A hybridoma monoclonal antibody which reacts with cells carrying the HLA-A2 antigen: evidence for heterogeneity in the expression of HLA-A2.
A monoclonal antibody, FMC 5, has been prepared against human lymphocytes and its reactivity examined. FMC 5 behaves, for the most part, as an anti-HLA-A2 typing serum. However, three small groups of discrepant types were identified, and each provides additional insight into the complexity of the MHC antigens. A small number (5/60) of donors typed as HLA-A2 did not react with FMC 5. A further group (6/35) were HLA-A2 and did react with FMC 5, but lysis was always incomplete in this group. Finally, 13/39 non-A2 donors gave various degrees of reactivity with FMC 5. Lysis in this group was usually incomplete and required a high antibody concentration.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D006680:HLA Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D011110:Polymorphism, Genetic
D000911:Antibodies, Monoclonal; D006680:HLA Antigens