title: Influence of dietary portein on the disposition and metabolism of phenylbutazone in rats.
abstract: Information of dietary protein on the disposition and metabolism of phenylbutazone was investigated in male rats fed ad libitum A 21% (control) or a 5% (low) protein diet for 3 weeks. Phenylbutazone and its metabolites were assayed by high-pressure liquid chromatography. Dietary protein deficiency was associated with a decrease in the conversion of phenylbutazone into oxyphenbutazone by 9000 x g liver supernatant of protein-deficient rats. Also, dietary protein deficiency was associated with a decrease in the urinary excretion of various metabolites of phenylbutazone, namely, oxyphenbutazone, gamma-hydroxyphenylbutazone, beta-hydroxyphenylbutazone, p,gamma-dihydroxyphenylbutazone, and an unknown metabolite (not identified). Pretreatment with various metabolites in both groups of animals. Within 5 min after an injection of phenylbutazone, plasma contained oxyphenbutazone; the area under the curve of oxyphenbutazone was significantly greater in protein-deficient rats than in controls possibly due to a greater accumulation. It is concluded that dietary protein deficiency is associated with a decrease in the disposition and metabolism of phenylbutazone in rats.
mesh_terms: D000818:Animals; D001711:Biotransformation; D004044:Dietary Proteins; D006207:Half-Life; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D008297:Male; D010113:Oxyphenbutazone / Q000097:blood; D010653:Phenylbutazone / Q000378:metabolism* / Q000652:urine; D011488:Protein Deficiency / Q000378:metabolism*; D051381:Rats
chemical_list: D004044:Dietary Proteins; D010653:Phenylbutazone; D010113:Oxyphenbutazone