[Characterization of the functional state of the in vitro Hb-free perfused rat liver].
Rat liver was perfused in vitro with Krebs-Henseleit-medium and albumin at 25 degrees C in a recirculating system without hemoglobin over a period of 120 min. The following basic parameters for characterization of isolated liver perfusion were recorded: medium-pO2 prior and after liver passage, flow-rate, pH, and hepatic O2-consumption. Beyond this, concentration of lactate and pyruvate, hepatic glucose production, activity of aspartate-aminotransferase, leucine-aminopeptidase and acylase as well as concentration of K+-ions in the perfusion fluid were measured. In dependence on nutrition state of liver donors (fed or starved rats) the rate of glycogenolysis or rate of lactate-stimulated gluconeogenesis was calculated. The endogenous glycogenolysis can be blocked by an in vivo injection of propranolol. The propranolol-inhibited glycogenolysis can be stimulated by an in vitro glucagon application.
D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D005260:Female; D005943:Gluconeogenesis; D005947:Glucose / Q000096:biosynthesis; D006003:Glycogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption; D010477:Perfusion; D010952:Plasma Substitutes / Q000494:pharmacology; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D007773:Lactates; D010952:Plasma Substitutes; D011773:Pyruvates; D006003:Glycogen; D001219:Aspartate Aminotransferases; D007931:Leucyl Aminopeptidase; D000581:Amidohydrolases; C019672:aminoacylase I; D005947:Glucose
Charakterisierung des funktionellen Zustandes der in vitro Hb-frei perfundierten Rattenleber.