[Stability of Penicillium vitale immobilized catalase in continuous decomposition of hydrogen peroxide].
The process of hydrogen peroxide continuous decomposition by the preparation of the fungus Penicillium vitale catalase immobilized by aminoorganosilica which were activated by glutaric aldehyde, cyanuric chloride or 2,4-toluylene diisocyanate. Catalase with an oxidized carbohydrate component was used as well. Such a modified enzyme was directly bound with the surface of aminocontaining silica and alumina. It is shown that in the process of H2O2 decomposition the preparations of immobilized catalase are inactivated. The decrease in its activity is described by a model which suggests that rates of hydrogen peroxide decomposition and enzyme inactivation are described by the first order equations. A method for calculation and prediction of mean time of continuous operation of columns with bound catalase and other immobilized enzymes is suggested in terms of the given model.
D002374:Catalase / Q000378:metabolism*; D004355:Drug Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D007700:Kinetics; D010407:Penicillium / Q000201:enzymology*
D004800:Enzymes, Immobilized; D006861:Hydrogen Peroxide; D002374:Catalase
Issledovanie stabil'nosti immobilizovannoĭ katalazy Penicillium vitale pri dlitel'nom razlozhenii perekisi vodoroda.