[Variability in the DNA content in the progeny of the clonogenic cells from transplantable rat rhabdomyosarcoma].
Transplantable rat rhabdomyosarcoma RA-2 selected for affinity to lung tissue was cloned in vivo, and DNA contents of interphase nuclei was cytophotometrically measured in progenies of 121 clonogenic cells implanted in the lungs. Clone populations have shown a high karyotypic heterogeneity, karyotypic structure being unchanged from the 16th to the 28th selection cycles investigated. Karyotypic heterogeneity is reproduced due to the high rate of genome mutations in the progenies of clonogenic cells (11.10-2-14.10-2 per cell per generation). Cells with increased numbers of chromosomes arose 3 fold more frequently than cells with decreased chromosome numbers, tumor cell mutability being non-equal in different clones.
D000818:Animals; D002999:Clone Cells / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D005787:Gene Frequency; D007621:Karyotyping; D009154:Mutation; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000032:analysis; D010783:Photometry; D051381:Rats; D012208:Rhabdomyosarcoma / Q000032:analysis*
D004273:DNA, Neoplasm
Izmenchivost' soderzhaniia DNK v potomstvakh klonogennykh kletok perevivnoĭ rabdomiosarkomy krys.