The effect of pH on enzyme-catalysed reactions: a computer program to determine pK values of enzyme ionisable groups from kinetic data.
The paper describes a non-linear multiple regression program to analyse the kinetic parameters Vm and Km as functions of pH, assuming that the activity of particular enzyme forms is determined by the ionisation of two groups, the active form of the enzyme being the half-ionised species.
D002384:Catalysis; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003201:Computers; D004798:Enzymes / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007477:Ions; D007700:Kinetics; D014534:UDPglucose 4-Epimerase / Q000378:metabolism
D004798:Enzymes; D007477:Ions; D014534:UDPglucose 4-Epimerase