Incorporation of 5-bromodeoxyuridine into DNA in newborn rat tissues.
The incorporation of bromodeoxyuridine (BUdR) into newborn rat tissue DNA has been determined after i.p. injection of 5-bromo-2'-deoxy-[6-3H]uridine. Incorporation of the unchanged nucleoside was shown by hydrolysis and ion exchange chromatography of extracted DNA. In all tissues examined, more than 90% of the radioactivity incorporated was in the form of bromodeoxyuridine.
D000818:Animals; D000831:Animals, Newborn; D001973:Bromodeoxyuridine / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000378:metabolism*; D005260:Female; D005561:Formates; D008297:Male; D051381:Rats; D014018:Tissue Distribution
D005561:Formates; D004247:DNA; D001973:Bromodeoxyuridine