Effect of exogenous phospholipids on protein synthesis in confluent heart cell cultures.
Preincubation for 23 hr of confluent chick embryo heart cell cultures with increasing doses of a phospholipidic complex produces a progressive increase of [14C] leucine uptake and of its incorporation into proteins when compared to control cultures maintained with 0.5% foetal calf serum. Conversely, a longer time of exposure (47 and 71 hr) to the same doses of phospholipids exhibits a negative effect on both parameters. The changes in protein specific activity appear to be due to a real different capability of the cells in utilizing the amino acid taken up rather than to the changes in is endogenous availability.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D006321:Heart / Q000187:drug effects; D007930:Leucine / Q000378:metabolism; D009124:Muscle Proteins / Q000096:biosynthesis*; D009206:Myocardium / Q000378:metabolism*; D010743:Phospholipids / Q000494:pharmacology*
D009124:Muscle Proteins; D010743:Phospholipids; D007930:Leucine