Enrichment of reticulocytes by lectin-mediated agglutination.
Bloods of both normal and anemic (bleeding anemia) rabbits as well as of a healthy man (blood group A1) have been treated with varying concentrations of the lectin anti-AHP. Under defined conditions, mature erythrocytes are agglutinated selectively whereas reticulocytes remain suspended as single cells in the supernatant fluid. With this procedure an enrichment of reticulocytes of about tenfold with a maximum recovery of nearly 100% was achieved.
D000017:ABO Blood-Group System; D000740:Anemia / Q000097:blood; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000276:immunology; D006372:Helix, Snails / Q000276:immunology; D006384:Hemagglutination; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D011817:Rabbits; D012156:Reticulocytes / Q000276:immunology
D000017:ABO Blood-Group System; D037102:Lectins