title: The effect of complement in adherent immune complexes on Fc and C3 receptor expression in human monocytes.
abstract: The effect of complement in surface-bound immune complexes on the expression of Fc and C3 receptors in membranes of adherent human monocytes was examined. Monocytes were isolated from mononuclear leucocyte preparations by adherence to substrates containing fibrin, fibrin with immune complexes (containing rabbit IgG antibodies), or fibrin with immune complexes and mouse complement. Fc or C3 receptors on the top or exposed surface of the monocytes were detected by rosette formation with sheep erythrocytes coated with IgG (EA) or IgM and complement (EAC). Monocytes adherent to surface-bound immune complexes exhibited an absence of EA rosette-forming ability without any change in EAC rosettes. This specific loss of Fc receptor function was induced more easily in freshly-isolated monocytes than in cells maintained in suspension culture for up to 7 days. The presence of complement in the immune complex substrates did not reverse the decrease in Fc receptors seen with freshly-isolated or cultured monocytes. Monocytes adherent to immune complexes and complement exhibited a decrease in C3 receptor function. This decrease was more readily induced in cells cultured for three days in the presence of serum than in freshly-isolated monocytes. Experiments performed with EAC or immune complex substrates relatively enriched in C3b or C3bi indicated that C3b in the substrate induced a decrease in monocyte C3b receptors and C3bi led to a decrease in C3bi receptors. No evidence was found for C3d receptors on the human monocytes although these receptors on a subpopulation of human lymphocytes appeared to be altered in a similar fashion.
mesh_terms: D000936:Antigen-Antibody Complex; D002448:Cell Adhesion; D002478:Cells, Cultured; D003176:Complement C3 / Q000276:immunology*; D006801:Humans; D009000:Monocytes / Q000276:immunology*; D011951:Receptors, Complement / Q000276:immunology*; D011961:Receptors, Fc / Q000276:immunology*; D012397:Rosette Formation
chemical_list: D000936:Antigen-Antibody Complex; D003176:Complement C3; D011951:Receptors, Complement; D011961:Receptors, Fc