[Physiological importance of microvilli-bound leucine arylamidase in the final digestion of proteins. 1. Purification and isolation of intestinal leucine arylamidase and aminotripeptidase of rats].
The membrane-bound leucine arylamidase of the microvilli of the rat small intestine was solubilized by Triton X-100 and purified by gel and ion-exchange chromatography. As compared to the mucosa homogenate, the purification factor was 135. The leucine arylamidase and aminotripeptidase of the microvilli cannot be separated by chromatography. The cytosomal portion of the aminopeptidase is devoid of leucine arylamidase activity.
D000626:Aminopeptidases / Q000302:isolation & purification*; D000818:Animals; D002850:Chromatography, Gel; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D007413:Intestinal Mucosa / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology*; D007931:Leucyl Aminopeptidase / Q000302:isolation & purification*; D008566:Membranes / Q000201:enzymology; D008871:Microvilli / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000201:enzymology
D000626:Aminopeptidases; D007931:Leucyl Aminopeptidase; C026045:tripeptide aminopeptidase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
Untersunchungen zur physiologischen Bedeutung der bürstensaumgebundenen Leucinarylamidase. für die Endverdauung von Proteinen. 1. Mitt. Reinigung und Isolierung der intestinalen Leucinarylamidase und Aminotripeptidase der Ratte.