Partial purification and separation of H-2 antigens by free-flow preparative electrophoresis.
The membrane fraction of murine spleen cells was solubilized by autolysis in 0.25% potassium cholate and 3M potassium chloride, and the solubilized membrane components were fractionated by ultrafiltration and preparative free-flow electrophoresis. This procedure yielded partially purified fractions with enriched specific antigenic activities. The H-2Ka and H-2Da antigens were found to possess distinctly different electrophoretic mobilities.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000276:immunology; D004586:Electrophoresis / Q000379:methods*; D006183:H-2 Antigens / Q000302:isolation & purification*; D051379:Mice; D012995:Solubility; D013154:Spleen / Q000166:cytology*; D014462:Ultrafiltration / Q000379:methods
D006183:H-2 Antigens