title: Immobilized-enzyme rate-determination method for glucose analysis.
abstract: We present a rate-determination method for analyzing glucose. A glucose enzyme electrode serves as the sensor and is made by placing a gel-immobilized layer of glucose oxidase over the tip of a Clark-type O2 electrode. The electrode membrane is made of Teflon and is derivatized by etching with a suspension of colloidal sodium metal in organic solvent. The enzyme is coupled to the membrane surface by use of paraformaldehyde. The immobilized-enzyme method is compared with a similar solution-enzyme method and with the National Glucose Reference method. The immobilized enzyme method compares favorably with the solution-enzyme method and offers the advantages of simplicity, economy of enzyme, and linearity over a greater range of concentration.
mesh_terms: D001205:Ascorbic Acid; D001663:Bilirubin; D001786:Blood Glucose / Q000032:analysis*; D003545:Cysteine; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D006454:Hemoglobins; D006593:Hexokinase; D006801:Humans; D008722:Methods; D012995:Solubility
chemical_list: D001786:Blood Glucose; D004800:Enzymes, Immobilized; D006454:Hemoglobins; D005949:Glucose Oxidase; D006593:Hexokinase; D003545:Cysteine; D001205:Ascorbic Acid; D001663:Bilirubin