[Simple, highly specific procedures for radioimmunochemical estimation of hypophyseal protein hormones].
Immunosorbents, prepared by means of polymerization of antiserum proteins using chlorcarbonic acid isobutyl ester, were used to increased reproducibility and simplicity of the radioimmunochemical procedures for estimation of the estimation of the hypophyseal hormones. In the types of the radioimmunoassay (RIA) studied the immunosorbents were prepared by means of polymerization of the first and second antibodies. The universal immunosorbent, suitable to estimation of various antigens, was prepared from the second antibodies. The procedure enabled to use as the first antibodies the blood sera with comparatively low antibody titre. High specificity of the RIA procedures described was demonstrated in estimation of bovine hypophyseal hormones - lactogenous (LTH), somatotropic (STH) and beta-lipotropine. The calibration curves were linear in the concentration range from 2 to 20 ng/ml for LTH, and from 1 to 10 ng/ml for STH and beta-lipotropine. Estimation of LTH by means of the RIA procedure required 3 hrs, and of STH and beta-lipotropine - 16 hrs.
D000818:Animals; D002417:Cattle; D013006:Growth Hormone / Q000032:analysis; D007986:Luteinizing Hormone / Q000032:analysis; D008832:Microchemistry; D010902:Pituitary Gland / Q000032:analysis; D010907:Pituitary Hormones / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D008083:beta-Lipotropin / Q000032:analysis
D010907:Pituitary Hormones; D007986:Luteinizing Hormone; D013006:Growth Hormone; D008083:beta-Lipotropin
Prostye vysokospetsifechnye metody radioimmunokhimicheskogo opredeleniia belkovykh gormonov gipofiza.