[Isolation, purification and properties of acid ribonuclease from the lysosomal fraction of silkworm eggs].
Acid ribonuclease (ribonucleate-3'-oligonucleotide hydrolase, EC 3.1.4.23) has been isolated from the lysosomal fraction of Bombyx mori eggs. The enzyme has a pH optimum of 4,7 and a molecular weight of 17 000 +/- 1000; the isoelectric point of the enzyme lies around 6,0. The enzyme splits RNA and poly(U) down to nucleoside-3'-phosphates to form intermediates--nucleoside-2',3'-cyclophosphates. Polyadenylic acid is hydrolyzed in the presence of the enzyme down to oligonucleotides. Mg2+ suppress the enzyme activity.
D000818:Animals; D012831:Bombyx / Q000201:enzymology*; D005260:Female; D007700:Kinetics; D008247:Lysosomes / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D010063:Ovum / Q000201:enzymology*; D011061:Poly A; D012260:Ribonucleases / Q000302:isolation & purification / Q000378:metabolism*
D011061:Poly A; D012260:Ribonucleases; D008274:Magnesium
Vydelenie, ochistka i svoĭstva kisloĭ ribonuckleazy lizosomal'noĭ fraktsii greny tutovogo shelkopriada.