title: High-frequency heterokaryon formation by Mucor racemosus.
abstract: Heterokaryons from Mucor racemosus were produced from two auxotrophic strains of the fungus. Germlings were converted to spheroplasts by using commercial chitinase and purified Myxobacter AL-1 chitosanase. Spheroplasts from the auxotrophic strains were mixed and fused in solutions of polyethylene glycol and CaCl2. Under optimal conditions, prototrophic heterokaryons were formed at a frequency of about 5%.
mesh_terms: D002122:Calcium Chloride / Q000494:pharmacology; D002467:Cell Nucleus; D005816:Genetic Complementation Test; D006579:Heterozygote; D006824:Hybridization, Genetic; D006863:Hydrogen-Ion Concentration; D009089:Mucor / Q000235:genetics*; D011092:Polyethylene Glycols / Q000494:pharmacology; D013104:Spheroplasts / Q000235:genetics*
chemical_list: D011092:Polyethylene Glycols; D002122:Calcium Chloride