Bioluminescent assay for determination of picomole amounts of AMP, ADP, and ATP in adipose and muscle tissue biopsies.
A method is described for quantitative determinations of AMP, ADP, and ATP in tissue samples weighing a few milligrams by utilizing constant bioluminescent light intensity after reagent mixing. The smallest measurable quantity of adenosine nucleotide amounts to 0.2 pmol (coefficient of variation = 0.05) in the bioluminescent assay.
D000227:Adenine Nucleotides / Q000032:analysis*; D000244:Adenosine Diphosphate / Q000032:analysis; D000249:Adenosine Monophosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D000273:Adipose Tissue / Q000032:analysis*; D049454:Firefly Luciferin; D006801:Humans; D008156:Luciferases; D008832:Microchemistry; D009132:Muscles / Q000032:analysis*
D000227:Adenine Nucleotides; D000249:Adenosine Monophosphate; D049454:Firefly Luciferin; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D008156:Luciferases