Translation in vitro of ribonucleic acids from potato virus X, potato virus M and white clover mosaic virus.
Ribonucleic acids from three plant viruses: potato virus X (PVX), potato virus M (PVM) and white clover mosaic virus (WClMV) have been purified and used as messengers in a wheat germ cell-free system. It was demonstrated that each of these viruses contained heavy genomic RNA species of mol. wt. about 2 x 10(6), which appeared to be very efficient templates for protein synthesis in vitro. The synthesis of high-molecular-weight polypeptides: 180 x 10(3), 190 x 10(3) and 170 x 10(3) was directed by PVX, PVM and WClMV RNAs, respectively. None of the polypeptides directed by any of the RNAs studied corresponded to the coat proteins.
D008970:Molecular Weight; D009029:Mosaic Viruses / Q000378:metabolism; D010452:Peptide Biosynthesis; D010942:Plant Viruses / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis / Q000378:metabolism*
D012333:RNA, Messenger; D012367:RNA, Viral