[HLA-typing of bloodstains. Investigations with an absorption tests. Preliminary report (author's transl)].
Report on an absorption test, in which the antigens HLA-A1,-A3, -A9, -B7, and -B8 were investigated with four different antisera each. 0.5 mg bloodstain (on cotton thread; age: between 7 and 21 days) was added to 5 microliters pretitrated antiserum. After incubation (14h at 4 degrees C) it was tested whether absorption had occurred or not: 1 microliter antiserum was placed in microtest plate and 1 microliter target lymphocyte suspension was added. The further test followed the NIH-technique. The presence of an antigen is shown by a reduction of the complement-mediated cytotoxicity. The results show that cross reactions play a more important role than in the NIH-test. In our investigations over 90% of the HLA-antigens were typed correctly.
D001788:Blood Grouping and Crossmatching / Q000379:methods*; D001801:Blood Stains; D006680:HLA Antigens / Q000032:analysis*; D006801:Humans
D006680:HLA Antigens
HLA-Typisierung von Blutspuren. Untersuchungen mit einem Absorptionstest.