Streptomyces setonii: catabolism of vanillic acid via guaiacol and catechol.
Streptomyces setonii (strain 75Vi2) was grown at 45 degrees C in liquid media containing simple aromatic compounds as principal carbon sources. Thin-layer chromatography, UV spectrophotometry, and gas chromatography were used to show that S. setonii converted benzoic acid, guaiacol, and vanillic acid to catechol; p-hydroxybenzoic acid to protocatechuic acid; and m-hydroxybenzoic acid to gentisic acid. Presence of the ring-cleavage enzymes catechol 1,2-dioxygenase, protocatechuate 3,4-dioxygenase, and gentisate 2,3-dioxygenase was shown both by O2 uptake in ring-cleavage reactions catalyzed by cell-free extracts and by changes in UV spectra that indicated the presence of specific ring-cleavage products. A unique feature of this strain was its catabolism of vanillic acid by of guaiacol and catechol, using a pathway that had not been confirmed previously.
D001565:Benzoates / Q000378:metabolism; D050548:Catechol 1,2-Dioxygenase; D002396:Catechols / Q000378:metabolism; D049308:Dioxygenases; D006139:Guaiacol / Q000378:metabolism; D062385:Hydroxybenzoates / Q000378:metabolism*; D010105:Oxygenases / Q000378:metabolism; D011520:Protocatechuate-3,4-Dioxygenase / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism*; D014641:Vanillic Acid / Q000378:metabolism*
D001565:Benzoates; D002396:Catechols; D062385:Hydroxybenzoates; D006139:Guaiacol; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; D011520:Protocatechuate-3,4-Dioxygenase; C026247:gentisate 1,2-dioxygenase; D014641:Vanillic Acid