Immunoglobulin characterization by bacterial absorption of antibrain antibodies in multiple sclerosis.
Complement-fixing (CF) antibrain antibodies are frequently found in serum and CSF in multiple sclerosis (MS). They represent several specificities and appear to be synthesized on both sides of the blood-brain barrier. Five sera and six CSF samples from 11 MS patients, representing 6 different specificities of antibodies, were absorbed with a series of bacterial strains with affinity for various immunoglobulins. Reactivity with brain preparations was eliminated by absorption with the following IgG absorbents: Staphylococcus aureus strain Cowan I, group A streptococcus strain A R1, and group G streptococcus strain G 148, but not by absorption with strains with low or no affinity for IgG. The results indicate in all tested samples the IgG1 and/or IgG2 nature of the antibrain antibodies.
D000042:Absorption; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D001921:Brain / Q000276:immunology*; D003168:Complement Fixation Tests; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D009103:Multiple Sclerosis / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*; D013291:Streptococcus / Q000276:immunology*
D000906:Antibodies; D007074:Immunoglobulin G