[Determination of the optimal conditions for explantation of portions of the liver].
The authors explanted liver organ cultures from mice, 1 to 3 months old, of the BALB/cY strain in three types of nutritive medium-pure nutritive medium 199; combined nutritive medium "A" (80% nutritive medium 199 and 20% of bovine serum as 400 ml of sol. glucose 40%, 7 mg of vit "C" and 20 mg of glutamine were added to 100 ml of the medium) and combined nutritive medium "B" (400 mg of sol. glucose 40% was added to 100 ml of the nutritive medium 199). The usage of the combined nutritive medium "B" was the most suitable for continuous culture (42-48 days) of liver explantants with preservation of the morphological intactness of the tissue. They examined also the blastomogenic action of 0.200 mg/ml od diethylnitrozoamine on liver organ cultures. It was established that 0.200 ml/ml of diethylnitrozoamine was nontoxic dose on liver organ cultures, but without manifested blastomogenic effect.
D000818:Animals; D003470:Culture Media; D004052:Diethylnitrosamine / Q000494:pharmacology; D008099:Liver / Q000033:anatomy & histology* / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009924:Organ Culture Techniques / Q000379:methods*; D013997:Time Factors; D014022:Tissue Survival / Q000187:drug effects
D003470:Culture Media; D004052:Diethylnitrosamine
Opredeliane na optimalni usloviia za eksplantatsiia na kŭscheta ot cheren drob.