[Kinetic description and a model of the correction mechanism of bifunctional DNA-polymerases].
Bifunctional DNA-polymerases catalysed matrix-dependent synthesis of DNA chains is accompanied by the correction of non-correct nucleotides incorporation through exonuclease excision. To study the models of the correction mechanism a method of stationary graphs was used. This method gives possibility to deduce easily the equations which describe the frequency of mistakes in the growing DNA chain as a function of DNA-polymerase activity. Two mechanisms of correction (mechanism of immediate correction and mechanism of following correction) are proved on the base of simple models of DNA chain biosynthesis on a homopolymer matrix with the participation of correct and non-correct substrates. Effect of kinetic parameters, characterizing the inclusion and elimination processes of correct and non-correct nucleotides, on the replication fidelity was analysed. One of two investigated models was chosen on the base of known experimental data.
D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D007700:Kinetics; D046911:Macromolecular Substances; D008433:Mathematics; D008954:Models, Biological
D046911:Macromolecular Substances; D004259:DNA-Directed DNA Polymerase
Kineticheskoe opisanie i modelirovanie korrektiruiushchego mekhanizma bifunktsional'nykh DNK-polimeraz.