Ultracentrifugation in polyethylene tubing: an efficient method to concentrate protein solutions on the microliter scale.
An ultracentrifugation procedure is described to concentrate protein solutions on the microliter scale. Protein solutions were centrifuged in U-shaped lengths of polyethylene tubing at 160000 X g for 15 h and thereafter fractionated by cutting the tubing. The method can be performed in a conventional ultracentrifuge and needs no special equipment.
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification; D002556:Cerebrospinal Fluid Proteins / Q000302:isolation & purification*; D006801:Humans; D008832:Microchemistry; D011095:Polyethylenes; D011506:Proteins / Q000302:isolation & purification*; D014461:Ultracentrifugation / Q000379:methods
D001798:Blood Proteins; D002556:Cerebrospinal Fluid Proteins; D011095:Polyethylenes; D011506:Proteins