title: Electron microscopic mapping of wheat germ RNA polymerase II binding sites on cloned CaMV DNA.
abstract: The binding sites of wheat germ RNA polymerase II were mapped on the cloned CaMV genome by observation of enzyme-linear DNA complexes by electron microscopy. Twelve sites are observed. Three of them are relatively stable in the presence of heparin and are found at positions 8-9, 21-23, and 41-44 map units on the physical map of the genome. These positions correspond to AT-rich regions of the viral genome which contain potential promoter sites. These results are discussed with reference to current information on the structure and expression of the CaMV genome.
mesh_terms: D001665:Binding Sites; D002874:Chromosome Mapping; D004274:DNA, Recombinant / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D005814:Genes, Viral; D006493:Heparin / Q000494:pharmacology; D008854:Microscopy, Electron; D009029:Mosaic Viruses / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D012319:RNA Polymerase II / Q000235:genetics*; D014908:Triticum / Q000235:genetics
chemical_list: D004274:DNA, Recombinant; D006493:Heparin; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases