Spectroscopic study of the dar interaction and of the photoreaction between a new monofunctional psoralen: 3-carbethoxy psoralen, and DNA.
3-carbethoxypsoralen (3-CPs) is a new linear psoralen derivative. Its dark interaction and photoreaction with DNA has been studied and compared with that of a well known bifunctional psoralen : 8-methoxypsoralen (8-MOP). 3-CPs is able to form in the dark a non covalent complex with native DNA. After irradiation of this complex with UV-A light (365 nm) 3-CPs is able to link covalently to DNA. Heat denaturation and renaturation patterns of treated DNA clearly show that, in contrast to 8-MOP, 3-CPs does not form DNA interstrand cross links. Fluorescence studies show that the photobinding of 3-CPs gives rise to the formation of monoadducts involving the 4',5' double bond of this molecule.
D000818:Animals; D004247:DNA; D003624:Darkness; D011564:Furocoumarins; D066298:In Vitro Techniques; D008297:Male; D008730:Methoxsalen; D009691:Nucleic Acid Denaturation; D010777:Photochemistry; D012474:Salmon; D013050:Spectrometry, Fluorescence / Q000379:methods; D013094:Spermatozoa / Q000032:analysis; D014466:Ultraviolet Rays
D011564:Furocoumarins; D004247:DNA; C017104:3-carbethoxypsoralen; D008730:Methoxsalen