Comparison of intradermal pigeon crop-sac bioassay and double antibody radioimmunoassay for rat prolactin.
On an absolute basis, the intradermal pigeon crop-sac bioassay (PCA) gave results that were 20.5% higher than the radioimmunoassay (RIA) in rat anterior pituitary (AP) preparations. A highly significant correlation (r = 0.87) was obtained between RIA (in microgram) and PCA (in Reece-Turner units) when 58 medium samples obtained by culturing rat APs in vitro were assayed for prolactin (PRL) content.
D000818:Animals; D001681:Biological Assay; D010856:Columbidae; D003426:Crop, Avian / Q000187:drug effects; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D011388:Prolactin / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats
D011388:Prolactin