title: Only a small fraction of avian erythrocyte histone is involved in ongoing acetylation.
abstract: We have studied histone acetylation in chicken erythrocytes. We find that about 30% of the histone in these cells is acetylated, however the majority of these histones are not in a dynamic steady state typical of other chicken cells and of mammalian cells, but rather are frozen in this state of modification. A very small fraction of erythrocyte histones are being modified normally but cannot be detected as shifting to higher levels of acetylation upon treatment with butyrate because the amount of histone so modified is small. Nonetheless, chicken erythrocytes incorporate 3H-acetate into histones about 40% as well as seen in the dynamically active HTC cells. This is most likely due to the formation of very high specific activity Acetyl CoA pools in erythrocytes which have very low levels of coenzyme A. We conclude that these genetically inactive cells are involved in only a minor way with histone acetylation.
mesh_terms: D000085:Acetates / Q000097:blood; D000105:Acetyl Coenzyme A / Q000097:blood; D000107:Acetylation; D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002645:Chickens; D003065:Coenzyme A / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism*; D006657:Histones / Q000097:blood*; D051381:Rats
chemical_list: D000085:Acetates; D002087:Butyrates; D006657:Histones; D020148:Butyric Acid; D000105:Acetyl Coenzyme A; D003065:Coenzyme A