Polymerization of Escherichia coli bacteriophage T4D short tail fibers into filaments and filament bundles.
Crude preparations of short tail fibers of E. coli T4D bacteriophage were found to be readily soluble in 0.01 M EDTA, but after dialysis against H2O, and a 10-fold concentration, the fibers aggregated into long filaments and filament bundles. However, since the filaments from these crude preparations were associated with nonfilamentous material, the fibers were purified in the presence of 0.01 M EDTA. After dialysis of the purified short tail fiber preparation against water, and 10-fold concentration, a precipitate was again formed that could be readily separated by low speed centrifugation. In micrographs, this precipitated material showed large aggregates of short tail fibers but did not show well-defined filaments. However, when these aggregates were incubated with 0.02 M MgSO4 and 0.06 M KC1, well-defined filaments were again formed.
D004591:Electrophoresis, Polyacrylamide Gel; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D011485:Protein Binding; D013604:T-Phages / Q000648:ultrastructure*; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
D046911:Macromolecular Substances; D014764:Viral Proteins