High-performance liquid chromatographic separation and isolation of quinidine and quinine metabolites in rat urine.
A procedure for the separation and isolation of the urinary metabolites of quinidine and quinine by reversed-phase high-performance liquid chromatography is described. Nine metabolites of quinidine and eight metabolites of quinine were detected in the urine of male Sprague-Dawley rats after a single dose of quinidine or quinine (50 mg kg-1). Following extraction from urine, the metabolites were separated on either an analytical or a semi-preparative reversed-phase column by gradient elution. After isolation and derivatization, the metabolites were analyzed by gas chromatography and gas chromatography--mass spectrometry.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D011802:Quinidine / Q000302:isolation & purification / Q000652:urine*; D011803:Quinine / Q000302:isolation & purification / Q000652:urine*; D051381:Rats
D011803:Quinine; D011802:Quinidine