Binding of [methyl-3H]paraquat to rat, rabbit, hamster, mouse and guinea pig lung proteins, in vitro.
When incubated with the rat lung slices [methyl-3H]paraquat was found to bind covalently to acid-insoluble proteins. The evidence in the present study indicated that the binding of radioactivity was not due to the formation of a reactive metabolite secondary to mixed-function oxidase (MFO)-mediated bioactivation of paraquat but was probably due to formation of paraquat free radicals. Preincubation of lung slices with CN, 2,4-dinitrophenol (DPN), p-hydroxymercuribenzoate and cysteine inhibited binding, while anaerobic conditions has a marked stimulatory effect. There were no significant differences in the extent of covalent binding of [methyl-3H]paraquat to lung protein of rat. rabbit, mouse, guinea pig, and hamster.
D000693:Anaerobiosis; D000818:Animals; D006224:Cricetinae; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D051379:Mice; D010269:Paraquat / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013997:Time Factors; D014316:Tritium
D014316:Tritium; D010269:Paraquat