Inhibition of microsome-mediated binding of aflatoxin B1 to DNA by glutathione S-transferase.
Hamster liver microsome-mediated [14C]aflatoxin B1 binding to DNA is inhibited by 105,000 X g liver supernatant fraction. Absolute requirement of glutathione for the inhibitory activity of the dialyzed supernatant and irreversible loss of the inhibitory activity by the heat-treated supernatant indicate involvement of glutathione S-transferase. Concomitant with inhibition of aflatoxin B1-DNA binding, the formation of an aflatoxin B1-glutathione conjugate is indicated.
D000348:Aflatoxins / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D006224:Cricetinae; D004247:DNA / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus; D008862:Microsomes, Liver / Q000201:enzymology*
D000348:Aflatoxins; D004247:DNA; D005982:Glutathione Transferase