High-performance liquid chromatographic determination of verapamil in plasma by fluorescence detection.
A rapid and highly sensitive method is reported for the quantitative determination of verapamil in plasma. Verapamil and its internal standard are extracted from alkalinized plasma with heptane and then back-extracted into dilute sulfuric acid. An aliquot is injected directly into a high-performance liquid chromatograph, separated by reversed-phase chromatography, and quantified by a fluorescence detector. The procedure is suitable for the routine determination of verapamil in plasma in concentrations as low as 1 ng/ml.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D006207:Half-Life; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors; D014700:Verapamil / Q000097:blood*
D014700:Verapamil