[Detection of anti-collagen type I and II antibodies by an immunoenzymatic technique (ELISA): results in rheumatoid arthritis, systemic lupus erythematosus and leprosy (author's transl)].
An attempt was made to detect antibodies against type I and/or II collagen in sera from patients with rheumatoid arthritis, systemic lupus erythematosus (SLE) and leprae. This study was performed with an immunoenzymatic technique: ELISA (enzyme-linked immunosorbent assay). The following steps were performed: bovine collagen type I or II was adsorbed on glass beads; possible free sites were saturated by incubating the beads with sheep serum; then, the antibodies specifically bound to collagen were detected by a peroxidase-labelled anti-immunoglobulin; the immune complexes at the surface of the beads were revealed by a substrate specific for peroxidase and of great stability: Trinder's reactive. Using conditions previously shown to be optimal, the prevalence of anti-collagen antibodies was as follows. In patients with lepromatous leprae the percentages of positive sera against collagen type I and II were 40% and 44%, respectively; in patients with tuberculoid leprae the percentages were lower: 10% and 30%, respectively. Ten per cent of the SLE patients had antibodies against collagen type I, half of the prevalence noted for anti-collagen type II antibodies (20%). Finally, 13.6% of the patients with rheumatoid arthritis had antibodies against collagen type I, a percentage very similar to that of the patients with anti-collagen type II antibodies (14.6%).
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D003094:Collagen / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D007918:Leprosy / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D003094:Collagen
Mise au point d'une technique immunoenzymatique (ELISA) pour la détection des anticorps anti-collagène de type I et II: recherche de ces anticorps au cours de la polyarthrite rhumatoïde, du lupus érythémateux disséminé et de la lèpre.