Resonance Raman spectra of three-iron centers in ferredoxins from Desulfovibrio gigas.
The resonance Raman spectra of ferredoxins (Fd) I and II from Desulfovibrio gigas are reported using 4579 A Ar+ laser excitation. The (3Fe-3S) center in Fd II has a characteristic resonance Raman spectrum, readily distinguishable from those of (2Fe-2S) or (4Fe-4S) clusters. Reduction of Fd II produces a marked alteration in the resonance Raman spectrum. Fd I is shown to contain both (3Fe-3S) and (4Fe-4S) Fd-type clusters. The results illustrate the potential of resonance Raman spectroscopy in Fe-S cluster identification, even in cases where more than one cluster type is present.
D001665:Binding Sites; D003901:Desulfovibrio / Q000032:analysis*; D005288:Ferredoxins; D007501:Iron / Q000032:analysis*; D011485:Protein Binding; D011487:Protein Conformation; D013059:Spectrum Analysis, Raman
D005288:Ferredoxins; D007501:Iron