Changes of blood oxygen affinity in different CPD solutions during liquid storage.
Soft and hard-packed red blood cells in four different CPD anticoagulant-preservative solutions were stored with and without added glucose, adenine, and electrolytes. The hemoglobin-oxygen affinity of the red blood cell concentrates was tested over a six-week storage period. No single solution conferred better protection than any other against an expected increase in oxygen affinity due to loss of 2,3-DPG during storage. In all solutions, P50 at pH 7.4 decreased linearly when measured in a physiological system using CO2. After six weeks' storage at 4 C, the normal oxygen-binding properties of red blood cells could be restored in all instances following incubation for one hour in a rejuvenation solution. By contrast, red blood cell ATP levels were highest when resuspending solutions contained adenine and added glucose, but did not significantly compensate the allosteric role of 2,3-DPG in regulating oxygen affinity when the latter became depleted.
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000925:Anticoagulants / Q000494:pharmacology*; D001793:Blood Preservation; D002951:Citrates / Q000494:pharmacology*; D004163:Diphosphoglyceric Acids / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000097:blood*; D012996:Solutions; D013997:Time Factors
D000925:Anticoagulants; D002951:Citrates; D004163:Diphosphoglyceric Acids; D012996:Solutions; D019794:2,3-Diphosphoglycerate; C007077:citrate phosphate dextrose; D000255:Adenosine Triphosphate; D005947:Glucose; D010100:Oxygen