[Multiphasic character of the kinetics of cytochrome P-450 destruction in microsomal LM2- and LM4-forms in the reaction with cumene hydroperoxide].
Cytochrome P-450 destruction kinetics by cumene hydroperoxide has been studied in LM2 and LM4 microsomal and purified forms. Three destruction phases of cytochrome P-450 were shown to be observed irrespective of the source and integration degree, cytochrome P-450 pseudomonomolecular consumption rate constants being dependent in a complex way upon the cumene hydroperoxide initial concentration. The radical character of cytochrome P-450 destruction was proved by experiments with 1-naphtol. The mechanism of radicals formation is discussed.
D000818:Animals; D001555:Benzene Derivatives; D055598:Chemical Phenomena; D002621:Chemistry; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010545:Peroxides; D010634:Phenobarbital / Q000494:pharmacology; D011817:Rabbits; D051381:Rats
D001555:Benzene Derivatives; D010545:Peroxides; D003577:Cytochrome P-450 Enzyme System; C007164:cumene hydroperoxide; D010634:Phenobarbital
Mnogofaznyĭ kharakter kinetiki destruktsii tsitokhroma P-450 v mikrosomal'noĭ LM2- i LM4-formakh v reaktsii s gidroperekis'iu kumila.