[Inhibiting effect of adrenaline on the acetylcholinesterase activity of skeletal muscle sarcolemma].
It has been found that epinephrine reversibly inhibits in vitro the acetylcholinesterase activity of intact sarcolemma and partially purified preparation of sarcolemmal acetylcholinesterase. The kinetics analysis has demonstrated a non-competitive type of inhibition with the apparent Ki values of about 4 . 10(-4) M. An increase in the ionic strength intensities the epinephrine-induced inhibition, the effect of CaCl2 being essentially more noticeable than that of NaCl. The number of SH-groups of the sarcolemma available for DTNB decreases in the presence of epinephrine and increases in the presence of CaCl2 or NaCl. The sulfhydryl reagents (e. g. DTNB, NEM) have no effect n the acetylcholinesterase activity of sarcolemma. It is assumed that the inhibiting effect of epinephrine may be due to the conformational changes in the acetylcholinesterase molecules.
D000818:Animals; D002800:Cholinesterase Inhibitors; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology*; D005033:Ethylmaleimide / Q000494:pharmacology; D007700:Kinetics; D009132:Muscles / Q000201:enzymology*; D009994:Osmolar Concentration; D011817:Rabbits; D012508:Sarcolemma / Q000201:enzymology*
D002800:Cholinesterase Inhibitors; D004228:Dithionitrobenzoic Acid; D005033:Ethylmaleimide; D004837:Epinephrine
Ingibirovanie adrenalinom atsetilkholinésteraznoĭ aktivnosti sarkolemmy skeletnykh myshts.