Glutathione-S-transferase of human red blood cells; assay, values in normal subjects and in two pathological circumstances: hyperbilirubinemia and impaired renal function.
An assay for human erythrocyte glutathione-S-transferase is described. The procedure is both sensitive and reproducible; sampling and storage conditions are also investigated. Reference values are given for normal neonates, children and adults. The normal red cell glutathione-S-transferase activity decreases significantly during the first weeks of life and remains constant afterwards. A slight but significant increase is observed over 75 years. Sex difference has no influence on enzyme activity. A significant increase (up to fourfold) in red cell glutathione-S-transferase activity is noted in newborns with hyperbilirubinemia exceeding 135 mumol/l. Red cell glutathione-S-transferase is also significantly increased in hemodialysed subjects suffering from renal dysfunction.
D000925:Anticoagulants / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology*; D005982:Glutathione Transferase / Q000097:blood*; D006801:Humans; D006932:Hyperbilirubinemia / Q000201:enzymology*; D007674:Kidney Diseases / Q000201:enzymology*; D006435:Renal Dialysis; D013379:Substrate Specificity
D000925:Anticoagulants; D005982:Glutathione Transferase