Cycling in the idiotypical proliferative response of peripheral blood lymphocytes in normal and Salmonella typhi-immunized rabbits.
A partially inbred rabbit was immunized with repeated intravenous administrations of Salmonella typhi during a two year period, and three antiidiotypic sera were prepared against anti-S. typhi antibodies. An idiotype which was detectable by a heterologous precipitation reaction with one of the antiidiotypic sera and which was associated with anti-S. typhi antibodies was found using a radiobinding inhibition test in the normal serum from all rabbits examined in the colony, but not in any of unrelated rabbits. Peripheral blood lymphocytes from the idiotypic proband rabbit showed a cycling in the proliferative response with all the three antiidiotypic sera. The response with two of the three antisera subsided following repeated immunizations, with concomitant decline in the idiotype level in the serum, whereas the response with other serum was observed even after the lapse of 6 months after the repeated immunizations, the idiotype persisting in the serum. Peripheral blood lymphocytes from normal rabbits of the colony also showed a fluctuation in the proliferative response with the antiserum against the cross-reactive idiotype and expressed at intervals a suppressive activity on the proliferative response when mix-cultured with the responding lymphocytes in the presence of the antiidiotypic serum.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D000917:Antibody Formation; D007114:Immunization; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis*; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D010507:Periodicity; D011817:Rabbits; D012485:Salmonella typhi / Q000276:immunology*; D013936:Thymidine / Q000097:blood
D000907:Antibodies, Bacterial; D007130:Immunoglobulin Idiotypes; D013936:Thymidine