A rapid and simple procedure for the preparation of human lactate dehydrogenase-2 from erythrocytes using an ion-exchange mini-column.
We describe a simple ion-exchange chromatography technique for the rapid preparation of lactate dehydrogenase-2 from lysed human erythrocytes obtained either from plasma or serum clots. The procedure appears capable of scale-up to larger columns thus making it ideal as an initial step in a more extensive purification scheme.
D002852:Chromatography, Ion Exchange / Q000379:methods*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood*
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase