Effect of glucose on carbohydrate synthesis from alanine or lactate in hepatocytes from starved rats.
In hepatocytes from starved rats, 10mM-glucose suppressed in incorporation of 2mM labelled alanine into glucose+glycogen by more than 40%, whereas no inhibition was observed with labelled lactate as substrate. Addition of glycerol instead of glucose did not show this inhibition. The inhibitory effect could also be demonstrated in label-free experiments.
D000409:Alanine / Q000378:metabolism*; D000818:Animals; D002241:Carbohydrates / Q000096:biosynthesis*; D003864:Depression, Chemical; D005947:Glucose / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D013217:Starvation / Q000378:metabolism
D002241:Carbohydrates; D007773:Lactates; D005947:Glucose; D000409:Alanine; D005990:Glycerol