Uptake of gentamicin by separated, viable renal tubules from rabbits.
The proximal renal tubules have a marked affinity for gentamicin; they also are the major site of nephrotoxicity caused by this drug. The uptake of radiolabeled gentamicin in separated, viable renal tubules prepared by enzymatic digestion of rabbit kidneys was studied. The preparations showed rapid initial uptake of gentamicin followed by continued slower uptake. Accumulation was not affected by pH, but was significantly inhibited by ouabain, dinitrophenol, anoxia, and hypothermia in the absence of evident cellular damage. At gentamicin concentrations of greater than 50 microgram/ml in the medium, there was competition for drug uptake. Gentamicin efflux in tubules that were taken from a medium containing antibiotic and placed into antibiotic-free fluid was slow and incomplete. From these data it appears that gentamicin uptake by separated renal tubules occurs by a process that requires metabolic energy; thereafter, the drug resides in a poorly exchangeable cellular pool.
D000617:Aminoglycosides / Q000378:metabolism; D000818:Animals; D004140:Dinitrophenols / Q000378:metabolism; D004573:Electrolytes / Q000032:analysis / Q000378:metabolism; D005839:Gentamicins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010042:Ouabain / Q000378:metabolism; D011817:Rabbits; D014527:Uric Acid / Q000378:metabolism; D010130:p-Aminohippuric Acid / Q000378:metabolism
D000617:Aminoglycosides; D004140:Dinitrophenols; D004573:Electrolytes; D005839:Gentamicins; D014527:Uric Acid; D010042:Ouabain; D010130:p-Aminohippuric Acid