Cortisol resistant RPMI-1788 lymphocytes become sensitive to cortisol subsequent to a 24-h incubation period in medium containing purified human transcortin.
RPMI-1788 lymphocytes (a human cell line) are resistant to cortisol in vitro. Prior incubation for a minimum of 24 h in a medium which contains purified human transcortin at a concentration of 50 micrograms/ml renders these cells sensitive to the inhibitory action of cortisol as regards the synthesis of DNA. Only the transcortin-exposed cells contain a cortisol binding species whose sedimentation behavior in a sucrose gradient is identical to that of transcortin.
D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004351:Drug Resistance; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D011965:Receptors, Glucocorticoid / Q000378:metabolism; D014156:Transcortin / Q000494:pharmacology*
D011965:Receptors, Glucocorticoid; D004247:DNA; D014156:Transcortin; D006854:Hydrocortisone