A comparative study of analytical methods to determine postmortem changes in carbon monoxide concentration.
Twenty-one autopsy blood samples were analyzed using spectrophotometric and gas chromatographic procedures after storage for 30 and 150 days. When carboxyhemoglobin was measured spectrophotometrically at the absorbance ratio of 540 nm/555 nm, the observed average percent losses were 8 +/- 9% and 35 +/- 27% after 30 and 150 days of storage, respectively. When measured at the absorbance ratio of 540 nm/579 nm, the average percent losses of carboxyhemoglobin were 7 +/- 8% and 34 +/- 25% after 30 and 150 days, respectively. Wavelength shifts and distorted spectral scans were observed at 150 days. When carbon monoxide was determined by gas chromatographic methods based on combining capacity, the average percent loss was 15 +/- 24% and 37 +/- 36% after 30 days and 150 days, respectively. The average percent loss of calculated CO based on hemoglobin concentration after 30 days was 31 +/- 14% and at 150 days, 40 +/- 24%. The average percent loss of calculated CO based on iron content was 23 +/- 13% and 37 +/- 23% after 30 and 150 days, respectively.
D002248:Carbon Monoxide / Q000097:blood*; D002263:Carboxyhemoglobin / Q000032:analysis; D002849:Chromatography, Gas; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007501:Iron / Q000097:blood; D011180:Postmortem Changes; D013053:Spectrophotometry; D013997:Time Factors
D006454:Hemoglobins; D002248:Carbon Monoxide; D002263:Carboxyhemoglobin; D007501:Iron