title: Changes in protein-bound oligomannosyl type glycans during Semliki Forest virus maturation.
abstract: Labelled oligomannosyl type glycans of E2 protein of Semliki Forest virus were liberated by treatment with endo-beta-N-acetylglucosaminidase H, as well as by hydrazinolysis and analysed by paper chromatography. The protein-bound virus oligosaccharides were a mixture of Man7GlcNAc2, Man6GlcNAc2 and Man5GlcNAc2, and Man8GlcNAc2 also appeared to be present. P62 protein, the direct precursor of E2, was labelled by supplying infected BHK cells with 2-3H-mannose for 5 min. The oligomannosyl glycans of the P62 proteins formed were about two monosaccharide units larger than those of the mature virus E2 proteins.
mesh_terms: D002854:Chromatography, Paper; D006023:Glycoproteins / Q000032:analysis*; D008358:Mannose; D009844:Oligosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis*; D012672:Semliki forest virus / Q000032:analysis / Q000254:growth & development*; D014764:Viral Proteins / Q000032:analysis*
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D011134:Polysaccharides; D014764:Viral Proteins; D008358:Mannose