A new method for detecting haemoglobins with decreased stability: tryptic digestibility test.
A method based on measuring the tryptic digestibility of haemoglobin has been elaborated for the detection of mutations affecting the conformation of the haemoglobin molecule. By the aid of this technique not only haemoglobins that prove labile by other procedures can be detected, but such mutants can also be discovered which confer no decreased stability as reflected by clinical and conventional laboratory examinations. The method is suitable for tackling questions of theoretical or basic research interest, whilst in a simplified form it can be applied as a routine laboratory test.
D006441:Hemoglobin A; D006448:Hemoglobin J; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D008722:Methods; D009154:Mutation; D011487:Protein Conformation; D014357:Trypsin / Q000494:pharmacology*
D006454:Hemoglobins; D006448:Hemoglobin J; D006441:Hemoglobin A; D014357:Trypsin