Fractionation of plasma proteins with PEG.
Plasma proteins were fractionated with polyethylene glycol (PEG-4000) and the effects of pH, ionic strength and temperature were examined. Fractionation with 15% PEG was the most suitable for obtaining a precipitate containing mainly IgG, and a supernatant containing chiefly albumin. These fractions may then be purified further.
D001798:Blood Proteins; D005591:Chemical Fractionation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G; D009994:Osmolar Concentration; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012709:Serum Albumin; D013696:Temperature
D001798:Blood Proteins; D007074:Immunoglobulin G; D012709:Serum Albumin; D011092:Polyethylene Glycols