[Stereospecific action of organothiophosphate amino acid derivatives on nervous system esterase activity in the cockroach].
The effect of optic isomers of Rp- and Sp-CH3(C2H5O)P(O)SCH2C(O)NHCH5-COOC2H5(EH-13), Rc- and Sc-(C2H5O)2P(O)SCH2C(O)NHCH (i-C3-H7)COOC2H5.(SH.-156), RpRc-, SpSc-, and SpRc-CH3(C2H5O)P(O) SCH2C(O)NHCH(i-C3H7)-COOC2H5(Sh.-142) on the fraction composition of esterases from the nervous tissue of American cockroach was investigated. Esterases were separated by polyacrylamide gel disc-electrophoresis. Sp-(EH-13) and Rc-(Sh.-156) were shown to inhibit carboxy esterase to a greater extent than their antipodes. Stereoisomers of the substance (Sh.-142) were found to influence activity of carboxy esterase molecular forms in a different way. It is postulated that the active surface of molecules of certain carboxy esterase isoenzymes may have stereospecific structure.
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D003058:Cockroaches / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D004950:Esterases / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D007306:Insecticides / Q000494:pharmacology*; D009420:Nervous System / Q000187:drug effects* / Q000201:enzymology; D009946:Organothiophosphorus Compounds; D010526:Periplaneta / Q000187:drug effects* / Q000201:enzymology; D013237:Stereoisomerism
D000596:Amino Acids; D007306:Insecticides; D009946:Organothiophosphorus Compounds; D004950:Esterases
Stereospetsifichnost' deĭstviia tiofosfororganicheskikh proizvodnykh aminokislot na ésteraznuiu aktivnost' nervnoĭ tkani.