The purification of a unique calcium-binding protein from Morris hepatoma 5123 tc.
A heat-stable Ca2+-binding protein was purified to homogeneity from Morris hepatoma 5123 tc. It had an apparent molecular weight of 11 000, and isoelectric point of 3.9, and bound two atoms of calcium per molecule of protein. The spectral and amino acid analysis indicated the tumour protein to be similar to the parvalbumins. This protein has been shown to be absent in liver.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002135:Calcium-Binding Proteins / Q000032:analysis* / Q000302:isolation & purification; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D006358:Hot Temperature; D007526:Isoelectric Point; D008114:Liver Neoplasms, Experimental / Q000032:analysis*; D008297:Male; D008970:Molecular Weight; D051381:Rats; D013050:Spectrometry, Fluorescence
D000596:Amino Acids; D002135:Calcium-Binding Proteins