Removal of plasma lipoproteins from circulating blood with a heparin-agarose column.
A method is described for the removal of cholesterol fractions by means of intermittent extracorporeal plasma circulation through a column containing heparin-treated agarose in the presence of calcium. The system allows plasma perfusion in one column while simultaneously recharging another. In vitro testing demonstrated its efficiency, and in vivo testing of a normal dog proved its feasibility. In vitro testing revealed a mean removal of 22% of low-density and very low-density lipoproteins combined. Animal testing did not produce any abnormalities in clinically significant parameters.
D000818:Animals; D002784:Cholesterol / Q000097:blood; D004285:Dogs; D006464:Hemoperfusion / Q000379:methods*; D006493:Heparin; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000628:therapy; D066298:In Vitro Techniques; D008074:Lipoproteins / Q000097:blood*; D010956:Plasmapheresis; D012685:Sepharose
D008074:Lipoproteins; D006493:Heparin; D012685:Sepharose; D002784:Cholesterol