A UGA termination suppression tRNATrp active in rabbit reticulocytes.
A tRNATrp was purified from rabbit reticulocytes which suppresses the UGA termination codon of beta-haemoglobin mRNA. Evidence is presented that the beta-haemoglobin readthrough protein is found in reticulocyte translations and intact cells. Some natural readthrough proteins perform essential functions; they are synthesised through suppression of UGA or UAG but not UAA termination codons.
D000818:Animals; D003062:Codon; D005914:Globins / Q000235:genetics; D008970:Molecular Weight; D010443:Peptide Chain Termination, Translational; D010446:Peptide Fragments / Q000032:analysis; D012333:RNA, Messenger / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*; D013489:Suppression, Genetic; D014364:Tryptophan
D003062:Codon; D010446:Peptide Fragments; D012333:RNA, Messenger; D014364:Tryptophan; D005914:Globins; D012343:RNA, Transfer