Determination of the antioxidant 3-tert.-butyl-4-hydroxy-anisole in rat-plasma using high-resolution gas chromatography-mass spectrometry.
A method is described for the determination of the antioxidant 3-tert.-butyl-4-hydroxy-anisole in rat plasma using high-resolution capillary gas chromatography-mass spectrometry with selective ion monitoring. Following the addition of the isomer 2-tert.-butyl-4-hydroxy-anisole, used as an internal standard, extraction was made with n-hexane and the extract derivatized with heptafluorobutyric anhydride. The gas chromatographic separation was carried out on a SE-52 fused silica capillary column and the derivatized 3-tert.-butyl-4-hydroxyanisole and its isomer detected by recording the intensities of their common fragment ion at m/e 361. The sensitivity of the method allowed the antioxidant to be measured in 0.1-ml rat plasma samples down to a level of 10 ng/ml with a high degree of specificity and accuracy. The method has been applied to a preliminary pharmacokinetic study in rats after oral dosage.
D000818:Animals; D000840:Anisoles / Q000097:blood*; D002083:Butylated Hydroxyanisole / Q000008:administration & dosage / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008297:Male; D051381:Rats
D000840:Anisoles; D002083:Butylated Hydroxyanisole