Transacylation as a chain-termination mechanism in fatty acid synthesis by mammalian fatty acid synthetase. Synthesis of butyrate and hexanoate by lactating cow mammary gland fatty acid synthetase.
1. Purified cow mammary gland fatty acid synthetase synthesized long-chain unesterified and short-chain esterified fatty acids. 2. A direct relationship was observed between the amount of short-chain products synthesized and the concentration of acetyl-CoA in the incubation medium. 3. The short-chain products were identified as butyryl-CoA and hexanoyl-CoA. 4. Inhibition of the terminating thioester hydrolase of the fatty acid synthetase complex with phenylmethanesulphonyl fluoride did not inhibit the synthesis of short-chain products. 5. It is suggested that the synthesis of short-chain fatty acids involves the reverse of the 'loading' reaction.
D000818:Animals; D002087:Butyrates / Q000378:metabolism*; D002208:Caproates / Q000378:metabolism*; D002417:Cattle; D003065:Coenzyme A / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005227:Fatty Acids / Q000096:biosynthesis; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis; D005260:Female; D007700:Kinetics; D007774:Lactation; D008321:Mammary Glands, Animal / Q000201:enzymology*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011247:Pregnancy; D014280:Triglycerides / Q000096:biosynthesis
D002087:Butyrates; D002208:Caproates; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D010664:Phenylmethylsulfonyl Fluoride; D064429:Fatty Acid Synthases; D003065:Coenzyme A