title: Ribosomal protein modifications in liver injury: effect of carbon tetrachloride and extrahepatic cholestasis on protein phosphorylation.
abstract: The phosphorylation of ribosomal proteins in rat liver injured fro various times by carbon tetrachloride and extrahepatic cholestasis was studied. The incorporation of [32P]phosphate into the protein moiety of the small ribosomal subunit was increased over 2-fold 1 to 4 h after injection of the hepatotoxin, whereas the specific activity of the 60 S subunit was low and changed only insignificantly. Two-dimensional electrophoretic analysis of ribosomal proteins complemented by autoradiography revealed that CCl4 stimulated the phosphorylation of only a single protein (S6) in the 40 S subunit. The phosphorylation of S6 led to a measurable anodic shift and elongation of the protein on the 2-dimensional electropherograms. In response to bile duct ligation a similar increase in phosphorylation of protein S6 was noticed.
mesh_terms: D000818:Animals; D001345:Autoradiography; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism*; D001651:Cholestasis, Extrahepatic / Q000378:metabolism*; D003135:Common Bile Duct / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000378:metabolism*; D008297:Male; D010766:Phosphorylation; D051381:Rats; D012269:Ribosomal Proteins / Q000378:metabolism*
chemical_list: D012269:Ribosomal Proteins