Preparation and characterization of high-specific activity radiolabeled 50 S measles virus RNA.
A method is described to radiolabel measles virus RNA for hybridization studies. Tritiated nucleosides were added to the media of measles virus infected Vero cells and negative-strand (genome) RNA with a specific activity of 6 X 10(5) c.p.m./microgram was purified from viral nucleocapsids. 50 S RNA was the sole RNA present in nucleocapsids and self-annealed to 50% due to the presence of 25% 50 S plus-strands (anti-genomes).
D000241:Adenosine / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D006151:Guanosine / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods*; D008459:Measles virus / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis / Q000302:isolation & purification; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D012367:RNA, Viral; D014316:Tritium; D006151:Guanosine; D000241:Adenosine; D014529:Uridine