The stoichiometry of ion fluxes during Sr2+-induced oscillations in mitochondria.
A quantitative study of H+, K+, Sr2+ and succinate fluxes in Sr2+-induced oscillatory state of rat liver mitochondria is presented. It was shown that oscillation of succinate content in mitochondria occurs synchronously with oscillations of the cation fluxes. Total charge transferred across the membrane by the registered cations and the succinate-anion is equal to zero. Passive H+-influx has been calculated at all stages of the oscillatory cycle. The conclusion is made that electroneutral 2 H+/Sr2+ exchange is periodically induced in mitochondria. A value of (2 +/- 0.2) . 10(-7) mol Sr2+/min per mg protein has been determined for Sr2+ by this type of exchange.
D000818:Animals; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D013324:Strontium / Q000494:pharmacology*
D011188:Potassium; D013324:Strontium