[Comparative study of the diagnostic value of disc electrophoresis of urinary proteins and measurement of the excretion of N-acetylglucosaminidase for the detection of renal tubule damage in chronic polyarthritis (author's transl)].
A method is described for the determination of the enzyme N-acetyl-beta-D-glucosaminidase in gel-filtered urine. The individual reaction parameters were tested, and the reliability of the method was determined. The stability of the enzyme in urine was investigated at different temperatures over a 6 week period. The excretion of N-acetyl-beta-D-glucosaminidase, like the analysis of urinary proteins by disc electrophoresis, is a sensitive parameter for renal damage. The two methods were compared, using 50 random urine samples from patients with chronic polyarthritis; both methods were found to have equal diagnostic value.
D000118:Acetylglucosaminidase / Q000652:urine*; D001168:Arthritis / Q000175:diagnosis* / Q000652:urine; D002908:Chronic Disease; D004796:Clinical Enzyme Tests; D004355:Drug Stability; D004589:Electrophoresis, Disc; D006596:Hexosaminidases / Q000652:urine*; D006801:Humans; D007684:Kidney Tubules / Q000473:pathology*; D011507:Proteinuria / Q000201:enzymology*; D013696:Temperature; D013997:Time Factors
D006596:Hexosaminidases; D000118:Acetylglucosaminidase
Vergleichende Untersuchung zur diagnostischen Wertigkeit von Diskelektrophorese der Urinoproteine und N-Acetylglucosaminidaseausscheidung zur Erkennung von tubulären Nierenschädigungen bei chronischer Polyarthritis.