Early RNA of adenovirus type 3 in permissive and abortive infections.
Early adenovirus type 3 cytoplasmic polyadenylated RNAs from HeLa and BHK-21 cells were detected and mapped on the viral genome by gel blotting and hybridization techniques. The sizes and locations of the 16 adenovirus type 3 RNAs were identical in the two cell types, although relative molarities of the various RNA species differed. Each of the early adenovirus type 3 RNAs was associated with polysomes in both cell types, suggesting that the abortive infection of hamster cells does not result from a defect in early adenovirus type 3 mRNA biosynthesis. No RNAs from regions transcribed late in infection of permissive cells were detected in BHK-21 cells.
D000256:Adenoviridae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004279:DNA, Viral / Q000096:biosynthesis; D005814:Genes, Viral; D006367:HeLa Cells; D006801:Humans; D009693:Nucleic Acid Hybridization; D011132:Polyribosomes / Q000032:analysis; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral