Specific assay for radiolabelled digoxin and its known apolar metabolites in biological fluids. I.
A specific assay method for radiolabelled digoxin and its known apolar metabolites in plasma, urine and saliva was developed. The assay permits the delineation of the pharmacokinetics of digoxin and its metabolites after single-dose administration of the drug to humans. Column chromatographic and solvent extraction procedures were used for the separation of apolar and polar compounds. Thin-layer chromatography was applied for the individual and specific assessment of digoxin and its apolar metabolites. Apolar and polar standards were used for quantitative assessments of all the procedures used. Accuracy and precision of the assay developed were evaluated in plasma, urine and saliva using biological samples spiked with known amounts of standards and by measuring replicates of biological samples obtained from pharmacokinetic studies wit digoxin administration to humans.
D002855:Chromatography, Thin Layer / Q000379:methods*; D004077:Digoxin / Q000097:blood* / Q000494:pharmacology / Q000652:urine; D006801:Humans; D011849:Radioactive Tracers; D012016:Reference Values; D012463:Saliva / Q000032:analysis; D012588:Scintillation Counting
D011849:Radioactive Tracers; D004077:Digoxin