Chemical synthesis of nucleoside-gamma-[32P]triphosphates of high specific activity.
A simple chemical procedure for the preparation of four common ribonucleoside 5-gamma-[32P]triphosphates of high specific activity (up to 10 Ci/mmole) based on the condensation of orthophosphoric acid with the corresponding nucleoside 5-diphosphate in the presence of ethyl chloroformate as well as the methods of purification and identification of the products are described.
D000255:Adenosine Triphosphate / Q000032:analysis / Q000138:chemical synthesis*; D003570:Cytidine Triphosphate / Q000032:analysis / Q000138:chemical synthesis*; D003597:Cytosine Nucleotides / Q000138:chemical synthesis*; D006160:Guanosine Triphosphate / Q000032:analysis / Q000138:chemical synthesis*; D007553:Isotope Labeling; D008722:Methods; D010761:Phosphorus Radioisotopes; D014500:Uracil Nucleotides / Q000138:chemical synthesis*; D014544:Uridine Triphosphate / Q000032:analysis / Q000138:chemical synthesis*
D003597:Cytosine Nucleotides; D010761:Phosphorus Radioisotopes; D014500:Uracil Nucleotides; D003570:Cytidine Triphosphate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D014544:Uridine Triphosphate