The area of lymphocyte nucleoli, a new method estimating the blastic transformation of stimulated lymphocytes in in vitro cultures.
To estimate the degree of lymphocyte reactivity in patients with internal diseases with immune components, the authors used a new method of investigation, the area of lymphocyte nucleoli (ALN), recently proposed by them. ALN was determined in blood cultures stimulated with phytohemagglutinin (PHA) and sacrificed after three days. The blood was obtained from 120 patients with chronic internal diseases with immune components such as: bronchial asthma, chronic hepatitis, rheumatoid arthritis, systemic lupus erythematosus or chronic lymphocytic leukemia (CLL). The data obtained showed clearly that, compared with the classical test of lymphocyte blastic transformation, ALN--which presented increases even by more than 100% over normal in rheumatoid arthritis and in systemic lupus erythematosus--can supply additional information regarding the degree of reactivity of the cultivated lymphocytes. In CLL, as it was to be expected, ALN values were below the normal.
D001172:Arthritis, Rheumatoid / Q000276:immunology; D001249:Asthma / Q000276:immunology; D002466:Cell Nucleolus; D002478:Cells, Cultured; D006505:Hepatitis / Q000276:immunology; D006801:Humans; D007154:Immune System Diseases / Q000097:blood*; D037102:Lectins / Q000494:pharmacology; D007945:Leukemia, Lymphoid / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology* / Q000648:ultrastructure
D037102:Lectins