title: GC and GC-mass spectrometric determination of p-hydroxyphenobarbital extracted from plasma, urine, and hepatic microsomes.
abstract: Analytical methodology was developed for the quantitation of p-hydroxyphenobarbital extracted from plasma, urine, and hepatic microsomes. p-Hydroxyphenobarbital was derivatized with an appropriate n-alkyl iodide in the presence of a methanolic base in aprotic solvent medium. The peralkylated derivatives were stable indefinitely and were quantitated by the sensitive and selective method of GC nitrogen-selective detection and/or selected ion monitoring. The accuracy, precision, and cross verification of all methods were good. The analysis was subsequently used to study the effects of other drugs on phenobarbital biodisposition.
mesh_terms: D000818:Animals; D001711:Biotransformation; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000032:analysis*; D010634:Phenobarbital / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood / Q000652:urine; D011324:Primidone / Q000032:analysis; D051381:Rats
chemical_list: D011324:Primidone; C017414:4-hydroxyphenobarbital; D010634:Phenobarbital