Hapten-cell conjugates in DNCB contact sensitivity. In vitro stimulation with DNP-conjugates optimally inducing contact sensitivity in vivo.
Until now in vitro stimulation tests have failed to detect DNCB contact sensitivity in guinea-pigs sensitized epicutaneously without the use of adjuvants. In this study DNP-conjugates optimally inducing contact sensitivity in vivo were tested for their capacity to detect DNCB contact sensitivity in vitro in a lymphocyte transformation assay. In vivo contact sensitivity measurements in guinea-pigs which had been immunized with different hapten-cell conjugates, showed that (1) living cells should be used for conjugation with the allergen, (2) macrophages are optimal carriers and (3) syngeneity is not required. Therefore, DNFB-coated peritoneal macrophages (viable when conjugated) were used as an antigen for in vitro stimulation. Using this conjugate, a highly reproducible antigen-specific increase in DNA synthesis could be obtained in lymph node lymphocytes from guinea-pigs that had been sensitized to DNCB by epicutaneous application of the allergen without the use of adjuvants.
D000818:Animals; D000917:Antibody Formation; D002470:Cell Survival; D003877:Dermatitis, Contact / Q000276:immunology*; D004137:Dinitrochlorobenzene / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D006241:Haptens; D007114:Immunization; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D009578:Nitrobenzenes / Q000276:immunology*; D012882:Skin Tests
D004137:Dinitrochlorobenzene; D006241:Haptens; D009578:Nitrobenzenes