Sister-chromatid exchange induction by polycyclic aromatic hydrocarbons in an intact cell system of adult rat-liver epithelial cells.
Adult rat-liver epithelial cell lines possess intrinsic metabolic capability for the biotransformation of xenobiotics and thus, are sensitive to a broad spectrum of mutagens/carcinogens in a mutagenesis assay at the hypoxanthine-guanine phosphoribosyl transferase locus. To provide another end-point of biological significance in these lines, we have investigated the application of adult rat-liver epithelial cell line 18 in a sister-chromatid exchange assay. Significant dose-dependent increases in the sister-chromatid exchange frequency occurred when liver cells were exposed to benzo[a]pyrene and 7,12-dimethylbenz[a]anthracene. A weak but positive response was elicited by benz[a]anthracene. The present observations thus confirm the capacity of these cells to generate genotoxic metabolites from activation-dependent mutagens/carcinogens and indicate a relationship between the production of mutations and sister-chromatid exchanges by polycyclic aromatic hydrocarbons.
D000818:Animals; D002460:Cell Line; D002842:Chromatids / Q000187:drug effects*; D003434:Crossing Over, Genetic; D008099:Liver; D009152:Mutagenicity Tests / Q000379:methods*; D011083:Polycyclic Compounds / Q000494:pharmacology*; D051381:Rats; D012854:Sister Chromatid Exchange
D011083:Polycyclic Compounds