Regeneration of 2,3-bisphosphoglycerate and ATP of stored erythrocytes by phosphoenolpyruvate; a new preservative for blood storage.
In ACD solution which contains PEP and sucrose was used as a preservative solution as well as a medium for rejuvenating depleted erythrocytes. 2,3-DPG and ATP in whole blood or red cell concentrates were increased effectively by incubating the cells with the solution at 37 degrees C for 30-60 min. The transport of PEP through the erythrocyte membrane was essential to the increase of 2,3-DPG and ATP. During storage of the cells at 4 degrees C in the presence of PEP, no increase of 2,3-DPG and ATP was observed because no transport of PEP into the cells occurred. By incubation at 37 degrees C for 30 min at the end of various storage periods, however, the levels of ATP and 2,3-DPG in the cells were raised. Addition of ascorbic acid, purine or purine nucleosides to the ACD-sucrose-PEP solution improved the PEP effect of maintaining ATP and/or 2,3-DPG in erythrocytes during storage at 4 degrees C.
D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate / Q000097:blood*; D001800:Blood Specimen Collection; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D007700:Kinetics; D010728:Phosphoenolpyruvate / Q000494:pharmacology*
D004163:Diphosphoglyceric Acids; D019794:2,3-Diphosphoglycerate; D010728:Phosphoenolpyruvate; D000255:Adenosine Triphosphate