Effect of exogenous iron on the viability of pathogenic Naegleria fowleri in serum.
When Naegleria fowleri (Lee) was incubated in newborn calf and human serum an amebicidal effect was observed. Heat inactivation of both sera resulted in the recovery of viable amebae after incubation in these sera. Exogenous iron added to non-heat inactivated calf serum improved viability slightly but was without effect when added to human serum not heat inactivated. Exogenous iron greatly enhanced growth and/or viability in heat inactivated calf serum. Viability of amebae also was considerably enhanced in human serum which was heat inactivated when pH was lowered in conjunction with iron supplements.
D000656:Amoeba / Q000187:drug effects*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D007501:Iron / Q000494:pharmacology*
D003470:Culture Media; D007501:Iron