[Properties of rat liver polyribosomes synthesizing transferrin].
A radioimmunological study of rat liver polyribosomes synthesizing transferrin was carried out. The 125I-labelled monospecific antibodies against transferrin interact with membrane-bound liver polyribosomes to form a specific antigen-antibody complex. No binding of 125I-antibodies against transferrin to free liver polyribosomes was observed. The transferrin polyribosomes contain 11-13 monoribosomes per 1 molecule of mRNA. An injection of cycloheximide to the animals results in an increase in the size of the transferrin polyribosomes up to 16 monoribosomes per 1 mRNA molecule. During translation of the liver polyribosomal fractions in a cell-free system of wheat embryos only the membrane-bound polyribosomes were capable to synthesize immunoprecipitated transferrin.
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002458:Cell Fractionation; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D010944:Plants / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism* / Q000648:ultrastructure; D014176:Protein Biosynthesis; D011863:Radioimmunoassay; D051381:Rats; D014168:Transferrin / Q000096:biosynthesis*; D014908:Triticum / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D014168:Transferrin
Kharakteristika poliribosom pecheni krysy, sinteziruiushchikh transferrin.