[ATP, ADP and AMP content in the low-temperature preservation of lymphoid cells].
As compared with ATP and ADP concentration in native lymphocytes, content of ATP was decreased down to 26.3% and 35.1% and of ADP--37.0% and 44.4% in the cell preparations after conservation in mixtures containing 10% PEO-400 and dimethyl-sulfoxide (DMSO). AMP was not found in the lymphocytes when both PEO-400 and DMSO were used. Mechanisms responsible for instability of lymphocyte adenine nucleotides under conditions of low temperature conservation are discussed.
D000227:Adenine Nucleotides / Q000032:analysis*; D000244:Adenosine Diphosphate / Q000032:analysis; D000249:Adenosine Monophosphate / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D001793:Blood Preservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005609:Free Radicals; D005615:Freezing; D008214:Lymphocytes / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D008297:Male; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D013997:Time Factors
D000227:Adenine Nucleotides; D003451:Cryoprotective Agents; D005609:Free Radicals; D011092:Polyethylene Glycols; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D004121:Dimethyl Sulfoxide
Soderzhanie ATF, ADF i AMF pri nizkotemperaturnom konservirovanii limfoidnykh kletok.