Effect of methylglyoxal on rat liver lysosome-vacuolar apparatus.
Methylglyoxal (MeG) administration to rats doesn't cause any change in liver lysosome integrity; on the other hand, lysosome osmotic fragility appears highly increased at short times after the treatment. Concomitantly, a significant decrease of the residual latent activity of acid phosphatase (RLA = activity which cannot be unmasked by extensive hypo-osmotic shock) occurs. Cycloheximide (CHM), when administered shortly before MeG, prevents both the changes in RLA and in osmotic fragility. These results point to a big stimulation of autophagy in rat liver by MeG.
D000135:Acid Phosphatase / Q000378:metabolism; D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000648:ultrastructure*; D008247:Lysosomes / Q000187:drug effects*; D008297:Male; D009940:Organoids / Q000187:drug effects*; D009996:Osmotic Fragility; D011765:Pyruvaldehyde / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051381:Rats; D014617:Vacuoles / Q000187:drug effects*
D000447:Aldehydes; D011765:Pyruvaldehyde; D003513:Cycloheximide; D000135:Acid Phosphatase