title: [Relationship between structure and function of a chemotactic factor for human leukocytes].
abstract: Casein, a phosphoprotein forming aggregates in solution, exerts chemotactic activity for human neutrophils. The protein was filtered on Sephadex G 100 to obtain fractions of homogeneous mol. weight and the column fractions were tested for chemotactic activity. The chemotactic activity was found only in the lighter peak, which was then digested by trypsin. The digested material was purified by filtration through AcA-54 and the 6.000 M.W. peptide containing the most phosphate groups and tyrosines resulted chemotactic for PMN. Most likely the phosphate groups are important in chemotactic recognition.
mesh_terms: D002364:Caseins / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D002850:Chromatography, Gel; D006801:Humans; D008970:Molecular Weight; D009504:Neutrophils / Q000187:drug effects; D013329:Structure-Activity Relationship; D014357:Trypsin / Q000378:metabolism
chemical_list: D002364:Caseins; D014357:Trypsin
vernacular_title: Relazione tra struttura e funzione di un fattore chemiotattico per i leucociti umani.