Fine structure of the cuticle of the black widow spider with reference to surface lipids.
The surface and transverse sections of the cephalothorax, abdomen, and walking leg cuticle of the black widow spider, Latrodectus hesperus, were examined by scanning and transmission electron microscopy. Cuticle that was untreated prior to normal EM preparative procedures was compared with cuticle subjected to lipid solvents and/or concentrated alkali. The surface of untreated dorsal cephalothorax cuticle contained droplets and a lipid film that obscured fine surface detail. Immersing the cuticle in chloroform: methanol removed the droplets and lipid film, exposing previously covered openings to dermal gland ducts. An epicuticle, exocuticle, and endocuticle were present in all transverse sections of cuticle as was a complex system of pore and wax canals that connected the epidermis with cuticle surface. The epicuticle of the walking leg was composed of three sublayers: outer membrane, outer epicuticle, and the dense homogeneous layer. A cuticulin layer was not observed. Lipid solvents did not significantly alter the morphology of any of these layers or the contents of the wax/pore canals.
D000005:Abdomen; D000818:Animals; D001740:Black Widow Spider / Q000032:analysis / Q000648:ultrastructure*; D002725:Chloroform; D004817:Epidermis / Q000648:ultrastructure; D005121:Extremities; D005260:Female; D008055:Lipids / Q000032:analysis*; D000432:Methanol; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013112:Spiders / Q000648:ultrastructure*
D008055:Lipids; D002725:Chloroform; D000432:Methanol