Structure and conformation of human pancreatic carboxyl-ester hydrolase.
Human pancreatic carboxyl-ester hydrolase is a glycoprotein with a molecular weight of 100 000 and a high content in carbohydrate, 20%. Sedimentation studies indicate that the molecule resembles an ellipsoid. The results of hydrodynamic and vacuum-ultraviolet circular dichroism investigation allow one to propose a model of the carboxyl-ester hydrolase three-dimensional structure. The enzyme belongs to the "all beta" class of proteins; about 54-60% of its residues are in beta-sheets and in beta-turns, most probably forming the surface of an ellipsoid. A similarity with prealbumin structure is proposed and a comparison with structure of other pancreatic enzymes is presented.
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D002265:Carboxylic Ester Hydrolases; D002942:Circular Dichroism; D006801:Humans; D008970:Molecular Weight; D010189:Pancreatic Juice / Q000201:enzymology*; D011487:Protein Conformation
D000596:Amino Acids; D002241:Carbohydrates; D002265:Carboxylic Ester Hydrolases