Glyceroglucolipids of the mucous barrier of dog stomach.
Distribution of glyceroglucolipids in the mucous barrier of dog stomach fundus, body and antrum was investigated. Surface mucus lining and preformed intracellular mucus were obtained by instillation of the ligated stomach compartments with 2 M NaCl. Lipids were extracted from the dialyzed and lyophilized samples, and the glycolipids were separated into neutral and acidic fractions. The glyceroglucolipids contained in each fraction were purified into individual components by thin-layer chromatography and were quantified. The content of glyceroglucolipids (mumol glucose/g protein) in the antral portion of the stomach was 2.1 times greater than that of the fundus and 3.4 times greater than that of the body. All three areas of the stomach contained neutral and sulfated glyceroglucolipids. However, the level of sulfated glycerolglucolipids (mumol glucose/g protein) was three times higher in the antrum as compared to the fundus and four times higher as compared to the body. The neutral and sulfated glyceroglucolipids were present in a molar ratio of 1.0 : 0.7 in the fundus, 1.0 : 1.1 in the body and 1.0 : 1.5 in the antrum.
D000818:Animals; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004285:Dogs; D005753:Gastric Mucosa / Q000032:analysis*; D005989:Glycerides / Q000032:analysis; D006017:Glycolipids / Q000032:analysis*; D008055:Lipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012965:Sodium Chloride
D005989:Glycerides; D006017:Glycolipids; D008055:Lipids; D011506:Proteins; C016476:glyceroglucolipids; D012965:Sodium Chloride