Single cells from adult mammalian heart: isolation procedure and preliminary electrophysiological studies.
Single ventricular cells have been isolated from adult guinea pig heart using a collagenase enzyme method. The procedure is straightforward, takes about 20 min, and yields 40-65% rod-shaped cells which are quiescent in millimolar Ca2+ solution. Electrophysiological studies involving external ion changes and ionic channel inhibitors suggest that the cells retain their active membrane properties following this enzymatic dissociation.
D000200:Action Potentials; D000818:Animals; D002469:Cell Separation / Q000379:methods; D004594:Electrophysiology; D006168:Guinea Pigs; D006321:Heart / Q000502:physiology*; D066298:In Vitro Techniques; D008564:Membrane Potentials; D009206:Myocardium / Q000166:cytology*