A micromethod for the induction and assay of specific in vitro antibody responses by human lymphocytes.
Human lymphocytes obtained from peripheral blood or spleen were cultured with influenza virus in a microculture system to produce strain specific antibody which was detected by the enzyme linked immunosorbent assay (ELISA). Nanogram amounts of specific antibody could be detected in cultures of 4 x 10(5) cells.
D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000914:Antibodies, Viral