Effect of ascorbic acid and ferrous iron on p-aminohippurate accumulation by rat kidney cortical slices.
The effect of ascorbic acid and Fe2+ on p-aminohippurate accumulation by rat kidney slices was examined. Ascorbic acid and Fe2+ promoted lipid peroxidation of rat renal cortical slices was found to be dose related. Moreover, ascorbic acid (1.0 mM) and Fe2+ (0.4 mM) increased tissue water and decreased the accumulation of p-aminohippurate. The addition of N,N'-diphenyl-p-phenylenediamine (antioxidant) at the concentration of 1 x 10(-6) M recovered the accumulation of p-aminohippurate by about 43%. The apparent Km of p-aminohippurate uptake was increased by ascorbic acid and Fe2+ with no change in the apparent V. These data suggest that ascorbic acid and Fe2+ can cause a significant alteration in p-aminohippurate and water transport of renal cortical slices and that these effects may be, at least, partly due to the lipid peroxidative action of ascorbic acid and Fe2+.
D000618:Aminohippuric Acids / Q000378:metabolism*; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001834:Body Water / Q000378:metabolism; D005296:Ferrous Compounds / Q000494:pharmacology*; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology*; D007672:Kidney Cortex / Q000378:metabolism*; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D010655:Phenylenediamines / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D010130:p-Aminohippuric Acid / Q000378:metabolism*
D000618:Aminohippuric Acids; D005296:Ferrous Compounds; D008054:Lipid Peroxides; D010655:Phenylenediamines; C004845:N,N'-diphenyl-4-phenylenediamine; D007501:Iron; D001205:Ascorbic Acid; D010130:p-Aminohippuric Acid