Proteolytic digestion studies of chromatin core-histone structure. Identification of the limit peptides of histones H3 and H4.
Trypsin digestion of chromatin results in a well-defined set of limit peptides (P1--P5) derived from the four core histones. Those from histones H3 and H4 have been identified. P1 is H3 residues 27--129; P4 is H4 residues 18--102 and P5 is H4 residues 20--102. The N-terminal sequences removed correlate well with the regions that undergo post-synthetic acetylation and which show the greatest degree of sequence conservation. Autolytic digestion of chromatin releases a peptide (P1') from H3 representing residues 21--135. The implications of protease digestion for the higher order structure of chromatin are discussed.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D002843:Chromatin; D006657:Histones / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000302:isolation & purification*; D014357:Trypsin
D000596:Amino Acids; D002843:Chromatin; D006657:Histones; D010446:Peptide Fragments; D014357:Trypsin