[Inhibition of luciferase from fireflies Luciola mingrelica by inorganic salts].
The effects of inorganic salts (sodium and potassium acetate, magnesium and sodium sulfate, sodium chloride, potassium nitrate, sodium phosphate, sodium and magnesium perchlorate) on the bioluminescence reaction catalyzed by luciferase from the fireflies Luciola mingrelica have been studied. The salts tested inhibit the enzymatic oxidation of luciferin. The efficiency of the inhibition increases in the following order: CH3COO-approximately SO4(2-)approximately Cl-less than NO3-approximately HPO4(-2) less than ClO4-. The inhibition constants (Ki) at saturating concentrations of both substrates-luciferin (LH2) and ATP, the binding constants (Ks) of luciferase to the substrates and the inhibition constants for luciferin (Ki(LH2)) and for ATP (Ki(ATP)) have been determined. The inhibition by luciferin in non-competitive, that by ATP is mixed. A possible mechanism of inhibition is discussed.
D000818:Animals; D001517:Coleoptera / Q000201:enzymology*; D007700:Kinetics; D008156:Luciferases / Q000037:antagonists & inhibitors*; D012492:Salts / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D012492:Salts; D008156:Luciferases
Ingibirovanie liutsiferazy svetliakov Luciola mingrelica neorganicheskimi soliami.