The cellular nature of concanavalin A-stimulated antibody-dependent cell-mediated cytotoxicity.
Enhanced antibody-dependent cytotoxic cell (ADCC) activity, generated in vitro by concanavalin A (Con A) stimulation of human peripheral blood lymphocytes, was analyzed to identify the nature of the killer (K) cell. Stimulated and unstimulated 2-day cultured cells were separated into sheep erythrocyte rosetting (E+) and non-rosetting (E-) subpopulations, and ADCC activity was determined by using the slopes of linear dose-response plots as a measure of K cell activity. Quantitative comparisons were made by examining activity on a per cell basis ("specific" activity) and total K cell activity recovered per subpopulation. This analysis revealed that although the E- cells were enriched for specific ADCC activity, the E+ subpopulation contained the major proportion of the total recovered activity and nearly all of the Con A-induced increase in K cell activity.
D000920:Antibody-Dependent Cell Cytotoxicity; D003208:Concanavalin A / Q000494:pharmacology*; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007694:Killer Cells, Natural / Q000276:immunology; D008766:Methylmannosides / Q000494:pharmacology; D012397:Rosette Formation; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology
D008766:Methylmannosides; D003208:Concanavalin A; D000338:beta-Aminoethyl Isothiourea