title: Use of chromatography tubes in triiodothyronine and thyroxine RIA.
abstract: Simple, convenient radioimmunoassays for total triiodothyronine and total thyroxine in human serum are discribed. Ascending dry column chromatography is used to separate the free and antibody-bound hormone. The method allows for separation by absorption, without centrifugation. The use of test tubes containing pre-measured quantities of lyophilized radioactive reagent obviates unnecessary handling of radioactive solutions.
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D013974:Thyroxine / Q000097:blood*; D014284:Triiodothyronine / Q000097:blood*
chemical_list: D014284:Triiodothyronine; D013974:Thyroxine