A rapid method for assessment of a macrophage chemotactant produced by SaD2 fibrosarcoma cells in vitro.
A new rapid method for assessing murine macrophage chemotaxis was developed using peritoneal exudate cells labeled with [5,6-3H]uridine in modified Boyden chambers with double polycarbonate filters. The method gave positive results with endotoxin-treated mouse serum and with serum-free culture supernatant of the DBA/2 SaD2 fibrosarcoma. These results correlated with results obtained with conventional microscopic assessment of chemotaxis. Using this method, the SaD2 supernatant was shown to be chemotactic rather than chemokinetic.
D000818:Animals; D002630:Chemotactic Factors / Q000032:analysis*; D005354:Fibrosarcoma / Q000276:immunology* / Q000378:metabolism; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology*; D011854:Radiochemistry / Q000379:methods*
D002630:Chemotactic Factors