Accumulation of [3H]-ouabain in functionally different canine cardiac tissues: differential Rb+ uptake.
1 In order to understand tissue-specific differences in sensitivity to ouabain, the distribution of [3H]-ouabain was investigated in seven functionally different, canine cardiac tissues. 2 Specialized cardiac tissues (e.g., pacemaker or conducting tissues) accumulate much less ouabain than do the contractile tissues such as the left ventricle or the papillary muscle. 3 This study confirms a greater Rb+ uptake in the Purkinje fibre than in the contractile tissue. 4 The results disprove the hypothesis that a high accumulation of ouabain in the Purkinje fibre is responsible for the well-known sensitivity to ouabain in this tissue.
D000818:Animals; D004285:Dogs; D005260:Female; D006329:Heart Conduction System / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D010042:Ouabain / Q000378:metabolism*; D011690:Purkinje Fibers / Q000378:metabolism; D012413:Rubidium / Q000378:metabolism; D014316:Tritium
D014316:Tritium; D010042:Ouabain; D012413:Rubidium