Procedure for granulokinetic studies in the horse with chromium-51.
A procedure with chromium-51 (51Cr) as the cell label that maintains high-cell viability for studying granulocyte kinetics in horses is described. The procedure combines and modifies several methods for isolating leukocytes and granulocytes for use in the horse when a large volume of labeled cells is required. Also described is an improved technique for measuring granulocyte specific activity in large serial blood samples, using a Ficoll-sedimentation method. The procedure should be useful for determining granulocyte kinetics in the horse, the only major domestic species for which such data are not available.
D000818:Animals; D002469:Cell Separation / Q000662:veterinary*; D002860:Chromium Radioisotopes; D004912:Erythrocytes / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism; D006736:Horses / Q000097:blood* / Q000378:metabolism; D007700:Kinetics; D007962:Leukocytes / Q000378:metabolism*
D002860:Chromium Radioisotopes