31P-Nuclear magnetic resonance and freeze-fracture electron microscopic studies on reconstituted bacteriorhodopsin vesicles.
Bacteriorhodopsin has been reconstituted into egg-phosphatidylcholine vesicles by various methods. The resulting preparations have been analyzed on density gradients and by freeze-fracture electron microscopy. The homogeneity of the vesicle preparations and the light-induced intravesicular pH changes have been studied by 31P-NMR, using glucose 6-phosphate as pH probe. It is concluded that bacteriorhodopsin is incorporated in the inside-out mode in vesicles up to about 100 nm. Above this diameter, more or less random insertion takes place.
D001436:Bacteriorhodopsins; D002338:Carotenoids; D003902:Detergents; D005614:Freeze Fracturing; D006217:Halobacterium; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy; D008854:Microscopy, Electron; D017830:Octoxynol; D011092:Polyethylene Glycols; D011487:Protein Conformation
D003902:Detergents; D008081:Liposomes; D002338:Carotenoids; D011092:Polyethylene Glycols; D001436:Bacteriorhodopsins; D017830:Octoxynol