Transsulfuration by human long term lymphoid lines. Normal and cystathionase-deficient cells.
Long term human lymphoid cells from normal subjects were able to convert [35S]homocysteine to [35S]-cystine. The labeled cystine was found incorporated into proteins. Cells cultured from a patient with vitamin B6-unresponsive cystathioninuria, which contain no measurable cystathionase activity, were unable to perform this conversion. This is the first direct demonstration of transsulfuration by diploid cells in culture.
D002460:Cell Line; D003542:Cystathionine gamma-Lyase / Q000172:deficiency / Q000378:metabolism*; D003553:Cystine / Q000096:biosynthesis*; D006710:Homocysteine / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008190:Lyases / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D013462:Sulfur Radioisotopes
D013462:Sulfur Radioisotopes; D006710:Homocysteine; D003553:Cystine; D008190:Lyases; D003542:Cystathionine gamma-Lyase