Chemical and biological properties of subfractions of hot-water extract from delipidated BCG.
a hog-water extract from delipidated Mycobacterium bovis strains BCG was fractionated by precipitation with ethanol. The chemical and biological properties of the subfractions thus obtained were investigated. A subfraction precipitated at 20% ethanol contained less neutral sugar and more ninhydrin-positive substance than other subfractions. Only the guinea pigs that were treated with 20% ethanol-precipitate showed no humoral antibody response to egg white albumin, despite positive skin reaction. The subfractions precipitated at low concentrations of ethanol exhibited a significant antitumor activity in ddY and C3H He mice inoculated intraperitoneally with tumor cells. These results suggest that the hot-water extract from delipidated BCG consists of subfractions with different chemical and biological properties.
D000276:Adjuvants, Immunologic / Q000032:analysis; D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis; D001500:BCG Vaccine / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D006168:Guinea Pigs; D008055:Lipids; D008297:Male; D051379:Mice; D009374:Neoplasms, Experimental / Q000188:drug therapy
D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D001500:BCG Vaccine; D008055:Lipids