The incorporation in vitro of sulphate ions into synaptic-membrane glycoproteins.
Synaptosomes from sheep brain cortex were incubated with carrier-free Na235SO4 and the synaptic plasma membranes were isolated. The membranes were free of contamination from cytosol, mitochondria and microsomal material and accounted for 30% of the radioactivity present in the synaptosomal particulate fraction. Control experiments demonstrated that the radioactivity present in the preparation was not due to non-specific binding of sulphate ions. The synaptic membranes contained at least six 35S-containing protein bands with molecular weights between 160 000 and 16 000. Analysis showed that the radioactivity was located in the carbohydrate moiety of a glycopeptide.
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000378:metabolism*; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000378:metabolism*; D012756:Sheep; D013347:Subcellular Fractions / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism*; D013570:Synaptic Membranes / Q000378:metabolism*
D006023:Glycoproteins; D008565:Membrane Proteins; D013431:Sulfates