title: Arginine synthesis and nitrogen excretion in the myxomycete Physarum polycephalum.
abstract: The nitrogen excretory metabolism of the myxomycete Physarum polycephalum was studied. When cultured in partially defined broth medium or on agar, the principal excretory product was ammonia nitrogen. A small, variable quantity of urea was excreted in liquid culture. No uric acid or other purines were detected in the cultures. When microplasmodia were incubated with sodium [14C]bicarbonate, radioisotope was incorporated into citrulline, arginine, and urea. Incubation with L-[carbamoyl-14C]citrulline yielded labelled arginine, urea, and CO2. Substantial urease activity was found in extracts of the microplasmodia. These results, in conjunction with the lack of an absolute nutritional requirement for arginine, provide evidence that Physarum has a functional arginine biosynthetic pathway, an arginase, and a urease.
mesh_terms: D000641:Ammonia / Q000378:metabolism; D001120:Arginine / Q000096:biosynthesis*; D002956:Citrulline / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D010804:Physarum / Q000378:metabolism*; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000378:metabolism; D014527:Uric Acid / Q000378:metabolism
chemical_list: D014527:Uric Acid; D002956:Citrulline; D000641:Ammonia; D014508:Urea; D001120:Arginine; D014510:Urease; D009584:Nitrogen