Binding of enzyme--IgG complexes in human serum to Protein-A Sepharose CL-4B.
We describe a simple method for detecting enzyme--immunoglobulin G (IgG) complexes in human serum. Protein-A Sepharose CL-4B binds IgG and therefore also the enzyme--IgG complexes, which can then be separated easily from the serum by centrifugation. We demonstrate this separation in two patients, one with a complex of IgG and creatine kinase (EC 2.7.3.2) BB isoenzyme, the other with an IgG--alkaline phosphatase (EC 3.1.3.1) complex. Both patients had unexplainably high activities of the respective enzymes in their serum. The method we propose should be a useful, simple, routine method of detection in cases where IgG--enzyme complexes are suspected.
D000368:Aged; D000469:Alkaline Phosphatase / Q000097:blood*; D003327:Coronary Disease / Q000097:blood; D003402:Creatine Kinase / Q000097:blood*; D004586:Electrophoresis; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007527:Isoenzymes; D011134:Polysaccharides / Q000378:metabolism*; D011485:Protein Binding; D012685:Sepharose / Q000378:metabolism*; D013205:Staphylococcal Protein A / Q000378:metabolism*
D007074:Immunoglobulin G; D007527:Isoenzymes; D011134:Polysaccharides; D013205:Staphylococcal Protein A; D012685:Sepharose; D003402:Creatine Kinase; D000469:Alkaline Phosphatase