Histochemical and histoenzymatic action of crinofizin in cystic mastosis and prostate adenoma studied in cell and organotypic cultures.
The work deals with the study at cell level of the action of Crinofizin on some target organs. The effect of various doses of Crinofizin (0.2, 0.1, 0.01 and 0.001 ml/culture tube) was tested on multiplication and protein synthesis in human mammary tissue cells (cystic mastosis) and human prostate adenoma in cell culture. The morphologic aspect, enzymatic activity (LDH, G-6-PDH) and mucopolysaccharides synthesis were also studied. It was found that administration of Crinofizin results in preferential development of epithelial cells at the expense of the connective-fibroblastic ones. LDH and G-6-PDH, higher in the control cultures of the epithelial type than in the fibroblastic ones, becomes uniform by decreasing in intensity in the treated lots. Acute experimentation carried out on prostate organ cultures from rats of various ages (30, 60, 90 days and 2 years) treated with 0.2ml Crinofizin/ml culture medium indicates a decrease in connective tissue intrafollicular stroma, which is well developed in the case of adenoma. LDH and G-6-PDH activity, which is higher in the case of adenoma, decreases under Crinofizin administration to the levels in the adult control. The histologic aspect of the hypertrophied prostate organ cultures from aged rats, becomes after Crinofizin administration similar to that of the adult rat organ cultures.
D000236:Adenoma / Q000201:enzymology / Q000473:pathology*; D001941:Breast Diseases / Q000201:enzymology / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D009924:Organ Culture Techniques; D010455:Peptides / Q000494:pharmacology*; D010870:Pineal Gland / Q000502:physiology*; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000473:pathology*; D014176:Protein Biosynthesis
D010455:Peptides; C016320:polypeptide pineal extract