[Effect of cortisone on free and bound RNA polymerase levels in rat liver cell nuclei].
Free and template-engaged RNA-polymerase activities were measured in nuclei from control and cortisone-stimulated rat livers. Both types of nuclei contain free and engaged alpha-amanitin sensitive (RNA-polymerase II) and insensitive (RNA-polymerase II) activities. In nuclei isolated after cortisone injection (1.5 and 3 hours) the stimulation of RNA-polymerase I in engaged form is observed. Unlike it, the higher activity of RNA-polymerase II in the free form is observed after cortisone induction, the activity of bound form being unchanged.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D003348:Cortisone / Q000494:pharmacology*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008297:Male; D011485:Protein Binding; D012318:RNA Polymerase I / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism*; D012320:RNA Polymerase III / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D014158:Transcription, Genetic
D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I; D012320:RNA Polymerase III; D003348:Cortisone
Vliianie kortizona na uroven' svobodnykh i sviazannykh RNK-polimeraz v iadrakh kletoko pecheni krys.