[Method of applying colchicine to the rat vagus nerve for the purpose of selective action on axonal transport].
A method of local application of colchicine deposited in a wax and paraffin alloy to the nerve is suggested. The doses of 25, 50, 75 and 100 micrograms were tested. Four days after application of 75 and 100 micrograms of colchicine, light microscopy demonstrated a non-uniform thickening of neurofibrils and the increase in their argyrophilic properties. Electron microscopy discovered the reduced number of microtubules, disordered arrangement of neurofilaments and accumulation of the latter ones beneath the neurolemma. The overall electric activity of the nerve was not disturbed. The method of colchicine application in vivo ensures the prolonged action of the drug without nerve compression or injury, thereby enabling one to assess consistently the sequels of the blockade of neurotrophic factors for metabolism of the innervated structures.
D000818:Animals; D001369:Axons / Q000187:drug effects*; D003078:Colchicine / Q000008:administration & dosage*; D008722:Methods; D051381:Rats; D014630:Vagus Nerve / Q000187:drug effects*
D003078:Colchicine
Metod applikatsii kholkhitsina na bluzhdaiushchiĭ nerv krysy s tsel'iu izbiratel'nogo vozdeĭstviia na aksonal'nyĭ transport.