title: Reversible transport defects of virus membrane glycoproteins in Sindbis virus mutant infected cells.
abstract: Surface immunofluorescence with anti-envelope serum was used to study the transport of virus membrane glycoproteins in cells infected with temperature-sensitive mutants of Sindbis virus. Mutants belonging to complementation group D (ts-10 and ts-23), which have a defect in the envelope protein E1, showed a temperature dependent defect in the transport of the virus glycoproteins to the cell surface. In contrast, cells infected with mutant ts-20, the only representative of complementation group E, with a defect in the pE2 protein, showed reduced surface fluorescence at the restrictive temperature.
mesh_terms: D000818:Animals; D001692:Biological Transport; D002478:Cells, Cultured / Q000378:metabolism; D002642:Chick Embryo; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D012845:Sindbis Virus / Q000235:genetics* / Q000378:metabolism; D013696:Temperature; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins; D014764:Viral Proteins