Complete amino acid sequence of mouse liver metallothionein-II.
The complete amino acid sequence of thionein-II, one of the two major mouse liver thionein components, was determined. The main fragmentation of thionein-II, which consists of 61 amino acid residues, was accomplished by digesting the S-[14C]-carboxymethylated protein and the cyanogen bromide-treated oxidized protein with trypsin. The peptides obtained by papain digestion of S-[14C]carboxymethylated thionein-II were used to align the major tryptic peptides. The sequence was determined by a combination of automated and manual Edman degradation techniques. Remarkable structural homology is observed in mouse thionein-I, mouse thionein-II, and thioneins from man and horse.
D000595:Amino Acid Sequence; D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D055598:Chemical Phenomena; D002621:Chemistry; D008099:Liver / Q000032:analysis* / Q000187:drug effects; D008667:Metalloproteins; D008668:Metallothionein; D051379:Mice / Q000378:metabolism*; D010446:Peptide Fragments
D008667:Metalloproteins; D010446:Peptide Fragments; D002104:Cadmium; D008668:Metallothionein