The effects of administration of semicarbazide and aminooxyacetic acid on B6 vitamer levels in subcellular fractions of mouse brain.
The in vivo effects of semicarbazide (SC) and aminooxyacetic acid (AOAA) on the B6 vitamer concentrations in brain subcellular fractions, especially in extrasynaptosomal, synaptosomal plasma, extrasynaptosomal and synaptosomal mitochondria, were investigated. Treatment of mice with SC decreased levels of pyridoxal phosphate (PLP) in extrasynaptosomal and synaptosomal plasma, while it had no effect on those in extrasynaptosomal and synaptosomal mitochondria. On the contrary, treatment with AOAA more significantly decreased pyridoxamine phosphate (PMP) levels in extrasynaptosomal and synaptosomal mitochondria than those in extrasynaptosomal and synaptosomal plasma. According to these results, the difference between in vivo effects of SC and AOAA on vitamin B6-enzymes was discussed.
D000085:Acetates / Q000494:pharmacology*; D000625:Aminooxyacetic Acid / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000648:ultrastructure*; D004347:Drug Interactions; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000378:metabolism; D011733:Pyridoxamine / Q000031:analogs & derivatives / Q000378:metabolism; D011736:Pyridoxine / Q000378:metabolism*; D012663:Semicarbazides / Q000494:pharmacology*; D013347:Subcellular Fractions / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism
D000085:Acetates; D009943:Organophosphorus Compounds; D012663:Semicarbazides; D000625:Aminooxyacetic Acid; D011732:Pyridoxal Phosphate; D011733:Pyridoxamine; D011736:Pyridoxine; C010627:pyridoxamine phosphate