Selective migration of 'null' cells towards a thymus factor in vitro.
Mouse fetal liver, nude adult bone-marrow and normal adult bone-marrow cells are depleted of theta+, Ig+ cells by the use of in vitro migration through a porous membrane towards a thymus supernatant attractant. The attracted cells are free in suspension, viable and can be obtained in large numbers through the use of multiple migration wells for in vivo testing.
D000818:Animals; D000941:Antigens; D001853:Bone Marrow / Q000503:physiopathology; D002448:Cell Adhesion; D002465:Cell Movement; D008215:Lymphocytes, Null / Q000502:physiology*; D051379:Mice; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell; D013154:Spleen / Q000276:immunology / Q000502:physiology; D013950:Thymus Gland / Q000276:immunology / Q000502:physiology*
D000941:Antigens; D011947:Receptors, Antigen, B-Cell