Lysis of erythrocytes as a result of microsomal lipid peroxidation induced by CCl4 or FeCl2.
Rat liver microsomal lipid peroxidation as measured by malondialdehyde formation induced by the NADPH-dependent metabolism of CCl4 led to a concomitant lysis of erythrocytes added to the incubation mixture. The hemolytic process was closely related to the rate of malondialdehyde formation. The time elapsed for initiation and completion of hemolysis and the rate of malondialdehyde formation correlated well with the concentration of CCl4 used. Similar results were obtained during FeCl2-induced microsomal lipid peroxidation and hemolysis. The occurrence of products with hemolytic activity during CCl4-induced microsomal lipid peroxidation is discussed in relation to the hepatotoxicity of CCl4.
D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology*; D005296:Ferrous Compounds / Q000494:pharmacology*; D006207:Half-Life; D006461:Hemolysis / Q000187:drug effects*; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology*; D007700:Kinetics; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats
D005296:Ferrous Compounds; D008054:Lipid Peroxides; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D007501:Iron