Induction of diphtheria toxin-resistant mutants in human cells by ultraviolet light.
Stable spontaneous mutants resistant to the protein synthesis inhibitor diphtheria toxin (DT) have been selected in human cell line EUE at a very low frequency (less than 8 x 10(-6)). U.v.-induced mutation has been quantitatively measured: treatment of cells with u.v. light increased the frequencies of diphtheria toxin resistant (DTr) mutants up to 1000-fold. The maximum recovery of DTr mutants was observed after a short expression period, for all u.v. doses tested, and was followed by a decrease in mutation frequency on subsequent passages.
D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004167:Diphtheria Toxin / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D004351:Drug Resistance / Q000528:radiation effects; D004848:Epithelium; D006801:Humans; D009132:Muscles / Q000196:embryology; D009154:Mutation; D012867:Skin / Q000196:embryology; D014466:Ultraviolet Rays
D004167:Diphtheria Toxin