title: Antiproliferative activity of protocatechualdehyde on Chinese hamster cells grown in culture.
abstract: The mode of cytotoxic action of protocatechualdehyde on V79 cells was examined. The cytotoxicity of protocatechualdehyde on V79 cells was time- and cell cycle-dependent. The lethal action is selective for the cells at S phase only. The cells lethally damaged at S phase seemed to complete their DNA synthesis, although the rate of DNA synthesis became lower and the duration of S phase was prolonged. Some cells arriving at G2 phase directly entered the next S phase without cell division and died of karyorrhexis or karyolysis. Cells treated at stages of the cell cycle other than S showed normal progression of their cell cycle to the G1/S boundary. The block point of the cell cycle seemed to lie a little behind that with hydroxyurea. When the cells at phases other than S were blocked with protocatechualdehyde for 14 hr, elongation of the next S phase occurred after removal of protocatechualdehyde. On alkaline sucrose gradient analysis, little scission of parental DNA was observed in the cells treated with protocatechualdehyde.
mesh_terms: D000818:Animals; D001547:Benzaldehydes / Q000494:pharmacology*; D002396:Catechols / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000096:biosynthesis; D006918:Hydroxyurea / Q000494:pharmacology; D007399:Interphase / Q000187:drug effects; D008168:Lung; D013997:Time Factors
chemical_list: D001547:Benzaldehydes; D002396:Catechols; C005581:protocatechualdehyde; D004247:DNA; D006918:Hydroxyurea