title: Estimation of progesterone in buffalo milk and its application to pregnancy diagnosis.
abstract: Milk samples were collected on Days 20, 24, 28 and 40 after insemination. The percentage of fat and thr progesterone concentration in the milk were correlated (P < 0.01). Progesterone concentration in the milk of pregnant buffaloes (24.83 +/- 3.85 ng/ml) was significantly higher than that in non-pregnant animals (2.89 +/- ng/ml) on Day 20 and the difference between the two increased with time after insemination. The detection of non-pregnant animals was 100% successful at all times but the diagnosis was correct for 66, 68, 81 and 83% of animals tested on Days 20, 24, 28 and 40 respectively and predicted as pregnant.
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000378:metabolism*; D005260:Female; D008892:Milk / Q000378:metabolism*; D011247:Pregnancy; D011258:Pregnancy Tests / Q000662:veterinary; D011270:Pregnancy, Animal; D011374:Progesterone / Q000378:metabolism*
chemical_list: D011374:Progesterone