Culture of epithelial cells from bovine ruminal mucosa.
A method is reported for the primary in vitro culture of epithelial cells derived from bovine ruminal mucosa. That the cultures of ruminal epithelial cells consisted exclusively of stratum spinosum, stratum basale and stratum granulosum was confirmed by immunoperoxidase and immunofluorescence staining using carbonic anhydrase isoenzyme as a marker.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004847:Epithelial Cells; D008722:Methods; D009092:Mucous Membrane / Q000166:cytology; D012417:Rumen / Q000166:cytology*