[Micromethod for the fluorometric determination of the polyamine and putrescine content in animal tissues by thin-layer chromatography on Silufol UV-254 plates].
A microquantitative, simple, rapid, highly sensitive procedure is described for estimation of polyamines and putrescine in animal tissues. Content of these substances was studied in several rapidly proliferating tissues. Statistically distinct increase in content of putrescine and polyamines was found in regenerating, malignant rat liver tissue and in hepatomas G-27 and G-46. The mean error of the assay was about 3%, its sensitivity was +/- 0.1 nmol per 1 mg of protein, reproducibility was close to 95-100%.
D000818:Animals; D001322:Autoanalysis / Q000295:instrumentation; D002855:Chromatography, Thin Layer / Q000295:instrumentation*; D003619:Dansyl Compounds / Q000032:analysis; D008099:Liver / Q000032:analysis; D008114:Liver Neoplasms, Experimental / Q000032:analysis; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011073:Polyamines / Q000032:analysis*; D011700:Putrescine / Q000032:analysis*; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods*
D003619:Dansyl Compounds; D011073:Polyamines; D011700:Putrescine
Mikrometod fliuorometricheskogo opredeleniia soderzhaniia poliaminov i putrestsina v tkaniakh zhivotnykh tonkosloĭnoĭ khromatografieĭ na plastinakh Silufol UV-254.