title: Nickel, a component of factor F430 from Methanobacterium thermoautotrophicum.
abstract: Methanobacterium thermoautotrophicum, growing on medium supplemented with 2 mumol 63NiCl2/l, was found to take up 1.2 mumol 63Ni per g cells (dry weight). More than 70% of the radioisotope was incorporated into a compound, which dissociated from the protein fraction after heat treatment, was soluble in 70% acetone, and could be purified by chromatography on QAE-Sephadex A-25, Sephadex G-25, and DEAE cellulose. The purified 63Ni labelled compound had an absorption spectrum and properties identical to those of factor F430 and is therefore considered to be identical with factor F430.
mesh_terms: D002458:Cell Fractionation; D019605:Euryarchaeota / Q000032:analysis* / Q000378:metabolism; D005415:Flavins / Q000032:analysis; D008665:Metalloporphyrins; D008970:Molecular Weight; D009532:Nickel / Q000032:analysis* / Q000378:metabolism; D013347:Subcellular Fractions / Q000032:analysis
chemical_list: D005415:Flavins; D008665:Metalloporphyrins; C023540:factor F430; D009532:Nickel