title: Fractionation of large glycopeptides of human teratocarcinoma-derived cells by concanavalin A-Sepharose chromatography.
abstract: Human teratocarcinoma derived cells, line PA 1, were labeled with radioactive monosaccharides and subsequently digested with pronase. Large sized glycopeptides (fraction A) were isolated by gel filtration on Bio-Gel P-10. Their chromatography on concanavalin A-Sepharose gave three subfractions, two of which were eluted with a sugar-free buffer and the third with 10 mM alpha-methyl mannoside. The first subfraction (fraction A-Con A Ia) incorporated label from [3H]galactose and [3H]glucosamine and contained the largest components of fraction A. The second and the third subfractions (fractions A-Con A Ib and A-Con A II) were glycopeptides which incorporated label from tritiated fucose, mannose, galactose, and glucosamine. Even these molecules were of large size eluting partially at the void volume from Bio-Gel P-60. The glycopeptides of fraction A-Con A Ib contained mannose, fucose, galactose, N-acetylglucosamine, and N-acetylgalactosamine. Fucose and galactose residues occupied ultimate or penultimate positions at the nonreducing termini of the oligosaccharides. N-Acetylneuraminic acid, too, was present in the glycopeptides of fraction A.
mesh_terms: D002460:Cell Line; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003208:Concanavalin A; D006020:Glycopeptides / Q000302:isolation & purification*; D006801:Humans; D011402:Pronase; D013724:Teratoma / Q000378:metabolism*
chemical_list: D006020:Glycopeptides; D003208:Concanavalin A; D011402:Pronase