Onchocerca volvulus: effect of suramin on lactate dehydrogenase and malate dehydrogenase.
Lactate dehydrogenase and malate dehydrogenases were partially purified and characterized from adult O. volvulus. The molecular weight of lactate dehydrogenase was determined to be 130 000, those of malate dehydrogenase I and II to be 120 000 and 65 000, respectively. The activities of both malate dehydrogenases and of the lactate dehydrogenase were strongly inhibited by suramin. The inhibition constants were determined to be in the range of 2 microM to 5 microM. The type of inhibition was found to be competitive with respect to the coenzyme NADH and to be non-competitive to the substrates. It is suggested that the mode of action of suramin in the therapy of Onchocerciasis might depend on the blokkade of reoxidation of NADH produced within the glycolytic pathway.
D000818:Animals; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D009854:Onchocerca / Q000187:drug effects* / Q000201:enzymology; D013498:Suramin / Q000494:pharmacology*
D009243:NAD; D013498:Suramin; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase