Formation of N-2-fluorenylhydroxylamine adducts of DNA in vivo and in vitro and some of their properties.
The major 2-fluorenylamine-DNA derivative formed in vivo in rat liver after application of N-2-flourencylacetamide is N-(deoxyguanosin-8-yl)-2-fluorenylamine. This nucleoside, hydrolyzed under mild alkaline conditions with the opening of the imidazole ring, formed two pyrimidine derivatives which can be separated by Sephadex LH-20 column chromatography and thin-layer chromatography on silica. The hydrolysis reaction was catalyzed by metal ions and alkaline phosphatase from Escherichia coli.
D015073:2-Acetylaminofluorene / Q000378:metabolism*; D000818:Animals; D001119:Arginase / Q000378:metabolism; D001711:Biotransformation; D004247:DNA / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D004247:DNA; D015073:2-Acetylaminofluorene; D001119:Arginase