Intracellular glycosylation of influenza hemagglutinin: the effect of glucosamine.
The intracellular glycosylation of influenza virus hemagglutinin has been studied in calf kidney primary cultures infected with influenza virus A/NWS (H0N1). Intermediate glycosylated forms were obtained by the addition of glucosamine to the culture medium; they were characterized after [35S]-methionine labelling by their precursor relationship, by immunoprecipitation and peptide mapping. The underglycosylated hemagglutinins were distributed in classes of discrete molecular weight indicating that glycosylation occurred in a stepwise manner. A total of 8 different bands, suggesting 7 glycosylation sites, was obtained under conditions of both complete and incomplete glycosylation.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005944:Glucosamine / Q000494:pharmacology*; D006389:Hemagglutinins, Viral / Q000032:analysis; D009980:Influenza A virus / Q000187:drug effects / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis / Q000378:metabolism*
D006389:Hemagglutinins, Viral; D010455:Peptides; D014764:Viral Proteins; D005944:Glucosamine