Uptake of D-glucose and L-proline by oligotrophic and heterotrophic marine bacteria.
The transport systems of the oligotrophic bacterium 486 for D-glucose and L-proline have been compared with those of the heterotrophic bacterium RP-303. Kinetic studies demonstrated that the rates of D-glucose and L-proline uptake by the two organisms were saturable processes. The apparent Km values of strain 486 for D-glucose and L-proline were 13.0 microM and 0.2 microM, respectively, whereas those of strain RP-303 were 3.2 microM for D-glucose and 1.8 microM for L-proline. Competition studies indicated that the D-glucose transport system of each bacterium was highly specific for D-glucose. The L-proline transport system of the oligotrophic bacterium 486 had a broad specificity, whereas that of the heterotrophic bacterium RP-303 had a narrow one.
D000596:Amino Acids / Q000494:pharmacology; D001149:Arsenates / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D003847:Deoxyglucose / Q000378:metabolism; D004140:Dinitrophenols / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D007700:Kinetics; D011190:Potassium Cyanide / Q000494:pharmacology; D011392:Proline / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D012623:Seawater
D000596:Amino Acids; D001149:Arsenates; D004140:Dinitrophenols; C009277:sodium arsenate; D011392:Proline; D003847:Deoxyglucose; D005947:Glucose; D011190:Potassium Cyanide