Quantitative cytochemistry of nuclear and cytoplasmic proteins using the Naphthol Yellow S and dinitrofluorobenzene staining methods.
The 'total protein staining' of biological specimens with the electrostatically binding Naphthol Yellow S or the covalently binding dinitrofluorobenzene must be interpreted as methods which yield data on the specific amino acid pool of the proteins concerned. Both dyes bind to certain free amino-acid side-chains, giving different dye--protein ratios for various proteins. In the presence of DNA, dinitrofluorobenzene stains all proteins present in cell nuclei, whereas Naphthol Yellow S only stains the majority of the non-histone proteins. When protein staining methods are combined with the Feulgen--Pararosanile (SO2) procedure for DNA, decreased Feulgen--DNA contents were measured in dinitrofluorobenzene-stained isolated nuclei and lymphocytes.
D000818:Animals; D001665:Binding Sites; D002467:Cell Nucleus / Q000032:analysis*; D004396:Coloring Agents; D004139:Dinitrofluorobenzene; D008099:Liver / Q000166:cytology; D009282:Naphthalenesulfonates; D009578:Nitrobenzenes; D009698:Nucleoproteins / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D051381:Rats
D004396:Coloring Agents; D009282:Naphthalenesulfonates; D009578:Nitrobenzenes; D009698:Nucleoproteins; D011506:Proteins; C009824:naphthol yellow; D004139:Dinitrofluorobenzene