The kinetics of the incorporation of newly synthesized ribonucleic acid and protein into the ribosomes of the uterus of the oestrogen-stimulated immature rat.
The kinetics of the synthesis of the components of polyribosomes was investigated in the uterus of the immature rat responding to the administration of oestradiol-17 beta. The hormone brings about a rapid stimulation of the association of newly synthesized mRNA with uterine ribosomes, which is maximal 2-4 h after oestradiol administration and causes the aggregation of pre-existing ribosomes into polyribosomes. Despite the striking stimulation of rRNA synthesis 2-4 h after hormone treatment [Knowler & Smellie (1971) Biochem. J. 125, 605-614], the accumulation of new rRNA into ribosomes does not reach a peak until 12 h after administration. At this time, the incorporation of new ribosomal protein is also maximal. A second peak of incorporation of newly synthesized mRNA into polyribosomes follows the peak of ribosome synthesis and coincides with the oestrogen-activated synthesis of DNA.
D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D011132:Polyribosomes / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D051381:Rats; D012269:Ribosomal Proteins / Q000096:biosynthesis*; D014529:Uridine / Q000494:pharmacology; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D004958:Estradiol; D014529:Uridine