High-resolution protein mapping of human fibroblasts and hair root cells: a standardized reproducible procedure considering the effect of cell culture parameters.
The effect of different cell culture parameters on two-dimensional polypeptide maps of human fibroblasts is considered. Improved culture methods are introduced to get better resolution and reproducibility. Based on these investigations, highly standardized techniques for the protein mapping of this tissue and also of hair root cell lysates are presented. These methods seem to be quite suitable for analysis of cellular synthesized proteins and study of variability in normal subjects and in patients with genetic disorders.
D002874:Chromosome Mapping; D003470:Culture Media / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism*; D006197:Hair / Q000378:metabolism*; D006801:Humans; D011506:Proteins / Q000235:genetics*
D003470:Culture Media; D011506:Proteins