Analysis of drugs by pyrolysis. II--An improved method for the determination of carpronium chloride in plasma by selected ion monitoring.
A sensitive and specific method has been devised for the determination of carpronium chloride, a parasympathomimetic agent, in plasma. The method is based on selected ion monitoring combined with the pyrolysis reaction of (3-isopropoxycarbopropyl)trimethylammonium chloride derived by transesterification of the drug. Carpronium chloride-[2H9] is used as an internal standard. The drug and the internal standard are separated from deproteinized plasma by ion pair extraction, and converted into isopropyl ester derivatives. The derivatives are pyrolysed to produce the respective isopropyl N,N-dimethyl-gamma-aminobutyrates available for selected ion monitoring. Measurement is carried out in the electron impact mode by monitoring the fragment ion [M-OC3H7]+ (m/z 114, 120). The method is applicable to 5 ng ml-1 plasma with about 10% coefficient of variation, and has been utilized successfully for the pharmacokinetics of carpronium chloride in man.
D000818:Animals; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D008954:Models, Biological; D010277:Parasympathomimetics / Q000097:blood*; D000644:Quaternary Ammonium Compounds / Q000097:blood; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives* / Q000097:blood
D010277:Parasympathomimetics; D000644:Quaternary Ammonium Compounds; D005680:gamma-Aminobutyric Acid; C001817:carpronium