[Induction of calcium conductivity of artificial lipid membranes by a glycoprotein isolated from mitochondria and a homogenate of bovine heart].
Glycoprotein (GP) inducing selective calcium transport through bilayer lipid membrane (BLM) from homogenate and mitochondria of beef heart was isolated by our method using no chaotrophic agents, detergents and proteolytic enzymes. 20-100 micrograms/ml of GP increase BLM, conductivity per 2-4 orders, producing potential close to the theoretical under ten fold gradient of calcium ions. M. W. of GP is about 40000 as determined by polyacrylamide gel electrophoresis in the presence of dodecyl sulfate. Thiol groups are revealed in GP essential for Ca2+-transporting activity.
D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D002352:Carrier Proteins / Q000302:isolation & purification*; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000302:isolation & purification; D008051:Lipid Bilayers; D008929:Mitochondria, Heart / Q000032:analysis; D009206:Myocardium / Q000032:analysis*
D002352:Carrier Proteins; D006023:Glycoproteins; D008051:Lipid Bilayers; D002118:Calcium
Induktsiia kal'tsievoĭ provodimosti i skusstvennykh lipidnykh membran glikoproteidom, vydelennym iz mitokhondriĭ i gomogenata serdtsa byka.