The fractionation of suspensions of isolated hepatocytes by rate zonal centrifugation.
Parenchymal cells, isolated from rat liver by a simple non-enzymic technique, were fractioned according to ploidy class by rate zonal centrifugation on sucrose density gradients. This method of fractionation applied to liver cells prelabelled in vivo with tritiated thymidine separated different size classes of cells synthesising DNA.
D000375:Aging; D000818:Animals; D001835:Body Weight; D002452:Cell Count; D002467:Cell Nucleus; D002469:Cell Separation / Q000379:methods; D002501:Centrifugation, Zonal / Q000379:methods*; D004247:DNA / Q000096:biosynthesis*; D005260:Female; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D011003:Ploidies; D051381:Rats; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D013936:Thymidine