title: Prostaglandin E2 catabolism in the spinal nerve roots of the cat.
abstract: Catabolism of prostaglandin (PG) E2 was studied in homogenates of spinal cord and spinal nerve roots of the cat. Spinal roots enzymatically converted PGE2 to a product (metabolite I) with the chromatographic mobility of 15-keto-PGE2. Little metabolic degradation occurred in the spinal cord; however, incubation of PGE2 with combined spinal cord and nerve root tissue yielded a second metabolite (metabolite II) in addition to metabolite I. Metabolite II was identified as 15-keto-13, 14-dihydro-PGE2. These results prove that spinal nerve roots, unlike the spinal cord, contain 15-hydroxyprostaglandin dehydrogenase (15-PGDH) which is the major and rate-limiting enzyme in the inactivation of prostaglandins. The location and functional significance of 15-PGDH in peripheral nerves remain to be elucidated.
mesh_terms: D000818:Animals; D002415:Cats / Q000378:metabolism*; D005260:Female; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D011458:Prostaglandins E / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism; D013126:Spinal Nerve Roots / Q000378:metabolism*
chemical_list: D011458:Prostaglandins E; D006911:Hydroxyprostaglandin Dehydrogenases