Quantification of nuclear non-histone proteins by Feulgen--Naphthol Yellow S cytophotometry.
Owing to the accumulation of nuclear non-histone protein (NHP) (a) in cells entering the cell cycle from the quiescent state and (b) in continuously cycling cells during G1 phase, a simultaneous determination of DNA and nuclear NHP is of high potential utility in cell kinetic studies. This paper provides guidelines for a Feulgen--Naphthol Yellow S staining technique for this purpose. It discusses details of the preparation and quantification procedures, and reviews the evidence for a quantitative relationship between nuclear Naphthol Yellow S binding and nuclear NHP.
D000818:Animals; D002453:Cell Cycle; D002467:Cell Nucleus / Q000032:analysis; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D004396:Coloring Agents; D008099:Liver / Q000166:cytology; D009282:Naphthalenesulfonates; D051381:Rats; D012394:Rosaniline Dyes
D002868:Chromosomal Proteins, Non-Histone; D004396:Coloring Agents; C007722:Feulgen stain; D009282:Naphthalenesulfonates; D012394:Rosaniline Dyes; C009824:naphthol yellow