Radioimmunoassay of glipizide in human plasma.
A simple, sensitive radioimmunoassay has been developed for the direct determination of glipizide in human plasma. Antisera raised in rabbits immunized with a glipizide analogue conjugated to bovine serum albumin were highly specific, the two main metabolites, 3, cis-hydroxycyclohexyl derivative and 4,trans-hydroxycyclohexyl derivative, having cross reactivities of 0.73% and 1.66%, respectively. The method can measure amounts as small as 1 ng/ml. The intra- and inter-assay coefficients of variation lay between 2.98-5.79% and 2.35-8.66%, respectively. The mean recovery of glipizide added to plasma was 99-105% over the range 1-500 ng/ml. The method was employed to determine plasma levels in six subjects after administration of a 5 mg tablet of glipizide. The results were in accordance with those found after administration of the same dose of radiolabelled glipizide to two other subjects.
D000284:Administration, Oral; D000818:Animals; D003919:Diabetes Insipidus / Q000188:drug therapy; D005913:Glipizide / Q000008:administration & dosage / Q000097:blood* / Q000627:therapeutic use; D006801:Humans; D007106:Immune Sera; D008297:Male; D011817:Rabbits; D011863:Radioimmunoassay; D013453:Sulfonylurea Compounds / Q000097:blood*
D007106:Immune Sera; D013453:Sulfonylurea Compounds; D005913:Glipizide