The metabolism of canrenone in vitro by rat liver preparations.
1. The metabolism of [1-3H]canrenone, a primary metabolite of spironolactone and potassium canrenoate, by rat liver preparations in vitro has been investigated. 2. Canrenone was metabolized by 3-oxo-delta 4-reduction to give 3 alpha-hydroxy-5 beta-spirolactones, and also by a number of O2 and NADPH-dependent microsomal hydroxylation reactions. 3. A major metabolic route requiring the presence of a microsomal fraction, but apparently independent of oxygen and NADPH, led to the formation of a number of compounds tentatively identified as trihydroxy-spirolactones.
D000818:Animals; D001711:Biotransformation; D002192:Canrenone / Q000378:metabolism*; D002248:Carbon Monoxide; D005609:Free Radicals; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D011245:Pregnadienes / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism
D005609:Free Radicals; D011245:Pregnadienes; D002192:Canrenone; D002248:Carbon Monoxide