[Study of the fertilization capacity of mouse ova activated by thermal shock].
The possibility of fertilization and development of polyspermia was studied in mouse oocytes stimulated to development with thermal shock. Fertilization was done using non-incubated and incubated (capacitated) spermatozoa. The experiment was carried out in several versions. It was observed that thermal shock caused different types of oocyte stimulation. The type of stimulation played no role in the process of spermatozoa penetration into the oocytes. The capacitated spermatozoa had a greater ability of penetration into the stimulated oocytes than non-capacitated ones. The possibility of spermatozoa penetration into stimulated oocytes persisted up to 3 hours after stimulation, but they were not transformed into male pronucleus. The causes and mechanism of this observation are discussed. In the conclusion refertilization as the mechanism of dispermic triploidy has been ruled out.
D000818:Animals; D002478:Cells, Cultured; D004558:Electric Stimulation; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro; D006358:Hot Temperature; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa / Q000502:physiology; D013997:Time Factors
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