Spectrofluorometric determination and thin layer chromatographic identification of serotonin in foods.
A method is described for determining serotonin in foods, based on alkaline butanol-sand column elution followed by spectrofluorometric and spectrofluorometric determination with thin layer chromatographic confirmation. The method has been applied to fresh bananas, banana-based baby food, and fresh and canned tomatoes. The average recovery was 91%. The amounts of serotonin found were 10--30 ppm in bananas, 0.1--1.9 ppm in banana-based baby foods, 4.4--5.6 ppm in fresh tomatoes, and 2.8--5.6 ppm in canned tomatoes.
D002855:Chromatography, Thin Layer; D005504:Food Analysis; D005638:Fruit / Q000032:analysis; D007225:Infant Food / Q000032:analysis; D012701:Serotonin / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D014675:Vegetables / Q000032:analysis
D012701:Serotonin