Distribution of active gene sequences: a subset associated with tightly bound chromosomal proteins.
The distribution of active polyadenylate-messenger RNA sequences in fractionated chicken liver chromatin was examined. A portion of these active gene sequences is concentrated in a DNA fraction retained by tightly bound nonhistone chromosomal proteins, while the nonretained DNA fraction is substantially depleted of a portion of these sequences. These findings suggest that the tightly bound nonhistones are physically associated with a subset of active gene sequences.
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D005796:Genes; D008099:Liver / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D012965:Sodium Chloride
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D012333:RNA, Messenger; D012965:Sodium Chloride; D004247:DNA