Comparative titration of Anaplasma marginale antibodies by card agglutination and complement-fixation tests.
The card agglutination test for anaplasmosis was improved so that bovine serum could be titrated for Anaplasma antibodies. The modified test was validated when its results on 1 set of serum samples were compared with the results furnished by the complement-fixation test on the same set. The samples tested were from cows inoculated with an Anaplasma vaccine or with either 1 of 2 virulent strains of Anaplasma marginale. A correlation coefficient of 0.60 was calculated between the values of the titers furnished by the 2 tests, and regression equations were calculated and used to estimate the serum titer in 1 test from a titer measured in the other. The results indicate that the simple card agglutination test can yield titers comparable with those of the complement-fixation test.
D000372:Agglutination Tests; D000709:Anaplasma / Q000276:immunology*; D000712:Anaplasmosis / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D003168:Complement Fixation Tests; D005260:Female
D000907:Antibodies, Bacterial