LDH isoenzymes in cell cultures from transplantable hamster tumors with dissimilar histogenesis and differentiation.
LDH isoenzymes were assayed by acrylamide disc electrophoresis in the following material: (a) cells from 4 transplantable hamster tumors; (b) cells from the same tumors cultured for 0, 4, 12 or 24 h as well as for 3, 7, 12 or 26 days; (c) tumors obtained after re-inoculation of cells cultured for 26 days. During the first days of culture, the cells retained LDH patterns similar to those of tumors in vivo. Then a progressive increase in the M/H ratio was observed in all cultures. This was followed by a complete disappearance of LDH 1,2,3. In the tumors appearing after re-inoculation of cultured cells, re-emergence of the patterns seen in the original tumors was noted. The mechanism of the observed changes remains hypothetical although an adaptive re-arrangement of gene activity controlling the production of M and H subunits and/or in vitro selection of clones with higher M/H ratio is postulated.
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D006224:Cricetinae; D005260:Female; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008297:Male; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000201:enzymology* / Q000473:pathology; D014184:Transplantation, Homologous
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase