[Interaction of rabbit muscle creatine kinase with a reactive ATP derivative-ATP gamma-4(N-2-chloroethyl-N-methyl-amino)-benzylamidate].
The interaction of AGP gamma-4(N-2-chloroethyl-N-methyl-amino)-benzylamidate with rabbit muscle creatine kinase was studied. ATP gamma-4-(N-2-hydroxyethyl-N-methyl-amino)-benzylamidate acts as competitive inhibitor of creatine kinase. The Km value for ATP and the Ki value for the gamma-analog have been determined. A complete inactivation is observed when 1 mole of the reagent binds per 1 mole of the enzyme. The modification of the second subunit of creatine kinase is achieved at higher concentrations of the reactive ATP analog. The reactive ATP derivative is shown to be an affinity reagent for this enzyme. The possibility of interaction between the subunits of creatine kinase is discussed.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism*; D007700:Kinetics; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits; D013329:Structure-Activity Relationship
C025165:ATP-gamma-4-(N-2-chloroethyl-N-methylamino)benzylamidate; D000255:Adenosine Triphosphate; D003402:Creatine Kinase
Vzaimodeĭ kreatinkinazy iz myshts krolika s reaktsionnosposobnym proizvodnym ATP-4-(N-2-khlorztil-N-metilamino)-benzil-gamma-amidom ATP.