New androgen-stimulated proteins in the kidneys of female mice.
Testosterone administration to female mice for 25 days produced a 70% increase in total kidney protein in both A/J and C57BL/6J mice. This is in contrast to the known androgen-responsive proteins, such as beta-glucuronidase and alcohol dehydrogenase, which each represent less than 1% of the total kidney proteins even after maximum stimulation. To investigate this discrepancy, we initiated a study to identify major proteins which increase with androgen treatment. Three new cytoplasmic proteins designated T1, T2, and T3 were found in different subcellular fractions of both A/J and C57BL/6J mice. T1 (43,000 daltons) and T2 (60,000 daltons) were found in the mitochondrial-lysosomal fraction of 25-day androgen-treated mice. T3 (54,000 daltons) was found in the microsomal fraction. Each of these proteins increased several-fold during androgen treatment, so that they were easily identified using sodium dodecyl sulfate polyacrylamide gel electrophoresis. By contrast, no major changes were noted in the soluble proteins. A nonhistone chromosomal protein of 54,000 mol wt (T4) was found in chromatin preparations from androgen-stimulated A/J mice. Additional studies with androgen-insensitive Tfm/Y mice and with various hormones indicated that stimulation of the T proteins was dependent on androgenic steroid and a functional androgen receptor.
D000818:Animals; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005966:Glucuronidase / Q000096:biosynthesis; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008247:Lysosomes / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D008928:Mitochondria / Q000378:metabolism; D008970:Molecular Weight; D011374:Progesterone / Q000494:pharmacology; D014176:Protein Biosynthesis; D013739:Testosterone / Q000494:pharmacology*
D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D005966:Glucuronidase