title: Attempted direct visualization of negatively stained amplified immune complex of synaptic acetylcholinesterase using cryoultramicrotomy sections.
abstract: An immunocytochemical method is proposed for the localization of synaptic acetylcholinesterase (AChE) on ultrathin frozen sections of the electric organ of the electric eel. The immune complex formed is amplified by a non-specific "sandwich" technique and visualized by negative staining. Definite white spots on synaptic cleft seem to correspond to basal lamina AChE molecules.
mesh_terms: D000110:Acetylcholinesterase / Q000032:analysis*; D000818:Animals; D000936:Antigen-Antibody Complex; D004557:Electric Organ / Q000201:enzymology*; D004593:Electrophorus; D006651:Histocytochemistry; D007120:Immunochemistry / Q000379:methods*; D008854:Microscopy, Electron; D011817:Rabbits
chemical_list: D000936:Antigen-Antibody Complex; D000110:Acetylcholinesterase