Flow cytometric analysis of the effect of ara-C on the chronobiology of bone marrow DNA synthesis.
Flow cytometry, a technique which allows rapid quantitation of the percentages of a cell population in the various cell cycle phases, closely duplicated the previously reported circadian rhythm for mouse bone marrow DNA synthesis. The effect of ara-C, an S phase specific anti-neoplastic drug, on this rhythm was investigated. Ara-C was shown to suppress this rhythm to a much greater degree when injected at the peak of the bone marrow DNA synthesis as compared to the low. Thus, damage to the bone marrow during treatment with ara-C may be reduced if the circadian rhythm of bone marrow DNA synthesis is taken into account.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism; D002940:Circadian Rhythm / Q000187:drug effects*; D003561:Cytarabine / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D005434:Flow Cytometry; D009115:Muridae
D003561:Cytarabine; D004247:DNA