Rubella virosomes: preparation and ultrastructure.
Rubella virosomes were prepared from performed liposomes and detergent solubilized viral hemagglutinin. The liposomes were made from lecithin/dicetyl phosphate (3.5 : 1) films resuspended in NTE buffer and sonicated. Viral hemagglutinin was prepared from purified virus after solubilization with Triton X-100 and centrifugation through a sucrose gradient containing beta-D-octylglucoside. Electron microscopy of the rate zonal purified virosomes, showed virus-like structures of 40 - 80 nm. The virosomes retained the biological activity of the hemagglutinin and had a buoyant density of 1.2 g/cm3.
D002499:Centrifugation, Density Gradient; D006023:Glycoproteins / Q000032:analysis; D006389:Hemagglutinins, Viral / Q000032:analysis / Q000302:isolation & purification; D008081:Liposomes / Q000032:analysis; D008854:Microscopy, Electron; D012412:Rubella virus / Q000276:immunology*; D014764:Viral Proteins / Q000032:analysis
D006023:Glycoproteins; D006389:Hemagglutinins, Viral; D008081:Liposomes; D014764:Viral Proteins