Hemocyanin linked to protein A as an immunochemical labeling reagent for electron microscopy.
Hemocyanin-protein A (Hcy/A) is an immunochemical marker suitable for both transmission and scanning electron microscopy. A method is described for the preparation of highly monomeric Hcy/A where protein A and hemocyanin are present in the conjugate in a nearly equal molar ratio. Examples of Hcy/A labeling of several cellular antigens are provided.
D000818:Animals; D000941:Antigens / Q000032:analysis; D002645:Chickens; D004912:Erythrocytes / Q000276:immunology; D006433:Hemocyanins; D006801:Humans; D007120:Immunochemistry; D007202:Indicators and Reagents; D051379:Mice; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013205:Staphylococcal Protein A
D000941:Antigens; D007202:Indicators and Reagents; D013205:Staphylococcal Protein A; D006433:Hemocyanins