HPLC determination of antipyrine metabolites.
A high-performance liquid chromatography method is described which allows for the simultaneous determination of antipyrine, 4-hydroxyantipyrine, norantipyrine and 3-hydroxyantipyrine. Hydrolysis conditions with respect to source glucuronidase, amount of glucuronidase, pH of incubation and addition of antioxidant to the incubation medium proved to be very critical for norantipyrine and to a lesser extent 4-hydroxyantipyrine.  The method is of good sensitivity, accuracy and reproducibility. Thus, the method is well suited for detailed metabolic studies.
D000975:Antioxidants / Q000494:pharmacology; D000983:Antipyrine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D000077553:Edaravone; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D013997:Time Factors
D000975:Antioxidants; C007983:4-hydroxyantipyrine; D000077553:Edaravone; D000983:Antipyrine