title: Induction of sister chromatid exchanges by hydroxylamine, hydrazine and isoniazid and their inhibition by cysteine.
abstract: Experiments were performed in order to gain information about the primary process leading to the production of sister chromatid exchanges (SCEs). Radical-forming substances (hydroxylamine, hydrazine and the antituberculous drug isoniazid) were examined for their effectiveness in inducing SCEs. All three substances proved successful in the induction of SCEs in the V-79 cell line of the Chinese hamster. By simultaneous application of a sulfhydryl compound (cysteine), a reduction of the hydrazine- and isoniazid-induced SCEs was achieved. Isoniazid was additionally examined in the in vivo SCE-test. At concentrations of 2-100 mg/kg body weight, it does not increase the rate of SCEs in the bone marrow of the Chinese hamster.
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D003434:Crossing Over, Genetic / Q000187:drug effects*; D003545:Cysteine / Q000494:pharmacology*; D003864:Depression, Chemical; D006834:Hydrazines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006898:Hydroxylamines / Q000494:pharmacology*; D066298:In Vitro Techniques; D007538:Isoniazid / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D013268:Stimulation, Chemical
chemical_list: D006834:Hydrazines; D006898:Hydroxylamines; D003545:Cysteine; D007538:Isoniazid