[Effect of anticoagulants on lymphocyte isolation by density gradient centrifugation].
The authors compared the efficiency of isolation and the purity of lymphocytes obtained by the method of centrifugation in a gradient of ficoll and uropoline from blood obtained on ACD fluid and on heparin. Isolation of lymphocytes from the blood obtained on heparin was more efficient (60%) and useful. For removal of platelets it was necessary to use additional centrifugation in a saccharose gradient or application of aggregating agents during isolation of lymphocytes obtained on heparin or ACD fluid.
D000925:Anticoagulants; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000379:methods*; D002951:Citrates / Q000494:pharmacology*; D019343:Citric Acid; D005947:Glucose / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D012996:Solutions
D000925:Anticoagulants; D002951:Citrates; D012996:Solutions; D019343:Citric Acid; D006493:Heparin; D005947:Glucose
Wpływ czynników antykoagulacyjnych na izolacje limfocytów technika wirowania w gradiencie gestości.