[Alpha-tocopherol and actinomycin D regulation of ubiquinone and protein biosynthesis in rat liver in E-hypovitaminosis].
In vitro experiments with the liver preparations from rats with E-hypovitaminosis revealed a more intensive incorporation of [14C]phenylalanine into ubiquinone induced by alpha-tocopherol. Actinomycin D inhibiting the DNA-dependent RNA synthesis decreases the incorporation intensity. A 30 min preincubation of the liver preparations with thesis. In case of preliminary addition of actinomycin D or its simultaneous introduction with alpha-tocopherol the latter has no stimulating effect on the intensity of [14C] phenylalanine incorporation into ubiquinone. When studying incorporation of [14C]phenylalanine into the total protein of the liver of rats with E-hypovitaminosis a tendency is revealed to an increase in the protein biosynthesis intensity in case of preincubation with alpha-tocopherol and to inhibition of the labelled precursor incorporation under conditions of preincubation with actinomycin D. The data obtained permit suggesting that the alpha-tocopherol effect on the ubiquinone biosynthesis may occur due to its effect on the RNA synthesis.
D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects*; D051381:Rats; D014451:Ubiquinone / Q000096:biosynthesis*; D014810:Vitamin E / Q000494:pharmacology*; D014811:Vitamin E Deficiency / Q000378:metabolism*
D014451:Ubiquinone; D014810:Vitamin E; D003609:Dactinomycin
Reguliatsiia al'fa-tokoferolom i aktinomitsinom D biosinteza ubikhinona i belka v pecheni krys pri E-gipovitaminoze.