Problems in radioimmunoassay of human lutropin with commercially available reagents.
To evaluate five commercially available reagent sets supplied for the radioimmunoassay of lutropin, we determined whether there was parallelism between the curve given by dilutions of the standards supplied by the manufacturers, by dilutions of a serum pool, and by dilutions of a standard preparation from human pituitaries, LER-907. These studies demonstrated significant analytical problems with three of the five sets. We conclude that each user should carefully evaluate all commercially available radioimmunoassays for lutropin (and, by inference, for other peptide hormones) before use.
D006801:Humans; D007202:Indicators and Reagents / Q000592:standards; D007986:Luteinizing Hormone / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods; D011933:Reagent Kits, Diagnostic / Q000592:standards
D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D007986:Luteinizing Hormone