Specimen handling for the assay of adenylates and glycerate 2, 3-bisphosphate in erythrocytes.
ATP, ADP, AMP and glycerate 2, 3-bisphosphate in blood are frequently analyzed in order to evaluate erythrocytes for blood donation and to diagnose hemolytic anemias. In order to find an uncomplicated and safe procedure for the preanalytical treatment of blood a freezing-storing procedure using dry ice was worked out and combined with a later PCA precipitation. The pre-freezing stability of the components was also investigated and found to be best at room temperature.
D019794:2,3-Diphosphoglycerate; D000227:Adenine Nucleotides / Q000097:blood*; D000244:Adenosine Diphosphate / Q000097:blood; D000249:Adenosine Monophosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004355:Drug Stability; D005615:Freezing; D006801:Humans; D007700:Kinetics; D013048:Specimen Handling / Q000379:methods*; D013696:Temperature
D000227:Adenine Nucleotides; D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate