Biochemical changes in rat lungs after exposure to nitrogen dioxide.
Sixty-day-old male, specific pathogen-free rats were exposed continuously to 5 or 15 ppm NO2 for 1-7 d. Lung tissue from exposed and control rats was then analyzed for biochemical and enzymatic parameters. The exposure resulted in increased lung enzymatic activities, including elevated protein and DNA contents and nonprotein sulfhydryl levels. Biochemical and enzymatic parameters generally increased maximally after 4 d and remained elevated for up to 7 d of continued exposure. The magnitude of these increases was higher for 15 than for 4 ppm NO2. The increases in biochemical and enzymatic parameters may have occurred in response to NO2-induced lung injury.
D000818:Animals; D004247:DNA / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009585:Nitrogen Dioxide / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains
D011506:Proteins; D004247:DNA; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D009585:Nitrogen Dioxide