Influence of turbidity on photometric assays: a blank sample must always be used.
The authors studied the influence of sample turbidity on photometric assays at different wavelengths. Turbidity was created by using a mixture of hydrazine sulfate and hexamethylene tetramine, and a dye solution (p-nitrophenol, cobalt nitrate). This work demonstrates the necessity of using a bland sample, for example in the glucose assay (glucose oxidase/peroxidase). The influence of turbidity on an assay depends upon the instrument. Knowledge of this influence is essential for the evaluation of the viability of an assay in hyperlipemic serum.
D001786:Blood Glucose / Q000032:analysis; D005189:False Positive Reactions; D005949:Glucose Oxidase; D006593:Hexokinase; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D010544:Peroxidases; D011786:Quality Control; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods*
D001786:Blood Glucose; D005949:Glucose Oxidase; D010544:Peroxidases; D006593:Hexokinase