Evaluation of chronic granulomatous disease by a chemiluminescence assay of microliter quantities of whole blood.
In our modification of a chemiluminescence assay to detect chronic granulomatous disease of childhood, whole blood is used and dark-adaptation is not required. This improvement was made possible by the use of luminol as an amplification device and of phorbol myristate acetate as a stimulant. Using this simplified assay, we can assess neutrophil function with 25-100 microL of whole blood, permitting simple evaluation even in very young pediatric patients.
D002648:Child; D006105:Granulomatous Disease, Chronic / Q000175:diagnosis*; D006801:Humans; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D008832:Microchemistry / Q000379:methods; D009504:Neutrophils / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D008165:Luminol; D015054:Zymosan; D013755:Tetradecanoylphorbol Acetate