Determination of serotonin, its precursors, metabolites and [3H] serotonin in lung by high-performance liquid chromatography with fluorescence detection.
A rapid, simple, sensitive method for the determination of serotonin, its precursors, metabolites and [3H] serotonin in lung is described. Tissue preparation requires only homogenization in dilute perchloric acid and centrifugation prior to separation by high performance liquid chromatography using a reversed-phase column. Detection is based on the native fluorescence of indole compounds and detection limits ranged between 30-90 pg injected. The method has been used to determine these compounds in mouse lung and plasma.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008168:Lung / Q000378:metabolism*; D008297:Male; D051379:Mice; D012701:Serotonin / Q000097:blood / Q000378:metabolism*
D012701:Serotonin