[Demonstration by chromatofocalization of isoenzymes of sheep brain fucosyltransferase].
Glycoprotein : fucosyltransferase from sheep brain has previously been solubilized with detergent Triton X 100 and prepurified by hydrophobic chromatography on ethyl-agarose. Additional purification was performed using a new method, namely chromatofocalization. With a small column (30 ml) of P B E 94 Pharmacia eluted by a mixture of polybuffers 76 and 94, we were able to separate quickly four isoenzymes of the cerebral fucosyltransferase in the pH range 5-8. With regard to speed and ease, this technique seems to be very useful in purification of glycosyltransferases.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D005647:Fucosyltransferases / Q000032:analysis*; D006602:Hexosyltransferases / Q000032:analysis*; D007527:Isoenzymes / Q000032:analysis*; D012756:Sheep
D007527:Isoenzymes; D005647:Fucosyltransferases; D006602:Hexosyltransferases
Mise en évidence par chromatofocalisation des isoenzymes de la fucosyl-transférase cérébrale d'agneau.