Double-label reductive methylation of tissue proteins for precision two-dimensional polyacrylamide-gel electrophoretic analysis.
Reductive methylation has been used to radioactively label crude-extract proteins with 3H or 14C. The procedure achieved good isotope incorporation and resolution of proteins on two-dimensional polyacrylamide gels. It allows high-precision comparison of tissue samples by double-labelling and should facilitate the study of tissue proteins by two-dimensional gel electrophoresis.
D000818:Animals; D002250:Carbon Radioisotopes; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D008099:Liver / Q000032:analysis; D008745:Methylation; D051379:Mice; D010084:Oxidation-Reduction; D011506:Proteins / Q000032:analysis*; D014316:Tritium
D002250:Carbon Radioisotopes; D011506:Proteins; D014316:Tritium