Inhibition of exogenous mRNA translation in a cell-free system by chromatin peptides from calf thymus.
Low molecular weight chromatin peptides isolated from calf thymus by affinity chromatography on DNA-cellulose inhibit significantly translation of exogenous isolated mRNA in a reticulocyte cell-free system. Translation with endogenous mRNA present in the system is not inhibited by low peptide concentrations. The data obtained combined with the previous findings suggest that chromatin peptides control gene expression at two levels: transcription and translation.
D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D002843:Chromatin / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D007700:Kinetics; D009698:Nucleoproteins / Q000494:pharmacology*; D010455:Peptides / Q000302:isolation & purification / Q000494:pharmacology*; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism*; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D013950:Thymus Gland / Q000502:physiology*
D002843:Chromatin; D009698:Nucleoproteins; D010455:Peptides; D012333:RNA, Messenger