A multi-wavelength spectrophotometric method for the simultaneous determination of five haemoglobin derivatives.
A method is described by which the concentration of deoxyhaemoglobin, oxyhaemoglobin, carboxyhaemoglobin, haemoglobin and sulfhaemoglobin in a human blood sample is determined by passing the haemolysate without air contact through a coarse filter and subsequently measuring the absorbance at lambda = 500, 569, 577, 620 and 760 nm. The ensuing set of equations is solved by matrix calculation with the aid of a simple computer program. The method has been tested by comparing it with conventional methods for the determination of the various haemoglobin derivatives separately.
D002263:Carboxyhemoglobin / Q000032:analysis; D003201:Computers; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008433:Mathematics; D010108:Oxyhemoglobins / Q000032:analysis; D013053:Spectrophotometry / Q000379:methods*; D013435:Sulfhemoglobin / Q000032:analysis
D006454:Hemoglobins; D010108:Oxyhemoglobins; D013435:Sulfhemoglobin; D002263:Carboxyhemoglobin