Radioisotopic microassay of adenosine deaminase in human lymphocytes.
A radioisotopic method for a rapid assay of adenosine deaminase in human lymphocytes is proposed here. [2-3H] adenosine, as substrate, has been employed for the enzymatic assay. The products of reaction have been resolved by thin layer chromatography on PEI cellulose. The plates were developed with distilled water and inosine spots absorbing in the U.V. were eluted with 0.1 N HCl. The eluates obtained from the inosine spots were employed for radioactive measurements.
D000241:Adenosine / Q000378:metabolism; D000243:Adenosine Deaminase / Q000097:blood*; D006801:Humans; D007288:Inosine / Q000378:metabolism; D008214:Lymphocytes / Q000201:enzymology*; D008832:Microchemistry / Q000379:methods; D009700:Nucleoside Deaminases / Q000097:blood*
D007288:Inosine; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D000241:Adenosine