title: Mechanisms of E-rosette formation by mitogen-stimulated canine lymphocytes.
abstract: This study examined the mechanisms of E-rosette formation of mitogen-stimulated canine peripheral blood lymphocytes with human and guinea-pig erythrocytes (E). In vitro culture with phytohaemagglutinin-P (PHA-P) increased the number of E-rosette forming cells two- to three-fold within 1 h and ten-fold by 48 h. Mitogen-enhancement of 1 h rosettes occurred via cross-linking of E to lymphocytes by PHA-P. Forty-eight hour rosettes, mediated by natural cytophilic antibodies in serum, resulted from PHA-P-induced increases in the number of cellular Fc receptors for this immunoglobulin. The data obtained in this study indicate that E-rosette formation by resting or mitogen-stimulated canine lymphocytes is neither a reliable nor a specific T-cell marker in this species.
mesh_terms: D000116:Acetylgalactosamine / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D006801:Humans; D007074:Immunoglobulin G; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012397:Rosette Formation
chemical_list: D007074:Immunoglobulin G; D010835:Phytohemagglutinins; D000116:Acetylgalactosamine