Immunoglobulin-producing cells in CSF and blood from patients with multiple sclerosis and other inflammatory neurological diseases enumerated by protein-A plaque assay.
Using the Protein-A plaque assay, numbers of IgG + IgA + IgM producing cells determined in patients with multiple sclerosis (MS) were 0.1-5% in CSF and 0.1-0.7% in peripheral blood; interestingly, 7 of 11 MS patients had IgM producing cells in CSF. In patients with aseptic meningitis (AM), the corresponding values were 0.04-7.5% in CSF and 0.4-2.4% in peripheral blood. There were more Ig producing cells in peripheral blood from patients with AM and MS than in healthy subjects. A correlation between numbers of IgG producing cells in CSF and the concentrations of intrathecally produced IgG (CSF IgG index) was registered in patients with AM; the same was the true for IgA. The Protein-A plaque method, adopted for 20 X 10(3) lymphocytes, makes possible enumeration of Ig-producing cells in CSF and discrimination among cells secreting different Ig classes, thereby being a powerful tool for studying immune reactions in the CNS-CSF compartment.
D000328:Adult; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D007249:Inflammation / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism; D008214:Lymphocytes / Q000032:analysis*; D008297:Male; D008581:Meningitis / Q000134:cerebrospinal fluid*; D008582:Meningitis, Aseptic / Q000097:blood / Q000134:cerebrospinal fluid* / Q000378:metabolism; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid* / Q000378:metabolism; D009422:Nervous System Diseases / Q000097:blood / Q000134:cerebrospinal fluid* / Q000378:metabolism; D010950:Plasma Cells / Q000032:analysis*; D013205:Staphylococcal Protein A; D010948:Viral Plaque Assay
D007136:Immunoglobulins; D013205:Staphylococcal Protein A