title: Study of the interaction between L-lactate dehydrogenase isoenzymes and immobilized 8-substituted adenosine 5'-monophosphate by means of affinity electrophoresis.
abstract: A water-soluble 8-substituted adenosine 5'-monophosphate-polyacrylamide (8-sub-5'-AMP-PA) was prepared as an affinity ligand for the affinity electrophoresis of L-lactate dehydrogenase (LDH). According to the principles of affinity electrophoresis, the dissociation constants of the interaction between immobilized 8-sub-5'-AMP and rabbit LDH isoenzymes [LDH-1(H4), LDH-2(H3M), LDH-3(H2M2), LDH-4(HM3) and LDH-5(M4)], beef LDH-1(H4) and LDH-5(M4) and pig LDH-1(H4) were calculated. Both rabbit and beef LDH-5 (muscle-type isoenzymes) had approximately a 35-fold stronger affinity to immobilized 8-sub-5'-AMP than has their LDH-1 (heart-type isoenzymes). The effects of free nucleotides on the interaction between immobilized 8-sub-5'-AMP and LDH isoenzymes were also investigated by affinity electrophoresis. NAD+ has a stronger affinity to LDH-5(M4) than had 5'-AMP and 5'-IMP, while NMN, adenosine and fructose 6-phosphate had no affinity.
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007527:Isoenzymes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D011817:Rabbits; D013552:Swine
chemical_list: D007527:Isoenzymes; D000249:Adenosine Monophosphate; D007770:L-Lactate Dehydrogenase