The radiobiological response of the thyroid. Part II: Response of sheep thyroid cell in vitro to single doses of X rays.
The response of sheep thyroid cells in culture to single doses of X or gamma rays is described. In the absence of cellular proliferation the cells are unusually radioresistant, showing little sign of interphase death at doses up to 9 krad. The follicular morphology characteristic of thyroid cells in vivo is also very radioresistant. Iodide trapping is reduced to 50% of the control value by doses of the order of 2 krad. When proliferation is induced the cells may be assayed for postirradiation survival using a clonogenic endpoint. The survival curves are sigmoid with a Do of 410 rad and a very low extrapolation number.
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D003470:Culture Media; D005720:Gamma Rays; D007454:Iodides / Q000378:metabolism; D011829:Radiation Dosage; D012756:Sheep; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D013972:Thyrotropin; D013997:Time Factors; D014965:X-Rays
D003470:Culture Media; D007454:Iodides; D013972:Thyrotropin