Semi-automated fluorometry of total estrogens in plasma during late pregnancy.
We describe a mechanized method for determining total estrogens in plasma during the last trimester of pregnancy. This method involves rapid enzymic hydrolysis, automatic extraction (in a tube) with cyclohexane/ethyl acetate, purification of the extracted material by anion-exchange chromatography on a disposable "mini-column" of Dowex AG1 X 2 (acetate form), and continuous-flow fluorometric quantification. Conditions of hydrolysis and extraction were studied. The mean within-day imprecision (CV) was 7% and between-day imprecision was 8.1%. Sensitivity was 75 nmol/L of plasma. Results are obtained in 4 h. The technique is quite suitable for routine determinations: 90 assays can be done by a team of three technicians in one working day.
D004967:Estrogens / Q000097:blood*; D005260:Female; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D006868:Hydrolysis; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D013997:Time Factors
D004967:Estrogens