Identification of steroids produced by bovine chorion incubated with [14C]androstenedione.
Individual chorion taken from cows in early pregnancy (days 27, 44, 45 and 49) was incubated with [14C]androstenedione in short-term incubation in vitro. After preliminary extraction, separation and purification, metabolites were identified by recrystallization with authentic unlabelled steroids to constant specific activity. Major metabolites identified were 5 beta-androstane-3,17-dione (5 beta-androstanedione) and 5 beta-androstan-3 alpha-ol-17-one (aetiocholanolone) while minor metabolites were identified as 5 beta-androstane-3 alpha,17 beta-diol and 5 beta-androstan-17 alpha-ol-3-one. There was no 14C-labelled oestradiol or oestradiol detectable in the medium either as free oestrogens or as sulphates.
D000728:Androgens / Q000096:biosynthesis*; D000735:Androstenedione / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002823:Chorion / Q000378:metabolism*; D046508:Culture Techniques; D004958:Estradiol / Q000096:biosynthesis; D004970:Estrone / Q000096:biosynthesis; D005043:Etiocholanolone / Q000031:analogs & derivatives / Q000096:biosynthesis; D005260:Female; D011247:Pregnancy
D000728:Androgens; D004970:Estrone; D000735:Androstenedione; D004958:Estradiol; C006576:androstane-3,17-dione; D005043:Etiocholanolone