Fourier analysis of isolated rat heart electrocardiographs as a measure of viability after cooling to - 22 degrees C with the aid of a cryoprotective agent.
The viability of isolated rat hearts cooled to - 22 degrees C with the aid of a cryoprotective agent, ethylene glycol, has been assessed by a novel technique of comparing the Fourier analysed spectrum of the electrocardiogram before and after cooling. The frequency spectrum of the electrocardiogram of a good isolated heart being retrograde perfused at 37 degrees C, contains 3 peaks with the second harmonic dominant is power. A damaged heart contains higher harmonics and additional components.
D000818:Animals; D003451:Cryoprotective Agents / Q000494:pharmacology; D004562:Electrocardiography; D005026:Ethylene Glycols / Q000494:pharmacology; D005583:Fourier Analysis; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D007036:Hypothermia, Induced / Q000009:adverse effects*; D066298:In Vitro Techniques; D051381:Rats; D014022:Tissue Survival / Q000187:drug effects
D003451:Cryoprotective Agents; D005026:Ethylene Glycols