title: Effect of human saliva on the fluoride sensitivity of glucose uptake by Streptococcus mutans.
abstract: The fluoride (F) sensitivity of glucose uptake by whole cell suspensions of streptococcus mutans in the presence and absence of human whole salivary supernatant was studied. It was observed that dithiothreitol (DTT) and other thiols markedly reduced the F sensitivity of cells when saliva (50%, vol/vol) was present during glucose uptake. In the absence of saliva, cells were sensitive to 2 to 2.5 mM F regardless of the presence of thiols. Supplementation of cells in phosphate or tris(hydroxymethyl)aminomethane-hydrochloride buffers with physiological concentrations of calcium or phosphate had no effect on the F sensitivity of the organism. Experiments with permeabilized cells suggested that thiols themselves had no direct effect on the F sensitivity of enolase (a principal F target). Cells pretreated with DDT subsequently exhibited decreased F sensitivity when examined in the presence of saliva but not in the absence of saliva. Cells pretreated with whole salivary supernatant were found to be subsequently less sensitive to F in the absence of saliva during glucose uptake. Furthermore, in cases where cells were pretreated with saliva, subsequent additions of DDT were unnecessary to obtain maximal reduction in the F sensitivity of glucose uptake. It was concluded that the saliva-dependent reduction in F sensitivity of glucose uptake was not due to sequestration of available F by salivary constituents. The data suggest that a salivary component(s) interacts directly with the microorganism in some manner which results in reduced F sensitivity of the process under study. Possible mechanisms of saliva action are discussed.
mesh_terms: D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D005459:Fluorides / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D010710:Phosphates / Q000494:pharmacology; D010766:Phosphorylation; D012463:Saliva / Q000382:microbiology*; D013295:Streptococcus mutans / Q000187:drug effects*
chemical_list: D010710:Phosphates; D005947:Glucose; D005459:Fluorides; D002118:Calcium; D004229:Dithiothreitol