[Reassociation of rat DNA fractions].
A method for direct registration of the first derivative of DNA reassociation kinetics in spectrophotometric cuvettes is proposed. Using this method, the rat liver DNA obtained by chromatography on hydroxyapatite was studied. The differential curves of reassociation and changes in hyperchromic spectra revealed the presence in the rat genome of 4 types of repeating sequences differing in their GC-content. A scheme of preparative separation of DNA with respect to the reassociation rate was developed. Three types of sequences repeating 3.10(5), 600 and 1 times in the genomes with the GC-content of 50, 42, and 33%, respectively, were obtained in a homogeneous state. The advantages of the differential method of registration of the kinetic curves for the study of DNA structure and calculation of fractionation schemes are discussed.
D000818:Animals; D004247:DNA / Q000302:isolation & purification; D007700:Kinetics; D008099:Liver / Q000032:analysis*; D008433:Mathematics; D009695:Nucleic Acid Renaturation; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D004247:DNA
Analiz reassotsiatsii fraktsii DNK krysy.