Crystallization of a fluorescent derivative of glyceraldehyde-3-phosphate dehydrogenase.
By phosphorus analysis of the fluorescent derivative produced by ultraviolet irradiation of carboxymethylated glyceraldehyde-3-phosphate dehydrogenase in the presence of NAD at saturation levels, it has been shown that the photochemical reaction leading to the formation of the new fluorophore is also a 'half-of-the-sites' reaction. Both the carboxymethylated enzyme and the irradiated enzyme carrying the new fluorophore have been crystallized.
D000818:Animals; D003460:Crystallization; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis / Q000528:radiation effects*; D009132:Muscles / Q000201:enzymology; D009243:NAD / Q000032:analysis; D010777:Photochemistry; D011817:Rabbits; D014466:Ultraviolet Rays
D009243:NAD; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases