title: Determination of the alpha,beta-adrenoceptor blocker YM-09538 in plasma by high-performance liquid chromatography with fluorescence detection.
abstract: A high-performance liquid chromatographic method for the determination of the alpha,beta-adrenoceptor blocker 5-(1-hydroxy-2-[2-(o-methoxyphenoxy)ethylamino]ethyl]-2-methyl-benzenesulphonamide hydrochloride (YM-09538) in plasma, using 5-di-n-butylaminonaphthalene-1-sulphonyl chloride as a reagent for fluorescence labelling is described. The detection limit is 20 ng/ml, which is sensitive enough to determine YM-09538 plasma levels after the oral administration of effective doses to dogs and human.
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D004983:Ethanolamines / Q000008:administration & dosage / Q000097:blood*; D006207:Half-Life; D006801:Humans; D000096926:Benzenesulfonamides
chemical_list: D004983:Ethanolamines; C032473:amosulalol; D000096926:Benzenesulfonamides