Removal of hepatitis B virus from a concentrate of coagulation factors II, VII, IX and X by hydrophobic interaction chromatography.
Deliberately added hepatitis B surface antigen (HBsAg) and hepatitis B virus (HBV) could be removed by hydrophobic interaction chromatography from a concentrate of coagulation factors II, VII, IX and X. Chromatography in high concentrations of salt, preferably on octanoic acid hydrazide-Sepharose 4B, resulted in 104 -105 -fold reduction of HBsAg. The binding properties of HBsAg and HBV to the hydrophobic gels were similar and the chromatographic method seems particularly useful in removing low concentrations of virus-related material from protein solutions. The yield of protein from the coagulation factor concentrate was about 85% and the material showed the same stability and biological activities as before the chromatography.
D000327:Adsorption; D001779:Blood Coagulation Factors / Q000302:isolation & purification*; D002210:Caprylates; D002847:Chromatography, Agarose; D005164:Factor IX / Q000302:isolation & purification; D005167:Factor VII / Q000302:isolation & purification; D005170:Factor X / Q000302:isolation & purification; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D011516:Prothrombin / Q000302:isolation & purification
D001779:Blood Coagulation Factors; D002210:Caprylates; D006514:Hepatitis B Surface Antigens; C033463:octanoic acid hydrazide; D005167:Factor VII; D011516:Prothrombin; D005164:Factor IX; D005170:Factor X