Remyelination by cells introduced into a stable demyelinating lesion in the central nervous system.
Schwann cells from an autogeneic peripheral nerve source were injected into an established demyelinating lesion produced by the direct micro-injection of diphtheria toxin into the cat spinal cord. In control diphtheria toxin lesions, which were not injected with Schwann cells, demyelination and some oligodendrocyte remyelination was seen but Schwann cell remyelination was not observed. In diphtheria toxin lesions which were wholly confined to the posterior columns, Schwann cell myelin was not seen before 3 weeks after cell injection. The Schwann cell myelinated fibres occurred singly or in small groups within the posterior columns and were considered to have been myelinated by injected Schwann cells. By one month Schwann cell myelinated fibres had thick myelin sheaths but many demyelinated axons remained. By contrast, in more extensive diphtheria toxin lesions there was widespread Schwann cell remyelination of central axons at all stages examined after cell injection. The Schwann cell myelinated fibres were grouped in large numbers around the damaged dorsal root entry zones, the likely source of Schwann cells in these lesions. It is concluded that CNS remyelination may be improved by the injection of peripheral Schwann cells although the extent of remyelination is limited. One facet limiting remyelination may be the concentration of Schwann cells that it is possible to inject with present techniques. Functional recovery remains to be investigated.
D000818:Animals; D002415:Cats; D003711:Demyelinating Diseases / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D004167:Diphtheria Toxin; D009186:Myelin Sheath / Q000648:ultrastructure; D012583:Schwann Cells / Q000637:transplantation*; D013116:Spinal Cord / Q000473:pathology*; D013118:Spinal Cord Diseases / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D013997:Time Factors; D014182:Transplantation, Autologous
D004167:Diphtheria Toxin