Construction of bacterial plasmids containing sequences complementary to chicken alpha-tropomyosin mRNA.
Recombinant plasmids have been constructed with contain sequences complementary to the mRNA coding for skeletal muscle alpha-tropomyosin. These recombinants were detected initially using a selective cDNA probe and subsequently using a messenger RNA selection assay. alpha-TM plasmids hybridize to a singly mRNA species smaller than 18S ribosomal RNA and found only in skeletal muscle. Cross-hybridization with mRNA's coding for other tropomyosins could not be detected under normal conditions. However, under conditions of reduced stringency alpha- TM plasmids cross-hydridize with an RNA species in heart muscle which may code for cardiac tropomyosin.
D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics; D009132:Muscles / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012156:Reticulocytes / Q000378:metabolism; D014335:Tropomyosin / Q000235:genetics*
D004274:DNA, Recombinant; D009124:Muscle Proteins; D012333:RNA, Messenger; D014335:Tropomyosin