An improved technique for perfusion of the guinea pig placenta in situ giving viable conditions demonstrated by placental transport of amino acids (L- and D-alanine).
An improved technique for perfusing the fetal guinea pig placenta in situ is described. The result shows linear pressure-flow relations in the rane of 15-50 mm Hg. assumed to be physiological. The preparation shows impermeability for trypan blue. The placental transfer is stereoselective for L-alanine compared with D-alanine. This transfer is ouabain-sensitive, and proceeds against a concentration gradient.
D000409:Alanine / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D005260:Female; D006168:Guinea Pigs; D010042:Ouabain / Q000494:pharmacology; D010477:Perfusion / Q000379:methods*; D010539:Permeability; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011312:Pressure; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D014343:Trypan Blue / Q000378:metabolism
D010042:Ouabain; D014343:Trypan Blue; D000409:Alanine