title: Solvolysis of chenodeoxycholic acid sulfates.
abstract: Chemical solvolysis of chenodeoxycholic acid sulfates was studied using 4 published methods. Quantitative recovery of chenodeoxycholic acid from the 3-sulfate was obtained with each method. However, only 2 methods yielded chenodeoxycholic acid after solvolysis of the 7-sulfate. In each instance a compound resembling lithocholic acid by GLC but identifiable as a derivative of chenodeoxycholic acid by mass spectrometry was obtained and represents a product formed during solvolysis. Failure to obtain adequate solvolysis of chenodeoxycholic acid 7-sulfate can lead to false identification of monohydroxy bile acids and apparent absence of th 7-sulfate and disulfate esters.
mesh_terms: D002635:Chenodeoxycholic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry
chemical_list: D002635:Chenodeoxycholic Acid; C027616:chenodeoxycholate sulfate conjugate