Effect of pregnant mare serum gonadotropin and estradiol on the formation of 13,14-dihydroprostaglandin F2 alpha in rat ovary.
Effects of pregnant mare serum gonadotropin (PMS) and estradiol on the formation of 13,14-dihydroprostaglandin F2 alpha (13,14H2-PGF2 alpha) from prostaglandin F2 alpha (PGF2 alpha) and 13,14-dihydro-15-ketoprostaglandin F2 alpha (15KD-PGF2 alpha) in the ovarian homogenate of rats were examined. PMS and estradiol were given to the rats (s.c.) on the first day of diestrus, and the ovaries were removed 24 hours after treatment with each hormone. PMS stimulated the formation of 13,14H2-PGF2 alpha from PGF2 alpha and 15KD-PGF2 alpha, and estradiol markedly inhibited the formation of 13,14H2-PGF2 alpha from PGF2 alpha and 15KD-PGF2 alpha. However, the formation of 15KD-PGF2 alpha from PGF2 alpha did not change with PMS and estradiol. These results indicate that 13,14H2-PGF2 alpha formed in rat ovary may play some important role in ovarian function and may be regulated by gonadotropins and ovarian steroids.
D000818:Animals; D015237:Dinoprost / Q000031:analogs & derivatives*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D066298:In Vitro Techniques; D010053:Ovary / Q000187:drug effects*; D011460:Prostaglandins F / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats
D006064:Gonadotropins, Equine; D011460:Prostaglandins F; C026527:13,14-dihydroprostaglandin F2alpha; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; D004958:Estradiol; D015237:Dinoprost