Influence of bioflavonoids on the metabolism and crosslinking of collagen.
The influence of (+)-catechin (10 mg/100 g daily) and 0-(beta-hydroxyethyl) rutosides (50 mg/100 g daily) on the metabolism and crosslinking of collagen was studied in male albino rats. Compared to controls, the total activity of 3H-hydroxyproline in the dermal soluble collagen did not differ appreciably while the urinary total activity of 3H-hydroxyproline was significantly lowered. When the total activities of both soluble and insoluble collagens are expressed as a percentage of the sum of both, the registered activity was less in soluble and more in salt-insoluble collagen at 12 ans 120 h after the administration of 3H-proline in the treated group. Bioflavonoids were found to decrease the reversibility of neutral salt-soluble collagen gel and solubility of acid-insoluble collagen to denaturing agents. The subunit pattern of neutral salt-soluble collagen on sodium dodecylsulphate polyacrylamide gels revealed a decrease of alpha/beta ratio in bioflavonoids treated animals. The results of the present study indicate that the synthesis of collagen is unaffected, the crosslinking of collagen is promoted and the degradation of soluble collagen is decreased in the bioflavonoids treated groups.
D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D002392:Catechin / Q000494:pharmacology*; D003094:Collagen / Q000096:biosynthesis / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006896:Hydroxyethylrutoside / Q000494:pharmacology*; D046911:Macromolecular Substances; D008297:Male; D051381:Rats; D012431:Rutin / Q000031:analogs & derivatives*; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D012995:Solubility
D001578:Benzopyrans; D006896:Hydroxyethylrutoside; D046911:Macromolecular Substances; D012431:Rutin; D002392:Catechin; D003094:Collagen