title: Effects of physiol and chemical factors on the viability of Mycobacterium paratuberculosis.
abstract: Alternative methods of storing bovine fecal specimens for delayed culture of Mycobacterium paratuberculosis was studied. Significant (P less than 0.05) losses of viability were sustained by storage at 23 degrees C for 10 days and by treatment with 0.03 Zephiran for 4 and 10 days. Losses were moderate after storage at -70 degrees C for 15 weeks but significant (P greater than 0.05) after storage at 23 degrees C for 4 days. The alternative methods were compared with the standard method of processing specimens upon receipt and after 2 days in transit. Fecal specimens from 18 cattle were cultured.
mesh_terms: D000818:Animals; D001548:Benzalkonium Compounds; D002417:Cattle; D005243:Feces / Q000382:microbiology*; D005615:Freezing; D009161:Mycobacterium / Q000254:growth & development*; D011309:Preservation, Biological / Q000662:veterinary*; D013048:Specimen Handling / Q000662:veterinary*; D013696:Temperature; D013997:Time Factors
chemical_list: D001548:Benzalkonium Compounds