Degradation of unsaturated fatty acids in peroxisomes. Existence of a 2,4-dienoyl-CoA reductase pathway.
1. There are two 2,4-dienoyl-CoA reductases (EC 1.3.1.-) (formerly called 4-enoyl-CoA reductase, Eur. J. Biochem. (1978) 91, 533-544) in rat liver, one in mitochondria and another one in peroxisomes. 2. Treatment of rats with clofibrate increases the activities of the 2,4-dienoyl-CoA reductases in both cell organelles. 3. Isolated peroxisomes metabolize 4-cis-decenoyl-CoA, a metabolite of linoleic acid, via the 2,4-dienoyl-CoA reductase pathway. 4. From these results it is concluded that peroxisomes have the enzymatic apparatus necessary for the degradation of unsaturated fatty acids.
D000818:Animals; D002994:Clofibrate / Q000494:pharmacology; D044943:Fatty Acid Desaturases / Q000302:isolation & purification / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008830:Microbodies / Q000187:drug effects / Q000201:enzymology*; D009940:Organoids / Q000201:enzymology*; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D005231:Fatty Acids, Unsaturated; D044943:Fatty Acid Desaturases; D002994:Clofibrate