Radioreceptor assay for benzodiazepines in biological fluids using a new dry and stable receptor preparation.
A method for determination of benzodiazepines in human blood, plasma, saliva and urine has been developed. The method is based upon the competition between 3H-flunitrazepam and biologically active benzodiazepines in biological fluids for brain specific receptors, prepared in a stable, dry form and easy to handle. The pharmacological specificity for benzodiazepines of the dry stable receptor preparation is closely similar to that of fresh membrane-bound rat brain receptors. The method is specific for biologically active benzodiazepines; it is relatively rapid, sensitive and reproducible, and can be performed at room temperature.
D001569:Benzodiazepines / Q000032:analysis* / Q000097:blood / Q000652:urine; D001826:Body Fluids / Q000032:analysis*; D001923:Brain Chemistry; D004355:Drug Stability; D005445:Flunitrazepam; D006801:Humans; D011208:Powders; D011869:Radioligand Assay / Q000379:methods*; D012463:Saliva / Q000032:analysis
D011208:Powders; D001569:Benzodiazepines; D005445:Flunitrazepam