title: Alteration of the circulating life and antigenic properties of bovine adenosine deaminase in mice by attachment of polyethylene glycol.
abstract: Polyethylene glycol was attached covalently to adenosine deaminase (ADA) using cyanuric chloride as the coupling agent. The modified adenosine deaminase (PEG-ADA) appears to lose its immunogenicity in mice following multiple intravenous injections. PEG-ADA does not react with antibodies raised against native ADA. The circulating half-life (T1/2) of PEG-ADA was increased to 28 hr. The lack of detectable antibody formation and long circulating life may make PEG-ADA suitable for treating human ADA deficiency.
mesh_terms: D000243:Adenosine Deaminase / Q000097:blood / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D002417:Cattle; D003432:Cross-Linking Reagents; D006207:Half-Life; D051379:Mice; D009700:Nucleoside Deaminases / Q000276:immunology*; D011092:Polyethylene Glycols / Q000097:blood / Q000276:immunology*; D014227:Triazines
chemical_list: D000941:Antigens; D003432:Cross-Linking Reagents; D014227:Triazines; D011092:Polyethylene Glycols; C019309:cyanuric chloride; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase