Inhibitory action of intravenously administered ammonium acetate on the motility of the rumen in sheep.
The effect of 60 minutes' intravenous infusions, before morning feeding, of ammonium acetate (18.6 micromole/min/kg of body weight) and ammonium acetate with propranolol (11 microgram/min/kg of body weight) on the ruminal motility of sheep was examined. Ammonia has a adrenaline-like action therefore propranolol, a beta-receptor blocking agent, was administered in order to eliminate the possible effect of adrenaline on ruminal motility. The contractions of the dorsal sack of the rumen were registered by means of the balloon method, with the gauge inserted through the rumen fistula. The infusion of ammonium acetate caused an increase of the ammonia concentration in the blood to 0.6 mmol/1 at the end of 60 minutes' infusion. Already during the first 5 minutes of the intravenous infusion of ammonium acetate there was a decreased frequency of ruminal contractions, which was observed throughout the infusion. After the infusion there was a radical decrease of the concentration of ammonia in the blood, and at the same time an increase in the frequency of rumen contraction was observed. Blocking of the beta-adrenergic receptors by propranolol did not eliminate the inhibiting action of ammonium ion on rumen motility. The infusion of the ammonium acetate caused an increase of adrenaline and glucose concentration. This response was eliminated by propranolol in the case of adrenaline but not glucose. It is assumed that the action of ammonium ion on the rumen motility is derived primarily by the central nervous system.
D000085:Acetates; D000641:Ammonia / Q000037:antagonists & inhibitors / Q000097:blood; D000818:Animals; D005769:Gastrointestinal Motility / Q000187:drug effects*; D007263:Infusions, Parenteral; D008297:Male; D011433:Propranolol / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000008:administration & dosage*; D012417:Rumen / Q000187:drug effects*; D012756:Sheep / Q000502:physiology*
D000085:Acetates; D000644:Quaternary Ammonium Compounds; D000641:Ammonia; D011433:Propranolol; C018824:ammonium acetate