Aging in the AXC rat: equivalence of the rates of inactivation of l-ornithine decarboxylase and S-adenosyl-L-methionine decarboxylase in prostate of young and aged rats.
The incorporation of leucine into proteins of ventral and dorsolateral prostates of young (3- to 4-month-old) and aged (24- to 26-month-old) AXC rats was examined in vivo. We established that a single injection of cycloheximide (500 micrograms/100 g BW) maintained 91% (ventral) or 86% (dorsolateral) inhibition of protein synthesis in prostates of young AXC rats and 90% (ventral) or 88% (dorsolateral) inhibition of protein synthesis in prostates of aged AXC rats through at least 120 min post injection.  During cycloheximide-maintained inhibition of protein synthesis, the rate of inactivation of ventral prostate L-ornithine decarboxylase (ODC) or S-adenosyl-L-methionine decarboxylase (AMDC) activity and dorsolateral prostate AMDC activity was comparable in the prostates of young and aged AXC rats. The absence of an age-related change in the rate of inactivation of prostatic ODC or AMDC activity provides additional evidence that the age-related decrease in prostatic ODC and AMDC activities reported in the companion article may reflect altered androgen regulation of prostatic gene activity.
D012437:Adenosylmethionine Decarboxylase / Q000096:biosynthesis*; D000375:Aging; D000818:Animals; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D003513:Cycloheximide / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D011467:Prostate / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D051381:Rats; D011919:Rats, Inbred Strains
D003513:Cycloheximide; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase