Nonhistone chromatin proteins HMG-14 and HMG-17 bind preferentially to single-stranded DNA.
Proteins extracted from chicken erythrocyte chromatin with 0.35 M NaCl were subjected to sequential chromatography on columns containing immobilized double-stranded and single-stranded DNA's. Two-dimensional electrophoresis of protein fractions revealed that HMG-14 and HMG-17 are among the proteins that are retained by the single-stranded DNA column in 0.2 M NaCl/l mM Tris-Cl (pH 7.5) after having failed to be retained by the double-stranded column under the same conditions. That suggests that those two proteins possess preferential affinity for single-stranded DNA. Further evidence for that was provided by chromatography of purified HMG-14 and of purified HMG-17 on single-stranded and double-stranded DNA columns. We discuss the possible relevance of our results to suggested functions of HMG-14 and HMG-17.
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000378:metabolism; D002846:Chromatography, Affinity; D002868:Chromosomal Proteins, Non-Histone / Q000097:blood* / Q000302:isolation & purification; D004247:DNA; D004277:DNA, Single-Stranded / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004277:DNA, Single-Stranded; D004247:DNA