A factor VII concentrate for therapeutic use.
A concentrate of factor VII suitable for therapeutic use has been prepared from human plasma by a method forming part of a comprehensive scheme of large-scale plasma fractionation. Factor VIII was separated as cryoprecipitate and factors II, IX and X were adsorbed on DEAE-cellulose. Most of the factor VII remained in the supernatant. By batch adsorption on DEAE-Sepharose, followed by elution on a chromatographic column, factor VII was concentrated about 25-fold, and purified about 50-fold compared with original plasma, without the need for further dialysis or concentration steps. Data are presented from 10 batches, each from 80-120 kg plasma. Following doses of factor VII to six congenitally deficient patients, the mean rise in plasma factor VII was 95-100% of theoretical; the half-disappearance time was about 4 h. The treatment of four patients with acquired deficiency of factor VII is also described. No untoward side effects were observed.
D000328:Adult; D000368:Aged; D001780:Blood Coagulation Tests; D002847:Chromatography, Agarose; D005167:Factor VII / Q000302:isolation & purification*; D005168:Factor VII Deficiency / Q000628:therapy; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged
D005167:Factor VII