title: Isolation and characterization of beta-lipotropin from the pituitary gland of the ostrich, Struthio camelus.
abstract: Avian beta-lipotropin (beta-LPH) was purified from adenohypophyseal glands of the ostrich Struthio camelus by a procedure involving acid/acetone extraction, NaCl fractionation, CM-cellulose chromatography, Sephadex G-75 chromatography and paper electrophoresis (pH 6.7). The 90-amino acid peptide behaved as a single substance during polyacrylamide-gel electrophoresis, isoelectric focusing (pI of 6.0) and N-terminal analysis, the N-terminal amino acid being alanine. Ostrich beta-LPH exhibited lipolytic activity corresponding to an average minimal effective dose of 0.088 micrograms in rabbit adipose tissue.
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000596:Amino Acids; D000818:Animals; D001717:Birds / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D011817:Rabbits; D013045:Species Specificity; D008083:beta-Lipotropin / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D008083:beta-Lipotropin