Determination of avermectins in plasma at nanogram levels using high-performance liquid chromatography with fluorescence detection.
An analytical method is described for the determination of the avermectins in plasma based upon high-performance liquid chromatography of fluorescent derivatives of these compounds. The analyte is isolated by adsorption chromatography on Florisil, dehydrated in an acetic anhydride-pyridine mixture, and the fluorophore is further separated by chromatography on silica gel in advance of introduction into a reversed-phase system. This method, which can be applied to samples containing as little as 0.2 ng drug per ml, has an accuracy of 5% mean relative error and a precision of 8% relative standard deviation. A study and discussion of several factors which affect the analytical reaction are included.
D000818:Animals; D000871:Anthelmintics; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004187:Disaccharides / Q000097:blood; D007559:Ivermectin / Q000031:analogs & derivatives*; D007783:Lactones / Q000097:blood*; D013050:Spectrometry, Fluorescence
D000871:Anthelmintics; D004187:Disaccharides; D007783:Lactones; D007559:Ivermectin; C019264:avermectin