[Quantitative analysis of the heterogeneity of rat brain mitochondria in isolated fractions and in situ].
Heterogeneity of mitochondrial fractions in solutions of sucrose, sucrose and EDTA, mannitol and EDTA, and in Krebs-Ringer solutions with and without glucose was assayed by morphometry. Heterogeneity of mitochondria in varied solutions significantly differed from that in situ. Statistical analysis of mitochondria in isolated fractions and in situ as well as an analysis of the literary data allowed a conclusion that the mitochondrial system in the nerve cell is threadlike rather than discrete in shape. It has been established that heterogeneity of mitochondria in vitro depends on the conditions of tissue homogenization and isolation of the mitochondrial fraction.
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D002458:Cell Fractionation / Q000379:methods; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure; D051381:Rats; D013347:Subcellular Fractions
Kolichestvennyĭ analiz geterogennosti mitokhondriĭ mozga krys v izolirovannoĭ fraktsii i in situ.