[Cultivation of amniotic fluid cells for the purpose of diagnosing certain metabolic diseases].
A method for preparing amniotic fluid cell culture has been developed. The optimal conditions for the culture involved the incubation period averaging 17.9 days. Normal activity values were obtained for 8 glycosidases whose deficiency is responsible for the development of hereditary accumulation diseases. The data obtained allow a prenatal diagnosis of glycolipidoses (Tay-Sachs, Fabrys, Gaucher's diseases, etc.). The evidence on prenatal diagnosis of Tay-Sach's disease is presented.
D000653:Amniotic Fluid / Q000166:cytology*; D002478:Cells, Cultured; D005260:Female; D006026:Glycoside Hydrolases / Q000172:deficiency*; D006801:Humans; D008661:Metabolism, Inborn Errors / Q000175:diagnosis*; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D013661:Tay-Sachs Disease / Q000175:diagnosis
D006026:Glycoside Hydrolases
Kul'tivirovanie kletok amnioticheskoĭ zhidkosti v tseliakh prenatal'noĭ diagnostiki nekotorykh bolezneĭ obmena.