Purification of adenosine deaminase from human red blood cells.
Adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) has been purified in high yield from human erythrocytes using a simple procedure based on two chromatographic steps. In the first step enzyme is bound to a diaminohexane-Sepharose column and eluted with salt. The second strap is column chromatography on Separose-bound purine riboside prepared by cross-linking the riboside to the matrix with 1,4-butanediol-diglycidyl ether. The overall purification factor was approximately 750,000 and the purity was at least 96%. The procedure is suitable for small sample sizes and for purification of the variant forms of the enzyme obtained from a single individual.
D000241:Adenosine / Q000378:metabolism; D000243:Adenosine Deaminase / Q000097:blood / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D008722:Methods; D009700:Nucleoside Deaminases / Q000302:isolation & purification*; D013056:Spectrophotometry, Ultraviolet
D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D000241:Adenosine