title: Esterase-16 (es-16): characterization, polymorphism, and linkage to chromosome 3 of a kidney esterase locus of the house mouse.
abstract: A polymorphism for an isozyme of a presumed arylesterase, esterase-16 (EC 3.1.1.2), has been detected in kidney, heart, and spleen of the house mouse, Mus musculus, by means of isoelectric focusing and by disc electrophoresis. Three phenotypes can be distinquished: the ES-16A phenotype (IEP 5.9) was found in C57BL/10Sn and many other laboratory inbred strains; the ES-16B phenotype (IEP 6.1) was found in M. m. molossinus; and the ES-16C phenotype (IEP 5.9; very weak activity) was found in Peru-Coppock. Esterase-16 is strongly inhibited by 10(-3) M p-chloromercuribenzoate, but not by 2. 10(-4) M bis-p-nitrophenyl phosphate or by 10(-3) M Diamox. It stains well with indoxyl acetate and other indigogenic substrates but only weakly with alpha-naphthyl acetate. Esterase-16 is completely insoluble in water. It is apparently governed by a structural gene locus, Es-16, with three alleles, Es-16a, Es-16b, and ES-16c, respectively. Es-16 is closely linked to Car-1 and Car-2 on chromosome 3 Typing of 94 animals of the backcross (C57BL/10Sn x M. m. mol.) F1 x M. m. mol. revealed a recombination frequency of 8.51 /+- 2.9%.
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D004950:Esterases / Q000235:genetics*; D005260:Female; D008040:Genetic Linkage; D007526:Isoelectric Point; D007668:Kidney / Q000201:enzymology; D008297:Male; D051379:Mice / Q000235:genetics*; D008815:Mice, Inbred Strains / Q000235:genetics; D009206:Myocardium / Q000201:enzymology; D011110:Polymorphism, Genetic; D013154:Spleen / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D004950:Esterases