Pentagastrin stimulation of DNA synthesis and thymidine kinase activity in gastric mucosa of rats.
Following four equally spaced injections (at 12-hour intervals) of pentagastrin (250 microgram/kg) to fasted rats the incorporation of [3H]-thymidine into gastric mucosal DNA in vivo and in vitro was significantly increased by 30 and 58%, respectively, when compared with the corresponding saline control. Pentagastrin treatments also caused a significant 50% enhancement in gastric mucosal thymidine kinase activity. It is suggested that the hormone-mediated stimulation in thymidine kinase may, in part be due to the presence of a physiological activator for the enzyme in the tissue.
D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D010418:Pentagastrin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013936:Thymidine / Q000378:metabolism; D013937:Thymidine Kinase / Q000378:metabolism*
D004247:DNA; D013937:Thymidine Kinase; D010418:Pentagastrin; D013936:Thymidine