Light scattering studies on alpha-crystallin from bovine eye lens.
Using the usual angular light scattering technique, we have measured the concentration dependence of reduced scattering intensity of fractionated bovine lens alpha-crystallin (alpha A-chain) in the presence of 30 mM calcium chloride and 0.1 M sodium chloride. The inverse intensity of scattered light at zero angle per unit concentration of alpha-crystallin has a significant upward curvature with increasing alpha-crystallin concentration. The third virial coefficient determined from this upward curvature was 1.24 x 10(-3) (mol 1/2 ml 1/2 g-1).
D000818:Animals; D002417:Cattle; D003459:Crystallins / Q000302:isolation & purification; D007908:Lens, Crystalline / Q000032:analysis*; D008027:Light; D046911:Macromolecular Substances; D008433:Mathematics; D011487:Protein Conformation; D012542:Scattering, Radiation
D003459:Crystallins; D046911:Macromolecular Substances