Glucose-induced release of immunoreactive gastric inhibitory polypeptide (IR-GIP) into the duodenal lumen in man.
Duodenal juice was collected before and after an intragastric infusion of 300 ml 25% glucose in five healthy subjects. After extraction with 95% ethanol, IR-GIP in duodenal juice was determined by radioimmunoassay and found to increase from mean basal level of 63 pmol/l to a mean peak of 354 pmol/l after the glucose infusion. The elution diagrams of IR-GIP in glucose-stimulated duodenal juice and the corresponding plasma from a 16 X 400 mm Sephadex G-50 Fine column were similar, and the radioimmunoassay dilution curves of porcine GIP and duodenal juice IR-GIP were superimposable.
D000328:Adult; D000818:Animals; D000941:Antigens; D002850:Chromatography, Gel / Q000379:methods; D004386:Duodenum / Q000378:metabolism*; D005260:Female; D005749:Gastric Inhibitory Polypeptide / Q000032:analysis / Q000378:metabolism*; D005768:Gastrointestinal Hormones / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007419:Intestinal Secretions / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay / Q000379:methods; D013268:Stimulation, Chemical; D013552:Swine
D000941:Antigens; D005768:Gastrointestinal Hormones; D005749:Gastric Inhibitory Polypeptide; D005947:Glucose