Induction of Fc epsilon-receptor bearing cells in vitro in human peripheral lymphocytes.
Fc epsilon R-bearing lymphocytes were induced in vitro by the culture of peripheral lymphocytes from ragweed-sensitive patients with antigen E together with, or followed by, human IgE. Neither antigen E alone nor IgE alone induced Fc epsilon R(+) cells. As expected, culture of normal lymphocytes with the antigen and IgE failed  to increase Fc epsilon R(+) cells. However, mixed lymphocyte cultures from two normal individuals responsed to human IgE for the induction of Fc epsilon R(+) cells. In both antigen-stimulated and mixed lymphocyte cultures, a portion of Fc epsilon R(+) cells induced in vitro were T lymphocytes. Evidence was obtained that Fc gamma R(+) cells are involved in the induction of Fc epsilon R(+) cells. Depletion of Fc gamma R(+) cells in the lymphocytes from ragweed-sensitive patients, before culture with antigen E and IgE, abolished the ability of the lymphocytes to respond to IgE for an increase in Fc epsilon R(+) cells.
D004247:DNA / Q000096:biosynthesis; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D011961:Receptors, Fc / Q000276:immunology*; D012397:Rosette Formation
D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc; D007073:Immunoglobulin E; D004247:DNA