title: Influence of the lectins and polycation on the configuration of renal podocytes: a scanning electron microscopic study of renal podocytes after micropuncture of the glomerulus in vivo.
abstract: The lectins concanavalin A (Con A), wheat germ agglutinin (WGA), Ricinus communis agglutinin (RCA) I+II and the polycation protamine sulfate were applied directly to renal glomerular podocytes by micropuncture techniques in vivo; others received a control solution. To make visible the distribution of lectins, some rats were given fluorescein isothiocyanate-conjugated Con A. The glomeruli undergoing the micropuncture experiments were labeled and then prepared for SEM and TEM observation, in some cases also for histochemical analysis. Comparatively, the effect of application of the Con A and protamine sulfate solution by intraarterial infusion was studied. The glomeruli of a total of 100 Munich-Wistar rats were studied. Con A and WGA cause varying degrees of 'retraction' of the foot processes of the podocytes when applied using the techniques of micropuncture. Intraarterial infusion of a Con A solution, on the other hand, causes no changes in the podocytes. RCA II, applied for 10 min using micropuncture techniques, causes thickening and swelling of the foot processes as well as the formation of intercellular junctions ('agglutination'). RCA I, on the other hand, causes no changes in the podocytes of the rat glomerulus. Glomeruli treated with the micropuncture application of the polycation protamine sulfate demonstrate largely 'agglutination' and only sometimes localized minimal retraction of the foot processes of the podocytes. The intraarterial infusion of protamine sulfate causes almost exclusively 'agglutination' of the podocyte foot processes. Retraction of the podocyte foot processes is probably a result of the active movement of the podocytes, which in turn induced by attachment of lectines to the lectin receptors in glycocalyx of the podocyte cell membrane. Simple reduction of the polyanions in the podocyte cell membrane by protamine sulfate appears to cause only simple electrostatic interaction which then results in 'agglutination' of the podocyte foot processes.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D003712:Dendrites / Q000648:ultrastructure; D006651:Histocytochemistry; D007678:Kidney Glomerulus / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D037102:Lectins / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D010477:Perfusion; D011479:Protamines / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains
chemical_list: D037102:Lectins; D011479:Protamines