[Study on histological and histochemical appearance of fetal mouse tibiae cultured in vitro in comparison with freshly excised tibiae (author's transl)].
The histological and histochemical appearance of murine fetal tibiae was studied in order to furnish the morphological background for biochemical investigations on in vitro cultures of these organs, used as an experimental model for the study of drug influences on metabolic processes of mesenchymal tissues. Freshly excised tibiae until the 14th day of gestation showed solely cartilagenous structures; first signs of calcification were found after 15 days of gestation and ossification of the diaphysis proceeds until the 17th day. A comparison of tibiae explants excised on the 17th day of gestation and cultured for 6 days in vitro with freshly excised tibiae of the same age (17 days) indicates a continuous growth of the explants in vitro without any signs of degenerative processes which could be due to the culture conditions.
D000818:Animals; D005260:Female; D005865:Gestational Age; D006651:Histocytochemistry; D051379:Mice; D009924:Organ Culture Techniques; D011247:Pregnancy; D013977:Tibia / Q000032:analysis / Q000196:embryology*
Vergleichende Untersuchungen zum histologischen und histochemischen Bild in vitro kultivierter und frisch exzidierter fetaler Mäuse-Tibiae.