Perfusion of isolated human spleens.
Twelve normal human spleens were perfused in vitro for 17 h at normothermia. The histological structure was well preserved. Splenic imprints showed a normal cellular pattern, mitoses, and labelled lymphoid cells after 3H-thymidine labelling. Metabolic parameters were comparable to in vivo data of human spleens. The perfusion system can preserve the structure and function of human spleens in vitro.
D006801:Humans; D008214:Lymphocytes; D008875:Middle Aged; D008938:Mitosis; D010477:Perfusion; D013154:Spleen / Q000473:pathology*; D013696:Temperature; D013997:Time Factors