title: Carbohydrate content of middle molecular weight substances (MMWS) in uraemic patients: preliminary results.
abstract: MMWS (between 500 and 10,000 daltons) have been isolated from peritoneal dialysis fluid. Materials were fractionated according to a procedure previously applied to urine in healthy controls and in patients with various glycoproteins. Successive use of ion-exchange, charcoal-Celite and paper chromatography leads to the isolation of 20 oligosaccharidic fractions whose MW range between 400 and 2000 daltons. Glucuronoconjugates were also identified by this technique. Preliminary studies show that this carbohydrate material is very similar to the one found in normal urine. The origin and the signification of these oligosaccharides will be discussed in regard with our present knowledges on the structure, catabolism, and function of glycoproteins.
mesh_terms: D002241:Carbohydrates / Q000032:analysis*; D002854:Chromatography, Paper / Q000379:methods; D006595:Hexosamines / Q000032:analysis; D006801:Humans; D009844:Oligosaccharides / Q000032:analysis; D010530:Peritoneal Dialysis; D012794:Sialic Acids / Q000032:analysis; D014118:Toxins, Biological / Q000302:isolation & purification*; D014511:Uremia / Q000378:metabolism*; D014574:Uronic Acids / Q000032:analysis
chemical_list: D002241:Carbohydrates; D006595:Hexosamines; D009844:Oligosaccharides; D012794:Sialic Acids; D014118:Toxins, Biological; D014574:Uronic Acids; C026865:uremia middle molecule toxins