High pressure liquid chromatographic-thermal energy determination of N-nitrosodiethanolamine in cosmetics.
Methods for the determination and confirmation of N-nitrosodiethanolamine (NDELA) in cosmetic products were developed. The NDELA fraction was isolated from a cosmetic product by a series of solvent extractions which were designed to concentrate the NDELA and remove ingredients deleterious to the analytical system. The isolated fraction was then analyzed for NDELA using a high pressure liquid chromatograph (HPLC) interfaced with a thermal energy analyzer (TEA). The compound was measured by comparison of detector response with those of known standards. NDELA was verified by gas chromatography-mass spectrometry of the silyl derivative after preliminary cleanup of the sample by gradient elution HPLC on a Partisil 10 PAC column. The limit of detection of NDELA by TEA is 2-3 ng, which corresponds to 20-30 ppb in the cosmetic product. Analysis of an emulsion cream and a hair grooming gel spiked at 3 and 4 ng concentration levels, respectively, yielded recoveries ranging from 71 to 103% (average 88%).
D002273:Carcinogens / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003358:Cosmetics / Q000032:analysis*; D004052:Diethylnitrosamine / Q000031:analogs & derivatives / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D009602:Nitrosamines / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013816:Thermodynamics
D002273:Carcinogens; D003358:Cosmetics; D009602:Nitrosamines; C014129:N-nitrosodiethanolamine; D004052:Diethylnitrosamine