[Properties of membrane lipids from maize coleoptiles].
The content and composition of lipids from the subcellular fractions of maize coleoptiles were studied. The membrane fractions were obtained by differential centrifugation and a subsequent separation in sucrose density gradient. The fractions enriched with plasmalemma, Golgi vesicles and endoplasmic reticulum were identified with some marker enzymes. The structure and lipid composition of the fractions obtained were determined. It was shown that sterins and their esters, as well as polar lipids and glycerides (mono- and triglycerides) are the main lipid components of the membrane fractions. In terms of the lipid, steroid and phospholipid composition the membranes can be arranged in the following order: plasmalemma greater than endoplasmic reticulum greater than Golgi vesicles greater than mitochondria.
D002462:Cell Membrane / Q000032:analysis; D002499:Centrifugation, Density Gradient; D007425:Intracellular Membranes / Q000032:analysis; D008563:Membrane Lipids / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D010944:Plants / Q000032:analysis*; D003313:Zea mays / Q000032:analysis
D008563:Membrane Lipids; D010743:Phospholipids
Kharakteristika lipidov membrannykh fraktsiĭ koleoptileĭ kukuruzy.