Isolation and characterization of a mannose/N-acetylglucosamine/fucose-binding protein from rat liver.
A rat liver mannan-binding protein was isolated by affinity chromatography on invertase--Sepharose by a modification of the method of Kawasaki, Etoh & Yamashina [(1978) Biochem. Biophys. Res. Commun. 81, 1018-1024] and by a new method involving chromatography on mannose-Sepharose. The binding protein appears as a single band on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis with an apparent mol.wt. of approx. 30000. Binding of 125I-labelled mannan is saturable and inhibited by mannose, N-acetylglucosamine, or L-fucose but not by galactose or mannose 6-phosphate. Neoglycoproteins containing mannose, N-acetylglucosamine, or L-fucose, but not galactose, are inhibitory. The neoglycoproteins are 10000-fold more effective (based on moles of sugar) than are free monosaccharides as inhibitors. 125I-labelled mannan binding to the binding protein is calcium-dependent.
D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D037222:Collectins; D004591:Electrophoresis, Polyacrylamide Gel; D005643:Fucose / Q000378:metabolism*; D005944:Glucosamine / Q000031:analogs & derivatives*; D006023:Glycoproteins / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008351:Mannans / Q000302:isolation & purification / Q000378:metabolism*; D009005:Monosaccharides / Q000494:pharmacology; D011134:Polysaccharides / Q000378:metabolism*; D051381:Rats
D002352:Carrier Proteins; D037222:Collectins; D006023:Glycoproteins; D008351:Mannans; D009005:Monosaccharides; D011134:Polysaccharides; D005643:Fucose; D005944:Glucosamine; D002118:Calcium; D000117:Acetylglucosamine