Effect of energy inhibitors on cell surface diphtheria toxin receptor numbers.
The effects of various metabolic inhibitors on cell-surface receptors for diphtheria toxin were studied. Incubation of Vero cells at 37 degrees C with fluoride, azide, salicylate, and, to a lesser degree, with deoxyglucose resulted in an apparent loss of receptors for the toxin. With some drugs the loss was rapid (half-time approximately 8-9 min) while with others considerably longer time periods were required (half-time greater than or equal to 40 min). The loss of toxin receptors could also be induced by the drugs at 4 degrees C. Washing out the drugs and incubating the cells at 37 degrees C led to a complete restoration of receptors to control numbers. Restoration did not occur if cells were incubated at 4 degrees C. The regain of binding capacity was not dependent on RNA or protein synthesis. It is suggested that metabolic inhibitors may block a continuous process required to maintain toxin receptors in a functional state at the cell surface.
D019297:2,4-Dinitrophenol; D000818:Animals; D001386:Azides / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002522:Chlorocebus aethiops; D003847:Deoxyglucose / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D004167:Diphtheria Toxin / Q000378:metabolism*; D005459:Fluorides / Q000494:pharmacology; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D007668:Kidney; D007700:Kinetics; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic / Q000187:drug effects / Q000378:metabolism*; D012459:Salicylates / Q000494:pharmacology; D020156:Salicylic Acid; D014475:Uncoupling Agents / Q000494:pharmacology
D001386:Azides; D004140:Dinitrophenols; D004167:Diphtheria Toxin; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D012459:Salicylates; D014475:Uncoupling Agents; D003847:Deoxyglucose; D020156:Salicylic Acid; D019297:2,4-Dinitrophenol; D005459:Fluorides