[Isolation of "Toxoplasma gondii" membrane antigens using latex microspheres (author's transl)].
The authors describe a method for isolation of Toxoplasma gondii antigenic membrane components. Technical work is described into four points: 1) specific labelling of membrane proteins was realised by DD125ISA; 2) methacrylate microspheres were bound to unbroken toxoplasma membranes by the mean of an indirect technic with antibody molecules (double sandwich); these microspheres were used to modify the membrane density; 3) toxoplasmas bound to microspheres were broken by sonication; 4) microspheres fixed to membrane components were isolated by isopycnic ultracentrifugation on continuous sucrose gradient.
D000954:Antigens, Surface / Q000302:isolation & purification*; D008689:Methacrylates; D008722:Methods; D008863:Microspheres; D014122:Toxoplasma / Q000276:immunology*
D000954:Antigens, Surface; D008689:Methacrylates
Isolement de fractions antigéniques membranaires de Toxoplasma gondii par l'intermédiaire de microsphères de méthacrylate.