Inhibitory effects of 3'-modified UTP analogues on DNA-dependent RNA polymerase I and II purified from cherry salmon (Onchorhynchus masou) liver.
Various 1-beta-D-xylofuranosyl-5-substituted uracil 5'-triphosphates were synthesized and their inhibitory effects on DNA-dependent RNA polymerase I and II, which were purified from cherry salmon (Onchorhynchus masou) liver, were examined. The results were as follows: 1) Xylo UTP and xylo TTP were strongly inhibited for both RNA polymerase I and II. 2) 5-Ethyl, 5-n-propyl and 5-n-butyl derivatives showed little inhibitory effect on RNA polymerase I while RNA polymerase II activity was strongly affected by these derivatives. 3) Four kinds of 5-halogenated derivatives including fluoro, chloro, bromo and iodo substituent inhibited both RNA polymerase I and II in almost same extent. 4) The mode of inhibition was, in all cases, competitive with UTP.
D000818:Animals; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D012318:RNA Polymerase I / Q000037:antagonists & inhibitors*; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors*; D012474:Salmon; D013329:Structure-Activity Relationship; D014500:Uracil Nucleotides; D014544:Uridine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology
D014500:Uracil Nucleotides; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I; D014544:Uridine Triphosphate