The construction, identification and partial characterization of plasmids containing guinea-pig milk protein complementary DNA sequences.
A complementary DNA (cDNA) plasmid library has been constructed in the plasmid pAT153, using poly(A)-containing RNA isolated from the lactating guinea-pig mammary gland as the starting material. Double stranded cDNA was inserted into the EcoRI site of the plasmid using poly(dA . dT) tails, then transformed into Escherichia coli HB101. From the resulting colonies we have selected and partially characterized plasmids containing cDNA copies of the mRNAs for casein A, casein B, casein C and alpha-lactalbumin. However, the proportion containing casein C cDNA was exceptionally low, and these contained at best 60% of the mRNA sequence.
D000818:Animals; D004247:DNA / Q000235:genetics*; D004274:DNA, Recombinant; D004587:Electrophoresis, Agar Gel; D005260:Female; D005786:Gene Expression Regulation; D006168:Guinea Pigs; D007774:Lactation; D008321:Mammary Glands, Animal / Q000032:analysis; D008894:Milk Proteins / Q000235:genetics*; D010957:Plasmids; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D008894:Milk Proteins; D012333:RNA, Messenger; D004247:DNA