Genetic control of antisperm autoantibody response in vasectomized guinea pigs.
About 50% of vasectomized and none of sham-operated outbred Hartley GP develop autoantibodies to TSDA, surface antigens on sperm, spermatids, and residual bodies detectable by a sensitive quantitative cellular radioimmunobinding assay. A similar study of anti-TSDA antibody responses on different strains of GP demonstrates that Strain 13 GP are high responders and PLLhr guinea pigs are nonresponders, whereas the responses of Strain 2 and PLLlr GP are intermediate. Furthermore, 100% of vasectomized F1 GP between Strain 13 females and PLLhr males produce anti-TSDA antibody. Of the vasectomized back-cross GP between the F1 and PLLhr, 46% produce anti-TSDA antibody, 54% do not. These data provide strong evidence that autoimmune antibody response to sperm surface antigens in vasectomized GP is controlled by a single dominant autosomal or X-linked gene. Antisera from vasectomized Strain 13, F1, and back-cross GP are found to react with 3 major polypeptides (MW 63, 37, and 22 kd) on sperm surface that can be radioiodinated and extracted by NP-40.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics; D001323:Autoantibodies / Q000096:biosynthesis / Q000235:genetics*; D001324:Autoantigens / Q000235:genetics; D003433:Crosses, Genetic; D004822:Epididymis / Q000473:pathology; D006168:Guinea Pigs / Q000235:genetics*; D008297:Male; D013094:Spermatozoa / Q000276:immunology*; D013737:Testis / Q000276:immunology; D014659:Vasectomy
D000954:Antigens, Surface; D001323:Autoantibodies; D001324:Autoantigens