Biochemistry of glomerular basement membrane components purified by nondisruptive techniques.
In contrast to the fragmentation and loss of spatial arrangement seen in preparations of basement membrane isolated after sonic disruption, the architectural integrity of glomerular basement membrane is presented with the described technique of osmotic lysis and sequential detergent treatment. The biochemistry of components solubilized without pepsin digestion from GBM prepared with this technique is described.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000032:analysis*; D002850:Chromatography, Gel; D003094:Collagen / Q000032:analysis; D003902:Detergents; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D007678:Kidney Glomerulus / Q000032:analysis*; D008722:Methods; D008970:Molecular Weight; D011347:Procollagen / Q000032:analysis; D051381:Rats
D000596:Amino Acids; D003902:Detergents; D011347:Procollagen; D003094:Collagen