title: Influence of temperature on the growth in cell culture of malignant catarrhal fever virus.
abstract: The growth characteristics of malignant catarrhal fever (MCF) virus in bovine thyroid cultures were affected by incubation temperature. The cytopathic effect at 37 degrees C was predominantly syncytial and little or no cell-free virus could be detected. At 32 degrees to 34 degrees C foci of rounded refractile cells were observed, and this was accompanied by an increase in the amount of cell-free virus found in culture fluids. Growth curve studies with one low passage isolate of MCF virus showed that optimum yields of cell-free virus were obtained at 32 degrees to 34 degrees C and survival curves at 32 degrees and 37 degrees C indicated that this was a result of the relatively short half life of the virus at the higher temperature. A number of other benefits resulted from the use of lowered incubation temperature and these are discussed with reference to in vitro work with the virus.
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D006564:Herpesviridae / Q000254:growth & development* / Q000302:isolation & purification; D008304:Malignant Catarrh / Q000382:microbiology*; D013696:Temperature