Immune mechanisms against canine distemper. III. Role of complement lysis in the immunity and persistent infection of canine distemper virus.
Antibody-mediated complement lysis of Vero cells and canine macrophages infected with canine distemper virus (CDV) was demonstratee in an in vitro 51Cr release assay. This cytolytic activity was found to be highly efficient and was optimal under conditions which favoured the capping of redistribution of surface viral antigens. A prozone was observed in the presence of high antibody concentration and could not be eliminated by repeated washings. By tagging antibody-coated target cells with 125I-labelled staphylococcus protein A, it was found that the extent of protein A binding was parallel to the degree of cytotoxicity suggesting that the mechanism of this prozone effect was similar to that of a precipitation test.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000920:Antibody-Dependent Cell Cytotoxicity; D002478:Cells, Cultured; D003165:Complement System Proteins / Q000276:immunology*; D004216:Distemper / Q000276:immunology*; D004217:Distemper Virus, Canine / Q000276:immunology; D004285:Dogs; D008264:Macrophages / Q000276:immunology; D013205:Staphylococcal Protein A / Q000276:immunology
D000914:Antibodies, Viral; D013205:Staphylococcal Protein A; D003165:Complement System Proteins