Intermediate filaments of bovine pulmonary artery smooth muscle distribution, isolation and polypeptide composition.
1. The distribution of intermediate filaments in bovine pulmonary artery smooth muscle was studied. 2. These filaments contained at least two polypeptides of 43,000 and 55,000 daltons. 3. The 55,000 dalton polypeptide was purified by ion-exchange chromatography. 4. The amino acid compositions of the polypeptides were determined. 5. The 55,000 dalton polypeptide and a mixture of the 43,000 and 55,000 dalton polypeptide reassembled into structures similar to native intermediate filaments.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000032:analysis* / Q000648:ultrastructure*; D009940:Organoids / Q000032:analysis; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D011651:Pulmonary Artery / Q000032:analysis
D000596:Amino Acids; D010455:Peptides