[Release of informosomes from isolated nuclei of wheat embryos in an in vitro system].
The properties of the informosomes released from isolated nuclei of wheat embryos were studied in an in vitro system. The release of the informosomes from isolated nuclei of wheat embryos labelled with [3H]uridine is stimulated by exogeneous ATP. Optimal conditions for informosomes release from nuclei were determined. The particles obtained have heterogeneous sedimentation coefficients in sucrose gradient ranging from 10 to 60S. The particles are sensitive to low concentrations of RNase. Buoyant density in CsCl is 1.40-1.43 g/cm3. RNA extracted from particles is characterized by heterogeneous distribution in sucrose gradient between 4 and 20S. The informosomes contain two main peptides with molecular mass 36 000 and 51 000 and a number of minor peptides.
D002458:Cell Fractionation; D002467:Cell Nucleus / Q000648:ultrastructure*; D002499:Centrifugation, Density Gradient; D007700:Kinetics; D012260:Ribonucleases; D012639:Seeds / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism
D012260:Ribonucleases
Osvovozhdenie informosom iz izolirovannykh iader zarodysheĭ. pshenitsy v sisteme in vitro.