The nature of secretory agglutinins and aggregating factors. II. Biochemical and immunochemical properties of factors in human saliva and amniotic fluid.
Secretory conglutinin-like factor (SKF) and secretory bacterial aggregating factors (SBAF) of saliva and amniotic fluid were characterized as high molecular weight non-mucin glycoproteins. Most biochemical tests or procedures used in previous investigations did not permit an unequivocal distinction among immunoglobulins, non-mucin glycoprotein, or mucin factors. Partitioning with hot phenol enabled the separation of a high molecular weight non-mucin glycoprotein fraction from saliva and amniotic fluid with SKF and SBAF activity that was distinct from mucins and antibodies. Non-mucin glycoproteins, immunoglobulins and mucins represent three classes of secreted molecules capable of clumping bacteria.
D000328:Adult; D000373:Agglutinins / Q000276:immunology* / Q000302:isolation & purification; D000653:Amniotic Fluid / Q000276:immunology*; D005260:Female; D006023:Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007120:Immunochemistry; D007071:Immunoglobulin A, Secretory; D008297:Male; D009077:Mucins / Q000276:immunology; D011247:Pregnancy; D012463:Saliva / Q000276:immunology*; D013291:Streptococcus / Q000276:immunology
D000373:Agglutinins; D006023:Glycoproteins; D007071:Immunoglobulin A, Secretory; D009077:Mucins