Determination of acrylamide in nerve tissue homogenates by electron-capture gas chromatography.
Acrylamide in biological samples can be determined by gas chromatography with electron-capture detection after conversion to its 2,3-dibromopropionamide derivative. The derivatization is carried out in aqueous solution, plasma or tissue homogenates by ionic bromination and the reaction conditions and sample clean-up are described. The detection limit corresponds to 9.5.10(-12)g of acrylamide on column or 8.4.10(-9)g in the final biological extract (0.5 ml). The overall recovery of acrylamide spiked samples at the nanogram level exceeds 80%. It was found that the accumulation of free acrylamide in the sciatic nerve distal region of rats intoxicated with acrylamide was less than 2-8 ppm.
D000178:Acrylamides / Q000032:analysis* / Q000097:blood / Q000633:toxicity; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D007279:Injections, Subcutaneous; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012584:Sciatic Nerve / Q000032:analysis* / Q000187:drug effects / Q000473:pathology; D013696:Temperature
D000178:Acrylamides