In vitro study of complement dependent active adherence on SLE and RA leukocytes.
Immunological disorders may cause altered motility or attachment of leukocytes. Active adherence of buffy cost cells derived from normal subjects and with autoimmune diseases patients and complement dependency of this process was studied on glass surfaces. The adherence of leukocytes of both normal persons and of SLE and RA patients was decreased in the presence of normal human serum compared to serum-free medium. The effects of native and heat inactivated human sera on leukocytes of SLE and RA patients were compared. Adherence of SLE leukocytes was higher, whereas that of RA leukocytes was lower in unheated serum compared to heat inactivated human serum. Supernatants of leukocytes of SLE, RA patients and control subjects were harvested after incubation in serum-free medium. The C3 levels of supernatants were determined by rocket electrophoresis. Surface attached C3 was detected with immunofluorescence mainly on SLE leukocytes. The cellular effects of supernatants on control leukocytes, were consistent with the changes observed with leukocytes derived from SLE, RA or control patients.
D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001769:Blood; D002448:Cell Adhesion; D003176:Complement C3 / Q000032:analysis / Q000378:metabolism; D003165:Complement System Proteins / Q000378:metabolism*; D003470:Culture Media; D006801:Humans; D007957:Leukocyte Adherence Inhibition Test; D007962:Leukocytes / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D011951:Receptors, Complement
D003176:Complement C3; D003470:Culture Media; D011951:Receptors, Complement; D003165:Complement System Proteins