Structural studies on the murine Ia alloantigens. VII. Further evidence for two-gene control of the E/C alloantigens.
By utilizing comparative tryptic peptide analysis of the E/C alloantigens from additional H-2 recombinant strains, the gene encoding the E/C beta subunit was localized to the I-A or B subregions. By peptide mapping techniques, the E/C alpha- and beta-chains showed little structural homology with the A alloantigen alpha and beta subunits. In addition, comparisons of the E/C beta subunits from F1 strains (b x d) with those from both parental strains revealed that in a heterozygote the beta subunit from either allele (b or d) can associate with the alpha-chain from one allele (d). Thus, the formation of hybrid E/C antigens by cis-trans gene complementation increases the E/C alloantigen repertoire in heterozygotes.
D000595:Amino Acid Sequence; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D002874:Chromosome Mapping; D006183:H-2 Antigens; D007519:Isoantigens; D008214:Lymphocytes / Q000276:immunology; D008285:Major Histocompatibility Complex; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D010455:Peptides; D013154:Spleen / Q000166:cytology
D006183:H-2 Antigens; D007519:Isoantigens; D010455:Peptides