Chromatography tubes: a novel RIA technique.
We describe a novel radioimmunoassay in which separation of free and antibody-bound antigen is achieved by passive means. The separation is carried out by a modified form of inverted dry column chromatography on a column, or 'chromatography tube', consisting of a small polystyrene tube packed with a dry, insoluble, hydrophilic resin. Using the radioimmunoassay or digoxin as an example, an exceptionally simple procedure is described with sensitivity, accuracy and precision suitable for clinical purposes. Advantages of the chromatography tube method are discussed.
D000042:Absorption; D000818:Animals; D000941:Antigens; D002845:Chromatography / Q000295:instrumentation*; D003429:Cross Reactions; D003432:Cross-Linking Reagents / Q000494:pharmacology; D006801:Humans; D007457:Iodine Radioisotopes; D011142:Polyvinyl Alcohol / Q000494:pharmacology; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*
D000941:Antigens; D003432:Cross-Linking Reagents; D007457:Iodine Radioisotopes; D011142:Polyvinyl Alcohol