Tritiation of alpha-bungarotoxin with N-succinimidyl [2,3-3H]propionate. A useful reagent for labelling proteins.
Mono[3H]propionyl-alpha-bungarotoxin, prepared with N-succinimidyl [2,3-3H]propionate (sp. radioactivity 50 Ci/mmol) and purified to homogeneity by electrofocusing, retains its biological activity and stability. Rate constants for its binding to acetylcholine receptor were 4.4-fold lower than for unlabelled toxin; no dissociation was detectable. Analysis of enzymic digests of toxin showed 3H is located mainly of entirely in epsilon-propionyl-lysine.
D002038:Bungarotoxins / Q000302:isolation & purification* / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D007525:Isoelectric Focusing; D007553:Isotope Labeling / Q000379:methods*; D007700:Kinetics; D011422:Propionates / Q000378:metabolism*; D013388:Succinimides / Q000378:metabolism*; D014316:Tritium
D002038:Bungarotoxins; D011422:Propionates; D013388:Succinimides; D014316:Tritium; C021749:N-succinimidyl propionate