Crystallization and preliminary X-ray investigation of calmodulin.
Crystals of rat testis calmodulin, a multifunctional Ca2+-binding protein have been grown from solutions of 2-methyl-2,4-pentanediol. The crystals are triclinic, space group P1, with a = 29.79(4) A, b = 53.74(7) A, c = 24.78(3) A, alpha = 93.46(2)degrees, beta = 96.98(2)degrees, and gamma = 89.05(3)degrees. There is 1 calmodulin molecule per unit cell. The crystals are quite stable to x-rays and diffract beyond 2.5 A resolution.
D000818:Animals; D002135:Calcium-Binding Proteins / Q000302:isolation & purification; D002147:Calmodulin / Q000302:isolation & purification; D003460:Crystallization; D008297:Male; D011487:Protein Conformation; D051381:Rats; D013737:Testis / Q000032:analysis; D014961:X-Ray Diffraction
D002135:Calcium-Binding Proteins; D002147:Calmodulin