Erythrocyte membrane abnormalities in experimental biliary obstruction: comparative studies on erythrocyte membranes from rats with intra- and extrahepatic cholestasis.
The red blood cells from rats with intra- and extrahepatic cholestasis obtained by feeding of alpha-naphthylisothiocyanate (ANIT) and ligation of bile duct, respectively, were analysed from biochemical as well as ultrastructural point of view. A significant increase of cholesterol content was observed in the red cell membrane from bile duct-ligated rat. An increment of absolute lecithin content without accompanied increase in phospholipid content was observed in both groups. The fatty acyl chain composition of lecithin remained unchanged except a small increase in stearic acid (13.0% leads to 18.1%) in the bile duct-ligated rat. Despite a small change in the lipid composition, scanning electron microscopic observation revealed a profound alteration in red cell ultrastructure; most of cells were in spur type in ANIT-fed group while variations in red cell shape were observed in bile duct-ligated group.
D000818:Animals; D001647:Bile Acids and Salts / Q000097:blood; D001663:Bilirubin / Q000097:blood; D002779:Cholestasis / Q000097:blood* / Q000473:pathology; D004910:Erythrocyte Membrane / Q000032:analysis* / Q000648:ultrastructure; D004912:Erythrocytes / Q000032:analysis*; D008297:Male; D008563:Membrane Lipids / Q000032:analysis; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D011919:Rats, Inbred Strains
D001647:Bile Acids and Salts; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010743:Phospholipids; D001663:Bilirubin