Oxidation of meso-diaminosuccinic acid, a possible natural substrate for D-aspartate oxidase.
meso-Diaminosuccinic acid, a natural antagonist of aspartic acid, is a good substrate for beef kidney D-aspartate oxidase. The oxygen consumption and the ammonia production are in good agreement with the stoichiometry of a typical oxidative deamination. The deamination involves only one of the two amino groups of diaminosuccinate, and is followed by decarboxylation of the first reaction product, 2-amino-3-oxosuccinic acid. By condensation of the compounds thus originating from this latter, pyrazine 2,5-dicarboxylic acid and pyrazine 2,6-dicarboxylic acid are formed. DL-Diaminosuccinic acid is not oxidized by D-aspartate oxidase, nor does it inhibit the enzyme activity.
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000599:Amino Acids, Diamino / Q000378:metabolism*; D000818:Animals; D001224:Aspartic Acid; D002417:Cattle; D050804:D-Aspartate Oxidase; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D013053:Spectrophotometry; D013379:Substrate Specificity
D000599:Amino Acids, Diamino; D001224:Aspartic Acid; C031934:diaminosuccinic acid; D000594:Amino Acid Oxidoreductases; D050804:D-Aspartate Oxidase