Relations between iron and vanadium metabolism: in vivo incorporation of vanadium into iron proteins of the rat.
In vivo experiments with 48V and 59Fe radiotracers were performed to study the association of V with Fe proteins. Each male rat was injected ip with 10 micrograms 48VO2+ and then with 1 microgram 59Fe3+ to label Fe-containing proteins. The radioactivities incorporated were measured in plasma transferrin, red blood cell hemoglobin, liver ferritin, partially purified heart myoglobin, and liver mitochondrial and microsomal cytochromes b and c and ferriporphyrin. Liver ferritin can bind V in vivo similarly to plasma transferrin, as shown by gel filtration and immunoprecipitation. Negligible amounts of 48 V were incorporated into hemoglobin, partially purified myoglobin, and cytochromes b and c. These findings suggest that the nonenzymatic Fe-containing proteins may be involved in the V metabolism.
D000818:Animals; D003600:Cytosol / Q000378:metabolism; D005293:Ferritins / Q000378:metabolism; D006420:Hemeproteins / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D011485:Protein Binding; D051381:Rats; D011919:Rats, Inbred Strains; D014639:Vanadium / Q000378:metabolism*
D006420:Hemeproteins; D014639:Vanadium; D005293:Ferritins; D007501:Iron