Computer programming for nucleic acid studies. III. Calculated ultraviolet absorption spectra of protected oligodeoxyribonucleotides.
A computer program called UV. FOR was written in FORTRAN. This program primarily utilizes the digitized UV absorption spectra of 8 protected deoxyribonucleosides in 95% ethanol solution to compose the UV spectrum of a oligodeoxynucleotide of any sequence. Both calculated and observed UV spectra of 2 protected oligodeoxynucleotides are carefully compared. The results show that the calculated UV spectrum is virtually identical to the observed spectrum. Thus, the calculated spectra provide rapid confirmation of oligonucleotide compositions during the course of oligonucleotide synthesis by the phosphotriester method.
D003201:Computers; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D013054:Spectrophotometry, Atomic / Q000379:methods*; D014466:Ultraviolet Rays
D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides