A morphometrical method for analyzing germinal centers in the chicken spleen.
A morphometric method for the analysis of the germinal centers in the chicken spleen is presented. Based on counts in the two-dimensional tissue sections, the following three-dimensional germinal center characteristics were calculated: volume density (VVgc); numerical density (NVgc); total number in the spleen (Ngcsp); nearest neighbour distance (delta 3), and mean diameter (D). The total number of the germinal centers in the spleen of the six-week-old and the ten-week-old unimmunized chickens was 803 and 642, respectively. The mean diameter of the germinal centers was 102-108 micrometer, but its size distribution ranged from 50 to 175 micrometer.
D000818:Animals; D002645:Chickens / Q000033:anatomy & histology*; D006410:Hematopoiesis; D008214:Lymphocytes / Q000166:cytology*; D008433:Mathematics; D008853:Microscopy; D013154:Spleen / Q000166:cytology*