title: DNA-content in isolated nuclei of postembryonic stages of progeny from normal and irradiated males of Tetranychus urticae Koch (Acari, Tetranychidae).
abstract: The 1C DNA-content of isolated nuclei of postembryonic stages of Tetranychus urticae stained with the classic and a modified Schiff's reagent was cytophotometrically estimated as 0.1 pg, a low value in animals. For many tissues of this arrhenotokous species the ploidy ratio between males and females is 1:2, indicating the absence of sex-related differences in ploidy.--In addition, DNA measurements were performed to evaluate irradiation-experiments, starting with X-irradiation of mature sperm in males with doses known from previous work to induce chromosomal fragments that are subject to loss and missegregation in the embryonic mitotic stages of the female progeny despite the presumed holokinetic nature of the chromosomes. The DNA-content of the nuclei of the surviving postembryonic preadult stages did not indicate the occurrence of nuclei with in-between male/female values, ruling out loss and missegregation of fragments as important factors in postembryonic lethality. Abnormally low DNA-values in some adult females could be attributed to development of embryos before oviposition caused by radiation-induced effects.
mesh_terms: D000053:Acari / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D002467:Cell Nucleus / Q000528:radiation effects; D002875:Chromosomes / Q000648:ultrastructure*; D003433:Crosses, Genetic; D004247:DNA / Q000378:metabolism* / Q000528:radiation effects; D005260:Female; D008297:Male; D012737:Sex Factors; D013094:Spermatozoa / Q000378:metabolism / Q000528:radiation effects*
chemical_list: D004247:DNA