[Effect of a protein-deficient diet (5 p. 100 gluten) and of balanced refeeding (15 p. 100 casein) on potential lipase, colipase-dependent lipase and phospholipase A2 activities. II. In the pancreatic juice of the growing rat].
We experimented with a diet resembling a protein-deficient one eaten by man, having a low protein level and proteins of poor biological value. Malnutrition caused weight loss accompanied by an overall reduction of ingesta. However, the food intake, as compared to animal weight, was the same in the undernourished animals as in the controls. Bile and pancreatic juice outputs were not altered continuously and regularly but with the poor protein diet, they were lower. Refeeding a balanced diet caused these outputs to increase. When 0.6 p. 100 of methionine was added to the deficient diet, no significant difference was noted in the outputs of the rats eating the deficient diets. Enzyme activities in the juice and pancreas varied widely from one day to another during malnutrition and during refeeding. The mean values of specific activities, which masked these variations, showed that malnutrition did not significantly decrease the phospholipase A2 activity in the pancreatic juice, while potential lipase and colipase-dependent lipase activities declined. Refeeding temporarily stimulated specific phospholipase A2 and potential lipase activities the third day; colipase-dependent lipase activity was not activated. After 2 to 3 weeks of refeeding, the specific activities of the three enzymes were similar to the control values, but were no longer so at the end of refeeding. This would suggest a return to the normal, as shown by the oscillations around the control values. Protein malnutrition, as refeeding, did not in general cause the same changes in all the enzymes at the same time.
D000818:Animals; D001646:Bile / Q000378:metabolism; D001835:Body Weight; D003089:Colipases / Q000494:pharmacology*; D004044:Dietary Proteins / Q000494:pharmacology*; D004435:Eating; D008049:Lipase / Q000378:metabolism*; D008297:Male; D010179:Pancreas / Q000254:growth & development; D010189:Pancreatic Juice / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011488:Protein Deficiency / Q000201:enzymology*; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D003089:Colipases; D004044:Dietary Proteins; D011506:Proteins; D010740:Phospholipases; D008049:Lipase; D010741:Phospholipases A; D054467:Phospholipases A2
Influence d'un régime de malnutrition protéique (5 p. 100 de gluten) et d'une réalimentation équilibrée (15 p. 100 de caséine) sur les activités de la lipase potentielle, de la lipase-colipase dépendante et de la phospholipase A2. II. Suc pancréatique de rat en croissance.