Cadaverine in the rat brain: regional distribution and acylation of [14C]cadaverine in vivo and uptake in vitro.
The regional distribution and acylation of intraventricularly injected [14C]cadaverine was studied in the rat brain over a 48-h period. The concentrations of labeled cadaverine and its acyl derivatives, N-monoacetylcadaverine and N-monopropionylcadaverine, were determined in the telencephalon, striatum, hypothalamus, midbrain, cerebellum, and medulla-pons by TLC of their 5-dimethylamino-1-naphthalenesulfonyl derivatives, followed by liquid scintillation spectrometry. The apparent passage of radioactivity from the ventricular space into brain tissue was slow, with the concentrations reaching a peak at 24 h after injection. The percentage of radioactivity in the acyl forms of cadaverine, however, was maximal 4 h after injection, with the propionyl form predominating. The telencephalon, striatum, and hypothalamus contained the highest concentrations of radioactivity, in all three forms, at all elapsed times. A high-affinity uptake mechanism for cadaverine was demonstrated in slices of these tissues. This process was completely inhibited by equimolar concentrations of unlabeled putrescine.
D000215:Acylation; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002103:Cadaverine / Q000378:metabolism*; D002250:Carbon Radioisotopes; D003342:Corpus Striatum / Q000378:metabolism; D003959:Diamines / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009928:Organ Specificity; D051381:Rats; D013687:Telencephalon / Q000378:metabolism
D002250:Carbon Radioisotopes; D003959:Diamines; D002103:Cadaverine