[Effect of the cultivation temperature on the growth and chemical composition of the methanol-assimilating yeast Candida boidinii].
The yeast Candida boidinii T2A was cultivated in a medium containing methanol or glucose as a sole carbon and energy source, and the effect of the cultivation temperature on the specific growth rate, the economic coefficient and the chemical composition of the yeast was studied in a continuous chemostat process. The specific rates of the yeast growth increase with the cultivation temperature up to optimal limits and then lower down abruptly in the supraoptimal zone. The maximal specific rate of the yeast growth in a medium with methanol at 30 degrees C is 0.24 h-1, while in a medium with glucose a 32 degrees C, it equals 0.38 h-1. In the suboptimal zone, the economic coefficient of the yeast growing in a medium with methanol at a dilution rate above 0.06 h-1 remains, for all practical purposes, at the same level (38-41%), while supraoptimal temperatures decrease the economic coefficient of the yeast. The results show a change in the content of protein and RNA as a function of the cultivation temperature and the dilution rate. It is concluded that the macromolecular composition of the yeast biomass is determined not only be the dilution rate, but also depends of temperature which regulates the rate of the yeast growth.
D002175:Candida / Q000032:analysis / Q000254:growth & development*; D055598:Chemical Phenomena; D002621:Chemistry; D003470:Culture Media / Q000378:metabolism; D000432:Methanol / Q000378:metabolism*; D013696:Temperature
D003470:Culture Media; D000432:Methanol
Vliianie temperatury kul'tivirovaniia na rost i khimicheskiĭ sostav metanolusvaivaiushchikh drozhzhe-ĭ Candida boidinii.