Induction of acyl-CoA dehydrogenases and electron transfer flavoprotein and their roles in fatty acid oxidation in rat liver mitochondria.
The capacity of mitochondrial beta-oxidation and activities of three acyl-CoA dehydrogenases and electron transfer flavoprotein in rat liver were increased after administration of peroxisome proliferators such as di(2-ethylhexyl)phthalate or p-chlorophenoxyisobutyrate. Oxygen consumption by mitochondrial beta-oxidation with palmitate, palmitoyl-CoA or palmitoylcarnitine was in the range of 0.27-0.41 mumol O2/min per g liver and the dehydrogenase activity with palmitoyl-CoA was 0.56 mumol/min per g liver. These activities were increased, after administration of peroxisome proliferators, to 0.80-1.23 mumol O2/min per g liver and 1.06-1.08 mumol/min per g liver, respectively. The time required to reach half-maximal induction of the three acyl-CoA dehydrogenases and electron transfer flavoprotein after administration of di(2-ethylhexyl)phthalate was about 5 days. On the withdrawal of the agent, the activities of these flavoproteins decreased to the control levels with half-lives of about a day.
D000818:Animals; D002994:Clofibrate / Q000031:analogs & derivatives*; D002995:Clofibric Acid / Q000494:pharmacology*; D004051:Diethylhexyl Phthalate / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D044943:Fatty Acid Desaturases / Q000096:biosynthesis*; D005227:Fatty Acids / Q000378:metabolism*; D005420:Flavoproteins / Q000096:biosynthesis*; D008830:Microbodies / Q000187:drug effects; D008930:Mitochondria, Liver / Q000201:enzymology*; D010795:Phthalic Acids / Q000494:pharmacology*; D051381:Rats
D005227:Fatty Acids; D005420:Flavoproteins; D010795:Phthalic Acids; D002995:Clofibric Acid; D004051:Diethylhexyl Phthalate; D044943:Fatty Acid Desaturases; D002994:Clofibrate