Serological identification of viral antigens after electrophoretic transfer.
The method described permits detection of specific antibodies to antigenic material, the protein components of which can first be separated by electrophoresis and then transferred to a supporting medium where their reactivity with antibody may be demonstrated using peroxidase-labelled anti-Ig. Results are described for measles virus and measles-like virus isolated from subacute sclerosing panencephalitis (SSPE) brain. Dilutions, incubation time and conditions, detection with peroxidase-labelled immunoglobulins and selection of substrate are described and discussed.
D000936:Antigen-Antibody Complex; D000956:Antigens, Viral / Q000032:analysis*; D001921:Brain / Q000276:immunology; D003101:Collodion; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005374:Filtration; D006801:Humans; D008459:Measles virus / Q000276:immunology*; D010209:Paper; D013344:Subacute Sclerosing Panencephalitis / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D003101:Collodion