title: Effect of membrane potential on Na+-dependent sugar transport by ATP-depleted intestinal cells.
abstract: The role of the membrane potential as a component of the thermodynamic driving force and as a determinant of kinetic parameters of Na+-dependent sugar transport was investigated using ATP-depleted isolated chicken intestinal cells. Inside-negative membrane potentials were established by incubating K+-loaded rotenone-inhibited cells with valinomycin in a low K+ medium. Overshoots of 3-O-methylglucose (3-OMG) accumulation as high as 10-fold were observed in the presence of valinomycin even in the absence of a Na+ chemical gradient. The magnitude of overshoot was diminished by decreasing the magnitude of the imposed K+ gradient and abolished altogether when nigericin was also included. An Eadie-Hofstee plot of initial flux data showed that the imposed membrane potential increases the Vmax of transport in the absence of a chemical gradient for Na+ from 3 to 12 nmol 3-OMG . mg protein-1 . min-1. The KT is not significantly altered. Similar kinetic results were obtained when a membrane potential as well as a Na+ gradient were imposed. These results suggest  that the membrane potential is a more important contributor to alterations in the kinetics of transport than the Na+ chemical potential.
mesh_terms: D000255:Adenosine Triphosphate / Q000172:deficiency; D000818:Animals; D001693:Biological Transport, Active; D002645:Chickens / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism / Q000494:pharmacology; D014634:Valinomycin / Q000494:pharmacology
chemical_list: D008757:Methylglucosides; D008759:Methylglycosides; D014634:Valinomycin; D000255:Adenosine Triphosphate; D012964:Sodium; D011188:Potassium