Rapid automated determination of D-penicillamine in plasma and urine by ion-exchange high-performance liquid chromatography with electrochemical detection using a gold electrode.
A high-performance liquid chromatographic method for the determination of D-penicillamine in plasma and urine has been developed, based on separation on a cation-exchange resin followed by an amperometric detection of the SH group of D-penicillamine oxidized on a gold electrode. The method has been automated and separations from plasma and urine take 7 and 9 min, respectively. The detection limits are 0.05 micrograms/ml in plasma and 0.2 micrograms/ml in urine, with a coefficient of variation of 2.9% (n = 10).
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange; D006801:Humans; D010396:Penicillamine / Q000097:blood* / Q000652:urine
D010396:Penicillamine