Saturated and unsaturated 1-O-alkyl-2-O-acetoyl-sn-glycero-3-phosphocholines derived from ratfish liver oil: effect on human leukocyte migration.
A mixture of 1-O-alkyl-2-O-acetoyl-sn-glycero-3-phosphocholines containing saturated alkyl moieties and a mixture of such compounds with saturated and unsaturated alkyl moieties, prepared from ratfish (Hydrolagus colliei) liver oil, were studied for their in vitro effect on human neutrophil migration. The mixture containing unsaturated compounds (II) was more active compared to the saturated (I) ones at a range from 10(-6) M to 10(-10) M concentrations. At 10(-4) M, II was cytotoxic. Both I and II were more potent than synthetic "PAF-acether" (III) and the material prepared from beef heart plasmalogens (IV). Preincubation and checkerboard titration experiments showed that the ether phospholipids derived from ratfish liver oil have primarily chemokinetic, but also chemotactic effects on neutrophils, as has been reported fo compound III. These compounds are therefore highly potent stimulants of human neutrophils with potentially unique membrane-activating properties.
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects; D006801:Humans; D008099:Liver / Q000032:analysis*; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D009821:Oils / Q000032:analysis*; D010713:Phosphatidylcholines / Q000494:pharmacology*; D010972:Platelet Activating Factor; D013329:Structure-Activity Relationship
D008244:Lysophosphatidylcholines; D009821:Oils; D010713:Phosphatidylcholines; D010972:Platelet Activating Factor