Specific anti-thyroxine antisera induced by thyroxine sensitized liposomes.
Specific anti-thyroxine rabbit antisera were generated from complete Freund's adjuvant with liposomes consisting of sphingomyelin, cholesterol, dicetylphosphate and 5-N-thyroxine-2,4-dinitrophenyl-phosphatidylethanolamine (T4-Dnp-PE). Spin membrane immunoassay technique was used to measure the sensitivity and specificity of these antisera. Addition of 10 to 70 ng of L-thyroxine produced significant inhibition of immune lysis. Addition of L-3,3',5-triiodothyronine (T3) up to 800 ng showed no cross reaction.
D000588:Amines / Q000378:metabolism; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002784:Cholesterol / Q000378:metabolism; D006168:Guinea Pigs; D007106:Immune Sera / Q000494:pharmacology*; D008081:Liposomes / Q000276:immunology*; D011817:Rabbits; D013109:Sphingomyelins / Q000378:metabolism; D013113:Spin Labels; D013974:Thyroxine / Q000276:immunology*
D000588:Amines; D007106:Immune Sera; D008081:Liposomes; D013109:Sphingomyelins; D013113:Spin Labels; D002784:Cholesterol; C009317:stearylamine; D013974:Thyroxine