Healing-over in rat crystalline lens.
1. 42K efflux has been studied in normal and injured rat crystalline lenses to test their ability to heal over. Following injury, a sizeable, but transient, increase in fractional loss of 42K takes place. 2. The lens healing-over is Ca-dependent and cannot be accounted for by membrane resealing. This is excluded by the fact that Procion Yellow gains access to damaged fibres even 2 hr after injury (when the healing-over is completed). 3. The occlusion of the junctional channels is the basic mechanism of healing-over. This is supported by the observation that Procion Yellow does not diffuse from damaged to intact fibres and by the Ca dependency of the phenomenon.
D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000502:physiology; D066298:In Vitro Techniques; D007908:Lens, Crystalline / Q000378:metabolism / Q000502:physiology*; D008566:Membranes / Q000502:physiology; D011188:Potassium / Q000378:metabolism; D051381:Rats; D014945:Wound Healing
D011188:Potassium; D002118:Calcium