Separation of acidic drugs in the microgram/ml range in untreated blood plasma by direct injection on liquid chromatographic columns.
Conditions are described that enable the injection of untreated, non-diluted blood plasma on liquid chromatographic columns for the determination of two acidic drugs. The mobile phase is chosen so that the plasma proteins are kept in solution. The influence of the number of plasma injections on the column back-pressure and retention time have been studied. Quantitations could be made without sample losses.
D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D009288:Naproxen / Q000097:blood*; D012459:Salicylates / Q000097:blood*; D020156:Salicylic Acid
D012459:Salicylates; D009288:Naproxen; D020156:Salicylic Acid