A simplified radioenzymatic assay for dihydrofolate reductase using [3H]dihydrofolate.
D004227:Dithionite / Q000494:pharmacology; D005492:Folic Acid / Q000031:analogs & derivatives*; D005590:Fractional Precipitation; D006801:Humans; D007700:Kinetics; D008722:Methods; D008727:Methotrexate / Q000494:pharmacology; D009249:NADP; D010084:Oxidation-Reduction; D013431:Sulfates; D013762:Tetrahydrofolate Dehydrogenase / Q000032:analysis*; D014316:Tritium; D015032:Zinc; D019287:Zinc Sulfate
D013431:Sulfates; D014316:Tritium; D004227:Dithionite; C010920:dihydrofolate; D009249:NADP; D019287:Zinc Sulfate; D005492:Folic Acid; D013762:Tetrahydrofolate Dehydrogenase; D015032:Zinc; D008727:Methotrexate