The effect of ampicillin on the response of human lymphocytes to PHA.
The effect of ampicillin on the sensitivity of human lymphocytes to PHA was examined by measuring the following parameters: temperature-stable E rosette formation: leukocyte migration inhibition: DNA synthesis. It was found, that ampicillin present in the culture medium at a concentration of 50 microgram/ml does not change E rosette formation, intensifies the inhibition of leukocyte migration and modulates the proliferative response to PHA. The magnitude and direction of this last effect depended upon the preparation of PHA used and on its dose. Ampicillin enhanced DNA synthesis in cultures stimulated by submitogenic, as well as supraoptimal doses of PHA-Wellcome and suppressed the DNA synthesis in cultures containing optimal mitogenic doses of PHA-Difco.
D000667:Ampicillin / Q000494:pharmacology*; D000818:Animals; D002464:Cell Migration Inhibition; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D012397:Rosette Formation; D012756:Sheep
D010835:Phytohemagglutinins; D000667:Ampicillin; D004247:DNA