On the influence of reaction conditions in activity determination of alkaline phosphatase on the molar absorptivity of 4-nitrophenol.
In activity determination of alkaline phosphatase (AP), measuring temperature, type and concentration of buffer, and protein concentration in the test influence the molar absorptivity of 4-nitrophenol. Thus systematic errors of up to 3% may occur in activity determinations of AP if these influences are not taken into account.
D000042:Absorption; D000469:Alkaline Phosphatase / Q000097:blood*; D002021:Buffers; D003951:Diagnostic Errors; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009596:Nitrophenols; D010783:Photometry; D012709:Serum Albumin; D012972:Sodium Hydroxide; D013696:Temperature
D002021:Buffers; D009596:Nitrophenols; D012709:Serum Albumin; D012972:Sodium Hydroxide; D000469:Alkaline Phosphatase