A rapid and inexpensive radioimmunoassay for parathyroid hormone utilizing the Wide-principle in combination with an improved separation technique.
A radioimmunoassay of PTH which utilizes the "Wide-principle", i.e. a low concentration of antibody and a very high concentration of label, is presented. The detection limit was 0.06 micrograms/l. S-PTH in a reference group (n = 30) was 0.06-0.6 micrograms/l and the coefficient of variation was 7 and 11% at 0.8 micrograms/l (intra-day and inter-day variation). The duration of the assay is two days. The separation system involves precipitation with 20% PEG, re-suspension of the precipitate in saline followed by re-precipitation with PEG. The non-specifically bound radioactivity is reduced to 2-5%. The assay is relatively insensitive to variations in non-specifically bound radioactivity and "incubation damage". It is suitable for use in a routine laboratory for the evaluation of parathyroid gland function under various conditions. An important property of the assay is that antibody consumption is reduced, with consequent reduction in overall costs.
D000328:Adult; D002118:Calcium / Q000378:metabolism; D005260:Female; D006801:Humans; D007011:Hypoparathyroidism / Q000097:blood; D008297:Male; D010280:Parathyroid Glands / Q000502:physiology; D010281:Parathyroid Hormone / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods*
D010281:Parathyroid Hormone; D002118:Calcium