[Lactate level in the tissues of animals with a tumor administered oxythiamine and the properties of lactate dehydrogenase].
Content of lactate in blood, liver and kidney tissues, skeletal muscle and in tumor tissue as well as some properties of lactate dehydrogenase (LDH), isolated from liver tissue, were studied in three groups of rats - intact rats, the tumor-bearing animals with sarcoma S-45 and the tumor-bearing rats treated with hydroxythiamin within 22 days. In skeletal muscle on the side of the tumor localization content of lactate was decreased as compared with the opposite side of the body. As shown by analysis of correlation between the content of lactate and the tumor weight and the lactate concentration in the tumor-bearing rat tissues studied, the tumor appears to be responsible for consumption but not for production of lactate. Hydroxythiamin altered distinctly the ratio of lactate content in various tissues and normalized the liver tissue LDH isoenzyme spectrum in tumor-bearing rats; the vitamin decreased 9- and 15-fold the enzyme specific activity in oxidation of lactate in presence of NAD+ and NADP, respectively. After the hydroxythiamin treatment the apparent KM value of the enzyme, isolated from the tumor-bearing rat liver tissue, was increased with pyruvate and decreased with lactate as substrate.
D000818:Animals; D004353:Drug Evaluation, Preclinical; D005260:Female; D007668:Kidney / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D007773:Lactates / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009368:Neoplasm Transplantation; D010119:Oxythiamine / Q000627:therapeutic use*; D051381:Rats; D012513:Sarcoma, Experimental / Q000188:drug therapy / Q000378:metabolism*; D013844:Thiazoles / Q000627:therapeutic use*
D007773:Lactates; D013844:Thiazoles; D010119:Oxythiamine; D007770:L-Lactate Dehydrogenase
Uroven' laktata v tkaniakh zhivotnykh s opukhol'iu pri vvedenii oksitiaminina i nekotorye svoĭstva laktatdegidrogenazy.