The isolated-perfused rabbit oviduct: a simple experimental preparation.
A new, simple method to study several physiological parameters in the isolated rabbit oviduct is described in detail. The whole oviduct with its supporting membranes, the ovary and part of the uterine horn, and part of the systemic blood supply were excised and perfused in vitro through cannulated blood vessels with a blood-physiological solution mixture. Autoregulation of the vasculature was demonstrated, suggesting that the preparation was viable and well oxygenated. The frequency of contractions of the ampullary and isthmic parts was decreased by prostaglandin E2 added to the perfusing solution.
D000818:Animals; D001833:Body Temperature Regulation; D005187:Fallopian Tubes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D010066:Ovum Transport / Q000187:drug effects; D010477:Perfusion; D011458:Prostaglandins E / Q000494:pharmacology; D011817:Rabbits
D011458:Prostaglandins E