Assay of trimethoprim, sulfadiazine and its N4-acetyl metabolite in biological fluids by normal-phase high-performance liquid chromatography.
A normal-phase high-performance liquid chromatographic method was developed to determine the concentration of timethoprim, sulfadiazine and its N4-acetyl derivative in human serum and urine. The unchanged compounds and the metabolite are extracted in organic solvent by a single extraction. The method is accurate and sensitive and suited for pharmacokinetic studies in man.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D011743:Pyrimidines / Q000494:pharmacology; D013411:Sulfadiazine / Q000097:blood* / Q000494:pharmacology / Q000652:urine; D013997:Time Factors; D014295:Trimethoprim / Q000097:blood* / Q000494:pharmacology / Q000652:urine
D011743:Pyrimidines; D013411:Sulfadiazine; C023507:tetroxoprim; D014295:Trimethoprim