[Oncogen localization in simian adenovirus DNA. I. In vitro rat cell transformation using the SV20 virus and its DNA].
Cell transformation by the complete and the incomplete virus SV20 was investigated. To compare the oncogenic properties of human adenoviruses, two types of cell systems--the primary rat embryonic cell culture and the primary rat kidney cell culture--were used. The dependence of DNA concentration on transformation frequency was investigated. With the incomplete virus SV20, the transformation efficiency was more than that with the complete virus (16 and 1-3 percent, resp.). In addition, it has been shown that the primary rat kidney cell culture is more sensitive to transformation than the primary rat embryonic cells culture. The new-born Sirian hamsters being inoculated with virus SV20 DNA, the growth of a tumor being registered in the point of injection 120 days afterwards.
D000256:Adenoviridae / Q000472:pathogenicity*; D000261:Adenoviruses, Simian / Q000235:genetics / Q000472:pathogenicity*; D000818:Animals; D000831:Animals, Newborn; D002273:Carcinogens; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000494:pharmacology*; D003673:Defective Viruses / Q000235:genetics / Q000472:pathogenicity; D004622:Embryo, Mammalian; D005814:Genes, Viral; D066298:In Vitro Techniques; D007668:Kidney; D051381:Rats; D011919:Rats, Inbred Strains; D014776:Virus Cultivation
D002273:Carcinogens; D004279:DNA, Viral
K voprosu o lokalizatsii onkogena v DNK adenovirusov obez'ian. I. Transformatsiia in vitro kletok krys s pomoshch'iu virusa SV20 i ego DNK.