title: Analysis of 99mTc-pyridoxylidene-glutamate complex formation and its preparation with tin-adsorbed-resin: Sn-Resin kit method.
abstract: 99mTc-Pyridoxylidene-glutamate (Tc-PG) was introduced in 1975 by Baker et al. as a cholescintigraphic agent. Nevertheless its routine use has been limited due to the autoclaving process involved. To shorten the labeling procedure, an analysis of Tc-PG complex formation using the stannous chloride method was carried out. Sn-Resin (stannous ion adsorbed onto cation exchange resin) was used for labeling a stable Tc-PG with high efficiency. A preautoclaved complex of pyridoxal and glutamate was required but the labeling procedure took only 10-15 min after the elution of 99m-TcO4- from the generator. Formation of a complex other than a 99mTc-complex of the Schiff -base ligand pyridoxylidene-glutamate is discussed. The new formulation of a Tc-PG kit (Sn-Resin) simplified the labeling method and reproducible data to that already reported by Baker was obtained in chromatographic studies. Its suitability in hospital departments has already been shown in clinical studies.
mesh_terms: D001646:Bile / Q000000981:diagnostic imaging; D002411:Cation Exchange Resins; D004355:Drug Stability; D005971:Glutamates; D006801:Humans; D007475:Ion Exchange Resins; D007553:Isotope Labeling; D015609:Organotechnetium Compounds; D011730:Pyridoxal / Q000031:analogs & derivatives*; D011877:Radionuclide Imaging; D011933:Reagent Kits, Diagnostic; D013667:Technetium; D014001:Tin; D017971:Tin Compounds
chemical_list: D002411:Cation Exchange Resins; D005971:Glutamates; D007475:Ion Exchange Resins; D015609:Organotechnetium Compounds; D011933:Reagent Kits, Diagnostic; D017971:Tin Compounds; C011551:technetium Tc 99m pyridoxylidene glutamate; C023599:stannous chloride; D011730:Pyridoxal; D013667:Technetium; D014001:Tin