Glucuronide and sulphate binding to subcellular fractions of rat liver.
The binding of glucuronides to various liver subfractions and to bovine serum albumin correlated with their molecular weight. Higher molecular weight glucuronides bound more readily. 4-Methylumbelliferylglucuronide has some of the characteristics of phenolphthaleinglucuronide. Simple phenolic glucuronides were much less retained by various hepatic fractions. Glucuronides were most effectively bound to mitochondrial subfractions. Sulphates of 4-nitrophenol and phenolphthalein were bound to the same degree to bovine serum albumin and hepatic cytosol despite their different molecular structures and weights.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism; D008970:Molecular Weight; D051381:Rats; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013464:Sulfuric Acids / Q000378:metabolism*
D005965:Glucuronates; D013464:Sulfuric Acids; D012710:Serum Albumin, Bovine