Enzymatic formation of cholesteryl ester from cholesterol by gallbladder mucosa.
The formation of cholesteryl ester from cholesterol and acyl CoA catalyzed by the enzyme acyl CoA:cholesterol acyltransferase (EC 2.3.1.26) was studied in guinea pig gallbladder mucosa homogenate and the subcellular fractions. The enzymatic activity was enriched in the microsomal fraction. Highest activity was observed with palmitoyl, stearoyl or oleoyl CoA as substrate. Lowest activity was observed with linoleoyl CoA. These data elucidate one mechanism for the formation of cholesteryl ester from cholesterol by the gallbladder wall.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D001647:Bile Acids and Salts / Q000494:pharmacology; D002784:Cholesterol / Q000378:metabolism; D002788:Cholesterol Esters / Q000096:biosynthesis*; D005704:Gallbladder / Q000187:drug effects / Q000201:enzymology*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D009092:Mucous Membrane / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D002785:Sterol O-Acyltransferase / Q000378:metabolism*; D013379:Substrate Specificity
D001647:Bile Acids and Salts; D002788:Cholesterol Esters; D012710:Serum Albumin, Bovine; C014409:cholesteryl oleate; D002784:Cholesterol; D000217:Acyltransferases; D002785:Sterol O-Acyltransferase