Effect of tritiated thymidine on the kinetics and viability of 9L cells in vitro.
The colony-forming efficiency of 9L rat gliosarcoma cells was unaffected by treatment with 0.1 muCi/ml of [3H]TdR. However, when cells were treated with 1 or 10 muCi/ml of [3H]Tdr, cell growth was reduced and cell survival decreased. When monolayer 9L cells were treated with 1 muCi/ml of [3H]TdR for up to 72 hr, approximately 5% survived, which is closely related to the percentage of non-cycling cells in this system. When cells were treated with 10 muCi/ml of [3H]TdR for 72 hr, less survival was observed. The additional cell kill observed may be induced by [3H]TdR released from doomed cells into petri dishes during the incubation period of the colony-formation assay.
D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003114:Colony-Forming Units Assay; D005910:Glioma / Q000473:pathology*; D007700:Kinetics; D009374:Neoplasms, Experimental / Q000473:pathology; D051381:Rats; D013936:Thymidine / Q000494:pharmacology*
D013936:Thymidine