DNA polymerases in parasitic protozoans differ from host enzymes.
Analysis of extracts of the bloodstream forms of Trypanosoma brucei showed that both DNA polymerase-alpha and DNA polymerase-beta activities were present. The detection of DNA polymerase-beta in T. brucei demonstrates the presence of this enzyme in unicellular organisms. DNA polymerase-beta is present also in Leishmania mexicana. The DNA polymerases in T. brucei are immunologically distinct from the host enzymes. The structural differences between the parasite and the host enzymes could be exploited for the development of agents to combat parasitic diseases.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002645:Chickens; D004256:DNA Polymerase I / Q000032:analysis; D004257:DNA Polymerase II / Q000032:analysis; D004258:DNA Polymerase III / Q000032:analysis; D004259:DNA-Directed DNA Polymerase / Q000032:analysis*; D005399:Fishes; D007106:Immune Sera; D007891:Leishmania / Q000201:enzymology*; D008970:Molecular Weight; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D014346:Trypanosoma brucei brucei / Q000201:enzymology*
D007106:Immune Sera; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase