The onset of polymorphonuclear leucocyte membrane-stimulated metabolic activity.
The time course of polymorphonuclear leucocyte oxidative metabolism following membrane stimulation by four different agents was examined using the techniques of luminol- and lucigenin-dependent chemiluminescence. After addition of opsonized zymosan, phorbol myristate acetate or digitonin to polymorphonuclear leucocytes there was a lag period of between 35 and 55 sec before the onset of chemiluminiscence. In contrast, after addition of the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP), the lag period before chemiluminescence was less than 19 sec. Luminol-dependent chemiluminescence was reduced by superoxide dismutase and almost abolished by sodium azide. The inhibitory effect of the latter was less marked when using FMLP. Lucigenin-dependent chemiluminescence was inhibited by superoxide dismutase and enhanced by sodium azide. Cytochalasin B reduced zymosan and digitonin stimulated chemiluminescence but increased FMLP stimulated chemiluminescence. The results of the onset of polymorphonuclear leucocyte metabolic activity using other techniques.
D000963:Antimetabolites / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003571:Cytochalasin B / Q000494:pharmacology; D004072:Digitonin / Q000494:pharmacology; D006801:Humans; D008163:Luminescent Measurements; D009239:N-Formylmethionine / Q000031:analogs & derivatives / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D015054:Zymosan / Q000494:pharmacology
D000963:Antimetabolites; D009842:Oligopeptides; D003571:Cytochalasin B; D009239:N-Formylmethionine; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015054:Zymosan; D004072:Digitonin; D013755:Tetradecanoylphorbol Acetate