Separation of cyanogen bromide peptides of collagen by means of high-performance liquid chromatography.
Cyanogen bromide peptides of bovine collagen Types I, II nad III were analyzed using high-performance liquid chromatography (HPLC). Elution patterns of each collagen type were unique and reproducible. Elution patterns of the CNBr peptides of the alpha 1 and alpha 2 chains of Type I collagen were also unique and together accounted for the major components of Type I collagen. Analysis of the eluted peptides from HPLC of each collagen type by sodium dodecyl sulphate-polyacrylamide gel electrophoresis showed specific patterns for each collagen. Thus, unique and reproducible HPLC chromatograms were obtained, providing a new analytical method that is simple, sensitive and rapid.
D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003094:Collagen / Q000032:analysis*; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D010455:Peptides / Q000032:analysis*; D012867:Skin / Q000032:analysis; D013977:Tibia / Q000032:analysis
D010455:Peptides; D003094:Collagen; D003488:Cyanogen Bromide