Cytogenetic analysis and flow cytometric DNA measurement of a human tumor with pronounced hypodiploidy.
A case of malignant choroid plexus papilloma of the brain with severe hypodiploidy is presented. The hypodiploidy was estimated by means of flow cytometric measurements of the nuclear DNA content in two investigations with an interval of 21 months. The latter investigation was supplemented with chromosome analyses including quinacrine bonding. A modal chromosome number of 34 to 35 was found with a consistent loss of one chromosome Nos. 2, 3, 4, 5, 10, 13, 14, 15, 17, and 18, and no major structural changes. The corresponding calculated DNA content per nucleus correlated very well with the measured content, which was found to be 75% of the male diploid amount. The paper briefly discusses cell survival in extreme hypodiploidy and provides a comparison with cases from the literature in which banding analysis gives comparable information.
D001932:Brain Neoplasms / Q000032:analysis / Q000235:genetics*; D002648:Child; D002872:Chromosome Deletion; D004273:DNA, Neoplasm / Q000032:analysis*; D004171:Diploidy; D004806:Ependymoma / Q000032:analysis / Q000235:genetics*; D005434:Flow Cytometry; D006801:Humans; D007621:Karyotyping; D008297:Male
D004273:DNA, Neoplasm