Response of the isolated rabbit ciliary epithelium to adrenergic drugs following superior cervical ganglionectomy.
The fluid permeability of the isolated ciliary epithelium of the rabbit eye to adrenergic agonist's has been measured in paired normal and ganglionectomized tissues. The agonists norepinephrine (NE), epinephrine (E), phenylephrine (P) and isoproterenol (ISO) were used at concentrations from 10(-12) to 10(6)M in the presence and absence of cocaine 2.6 or 26x10(-6)M (C, to prevent uptake of agonist in the synapse); iproniazid (I) 3.6 x 10(-5)M, or clorgyline (CL) 5x10(-5)M (to inhibit 10(-5)M, or clorgyline (CL) 5x10(-5)M (to inhibit monoamine oxidase); dibenamine, (D) 1x10(-5) (to block extraneuronal uptake) and/or 1x10(-8)M phenoxybenzamine (PH) (an alpha-adrenergic antagonist). No supersensitivity to adrenergic agonists was found in ganglionectomized tissues. Normal tissues showed an increased fluid permeability after agonist alone with the other of efficacy at 10(-8)M being ISO greater than NE greater than E greater than P, while in ganglionectomized tissues the order at 10(-8)M was E = NE greater than ISO = P. Normal tissues showed a sensitivity to agonists at 10(-8)M in order E greater than NE greater than P greater than ISO after treatment with C, I and D, while ganglionectomized tissues revealed a sensitivity to NE greater than P greater than ISO greater than E, even though the quantitative response after C, I and D was reduced. It appears, therefore, that both alpha- and beta-receptors exist in the ciliary epithelium, and although ganglionectomy alters the tissues responsiveness to different adrenergic agonists it is not the site of the supersensitivity seen in the whole eye.
D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D002924:Ciliary Body / Q000187:drug effects* / Q000294:innervation / Q000378:metabolism; D003010:Clorgyline / Q000494:pharmacology; D003042:Cocaine / Q000494:pharmacology; D003983:Dibenzylchlorethamine / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects; D005123:Eye / Q000378:metabolism; D005726:Ganglia, Parasympathetic / Q000601:surgery*; D007490:Iproniazid / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D010643:Phenoxybenzamine / Q000494:pharmacology; D010656:Phenylephrine / Q000494:pharmacology; D011817:Rabbits; D013566:Sympathomimetics / Q000494:pharmacology*
D013566:Sympathomimetics; D010643:Phenoxybenzamine; D010656:Phenylephrine; D003983:Dibenzylchlorethamine; D007490:Iproniazid; D003042:Cocaine; D007545:Isoproterenol; D003010:Clorgyline; D009638:Norepinephrine; D004837:Epinephrine