[Immunopharmacological actions of neurotropin. (1). Immunostimulating actions of neurotropin (author's transl)].
Neurotropin (NSP) is an extract isolated from vaccinia virus-inoculated and inflamed skin of rabbits. The present study was undertaken to examine the immunostimulating actions of NSP. NSP alone did not induce a proliferation of spleen cells from mice or human peripheral blood lymphocytes (PBL). However, NSP clearly enhanced concanavalin A (Con A)-induced proliferation of both spleen cells and human PBL, but slightly inhibited lipopolysaccharide (LPS)-induced proliferation of spleen cells. NSP reversed the decreases of Con A-induced proliferation of spleen cells pretreated with mitomycin C (MMC) in a dose of 5 microgram/ml or irradiated at 100 to 500 R, while NSP did not reverse the decreases of LPS-induced proliferation of spleen cells pretreated with MMC, in a dose of 5 to 50 microgram/ml, or irradiated at 100 to 1000 R. NSP enhanced in a dose-dependent fashion one-way mixed lymphocytes culture (MLC) reaction using MMC-treated spleen cells from BDF1 mice as stimulator cells and those from C57BL/6 mice as responder cells, respectively. NSP reversed the considerable decrease of MLC-mediated proliferation of responder cells pretreated with MMC, in a dose of 5 to 10 microgram/ml, or irradiated at 100 to 250 R. These results indicate that NSP activates T cell function participating in cell-mediated immunity.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D004357:Drug Synergism; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008937:Mitomycins / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D011509:Proteoglycans / Q000494:pharmacology; D013154:Spleen / Q000166:cytology
D000276:Adjuvants, Immunologic; D008070:Lipopolysaccharides; D008937:Mitomycins; D011134:Polysaccharides; D011509:Proteoglycans; D003208:Concanavalin A; C010770:polysaccharide-K; C010491:neurotropin