Isolation of soluble immune complexes from human serum: combined use of polyethylene glycol precipitation, gel filtration, and affinity chromatography on protein A-Sepharose.
D000906:Antibodies / Q000032:analysis; D000936:Antigen-Antibody Complex / Q000302:isolation & purification*; D000941:Antigens / Q000032:analysis; D011232:Chemical Precipitation; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D003920:Diabetes Mellitus / Q000276:immunology; D006801:Humans; D007330:Insulin Antibodies / Q000032:analysis; D011092:Polyethylene Glycols; D012217:Rheumatoid Factor / Q000276:immunology; D012685:Sepharose; D013205:Staphylococcal Protein A
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000941:Antigens; D007330:Insulin Antibodies; D013205:Staphylococcal Protein A; D011092:Polyethylene Glycols; D012217:Rheumatoid Factor; D012685:Sepharose