[Biological applications of Raman resonance spectroscopy and micro-Raman resonance spectroscopy].
Advantages are given to illustrate the possibilities of resonance Raman spectroscopy in the studies of biological systems. A relation giving the size of porphinato-core of metalloporphyrins from resonance Raman frequency is proposed. It can be applied to heme, even in lived state. By illuminating the sample through a microscope, resonance Raman effect can be collected from 1 (micrometer)2 of sample. Spectra of a vegetal-cell wall (pimento) and of a red corpuscle (hemoglobin) are shown as examples.
D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D008665:Metalloporphyrins / Q000032:analysis; D010945:Plants, Edible / Q000032:analysis; D013059:Spectrum Analysis, Raman / Q000379:methods*
D006454:Hemoglobins; D008665:Metalloporphyrins
Applications biologiques de la spectroscopie Raman de résonance et de la spectroscopie micro-Raman de résonance.