title: An agarose plate method for detecting alloantisera to coagulant factor IX and factor IX antigen.
abstract: An agarose plate method for detecting human alloantibodies (inhibitors) to coagulant factor IX has been developed. The assay is based on the ability of such antisera to inhibit the coagulation of a mixture of haemophilia B plasma, normal plasma and platelet subsitute in an agarose matrix. The agarose plate method was also adopted to measure levels of FIX antigen (IX:Ag) in plasma. Using this technique, 12 of 17 obligate carriers of haemophilia B demonstrated reduced levels of IX:Ag. Three of the five carriers with normal IX:Ag levels were members of kindred in which affected individuals had normal or near normal levels of IX:Ag.
mesh_terms: D000941:Antigens / Q000032:analysis / Q000276:immunology; D001780:Blood Coagulation Tests / Q000379:methods*; D005164:Factor IX / Q000276:immunology*; D005260:Female; D006580:Genetic Carrier Screening; D002836:Hemophilia B / Q000235:genetics / Q000276:immunology; D006801:Humans; D007518:Isoantibodies / Q000032:analysis* / Q000276:immunology; D008297:Male; D012685:Sepharose
chemical_list: D000941:Antigens; D007518:Isoantibodies; D005164:Factor IX; D012685:Sepharose