Two structurally distinct and independently regulated idiotypic families associated with the A/J response to azophenylarsonate.
At least two distinct subpopulations of A/J anti-azophenylarsonate (Ars) antibodies can be defined on the basis of serological cross-reactivity and sequence homology of heavy and light chains. The most frequently occurring idiotype family, IdCR, was initially identified by a rabbit antiserum and is present only in A/J mice. Examination of several randomly selected hybridoma proteins which failed to express IdCR-associated determinants revealed a second set of closely related molecules. The second idiotype family, Id36-60, is recognized by a rabbit antiserum prepared against the hybridoma protein 36-60. It is present in practically all A/J and BALB/c, but not B10. A Ars immune sera. On average, A/J immune sera contain approximately one-fifth as much Id36-60 as IdCR. A/J mice can be suppressed for subsequent expression of either idiotype by neonatal injection of anti-IdCR or anti-Id36-60 antisera. Suppression of IdCR does not affect the production of Id36-60 and vice versa. Together, these idiotype systems provide two independent markers for monitoring network regulation of the anti-Ars antibody response in A/J mice.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D001391:Azo Compounds / Q000276:immunology*; D003429:Cross Reactions; D006825:Hybridomas / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D008815:Mice, Inbred Strains; D011817:Rabbits; D010132:p-Azobenzenearsonate / Q000276:immunology*
D000906:Antibodies; D001391:Azo Compounds; D010132:p-Azobenzenearsonate