Sensitive gas chromatographic assay for the quantitation of bretylium in plasma, urine and myocardial tissue.
A sensitive analytical method has been developed for the quantitation of bretylium in plasma, urine and myocardial tissue. Bretylium and the internal standard, UM-360 (o-iodobenzyltrimethylammonium), are extracted and isolated as the iodide salts. Sodium benzenethiolate is added and the mixture heated to 100 degrees for one hour. This results in the formation of 2-bromobenzyl phenyl thioether and 2-iodobenzyl phenyl thioether, which can be separated and quantitated by gas chromatography. Good reliability and reproducibility can be obtained using electron-capture detection with quantities of bretylium as small as 1 ng.
D000818:Animals; D001949:Bretylium Compounds / Q000097:blood* / Q000494:pharmacology / Q000652:urine; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008297:Male; D009206:Myocardium / Q000032:analysis
D001949:Bretylium Compounds