title: Replicative and unscheduled DNA synthesis in adriamycin-treated myocardial cells.
abstract: The effect of the potent antitumor antibiotic adriamycin (ADM) on chromosome integrity, DNA replication, and unscheduled DNA synthesis was investigated in cultured rat cardiac cells. Chromosome distribution, autoradiography, and [3H]thymidine (dThd) incorporation studies were carried out on separate cultures. A 3-hr pulse of ADM at a concentration of 1 microgram/ml was sufficient to cause chromosomal aberrations that were evident for up to 3 days post-ADM treatment. At the same ADM dosage, DNA replication was depressed for up to 6 days by as much as 90%, yet the ability of the cardiac cells to repair UV-damaged DNA was not impaired. However, cells exposed to higher concentrations of ADM failed to undergo significant UV-induced repair.
mesh_terms: D000818:Animals; D001345:Autoradiography; D002875:Chromosomes; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects* / Q000528:radiation effects; D004317:Doxorubicin / Q000494:pharmacology*; D006918:Hydroxyurea / Q000494:pharmacology; D008938:Mitosis; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D013936:Thymidine / Q000378:metabolism
chemical_list: D004317:Doxorubicin; D004247:DNA; D013936:Thymidine; D006918:Hydroxyurea