High speed autoradiography on brain tissue sections.
The application of high-speed autoradiography to histologic brain sections of in vivo [3H] thymidine labeled rats is reported. Low doses of isotope have been used. With Kodak NTB3 emulsion, specific nuclear labelling of the subependymal layer was reached after 48 h of exposure. With llford K2 emulsion, the maximal nuclear labelling was achieved within 20 days. The fitness of this technique for studying the nervous tissue kinetics is discussed.
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000033:anatomy & histology*; D051381:Rats; D013936:Thymidine; D013997:Time Factors; D014316:Tritium
D014316:Tritium; D013936:Thymidine