title: Erythropoietic differentiation in humans: in vitro studies on erythroid progenitors and Hb synthesis in fetal, newborn and adult life.
abstract: Human erythroid progenitors from fetal liver, cord or adult blood and adult marrow were cultured in methylcellulose, according to standard techniques. Their clonogenetic features (colony morphology and number, time/growth curve, erythropoietin (Ep) and burst-enhancing factor (BEF) sensitivity, in vitro 3H-thymidine suicide index, etc) were comparatively investigated. Three classes of fetal liver erythroid progenitors (primitive or intermediate BFU-E, CFU-E) have been thereby identified and characterized. Furthermore, globin chains (alpha, beta, G gamma, A gamma) synthesis has been evaluated in single erythroid colonies, either well-or poorly-hemoglobinized, by means of a novel technique including analytical iso-electric focusing (IEF), sometimes preceded by preparative IEF separation of HbF and HbA. On the basis of these results, a model for the regulation of Hb synthesis is proposed here.
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells; D004920:Erythropoiesis; D005260:Female; D005333:Fetus; D005914:Globins / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006454:Hemoglobins / Q000096:biosynthesis*; D006801:Humans; D007231:Infant, Newborn; D008099:Liver / Q000166:cytology; D011247:Pregnancy
chemical_list: D006454:Hemoglobins; D005914:Globins