title: Biosynthetic studies on the collagenous components of basement membranes.
abstract: The biosynthesis of the collagenous components of the rat lens capsule and glomerular basement membrane (GBM) has been investigated. Intact lenses, isolated lens capsules and isolated renal glomeruli incorporate [3H]proline into 4-hydroxy[3H]proline containing polypeptides of approximate molecular weight 180,000 (analysed under reducing conditions). These polypeptides are deposited in the deoxycholate-insoluble basement membrane matrix and correspond to subunits of the intact, non-radioactive basement membranes. With time a proportion of the newly synthesised collagenous polypeptides become assembled into high molecular weight aggregates which are stabilized by lysine- and/or hydroxylysine-derived cross-links. Despite the similar patterns of collagen biosynthesis in the rat lens capsule and GMB, the latter has a more complex polypeptide composition the origin of which remains uncertain.
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000378:metabolism*; D002850:Chromatography, Gel; D003094:Collagen / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D006909:Hydroxyproline / Q000096:biosynthesis; D066298:In Vitro Techniques; D007678:Kidney Glomerulus / Q000378:metabolism*; D007908:Lens, Crystalline / Q000378:metabolism*; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification; D011392:Proline / Q000494:pharmacology; D051381:Rats
chemical_list: D010455:Peptides; D003094:Collagen; D011392:Proline; D006909:Hydroxyproline