Structure-activity studies on an excitatory receptor for glutamate on leech Retzius neurones.
1 Intracellular recordings were made from Retzius cells from the segmental ganglia of Hirudo medicinalis and Haemopis sanguisuga. Glutamate had a direct excitatory effect on the leech Retzius cells. 2 L-Glutamate was 25 times more potent than D-glutamate. 3 L-Glutamate was approximately equipotent with ibotenic acid and 11.2 times more potent than L-aspartic acid. 4 Quisqualic acid and kainic acid were both approximately 100 times more potent than L-glutamate. DL-1-Amino-cis-1-3-dicarboxyclyclopentane was approximately 5 times more potent than L-glutamate, while the trans isomer was 105 times less potent. 5 alpha-NH2-pimelic acid and beta-CH3-glutamic acid reduced the response to L-glutamate. 6 It is suggested that glutamic acid may interact with the Retzius cell glutamate receptor in an extended conformation.
D000818:Animals; D005971:Glutamates / Q000494:pharmacology / Q000502:physiology*; D066298:In Vitro Techniques; D007865:Leeches / Q000502:physiology*; D008968:Molecular Conformation; D009045:Motor Endplate / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D011955:Receptors, Drug / Q000502:physiology*; D013329:Structure-Activity Relationship
D005971:Glutamates; D011955:Receptors, Drug