title: The influence of bile salts and bile lipoprotein complex on pancreatic lipase hydrolysis of monomolecular films.
abstract: We report a new technique which allows us to follow the lipolysis of monomolecular films in the presence of bile salts by using a 'zero-order' trough (Verger, R. and de Haas, G.H. (1973) Chem. Phys. Lipids 10, 127). The effects of bile salts, the bile lipoprotein complex and colipase on pancreatic lipase hydrolysis of rac-1,2-didodecanoylglycerol films were studied at different surface pressures. Taking into account previous studies, lipase activity was interpreted as a function of its degree of binding to the bile lipoprotein complex.
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000494:pharmacology*; D003089:Colipases / Q000494:pharmacology; D004075:Diglycerides; D006868:Hydrolysis; D066298:In Vitro Techniques; D007700:Kinetics; D007848:Laurates; D008049:Lipase / Q000378:metabolism*; D008074:Lipoproteins / Q000494:pharmacology*; D008081:Liposomes; D010179:Pancreas / Q000201:enzymology*; D051381:Rats; D013552:Swine
chemical_list: D001647:Bile Acids and Salts; D003089:Colipases; D004075:Diglycerides; D007848:Laurates; D008074:Lipoproteins; D008081:Liposomes; C025405:1,2-didodecanoylglycerol; D008049:Lipase