A simple procedure for the purification of bovine fibrin from clotted blood by the use of detergents.
A rapid and simple procedure for the preparation, directly from clotted whole blood, of bovine fibrin free of cell membrane contaminants is described. The procedure exploits the ability of detergents to solubilize and extract cell membrane proteins from homogenized clot fragments. The non-denaturing detergents, TX-100 and DOC, used in sequence, or the denaturing detergent, SDS, used alone, proved equally effective. The resulting fibrin has the solubility properties and the subunit composition on SDS gels of highly cross-linked fibrin.
D000818:Animals; D001777:Blood Coagulation; D002417:Cattle; D002462:Cell Membrane; D003840:Deoxycholic Acid; D003902:Detergents; D005337:Fibrin / Q000302:isolation & purification*; D046911:Macromolecular Substances; D008722:Methods; D017830:Octoxynol; D011092:Polyethylene Glycols; D011489:Protein Denaturation; D012967:Sodium Dodecyl Sulfate; D012995:Solubility
D003902:Detergents; D046911:Macromolecular Substances; D003840:Deoxycholic Acid; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D005337:Fibrin; D017830:Octoxynol