Secondary regulatory sites in rat liver cholesterol biosynthesis: role of 5-pyrophosphomevalonate decarboxylase.
The activity of 5-pyrophosphomevalonate decarboxylase in 43,000 g supernatant fractions from livers and kidneys of male adult rats has been determined. Enzyme activity in liver is significantly increased when rats are fed a diet containing 3% cholestyramine (268% of control rats) and decreased when fed a diet containing 2% cholesterol (25% of control rats). No circadian rhythm of enzyme activity is found in liver or kidneys. These results show that variations in hepatic cholesterogenesis affect the activity of 5-pyrophosphomevalonate decarboxylase in a similar way as other enzymes involved in the biosynthesis of cholesterol.
D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D002784:Cholesterol / Q000096:biosynthesis*; D002791:Cholesterol, Dietary / Q000494:pharmacology; D002792:Cholestyramine Resin / Q000494:pharmacology; D002940:Circadian Rhythm; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008798:Mevalonic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D009928:Organ Specificity; D051381:Rats
D002791:Cholesterol, Dietary; D002792:Cholestyramine Resin; D002784:Cholesterol; D002262:Carboxy-Lyases; C022503:pyrophosphomevalonate decarboxylase; D008798:Mevalonic Acid