A rapid experimental method to study primary embryonic induction.
The technique described here is a combination of the sandwich-method and cell culture. It allows one to know quickly whether induction occurred or not (48 h if cell spreading is considered, 4-5 days if morphological differentiation is observed). The dissociation of the explants soon after induction does not disturb their inductive pattern. Moreover, this method allows a daily observation of the morphological events and study at the cellular and/or molecular level.
D000818:Animals; D002449:Cell Aggregation; D002454:Cell Differentiation; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods; D005260:Female; D005775:Gastrula / Q000166:cytology / Q000502:physiology*; D009432:Neural Crest / Q000166:cytology / Q000502:physiology; D010999:Pleurodeles