The presence within single K-562 cells of erythropoietic and granulopoietic differentiation markers.
The continuous cell line K-562, derived from a patient with CML in blast crisis, was examined for markers of granulopoietic (My-1) and erythropoietic (spectrin) differentiation, using specific antibodies detected by indirect immunofluorescence. Both markers were seen, and in 10%--30% of cells, both were present in the same cells. In contrast, the continuous leukemic line HL-60 and KGI contained My-1 only. Controls consisted of colonies in culture containing both granulopoietic and erythropoietic cells (CFU-GEMM). In these, My-1 was seen only in granulopoietic cells and spectrin in erythropoietic cells. The suggestion is advanced that genes coding for differentiation markers are expressed abnormally in K-562.
D000954:Antigens, Surface; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D004920:Erythropoiesis; D006098:Granulocytes / Q000166:cytology*; D006410:Hematopoiesis; D006801:Humans; D011961:Receptors, Fc; D013049:Spectrin / Q000276:immunology / Q000494:pharmacology
D000954:Antigens, Surface; D011961:Receptors, Fc; D013049:Spectrin