title: Compositional changes in soluble proteins of cerebral mantle, cerebellum, and brain stem of rat brain during development: a two-dimensional gel electrophoretic analysis.
abstract: Soluble proteins from the cerebral mantle, cerebellum, and brain stem of rat brains were analyzed at various developmental stages by a two-dimensional gel electrophoresis technique. The electrophoretic technique resolved the soluble proteins into 100--150 polypeptide spots on two-dimensional gels and gave reproducible and highly resolved profiles of them. Although most of major polypeptides were commonly found in all the three brain regions, some polypeptides were shown to be unique to a specific brain region. Each brain region was different in the electrophoretic profile of soluble proteins at every developmental stage examined, although there was considerable similarity in the profiles of each of the three brain regions in fetal animals (16--17 days), indicating that soluble proteins undergo different compositional changes in each of the three brain regions during postnatal development. In addition, the number of polypeptide spots on the electrophoretic profile increased remarkably during postnatal development in all of the three brain regions, suggesting that soluble proteins become more heterogeneous during postnatal development in each of the three brain regions.
mesh_terms: D000818:Animals; D001921:Brain / Q000254:growth & development*; D001933:Brain Stem / Q000032:analysis*; D002531:Cerebellum / Q000032:analysis*; D002540:Cerebral Cortex / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005333:Fetus / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D051381:Rats; D012995:Solubility
chemical_list: D009419:Nerve Tissue Proteins; D010455:Peptides