Oligonucleotide directed mutagenesis of the human beta-globin gene: a general method for producing specific point mutations in cloned DNA.
A nonadecanucleotide has been used both as a site specific mutagen to introduce a T leads to A transversion mutation in the human beta-globin gene cloned in pBR322 as well as a probe to screen transformed colonies for the desired mutant. The specificity of the oligonucleotide as a mutagen and as a hybridization probe provide a general method for producing site specific mutations in DNA cloned in plasmid vectors such as pBR322.
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism*; D005796:Genes / Q000187:drug effects*; D005914:Globins / Q000235:genetics*; D006801:Humans; D009153:Mutagens; D009154:Mutation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000494:pharmacology*; D010957:Plasmids
D004274:DNA, Recombinant; D009153:Mutagens; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D005914:Globins