Serologic and biochemical identification of minor histocompatibility (H-4) antigens.
Mouse alloantiserum capable of detecting minor histocompatibility antigens has been produced. Cytotoxic antibody to products of the H-4 locus was produced by reciprocal immunization of minor H loci congenic strains B10 (H-4a) and B10.129(21M) (H-4b), with mitogen-stimulated lymphocytes and epidermal cells. Absorption studies suggested antibodies to more than 1 specificity. The antibodies cross-react with products of other minor H loci. Alloantiserum to the H-4 gene product precipitates a glycoprotein with a m.w. of approximately 45,000 from radiolabeled Con A-stimulated lymph node cells.
D000042:Absorption; D000818:Animals; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D002874:Chromosome Mapping; D003429:Cross Reactions; D006649:Histocompatibility Antigens / Q000235:genetics; D007106:Immune Sera / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL
D006649:Histocompatibility Antigens; D007106:Immune Sera