Survival of cow blastocysts utilizing short freezing curves.
Blastocysts can survive direct transfer into liquid nitrogen after programmed freezing in 1.4 M glycerol to -29 degrees C when thawed by direct transfer into water at 37 degrees C. This observation facilitates the use of deep freezing of blastocysts in embryo transfer procedures. This makes the requirement for a special apparatus for the freezing procedure less demanding.
D000818:Animals; D001757:Blastomeres; D002417:Cattle / Q000502:physiology*; D004624:Embryo Transfer / Q000662:veterinary; D005260:Female; D005615:Freezing; D005990:Glycerol; D011247:Pregnancy; D011270:Pregnancy, Animal; D014021:Tissue Preservation / Q000379:methods / Q000662:veterinary*
D005990:Glycerol