An electrochemical analysis of changes in properties of DNA caused by ionizing and ultraviolet radiations.
Electrooxidation and electroreduction of gamma- and u.v.-irradiated DNA were studied by means of differential pulse voltammetry at the graphite electrode and differential pulse polarography at the dropping mercury electrode. Two separated voltammetric oxidation peaks G and A were used for monitoring conformational changes in guanine . cytosoine (GC) and adenine . thyminde (AT) pairs respectively in irradiated double-stranded (ds) DNA. Pulse-polarographic reduction peak III was used for detection of denatured DNA in the irradiated samples of ds DNA. It was found that the heights of peaks G and A of ds DNA did not change with the radiation dose after relatively low doses of gamma- and u.v.-radiations (up to ca. 40 krads and 1 X 10(4) Jm-2, respectively, when no single-stranded (ss) DNA was detected in the irradiated DNA samples. After higher doses of radiation the occurrence of ss DNA or ss segments in the irradiated samples of ds DNA was accompanied by an increase of peaks G and A; however, peak A grew more rapidly with the increasing dose than peak G. It was concluded that the results obtained support the assumption, according to which regions of ds DNA rich in AT pairs are more susceptible to denaturation caused by gamma- and u.v.-radiations.
D003037:Cobalt Radioisotopes; D004247:DNA / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004563:Electrochemistry; D005720:Gamma Rays; D009690:Nucleic Acid Conformation / Q000528:radiation effects; D009691:Nucleic Acid Denaturation / Q000528:radiation effects; D014466:Ultraviolet Rays
D003037:Cobalt Radioisotopes; D004247:DNA