title: Participation of inorganic phosphate in the chemiosmotic mechanism of mitochondrial energy transduction.
abstract: In respiring liver mitochondria in which Pi transport was inhibited and the intramitochondrial Pi value manipulated to vary about 3-fold, K+-ion uptake, H+-ion extrusion and stimulation of respiration were linearly related to the intramitochondrial Pi content. It is suggested that the HPO 2(4)-anion generated during respiration is the nondiffusible anion required for the Donnan distribution and membrane potential in mitochondria. The intramitochondrial H X HPO4-ionic species is the proton donor required for coupled respiration. The electroneural Pi-H+ cotransport closes the proton cycling in respiration.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004734:Energy Metabolism; D006859:Hydrogen / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D010710:Phosphates / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D013324:Strontium / Q000494:pharmacology; D014634:Valinomycin / Q000494:pharmacology
chemical_list: D010710:Phosphates; D014634:Valinomycin; D006859:Hydrogen; D011188:Potassium; D002118:Calcium; D013324:Strontium