Effect of hydrocortisone on long-term human marrow cultures.
In human long-term marrow cultures a relatively high concentration of hydrocortisone (10(-6) M or more) is necessary for the development and subsequent maintenance of some cellular components in the adherent cell layer. However, such concentrations of hydrocortisone seem to be inhibitory for the production of granulocyte/macrophage progenitor cells (GM-CFC). In further attempts to improve these cultures, therefore, it is important to maintain a balance between a concentration of hydrocortisone which is necessary for the development of a competent adherent layer and the concentration which is inhibitory to the production of GM-CFC.
D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D002478:Cells, Cultured; D003470:Culture Media; D006098:Granulocytes / Q000166:cytology; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D013997:Time Factors
D003470:Culture Media; D006854:Hydrocortisone