Cell surface labeling of embryonic neural retina cells exposed to low temperature, energy inhibitors, cytochalasin B and colchicin.
Large blebs devoid of receptors for hemocyanin-labeled concanavalin A appear on neural retina cells from 8-day chick embryos exposed to low temperature or to potassium cyanide or 2,4(alpha)-dinitrophenol at room temperature. Labeling with mixed antibodies against a crude retina membrane preparation and with goat anti-rabbit-hemocyanin conjugate showed the same results. Determination of cell ATP content indicated a drop in ATP concentration after exposure to low temperature or to respiration inhibitors. Disruption of microtubules by colchicin inhibited the formation of 'naked' large blebs, whereas cytochalasin B had no such inhibitory effect.
D019297:2,4-Dinitrophenol; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology*; D003208:Concanavalin A; D003571:Cytochalasin B / Q000494:pharmacology*; D004140:Dinitrophenols / Q000494:pharmacology; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000502:physiology; D011190:Potassium Cyanide / Q000494:pharmacology; D012160:Retina / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure; D014475:Uncoupling Agents / Q000494:pharmacology*
D000954:Antigens, Surface; D004140:Dinitrophenols; D014475:Uncoupling Agents; D003208:Concanavalin A; D003571:Cytochalasin B; D011190:Potassium Cyanide; D019297:2,4-Dinitrophenol; D003078:Colchicine