Iron requirement for isolated rat liver mitochondrial protein synthesis.
Isolated rat liver mitochondrial protein synthesis was severly inhibited by alpha, alpha-dipyridyl (a ferrous iron-chelating agent), chloramphenicol and hemin (10(-7) M or greater). In contrast, gamma, gamma-dipyridyl (a non-iron-chelating analogue of alpha, alpha-dipyridyl), cycloheximide and lower concentrations of hemin were non-inhibitory. The inhibitory action of alpha, alpha-dipyridyl was reversed by addition of Fe(NH4)2(SO4)2 while ZnCl2, CuCl2 and CoCl2 were ineffective. Hemin, however, did not protect against the alpha, alpha-dipyridyl inhibition of mitochondrial protein synthesis. These results indicate that ferrous iron is required for mitochondrial protein synthesis and suggests that it is through a mechanism independent of hemin concentration.
D015082:2,2'-Dipyridyl / Q000494:pharmacology; D000818:Animals; D002701:Chloramphenicol / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology; D006427:Hemin / Q000494:pharmacology; D007425:Intracellular Membranes / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis; D051381:Rats
D005296:Ferrous Compounds; D015082:2,2'-Dipyridyl; D002701:Chloramphenicol; D006427:Hemin; D003513:Cycloheximide; D007501:Iron