title: Transmissible plasmids from Campylobacter jejuni.
abstract: Tetracycline resistance in clinical isolates of Campylobacter jejuni was shown to be plasmid mediated. Intra- and interspecies transfers to C. fetus subsp. fetus were demonstrated. The frequency of transfer was increased by approximately 100-fold on a solid surface by using a plate- or filter-mating procedure, as compared with a liquid-mating method. Results of experiments in which cell-free filtrates were used to replace the donor strain in mating experiments tend to rule out bacteriophage-mediated transduction in the transfer of tetracycline resistance. The plasmid-transfer frequency was not affected when deoxyribonuclease was added to the agar used in the mating experiments, indicating that transformation was not involved. Four transmissible plasmids from different tetracycline-resistant strains of C. jejuni each had a molecular weight of 38 x 10(6). Transfer of these plasmids to Escherichia coli was not demonstrated.
mesh_terms: D002167:Campylobacter / Q000032:analysis / Q000187:drug effects / Q000235:genetics*; D002169:Campylobacter Infections / Q000382:microbiology; D002168:Campylobacter fetus / Q000032:analysis / Q000187:drug effects / Q000235:genetics*; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis; D004270:DNA, Circular / Q000032:analysis; D006801:Humans; D008970:Molecular Weight; D011815:R Factors
chemical_list: D004269:DNA, Bacterial; D004270:DNA, Circular