The apparent inhibition of urothelial DNA synthesis in neonatal rats by dietary saccharin.
[3H]Thymidine ([3H]TdR) incorporation into urothelial DNA of male neonatal rats was measured autoradiographically at birth and during the first 3 weeks of life. The rats were derived from control parents and those fed saccharin (1, 3, 5 and 7.5%) in the diet from before pregnancy. [3H]TdR incorporation was inhibited and there were more lightly labeled cells (compared with controls), in all the saccharin-exposed rats in a rough dose-dependent manner. The results, in comparison with controls, suggest that saccharin exposure in utero causes DNA damage in the neonatal urothelium manifesting as reduced thymidine incorporation and a greater proportion of lightly labeled cells.
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000037:antagonists & inhibitors*; D004032:Diet; D005260:Female; D005333:Fetus / Q000187:drug effects; D008297:Male; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D051381:Rats; D011919:Rats, Inbred Strains; D012439:Saccharin / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014566:Urogenital System / Q000187:drug effects / Q000196:embryology*
D014316:Tritium; D004247:DNA; D012439:Saccharin; D013936:Thymidine