Neuronotrophic factors and their antibodies: in vitro microassays for titration and screening.
Using nerve growth factor (NGF), anti-NGF sera and dissociated neonatal mouse dorsal root ganglionic neurons we present a microculture assay methodology for (1) the titration of neurotrophic factor (NTF) activity in monolayer culture, (2) the titration of NTF antibodies which 'block' NTF biological activity, (3) the titration of NTF antibodies that bind and remove (sequester) NTF from culture medium and (4) a large-scale, convenient, and rapid screening for NTF biological activity as well as for NTF 'blocking' or 'sequestering' antibodies. These quantitative and qualitative in vitro microimmunoassays should be applicable to any neuronotrophic factor or its antibody, even when the agent is only available in crude, unpurified form. Since the microculture systems permit the simultaneous screening of one thousand samples per day they should be useful for the detection and quantitation of monoclonal antibodies present in hybridoma-conditioned media.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D000936:Antigen-Antibody Complex / Q000032:analysis; D001667:Binding, Competitive; D046508:Culture Techniques; D005727:Ganglia, Spinal / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009414:Nerve Growth Factors / Q000276:immunology*; D009419:Nerve Tissue Proteins / Q000276:immunology*; D009474:Neurons / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins