Uptake of 3H-demethylphalloin by isolated hepatocytes in the presence of various concentrations of phalloin or phalloidin.
The uptake of trace amounts of 3H-demethylphalloin (3H-DMP) by isolated hepatocytes was studied in the presence of various concentrations of unlabeled demethylphalloin (DMP), of phalloin and of phalloidin. The addition of phalloidin (or phalloin) reduces the uptake of 3H-DMP more than the addition of the equivalent concentration of DMP. The error caused by dilution of 3H-DMP with phalloin or phalloidin is not constant and depends on the concentration of the unlabeled compound. The relative differences between the uptake of 3H-DMP in the presence of demethylphalloin and in the presence of either phalloin or phalloidin cannot be explained by a competitive model. Some consequences for the use of 3H-DMP in toxicokinetic experiments are discussed.
D000470:Alkaloids / Q000378:metabolism*; D000818:Animals; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D010590:Phalloidine / Q000378:metabolism*; D051381:Rats; D013997:Time Factors
D000470:Alkaloids; D009842:Oligopeptides; D010590:Phalloidine; C000880:desmethylphalloin; C024287:phalloin