A comparison of the photochemical actions of 5- and 8-methoxypsoralen on CHO cells.
Chinese Hamster ovary (CHO) cells were treated with 5- or 8-methoxypsoralen (MOP) in combination with longwave UV light (UVA). The toxicity and the action of these substances on DNA synthesis as well as well as the nature of damage caused by them in combination with UVA light and the ability of the cells to repair this damage was examined. 8-MOP induces interstrand crosslinks within the DNA when applied together with UVA light. It decreases the rate of DNA synthesis at sublethal doses. 5-MOP shows the same effect though it induces virtually no DNA crosslinks. The repair of damage process. It is not quite complete at 24 h. Neither compound has an effect on CHO cells when applied without UVA light.
D000078223:5-Methoxypsoralen; D000818:Animals; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000096:biosynthesis; D004260:DNA Repair; D005260:Female; D066298:In Vitro Techniques; D008730:Methoxsalen / Q000633:toxicity*; D010053:Ovary / Q000166:cytology; D011701:PUVA Therapy / Q000009:adverse effects*; D010778:Photochemotherapy / Q000009:adverse effects*
D000078223:5-Methoxypsoralen; D004247:DNA; D008730:Methoxsalen