title: Histone gene number in relation to C-value in amphibians.
abstract: We have compared the number of copies of sequences complementary to a cloned Xenopus histone H4 coding sequence in the genomes of Xenopus, Triturus and Ambystoma, amphibian species with widely different C-values (3, 23 and 38pg DNA/haploid genome respectively). Quantitative autoradiography indicates that H4 sequence constitute a greater proportion of the genome the larger that genome is. Measurement of the absolute copy-number by reassociation kinetic analysis indicated 47 +/- 10, 636 +/- 21 2685 +/- 349 copies per haploid genome each in Xenopus, Triturus and Ambystoma respectively. Whilst this confirms a trend of increasing copy-number with increasing C-value, the two are not directly proportional and some other factors must contribute to determining the number of copies of these genes.
mesh_terms: D000557:Ambystoma; D000818:Animals; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000378:metabolism; D005796:Genes; D006657:Histones / Q000235:genetics*; D009695:Nucleic Acid Renaturation; D013045:Species Specificity; D014319:Triturus; D014981:Xenopus
chemical_list: D004274:DNA, Recombinant; D006657:Histones