[Obtaining purified erythropoietin from rabbit plasma].
A scheme of preparing partly purified erythropoietin from rabbit blood plasma is offered. The factor of purification is about 25000. Erythropoietin was purified by ion exchange chromatography on DE-32 cellulose, affinity chromatography on phytohemagglutinin-P and by gel filtration through Ultragel ACA-44. The specific activity of erythropoietin thus obtained was about 75-100 units per mg protein. The preparation may be used for exploring the regulation and differentiation of red blood cells and as an initial material for obtaining high-purified erythropoietin..
D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004921:Erythropoietin / Q000302:isolation & purification*; D008722:Methods; D011817:Rabbits
D004921:Erythropoietin
Poluchenie ochishchennogo éritropoetina iz plazmy krovi krolika.