Red cell ATP and 2,3-diphosphoglycerate concentrations as a function of dihydroxyacetone supplementation of CPD adenine.
Units of CPDA-1 whole blood were subdivided and each treated with additions of dihydroxyacetone (DHA) to give final concentrations from 0 to 80 mM. The 'optimum' concentration of DHA to maintain 2,3-diphosphoglycerate (2,3-DPG) with minimal loss of ATP during 42 days of storage appeared to be 30 mM of DHA. With this formulation, red cell 2,3-DPG concentrations rose to 130-140% of normal by 14 days and then decreased in a near-linear manner to 50-60% normal by 42 days, while maintaining adequate ATP levels. In addition, packed red cells were prepared form CPD fresh blood and treated with adenine, glucose, and various concentrations (0-80 mM) of DHA. The cells also responded most favorably to 30mM DHA, although the response was not as positive as whole blood. This concentration of DHA produced nearly 100% maintenance of 2,3-DPG at 14 days with subsequent fall to 30% of normal by 42 days.
D019794:2,3-Diphosphoglycerate; D000225:Adenine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000097:blood*; D001793:Blood Preservation; D002951:Citrates / Q000494:pharmacology; D004098:Dihydroxyacetone / Q000494:pharmacology*; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D006400:Hematocrit; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000494:pharmacology; D014306:Trioses / Q000494:pharmacology*
D002951:Citrates; D004163:Diphosphoglyceric Acids; D010710:Phosphates; D014306:Trioses; D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate; D005947:Glucose; D000225:Adenine; D004098:Dihydroxyacetone