Increased content of microbicidal cationic peptides in rabbit alveolar macrophages elicited by complete Freund adjuvant.
We measured the microbicidal peptides MCP-1 and MCP-2 in rabbit alveolar macrophages (AM), comparing rabbits pretreated with complete Freund adjuvant with untreated control animals. Levels of MCP-1 increased from 4.7 +/- 0.6 microgram/10(7) resident AM to 13.2 +/- 0.1 microgram/10(7) complete Freund adjuvant-elicited AM. MCP-2 levels rose from 1.8 +/- 0.1 microgram/10(7) resident AM to 7.3 +/- 0.4 microgram/10(7) complete Freund adjuvant-elicited AM. The activities of five lysosomal hydrolases (beta-D-glucuronidase, beta-D-galactosidase, acid phosphatase, N-acetyl-beta-D-galactosaminidase, and N-acetyl-beta-D-glucosaminidase) were 44 to 96% higher in complete Freund adjuvant-elicited AM, and lysozyme activity was three- to fourfold higher. As MCP-1 and MCP-2 are major constituents of rabbit AM and exhibit potent antibacterial and antifungal properties, they may contribute to the expression of microbicidal activity in both resident and activated states.
D000818:Animals; D000890:Anti-Infective Agents / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides; D005620:Freund's Adjuvant / Q000494:pharmacology*; D006867:Hydrolases / Q000378:metabolism; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism*; D009113:Muramidase / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D011650:Pulmonary Alveoli; D011817:Rabbits
D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D011506:Proteins; D005620:Freund's Adjuvant; D006867:Hydrolases; D009113:Muramidase