[Action of mutant genes on crystallin synthesis in the developing mouse lens. III. The aphakia gene].
The synthesis of crystallins was studied by means of indirect immunofluorescence in 10-18 days old embryos and newborn ak/ak mice. Immune gamma-globulins to alpha-, beta- and gamma-crystallins of adult mice were used. A small amount of alpha-crystallins was found in an abnormal lens vesicle of 14 days old ak/ak embryo. The initiation of synthesis of these proteins is, hence, delayed by 4 days, as compared with that in the normal mice. In 15 days old mutant embryos the amount of alpha-crystallins increased markedly and was kept at about the same level till the end of embryogenesis. In the newborn ak/ak mice, the lens material containing alpha-crystallins is distributed among the retinal folds and is not readily identified without immunofluorescence. No lens material was found in the adult mutant mice. gamma- and beta-crystallins were not found in the abnormal lens at all developmental stages studied.
D000818:Animals; D000831:Animals, Newborn; D001035:Aphakia / Q000196:embryology / Q000235:genetics* / Q000378:metabolism; D003459:Crystallins / Q000096:biosynthesis*; D005796:Genes; D007908:Lens, Crystalline / Q000196:embryology* / Q000254:growth & development / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D009154:Mutation
D003459:Crystallins
Izuchenie deĭstviia mutantnykh genov na sintez kristallinov v formiruiushchemsia khrustalike myshi. Soobshchenie III. Gen aphakia.