[Comparison of analytical methods for the estimation of glycosylated haemoglobins (author's transl)].
The glycosylated haemoglobins of 26 different blood samples were analysed by six different methods; the regression lines and the correlation coefficients were calculated from the analytical data. The best separation and estimation of the fast haemoglobin fractions were achieved by high performance liquid chromatography (HPLC). Therefore HPLC is regarded as the reference method for the analysis of fast haemoglobin fractions. Microcolumn chromatography or the thiobarbituric acid method are recommended for routine analysis in clinical laboratories. Since the labile aldimine (Schiff's base) can be calculated from the analytical data or destroyed by appropriate methods, this glycosylated haemoglobin fraction does not interfere with the results.
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D013053:Spectrophotometry
D006442:Glycated Hemoglobin
Analyseverfahren für glykosidierte Hämoglobine. Ein Methodenvergleich.