[Differences in the levels of serum immunoglobulin E determined by the competitive radio-immuno-sorbent and the paper-radioimmuno-sorbent methods: Removal of these differences by pretreatment of sera with polyethylene glycol (author's transl)].
The serum IgE of patients with chronic nonspecific lung diseases was determined with the competitive radio-immuno-sorbent and the paper-radio-immuno-sorbent methods (Phadebas). Some samples showed markedly higher values with the competitive radio-immuno-sorbent than with the paper-radio-immuno-sorbent method. These discrepancies were abolished by pretreatment of the sera: polyethylene glycol 6000 (final concentration 35 g/l) was added, followed by centrifugation at 0 degrees C for 30 min at 20 000 g, and IgE was determined in the supernatant. This treatment had no effect on the values of the paper-radio-immuno-sorbent method, but the values of the competitive radio-immuno-sorbent method were decreased so that the two values agreed within the experimental confidence limits.
D001667:Binding, Competitive; D002498:Centrifugation; D005189:False Positive Reactions; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D008171:Lung Diseases / Q000097:blood; D010209:Paper; D011092:Polyethylene Glycols; D011864:Radioimmunosorbent Test / Q000379:methods
D007073:Immunoglobulin E; D011092:Polyethylene Glycols
Unterschiede für Immunglobulin E im Serum, bestimmt mit kompetitivem bzw. Papier-Radio-immunosorbenttest: Beseitigung durch Vorbehandlung mit Polyethylenglycol.