[Nuclear RNP containing pre-mRNA. 16. Cross-linked informofers].
The proteins of 30S RNP particles containing pre-mRNA (hnRNA) were cross-linked with bifunctional reagents (dimethylsuberimidate and dimethyl-3,3'dithiobispropionimi-date). Further treatment with 1 or 2 M NaCl dissociates all RNA from protein. However, a significant part of protein particles--informofers, being cross-linked survived high salt treatment. Their sedimentation coefficients were close to those of original particles. No RNA could be detected in the informofers even after labeling the cells with a precursor for a long period of time. Sodium dodecylsulfate or urea dissociated cross-linked informofers into oligomeric polypeptides. They could be dissociated by beta-mercaptoethanol treatment if a reversible cross-linking reagent has been used. The resulting polypeptides were represented by informatin. RNP particles (30S RNP or polyparticles) were reconstituted upon mixing or cross-linked informofers with pre-mRNA and removal of 2 M NaCl. The reconstituted particles were indistinguishable from the original ones by several tests.
D002708:Chlorella / Q000032:analysis; D003432:Cross-Linking Reagents; D004120:Dimethyl Suberimidate; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D007096:Imidoesters; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins; D009994:Osmolar Concentration; D011487:Protein Conformation; D012333:RNA, Messenger / Q000235:genetics; D012261:Ribonucleoproteins
D003432:Cross-Linking Reagents; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D007096:Imidoesters; D009698:Nucleoproteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D004120:Dimethyl Suberimidate; C007471:dimethyl dithiobispropionimidate
Iadernye RNP, Soderszhashcie pre-mRNK. XVI. "Zashitye" informofery.