Characterization of retinoblastoma immune complexes.
Immune complexes from retinoblastoma sera were characterized with molecular sieve chromatography, affinity chromatography, and polyacrylamide gel electrophoresis (PAGE). Retinoblastoma patients' sera had two well-defined peaks of immune complex activity after molecular sieve chromatography. These protein fractions had a molecular weight of approximately 1.6 x 10(5) and 2.0 x 10(6) daltons. Affinity chromatography with Sepharose 4B-protein A and analytical PAGE demonstrated that IgG was the predominant immunoglobulin in these immune compelxes. Immune complexes also had affinity for Sepharose-concanavalin A, indicating the glycoprotein nature of the antigen component.
D000936:Antigen-Antibody Complex; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005134:Eye Neoplasms / Q000276:immunology*; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D012175:Retinoblastoma / Q000276:immunology*
D000936:Antigen-Antibody Complex; D006023:Glycoproteins; D007074:Immunoglobulin G