title: Reactivity of tumor-infiltrating, blood, spleen and lymph node lymphocytes against syngeneic glioma target cells.
abstract: Brain tumors were induced in inbred Fischer rats (F344) by administration of methylnitrosourea in the drinking water. One of the induced tumors, a pleomorphic glioma (78-219), was established in vitro and propagated as 78FR-G-219 permanent cell line. The tumorigenicity of the established line was investigated by intracerebral or subcutaneous inoculation of 1X10 (6) - 10X10(6) cells. Lymphocytes infiltrating secondary tumors (TIL) were enriched by a single step centrifugation on different discontinuous Percoll density gradients, while blood lymphocytes (PBL) and lymphocytes from spleen and lymph node (SL and LNL) were enriched by Ficoll - Isopaque flotation. The reactivity spectrum of the isolated lymphocytes raised against cultured 78FR-G-219 cells was monitored by means of two different assays: Lymphocyte microcytotoxicity test (LMC) and colony inhibition assay (CIA). Reactivity of PBL, SL and LNL of glioma-bearing animals was clearly reduced, while TIL showed no natural killer (NK) activity, no cytotoxicity against syngeneic 78FR-G-219 pleomorphic glioma cells and no colony inhibition in mixed lymphocyte/target cell cocultivation. NK activity of TIL was only slightly reduced against target cells of a non-cross-reacting syngeneic astrocytoma line (78FR-G-299).
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D002460:Cell Line; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic; D003602:Cytotoxicity, Immunologic; D005260:Female; D005910:Glioma / Q000139:chemically induced / Q000276:immunology*; D008198:Lymph Nodes / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D013154:Spleen / Q000473:pathology