Rejuvenation of aged erythrocytes by incorporating phosphoenolpyruvate into the cells.
An acid-citrate dextrose solution which contains phosphoenolpyruvate and sucrose was used as a preservative and medium for rejuvenating depleted erythrocytes. 2,3-bisphosphoglycerate and ATP in washed erythrocytes, red cell concentrate or whole blood were increased effectively by incubating the cells with the solution at 37 degrees C for 30--60 min. The transport of phosphoenolpyruvate through the erythrocyte membrane was essential to the increase of 2,3-bisphosphoglycerate and ATP. During storage of the cells at 4 degrees C in the presence of phosphoenolpyruvate, no increase of 2,3-bisphosphoglycerate and ATP was observed because no transport of phosphoenolpyruvate into the cells occurred. By an incubation at 37 degrees C for 30 min at the end of storage periods, however, levels of ATP and 2,3-bisphosphoglycerate in the cells were raised.
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000097:blood; D001692:Biological Transport; D001793:Blood Preservation; D004163:Diphosphoglyceric Acids / Q000097:blood; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010728:Phosphoenolpyruvate / Q000378:metabolism / Q000494:pharmacology*
D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate