Detection of AB antigens on human endothelial cells derived from umbilical cord by measurement of 14C adenine uptake.
We have used the uptake of (14C) adenine by human endothelial cells derived from umbilical cord to determine the presence of antigens of the ABO system on endothelial cells in culture. The results indicate that the same ABO type was detectable on endothelial cells by inhibition of the (14C) adenine uptake and on erythrocytes of the cord blood vessel by classical hemagglutination.
D000017:ABO Blood-Group System; D000225:Adenine / Q000378:metabolism*; D002250:Carbon Radioisotopes; D004727:Endothelium / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007700:Kinetics; D014470:Umbilical Cord / Q000276:immunology*
D000017:ABO Blood-Group System; D002250:Carbon Radioisotopes; D007106:Immune Sera; D000225:Adenine