[Genetic control of toxin formation by Vibrio cholerae. I. Mobilization of the tox gene of Vibrio cholerae by plasmid RP::Mu ctsS62].
The possibility of the mobilization of V. cholerae genes responsible for toxin formation (tox gene) and serological specificity (oag-I gene, serotype Inaba) by the RP4::Mu cts62 plasmid was established. The cotransfer of tox and oag-I genes with his and ilv genes respectively by means of the RP4::Mu cts62 plasmid was revealed. The frequency of the cotransfer of tox his and oag-I ilv observed in our experiments corresponded to the known frequency of the cotransfer of these genes observed in crosses with V. cholerae P+-strains. In recombination clones selected according to chromosomal amino acid markers (His+, Ilv+), both carrying and not carrying tox and oag-I genes, no markers of the RP4 plasmid and phage Mu cts62 were detected. The only exception was one His+-recombinant clone. It possessed thermosensitivity (the marker of phage Mu cts62), not linked, however, with sensitivity to phage thermoinduction. Similar clones were obtained when the plasmid was eliminated from V. cholerae strains containing the RP4::Mu cts62 plasmid and having the phenotype corresponding to this plasmid.
D002772:Cholera Toxin / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D002876:Chromosomes, Bacterial; D002999:Clone Cells; D004589:Electrophoresis, Disc; D005796:Genes; D010957:Plasmids; D011995:Recombination, Genetic; D014734:Vibrio cholerae / Q000235:genetics* / Q000378:metabolism
D002772:Cholera Toxin
Geneticheskiĭ kontrol' toksinoobrazovaniia u kholernykh vibrinov. Soobshchenie I. Mobilizatsiia gena tox Vibrio cholerae plazmidoĭ RP::Mu ctsS62.