Hybridomas secreting monoclonal antibody with specificity for Toxoplasma gondii.
This study reports the successful establishment of eight functional hybridomas between mouse myeloma cells and spleen cells from mice hyperimmune to T. gondii as monitored by indirect immunofluorescence and radioimmunoassay techniques. Two of these eight cloned hybridomas showed positive reactivity in the Sabin Feldman dye test. Three hybridoma clones selected, following fusion of myeloma cells with freshly harvested spleen cells from hyperimmune mice, secreted into the culture medium antitoxoplasma antibody fo the IgC class. When spleen cells from hyperimmune mice were precultured in vitro for 5 days in presence of Con A and LPS and then fused with myeloma cells, five of the stable hybridomas secreted antitoxoplasma antibody of the IgM class, while one secreted antibody of the IgG class. These monoclonal antitoxoplasma antibodies should permit a rational approach to the purification of corresponding antigenic molecules from toxoplasma.
D000818:Animals; D000917:Antibody Formation; D002459:Cell Fusion; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D006822:Hybrid Cells / Q000276:immunology*; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D008214:Lymphocytes; D051379:Mice; D009101:Multiple Myeloma; D014122:Toxoplasma / Q000276:immunology*
D007074:Immunoglobulin G; D007075:Immunoglobulin M