Modification of an in vitro technique in order to grow adult rat anterior pituitary in organ culture.
The study of adult rat anterior pituitary maintained in vitro was made possible by a modification of the suspension system used in the watch-glass method of organ culture. The integrity and health of the tissue was observed with the electron microscope. Somatotrophs and gonadotrophs were seen routinely. A cell with large (400-750 nm) granules and thought to be a luteotroph (mammotroph) was also seen. These results support the use of this method for the growth of adult rat anterior pituitary, or other large explants, for short periods of time in organ culture.
D000818:Animals; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D008297:Male; D009924:Organ Culture Techniques / Q000379:methods*; D010903:Pituitary Gland, Anterior / Q000166:cytology*; D051381:Rats; D011919:Rats, Inbred Strains