Enzymic determination of urinary free L-fucose.
Conditions necessary for the precise measurement of free L-fucose in urine by an enzymic method using L-fucose dehydrogenase have been studied. The normal urinary levels of L-fucose were 16.4 /+- 9.1 micrograms/ml in children (22.2 /+- 6.5 micrograms/mg of creatinine) and 17.7 /+- 8.5 micrograms/ml in adults (16.6  /+- 5.7 micrograms/mg of creatinine). There was a close correlation between the concentration of free L-fucose and that of creatinine. A close correlation was also found between the concentration of free L-fucose and alpha-L-fucosidase activity. Thus, it was suggested that the free L-fucose in urine reflected the metabolism of L-fucose or L-fucose-containing glycoconjugates. In a preliminary screening test, several urine samples which showed high concentrations of free L-fucose, namely cases of "fucosuria" were found.
D000328:Adult; D002237:Carbohydrate Dehydrogenases; D002648:Child; D003404:Creatinine / Q000652:urine; D005260:Female; D005643:Fucose / Q000652:urine*; D006801:Humans; D008297:Male; D008722:Methods; D012016:Reference Values; D005644:alpha-L-Fucosidase / Q000652:urine
D005643:Fucose; D003404:Creatinine; D002237:Carbohydrate Dehydrogenases; C020154:L-fucose dehydrogenase; D005644:alpha-L-Fucosidase