SEM images of block faces, cut by a miniature microtome within the SEM - a technical note.
A miniature microtome is described which functions within the specimen chamber of a typical SEM. SEM images are shown of successive faces of an Epon block of squid (Loligo pealei) fin nerve, cut with this instrument. The implications for 3-dimensional reconstruction are discussed.
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D049832:Decapodiformes; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D008867:Microtomy / Q000295:instrumentation / Q000379:methods*; D009420:Nervous System / Q000648:ultrastructure