title: Hybrid pathway for chlorobenzoate metabolism in Pseudomonas sp. B13 derivatives.
abstract: Derivatives of Pseudomonas sp. B13 which had acquired the capability to utilize 4-chloro- and 3,5-dichlorobenzoate as a consequence of the introduction of genes of the TOL plasmid of Pseudomonas putida mt-2 were studied. The utilization of these substrates, a property not shared by the parent strains, was shown to depend upon the combined activities of enzymes from the donor and from the recipient. During growth on 3-chloro-, 4-chloro-, and 3,5-dichlorobenzoate, predominantly the toluate 1,2-deoxygenase and both dihydrodihydroxybenzoate dehydrogenases of the parent strains were induced. On the other hand, no catechol 2,3-dioxygenase from P. putida mt-2 was detectable, so that degradation of chlorocatechols by the nonproductive meta-cleavage pathway was avoided. Instead of that, chlorocatechols were subject to ortho cleavage and totally degraded by the preexisting enzymes of Pseudomonas sp. B13.
mesh_terms: D050548:Catechol 1,2-Dioxygenase; D002396:Catechols / Q000378:metabolism; D002723:Chlorobenzoates / Q000378:metabolism*; D003227:Conjugation, Genetic; D049308:Dioxygenases; D004790:Enzyme Induction; D010088:Oxidoreductases / Q000096:biosynthesis; D010105:Oxygenases / Q000096:biosynthesis / Q000378:metabolism; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D014050:Toluene / Q000096:biosynthesis
chemical_list: D002396:Catechols; D002723:Chlorobenzoates; D014050:Toluene; D010088:Oxidoreductases; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; C023172:toluene dioxygenase; C025312:3,5-cyclohexadiene-1,2-diol-1-carboxylate dehydrogenase