Simple high-performance liquid chromatographic method for the analysis of 9-(2-hydroxyethoxymethyl)guanine (acyclovir) in human plasma and urine.
Acyclovir, a new antiviral drug for human use, could not be solvent extracted and was retained poorly by reversed-phase (RP) columns. Drug in urine and plasma (deproteinised) could be chromatographed successfully as an ion-pair (with heptanesulphonic acid) on RP-18 columns. The validated method had the required sensitivity for a wide variety of clinical situations including metabolic and pharmacokinetic studies.
D000212:Acyclovir; D000998:Antiviral Agents / Q000097:blood* / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006147:Guanine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D006801:Humans; D011863:Radioimmunoassay; D012016:Reference Values
D000998:Antiviral Agents; D006147:Guanine; D000212:Acyclovir