Properties of cell subpopulations of starfish axial organ: in vitro effect of pokeweed mitogen and evidence of lymphokine-like substances.
The in vitro effects of pokeweed mitogen (PWM) on axial organ (AO) cells of the echinoderm starfish Asteria rubens have been studied. PWM stimulates 3H-thymidine incorporation by the whole population of AO cells (index of stimulation 4-5). On the basis of surface adherence to nylon wool the AO cell population can be fractionated into adherent and non-adherent subpopulations. PWM strongly stimulates the non-adherent cells (index 14) but not the adherent cells (index 1.2-1.8). Specific soluble mediators obtained from stimulated non-adherent cells were able to stimulate the whole AO cell population but not the fractionated adherent or non-adherent cells.
D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D008221:Lymphoid Tissue / Q000276:immunology*; D008222:Lymphokines / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D013215:Starfish / Q000276:immunology*
D008222:Lymphokines; D011043:Pokeweed Mitogens