Direct spectrophotometric determination of serum and urinary oxalate with oxalate oxidase.
A new enzymatic method for direct photometric determination of oxalate in serum and urine is described, using oxalate oxidase. The resulting H2O2 is measured with a coupled enzyme system of catalase and aldehyde dehydrogenase. Percentage recovery of added oxalate was 99 +/- 4 in serum, and 98 +/- 4 in urine (n - 10). Oxalate serum levels varied from 16.9 to 44.8 mumol/l. Oxalate values can be determined within 20 minutes, without time consuming pretreatment of samples. The detection limit is 5 mumol/l.
D000444:Aldehyde Dehydrogenase; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D008832:Microchemistry / Q000379:methods; D010070:Oxalates / Q000032:analysis* / Q000378:metabolism; D019815:Oxalic Acid; D010088:Oxidoreductases / Q000378:metabolism*; D013053:Spectrophotometry / Q000379:methods
D010070:Oxalates; D019815:Oxalic Acid; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D002374:Catalase; D000445:Aldehyde Oxidoreductases; D000444:Aldehyde Dehydrogenase; C020598:oxalate oxidase