Intracerebral grafting of dissociated CNS tissue suspensions: a new approach for neuronal transplantation to deep brain sites.
Suspensions of central nervous tissue, prepared by dissociation of selected embryonic brain regions, may be viably transplanted by intraparenchymal injection into a variety of sites in the depth of adult rat host brains. Such grafted neurons can mediate considerable reinnervation of a previously denervated brain region, and they can replace neurons intrinsic to a particular target, such as the caudate-putamen, previously damaged by the neurotoxin kainic acid. The present technique should open entirely new possibilities for experiments on neuronal reconstruction following brain lesions.
D000818:Animals; D001921:Brain / Q000196:embryology; D003342:Corpus Striatum / Q000637:transplantation; D004298:Dopamine / Q000032:analysis; D005260:Female; D007276:Injections, Intraventricular; D007608:Kainic Acid / Q000494:pharmacology; D008636:Mesencephalon / Q000637:transplantation; D009474:Neurons / Q000637:transplantation*; D011247:Pregnancy; D051381:Rats; D013378:Substantia Nigra / Q000637:transplantation; D014184:Transplantation, Homologous
D007608:Kainic Acid; D004298:Dopamine