Formation of apparent presynaptic elements in response to poly-basic compounds.
Neuronal cell processes in dispersed cell culture grow out onto Sepharose beads coated with poly-basic proteins. Apparent presynaptic elements are formed by these neuronal processes with the bead serving as a postsynaptic element. It is suggested that electrostatic binding between poly-acidic groups on the presynaptic element and poly-basic troups on the postsynaptic element is one of the initial steps in the formation of a synaptic contact in culture.
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology*; D006863:Hydrogen-Ion Concentration; D009474:Neurons / Q000187:drug effects; D011506:Proteins / Q000494:pharmacology; D051381:Rats; D013569:Synapses / Q000187:drug effects*
D011506:Proteins