Separative determination of ascorbic acid and erythorbic acid by high-performance liquid chromatography.
High-performance liquid chromatography (HPLC) was applied to the determination of ascorbic acid (AsA) and erythorbic acid (ErA). The apparatus was a Shimadzu model LC-2P Liquid Chromatograph equipped with a UV detector set at 254 nm. The separation was achieved on a LiChrosorb-NH2 column which was pre-treated with 0.1 M ammonium monophosphate solution using a mixture of acetonitrile, acetic acid and water (87:2:11, v/v) as an eluant. The HPLC method has the following advantages: AsA and ErA are quantitated after being distinctly separated, analysis time per one sample is short, and AsA or ErA levels as low as 1.0 X 10(-2) microgram are detectable. Recovery experiments with dehydro-AsA and dehydro-ErA, involving reduction with H2S, give satisfactory results.
D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003124:Colorimetry; D013237:Stereoisomerism
D000975:Antioxidants; C005056:isoascorbic acid; D001205:Ascorbic Acid