title: Use of solid medium for isolation of leptospires of the Hebdomadis serogroup from bovine milk and urine.
abstract: Leptospira interrogans serovars hardjo and szwajizak were isolated from the milk of experimentally infected cows only when using bovine albumin polysorbate-80 solid medium. Semisolid media, with and without the addition of 5-fluorouracil, and hamster inoculations were also used in the isolation attempts. These cultural isolation methods were also compared using cows' urine. Semisolid medium with 5-fluorouracil proved to be the most successful. However, the other methods were satisfactory, with solid medium having the lowest percentage of cultural isolations.
mesh_terms: D000818:Animals; D002417:Cattle / Q000382:microbiology*; D006224:Cricetinae; D003470:Culture Media; D005260:Female; D005472:Fluorouracil; D007921:Leptospira interrogans / Q000254:growth & development / Q000302:isolation & purification*; D008647:Mesocricetus / Q000382:microbiology; D008892:Milk / Q000382:microbiology*; D011136:Polysorbates; D014556:Urine / Q000382:microbiology*
chemical_list: D003470:Culture Media; D011136:Polysorbates; D005472:Fluorouracil