Clonal variation and functional correlation of organ-specific metastasis and an organ-specific metastasis-associated antigen.
Monoclonal antibodies were used to probe the cell surface of organ-selected metastatic variant cells. Previously we defined a liver metastasis-associated antigen (LMAA) by means of the reaction of a monoclonal antibody with a liver metastasis selected tumour variant cell line, MDCC-AL2. The monoclonal anti-LMAA antibodies specifically inhibit liver metastasis of AL2. There is a correlated, clonal variation in LMAA expression and liver metastasis in both the AL2 cell line and an overy-selected metastatic variant MDCC-AL3. The variation in liver metaatatic ability is thought to represent clonal progression of the tumour cell lines. The LMAA probably represents only one of the ways in which a tumour cell may give rise to a live metastasis. Two hypotheses are discussed utilizing the LMAA in a functional role in the specific trapping of metastatic tumour cells in the liver or the successful colonization of the liver by metastatic tumour cells.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis*; D002460:Cell Line; D002642:Chick Embryo; D002645:Chickens; D002999:Clone Cells / Q000276:immunology; D008113:Liver Neoplasms / Q000276:immunology* / Q000556:secondary; D008223:Lymphoma / Q000276:immunology*; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental / Q000276:immunology
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm