Susceptibility of protein synthesis to neomycin in neomycin-producing Streptomyces fradiae.
A cell-free protein-synthesizing system from Streptomyces fradiae was developed by preparing ribosomes and an S-150 fraction with precautions to prevent protease action. Using this system, the ribosomes of this organism were shown to be susceptible to its own product, neomycin.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D009355:Neomycin / Q000096:biosynthesis* / Q000494:pharmacology; D010649:Phenylalanine / Q000096:biosynthesis; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D013302:Streptomyces / Q000187:drug effects / Q000378:metabolism*
D001426:Bacterial Proteins; D010649:Phenylalanine; D009355:Neomycin