title: Influence of pH and ionic strength on the lysis of Micrococcus luteus cells by hen lysozyme at low (20 degree C) and high (physiological, 40 degree C) temperature.
abstract: From isoactivity curves (showing activity as a function of Ph and ionic strength) it was found that in the pH domain 6.7-8.6 frequently used in experiments involving hen lysozyme, the pH optimum of lysis of Micrococcus luteus cells at low ionic strength (0.02-0.05) by the high-temperature form (40 degree C, physiological temperature) was one to two pH units lower than that by the low-temperature form (20 degree C).
mesh_terms: D000818:Animals; D002645:Chickens; D006863:Hydrogen-Ion Concentration; D008837:Micrococcus / Q000378:metabolism*; D009113:Muramidase / Q000378:metabolism*; D009994:Osmolar Concentration; D013696:Temperature
chemical_list: D009113:Muramidase