A comparative ultrastructural study of the low virulent strain PV-C1/81 and RH strain cultured in A9, Vero, SIRC cell lines.
A human Toxoplasma strain, isolated in mice from bioptic lymphoglandular tissue, has been cultured in vitro in continuous cell lines. Its presence in some parastized cells, with parasitophorous vacuoles surrounded by a cyst-like wall and filled with a cystozoite-like clone, was observed by electron microscopy. These characteristic and constant features were confined in different cell line cultures and are suggested as possible markers of low virulent strains.
D000818:Animals; D001921:Brain / Q000469:parasitology; D002460:Cell Line; D002522:Chlorocebus aethiops; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D008247:Lysosomes / Q000648:ultrastructure; D008566:Membranes / Q000648:ultrastructure; D009940:Organoids / Q000469:parasitology*; D014122:Toxoplasma / Q000254:growth & development / Q000472:pathogenicity* / Q000648:ultrastructure; D014617:Vacuoles / Q000469:parasitology*