Sterol requirement for the growth of Treponema hyodysenteriae.
The addition of cholesterol to a liquid medium containing bovine serum albumin (BSA) fraction V or acetone-delipidized BSA fraction V instead of serum stimulated the growth of Treponema hyodysenteriae, a serum-requiring spirochaete associated with swine dysentery. As little as 1.25 micrograms cholesterol ml-1 increased viable counts about 1000-fold. Sitosterol and cholestanol, but not pregnenalone, cholestenone or stigmasteriol, produced a growth response comparable to that of cholesterol. The results suggest that T. hyodysenteriae requires a sterol for growth.
D002784:Cholesterol / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013261:Sterols / Q000378:metabolism; D014208:Treponema / Q000254:growth & development* / Q000378:metabolism
D013261:Sterols; D002784:Cholesterol