Studies on human tonsillar lymphocyte membrane. III. Isolation and characterization of plasma membrane fractions after hypotonic lysis.
Hypotonic lysis was applied for the preparation of lymphocyte plasma membranes. By this method it was possible to separate membrane fractions with a high sialic acid to protein and cholesterol to phospholipid ratio. The plasma membrane fractions had a lower content of endoplasmatic reticulum than the preparations described earlier but were slightly contaminated with DNA. The isolation method proved to useful for the preparation of surface membranes from human lymphocytes
D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D002784:Cholesterol / Q000032:analysis; D004247:DNA / Q000032:analysis; D004721:Endoplasmic Reticulum; D006801:Humans; D007038:Hypotonic Solutions; D008214:Lymphocytes / Q000648:ultrastructure*; D014066:Palatine Tonsil / Q000166:cytology; D010743:Phospholipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis
D007038:Hypotonic Solutions; D010743:Phospholipids; D011506:Proteins; D012794:Sialic Acids; D004247:DNA; D002784:Cholesterol