A rapid new method of measuring phagocytosis and cytotoxicity in macrophage tissue cultures.
111Indium as a radioactive tracer for labelling cells has advantages of 51 chromium: very low leakage after 24 h, superior counting properties of the radionuclide 111indium, and a labelling yield of greater than 70%. Tissue culture chamber slides combine the advantage of tissue culture micromethods with simple preparation techniques used in histology. We report here results with peritoneal macrophages as effector cells and erythrocytes and tumor cells as targets. Phagocytosis and cytotoxicity were measured easily and rapidly.
D000327:Adsorption; D000818:Animals; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007942:Leukemia, Experimental / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D009439:Neuraminidase / Q000494:pharmacology; D010587:Phagocytosis; D051381:Rats; D011919:Rats, Inbred Strains; D012513:Sarcoma, Experimental / Q000276:immunology
D009439:Neuraminidase