Synthesis of a 21-residue fragment of human proinsulin by the polyamide solid phase method.
The synthesis of human proinsulin fragment residues 40-60 by a solid phase method based on polydimethylacrylamide is described. Nineteen of the amino-acids were introduced as N alpha-(9-fluorenylmethoxycarbonyl) derivatives; functional side chains were protected as t-butyl ethers and esters. The yield of highly purified 21-residue peptide was 56.5%.
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D008722:Methods; D009757:Nylons; D010446:Peptide Fragments; D011384:Proinsulin / Q000138:chemical synthesis*
D007202:Indicators and Reagents; D009757:Nylons; D010446:Peptide Fragments; D011384:Proinsulin