Proton transfer in histidine hydrochloride induced by a dipolar aprotic solvent.
A 1H NMR study of histidine hydrochloride dissolved in water/dimethyl sulfoxide mixtures gives evidence that dimethyl sulfoxide is able to induce a proton transfer from the imidazolinium nitrogens (mainly N3) to the carboxylate group when its mole fraction becomes greater than 42%. The new tautomeric equilibrium AH2++- in equilibrium AH2+ is quantitatively studied as a function of the solvent composition. In pure dimethyl sulfoxide, this equilibrium is strongly biased toward the monocation AH2+, which represents 91% of the total histidine hydrochloride.
D004121:Dimethyl Sulfoxide; D006639:Histidine; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008433:Mathematics; D012997:Solvents
D012997:Solvents; D006639:Histidine; D004121:Dimethyl Sulfoxide