A novel procedure for the rapid purification of plastocyanin from pea (Pisum sativum) leaves.
Plastocyanin is soluble at high concentrations (greater than 3 M) of (NH4)2SO4 but under these conditions will adsorb tightly to unsubstituted Sepharose beads. This observation was utilized to purify plastocyanin from pea (Pisum sativum) in two chromatographic steps. Sepharose-bound plastocyanin was eluted with low-ionic-strength buffer and subsequently purified to homogeneity by DEAE-cellulose chromatography.
D002847:Chromatography, Agarose; D002848:Chromatography, DEAE-Cellulose; D007887:Fabaceae / Q000032:analysis; D008722:Methods; D010940:Plant Proteins / Q000302:isolation & purification*; D010946:Plants, Medicinal; D010970:Plastocyanin / Q000302:isolation & purification*
D010940:Plant Proteins; D010970:Plastocyanin