Relative contributions of parenchymal and non-parenchymal (sinusoidal) liver cells in the uptake of chylomicron remnants.
The relative contributions of parenchymal cells and non-parenchymal (sinusoidal) cells to the in vivo hepatic uptake of chylomicron remnants was measured 30 min after intravenous injection into rats. The chylomicron remnants were labeled with [3H]leucine, which was almost exclusively present in apolipoprotein B. The isolated non-parenchymal cells (a mixture of Kupffer cells and endothelial cells) contained 6.7 times more apolipoprotein B radioactivity per mg cell protein than the isolated parenchymal cells. It was calculated that the contributions of non-parenchymal and parenchymal liver cells to the total hepatic uptake of chylomicron remnants are 35% and 65%, respectively.
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001053:Apolipoproteins / Q000378:metabolism; D001055:Apolipoproteins B; D002914:Chylomicrons / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008196:Lymph / Q000032:analysis; D008297:Male; D008722:Methods; D051381:Rats; D014316:Tritium
D001053:Apolipoproteins; D001055:Apolipoproteins B; D002914:Chylomicrons; D014316:Tritium; C004420:4-aminopyrazolo(3,4-d)pyrimidine; D007930:Leucine; D000225:Adenine