[Changes in Bacillus pumilus antibiotic activity and sensitivity with integration and disintegration of plasmid pPL 7065].
The role of plasmid pPL 7065 in the control of the antibiotic production and sensitivity in Bac. pumilus was studied with the use a plasmid-free strain and strains containing the plasmid in the cells in a free or chromosome-integrated state. It was found that all the strains had antibiotic activity only with respect to a limited number of gram-positive bacteria. Still, the antibiotics produced by them differed in the antibacterial spectrum. The antibiotic produced by the plasmid-free strain had a broader spectrum. It was shown that the bacteria with the plasmid in th free state were sensitive to the antibiotics produced by the plasmid-free strain for the strain or the strain with the chromosome-integrated plasmid. It was suggested that plasmid pPL 7065 played the role of a modifier of the antibiotic activity and sensitivity in Bac. pumilus and carried no genetic information for the control of the antibiotic synthesis. Their formation is determined by the chromosome genes.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000494:pharmacology; D001407:Bacillus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010452:Peptide Biosynthesis; D010455:Peptides / Q000494:pharmacology; D010957:Plasmids
D000900:Anti-Bacterial Agents; D010455:Peptides
Izmenenie antibioticheskoĭ aktivnosti i chuvstvitel'nosti u bacillus pumilus pri integratsii i dezintegratsii plazmidy pPL 7065.