Separation of human monocytes on density gradients of Percoll.
Monocytes from human blood have been isolated by centrifugation in Percoll. A one-step procedure has been designed to isolate the cells from 7 ml of blood. when 5% of the white blood cells are assumed to be monocytes, an estimated average yield of 100% and a purity of 20% is achieved. The contaminating cells are almost exclusively lymphocytes. By a two-step procedure the monocytes can be obtained 90% pure with an approximate yield of 35%. The cells can be used for tissue culture without washing and they display the usual properties of mononuclear phagocytes in vitro.
D001777:Blood Coagulation; D002448:Cell Adhesion; D002462:Cell Membrane / Q000502:physiology; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D006400:Hematocrit; D006801:Humans; D009000:Monocytes; D010587:Phagocytosis; D011951:Receptors, Complement; D013997:Time Factors
D011951:Receptors, Complement