[Effect of the addition of albumin on the preservation of hemoglobin solutions lyophilized in the presence of glucose].
In the case of the clinical use of hemoglobin solution, the possibility of storing this preparation in the lyophilized state will be very useful. Freeze-dried preparations containing glucose alone or associated with albumin (25. 50. 75. 100 g/1) have been stored under air in darkness at room temperature or + 4 degrees C. At room temperature, the study has been stopped after one year in consideration of the important oxidation and denaturation of hemoglobin. The samples stored at low temperature have been studied after two years. With glucose alone, freeze-dried hemoglobin has generally a better stability than the four albumin containing preparations in the different assays (methemoglobin and oxyhemoglobin levels, Hill number, shape of the Barcroft's curve). The p 50 and the oxyphoric capacity are always low. Our hypothesis of an enhancement of hemoglobin stability by albumin in presence of glucose is not confirmed; the denaturation is in correspondence with the albumin concentration.
D005612:Freeze Drying; D005947:Glucose; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D010108:Oxyhemoglobins / Q000378:metabolism; D012709:Serum Albumin; D012996:Solutions; D013696:Temperature
D006454:Hemoglobins; D010108:Oxyhemoglobins; D012709:Serum Albumin; D012996:Solutions; D005947:Glucose
Influence de l'addition d'albumine sur la conservation de la solution d'hémoglobine lyophilisée en présence de glucose.