Methods for tissue extraction of sodium diphenylhydantoinate.
Procedures (I and II) are described here for tissue extraction of sodium diphenylhydantoin. Both methods imply: a) cell lysis with distilled water and sodium hydroxide respectively, and b) protein precipitation. Procedure I utilizing trichloroacetic acid to a 20 per cent dilution in tissue homogenates and II requiring two protein precipitation stages, one with hot acetone and the second with concentrated hydrochloric acid. Sediment and acidified aqueous phases were extracted several times with chloroform. An experiment implied in vitro application of the radioactive compound to previously weighed portions of the brain, cerebellum, liver and kidney of gerbo mongol. Another experiment consisted in applying the same procedures for tissue drug extraction after injection of the marked compound. In both experiments recoveries ranged from 95 to 98 per cent with variation coefficients under 8 per cent and p less than 0.01. Therefore we recommend both procedures although the second yields more transparent end extracts.
D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods; D005849:Gerbillinae; D006801:Humans; D007202:Indicators and Reagents; D010672:Phenytoin / Q000032:analysis*; D012995:Solubility
D007202:Indicators and Reagents; D010672:Phenytoin