[Repair of embichin-induced DNA damage in embryonal fibroblasts of 101/H and CBA mice].
The changes in the molecular weight of DNA one-strand fragments were studied after a short-term incubation of 101/H and CBA mouse cells with embichin (nitrogen mustard). Unlike CBA mouse cells in which the repair of DNA lesions is completed after 24 hours, DNA in 101/H mouse cells was shown to be strongly fragmented. It is assumed that the deficiency of the repair of DNA lesions induced by embichin in 101H/ mouse cells is due to the deficiency of the last stage of excision repair, namely of the ligase reaction or to the uncoordinated pattern of the endonuclease and ligase reactions.
D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000032:analysis; D005347:Fibroblasts / Q000378:metabolism; D008466:Mechlorethamine / Q000494:pharmacology*; D051379:Mice; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D013045:Species Specificity
D000477:Alkylating Agents; D004277:DNA, Single-Stranded; D008466:Mechlorethamine
Reparatsiia povrezhdeniĭ DNK, indutsirovannykh émbikhinom, v émbrional'nykh fibroblastakh mysheĭ liniĭ 101/H i CBA.