Chromatographic determination of thiodiglycolic acid - a metabolite of vinyl chloride.
The determination of thiodiglycolic acid (TDGA) In urine by gas chromatography (GC) is described. The analytical procedure includes addition of internal standard (o-phthalic acid), ethyl acetate extraction, evaporation of the solvent and silylation of TDGA with N-trimethylsilyl-diethylamine in pyridine (1:1). The results are calculated from the ratio of TDGA and internal standard peak heights. The detection limit of the method is 10 mg/l and the coefficient of various is +/- 5%.
D002849:Chromatography, Gas / Q000379:methods; D006801:Humans; D013864:Thioglycolates / Q000652:urine*; D014752:Vinyl Chloride / Q000378:metabolism*; D014753:Vinyl Compounds / Q000378:metabolism*
D013864:Thioglycolates; D014753:Vinyl Compounds; C010518:thiodiacetic acid; D014752:Vinyl Chloride