GLC determination of itanoxone in biological fluids.
A sensitive and reliable method for the quantitative determination of itanoxone, 4-[4'-(2-chlorophenyl)phenyl]-4-oxo-2-methylenebutanoic acid, in biological fluids is described. A quantitative ethyl acetate extraction of the plasma samples is followed by reduction and methylation of itanoxone. Quantification is achieved by GLC using electron-capture detection and an internal standard. The minimum concentration of itanoxone detected in plasma is 0.1 microgram/ml. Recovery of the titrated compound added to human plasma averaged 100.9 +/- 2.92% (RSD).
D001713:Biphenyl Compounds / Q000032:analysis*; D001826:Body Fluids / Q000032:analysis*; D002090:Butyrophenones / Q000032:analysis*; D002849:Chromatography, Gas; D006801:Humans; D000960:Hypolipidemic Agents / Q000032:analysis*
D001713:Biphenyl Compounds; D002090:Butyrophenones; D000960:Hypolipidemic Agents; C024140:itanoxone