[Affinity between CrIII and purified DNA, studied by competition with an intercalating agent: ethidium bromide].
The affinity between CrIII and purified calf- thymus DNA was studied at neutral pH by competition with ethidium bromide. Competition results indicated an affinity between CrIII and DNA of the order of 10(5) 1/mole. These results are in good agreement with previous results CrIII - DNA affinity was studied by the independent method of equilibrium dialysis and chromium dosage by atomic spectrometry.
D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002857:Chromium / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004996:Ethidium / Q000378:metabolism*; D007364:Intercalating Agents / Q000378:metabolism*; D013054:Spectrophotometry, Atomic; D013950:Thymus Gland / Q000032:analysis
D007364:Intercalating Agents; D002857:Chromium; D004247:DNA; D004996:Ethidium
Affinita fra CrIII e DnA purificato, studiata mediante 1'antagonismo con un intercalante: il bromuro di etidio.