title: Increased muscle calcium. A possible cause of mitochondrial dysfunction and cellular necrosis in denervated rat skeletal muscle.
abstract: Mitochondrial preparations derived from denervated rat skeletal muscle and paired controls were characterized with respect to their ability to take up externally added Ca2+. The denervated and control muscle homogenates and mitochondrial [Ca2+] were also determined. Our data indicate that the denervated mitochondria are able to take up less Ca2+ than the controls before uncoupling occurs. This defect is associated with elevated [Ca2+] in homogenate and mitochondrial fractions in the denervated state. The causal relationship between Ca2+ overload, mitochondrial functional damage and cell necrosis is discussed.
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D008931:Mitochondria, Muscle / Q000378:metabolism*; D009121:Muscle Denervation; D009132:Muscles / Q000378:metabolism* / Q000473:pathology; D009336:Necrosis; D010101:Oxygen Consumption; D051381:Rats
chemical_list: D002118:Calcium