[Production and extracellular arrangement of proteoglycans in the growing hyaline cartilage].
Fixation of hyaline cartilage with potassium ferricyanide in addition to glutaraldehyde and osmium tetroxide leads to a better stabilization of the glycosaminoglycans. The resulting chelate formation enhances the electronmicroscopic density. In active chondrocytes electron dense material is found intracellularly in the rough endoplasmic reticulum, in transport vesicles, in the Golgi apparatus and in big vacuoles. Intercellularly large amounts of the glycosaminoglycans are preserved. The correlations between the macromolecules and the collagenous fibrills are demonstrated.
D000818:Animals; D002356:Cartilage / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D005110:Extracellular Space / Q000032:analysis; D005404:Fixatives; D008854:Microscopy, Electron; D009940:Organoids / Q000648:ultrastructure; D011509:Proteoglycans / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D014617:Vacuoles / Q000032:analysis; D014989:Xiphoid Bone
D005404:Fixatives; D011509:Proteoglycans
Produktion und extracelluläre Anordnung von Proteoglykanen im wachsenden hyalinen Knorpel.