title: Kinetics of DNA replication in the Indian muntjac chromosomes as studied by quantitative autoradiography.
abstract: DNA replication patterns of individual chromosomes and their various euchromatic and heterochromatic regions were analyzed by means of quantitative autoradiography. The cultured cells of the skin fibroblast of a male Indian muntjac were pulse labeled with 3H-thymidine and chromosome samples were prepared for the next 32 h at 1--2 h intervals. A typical late replication pattern widely observed in heterochromatin was not found in the muntjac chromosomes. The following points make the DNA replication of the muntjac chromosomes characteristics: (1) Heterochromatin replicated its DNA in a shorter period with a higher rate than euchromatin. (2) Two small euchromatic regions adjacent to centromeric heterochromatin behaved differently from other portions of euchromatin, possessing shorter Ts, higher DNA synthetic rates and starting much later and ending earlier their DNA replication. (3) Segmental replication patterns were observed in the chromosomes 2 and 3 during the entire S phase. (4) Both homologues of the chromosome 3 showed a synchronous DNA replication pattern throughout the S phase except in the distal portion of the long arms during the mid-S phase.
mesh_terms: D000818:Animals; D001345:Autoradiography; D002478:Cells, Cultured; D002843:Chromatin / Q000648:ultrastructure; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure; D004261:DNA Replication; D003670:Deer / Q000235:genetics*; D006570:Heterochromatin / Q000648:ultrastructure; D007621:Karyotyping; D007700:Kinetics; D008297:Male; D012867:Skin / Q000648:ultrastructure
chemical_list: D002843:Chromatin; D006570:Heterochromatin