Separation of granulocytes from peripheral blood in a single step using discontinuous density gradients of Ficoll-Urografin. A comparative study with separation by dextran.
A single-step method for separating granulocytes from peripheral blood using two different gradients of Ficoll-Urografin (F-U) density 1.075 and 1.097 g/ml) is described. The morphological, cytochemical and ultrastructural properties of neutrophils isolated by this method were compared to those isolated by dextran-enhanced sedimentation and neutrophils from the peripheral blood. These studies indicated fewer alterations in neutrophils separated by F-U centrifugation than in neutrophils concentrated by dextran. Finally, the granulocyte layer recovered by F-U fractionation was 98% pure (dextran 71%) and yielded 64% (dextran 79%) of total granulocytes.
D000328:Adult; D002469:Cell Separation; D002499:Centrifugation, Density Gradient / Q000379:methods; D003911:Dextrans / Q000494:pharmacology*; D005260:Female; D005362:Ficoll / Q000494:pharmacology; D006098:Granulocytes; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000201:enzymology / Q000648:ultrastructure; D010544:Peroxidases
D003911:Dextrans; D005362:Ficoll; D010544:Peroxidases