Coronavirus JHM: a virion-associated protein kinase.
Coronavirus JHM contains six major proteins, one of which, the 60 000 mol. wt. nucleocapsid protein pp60, is phosphorylated. In JHM-infected cells ip 60K, the intracellular precursor to pp60 is also phosphorylated. Associated with purified JHM virions is a protein kinase which will phosphorylate pp60 and a variety of exogenous substances in vitro. The enzyme has the characteristics of a cyclic nucleotide-independent protein kinase. Both the in vivo reaction and the enzyme activity in vitro transferred the gamma-phosphate of ATP to serine residues on the nucleocapsid protein.
D002213:Capsid / Q000032:analysis / Q000378:metabolism*; D003332:Coronaviridae / Q000032:analysis / Q000201:enzymology*; D007700:Kinetics; D008970:Molecular Weight; D010750:Phosphoproteins / Q000032:analysis; D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis; D011494:Protein Kinases / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D014771:Virion / Q000201:enzymology*
D010750:Phosphoproteins; D014764:Viral Proteins; D010768:Phosphoserine; D011494:Protein Kinases