The effect of diflunisal on hyaluronic acid production by 'activated' human synovial fibroblasts.
1 Cultured human synovial fibroblasts were 'activated' by leucocytes or poly (I) . poly (C) to overproduce hyaluronic acid. 2 Diflunisal in concentrations of 30, 15 and 10 microgram/ml completely abrogated the overproduction of hyaluronic acid induced by leucocytes. 3 Diflunisal (10 microgram/ml), indomethacin (2 microgram/ml) and aspirin (200 microgram/ml) partially inhibited the overproduction of hyaluronic acid induced by poly (I) . poly (C), although none of them decreased it to control non 'activated' levels. 4 Diflunisal 25 microgram/ml completely abrogated hyaluronic acid overproduction induced by poly (I) . poly (C). 5 This model could be an in vitro indicator for in vivo pharmacological levels of anti-inflammatory drugs.
D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002478:Cells, Cultured; D004061:Diflunisal / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D007962:Leukocytes / Q000502:physiology; D011070:Poly I-C / Q000494:pharmacology; D012459:Salicylates / Q000494:pharmacology*; D013583:Synovial Membrane / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D012459:Salicylates; D004061:Diflunisal; D006820:Hyaluronic Acid; D011070:Poly I-C