Carbon monoxide- and oxygen-reacting haemoproteins in the mitochondrial fraction from the soil amoeba Acanthamoeba castellanii. Studies at subzero temperatures.
1. Mitochondria-enriched fractions of the soil amoeba Acanthamoeba castellanii contained four haemoproteins that in their reduced forms reacted with CO to give photodissociable CO complexes; these were cytochromes a 3, a 614, b- and c-type cytochromes. 2. Non-photodissociable oxygen-containing compounds were formed at temperatures between -130 and -150 degrees C after photodissociation of CO in the presence of 200 microM-O2, 3. Electron transport, indicated by the oxidation of cytochromes a + a3 and cytochrome c, did not occur until the temperature was raised to -80 degrees C.
D000656:Amoeba / Q000378:metabolism*; D002248:Carbon Monoxide / Q000378:metabolism*; D003080:Cold Temperature; D003580:Cytochromes / Q000378:metabolism*; D004579:Electron Transport; D008928:Mitochondria / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010782:Photolysis; D013053:Spectrophotometry; D013367:Submitochondrial Particles / Q000378:metabolism
D003580:Cytochromes; D002248:Carbon Monoxide; D010100:Oxygen