A protein, VPg, covalently linked to 36S calicivirus RNA.
Proteins associated with 36S virus RNA from Vero cells infected with San Miguel sea lion virus, type 2 (SMSV-2), were labelled with 125I. One protein, VPg, remained linked to RNA when subjected to deproteinization techniques. VPg labelled wtih 32P was observed on 36S RNA from purified virions; the quantity of label was compatible with two phosphates per genome. The estimated mol. wt. of SMSV-2 VPg was 15 000.
D000818:Animals; D002139:Caliciviridae / Q000032:analysis*; D002478:Cells, Cultured; D005591:Chemical Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009705:Nucleosides / Q000302:isolation & purification; D011489:Protein Denaturation; D012367:RNA, Viral / Q000032:analysis*; D046048:Seals, Earless; D014764:Viral Proteins / Q000032:analysis*; D014779:Virus Replication
D009705:Nucleosides; D012367:RNA, Viral; D014764:Viral Proteins