title: Evidence against the reported antithiamine effect of caffeic and chlorogenic acids.
abstract: Contrary to earlier literature reports, chlorogenic acid (I) is shown to have little or no deleterious effect on thiamine (II) when the 2 compounds are codissolved in aqueous solution. Evidence from nmr spectroscopy and from t. l. c. determination demonstrate that when II is heated at 60 degrees C and at pH 7.8 for 24h, about 10% is destroyed. This result is the same whether I is present or not. Incubating II with I or with caffeic acid (III) in a phosphate buffer at pH 7 or in thiamine assay medium at pH 6 for up to 24h at 37 degrees C did not reduce the growth activity of II or Lactobacillus viridescens. We conclude that I and III are not antithiamine agents.
mesh_terms: D002109:Caffeic Acids / Q000494:pharmacology*; D002726:Chlorogenic Acid / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D002934:Cinnamates / Q000494:pharmacology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D013831:Thiamine / Q000037:antagonists & inhibitors*
chemical_list: D002109:Caffeic Acids; D002934:Cinnamates; D002726:Chlorogenic Acid; D013831:Thiamine