In vitro formation of hybrid toxins between subunits of Escherichia coli heat-labile enterotoxin and those of cholera enterotoxin.
Heat-labile enterotoxin (LT) was purified from cells of enterotoxigenic Escherichia coli isolated from a patient with traveller's diarrhea. Purified LT was separated into A and B subunits by treatment with 6 M urea solution in 0.1 M propionic acid (pH 4.0). Biologically active toxin was reconstituted from isolated A and B subunits of LT. Hybrid toxins with biological activity were obtained in vitro from the A subunit of cholera enterotoxin and B subunit of LT, and from the A subunit of LT and B subunit of cholera enterotoxin. The hybrid toxins show a similar toxicity to that of the parent toxins from which the A subunits were derived. The in vitro formations of the hybrid toxins were confirmed by polyacrylamide gel disk electrophoresis.
D000818:Animals; D001427:Bacterial Toxins; D001681:Biological Assay; D055598:Chemical Phenomena; D002621:Chemistry; D002772:Cholera Toxin / Q000302:isolation & purification / Q000633:toxicity; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000302:isolation & purification / Q000633:toxicity; D029968:Escherichia coli Proteins; D011817:Rabbits
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli