A superfusion method for the study of secretion activity of isolated cerebral and endocrine tissues.
This paper describes a new kind of superfusion technique that allows for the maintenance of small fragments of nervous tissue, such as of the hypothalamus and the pituitary and pineal glands, suspended in a very small perfusion chamber with a continuous flow of medium that reproduces "in vivo" conditions. Easy reproducibility; continuous flow of medium, which avoids short feedback phenomena; controlled conditions that are very close to those "in vivo" and the possibility of sequential treatments for the study of the dynamics of hormone release are some of the advantages of this method.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D004702:Endocrine Glands / Q000378:metabolism*; D005260:Female; D007031:Hypothalamus / Q000378:metabolism; D066298:In Vitro Techniques; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D010870:Pineal Gland / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D011388:Prolactin / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D011388:Prolactin