The use of horseradish peroxidase-lectin conjugates to monitor the purity of plasma-membrane preparations obtained from isolated cells.
The plasma membranes of viable murine EL4 tumour cells were labelled with horseradish peroxidase-conjugated wheat-germ agglutinin. After disruption of the labelled intact cells, plasma-membrane purification could be monitored by ultrastructural examination of the various fractions for positive reaction product on the membrane vesicles.
D000818:Animals; D002458:Cell Fractionation; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure*; D006735:Horseradish Peroxidase; D066298:In Vitro Techniques; D037102:Lectins; D046911:Macromolecular Substances; D008722:Methods; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D010544:Peroxidases
D037102:Lectins; D046911:Macromolecular Substances; D006735:Horseradish Peroxidase; D010544:Peroxidases