Failure of anti-inflammatory steroids to inhibit prostaglandin production by rat polymorphonuclear leucocytes.
1 Like rabbit polymorphonuclear (PMN) leucocytes, rat peritoneal glycogen-induced PMN leucocytes produced much greater amounts of prostaglandin when incubated with killed bacteria than in the absence of phagocytosable material. 2 Rat PMN leucocytes mainly prostaglandin E2 (PGE2), in amounts up to 17 ng/10(6) cells in 90 min incubation, some 25 times the amount produced by resting cells. 3 Indomethacin and meclofenamic acid inhibited prostaglandin production by resting and phagocytosing cells, the IC50 being of the order of 10(-6) to 10(-7) M for both drugs. 4 Hydrocortisone and dexamethasone at concentrations up to 10(-4) M did not cause significant dose-related inhibition of prostaglandin production in this system. 5 It is suggested that the phagocytosing PMN leucocyte is insensitive to the action of anti-inflammatory steroids with respect to prostaglandin production.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D010587:Phagocytosis; D011453:Prostaglandins / Q000096:biosynthesis*; D051381:Rats; D013997:Time Factors
D000893:Anti-Inflammatory Agents; D011453:Prostaglandins; D003907:Dexamethasone; D006854:Hydrocortisone