Biochemical and biophysical behaviors of plasma albumin. II. Comparison of plasma albumin from normal subjects and nephrotic patients.
Biochemical and biophysical analyses were made to study the properties of serum albumin from nephrotic patients of two types, remissive stage and relapsed state, and the results were compared with those in normal subjects. Two different ion exchange columns were prepared to isolate and purify each albumin sample and the materials eluted through the two columns were identified. The nephrotic elution materials, especially in the state of relapse, were of some difference from those of normal subjects. alpha-Helix contents estimated by circular dichroism spectra were found to be closer to normal albumin in remissive state than in the state of relapse. The binding of fluorescent probe, 1-anilino-8-naphthalene sulfonate, to albumin was examined by calculating the binding parameter, the dissociation constant and number of binding sites. The binding parameters were closer to those of normal albumin in remissive stage than the state of relapse.
D000293:Adolescent; D000817:Anilino Naphthalenesulfonates / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D002942:Circular Dichroism; D005260:Female; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D008297:Male; D009401:Nephrosis / Q000097:blood*; D011485:Protein Binding; D012709:Serum Albumin / Q000032:analysis*
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D012709:Serum Albumin; C027132:1-anilino-8-naphthalenesulfonate