Clinical evaluation of two fast methods for creatine kinase isoenzyme assay.
Sixty patients admitted to the Cardiac Care Unit were evaluated serially for the presence of CK-MB by electrophoresis and by two comparative fast methods each involving a minimal amount of procedural steps. Immunoinhibition was run manually and by centrifugal analysis. Batch ion-exchange was run in an automated fashion. Correlation among the three methods was good. Further, all three methods displayed excellent accuracy with diagnostic efficiencies ranging from 90% (batch ion-exchange) to 95% (electrophoresis). Utilization of the immunoinhibition reagent on a centrifugal analyzer gave high throughput and reliable results.
D002318:Cardiovascular Diseases / Q000201:enzymology; D002852:Chromatography, Ion Exchange / Q000379:methods; D003402:Creatine Kinase / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007527:Isoenzymes
D007527:Isoenzymes; D003402:Creatine Kinase