Gluconeogenesis in isolated chicken hepatocytes: effect of fatty acids, beta-hydroxybutrate, ethanol, and various pyruvate/lactate ratios.
The effect of fatty acids beta-hydroxybutyrate, ethanol, and different pyruvate/lactate ratios on gluconeogenesis in isolated chicken hepatocytes was investigated. Glucogenesis was significantly affected by a change in the oxidation-reduction (pyruvate/lactate) ratio, and this effect was greater than could be accounted for by the additive effects of these substrates. Substituting lactate with nongluconeogenic substrates, such as beta-hydroxybutyrate or ethanol, increased the formation of glucose by 80 and 200%, respectively, demonstrating the beneficial effect of the increased reducing equivalents in the hepatocytes. Oleic acid per se had no effect but when added complexed with albumin, it had a negative effect on gluconeogenesis.
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005943:Gluconeogenesis; D006885:Hydroxybutyrates / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism
D005227:Fatty Acids; D006885:Hydroxybutyrates; D007773:Lactates; D011773:Pyruvates; D000431:Ethanol