The generation of specific cytotoxic responses by human lymphocytes requires antigen and activities provided by a lymphokine supernatant.
The requirements for the generation of specific cytotoxic responses by human lymphocytes have been investigated using as a model system the response to the ultraviolet light-killed mouse tumour P815. These requirements are antigen (P815UV) and activities provided by a human lymphokine (LK) supernatant. Cells that give rise to non-specific cytotoxicity can be separated on discontinuous Percoll density gradients after a 3-day incubation of peripheral blood leucocytes with the lymphokine supernatant. Cells from which specific cytotoxic responses can subsequently be generated in the presence of P815UV and LK sediment at a higher density.
D000818:Animals; D000941:Antigens; D002469:Cell Separation; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D008811:Mice, Inbred DBA
D000941:Antigens; D008222:Lymphokines