Adenylate cyclase activity in the uterine cervix of neonatal and immature mice: influence of oestradiol-17 beta.
The influence of long-term oestradiol stimulation on adenylate cyclase activity was investigated in homogenates from uterine cervices of neonatal (3-day-old) and immature (14-day-old) NMRI mice. A significant increase of enzyme activity, measured in the presence of fluoride ions (NaF), after oestradiol treatment was found in preparations from neonatal but not from those of immature female mice; this was irrespective of whether the enzyme activity was related to wet weight, amount of protein or DNA content. The enzyme activity was stimulated in vitro by NaF and 5-guanylylimidodiphosphate while isoprenalin, histamine, prolactin and oestradiol had no effect. These results indicated an age-related difference in the effects of oestradiol in the uterine cervix of the mouse.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000375:Aging; D000818:Animals; D002584:Cervix Uteri / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D051379:Mice; D011388:Prolactin / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism; D013268:Stimulation, Chemical
D011506:Proteins; D006165:Guanylyl Imidodiphosphate; D004958:Estradiol; D006632:Histamine; D011388:Prolactin; D004247:DNA; D000262:Adenylyl Cyclases; D007545:Isoproterenol