Stimulation of collagen biosynthesis by platelet homogenate in various cell cultures.
Confluent cultures of Balb c/3T3, L-929 and PZ-32 cells were incubated at 37 degrees C for 16 h in the serum-free culture medium supplemented with ascorbic acid, beta-aminopropionitrile and (5(-3) H)proline. An addition of platelet homogenate to the culture medium increased significantly proline incorporation into proteins and synthesis of the hydroxyproline-containing, collagenase-sensitive proteins.
D000818:Animals; D001792:Blood Platelets / Q000502:physiology*; D002460:Cell Line; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D003470:Culture Media; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007739:L Cells / Q000378:metabolism; D051379:Mice; D011247:Pregnancy; D012867:Skin / Q000196:embryology
D003470:Culture Media; D003094:Collagen