title: Integration and organization of Ti plasmid sequences in crown gall tumors.
abstract: The Ti plasmid sequences (T DNA) maintained in four unorganized crown gall tumor lines were defined by using restriction endonucleases and molecular hybridization techniques. Each tumor line contains a "core" T DNA which is apparently responsible for maintaining the transformed state; the core T DNA is colinear with the Ti plasmid and contains the Ti plasmid sequences referred to as the "common DNA"--sequences found in all Ti plasmids studied to date. A given Ti plasmid does not always give rise to the same T DNA complement. The data suggest that the majority if not all of the T DNA is integrated into plant DNA, that preferred regions of the Ti plasmid serve as the points of attachment to plant DNA and that the T DNA can be linked to more than one site in the plant genome.
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010941:Plant Tumors / Q000382:microbiology; D010957:Plasmids; D011995:Recombination, Genetic; D012231:Rhizobium / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004247:DNA