In-vitro binding of prostaglandin F-2 alpha to uterine luminal proteins of pregnant and non-pregnant ewes.
Pregnant and non-pregnant ewes were utilized to determine whether the presence of the embryo affected the binding of prostaglandin (PG) F-2 alpha to a uterine luminal protein. The uterine horn adjacent to the corpus luteum was flushed on Day 13 of gestation or the oestrous cycle. Flushings were incubated with [3H]PGF-2 alpha and subsequently eluted through a Sephadex column. Uterine luminal proteins of pregnant and non-pregnant ewes eluted with the void volume and failed to bind PGF-2 alpha.
D000818:Animals; D004971:Estrus; D005260:Female; D066298:In Vitro Techniques; D011247:Pregnancy; D011270:Pregnancy, Animal; D011460:Prostaglandins F / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*; D014599:Uterus / Q000378:metabolism*
D011460:Prostaglandins F; D011506:Proteins