Demonstration of enzymes in cells cultured on semipermeable membrane in a double chamber.
The description is presented of a simple device for short-term cell culturing on semipermeable membrane for the histochemical demonstration of total enzyme activities. The results are given of lysosomal enzyme detection in HeLa cells.
D000135:Acid Phosphatase / Q000378:metabolism; D002478:Cells, Cultured; D005696:Galactosidases / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D006367:HeLa Cells / Q000201:enzymology*; D006596:Hexosaminidases / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D006867:Hydrolases / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology; D008361:Mannosidases / Q000378:metabolism; D008722:Methods
D006867:Hydrolases; D000135:Acid Phosphatase; D005696:Galactosidases; D005959:Glucosidases; D006596:Hexosaminidases; D008361:Mannosidases