[Protein spectrum of rumen mucosa nuclear and mitochondrial fractions].
Using disc- and immunoelectrophoresis the protein spectrum of nuclear and mitochondrial fractions of the rumen mucosa and liver tissue was studied in growing bull calves feeding them various forms of the carbamide concentrate. The rumen mucosa nuclear fraction contains a less amount of water-soluble proteins but a greater amount of membrane proteins than the liver. In the rumen mucosa mitochondrial fraction the content of soluble and membrane proteins is considerably lower than in the liver. Soluble nuclear proteins of the rumen mucosa are more heterogeneous than those of the liver. No postablumins were revealed by disc-electrophoresis in the liver nuclei. Specific immunoglobulins were identified in the mucosa nuclear fraction by immunoelectrophoresis. The rumen mucosa mitochondrial fraction is characterized by the absence of postalbumins and transferrins in disc-electrophoregram. This post-transferrins of this tissue are shown to belong to specific immunoglobulins. Heterogeneity of albumin in the studied rumen mucosa subfractions is established. The extruded carbamide concentrate increases the content of membrane proteins in the nuclear and mitochondria fractions of bull calf rumen mucosa and changes the spectrum of soluble proteins of these subfractions.
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000032:analysis*; D004589:Electrophoresis, Disc; D007122:Immunoelectrophoresis; D007136:Immunoglobulins / Q000032:analysis; D008099:Liver / Q000032:analysis; D008297:Male; D008565:Membrane Proteins / Q000032:analysis; D008928:Mitochondria / Q000032:analysis*; D009092:Mucous Membrane / Q000032:analysis*; D009698:Nucleoproteins / Q000032:analysis; D009928:Organ Specificity; D011506:Proteins / Q000032:analysis*; D012417:Rumen / Q000032:analysis*
D007136:Immunoglobulins; D008565:Membrane Proteins; D009698:Nucleoproteins; D011506:Proteins
Issledovanie belkovogo spektra iadernoĭ i mitokhondrial'noĭ fraktsiĭ slizistoĭ obolochki rubtsa.