title: Alkylation of DNA by organophosphorus pesticides.
abstract: Methods for the detection of in vivo alkylation of nucleic acids are discussed. Alkylation of mammalian DNA at N-7 of guanine provides the most sensitive and least equivocal evidence of methylation by an exogenous chemical or its metabolite. No 7-methylguanine was detected in the combined DNA from the lungs, livers, hearts, brains, kidneys, testes and spleens from 20 rats exposed to 0.064 micrograms l-1 of [Me-14C]-dichlorvos at a specific radioactivity of 113 Ci mol-1 for 12 hrs.
mesh_terms: D000477:Alkylating Agents; D000818:Animals; D004247:DNA / Q000378:metabolism*; D004006:Dichlorvos / Q000494:pharmacology; D006147:Guanine / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D008745:Methylation; D051381:Rats
chemical_list: D000477:Alkylating Agents; D007306:Insecticides; D006147:Guanine; D004006:Dichlorvos; D004247:DNA