[Production of an erythrocytic ganglioside diagnostic reagent for detecting cholera enterotoxin and toxoid in indirect hemagglutination tests].
The studies described in this work have indicated that cholera enterotoxin and its components (cholerogenoid and subunit B) can be detected in amounts of 0.25, 0.28 and 0.6 microgram of protein per 1 ml, respectively, by means of erythrocytes sensitized with gangliozide-containing complex. The conditions for erythrocyte sensitization have been established. The methods of cholerogen titration in the passive hemagglutination test by means of the erythrocytic gangliozide diagnostic reagent and in Craig's skin test have been shown to correlate. Similarly, the passive hemagglutination test is supposed to be suitable for detecting other bacterial toxins interacting with gangliozides.
D000818:Animals; D001923:Brain Chemistry; D002772:Cholera Toxin / Q000032:analysis*; D004768:Enterotoxins / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology; D005732:Gangliosides / Q000302:isolation & purification; D006386:Hemagglutination Tests / Q000379:methods; D012882:Skin Tests; D013552:Swine; D014121:Toxoids / Q000032:analysis*; D014734:Vibrio cholerae / Q000032:analysis*
D004768:Enterotoxins; D005732:Gangliosides; D014121:Toxoids; D002772:Cholera Toxin
Poluchenie éritrotsitarnogo gangliozidnogo diagnostikuma dlia vyiavleniia kholernogo énterotoksina i toksoida v reaktsii nepriamoĭ gemagglutinatsii.