A new radioimmunoassay for IgM and IgG rheumatoid factors, based on a double antibody method.
A new radioimmunoassay has been developed for measuring IgM and IgG rheumatoid factors. Diluted sera from donors and patients were incubated with immunoprecipitates prepared from sheep serum and rabbit anti-sheep IgG antiserum. The precipitates were washed, and radioiodinated rabbit F(ab')2 fragments specific from human IgM or IgG were added. The precipitates were isolated from filtration and measured in a gamma counter. With this assay IgM rheumatoid factors were detected in sera from all patients with seropositive rheumatoid arthritis and in sera from 40% of patients with seronegative rheumatoid arthritis. IgG rheumatoid factors were found in sera from 68% of the seropositive and 40% of the seronegative patients. Gel filtration experiments demonstrated that it is possible to detect monomeric IgG rheumatoid factors and IgM rheumatoid factors of molecular weight smaller than pentameric IgM. Furthermore it has been shown that IgG rheumatoid factor activity is still present after reduction of IgM rheumatoid factors with dithiothreitol.
D000818:Animals; D000918:Antibody Specificity; D001168:Arthritis / Q000150:complications / Q000175:diagnosis / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000175:diagnosis / Q000276:immunology; D004229:Dithiothreitol / Q000494:pharmacology; D005258:Felty Syndrome / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011565:Psoriasis / Q000150:complications / Q000175:diagnosis / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay; D012217:Rheumatoid Factor; D012756:Sheep; D013585:Synovitis / Q000175:diagnosis / Q000276:immunology; D014657:Vasculitis / Q000175:diagnosis / Q000276:immunology
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012217:Rheumatoid Factor; D004229:Dithiothreitol