Determination of acetaldehyde in human blood by a gas chromatographic method with negligible artefactual acetaldehyde formation.
A method is described for determination of acetaldehyde in blood by head space gas chromatography. The method utilizes sodium nitrite-sulfosalicylic acid as an inhibitor of the ethanol oxidizing systems by means of which the interference of ethanol is reduced considerably. The detection limit was 0.4 mumol/l, the recovery 101.5 +/- 5.2% and the coefficient of variation was 7.8% (1.5 mumol/1 acetaldehyde). There was no disappearance of acetaldehyde if the head space vials were kept at -20 degree C for 24 h. In the comparison study with the semicarbazide method our results were 0.7-4.1 mumol/l lower. The values for acetaldehyde in blood after ethanol ingestion (0.5 g/kg) by volunteers were 0.5-1.3 mumol/l.
D000079:Acetaldehyde / Q000097:blood*; D001557:Benzenesulfonates; D001784:Blood Gas Analysis / Q000379:methods; D002849:Chromatography, Gas / Q000379:methods; D000431:Ethanol / Q000008:administration & dosage; D006801:Humans; D012459:Salicylates; D012663:Semicarbazides; D012977:Sodium Nitrite
D001557:Benzenesulfonates; D012459:Salicylates; D012663:Semicarbazides; D000431:Ethanol; D000079:Acetaldehyde; C003366:sulfosalicylic acid; D012977:Sodium Nitrite