Mammalian culture systems for the study of genetic effects of N-substituted aryl compounds.
Liver-derived intact cell systems are useful for examination of the genetic effects of N-substituted aryl compounds. With freshly isolated hepatocytes, the hepatocyte primary culture-DNA repair test detects the genotoxicity of a spectrum of activation-dependent aryl amines and amides. Likewise, continuous lines of adult rat liver epithelial cells are mutated at the hypoxanthine-guanine phosphoribosyl locus by such compounds. Hepatocyte-mediated mutagenesis enhances the activation of aromatic amines to mutagenic metabolites. These intact cell systems provide a balance of detoxification and activation reactions for evaluation of tissue profiles of metabolism and capability for the production of genotoxic metabolites.
D000588:Amines / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects*; D006801:Humans; D009152:Mutagenicity Tests; D009153:Mutagens; D009154:Mutation; D013329:Structure-Activity Relationship
D000588:Amines; D009153:Mutagens