Red blood cells coated with tumor cell extracts as targets in 51Cr release assays.
Sheep red-blood cells were coated with tumor cell antigens prepared by extractions with 3M KCl. The coated cells were labelled with 51Cr and used as targets in immuno-assays. The technique was found suitable for complement-mediated as well as antibody-dependent cell-mediated reactions, and it offers several applications for use in tumor immunology.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D000951:Antigens, Neoplasm / Q000276:immunology*; D002860:Chromium Radioisotopes; D003165:Complement System Proteins / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D003602:Cytotoxicity, Immunologic; D004912:Erythrocytes; D012756:Sheep / Q000097:blood
D000951:Antigens, Neoplasm; D002860:Chromium Radioisotopes; D003165:Complement System Proteins