title: Control of passive permeability of Chinese hamster ovary cells by external and intracellular ATP.
abstract: External ATP causes passive permeability change in several transformed cells, but not in untransformed cells. We studied the effect of external ATP on the passive permeability of CHO-K1 cells, a transformed clone of Chinese hamster ovary cells. Treatment of the cells with external ATP alone did not produce a permeability change, and this was observed only when a mitochondrial inhibitor, such as rotenone or oligomycin, was present together with ATP. These inhibitors reduced the concentration of intracellular ATP and a permeability change by external ATP was observed when intracellular ATP was decreased more than 70%. This requirement for permeability change of CHO-K1 cells was quite unique, since passive permeability change of other transformed cells so far tested was induced by ATP alone. Treatment of CHO-K1 cells with cyclic AMP analogues increased their sensitivity to external ATP about 2-fold. The roles of external and intracellular ATP in controlling passive permeability are discussed.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D007700:Kinetics; D009596:Nitrophenols / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*; D012402:Rotenone / Q000494:pharmacology
chemical_list: D009596:Nitrophenols; D009943:Organophosphorus Compounds; D012402:Rotenone; C008644:nitrophenylphosphate; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase