Measurement of mucosal blood flow by assay of absorption of 125I from the intestinal lumen.
A method which utilized the absorption of 125I as a measure of intestinal mucosal blood flow was developed. In the pentobarbital anesthetized dog a segment of mid-jejunum was isolated from adjacent vasculature and perfused with 125I solution. Superior mesenteric artery flow, blood flow to the isolated segment of intestine and 125I absorption were measured. Changes in 125I absorption were found to correlated closely with changes in blood flow (r = 0.95). Using an autoradiographic technique 125I absorption was found to be localized to the intestinal mucosa.
D000818:Animals; D004285:Dogs; D005260:Female; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000098:blood supply*; D007457:Iodine Radioisotopes; D008297:Male; D008638:Mesenteric Arteries / Q000502:physiology; D012039:Regional Blood Flow
D007457:Iodine Radioisotopes