title: Determination of uric acid in serum using isotachophoresis.
abstract: An operational system is described for the isotachophoretic determination of uric acid in serum, making use of column coupling. The method has been compared with a standard enzymatic procedure. With the present technique small amounts of serum (ca. 3 microliter) can be applied without any pretreatment. Urate recovery was 99.0-100.5%. Under the non-physiological measuring conditions used, 12-28% of control serum uric acid was bound to macromolecules of molecular weight exceeding 25,000. The day-to-day variations of the isotachophoretic procedure were smaller than those of the enzymatic method, whereas standard deviations were comparable. The isotachophoretic procedure is less influenced by certain metabolites.
mesh_terms: D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000097:blood; D004586:Electrophoresis / Q000379:methods; D005260:Female; D006073:Gout / Q000097:blood; D006801:Humans; D008297:Male; D008832:Microchemistry; D008875:Middle Aged; D012016:Reference Values; D014503:Urate Oxidase; D014527:Uric Acid / Q000097:blood*
chemical_list: D014527:Uric Acid; D014503:Urate Oxidase