Relationship between chemical structure and cytotoxicity of aliphatic amines examined by a microtiter system with cultured fibroblasts.
Cytotoxicity of aliphatic amines was examined by a microtiter system with human diploid fibroblasts in relation to chain length of the compounds. Regression curves of the minimum inhibitory concentration on the carbon number in the chain was derived and it was revealed that the type of the regression curve of monoamines differed from that of diamines. Chromatographic hydrophobic parameter (RM) was also derived from high-performance thin-layer chromatography, and the relation between Hansch's parabolic model and the regression curve determined by carbon number (n) was discussed. The concept of quantitative structure-toxicity relationship is applicable to find less hazardous industrial chemicals. For the analysis of the relationship, measurement of RM value lessens the difficulty for obtaining partition coefficients of compounds. In addition, a newly developed microtiter system is simple, rapid and less tedious for the evaluation of the toxicity of chemicals especially in terms of a preliminary screening test.
D000588:Amines / Q000633:toxicity*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003959:Diamines / Q000633:toxicity; D005021:Ethylamines / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D013329:Structure-Activity Relationship
D000588:Amines; D003959:Diamines; D005021:Ethylamines