A comparison of the accumulation of ricin by hepatic parenchymal and non-parenchymal cells and its inhibition of protein synthesis.
Rat liver non-parenchymal cells in vivo were found to accumulate 125I-labelled ricin to a much greater extent than parenchymal cells. Similarly, in monolayer cell cultures, the rate of ricin uptake by non-parenchymal Kupffer cells was several times that by parenchymal cells. Evidence is provided also to suggest that ricin is primarily recognized by Kupffer cells via terminal mannose residues in the toxin, whereas ricin uptake by parenchymal cells was consistent with a role of the previously postulated galactosyl-containing cell receptors. Protein synthesis in Kupffer cells in vitro, although observed to occur at a lower rate than in parenchymal cells, was 100--1000-times more sensitive to inhibition by ricin. The selective damage known to be caused to liver sinusoids by ricin, therefore, may reflect both the relative efficiency with which the toxin is taken up by these cells and the extreme sensitivity of protein synthesis in the cells to inhibition by ricin.
D000818:Animals; D002478:Cells, Cultured; D005690:Galactose / Q000494:pharmacology; D007728:Kupffer Cells / Q000378:metabolism; D007785:Lactose / Q000494:pharmacology; D008099:Liver / Q000378:metabolism*; D008297:Male; D008358:Mannose / Q000494:pharmacology; D014176:Protein Biosynthesis; D051381:Rats; D011919:Rats, Inbred Strains; D012276:Ricin / Q000378:metabolism*
D012276:Ricin; D007785:Lactose; D008358:Mannose; D005690:Galactose