Immuno-electron microscopical features of in vivo antibody binding during pemphigus blister formation. Revelation with a new technique: peroxidase-labeled protein A.
Pemphigus autoantibodies, bound in the epidermis during different stages of acantholysis, were demonstrated with a new technique for immuno-electron microscopy. Peroxidase-labeled Protein A was used as a new and specific tracer for tissue-bound antibodies of the IgG-type. Advantages were: (1) A small molecular weight of the tracer, (2) a rapid tissue penetration, and (3) shortened incubation times, thus better preserved tissue fine structures. Unspecific adsorption in tissues and on cells was found to be comparatively low.
D000051:Acantholysis; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000032:analysis*; D004817:Epidermis / Q000276:immunology* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008854:Microscopy, Electron; D008875:Middle Aged; D010392:Pemphigus / Q000276:immunology* / Q000473:pathology; D010544:Peroxidases; D013205:Staphylococcal Protein A
D001323:Autoantibodies; D013205:Staphylococcal Protein A; D010544:Peroxidases