title: Assessment of fatty acids in dog left ventricular myocardium.
abstract: The concentration and composition of fatty acids in four lipid classes in biopsies of dog left ventricular myocardium were determined, using gas-liquid chromatography. When precautions were taken to minimize lipolysis during storage of the tissue and the homogenization process, the following results were obtained: 29 +/- 10 nmol non-esterified fatty acids, 2.98 +/- 2.41 mumol triacylglycerol, 149 +/- 51 nmol cholesteryl esters and 23.76 +/- 3.38 mumol phospholipid (expressed as fatty acid moiety per gram of wet tissue). The concentration of non-esterified fatty acids was 15 to 300 times lower than reported in literature. The main constituents of the non-esterified fatty acids were palmitic, stearic and oleic acid. Triacylglycerol consisted of approximately 40% esterified oleic acid. Linoleic acid accounted for 40% of the fatty acids in the cholesteryl-esters class. More than half of the fatty acid moiety of total phospholipids was linoleic acid and arachidonic acid.
mesh_terms: D000818:Animals; D002788:Cholesterol Esters / Q000032:analysis; D002849:Chromatography, Gas; D004285:Dogs; D005227:Fatty Acids / Q000032:analysis*; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005260:Female; D008297:Male; D009206:Myocardium / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D014021:Tissue Preservation; D014280:Triglycerides / Q000032:analysis
chemical_list: D002788:Cholesterol Esters; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D010743:Phospholipids; D014280:Triglycerides