title: Histone acetylation in differentiating chick myoblast cultures.
abstract: Histone acetyltransferase activity in cell extracts from chick myoblast cultures increases up to 24h of cell culture and then decreases steadily to about 30-40% of the 24h value after 6 to 7 days. The decrease in specific activity is independent of the initial cell density. Histone acetylation in intact cells has also been studied by tritiated-acetate labelling. Most of the radioactivity is found in histones H2b, H3 and H4 when separated on acid-urea gels, and is not prevented by puromycin.
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D006657:Histones / Q000378:metabolism*; D009132:Muscles / Q000166:cytology* / Q000201:enzymology; D011691:Puromycin / Q000494:pharmacology
chemical_list: D006657:Histones; D011691:Puromycin; D000123:Acetyltransferases