A clinical evaluation of a card agglutination test for rheumatoid factor.
The Macro-Vue Card Test for Rheumatoid Factor (Hynson, Westcott, and Dunning) is a new commercially available card agglutination test designed for qualitative and quantitative procedures. Coded sera from 195 patients, six known positive sera, and two positive serum pools were tested using four commercial kits. The commercial kits evaluated included latex (Hyland RA Test, Behring-Rapi/tex RF Test), sheep erythrocytes (Wampole, Rheumaton), and charcoal (HWD-Macro-Vue RF Card Test) as carrier particles. Using the prescribed quantitative procedures, the percentages of positive and negative results for rheumatoid factor detected by each product were determined after the (serum) code was broken. The Macro-Vue test compares well with the Behring and Hyland latex dilution systems, and appears to detect more positive sera than the Wampole test. Titers obtained using the Macro-Vue test were more reproducible day to day with known positive sera than were titers obtained using any of the other kits.
D001172:Arthritis, Rheumatoid / Q000097:blood*; D005188:False Negative Reactions; D005189:False Positive Reactions; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D012217:Rheumatoid Factor / Q000032:analysis*
D012217:Rheumatoid Factor