Studies on nitrosamine formation by the interaction between drugs and nitrite. I. Measurement of the amount of nitrosamine formed in rat and guinea pig stomachs.
The amounts of nitrosamine formed by the interaction of several drugs and nitrite in rat and guinea pig stomachs were estimated. The nitrosamine formation from aminopyrine and nitrite was much more in guinea pig stomachs than in rat stomachs. It seemed that this result was due to the difference in gastric contents of these animals. As the nitrosamine formation was also indicated in the interaction of a clinically prescribed dose of aminopyrine and very low doses of nitrite in rat and guinea pig stomachs, there is a possibility that the nitrosation of aminopyrine takes place in human stomachs, too. Minocycline also interacted with nitrite and formed N-nitrosodimethylamine. However, the amount of nitrosamine formed was less in minocycline than in aminopyrine. Oxytetracycline was not found to form nitrosamine in the stomachs of these animals. The effects of several compounds on the nitrosamine formation by the interaction of aminopyrine and nitrite were also investigated. Ascorbic acid, sodium erythorbate, propyl gallate and butylated hydroxyanisole reduced the nitrosamine formation but the effect of alpha tocopherol, sorbic acid and butylated hydroxytoluene was not observed in the stomach.
D000632:Aminopyrine / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D004347:Drug Interactions; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D006168:Guinea Pigs; D008297:Male; D008911:Minocycline / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism*; D009602:Nitrosamines / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D000975:Antioxidants; D009573:Nitrites; D009602:Nitrosamines; D000632:Aminopyrine; D008911:Minocycline