title: Effect of pentoxifylline on single red cell deformability.
abstract: In the present investigation the effect of pentoxifylline on single red cell deformability was determined. The deformability of human red blood cells were measured in a Singlepore Erythrocytes Rigidometer (SER). The new method determines optoelectronically the passage of each individual cell through a singlepore membrane (6 micrometers diameter and 50 micrometers length) under a driving pressure gradient (delta p = 100 Pa, tau = 3 Pa). Venous human blood samples were taken, prepared and divided into three samples. Sample A served as a physiological control, while to sample B and C CaCl2-solution was added. After Ca2+ stress sample C was incubated with 0.36 mmol/l pentoxifylline. The medium passage time (MPT) for sample A was 43.3 +/- 6.5 ms and for sample B 87.5 +/- 14.7 ms. The addition of pentoxifylline to the Ca2+-treated red cell suspension (sample C) reduced the MPT to 51.6 +/- 11.3 ms.
mesh_terms: D000328:Adult; D002118:Calcium / Q000097:blood; D004910:Erythrocyte Membrane / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects*; D006801:Humans; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D010431:Pentoxifylline / Q000494:pharmacology*; D013805:Theobromine / Q000031:analogs & derivatives*
chemical_list: D013805:Theobromine; D010431:Pentoxifylline; D002118:Calcium