Analysis of life-shortening effects in female BALB/C mice fed 2-acetylaminofluorene.
A total of 4320 female BALB/cStCrlfC3H/Nctr mice were continuously fed 2-acetylaminofluorene (2-AAF) in the diet at various concentrations in order to obtain a quantitative assessment of dose-related life-shortening effects. Statistical procedures which adjust for extraneous deaths were used to analyze the survival data. In addition to the demonstration of a reduced survival time associated with bladder and liver tumors induced by 2-AAF, a further toxic effect was observed at 150 ppm. Since the experiment was replicated six times, variability in the survival times of animals in different replicates was studied. Although all replicates were conducted under specific pathogen free / defined flora (SPF/DF) conditions, a significant change in survival time was demonstrated across replicates.
D015073:2-Acetylaminofluorene / Q000633:toxicity*; D000818:Animals; D004032:Diet; D005260:Female; D008017:Life Expectancy; D008113:Liver Neoplasms / Q000139:chemically induced; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000139:chemically induced; D013997:Time Factors; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced
D015073:2-Acetylaminofluorene