A large-scale radiometric micro-quantitative complement fixation test for serum antibody titration.
A micro-quantitative complement fixation (CF) procedure based on 51Cr release is described. The method employs 50% hemolysis as end point and the alternation equation to calculate the amount of complement involved in the hemolytic reaction. Compared to the conventional CF tests, the radiometric procedure described here is very precise and consistently reproducible. Also, since only 3 4-fold dilutions of sera are used for the titration of antibodies over a wide range of concentrations, the test is very concise and is economical to perform. Its format is amenable to automation and computerization. This radiometric CF procedure is thus most useful for large-scale immunological research and epidemiological surveillance studies.
D000818:Animals; D000906:Antibodies; D001322:Autoanalysis; D002860:Chromium Radioisotopes; D003168:Complement Fixation Tests / Q000379:methods*; D003165:Complement System Proteins / Q000276:immunology; D003201:Computers; D006168:Guinea Pigs; D006460:Hemolysin Proteins; D006801:Humans; D008433:Mathematics; D011874:Radiometry; D012756:Sheep
D000906:Antibodies; D002860:Chromium Radioisotopes; D006460:Hemolysin Proteins; D003165:Complement System Proteins