Heparin facilitates the extraction of tissue fibronectin.
Extraction of fibronectin from two human tissues, lung parenchyma and placental villi, was facilitated by the incorporation of heparin into extraction media. The effect of heparin was additive to the effect of urea which is known to extract fibronectin. These experiments provide further evidence that fibronectin and glycosaminoglycans are associated in connective tissues and the use of heparin forms the basis for a simple method for extraction and quantitation of tissue fibronectin.
D003871:Dermatan Sulfate; D005260:Female; D005353:Fibronectins / Q000302:isolation & purification*; D006493:Heparin; D006497:Heparan Sulfate; D006801:Humans; D008168:Lung / Q000032:analysis; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D014508:Urea
D003871:Dermatan Sulfate; D005353:Fibronectins; D006493:Heparin; D006497:Heparan Sulfate; D014508:Urea