A polypeptide antigen from a strain of Staphylococcus simulans. 1. Purification and some chemical data.
A polypeptide antigen isolated from Staphylococcus simulans was shown mainly to contain alanine and aspartic acid in a molar ratio of 3:1. The circular dichroism spectre showed only positive ellipticities, and oxidation by D-amino acid oxidase showed the presence of D-alanine. 65 per cent of the alanine was present as the D-isomer as estimated by gas-liquid chromatography. Analytical ultracentrifugation showed that the polymer sedimented as a homogeneous peak with an S20, W value of 2.8.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000276:immunology; D013210:Staphylococcus / Q000276:immunology*
D000596:Amino Acids; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D010455:Peptides