title: [Phosphorylation reactions of lysyl-tRNA-synthetase from rat liver].
abstract: The phosphorylation reaction of lysyl-tRNA-synthetase from the rat liver tissue was studied in the in vivo experiments. The activity of 3':5'-AMP-dependent protein kinase from the myocardium in the phosphorylation reaction is 3.4 times lower than that of the enzyme from the liver. Optimum conditions of the lysyl-tRNA-synthetase phosphorylation reaction were determined. The Vmax and Km values for the reaction were 2.7 pmol of 32P/mg per 1 minute and 0.4-10(-4), respectively. The lysyl-tRNA-synthetase phosphorylation increases the tRNA aminoacylation.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008250:Lysine-tRNA Ligase / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats
chemical_list: D011494:Protein Kinases; D000604:Amino Acyl-tRNA Synthetases; D008250:Lysine-tRNA Ligase
vernacular_title: Izuchenie reaktsii fosforilirovaniia lizil-tRNK-sintetazy iz pecheni krys.