Generation of a lymphocyte growth factor by treatment of human cells with neuraminidase and galactose oxidase.
Supernates of neuraminidase and galactose oxidase (NAGO)-treated lymphocytes induce blastogenesis in nonproliferating cells harvested 7--14 d after treatment with mitogen or alloantigen and in cells incubated with mitogen for 7--14 d but not in freshly isolated peripheral blood lymphocytes9 Virtually all the growth factor is produced by NAGO-treated cells during the first 24 h of incubation, and no increase in factor activity is detected upon further cell culture. Serum is not required for growth factor production. NAGO-primed medium induces generation of specific cytotoxic T cells from mixed lymphocyte culture (MLC) memory cells to approximately the same extent as that induced by allogeneic cells (stimulating cells in the primary MLC). NAGO-primed medium provides a useful reagent for isolation and characterization of lymphocyte growth factors and other lymphokines.
D000328:Adult; D002478:Cells, Cultured; D005692:Galactose Oxidase / Q000378:metabolism*; D006133:Growth Substances / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007156:Immunologic Memory; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000276:immunology*; D008222:Lymphokines / Q000302:isolation & purification; D008875:Middle Aged; D008934:Mitogens; D009439:Neuraminidase / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology
D006133:Growth Substances; D008222:Lymphokines; D008934:Mitogens; D010835:Phytohemagglutinins; D005692:Galactose Oxidase; D009439:Neuraminidase