The chemical structure of neutral and acidic sugar chains obtained from bovine colostrum kappa-casein.
One neutral oligosaccharide alditol (tetrasacchariditol, 10%) and three acidic oligosaccharide alditols (a tri (28%), tetra (17%) and a pentasacchariditol (44%) were isolated from, colostrum kappa-casein taken 6 h after parturition. The neutral tetrasacchariditol and the acidic pentasacchariditol only contained a characteristic N-acetylglucosamine, and the latter was the main carbohydrate portion. The chemical structures were analyzed by methylation analysis with gas-liquid chromatography-mass spectrometry and by anomer analysis with 13C nuclear magnetic resonance, and were identified to be galactosyl-beta-1-3-[galactosyl-beta-1-4-N-acetylglucosaminyl-beta-1-6-]-N-acetylgalactosaminitol, N-acetylneuraminyl-alpha-2-3-galactosyl-beta-1-3-[galactosyl-beta-1-4-N-acetylglucosaminyl-beta-1-6-]-N-acetylgalactosaminitol, N-acetylneuraminyl-alpha-2-6-N-acetylgalactosaminitol. The former two were the novel carbohydrate chains of colostrum kappa-casein, but the latter two were identical with those which have been found in normal kappa-casein.
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002364:Caseins; D002417:Cattle; D003126:Colostrum / Q000032:analysis*; D005260:Female; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry; D008745:Methylation; D009844:Oligosaccharides / Q000032:analysis*
D002241:Carbohydrates; D002364:Caseins; D009844:Oligosaccharides