title: Characterization of a unique population of unfilled estrogen-binding sites associated with the nuclear fraction of immature rat uteri.
abstract: Studies were initiated to determine whether resident nuclear estrogen receptors with unfilled binding sites existed in the nuclear fraction of uteri from untreated immature rats. These studies revealed a small population (mean +/- SEM, 0.087 +/- 0.017 pmol/uterus) of unfilled estrogen-binding sites in the uterine nuclear fraction from untreated immature rats, which bound [3H]estradiol in the absence of added cytoplasmic receptor. These nuclear estrogen-binding sites occurred in addition to the 0.60--1.2 pmol estrogen-binding activity/uterus in the uterine cytoplasmic fraction of these animals. [3H]Estradiol bound reversibly with high affinity (apparent Kd = 1.4 x 10(-10)M to these binding sites, and only estrogenic compounds competed for this binding. Binding studies done at a variety of temperatures (0--37 C) showed that there were no estradiol-filled receptor sites associated with the nuclear fraction. In addition, these unfilled nuclear estrogen-binding sites remained unfilled 15 min after injections of either 1.0 or 0.1 micrograms estradiol/rat. Extraction of these sites was achieved with 0.4 M KCl, and when this extract was centrifuged on a 5--20% linear 0.010 M Tris-HCl, 0.0015 M Na2EDTA, and 0.40, M KCl sucrose gradient, the binding activity exhibited a sedimentation coefficient of 3.6S. These unfilled nuclear estrogen-binding sites did not appear to have arisen as a contaminant from the estrogen-binding proteins present in either uterine cytoplasm or serum during tissue homogenization. The existence of these unfilled nuclear estrogen-binding sites does not represent a major exception to the classical two-step theory of estrogen action; instead, they seem to be novel forms of the estrogen receptor whose physiological role has not been determined.
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002467:Cell Nucleus / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D004958:Estradiol / Q000378:metabolism; D005260:Female; D007700:Kinetics; D051381:Rats; D011960:Receptors, Estrogen / Q000187:drug effects / Q000378:metabolism*; D012741:Sexual Maturation; D014599:Uterus / Q000378:metabolism*
chemical_list: D003902:Detergents; D011960:Receptors, Estrogen; D004958:Estradiol