Stable RNA-DNA-RNA polymerase complexes can accompany formation of a single phosphodiester bond.
Incubation of RNA polymerase with poly[d(A-T)n] template results in a binary enzyme-DNA complex. Further addition of the dinucleotide UpA and [alpha-32P]UTP results in catalytic formation of the labeled trinucleotide UpApU until substrate exhaustion. In contrast, incubation of binary enzyme-DNA complexes with ApU and [alpha-32P]ATP results in labeled ApUpA formation to an extent that is stoichiometric with the amount of enzyme present despite an excess of substrates. The occurrence of ApUpA in a stable DNA-enzyme-RNA ternary complex is shown by gel exclusion chromatography, Millipore filtration, and the ability of ternary complexes to support subsequent RNA chain elongation. Radioactivity is not bound to Millipore filters when purified, labeled ApUpA is added to enzyme-DNA binary complexes. Hence, phosphodiester bond formation is required for stable ternary complex formation. The absence of the delta subunit of RNA polymerase or the addition of rifampicin to the reaction before ribonucleotide substrates results in catalytic ApUpA formation instead of stable ternary complexes.
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D007700:Kinetics; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D010755:Organophosphates; D011067:Poly dA-dT; D011089:Polydeoxyribonucleotides; D011485:Protein Binding
D009841:Oligonucleotides; D009843:Oligoribonucleotides; D010755:Organophosphates; D011089:Polydeoxyribonucleotides; D011067:Poly dA-dT; D012321:DNA-Directed RNA Polymerases