Flurbiprofen, a non-steroid anti-inflammatory agent, protects cells against hypoxic cell radiosensitizers in vitro.
Overnight exposure of Chinese hamster cells (V79-753B) to 5 x 10(-5) M flurbiprofen (2-(2-fluoro-4-biphenyl)propionic acid) in vitro reduced the cytotoxic effects of misonidazole, 1-methyl-4-nitro-5-phenoxysulphonylimidazole (NSC 38087) and nitrofurantoin, both in air and in hypoxia at 37 degrees C. Flurbiprofen did not alter the cells' uptake of 14C-misonidazole, nor did it affect the radiosensitivity of aerobic or anaerobic cells, or the degree of hypoxic-cell radiosensitization produced by the sensitizers. When flurbiprofen-treated cells were exposed to melphalan there was no protection against cytotoxicity. These data suggest that flurbiprofen may inhibit the catabolism of radiosensitizers to toxic products and indicate the need to examine whether it will protect against misonidazole-induced toxicity in vivo.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D005480:Flurbiprofen / Q000494:pharmacology*; D008920:Misonidazole / Q000037:antagonists & inhibitors*; D009582:Nitrofurantoin / Q000037:antagonists & inhibitors*; D009593:Nitroimidazoles / Q000037:antagonists & inhibitors*; D010100:Oxygen; D011422:Propionates / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000037:antagonists & inhibitors*
D009593:Nitroimidazoles; D011422:Propionates; D011838:Radiation-Sensitizing Agents; D005480:Flurbiprofen; C031761:5-phenoxysulfonyl-1-methyl-4-nitroimidazole; D008920:Misonidazole; D009582:Nitrofurantoin; D010100:Oxygen