A simple method for the collection of cells from cerebrospinal fluid for electron microscopy.
A simple membrane filter technique permits the collection of cells from cerebrospinal fluid for electron microscopy. The method is universally applicable for the concentration of small number of cells from free-floating cell suspensions and has several advantages: (1) Only a small fluid sample (1 ml) is needed. (2) Excellent cell preservation is obtainable. (3) High cell recovery rates are achieved. (4) Quantitative classification of cell types is possible.
D002555:Cerebrospinal Fluid / Q000166:cytology*; D005374:Filtration / Q000379:methods; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*