Specificity of deoxycytidylate antibodies.
Antibodies to deoxycytidylate (dpC) were elicited in rabbits using a thyroglobulin (Tg) conjugate of dpC. The specificity of the antibodies was determined by measuring the inhibition of the binding of [3H]dpC to the antibodies by various non-radioactive nucleotides or derivatives. The antibodies were found to distinguish dpC from pC and DNA from RNA, probably due to their specificity for the conformation of the deoxycytidylate.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001667:Binding, Competitive; D003596:Cytosine / Q000494:pharmacology; D003597:Cytosine Nucleotides / Q000494:pharmacology; D003838:Deoxyadenine Nucleotides / Q000494:pharmacology; D003841:Deoxycytidine / Q000031:analogs & derivatives / Q000494:pharmacology; D003845:Deoxycytosine Nucleotides / Q000276:immunology* / Q000494:pharmacology; D003856:Deoxyuracil Nucleotides / Q000494:pharmacology; D011817:Rabbits; D013942:Thymine Nucleotides / Q000494:pharmacology
D003597:Cytosine Nucleotides; D003838:Deoxyadenine Nucleotides; D003845:Deoxycytosine Nucleotides; D003856:Deoxyuracil Nucleotides; D013942:Thymine Nucleotides; D003841:Deoxycytidine; D003596:Cytosine; C016569:5-methyldeoxycytidine