Comparative experiments with hemolytic streptococcus and its anticancer preparations (OK-431 and OK-432) for their cytolytic activity.
The comparative in vitro cytolytic effects of living hemolytic streptococcus, a low virulent su-strain, and its streptococcal preparations (OK-431 and OK-432) on Ehrlich ascites carcinoma cells were investigated. It was shown that living cocci and OK-431 released 51Cr from 51Cr-labeled tumor cells by contact in vitro, but OK-432 did not release 51Cr at all. Moreover, the effects of living cocci, OK-431 and OK-432 were also examined by assay of the release of RNA and DNA from the tumor cells. It was confirmed that living cocci released RNA and DNA, but OK-431 released slightly RNA, not DNA from the tumor cells, whereas OK-432 did not cause these release at all. Consequently, it appeared that living cocci of Su-strain have more cytolytic activity than OK-431, and that OK-432 have no cytolytic activity in tumor cells.
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D001688:Biological Products / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D004273:DNA, Neoplasm / Q000378:metabolism; D051379:Mice; D010844:Picibanil / Q000494:pharmacology*; D012334:RNA, Neoplasm / Q000378:metabolism; D013291:Streptococcus
D000972:Antineoplastic Agents, Phytogenic; D001688:Biological Products; D002860:Chromium Radioisotopes; D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D010844:Picibanil