Preservation of the fertilizing capacity of cock semen incubated in vitro at 41 C.
Cock spermatozoa were incubated for 6 and 24 hr at 41 C with minimum essential medium (MEM), tissue culture fluid (TCF) removed from tissue cultured cells and in the presence of tissue cultured cells (TCC). the TCC were obtained from whole 9-day-old chick embryos. Fertility following intravaginal artificial insemination was 75, 77, and 80% for semen incubated at 41 C for 6 hr in MEM, TCF, and TCC, respectively. Fertility of semen incubated for 24 hr was approximately 50% for all three treatment groups. The MEM alone was as beneficial as TCF or TCC in maintaining in vitro sperm survival. These observations provide a method for maintaining in vitro the fertilizing capacity (as evaluated by intravaginal insemination) of large numbers of cock spermatozoa under conditions of temperature comparable to those of the hen's oviduct.
D000818:Animals; D002645:Chickens / Q000502:physiology*; D005260:Female; D005298:Fertility; D006358:Hot Temperature; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*