Gene composition of high-yielding influenza vaccine strains obtained by recombination.
The genetic composition of 11 high-yielding recombinants of influenza virus was determined by polyacrylamide gel electrophoresis of the 32P-labeled RNAs obtained from the recombinants and their parental viruses. The high-yield recombinants that were selected for potential use as vaccine strains contained the surface hemagglutinin and neuraminidase antigens of the low-yielding parental viruses. The increased growth capacity of the recombinants is associated with the presence of genes derived from the high-yielding laboratory strain A/Puerto Rico/8/34. Although increased growth capacity in these recombinants could not be attributed to specific genes or gene combinations, all of the high-yielding recombinants examined derived the M gene from the A/Puerto Rico/8/34 parent.
D000818:Animals; D002642:Chick Embryo; D005814:Genes, Viral; D006389:Hemagglutinins, Viral / Q000235:genetics; D009980:Influenza A virus / Q000235:genetics* / Q000254:growth & development; D007252:Influenza Vaccines / Q000145:classification*; D012367:RNA, Viral / Q000235:genetics; D011995:Recombination, Genetic
D006389:Hemagglutinins, Viral; D007252:Influenza Vaccines; D012367:RNA, Viral