Identification of mycobacterium chelonei by thin-layer chromatographic analysis of whole-organism methanolysates.
Two-dimensional thin-layer chromatography of whole-organism acid methanolysates of Mycobacterium chelonei gives a characteristic pattern of two non-polar mycolic acid methyl esters. All other mycobacteria examined so far contain mycolic acids with polar oxygen functions in addition to the 3-hydroxy acid unit.
D002855:Chromatography, Thin Layer; D004952:Esters; D009161:Mycobacterium / Q000032:analysis*; D009171:Mycolic Acids / Q000032:analysis*
D004952:Esters; D009171:Mycolic Acids