Mitogenicity and binding properties of beta-galactoside-binding lectin from chick-embryo kidney.
THe beta-galactoside-binding lectin binds to glucosamine, mannosamine and galactosamine in addition to beta-galactoside, as determined by the inhibition of haemagglutination. Haemagglutination is further extended to examine the interaction of the binding sites for hexosamines and beta-galactosides, indicating that the binding of hexosamine and beta-galactoside is competitive. The lectin also shows strong mitogenic activity toward lymphocytes from mouse lymph node, as determined by the stimulation of thymidine incorporation.
D000818:Animals; D001667:Binding, Competitive; D002241:Carbohydrates; D002352:Carrier Proteins / Q000494:pharmacology*; D002642:Chick Embryo; D005697:Galactosides / Q000494:pharmacology*; D037161:Galectins; D006027:Glycosides / Q000494:pharmacology*; D006384:Hemagglutination / Q000187:drug effects; D007668:Kidney / Q000032:analysis / Q000196:embryology; D037102:Lectins / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D002241:Carbohydrates; D002352:Carrier Proteins; D005697:Galactosides; D037161:Galectins; D006027:Glycosides; D037102:Lectins; D013936:Thymidine