Ribosomal core-particles as the target of ricin.
Core-particles and split-proteins were prepared by treatment with ethanol and NH4Cl of control and ricin-treated Artemia salina ribosomes. No modifications of the ricin-treated split-proteins was detected by polyacrylamide-gel electrophoresis. Moreover, the split-proteins from ricin-treated ribosomes complemented control core-particles in poly(U)-directed phenylalanine polymerization. Conversely, ricin-treated core-particles remained totally inactive when supplemented with control split-proteins.
D000818:Animals; D001156:Artemia / Q000378:metabolism; D066298:In Vitro Techniques; D010649:Phenylalanine / Q000378:metabolism; D011072:Poly U / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects*; D012276:Ricin / Q000494:pharmacology*
D012269:Ribosomal Proteins; D011072:Poly U; D010649:Phenylalanine; D012276:Ricin