Phosphoenolpyruvate-dependent sugar phosphotransferase activity in Megasphaera elsdenii.
Megasphaera elsdenii was found to possess an inducible phosphoenolpyruvate:sugar phosphotransferase system for glucose and fructose but not for other hexoses, hexosamines, or hexitols. The complexity of the Megasphaera phosphoenolpyruvate:sugar phosphotransferase system lies intermediate to systems found in photoautotrophic bacteria and enteric bacteria. Megasphaera elsdenii phosphotransferase proteins exhibited enzymatic cross-reactivity with those from Escherichia coli; however, differences were found in substrate specificities and the physical characteristics of the proteins from these organisms. Sugar uptake was reduced in M. elsdenii stationary-phase as compared with log-phase cells and this loss correlated with a reduction in enzyme II function.
D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism*; D013379:Substrate Specificity; D045854:Veillonellaceae / Q000201:enzymology* / Q000254:growth & development
D005632:Fructose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D005947:Glucose