Similarity of biotin-binding activity and immunoreactivity in chicken oviduct and non-oviduct avidin.
Biotin-binding and immunological methods were employed to demonstrate the similarity of oviduct and non-oviduct avidin in the chicken. Oviduct avidin was induced after oestrogen pretreatment by progesterone and non-oviduct avidin by intestinal tissue injury or by intraperitoneal actinomycin D administration. Avidin in the intestine, lung, bursa of Fabricius, plasma, pectoral muscle and liver after injury had biotin-binding activity similar to that of progesterone-induced oviduct avidin: (1) a temperature of 79-83 degree C was required for 50% of the maximum [14C]biotin uptake, (2) maximal exchange occurred only at 90 or 100 degree C and (3) denaturation of protein, i.e., loss of biotin-binding activity, was not yet observed at 100 degree C. Avidin in the intestine, lung, bursa of Fabricius, plasma and pectoral muscle also showed an identical cross-reaction with oviduct avidin. Furthermore, the increase in avidin-like biotin binding in the oviduct and most non-oviduct tissues was significantly correlated with the increase in avidin-like antigen in the tissue. This indicates that avidin induced in chicken non-oviduct tissues by injury or inflammation caused by actinomycin D administration is similar to progesterone-dependent oviduct avidin.
D000818:Animals; D001360:Avidin / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D002645:Chickens; D003609:Dactinomycin / Q000494:pharmacology; D005260:Female; D006358:Hot Temperature; D007422:Intestines / Q000502:physiology; D010047:Ovalbumin / Q000031:analogs & derivatives*; D010057:Oviducts / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology; D011863:Radioimmunoassay; D014018:Tissue Distribution
D002352:Carrier Proteins; C017071:biotin-binding proteins; D001360:Avidin; D003609:Dactinomycin; D011374:Progesterone; D001710:Biotin; D010047:Ovalbumin