Composition and immunochemical properties of glycoproteins with anti-B agglutinin activity isolated from Euonymus sieboldiana seeds.
Five major glycoproteins with anti-B agglutinin activity were isolated from seeds of Euonymus Sieboldiana by a procedure based on precipitation with ammonium sulphate, Sepharose 4B gel filtration, CM- and DEAE-Sepharose chromatography and Sephacryl S-200 gel filtration. The purified glycoproteins each gave a single symmetrical peak on Sephacryl S-200 gel filtration with elution volumes corresponding to molecular weights of approximately 15,000 to 130,000, each forming a single precipitin line on gel diffusion plates with anti-E. Sieboldiana antibody. These anti-B glycoproteins were rich in acidic amino acids without cysteine and methionine and contained about 8--36% carbohydrate, of which galactose, arabinose and glucose were the predominant sugars, with small amounts of glucosamine, rhamnose, fucose, xylose, mannose and ribose. The most anti-B active lectin agglutinated human B red blood cells at a concentration of 2 micrograms/ml and was strongly inhibited by melibiose. The three other lectins with anti-B agglutinin activity, however, were not inhibited by any galactose-containing glycosides.
D000017:ABO Blood-Group System / Q000276:immunology*; D000596:Amino Acids / Q000032:analysis; D000906:Antibodies / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000302:isolation & purification; D006801:Humans; D037102:Lectins / Q000302:isolation & purification; D008970:Molecular Weight; D037121:Plant Lectins; D010944:Plants; D012639:Seeds
D000017:ABO Blood-Group System; D000596:Amino Acids; D000906:Antibodies; D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins