[Distribution of lactate dehydrogenase activity in the cerebral cortex in healthy subjects and after a single dose of aminazine (histochemical and electron-cytochemical study)].
The activity and ultrastructural localization of lactate dehydrogenase (LDH) in the sensomotor cortex of the brain was examined in health and after intake of a single 15-mg/kg dose of aminazin. On ultrastructural examination the enzyme was detected in some mitochondria of the neuron body, its axons synapses. Mitochondria containing the product of the enzymatic reaction were discovered also in the cells of the oligodendroglia, endothelium, and pericytes. After the aminazin intake the LDH activity (as shown by photometric findings) increased both in visible neurons and neuropil, with simultaneous decrease of the number of neurons containing the reaction product. This correlated with electron microscopic and cytochemical findings. On these examinations hypochromic neurons containing a great number of active mitochondria and lipofuscin granules with positive and negative reactions were revealed. On the other hand, hyperchromic neurons containing no reaction product were also seen.
D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology*; D002746:Chlorpromazine / Q000494:pharmacology*; D004727:Endothelium / Q000201:enzymology; D006651:Histocytochemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008854:Microscopy, Electron; D008928:Mitochondria / Q000201:enzymology; D051381:Rats; D011919:Rats, Inbred Strains
D007770:L-Lactate Dehydrogenase; D002746:Chlorpromazine
Raspredelenie aktivnosti laktatdegidrogenazy v kore golovnogo mozga v norme i posle odnokratnogo vvedeniia aminazina (gistokhimicheskoe i élektronno-tsitokhimicheskoe issledovanie).