Proximity of mRNA5'-region and 18S rRNA in eukaryotic initiation complexes.
Messenger RNA was covalently linked to 18S ribosomal RNA in eukaryotic 40S and 80S initiation complexes by photoreaction with an RNA cross-linking agent, 4'-substituted psoralen. The sites of interaction of the mRNA capped 5'-region included some 3'-ends of 18S rRNA.
D000818:Animals; D001483:Base Sequence; D003432:Cross-Linking Reagents; D005057:Eukaryotic Cells / Q000502:physiology; D011564:Furocoumarins; D006860:Hydrogen Bonding; D008970:Molecular Weight; D010442:Peptide Chain Initiation, Translational; D012333:RNA, Messenger / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D011817:Rabbits; D014908:Triticum
D003432:Cross-Linking Reagents; D011564:Furocoumarins; D012333:RNA, Messenger; D012335:RNA, Ribosomal