title: In vitro microwave effects on human neutrophil precursor cells (CFU-C).
abstract: Human marrow cells were irradiated with 2450-MHz CW microwaves in a fluid-filled waveguide irradiation system. Cell exposure was conducted by placing a marrow cell suspension in 20-microliter glass microcapillary tubes were positioned in the exposure chamber, and irradiated at power densities from 31 to 1,000 mW/cm2 (with corresponding specific absorption rates of 62 to 2,000 mW/g) for 15 minutes. The temperature of the sample was maintained at a fixed point. Sham-irradiated (SI) and microwave-irradiated (MWI) cells were cultured in a methylcellulose culture system for neutrophil colony proliferation. There was no reduction in neutrophil colony number on days 6-7 or 12-14 in cells exposed at 31 or 62 mW/cm2, but as the power density was increased to 1,000 mW/cm2, there was a reduction in colony number of MWI cells compared with SI cells. The microwave interaction with the human neutrophil colony-forming cells was apparently not related to temperature rise, or to the state of cells cycle, and was irreversible.
mesh_terms: D001853:Bone Marrow / Q000528:radiation effects*; D003114:Colony-Forming Units Assay; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D066298:In Vitro Techniques; D007938:Leukemia / Q000097:blood*; D008264:Macrophages / Q000528:radiation effects; D008872:Microwaves; D009504:Neutrophils / Q000528:radiation effects*; D013696:Temperature; D013997:Time Factors