Syringing as a method of cell dispersal. II. Effect on abnormal cells.
The generation of turbulent shear force using a constant pressure syringing device has been demonstrated to be a simple, effective method for the dispersal of intermediate and superficial squamous cells. This paper reports results of an evaluation of the effects of syringing on the dispersal of abnormal cells. Gynecologic cell samples obtained from preinvasive and invasive lesions were syringed. Overall cell loss, as well as degree of dispersion, was evaluated. Doublet, triplet and larger abnormal cell groupings remained in most syringed aliquots regardless of pressure. Although some cell loss was observed in the majority of cases, the percentage of single abnormal cells was increased in 96% of the syringed aliquots.
D000230:Adenocarcinoma / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002469:Cell Separation / Q000379:methods*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D013048:Specimen Handling / Q000379:methods*; D013314:Stress, Mechanical; D013594:Syringes; D002583:Uterine Cervical Neoplasms / Q000473:pathology*; D014594:Uterine Neoplasms / Q000473:pathology*