Electrophoretic injection of a fluorescent dye into giant mitochondria and mitoplasts.
Studies of the electrical properties of giant mitochondria and mitoplasts with microelectrodes have indicated that there are no significant metabolically dependent membrane potentials. The internal location of the microelectrode has been confirmed by electrophoretically microinjecting the water-soluble dye Lucifer yellow CH into giant mitochondria or mitoplasts.
D000818:Animals; D004586:Electrophoresis; D005456:Fluorescent Dyes / Q000008:administration & dosage*; D008564:Membrane Potentials; D051379:Mice; D008839:Microelectrodes; D008930:Mitochondria, Liver / Q000502:physiology* / Q000648:ultrastructure
D005456:Fluorescent Dyes