Lack of antibody in rheumatoid sera to autologous synovial macrophages and dendritic cells by antibody-dependent cellular cytotoxicity assays (ADCC).
We tested the hypothesis that antigens which stimulate the chronic immune reactivity in rheumatoid synovia might be associated with synovial macrophages and dendritic cells. ADCC assays were used to test sera from 15 RA patients and 8 non-RA controls for antibody to autologous primary and secondary cell cultures prepared by enzymatic digestion of synovial tissue. Such autoantibody was not detected in any of the 23 patients using assay conditions which minimized potential ADCC blocking effects of immune complexes and multinuclear giant cells. Collagenase producing dendritic and multinuclear giant cells. Collagenase activity and dendritic cells occurred with equal frequency in both RA and non-RA primary monolayers.
D000906:Antibodies / Q000032:analysis*; D000920:Antibody-Dependent Cell Cytotoxicity; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D003712:Dendrites / Q000276:immunology*; D006801:Humans; D008264:Macrophages / Q000276:immunology*; D010003:Osteoarthritis / Q000276:immunology; D013583:Synovial Membrane / Q000166:cytology / Q000276:immunology*
D000906:Antibodies