title: High-performance liquid chromatographic separation, isolation and identification of 1,2,3-thiadiazole-5-carboxaldoxime glucuronide in rabbit urine.
abstract: Reversed-phase high-performance liquid chromatography was used to separate and isolate the glucuronic acid conjugate of 1,2,3-thiadiazole-5-carboxaldoxime from urine of rabbits after intravenous injection of the oxime. The conjugate was identified by gas chromatography-mass spectrometry  as its trimethylsilylated methyl ester and by nuclear magnetic resonance spectrometry. Additional information was obtained from thin-layer chromatography and high-voltage paper electrophoresis.
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004353:Drug Evaluation, Preclinical; D008401:Gas Chromatography-Mass Spectrometry; D007275:Injections, Intravenous; D009682:Magnetic Resonance Spectroscopy; D011817:Rabbits; D013830:Thiadiazoles / Q000008:administration & dosage / Q000302:isolation & purification / Q000378:metabolism / Q000652:urine*
chemical_list: D013830:Thiadiazoles; C033416:1,2,3-thiadiazole-5-carboxaldoxime; C033417:1,2,3-thiadiazole-5-carboxaldoxime glucuronide