Peptide separations on substituted polystyrene resins. Effect of cross-linkage.
The effect of copolymer cross-linkage on the resolution of soluble tryptic peptides of human globin (alpha- and beta-chains) separated in columns containing substituted polystyrene resin classified to 11 +/- 1 micrometer has been examined. With both the cation and anion exchange resins, polymers of lower cross-linkage provided better resolution; inferior resolution was obtained with 12% cross-linked resins. It was also observed that microparticle anion-exchange resins could be used in columns maintained at 55 degrees instead of 35 degrees as used traditionally. Resolution and yield with 20 x 1 cm resin beds were generally as good as or superior to much longer columns of crushed bead resin of the same chemical structure.
D000837:Anion Exchange Resins; D002411:Cation Exchange Resins; D002852:Chromatography, Ion Exchange / Q000379:methods; D005914:Globins; D006801:Humans; D046911:Macromolecular Substances; D010446:Peptide Fragments / Q000302:isolation & purification*; D014357:Trypsin
D000837:Anion Exchange Resins; D002411:Cation Exchange Resins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D005914:Globins; D014357:Trypsin