title: Identification of fatty acid synthetase messenger RNA on free polyribosomes isolated from lactating rabbit mammary gland.
abstract: The synthesis of fatty acid synthetase on free polyribosomes from lactating rabbit mammary gland was demonstrated by using polyribosomes run-off techniques and immunochemical identification of products with synthetase antiserum. Several reproducible and discrete immunoprecipitable polypeptides were observed which were within the molecular-weight range of the synthetase subunit (235 000--252 000), as well as several of lower molecular weight.
mesh_terms: D000818:Animals; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D064429:Fatty Acid Synthases / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000032:analysis; D011132:Polyribosomes / Q000378:metabolism*; D011247:Pregnancy; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits
chemical_list: D010455:Peptides; D012333:RNA, Messenger; D064429:Fatty Acid Synthases