Two platelet aggregation inhibitors in tsetse (Glossina) saliva with studies of roles of thrombin and citrate in in vitro platelet aggregation.
Two inhibitors of platelet aggregation have been identified in saline extracts of Glossina morsitans (tsetse) salivary glands. A protein fraction (MW greater than 30 000) inhibited primary and secondary aggregation to ADP, secondary aggregation to adrenalin, and aggregation to collagen. It also caused disaggregation of platelets stimulated by ADP and adrenalin. These properties could be explained by ADP hydrolytic activity. A previously identified antithrombin fraction (MW 11 000-13 000) abolished thrombin-induced aggregation. It did not affect platelet aggregation to ADP, adrenalin or collagen, as compared to aggregation in citrate, when used as sole anticoagulant for platelet rich plasma or when added to citrated platelet rich plasma. These results fail to support hypotheses (i) that thrombin plays an important role in platelet aggregation by other agonists and (ii) that secondary platelet aggregation is an artefact induced by citrate. It is proposed that these inhibitors may be important in maintaining mouthpart and crop patency during feeding. Their discovery suggests that other arthropods may also have antiplatelet agents in their saliva which might be entomologically important, provide useful tools for platelet studies, and be of potential therapeutic interest.
D000818:Animals; D000991:Antithrombins / Q000494:pharmacology; D001779:Blood Coagulation Factors / Q000032:analysis* / Q000494:pharmacology; D002951:Citrates / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008970:Molecular Weight; D010974:Platelet Aggregation / Q000187:drug effects; D012463:Saliva / Q000032:analysis*; D012471:Salivary Proteins and Peptides / Q000032:analysis* / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology; D014370:Tsetse Flies / Q000032:analysis*
D000991:Antithrombins; D001779:Blood Coagulation Factors; D002951:Citrates; D012471:Salivary Proteins and Peptides; D013917:Thrombin