title: Tumour cells in metastatic deposits with altered sensitivity to natural killer cells.
abstract: If natural killer (NK) cells play a role in immunosurveillance it might by expected that, during the metastatic process, selection would occur for tumour cells with reduced NK sensitivity. This hypothesis was tested in the rat by measuring the NK sensitivity of cells freshly isolated from metastases of syngeneic transplanted spontaneous mammary carcinomas. Lysis was measured in a 6-h chromium release assay using normal syngeneic spleen cells as effectors. Our studies led to the following conclusions. (1) Metastases developing at certain tissue sites (draining lymph node and lung, but not pericardium) were frequently composed of tumour cells with markedly reduced sensitivity to NK cells. (2) This resistance could generally be detected only if freshly isolated tumour cell population were studied; after a few days in culture, resistant metastasis-derived tumour cells usually regained normal NK sensitivity. (3) Resistance to NK cells was not always due to the loss of NK target structures; it could also result from an innate resistance to the NK lytic mechanism. (4) The tissue distribution of NK-resistant metastases suggested that if NK cells exerted an immunoselective pressure they did so at the tissue site rather than in the primary tumour or in the bloodstream.
mesh_terms: D000230:Adenocarcinoma / Q000276:immunology*; D000818:Animals; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D005260:Female; D007694:Killer Cells, Natural / Q000276:immunology*; D008207:Lymphatic Metastasis / Q000276:immunology* / Q000473:pathology; D008325:Mammary Neoplasms, Experimental / Q000276:immunology*; D009368:Neoplasm Transplantation; D009369:Neoplasms; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology; D014185:Transplantation, Isogeneic