[Use of polyethylene glycol for the microinjection of exogenous protein into cultured murine cells].
Polyethylene glycol was applied to microinject two exogenous proteins: bovine serum albumin and non-histone protein derived from mouse spleen chromatin, into the mouse L-cells. The effectiveness of fusion of mammal (human, in the given case) erythrocytes in which hemoglobin is substituted for the protein under study was shown to be higher than when Sendai virus was used. The microinjected proteins preserve their specificity to subcellular structures.
D000818:Animals; D002417:Cattle; D002459:Cell Fusion / Q000187:drug effects; D002478:Cells, Cultured; D002868:Chromosomal Proteins, Non-Histone / Q000008:administration & dosage*; D004912:Erythrocytes / Q000187:drug effects; D006801:Humans; D007739:L Cells / Q000187:drug effects*; D051379:Mice; D008845:Microinjections / Q000379:methods*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage*
D002868:Chromosomal Proteins, Non-Histone; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols
Ispol'zovanie poliétilenglikolia dlia mikroin"ektsii ékzogennogo belka v kul'tiviruemye kletki myshi.