Separation of hemopoietic stem cells of low self-renewal capacity from mouse bone marrow.
A simple technique to concentrate hemopoietic stem cells (colony-forming unit in spleen, CFUs) on a ficoll-triosil gradient, permitting an enrichment of the stem cells from mouse bone marrow by a factor of 10, was developed. The proportion of the enriched cells engaged in DNA synthesis was shown to be around 40%. The self-renewal capacity of the enriched stem cells was significantly lower than that of the total stem cells in normal mouse bone marrow. The separated stem cells may correspond to CFUs-2.
D000818:Animals; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000096:biosynthesis; D005362:Ficoll; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008297:Male; D008794:Metrizoic Acid; D051379:Mice; D013154:Spleen / Q000166:cytology
D005362:Ficoll; D004247:DNA; D008794:Metrizoic Acid