title: Nitrogen balance, abomasal crude protein and amino acids in wethers fed formaldehyde-treated coastal bermudagrass and infused with methionine, glucose or monensin.
abstract: A 5 X 5 Latin square design was used to study the effects on nitrogen balance, wool growth and plasma amino acids of infusing methionine and (or) glucose into the abomasum of growing wethers fed formaldehyde-treated Coastal bermudagrass (F-CBG) or untreated Coastal bermudagrass hay. Daily treatments were: (1) CBG; (2) F-CBG + 3 g methionine (met); (4) F-CBG + 96 g glucose and (5) F-CBG + 3 met + 96 g glucose. Glucose infusion decreased (P < .005) moles/100 g of total essential amino acids in plasma; moles/100 g of lysine, valine, leucine and isoleucine were also reduced (P < .005). Moles/100 g of methionine in plasma were increased significantly by methionine infusion, but nitrogen retention was not affected (P > .10). The grams digestible protein to megacalorie digestible energy (DE) ratio was 36.6, 35.9 and 32.4 for CBG, F-CBG and F-CBG + glucose, respectively. The decrease (36.6 to 32.4) resulted in increased nitrogen retained (P < .05), increased percentage nitrogen intake retained (P < .05) and increased percentage digested nitrogen retained (P < .01). Urinary nitrogen excretion was lower (P < .01) in wethers receiving the F-CBG + glucose treatment than in those receiving the F-CBG treatment (3.60 vs 5.09 g/day). Effects of formaldehyde treatment of CBG and rumen infusion of monensin on quantities of various crude protein (CP) components reaching the abomasum were also determined in a 4 X 4 Latin square design experiment. Total CP reaching the abomasum increased (P < .01) with formaldehyde treatment and resulted in increased quantities of amino acids reaching the abomasum (P < .05). Monensin had no effect on these two parameters, and no interaction between monensin and formaldehyde was observed. Dietary CP reaching the abomasum increased with formaldehyde treatment (P < .005) and with infusion of monensin (P < .025). Total microbial protein (MP) and MP reaching the abomasum per 100 g dry matter digested were not influenced by either formaldehyde or monensin.
mesh_terms: D000018:Abomasum / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D004032:Diet; D004063:Digestion / Q000187:drug effects; D005557:Formaldehyde / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D008715:Methionine / Q000378:metabolism / Q000494:pharmacology; D008985:Monensin / Q000494:pharmacology; D009584:Nitrogen / Q000378:metabolism*; D006109:Poaceae; D012417:Rumen / Q000187:drug effects / Q000378:metabolism*; D012756:Sheep / Q000378:metabolism*; D014935:Wool / Q000187:drug effects
chemical_list: D000596:Amino Acids; D005557:Formaldehyde; D008985:Monensin; D008715:Methionine; D005947:Glucose; D009584:Nitrogen