Automated enzymatic determination of ATP in bovine muscle tissue.
An automated method is described for the enzymatic determination of adenosine-5'-triphosphate (ATP) in meat using hexokinase (EC 2.7.1.1) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) in the range of 0-115 micrograms/ml. THe NADPH formed is measured spectrophotometrically at 340 nm. ATP can be analysed at a rate of forty samples per hour.
D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D001322:Autoanalysis; D002417:Cattle; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D008460:Meat / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000255:Adenosine Triphosphate; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase