[Determination of lactic dehydrogenase isoenzymes in precancerous and cancerous gastric mucosa].
We have studied modifications of LDH isoenzymes pattern in normal human gastric mucosa as well as in adenocarcinoma and precancerous lesions of the stomac (gastritis and ulcer); samples from the injured and the surrounding non-injured area were examined, drawing up the isoenzymes, using Tris-buffer pH 7,4 at 4 degrees C and performing the determination within 1 h - because of the high chronolability of the fractions LDH and LDH by cellogol electrophoresis separation. We have always noticed - especially in samples from adenocarcinoma- a shifting toward the M chains, with a clear increase of the fractions LDH4 and LDH5; this has been noticed even in the surrounding non-injured area.
D000230:Adenocarcinoma / Q000201:enzymology; D005753:Gastric Mucosa / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D011230:Precancerous Conditions / Q000201:enzymology*; D013274:Stomach Neoplasms / Q000201:enzymology*
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase
Determinazio degli isoenzimi della latticodeidrogenasi nella mucosa gastrica in corso di lesioni cancerose e precancerose.