Liposome-cell interactions. A rapid assay for cells in suspension culture.
A method has been developed for the rapid separation of cells in suspension from non-cell associated lipid vesicles in various assays for vesicle-cell interation. Separation is achieved on a discontinuous Ficoll-Paque gradient. Cells and free vesicles are totally separated, as evidenced by both radiolabelled vesicles, and vesicles containing the fluorescent dye 6-carboxyfluorescein. The main advantages of this method are the rapidity, efficacy, and gentleness of the separation. Viability of the cells remains consistently high (greater than 96%) throughout the separation. Since this method involves a one-step centrifugation, it precludes the necessity for repeated washings of cells which have been incubated with lipid vesicles.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D007939:Leukemia L1210 / Q000473:pathology*; D008081:Liposomes; D051379:Mice; D010713:Phosphatidylcholines
D008081:Liposomes; D010713:Phosphatidylcholines