Fluorometric estimation of dead cells in cell suspensions.
An objective vitality test is proposed. It is based on the fluorescence increment of ethidium bromide in the presence of dead cells, which is proportional to cellular DNA under conditions previously defined.
D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000032:analysis; D006651:Histocytochemistry; D051379:Mice; D009381:Neoplasms, Radiation-Induced / Q000503:physiopathology; D012513:Sarcoma, Experimental / Q000503:physiopathology*; D013050:Spectrometry, Fluorescence / Q000379:methods; D014466:Ultraviolet Rays
D004273:DNA, Neoplasm