Antioxidant effect of cupric complex on NADPH-dependent lipid peroxidation in rat liver microsomes.
The antioxidant effect of His-Cu (Histidine and cupric ion) on NADPH-dependent lipid peroxidation was proportional to the inhibitory effect on cytochrome c reduction i the presence of microsomes and ADP-Fe (ADP and ferric ion). The inhibiton of cytochrome c reduction by His-Cu caused no inhibition of NADPH oxidation. ADP-Fe stimulated NADPH oxidation in the absence of cytochrome c, and inhibited cytochrome c reduction. His-Cu inhibited NADPH-oxidation stimulated by ADP-Fe, but the antioxidant effect of His-Cu was independent of the effect on cytochrome b5 reduction. These results suggest that the antioxidant effect of His-Cu depends on the inhibitory effect on the electron transport from NADPH-cytochrome c reductase to ADP-Fe, but not on the inhibitory effect on the activity of the reductase.
D000818:Animals; D000975:Antioxidants; D003300:Copper / Q000494:pharmacology*; D006639:Histidine / Q000494:pharmacology*; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D011919:Rats, Inbred Strains; D014812:Vitamin K
D000975:Antioxidants; D008054:Lipid Peroxides; D014812:Vitamin K; D006639:Histidine; D009249:NADP; D003300:Copper