Improved viscometric assay for cellulase.
A viscometric assay for the determination of carboxymethylcellulase activity is described. The method, which is developed from a direct theoretical approach, takes into account a kinetic energy correction and the non-Newtonian behaviour of carboxymethylcellulose solutions. The enzymic hydrolysis of carboxymethylcellulose shows strong competitive inhibition by the products of the reaction and a method is presented for estimating the initial velocity of such reactions. A direct chemical determination of reducing end groups was used to confirm the validity of this approach.
D002266:Carboxymethylcellulose Sodium; D002480:Cellulase / Q000378:metabolism*; D007700:Kinetics; D008722:Methods; D014783:Viscosity
D002480:Cellulase; D002266:Carboxymethylcellulose Sodium