[Staining with naphthol yellow S. 2. Study of the reversibility of the protein-dye binding reaction].
In the present work we studied the reversibility of the binding reaction of Naphthol Yellow S to proteins using a Testing Automatic System Leitz. We presently think that the NY-S staining procedure, can be used as a reliable quantitative method for the determination of nuclear and cytoplasmic proteins only under standardized conditions: the ratio R = [Dye-] / [R-NH+3] must be more greater than 10; for staining periods shorter than 45 min, the slides, at equilibrium, must be mounted in dyeing solution; for quantitative determinations using irreversible bound dye, the staining period must be significantly longer than 45 min (see above) and differentiated in running water for several hours.
D000818:Animals; D002478:Cells, Cultured; D004396:Coloring Agents; D006224:Cricetinae; D003412:Cricetulus; D007668:Kidney / Q000032:analysis*; D008722:Methods; D009282:Naphthalenesulfonates; D011485:Protein Binding
D004396:Coloring Agents; D009282:Naphthalenesulfonates; C009824:naphthol yellow
Colorazione con giallo naftolo S. II. Studio della reversibilita' della reazione colorante-proteine.