A quantitative assessment of dystrophic mouse (129 ReJ dy/dy) myogenesis in vitro.
A quantitative assessment was made of the myogenic capability in vitro of muscle cells from dystrophic (129 Rej dy/dy) and normal mice from birth to 5 months old. Seeding efficiency was increased in dystrophic cells from neonatal and 1-week-old mice compared to age-matched controls. The extent of myogenesis in cultures from neonatal and 1-week-old dystrophic mice did not differ from controls. Muscle colony formation in cultures established from 5-month-old dystrophic mice was reduced by 80% compared with normal cultures. Normal and dystrophic cultures established from 5-month-old mice contained equal numbers of fibroblast colonies. The results suggest that decreased myogenesis in cultures from 5-month-old dystrophic mice is due to a relative absence of myogenic cells rather than a numerical dilution of the cultures by fibroblasts. This may be due to population of nonviable satellite cells or to necrosis of dystrophic myotubes in vitro.
D000375:Aging; D000818:Animals; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D005121:Extremities / Q000033:anatomy & histology; D005347:Fibroblasts / Q000503:physiopathology; D006128:Growth; D006412:Hematopoietic Stem Cells / Q000503:physiopathology; D051379:Mice; D009132:Muscles / Q000473:pathology / Q000502:physiology*; D009137:Muscular Dystrophy, Animal / Q000503:physiopathology*; D012038:Regeneration