Some cytofluorimetric studies of the nuclear ploidy of mouse hepatocytes. I. A simple method for isolation of hepatocyte nuclei using in situ perfusion of the liver.
A simple method for isolating mouse hepatocyte nuclei is presented, which requires no special apparatus. Flow cytofluorimetric scans of hepatocyte nuclei, prepared by this method and stained with ethidium bromide, showed that adult mice have a high proportion of their hepatocyte nuclei in the tetraploid and octaploid states. Polyploidy was more pronounced in female animals.
D000818:Animals; D002467:Cell Nucleus; D002469:Cell Separation / Q000379:methods; D005260:Female; D005434:Flow Cytometry; D008099:Liver / Q000166:cytology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D011003:Ploidies