title: Purification of lymphocystis disease virus (LDV) grown in tissue culture. Evidences for the presence of two types of viral particles.
abstract: Lymphocystis disease virus was highly purified from host cells by precipitation with PEG-6000 and isopycnic centrifugation in a metrizamide gradient. Metrizamide gradient centrifugation produce two distinct bands at equilibrium. As calculated from reconstruction experiments, only 4 and 0.3% respectively of the host DNA and the host proteins were recovered at the position of the bands. The final recovery of infectivity was about 41%. Electron microscopy of the bands showed two types of particles: small and dense particles measuring 100-150 nm and lymphocystis virions that measured about 300-350 nm in diameter.
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D004267:DNA Viruses / Q000302:isolation & purification* / Q000648:ultrastructure; D005393:Fish Diseases / Q000382:microbiology*; D005399:Fishes; D008218:Lymphocytosis / Q000382:microbiology / Q000662:veterinary*; D008854:Microscopy, Electron