[Effect of lactate and glycolytic intermediates on muscle creatine kinase].
Inhibition of rabbit muscle creatine kinase by lactate, glucose-6-phosphate and PEP occurs immediately and depends on the concentration of the effectors and enzyme properties. Desensitization of creatine kinase to these effectors is described. Partial desensitization to G-6-P and lactate occurs after the enzyme preincubation with dithiothreitol. A complete loss of sensitivity to lactate and a significant loss of sensitivity to G-6-P and PEP occurs after the enzyme storage at -15 degrees during several weeks. The data obtained support our previously made assumption on the allosteric regulation of the MM isoenzyme of creatine kinase.
D000494:Allosteric Regulation; D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism*; D004229:Dithiothreitol / Q000494:pharmacology; D005615:Freezing; D005958:Glucosephosphates / Q000494:pharmacology*; D006019:Glycolysis; D007527:Isoenzymes; D007700:Kinetics; D007773:Lactates / Q000494:pharmacology*; D009132:Muscles / Q000201:enzymology*; D010728:Phosphoenolpyruvate / Q000494:pharmacology*; D011817:Rabbits
D005958:Glucosephosphates; D007527:Isoenzymes; D007773:Lactates; D010728:Phosphoenolpyruvate; D003402:Creatine Kinase; D004229:Dithiothreitol
Issledovanie deĭstviia laktata i glikoliticheskikh intermediatov na myshechnuiu kreatinkinazu.