Further characterization of IgG receptors from human placenta.
A Cell membrane fraction from term human placenta was prepared by homogenization and ultracentrifugation. The fraction was found to bind both human IgG and human serum albumin. Maximal specific binding occurred at pH 5.2, and the amount of binding was dependent upon incubation time, temperature, buffer, and ionic strength. The binding of human serum albumin was inhibited by preincubation with H-IgG but the reverse did not happen.
D001667:Binding, Competitive; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D011971:Receptors, Immunologic / Q000302:isolation & purification*; D012709:Serum Albumin / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D007074:Immunoglobulin G; D011971:Receptors, Immunologic; D012709:Serum Albumin