Genetic variation during lytic reovirus infection: high-passage stocks of wild-type reovirus contain temperature-sensitive mutants.
Wild-type clones of reovirus serotypes 1 (Lang), 2 (Jones), and 3 (Dearing) were serially passaged in L cells at a high multiplicity of infection, and the virus population was examined at passage levels 2, 5, and 11 for the presence of temperature-sensitive (ts) mutants. By passage 11 all three serotypes contained ts mutants that were not present in the original wild-type stock. ts mutants representing three mutant groups were identified. The majority of these mutants were in group G. Our results show that high-passage stocks of reovirus consist of a genetically heterogeneous population.
D005814:Genes, Viral; D012089:Mammalian orthoreovirus 3 / Q000235:genetics / Q000254:growth & development; D009154:Mutation; D012087:Reoviridae / Q000235:genetics* / Q000254:growth & development; D013696:Temperature; D013997:Time Factors; D014776:Virus Cultivation