiation and keratinization faster then in vivo. Epidermis cultured for 6-7 days with a medium containing partially hepatectomized rat serum does not differentiate and the squamous layer is absent. Studies with 3H-cystine show a sharp decrease in the labelled amino-acids incorporation in the cultures added with serum obtained after partial hepatectomy.
D000818:Animals; D001769:Blood; D002454:Cell Differentiation; D002642:Chick Embryo; D003553:Cystine / Q000378:metabolism; D006498:Hepatectomy; D008297:Male; D009924:Organ Culture Techniques; D051381:Rats; D011919:Rats, Inbred Strains; D012867:Skin / Q000166:cytology* / Q000196:embryology
D003553:Cystine