Comparative study of the kinetics of 3-hydroxy-3-methylglutaryl coenzyme A reductase and [14C]-acetate incorporation into cholesterol in human lymphocytes stimulated by phytohemagglutinin or sterol efflux.
The time course of sterol biosynthesis was compared after two different stimulations in normal human lymphocytes: culture either in the presence of phytohemagglutinin (PHA) or in a lipid-depleted medium (i. e. a condition which produces sterol efflux). Stimulation by PHA gives rise to an acute and rapid response in 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase activity and cholesterol synthesis. The sterol efflux induction produces a slower and milder response and the delayed sterol production consists mainly of lathosterol.
D000085:Acetates / Q000378:metabolism; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis*; D003470:Culture Media; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000097:blood*; D007700:Kinetics; D008074:Lipoproteins / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013261:Sterols / Q000096:biosynthesis
D000085:Acetates; D003470:Culture Media; D008074:Lipoproteins; D010835:Phytohemagglutinins; D013261:Sterols; C001521:lathosterol; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases