Eosinophil response in guinea pig skin to a low molecular weight eosinophil chemotactic factor extracted from livers of mice with schistosomiasis.
A low-molecular weight eosinophil chemotactic factor (ECF-G), isolated and partially purified from livers of mice with schistosomiasis, was injected intradermally into guinea pigs. Biopsies obtained were studied for inflammatory cell accumulation in the injected sites and compared with those in the control sites injected with phosphate buffered saline. Tissue eosinophilia was seen as early as 1 hr after injection of ECF-G, but not in the control site. The increase of eosinophilia appeared biphasic with peaks at 6 and 24 hr. Mast cells increased in both ECF-G and saline injected sites and the increase was still found at 120 hr after injection. Neutrophils also increased in both ECF-G and saline injected sites but disappeared within 48 hr. These findings indicate that ECF-G is a tissue and species nonspecific eosinophil chemotactic factor, and injection of ECF-G initiates interaction of eosinophils and mast cells in the skin.
D000818:Animals; D002452:Cell Count; D002630:Chemotactic Factors / Q000494:pharmacology*; D002631:Chemotactic Factors, Eosinophil / Q000302:isolation & purification / Q000494:pharmacology*; D004804:Eosinophils; D006168:Guinea Pigs; D007249:Inflammation / Q000473:pathology; D008099:Liver / Q000032:analysis / Q000276:immunology; D008297:Male; D008407:Mast Cells; D051379:Mice; D009504:Neutrophils; D012552:Schistosomiasis / Q000276:immunology; D012867:Skin / Q000187:drug effects* / Q000473:pathology
D002630:Chemotactic Factors; D002631:Chemotactic Factors, Eosinophil