[Effect of actinomycin D on hematopoietic stem cells].
The effect of actinomycin D on stem hemopoietic cells in the bone marrow of adult mice has been studied by means of spleen colonies. The antibiotic at a dose of 0.4 mu g/ml (g) inhibited the colony formation when applied in vitro and stimulated this process in vivo. The reaction of stem hemopoietic cells to the antibiotic at a dose 0.04 mu g/ml (g) varied: in most experiments no cytotoxic effect was observed both in vitro and in vivo; moreover, in some cases the colony formation increased 1.5--2 times. Possible causes of the stimulating and inhibiting effect of the antibiotic are discussed.
D000818:Animals; D003114:Colony-Forming Units Assay; D003609:Dactinomycin / Q000494:pharmacology*; D004347:Drug Interactions; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006824:Hybridization, Genetic; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D014158:Transcription, Genetic / Q000187:drug effects; D014529:Uridine / Q000494:pharmacology
D003609:Dactinomycin; D014529:Uridine
Deĭstvie aktinomitsina D na stvolovye krovetvornye kletki.