Neurite outgrowth from murine sensory ganglia cultured in a serumless medium.
Trigeminal and dorsal root ganglia were excised from mouse embryos of 10 and 18 days in utero age, respectively, and grown in tissue culture. A quantitative method was used to assess the extent of neurite outgrowth from the explants after 24 and 48 h in culture. Outgrowth from ganglia grown in a serumless medium was compared with that from ganglia in a medium supplemented with serum. There was no significant difference between the extent of outgrowth in either medium after 24 h in culture; however, after 48 h there was significantly greater outgrowth in the serum-supplemented medium.
D000818:Animals; D002454:Cell Differentiation; D003470:Culture Media; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology*; D008297:Male; D009115:Muridae; D009474:Neurons / Q000166:cytology; D011247:Pregnancy; D014276:Trigeminal Nerve / Q000166:cytology*
D003470:Culture Media