The radiobiological response of the thyroid. Part I: Sheep thyroid cells in culture as a radiobiological model.
A system for culturing sheep thyroid cells in vitro is described. The properties of the cultures may be manipulated so that they preserve in vitro the follicular architecture typical of the thyroid, or so that they undertake cellular proliferation. The cultured cells also undertake iodide metabolism. These growth and differentiation characteristics can be preserved for at least three weeks, which is sufficient time for assay of many radiobiological end-points.
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured; D002999:Clone Cells; D003470:Culture Media; D007454:Iodides / Q000378:metabolism; D008854:Microscopy, Electron; D008954:Models, Biological; D012756:Sheep; D013961:Thyroid Gland / Q000378:metabolism / Q000528:radiation effects* / Q000648:ultrastructure; D013972:Thyrotropin; D013997:Time Factors
D003470:Culture Media; D007454:Iodides; D013972:Thyrotropin