Differential staining of Y chromosomal lampbrush loops of Drosophila hydei.
The lampbrush loops of the Y chromosome in primary spermatocytes of Drosophila hydei can be stained in a site specific manner employing a modified Giemsa technique. In this communication it is shown that pronase pretreatment changes the staining properties of the various Y loops as well as of the X and autosomes. In addition it is shown that the various chromosomal structures display differences in sensitivity against the action of the enzyme supporting the idea of a site specificity of RNP formation.
D000818:Animals; D001399:Azure Stains; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure; D004330:Drosophila / Q000235:genetics*; D005260:Female; D008297:Male; D011402:Pronase / Q000494:pharmacology*; D012730:Sex Chromosomes / Q000648:ultrastructure*; D013090:Spermatocytes / Q000648:ultrastructure; D014960:X Chromosome / Q000648:ultrastructure; D014998:Y Chromosome / Q000648:ultrastructure*
D001399:Azure Stains; D011402:Pronase