Dansyl (5-dimethylaminonaphthalene-1-sulphonyl)-heparin binds antithrombin III and platelet factor 4 at separate sites.
Antithrombin III binds to, and thereby augments the fluorescence of, dansyl-(5-dimethylaminonaphthalene-1-sulphonyl)-heparin; platelet factor 4 binding to the fluorescent heparin has little of this effect. Competition studies in which antithrombin III competes with platelet factor 4 for heparin binding demonstrate that heparin can simultaneously bind both proteins.
D000990:Antithrombin III / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D001779:Blood Coagulation Factors / Q000378:metabolism*; D006493:Heparin / Q000031:analogs & derivatives* / Q000378:metabolism; D010978:Platelet Factor 4 / Q000378:metabolism*; D011485:Protein Binding; D013050:Spectrometry, Fluorescence
D001779:Blood Coagulation Factors; C032585:dansyl-heparin; D010978:Platelet Factor 4; D000990:Antithrombin III; D006493:Heparin