Thin-layer chromatography of hippuric and m-methylhippuric acid in urine after mixed exposure to toluene and xylene.
The separation of hippuric and m-methylhippuric acid as toluene and m-xylene metabolites present in urine of people exposed simultaneously to toluene and xylene is described. Chloroform was used for hippuric and m-methylhippuric acid extraction. Satisfactory separation of these metabolites was obtained on TLC plates covered with silica gels and developed in chloroform acetic acid-water (4:1:1);p-dimethylaminobenzaldehyde in acetic acid anhydride was applied to develop the colour. The sensitivity of the method was 6 micrograms hippuric acid per 1 ml urine and recovery was 100% (+/- 1).
D002855:Chromatography, Thin Layer / Q000379:methods*; D004781:Environmental Exposure; D006626:Hippurates / Q000652:urine*; D006801:Humans; D007202:Indicators and Reagents; D014050:Toluene / Q000378:metabolism*; D014992:Xylenes / Q000378:metabolism*
D006626:Hippurates; D007202:Indicators and Reagents; D014992:Xylenes; C031422:4-methylhippuric acid; D014050:Toluene; C030514:hippuric acid