[Relation between changes in platelet shape, calcium balance and the separating effect of cytochalasin B].
Platelets were stimulated in vitro by different ADP concentration (10(-7)--10(-9) M). Scanning electron microscopy, light transmission recording and the Ca-sensitive fluorescent probe, chlortetracycline, were used to study the time course of changes in the shape, aggregation and the amount of membrane-bound calcium during ADP activation. It was found that ADP (10(-7)--10(-8)M) causes reversible changes in the shape of platelets without disturbing their aggregation. The changes in question were accompanied by phasic alterations of the amount of membrane-bound calcium.
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000818:Animals; D001792:Blood Platelets / Q000166:cytology*; D002118:Calcium / Q000378:metabolism*; D003285:Contractile Proteins / Q000378:metabolism; D003571:Cytochalasin B / Q000494:pharmacology*; D008566:Membranes / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010974:Platelet Aggregation; D011485:Protein Binding; D051381:Rats
D003285:Contractile Proteins; D003571:Cytochalasin B; D000244:Adenosine Diphosphate; D002118:Calcium
Sviaz' izmeneniĭ formy trombotsitov s balansom kal'tsiia i razobshchaiushchiĭ éffekt tsitokhalazina B.