[Luminescence study of the spatial organization of protein-lipid complexes of mitochondrial membranes in different functional states].
Molecular organization of protein-lipid complexes of membranes in different functional states of mitochondria was studied from luminescence of pyrene and pyrene-butyrate which quenched the luminescence of mitochondrial membrane proteins. Maximum quenching was about 30 per cent. This value is found to be constant for a given type of membranes. Mitochondrial membrane proteins are organized into bulk assemblies hardly accessible for quenchers. Essential conformational changes in protein assemblies on functioning of the respiratory chain were not revealed. Changes in epimerization of pyrene in different states of the mitochondria may point to changes in viscosity of hydrophobic regions of membranes.
D000818:Animals; D007425:Intracellular Membranes; D008163:Luminescent Measurements; D008563:Membrane Lipids; D008565:Membrane Proteins; D008930:Mitochondria, Liver / Q000502:physiology*; D011721:Pyrenes / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence
D008563:Membrane Lipids; D008565:Membrane Proteins; D011721:Pyrenes; C002218:1-pyrenebutyrate
Liuminestsentnoe izuchenie prostranstvennoĭ organizatsii belok-lipidnykh kompleksov mitokhondrial'nykh membran v razlichnykh funktsional'nykh sostoianiiakh.