Ribosomal protein modifications in liver injury: effect of carbon tetrachloride and extrahepatic cholestasis on protein phosphorylation.
The phosphorylation of ribosomal proteins in rat liver injured fro various times by carbon tetrachloride and extrahepatic cholestasis was studied. The incorporation of [32P]phosphate into the protein moiety of the small ribosomal subunit was increased over 2-fold 1 to 4 h after injection of the hepatotoxin, whereas the specific activity of the 60 S subunit was low and changed only insignificantly. Two-dimensional electrophoretic analysis of ribosomal proteins complemented by autoradiography revealed that CCl4 stimulated the phosphorylation of only a single protein (S6) in the 40 S subunit. The phosphorylation of S6 led to a measurable anodic shift and elongation of the protein on the 2-dimensional electropherograms. In response to bile duct ligation a similar increase in phosphorylation of protein S6 was noticed.
D000818:Animals; D001345:Autoradiography; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism*; D001651:Cholestasis, Extrahepatic / Q000378:metabolism*; D003135:Common Bile Duct / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D051381:Rats; D012269:Ribosomal Proteins / Q000378:metabolism*
D012269:Ribosomal Proteins