Enzyme-linked immunosorbent assay (ELISA) for detection of antibodies against glycosphingolipids and its application to patients with systemic lupus erythematosus.
Enzyme-linked immunosorbent assay (ELISA) to determine the binding of antibodies to glycosphingolipid antigens is described. This technique is rapid, specific, sensitive and convenient. The technique is applicable to detect anti-glycolipid antibodies in sera of patients with some autoimmune diseases.
D000906:Antibodies / Q000032:analysis*; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006028:Glycosphingolipids / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*
D000906:Antibodies; D006028:Glycosphingolipids; D007074:Immunoglobulin G; D007075:Immunoglobulin M