Comparison of immunofixation and immunoelectrophoresis methods in the identification of monoclonal immunoglobulins in serum.
1.A comparison has been made of the relative ability of immunofixation and immunoelectrophoresis to identify monoclonal IgG, IgA and IgM in three sera with high concentration and in three sera with low concentration of these proteins. 2. Immunofixation identified the proteins unambiguously in all six sera examined, whereas immunoelectrophoresis gave ambiguous results in one of the sera with high protein concentration and in three of the sera with low protein concentration. The superiority of immunofixation resulted from its greater resolution. It was condluded that immunofixation is the preferred technique for the identification of monoclonal immunoglobulins. 3. The sensitivity of immunofixation was greater at 40 degrees C than at 10 degrees C or 25 degrees C.
D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D007136:Immunoglobulins / Q000032:analysis / Q000302:isolation & purification*
D007136:Immunoglobulins