A simplified procedure for purification of bovine growth hormone on Sephacryl S-200 superfine.
The isolation and purification of bovine growth hormone from pituitary glands by a shorter and efficient sequence of stages which utilizes the Reichert approach (Reichert, 1975) is described. The major innovations are: 1) precipitation in 2 M ammonium sulfate at pH 7.9 instead of pH 7.0 to obtain the extract designated as C-2 by Reichert and 2) early gel-chromatography on Sephacryl S-200 of the C-2 extract. The bGH obtained has higher specific activity than the standard reference hormone NIH-GH-B16. Electrophoretic and size homogeneity is investigated by isoelectric focusing and sodium dodecyl sulfate electrophoresis.
D000180:Acrylic Resins; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D013006:Growth Hormone / Q000302:isolation & purification*; D007525:Isoelectric Focusing
D000180:Acrylic Resins; C023049:sephacryl S 200; D013006:Growth Hormone