title: RNA synthesis in isolated yeast mitochondria.
abstract: Isolated yeast mitochondria incorporate added UTP into RNA. Amongst the products formed are the two rRNAs, 4S RNA and several components presumed to be mRNAs. In omega+ strains (containing an intervening sequence in the 21S rRNA gene) besides mature 21S rRNA a transcript could be detected still containing nucleotide sequences transcribed from this intervening sequence. In omega- strains (not containing this intervening sequence) also a longer form of the 21S rRNA could be observed. These results suggest that isolated yeast mitochondria are capable of carrying out RNA synthesis and processing, including splicing.
mesh_terms: D004272:DNA, Mitochondrial / Q000235:genetics*; D003609:Dactinomycin / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012331:RNA, Fungal / Q000096:biosynthesis* / Q000235:genetics; D012440:Saccharomyces / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D004272:DNA, Mitochondrial; D012331:RNA, Fungal; D003609:Dactinomycin