Rapid batch fractionation of ryegrass pollen allergens.
A rapid batch fractionation procedure employing ion-exchange matrices has been used to fractionate ryegrass pollen antigens and allergens. After sequential elution through CM-Sephadex and DEAE-Sephadex, a group of four electrophoretically distinct allergens with a molecular weight of approximately 32,000 was fractionated. These allergens correspond to the group I allergens previously isolated by Johnson and Marsh. This material may be suitable for clinical use where a characterised group I allergen preparation is required.
D000485:Allergens / Q000302:isolation & purification*; D000818:Animals; D001666:Binding Sites, Antibody; D002021:Buffers; D002417:Cattle; D005591:Chemical Fractionation / Q000379:methods; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004592:Electrophoresis, Starch Gel; D006801:Humans; D007122:Immunoelectrophoresis; D007073:Immunoglobulin E; D008970:Molecular Weight; D011058:Pollen; D011852:Radioallergosorbent Test; D012882:Skin Tests; D013997:Time Factors
D000485:Allergens; D002021:Buffers; D007073:Immunoglobulin E