Estrogen receptor measurement in low-protein breast cancer cytosols. A modified charcoal technique.
The assay of estrogen receptor (ER) in human breast cancer biopsies is usually excellent using the dextrancoated charcoal method. If the cytosol protein concentration is below 1 mg/ml, however, underestimation of ER content occurs presumably due to adsorption of ER to the charcoal. This problem can be minimized if 1 mg/ml of bovine serum albumin (BSA) is added to the charcoal solution prior to use.
D001706:Biopsy; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000032:analysis*; D002606:Charcoal; D003600:Cytosol / Q000032:analysis*; D006801:Humans; D008722:Methods; D009363:Neoplasm Proteins / Q000032:analysis*; D011960:Receptors, Estrogen / Q000032:analysis*; D012710:Serum Albumin, Bovine
D009363:Neoplasm Proteins; D011960:Receptors, Estrogen; D002606:Charcoal; D012710:Serum Albumin, Bovine