Radioimmunoassay of oestriol in the urine of non-pregnant women.
A radioimmunoassay for oestriol in the urine of non-pregnant women is described. Urine is hydrolysed rapidly at room temperature with an E. coli glucuronidase preparation and extracted prior to a simple radioimmunoassay procedure. An antiserum raised against oestriol-6-(O-carboxymethyl) oxime bovine serum albumin is used with (3H)-oestriol as tracer. Free and antibody-bound fractions are separated by partition between ammonium sulphate solution and scintillant without centrifugation. The accuracy and precision of the assay are satisfactory. It gave values approximately 80% of those obtained for total oestrogens by a fluorimetric method.
D000645:Ammonium Sulfate; D004355:Drug Stability; D004964:Estriol / Q000031:analogs & derivatives / Q000276:immunology / Q000652:urine*; D004967:Estrogens / Q000652:urine; D005260:Female; D005966:Glucuronidase; D006801:Humans; D010091:Oximes / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods
D004967:Estrogens; D010091:Oximes; C002913:estriol-6-(O-carboxymethyl)oxime; D005966:Glucuronidase; D004964:Estriol; D000645:Ammonium Sulfate