Assay of HA-966 in rat plasma by capillary gas-liquid chromatography with nitrogen-selective detection.
A gas-liquid chromatographic method for the determination of the gamma-aminobutyric acid-like drug 1-hydroxy-3-aminopyrrolidone-2 (HA-966) in plasma is described. HA-966 was converted into its diacetyl derivative AC2HA-966 with acetic anhydride. This compound could be suitable eluted from a capillary OV-17 support-coated open tubular column. A sensitive detection method was achieved by making use of nitrogen-phosphorus-selective flame ionization.
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D008297:Male; D009584:Nitrogen / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism; D011760:Pyrrolidinones / Q000097:blood*; D051381:Rats
D011760:Pyrrolidinones; D010758:Phosphorus; C001369:1-hydroxy-3-amino-2-pyrrolidone; D009584:Nitrogen