Identification of fatty acid synthetase messenger RNA on free polyribosomes isolated from lactating rabbit mammary gland.
The synthesis of fatty acid synthetase on free polyribosomes from lactating rabbit mammary gland was demonstrated by using polyribosomes run-off techniques and immunochemical identification of products with synthetase antiserum. Several reproducible and discrete immunoprecipitable polypeptides were observed which were within the molecular-weight range of the synthetase subunit (235 000--252 000), as well as several of lower molecular weight.
D000818:Animals; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D064429:Fatty Acid Synthases / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010455:Peptides / Q000032:analysis; D011132:Polyribosomes / Q000378:metabolism*; D011247:Pregnancy; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits
D010455:Peptides; D012333:RNA, Messenger; D064429:Fatty Acid Synthases