Effect of sodium chloride on the activity and production of staphylococcal exonuclease.
Extracellular nuclease activity in Staphylococcus aureus was enhanced about fourfold by 1% (w/v) NaCl, KCl, CsCl or LiCl. The pH and concentration of Ca2+ for optimum activity varied with the NaCl concentration; with an increased NaCl concentration, a higher Ca2+ concentration and a lower pH were required. Vmax, but not Km, varied with the concentration of NaCl. The addition of 3% (w/v) NaCl to growing cultures of S. aureus increased nuclease production fivefold.
D002118:Calcium / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D008836:Micrococcal Nuclease / Q000096:biosynthesis*; D012965:Sodium Chloride / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000201:enzymology*
D012965:Sodium Chloride; D008836:Micrococcal Nuclease; D002118:Calcium