Production of R-type virus-like particles in hamster cells.
The production and release of R-type virus-like particles (VLP) was studied in several Syrian hamster cell lines. Most but not all hamster cell lines contain detectable levels of R-type VLP. However, only BHK-21 clone F (CF) cells would release the VLP into culture fluids. Treatment with dexamethasone enhanced to a limited extent the production and release of VLP from BHK-21 CF cells. Actinomycin D inhibited the production and release of R-type VLP in hamster cells, suggesting that some transcription from DNA is necessary for VLP production. Furthermore, 5-iododeoxyuridine induced the production of R-type VLP in Syrian hamster embryo cells. These results suggest that R-type VLP are endogenous to Syrian hamsters.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003609:Dactinomycin / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D007065:Idoxuridine / Q000494:pharmacology; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure*; D007668:Kidney; D008647:Mesocricetus; D012328:RNA Viruses / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis; D014775:Virus Activation
D012367:RNA, Viral; D003609:Dactinomycin; D003907:Dexamethasone; D007065:Idoxuridine