Surface potential and surface charge density of the cerebral-cortex synaptic vesicle and stability of vesicle suspension.
Using a microelectrophoresis instrument employing the Lazer-Zee system, the electrophoretic mobility of synaptic vesicles isolated from Guinea-pig brain cortex was measured under conditions. The mobility was found to depend on both pH and ionic concentration of the solution. The surface of the synaptic vesicle was shown to be negatively charged under physiological conditions. The isoelectric point was observed at pH 4.0 in 0.01 M NaCl solution. Effects of divalent cations were examined and reversal of surface charge was observed in 0.1 M CaCl2 solution. Interaction of vesicles was also considered on the basis of the DLVO theory of colloid stability by using calculated values of surface charge density and surface potential of the synaptic vesicle.
D000818:Animals; D002458:Cell Fractionation; D002540:Cerebral Cortex / Q000502:physiology*; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D008433:Mathematics; D008564:Membrane Potentials; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D013499:Surface Properties; D013572:Synaptic Vesicles / Q000502:physiology* / Q000648:ultrastructure