Cellular origin of cathepsin B in carrageenin-induced granuloma tissues in rats.
Cathepsin B isolated from carrageenin-induced granuloma tissue was separated into two peaks (one large and one small) on CM-cellulose column chromatography. The cathepsin B activities from the peritoneal macrophages and polymorphonuclear leukocytes were eluted at the positions identical to those of the larger (P-D and the smaller (P-II) peaks, respectively. Similarly, the cathepsin B activities in the P-I and P-II fractions from granuloma tissue corresponded to those from macrophages and leukocytes, respectively, in isoelectric focusing in polyacrylamide gel. Both the cathepsin B activities were completely inhibited by iodoacetamide and leupeptin, but phenylmethanesulfonyl fluoride and o-phenanthroline were ineffective. The molecular weight of the cathepsin B of the cellular origin and granuloma tissue was approximately 24,000 by gel filtration. The pI value of cathepsin B in the P-I and P-II fractions from granuloma tissue was 5.09 and 5.18, respectively. These results strongly suggest that two types of cathepsin B in granuloma tissue may be different in cellular origin and may be isozymes.
D000818:Animals; D002351:Carrageenan / Q000009:adverse effects*; D002403:Cathepsins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D003240:Connective Tissue Diseases / Q000139:chemically induced* / Q000503:physiopathology; D006099:Granuloma / Q000139:chemically induced* / Q000503:physiopathology; D007460:Iodoacetamide / Q000494:pharmacology; D007962:Leukocytes / Q000032:analysis; D007976:Leupeptins / Q000494:pharmacology; D008264:Macrophages / Q000032:analysis; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains
D007976:Leupeptins; D002351:Carrageenan; D002403:Cathepsins; D007460:Iodoacetamide