title: Characterization of guinea pig lymphocyte-derived chemotactic factor for fibroblasts.
abstract: An assay that measures guinea pig fibroblast chemotaxis in vitro is described. Application of this assay has revealed that stimulation of guinea pig blood or lymph node lymphocytes in vitro by T cell antigen (horseradish peroxidase) or mitogen (phytohemagglutinin) is associated with production of a chemoattractant for guinea pig fibroblasts. This lymphocyte-derived chemotactic factor for fibroblasts (LDCF-F) has a m.w. or 80,000 and is distinct from guinea pig lymphocyte-derived chemotactic factor for monocytes (LDCF-M). LDCF-F is heat labile and trypsin sensitive. The production of LDCF-F in vitro correlates with lymphocyte transformation to antigen or mitogen and with the presence of in vivo-delayed hypersensitivity to antigen. LDCF-F might function in vivo to attract connective tissue fibroblasts to sites of cell-mediated immune reactions.
mesh_terms: D000818:Animals; D002465:Cell Movement; D002630:Chemotactic Factors / Q000096:biosynthesis*; D002850:Chromatography, Gel; D003182:Complement C5 / Q000276:immunology; D005347:Fibroblasts / Q000276:immunology; D006168:Guinea Pigs; D006358:Hot Temperature; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis*; D014357:Trypsin / Q000494:pharmacology
chemical_list: D002630:Chemotactic Factors; D003182:Complement C5; D007106:Immune Sera; D008222:Lymphokines; D014357:Trypsin