The induction of rat lung aryl hydrocarbon hydroxylase by diluted smoke from commercial cigarettes.
Induction of pulmonary aryl hydrocarbon hydroxylase (AHH) by cigarette smoke in small rodents is well established. However, studies on dose-response relationships particularly at the lower ends of such relationships are few indeed. Availability of the British-American Tobacco Co. (B.-A.T.)-Mason inhalation system, in which precisely-calibrated dilutions of cigarette smoke can be released into an inhalation chamber, enables excellent dose-response relationships to be established. Using this inhalation system and cigarettes delivering tar (total particulate matter, water and nicotine free) equal to 1, 4 and 20 mg/cigarette, it has been shown that induction of AHH in male Sprague-Dawley rat lung is an extremely sensitive system to inhaled cigarette smoke. AHH induction was observed at a 200-s exposure to 40 puffs of a 1 : 5 dilution of smoke from an ultramild cigarette delivering 1 mg tar. At this low concentration, it is not even possible to accurately weight the TPM from diluted smoke in the exposure chamber. This extremely sensitive rat pulmonary enzyme system may prove valuable in the biological testing of modern low tar cigarettes.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis*; D004790:Enzyme Induction; D008168:Lung / Q000201:enzymology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012906:Smoke / Q000032:analysis; D012907:Smoking
D012906:Smoke; D001189:Aryl Hydrocarbon Hydroxylases