Enhancement of epoxide hydratase activity in rat lung, kidney and liver by dietary antioxidants.
Enhancement of pulmonary epoxide hydratase activity in the rat was obtained by feeding a diet which contained 0.5% ethoxyquin (EQ) or 3,5-di-tert-butyl-4-hydroxytoluene (BHT). The enhancement was less marked (1.6-fold) than in the liver, where a 2-4-fold enhancement of enzyme activity was found after feeding 0.5% antioxidant. A minimal enhancing concentration of 0.1% EQ or BHT in food was established for hepatic epoxide hydratase. In kidney microsomes, elevation of enzyme activity was obtained with 0.5% EQ (1.8-fold), but not with 0.5% BHT. No concomitant increase of cytochrome P-450 content and of aryl hydrocarbon hydroxylase activity was found in the tissues studied.
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D004851:Epoxide Hydrolases / Q000378:metabolism*; D005015:Ethoxyquin / Q000494:pharmacology; D005520:Food Preservatives / Q000494:pharmacology*; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008168:Lung / Q000201:enzymology*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D051381:Rats
D000975:Antioxidants; D005520:Food Preservatives; D002084:Butylated Hydroxytoluene; D005015:Ethoxyquin; D004851:Epoxide Hydrolases