[A simple method for the detection of staphylococcal enterotoxin type B in vanilla custard using the ELISA (author's transl)].
The ELISA for the detection of staphylococcal enterotoxin type B (SEB) was employed to demonstrate SEB in Dutch Vanilla custard. Due to the sensitivity of the ELISA the extraction procedure, which is necessary when the Ouchterlony test is used, can be abbreviated to a great extent. Two successive extractions at pH 7.4 and pH 4.5 followed by a concentration (1:20) was sufficient to detect 0.1 mcg SEB in 100 g custard.
D004768:Enterotoxins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005504:Food Analysis; D005511:Food Handling
D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal
Einfache Methode für den Nachweis von Staphylokokken-Enterotoxin-B in Vanillepudding mittels ELISA-Test.