A dual laser flow sorter utilizing a CW pumped dye laser.
A dual laser flow sorter has been constructed from an existing single laser system by incorporating a dye laser as the second laser source. The argon ion laser emission was used both as a pump laser and as a source by beam splitting before entrance to the dye laser. The emissions of the dye laser and pump laser beams were recombined and focused with the same optical train used in the single laser system. The imaging on the stream of the flow sorter provided for spatial and thus temporal separation of the exciting beams and subsequent sample emissions.
D002469:Cell Separation / Q000295:instrumentation*; D005434:Flow Cytometry / Q000295:instrumentation*; D005456:Fluorescent Dyes; D007834:Lasers
D005456:Fluorescent Dyes