Cultures enriched in Schwann-like cells from dissociated nerve segments of the adult cat.
Methods for producing Schwann cell-rich cultures from nerve segments of the adult cat were investigated. Efficient dissociation was achieved with a combination of collagenase and trypsin. Differential attachment of elements within the resulting suspension allowed production of Schwann cell-rich cultures derived by outgrowth from slowly attaching segments of degenerating myelin sheath tube containing Schwann-like cells. Rapid trypsinization during subculture further increased the proportion of Schwann-like cells in these cultures to 90% or more.
D000818:Animals; D002415:Cats; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005260:Female; D008854:Microscopy, Electron; D012583:Schwann Cells / Q000166:cytology*; D012584:Sciatic Nerve / Q000166:cytology*; D014459:Ulnar Nerve / Q000166:cytology*