Isotachophoresis of urinary purines and pyrimidines: the use of spacers and enzymes for identification.
An isotachophoretic system is described for the separation and identification of urinary purine and pyrimidine bases and nucleosides. For a better discrimination and interpretation of the UV profiles, well-defined non-UV-absorbing substances were introduced as spacers. Treatment of urine samples with purified enzymes before analysis resulted in specific shifts in the metabolite profiles, providing a sensitive and specific means of identifying a number of metabolites. With an injected volume of 3 microliters (untreated urine diluted 1:5) the present method allows reproducible separations within 20 min of at least twenty different nucleosides and bases.
D000493:Allopurinol / Q000627:therapeutic use; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D007926:Lesch-Nyhan Syndrome / Q000188:drug therapy / Q000652:urine; D009705:Nucleosides / Q000652:urine; D011687:Purines / Q000652:urine*; D011743:Pyrimidines / Q000652:urine*
D009705:Nucleosides; D011687:Purines; D011743:Pyrimidines; D000493:Allopurinol