title: Regulation of erythroid cell differentiation by haemin.
abstract: The differentiation of immature erythroblasts, isolated from anaemic rabbit bone marrow by density centrifugation to bovine serum albumin gradients, is accelerated by the addition of 10(-5)-10(-4) M haemin to the culture medium. Both the proportion of benzidine-positive cells and the synthesis of haemoglobin relative to the total protein were increased, whereas cell growth and DNA synthesis were decreased. Some of these changes were detected within 4 h and were maximal after 18-40 h. It is suggested that haem may have a physiological role in regulating in vivo erythropoiesis during haemolysis by accelerating terminal erythroid cell differentiation.
mesh_terms: D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D004900:Erythroblasts / Q000166:cytology* / Q000378:metabolism; D004912:Erythrocytes / Q000166:cytology*; D004920:Erythropoiesis / Q000187:drug effects*; D006418:Heme / Q000031:analogs & derivatives*; D006427:Hemin / Q000494:pharmacology*; D006454:Hemoglobins / Q000096:biosynthesis; D006918:Hydroxyurea / Q000494:pharmacology; D011817:Rabbits
chemical_list: D000546:Amanitins; D006454:Hemoglobins; D006418:Heme; D006427:Hemin; D004247:DNA; D006918:Hydroxyurea