[Use of the flow cytofluorometric method for analysing the interaction of low-density lipoproteins with cells].
To analyse the interaction of low density lipoproteins (LDL) with cells, the method of flow cytofluorometry (FCF) and labelled by rodamine isothiocyanate LDL (R-LDL) was used for the first time. This method may be used to analyse the incorporation of R-LDL in cells both in suspension cultures and in cell cultures attached to the underlie. The FCF method makes it possible to evaluate the incorporation of R-LDL in single cells, therefore it is more reasonable to use it while working with small and heterogeneous cell populations. With suspension cultures, the FCF method does not require cell washing from unbound label which makes the procedure more simple. The perspectives of the use of the FCF method for the analysis of the interaction of cells with LDL are connected with a possibility of selection and isolation of cells from heterogeneous populations.
D001011:Aorta / Q000166:cytology*; D001792:Blood Platelets / Q000378:metabolism*; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000032:analysis* / Q000378:metabolism; D013535:Suspensions; D014471:Umbilical Veins / Q000166:cytology*
D008077:Lipoproteins, LDL; D013535:Suspensions
Primenenie metoda potochnoĭ tsitofliuorimerii dlia analiza vzaimodeĭstviia lipoproteidov nizkoĭ plotnosti s kletkami.