Effect of discrete kainic acid-induced lesions of corpus caudatus and globus pallidus on glutamic acid decarboxylase of rat substantia nigra.
Locally applied kainic acid was used in order to destroy pallidal perikarya without damaging axons en passage, in an effort to clarify the role of the globus pallidus as a source of nigral GABAergic terminals. Rats were microinjected unilaterally with kainic acid in the globus pallidus, head, body and tail of the caudate and were sacrificed 7 days later. The forebrain of each rat was examined histologically in order to establish the extent of the lesion and nigral glutamate decarboxylase (GAD) was assayed as a marker of GABAergic terminals. Kainic acid produced in the globus pallidus loss of neuronal perikarya and reactive gliosis. Large multipolar neurons of the globus pallidus were characteristically absent on the lesioned-side. Lesions of the pallidum resulted in a non-significant (5.5%) reduction of nigral GAD. Kainate lesions restricted to the head of the caudate resulted in a significant (19%) drop of nigral GAD, while lesions of the caudate body provided the largest reductions of nigral GAD (53%). Lesions of the caudate tail were without effect. The results indicate that nigral GAD arises mostly from the body and, in part, also from the head of the caudate but not from the globus pallidus or from the tail of the caudate.
D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D002421:Caudate Nucleus / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D005917:Globus Pallidus / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology*; D005968:Glutamate Decarboxylase / Q000378:metabolism*; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D011759:Pyrrolidines / Q000494:pharmacology*; D051381:Rats; D013378:Substantia Nigra / Q000033:anatomy & histology / Q000187:drug effects / Q000201:enzymology*
D011759:Pyrrolidines; D002262:Carboxy-Lyases; D005968:Glutamate Decarboxylase; D007608:Kainic Acid