title: [Practical application of histochemical methods in lung diseases].
abstract: A short review of different applications of enzyme-histochemistry and enzyme-cytochemistry in the field of lung pathology is given. As shown in own experiments, the phosphomolybdic-acid-stannous-chloride reaction (Landing et al. 1952) appears as a successful method for rapid demonstration of phospholipids in histological and cytological material. Application of histochemistry (reaction of acid phosphatase) in diagnostic of sarcoidosis (Kveim-test) gives in comparison with the traditional technique following advantages: 1. results are obtained more rapidly and with minor technical expense, 2. for pathologists, it is easier to differentiate between specific and non-specific reactions. With cytochemical methods (reaction of alkaline phosphatase) is is relatively easy to detect tumour cells in pleural fluids and ascites. This test (Eckert 1978, 1979) is neither cancer-specific nor does it cover all tumours cases. But its high positivity and its easy readability enable the technique to be used as a search method for tumour cells.
mesh_terms: D000469:Alkaline Phosphatase; D003937:Diagnosis, Differential; D006651:Histocytochemistry; D006801:Humans; D008168:Lung / Q000032:analysis; D008171:Lung Diseases / Q000175:diagnosis*; D008175:Lung Neoplasms / Q000175:diagnosis / Q000378:metabolism; D008982:Molybdenum / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010756:Phosphoric Acids / Q000032:analysis; D012507:Sarcoidosis / Q000175:diagnosis
chemical_list: D010743:Phospholipids; D010756:Phosphoric Acids; D008982:Molybdenum; D000469:Alkaline Phosphatase; C003125:phosphomolybdic acid
vernacular_title: Praxisbezogene Anwendung histochemischer Methoden in der Lungenheilkunde.