Effective cell support by repeat plateletpheresis of related donors on the IBM 2997 cell separator.
Forty-three non-HLA-matched donors provided 83 plateletpheresis products for 20 thrombocytopenic patients during a six-month period. The platelet product yield was 4.2 x 10(11) collected in 90 min. There was 26% cellular depletion of donor platelets and 20% depletion of donor lymphocyte per procedure. Males had a significantly greater lymphocyte depletion: 24% compared with 14% for females (P less than 0.05). There was no significant cellular depletion seen in 16 donors who underwent from two of a maximum of nine procedures. For these 16 donors, the time interval between procedures was a minimum of three days and a maximum of 100 days. Twelve refractory oncology patients received 49 plateletpheresis transfusions from 26 related donors. The mean corrected one-hour posttransfusion platelet increment was 18,300, and the mean corrected 20-hour posttransfusion platelet increment was 13,000. The results indicate that non-HLA-typed related plateletpheresis donors can safely undergo multiple procedures with the IBM 2997 Cell Separator and can effectively support their thrombocytopenic relatives who are unresponsive to random donor platelets.
D001782:Blood Donors; D002469:Cell Separation; D005190:Family; D005260:Female; D006801:Humans; D008297:Male; D017713:Platelet Transfusion; D010983:Plateletpheresis; D013921:Thrombocytopenia / Q000628:therapy