A logical approach to the isolation of lactate dehydrogenase isozyme X from human testes: a general rationale for the isolation of homotetrameric LDH isozymes.
Immunoadsorbent and oxamate-Sepharose chromatography were used to isolate electrophoretically homogeneous LDH-X from human testes with a final specific activity of 125 IU/mg and good yields: other applications of this appraoch are discussed.
D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007164:Immunosorbents; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification*; D008297:Male; D008722:Methods; D013737:Testis / Q000201:enzymology*
D007164:Immunosorbents; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase