Effect of diphenylhydantoin, granatane-3-spiro-5'-hydantoin and leptazol on mouse brain glutamate dehydrogenase, urea and ammonia levels in brain.
The effect of diphenylhydantoin (DPH), granatane-3-spiro-5'-hydantoin and leptazol has been checked on both GDH activity and ammonia and urea levels in brain. Concentrations of 90 mg/kg of leptazol decreased, significantly, the ammonium and urea levels in brain with respect to normal control. DPH and G-3SH had no effect on these metabolites against normal control. The association of DPH and leptazol decreased both urea and ammonium levels in brain but the association of G-3SH and leptazol decreased, significantly, urea levels but not ammonium's. DPH, leptazol at 90 mg/kg concentration and the association of DPH or G-3SH with leptazol had no significant effect on GDH activity while G-3SH increased the enzyme activity when it was measured in the direction of glutamate degradation. DPH, G-3SH and both concentrations of leptazol decreased the GDH activity when it was checked in the direction of glutamate synthesis. The association of DPH and leptazol did not produce any effects, while the association of G-3SH-leptazol increased the enzyme activity. DPH and G-3SH and leptazol at 110 mg/kg concentration decreased the relation of GDH activity between the biosynthetic and degradative sense. The association of G-3SH-leptazol increased this relation while leptazol and its association with DPH did not affect it.
D000641:Ammonia / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004347:Drug Interactions; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006827:Hydantoins / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D010433:Pentylenetetrazole / Q000494:pharmacology*; D010672:Phenytoin / Q000494:pharmacology*; D014508:Urea / Q000378:metabolism
D006827:Hydantoins; D010672:Phenytoin; D000641:Ammonia; C033133:granatane-3-spiro-5'-hydantoin; D014508:Urea; D005969:Glutamate Dehydrogenase; D010433:Pentylenetetrazole