Influence of puromycin and chloramphenicol on aminoketone synthesis ( delta-aminolevulinic acid synthesis) induction by allylisopropylacetamide in rat liver.
In vitro induction of aminoketone synthesis (AKS) by allylisopropylacetamide (AIA) in liver slices from 30 day-old male rats was reversibly inhibited by puromycin (50 micrograms/ml incubation mixture). Normal AKS was initially stimulated and thereafter blocked. Chloramphenicol (350 micrograms/ml) inhibited AIA mediated induction of AKS only partially and only when added 1 h before AIA to the incubation mixture. In vivo puromycin (3 i.p. injections of 15 mg/kg in 1 h intervals) did not influence AKS or AIA mediated AKS induction in newborn rats, whereas chloramphenicol (500 mg/kg s.c. 30 min prior to AIA) inhibited AIA mediated AKS induction in newborn rats. In 40 day-old rats pretreatment with chloramphenicol completely inhibited AKS induction by 200 mg/kg AIA.
D000624:5-Aminolevulinate Synthetase / Q000378:metabolism; D000081:Acetamides / Q000494:pharmacology*; D000502:Allylisopropylacetamide / Q000494:pharmacology*; D000622:Aminolevulinic Acid / Q000096:biosynthesis*; D000818:Animals; D000831:Animals, Newborn; D002701:Chloramphenicol / Q000494:pharmacology*; D004336:Drug Antagonism; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D007982:Levulinic Acids / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D011691:Puromycin / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
D000081:Acetamides; D007982:Levulinic Acids; D000502:Allylisopropylacetamide; D011691:Puromycin; D002701:Chloramphenicol; D000622:Aminolevulinic Acid; D000624:5-Aminolevulinate Synthetase