A new, rapid and precise method for measuring the intra-cellular water content by gas chromatography and 3H-sucrose.
A new rapid method for measuring intra-cellular water content by gas chromatography and an isotopic (3H-sucrose) technique is described. Water content of circulating red cells was revealed to be 71.26 +/- 0.31%. Intra-cellular water content increased to 102.9% of its original value following addition of 3 volumes of acid-citrate-dextrose (ACD) to 20 volumes of blood.
D000328:Adult; D002849:Chromatography, Gas; D019343:Citric Acid; D004912:Erythrocytes / Q000032:analysis* / Q000187:drug effects; D005947:Glucose / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D007424:Intracellular Fluid / Q000032:analysis; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013395:Sucrose; D014316:Tritium; D014867:Water / Q000032:analysis*
D014867:Water; D014316:Tritium; C002113:acid citrate dextrose; D019343:Citric Acid; D013395:Sucrose; D005947:Glucose