Effects of cathepsin B, H, and D in pectoral muscle of dystrophic chickens (line 413) of in vivo administration of E-64-c (N-[N-(L-3-transcarboxyoxirane-2-carbonyl)-L-leucyl]-3-methyl-butylamine).
The total activities of cathepsin B and cathepsin H in pectoral muscle of dystrophic chickens (Line 413) were about two times higher than in control chickens (Line 412), and cathepsin D activity was about 3 times higher in this muscle in the dystrophic chickens. When E-64-c, a synthesized potent thiol inhibitor was injected subcutaneously, in various doses, daily for 80 days into dystrophic chickens (L 413), the activities of cathepsin B and cathepsin H were reduced to the levels in control chickens (Line 412), but cathepsin D activity, which is insensitive to E-64-c in vitro, was not changed.
D000818:Animals; D002401:Cathepsin B; D002402:Cathepsin D; D056655:Cathepsin H; D002403:Cathepsins / Q000378:metabolism*; D002645:Chickens; D003546:Cysteine Endopeptidases; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009132:Muscles / Q000187:drug effects / Q000201:enzymology*; D009137:Muscular Dystrophy, Animal / Q000201:enzymology*; D011480:Protease Inhibitors / Q000494:pharmacology*
D011480:Protease Inhibitors; C032425:N-(N-(3-carboxyoxirane-2-carbonyl)leucyl)isoamylamine; D002403:Cathepsins; D003546:Cysteine Endopeptidases; D002401:Cathepsin B; D056655:Cathepsin H; D002402:Cathepsin D; D007930:Leucine