Prostaglandin biosynthesis and lipolysis in subcellular fractions from rabbit kidney medulla.
Three separate prostaglandin-generating activities are associated with plasma membranes, mitochondria and microsomal fractions from rabbit kidney medulla. In the plasma membranes and mitochondria, but not in microsomal fractions, Ca2+ ions stimulate the activity of phospholipase A2, yielding selective release of arachidonic acid and linoleic acid and concomitant increase in prostaglandin E2 formation.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000378:metabolism*; D008066:Lipolysis; D008297:Male; D011453:Prostaglandins / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000096:biosynthesis; D011817:Rabbits; D013347:Subcellular Fractions / Q000378:metabolism
D001095:Arachidonic Acids; D011453:Prostaglandins; D011458:Prostaglandins E; D002118:Calcium