Calcium-binding constants of trypsin and trypsinogen. A reassessment.
The Ca2+-binding constants for trypsin and trypsinogen have been reassessed by using enzyme that has been purified by affinity chromatography and measuring the distribution of 45Ca2+ between the protein and a cation exchanger. The pKCa2+ value of 4.5 for the high-affinity site on trypsin was 1 logarithmic unit greater than that previously reported.
D002118:Calcium / Q000378:metabolism*; D002846:Chromatography, Affinity; D011485:Protein Binding; D014357:Trypsin / Q000302:isolation & purification / Q000378:metabolism*; D014362:Trypsinogen / Q000302:isolation & purification / Q000378:metabolism*
D014362:Trypsinogen; D014357:Trypsin; D002118:Calcium