[Determination of AMP-aminohydrolase activity by a chromatographic method].
A method is developed for determining the AMP-aminohydrolase activity by means of chromatography on the home KY-2 ion-exchange resin. It differs from the method of differential spectrophotometry by the fact that its application enables the content of IMP formed due to the enzymic reaction to be determined by its absolute quantity. This method yields satisfactory results independently of the substrate concentration. However, it cannot be applied for determination of the AMP-aminohydrolase activity in the presence of ATP.
D000659:AMP Deaminase / Q000378:metabolism*; D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D009132:Muscles / Q000201:enzymology*; D009709:Nucleotide Deaminases / Q000378:metabolism*; D011817:Rabbits; D013053:Spectrophotometry / Q000379:methods
D009709:Nucleotide Deaminases; D000659:AMP Deaminase
Opredelenie aktivnosti AMP-aminogidrolazy khromatograficheskim sposobom.