Influence of pH and ionic strength on the lysis of Micrococcus luteus cells by hen lysozyme at low (20 degree C) and high (physiological, 40 degree C) temperature.
From isoactivity curves (showing activity as a function of Ph and ionic strength) it was found that in the pH domain 6.7-8.6 frequently used in experiments involving hen lysozyme, the pH optimum of lysis of Micrococcus luteus cells at low ionic strength (0.02-0.05) by the high-temperature form (40 degree C, physiological temperature) was one to two pH units lower than that by the low-temperature form (20 degree C).
D000818:Animals; D002645:Chickens; D006863:Hydrogen-Ion Concentration; D008837:Micrococcus / Q000378:metabolism*; D009113:Muramidase / Q000378:metabolism*; D009994:Osmolar Concentration; D013696:Temperature
D009113:Muramidase