Malonaldehyde formation is not a suitable screening test to detect oxidation in human neutrophils.
The thiobarbituric acid (TBA) assay for measuring malonaldehyde (MDA) has been applied to many tissues as a simple means of detecting oxidative damage and prostaglandin synthesis. Human neutrophils, cells known to produce prostaglandins and toxic metabolites of oxygen, were studied to determine if this assay could provide a suitable, rapid, screening test to recognise states of metabolic activation, to monitor prostaglandin synthesis, or to identify the presence of oxidative damage. Malonaldehyde could not be detected after a variety of manipulations. Thus, it seems unlikely that the thiobarbituric acid assay, as performed here, will be useful for evaluating oxidative damage in human neutrophils.
D006801:Humans; D008314:Malonates / Q000032:analysis*; D008315:Malondialdehyde / Q000032:analysis*; D009504:Neutrophils / Q000378:metabolism*; D010084:Oxidation-Reduction; D013858:Thiobarbiturates / Q000378:metabolism
D008314:Malonates; D013858:Thiobarbiturates; D008315:Malondialdehyde