Serial propagation of human endothelial cells in vitro.
Human umbilical vein (HUV) endothelial cells were grown for 15 to 21 passages at a split ratio of 1:5 (at least 27 population doublings) on a human fibronectin (HFN) matrix in Medium 199 supplemented with fetal bovine serum (FBS) and endothelial-cell growth factor (ECGF). This system also permitted the growth of HUV endothelial cells at cell densities as low as 1.25 cells/cm2. In addition to delaying the premature senescence of HUV endothelial cells, ECGF also reduced the serum requirement for low-density HUV endothelial-cell growth; 2.5% serum and ECGF yields half-maximum growth as compared to high serum controls. Significant HUV endothelial-cell growth was also observed in medium supplemented with either ovine hypophysectomized (HYPOX) serum, plasma-derived serum (PDS), or HYPOX-PDS in the presence of ECGF, suggesting that neither the pituitary nor the platelet contributes to HUV endothelial-cell growth.
D000818:Animals; D001769:Blood; D002417:Cattle / Q000097:blood; D002448:Cell Adhesion; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured / Q000166:cytology*; D002999:Clone Cells; D003470:Culture Media; D004727:Endothelium / Q000166:cytology*; D005353:Fibronectins / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D007016:Hypophysectomy; D012756:Sheep / Q000097:blood; D014471:Umbilical Veins / Q000166:cytology*
D003470:Culture Media; D005353:Fibronectins; D006133:Growth Substances