The metabolism of N-acetyl-2-aminofluorene to a mutagen in L5178Y/TK+/-mouse lymphoma cells.
Direct treatment of L5178Y mouse lymphoma TK+/- cells with N-acetyl-2-aminofluorene (AAF) from two commercial sources produced small, but reproducible increases in mutant frequency over background in the absence of exogenous microsomal enzymes. Unlike most direct-acting mutagens which typically produce regular, dose-dependent increases in mutant frequency; AAF treatment caused very slight dose-related increases or a saturation phenomenon which could be overcome by increased exposure time. Direct mutagenicity following prolonged (24h) exposure was confirmed when a third highly purified (99.9%) AAF sample was tested. Microsomal enzyme analyses of disrupted L5178Y cell preparations revealed negligible benzo[a]pyrene hydroxylase but measurable AAF-N-hydroxylase activity. These data demonstrate that L5178Y mouse lymphoma cells are capable of limited metabolism of AAF to an active mutagen.
D015073:2-Acetylaminofluorene / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D004305:Dose-Response Relationship, Drug; D007940:Leukemia L5178 / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism*; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens / Q000378:metabolism*; D013997:Time Factors
D009153:Mutagens; D015073:2-Acetylaminofluorene