Chemical synthesis of 27-desamidosecretin gene by the polymer support method.
The polymer support method for the oligonucleotide synthesis has been developed and used for the chemical synthesis of a 27-desamidosecretin gene. The gene carrying Pst I recognition sites at both ends was built from 16 fragments and each of them was synthesized by a stepwise addition of protected nucleotides on a polymer support. The coupling yield of each step was almost 90% and after partial deblocking, oligonucleotides carrying trityl groups were purified by chromatography on a reverse phase column. The recombinant DNA containing this synthetic gene was expressed in bacterial cells.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis*; D005796:Genes; D007202:Indicators and Reagents; D007413:Intestinal Mucosa / Q000378:metabolism; D012633:Secretin / Q000031:analogs & derivatives* / Q000235:genetics; D013552:Swine
D007202:Indicators and Reagents; C032880:secretin, 27-deamido-; D012633:Secretin; D004247:DNA