Characterization of DNA polymerases from the mammary gland of pregnant rabbit.
1. The nuclear extract from the mammary gland of pregnant rabbit was shown to contain DNA polymerases alpha, beta and gamma. Polymerases alpha and gamma were found also in the mitochondria-free cytoplasmic fraction. 2. The nuclear enzymes were partially purified, separated by sucrose-density-gradient centrifugation, and identified on the basis of their template-primer utilization, sedimentation coefficients and sensitivity to N-ethylmaleimide. 3. Catalytic properties of the activated-DNA-dependent DNA polymerases alpha and beta were also described.
D000818:Animals; D002417:Cattle; D002457:Cell Extracts / Q000032:analysis; D002467:Cell Nucleus / Q000201:enzymology*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003600:Cytosol / Q000201:enzymology*; D004277:DNA, Single-Stranded; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification*; D005033:Ethylmaleimide / Q000494:pharmacology; D005260:Female; D008321:Mammary Glands, Animal / Q000201:enzymology* / Q000648:ultrastructure; D011247:Pregnancy; D011817:Rabbits; D013950:Thymus Gland
D002457:Cell Extracts; D004277:DNA, Single-Stranded; D004259:DNA-Directed DNA Polymerase; D005033:Ethylmaleimide