The isolation of the gamma subunit of fetal hemoglocin (HbF) and its use in a radioimmunoassay for HbF.
A method is described for the purification, from fetal hemoglobin (HbF), of the fetal specific globin chain (gamma chain) in its native state. In the absence of alpha chain (the globin chain common to all adult human hemoglobins) gamma chain, when used as an immunogen, is able to express its unique antigenicity. Here, a specific, high titer antiserum raised against gamma chain has been used to establish a sensitive radioimmunoassay for HbF. This approach may be applicable to the measurement of other normal and abnormal hemoglobins.
D000042:Absorption; D000818:Animals; D000918:Antibody Specificity; D001667:Binding, Competitive; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005319:Fetal Hemoglobin / Q000276:immunology / Q000302:isolation & purification*; D005914:Globins / Q000302:isolation & purification; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D008297:Male; D011247:Pregnancy; D011817:Rabbits; D011863:Radioimmunoassay
D007106:Immune Sera; D005914:Globins; D005319:Fetal Hemoglobin