Sleep-promoting material from human urine and its relation to factor S from brain.
A sleep-promoting factor was extracted from human urine. Intraventricular infusion of the purified material induced excess slow-wave sleep in rats and rabbits for 5--10 h after the infusion. Chemical properties of the urinary factor were similar to those of factor S derived from whole brains of sleep-deprived goats, sheep, and rabbits. The behavior of the urinary factor in two ion exchange chromatographic steps, high voltage electrophoresis, gel-filtration, and ultrafiltration was similar to that of factor S. Effects of the purified urinary factor on slow-wave sleep of rats and rabbits were similar in time-course and duration to those of factor S from brain. However, the factor obtained from human urine did not increase the amplitude of cortical slow waves to the same extent as did factor S from brains of sleep-deprived animals.
D000818:Animals; D001681:Biological Assay; D001921:Brain / Q000502:physiology; D001923:Brain Chemistry; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004569:Electroencephalography; D004590:Electrophoresis, Paper; D005450:Fluorescamine / Q000378:metabolism; D006801:Humans; D008297:Male; D010455:Peptides / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012890:Sleep / Q000502:physiology*; D014462:Ultrafiltration; D014556:Urine / Q000032:analysis*
D010455:Peptides; D005450:Fluorescamine