Specificity of two anti-human albumin monoclonal antibodies.
We have studied the reaction of two monoclonal anti-human albumin antibodies with various fragments of albumin comprising the entire molecule. Using solid phase assay, inhibition of enzyme immunoassay and of passive hemagglutination, they were shown to be specific for a fragment F1, of 6000 dalton, located near the C terminus of human albumin. In addition, these antibodies reacted weakly with fragment D which corresponds to the N terminal half of the molecule.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000941:Antigens; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D010446:Peptide Fragments / Q000276:immunology; D012709:Serum Albumin / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D010446:Peptide Fragments; D012709:Serum Albumin