Morphological study of virus-like particles in two transplantable tumours from BDX rats.
Virus-like particles were found in two transplantable tumours, Sp56 and Sp6, from BDX rats. Sp56, a neurogenic sarcoma, contains abundant C-type particles in all stadia nof morphogeneis. This tumour reacts with anti-Friend leukaemia virus gp70 and anti-Rauscher leukaemia virus p30 sera. Sp6, a fibrosarcoma, has abundant virus-like particles in the cytoplasm, very often associated with centrioles or basal bodies of a cilium. These particles consist of two concentric shells with a diam. of 60 to 65 nm. Released particles were found outside the cell with a diam. of 85 to 100 nm characterized by an envelope and an eccentrically located electron-dense nucleoid, surrounded by an intermediate layer. These virus-like particles show no cross-reaction with antisera against murine C- or B-type particles, but show ultrastructural similarity with virus particles recently described in Chinese hamster cells and in mouse cell lines infected with two retrovirus isolates from South-East Asian mice.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D003593:Cytoplasm / Q000382:microbiology; D005354:Fibrosarcoma / Q000382:microbiology*; D005622:Friend murine leukemia virus / Q000276:immunology; D009368:Neoplasm Transplantation; D009455:Neurofibroma / Q000382:microbiology*; D010673:Pheochromocytoma / Q000382:microbiology*; D051381:Rats; D011925:Rauscher Virus / Q000276:immunology; D012190:Retroviridae / Q000276:immunology / Q000648:ultrastructure*; D014771:Virion / Q000648:ultrastructure*
D000956:Antigens, Viral