An improved method for the isolation of eosinophilic leukocytes from normal human blood.
A relatively short and simple method for the isolation of the eosinophils from normal human blood is reported. With a recovery of about 45-55%, cell preparations, showing a degree of purity of 90-98%, are obtained. The isolated cells are morphologically intact and viable, as assessed by the trypan blue exclusion test and by active phagocytosis.
D002469:Cell Separation / Q000379:methods*; D004804:Eosinophils; D006801:Humans; D010587:Phagocytosis