D-glucose uptake in human liver cell cultures.
The kinetics parameters for D-glucose uptake were studied in human liver cell cultures under strictly defined experimental conditions. Using a wide concentration range (0.005 to 30 mmol/l), the kinetic data obtained suggested strongly that D-glucose in human liver cell cultures can be transported by two separate systems. For the high-affinity system, the apparent Km was 0.645 +/- 0.21 mmol/l and the Vmax, 12.49 +/- 3.74 nmol/mg protein per min. For the low-affinity system, the apparent Km was 6.91 +/- 0.58 mmol/l and the Vmax, 79.90 +/- 5.27 nmol/mg protein per min. At a concentration of 2.1 x 10(-7) mol/l, cytochalasin B preferentially inhibited the high-affinity D-glucose site or transport system. The time course of D-glucose uptake, studied in two cell lines from patients with hereditary fructose intolerance, was significantly higher than for the control lines.
D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D003571:Cytochalasin B / Q000494:pharmacology; D005633:Fructose Intolerance / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000378:metabolism*
D003571:Cytochalasin B; D005947:Glucose