Chemotaxis under agarose utilizing human serum depleted of C-5 derived peptides.
An immunoabsorbent column was made with antibody to trypsinized human C5. This column removed the chemotactic activity from zymosan-activated serum as well as from C5a des-arg-enriched fractions. Anti-trypsinized C5-absorbed human serum was substituted for unabsorbed human serum in the chemotaxis under agarose system. This resulted in significantly reduced random neutrophil migration with a negligible effect on C5a des-arg or FMLP-directed migration. The results indicate that much of the random migration observed in the chemotaxis under agarose system is due to C5-derived peptides present in normal human serum.
D000017:ABO Blood-Group System / Q000276:immunology; D000042:Absorption; D001769:Blood; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte; D003182:Complement C5 / Q000172:deficiency* / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D009239:N-Formylmethionine / Q000031:analogs & derivatives / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils; D009842:Oligopeptides / Q000494:pharmacology; D010455:Peptides / Q000172:deficiency; D011134:Polysaccharides; D012685:Sepharose; D014357:Trypsin / Q000494:pharmacology
D000017:ABO Blood-Group System; D003182:Complement C5; D009842:Oligopeptides; D010455:Peptides; D011134:Polysaccharides; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D012685:Sepharose; D014357:Trypsin