High resolution R- and G-banding on the same preparation.
A simple method using bromodeoxyuridine (BrdU), for both cell synchronization and incorporation into replicating DNA is described. Many prophasic and prometaphasic mitoses were observed, and due to the probable blocking at different times of the cell cycle, very good R-banding and G-banding were obtained on the preparation.
D001973:Bromodeoxyuridine / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D002871:Chromosome Banding; D004261:DNA Replication / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D008938:Mitosis
D001973:Bromodeoxyuridine