Membrane-associated ribosomes in producing and nonproducing mouse myeloma cells.
Mouse myeloma cell lines synthesize large amounts of immunoglobulin on their membrane-associated polyribosomes. Variants which no longer synthesize immunoglobulins have been studied and shown to have the same number of tightly bound membrane-associated polyribosomes as the parental cell lines. These polyribosomes are still active in the synthesis of nonimmunoglobulin proteins.
D000818:Animals; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D007136:Immunoglobulins / Q000096:biosynthesis*; D007425:Intracellular Membranes / Q000378:metabolism*; D051379:Mice; D009101:Multiple Myeloma; D011132:Polyribosomes / Q000378:metabolism*; D014176:Protein Biosynthesis; D012965:Sodium Chloride / Q000494:pharmacology
D007136:Immunoglobulins; D012965:Sodium Chloride; D003513:Cycloheximide