Red cell sodium and potassium in hemorrhagic shock measured by lithium substitution analysis.
A new method of measuring intracellular Na and K using Li substitution analysis was applied to a study of red cell Na changes in hemorrhagic shock. Using a rat hemorrhagic shock model, significant changes in red cell Na were found after 2 hours. Red cell Na in shocked animals was significantly higher than in control animals (p less than 0.025). A small decrease in red cell K occurred. There was no intracellular shift of Li. In addition, a fall in plasma Na and a rise in plasma K occurred with progressive shock. In a 2-hour period following retransfusion there was no significant change in cell Na or K, but the plasma Na and K returned toward preshock levels. These results confirm a prolonged and significant impairment of red cell membrane function in shock and suggest that impaired Na-K pump function may be responsible for the changes.
D000818:Animals; D004195:Disease Models, Animal; D004910:Erythrocyte Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism; D008094:Lithium / Q000097:blood; D008297:Male; D011188:Potassium / Q000097:blood*; D051381:Rats; D012771:Shock, Hemorrhagic / Q000097:blood*; D012964:Sodium / Q000097:blood*
D007473:Ion Channels; D008094:Lithium; D012964:Sodium; D011188:Potassium