Effect of apoproteins on hepatic uptake of triglyceride emulsions in the rat.
The addition of apoprotein E isolated from human very low density lipoproteins to both rat lymph chylomicrons and a triglyceride emulsion significantly increased the hepatic uptake of these particles in a nonrecycling isolated rat liver perfusion system. The cleared triglyceride was removed without apparent hydrolysis by the hepatocyte. When lymph chylomicrons were loaded with both Apo E and Apo C proteins by exposure to rat plasma, no increment in hepatic clearance was observed. Sequential evalutions of the influence of the C apoproteins on the hepatic clearance of both emulsions and chylomicrons revealed that the CIII (CIII-1) protein had a pronounced inhibitory effect on hepatic removal. The inhibition was observed for both Apo E-enriched chylomicrons and those containing little of this apoprotein.
D000818:Animals; D001053:Apolipoproteins / Q000378:metabolism / Q000494:pharmacology*; D002914:Chylomicrons / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008075:Lipoproteins, HDL / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008196:Lymph / Q000378:metabolism; D051381:Rats; D014280:Triglycerides / Q000378:metabolism*
D001053:Apolipoproteins; D002914:Chylomicrons; D008075:Lipoproteins, HDL; D014280:Triglycerides