[Anticoagulating activity and lytic action of the heparin-antithrombin III and antithrombin III-heparin-thrombin complexes on non-stabilized fibrin].
The heparin-antithrombin III and antithrombin III-heparin-thrombin complexes were prepared by rapid mixing of the components at different weight ratios (1:1, 1:3, 1:4, 1:6, 1:7, 3:1 - heparin/antithrombin III), pH 7.0-7.2. The reaction mixture was incubated for 15 min at 37 degrees. The complexes were precipitated at pH 4.5-4.8. All the heparin-antithrombin III complexes possess high anticoagulating and lytic activities. The antithrombin III-heparin-thrombin complex prepared at the weight ratio of 1:1:6 has a high lytic activity. The antithrombin III-heparin-thrombin complex prepared at the weight ratio of 1:1:1 reveals a high anticoagulating activity and a strong lytic action with respect to the non-stabilized fibrin.
D000990:Antithrombin III / Q000378:metabolism*; D001777:Blood Coagulation; D004355:Drug Stability; D005337:Fibrin / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D046911:Macromolecular Substances; D013917:Thrombin / Q000378:metabolism*
D046911:Macromolecular Substances; D000990:Antithrombin III; D005337:Fibrin; D006493:Heparin; D013917:Thrombin
Kompleksy geparin-antitrombin III, antitrombin III-geparin-trombin, ikh antikoaguliantnaia aktivnost' i liticheskoe deĭstvie na nestabilizirovannyĭ fibrin.