Interaction of aromatic dyes with the coenzyme A binding site of choline acetyltransferase.
The interaction of a series of aromatic dyes with the coenzyme A binding site of choline acetyltransferase was studied. Several of the dyes were very potent inhibitors of the enzyme. With few exceptions, inhibition was competitive with respect to acetylcoenzyme A and noncompetitive with respect to choline. It appears likely that inhibition by dyes such as Reactive Blue 2 (Cibacron Blue F3GA) or Congo Red, as in the case of coenzyme A interactions, involves hydrophobic bonding, as well as a coulombic interaction with an arginine residue.
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002795:Choline O-Acetyltransferase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D003065:Coenzyme A / Q000378:metabolism*; D004396:Coloring Agents / Q000494:pharmacology*; D049832:Decapodiformes; D005724:Ganglia / Q000201:enzymology; D066298:In Vitro Techniques; D007700:Kinetics
D004396:Coloring Agents; D002795:Choline O-Acetyltransferase; D003065:Coenzyme A