Circulatory isolation of the liver for studying energy metabolism in trauma and sepsis.
An isolated level model was used in 25-30 kg pigs to study the uptake and/or release of various energy substrates across the liver and splanchnic bed during trauma and sepsis. This was accomplished by bypassing the hepatic portion of the inferior vena cava (IVC) using a polytetrafluoroethylene (PTFE) (IMPRA, Inc.) graphite-impregnated graft, from above the renal veins to the right atrial junction. The IVC was then ligated between the distal anastomosis and the liver. Sampling was done from inlying catheters placed in the main portal vein, hepatic vena cava, and an artery. Total hepatic blood flow was determined by the dye dilution technique. Our data showed the same pattern of metabolic derangements in both the traumatized and the septic-starved animals, but differed significantly from the normal fed controls. The correlation between glucogenesis and other energy functions of the liver and the proteolysis in skeletal muscle seen in trauma and sepsis is demonstrated. This model is functional and is applicable to the study of various kinds of shock and/or liver failure.
D000596:Amino Acids / Q000097:blood; D000818:Animals; D001424:Bacterial Infections / Q000097:blood / Q000378:metabolism*; D001786:Blood Glucose / Q000032:analysis; D004734:Energy Metabolism; D007773:Lactates / Q000097:blood; D008099:Liver / Q000378:metabolism*; D008102:Liver Circulation; D013152:Splanchnic Circulation; D013552:Swine; D014947:Wounds and Injuries / Q000097:blood / Q000378:metabolism*
D000596:Amino Acids; D001786:Blood Glucose; D007773:Lactates