Isolated hamster brown fat cells: a bioassay for toxicity tests at the cellular level. Effect of cigarette smoke condensates from high and low tar yield cigarettes.
A test system for the detection of substances with cell-irritating or cell-damaging effects using isolated brown fat cells is presented and the effects of cigarette smoke condensates (CSC) from high and low tar yield cigarettes on this system examined. The condensates inhibited noradrenaline stimulated respiration in a dose-dependent manner. Ethanol solutions of the condensates had a stronger inhibitory effect than DMSO solutions. The CSC strongly inhibited the mitochondrial function but other cellular functions were also impaired.
D002001:Adipose Tissue, Brown / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D006224:Cricetinae; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D066298:In Vitro Techniques; D008647:Mesocricetus; D009538:Nicotine / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D012907:Smoking; D012996:Solutions
D012996:Solutions; D009538:Nicotine; D009638:Norepinephrine