Purification of a nuclease from human serum.
The purification procedure for a nuclease from human serum is described. It includes ammonium sulfate precipitation, chromatography on DEAE-Sephadex and on Sephacryl-S 200, and preparative electrophoresis. The enzyme purified about 2000-fold, is homogeneous in a sodium dodecyl sulfate electrophoretic system, where it has a mol. wt of 78,000. The pH optimum lies around pH 6.5; it is a sugar-nonspecific endonuclease.
D003851:Deoxyribonucleases / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D007700:Kinetics
D003851:Deoxyribonucleases