Effects of anesthetic alcohols on membrane transport processes in human erythrocytes.
1. Anesthetic alcohols (pentanol, hexanol and heptanol) were found to increase the fluidity of red cell membrane lipids as monitored by the fluorescence depolarization of diphenylhexatriene. The relative potency of the alcohols was found to be parallel to their relative membrane/water partition coefficients. 2. Hexanol had biphasic effect on erythritol uptake by simple diffusion by red cells. At concentrations less than 9 mM, there was an approximately linear increase in erythritol permeability with increasing alcohol concentration. 3. The facilitated transport of uridine was markedly inhibited by hexanol. Hexanol at 6 mM produced a 65% inhibition of uridine (4 mM) uptake. Hexanol decreased both the apparent Km and V values for the equilibrium exchange of uridine. 4. The facilitated transport of galactose was only slightly inhibited by hexanol. 5. Hexanol was without effect on the passive and active fluxes of Na+ and K+ in red cells with altered cation contents. Cells that were slightly depleted of K+ and cells that were highly K+ -depleted were both insensitive to hexanol.
D000438:Alcohols / Q000494:pharmacology*; D000777:Anesthetics / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D004058:Diffusion; D004896:Erythritol / Q000097:blood; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005690:Galactose; D005693:Galactosemias; D006801:Humans; D007700:Kinetics; D008560:Membrane Fluidity / Q000187:drug effects*; D011188:Potassium / Q000097:blood; D012964:Sodium / Q000097:blood; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D014529:Uridine / Q000097:blood
D000438:Alcohols; D000777:Anesthetics; D012964:Sodium; D004896:Erythritol; D011188:Potassium; D014529:Uridine; D005690:Galactose