[Electron microscopic findings during hypothermic storage preservation of the liver. II. (author's transl)].
Porcine livers were preserved by simple hypothermic storage for 6 h using plasma-protein-fraction 5%, albumin 5%, Collins C2, and Ringer-lactate followed by a morphometric evaluation of the electron-microscopic changes. The morphometric parameters reflecting swelling of mitochondriae showed the most pronounced changes and were suited best for reproduction. The best result was achieved by using Collins C2-storage. The endoplasmic reticulum was found to be only slightly swollen in all preservation solutions investigated here, at the same time a degranulation of granular endoplasmic reticulum could be demonstrated. The increase in volume of lysosomes per unit-volume was not significant.
D000818:Animals; D004721:Endoplasmic Reticulum; D007036:Hypothermia, Induced; D008099:Liver / Q000473:pathology*; D008247:Lysosomes / Q000648:ultrastructure; D008854:Microscopy, Electron; D008930:Mitochondria, Liver / Q000648:ultrastructure; D008933:Mitochondrial Swelling; D009926:Organ Preservation / Q000379:methods*; D013552:Swine; D014021:Tissue Preservation / Q000379:methods*
Elektronenmikroskopische Befunde während hypothermer Lagerungskonservierung der Leber. II. Morphometrie der Hepatocyten.