Induction of target antigens and conversion to susceptible phenotype of NK-cell-resistant lymphoid cell line.
Two autologous Herpesvirus papio producer lymphoid cell lines and one autologous non-producer line were compared for susceptibility to natural killer (NK) cell-mediated lysis. The non-producer cell line, 26CB-1, was more resistant to NK cell killing compared to one viral producer counterpart 13CB-1, but equally resistant when compared to another, 8CB-1. Treatment with chemical agents that affect differentiation or activate the viral cycle, including n-butyrate, IuDR, 5-azacytidine and tunicamycin, increased the susceptibility to killing of the non-producer line but had less effect on the 13CB-1 producer line. The increase in susceptibility was due to induction of new target antigens: activated 26CB-1 cells were more effective at inhibiting NK-cell-mediated lysis and were bound by more NK cells than untreated control cells. The expression of NK target structures may be related to the differentiated state rather than to the viral production status to target cells.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D000954:Antigens, Surface / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D005838:Genotype; D006564:Herpesviridae / Q000276:immunology; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D010215:Papio; D010641:Phenotype
D000954:Antigens, Surface; D000956:Antigens, Viral