Estimation of PHA induced transformation in peripheral blood lymphocytes through measurement of their increased glycolysis. An enzymatic micromethod.
Peripheral blood lymphocytes undergoing blast transformation after contact with PHA, show a rapid increase in glycolysis. The phenomenon can be quantitated by measuring the amount of lactate released in the culture medium. This measurement has been adapted to a micromethod which allows the study of PHA induced transformation in a large number of samples in a short time. The present method which avoids the use of radioisotopes correlates in 90% of the cases with the 3H-thymidine incorporation test.
D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006019:Glycolysis; D006801:Humans; D007773:Lactates / Q000378:metabolism; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D007773:Lactates; D010835:Phytohemagglutinins; D013936:Thymidine