title: Adherence of L1210 murine leukemia cells to sephacryl-aminopropylcobalamin beads treated with transcobalamin-II.
abstract: Sephacryl beads containing an immobilized aminopropylcobalamin-transcobalamin-II complex serve as foci for the adherence of L1210 murine leukemia cells. Bead-cell interaction does not occur when (A) nonderivatized beads are used; (B) transcobalamin-II is omitted or presaturated with cyanocobalamin in the preparation of the bead complex; (C) intrinsic factor replaces transcobalamin-II; and (D) the complex is removed from beads by photolysis. These observations suggest that adherence results from the ability of transcobalamin-II to form a bridge between immobilized cobalamin on the bead and receptors in the plasma membrane of the cell.
mesh_terms: D000180:Acrylic Resins; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002352:Carrier Proteins; D002448:Cell Adhesion; D007939:Leukemia L1210 / Q000378:metabolism* / Q000648:ultrastructure*; D051379:Mice; D011817:Rabbits; D014155:Transcobalamins / Q000378:metabolism*; D014805:Vitamin B 12 / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D000180:Acrylic Resins; D001798:Blood Proteins; D002352:Carrier Proteins; D014155:Transcobalamins; C022971:aminopropylcobalamin; D014805:Vitamin B 12