title: On determining the extent of side-pool synthesis in a three-pool model for whole body cholesterol kinetics.
abstract: Whole body cholesterol turnover is well described by a three-pool model. This model has eight unknown parameters: three masses, three synthesis rates, and two intercompartmental exchange rates. Only six parameters can be estimated by fitting the model to the plasma specific radioactivity-time curve which results from the intravenous injection of labeled cholesterol. Additional information is obtained if a precursor of cholesterol, labeled with a different isotope, is also injected. Equations are derived to enable the calculation of all eight model parameters from the two sum-of-exponentials equations that are fitted to the two tracer curves. The characteristics of a satisfactory precursor are discussed.
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis* / Q000097:blood; D006801:Humans; D007700:Kinetics; D008433:Mathematics; D008954:Models, Biological
chemical_list: D002784:Cholesterol