title: Genetic mapping of bovine mitochondrial DNA from a single animal.
abstract: Using a physical map of bovine mitochondrial DNA derived from the liver of a single Holstein cow, we have determined the location of the genes specifying the large and small ribosomal RNAs by hybridization analysis and electron microscopic observations of R-loop forms. Also, the position of the origin of DNA replication (D-loop) has been located by electron microscopy. Additionally, the direction of D-loop expansion and the polarity of the large and small ribosomal RNA genes were determined.
mesh_terms: D000818:Animals; D002417:Cattle / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D005796:Genes; D008040:Genetic Linkage; D008099:Liver / Q000378:metabolism; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D012335:RNA, Ribosomal