title: Control of membrane polar lipid composition in Acholeplasma laidlawii a by the extent of saturated fatty acid synthesis.
abstract: The low level of endogenous fatty acid synthesis in Acholeplasma laidlawii A strain EF22 was found to be caused by a deficiency of pantetheine in the lipid-depleted growth medium. By supplementing the oleic acid-containing medium with increasing concentrations of pantethein, saturated fatty acid synthesis was stimulated (having an apparent Km of 5 microM for pantetheine) and the incorporation of endogenously synthesized fatty acids in membrane lipids increased markedly. Furthermore, carotenoid biosynthesis was stimulated. Exogenous palmitic acid was found to inhibit partially the endogenous fatty acid synthesis. A gradual stimulation of fatty acid synthesis was accompanied by a linear increase in the molar proportion between the two dominating membrane glucolipids, monoglucosyldiacylglycerol and diglucosyldiacylglycerol. The total amount of charged membrane lipids decreased upon increasing the degree of fatty acid saturation. These regulations are discussed in terms of membrane stability, and influence of membrane molecular ordering and surface charge density on lipid polar head group synthesis.
mesh_terms: D000128:Acholeplasma laidlawii / Q000187:drug effects / Q000378:metabolism*; D002462:Cell Membrane / Q000032:analysis; D005227:Fatty Acids / Q000096:biosynthesis*; D006017:Glycolipids / Q000096:biosynthesis; D007700:Kinetics; D008563:Membrane Lipids / Q000032:analysis*; D010204:Pantetheine / Q000494:pharmacology
chemical_list: D005227:Fatty Acids; D006017:Glycolipids; D008563:Membrane Lipids; D010204:Pantetheine