[In vitro studies of prolactinomas].
A few prolactinomas have been cultured into semipermanent cell lines. This transformation-like behavior is demonstrated. The tumor cells, obtained after transphenoidal surgery have been grown on extracellular matrix, using a mixture of Sephadex beads or a feeder layer of irradiated C.H.O. cells. The cells did survive to several sub-cultures and formed clones rapidly growing in soft agar. The PRL secretion evaluated through R.I.A. method declined with sub-culturing. A study of dopaminergic receptors on the same kind of material has shown the presence of those receptors and their persistence, even through sub-culturing.
D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D002999:Clone Cells; D006801:Humans; D010911:Pituitary Neoplasms / Q000378:metabolism* / Q000473:pathology; D011388:Prolactin / Q000378:metabolism*; D011954:Receptors, Dopamine / Q000378:metabolism
D011954:Receptors, Dopamine; D011388:Prolactin
Etudes in vitro des prolactinomes.