Metabolism and effects of progesterone in the human endometrial adenocarcinoma cell line HEC-1.
Human endometrial adenocarcinoma cells (HEC-1 line) were incubated with 14C-progesterone. Four major labeled metabolites, 3 beta-hydroxy 5 alpha-pregnan-20-one, 5 alpha-pregnane-3 beta, 20 alpha-diol, 20 alpha-hydroxy-4-pregnen-3-one and 5 alpha-pregnane-3, 20-dione were separated by thin layer chromatography, further purified by high pressure liquid chromatography, and finally identified by addition of carriers and crystallization to constant specific activity. Among these metabolites, 5 alpha-pregnane-3 beta, 20 alpha-diol seems characteristic of this cell line since its formation from labeled progesterone was not detected in normal endometrium or in 2 specimens of endometrial adenocarcinoma. The growth of HEC cells was unaffected by either progesterone or medroxyprogesterone acetate, a slowly metabolized progestin, at about 10(-6) M levels but was inhibited by about 10(-5) M concentrations of these compounds.
D000230:Adenocarcinoma / Q000378:metabolism*; D002455:Cell Division; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D005260:Female; D006801:Humans; D008525:Medroxyprogesterone / Q000494:pharmacology; D011374:Progesterone / Q000378:metabolism* / Q000494:pharmacology; D014594:Uterine Neoplasms / Q000378:metabolism*
D011374:Progesterone; D008525:Medroxyprogesterone