High-performance liquid chromatographic methods for base and nucleoside analysis in extracellular fluids and in cells.
High-performance liquid chromatography based methods for the study of the metabolism of purine and pyrimidine bases and nucleosides have been developed. These methods, using 200--50 microliter samples of extracellular fluids and employing isocratic separations, can measure a wide range of compounds. Hypoxanthine, xanthine and uridine concentrations in plasma from normal men are relatively stable. Species differences have been detected: concentrations of cytidine are higher in rat and mouse serum than in man, since the concentrations of uridine are similar; purine/pyrimidine ratios may be different. Fetal calf serum used for tissue culture contains about a 40 times higher concentration of hypoxanthine than the less-effective calf serum. Use of the methods appears to be justified in the assessment of the metabolic damage due to severe hypoxia and/or ischaemia.
D000818:Animals; D001773:Blood Cells / Q000032:analysis*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004285:Dogs; D005110:Extracellular Space / Q000032:analysis*; D005260:Female; D006801:Humans; D008253:Macaca mulatta; D008297:Male; D051379:Mice; D009705:Nucleosides / Q000032:analysis*; D009711:Nucleotides / Q000032:analysis*; D011247:Pregnancy; D011687:Purines / Q000032:analysis; D011743:Pyrimidines / Q000032:analysis; D051381:Rats; D012756:Sheep
D009705:Nucleosides; D009711:Nucleotides; D011687:Purines; D011743:Pyrimidines