Cystathionine gamma-lyase activity in the cephamycin C producer Streptomyces lactamdurans.
Extracts of the cephamycin C producer S. lactamdurans were found to possess cystathionine gamma-lyase activity (E.C. 4.4.1.1). This represents the first demonstration of this enzyme of the reverse transsulfuration pathway in a prokaryotic organism. A likely involvement of reverse transsulfuration in antibiotic synthesis is indicated by the fact that propargylglycine, a mechanism-based inhibitor of the gamma-lyase, is a strong inhibitor of cephamycin C production.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D019757:Carbon-Oxygen Lyases; D002511:Cephalosporins / Q000096:biosynthesis*; D002513:Cephamycins / Q000096:biosynthesis*; D005285:Fermentation; D006714:Homoserine / Q000031:analogs & derivatives / Q000378:metabolism; D008190:Lyases / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology / Q000378:metabolism*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D002513:Cephamycins; D006714:Homoserine; C022696:O-succinylhomoserine (thiol)-lyase; D008190:Lyases; D019757:Carbon-Oxygen Lyases; C024925:cephamycin C