3H-glucosamine and 3H-fucose incorporation by murine peritoneal macrophages "non-divided" during differentiation in vitro.
Normal murine peritoneal macrophages were maintained in culture for 24 hours and 6 days. These cells continuously incorporated 3H-glucosamine and 3H-fucose in their membrane components. For both precursors culture ages, these components may be considered to undergo turnover according to a given rhythm with a 24 hours doubling time. Treatment with neuraminidase did not modify the doubling time, although a slight increase of incorporation of radioactivity was observed. Variations of 3H-fucose and 3H-glucosamine incorporations were observed. Variations of 3H-fucose and 3H-glucosamine incorporations were observed between 24 hours old and 6 days old cultures: 6 days old cultures incorporated more glucosamine than 24 hours old cultures whereas no difference in fucose incorporation was observed between the two cultures.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D005643:Fucose / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism*; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009439:Neuraminidase / Q000378:metabolism; D012795:Sialoglycoproteins / Q000378:metabolism*
D012795:Sialoglycoproteins; D005643:Fucose; D009439:Neuraminidase; D005944:Glucosamine