Immunogenetic studies of H-2 antigens of MCG 3 tumour cells.
MCG 3 is a B 10(H-2b) sarcoma induced as a solid tumour with 0.2 mg of methylcholantrene in our laboratory. The ascites form obtained was used to study the expression of H-2 antigeneic specificities in a postlabelling radioassay. H-2D.2, H-2K.33 (private specificities of H-2b) and H-2.28, H-2.35 (public specificities) were normally expressed for MCG 3 while H-2.5, H-2.29, H-2.36 (public specificities) were abnormally absent. These results were confirmed by quantitative absorption analysis using MCG 3 and positive-negative normal lymphoid cells for a particular specificity.
D000818:Animals; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D006183:H-2 Antigens / Q000276:immunology*; D051379:Mice; D012513:Sarcoma, Experimental / Q000276:immunology*
D006183:H-2 Antigens