title: Dominance of an immune response by secondary cells: quantitation by allotype analysis.
abstract: Dominance of an immune response by secondary cells was demonstrated in a cell transfer system utilizing allotype-congenic mice. Spleen cells from BALB/c mice immunized with TNP-KLH were adoptively transferred into sublethally irradiation (200 R) congenic partners of a different allotype (C.B.-17 mice). Subsequent to the cell transfer, the recipients were challenged with TNP-KLH and bled periodically. Anti-TNP antibodies were isolated and analyzed for their content of donor and host IgG allotypes. Through day 58 after the adoptive transfer, 80% or more of the IgG antibody measured was of donor allotype. By day 110, most but not all recipients made IgG antibody principally of host allotype. In contrast, control C.B-17 recipients, which received nonimmune BALB/c spleen cells but otherwise treated as above, produced IgG antibodies almost entirely of host allotype. Total anti-TNP synthesis was reduced markedly in recipients of immune BALB/c cells so that there was a decrease of about 95% in the production of anti-TNP antibodies of host allotype, as compared with controls that received nonimmune cells. The results are discussed in terms of dominance of an immune response by secondary B cells.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D002874:Chromosome Mapping; D005799:Genes, Dominant; D006433:Hemocyanins / Q000276:immunology; D007116:Immunization, Passive; D007126:Immunoglobulin Allotypes / Q000235:genetics*; D007074:Immunoglobulin G; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D014301:Trinitrobenzenes / Q000276:immunology
chemical_list: D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D014301:Trinitrobenzenes; D006433:Hemocyanins