title: Factors influencing the in vitro activity of diacylglycerol acyltransferase from bovine mammary gland and liver towards butyryl-CoA and palmitoyl-CoA.
abstract: 1. Factors affecting the incorporation of butyrate relative to palmitate into the sn-3 position of triacylglycerol by the diacylglycerol acyltransferase from bovine mammary gland and liver was studied in vitro. 2. Butyrate incorporation from butyryl-CoA in the presence of palmitoyl-CoA was favoured by a high concentration of butyryl-CoA and by the presence of a long-chain acyl-CoA binding protein such as bovine serum albumin. 3. The relative activity of the enzyme from both tissues towards butyryl-CoA and palmitoyl-CoA was independent of the concentration of membrane-bound 1,2-dipalmitoylglycerol. 4. The significance of these results in relation to the unique presence of short-chain acids in ruminant milk triacylglycerols is discussed.
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism*; D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D051048:Diacylglycerol O-Acyltransferase; D004075:Diglycerides / Q000378:metabolism; D005260:Female; D008099:Liver / Q000201:enzymology*; D008321:Mammary Glands, Animal / Q000201:enzymology*; D008892:Milk / Q000378:metabolism*; D010171:Palmitoyl Coenzyme A / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014280:Triglycerides / Q000096:biosynthesis*
chemical_list: D000214:Acyl Coenzyme A; D004075:Diglycerides; D014280:Triglycerides; D010171:Palmitoyl Coenzyme A; C024343:butyryl-coenzyme A; D012710:Serum Albumin, Bovine; D000217:Acyltransferases; D051048:Diacylglycerol O-Acyltransferase