A rapid method for the determination of urea stable lactate dehydrogenase on the 'Cobas Bio' centrifugal analyser.
The urea-stable lactate dehydrogenase assay has been adapted to the 'Cobas Bio' centrifugal analyser. It was found that incubation of the serum samples with 2 mol/l urea for 2 min was sufficient for inhibition of the LD liver isoenzymes. Under these conditions enzyme activity of cardiac origin was mainly unaffected. The method is fully automated, rapid and inexpensive.
D002498:Centrifugation / Q000379:methods; D004355:Drug Stability; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000201:enzymology; D008107:Liver Diseases / Q000201:enzymology; D009203:Myocardial Infarction / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D011933:Reagent Kits, Diagnostic; D014508:Urea / Q000494:pharmacology*
D011933:Reagent Kits, Diagnostic; D014508:Urea; D007770:L-Lactate Dehydrogenase