[Determination of serum iron; a comparison of two methods: Teepol/dithionite/bathophenanthroline versus guanidine/ascorbic acid/Ferrozine (author's transl)].
Two spectrophotometric methods for the assay of serum iron without deproteinisation are compared: 1. Liberation of iron by a detergent (Teepol SHELL), reduction by dithionite, chelation by bathopehanthroline disulfonate; 2. Liberation of iron by 6 mol/l guanidine, reduction by ascorbic acid, chelation by 3-(2-pyridyl)-5,6-bis-(4-phenyl sulfonic acid)-1,2,4-triaxine (Ferrozine).
D001205:Ascorbic Acid; D002614:Chelating Agents; D003902:Detergents; D004227:Dithionite; D005233:Fatty Alcohols; D005297:Ferrozine; D006146:Guanidines; D006801:Humans; D007501:Iron / Q000097:blood*; D010084:Oxidation-Reduction; D010618:Phenanthrolines; D013053:Spectrophotometry / Q000379:methods
D002614:Chelating Agents; D003902:Detergents; D005233:Fatty Alcohols; D006146:Guanidines; D010618:Phenanthrolines; D004227:Dithionite; D005297:Ferrozine; C017049:bathophenanthroline disulfonic acid; C003381:teepol; D007501:Iron; D001205:Ascorbic Acid
Bestimmung von Eisen im Serum: Methodenvergleich Teepol/Dithionit/Bathophenanthrolin gegen Guanidin/Ascorbinsäure/Ferrozin.