Purification of Giardia muris trophozoites by using nylon fiber columns.
A method is described for purification of large quantities of Giardia muris trophozoites from the small intestine of the mouse by using density layer centrifugation and nylon fiber columns. Centrifugation of trophozoites obtained from the small intestine on Metrizamide, specific gravity 1.10, followed by incubation on a nylon fiber column at 37 degrees C for 120 min, yielded up to 15 X 10(6) viable purified trophozoites per infected mouse.
D000818:Animals; D002499:Centrifugation, Density Gradient / Q000379:methods*; D005872:Giardia / Q000302:isolation & purification*; D007421:Intestine, Small / Q000469:parasitology; D007814:Larva; D051379:Mice; D009757:Nylons; D013696:Temperature
D009757:Nylons