Purification and properties of two superoxide dismutases from human placenta.
Copper and zinc-containing superoxide dismutase (Cu,Zn-SOD), and manganese-containing superoxide dismutase (Mn-SOD) were purified from human placenta by heat treatment at 70 degrees for 5 min, following precipitation with ammonium sulfate and column chromatographies. The molecular weights of Cu,Zn-SOD and Mn-SOD estimated by gel filtration were 32000 and 81000, respectively. From the result of SDS polyacrylamide gel electrophoresis, Cu,Zn-SOD consisted of two subunits with an equal molecular weight of 16000 and Mn-SOD consisted of four subunits with an equal molecular weight of 21000. One g-atom each of copper and zinc was contained in the subunit of Cu,Zn-SOD, and one g-atom of manganese was contained in the subunit of Mn-SOD. Immunological properties of superoxide dismutases showed that no organ specificity existed but that a species specificity obviously existed. The method of Laurell's rocket immunoelectrophoresis which can determine Cu,Zn-SOD or Mn-SOD on the basis of the immunological difference has been established. The contents of Cu,Zn-SOD and Mn-SOD in various human tissues were measured by this method.
D000917:Antibody Formation; D004355:Drug Stability; D005260:Female; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007122:Immunoelectrophoresis; D066298:In Vitro Techniques; D008670:Metals; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013482:Superoxide Dismutase / Q000276:immunology / Q000302:isolation & purification*
D008670:Metals; D013482:Superoxide Dismutase