Effects of hypotonic treatment on human metaphase chromosome length.
The effects of hypotonic treatment on the structure and length of chromosomes were studied using cell cultures from human blood and skin. A strong correlation of chromosomal prolongation following treatment with solutions of increasing hypotonicity was demonstrated. This may be a suitable tool to prepare chromosomes for banding technique.
D002478:Cells, Cultured; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure*; D005260:Female; D005347:Fibroblasts; D006801:Humans; D007038:Hypotonic Solutions / Q000494:pharmacology*; D008214:Lymphocytes; D008297:Male; D011189:Potassium Chloride / Q000494:pharmacology
D007038:Hypotonic Solutions; D011189:Potassium Chloride