Successful culture of rat embryos on human serum: use in the detection of teratogens.
Growth of head-fold-stage rat embryos cultured with human serum for 48 hours was enhanced by supplementation with glucose. Embryo growth (protein and DNA contents) varied with the source of the serum. Serum from 16 of 19 untreated subjects produced normal embryos. Serum from five subjects undergoing cancer chemotherapy and six subjects receiving anticonvulsants was either lethal or teratogenic.
D000328:Adult; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D001769:Blood; D003470:Culture Media; D046508:Culture Techniques; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004622:Embryo, Mammalian / Q000187:drug effects; D005260:Female; D005947:Glucose; D006801:Humans; D008297:Male; D051381:Rats; D013723:Teratogens
D000927:Anticonvulsants; D000970:Antineoplastic Agents; D003470:Culture Media; D013723:Teratogens; D005947:Glucose