[Elimination of radiation-induced chromosomal damages in a human peripheral lymphocyte culture. I. The frequency of aberrations in the 1st and 2d mitoses].
A comparative analysis of chromosome aberrations in the first and second division of cells in human peripheral blood lymphocyte cultures after gamma-irradiation in vitro at 1--5 Gy doses was performed. The lymphocytes of irradiated blood were incubated at 37 degrees with PHA and BrdU (20 micrograms/ml) for 58--66 hours. The first, second and third postirradiation division cells were identified by the sister chromatid differential staining technique. The percentage of cells in the first, second and third mitoses varied from 32 to 77, from 23 to 68 and from 0 to 9, respectively. Essential differences in frequency of aberrant cells and all chromosome-type aberrations between the first and second mitoses have been found at all irradiation doses. The frequency of paired fragments and dicentrics in the first division cells was on the average, respectively, 1.6 and 2 times higher than in the second division cells. In the first division cells almost all dicentrics were with associated paired fragments, in the second division cells the frequency of dicentrics without fragment amounted to 25--50%. At the range--doses of 2 to 5 Gy the distribution of dicentrics between cells in the first and second mitosis did not differ from the Poisson one.
D000328:Adult; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D005260:Female; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects*; D008297:Male; D008938:Mitosis / Q000528:radiation effects*; D011336:Probability; D012854:Sister Chromatid Exchange / Q000528:radiation effects
Eliminatsiia radiatsionno-indutsirovannykh povrezhdeniĭ khromosom v kul'ture limfotsitov perifericheskoĭ krovi cheloveka. I. Chastota aberratsiĭ v pervom i vtorom mitoze.