Sialidase treatment of rat-erythrocytes: a model for cellular aging?
An in vitro assay system was developed to test the propensity of "old" and sialidase-treated rat erythrocytes to be bound and phagocytosed by rat peritoneal macrophages. Cells considered to be old were phagocytosed to a greater degree than those considered to be young. When erythrocytes were treated with immobilized Vibrio cholerae sialidase, higher amounts of sialic acids had to be removed (25--30%) to induce binding and phagocytosis of the cells, than if the cells had been treated with soluble enzyme (9--15%).
D000818:Animals; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology*; D008954:Models, Biological; D009439:Neuraminidase / Q000494:pharmacology*; D010587:Phagocytosis; D051381:Rats; D014734:Vibrio cholerae / Q000201:enzymology
D009439:Neuraminidase