A direct plaque assay for Mount Elgon bat virus.
Direct plaque formation by Mount Elgon bat virus was obtained using Vero cells under overlays of carboxymethyl cellulose or agar. The plaques were small but easily countable when the monolayer was stained with crystal violet. The direct assay was simple and convenient and gave infectivity titres slightly higher than those obtained by the indirect method.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D005840:Gentian Violet; D012209:Rhabdoviridae / Q000254:growth & development*; D010948:Viral Plaque Assay / Q000379:methods*
D005840:Gentian Violet