Simultaneous liquid-chromatographic determination of zinc protoporphyrin IX, protoporphyrin IX, and coproporphyrin in whole blood.
We describe a method for simultaneously measuring concentrations of coproporphyrin, zinc protoporphyrin IX, and protoporphyrin IX in whole blood by liquid chromatography, with use of reversed-phase ion-pair system, fluorometric detection, and internal standardization. Each analysis requires 10 microL of whole blood and 15 min total analysis time. Analytical recovery ranged from 84 to 92%, day-to-day precision (CV) from 5 to 12%. Uroporphyrin, though not studied in detail, can also be detected by this method.
D002853:Chromatography, Liquid / Q000379:methods*; D003306:Coproporphyrins / Q000097:blood*; D005470:Fluorometry; D006801:Humans; D011166:Porphyrins / Q000097:blood*; D011524:Protoporphyrins / Q000097:blood*; D015032:Zinc / Q000097:blood*
D003306:Coproporphyrins; D011166:Porphyrins; D011524:Protoporphyrins; D015032:Zinc