The fractionation of plant photoactive pigment-protein complexes I and II.
The pigment-protein complexes enriched with Photosystem I (PPC-I) and Photosystem II (PPC-II) were obtained using sievorptive chromatography on DEAE-Sephadex column. Both types of complexes contain Chlorophyll a, beta-carotene and minor quantities of Chl b. Red absorbance maxima are located at 676 nm and 673 nm for PPC-I and PPC-II, respectively. The degrees of reaction centre enrichment were measured by the method of differential spectrophotometry: PPC-I has one P-700 per 35 bulk Chl a molecules, PPC-II contains one P-680 per 18 bulk Chl a molecules. The yield of PPC-II is 7--10 times lower than that of PPC-I. After one chromatographic procedure the amount of P-680 in PPC-I preparation does not exceed 7% of that of P-700, the amount of P-700 in PPC-II preparation 2% of that of P-680. The product of PPC-II degradation was studied.
D002338:Carotenoids / Q000032:analysis; D002734:Chlorophyll / Q000032:analysis / Q000302:isolation & purification*; D002736:Chloroplasts / Q000032:analysis*; D002848:Chromatography, DEAE-Cellulose; D007700:Kinetics; D008027:Light; D010788:Photosynthesis; D010940:Plant Proteins / Q000032:analysis / Q000302:isolation & purification*; D010944:Plants / Q000032:analysis; D013057:Spectrum Analysis
D010940:Plant Proteins; D002734:Chlorophyll; D002338:Carotenoids; C001785:chlorophyll P 700; C025167:P-680