title: Effect of methotrexate concentration and exposure time on mammalian cell survival in vitro.
abstract: Chinese hamster, HeLa and HAK cells were treated with methotrexate (MTX) to determine the dependence of its effect on drug concentration and exposure time. With a broad range of survival curves for Chinese hamster cells, cell survival is an exponential function of exposure time and a power function of drug concentration. The data allow a mathematical description to be made of the interdependence of MTX concentration and drug exposure in relation to cell survival.
mesh_terms: D000328:Adult; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D006367:HeLa Cells; D006801:Humans; D007668:Kidney / Q000166:cytology; D008727:Methotrexate / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D008727:Methotrexate