[Inhibition of creatine kinase activity by glycolysis metabolites].
It is shown that lactate is a noncompetitive inhibitor of muscle creative kinase Ki 16 mmol with respect to MgATP. When inhibitors are applied in combination (lactate with glucose-6-phosphate or inorganic phosphate, glucose-6-phosphate with Pi or all three inhibitors together) a competition arises. When applied together the combined action is weaker than the sum of inhibition of three effectors in the case of separate application. The competition is revealed in model experiments with metabolite concentrations peculiar to resting and acting muscle. The supposition is made that formation of lactate, glucose-6-phosphate and inorganic phosphate in the muscle tissue can induce partial, but not complete inhibition of the creatine kinase activity.
D000818:Animals; D001667:Binding, Competitive; D003402:Creatine Kinase / Q000037:antagonists & inhibitors*; D019298:Glucose-6-Phosphate; D005958:Glucosephosphates / Q000494:pharmacology*; D006019:Glycolysis; D007700:Kinetics; D007773:Lactates / Q000494:pharmacology*; D019344:Lactic Acid; D009132:Muscles / Q000201:enzymology*; D010710:Phosphates / Q000494:pharmacology*; D011817:Rabbits
D005958:Glucosephosphates; D007773:Lactates; D010710:Phosphates; D019344:Lactic Acid; D019298:Glucose-6-Phosphate; D003402:Creatine Kinase
Ingibirovanie aktivnosti kreatinkinazy metabolitami glikoliza.