Analysis of nuclear accumulation of influenza nucleoprotein antigen in the presence of p-fluorophenylalanine.
When p-fluorophenylalanine (FPA) was added to influenza virus RI/5+-infected cells 4 hr after infection, virus-specific proteins were synthesized but infectious progeny virus was not produced. In these cells, synthesis of viral RNA was strongly inhibited and nucleoprotein (NP) antigen was found predominantly in the nucleus in contrast to untreated cells in which NP antigen was distributed throughout the whole cell. The intracellular location and migration of NP were examined by isotope labeling followed by fractionation of infected cells. In untreated cells, a large portion of the NP was present in the cytoplasm and most of it was detected in the form of ribonucleoprotein (RNP). In contrast, in FPA-treated cells little viral RNP was detectable and NP was present predominantly in the nucleus in a nonassembled, soluble form. When FPA was removed from the culture, synthesis of viral RNA was soon restored and a large amount of viral RNP appeared in the cytoplasm; this was followed by the production of infectious virus. The results of the experiments suggest that the NP synthesized in the presence of FPA is not assembled into viral RNP because of the lack of available RNA, and such NP migrates readily into the nucleus and accumulates there.
D000956:Antigens, Viral; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects* / Q000378:metabolism; D009698:Nucleoproteins / Q000276:immunology / Q000378:metabolism*; D010649:Phenylalanine / Q000031:analogs & derivatives*; D012367:RNA, Viral / Q000096:biosynthesis; D012261:Ribonucleoproteins / Q000096:biosynthesis; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism*; D010135:p-Fluorophenylalanine / Q000494:pharmacology*
D000956:Antigens, Viral; D009698:Nucleoproteins; D012367:RNA, Viral; D012261:Ribonucleoproteins; D014764:Viral Proteins; D010649:Phenylalanine; D010135:p-Fluorophenylalanine