[Influence of monodehydroascorbate on the acyl-CoA-desaturase system of rat liver microsomes (author's transl)].
The microsomal NADH- as well as the ascorbate-dependent fatty acid desaturation are diminished by monodehydroascorbate. This effect can be neutralized by borate. Borate forms an adduct with didehydroascorbate and thus prevents the formation of monodehydroascorbate. The ascorbate-dependent oleate formation is increased significantly by the same reaction (p less than 0.01). The NADH-dependent reaction is diminished by parallel reactions of the desaturase system and L-ascorbate: ferricytochrome b5 oxidoreductase at ferrocytochrome b5. In the case of the ascorbate-dependent reaction the diminished oleate formation is caused by the more positive oxidation state of cytochrome b5 in presence of monodehydroascorbate.
D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D003683:Dehydroascorbic Acid / Q000494:pharmacology*; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats
D044943:Fatty Acid Desaturases; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid
Einfluss von Monodehydroascorbat auf das mikrosomale Acyl-CoA-Desaturase-System der Rattenleber.