Reevaluation of membrane binding of erythrocyte glutathione reductase (EC 1.6.4.2).
Data concerning membrane binding of glutathione reductase (GSSG-R) have been reexamined with respect to the following experimental conditions: 1. Haemolysis and membrane preparation; 2. influence of nonionic detergents (Triton X-100, Lubrol PX). Haemolysis and membrane preparations performed at physiological ionic strength, and reduced glutathione concentration do not give any evidence for binding of GSSG-R to the erythrocyte membrane. Under hypotonic conditions of ghost preparation about 2% of the total cellular GSSG-R activity are membrane bound, indicating weak enzyme association at low ionic strength only.
D000328:Adult; D003902:Detergents / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D005980:Glutathione Reductase / Q000097:blood*; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism; D009994:Osmolar Concentration
D003902:Detergents; D008565:Membrane Proteins; D005980:Glutathione Reductase