Quantitative estimation of the extent of alkylation of DNA following treatment of mammalian cells with non-radioactive alkylating agents.
Alkaline sucrose sedimentation has been used to quantitate phosphotriester formation following treatment of human cells with the monofunctional alkylating agents methyl and ethyl methanesulfonate. These persistent alkaline-labile lesions are not repaired during short-term culture conditions and thus serve as a useful and precise index of the total alkylation of the DNA. Estimates of alkylation by this procedure compare favorably with direct estimates by use of labeled alkylating agents.
D000477:Alkylating Agents; D002499:Centrifugation, Density Gradient / Q000379:methods; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010710:Phosphates; D013997:Time Factors
D000477:Alkylating Agents; D010710:Phosphates; D004247:DNA