Purification of free eukaryotic initiation factors eIF-4A and eIF-4D on Cibacron Blue F3G-A.
The eukaryotic initiation factors eIF-4A and eIF-4D are almost exclusively found in the supernatant of a reticulocyte lysate. The basic steps of general purification schemes failed because these factors do not bind to Sepharoseheparin or, in the of eIF-4A, to phosphocellulose and new procedure had to be devised. Cibacron Blue F3G-A proved to be a successful alternative in the purification of these factors from the excessive load of proteins found in the supernatant.
D000818:Animals; D000873:Anthracenes; D002474:Cell-Free System; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D039601:Eukaryotic Initiation Factor-4A; D005590:Fractional Precipitation; D010448:Peptide Initiation Factors / Q000302:isolation & purification*; D016601:RNA-Binding Proteins; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis*; D014227:Triazines; D000097574:Eukaryotic Translation Initiation Factor 5A
D000873:Anthracenes; D039601:Eukaryotic Initiation Factor-4A; D010448:Peptide Initiation Factors; D016601:RNA-Binding Proteins; D014227:Triazines; D000097574:Eukaryotic Translation Initiation Factor 5A; C012842:Cibacron Blue F 3GA