L(-)-10-Formyltetrahydrofolate is the cofactor for glycinamide ribonucleotide transformylase from chicken liver.
It is shown that L(-)-10-formyltetrahydrofolate serves as the cofactor for glycinamide ribonucleotide transformylase from chicken liver. The utilization of L(-)-10-formyl-H4folate was not previously recognized, because L-(+)-10-formyl-H4folate is an excellent competitive inhibitor of the enzyme, Ki = 0.75 +/- 0.07 microM, and historically the transformylase assay was carried out with a mixture of diastereomers. The results are discussed in relation to the utilization of L(+)-5,10-methenyl-H4folate.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D002645:Chickens; D005575:Formyltetrahydrofolates / Q000494:pharmacology*; D019877:Hydroxymethyl and Formyl Transferases; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D051026:Phosphoribosylglycinamide Formyltransferase; D013056:Spectrophotometry, Ultraviolet; D013763:Tetrahydrofolates / Q000494:pharmacology*
D005575:Formyltetrahydrofolates; D013763:Tetrahydrofolates; D019877:Hydroxymethyl and Formyl Transferases; D051026:Phosphoribosylglycinamide Formyltransferase; D000217:Acyltransferases