Cholic acid binding by glutathione S-transferases from rat liver cytosol.
Cholic acid-binding activity in cytosol from rat livers appears to be mainly associated with enzymes having glutathione S-transferase activity; at least four of the enzymes in this group can bind the bile acid. Examination of the subunit compositions of different glutathione S-transferases indicated that cholic acid binding and the ability to conjugate reduced glutathione with 1,2-dichloro-4-nitrobenzene may be ascribed to different subunits.
D000818:Animals; D002793:Cholic Acids / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D003600:Cytosol / Q000201:enzymology; D004137:Dinitrochlorobenzene / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005982:Glutathione Transferase / Q000302:isolation & purification / Q000378:metabolism*; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology*; D051381:Rats
D002793:Cholic Acids; D004137:Dinitrochlorobenzene; D007527:Isoenzymes; D005982:Glutathione Transferase