Transformation of rat tracheal epithelial cells by benzo[a]pyrene and its metabolites.
Benzo[a]pyrene (B[a]P) and its 7,8-dihydrodiol form were metabolically activated to malignantly transform a non-tumorigenic rat tracheal epithelial cell line in culture. The proximal carcinogenic metabolite of B[a]P, diolepoxide I, was more efficient in transforming the epithelial cell line than the intermediate or parent compound, even at a 50- or 100-fold lower concentration, respectively. Inoculations of the transformed cell lines into immunosuppressed isogenic recipients produced differentiated carcinomas similar to those which occur in humans.
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004848:Epithelium / Q000187:drug effects; D005260:Female; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000648:ultrastructure; D051381:Rats; D014132:Trachea / Q000187:drug effects*; D014134:Tracheal Neoplasms / Q000139:chemically induced / Q000648:ultrastructure; D014184:Transplantation, Homologous
D001580:Benzopyrenes