[Cell wall polysaccharide structure in the food protein yeast, Candida spec. H. II. Characterization of phosphate binding to the mannan-protein-phosphate complex and identification of phosphodiester-bound mono- oligosaccharides].
The proteophosphomannan (PPM) obtained by extraction with citrate buffer, purification by the cetavlon method and gel filtration contains a high proportion of diesterified phosphate groups between the C-6 of a side chain mannose and another glycosidically bound carbohydrate. Mild acid hydrolysis cleaved the phosphate diester linkages to yield mono- and oligosaccharide fractions. Chemical analysis and 1H-NMR studies demonstrated the heterogeneity of the oligosaccharide fractions containing alpha(1,2)-, alpha(1,3)- and alpha(1,6)-linked manno-oligosaccharides. All repeated PPM preparations contained mono-, tri- and heptasaccharides as the phosphate bound main compounds. Galactose, arabinose, fucose, and rhamnose of the monosaccharide fraction and tetra-, penta- and hexasaccharides were not found in all preparations.
D002175:Candida / Q000032:analysis*; D002236:Carbohydrate Conformation; D002473:Cell Wall / Q000032:analysis; D005656:Fungal Proteins / Q000032:analysis*; D008351:Mannans / Q000032:analysis*; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis; D010710:Phosphates / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*
D005656:Fungal Proteins; D008351:Mannans; D009005:Monosaccharides; D009844:Oligosaccharides; D010710:Phosphates; D011134:Polysaccharides
Struktur der Zellwandpolysaccharide in der Futtereiweiss-Hefe Candida spec. H. II. Charakterisierung der Bindung des Phosphats am Mannan-Protein-Phosphate-Komplex und Identifizierung der als Phosphodiester gebundenen Mono- und Oligosaccharide.