Effect of sex hormones on glucose-6-phosphate dehydrogenase in rat levator ani muscle.
We studied the influence of sex hormones using the hormone-sensitive levator ani muscle as a model tissue and glucose-6-phosphate dehydrogenase as an indicator of hormone action. Injection of testosterone or estradiol cause a 50% increase in the specific activity of glucose-6-phosphate dehydrogenase. The effect was dose-dependent, and was maximal at a dose of 2.5mg/100g body weight. Estradiol increased glucose-6-phosphate dehydrogenase as early as 8 h after injection, while testosterone required 12 h. Injection of estradiol on 2 successive days increased enzyme activity by 80%. The effect of estradiol was abolished by actinomycin D, suggesting enzyme induction. The results indicate a direct effect of estrogen on striated muscle.
D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000008:administration & dosage / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D013739:Testosterone / Q000008:administration & dosage / Q000494:pharmacology*; D013997:Time Factors
D003609:Dactinomycin; D013739:Testosterone; D004958:Estradiol; D005954:Glucosephosphate Dehydrogenase