Infrared spectroscopy and X-ray diffraction study of complexes of histones H3 and H4 in the condensed state.
Infrared spectroscopy, deuterium exchange kinetics and X-ray diffraction experiments on H3-H4 complexes in the condensed state are described. Both salt- and acid-extracted material are shown to contain alpha-helix in an amount comparable to that found under dilute solution conditions. Support for an anisotropic model of these complexes is provided.
D000818:Animals; D002417:Cattle; D006657:Histones / Q000302:isolation & purification; D007700:Kinetics; D046911:Macromolecular Substances; D011487:Protein Conformation; D013055:Spectrophotometry, Infrared; D013950:Thymus Gland; D014961:X-Ray Diffraction
D006657:Histones; D046911:Macromolecular Substances