Potentials and limitations of a clonogenic cell assay for human brain tumors.
Single cells from biopsies of ten malignant gliomas were treated with 1,3-bis (2-chloroethyl)-1-nitrosourea (BCNU) in vitro, and tumor cell survival was compared to patient response to nitrosourea therapy. The limitations of the methods and their interpretations and future prospects for clonogenic cell analyses are discussed.
D000328:Adult; D000368:Aged; D001932:Brain Neoplasms / Q000000981:diagnostic imaging / Q000188:drug therapy*; D002330:Carmustine / Q000627:therapeutic use*; D002478:Cells, Cultured; D002999:Clone Cells; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005260:Female; D005910:Glioma / Q000000981:diagnostic imaging / Q000188:drug therapy*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014057:Tomography, X-Ray Computed
D002330:Carmustine