title: Threshold elevation at high frequencies of the auditory nerve action potentials in acute versus chronic recordings in guinea pigs.
abstract: Thresholds of the auditory nerve compound action potential (CAP) responses to filtered clicks from 0.5 to 40 kHz, defining a CAP frequency threshold curve, have been determined in the same guinea pigs: (1) in acute conditions, the animal still anesthetized at the end of the operation for permanent implantation of a round-window electrode, and (2) several days later in the implanted awake and unrestrained animal. The results show higher CAP thresholds for high frequencies in acute conditions as compared to chronic recordings. This difference appears above about 12.5 kHz and increases progressively to around 40 dB at 40 kHz. Similar effects, although somewhat less pronounced, were observed during anesthesia alone in already implanted guinea pigs. Thes observations could explain the discrepancies which appear between electrophysiological thresholds, acutely recorded either from single fibers or whole-nerve responses, and behavioral audiograms. Moreover, in the accurate evaluation of the cochlea, slight impairments may be masked by this phenomenon as illustrated in guinea pigs treated with low doses of ototoxic antibiotic.
mesh_terms: D000161:Acoustic Stimulation / Q000379:methods*; D000758:Anesthesia; D000818:Animals; D001309:Auditory Threshold / Q000502:physiology*; D001831:Body Temperature; D005072:Evoked Potentials, Auditory; D006168:Guinea Pigs; D011930:Reaction Time; D000159:Vestibulocochlear Nerve / Q000502:physiology*