Procedure for isolation of the 20,000-dalton variant of human growth hormone.
An isolation procedure for the 20,000-dalton variant of human growth hormone has been devised to improve the yield of the final product. The improvement involved elimination of cumbersome steps that decreased yield, and modification of chromatography on DEAE-cellulose to provide better separation of the variant from the major form of growth hormone.
D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D013006:Growth Hormone / Q000302:isolation & purification*; D006801:Humans
D013006:Growth Hormone