A study of drug-induced kinetic perturbations in the marrow of a patient with neuroblastoma.
The kinetic perturbations induced by vincristine, cyclophosphamide, and adriamycin were studied in the bone marrow of a patient with neuroblastoma. Serial marrow samples were studied by dual parameter (Coulter volume and DNA content), flow cytometry, and radioautography, and findings were correlated with cytomorphologic changes. Multiparameter studies were useful in sorting out the various sequences of drug-induced kinetic changes. Distinctive effects of vincristine were observed at 2 and 24 hours after drug administration. An early drug-induced proliferative response was observed between 2 and 48 hours after initiation of therapy. Cell damage was apparent on day 4, with recovery in cell proliferative rate observed on days 6 through 9. The kinetics of perturbation may be useful in identifying temporal windows for optimum scheduling of chemotherapeutic agents in man.
D000293:Adolescent; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001345:Autoradiography; D001853:Bone Marrow / Q000032:analysis / Q000000981:diagnostic imaging / Q000187:drug effects*; D002452:Cell Count; D003520:Cyclophosphamide / Q000494:pharmacology; D004247:DNA / Q000032:analysis; D004317:Doxorubicin / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008297:Male; D009447:Neuroblastoma / Q000378:metabolism*; D011877:Radionuclide Imaging; D014750:Vincristine / Q000494:pharmacology
D000970:Antineoplastic Agents; D014750:Vincristine; D004317:Doxorubicin; D003520:Cyclophosphamide; D004247:DNA