The role of visceral epithelial, endothelial and mesangial cells in the formation and turnover of glomerular basement membrane.
Incorporation of 3H-proline in the mouse kidney was studied by electron microscopic radioautography in a pulse-chase mode to establish the role of visceral epithelial, endothelial, and mesangial elements in the synthesis and turnover of glomerular basement membrane. Visceral epithelial and endothelial cells were found to play a significant role in the formation of glomerular basement membrane components, and turnover time for one of the components was found to be under 2 h. The synthesis of second component is slow and make take 8-24 h. The mesangial cells appear to play a significant role in the reabsorption of the component with a faster turnover rate.
D000818:Animals; D001345:Autoradiography; D001485:Basement Membrane / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D004727:Endothelium / Q000502:physiology; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D007668:Kidney / Q000502:physiology* / Q000648:ultrastructure; D007678:Kidney Glomerulus / Q000098:blood supply / Q000502:physiology* / Q000648:ultrastructure; D046911:Macromolecular Substances; D051379:Mice; D008854:Microscopy, Electron; D011392:Proline / Q000378:metabolism / Q000494:pharmacology; D013997:Time Factors
D046911:Macromolecular Substances; D011392:Proline