Fibroblast colonies in monolayer cultures of human bone marrow.
In a liquid culture of human bone marrow, the development of fibroblast colonies takes place on days 6 to 9. Twenty percent fetal calf serum is used as the stimulus fibroblast colony growth. Human bone marrow cells are plated as 2 x 10(5) cells in the culture. Normal human bone marrow yields 47 +/- 4 fibroblasts colonies per 2 x 10(5) cells plated. Bone marrow fibroblast cultures using agar or methylcellulose restrict colony formation. Marked colony suppression was observed in acute leukemia, and a discrete colony number was observed in hypoplastic anemia. This fibroblast culture method should be applied to a larger number of patients to determine whether it has a pathognomonic value and clinical significance.
D000208:Acute Disease; D000328:Adult; D000368:Aged; D000740:Anemia / Q000473:pathology; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D007938:Leukemia / Q000473:pathology; D008297:Male; D008875:Middle Aged