The effect of troxypyrrolidinium, a choline uptake inhibitor, on the excitatory innervation of the rat urinary bladder.
Troxypyrrolidinium, a choline uptake inhibitor, reduced but failed to abolish responses of the rat urinary bladder to electrical stimulation at 1-100 Hz although it reduced acetylcholine output during stimulation at 10 Hz to a level similar to that of spontaneous release. Inhibition of the response to stimulation was more complete at faster rates of stimulation and was partially reversed by choline. Troxypyrrolidinium produced a greater inhibition of the 'tonic' component of the response to electrical stimulation than of the 'phasic' component. Hemicholinium-3 or hyoscine produced a similar selective effect on the 'tonic' component. Hemicholinium-3 also reduced acetylcholine output during electrical stimulation to a similar extent as troxypyrrolidinium but hyoscine increased transmitter output. The results support the concept of a second transmitter in the excitatory innervation of the bladder.
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D002794:Choline / Q000037:antagonists & inhibitors*; D004347:Drug Interactions; D004558:Electric Stimulation; D006426:Hemicholinium 3 / Q000494:pharmacology; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D011759:Pyrrolidines / Q000494:pharmacology*; D051381:Rats; D012601:Scopolamine / Q000494:pharmacology; D013997:Time Factors; D001743:Urinary Bladder / Q000187:drug effects* / Q000294:innervation / Q000502:physiology
D011759:Pyrrolidines; D006426:Hemicholinium 3; C005867:troxypyrrolium tosylate; D012601:Scopolamine; D002794:Choline; D000109:Acetylcholine