Retention of the 4-pro-R hydrogen atom of mevalonate at C-2,2' of bacterioruberin in Halobacterium halobium.
Intact cells of Halobacterium halobium fed with (3R,4R)-[2-14C,4-3H1]mevalonic acid were found to incorporate label into acyclic C40 and C50 carotenoids, of which bacterioruberin was the most abundant. The 14C/3H ratios of the isolated carotenoids demonstrated that the 4-pro-R hydrogen atom of mevalonic acid was retained at the C-2 and C-2' positions of bacterioruberin. Diphenylamine was found to inhibit the production of bacterioruberin.
D002250:Carbon Radioisotopes; D002338:Carotenoids / Q000096:biosynthesis*; D004159:Diphenylamine / Q000494:pharmacology; D006217:Halobacterium / Q000187:drug effects / Q000378:metabolism*; D008798:Mevalonic Acid / Q000378:metabolism*; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; C002951:bacterioruberin; D002338:Carotenoids; D004159:Diphenylamine; D008798:Mevalonic Acid