title: Recovery of secretin in acid small intestinal lumen perfusates in the rabbit.
abstract: Immunoreactive secretin concentrations were measured in femoral venous plasma and in intestinal perfusates during acid and neutral perfusion of small intestinal segments in anesthetized rabbits. Steady-state plasma and luminal secretin concentrations were less than 20 pg/ml. Duodenal perfusion with with 0.1 N hydrochloric acid stimulated peak plasma immunoreactive secretin concentrations of 200 - 300 pg/ml within five minutes. Acid stimulated plasma concentrations remained constant during 30 minutes of perfusion. Secretin output into the acid perfusate (2000 - 4000 pg/min) accompanied the release into plasma. Subsequent perfusion with a neutral solution resulted in a rapid return to steady-state plasma and luminal secretin concentrations. Sequential acid perfusion of the ileum, jejunum, and duodenum stimulated similar concentrations of secretin in plasma and luminal perfusates.
mesh_terms: D000818:Animals; D002766:Cholecystokinin / Q000378:metabolism; D004386:Duodenum / Q000378:metabolism; D006851:Hydrochloric Acid / Q000494:pharmacology*; D007082:Ileum / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000187:drug effects / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D007700:Kinetics; D008297:Male; D010477:Perfusion; D011817:Rabbits; D012633:Secretin / Q000097:blood / Q000378:metabolism*
chemical_list: D012633:Secretin; D002766:Cholecystokinin; D006851:Hydrochloric Acid