Processing of intervening sequences: a new yeast mutant which fails to excise intervening sequences from precursor tRNAs.
By using conditional loss of suppression an an assay, we have been successful in screening for a yeast mutant which is defective in tRNA processing. The los1-1 mutation causes an accumulation of a subset of precursor tRNAs at the nonpermissive temperature. These pre-tRNAs are like those which accumulate in the yeast mutant ts 136 (rna1) in that they have transcribed intervening sequences. The mutations at los1-1 and rna1 complement and segregate independently of each other. The los1-1 mutation affects the expression of all 8 tyrosine-inserting suppressor loci, but does not seem to affect rRNA or mRNA synthesis.
D001483:Base Sequence; D005796:Genes; D009154:Mutation; D009694:Nucleic Acid Precursors / Q000096:biosynthesis*; D012331:RNA, Fungal / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis*; D013489:Suppression, Genetic; D014158:Transcription, Genetic; D015003:Yeasts / Q000235:genetics* / Q000378:metabolism
D009694:Nucleic Acid Precursors; D012331:RNA, Fungal; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012343:RNA, Transfer