title: The determination of O,S,S-trimethylphosphorodithioate in the plasma and various tissues of rats using high-resolution gas chromatography with nitrogen-phosphorus detection.
abstract: A method has been developed for the determination of O,S,S-trimethylphosphorodithioate in the plasma, lung, liver, brain and thymus of rats using high-resolution gas chromatography. The organophosphorus compound was extracted from the biological sample with ethyl acetate and analysed on a carbowax 20M fused-silica capillary column with a nitrogen-phosphorus specific detector. O,S,S-Triethylphosphorodithioate was used as an internal standard added to the sample before extraction. The sensitivity of the method allowed the compound to be measured in 0.1-ml aliquots of plasma or in 20-mg wet weight of tissue down to a level of 5 ng/sample. The method has been applied to a pharmacokinetic study in the rat after an oral or intravenous dosage with 25 mg/kg of O,S,S-trimethylphosphorodithioate.
mesh_terms: D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D005260:Female; D009584:Nitrogen; D063086:Organothiophosphates / Q000032:analysis* / Q000097:blood; D009946:Organothiophosphorus Compounds / Q000032:analysis*; D010758:Phosphorus; D051381:Rats; D011919:Rats, Inbred Strains; D014018:Tissue Distribution
chemical_list: D063086:Organothiophosphates; D009946:Organothiophosphorus Compounds; C033125:O,S,S-trimethyl phosphorodithioate; D010758:Phosphorus; D009584:Nitrogen