Radiometric--microbiologic assay of vitamin B-6: application to food analysis.
A radiometric microbiologic assay for vitamin B-6 was applied to food analysis. The method was shown to be specific, reproducible and simpler than the standard turbidimetric microbiologic technique. The analysis of seven commercially available breakfast cereals was compared to a high performance liquid chromatography method. Three out of the seven cereals agreed when assayed with both methods (P greater than 0.1). Four cereals, however, differed in value considerably (P less than 0.05). Further studies are required to determine whether these differences were due to different extraction procedures used. The study showed that the new radiometric-microbiologic method can be used to measure total vitamin B-6 or, combined with a column separation procedure, to analyze for specific forms of the vitamin.
D001681:Biological Assay; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D002523:Edible Grain / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007225:Infant Food / Q000032:analysis*; D011736:Pyridoxine / Q000032:analysis*; D014633:Valine / Q000378:metabolism; D015003:Yeasts / Q000378:metabolism*
D002250:Carbon Radioisotopes; D014633:Valine; D011736:Pyridoxine