Restricted subpopulations of DNA antibodies in kidneys of mice with systemic lupus. Comparison of antibodies in serum and renal eluates.
Sera from MRL/1, BXSB, and NZB/NZW mice, which develop IgG antibodies to DNA and glomerular deposits of DNA-antiDNA immune complexes, were studied by isoelectric focusing. A large array of IgG antibodies with isoelectric points ranging from pH 5.5--9.0 were found to bind double-stranded DNA. Antibodies with isoelectric points from 8.0--8.5 were significantly more frequent than antibodies focusing in all other pH ranges. In contrast, glomerular eluates from MRL/1 and NZB/NZW mice contained a restricted number of DNA-binding bands, all of which focused at pH 8.0--9.0. Anti-DNA with isoelectric points from pH 8.0--9.0 may be more pathogenic for the kidney than other subpopulations.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex; D004247:DNA / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification; D007525:Isoelectric Focusing; D007668:Kidney / Q000276:immunology / Q000503:physiopathology; D008180:Lupus Erythematosus, Systemic / Q000235:genetics* / Q000276:immunology; D008297:Male; D051379:Mice / Q000235:genetics* / Q000276:immunology; D011507:Proteinuria / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D004247:DNA