Inhibition of the pokeweed mitogen-induced response of normal peripheral blood lymphocytes by humoral components of colostrum.
Colostral whey at dilutions up to 1 : 100 inhibited both the uptake of tritium-labelled thymidine and the differentiation of pokeweed mitogen-stimulated peripheral blood lymphocytes from normal adults into immunoglobulin-containing plasma cells. Upon gel filtration (Sephadex G-200), inhibitory activity was associated with high molecular weight fractions. Secretory component, but not monomeric, polymeric or colostral IgA, IgM, IgG, lactoferrin, casein or alpha-lactalbumin, inhibited the response to mitogen. Supernatants from cultures of colostral cells did not induce the differentiation of adult peripheral or cord blood lymphocytes into IgA-containing cells and did not stimulate the uptake of tritium-labelled thymidine.
D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002850:Chromatography, Gel; D003126:Colostrum / Q000276:immunology*; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D008894:Milk Proteins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D012635:Secretory Component
D007136:Immunoglobulins; D008894:Milk Proteins; D011043:Pokeweed Mitogens; D012635:Secretory Component; D004247:DNA