[DNA synthesis in the process of rat spermatogenesis].
The rat spermatogonia were fractionated in STA-PUT system within 24 hrs following 3H-thymidine injection. DNA isolated from the cells at certain stages of spermatogenesis (spermatogonia, leptotene-zygotene, pachytene, spermatids-spermatozoa) was studied by the method of reassociation kinetics and ultracentrofugation in CsCl gradient. A selective 3H-thymidine incorporation in the region of midrepetitious nucleotide sequences was found at all stages under study, except spermatids-spermatozoa. DNA synthesized during spermatogenesis forms in CsCl density gradient several "satellite" peaks in the "heavy" and "light" gradient zones.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis* / Q000302:isolation & purification; D007700:Kinetics; D008297:Male; D009693:Nucleic Acid Hybridization; D051381:Rats; D011919:Rats, Inbred Strains; D013087:Spermatids / Q000378:metabolism; D013091:Spermatogenesis; D013093:Spermatogonia / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D013936:Thymidine
Sintez DNK v protsesse spermatogeneza u krys.