Enhancement of the growth of blood monocytes in agar cultures by haemolysates.
Mouse blood monocytes grow well in liquid but poorly in agar medium. In this study the effect of haemolysate on the clonal growth of blood monocytes in agar cultures was investigated. The results suggested that haemolysate from mouse or rat red blood cells contains a factor(s) that can render the murine blood monocytes able to grow well in agar culture (anchorage-independent growth). The mechanism of action of the enhancing factor(s) is discussed.
D000818:Animals; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004912:Erythrocytes; D051379:Mice; D009000:Monocytes / Q000378:metabolism*; D013997:Time Factors
D003470:Culture Media