Detection of anti-tumor immunity in man by the indirect macrophage adherence inhibition assay using guinea pig peritoneal cells as indicator cells.
Supernatants obtained after incubation of tumor patient lymphocytes with the homologous tumor-associated antigen (TAA) elicit adherence inhibition in normal guinea pig peritoneal cells. From 37 tested patients with different carcinomas, the supernatants of 30 caused more than 10 per cent adherence inhibition (breast cancer 11/11, lung cancer 7/8, colon cancer 6/7, stomach cancer 6/11). Besides 40 healthy blood donors were tested to a panel of TAA. There was a reactivity more than 10 per cent only in 2 cases. The supernatants of 14 patients with nonmalignant disorders produced adherence inhibition more than 10 per cent in 2 cases. By incubation of tumor patient lymphocytes with the human encephalitogenic protein the supernatants of 19/27 patients elicited a positive reaction. The results suggest that anti-tumor immunity can be detected in an indirect heterologous adherence inhibition system.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology; D001202:Ascitic Fluid / Q000166:cytology; D006168:Guinea Pigs; D006801:Humans; D007104:Immune Adherence Reaction; D008264:Macrophages / Q000276:immunology*; D009369:Neoplasms / Q000276:immunology*
D000951:Antigens, Neoplasm