LDH isozyme analyses of ethylnitrosourea-induced central nervous system tumors in rats.
To provide the significance of LDH isozymes in rat CNS tumors, the changes in lactic dehydrogenase isozyme and calculated ratios of H- to M- subunit were studied by means of polyacrylamide gel enzymoelectrophoresis in tumor extracts from CNS tumors (7 astrocytomas, 4 oligodendrogliomas, 7 mixed gliomas, 6 anaplastic gliomas, 3 glioependymomas, 1 astroblastoma, 11 neurinomas, 8 anaplastic neurinomas and 1 meningioma in Wistar rats which were induced by ethylnitrosourea). The isozyme patterns were compared to those obtained from normal rat CNS tissues. Among the glioma group, oligodendroglioma showed the highest H/M ratio followed by mixed glioma, glioependymoma, astrocytoma, astroblastoma and anaplastic glioma in order of decreasing of H/M ratios. On the other hand, the H/M ratio of neurinoma was significantly higher than that of anaplastic neurinoma. These observation suggested that determination of LDH isozyme patterns could supplement the histological evaluation of brain tumors.
D000818:Animals; D001254:Astrocytoma / Q000139:chemically induced / Q000201:enzymology; D001932:Brain Neoplasms / Q000139:chemically induced / Q000201:enzymology*; D005038:Ethylnitrosourea / Q000633:toxicity*; D005260:Female; D005910:Glioma / Q000139:chemically induced / Q000201:enzymology; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology; D009442:Neurilemmoma / Q000139:chemically induced / Q000201:enzymology; D009607:Nitrosourea Compounds / Q000633:toxicity*; D009837:Oligodendroglioma / Q000139:chemically induced / Q000201:enzymology; D051381:Rats; D013116:Spinal Cord / Q000201:enzymology
D007527:Isoenzymes; D009607:Nitrosourea Compounds; D007770:L-Lactate Dehydrogenase; D005038:Ethylnitrosourea