Early induction of mouse urinary bladder ornithine decarboxylase activity by rodent vesical carcinogens.
The responses of mouse urinary bladder ornithine decarboxylase (EC 4.1.1.17) and S-adenosyl-L-methionine decarboxylase (EC 4.1.1.50) activities were studied following topical intravesical administration of N-[4-(5-nitro-2-furyl)-2-thiazolyl]-formamide (FANFT) or 2-amino-4-(5-nitro-2-furyl)thiazole (ANFT), potent rodent bladder carcinogens. A single bladder topical application of ANFT or FANFT resulted in a significant increase over controls of ornithine decarboxylase activity within 5 hr, with a return to control levels by 10 hr. S-Adenosyl-L-methionine decarboxylase activity demonstrated a lesser response to topical ANFT or FANFT, achieving a level 2 or 3 times that of controls at 5 to 8 hr, followed by a gradual decline to control levels. Stimulation of activities of both enzymes was dose dependent over a range of 4.6 to 460 nmol of ANFT. ANFT-induced ornithine decarboxylase activity was principally localized in the bladder epithelium and was inhibited in a linear dose-response relationship by the synthetic retinoid, 13-cis-retinoic acid. Mice given FANFT p.o. demonstrated a significant increase over controls in ornithine decarboxylase activity within 12 hr, followed by a gradual decline to control levels by 72 hr.
D012437:Adenosylmethionine Decarboxylase / Q000096:biosynthesis; D000818:Animals; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D002273:Carcinogens / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D004848:Epithelium / Q000201:enzymology; D005200:FANFT / Q000031:analogs & derivatives / Q000494:pharmacology*; D005260:Female; D051379:Mice; D009374:Neoplasms, Experimental / Q000139:chemically induced; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D013844:Thiazoles / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology; D001743:Urinary Bladder / Q000201:enzymology*; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced
D002273:Carcinogens; D013844:Thiazoles; C024885:ANFT; D014212:Tretinoin; D005200:FANFT; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase