[A rapid method for continuous flow measurement of cholesterol contained in high density lipoproteins (HDL) (author's transl)].
The authors utilized a reagent containing concanavalin A, a vegetal lecithin, to selectively precipitate lipoproteins containing apoprotein B, a component of VLDL, LDL, and Lp (a) which are well known for their atherogenic risk. During this precipitation "true" high density lipoproteins remain in solution. HDL cholesterol determination which constitutes an indirect indication of HDL activity or concentrations is performed by an enzymatic method using an automated continuous flow technique carried out on an Auto Analyzer II (Technicon Corp.). This rapid, easy determination obtains results comparable to other methods, particularly those chosen by the Société Française de Biologie Clinique (French Society of Clinical Biology). This technique should permit all laboratories to confirm an atherogenic index.
D001053:Apolipoproteins / Q000302:isolation & purification; D001055:Apolipoproteins B; D001322:Autoanalysis; D002784:Cholesterol / Q000097:blood*; D008076:Cholesterol, HDL; D003208:Concanavalin A; D006801:Humans; D007202:Indicators and Reagents; D008075:Lipoproteins, HDL / Q000097:blood*
D001053:Apolipoproteins; D001055:Apolipoproteins B; D008076:Cholesterol, HDL; D007202:Indicators and Reagents; D008075:Lipoproteins, HDL; D003208:Concanavalin A; D002784:Cholesterol
Méthode de dosage rapide du cholestérol des lipoprotéines de haute densité (HDL) en flux continu.