[Specific binding of cortisol to plasma protein in the guinea-pig from birth to weaning].
We have estimated two parameters of guinea-pig adrenal function during the neonatal period: --the concentration of plasma cortisol by competitive binding to protein; --the association constant, Ka, and total capacity of CBG binding (NS) by the thin-layer gel filtration technique. The concentration of plasma cortisol, very high at birth, declined markedly between neonatal days 1 and 10 and then remained constant between days 10 and 20, the time of weaning. Ka was 0.25.10(7).M-1 in the newborn and the adult of both sexes. This value was lower than that of other species. NS, which was very high at birth (111 +/- 5 micrograms/100 ml in the male; 101 +/- 5 micrograms/100 ml in the female), decreased rapidly during the first two neonatal days. At that time, it reached adult levels in the female (45 +/- 5 micrograms/100 ml) and remained stable. Adult binding capacity (16 +/- 2 micrograms/100 ml) was reached in the male after neonatal day 20.
D000818:Animals; D000831:Animals, Newborn / Q000097:blood*; D001798:Blood Proteins / Q000378:metabolism*; D002850:Chromatography, Gel; D005260:Female; D006168:Guinea Pigs; D006854:Hydrocortisone / Q000097:blood*; D007700:Kinetics; D008297:Male; D012737:Sex Factors; D014886:Weaning
D001798:Blood Proteins; D006854:Hydrocortisone
Liaison spécifique du cortisol aux protéines plasmatiques chez le cobaye, 'de la naissance au sevrage.