Morphological studies on the killing of schistosomula of Schistosoma mansoni by human eosinophil and neutrophil cationic proteins in vitro.
Purified eosinophil and neutrophil cationic proteins isolated from the lysosomal secretion granules of human granulocytes, evoke characteristic, dose-dependent morphological changes in young schistosomula of S. mansoni. The first sign of damage is seen with in 15-30 min of incubation and involves the formation of surface microvilli and blebs. Subsequently, tegumental evaginations of varying size are developed, but these appear to explode with rapidity, so that lengths of expanded tegumental outer membrane are deposited over the severely damaged surface of the parasite. Both types of granulocyte proteins are able to effect comparable damage at equimolar concentration. Other cationic proteins such as protamine and poly-L-arginine also damage the parasite surface but the pathological changes differ from those induced by the granulocyte proteins and they take longer to develop. In contrast, lysozyme-treated parasites are virtually similar to control schistosomula incubated in medium alone. These findings are discussed in relation to published data concerning the interaction of intact granulocytes with young schistosomula both in vitro and in vivo.
D000818:Animals; D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins / Q000494:pharmacology*; D002462:Cell Membrane / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D047091:Eosinophil Granule Proteins; D009113:Muramidase / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D011479:Protamines / Q000494:pharmacology; D012260:Ribonucleases; D012550:Schistosoma mansoni / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure*
D023181:Antimicrobial Cationic Peptides; D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D010455:Peptides; D011479:Protamines; C030509:neutrophil basic proteins; C015462:polyarginine; D012260:Ribonucleases; D009113:Muramidase