Attempted direct visualization of negatively stained amplified immune complex of synaptic acetylcholinesterase using cryoultramicrotomy sections.
An immunocytochemical method is proposed for the localization of synaptic acetylcholinesterase (AChE) on ultrathin frozen sections of the electric organ of the electric eel. The immune complex formed is amplified by a non-specific "sandwich" technique and visualized by negative staining. Definite white spots on synaptic cleft seem to correspond to basal lamina AChE molecules.
D000110:Acetylcholinesterase / Q000032:analysis*; D000818:Animals; D000936:Antigen-Antibody Complex; D004557:Electric Organ / Q000201:enzymology*; D004593:Electrophorus; D006651:Histocytochemistry; D007120:Immunochemistry / Q000379:methods*; D008854:Microscopy, Electron; D011817:Rabbits
D000936:Antigen-Antibody Complex; D000110:Acetylcholinesterase