Studies of pyruvate-water isotope exchange catalysed by erythrocytes and proteins.
Erythrocyte suspensions in buffer made with 2H2O catalyse the exchange of pyruvate protons. This process can be easly observed by spin-echo proton magnetic resonance. The dominant exchange process is shown to be due to the formation of Schiff-base links between pyruvate and amino groups of haemoglobin. Other proteins with free alpha-amino groups also catalyse the exchange. The pH*-dependence of the exchange rate due to hen-egg-white-lysozyme reflects the dissociation of the alpha-amino group.
D000596:Amino Acids / Q000378:metabolism; D002384:Catalysis; D003903:Deuterium / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009113:Muramidase / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014867:Water / Q000378:metabolism*
D000596:Amino Acids; D006454:Hemoglobins; D011506:Proteins; D011773:Pyruvates; D014867:Water; D003903:Deuterium; D009113:Muramidase