Thin-layer chromatographic separation of conjugates of ursodeoxycholic acid from those of litho-, chenodeoxy-, deoxy-, and cholic acids.
Separation of the glycine and taurine conjugates of ursodeoxycholic acid from those of lithocholic acid, chenodeoxycholic acid, deoxycholic acid, and cholic acid by thin-layer chromatography is described. Thus, on running a silica gel G plate first in a solvent system of n-butanol-water 20:3 and then in a second solvent system of chloroform-isopropanol-acetic acid-water 30:20:4:1, all the above-mentioned conjugated bile acids are separated from one another. The application of this method to study the change in the biliary bile acid conjugation pattern in ursodeoxycholic acid-fed gallstone patients is described.
D002635:Chenodeoxycholic Acid / Q000031:analogs & derivatives*; D002855:Chromatography, Thin Layer / Q000379:methods; D003840:Deoxycholic Acid / Q000031:analogs & derivatives*; D005998:Glycine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D005999:Glycochenodeoxycholic Acid / Q000302:isolation & purification; D006000:Glycocholic Acid / Q000302:isolation & purification; D006002:Glycodeoxycholic Acid / Q000302:isolation & purification; D008095:Lithocholic Acid / Q000031:analogs & derivatives / Q000302:isolation & purification; D013655:Taurochenodeoxycholic Acid / Q000302:isolation & purification*; D013656:Taurocholic Acid / Q000302:isolation & purification; D013657:Taurodeoxycholic Acid / Q000302:isolation & purification; D013658:Taurolithocholic Acid / Q000302:isolation & purification; D014580:Ursodeoxycholic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification
D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; D006002:Glycodeoxycholic Acid; D013655:Taurochenodeoxycholic Acid; D013657:Taurodeoxycholic Acid; D013658:Taurolithocholic Acid; D013656:Taurocholic Acid; D008095:Lithocholic Acid; C031655:ursodoxicoltaurine; D005999:Glycochenodeoxycholic Acid; C024033:glycoursodeoxycholic acid; D014580:Ursodeoxycholic Acid; D006000:Glycocholic Acid; C027746:glycolithocholic acid; D005998:Glycine