Buffalo alpha S1-casein; its terminal residues and amino acid composition.
Buffalo alpha S1-casein was treated with carboxypeptidase-A to determine the C-terminal amino acid residue, which was - leucine-tryptophan. The Sakaguchi reagent was used to confirm arginine as the N-terminal amino acid. The colored p-phenylazophenyl thiohydantoins of tryptophan and arginine released from C- and N-terminals respectively were quantitatively estimated and from this molecular weights of buffalo alpha S1 casein were calculated and found to be 26,300 and 26,100 respectively.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002020:Buffaloes; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D002364:Caseins; D002417:Cattle; D013045:Species Specificity
D000596:Amino Acids; D002364:Caseins; D002268:Carboxypeptidases; D043422:Carboxypeptidases A