Regulation of growth of newborn rat heart cell cultures: influence of cell density, pH, and a platelet-derived factor.
The search for elements regulating cardiac cell growth was undertaken using heart cell cultures as a model system. This model, derived from newborn rats, allowed the approach of the regulation of growth both at the cellular level and at a stage of incomplete differentiation of the muscle cells. The major control elements that have been identified are: 1) availability of essential nutrients; 2) proper regulation of the pH in the surrounding medium; 3) presence of serum survival factors and serum growth factors; and 4) homotypic and heterotypic cellular interactions. Specific examples pertaining to these different control elements are presented.
D000818:Animals; D000831:Animals, Newborn; D001769:Blood; D001792:Blood Platelets / Q000378:metabolism*; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D006133:Growth Substances; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D013045:Species Specificity; D013997:Time Factors
D003470:Culture Media; D006133:Growth Substances