title: A two-step procedure for the purificaton of hepatitis B surface antigen (HBsAg).
abstract: Hepatitis B surface antigen (HBsAg) was purified by a simple, rapid, two-step procedure comprising chromatography on dextran sulfate-Sepharose 4B, followed by preparative rate zonal centrifugation on a sucrose gradient. A 230-fold purification of HBsAg was achieved with a yield of about 80%.
mesh_terms: D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis
chemical_list: D006514:Hepatitis B Surface Antigens; D007075:Immunoglobulin M