Effects of SCN- and NO3- on organic anion transport in rabbit kidney cortical slices.
1. The effects of replacement of Cl- by either SCN- or NO3- on the accumulation of p-aminohippurate, the efflux of pre-accumulated p-amino-hippurate and kinetics of p-aminohippurate uptake were investigated in the rabbit kidney cortical slice. 2. The total replacement of Cl- in the incubation medium with SCN- decreased the 60-min slice-to-medium concentration ratio (S/M) of p-amino-hippurate by 75% and that with NO3- by 40%. 3. The decrease in p-aminohippurate accumulation by the inorganic ions was found to be specific for organic anion transport since the uptake of the organic cation, tetraethylammonium, was not influenced by inorganic ions. 4. The influence of NO3- on p-aminohippurate uptake was fully reversible: however, the effect of SCN- could only be partially reversed. 5. Both SCN- and NO3- significantly increased the Km value but had no significant effect on the V value of the p-aminohippurate uptake process. 6. These findings suggest that both SCN- and NO3- are competitive inhibitors of p-aminohippurate transport and, also, that SCN- appears to bind to a membrane component involved in the transport of p-aminohippurate.
D000618:Aminohippuric Acids / Q000378:metabolism*; D000818:Animals; D000838:Anions; D001692:Biological Transport / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D009566:Nitrates / Q000494:pharmacology*; D011817:Rabbits; D013861:Thiocyanates / Q000494:pharmacology*; D010130:p-Aminohippuric Acid / Q000378:metabolism*
D000618:Aminohippuric Acids; D000838:Anions; D002712:Chlorides; D009566:Nitrates; D013861:Thiocyanates; D010130:p-Aminohippuric Acid