[Cell culture of normal human epidermis and skin cancer with 3T3 feeders. The establishment of a cancer cell line and its application to the study for the immune response of the autologous patient (author's transl)].
Normal human epidermal cells were cultured with 3T3 feeders, which increased the efficiency of colony formation and promoted the development of colonies. The fact that some colony formation and development were obtained in the cell culture of the adult epidermis, which had been supposed to be difficult, suggests that this technique with 3T3 feeders may be applied to the culture of the diseased epidermis, for instance, dyskeratoses. Another application of 3T3 feeders was made to the culture of skin cancer cells, and a cell line in vitro (HSC-1) was established from a case of skin squamous cell carcinoma. The established cell line was used for the immunological study against the autologous patient who was alive with recurrence of tumor. The immunofluorescent test revealed slightly positive IgG, or C3 on the cell surface of less than 1% of HSC-1 cells. The cellular immunity was negative in the patient against the autologous cell line. However, "Natural Killer"-like cytotoxicity was observed in vitro, both in autologous and allogeneic combinations.
D000328:Adult; D000368:Aged; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D004817:Epidermis / Q000648:ultrastructure*; D006801:Humans; D008297:Male; D051379:Mice; D012878:Skin Neoplasms / Q000276:immunology* / Q000648:ultrastructure
D000951:Antigens, Neoplasm