The effect of hypoxia on the metabolism of labeled glucose and acetate in the rat brain.
Glucose consumption and utilization of amino acids, lipids and proteins was measured in the rat brain under normoxia and hypoxia (7%O2:93%N2). After 2 h of hypoxia the brain glucose consumption increased by over 60% of control value. In spite of this increase, the radioactivity of amino acid fraction did not increase or parallel changes of glucose radioactivity in the blood. This strongly suggested that glucose flux into amino acids remained unchanged in hypoxia. Incorporation of 14C from glucose into macromolecules was found to decrease. The above changes demonstrated that the metabolic steps which precede synthesis of amino and tricarboxylic acids were inhibited. In some experiments, the incorporation of 14C from [2-14C]-acetate into a macromolecular fraction was also measured. The amounts of radioactivity found in these fractions were unchanged under hypoxic conditions. Possible differences in the influence of hypoxia on macromolecular synthesis in different metabolic compartments of the brain are discussed.
D000085:Acetates / Q000097:blood / Q000378:metabolism*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry / Q000528:radiation effects; D002250:Carbon Radioisotopes; D005947:Glucose / Q000378:metabolism*; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D011868:Radioisotopes; D051381:Rats; D011919:Rats, Inbred Strains; D014316:Tritium / Q000378:metabolism
D000085:Acetates; D001786:Blood Glucose; D002250:Carbon Radioisotopes; D011868:Radioisotopes; D014316:Tritium; D005947:Glucose