An endogenous dinucleotide bound to diphtheria toxin. Adenylyl-(3',5')-uridine 3'-monophosphate.
Diphtheria toxin has recently been fractionated into two forms, one of which contains tightly but noncovalently bound nucleotide-like material. Here we report identification of the major nucleotide constituent (comprising 80% of the total extractable ultraviolet-absorbing material) as adenylyl-(3',5')-uridine 3'-monophosphate (ApUp). Upon incubation with ApUp, the nucleotide-free form of toxin bound approximately one molar equivalent of the dinucleotide and was converted to a form similar or identical with the native nucleotide-bound form.
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D015226:Dinucleoside Phosphates; D004167:Diphtheria Toxin; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet
D015226:Dinucleoside Phosphates; D004167:Diphtheria Toxin; D007202:Indicators and Reagents; C006413:adenylyl-(3'-5')-uridine; D000249:Adenosine Monophosphate