[Oxidative-reductive activity of the mycelium as a criterion for assessing inoculate quality in gentamycin biosynthesis].
The development of the seed mycelium at various stages and temperatures was studied with the method of methylene blue decolorization characterizing the respiration level of the culture and its oxidation-reduction (dehydrogenase) activity. Relationship between the time of methylene blue decolorization by the seed mycelium, the quantity of the inoculum transferred from one stage to the other and the temperature of cultivation was shown. The seed mycelium decolorizing methylene blue within 2 minutes was most productive at the biosynthetic stage irrespective of the growth period, biomass volume, cultivation temperature and volume of the inoculum transferred from one stage to the other.
D003470:Culture Media; D004789:Enzyme Activation; D005839:Gentamicins / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D008751:Methylene Blue / Q000378:metabolism; D008847:Micromonospora / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D005839:Gentamicins; D010088:Oxidoreductases; D008751:Methylene Blue
Okislitel'no-vosstanovitel'naia aktivnost' mitsellia kak kriteriĭ otsenki kachestva inokuliata pri biosinteze gentamitsina.