Quantitative determination of retinals with complete retention of their geometric configuration.
A method is described for the quantitative extraction of retinal in its original isomeric configuration from retinal-containing pigments. Using excess of hydroxylamine under denaturing conditions, the chromophore of retinal bearing natural products is converted into the corresponding retinaloxime with complete retention of geometric configuration. The retinaloximes can be quantitatively extracted with dichloromethane and analyzed by high-performance liquid chromatography.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006898:Hydroxylamines; D008027:Light; D010786:Photoreceptor Cells / Q000032:analysis; D012172:Retinaldehyde / Q000032:analysis*; D012243:Rhodopsin / Q000032:analysis; D013237:Stereoisomerism; D014801:Vitamin A / Q000031:analogs & derivatives*
D006898:Hydroxylamines; D014801:Vitamin A; D012243:Rhodopsin; D012172:Retinaldehyde