Hepatocyte-tumor cell interaction in vitro. I. Conditions for rosette formation and inhibition by anti-H-2 antibody.
Murine hepatocytes, isolated by an in situ collagenase-perfusion technique and cultured in Petri dishes, were shown to form rosettes with liver-metastasizing syngeneic tumor cells. Pretreatment of the tumor cells with neuraminidase generally increased the binding, whereas pretreatment of the liver cells with neuraminidase abolished the binding completely. The tumor-cell binding may be mediated by the previously described lectin-like receptor of hepatocytes that also was sensitive to neuraminidase treatment and that bound desialylated cells better than normal cells. Anti-H-2 sera could efficiently inhibit the rosette formation of metastatic tumor cells with the hepatocytes, which points to a possible role of H-2 molecules in this interaction of neoplastic and normal cells.
D000818:Animals; D000937:Antigen-Antibody Reactions; D002449:Cell Aggregation; D002478:Cells, Cultured; D006183:H-2 Antigens; D007518:Isoantibodies; D007519:Isoantigens; D008099:Liver / Q000473:pathology*; D008223:Lymphoma / Q000473:pathology; D051379:Mice; D009362:Neoplasm Metastasis; D009374:Neoplasms, Experimental / Q000473:pathology*; D012397:Rosette Formation
D006183:H-2 Antigens; D007518:Isoantibodies; D007519:Isoantigens