Establishment and characterization of a cell line (SS78) from a human renal cell carcinoma.
A new cell line, SS78, was established from a primary renal cell carcinoma of a Caucasian male. The tissue was dispersed with collagenase, and viable cells were separated by flotation on a Ficoll-Hypaque gradient. In culture, the SS78 cells retained a distinct epithelial morphology, and no fibroblast like cells were seen. The cultured cells were aneuploid with a modal chromosome number of 80 and had several marker chromosomes. Inoculation of the cultured cells into athymic nude mice caused tumors at the sites of inoculation.
D000782:Aneuploidy; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002871:Chromosome Banding; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D006801:Humans; D007621:Karyotyping; D007680:Kidney Neoplasms / Q000473:pathology; D008297:Male; D051379:Mice; D008875:Middle Aged; D008928:Mitochondria / Q000648:ultrastructure; D009368:Neoplasm Transplantation; D012270:Ribosomes / Q000648:ultrastructure