Neutralization of cytotoxicity of spermine on the proliferation of rat liver cells in tissue culture.
Cytotoxicity of spermine in tissue culture was found previously. To neutralize this toxicity, the addition of various high molecular weight substances and others was attempted in this paper, e.g. lysozyme, N-acetyl-D-glucosamine, chondroitin sulfate, poly-L-glutamic acid, bovine serum fractions V and VI, fetal calf serum, methyl cellulose, carboxymethyl cellulose, polyvinylpyrrolidone and others. Into the culture of rat liver cells, strain RLC-10(2), simultaneous addition of other substances with spermine did not neutralize the toxicity. However, by the pretreatment of spermine with fetal calf serum or bovine serum albumin (fraction V) at 37 degrees C for 24 hr, the toxicity of spermine was markedly reduced. This was probably due to the denaturation of spermine caused by the pretreatment.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D046508:Culture Techniques; D008099:Liver / Q000166:cytology*; D008970:Molecular Weight; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D013096:Spermine / Q000633:toxicity*
D012710:Serum Albumin, Bovine; D013096:Spermine