The use of antiserum with specific reactivity toward fat-cell surface antigen(s) to follow the progression of 3T3-L1 preadipocyte differentiation in vitro.
Using an indirect, labelled-second-antibody cellular immunoassay technique, an adipocyte-specific antiserum has been investigated. Components of the antiserum were shown to bind to differentiated 3T3-L1 cells; the cellular capacity for binding increased progressively during the induced differentiation of these cells in vitro.
D000273:Adipose Tissue / Q000166:cytology* / Q000276:immunology; D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002454:Cell Differentiation; D002460:Cell Line; D007106:Immune Sera; D007118:Immunoassay; D008297:Male; D051379:Mice
D000954:Antigens, Surface; D007106:Immune Sera