A rapid and simple method for the determination of picogram levels of 3-methoxytyramine in brain tissue using liquid chromatography with electrochemical detection.
A rapid and simple technique using solvent extraction, ion-pairing extraction, and high pressure liquid chromatography with electrochemical detection has been developed for the determination of 3-methoxytyramine in striata of rats killed by microwave irradiation. The method is specific and reproducible (coefficient of variation among replications, +/- 4%); recovery of authentic 3-methoxytyramine added to the samples is 45-50%. 3-Methoxytyramine levels found with this technique in rat striata were 15 +/- 1.7 ng/g. The method has a sensitivity of about 0.2 pmol per brain sample. Monoamine oxidase inhibition with pargyline increased 3-methoxytyramine levels in rat striata, while catechol-O-methyltransferase inhibition with 3',4'-dihydroxy-2 methylpropiophenone completely depleted 3-methoxytyramine. The effects of nomifensine, quipazine, caroxazone, piribedil, and D-amphetamine were also examined. The 3-methoxytyramine concentrations in the brains of animals killed by decapitation or by microwave irradiation were compared.
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003342:Corpus Striatum / Q000032:analysis / Q000187:drug effects; D004298:Dopamine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D004563:Electrochemistry; D008297:Male; D008832:Microchemistry; D008872:Microwaves; D011180:Postmortem Changes; D051381:Rats
C001746:3-methoxytyramine; D004298:Dopamine