Identification of harman in the rat arcuate nucleus.
This communication describes the presence of 1-methyl-beta-carboline (harman) in the hypophysiotropic area of the hypothalamus which incorporates the arcuate nucleus. Diethyl ether extracts of borate-buffered arcuate homogenates were subjected to silica column chromatography and thin-layer chromatography (TLC). Identification of the beta-carboline was accomplished by use of fluorescent spectrometry, gas chromatography (GC), mass spectrometry (MS) and combined gas chromatography-mass spectrometry (GC-MS).
D000470:Alkaloids / Q000378:metabolism*; D000818:Animals; D002243:Carbolines / Q000378:metabolism; D002421:Caudate Nucleus / Q000378:metabolism; D002849:Chromatography, Gas; D006247:Harmine / Q000031:analogs & derivatives / Q000378:metabolism*; D008297:Male; D008526:Medulla Oblongata / Q000378:metabolism*; D008636:Mesencephalon / Q000378:metabolism; D051381:Rats; D013050:Spectrometry, Fluorescence
D000470:Alkaloids; D002243:Carbolines; D006247:Harmine; C005010:harman