[Composition of the histone-nonhistone protein complex isolated from calf thymus chromatin].
The relative content of protein and total histone of chromatin complex was compared after gel-chromatographical separation with subsequent electrophoresis of the obtained fractions in polyacrylamide gel. The H1 and H4 histones relative amounts did not differ from the calculated values while in the complex the H1 content was lower and that of H4 was almost twice as high. Three histone fractions of the complex (H2a, H2b and H4) contained nonhistone proteins which were not separated by gel chromatography. The H2a histone fraction had the most rich spectrum of nonhistone proteins (6 lines). Three high-molecular nonhistone protein fractions out of ten revealed in the complex could be found only in the alkaline medium in the presence of sodium dodecyl sulphate.
D000818:Animals; D002417:Cattle; D002843:Chromatin / Q000032:analysis*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D006657:Histones / Q000032:analysis*; D046911:Macromolecular Substances; D013950:Thymus Gland / Q000032:analysis
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D046911:Macromolecular Substances
Sostav kompleksa gistony - negistonovye belki, bydelennogo iz khromatina timusa telenka.