Evaluation of blood toxicity in chronic uremia by an improved chromatographic method.
Biogel P2 gel chromatography of ultrafiltrate from chronic uremic patients leads to better resolution of the compounds present than so far reported in the literature. Reproducibility is good. Toxicity, evaluated by cell cultures, was detected in only one chromatographic fraction. Further purification of this fraction with HPLC on a RP18 column revealed two toxic fractions, to the better defined of which a polyol structure was ascribed.
D000818:Animals; D002642:Chick Embryo; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002908:Chronic Disease; D006801:Humans; D006435:Renal Dialysis; D014118:Toxins, Biological / Q000097:blood*; D014462:Ultrafiltration; D014511:Uremia / Q000097:blood*
D014118:Toxins, Biological