title: Characterization of immune responses of cattle to erythrocyte stroma, Anaplasma antigen, and dodecanoic acid-conjugated Anaplasma antigen: cell-mediated immunity.
abstract: Sonically disrupted normal erythrocyte stroma (SES) and two anaplasma antigens (sonically disrupted anaplasma antigen; SAA, and French pressure cell disrupted anaplasma antigen; FAA) were prepared from normal and Anaplasma marginale-infected blood. The SAA and FAA antigens were chemically modified by conjugation with dodecanoic acid (SAADA and FAADA). Significant (P less than or equal to 0.05) anti-anaplasma lymphocyte-transformation responses were obtained from all cattle given SAA, SAADA, or FAADA vaccines. Only cows given SAA developed anti-anaplasma antibody. Mild antierythrocyte lymphocyte-transformation responses were obtained from most vaccinated animals. Delayed hypersensitivity to erythrocyte antigen was not detected. The SAA-vaccinated cows had the highest degree of protection in that they developed a smaller percentage of parasitemia and had less severe anemia than did other cattle in the study. The SAADA- and FAADA-vaccinated cattle developed a good cell-mediated immune response, but poor humoral immune response and had lower parasitemias than did challenge-exposed controls; but they developed severe anemia. It is suggested that cellular and humoral mechanisms are essential for protection in anaplasmosis. Evidence of protection from clinical anaplasmosis was not observed in SES-vaccinated cows.
mesh_terms: D000709:Anaplasma / Q000276:immunology*; D000712:Anaplasmosis / Q000517:prevention & control; D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D002418:Cattle Diseases / Q000517:prevention & control; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D007111:Immunity, Cellular; D007850:Lauric Acids / Q000494:pharmacology*; D008213:Lymphocyte Activation; D012882:Skin Tests; D014611:Vaccination / Q000662:veterinary
chemical_list: D000942:Antigens, Bacterial; D007850:Lauric Acids