Reduction of nitro-BT by some components of the chloroform-methanol extract of the grey matter of the rat brain after reserpine.
Investigations have been performed with the chloroform-methanol extract obtained from the grey matter of the rat brain, to find out if some of the fractions interfere with catecholamines in the reduction of Nitro-BT. The experiments were conducted on white Wistar rats, which were reserpinized with a dose of 10 mg/kg of body weight through 24 hrs. The fractions which were prepared from the chloroform-methanol extract of the grey matter of the rat brain after reserpinisation were compared with fraction of the control animals. The spectrophotometric measurements of the reduced Nitro-BT to formazan have shown, that one of the fractions, namely the water fraction of the reserpinized rats had the extinction curve significantly decreased as compared with the water fraction of the control animals. This observation favours the view, that there can exist an interference of catecholamines with the substances present in the water fraction.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002725:Chloroform / Q000494:pharmacology; D004336:Drug Antagonism; D005260:Female; D008297:Male; D000432:Methanol / Q000494:pharmacology; D009580:Nitroblue Tetrazolium / Q000032:analysis / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D012110:Reserpine / Q000494:pharmacology; D013053:Spectrophotometry; D013778:Tetrazolium Salts / Q000378:metabolism*; D014020:Tissue Extracts / Q000032:analysis*
D013778:Tetrazolium Salts; D014020:Tissue Extracts; D009580:Nitroblue Tetrazolium; D002725:Chloroform; D012110:Reserpine; D000432:Methanol