Stimulation of transfer of methyl groups from O6-methylguanine in DNA to protein by rat liver extracts in response to hepatotoxins.
An enzymatic activity present in rat liver extracts catalyzes the transfer of methyl groups from O6-methylguanine in DNA to protein. This activity was stimulated by treatment of rats with thioacetamide, carbon tetrachloride, 1,2-dimethylhydrazine, diethylnitrosamine, dimethylnitrosamine and by partial hepatectomy but not by treatment with N-methyl-N-nitrosourea or streptozotocin. These results suggest that an enhancement of this activity accompanies the increase in cell division brought about by these agents and is not necessarily a specific response to the presence of alkylated bases in DNA.
D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D008110:Liver Extracts / Q000494:pharmacology*; D008297:Male; D008745:Methylation; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D013853:Thioacetamide / Q000494:pharmacology; D013997:Time Factors
D008110:Liver Extracts; D011506:Proteins; D013853:Thioacetamide; D006147:Guanine; D004247:DNA; C008449:O-(6)-methylguanine; D002251:Carbon Tetrachloride