Phospholipid metabolism of dog liver under hypoxic conditions induced by ligation of the hepatic artery.
Ischemic hypoxic liver was induced in dogs by ligation of the hepatic artery. About 67% of the dogs died of liver necrosis within 1 or 2 days (severe cases), and the rest survived (mild cases). In the severe cases, the decreases in the contents of total lipids, phospholipids and proteins of the liver after 24 h were 24, 46 and 12%, respectively, of the original values. The marked decrease in phospholipids was due to decreases in the microsomal and mitochondrial fractions. In the mild cases, similar but smaller decreases occurred and decrease of phospholipids occurred only in the microsomal fraction. The main phospholipids were choline and ethanolamine glycerophospholipids, and their molecular species were analyzed. In the severe cases, ligation resulted in relative increases in mono- and diene species and a decrease in polyene species. No increase in phospholipase activity was found at various times after ligation of the hepatic artery. Penicillin-treated dogs all survived and showed little decrease in liver phospholipids.
D000818:Animals; D004285:Dogs; D005227:Fatty Acids / Q000378:metabolism; D005997:Glycerylphosphorylcholine / Q000378:metabolism; D006499:Hepatic Artery / Q000502:physiology*; D000860:Hypoxia / Q000378:metabolism*; D007700:Kinetics; D008026:Ligation; D008099:Liver / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*
D005227:Fatty Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D005997:Glycerylphosphorylcholine