A RAST assay for monitoring specific IgE antibodies in the mouse.
Mouse monoclonal anti-ovalbumin IgE was isolated from serum of BALB/c mice implanted with the IgE secreting hybridoma (14-205). Purified IgE was used to immunize rabbits and for the preparation of an antiserum specific for mouse IgE. The latter was used to develop a RAST assay to replace the PCA technique in monitoring antigen-specific IgE response in the mouse.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000918:Antibody Specificity; D005260:Female; D007073:Immunoglobulin E / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis; D011852:Radioallergosorbent Test; D012016:Reference Values
D000911:Antibodies, Monoclonal; D007073:Immunoglobulin E