[ATP dependent calcium uptake activity in plasma membrane fraction of intestinal smooth muscle (author's transl)].
Ca2+ binding and ATP dependent Ca2+ uptake by plasma membrane fraction were studied and the effect of treating plasma membrane fraction with trypsin, phospholipase c and neuraminidase on both activities was observed. 1. Plasma membrane fraction possessed the ability to bind Ca2+. The Scatchard plot of Ca2+ binding showed that plasma membrane had at least two types of Ca2+ binding sites of high and low affinity. 2. The amount of Ca2+ uptake by plasma membrane fraction was 18.51 +/- 1.31 nmoles Ca2+/mg protein/30 min at 10(-5)M Ca2+. The Ca2+ concentration for half maximal activation in Ca2+ uptake was 3.7 x 10(-7)M. 3. The ATP dependent Ca2+ uptake by the microsome isolated from guinea pig intestine was clearly stimulated by 4 mM K2-oxalate whereas that of plasma membrane fraction was not. 4. Trypsin and phospholipase c treatment led to a 40--88% reduction in the ATP dependent Ca2+ uptake. The Ca2+ concentration for half maximal activation Ca2+ uptake shifted to a high concentration. On the other hand, neuraminidase treatment resulted in a 43--93% increase in ATP dependent Ca2+ uptake. But, the Ca2+ concentration for half maximal activation in Ca2+ uptake was not shifted by neuraminidase treatment. The results show that plasma membrane plays an important role in regulating the cytoplasmic Ca2+ concentration. The results also suggest that a glycoprotein linked with sialic acid is involved in the flux of Ca2+ across the plasma membrane.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism*
D000255:Adenosine Triphosphate; D002118:Calcium