Amniotic fluid phosphatidylglycerol and phosphatidylinositol separated by stepwise-development thin-layer chromatography.
We describe a stepwise-development thin-layer-chromatographic technique for separating phosphatidylglycerol and phosphatidylinositol in samples of amniotic fluid. Plates are prepared from silica gel G slurried in a 50 g/L ammonium sulfate solution. Phospholipid phosphorus determination is not needed. Phospholipid phosphorus determination is not needed. Phosphatidylglycerol and phosphatidylinositol, measured densitometrically, are expressed as ratios to the sphingomyelin present. These ratios are correlated with the lecithin/sphingomyelin ratio and age of gestation in normal and abnormal pregnancies.
D000653:Amniotic Fluid / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000378:metabolism; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000209:etiology; D010713:Phosphatidylcholines / Q000032:analysis; D010715:Phosphatidylglycerols / Q000032:analysis*; D010716:Phosphatidylinositols / Q000032:analysis*; D011247:Pregnancy; D011249:Pregnancy Complications, Cardiovascular / Q000378:metabolism; D011254:Pregnancy in Diabetics / Q000378:metabolism; D012127:Respiratory Distress Syndrome, Newborn / Q000209:etiology; D013109:Sphingomyelins / Q000032:analysis; D013997:Time Factors; D014132:Trachea / Q000032:analysis
D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D010716:Phosphatidylinositols; D013109:Sphingomyelins