Glutathione and glutathione S-transferase activities of mammalian cells in culture.
Continuous cell cultures derived from various tissues of rat, mouse, hamster and man were assayed for their glutathione (GSH) content and glutathione S-transferase activities. GSH S-transferase activities were monitored toward the substrates 1-chloro-2,4-dinitro-benzene (CDNB), 1,2-dichloro-4-nitrobenzene (DCNB) and 1,2-epoxy-3-(p-nitrophenoxy)propane (PO). All cell lines tested contained appreciable amounts of GSH ranging from 10 to 65 nmol/mg cellular protein. Likewise, all cell lines expressed GSH S-transferase activities. However, the various cell lines differed considerably in their relative transferase activities exhibiting some degree of species-specificity.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004137:Dinitrochlorobenzene; D004852:Epoxy Compounds; D005978:Glutathione / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009578:Nitrobenzenes; D009596:Nitrophenols; D051381:Rats; D013045:Species Specificity; D013379:Substrate Specificity
D004137:Dinitrochlorobenzene; D004852:Epoxy Compounds; D009578:Nitrobenzenes; D009596:Nitrophenols; C007606:1,2-epoxy-3-(p-nitrophenoxy)propane; C028328:1,2-dichloro-4-nitrobenzene; D005982:Glutathione Transferase; D005978:Glutathione