On the dynamics of HLA-A2 antigen-antibody complexes on the cell membrane of human peripheral blood lymphocytes. A biochemical and electron microscopical autoradiographic study.
This report deals with some dynamic aspects of membrane bound immune complexes of HLA antigens and antibodies. Radiolabelled HLA antibodies were used to sensitize peripheral blood lymphocytes. Upon in vitro incubation of these cells, two different events were detected: 1) Immune complexes and a limited amount of free antibody were shed into the culture supernate; from the immune complexes specific antibodies could be regenerated. 2) Immune complexes were also internalized by means of endocytosis as visualized by electron microscopical autoradiography. Antibodies were found above dense bodies (lysosomes) and above multivesicular bodies. The results are discussed in relation to the biology of membrane bound HLA molecules.
D000936:Antigen-Antibody Complex / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron
D000936:Antigen-Antibody Complex; D006680:HLA Antigens