title: Plant tumor reversal associated with the loss of foreign DNA.
abstract: Transformation of plant tissues into crown gall tumors has been associated with the transfer of a portion of a tumor-inducing plasmid (Ti-plasmid) into plant DNA. Various laboratories have regenerated normal-appearing plants from a number of crown gall tumors. This study investigates the fate of the foreign DNA in a series of tissues derived from various parts of a plant regenerated from the tumor BT-37 by Braum and his coworkers. It was found that all the foreign DNA sequences were lost from tissues that had lost all their tumorous traits; whereas the plasmid DNA sequences were still present in tissues that appeared normal but still exhibited tumorous traits when returned to tissue culture media. From these studies it would appear that the presence of the Ti-plasmid sequences in the plant DNA is required for the maintenance of the transformed state.
mesh_terms: D004247:DNA / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics*; D010941:Plant Tumors / Q000209:etiology*; D010957:Plasmids
chemical_list: D004273:DNA, Neoplasm; D004247:DNA