Cytogenetics of murine thymic lymphomas induced by N-methyl-N-nitrosourea: difference in incidence of trisomy 15 in thymic lymphomas induced in neonatal and adult mice.
Chromosome complements of murine thymic lymphomas induced by an alkylating agent N-methyl-N-nitrosourea (MNUA) were analyzed microscopically and karyotypically using the Q-banding technique. The chemical carcinogen was injected intraperitoneally into either neonatal or 7-week-old CFW/D mice. In addition, thymic lymphomas induced in 7-week-old AKR mice and thymic lymphomas developed spontaneously in this strain were also examined. All six lymphomas induced in neonatal CFW/D had hyperdiploid cell lines that accounted for 90% of the cells analyzed. Chromosome analysis of lymphomas induced in adult DFW/D mice showed that only out of nine lymphomas had predominantly hyperdiploid cell lines. The remaining five lymphomas had diploid modal chromosome number although they also carried a variant line characterized by 41 chromosomes. All eight lymphomas induced in adult AKR mice and six out of seven spontaneous AKR lymphomas showed predominantly diploid modal line. The remaining spontaneous lymphoma had a hyperdiploid stem line of 41 chromosomes. Microscopic and karyotypic analysis further identified trisomy 15 as the regular chromosome abnormality in the hyperdiploid cells in lymphomas of each group, whereas cells with diploid chromosome number had no detectable chromosome abnormality. Additional trisomies were also found, but their appearance was restricted to individual tumors. Thus, the incidence of trisomy 15 in lymphomas induced by MNUA in adult CFW/D and AKR mice, as well as in the spontaneous AKR lymphomas, is significantly lower than that in lymphomas induced in neonatal mice by the same carcinogen.
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D007621:Karyotyping; D008223:Lymphoma / Q000139:chemically induced / Q000235:genetics*; D008770:Methylnitrosourea; D051379:Mice; D008815:Mice, Inbred Strains; D009374:Neoplasms, Experimental / Q000235:genetics; D013953:Thymus Neoplasms / Q000139:chemically induced / Q000235:genetics*; D014314:Trisomy
D008770:Methylnitrosourea