Analysis of genome composition and reactogenicity of recombinants of cold-adapted and virulent virus strains.
Temperature-sensitive (ts) mutations occurring in three genes, 1, 2, and 7, and 1, 5 and 7 were found in two cold-adapted (ts) attenuated influenza virus strains A/Leningrad/9/37/46 (H0N1) and A/Leningrad/134/17/57 (H2N2) respectively. The recombinants, obtained by crossing these cold-adapted strains with virulent influenza virus strains, had different genome structures and inherited from one to six genes from the cold-adapted parents. Tests of reactogenicity of recombinants in volunteers showed all the recombinants to be non-reactogenic irrespective of the number of genes inherited from the cold-adapted parent.
D000328:Adult; D000914:Antibodies, Viral / Q000096:biosynthesis*; D003080:Cold Temperature; D005814:Genes, Viral; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D009154:Mutation; D011995:Recombination, Genetic; D014611:Vaccination; D014764:Viral Proteins / Q000032:analysis
D000914:Antibodies, Viral; D014764:Viral Proteins