Unequal alpha and beta globin mRNA in reticulocytes of normal and mutant f/f fetal mice.
Messenger RNA (mRNA) was isolated from adult as well as normal and mutant f/f fetal mouse reticulocytes by poly-U affinity chromatography. mRNA from normal adult reticulocytes directed equal synthesis of alpha and beta globin chains in wheat germ cell free translational system. mRNA from either normal or mutant f/f fetal reticulocytes directed 40% more alpha globin synthesis than beta globin synthesis. These observations are suggestive that there is significantly more alpha mRNA than beta mRNA in normal and mutant f/f fetal mouse reticulocytes.
D000818:Animals; D005312:Fetal Blood / Q000378:metabolism*; D005914:Globins / Q000096:biosynthesis*; D051379:Mice; D008817:Mice, Mutant Strains; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000097:blood*; D012156:Reticulocytes / Q000378:metabolism*
D012333:RNA, Messenger; D005914:Globins