Monitoring induction of ovulation by rapid radioimmunoassays of oestrogen and pregnanediol glucuronides.
To increase the sample-handling capacity for an induction of ovulation programme, direct urinary radioimmunoassays (RIA) for three steroid glucuronides, pregnanediol-3-glucuronide (Pd-3-G), oestrone-3-glucuronide (E1-3-G), and oestriol-16 alpha-glucuronide (E3-16-G), were established. Results obtained for urinary Pd-3-G measured by direct RIA show an excellent correlation (r = 0.98, N = 46) with those for urinary pregnanediol measured by gas-liquid chromatography. Estrone-3-glucuronide (E1-3-G) and estriol-16 alpha-glucuronide (E3-16-G) values, measured by direct RIA, closely paralleled the total urinary oestrogen measured fluorimetrically. Ovarian response to ovulation induction therapy can be monitored by observing the changes in the levels of E1-3-G in urine. Pre-ovulatory levels of urinary E3-16-G were found to be too low for use in this regard. Direct RIA for E1-3-G and Pd-3-G are recommended as reliable indices of ovarian function in the monitoring of patients receiving treatment for the induction of ovulation.
D000818:Animals; D004964:Estriol / Q000031:analogs & derivatives* / Q000652:urine; D004970:Estrone / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D005965:Glucuronates / Q000652:urine; D006801:Humans; D007247:Infertility, Female / Q000188:drug therapy / Q000652:urine; D010062:Ovulation Induction; D010902:Pituitary Gland / Q000032:analysis; D011276:Pregnanediol / Q000031:analogs & derivatives* / Q000652:urine; D011817:Rabbits; D014020:Tissue Extracts / Q000627:therapeutic use
D005965:Glucuronates; D014020:Tissue Extracts; C022270:pregnanediol-3 alpha-glucuronide; C012961:estriol-16 alpha-(beta-D-glucuronide); C015856:estrone-3-glucuronide; D004970:Estrone; D004964:Estriol; D011276:Pregnanediol