Metabolism of tryptophan by Pseudomonas aureofaciens and its relationship to pyrrolnitrin biosynthesis.
Studies on the metabolism of tryptophan in Pseudomonas aureofaciens ATCC 15926 revealed different metabolic routes for the L- and D-isomer besides the biosynthetic pathway for pyrrolnitrin synthesis. L-Tryptophan catabolism follows the aromatic route via anthranilic acid. Tryptophan 2,3-dioxygenase were induced by L-tryptophan. Kynureninase and anthranilate 1,2-dioxygenase were induced by L-tryptophan, L-kynurenine and anthranilic acid. Anthranilate 1,2-dioxygenase was absent from a mutant strain of P. aureofaciens ATCC 19526 which produced about 30-fold increased amounts of pyrrolnitrin. The Km values of tryptophan 2,3-dioxygenase and kynureninase did not differ substantially between the two strains. Kynurenine 3-monooxygenase, 3-hydroxyanthranilate 3,4-dioxygenase, tryptophanase and indolyl-3-alkane alpha-hydroxylase activities were not detected. D- and L-tryptophan were converted to indole-3-acetic acid. This additional catabolic pathway was well as tryptophan racemase activity was constitutive and present in both strains.
D000935:Antifungal Agents / Q000096:biosynthesis*; D004790:Enzyme Induction; D005990:Glycerol / Q000378:metabolism; D007210:Indoleacetic Acids / Q000378:metabolism; D007737:Kynurenine / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism*; D011764:Pyrrolnitrin / Q000096:biosynthesis*; D014364:Tryptophan / Q000378:metabolism*; D014366:Tryptophan Oxygenase / Q000378:metabolism; D062367:ortho-Aminobenzoates / Q000378:metabolism
D000935:Antifungal Agents; D007210:Indoleacetic Acids; D062367:ortho-Aminobenzoates; D007737:Kynurenine; D014364:Tryptophan; D014366:Tryptophan Oxygenase; D011764:Pyrrolnitrin; D005990:Glycerol