An electron microscopic radioautographic study of collagen secretion in periodontal ligament fibroblasts of the mouse: II. Colchicine-treated fibroblasts.
Colchicine administered intravenously depolymerized microtubules and disrupted the normal organization of the Golgi apparatus in periodontal ligament fibroblasts. Radioautography with 3H-proline indicated that collagen secretion was completely inhibited during a period of approximately 4 hours following the onset of the colchicine effect. During this period of secretory inhibition, labeled collagen precursors were present within a variety of dense bodies, primarily located in a juxtanuclear location replacing the normal Golgi complex. The time course of 3H-proline labeling from 2 to 8 hours suggested that small, newly formed dense bodies fused to form larger dense bodies and pleomorphic structures (zebra bodies), within which collagen precursors appeared to undergo partial polymerization. Autophagosomes, many labeled with 3H-proline, also increased in number after colchicine administration. A gradual decline in 3H-proline label occurred from 4 to 24 hours, presumably due to exocytosis of dense bodies or by the digestion of labeled collagen precursors within autophagosomes. These results support the concept that an intact microtubular network is essential for the organized transport of collagen precursors, from the rough endoplasmic reticulum to the Golgi apparatus, and the eventual transport and exocytosis of collagen secretory granules.
D000818:Animals; D001345:Autoradiography; D003078:Colchicine / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D010513:Periodontal Ligament / Q000187:drug effects* / Q000378:metabolism; D011392:Proline; D014316:Tritium
D014316:Tritium; D003094:Collagen; D011392:Proline; D003078:Colchicine