title: Morphological transformation of C3H/10T1/2 CL8 cells by alkylating agents.
abstract: The standard method of the C3H/10T1/2 cell transformation assay cannot adequately detect alkylating agents. A modification of the standard procedure as described by Bertram and Heidelberger using a large number of synchronized cells and high levels of toxicity was evaluated for transformation using several alkylating agents. By using this method, N-methyl-N' -nitro-N-nitrosoguanidine (MNNG), beta-propiolactone (BPL), methyl methanesulfonate (MMS), methylnitrosourea (MNU) and 1,3-propane sultone (PS) transformed these cells. However, methyl iodide (MI) failed to induce any transformed foci.
mesh_terms: D000477:Alkylating Agents / Q000633:toxicity*; D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006847:Hydrocarbons, Iodinated / Q000633:toxicity; D008741:Methyl Methanesulfonate / Q000633:toxicity; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D008770:Methylnitrosourea / Q000633:toxicity; D051379:Mice; D011420:Propiolactone / Q000633:toxicity; D013876:Thiophenes / Q000633:toxicity
chemical_list: D000477:Alkylating Agents; D006847:Hydrocarbons, Iodinated; D013876:Thiophenes; D008769:Methylnitronitrosoguanidine; D008770:Methylnitrosourea; D011420:Propiolactone; D008741:Methyl Methanesulfonate; C014055:methyl iodide; C003218:1,3-propane sultone