[Effect of phospholipase A2 from the venoms of bee and Middle Asian cobra on choline uptake by synaptosomes].
The effect of purified phospholipase A2 from venom of the bee Apis mellifica and from venom of the cobra Naja naja oxiana on the Na+-dependent high affinity choline transport into the synaptosomes of rabbit corpus striatum (active uptake) was studied. Both phospholipases A2 were shown to inhibit the active choline uptake by the synaptosomes. The bee venom phospholipase at a concentration of 10(-8) M and the cobra venom phospholipase at a concentration of 10(-6) M produced a 50% inhibition of choline uptake. A relationship was found between the enzymatic activity of the phospholipases and their ability to block choline uptake by the synaptosomes. A removal of Ca-ions from the medium abolished the effects of both phospholipases. Replacement of Ca2+ by Sr2+ inhibited the effect of the cobra venom phospholipases but did not inhibit that of bee venom enzyme.
D000818:Animals; D001514:Bee Venoms / Q000494:pharmacology*; D001693:Biological Transport, Active / Q000187:drug effects; D002794:Choline / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D004546:Elapid Venoms / Q000494:pharmacology*; D007700:Kinetics; D010740:Phospholipases / Q000494:pharmacology*; D010741:Phospholipases A / Q000494:pharmacology*; D054467:Phospholipases A2; D011817:Rabbits; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*
D001514:Bee Venoms; D004546:Elapid Venoms; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2; D002794:Choline
Vliianie fosfolipaz A2 iz iadov pchely i sredneaziatskoĭ kobry na zakhvat kholina sinaptosomami.