title: Marine biopolymers with cell specificity. II. Purification and characterization of agglutinins from mucus of windowpane flounder Lophopsetta maculata.
abstract: The windowpane flounder, Lophopsetta maculata, was found to have proteins in the body mucus which agglutinate mouse leukemia cells, L5784Y but not L1210. They also agglutinate rabbit and mouse erythrocytes, a marine yeast and a bacterium, and have weak activity against mouse sarcoma 180 cells, human B, guinea pig, and horse erythrocytes. The hemagglutinating activity was not affected by the treatment with 2-mercaptoethanol, trypsin, or pronase, but was inhibited by a high concentration of N-acetylneuraminic acid. The major active component was purified and found to be a protein having a molecular weight of 68 000 which dissociates into subunits of equal size (16 000). Isoelectrofocusing gave two sharp bands, close together, at pI 4.7 +/- 0.1. The protein contains high amounts of aspartic acid, glutamic acid and glycine, and very little histidine and half-cystine.
mesh_terms: D000373:Agglutinins / Q000302:isolation & purification*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001419:Bacteria / Q000276:immunology; D005399:Fishes / Q000276:immunology*; D006168:Guinea Pigs; D006388:Hemagglutinins / Q000302:isolation & purification; D006801:Humans; D066298:In Vitro Techniques; D007526:Isoelectric Point; D007942:Leukemia, Experimental / Q000276:immunology; D051379:Mice; D008970:Molecular Weight; D009093:Mucus / Q000276:immunology*; D011817:Rabbits; D012794:Sialic Acids / Q000494:pharmacology; D015003:Yeasts / Q000276:immunology
chemical_list: D000373:Agglutinins; D000596:Amino Acids; D006388:Hemagglutinins; D012794:Sialic Acids