31P nuclear magnetic resonance study on perfused brain slices of guinea pig.
31P-NMR measurements of brain slices of guinea pig were successfully carried out for the first time with a new perfusion and spinning technique. The 31P-NMR spectrum showed resonance lines of phosphocreatine, fructose-6-phosphate, ATP, and inorganic phosphate. In the time course of the 31P-NMR spectra, the concentrations of phosphocreatine and fructose-6-phosphate, which were estimated from the initial spectrum, were 4.3 and 1.1 mumol/g of fresh tissue, respectively, and the estimates from the following ones showed that the concentration of phosphocreatine decreased to 2.7 mumol/g of fresh tissue while that of fructose-6-phosphate did not change significantly throughout the measurements.
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002540:Cerebral Cortex; D005636:Fructosephosphates / Q000032:analysis; D006168:Guinea Pigs; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy; D010710:Phosphates / Q000032:analysis; D010725:Phosphocreatine / Q000032:analysis; D010758:Phosphorus
D005636:Fructosephosphates; D010710:Phosphates; D010725:Phosphocreatine; D010758:Phosphorus; D000255:Adenosine Triphosphate