title: [Evaluation of the adhesion, colonization and enterotoxigenicity of Vibrio cholerae in the enteral infection of intact and passively immunized newborn animals].
abstract: V. eltor were introduced into 323 suckling rabbits through a gastro-duodenal tube and into 629 suckling mice either by the same method, or per annum by a new specially developed technique. Passive immunization was achieved in suckling mice by the preliminary introduction of antibacterial sera into the small intestine or by feeding them with the milk of female mice immunized at the end of pregnancy, and in suckling rabbits by feeding them with the milk of goats immunized by introducing vibrios through the tests of the udder. To evaluate the results, the accumulation of liquid in the intestine and the development of diarrhea were taken into account, quantitative inoculation of homogenized intestinal matter was made; vibrios on the epithelium of the villi in the small intestine were counted and the changes of enterocytes and the lamina propria, observed in light and electron microscopy, were considered. The results of the complex quantitative evaluation in intact suckling animals revealed that the enterotoxigenicity of vibrios was manifested after their adhesion to and later colonization on the intestinal epithelium. Passive antibacterial immunization suppressed their adhesion and colonization, which resulted in protection from the enterotoxigenic effect of vibrios and their elimination from the intestine.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D002771:Cholera / Q000382:microbiology*; D002772:Cholera Toxin / Q000096:biosynthesis*; D007116:Immunization, Passive; D007410:Intestinal Diseases / Q000382:microbiology*; D007421:Intestine, Small / Q000382:microbiology; D051379:Mice; D011817:Rabbits; D014734:Vibrio cholerae / Q000472:pathogenicity*
chemical_list: D002772:Cholera Toxin
vernacular_title: Ostenka adgezii, kolonizatsii i énterotoksigennosti kholernykh vibrionov pri énteral'nom zarazhenii intaktnykh i passivno immunizirovannykh novorozhdennykh zhivotnykh.