[Glycoconjugate biosynthesis in the arterial wall. VIII. Separation of molecular types in microsomal sialyltransferase].
Sialyltransferase activity of the intima-media of arterial walls is located in microsomes. The solubilization of enzyme by Triton X-100, purification by chromatography and isoelectric focusing lead to two isoenzymes (pHi 4.5 and 6.4), the second is widely predominant. Aortic sialyltransferase is sensitive to nucleotides, Mn++ dependent, and the affinity for substrate N-acetyl-neuraminic acid is 300 microM.
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D007525:Isoelectric Focusing; D046911:Macromolecular Substances; D008861:Microsomes / Q000201:enzymology*; D008970:Molecular Weight; D017830:Octoxynol; D011092:Polyethylene Glycols / Q000494:pharmacology; D012799:Sialyltransferases / Q000096:biosynthesis*; D014166:Transferases / Q000096:biosynthesis*
D046911:Macromolecular Substances; D011092:Polyethylene Glycols; D017830:Octoxynol; D014166:Transferases; D012799:Sialyltransferases
Biosynthèse des glycoconjugués dans la paroi artérielle. VIII - Séparation des variétés moléculaires de la sialyltransférase microsomique.