Collagen and noncollagen protein synthesis in chick limb bud cells infected with a virus that causes osteoblastoma.
Infection of cultured stage 24 (day 4.5) chick limb cells (spot cultures) with myeloblastosis-associated virus 2-(osteo) (MAV-2(0)) produces cell density-dependent alterations of collagen and noncollagen protein synthesis over the course of 8 days in culture. Collagen synthesis in infected, low density cultures (100 k cells/spot) was maximal on day 4 and increased 12 fold compared with maximal values on days 5-6 for uninfected counterparts. The patterns of collagen synthesis were similar in uninfected or MAV-2(0)-infected cultures seeded at 200 (medium density) or 400 k cells (high density) per spot with peaks on days 5-6. Noncollagen protein synthesis (NCP) in infected, low density cultures was maximal on day 4 and increased 15.7 fold compared with maximal values on days 5-6 for uninfected counterparts. The patterns for NCP synthesis were similar for uninfected and MAV-2(0)-infected cultures seeded at medium density (peak on day 5). However, in MAV-2(0)-infected , high density cultures, NCP synthesis was maximal on day 4 and increased 2.9 fold compared with maximal values on days 5-6 for uninfected counterparts. Although kinetics of cell division and DNA synthesis were similar in uninfected and MAV-2(0)-infected limb bud cells, the virus may have diverse affects on different cell populations at the three cell densities employed in these experiments.
D000818:Animals; D009189:Avian Myeloblastosis Virus; D001859:Bone Neoplasms / Q000378:metabolism*; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D003094:Collagen / Q000096:biosynthesis*; D004247:DNA / Q000096:biosynthesis; D005121:Extremities / Q000196:embryology; D010017:Osteoma, Osteoid / Q000378:metabolism*; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D013936:Thymidine / Q000378:metabolism
D003094:Collagen; D004247:DNA; D011392:Proline; D013936:Thymidine