Delayed-type hypersensitivity as revealed on the footpads of mice to azobenzenearsonate-acetyl bovine serum albumin.
A strong delayed-type footpad reaction was established in mice using azobenzenearsonate-acetyl bovine serum albumin (ABA-AcBSA) as an antigen. Male ddY/S mice were sensitized by subcutaneous injection of 100 microliter of Freund's complete adjuvant - saline (1:1) emulsion containing 100 microgram of the antigen and challenged by subcutaneous injection of 2.5 microliter of Freund's incomplete adjuvant - saline (1:1) emulsion with 2.5 microgram antigen in the footpad on the 10th day after the sensitization. The analysis of the system which fulfilled criteria for delayed-type hypersensitivity with regard to kinetics, passive transfer and histology of the footpad reaction, and the effect of dexamethasone, indomethacin and quinacrine on the footpad reaction were described.
D000818:Animals; D001391:Azo Compounds / Q000276:immunology*; D003907:Dexamethasone / Q000494:pharmacology; D006614:Hindlimb; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D009113:Muramidase / Q000276:immunology; D011796:Quinacrine / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000276:immunology; D013154:Spleen / Q000637:transplantation; D010132:p-Azobenzenearsonate / Q000276:immunology*
D001391:Azo Compounds; D012710:Serum Albumin, Bovine; D010132:p-Azobenzenearsonate; D003907:Dexamethasone; D009113:Muramidase; D011796:Quinacrine