Inhibition of molybdenum blue formation by ATP.
Molybdenum blue formation was not affected by the presence of ATP up to a concentration of 1.2 muM/l. At higher concentrations the color development was inhibited relative to ATP concentration, finally reaching complete inhibition. Auto-hydrolysis of ATP was found at a rate of 1.4%/h. An exact determination of inorganic phosphate in the presence of easily hydrolyzed phosphate esters requires the measurement of extinction at fixed time intervals and extrapolation back to time zero.
D000255:Adenosine Triphosphate; D007202:Indicators and Reagents; D007700:Kinetics; D008982:Molybdenum; D010710:Phosphates
D007202:Indicators and Reagents; D010710:Phosphates; C017541:molybdenum blue; D008982:Molybdenum; D000255:Adenosine Triphosphate