[Effect of selectively reacting ligands on the helix-coil transition of DNA. IV. Heat denaturation of DNA in an acid medium].
The DNA thermal denaturation in acidic medium has been investigated experimentally and theoretically. The formulae describing a dependence of the helix--coil transition parameters (melting temperature (Tm) and melting range width (delta T) on ionisation constants values of all kinds of DNA bases in the helix and coil regions and medium acidity have been obtained. Dependences Tm (pH) and delta T (pH) have been determined experimentally and calculated for different models of protonation. Based on the comparison of theoretical and experimental dependences Tm (pH) and delta T (pH) a strict examination of the theory is conducted. The mechanism of DNA protonation is discussed.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000302:isolation & purification; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008433:Mathematics; D008956:Models, Chemical; D009691:Nucleic Acid Denaturation; D013950:Thymus Gland / Q000032:analysis
D008024:Ligands; D004247:DNA
Vliianie ligandov s izbiratel'nym kharakterom vzaimodeĭstviia na perekhod spiral'-klubok DNK. IV. Teplovaia denaturatsiia DNK v kisloĭ srede.