[Immobilization of Penicillium vitale glucose oxidase by aminoethyl cellulose and properties of immobilized enzyme].
Penicillium vitale glucose oxidase modified by oxidation of the carbohydrate component is in a covalent combination with aminoethyl cellulose (AE-cellulose). With the optimal enzyme: carrier ratio the manifested activity of glucose oxidase in the preparation is 4.3 +/- 0.8% of the attached enzyme activity. It is established that thermostability, stability to an inactivating effect of a labilizing fraction and pH-stability with pH alkaline values in the immobilized glucose oxidase are higher than in the soluble enzyme. It is shown that the process of thermal and alkaline inactivation occurs through two stages differing in the rate. The properties of P. vitale glucose oxidase immobilized by the same method by different carriers, aminosilochrome and AE-cellulose are compared.
D002482:Cellulose / Q000031:analogs & derivatives; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism*; D007700:Kinetics; D008722:Methods; D010407:Penicillium / Q000201:enzymology*
D004800:Enzymes, Immobilized; D002482:Cellulose; C010021:aminoethyl cellulose; D005949:Glucose Oxidase
Immobilizatsiia gliukozooksidazy Penicillium vitale na amonoetiltselliuloze i svoĭstva immobilizovannogo fermenta.