Amniotic fluid fluorescence polarisation values for assessing fetal lung maturation.
The fluorescence probe 1,6, diphenyl-1,3,5-hexatriene was used to determine the micro-viscosity of third trimester  amniotic fluid samples utilising an Aminco Bowman spectrofluorometer fitted with a polariser prism. The results were related to the lecithin sphingomyelin (L:S) ratio. A fluorescence polarisation value of 0.360 corresponded to an L:S ratio of 2.0 in uncomplicated and diabetic pregnancies. Pregnancies complicated by Rhesus disease gave significantly different fluorescence polarisation values when compared with the uncomplicated pregnancies.
D000653:Amniotic Fluid / Q000032:analysis / Q000502:physiology*; D004161:Diphenylhexatriene; D005260:Female; D005325:Fetal Organ Maturity; D005454:Fluorescence Polarization / Q000379:methods; D006801:Humans; D008168:Lung / Q000196:embryology*; D010713:Phosphatidylcholines / Q000032:analysis; D011247:Pregnancy; D013109:Sphingomyelins / Q000032:analysis; D014783:Viscosity
D010713:Phosphatidylcholines; D013109:Sphingomyelins; D004161:Diphenylhexatriene