Multiple esterase forms in isolated hepatocytes and Kupffer cells of partially hepatectomized rats.
The esterase patterns of isolated parenchymal liver cells of rats consisted of 6 bands of enzymatic activity, whereas the patterns of iron-loaded Kupffer cells showed 5 bands. Both patterns become simpler in the early prereplicative period of liver regeneration. Dukring simultaneous replication of DNA, i.e. 24 h after partial liver removal, an additional band of esterase activity appears in patterns of hepatocytes and Kupffer cells. At the moment of maximum hepatocyte mitotic rate, i.e. 36 h after partial hepatectomy, both esterase patterns lose the single band of activity again. 2 or 3 days after surgery the initial esterase patterns in hepatocytes return whereas the patterns of Kupffer cells remain incomplete.
D000818:Animals; D004592:Electrophoresis, Starch Gel; D004950:Esterases / Q000378:metabolism*; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D007728:Kupffer Cells / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008115:Liver Regeneration; D008297:Male; D051381:Rats
D007527:Isoenzymes; D004950:Esterases