Determination of clonidine in human plasma by glass capillary gas chromatography with electron-capture detection.
A gas chromatography assay for clonidine in human plasma has been developed. The buffered serum is extracted on silica columns, alkylated with pentafluorobenzyl bromide, cleaned up-by extractions and analysed by glass capillary gas chromatography with falling-glass-needle injection and electron-capture detection. A gas chromatographic-mass spectrometric analysis of the derivatives formed and an investigation of the structure dependence of the electron-capture response are presented.
D002197:Capillary Action; D002849:Chromatography, Gas / Q000379:methods; D003000:Clonidine / Q000097:blood*; D006801:Humans
D003000:Clonidine