Z-DNA conformation of N-2-acetylaminofluorene modified poly(dG-dC).poly(dG-dC) determined by reactivity with anti cytidine antibodies and minimized potential energy calculations.
The conformation of poly(dG-dC).poly(dG-dC), poly(dG).poly(dC), and calf thymus DNA modified with N-acetoxy-N-2-acetylaminofluorene (N-acetoxy-AAF) was examined by extent of reaction with anti cytidine antibodies. In contrast to modified poly(dG).poly(dC0 and DNA, modified poly(dG-dC).poly (dG-dC) failed to react with the antibodies indicating that the base pairing in this polymer is intact. This in consistent with induction of the Z-DNA conformation in AAF modified poly(dG-dC).poly(dG-dC). Using minimized potential energy calculations on the dCpdG-AAF dimer as a model for the modified polymer, it is shown that the proposed Z-DNA conformation is energetically stable. A model is proposed for an AAF modified tetramer, dGpdCpdGpdC, in which the AAF is external to the Z-DNA duplex.
D015073:2-Acetylaminofluorene; D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D002417:Cattle; D003562:Cytidine / Q000276:immunology; D004247:DNA; D009690:Nucleic Acid Conformation; D011089:Polydeoxyribonucleotides; D011863:Radioimmunoassay; D013950:Thymus Gland
D000906:Antibodies; D000936:Antigen-Antibody Complex; D011089:Polydeoxyribonucleotides; C016681:poly(dG).poly(dC); D003562:Cytidine; D004247:DNA; D015073:2-Acetylaminofluorene