Radio-ligand immunoassay for human hemoglobin variants.
A quantitative method is described for the individual assay of human hemoglobin variants occurring singly or in mixture. The hemoglobin to be assayed is bound to specific antibody; the immune complex is attached to protein A-containing S. aureus and removed from the mixture. The hemoglobin thus isolated is quantified by its ability to bind radiolabeled haptoglobin. The technique is accurate and distinguishes among the 4 hemoglobins tested, namely Hb A, S, C and F. It has the advantage over conventional radioimmunoassay that a single probe, radiolabeled haptoglobin, is needed for the specific assay of any hemoglobin.
D014644:Genetic Variation; D006454:Hemoglobins / Q000032:analysis* / Q000235:genetics; D006801:Humans; D011869:Radioligand Assay / Q000379:methods*
D006454:Hemoglobins