Somatomedin activity measured as sulphation factor in culture media from normal human liver and connective tissues explants. Effects of human growth hormone.
Normal human explants from liver and from connective tissues (aponeurosis or skin) incubated in vitro released sulphation activity measurable in chick embryo cartilage. Addition of human Growth Hormone (hGH) at physiological levels (10 ng/ml) increased the sulphation activity after 6 hours incubation time. Higher doses failed to increase the sulphation activity produced by connective tissues and decreased the sulphation activity produced by the liver.
D000818:Animals; D001681:Biological Assay; D002356:Cartilage / Q000378:metabolism; D002642:Chick Embryo; D003238:Connective Tissue / Q000378:metabolism*; D003470:Culture Media; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D008099:Liver / Q000378:metabolism*; D009924:Organ Culture Techniques; D013002:Somatomedins / Q000378:metabolism*
D003470:Culture Media; D013002:Somatomedins; D013006:Growth Hormone