Lysis of rat liver lysosomes through incubation with 1-leucyl-glycine, substrate for a lysosomal dipeptidase.
1-Leu-Gly is a substrate for a lysosomal dipeptidase. The incubation of subcellular fractions in the presence of the dipeptide causes a rapid lysis of the particles, as revealed by the increase of both free and soluble activities of acid phosphatase. This effect is due to the hydrolysis of the dipeptide within the particles, as documented by the concomitant aminoacid production. The enhancing effect of EDTA on this phenomenon is reported.
D000135:Acid Phosphatase / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D004150:Dipeptidases / Q000378:metabolism; D004151:Dipeptides / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D051381:Rats
D000596:Amino Acids; D004151:Dipeptides; C015905:glycylleucine; D000135:Acid Phosphatase; D004150:Dipeptidases