[Determination of somatotropin and prolactin by dodecyl sulfate polyacrylamide gel electrophoresis].
Definite separation of somatotropin and prolactin using sodium dodecylsulphate polyacrylamide gel electrophoresis takes place in continuous systems with pH 7.0-7.2 containing acetate or chloride monovalet anions; substitution of the ions by polyvalent phosphate or borate anions interfered with the separation. The hormones precipitated by trichloracetic acid may be separated and quantitatively determined after dissolving in the weak alkaline solutions. The hormones are distinctly separated from serum proteins and may be quantitatively determined in media containing up to 15% of serum.
D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005260:Female; D013006:Growth Hormone / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010903:Pituitary Gland, Anterior / Q000032:analysis; D011388:Prolactin / Q000032:analysis*; D051381:Rats; D012967:Sodium Dodecyl Sulfate
D007202:Indicators and Reagents; D012967:Sodium Dodecyl Sulfate; D011388:Prolactin; D013006:Growth Hormone
Opredelenie somatotropina i prolaktina dodetsilsul'fat-élektroforezom v poliakrilamidnom gele.