Cell-surface radioiodination with the sparingly soluble catalyst Iodogen. Difference between the dividing and non-dividing populations of rodent thymocytes.
The surface proteins of dividing and non-dividing subpopulations of rat and mouse thymocytes have been labelled by using a new method of radioiodination. Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and autoradiography of the labelled proteins shows distinct differences in labelling between the mouse and rat cells and also, in the case of the rat, between the dividing and non-dividing populations.
D000818:Animals; D001345:Autoradiography; D002384:Catalysis; D002455:Cell Division; D004591:Electrophoresis, Polyacrylamide Gel; D007093:Imidazoles; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism; D014508:Urea / Q000031:analogs & derivatives
D007093:Imidazoles; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; D014508:Urea