An endothelial cell-derived growth factor.
Cell-free plasma-derived serum (PDS) is deficient in the platelet-derived growth factor and will not support the growth of 3T3 cells, fibroblasts, or smooth muscle cells. However, when PDS-containing medium is preincubated with endothelial cells, the medium becomes modified so that it will support growth. The activity produced by the endothelial cells results from a polypeptide of 10,000 to 30,000 daltons which has several features that differ from those of the platelet-derived growth factor, including heat instability and lack of adsorption to CM Sephadex.
D000818:Animals; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D004727:Endothelium / Q000166:cytology* / Q000378:metabolism; D006133:Growth Substances / Q000032:analysis / Q000096:biosynthesis*; D006358:Hot Temperature; D051379:Mice; D010455:Peptides / Q000032:analysis; D014357:Trypsin / Q000494:pharmacology
D003470:Culture Media; D006133:Growth Substances; D010455:Peptides; D014357:Trypsin