A hybridoma from an autoimmune NZB/NZW mouse producing monoclonal antibody to ribosomal-RNA.
Spleen cells from an unimmunized autoimmune NZB/NZW F1 female mouse were fused to the BALB/c MPC-11 drug-resistant clone 45.6 TG 1.7. One hybrid clone (D4) produced IgG-3 immunoglobulin that bound ribosomal RNA. A high concentration of this antibody was produced in the ascitic fluid of NZB/NZW male mice. DNA, tRNA, and synthetic single- and double-stranded polynucleotides could not bind significantly to the antibody. A Scatchard analysis showed that the rRNA-binding immunoglobulin is monoclonal and has a high affinity (10(9) liter/mole) for the RNA antigen.
D000818:Animals; D000917:Antibody Formation; D001202:Ascitic Fluid / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology*; D001666:Binding Sites, Antibody; D002459:Cell Fusion; D002999:Clone Cells / Q000276:immunology; D005260:Female; D006041:Goats; D006822:Hybrid Cells / Q000276:immunology*; D008297:Male; D051379:Mice; D008814:Mice, Inbred NZB; D012335:RNA, Ribosomal / Q000276:immunology*; D013154:Spleen / Q000276:immunology
D012335:RNA, Ribosomal