Cerebellar tRNA methyltransferases: a developmental study.
Developmental patterns of homologous and heterologous tRNA methylation by cerebellar tRNA methyltransferases are described. The study revealed that: (a) homologous tRNA methylation results in the predominant formation of N2-methylguanine and 1-methyladenine; (b) tRNA methyltransferase of bulk-isolated Purkinje and granule cells methylate E. coli tRNAglu2 in vitro in a characteristic manner, and (c) the methylation of 8-day-old cerebellar, cortical and hepatic tRNA in vivo yields tRNAs containing different proportions of methylated bases. The findings suggest that the presumably cell-specific populations of cerebellar tRNA methyltransferases continue to alter their substrate recognition characteristics up to and beyond the first month of post-natal life.
D000375:Aging; D000818:Animals; D002531:Cerebellum / Q000201:enzymology*; D003596:Cytosine / Q000378:metabolism; D006147:Guanine / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008745:Methylation; D011689:Purkinje Cells / Q000201:enzymology; D051381:Rats; D012359:tRNA Methyltransferases / Q000378:metabolism*
D006147:Guanine; D003596:Cytosine; D012359:tRNA Methyltransferases