The effect of lithium on the incorporation of 32P-orthophosphate into synaptosomal phospholipids from rat brain.
Rats were treated with lithium added to the diet for five weeks (40 mmol LiCl/kg diet). The mean plasma lithium concentration was 0.48 mmol/l plasma, and the blood was drawn at 8 a.m. The brains were removed and synaptosomes were prepared according to the method of Gray & Whittaker (1962) and Bradford (1972). The synaptosomes were incubated for 120 min. with 32P-orthophosphate, either in a lithium-containing medium or in a lithium-free medium. The 32P-incorporation was lower in the synaptosomes from lithium-treated rats than the 32P-incorporation in synaptosomes from control rats regardless of the medium chosen. The results indicate that lithium treatment in vivo decreases the 32P-incorporation into synaptosomal phospholipids and that the effect remains after the removal of the lithium ion.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D008094:Lithium / Q000097:blood / Q000494:pharmacology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D010710:Phosphates; D010743:Phospholipids; D008094:Lithium