Studies on the formation of phosphoenolpyruvate and citric acid cycle intermediates by neonatal piglet liver mitochondria.
The capacity of mitochondria isolated from livers of 5-day-old piglets to convert pyruvate to phosphoenolpyruvate, malate and citrate has been investigated. These mitochondria have a large capacity to flux pyruvate through the pyruvate carboxylase reaction causing an accumulation of malate, citrate and phosphoenolpyruvate. As much as 70% of the pyruvate utilized during state 3 respiration can flux through the pyruvate carboxylase reaction. The data indicate that phosphoenolpyruvate from mitochondria can contribute a significant fraction of the carbon skeletons of pyruvate converted to glucose by piglet liver (estimated to be 30%).
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D002951:Citrates / Q000378:metabolism; D005943:Gluconeogenesis; D008293:Malates / Q000378:metabolism; D008930:Mitochondria, Liver / Q000378:metabolism*; D010101:Oxygen Consumption; D010728:Phosphoenolpyruvate / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D013552:Swine
D002951:Citrates; D008293:Malates; D011773:Pyruvates; D010728:Phosphoenolpyruvate