Partitioning of staphylococcal delta haemolysin.
The behaviour of the biologically active components present in crude delta haemolysin was followed during various fractionation procedures utilised in the purification of delta haemolysin. Most chromatographic techniques yielded multiple peaks of delta haemolysin. None of the procedures completely separated delta haemolysin from the other components of crude culture filtrates. Efforts to purify delta haemolysin should be renewed.
D002266:Carboxymethylcellulose Sodium; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006460:Hemolysin Proteins / Q000302:isolation & purification* / Q000502:physiology; D006821:Hyaluronoglucosaminidase / Q000032:analysis; D006867:Hydrolases / Q000032:analysis; D006882:Hydroxyapatites; D008049:Lipase / Q000032:analysis; D009113:Muramidase / Q000032:analysis; D013211:Staphylococcus aureus
D006460:Hemolysin Proteins; D006882:Hydroxyapatites; D006867:Hydrolases; D008049:Lipase; D009113:Muramidase; D006821:Hyaluronoglucosaminidase; D002266:Carboxymethylcellulose Sodium