Use of the microanalytical system KAPA for serial determinations of triglycerides, total cholesterol and HDL cholesterol in capillary plasma.
Enzymatic methods adapted to the KAPA system can be carried out in a final test volume of less than 10 mu 1. The procedure is mechanized, including the pre-analytical steps. Day-to-day precision is 2-6% C.V. for single determinations. Advantages of the technique are: (1) capillary plasma can be used; (2) reagent waste is reduced by about two orders of magnitude compared to common procedures, and (3) 400 specimens can be assayed by one technician per day. These conditions facilitate serial determinations, large scale screening, or repetitive monitoring of values in a group of individuals.
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D001322:Autoanalysis / Q000295:instrumentation / Q000379:methods*; D002196:Capillaries; D002648:Child; D002675:Child, Preschool; D002784:Cholesterol / Q000097:blood*; D008076:Cholesterol, HDL; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D008832:Microchemistry; D012737:Sex Factors; D014280:Triglycerides / Q000097:blood*
D008076:Cholesterol, HDL; D008075:Lipoproteins, HDL; D014280:Triglycerides; D002784:Cholesterol