Identification of auto-antigens of the human sperm membrane.
Glycoproteins of human sperm membranes were labelled by a galactose oxidase-tritiated-sodium borohydride technique and detergent solubilized. A glycoprotein-enriched-fraction was obtained by lectin affinity chromatography. Subsequently, antigens in this fraction were isolated from the glycoprotein mixture by indirect immunoprecipitation and analysed in SDS-polyacrylamide gel electrophoresis. With some human sera with sperm agglutinating and immobilizing auto-antibodies at least three polypeptide chains were isolated, one with a molecular weight of about 41,000 and two with molecular weights of about 77,000. Other sera with equally strong agglutination and immobilization did not reveal any peaks in SDS-gel electrophoresis. Absorption studies using sequential indirect immunoprecipitation showed that the former group of sera independently of their mode of agglutination reacted with the same antigen(s).
D000941:Antigens / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000302:isolation & purification*; D001324:Autoantigens / Q000302:isolation & purification*; D002462:Cell Membrane / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006384:Hemagglutination; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D008297:Male; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; D013094:Spermatozoa / Q000166:cytology*
D000941:Antigens; D000954:Antigens, Surface; D001324:Autoantigens; D006023:Glycoproteins; D007106:Immune Sera; D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein