A new regulatory protein that affects the state of actin polymerization.
A new protein factor that regulates the state of actin polymerization was purified from porcine brain by DNase I-agarose chromatography. The purified protein factor consisted of a 1 : 1 complex of 88,000 dalton polypeptide and actin. When actin was polymerized by salt in the presence of the factor, the steady-state viscosity and the sedimentability were greatly reduced. The extent of the reductions was found to be greater in the presence of Ca2+ than in its absence.
D000199:Actins; D000818:Animals; D001923:Brain Chemistry; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D011108:Polymers; D011817:Rabbits; D013552:Swine; D014783:Viscosity
D000199:Actins; D009419:Nerve Tissue Proteins; D011108:Polymers