Nocodazole inhibition of the vasopressin-induced water permeability increase in toad urinary bladder.
Nocodazole is a synthetic antitumor drug that binds rapidly to tubulin. When this drug is applied to toad bladder prior to vasopressin stimulation it inhibits the vasopressin response. A maximum inhibition (68%) is reached with a dose level of 10 micrograms/ml applied one-half hour prior to vasopressin stimulation (20 mU/ml). This compares with an inhibition of 50% seen with a 3-h exposure of the tissue to colchicine (0.1 mM) prior to stimulation with vasopressin. Application of nocodazole (1 microgram/ml) 3 min after hormonal stimulation shows no inhibition of the response at one-half hour past stimulation. These data support the view that microtubules are involved in the vasopressin-induced increase in water permeability in toad bladder and also indicate that this involvement is limited to the period prior to or directly after stimulation.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D002025:Bufonidae; D002219:Carbamates / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003078:Colchicine / Q000494:pharmacology; D015739:Nocodazole; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism*; D014667:Vasopressins / Q000037:antagonists & inhibitors*; D014867:Water / Q000378:metabolism*
D000970:Antineoplastic Agents; D001562:Benzimidazoles; D002219:Carbamates; D014867:Water; D014667:Vasopressins; D015739:Nocodazole; D003078:Colchicine