Biochemical changes during reticulocyte maturation in culture. A comparison of genetically different sheep erythrocytes.
A simple culture technique was used to follow sheep reticulocyte maturation. Cells remained in good condition without lysis for at least 7 days and maintained normal intracellular ATP concentrations. Amino acid and nucleoside transport were measured during maturation together with changes in reduced glutathione (GSH), amino acid, K+ and Na+ concentrations. Reticulocytes of the genetic types high-K+ and low-K+, high-GSH and low-GSH; nucleoside-permeable and nucleoside-impermeable were indistinguishable both in transport properties and cellular constituents. Cell maturation was associated with marked decreases in L-alanine transport (Na+-dependent and independent), uridine transport and GSH and K+ concentrations. Typical mature-cell levels for all parameters were reached within the culture period, during which time predicted differences between cells of different genotypes became apparent. Loss of amino acid-transport activity from low-GSH cells was accompanied by an increase in intracellular ornithine and lysine concentrations. The rates at which different parameters changed during culture were compared.
D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured; D004906:Erythrocyte Count; D005978:Glutathione / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D012156:Reticulocytes / Q000378:metabolism*; D012756:Sheep / Q000097:blood* / Q000235:genetics; D012964:Sodium / Q000378:metabolism
D000596:Amino Acids; D012964:Sodium; D005978:Glutathione; D000409:Alanine; D011188:Potassium