Stability of DNA/anti-DNA complexes. III. Kinetic control of immune complex size.
The Farr assay and sucrose gradient ultracentrifugation were used to study the importance of kinetic factors on the size of antibody/DNA immune complexes prepared from SLE sera and dsDNA. We have found that significantly different numbers and sizes of antibody/DNA immune complexes can be formed by varying the time course of addition of a given amount of DNA to an SLE serum. For example, the introduction of additional DNA to a preincubated antibody-DNA system does not lead to reequilibration of that system with respect to the amount of DNA bound or the size of the complexes formed even after 1 hr at 37 degrees C. The potential implications of these observations with respect to the pathogenesis of SLE is discussed.
D000915:Antibody Affinity; D000936:Antigen-Antibody Complex; D001666:Binding Sites, Antibody; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000276:immunology*; D006801:Humans; D007185:Incubators; D007700:Kinetics; D013997:Time Factors
D000936:Antigen-Antibody Complex; D004247:DNA