BAEE esterase (kallikrein-like) activity in parotid saliva of normal rats and rats with various forms of experimental hypertension (genuine, renovascular and DOCA salt hypertension).
A technique for continuous and quantitative collection of parotid saliva including salivary flow rate determination for in vitro experiments in rats is described. Kallikrein-like activity in parotid saliva of rats with various forms of arterial hypertension (genuine, renovascular and DOCA salt hypertension) was studied. Kallikrein-like excretion was measured by its estoerolytic activity using benzoyl arginine ethyl ester (BAEE) as a substrate. The levels of kallikrein-like activity in parotid saliva of normotensive control rats ranged between 2.5 and 4.0 mU/min during salivary flow stimulation with pilocarpine. In all forms of experimental hypertension kallikrein-like activity in saliva was increased two-to fourfold. This increase was not related to the activity of the renin-angiotensin system.
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003900:Desoxycorticosterone; D004195:Disease Models, Animal; D006973:Hypertension / Q000139:chemically induced / Q000201:enzymology*; D006977:Hypertension, Renal / Q000201:enzymology*; D006978:Hypertension, Renovascular / Q000201:enzymology*; D008297:Male; D010306:Parotid Gland / Q000201:enzymology*; D051381:Rats; D012463:Saliva / Q000201:enzymology*
D003900:Desoxycorticosterone; D001120:Arginine; D002265:Carboxylic Ester Hydrolases; C019763:benzoylarginine ethyl esterase