Structure and function of platelet glycocalicin.
Present knowledge of the structure and function of platelet glycocalicin is reviewed. Glycocalicin (M 150,000) is a glycoprotein component of the outer surface of intact platelets which is released in soluble form following platelet homogenization. Glycocalicin has been purified and shown to inhibit platelet aggregation induced by thrombin or by ristocetin. Thrombin binding activity is associated with the peptide "tail" of the molecule (Mr 45,000), the macroglycopeptide portion (Mr 120,000) being without effect. Glycocalicin and membrane-bound glycoprotein I have been shown to be functionally and immunologically identical. Studies with platelets modified by chymotrypsin, and with platelets from patients with Bernard-Soulier disease and an ill-defined bleeding abnormality show that the amount of thrombin bound is proportional to the total amount of glycocalicin and glycoprotein I present. These results support the concept of a single class of binding site for thrombin in platelets.
D000818:Animals; D001667:Binding, Competitive; D001791:Blood Platelet Disorders / Q000097:blood; D001792:Blood Platelets / Q000502:physiology*; D002241:Carbohydrates; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006020:Glycopeptides / Q000494:pharmacology; D006023:Glycoproteins / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D008565:Membrane Proteins / Q000276:immunology / Q000502:physiology*; D008970:Molecular Weight; D010974:Platelet Aggregation; D019038:Platelet Glycoprotein GPIb-IX Complex; D013917:Thrombin / Q000494:pharmacology
D002241:Carbohydrates; D006020:Glycopeptides; D006023:Glycoproteins; D007106:Immune Sera; D008565:Membrane Proteins; D019038:Platelet Glycoprotein GPIb-IX Complex; C023592:glycocalicin; D013917:Thrombin