Transglycosylation reactions catalysed by two beta-mannanases.
By using [3H]mannobiose as a labelled acceptor, it was possible to demonstrate transfer reactions catalysed by two beta-mannanases, with mannotetraose and mannopentaose as substrates. The enzyme from Streptomyces transfers one mannose unit from the oligosaccharides, whereas the enzyme from fenugreek (Trigonella foenum-graecum) seeds is able to transfer oligomannose residues.
D008351:Mannans / Q000378:metabolism; D008361:Mannosidases / Q000378:metabolism*; D012639:Seeds / Q000201:enzymology; D013302:Streptomyces / Q000201:enzymology; D013780:Tetroses / Q000378:metabolism; D044902:beta-Mannosidase
D008351:Mannans; D013780:Tetroses; C014647:mannobiose; D008361:Mannosidases; D044902:beta-Mannosidase