Epoxide hydrolase activity in human skin.
1 Epoxide hydrolase (EH) activity was measured in biopsied skin (n = 42) using 7-[H3]-styrene oxide as substrate, and separation of the products by high performance liquid chromatography. 2 EH activity (mean +/- s.d.) was present in separated epidermis (139 +/- 105 pmol glycol formed mg-1 min-1) and dermis (165 +/- 120 pmol glycol formed mg-1 microsomal protein min-1). 3 Whole skin EH activity (mean +/- s.d.) varied widely (433 +/- 254 pmol glycol formed mg-1 microsomal protein min-1) 4 No significant difference in EH activity was observed in skin from breast, penis and leg. 5 Skin EH activity does not appear to contribute significantly to the systemic metabolism of epoxide, but may be important in determining the effects of epoxides formed within the epidermis.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D004817:Epidermis / Q000201:enzymology; D004851:Epoxide Hydrolases / Q000032:analysis*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008875:Middle Aged; D012867:Skin / Q000201:enzymology*
D004851:Epoxide Hydrolases