The proportion of ribosomes engaged in rat pancreatic polysomal structures as influenced by pilocarpine injection.
Rats intravenously injected with pilocarpine at a concentration of 4 mg per 100 g body weight were killed after various time intervals and the relative numbers of ribosomes per cell active in protein synthesis were determined. It was found that until 1 h after pilocarpine injection there is a significant decrease in the number of ribosomes engaged in protein synthesis, whereas 2--3 h after pilocarpine injection, the number of active ribosomes equals that of control animals. After a short incubation in vitro, the same relative number of active ribosomes is always found, independent of the value determined in the pancreas directly after the death of the animal.
D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D010862:Pilocarpine / Q000494:pharmacology*; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012270:Ribosomes / Q000187:drug effects* / Q000378:metabolism
D010862:Pilocarpine