Investigation of alveolar macrophage function using lucigenin-dependent chemiluminescence.
The method using lucigenin-dependent phagocytic chemiluminescence is described for the assessment of alveolar macrophage metabolic activity in response to stimulation by opsonised particles or soluble agents. The requirement for superoxide anion (O2-) in the production of chemiluminescence is suggested by inhibition (95%) using superoxide dismutase. The results obtained are correlated with those obtained using another method of detecting O2- release (r = 0.61; p less than 0.05) and are also related by regression analysis to polymorphonuclear leucocyte contamination of the alveolar macrophage suspension. This shows that alveolar macrophages produce lucigenin-dependent chemiluminescence of the same order of magnitude as do polymorphonuclear leucocytes.
D000166:Acridines / Q000378:metabolism*; D006801:Humans; D008163:Luminescent Measurements; D008264:Macrophages / Q000378:metabolism*; D009504:Neutrophils; D011650:Pulmonary Alveoli / Q000378:metabolism*; D007507:Therapeutic Irrigation
D000166:Acridines; C033472:10,10'-dimethyl-9,9'-biacridinium