Kinetic determination of serum glucose by use of the hexokinase/glucose-6-phosphate dehydrogenase method.
We have developed a kinetic fixed-time approach for the quantitative determination of serum glucose by use of the hexokinase/glucose-6-phosphate dehydrogenase method. To achieve a large measuring range, we have apparently increased the Michaelis constant of glucose-6-phosphate dehydrogenase through addition of the competitive inhibitor ATP. By this means, serum samples with glucose concentrations up to 55.5 mmol/l could be analyzed without pre-dilution. The method has been adapted to the ENI GEMSAEC analyzer and to the LKB 2086 Mark II analyzer. It yielded satisfactory results with regard to precision. A comparison of the kinetic procedure with the manual end-point method showed good agreement. No interferences from hemoglobin, bilirubin, or lipemia have been observed.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D001322:Autoanalysis / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D006801:Humans; D007700:Kinetics; D013223:Statistics as Topic
D001786:Blood Glucose; D000255:Adenosine Triphosphate; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase