Anaphase progression and furrow establishment in nocodazole-arrested PtK1 cells.
The relationship between progression through anaphase and furrow establishment was investigated in PtK1 cells using the anti-mitotic agent Nocodazole to arrest cells at different points in anaphase. The capacity of cells to furrow was compared to the kinetochore-kinetochore separation attained at the time of arrest. For the stages of anaphase examined, furrowing capacity increased directly with kinetochore-kinetochore separation until complete furrows were formed after kinetochore-kinetochore separations of 14 micron or more were reached. Furrow establishment thus occurs during a definite interval during anaphase in PtK1 cells. Results from electron microscopy of both Nocodazole-treated and control cells suggest that a population of astral microtubules may be important for furrow establishment.
D000705:Anaphase / Q000187:drug effects*; D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology*; D002219:Carbamates / Q000494:pharmacology*; D002460:Cell Line; D002875:Chromosomes / Q000648:ultrastructure; D007668:Kidney; D008394:Marsupialia; D008854:Microscopy, Electron; D015739:Nocodazole
D001562:Benzimidazoles; D002219:Carbamates; D015739:Nocodazole