[Isolation and characteristics of the messenger RNA of an interferon repressor].
The methods of induction, isolation and testing of the functional activity of the informative RNA of the interferon production repressor were developed. The repressor informative RNA was found in the cells an hour after the repeated induction of interferon. The minimal time of its translation, i.e. 25--30 minutes was determined. It was shown that the iRNA of the repressor was heterogenous and its major peaks had the sedimentation constants of 10S, 22S and 32S. Poly(A)-containing RNA of the repressor was isolated from the summation compound. It had a high specific activity.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D007369:Interferon Inducers / Q000494:pharmacology; D007372:Interferons / Q000037:antagonists & inhibitors*; D008722:Methods; D011061:Poly A / Q000302:isolation & purification; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification*; D012097:Repressor Proteins / Q000032:analysis*; D013997:Time Factors; D014157:Transcription Factors / Q000032:analysis*
D007369:Interferon Inducers; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D011061:Poly A; D007372:Interferons
Vydelenie i kharakteristika informatsionnoĭ RNK repressora interferona.