title: Effects of various gastrointestinal peptides on gastric somatostatin release.
abstract: Effects of various gastrointestinal peptides on gastric somatostatin release from the isolated perfused rat stomach were studied. After isolation of the stomach in a fasted rat by the method of Lefébvre and preperfusion with 4.6% dextran-Krebs-Ringer bicarbonate buffer containing 5.5 mM glucose, each peptide was infused into the left gastric artery at a constant rate for 15 min. Secretin and bombesin caused a significant increase of gastric somatostatin release in a dose-related manner (10(-8)-10(-6) M). Gastric somatostatin release was also stimulated after the administration of pentagastrin (10(-8)-10(-6) M). In contrast, both methionine-enkephalin and substance P decreased gastric somatostatin release in a dose-related manner (10(-8)-10(-6) M), whereas neurotensin (10(-8)-10(-6) M) failed to change it significantly. The present results suggest that these various gastrointestinal peptides may regulate gastric somatostatin secretion.
mesh_terms: D000818:Animals; D001839:Bombesin / Q000494:pharmacology; D004745:Enkephalins / Q000494:pharmacology; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D005768:Gastrointestinal Hormones / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009496:Neurotensin / Q000494:pharmacology; D010418:Pentagastrin / Q000494:pharmacology; D010477:Perfusion; D051381:Rats; D012633:Secretin / Q000494:pharmacology; D013004:Somatostatin / Q000378:metabolism*; D013373:Substance P / Q000494:pharmacology
chemical_list: D004745:Enkephalins; D005768:Gastrointestinal Hormones; D012633:Secretin; D013373:Substance P; D009496:Neurotensin; D013004:Somatostatin; D010418:Pentagastrin; D001839:Bombesin