Aryl ether O-dealkylase activity in the skin of untreated mice in vitro.
1. O-Dealkylation of p-nitroanisole and p-nitrophenetole in 10 000 g supernatant preparations of mouse skin is detectable and quantifiable. The reaction is NADPH-dependent and is mediated by cytochrome P-450. 2. The rate of p-nitroanisole O-demethylation in mouse skin preparations is 2% of that in mouse liver preparations on a mg protein basis, but only 0.23% on a g of tissue basis. 3. For p-nitrophenetole O-deethylation, the cutaneous Vmax is 3.8% of the hepatic Vmax on a mg protein basis. The Km values for the reaction in these two tissues are in a 3:1 ratio, suggesting that there are no marked qualitative differences between cutaneous and hepatic cytochrome P-450.
D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003640:Dealkylation; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D009578:Nitrobenzenes / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D010090:Oxidoreductases, O-Demethylating / Q000378:metabolism; D012867:Skin / Q000201:enzymology*
D009578:Nitrobenzenes; D002248:Carbon Monoxide; D003577:Cytochrome P-450 Enzyme System; C026048:4-nitroanisole O-demethylase; C031958:4-nitrophenetole-O-dealkylase; D010088:Oxidoreductases; D010090:Oxidoreductases, O-Demethylating; C013154:4-nitrophenetol