Rapid and direct staphylocoagulase assay that uses a chromogenic substrate for identification of Staphylococcus aureus.
A rapid and direct assay of staphylocoagulase that uses chromogenic substrate Chromozym TH was developed for the routine identification of Staphylococcus aureus. False-positive and false-negative reactions were eliminated by measuring the activity of staphylocoagulase-prothrombin complex directly. The method was compared with three clotting assays. The results show that the new assay is specific, quantitative, and easily automated. If offers clinical laboratories a more reliable assay of staphylocoagulase and is a valuable aid for the rapid identification of S. aureus.
D001777:Blood Coagulation; D003030:Coagulase / Q000378:metabolism*; D003470:Culture Media; D005189:False Positive Reactions; D008722:Methods; D009842:Oligopeptides / Q000378:metabolism; D011516:Prothrombin / Q000378:metabolism; D012710:Serum Albumin, Bovine; D013211:Staphylococcus aureus / Q000145:classification* / Q000201:enzymology
D003030:Coagulase; D003470:Culture Media; D009842:Oligopeptides; D012710:Serum Albumin, Bovine; C014413:chromozym TH; D011516:Prothrombin