Matrix algebraic simulation of mitotic cell selection experiments.
Mitotic cell selection experiments are frequently utilized in investigations on the effects of various metabolic inhibitors and/or X-irradiation on cell-cycle progression of mammalian cells in tissue culture. This study describes a method for matrix algebraic simulation of these experiments which involves the sequential multiplication of cell-population matrices, growth matrices, and mitotic cell-selection matrices. Simulations are shown for progression in control cultures and cultures perturbed either by X-rays or 2-mercapto-1(beta-4-pyridethyl) benzimidazole. Application of this model will enhance the planning of these investigations and allow more rigorous testing of alternate hypotheses concerning the mechanisms of action of perturbing agents.
D000818:Animals; D001562:Benzimidazoles / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D002469:Cell Separation; D003201:Computers; D006224:Cricetinae; D005260:Female; D008433:Mathematics; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects; D008954:Models, Biological; D010053:Ovary; D011725:Pyridines / Q000494:pharmacology; D014965:X-Rays
D001562:Benzimidazoles; D011725:Pyridines; C005770:2-mercapto-1-(beta-4-pyridethyl)benzimidazole