[Micronucleus formation compared to the survival rate of human melanoma cells after X-ray and neutron irradiation and hyperthermia].
After neutron and X-ray irradiation and combined X-ray irradiation and hyperthermia (3 hours, 42 degrees C), the survival rate of human melanoma cells was measured by means of the colony formation test and compared to the formation of micronuclei. Neutrons had a stronger effect on the formation of micronuclei than the combination of X-rays and hyperthermia. X-rays had the lowest effect. The dose effect curve showed a break at that dose level at which a reduction of cells was observed in the cultures. A good relation between survival rate and formation of micronuclei was found for the X-ray irradiation, but not for the neutron irradiation and the combined treatment. These observations are discussed. At least for X-rays, the micronucleus test has turned out to be a good screening method for the radiosensitivity of a biologic system.
D002460:Cell Line; D002467:Cell Nucleus / Q000528:radiation effects; D003114:Colony-Forming Units Assay; D004307:Dose-Response Relationship, Radiation; D005334:Fever; D006801:Humans; D008545:Melanoma / Q000532:radiotherapy*; D009502:Neutrons
Mikronukleusbildung im Vergleich zur Uberlebensrate von menschlichen Melanomzellen nach Röntgen-, Neutronenbestrahlung und Hyperthermie.