Protein organization in Newcastle disease virus as revealed by perturbant treatment.
Treatment of Newcastle disease virus with lithium diiodosalicylate differentially elutes the internally disposed proteins, M and NP, showing that these proteins are extrinsic, i.e., not associated with the lipid hydrophobic core. This selective elution requires disruption of the viral envelope, a process that is maximal at low temperature and influenced by the lipid composition of the virus envelope.
D004591:Electrophoresis, Polyacrylamide Gel; D007463:Iodobenzoates; D008094:Lithium / Q000494:pharmacology; D009522:Newcastle disease virus / Q000032:analysis* / Q000648:ultrastructure; D012459:Salicylates / Q000494:pharmacology; D013696:Temperature; D014764:Viral Proteins / Q000032:analysis*
D007463:Iodobenzoates; D012459:Salicylates; D014764:Viral Proteins; C020891:3,5-diiodosalicylic acid; D008094:Lithium