[Effect of diet and microflora on amino sugars in the digestive contents and faeces of rats, lambs and preruminant calves].
Glucosamine (Glc NH2) and galactosamine (Gal NH2) were determined by an automatized chromatographic method in samples from digestive contents and faeces. Germfree lambs and rats as well as conventional preruminant calves, lambs and rats were used in the experiments. The rats were fed an irradiated casein diet, the lambs UHT milk and the preruminant calves various diets (table 1). Concentrations of Glc NH2 and of Gal NH2 reached 6 g/16 g of N in the caecal contents and the faeces of germfree rats (table 2). Concentrations of Gal NH2 were 7 to 12 times lower and those of Glc NH2 4 times lower in conventional rats. In lambs, the amino sugar concentrations (table 3) found in the stomach contents resembled those supplied by the milk. In contrast, in the large intestine and the faeces, the concentration of amino sugars was very high. In conventional lambs, those concentrations were 8 to 4 times lower. For ruminant calves, the concentrations of amino sugars (table 4) were greater in the ileal contents than in the faeces. They reached that of meconium when the calves were fed milk. The lowest values were observed in the faeces of calves fed soya, colostrum or fish diets which are less digestible than milk. In germfree animals, the hexosamines were endogenous. In conventional animals, bacterial metabolism led to an increase in faecal Glc NH2/Gal NH2. In the calves fed soya or fish diets, the hexosamine content in the faeces was low owing to the dilution of bacterial and endogenous mucoproteins by the undigested dietary proteins.
D000818:Animals; D002417:Cattle; D002432:Cecum / Q000378:metabolism; D004032:Diet; D004064:Digestive System / Q000378:metabolism*; D005243:Feces / Q000032:analysis*; D005688:Galactosamine / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism; D005856:Germ-Free Life; D005944:Glucosamine / Q000378:metabolism*; D007082:Ileum / Q000378:metabolism; D007420:Intestine, Large / Q000378:metabolism; D051381:Rats; D012756:Sheep
D005688:Galactosamine; D005944:Glucosamine
Influence de l'aliment et de la microflore sur la teneur en sucres aminés des contenus digestifs et des fèces chez le rat, l'agneau et le veau prèruminant.