Correlation between map position and phenotype of CTI mutants in the c cistron of Rhizobium meliloti phage 16-3.
Nine temperature-sensitive clear mutations (Cti) in the C cistron (coding for the repressor protein) of Rhizobium meliloti temperate phage 16-3 were characterized according to the inductive temperature, the immunity of cells lysogenic for these mutant phages to superinfection by homoimmune weak virulent mutants, the phenotype of double-ti mutants and interallelic complementation. The results indicate that mutations of similar phenotypic expression are clustered on the genetic map. Furthermore, it seems probable that the C cistron of the original phage 16-3 is identical to that of the independently isolated phage strain 36.
D001435:Bacteriophages / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D005796:Genes; D005816:Genetic Complementation Test; D006358:Hot Temperature; D008242:Lysogeny; D009154:Mutation; D010641:Phenotype; D012231:Rhizobium / Q000235:genetics*