Absence of the iron-containing superoxide dismutase in mitochondria from mustard (Brassica campestris).
Mitochondria were isolated from mature leaves as well as etiolated seedlings of Brassica campestris (mustard), a eukaryote previously shown to possess the iron-containing isoenzyme of superoxide dismutase. On the basis of KCN- and H2O2-sensitivity, and on polyacrylamide-gel analysis, only the cuprozinc and mangano superoxide dismutases were found in mitochondria. The iron-containing enzyme was absent. The mangano enzyme was found in the mitochondrial matrix, whereas the cuprozinc enzyme appeared to be localized in the intermembrane space.
D003300:Copper; D004591:Electrophoresis, Polyacrylamide Gel; D007501:Iron; D007527:Isoenzymes / Q000032:analysis*; D008345:Manganese; D008928:Mitochondria / Q000201:enzymology*; D009149:Mustard Plant / Q000201:enzymology; D010944:Plants / Q000201:enzymology*; D010946:Plants, Medicinal; D013482:Superoxide Dismutase / Q000032:analysis*
D007527:Isoenzymes; D008345:Manganese; D003300:Copper; D007501:Iron; D013482:Superoxide Dismutase