title: In vitro spermatogenesis in Drosophila. I. Development of isolated spermatocyte cysts from wild-type D. hydei.
abstract: In vitro spermatogenesis of isolated single spermatocyte cysts of Drosophila hydei was studied by microscopic observations and time-lapse cinematography. Cysts of spermatocytes isolated during diplotene develop as far as the coiling stage of spermatid differentiation. The existence of an interphase between meiosis I and meiosis II is, for the first time, documented. Meiosis, Nebenkern formation, and elongation of spermatids occur just as in D. melanogaster; however, an individualization cone, as described for D. melanogaster, can not be detected.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000502:physiology; D004330:Drosophila / Q000502:physiology*; D008297:Male; D008540:Meiosis; D009924:Organ Culture Techniques; D013087:Spermatids / Q000166:cytology; D013090:Spermatocytes / Q000166:cytology; D013091:Spermatogenesis