An improved method for isolation of Ca2+ binding cyanogen bromide peptides from rabbit skeletal muscle troponin C.
A cyanogen bromide digest of troponin C from rabbit skeletal muscle yields two biologically active Ca2+ binding peptides. A simple, one-step method for the simultaneous purification, in high yield, of these two peptides is presented.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002135:Calcium-Binding Proteins / Q000302:isolation & purification*; D055598:Chemical Phenomena; D002621:Chemistry; D003488:Cyanogen Bromide; D009124:Muscle Proteins / Q000032:analysis*; D011817:Rabbits; D014336:Troponin / Q000032:analysis*; D019209:Troponin C
D000596:Amino Acids; D002135:Calcium-Binding Proteins; D009124:Muscle Proteins; D014336:Troponin; D019209:Troponin C; D003488:Cyanogen Bromide