Comparison of the oxidation of glutamine, glucose, ketone bodies and fatty acids by human diploid fibroblasts.
The contribution of glutamine, glucose, ketone bodies and fatty acids to the oxidative energy metabolism of human diploid fibroblasts ws studied. The rate of glutamine oxidation by fibroblasts was 98 nmol/h per mg cell protein compared to 2 nmol/h per mg cell protein or less for glucose, acetoacetate, D-3-hydroxybutyrate, octanoic acid and palmitic acid. Glucose inhibited glutamine oxidation by 85%, while the other substrates had no effect. Therefore, these cells meet their energy requirement almost solely by anaerobic glycolysis and glutamine oxidation.
D002460:Cell Line; D005227:Fatty Acids / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D007657:Ketone Bodies / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction; D012867:Skin / Q000378:metabolism*
D005227:Fatty Acids; D007657:Ketone Bodies; D005973:Glutamine; D005947:Glucose