Lipid metabolism in peripheral nerve cell culture (rich in Schwann cells) from normal and trembler mice.
A culture of peripheral nerve cells, very rich in Schwann cells, was developed from sciatic nerve. In both normal and Trembler, typical spindle-shaped cells were seen; most of the cells were surrounded by basement membrane-like material (predominantly in-between adjacent cells). In Trembler cells, cultivated in the presence of labelled acetate, the fatty acids were slightly altered; phosphatidylcholine was slightly reduced and phosphatidylethanolamine increased. Sulfatides were increased four times.
D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis; D050356:Lipid Metabolism; D051379:Mice; D008818:Mice, Neurologic Mutants; D008854:Microscopy, Electron; D010743:Phospholipids / Q000096:biosynthesis; D012583:Schwann Cells / Q000378:metabolism* / Q000648:ultrastructure; D012584:Sciatic Nerve / Q000378:metabolism* / Q000648:ultrastructure; D013045:Species Specificity
D010743:Phospholipids; D002784:Cholesterol