[Study of endometrial hyperplasia employing the method of micro-electrophoresis of cultured cells of hyperplastic endometrium (author's transl)].
Endometrial hyperplasia was studied from the point of view of cell surface charge employing micro electrophoresis. Surface change of cells corresponding to the cyclic changes of cell multiplication as well as estrogen and/or progesterone effects on the cellular surface charges on each phase of the cell cycle. The results as follows: 1. Electrophoretic mobility was highest in mitotic phase. This result was indicative of what the cell-surface charge was closely related to cell multiplication. 2. Estrogen clearly elevated electrophoretic mobility of cells in synthetic phase. Progesterone lowered electrophoretic mobility of cells in G1 phase. These results indicate that multiplication was forced in synthetic phase by estrogen and differentiation in G1 phase by progesterone. 3. Effects of estrogen and progesterone to cultured pulmonary cancer cells were studied as control to endometrial ones which might be target to these hormones. No definite results as previously shown on endometrial cells were obtained on the pulmonary ones. As a result, it is apparent that endometrium is a target to these hormones. Determination of the cellular surface charge by cell micro electrophoresis indicated that estrogen might play an important role in triggering abnormal proliferations of the endometrium.
D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D004586:Electrophoresis; D004714:Endometrial Hyperplasia / Q000473:pathology*; D004717:Endometrium / Q000473:pathology*; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D006801:Humans; D011374:Progesterone / Q000494:pharmacology
D004967:Estrogens; D011374:Progesterone