Determination of the Structure of four glycopeptides from hen ovalbumin using 360-MHz proton magnetic resonance spectroscopy.
Four glycopeptides have been purified by Dowex and Bio-Gel P2 chromatography from Pronase digests of hen ovalbumin. The high-resolution proton magnetic resonance spectra of these glycopeptides and various products of their enzymatic digestion have been obtained at 360 MHz. By use of information derived from the spectra of a number of model compounds, an unambiguous assignment of all C1-H and Man C2-H resonances in the spectra can be made. On this basis structures are proposed for the four glycopeptides which are identical with those structures previously deduced from destructive chemical methods.
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002645:Chickens; D005260:Female; D006020:Glycopeptides; D006026:Glycoside Hydrolases; D006601:Hexoses / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000032:analysis; D010047:Ovalbumin
D006020:Glycopeptides; D006601:Hexoses; D009844:Oligosaccharides; D010047:Ovalbumin; D006026:Glycoside Hydrolases