Electrophysiological properties of tissue-cultured nerve cells from senescent mouse.
Nerve cells were isolated from dorsal root ganglia of senescent mouse (C57 black, male) by collagenase and were grown on collagen-coated plastic dishes. The nerve cells extended their neurites and survived for more than 3 weeks in vitro. Intracellular recording with a glass microelectrode revealed that, although their resting membrane potential was small, the tissue-cultured nerve cells recovered membrane excitability after continuous hyperpolarization by DC current passage. Both Na and Ca spikes were elicited in thus hyperpolarized nerve cells. Some nerve cells generated tetrodotoxin-resistant Na spikes.
D000200:Action Potentials / Q000187:drug effects; D000375:Aging; D000818:Animals; D002118:Calcium / Q000502:physiology; D046508:Culture Techniques / Q000379:methods; D004594:Electrophysiology; D005727:Ganglia, Spinal / Q000166:cytology; D008297:Male; D008564:Membrane Potentials; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D012964:Sodium / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology
D013779:Tetrodotoxin; D012964:Sodium; D002118:Calcium