Identification of Limulus polyphemus haemocyanin messenger RNA.
Total RNA was isolated from cyanoblast-containing tissue taken from behind the compound eye of the horseshoe crab, Limulus polyphemus. Poly(A)-containing RNA separated from this by affinity chromatography on oligo(dT)-cellulose was translated in the rabbit reticulocyte haemolysate system in the presence of L-[35S]methionine. By using an antiserum to Limulus haemocyanin, polypeptides were isolated from the translation products which had a similar mobility to the authentic Limulus haemocyanin polypeptides as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006433:Hemocyanins / Q000096:biosynthesis*; D006737:Horseshoe Crabs / Q000032:analysis*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*
D012333:RNA, Messenger; D006433:Hemocyanins