On the immunological detection of X-ray induced DNA damage.
Denatured calf-thymus DNA was X-irradiated, coupled to methylated bovine serum albumin and injected into rabbits to study the possible formation of specific antibodies. The serological activity was tested by a modified Farr-test, a micro complement binding reaction and by caesium chloride isopycnic ultracentrifugation. It was found with all assays that the immunological reaction was mainly due to unspecific DNA-binding and only a very small amount to radiation products. It seems, therefore, that this approach is not suitable for the analytical investigation of DNA damage produced by ionizing radiation.
D000818:Animals; D002500:Centrifugation, Isopycnic; D004247:DNA / Q000528:radiation effects*; D007118:Immunoassay / Q000379:methods; D011817:Rabbits; D011833:Radiation Injuries, Experimental / Q000378:metabolism
D004247:DNA