[Effect on freezing on hydroxylation in isolated hepatocytes].
Rapid freezing of hepatocytes down to--196 degrees was accompanied by a distinct impairment in microsomal oxidation and by complete uncoupling of respiration and oxidative phosphorylation in mitochondria. In the course of multistep freezing activity of the microsomal oxidation system constituted 70.3% with ethylene glycol (EG) and 78% - with dimethyl sulfoxide (DMSO), while the functional activity of mitochondria was maintained by 30% and 40% if EG DMSO were used, respectively.
D000818:Animals; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005026:Ethylene Glycols / Q000494:pharmacology; D005615:Freezing; D006900:Hydroxylation; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D010085:Oxidative Phosphorylation; D051381:Rats
D005026:Ethylene Glycols; D004121:Dimethyl Sulfoxide
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