5 alpha-Dihydrotestosterone-induced uteroglobin synthesis in rabbit uterus is not inhibited by antiandrogen administration but is prevented by estradiol.
The physiological androgen, 5 alpha-dihydrotestosterone (DHT), enhances a progesterone-regulated protein (uteroglobin) synthesis in the rabbit uterus. In order to clarify the induction mechanism(s), rabbits were treated for 5 days with DHT alone or concomitantly with a nonsteroidal antiandrogen, RU 23908, or with different doses of estradiol. Uteroglobin content was measured in the uterine fluid by radioimmunoassay and uteroglobin mRNA activity in uterine tissues using cell-free translation in vitro. Uteroglobin induction elicited by DHT was inhibited by a small dose of estradiol, but not by antiandrogen. These results support the idea tha androgens bring about their action on uteroglobin synthesis via a mechanism involving uterine progesterone receptor.
D000726:Androgen Antagonists / Q000494:pharmacology*; D000818:Animals; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis*; D007093:Imidazoles / Q000494:pharmacology; D048289:Imidazolidines; D066298:In Vitro Techniques; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D011980:Receptors, Progesterone / Q000187:drug effects; D014598:Uteroglobin / Q000096:biosynthesis*; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism
D000726:Androgen Antagonists; D006023:Glycoproteins; D007093:Imidazoles; D048289:Imidazolidines; D012333:RNA, Messenger; D011980:Receptors, Progesterone; D013196:Dihydrotestosterone; D004958:Estradiol; C021277:nilutamide; D014598:Uteroglobin