[Purification of the brain hormone of Nereis diversicolor and Perinereis cultrifera (Annelida:Polichaeta) by high-pressure liquid chromatography (HPLC)].
Five thousand brains inhibiting gametogenesis in Nereis diversicolor and Perinereis cultrifera were extracted with cold methanol, giving a micromolecular fraction containing the whole of the inhibitory activity of the brains. This micromolecular fraction, fractionated (i) on Sephadex G 25 fine and (ii) on Sephadex G 25 superfine, gave an inhibitory peak (P 5) in organ culture. Fraction P 5, purified by high-pressure liquid chromatography, showed an area with the same biological inhibitory activity as the blank brains.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007447:Invertebrate Hormones / Q000302:isolation & purification*; D008970:Molecular Weight; D011077:Polychaeta / Q000378:metabolism*
D007447:Invertebrate Hormones
Purification par chromatographie liquide de haute pression (HPLC) de l'hormone cérébrale chez Nereis diversicolor et Perinereis cultrifera (Annélides Polychètes).