Attachment of human pancreatic tumor cell lines to collagen in vitro.
The attachment to purified collagens of three cell lines established from human pancreatic carcinomas was investigated. PANC-1 and CAPAN-1 cells attached to type I, III, and IV collagens in the absence of fetal bovine serum. MIA PaCa-2 cells, on the other hand, attached only to type IV collagen. In the case of MIA PaCa-2 cells, the attachment occurs more slowly and to a lesser extent. Increasing concentrations of fetal bovine serum had no effect on the attachment of PANC-1 and CAPAN-1 cells to the collagens. However, the attachment of MIA PaCa-2 cells to all the collagen types was greatly enhanced by 10% fetal bovine serum. This enhancement was shown to be due to the fibronectin present in the serum.
D001769:Blood; D002448:Cell Adhesion; D002460:Cell Line; D003094:Collagen / Q000378:metabolism*; D003470:Culture Media; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D013997:Time Factors
D003470:Culture Media; D005353:Fibronectins; D003094:Collagen