Analysis of the phospholipid of the nuclear envelope and endoplasmic reticulum of liver cells by high pressure liquid chromatography.
A method is described for the separation and analysis of phospholipids from rat-liver nuclear envelope and endoplasmic reticulum. The procedure employs a liquid environment, to which antioxidants can be added, and results in separation of NL, PE, PI, PS, and PC in 99% purity in 12 min; analytical columns and a radial compression system may be employed. The procedure results in phospholipids with a large proportion of highly unsaturated fatty acids; some differences in fatty acid distributions were found when nuclear envelope phospholipid fractions were compared with the corresponding fractions from endoplasmic reticulum.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004721:Endoplasmic Reticulum / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D008099:Liver / Q000032:analysis*; D008297:Male; D009685:Nuclear Envelope / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D051381:Rats
D005227:Fatty Acids; D010743:Phospholipids