Studies on the ultraviolet light sensitivity of Bloom's syndrome fibroblasts.
The sensitivity of Bloom's syndrome (bl/bl) fibroblasts to ultraviolet light (254 nm) has been estimated by 4 criteria: sister-chromatid exchange (SCE) formation, micronucleus production, cell survival, and host-cell reactivation of UV-irradiated adenovirus 2. In general, bl/bl strains did not differ significantly from the normal (+/+) strains in their response to UV treatment by any of the 4 criteria. One bl/bl strain, GM1492, was exceptional: It was abnormally sensitive to UV light in the SCE, micronucleus, and host-cell reactivation assays, but was not sensitive to UV as estimated by colony-forming ability. Thus, although one of the bl/bl strains studied in the experiments was sensitive to UV light as judged by some criteria, UV sensitivity is not a universal characteristic of Bloom's syndrome cells. It is unclear whether the UV sensitivity of the GM1492 strain reflects genetic diversity within the syndrome or some unrelated property of this strain.
D002460:Cell Line; D002467:Cell Nucleus / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002875:Chromosomes / Q000528:radiation effects*; D004392:Dwarfism / Q000235:genetics*; D005347:Fibroblasts / Q000528:radiation effects*; D006801:Humans; D008242:Lysogeny; D010787:Photosensitivity Disorders / Q000235:genetics*; D012854:Sister Chromatid Exchange; D012867:Skin; D013577:Syndrome; D014466:Ultraviolet Rays