Hormonal effects on the sulfation of sulfated glycoconjugates in the uterine endometrium of non-ovariectomized rabbit.
A particulate fraction was separated from endometrial scrapings of uterus of hormone-treated non-ovariectomized or intact rabbit. The effects of ovarial hormones on the incorporation of [35S]sulfate from 3'-[35S]phosphoadenosine 5'-phosphosulfate (PAPs) into endogenous acceptors in the particulate fractions were studied. Treatment of the animal with estrogen elevated the incorporation of [35S]sulfate, but exogenous progesterone suppressed this effect. The results of DEAE-Sephadex A-25 (Cl- form) column chromatography of the pronase digest of the 35S-labeled substances indicated that all the subfractions were sensitive to the hormones. The present observations, together with previous findings, suggest that the sulfation of sulfated glycoconjugates in the particulate fractions is stimulated by exogenous estrogen, but exogenous progesterone suppressed this effect, and also that endogenous ovarial hormones exert little influence on the effects of the present doses of these exogenous hormones on the sulfation. Therefore, non-ovariectomized rabbit can be used for rough evaluation of the effects of regular doses of these exogenous hormones on the sulfation of sulfated glycoconjugates in the uterine endometrium.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000818:Animals; D002369:Castration; D051152:Clusterin; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis*; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D007700:Kinetics; D018832:Molecular Chaperones; D010724:Phosphoadenosine Phosphosulfate / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D011817:Rabbits; D013462:Sulfur Radioisotopes
D000227:Adenine Nucleotides; D051152:Clusterin; D006023:Glycoproteins; D006025:Glycosaminoglycans; D018832:Molecular Chaperones; D013462:Sulfur Radioisotopes; C013786:A73025; D010724:Phosphoadenosine Phosphosulfate; D011374:Progesterone; D004958:Estradiol