[Submicroscopic and immunochemical study of horseradish peroxidase passage through the epithelium of the bronchial mucosa].
2% solution of horseradish peroxidase (HRP) was placed on the bronchial mucosa of anaesthetized dogs through a bronchoscope. Arterium femoralis and thracal lymphatic duct were canulated to HRP application. The radiate immunoprecipitation method was utilized to assess HRP activity in blood and lymph samples. In blood, a progressive increase in HRP activity was noted during the experiment, while this activity in lymph rose in 30 minutes, to decrease by the end of the experiment. In parallel series of experiments biopsy specimens were taken from bronchial mucosae exposed to HRP solution. Within all the intervals after HRP administration tested, the product of reaction was clearly demonstrated ultrastructurally within the intracellular spaces and under basal lamina of bronchial epithelium. Pictures suggestive of endocytosis were infrequently encountered in the apical portion of ciliated cells.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001980:Bronchi / Q000378:metabolism* / Q000648:ultrastructure; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004285:Dogs; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D006735:Horseradish Peroxidase / Q000378:metabolism*; D007120:Immunochemistry; D008854:Microscopy, Electron; D009092:Mucous Membrane / Q000378:metabolism / Q000648:ultrastructure; D010544:Peroxidases / Q000378:metabolism*
D006735:Horseradish Peroxidase; D010544:Peroxidases
Submikroskopicheskoe i immunokhimicheskoe izuchenie prokhozhdeniia peroksidazy khrena cherez épiteliĭ slizistoĭ bronkha.