Purification and properties of 3-methyladenine-DNA glycosylase from L-cells.
3-Methyladenine-DNA glycosylase from L-cells has been purified approximately 800-fold. The enzyme is present primarily in the nucleus of the cells. The enzymatic reaction was sensitive to changes in the assay conditions and optimum activity was found at pH 6.5 and at 100 mM KCl. Mg2+ did not effect the enzymatic reaction, which also worked in the presence of EDTA. The activity on denatured methylated DNA was 20-40% of that of the native double-stranded form. Sephadex gel filtration of the most purified fraction revealed enzyme species with molecular weights of 68000, 47000 and 27000, which differ from those reported for corresponding enzymes from other organisms. Addition of the product, 3-methyladenine, to the reaction mixture resulted in inhibition of the glycosylase activity of up to 60%. The remaining activity could not be abolished by increasing the concentration of 3-methyladenine.
D000225:Adenine / Q000031:analogs & derivatives / Q000378:metabolism; D000818:Animals; D045647:DNA Glycosylases; D004279:DNA, Viral / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007739:L Cells / Q000201:enzymology*; D008745:Methylation; D051379:Mice; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D013379:Substrate Specificity
D004279:DNA, Viral; C025946:3-methyladenine; D011189:Potassium Chloride; C021565:3-methyladenine-DNA glycosylase; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D000225:Adenine