Either high-mannose-type or hybrid-type oligosaccharide is linked to the same asparagine residue in ovalbumin.
After pepsin digestion, all of the carbohydrates in ovalbumin were recovered in two glycopeptides, Glu-Glu-Lys-Tyr-Asn(CHO)-Leu-Thr-Ser-Val and Glu-Gln-Lys-Tyr-Asn(CHO)-Leu-Thr-Ser-Val. Almond glycopeptidase released quantitatively oligosaccharides from the glycopeptides. The products from both glycopeptides contained both the high-mannose-type oligosaccharides and the hybrid-type oligosaccharides in the same ratio. Thus, either the high-mannose-type or the hybrid-type oligosaccharide is attached to the unique asparagine residue in the ovalbumin molecule.
D000581:Amidohydrolases; D000818:Animals; D001216:Asparagine; D002645:Chickens; D006020:Glycopeptides; D008358:Mannose / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis*; D010047:Ovalbumin; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D010944:Plants / Q000201:enzymology
D006020:Glycopeptides; D009844:Oligosaccharides; D001216:Asparagine; D010047:Ovalbumin; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D008358:Mannose