Estimation of the p and m isomers of hydroxyphenylacetic acid in mouse brain by a gas chromatographic procedure: their regional distribution and the effects of some drugs.
The mouse brain contains 12.5 and 4.1 ng/g of p- and m-hydroxyphenylacetic acids, respectively. The hydroxyphenylacetic acids were isolated by chromatography on DEAE-Sephadex A-25 and quantitated as their pentafluoropropionyl and hexafluoropropanol esters by use of a gas chromatograph equipped with an electron-capture detector. The highest concentrations of p- or m-hydroxyphenylacetic acids were observed in the caudate nuclei (27.9 and 8.7 ng/g, respectively) and olfactory tubercles (20.2 and 5.3 ng/g, respectively). The identities of the p- and m-hydroxyphenylacetic acids were further confirmed as a consequence of the reductions observed following monoamine oxidase inhibition or the increases observed in the appropriate acid following the parenteral administration of p- or m-tyramine.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D001923:Brain Chemistry; D002421:Caudate Nucleus / Q000187:drug effects / Q000378:metabolism; D002849:Chromatography, Gas; D006719:Homovanillic Acid / Q000378:metabolism; D008297:Male; D051379:Mice; D009928:Organ Specificity; D010293:Pargyline / Q000494:pharmacology; D010648:Phenylacetates / Q000378:metabolism*; D014439:Tyramine / Q000378:metabolism / Q000494:pharmacology
D010648:Phenylacetates; C008070:4-hydroxyphenylacetic acid; D010293:Pargyline; C008069:3-hydroxybenzeneacetic acid; D006719:Homovanillic Acid; D014439:Tyramine