A new subfraction of routine fractionation.
The prothombin complex is adsorbed from plasma or cryoconcentrate supernatant onto DEAE-sephadex A50. The adsorbent is washed several times with 0.21 M NaCl. The first washing proved to be a new subfraction of routine fractionation suitable as starting material for the purification of several proteins such as C1-inactivator, N-carboxypeptidase, ceruloplasmin and kallikrein.
D000327:Adsorption; D001798:Blood Proteins / Q000302:isolation & purification*; D005591:Chemical Fractionation / Q000379:methods; D002852:Chromatography, Ion Exchange; D006801:Humans; D011516:Prothrombin
D001798:Blood Proteins; D011516:Prothrombin