Tumour-associated antigens in lung cancer: the possibility for a serologic assay.
Methods were developed to obtain a purified tumour-associated antigen from squamous cell bronchogenic carcinoma of humans. From the preparation a highly specific antitumour antiserum was produced in rabbits. The antiserum was applied to an enzyme-linked immunosorbent assay that subsequently was used as a serologic test for lung cancer. Serum obtained preoperatively from patients with stage I lung cancer was found to be inhibitory in the assay system when compared with serum from healthy individuals.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D002283:Carcinoma, Bronchogenic / Q000276:immunology*; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology* / Q000556:secondary; D011817:Rabbits
D000951:Antigens, Neoplasm