Immunosuppressive properties and circulating life of Achromobacter glutaminase-asparaginase covalently attached to polyethylene glycol in man.
The immunosuppressive effects and circulating life of Achromobacter glutaminase-asparaginase (GA) covalently attached to polyethylene glycol (PEG) were examined in human subjects following a single iv dose of 1000 IU/m2. Plasma half-life of PEG-GA was 72 hours. Skin test reactivity to recall antigens (mumps and tuberculin) was lost in all four patients tested. In vitro phytohemagglutinin-induced blastogenesis, "natural killing," and phytohemagglutinin-induced cell cytotoxicity was diminished as long as enzyme levels were detectable. In vivo and in vitro activities returned to normal following total plasma clearance of enzyme.
D000368:Aged; D000421:Alcaligenes / Q000201:enzymology* / Q000276:immunology; D001215:Asparaginase / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D002283:Carcinoma, Bronchogenic / Q000188:drug therapy* / Q000378:metabolism; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D005972:Glutaminase / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology*; D006207:Half-Life; D006801:Humans; D007958:Leukocyte Count; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011092:Polyethylene Glycols / Q000008:administration & dosage*
D011092:Polyethylene Glycols; D001215:Asparaginase; D005972:Glutaminase