Detection of circulating metallothionein in rats injected with zinc or cadmium.
Circulating metallothionein was measured by radioimmunoassay over a 13-day period in male Sprague-Dawley rats that received a sequence of three intraperitoneal injections (at 3-day intervals) of either 5 milligrams of zinc or 0.8 milligrams of cadmium per kilogram of body weight. These amounts of zinc and cadmium produced metallothionein concentrations in the range of 2 to 5 nanograms per milliliter of serum (zinc) and 2 to 15 nanograms per milliliter of serum (cadmium). In control rats given saline injections over the same period the metallothionein concentration ranged from 1 to 3 nanograms per milliliter of serum.
D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008297:Male; D008667:Metalloproteins / Q000097:blood*; D008668:Metallothionein / Q000097:blood* / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats; D011919:Rats, Inbred Strains; D015032:Zinc / Q000494:pharmacology*
D008667:Metalloproteins; D002104:Cadmium; D008668:Metallothionein; D015032:Zinc