title: Membrane proteins in human skin tumors.
abstract: The microsomal, mitochondrial, and nuclear fractions of cutaneous tumors have been investigated. Cutaneous tumors were homogenized and microsomal, mitochondrial, and nuclear fractions were purified. The membrane proteins were solubilized with sodium duodecyl sulfate (SDS). The membrane lipids were removed and membrane proteins were solubilized again in a small volume of SDS solution and chromatographed in SDS-acrylamide slab-gel. The plates were stained with Coomassie Brilliant Blue to show protein bands. The preliminary results show that the electrophoretic profiles of microsomal proteins are characteristic of some tumors.
mesh_terms: D002277:Carcinoma / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000032:analysis; D002850:Chromatography, Gel; D005350:Fibroma / Q000032:analysis; D006801:Humans; D007425:Intracellular Membranes / Q000032:analysis; D008545:Melanoma / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis*; D008861:Microsomes / Q000032:analysis; D008928:Mitochondria / Q000032:analysis; D009363:Neoplasm Proteins / Q000032:analysis*; D009685:Nuclear Envelope / Q000032:analysis; D012878:Skin Neoplasms / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate
chemical_list: D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012967:Sodium Dodecyl Sulfate