Enzyme-linked immunosorbent assay for anti-histone antibodies and their presence in systemic lupus erythematosus sera.
We describe an enzyme-linked immunosorbent assay (ELISA) with adsorption of histones (total and fractions) on glass beads and saturation of excess sites with sheep serum. The anti-histone antibodies are detected with peroxidase conjugate and developed with Trinder's reagent which has great stability. This very sensitive method detects anti-histone antibodies in 53% of SLE patients and in virtually no other diseases. Positive reactions are observed only with total histones and fractions H1 and H2b.
D000042:Absorption; D000818:Animals; D000906:Antibodies; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006657:Histones / Q000276:immunology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009994:Osmolar Concentration; D012756:Sheep
D000906:Antibodies; D006657:Histones