title: Pretransplantation treatment of human teeth in tissue culture combined with storage in liquid nitrogen. A scanning electron microscopic study.
abstract: A combined method of tissue culture and storage in liquid nitrogen is described, which is suitable for covering the root surface of transplantable teeth with a dense cell layer: after an initial period of 10 days in tissue culture the teeth are stored in liquid nitrogen. 10 days before a transplantation the teeth are thawed and cultured again for 10 days. By this pretransplantation treatment a densely grown periodontal cell layer as well as an unlimited storage time are achieved.
mesh_terms: D046508:Culture Techniques; D006801:Humans; D008855:Microscopy, Electron, Scanning; D009584:Nitrogen; D014021:Tissue Preservation / Q000379:methods*; D014070:Tooth / Q000637:transplantation / Q000648:ultrastructure*
chemical_list: D009584:Nitrogen