Fractionation of human serum lipoproteins by high performance liquid chromatography. I.
Immunologically pure lipoprotein fractions of human serum were subjected to high performance liquid chromatography (HPLC) on a combination system of aqueous gel permeation columns. Each lipoprotein fraction appeared at a distinct elution volume as a single homogeneous peak. Elution was carried out with 0.1 M Tris-HCl buffer solution, pH 7.4, or 0.15 M NaCl solution and no significant differences in elution volume and elution pattern were found. The lipoprotein mixtures (total lipoprotein fraction d less than 1.210) of healthy and pathological subjects were subjected to HPLC and showed characteristic elution patterns which could be of diagnostic significance.
D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002914:Chylomicrons; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D008075:Lipoproteins, HDL / Q000097:blood; D053439:Lipoproteins, HDL2; D053440:Lipoproteins, HDL3; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D010743:Phospholipids / Q000097:blood; D014280:Triglycerides / Q000097:blood
D002914:Chylomicrons; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D053439:Lipoproteins, HDL2; D053440:Lipoproteins, HDL3; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol