Jack bean urease (EC 3.5.1.5). III. The involvement of active-site nickel ion in inhibition by beta-mercaptoethanol, phosphoramidate, and fluoride.
Interaction of beta-mercaptoethanol with urease produces large, rapid and fully reversible spectral changes in that part of the electronic absorption spectrum which is associated with the tightly bound nickel ions. The spectrophotometrically determined value of the dissociation constant of the beta-mercaptoethanol-urease complex (0.9 +/- 0.05 mM at pH 7.12 and 25 degrees C) is in agreement with the Ki (0.72 +/- 0.26 mM) for beta-mercaptoethanol acting as a competitive inhibitor in the hydrolysis of urea. This constitutes direct evidence that the nickel in jack bean urease is at the active site. Inhibition of urease by phosphoramidate is slowly achieved and slowly reversed, and upon reactivation of the isolated phosphoramidate-urease complex, phosphoramidate is regenerated in good yield. Spectrophotometric experiments indicate that phosphoramidate binds to nickel ion in urease. Competition with beta-mercaptoethanol was used to determine a dissociation constant (1.23 +/- 0.10 mM at pH 7.12 and 25 degrees C) for a fluoride-evidence is presented which indicates that in the presence of urea, a ternary complex (fluoride-urea-urease) is formed.
D000577:Amides / Q000494:pharmacology*; D001665:Binding Sites / Q000187:drug effects; D007887:Fabaceae; D005459:Fluorides / Q000494:pharmacology*; D007700:Kinetics; D008623:Mercaptoethanol / Q000494:pharmacology*; D009532:Nickel / Q000378:metabolism*; D010756:Phosphoric Acids / Q000494:pharmacology*; D010944:Plants / Q000201:enzymology*; D010946:Plants, Medicinal; D013053:Spectrophotometry; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000037:antagonists & inhibitors* / Q000378:metabolism
D000577:Amides; D010756:Phosphoric Acids; D008623:Mercaptoethanol; D009532:Nickel; D014508:Urea; C011067:phosphoramidic acid; D014510:Urease; D005459:Fluorides