Pharmacology of narcotic analgesics in the horse: quantitative detection of morphine in equine blood and urine and logit-Log transformations of this data.
Morphine was detected in equine biological fluids by a combination of liquid-liquid extraction and column chromatography, followed by derivatization and gas-liquid chromatographic assay, using electron capture detector. Recovery of morphine from the equine biological samples was poor. However, despite an overall recovery of less than 20%, this method had a detection limit of 0.2 ng/ml. Addition of 5,000 U of bovine liver beta-glucuronidase/ml of urine enabled detection of the drug in urine for up to 144 hours after horses were given 0.1 mg of morphine/kg of body weight. Morphine was found for at least 24 hours in serum samples. An adaptation of logit-log transformation of gas-liquid chromatographic data for linearization over 3 log units suggested a simple adaptation to existing semiautomated data handling systems.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D005260:Female; D005966:Glucuronidase / Q000652:urine; D006736:Horses / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D009020:Morphine / Q000097:blood / Q000378:metabolism* / Q000652:urine; D011336:Probability; D013223:Statistics as Topic; D013997:Time Factors
D009020:Morphine; D005966:Glucuronidase