Relative contribution of parenchymal and non-parenchymal cells to the hepatic distribution and metabolism of iron-poly(sorbitol-gluconic acid) complex in the rat.
The hepatic cellular distribution in rats of radioiron and radiocarbon after [14C-gluconic acid]-and 59Fe-labelled iron-poly(sorbitol-gluconic acid) complex, glusoferron (Ferastral), has been investigated. Following administration of the 59Fe-iron complex at a dose level corresponding to 10 mg of iron per kg bodyweight, the ratio of radioactivity per 10(8) cells in parenchymal (P) and non-parenchemal (NP) cell fractions, the P/NP ratio, was 3.0 after 24 hours and rose to 10.4 at day 14. After 100 mg/kg the ratio was 0.9, 10.5 and 5.7 at day 1, 4 and 14, respectively. Radiocarbon content in the different cell compartments fell steadily throughout. Radioiron from 59Fe-iron-poly(sorbitol-gluconic acid) complex was shown to be incorporated into whole liver 59Fe-ferritin. In vitro uptake studies with the different liver cells were also performed. Parenchymal cells were found to be more active than non-parenchymal cells with regard to cellular uptake of radiolabel from both iron complex and polymeric ligand without iron.
D000818:Animals; D002452:Cell Count; D002850:Chromatography, Gel; D004705:Endocytosis / Q000187:drug effects; D005293:Ferritins / Q000032:analysis; D007505:Iron-Dextran Complex / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats
D007505:Iron-Dextran Complex; D005293:Ferritins