Determination of thioproline in plasma using high performance liquid chromatography.
The determination of thioproline in plasma of cancer patients, using high performance liquid chromatography (HPLC) is reported. As column support, a silica-bonded cation exchanger was used. Detection was performed at 205 nm. The detection limit of the method was 5 X 10(-6) M and the linear dynamic range was over 500. No sample clean-up procedure was necessary other than deproteinization of the plasma. The method was applied to the measurement of plasma drug levels in 3 patients, part of a clinical trial testing the effectiveness of thioproline as an anti-cancer agent.
D000970:Antineoplastic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy; D013844:Thiazoles / Q000097:blood*; D053778:Thiazolidines
D000970:Antineoplastic Agents; D013844:Thiazoles; D053778:Thiazolidines; C003438:thiazolidine-4-carboxylic acid