Sodium and water transport in frog skin: effect of indomethacin.
The transepithelial transport of sodium, rubidium and water was studied in the frog skin in the presence of the cyclic endoperoxidase inhibitor indomethacin. Sodium transport was studied by measuring short circuit current and by 24Na radioactive isotope method; water transport was examined using the frog skin sac technique. Indomethacin produced the following changes. 1. The transepithelial potential difference and the intensity of short circuit current were decreased, while the resistance and conductance of the skin remained unaltered. 2. Passive transepithelial sodium transport from the inner to the outer side was enhanced. 3. Neither osmotic water transport nor passive rubidium transport were changed.


CONCLUSIONS
a) Indomethacin causes a selective increase of sodium permeability in the frog skin; b) The present results are in agreement with the view that the indomethacin may influence the rate of sodium transport in some tissues.
D000818:Animals; D001001:Anura; D001692:Biological Transport / Q000187:drug effects; D005260:Female; D007213:Indomethacin / Q000494:pharmacology*; D008297:Male; D009994:Osmolar Concentration; D011868:Radioisotopes; D012413:Rubidium / Q000378:metabolism; D012867:Skin / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D012979:Sodium Radioisotopes; D014867:Water / Q000378:metabolism*
D011868:Radioisotopes; D012979:Sodium Radioisotopes; D014867:Water; D012964:Sodium; D012413:Rubidium; D007213:Indomethacin