The metabolism of prostaglandin E2 is decreased by sulfinpyrazone in isolated hamster lungs.
The metabolism of prostaglandin E2 (PGE2) was decreased in isolated male hamster lungs, when sulfinpyrazone was infused into the pulmonary circulation. After pulmonary injection of 20 nmol of 14C-PGE2 the amount of 15-keto-metabolites of PGE2 was in the effluent from control lungs 4.0 +/- 0.5 nmol (mean +/- SEM) and in those from 20 mu M and 100 mu M sulfinpyrazone treated lungs 1.9 +/- 0.2 nmol (2P Less Than 0.01 compared to the control) and 1.7 +/- 0.4 nmol (2P Less Than 0.01), respectively. The amount of unmetabolized PGE2 was correspondingly increased in the effluent by sulfinpyrazone. The rate of efflux of the radioactivity from the lungs was increased by sulfinpyrazone. After injection of 10 nmol of 14C-PGE2 into the pulmonary circulation half of the injected radioactivity appeared in the effluent in 30 +/- 4 sec in control and in 15 +/- 0.7 sec (2P Less Than 0.01) in 20 mu M sulfinpyrazone experiments. Sulfinpyrazone had no effect on the activity of 15-hydroxyprostaglandin dehydrogenase in the 100.000 g supernatant fraction of homogenized hamster lungs. Thus the decreased metabolism of PGE2 in the pulmonary circulation of hamster lungs is obviously not due to the inhibition of 15-hydroxyprostaglandin dehydrogenase. A more likely explanation seems to be the decreased uptake of PGE2 into the lung cells.
D000818:Animals; D006224:Cricetinae; D015232:Dinoprostone / Q000031:analogs & derivatives*; D066298:In Vitro Techniques; D007700:Kinetics; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D011458:Prostaglandins E / Q000378:metabolism*; D011652:Pulmonary Circulation; D013442:Sulfinpyrazone / Q000494:pharmacology*
D011458:Prostaglandins E; C026346:15-ketoprostaglandin E2; D015232:Dinoprostone; D013442:Sulfinpyrazone