[Various properties of partially purified dexamethasone receptor complexes of the rat liver].
A 300-fold purification of glucocorticosteroid-receptor complexes (GRC) of the rat liver with the 10--25% yield was conducted by combining the methods of binding with DNA-cellulose and salting out with ammonium sulfate at 30% saturation. An important feature of this method was a preponderant removal of protein admixtures capable of dissociation with DNA. In comparison of column chromatography and batch-procedure the latter proved to possess a number of advantages. Some properties of partially purified GRC were also studied. As revealed, the purified GRC which rapidly decomposed at 2 degrees C, could be partially stabilized by addition of inert protein, glycerine, SH-reagents and an excess of dexamethazone. Under such conditions about 13% of the initial amount of purified GRC was preserved after a week of storage at 2 degrees C. Determination of purified GRC content in the preparation by sorption on dextran coated carbon gave sharply diminished results; as to the method of oxyapatite sorption--it proved to be adequate. In comparing the GRC properties without and after the purification demonstrated that sedimentation and other properties of these preparations largely coincided. GRC-acceptor capacity of one- and two-chain DNA depended to a different degree on cytoplasmic admixtures in the GRC preparation removed in its purification.
D000818:Animals; D002482:Cellulose / Q000494:pharmacology; D002499:Centrifugation, Density Gradient / Q000379:methods; D003600:Cytosol / Q000032:analysis*; D004247:DNA / Q000494:pharmacology; D003907:Dexamethasone / Q000032:analysis* / Q000302:isolation & purification; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000032:analysis* / Q000302:isolation & purification; D011987:Receptors, Steroid / Q000032:analysis*
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003907:Dexamethasone; D002482:Cellulose; D004247:DNA
Nekotorye svoĭstva chastichno ochishchennykh deksametazon-retseptornykh kompleksov pecheni krys.