The effect of antiserum to S 100 protein on behavior and amount of S 100 in brain cells.
Since antiserum raised against the S 100 protein has an impairing effect on acquisition in behavioral tests, when interacting with S 100 on hippocampal cells, the effect of S 100 antiserum was studied in rats on the S 100 content of the hippocampus and thalamus, as well as on behavior. The operant reversal of handedness test and a light discrimination test were used. S 100 antiserum, 2 X 30 mu l, was injected intraventricularly before and during the sessions of two different learning tests. The S 100 protein was determined by quantitative immunoelectrophoresis. In the antiserum-injected animals, the levels of S 100 protein was increased by up to 30%, the incorporation values of 3H-valine increased in proteins of high molecular weight. Further acquisition was inhibited compared to controls, in which antiserum absorbed with pure S 100 protein was injected intraventricularly. The stimulation of S 100 synthesis, probably by the glia, may have occurred by a negative feedback effect, as has been observed in thymocytes.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D003216:Conditioning, Operant / Q000187:drug effects*; D004193:Discrimination Learning / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007839:Functional Laterality; D006624:Hippocampus / Q000032:analysis; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000276:immunology*; D010775:Photic Stimulation; D051381:Rats; D009418:S100 Proteins / Q000276:immunology*; D013788:Thalamus / Q000032:analysis
D009419:Nerve Tissue Proteins; D009418:S100 Proteins