title: Carbon-13 nuclear magnetic resonance spectroscopic study of teichuronic acid from Micrococcus luteus cell walls. Comparison of the polysaccharide isolated from cells with that synthesized in vitro.
abstract: Teichuronic acid isolated from the cell walls of Micrococcus luteus has been examined by natural-abundance (13)C NMR spectroscopy. Proton-decoupled and proton-coupled spectra were obtained for native teichuronic acid and also after the teichuronic acid had been oxidized with periodate and reduced with borohydride. The spectra are consistent with the structure [ManNAcUAp-( beta -1,6)-G1cp-( alpha -1,4)]n. Teichuronic acid synthesized in vitro from suitable substrates by the particulate enzyme fraction obtained from M. luteus yielded a (13)C NMR spectrum which is indistinguishable from that of the native teichuronic acid, indicating a structural identity of the teichuronic acid synthesized in vitro with that isolated from cell walls.
mesh_terms: D002473:Cell Wall / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008837:Micrococcus / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000096:biosynthesis; D014574:Uronic Acids / Q000032:analysis* / Q000096:biosynthesis
chemical_list: D011135:Polysaccharides, Bacterial; D014574:Uronic Acids; C010788:teichuronic acid