Identification of nematode larvae by coal cultures in closed boxes.
A method is described for the identification of concentrated larvae from coal cultures in closed boxes. The condensation drops formed beneath the lid of the culture box are taken up with an L-shaped pipette. The sample taken up is added to a drop of Lugol's solution on a slide and examined under the microscope. The details of the nematode structure are clearly visible. Great care should be taken to avoid a possible cutaneous contamination.
D000818:Animals; D003031:Coal; D003470:Culture Media; D007814:Larva / Q000033:anatomy & histology / Q000302:isolation & purification; D008722:Methods; D009348:Nematoda / Q000033:anatomy & histology / Q000302:isolation & purification*
D003031:Coal; D003470:Culture Media