Isolation and characterization of three lutropin isohormones from the pituitary gland of the ostrich (Struthio camelus).
Avian lutropin has been isolated from pituitary glands of the ostrich (Struthio camelus) in three homogeneous forms (designated isohormones). The different homogeneous forms of ostrich lutropin were characterized physically and chemically in terms of molecular weight, electrophoretic mobility, isoelectric points, amino acid and carbohydrate composition. From these characteristics it was evident that the isohormones are very similar. The differences between these isohormones can be attributed to differences in carbohydrate composition, especially sialic acid.
D000818:Animals; D001681:Biological Assay; D001717:Birds; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005643:Fucose / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D007525:Isoelectric Focusing; D007986:Luteinizing Hormone / Q000031:analogs & derivatives / Q000302:isolation & purification*; D008970:Molecular Weight; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D012794:Sialic Acids / Q000032:analysis
D002241:Carbohydrates; D006595:Hexosamines; D006601:Hexoses; D012794:Sialic Acids; D005643:Fucose; D007986:Luteinizing Hormone