Bile acid induced interconversion of 3-hydroxy-3-methylglutaryl Coenzyme A reductase in cultured intestine.
The effect of bile acids and bile acid/cholesterol micelles on 3-hydroxy-3-methylglutaryl coenzyme A reductase, the key enzyme of cholesterol synthesis, was investigated in cultured intestine. Glycocholic and glycodeoxycholic acid both suppressed total (fully activated) reductase activity after 3 h culture. The portion of expressed reductase, determined in the presence of NaF, was unaffected at 3 h, but decreased after 24 h of bile acid treatment. In contrast, total enzyme activity was stimulated up to 2.5-fold at 24 h; this bile acid effect was blocked by additional cholesterol. These results suggest that bile acids modulate both total reductase activity and the activation state of the enzyme in cultured intestine.
D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology*; D002784:Cholesterol / Q000096:biosynthesis; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D007082:Ileum / Q000201:enzymology*; D007413:Intestinal Mucosa / Q000201:enzymology; D008297:Male; D008823:Micelles; D009924:Organ Culture Techniques; D011817:Rabbits; D013997:Time Factors
D001647:Bile Acids and Salts; D008823:Micelles; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases