[Limitations in the translation of influenza virus mRNAs in infected cells].
The monolayer cultures of chicken embryonic cells were infected with influenza virus and 6 hrs after the infection the relative concentrations of viral mRNAs and the relative rates of virus-specific protein synthesis were determined. For this purpose the infected cells were incubated under conditions of continuous labelling of RNAs by the radioactive precursor; the mRNA fraction was obtained by hybridization of total RNA of infected cells with non-labelled RNA of viral particles and the individual mRNAs were fractionated by polyacrylamide gel electrophoresis of RNA duplexes. The proteins from infected cells were labelled by [14C] amino acids for a short time and the individual virus-specific proteins were separated electrophoretically. There was no correlation between the rates of virus-specific protein synthesis and the relative concentrations of corresponding mRNAs. This indicates the regulation of virus-specific protein synthesis at the post-translational level.
D000818:Animals; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D009980:Influenza A virus / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
D012333:RNA, Messenger; D014764:Viral Proteins
Ogranicheniia transliatsii informatsionnykh RNK virusa grippa v zarazhennykh kletkakh.