Evidence for the presence of the trimannosyl-di-N-acetylchitobiose core in the carbohydrate chains of human-parotid, proline-rich glycoprotein.
The carbohydrate chains of the human-parotid, proline-rich glycoprotein are linked through a single type of carbohydrate-peptide linkage (asparaginyl-N-acetylglucosamine). The structure of the internal part of the carbohydrate chains, determined by chemical, enzymic, and g.l.c.--m.s. methods, includes the trimannosyl-di-N-acetylchitobiose core involved in the carbohydrate-peptide linkage. Furthermore, an L-fucose residue is linked to the 2-acetamido-2-deoxy-D-glucosyl residue linked to the L-asparaginyl residue. The sequence of the peripheral part of the chains has also been determined as alpha-L-Fuc rho leads to beta-D-Gal rho leads to beta-D-Glc rho NAc leads to alpha-D-Man rho, suggesting a double-branched, basic carbohydrate structure.
D005936:Glucans / Q000032:analysis*; D006020:Glycopeptides / Q000032:analysis; D006023:Glycoproteins; D006801:Humans; D007202:Indicators and Reagents; D008351:Mannans / Q000032:analysis*; D008745:Methylation; D009844:Oligosaccharides / Q000032:analysis; D010306:Parotid Gland / Q000032:analysis*; D011134:Polysaccharides / Q000032:analysis*; D012471:Salivary Proteins and Peptides
D005936:Glucans; D006020:Glycopeptides; D006023:Glycoproteins; D007202:Indicators and Reagents; D008351:Mannans; D009844:Oligosaccharides; D011134:Polysaccharides; D012471:Salivary Proteins and Peptides; C032696:trimannosyl-di-N-acetylchitobiose