A technique for collection and cultivation of macrophages from Cynomolgus monkeys (Macaca fascicularis).
Several techniques were compared for collection of peritoneal mononuclear phagocytes from cynomolgus monkeys. A multiple-holed cannula proved superior to a hypodermic needle for the collection of peritoneal washings because it was not occluded by the omentum. With the cannula technique 55--70% of the harvest medium could be recovered from the peritoneal cavity with a yield of 2.5--5 x 10(5) macrophages per ml. A closed system for injecting and collecting harvest medium was better than a syringe in preventing bacterial contamination of peritoneal washings. After collection, cells were resuspended in growth medium to a concentration of 5 x 10(5) macrophages per ml, distributed into Leighton tubes, and incubated at 36 degrees C. After 12 hours, cultures were washed to remove non-adherent cells and refed with growth medium. This technique provided a sterile, reliable method for the collection and cultivation of macrophages from monkeys.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002478:Cells, Cultured; D005260:Female; D008251:Macaca / Q000276:immunology*; D008252:Macaca fascicularis / Q000276:immunology*; D008264:Macrophages; D008297:Male; D009000:Monocytes / Q000032:analysis; D010586:Phagocytes / Q000032:analysis; D013048:Specimen Handling / Q000295:instrumentation / Q000662:veterinary*