title: Gas-chromatographic determination of levamisole in human plasma-normalization and reliability of the method.
abstract: Levamisole has been used by many authors as an immunostimulant agent but the relationship between oral doses, plasma level and the therapeutic effects were not known. For such a study it was necessary to establish an accurate and reproducible method of measurement. We describe a modification of the gas chromatographic method of Wynants et al. (1975). The normalization of the extraction of the extraction process leads to greater reliability and accuracy (4 ng +/- 7.6%). Reproducibility was established using a series of 30 determinations from standard solutions and plasmas supplemented with Levamisole. The relative standard deviation in K evaluation was varied between 0.9 and 1.8%, 3.7 and 6.1% for standard solutions and plasmas respectively. Consequently this method was adopted for this pharmacokinetic study which was carried out on healthy and cancer patients.
mesh_terms: D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans; D007978:Levamisole / Q000097:blood*
chemical_list: D007978:Levamisole