Identification and measurement of rat eosinophil phospholipase D. Its activity on schistosomula phospholipids.
A sensitive assay, using [14C]lecithin as a substrate, has been developed for the measurement of phospholipase activity in rat peritoneal polymorphonuclear leukocytes. Cell extracts were found to contain a phospholipase D activity and indirect evidence suggested that eosinophils are responsible for the cleavage of lecithin. Intact peritoneal cells were also able to hydrolyze exogenous [14C]lecithin in vitro. When [3H]choline-labeled schistosomula were used as targets in antibody-dependent cytotoxicity experiments, the radioactivity of lecithin decreased more rapidly in a complete cytotoxicity system than in controls, suggesting that hydrolysis of schistosomula phospholipids occurred during the killing process.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002448:Cell Adhesion; D003602:Cytotoxicity, Immunologic; D004804:Eosinophils / Q000201:enzymology*; D006868:Hydrolysis; D008297:Male; D010713:Phosphatidylcholines; D010739:Phospholipase D / Q000378:metabolism* / Q000494:pharmacology; D010740:Phospholipases / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D012550:Schistosoma mansoni / Q000276:immunology*; D010738:Type C Phospholipases / Q000378:metabolism
D010713:Phosphatidylcholines; D010743:Phospholipids; D010740:Phospholipases; D010738:Type C Phospholipases; D010739:Phospholipase D