Rapid determination of glycine- and taurine-conjugated bile acids in human bile by high-performance liquid chromatography.
With use of an anion-exchange packing, TSK Gel IEX 540 DEAE, for high-performance liquid column chromatography, glycine- and taurine-conjugated bile acids were separated in 10 min and detected with a differential refractometer. Human bile could be analyzed after a simple pretreatment. The purity of the peaks of glycine- and taurine-conjugated bile acids in human bile was confirmed by enzymatic determinations using 3 alpha-hydroxysteroid:NAD oxidoreductase. The molar ratios of the two forms of the conjugates (glycine/taurine ratios) in bile from normal subjects and from patients suffering from various hepatobiliary diseases were measured.
D001646:Bile / Q000032:analysis*; D001647:Bile Acids and Salts / Q000032:analysis*; D002769:Cholelithiasis / Q000503:physiopathology; D002779:Cholestasis / Q000503:physiopathology; D002851:Chromatography, High Pressure Liquid; D003137:Common Bile Duct Diseases / Q000503:physiopathology; D003560:Cysts / Q000503:physiopathology; D005706:Gallbladder Neoplasms / Q000503:physiopathology; D005998:Glycine / Q000032:analysis*; D006801:Humans; D008722:Methods; D013654:Taurine / Q000032:analysis*
D001647:Bile Acids and Salts; D013654:Taurine; D005998:Glycine