Identification of beta-D-mannosylceramide in hepatopancreas of the fresh-water bivalve, Hyriopsis schlegelii.
A mannosylceramide was isolated by preparative thin-layer chromatography on a 3% borate-impregnated silica gel plate from a monohexosylceramide fraction of the hepatopancreas of the fresh-water bivalve, Hyriopsis schlegelii. It contained only mannose as the sugar component and the ceramide moiety contained mainly sphingosine and palmitic acid. Anomeric configuration of the sugar moiety was determined by enzymatic hydrolysis with beta-D-mannosidase. The concentration of this glycolipid was 5% of the total monohexosylceramide fraction of the hepatopancreas.
D000818:Animals; D002236:Carbohydrate Conformation; D002554:Cerebrosides / Q000302:isolation & purification*; D002855:Chromatography, Thin Layer; D008099:Liver / Q000032:analysis; D008358:Mannose / Q000032:analysis; D008974:Mollusca / Q000032:analysis*; D010169:Palmitic Acids / Q000032:analysis; D010179:Pancreas / Q000032:analysis; D013110:Sphingosine / Q000032:analysis
D002554:Cerebrosides; D010169:Palmitic Acids; D013110:Sphingosine; D008358:Mannose