[Separation of a mixture of L-isoleucine and L-leucine].
The conditions of L-leucine and L-isoleucine esterification were investigated. It was found that chromatographically homogeneous L-isoleucine and L-leucine could be isolated from their mixture during its incomplete esterification. As a result, L-leucine transformed into L-leucine and ester chlorohydrate L-isoleucine into L-isoleucine chlorohydrate. By means of further neutralization of the reaction mixture L-isoleucine with a 80-85% yield was obtained, and with the aid of L-leucine ester hydrolysis L-leucine with a 80-85% yield was produced. The content of the major amino acid following its crystallization from the water-ethanol mixture was 99%.
D002855:Chromatography, Thin Layer; D004338:Drug Combinations; D004951:Esterification; D006863:Hydrogen-Ion Concentration; D007532:Isoleucine / Q000302:isolation & purification*; D007930:Leucine / Q000302:isolation & purification*; D013696:Temperature; D013997:Time Factors
D004338:Drug Combinations; D007532:Isoleucine; D007930:Leucine
Razdelenie smesi L-izoleĭtsina i L-leĭtsina.