Effect of bile lipids on the adsorption and activity of pancreatic lipase on triacylglycerol emulsions.
Emulsions of natural triacylglycerols obtained with different shear forces were used to study lipase adsorption and lipolysis. The influence of the bile lipoprotein complex on these two processes was determined. Optimal lipase activity was observed to occur with a given phospholipid : triacylglycerol ratio. This ratio depended on the degree of triacylglycerol emulsification and was accompanied by maximal adsorption of the bile lipoprotein complex. These results support our previous model for pancreatic lipolysis under physiological conditions, according to which colipase controls lipase binding to the bile lipoprotein complex and the resulting association directs enzyme adsorption to the acylglycerol particle (Lairon, D., Nalbone, G., Lafont, H., Léonardi, J., Domingo, N., Hauton, J.C. and Verger, R. (1978) Biochemistry 17, 5263--5269).
D000327:Adsorption; D000818:Animals; D001646:Bile / Q000378:metabolism*; D004655:Emulsions; D008049:Lipase / Q000378:metabolism*; D008055:Lipids / Q000494:pharmacology*; D008066:Lipolysis; D008074:Lipoproteins / Q000378:metabolism; D008954:Models, Biological; D010179:Pancreas / Q000201:enzymology*; D051381:Rats; D013552:Swine; D014280:Triglycerides / Q000378:metabolism
D004655:Emulsions; D008055:Lipids; D008074:Lipoproteins; D014280:Triglycerides; D008049:Lipase