The in vitro metabolism of N,N-dimethylaniline by guinea pig and rabbit tissue preparations.
A study of the in vitro metabolism of N,N-dimethylaniline using guinea pig and rabbit preparations and GLC techniques has confirmed N-demethylation and N-oxidation and established ring hydroxylation as metabolic routes. Whereas N-demethylation and N-oxidation are major routes of metabolism, ring hydroxylation is a comparatively minor pathway. Like N-demethylase and N-oxidase, the 4-hydroxylase has been shown to be a microsomal enzyme. The major ring hydroxylated product of N,N-dimethylaniline is N,N-dimethyl-4-aminophenol; N-methyl-4-aminophenol is subsequently formed. The apparent Km and V max values for N-demethylation, N-oxidation and 4-hydroxylation, are presented for both the guinea pig and the rabbit.
D000814:Aniline Compounds / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D006224:Cricetinae; D005289:Ferrets; D006168:Guinea Pigs; D006900:Hydroxylation; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008647:Mesocricetus; D008861:Microsomes / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D014018:Tissue Distribution
D000814:Aniline Compounds; C015157:N,N-dimethylaniline; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; D010089:Oxidoreductases, N-Demethylating