[Properties of alpha-chymotrypsin, covalently incorporated into polyacrylamide microgranules].
Polyacrylamide beads (microgranules) containing covalently bound alpha-chymotrypsin were obtained. An average diameter of the microgranules is 100--150 micrometers. Prior to the bead polymerization alpha-chymotrypsin was modified by acryloyl chloride. The efficiency of the microgranulated alpha-chymotrypsin action depends on the kinetic properties of the substrates used. The pH optimum for the microgranulated enzyme activity towards N-acetyl-L-tyrosine ethyl ester is shifted by 1,5 units to the alkaline zone. The immobilized alpha-chymotrypsin is completely stable for 100 hrs at 50 degrees.
D000180:Acrylic Resins; D002918:Chymotrypsin / Q000378:metabolism*; D004355:Drug Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007700:Kinetics; D013379:Substrate Specificity; D013696:Temperature
D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D002918:Chymotrypsin
Svoĭstva al'fa-khimotripsina, kovalentno vkliuchennogo v sferncheskie mikrogranuly iz poliakrilamida.