The structure of chromatin synthesized in the presence of cycloheximide in Physarum polycephalum.
The effect of cycloheximide on protein and DNA synthesis and on the structure of chromatin was studied. Changes in the rate and extent of DNA synthesis in response to cycloheximide were highly variable in contrast to the extremely rapid and reproducible inhibition of protein synthesis. No differences in the rate of the release of acid-soluble products by nucleases and in the nature of the nucleoprotein particles were found in chromatin from plasmodia treated and non-treated with cycloheximide. It is concluded that, in Physarum polycephalum, unlike in higher animals, chromatin from the antibiotic-treated plasmodia is structurally indiscernible by the methods applied from normal chromatin.
D002843:Chromatin / Q000187:drug effects / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology*; D004271:DNA, Fungal / Q000096:biosynthesis; D005656:Fungal Proteins / Q000096:biosynthesis; D007700:Kinetics; D010804:Physarum / Q000187:drug effects / Q000378:metabolism*
D002843:Chromatin; D004271:DNA, Fungal; D005656:Fungal Proteins; D003513:Cycloheximide