Phytohemagglutinin-stimulated normal human peripheral blood lymphocytes in folate-depleted medium: an in vitro model for megaloblastic hemopoiesis.
Normal human peripheral blood lymphocytes cultured with phytohemagglutinin in folate-free RPMI 1640 medium supplemented with normal human serum exhibit megaloblastic maturation. These changes are accompanied by a decrease in intracellular folate content, and when compared to folate-replete cells, reveal increased 3H-thymidine incorporation into DNA together with a decreased inhibitory effect of deoxyuridine on 3H-thymidine incorporation. This in vitro system of cellular folate deficiency is a convenient model for analysis of biochemical events accompanying megaloblastic maturation.
D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000378:metabolism; D004913:Erythrocytes, Abnormal; D005494:Folic Acid Deficiency / Q000097:blood*; D006410:Hematopoiesis; D006801:Humans; D008213:Lymphocyte Activation; D008534:Megaloblasts; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D003470:Culture Media; D010835:Phytohemagglutinins; D004247:DNA; D013936:Thymidine; D014529:Uridine