Alterations of lipid and protein composition in myelin of acute experimental allergic neuritis.
Lipid and protein components of the myelin isolated from the spinal roots of rabbits at the acute stage of experimental allergic neuritis (EAN) and of control rabbits were analyzed. The results obtained from the EAN myelin in comparison with the control myelin were as follows: (1) The content of cholesterol increased and that of cerebroside significantly decreased, while the content of plasmalogen and phospholipid was not changed. The level of cholesterol ester was negligible both in EAN and control myelin. (2) The sum of saturated fatty acids increased while the sum of unsaturated fatty acids decreased. (3) The decrease of the basic proteins P1 and P2 was more noticeable than that of the PO + Y protein. Concomitant to these observations, with 1,6-diphenyl 1,3,5-hexatriene as a fluorescent probe, the microviscosity of the isolated myelin was measured. The results indicated that myelin obtained from EAN was less fluid. Diminished myelin fluidity in EAN was consistent with the results of the lipid and fatty acid analyses.
D000208:Acute Disease; D000818:Animals; D002784:Cholesterol / Q000032:analysis; D005260:Female; D006967:Hypersensitivity / Q000150:complications / Q000378:metabolism*; D008055:Lipids / Q000032:analysis*; D009185:Myelin Proteins / Q000032:analysis* / Q000276:immunology; D009186:Myelin Sheath / Q000032:analysis*; D009443:Neuritis / Q000209:etiology / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D014783:Viscosity
D008055:Lipids; D009185:Myelin Proteins; D010743:Phospholipids; D002784:Cholesterol