Inhibition or killing of an intracellular pathogen by activated macrophages is abrogated by TLCK or aminophylline.
Agents that are known to inhibit certain metabolic pathways or cell functions were evaluated to determine their effects on the capacity of activated macrophages to inhibit or kill obligate intracellular pathogens. Tosyllysine chloromethyl ketone (TLCK) and aminophylline abrogate inhibition or killing of Toxoplasma gondii by activated macrophages.
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology*; D000628:Aminophylline / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D008264:Macrophages / Q000187:drug effects / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology*; D014122:Toxoplasma / Q000254:growth & development; D014124:Toxoplasmosis, Animal / Q000276:immunology* / Q000382:microbiology
D000590:Amino Acid Chloromethyl Ketones; D014107:Tosyllysine Chloromethyl Ketone; D000628:Aminophylline