Isolation of a 5S RNA-protein L5 complex from 60S subunits of rat liver ribosomes by cesium sulfate density-gradient equilibrium centrifugation.
Upon CS2SO4 density-gradient equilibrium centrifugation, 60S subunits of rat liver ribosomes were dissociated to form three bands at the densities of 1.55, 1.40, and 1.30 g/ml. The bands at 1.55 and 1.30 g/ml were shown to contain exclusively RNA or proteins, respectively, whereas the band at 1.40 g/ml contained both RNA and a protein. The RNA in the 1.40 g/ml band was identified as 5S RNA and the protein in this band was protein L5. An empirical calculation suggested that the 1.40 g/ml band contained an equimolar 5S RNA-protein L5 ribonucleoprotein complex. This finding may indicate that protein L5 is located in very close proximity to 5S RNA in large ribosomal subunits.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002586:Cesium; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis*; D008297:Male; D009994:Osmolar Concentration; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000032:analysis*; D012995:Solubility
D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D002586:Cesium; C025407:cesium sulfate