Fibroblast cultures in experimental disturbed metabolic conditions. effects of anti-inflammatory drugs.
Fibroblasts in normal metabolic conditions constitute a simple cellular model for the study of anti-inflammatory drugs in culture, but do not take into account the factors related to the acute inflammatory reaction. A new approach was tried in submitting the fibroblasts to the action of a mouse peritoneal exudate or selected activated macrophages. Disrupted peritoneal material was inoculated to cultures in a volume representing a number of cells corresponding to 25 or 50% of fibroblast concentration at day 0 of the experimental time. Three anti-inflammatory drugs were retained to study their effects on proliferation and metabolic parameters: hydrocortisone, indomethacin and a newly-described molecule, the methoxybenzalthiosemicarbazone (MBT). At day 0, 1, and 2, fibroblasts were counted and total cellular protein and cellular glycolytic activity were determined. 4.5 X 10(-5) M hydrocortisone and MBT protected the fibroblast from the antiproliferative action of peritoneal exudate whereas in experiments using activated macrophage homogenate, all drugs exerted a protective and stimulatory effect.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology / Q000276:immunology*; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D002642:Chick Embryo; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D011506:Proteins / Q000378:metabolism; D012664:Semicarbazones / Q000494:pharmacology; D013997:Time Factors
D011506:Proteins; D012664:Semicarbazones; D006854:Hydrocortisone; D007213:Indomethacin