Influence of brinase on fibrinogen: fibrin transition in in vitro and in vivo studies. I. Influence of in vitro addition of brinase to plasma on the ethanol gelation test.
The incidence of positive ethanol gelation test (EGT) after addition of brinase (a proteolytic enzyme preparation from Aspergillus oryzae) to anticoagulated and non-anticoagulated human plasma was studied. In vitro addition of brinase to plasma causes positive EGT, and the incidence is dose-dependent. In plasma from warfarin-treated patients and/or after addition of heparin to plasma, prior to the addition of brinase, a significantly reduced incidence of positive EGT is observed. The incidence is lowest after heparin. Gels formed in the presence of heparin are easier susceptible to enzymatic degradation than those formed in the absence of heparin.
D001780:Blood Coagulation Tests; D001954:Brinolase / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000431:Ethanol; D005337:Fibrin / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism*; D005782:Gels; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D010447:Peptide Hydrolases / Q000494:pharmacology*; D013997:Time Factors; D014652:Vascular Diseases / Q000188:drug therapy; D014859:Warfarin / Q000627:therapeutic use
D005782:Gels; D000431:Ethanol; D014859:Warfarin; D005337:Fibrin; D005340:Fibrinogen; D006493:Heparin; D010447:Peptide Hydrolases; D001954:Brinolase