Effects of colchicine on gap junction formation during retinal neurogenesis.
Colchicine, injected into the amniotic cavity of 3 to 10 day-old chick embryos, has the following effects on the developing retina: 1. Cells in arrested metaphase accumulate in the ventricular portion of the matrix region. If colchicine is applied at the end of the first week of incubation, the kinetic migration of the cell nuclei is inhibited. 2. The cells lose their typical slender, bipolar shape to become ovoid to spherical. These morphological changes are more obvious when colchicine acts during the first week of incubation. 3. The formation of temporary gap junctions between the matrix cells of the retina occurs during the first week of development. When colchicine is administered around the 3rd day of incubation, the assembly of intramembranous particles (IMPs) during gap junctions formation is incomplete. These results suggest that an intact microtubular system within the submembranous cytoskeleton is essential for the assembly of these intercellular contacts.
D000818:Animals; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology*; D007365:Intercellular Junctions / Q000187:drug effects* / Q000648:ultrastructure; D008870:Microtubules / Q000187:drug effects; D009474:Neurons / Q000254:growth & development; D012160:Retina / Q000187:drug effects* / Q000196:embryology / Q000294:innervation / Q000648:ultrastructure; D013997:Time Factors
D003078:Colchicine