title: Acquisition of susceptibility to coxsackievirus A2 by the rat L8 cell line during myogenic differentiation.
abstract: Coxsackievirus A2 was propagated to high titres in the established rat L8 myogenic cell line during the stage of differentiation characterized by fusion of myoblasts into multinucleated myotubes (192 h post-plating cultures). In contrast, pre-fusion mononucleated L8 cultured infected 24 h after plating were refractory to Coxsackievirus A2 infection, as was a non-fusing clonal variant of the L8 line (L8CL3-U) regardless of the age of the latter cultures. The development of virus susceptibility in the older L8 cultures correlated with a marked virus-specific c.p.e. as evidenced by vacuolated degenerating myotubes with disrupted cytoplasm, whereas no c.p.e. was seen following infection of the young L8 or variant L8CL3-U cultures. The restriction of Coxsackievirus A2 replication in young L8 and L8CL3-U cultures occurred after virus attachment.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002459:Cell Fusion; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004770:Enterovirus / Q000254:growth & development* / Q000378:metabolism; D009132:Muscles / Q000166:cytology* / Q000382:microbiology; D051381:Rats; D011991:Receptors, Virus / Q000378:metabolism
chemical_list: D011991:Receptors, Virus