Interferences appearing in fluorometrically measured liquid-chromatographic profiles of creatine kinase isoenzymes in serum.
We observed nonenzymic peaks when serum isoenzymes of lactate dehydrogenase (EC 1.1.1.27; LD) and creatine kinase (EC 2.7.3.2.; CK) were separated by "high-performance" liquid chromatography and detected by continuously monitoring the column effluent for enzyme activity. Such background peaks were particularly apparent in CK isoenzyme profiles obtained from human sera. We observed two nonenzymic peaks with fluorescence detection, one in the CK-MB region, the other in the CK-BB region. Serum albumin was a major component in the artifactual CK-MB peak, with lipoprotein as a minor component. We present evidence that the material responsible for the other peak fluoresced quite strongly and is mostly pre-albumin.
D001798:Blood Proteins / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D003402:Creatine Kinase / Q000097:blood*; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood; D012709:Serum Albumin / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods
D001798:Blood Proteins; D007527:Isoenzymes; D012709:Serum Albumin; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase