Circular dichroism studies of M and N blood group-specific glycoproteins and glycopeptides.
CD spectra of M and N blood group glycoproteins and their NH2-terminal tryptic glycopeptides were compared. The CD spectra of glycoproteins obtained suggest the presence of a low content of alpha-helix. The M glycoprotein contained a higher percentage of a periodical structure. CD spectra of M and N tryptic glycopeptides, both sialoglycopeptides and desialylated preparates, were similar and showed positive ellipticities in the range of 210--240 m. However, the spectra of desialylated glycopeptides were red-shifted in respect to spectra of sialoglycopeptides. The CD spectra of glycopeptides suggest the presence of beta-turns.
D002942:Circular Dichroism; D006020:Glycopeptides / Q000097:blood*; D006021:Glycophorins; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D008951:MNSs Blood-Group System; D011487:Protein Conformation; D012795:Sialoglycoproteins / Q000097:blood; D014357:Trypsin
D006020:Glycopeptides; D006021:Glycophorins; D006023:Glycoproteins; D008951:MNSs Blood-Group System; D012795:Sialoglycoproteins; D014357:Trypsin