[Comparison of inactivated rabies vaccines obtained from diploid and polyploid heterologous cells (Hak, BHK, and Vero)].
Homologous or heterologous diploid or polyploid cells have been established as good producers of virus. In this study we present three types of experimental inactivated antirabies vaccines obtained under identical conditions from three types of cells. The study concerns the following characteristics of the vaccines: protecting powers, rabies antigens and major polypeptidic components; this study was performed on supernatants of tissue cultures and concentrated and purified vaccines. Moreover, some cellular and serum contaminants as well as minor rabies proteins were analysed in the viral solutions. It, thus, appears that all these types of vaccines have a similar protecting power (Number of 50% protective doses/micrograms viral protein).
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004171:Diploidy; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera; D007668:Kidney; D011123:Polyploidy; D011819:Rabies Vaccines / Q000592:standards*; D011820:Rabies virus / Q000302:isolation & purification; D014764:Viral Proteins / Q000032:analysis
D007106:Immune Sera; D011819:Rabies Vaccines; D014764:Viral Proteins
Etude comparative de vaccins rabiques inactivés obtenus à l'aide de cellules hétérologues diploides et polyploides (Hak, BHK et Véro).