Immunogenicity of ovalbumin-Ficoll conjugates with particular reference to IgE antibody production.
Conjugates of ovalbumin with Ficoll (OA-Ficoll) were injected into BDF1 mice to establish their immunogenicity and specifically to determine their effect on the IgE anti-OA response. Mice injected with OA-Ficoll responded by producing IgG anti-OA, but not IgE anti-OA. Furthermore, soluble OA-Ficoll injected subsequent to native OA in the form optimal to elicit IgE antibody in fact suppressed the production of IgE anti-OA. Spleen cells from OA-Ficoll-injected mice when injected into normal mice suppressed the subsequent IgE response to OA. OA-Ficoll reacted only weakly with antibody to native OA and had no effect on the IgE antibody response to the unrelated antigen alpha-amylase.
D000818:Animals; D000917:Antibody Formation; D004136:Dinitrobenzenes / Q000276:immunology; D005362:Ficoll / Q000276:immunology*; D005947:Glucose / Q000276:immunology; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007136:Immunoglobulins / Q000145:classification / Q000276:immunology; D051379:Mice; D010047:Ovalbumin / Q000276:immunology*; D011108:Polymers; D011134:Polysaccharides / Q000276:immunology*
D004136:Dinitrobenzenes; D007136:Immunoglobulins; D011108:Polymers; D011134:Polysaccharides; D005362:Ficoll; D007073:Immunoglobulin E; D010047:Ovalbumin; D005947:Glucose