Evaluation of chemically induced cytogenetic lesions in rabbit oocytes. I. The test system and the effects of streptonigrin.
An appropriate method for evaluating transmissible genetic damage in female germ cells is presented. Analysis of karyotype preparations from preimplantation (6- day) rabbit embryos for consistent structural chromosome abnormalities is described as a sensitive way in which these determinations can be made. A table prepared from summarized research reports shows that less than 1 in 2000 rabbit blastocysts karyotyped thus far has a consistent structural chromosome abnormality. In the new data presented in this study, none of the 90 control blastocysts karyotyped had consistent structural chromosome abnormalities while 13 of 278 from female rabbits treated with streptonigrin (NSC-45383) before conception did. These findings demonstrate that karyotype analysis for consistent chromosome lesions in preimplantation rabbit embryos from females exposed to chemical clastogens can serve as a sensitive and quantitative means of estimating effects on oocytes. The results of the study also show that the consistent structural chromosome lesions in the blastocyst karyotypes are what would be predicted from the mode of action of streptonigrin and the segregation of chromosomes during meiosis. The relative efficiency of this system based on the rabbit as compared to another recently described system bases on the hamster is also discussed.
D000818:Animals; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D005260:Female; D007621:Karyotyping; D009152:Mutagenicity Tests; D009153:Mutagens; D009865:Oocytes / Q000187:drug effects*; D010063:Ovum / Q000187:drug effects*; D011817:Rabbits; D013308:Streptonigrin / Q000494:pharmacology
D009153:Mutagens; D013308:Streptonigrin