Isolation of glycogen from buffalo liver.
The glycogen has been isolated from buffalo (Bos Bubalus bubalis) liver, purified several-fold, and characterized to compare with rabbit and oyster glycogen. Once-purified buffalo glycogen has been found to contain 1.3% protein, 0.16% nitrogen, 0.69% phosphorous, no lipids, and nucleic acids sufficient to cause absorption at 260 mu. The buffalo glycogen may be used as a potential substitute for rabbit and oyster glycogen after two-or three- purifications and a treatment with DEAE-cellulose.
D000818:Animals; D002020:Buffaloes / Q000378:metabolism*; D008112:Liver Glycogen / Q000032:analysis / Q000302:isolation & purification*; D010125:Ostreidae; D010758:Phosphorus / Q000032:analysis; D011506:Proteins / Q000032:analysis; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet
D008112:Liver Glycogen; D011506:Proteins; D010758:Phosphorus