Effect of diet and drugs on the qualitative and quantitative distribution of cytochromes P-450 in rat liver.
Male Sprague-Dawley rats were fed either Purina chow or a nutritionally adequate liquid control diet containing either 4 or 38% of the total caloric intake as fat. The hepatic cytochromes -450 were induced in these animals either by the administration of phenobarbital, beta-naphthoflavone, or isocaloric replacement of the carbohydrate of the liquid diets by ethanol at a level of 36% of total calories for 4 or 6 weeks. Three distinct groups of cytochromes P-450 could be efficiently separated by ion-exchange chromatography of microsomal preparations from these rats. The concentration of each group of cytochromes P-450 was markedly affected by variations in lipid and carbohydrate content of the diet as well as by administration of drugs.
D000818:Animals; D001835:Body Weight; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004032:Diet; D000431:Ethanol / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D019324:beta-Naphthoflavone
D005419:Flavonoids; D000431:Ethanol; D019324:beta-Naphthoflavone; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital