title: The anomalous migration during two-dimensional gel electrophoresis of eukaryotic ribosomal proteins with oxidised thiol groups.
abstract: Two-dimensional gel electrophoresis of eukaryotic ribosomal proteins under conditions in which thiol groups are not maintained in a reduced state has been found to cause marked changes in the migration of certain proteins, apparently due to the formation of intramolecular disulphide bridges. The results suggest that certain previously designated eukaryotic ribosomal proteins are not, in fact, unique species.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney; D008623:Mercaptoethanol; D010084:Oxidation-Reduction; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D013438:Sulfhydryl Compounds
chemical_list: D012269:Ribosomal Proteins; D013438:Sulfhydryl Compounds; D008623:Mercaptoethanol