A simple method for measuring total calcium in small tissue samples.
A simple and accurate method for determining total calcium in small tissue samples using aequorin is described. The method involves measuring the calcium-induced luminescence of aequorin with the tritium channel of a liquid scintillation counter. The sensitivity of the method and specificity of the method for calcium have been determined and, theoretically, this method could determine calcium in tissue samples weighing 3 mg or as little as 2 nmol of calcium could be measured.
D000331:Aequorin; D000818:Animals; D002118:Calcium / Q000032:analysis*; D005260:Female; D066298:In Vitro Techniques; D008722:Methods; D011817:Rabbits; D012588:Scintillation Counting; D013054:Spectrophotometry, Atomic; D014599:Uterus / Q000032:analysis
D000331:Aequorin; D002118:Calcium