[Isolation and some properties of Hageman factor and its active fragment from human blood].
A procedure of isolation of the Hageman factor from human blood plasma, including ion-exchange chromatography on QAE-Sephadex A-50 and preparative disc-electrophoresis is proposed. The active fragment of the Hageman factor was obtained by contact activation of the latter followed by repeated disc-electrophoresis. The peculiarities of the Hageman factor activation by trypsin were studied. The conditions for maximal activation of prekallikrein by the Hageman factor and its active fragment were selected. It was found that the kallikrein formed therupon possesses a higher thermal lability than the trypsin-activated prekallikrein.
D002852:Chromatography, Ion Exchange; D004589:Electrophoresis, Disc; D004789:Enzyme Activation; D005174:Factor XII / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D010446:Peptide Fragments / Q000097:blood / Q000302:isolation & purification*
D010446:Peptide Fragments; D005174:Factor XII
Poluchenie i nekotorye svoĭstva faktora khagemana i ego aktivnogo fragmenta iz syvorotki krovi cheloveka.