The effect of serum and plasma from haemodialysis patients on human mononuclear phagocytes cultured in vitro.
Human mononuclear phagocytes from healthy individuals were cultured in plasma and sera from normal persons and patients with chronic renal failure receiving haemodialysis. There was a marked detachment of macrophages from the glass coverslips when the cells were grown for 4 days in uraemic serum or plasma compared to normal serum. The ability of the macrophages to adhere to the glass coverslips was reduced when the cells were cultured in plasma from normal persons and uraemics subjected to systemic heparinization. However, the toxic effect of in vivo heparinization was much higher in the uraemics than in normal persons. Heparin added to normal and uraemic blood samples in vitro far in excess of in vivo heparinization did not influence the macrophage function to the same extent. Cell toxic substances seem to be produced as a consequence of systemic heparinization. These substances were not removed during 100 minutes of haemodialysis. Heparin added to pooled A Rh+ serum in concentrations far above those present in plasma following in vivo heparinization had little adverse effect on the macrophage function.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002448:Cell Adhesion; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007676:Kidney Failure, Chronic / Q000097:blood*; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D010587:Phagocytosis; D006435:Renal Dialysis; D014511:Uremia / Q000097:blood
D006493:Heparin