Radioimmunoassay for human aldolase A.
A radioimmunoassay was developed for the direct quantification of aldolase A in human serum. The method is a double antibody radioimmunoassay using radioiodinated aldolase A4 homopolymer as ligand, chicken antibodies to aldolase A, and rabbit antibodies to chicken IgG. The lowest measurable amount by this method was 2 ng (0.01 U). The radioimmunoassay was shown to be specific for the aldolase A subunit, with no cross-reactivity with human aldolase B subunits or homopolymeric human aldolase C (C4). The immunoreactive aldolase A in the sera of 41 normal healthy subjects ranged from 130 to 210 ng/ml (0.81-1.31 U/1), with a mean of 171 /+- 39 ng/ml.
D003429:Cross Reactions; D005634:Fructose-Bisphosphate Aldolase / Q000097:blood*; D006801:Humans; D007106:Immune Sera; D007527:Isoenzymes / Q000097:blood*; D009132:Muscles / Q000201:enzymology; D011863:Radioimmunoassay / Q000379:methods
D007106:Immune Sera; D007527:Isoenzymes; D005634:Fructose-Bisphosphate Aldolase