Purification of a low molecular weight non-histone chromosomal protein.
A non-histone chromosomal proteins was extracted from rat liver chromatin with 0.35 M NaCl and purified more than 2758 times to near homogeneity by hydroxyapatite, gel filtration, and phosphocellulose chromatography. The final fraction was greater than 95% pure as judged by non-denaturing gel electrophoresis. The protein, designated loosely bound non-histone chromosomal protein 1, had an observed molecular weight of 15 700. This protein was demonstrated to increase the amount of RNA synthesized in a heterologous (Escherichia coli RNA polymerase) transcription system and, therefore, this activity was also used to monitor its purification. The availability of highly purified loosely bound non-histone chromosomal protein 1 will make possible an examination of its structural and/or functional role in chromatin.
D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation / Q000379:methods; D002843:Chromatin / Q000032:analysis*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification* / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D006882:Hydroxyapatites; D008099:Liver / Q000032:analysis*; D008297:Male; D008970:Molecular Weight; D012313:RNA / Q000096:biosynthesis; D051381:Rats
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006882:Hydroxyapatites; D012313:RNA; D004247:DNA; D012321:DNA-Directed RNA Polymerases