Improved separation of motile sperm in asthenospermia and its application to artificial insemination homologous (AIH).
A method is described for the separation of motile sperm from semen by "migration' into a diluent, providing a large area of interface between the semen and the diluent and resulting in a good yield of motile sperm (58%), with a mean percentage of motility of 95%. The method provides a specimen free of seminal plasma and debris and suitable for intrauterine insemination in less than two hours. The motile fraction was used for AIH treatment of 20 couples with a mean of 4 years' infertility, where the female factor had been excluded and the male was asthenospermic. After a mean of six cycles, three pregnancies were achieved. Improvement of the percentage of motility alone did not, in this study, give a significant improvement in conception rates when compared with accepted cycles with the placebo.
D000328:Adult; D002469:Cell Separation; D004958:Estradiol / Q000097:blood; D005186:Fallopian Tube Patency Tests; D005260:Female; D006801:Humans; D007315:Insemination, Artificial / Q000379:methods*; D007317:Insemination, Artificial, Homologous / Q000379:methods*; D008297:Male; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011388:Prolactin / Q000097:blood; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000473:pathology*
D011374:Progesterone; D004958:Estradiol; D011388:Prolactin