[Interpretation of results on a human basophil degranulation test (author's transl)].
A statistical analysis of a result on a human basophile degranulation test was performed. The shape of the curve representing the number of basophiles, as a function of the logarithm of the allergenic dosage, was parabolic, in the case of degranulation. This curve permits a calculation of the dosage necessary to provoke a degranulation of 10 per cent, in comparison with the mean of the two control test tubes. The authors also determined the significant degranulation threshold to be 5 per cent, as regards the mean number of cells counted for the controls (example: for 50 control cells counted, the threshold was equal to 28 per cent degranulation). This statistical analysis largely facilitates the interpretation of the granulation test by basophiles, by assuring the value of observed degranulations, and by allowing a better quantification of the phenomenon. The latter might permit a comparison of different batches of allergen, that is, the standardisation of allergens. These calculations can be easily programmed, using a currently available calculator.
D001491:Basophils / Q000276:immunology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis*; D011336:Probability
Interprétation du résultat d'un test de dégranulation des basophiles humains.