[Modification of the method for isolating proteoglycan fractions from cartilage tissue].
A method is suggested for isolating proteoglycans from cartilage tissue with their simultaneous separation into fractions. A successive treatment of cartilage with solutions of different salts and papain produced proteoglycans monomers with short and long chains of glycosaminoglycans, proteoglycans aggregates free and bound with collagen. The content of proteoglycans fractions in the unchanged and degeneratively changed human articular cartilage is determined, the results are given. The data obtained by the method suggested are comparable with the results by other determination methods. The method is technically simple and may be applied for the analysis of the operation and section material.
D002356:Cartilage / Q000032:analysis*; D002358:Cartilage, Articular / Q000032:analysis*; D006801:Humans; D046911:Macromolecular Substances; D008722:Methods; D010206:Papain; D011509:Proteoglycans / Q000302:isolation & purification*
D046911:Macromolecular Substances; D011509:Proteoglycans; D010206:Papain
Modifikatsiia metoda vydeleniia fraktsiĭ proteoglikanov iz tkani khriatsa.