[Effect of the preliminary incubation of human peripheral blood lymphocytes on the intensity of transformation in phytohemagglutin stimulation].
Preincubation of lymphocytes in leukocytic suspension or those separated on verografin-Ficoll gradient at 37 degrees C leads to enhancement of blast transformation in response to phytohemagglutinin (PHA) stimulation. This effect is reversed by preincubation in the presence of soybean trypsin inhibitor (500 micrograms/ml). The results obtained suggest the participation of neutral proteinases from neutrophils or monocytes in the modification of PHA response.
D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007962:Leukocytes / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology*
D010835:Phytohemagglutinins
Vliianie predvaritel'noĭ inkubatsii limfotsitov perifericheskoĭ krovi cheloveka na intensivnost' transformatsii pri stimuliatsii fitogemaggliutininom.