Use of hydroxyurea in the measurement of DNA repair by the BND cellulose method.
Hydroxyurea inhibition is a convenient method of suppressing replicative DNA synthesis for DNA excision-repair measurement by the BND cellulose technique. Nonetheless, hydroxyurea can introduce artefacts by direct reaction with repair-inducing compounds and by long-term inhibition of the overall repair process. A simple technique of overcoming these problems is described. Cells are reacted with repair-inducing compounds in the absence of hydroxyurea, the cells are washed free of inducer, hydroxyurea is added to 2 mM, and after a short period to establish replication inhibition, 3H dThd is added and repair measured over a one-hour incubation period.
D000099:Acetoxyacetylaminofluorene / Q000494:pharmacology; D002460:Cell Line; D002848:Chromatography, DEAE-Cellulose; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology*; D008214:Lymphocytes; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology
D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D000099:Acetoxyacetylaminofluorene; D004247:DNA; D008741:Methyl Methanesulfonate; D006918:Hydroxyurea