title: Difference between magnesium and manganese ions in modification of effect of catecholamine on adenylate cyclase system in Ehrlich ascites tumor cells.
abstract: Involvement of divalent cations was examined in the activation of catecholamine sensitive adenylate cyclase in Ehrlich ascites tumor cells. In the presence of various concentrations of either Mg2+ or Mn2+, both basal and norepinephrine-stimulated, adenylate cyclase activities were measured. Both activities were gradually increased with the increase in Mg2+ concentration. On the other hand, with Mn2+, both activities were maximum at the low (2.5 mM) concentration, and decreased at the highest concentrations, the decrease being marked in the norepinephrine-stimulated activity. Similar difference between the two divalent cations was also observed in the effect on the binding of the beta-adrenergic ligand to the membrane receptor.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D002395:Catecholamines / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology*; D008345:Manganese / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology
chemical_list: D002395:Catecholamines; D008345:Manganese; D000262:Adenylyl Cyclases; D008274:Magnesium; D009638:Norepinephrine