[Dynamics of metabolism of different compartments of amino acid pools in yeast cells].
The incorporation of [14C] into the amino acid pools located in different cell compartments of the yeast Candida tropicalis was studied. The rate of [14C]-labelled amino acids incorporation into the cytoplasm is 2 times higher than that in the vacuoles and 10 times higher than that in mitochondria. The amino acid pools under study are heterogenous in terms of generation rate: each of them contain at least two minor carbon pools, i.e. rapidly regenerating and relatively stable ones, whose ration is different. While the rapidly regenerating part of the amino acid pools of the protoplasts makes up to about 8.8% of total carbon pool, that of the mitochondrial pool makes up to 17% and the vacuolar one is only 2% of total carbon of the amino acid pools in these organelles.
D000596:Amino Acids / Q000378:metabolism*; D002175:Candida / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002451:Cell Compartmentation; D007700:Kinetics; D014617:Vacuoles / Q000378:metabolism
D000596:Amino Acids; D002250:Carbon Radioisotopes
Issledovanie dinamiki obmena prostranstvenno-razgranichennykh aminokislotnykh fondov drozhzhevoĭ kletki.