Autofluorescence in the identification of myocardial infarcts.
A method is described for demonstrating myocardial necrosis by changes in the autofluorescence of routine hematoxylin and eosin or hematoxylin and eosin-phloxine stained sections using a standard microscope with an epifluorescence attachment. Regions of necrosis fluoresce with a brilliant yellow color clearly distinguishable from the dull olive-green to red-brown fluorescence of the neighboring viable cardiac muscle.
D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D009203:Myocardial Infarction / Q000175:diagnosis*