Role of virus strain in conventional and enhanced measles plaque neutralization test.
A low-passage Edmonston strain of measles virus gave on the average four-fold higher antibody titers than two high-passage strains of Edmonston virus when used in a virus plaque neutralization test. Heterologous anti-human immunoglobulin did not affect antibody titers obtained with the low-passage virus but enhanced antibody titers obtained with the high-passage viruses on the average eight-fold. These differences in virus sensitivity to neutralization and neutralization potentiation by anti-immunoglobulin were interpreted as being possibly due to differences in density or accessibility of the functional antigens of measles virus. The conventional plaque neutralization test utilizing the low-passage Edmonston virus was about 10 times more sensitive than the virus CPE-neutralization test, 60 times more sensitive than the measles hemagglutination-inhibition test and 220 times more sensitive than the measles complement-fixation antibody test.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002460:Cell Line; D002522:Chlorocebus aethiops; D003168:Complement Fixation Tests; D003588:Cytopathogenic Effect, Viral; D006385:Hemagglutination Inhibition Tests; D007668:Kidney; D008459:Measles virus / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests; D010948:Viral Plaque Assay
D000914:Antibodies, Viral