[Isolation and physico-chemical properties of P-9 protein, marker for the resting cells of lymphoma NK/Ly and Gerene carcinoma].
A fraction of tumour cells in the state of proliferative rest was prepared by sedimentation from aged Ehrlich tumor and lymphoma NK/Ly. These cells do not incorporate [3H]-thymidine and contain fewer proteins and RNAs than the proliferating cells. The incorporation of [3H]-uridine by these cells makes up to 3.5-10% of the maximal incorporation by proliferating cells at the S-phase. The resting cells have a low rate of glycolysis and a high rate of respiration. The activity of lactate dehydrogenase, glucose-6-phosphate dehydrogenase and malate dehydrogenase in these cells is considerably reduced. The resting cell nuclei were found to contain a low molecular weight protein with molecular weight of 9000 +/- 500 (protein P-9) absent in proliferating cell nuclei. Using acetone fractionation and a subsequent preparative electrophoresis in a phenol solution, protein P-9 was isolated from the nuclei of lymphoma NK/Ly and Gerene carcinoma in a pure state. The physico-chemical properties of the protein from various sources were found to be similar. Thus, protein P-9 can be attributed to low molecular weight non-histone proteins with pI at the weakly alkaline region of pH.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000503:physiopathology*; D002455:Cell Division; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008223:Lymphoma / Q000503:physiopathology*; D008291:Malate Dehydrogenase / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D010101:Oxygen Consumption
D000596:Amino Acids; D009363:Neoplasm Proteins; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase
Vydelenie i fiziko-khimicheskie svoĭstva belka P-9, markiruiushchego pokoiashchiesia kletki limfomy NK/Ly i kartsinomy Gerena.