Activation of latent ribonuclease in the fat-body of fleshfly (Sarcophaga peregrina) larvae on pupation.
A crude extract of the fat-bodies of third-instar larvae of Sarcophaga peregrina (fleshfly) was found to contain latent RNAase (ribonuclease) consisting of RNAase and inhibitor protein that is sensitive to p-chloromercuribenzoic acid. The RNAase activity in the crude extract of fat-bodies became detectable with time after puparium formation, indicating that the inhibitor is selectively inactivated and RNAase is released from the RNAase-inhibitor complex during metamorphosis.
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D002499:Centrifugation, Density Gradient; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004175:Diptera / Q000201:enzymology* / Q000254:growth & development; D004789:Enzyme Activation / Q000187:drug effects; D005216:Fat Body / Q000187:drug effects / Q000201:enzymology*; D066298:In Vitro Techniques; D007814:Larva / Q000201:enzymology; D011679:Pupa / Q000201:enzymology; D012260:Ribonucleases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D020245:p-Chloromercuribenzoic Acid
D002729:Chloromercuribenzoates; D020245:p-Chloromercuribenzoic Acid; D012260:Ribonucleases