title: Improvements in the synthesis of secretin by the repetitive excess mixed anhydride (REMA) method.
abstract: Protected secretin, a 27-peptide amide, was synthesized by the all-repetitive excess mixed anhydride (REMA) method and purified by preparative reverse-phase high-performance liquid chromatography. Highly potent secretin was obtained after deprotection with the aid of HF/anisole and purification by ion-exchange chromatography. The scope of the REMA-strategy is discussed in comparison with other strategies.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000812:Anhydrides; D000818:Animals; D002415:Cats; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D008722:Methods; D012633:Secretin / Q000138:chemical synthesis* / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D000812:Anhydrides; D012633:Secretin