title: Biochemical studies on early cell surface antigen induced by vaccinia and cowpox viruses.
abstract: The early cell surface antigen (ECSA) induced by vaccinia or cowpox virus infection was analysed by immunoprecipitation and SDS-polyacrylamide gel electrophoresis (SDS-PAGE), The components with a mol. wt. of about 40 000 (40 K) in vaccinia virus-infected cells and with a mol. wt. of about 43 000 (43 K) in cowpox virus-infected cells were specifically immunoprecipitated with anti-ECSA serum prepared by immunizing rabbits with rabbit kidney cells infected with a conditional lethal mutant. The 40 K and 43 K polypeptides were synthesized even in the presence of cytosine-I-beta-D-arabinofuranosyl-HCl (Ara C) and were detectable even in the cells pulse-labelled between I and 2 h p.i.
mesh_terms: D000818:Animals; D000954:Antigens, Surface; D002478:Cells, Cultured; D011232:Chemical Precipitation; D003561:Cytarabine / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007668:Kidney; D008970:Molecular Weight; D009154:Mutation; D010452:Peptide Biosynthesis; D011817:Rabbits; D014616:Vaccinia virus / Q000187:drug effects / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D003561:Cytarabine