title: The use of combined high performance liquid chromatography negative ion chemical ionization mass spectrometry to confirm the administration of synthetic corticosteroids to horses.
abstract: Negative ion chemical ionization mass spectra of some corticosteroids have been obtained by direct syringe introduction on to the Finnigan moving belt high-performance liquid chromatography-mass spectrometer interface. Proprietary preparations based upon dexamethasone, betamethasone and prednisolone were administered to horses at therapeutic dose level. Urine samples were extracted, the extracts purified by Sephadex LH-20 chromatography and the presence of the parent steroids in the eluates was confirmed by combined high-performance liquid chromatography negative ion chemical ionization mass spectrometry using ammonia as reagent gas.
mesh_terms: D000305:Adrenal Cortex Hormones / Q000008:administration & dosage / Q000652:urine*; D000818:Animals; D001623:Betamethasone / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D003907:Dexamethasone / Q000652:urine; D004300:Doping in Sports; D006736:Horses / Q000652:urine*; D006854:Hydrocortisone / Q000652:urine; D007273:Injections, Intramuscular; D013058:Mass Spectrometry / Q000379:methods; D011239:Prednisolone / Q000652:urine
chemical_list: D000305:Adrenal Cortex Hormones; D003907:Dexamethasone; D001623:Betamethasone; D011239:Prednisolone; D006854:Hydrocortisone