Extraction of group A streptococcal M protein with nitrous acid.
Specific precipitating and opsonic neutralizing antigens were identified in dilute-nitrous acid extracts of group A streptococci. This was done by decreasing the temperature and time of exposure of the streptococcal cells to the nitrous acid. Ninety percent of 31 strains of group A streptococcal dilute-nitrous acid extracts formed precipitin lines of identity with conventional Lancefield extracts of the same group A streptococci.
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002352:Carrier Proteins; D008722:Methods; D009500:Neutralization Tests; D009608:Nitrous Acid; D011233:Precipitin Tests; D013696:Temperature; D013997:Time Factors
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C014689:streptococcal M protein; D009608:Nitrous Acid