Determination of specific inhibitors of thromboxane A2 formation.
Activation of platelets by contact with collagen leads to the simultaneous formation of a prostaglandin and thromboxane (TXA2), which can be assayed biologically with the superfused rabbit aorta and rat stomach strip. By this method it has been shown that a cyclo-oxygenase inhibitor, aspirin, inhibited formation of both prostaglandin and TXA2 while a specific TXA2 synthetase inhibitor, clotrimazole, inhibited only TXA2 formation. The method is suitable as a primary screen in the search for TXA2 specific synthetase inhibitors.
D000125:Achilles Tendon / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000378:metabolism; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism; D003022:Clotrimazole / Q000494:pharmacology; D003864:Depression, Chemical; D005753:Gastric Mucosa / Q000378:metabolism; D066298:In Vitro Techniques; D011817:Rabbits; D013928:Thromboxane A2 / Q000096:biosynthesis*; D013931:Thromboxanes / Q000096:biosynthesis*
D013931:Thromboxanes; D013928:Thromboxane A2; D003022:Clotrimazole; D001241:Aspirin