Characterization of gastric-mucosal membranes. Distribution of lipid- and protein-associated amino groups across pig gastric microsomes.
Two NH2-reactive probes (2,4,6-trinitrobenzesulphonic acid and 1-fluoro-2,4-dinitrobenzene) were used to study the vectorial orientation of the membrane-associated free NH2 groups across pig gastric microsomal vesicles. Unlike 1-fluoro-2,4-dinitrobenzene, 2,4,6-trinitrobenzenesulphonic acid is ordinarily an impermeant probe that becomes permeant in the presence of K+ and valinomycin. Although 2,4,6-trinitrobenzenesulphonic acid alone reacts with about 28% of the total microsomal phosphatidylethanolamine, 2,4,6-trinitrobenzenesulphonic acid in the presence of valinomycin plus K+ or 1-fluoro-2,4-dinitrobenzene alone reacted with 75% of the phosphatidyl- ethanolamine. Under similar conditions the free NH2 groups associated with the microsomal proteins also exhibited an asymmetric labeling pattern, the intra- and extravesicular orientation being 74 and 26% respectively.
D000818:Animals; D004139:Dinitrofluorobenzene; D005753:Gastric Mucosa / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008563:Membrane Lipids / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D013552:Swine; D014302:Trinitrobenzenesulfonic Acid; D014634:Valinomycin / Q000494:pharmacology
D008563:Membrane Lipids; D008565:Membrane Proteins; D010714:Phosphatidylethanolamines; D014634:Valinomycin; D014302:Trinitrobenzenesulfonic Acid; D004139:Dinitrofluorobenzene; D011188:Potassium