Monoclonal anti-azobenzenearsonate antibodies expressing the cross-reactive idiotype: immunochemical studies show that all idiotypic determinants reside on a single molecule.
Cell lines that secreted antibodies to the hapten azobenzenearsonate (ABA) were established by hybridization of immune A/J spleen cells to the non-secreting myeloma, NS-1. Solid-phase radioimmunoassays (RIA) were developed for rapid screening of hybridoma supernatants to detect antibodies to ABA and to detect antibodies bearing the ABA cross-reactive idiotype (CRI). Hybrid clones secreting both CRI+ and CRI-- anti-ABA antibodies were obtained. The supernatant from one clone (7-13) strongly inhibited binding of iodinated anti-idiotype serum in a competitive RIA. This clone expressing the CRI produced immunoglobulin of the IgG2a subclass. Solid-phase absorption of anti-idiotype serum followed by competitive radioimmunoassay analyses revealed that all the idiotypic determinants recognized by anti-idiotype serum reside on this monoclonal antibody.
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001391:Azo Compounds / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D006822:Hybrid Cells / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D046911:Macromolecular Substances; D051379:Mice; D008805:Mice, Inbred A; D011817:Rabbits; D011863:Radioimmunoassay; D010132:p-Azobenzenearsonate / Q000276:immunology*
D000911:Antibodies, Monoclonal; D001391:Azo Compounds; D007106:Immune Sera; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D046911:Macromolecular Substances; D010132:p-Azobenzenearsonate