Freeze-fracture analysis of the effects of age and 2,4-dinitrophenol on the morphology of flight muscle mitochondria of Musca domestica L.
Morphometric comparison of freeze-fractured mitochondria in flight muscles of adult (37-day-old) and old (68-day-old) houseflies revealed a 28% decrease of cristae in the old flies. The major membrane change with age was an increase in the 90-120-A particles in the inner membrane external face concomitant with a loss of particle clusters associated with the openings of the cristae on to the inner membrane. In vitro treatment of flight muscle with 2,4-dinitrophenol, and uncoupler of mitochondrial respiration, did not produce this change but resulted in the formation of smooth particle-free vesicular swellings in the mitochondria. Such swelling were infrequent in the old muscle. The cause for the aging change is not clear, but a reduction in the ability of the intramembranous particles to aggregate, either through modification of altered synthesis, is indicated.
D019297:2,4-Dinitrophenol; D000375:Aging; D000818:Animals; D004140:Dinitrophenols / Q000494:pharmacology*; D005614:Freeze Fracturing; D006793:Houseflies / Q000648:ultrastructure*; D007425:Intracellular Membranes / Q000648:ultrastructure; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000648:ultrastructure*; D008933:Mitochondrial Swelling / Q000187:drug effects; D014475:Uncoupling Agents / Q000494:pharmacology
D004140:Dinitrophenols; D014475:Uncoupling Agents; D019297:2,4-Dinitrophenol