Use of an alumina column in estimating total iron-binding capacity.
I describe a simple, rapid technique for saturating the transferrin in serum and then removing unbound (excess) iron. This technique involves use of an easily prepared column of basic chromatographic alumina and a saturating solution of ferric chloride in citric acid. This method, when compared with the magnesium carbonate method of Ramsay (Clin. Chim. Acta 2: 221, 1957) by regression analysis and tests of precision, showed a negative bias for results by the alumina technique with respect to the magnesium carbonate method.
D000537:Aluminum Oxide; D000818:Animals; D002417:Cattle; D002845:Chromatography / Q000379:methods; D006801:Humans; D007501:Iron / Q000097:blood*; D013053:Spectrophotometry; D014168:Transferrin / Q000032:analysis*
D014168:Transferrin; D007501:Iron; D000537:Aluminum Oxide