Discharge of intracellular amino acid pools.
Amino acid discharge from a labelled intracellular acid-soluble (acid-extractable) pool occurs as a negative exponential, which is not affected by pool size or concentration of amino acid in the chase medium. Simple negative exponentials were observed over the range 7-37 degrees C, but the slopes were dependent on temperature. Discharge kinetics do not appear to be mirror images of the kinetics of uptake into the acid-extractable pool. The results lend particular support to the efferent limb of the cyclical perfusion model of amino acid utilisation proposed by Wheatley and Inglis (1980).
D000596:Amino Acids / Q000378:metabolism*; D001692:Biological Transport; D002451:Cell Compartmentation; D003593:Cytoplasm / Q000378:metabolism; D004058:Diffusion; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012995:Solubility
D000596:Amino Acids