Combined use of the chemitrode with osmotic minipumps for experimental brain research.
This paper describes a simple-to-build minipump-chemitrode assembly which can be used for long term electrophysiological, chemical and behavioural studies. This system consists of an osmotic minipump, a cannula and an array of nine electrodes which are connected to a miniature Winchester Socket. A detailed plan for construction of both the chemitrode and the minipump-chemitrode is given together with some suggestions for possible usages in brain research(such as kindling). The osmotic minipump consists of a collapsible reservoir of flexible, impermeable material, surrounded by a sealed layer containing an osmotic agent--all of which is contained by a semipermeable membrane. Imbibed water generates hydrostatic pressure on the flexible lining of the reservoir, gradually compressing it, producing a constant flow of its contents through the delivery portal.
D000818:Animals; D001921:Brain / Q000187:drug effects*; D001931:Brain Mapping / Q000295:instrumentation*; D002415:Cats; D004594:Electrophysiology / Q000295:instrumentation; D006168:Guinea Pigs; D007696:Kindling, Neurologic; D008032:Limbic System / Q000187:drug effects; D008839:Microelectrodes; D008845:Microinjections / Q000295:instrumentation*; D009388:Neostigmine / Q000008:administration & dosage; D009482:Neurophysiology / Q000295:instrumentation*; D011817:Rabbits; D012110:Reserpine / Q000008:administration & dosage
D009388:Neostigmine; D012110:Reserpine