High-molecular-weight catecholamine--ATP aggregates are absent from the chromaffin-granule aqueous phase.
Direct n.m.r. studies show that the viscous liquid phase which separates from solutions containing physiological concentrations of noradrenaline, ATP and Ca2+ is not present in the chromaffin-granule interior. This finding is verified for both adrenaline and noradrenaline by using 13C n.m.r. spectra.
D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D002118:Calcium / Q000032:analysis; D002417:Cattle; D002837:Chromaffin Granules / Q000032:analysis*; D002838:Chromaffin System / Q000032:analysis*; D004837:Epinephrine / Q000032:analysis; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D009638:Norepinephrine / Q000032:analysis*
D046911:Macromolecular Substances; D000255:Adenosine Triphosphate; D002118:Calcium; D009638:Norepinephrine; D004837:Epinephrine