title: Reticuloendothelial clearance of blood-borne particulates: relevance to experimental lung microembolization and vascular injury.
abstract: The rapid increase in sheep lung vascular permeability observed during Pseudomonas aeruginosa bacteremia may be due to embolization of the pulmonary microvasculature by bloodborne particulates. Since alterations in lung microvascular permeability during mild septicemia in sheep may reflect inefficient RES phagocytic clearance of bacteria as well as products of bacterial induced intravascular coagulation, the opsonic and phagocytic aspects of RES function in sheep (30-50 kg) were compared to other species. RES function was evaluated by both the clearance and relative organ uptake of gelatinized I(131) RE test lipid emulsion and gelatinized colloidal carbon. Immunoreactive opsonic a(2)SB glycoprotein levels were determined by electroimmunoassay. The phagocytic index for RES clearance of the gelatinized (500 mg/kg) test lipid in sheep was 0.019 +/- 0.002 corresponding to a half-time of 16.65 +/- 1.74 minutes. With colloidal carbon (64 mg/kg), the phagocytic index in sheep was 0.080 +/- 0.026, corresponding to a half-time of 6.16 +/- 1.99 minutes. The per cent of injected lipid emulsion (%ID) in major RE organs, on a total organ basis (TO), was: liver = 15.69 +/- 1.65%; spleen = 2.09 +/- 0.78%. Localization in the lung = 31.39 +/- 6.2%. The per cent of carbon localized in major RE organs (%ID/TO) was: liver = 21.37 +/- 1.9%; spleen = 1.95 +/- 0.55%. Localization in the lung = 32.70 +/- 4.55%. In contrast, clearance and organ distribution of the blood-borne test microparticles in rats and dogs at the same relative challenging dose revealed a much more intense and rapid liver and spleen RES uptake with minimal lung localization (1-2%). Immunoreactive opsonic protein concentrations varied greatly with species and directly correlated with efficiency of RES function. Levels observed were: dog = 1285 +/- 135 microg/ml; mouse = 1077 +/- 67 microg/ml; rat = 400 +/- 31 microg/ml; human = 297 +/- 10 microg/ml; and sheep = 184 +/- 13 microg/ml. After intravenous particulate challenge, circulating immunoreactive opsonic protein in the sheep was depleted (p < 0.05) rapidly with partial recovery at 24 hours and mild rebound hyperopsonemia at 48 hours. This pattern is in contrast to the rapid restoration seen in dog and rat within three to six hours postchallenge. Thus, in sheep, the extensive pulmonary localization of blood-borne microparticles appears related to inefficient RES clearance function mediated by a relative deficiency of circulating opsonic protein (plasma fibronectin).
mesh_terms: D000818:Animals; D001424:Bacterial Infections / Q000097:blood / Q000503:physiopathology; D002199:Capillary Permeability; D002244:Carbon / Q000097:blood*; D003102:Colloids; D004211:Disseminated Intravascular Coagulation / Q000209:etiology / Q000503:physiopathology*; D004285:Dogs; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D006801:Humans; D008055:Lipids / Q000097:blood*; D008099:Liver / Q000502:physiology; D008168:Lung / Q000098:blood supply / Q000502:physiology; D008297:Male; D051379:Mice; D012157:Mononuclear Phagocyte System / Q000502:physiology*; D009895:Opsonin Proteins / Q000032:analysis; D010587:Phagocytosis; D011655:Pulmonary Embolism / Q000503:physiopathology*; D051381:Rats; D012756:Sheep; D013154:Spleen / Q000502:physiology
chemical_list: D003102:Colloids; D005338:Fibrin Fibrinogen Degradation Products; D008055:Lipids; D009895:Opsonin Proteins; D002244:Carbon