Fluorimetric studies of the binding of Momordica charantia (bitter gourd) lectin with ligands.
The association constants for the binding of a series of ligands with a galactose-specific lectin from Momordica charantia (bitter gourd) has been determined through the ligand-induced quenching of protein fluorescence. Analysis of the iodide quenching suggested that there is a slight increase in the accessibility of tryptophan residues of the lectin on binding lactose.
D002241:Carbohydrates; D007785:Lactose; D037102:Lectins; D008024:Ligands; D037121:Plant Lectins; D012639:Seeds / Q000032:analysis*; D013050:Spectrometry, Fluorescence
D002241:Carbohydrates; D037102:Lectins; D008024:Ligands; D037121:Plant Lectins; D007785:Lactose