The heterogeneous distribution of beta-glucuronidase in purified Golgi membranes.
An improved procedure for the preparation of purified Golgi membranes by upward flotation is described. The mode of binding of membrane fragments to an affinity adsorbent specific for beta-glucuronidase shows that this enzyme has a heterogeneous distribution in Golgi membranes.
D000818:Animals; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D005966:Glucuronidase / Q000378:metabolism*; D006056:Golgi Apparatus / Q000201:enzymology* / Q000648:ultrastructure; D006867:Hydrolases / Q000378:metabolism; D007425:Intracellular Membranes / Q000201:enzymology* / Q000648:ultrastructure; D008099:Liver / Q000201:enzymology
D006867:Hydrolases; D005966:Glucuronidase