title: A simple procedure for partial purification of an RNAase of Entamoeba invadens.
abstract: The behavior of an RNAase, present in centrifugally clarified homogenates of axenic trophozoites of Entamoeba invadens, on isoelectric focusing (IEF) and agarose-poly(G) chromatography is described. The results led to a simple two-step procedure for partial purification of the RNAase in which fractionation of the homogenate by IEF is followed by agarose-poly(G) chromatography. Recovery of enzyme activity has ranged from 50 to 80% of that present in homogenates, and increases of 80- to 160-fold in the specific activity have been obtained using the procedure. A single zone of activity was observed on analysis of the partially purified RNAase by sucrose gradient IEF and velocity zonal ultracentrifugation.
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D002847:Chromatography, Agarose; D004747:Entamoeba / Q000201:enzymology*; D007525:Isoelectric Focusing; D008722:Methods; D012260:Ribonucleases / Q000302:isolation & purification*
chemical_list: D012260:Ribonucleases