Quantitative evaluation of neutrophilic chemotaxis in beagles.
A quantitative evaluation of neutrophilic chemotaxis was performed, using neutrophils from 12 healthy Beagles. Dextran sedimentation, differential centrifugation, and hypotonic lysis allowed separation of a neutrophil-rich cell suspension. Chemotaxis was studied, using blind-well chambers with 3-micrometers (APD) polycarbonate filters. A frozen (-80 C) bank of pooled normal canine serum was used as the chemotactant, and the chemotactant properties of the serum were destroyed by heat inactivation at 56 C for 30 minutes. Based on membrane and lower blind-well chamber solution neutrophil counts, a method was devised to distinguish between normal neutrophilic chemotaxis, normal neutrophilic chemotaxis with decreased neutrophilic adherence, and defective neutrophilic chemotaxis.
D000818:Animals; D002630:Chemotactic Factors / Q000032:analysis; D002634:Chemotaxis, Leukocyte; D004285:Dogs / Q000097:blood*; D005260:Female; D006801:Humans; D009504:Neutrophils / Q000502:physiology
D002630:Chemotactic Factors