title: The effect of temperature and antimetabolites on antibody binding to the outer surface of second stage Toxocara canis larvae.
abstract: In vitro maintained second stage Toxocara canis larvae do not bind antiserum raised to their excretions and secretions (ES) at 37 degrees C as detected by indirect fluorescence. However, when these larvae were incubated at 2 degrees C under the same conditions intense fluorescence on the whole outer surface was observed. This fluorescence remained as long as the larvae were maintained at 2 degrees C. When these larvae were reincubated at 37 degrees C a gradual loss of fluorescence along their outer surfaces occurred. This loss was complete after 3 h. Larvae which were preincubated in antimetabolites at 37 degrees C exhibited intense fluorescence on their outer surfaces as did those incubated at 2 degrees C with antimetabolites. It is concluded that antigens present in ES occur along the whole length of the larval outer surface and turn over at 37 degrees C. This turnover occurs along the whole outer surface and is metabolically dependent. Should this occur in vivo it could afford the parasite with a mechanism for evasion of the immune response.
mesh_terms: D019297:2,4-Dinitrophenol; D000818:Animals; D000937:Antigen-Antibody Reactions / Q000187:drug effects*; D000954:Antigens, Surface / Q000276:immunology*; D000963:Antimetabolites / Q000494:pharmacology*; D001386:Azides / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D004285:Dogs; D005260:Female; D007460:Iodoacetamide / Q000494:pharmacology; D007814:Larva / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D019810:Sodium Azide; D012969:Sodium Fluoride / Q000494:pharmacology; D013696:Temperature; D014119:Toxocara / Q000187:drug effects / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D000963:Antimetabolites; D001386:Azides; D004140:Dinitrophenols; D012969:Sodium Fluoride; D019810:Sodium Azide; D019297:2,4-Dinitrophenol; D007460:Iodoacetamide