The use of a tumor promoter as a single parameter approach for the detection of individuals genetically predisposed to colorectal cancer.
The effects of the tumor promoter, 12-O-tetradecanoyl phorbol-13-acetate (TPA), on the proliferation of cultured skin fibroblasts from individuals genetically prone to colorectal cancer have been investigated. Skin fibroblasts (SF) from both colorectal-prone and normal individuals displayed an unusual biphasic dose-response relationship, but the latter are considerably more sensitive to the toxic effects of TPA. The results indicate that measurement of cloning efficiency in the presence of a TPA probe, provides a reliable assay to further distinguish skin fibroblasts of colorectal-prone individuals from those of normal subjects in vitro.
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000473:pathology; D006801:Humans; D008297:Male; D010704:Phorbols / Q000494:pharmacology*; D012004:Rectal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate