Quantitative determination of the cholinesterase inhibitor physostigmine in brain tissue samples using reversed-phase high-performance liquid chromatography.
A high-performance liquid chromatographic method, based on a dynamic cation-exchange system was used for the determination of physostigmine in brain tissue extracts. The precision and detection limit of the method as well as the extraction efficiency were established. The distribution of physostigmine over several parts of the brain after intravertebral application is reported.
D000818:Animals; D001921:Brain / Q000201:enzymology; D001923:Brain Chemistry; D002415:Cats; D002800:Cholinesterase Inhibitors / Q000008:administration & dosage / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007275:Injections, Intravenous; D008297:Male; D010830:Physostigmine / Q000008:administration & dosage / Q000032:analysis*
D002800:Cholinesterase Inhibitors; D010830:Physostigmine