title: Solid-phase synthesis of porcine vasoactive intestinal peptide.
abstract: The 28-residue sequence of porcine vasoactive peptide (VIP) was assembled on a benzhydrylamine resin support, cleaved by HF treatment, and purified by ion-exchange and partition chromatography. In addition to the normal criteria, the homogeneity of the final material, obtained in 16% yield, was assessed by reverse-phase high performance liquid chromatography and the isolation and examination of cyanogen bromide cleavage fragments by the same technique. The purified VIP possessed characteristic inhibitory effects on pentagastrin-induced gastric acid release in the dog. Upon storage, even as the lyophilized powder in vacuo, HPLC revealed the slow formation of a contaminant possibly representing deamidated peptide.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D004285:Dogs; D005750:Gastric Juice / Q000378:metabolism; D005768:Gastrointestinal Hormones / Q000138:chemical synthesis*; D014660:Vasoactive Intestinal Peptide / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D005768:Gastrointestinal Hormones; D014660:Vasoactive Intestinal Peptide