Relationship between in vivo mitomycin C exposure, sister chromatid exchange induction and in vitro mitogenic proliferation. I. Development of murine splenic cell system.
In vivo administration of mitomycin C (MMC) to C57BL/6J mice induced a rapid, initial suppression (24 hours post injection) of in vitro mitogenesis. This was followed by of the mitogen concentration-response curves were found of the three mitogens, phytohemagglutinin and concanavalin A, but not for lipopolysaccharide. By 144 hours post injection, the dose-response curves and magnitude of the responses had returned to approximately control (untreated) levels. This approach provides a model system for the functional assessment of in vivo cellular damage by MMC.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D003434:Crossing Over, Genetic / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008937:Mitomycins / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013154:Spleen / Q000166:cytology*
D008070:Lipopolysaccharides; D008937:Mitomycins; D010835:Phytohemagglutinins; D003208:Concanavalin A