title: Activity of some enzymes involved in "adenylate cycle" in rat embryo cells infected with parvoviruses (X14, H-1).
abstract: In rat embryo cell cultures infected with X14 or H-1 parvovirus and in mock-infected cell cultures the activity of some enzymes involved in purine nucleotide interconversion and in "adenylate cycle" was determined. The enzymatic activities have been assayed on 100,000 x g supernatant by spectrophotometric methods, measuring the absorbance variation in U.V. and by radiometric methods, resolving the radioactive products of reaction by TLC on PEI cellulose. The results indicated a decrease of the enzymatic activities that degrade purine nucleosides and nucleotides in infected cells compared to the controls. Some different behaviour patterns showed the enzymes involved in base salvage pathway; adenine phosphoribosyltransferase did not show a significant modification of activity, whereas hypoxanthine (guanine) phosphoribosyltransferase increased slightly in X14 virus-infected cells. The behaviour of the above mentioned enzymatic activities may be considered as a mechanism of purine nucleotide saving, coupled to an active salvage pathway for the synthesis of nucleotides required for the viral replication.
mesh_terms: D000225:Adenine / Q000378:metabolism*; D000619:Aminohydrolases / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000201:enzymology*; D005260:Female; D010321:Parvoviridae; D010430:Pentosyltransferases / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D013056:Spectrophotometry, Ultraviolet; D014777:Virus Diseases / Q000201:enzymology*
chemical_list: D010430:Pentosyltransferases; D000619:Aminohydrolases; D000225:Adenine