Two conserved sequence blocks within eukaryotic tRNA genes are major promoter elements.
The split promoter sequences of a tRNALeuCUG gene of Xenopus laevis have been mapped to nucleotides 13-20 and 51-64 of the tRNALeu coding sequences. The sequences closely coincide with two conserved sequence blocks present in all eukaryotic tRNA genes. The two conserved sequence blocks were found to be exchangeable between tRNA genes as chimaeric tRNAMet--tRNALeu genes proved transcriptionally active. Furthermore, two prokaryotic tRNA genes exhibiting strong homologies with the two blocks yielded specific transcripts when tested in an eukaryotic transcriptional system.
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D005075:Biological Evolution; D005796:Genes; D007930:Leucine; D008715:Methionine; D009876:Operon; D012320:RNA Polymerase III / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics*; D014158:Transcription, Genetic; D014982:Xenopus laevis
D012343:RNA, Transfer; D008715:Methionine; D012320:RNA Polymerase III; D007930:Leucine