Incorporation of bacteriophage DNA into the genome of cultured human lymphocytes.
After exposure of phytohemagglutinin-stimulated human lymphocytes to high doses of tritiated-thymidine labelled phi X-174 or T2 bacteriophage, label from the phage genome became incorporated into lymphocyte DNA. Exposure to bacteriophage DNA, whether biologically active, inactive, or fragmented, had a depressive effect on lymphocyte DNA replication. Incorporation of label from phage DNA into the lymphocyte DNA, however, was maximum for biologically active phage.
D002478:Cells, Cultured; D003090:Coliphages / Q000235:genetics*; D004247:DNA / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000502:physiology*; D014170:Transformation, Genetic
D004279:DNA, Viral; D004247:DNA