[Gas chromatographic determination of phenytoin, phenobarbital and primidone: flash-methylation after direct addition of trimethyl-phenyl-ammonium hydroxide to the ethyl acetate extract (author's transl)].
25 microliters plasma, 25 microliters internal standard and 5 microliters sodium dihydrogenphosphate solution are extracted with 100 microliters ethyl acetate. 40 microliters of the extract are mixed with 10 microliters trimethyl-phenyl-ammoniumhydroxide (0.1 mol/l methanol). 3 microliters of this solution are injected for gas chromatography using a nitrogen-specific detector. Trimethyl-phenyl-ammoniumacetate appears to be a satisfactory methylating agent for these drugs in the injection port of the gas chromatograph.
D000814:Aniline Compounds; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D008745:Methylation; D010634:Phenobarbital / Q000097:blood*; D010672:Phenytoin / Q000097:blood*; D011324:Primidone / Q000097:blood*; D000644:Quaternary Ammonium Compounds
D000814:Aniline Compounds; D000644:Quaternary Ammonium Compounds; D011324:Primidone; C009571:phenyltrimethylammonium; D010672:Phenytoin; D010634:Phenobarbital
Gaschromatographische Bestimmung von Phenytoin, Phenobarbital und Primidon: Methylierung im Einspritzblock nach direktem Zusatz von Trimethyl-phenyl-ammonium-hydroxid zum Ethylacetat-Extrakt.