Effects of hypocholesterolemia and chronic hormonal stimulation on sterol and steroid metabolism in a Leydig cell tumor.
The studies presented herein were done to investigate the effects of drug-induced hypocholesterolemia and chronic hormonal stimulation on cholesterol metabolism and steroid biosynthesis in a functional Leydig cell tumor. It was found that 4-aminopyrazolo(3,4-d)-pyrimidine (4-APP)-induced hypocholesterolemia had no effect on a) the amount of cholesterol present in the tumor, b) cholesterol biosynthesis, and c) steroid production. Chronic stimulation with choriogonadotropin also had no effect on the amount of cholesterol present in the tumor, but it increased steroid production and cholesterol biosynthesis. These results suggest that the Leydig tumor cells primarily use intracellular cholesterol for steroid biosynthesis. Other data show that 4-APP treatment reduces gonadotropin binding in the Leydig tumor cells.
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D002784:Cholesterol / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D050171:Dyslipidemias / Q000150:complications / Q000378:metabolism*; D007700:Kinetics; D007984:Leydig Cell Tumor / Q000150:complications / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000150:complications / Q000378:metabolism; D013256:Steroids / Q000378:metabolism*; D013261:Sterols / Q000378:metabolism*; D013736:Testicular Neoplasms / Q000150:complications / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D013256:Steroids; D013261:Sterols; C004420:4-aminopyrazolo(3,4-d)pyrimidine; D002784:Cholesterol; D000225:Adenine