Developmental changes in glucose transport of guinea pig erythrocytes.
The developmental changes in the capacity for D-glucose transport of guinea pig erythrocyte membranes were compared to alterations in the electrophoretic pattern of erythrocyte membrane components. Guinea pig erythrocytes lose their D-glucose carrier functions during development. Good correlation was observed between the loss of glucose uptake and apparent decrease of the zone 4.5 of Coomassie Blue-stained membrane proteins on electrophoresis. Reconstitution of membrane preparations in liposomes resulted in a parallel change in the D-glucose uptake and D-glucose penetration of intact erythrocytes. This suggests that the decrease of D-glucose transport capacity during development is caused by the loss of one or more protein components from the erythrocyte membranes.
D000367:Age Factors; D000818:Animals; D001693:Biological Transport, Active; D001786:Blood Glucose / Q000378:metabolism*; D002352:Carrier Proteins / Q000097:blood / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006168:Guinea Pigs; D008081:Liposomes; D008565:Membrane Proteins / Q000097:blood
D001786:Blood Glucose; D002352:Carrier Proteins; D008081:Liposomes; D008565:Membrane Proteins