Structure determination of two oligomannoside-type glycopeptides obtained from bovine lactotransferrin, by 500 MHz 1H-NMR spectroscopy.
The elucidation of the structures of two carbohydrate units, N-glycosidically linked to an asparagine residue of bovine lactotransferrin, is described. These carbohydrate structures are of the oligomannoside type and contain eight or nine mannose residues, respectively. The potency of 500 MHz 1H-NMR spectroscopy in primary structure determination of two closely related carbohydrate chains present in a mixture is demonstrated. This implies that 500 MHz 1H-NMR spectroscopy can disclose microheterogeneity which is almost untraceable using other approaches.
D000818:Animals; D002236:Carbohydrate Conformation; D002417:Cattle; D007781:Lactoferrin; D007782:Lactoglobulins; D009682:Magnetic Resonance Spectroscopy
D007782:Lactoglobulins; D007781:Lactoferrin