A novel immunization procedure for production of anti-cholecystokinin-specific antiserum of low cross-reactivity.
An anti-cholecystokinin octapeptide (CCK-8) antibody without cross-reactivity with pentagastrin, whose amino acid sequence is identical with the carboxy-terminal portion of CCK-8, was produced by immunization with CCK-8-keyhole limpet hemocyanin and injection of a conjugate of pentagastrin and a copolymer of D-glutamic acid and D-lysine to inhibit production of antibody cross-reacting with pentagastrin.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002417:Cattle; D002766:Cholecystokinin / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D007106:Immune Sera; D007114:Immunization / Q000379:methods*; D007118:Immunoassay; D007073:Immunoglobulin E; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010323:Passive Cutaneous Anaphylaxis; D010418:Pentagastrin / Q000276:immunology
D007106:Immune Sera; D007073:Immunoglobulin E; D002766:Cholecystokinin; D010418:Pentagastrin