Studies on the structures of the carbohydrate moiety of human prothrombin.
Human prothrombin contains three asparagine-linked sugar chains in one molecule. The sugar chains were quantitatively liberated as radioactive oligosaccharides from the polypeptide moiety by hydrazinolysis followed by N-acetylation and Nab3H4 reduction. All of the oligosaccharides contain N-acetylneuraminic acid. The neutral oligosaccharides obtained from all acidic oligosaccharides by sialidase digestion are identical. By the combination of sequential exoglycosidase digestion and methylation analysis, the structures of the asparagine-linked sugar chains of human prothrombin were confirmed to be as follows: (sequence in text).
D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D004590:Electrophoresis, Paper; D006026:Glycoside Hydrolases / Q000378:metabolism; D006801:Humans; D008745:Methylation; D011516:Prothrombin / Q000032:analysis*
D011516:Prothrombin; D006026:Glycoside Hydrolases