Sodium ion/L-lactate co-transport in rabbit small-intestinal brush-border-membrane vesicles.
Uptake of L-lactate into rabbit jejunal brush-border-membrane vesicles prepared by a Ca2+-precipitation procedure was studied by a rapid filtration technique with L-[14C]-lactate as tracer. Transport of L-lactate into an intravesicular (osmotically reactive) space could be established. An inwardly directed NaCl gradient (outside 21 mM/inside 0mM) stimulated the uptake of L-lactate at 15 s 2-4-fold compared with that observed with an equal KCl gradient. A transient accumulation of L-lactate inside the vesicles (overshoot) was observed in the presence of an NaCl gradient. Gradients of LiCl, RbCl, CsCl or choline chloride were not able to replace NaCl in the stimulation of L-lactate uptake. L-Lactate uptake was saturable only in the presence of Na+. D-Lactate, DL-thiolactate (2-DL-mercaptopropionate), pyruvate and propionate inhibited the Na+-stimulated L-lactate uptake; D-lactate, thiolactate and pyruvate provoked trans-stimulation of L-lactate uptake. Artificially imposed diffusion potentials (inside negative) did not exert any effect on the Na+-dependent L-lactate uptake. The results are consistent with the existence of an electroneutral Na+/L-lactate co-transport system in the brush border of rabbit small intestine.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002414:Cations, Monovalent / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D066298:In Vitro Techniques; D007583:Jejunum / Q000378:metabolism* / Q000648:ultrastructure; D007773:Lactates / Q000378:metabolism*; D008564:Membrane Potentials; D008854:Microscopy, Electron; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009994:Osmolar Concentration; D011817:Rabbits; D012964:Sodium / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000494:pharmacology
D002414:Cations, Monovalent; D007773:Lactates; D013438:Sulfhydryl Compounds; D012964:Sodium; C023884:thiolactic acid