The effect of high doses of 6-chloro-6-deoxyglucose on the rat.
Male rats given 6-chloro-6-deoxyglucose (240 mg/kg/day for 28 days) developed spermatocoeles in their ductuli efferentes or caput epididymides. They had a lower serum triglyceride content than controls (0.87 +/- 0.19 vs 1.84 +/- 0.19 mM, Mean +/- SEM; n = 6) and gained less weight (2.55 +/- 0.37 vs 4.1 +/- 0.96 g/day, Mean +/- SEM; n = 6). There was no effect on female rats which received the same treatment. Spermatocoeles could also be produced by a single dose of 6-chloro-6-deoxyglucose, the threshold dose was between 180 and 240 mg/kg. Glucose oxidation by liver, brain, kidney and diaphragm from rats given 6-chloro-6-deoxyglucose (240 mg/kg/day for 14 days) was the same as in controls but was decreased in seminiferous tubules (0.32 +/- 0.06 vs 0.74 +/- 0.02 mumol [U-14C]glucose oxidised to 14CO2/g fresh wt/h, Mean +/- SEM; n = 3). The activity of glyceraldehyde-3-phosphate dehydrogenase [E.C. 1.2.1.12] in liver, brain, testis or muscle from rats given 6-chloro-6-deoxyglucose (24 mg/kg/day for 14 days) showed little change although its activity in spermatozoa was dramatically decreased.
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003272:Contraceptive Agents, Male / Q000494:pharmacology*; D003837:Deoxy Sugars / Q000633:toxicity*; D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000494:pharmacology / Q000633:toxicity*; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005837:Genitalia, Male / Q000187:drug effects / Q000503:physiopathology*; D005947:Glucose / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D008297:Male; D009928:Organ Specificity; D051381:Rats; D012737:Sex Factors; D013088:Spermatocele / Q000139:chemically induced*; D014280:Triglycerides / Q000097:blood
D003272:Contraceptive Agents, Male; D003837:Deoxy Sugars; D014280:Triglycerides; C021163:6-chloro-6-deoxyglucose; D003847:Deoxyglucose; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005634:Fructose-Bisphosphate Aldolase; D005947:Glucose