Relationship of carbon tetrachloride-induced alterations of ligandin and Z protein to sulfobromophthalein transport in rat liver.
The relationship of the BSP retaining capacity of organic anion binding protein in the liver to the transport of BSP from plasma to bile was examined in CCl4-treated rats. These proteins were separated into ligandin and Z protein by Sephadex G-75 gel chromatography. The BSP retaining capacity of these proteins was significantly decreased by CCl4 administration (2.5 ml/kg, p.o.). At 3 hours after CCl4, this decreased capacity was caused mainly by the decrease in their binding affinity, at 24 hours after CCl4, however, it was accompanied by decreases in both their binding affinity and protein amounts. At 3 hours, the hepatic uptake of BSP evaluated by determination of plasma BSP concentration, the initial disappearance rate (K1), and the hepatic dye content did not differ from those in the controls, and biliary excretion of BSP was unchanged. At 24 hours, hepatic uptake and biliary excretion of BSP as well as bile flow were significantly decreased. From these results, it is suggested that the alteration in the retaining capacity of ligandin and Z protein would not be a determinant factor in the transfer of BSP from plasma to bile.
D000818:Animals; D001646:Bile / Q000378:metabolism; D001663:Bilirubin / Q000097:blood; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002352:Carrier Proteins / Q000032:analysis* / Q000502:physiology; D050556:Fatty Acid-Binding Proteins; D005982:Glutathione Transferase / Q000032:analysis* / Q000502:physiology; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D013448:Sulfobromophthalein / Q000378:metabolism*
D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D013448:Sulfobromophthalein; D002251:Carbon Tetrachloride; D005982:Glutathione Transferase; D001663:Bilirubin