Affinity labeling of ribosomes from the livers of different vertebrates by 2-nitro-4-azidobenzoyl-phe-tRNA.
Ribosomal protein L 10 from the livers of trout, hen, and rat was found to be the main target for 2-Nitro-4-azidobenzoyl-Phe-tRNA in affinity labeling experiments. Therefore, despite somewhat different electrophoretic mobilities, this protein seems to be involved in the organization of the peptidyl transferase centre in ribosomes of various vertebrates.
D000345:Affinity Labels / Q000378:metabolism*; D000818:Animals; D001386:Azides / Q000378:metabolism*; D002645:Chickens / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis; D010458:Peptidyl Transferases / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl; D051381:Rats; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D014337:Trout / Q000378:metabolism
D000345:Affinity Labels; D001386:Azides; D012346:RNA, Transfer, Amino Acyl; D012269:Ribosomal Proteins; C033852:tRNA, 2-nitro-4-azidobenzoylphenylalanine-; D010458:Peptidyl Transferases