[Arrest of BHK-21 cell culture growth and its stabilization in a resting state as affected by serum fractions].
By cytofluorometry the distribution of cells was studied according to the amount of DNA in a population of BHK-21 cells placed in the media containing a serum fraction that either does not stimulate cell proliferation, or stimulates it until reaching 60% of the limiting density observed in the control. The analysis of cell distribution shown that after the arrest of cell growth cells are accumulated in the G1 phase of the cell cycle to change into the quiescent state. As a control, cytofluorometric analysis of populations of BHK-21 cells cultured under normal conditions in the presence of the 10% whole serum was performed.
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects*; D006224:Cricetinae; D004247:DNA / Q000032:analysis; D007668:Kidney; D008970:Molecular Weight
D001798:Blood Proteins; D004247:DNA
Ostanovka rosta kul'tur kletok BHK-21 i ikh stabilizatsiia v sostoianii pokoia pod deĭstviem syvorotochnykh fraktsiĭ.