Binding of androgens to a nuclear-envelope fraction from the rat ventral prostate.
A nuclear-envelope fraction was isolated from the rat ventral prostate which is virtually free of DNA and contains little RNA or plasma membrane. Isolation of this nuclear-envelope fraction after incubation of purified nuclei with radioactive dihydrotestosterone results in labelling of the membrane. More binding of dihydrotestosterone is observed after incubations at 22 degrees C for 17 h than at 4 degrees C for 17 h or at 22 degrees C for 60 min. Scatchard analysis revealed a class of binding sites with KD 8.4 nM. Dihydrotesterone and testosterone were almost equally effective as competitors of labelled dihydrotestosterone binding on the purified nuclear-envelope fraction, whereas diethylstilboestrol was less effective and dexamethasone did not compete well. When the outer membrane of the nuclei was removed with Triton X-100, a 24% decrease in specific binding of androgens was observed. Castration 24 h before preparation of nuclei resulted in loss of the androgen binding to the membrane.
D000818:Animals; D002458:Cell Fractionation; D003907:Dexamethasone / Q000494:pharmacology; D004054:Diethylstilbestrol / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008854:Microscopy, Electron; D009685:Nuclear Envelope / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D011467:Prostate / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D013739:Testosterone / Q000494:pharmacology
D013196:Dihydrotestosterone; D013739:Testosterone; D004054:Diethylstilbestrol; D003907:Dexamethasone