title: Time dependent effects of adriamycin and x-ray therapy on wound healing in the rat.
abstract: Wound healing as measured by wound breaking strength (WBS) was studied in male Fischer rats. Animals were wounded (day 0) and treated with Adriamycin (ADR), x-ray therapy (XRT), or the combination of ADR + XRT. Treatments were either on day -7,0 or +7. Animals were killed on days +14 and +21, and excised wounds were subjected to uniaxial extension and the recording of WBS (grams), hydroxy-proline assay, and collagen fiber diameter measurements. Day 0 treatment yielded WBS averaging 46% (ADR), 41% (XRT), and 26% (ADR + XRT) of control by 21 days. Newly synthesized hydroxyproline (scar collagen) values of day 0 treated animals were 47% (ADR), 66% (XRT), and 39% (ADR + XRT) of control at 21 days. Day -7 treatment yielded similar WBS values. Withholding single agent or combined treatment until day +7 yielded WBS values comparable to control at 14 or 21 days. In this model, day 0 treatment with ADR, XRT, or ADR + XRT impaired WBS most significantly. This was supported by a diminution in newly synthesized hydroxyproline and load extension curve analysis.
mesh_terms: D000818:Animals; D003094:Collagen / Q000032:analysis; D004317:Doxorubicin / Q000008:administration & dosage* / Q000494:pharmacology; D006909:Hydroxyproline / Q000032:analysis; D007275:Injections, Intravenous; D008297:Male; D051381:Rats; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects* / Q000528:radiation effects; D014965:X-Rays
chemical_list: D004317:Doxorubicin; D003094:Collagen; D006909:Hydroxyproline