Automated two-stage assay for determination of antithrombin III with a centrifugal analyzer.
The thrombin-inactivating alpha 2-globulin, antithrombin III (At-III), was measured in plasma and urine with a chromogenic tripeptide substrate, using a centrifugal analyzer. Plasma At-III was subnormal in liver disease. In acute myocardial infarction a mean At-III of approximately 120% of normal was found. At-III in persons who developed deep-vein thrombosis decreased significantly (p less than 0.05) between the day of admission and the 3rd day after admission to hospital. Urine from normals did not contain detectable At-III. With increasing urinary albumin increasing concentration of At-III was found.
D000990:Antithrombin III / Q000652:urine; D001322:Autoanalysis; D002498:Centrifugation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007185:Incubators; D009203:Myocardial Infarction / Q000175:diagnosis; D009994:Osmolar Concentration; D013696:Temperature
D000990:Antithrombin III