The binding of oestradiol-17beta to human spermatozoa - an electron microscope autoradiographic study.
The location of steroid binding sites with specificity for oestradiol-17beta at an ultrastructural level has been examined on human spermatozoa by electron microscope autoradiography. About 75% of the 438 gains produced by 3H-oestradiol-17beta, and counted on electron microscope autoradiographs, were found over the plasma membrane or within 1000 A of it. This study provides evidence suggesting that the plasma membrane is a site of action of oestradiol-17beta in human spermatozoa.
D001345:Autoradiography; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D013094:Spermatozoa / Q000378:metabolism* / Q000648:ultrastructure
D004958:Estradiol