A centrifugal column assay for thymidylate synthetase using the active site titrant 5-fluoro-2'-deoxyuridylate.
D000818:Animals; D002498:Centrifugation / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D003856:Deoxyuracil Nucleotides; D004591:Electrophoresis, Polyacrylamide Gel; D005468:Fluorodeoxyuridylate; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008780:Methyltransferases / Q000032:analysis*; D051379:Mice; D011489:Protein Denaturation; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013940:Thymidylate Synthase / Q000032:analysis*
D003856:Deoxyuracil Nucleotides; D005468:Fluorodeoxyuridylate; D012967:Sodium Dodecyl Sulfate; D008780:Methyltransferases; D013940:Thymidylate Synthase