The rapid preparation of synaptosomes, using a vertical rotor.
A rapid method for the preparation of isolated nerve terminals (synaptosomes) is described. The material, prepared using a vertical rotor, is compared biochemically with material prepared by conventional techniques. The synaptosomes possess many characteristics expected of isolated nerve terminals, including noradrenaline release and transport against a concentration gradient.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D002540:Cerebral Cortex / Q000648:ultrastructure*; D008928:Mitochondria / Q000648:ultrastructure; D009186:Myelin Sheath / Q000648:ultrastructure; D009419:Nerve Tissue Proteins / Q000032:analysis; D009638:Norepinephrine / Q000032:analysis; D051381:Rats; D012701:Serotonin / Q000032:analysis; D013574:Synaptosomes / Q000648:ultrastructure*
D009419:Nerve Tissue Proteins; D012701:Serotonin; D009638:Norepinephrine