Halothane modifies colchicine binding to tubulin.
Tubulin extracted from rat brains was incubated at 37 degrees C with 3H-colchicine in the presence and absence of 28 mM halothane. The amount of 3H-colchicine bound to tubulin was reduced by nearly 50% at a colchicine concentration of 5 x 10(-5) M when halothane was present. At low (1 x 10(-7) M) and at high (1 x 10(-3) M) concentrations of colchicine, halothane did not affect the binding as much. The inhibition of colchicine-binding to tubulin by halothane appeared to be non-competitive.
D000818:Animals; D001921:Brain / Q000378:metabolism; D002850:Chromatography, Gel; D003078:Colchicine / Q000378:metabolism*; D006221:Halothane / Q000494:pharmacology*; D007700:Kinetics; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D011919:Rats, Inbred Strains; D014404:Tubulin / Q000378:metabolism*
D014404:Tubulin; D003078:Colchicine; D006221:Halothane