Simultaneous determination of misonidazole and desmethylmisonidazole in plasma by high-performance liquid chromatography.
A high-performance liquid chromatographic method for the simultaneous determination of misonidazole and desmethylmisonidazole in plasma is described. After plasma is deproteinized with methanol and the diluted supernatant is chromatographed on a C18 reversed-phase column, both compounds are quantitated by means of an internal standard. The coefficients of variation of within-day and day-to-day precision are below 5.0% for misonidazole in the concentration range of 25-250 mg/l and below 6.1% for desmethylmisonidazole in the concentration range of 2.5-25.0 mg/l. Calibration curves are linear and an analytical recovery varying from 97.6 to 99.8% is obtained. The detection limits for misonidazole and desmethylmisonidazole in plasma are 1.4 mg/l and 0.7 mg/l, respectively.
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008920:Misonidazole / Q000031:analogs & derivatives / Q000097:blood*; D009593:Nitroimidazoles / Q000097:blood*; D011878:Radiotherapy / Q000379:methods; D012016:Reference Values
D009593:Nitroimidazoles; D008920:Misonidazole; C009997:desmethylmisonidazole