Quantitative analysis of cephamycin C in fermentation broths by means of thin-layer spectrodensitometry.
A method has been developed that consists of one-dimensional thin-layer chromatography of the samples on pre-coated silica gel 60F254 plates followed by an in situ spectrophotometric evaluation of the chromatograms at 273 nm. Statistical evaluations showed a linear calibration (r2 greater than 0.999) for the range of 0.2-20 microgram/spot. The relative standard deviations for broth samples containing 1.505 and 12.47 microgram Cephamycin C per spot were 3.61 and 1.07%, respectively. The recovery was virtually quantitative: 97.6%. The correlation with a liquid chromatographic method was quite acceptable with r2 = 0.99124 and b = 0.998 (slope).
D002440:Cefoxitin / Q000096:biosynthesis; D002511:Cephalosporins / Q000032:analysis*; D002513:Cephamycins / Q000032:analysis*; D002855:Chromatography, Thin Layer; D005285:Fermentation; D011786:Quality Control; D013678:Technology, Pharmaceutical
D002511:Cephalosporins; D002513:Cephamycins; D002440:Cefoxitin; C024925:cephamycin C