Measurement of sulindac and its metabolites in human plasma and urine by high-performance liquid chromatography.
A sensitive high-performance liquid chromatography assay for simultaneous measurement of sulindac and its major metabolites was developed. The extraction methods provided greater than 89% recovery of sulindac and its sulfone and sulfide metabolites from both plasma and urine. Complete resolution and accurate detection of the three compounds was achieved with a reversed-phase column, UV detection at 254 nm and a methanol-acetate buffer mobile phase. Levels of sulindac and its metabolites were determined in plasma and urine from four volunteers after oral administration of 200 mg Clinoril. Glucuronide conjugates in urine were measured after alkaline hydrolysis.
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007192:Indenes / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D013467:Sulindac / Q000008:administration & dosage / Q000097:blood* / Q000652:urine
D007192:Indenes; D013467:Sulindac