Determination of pentachlorophenol and related compounds in animal materials by high-performance liquid chromatography and gas chromatography.
The rapid determination of pentachlorophenol and tetrachlorophenols in animal materials by high-performance liquid chromatography (HPLC) on porous silica is described. Non-fatty substrates are digested in alkali and the chlorophenols extracted as ion pairs. Fatty materials are extracted with ethyl acetate-hexane after acidification. Extracts are cleaned up on "Sep-Pak" silica or Florisil cartridges. Penta- and tetrachloroanisoles are recovered by the extraction procedures and can be determined by gas chromatography if required. Mean recoveries of the chlorophenols were 73-108% at fortification levels of 0.1-10 mg/kg. A concentration of 0.1 mg/kg can readily be determined and the method can be adapted to reach about 1 microgram/kg. Identities can be confirmed by ion-pair HPLC on a reversed-phase column.
D000818:Animals; D000840:Anisoles / Q000032:analysis; D002733:Chlorophenols / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004531:Eggs / Q000032:analysis; D008099:Liver / Q000032:analysis; D009132:Muscles / Q000032:analysis; D010416:Pentachlorophenol / Q000032:analysis*
D000840:Anisoles; D002733:Chlorophenols; D010416:Pentachlorophenol