Preliminary studies on the validity of in vitro measurement of drug toxicity using HeLa cells. II. Drug toxicity in the MIT-24 system compared with mouse and human lethal dosage of 52 drugs.
By a comparison of the 50% inhibitory concentration to HeLa cells in the microtitre Metabolic Inhibition Test supplemented by microscopy of cells after 24 h incubation (the MIT-24 test), of 52 drugs with their mouse i.v. LD50 and available approximate human lethal dosage, 7 were found to have a human LD considerably lower than HeLa IC50, indicating a lethal action to specialized functions of the human body not found in vitro, while 39 were found to have a gross similarity between the human LD and HeLa IC50.
D000818:Animals; D006367:HeLa Cells / Q000187:drug effects*; D006801:Humans; D007928:Lethal Dose 50; D051379:Mice; D004364:Pharmaceutical Preparations / Q000378:metabolism; D011485:Protein Binding; D014116:Toxicology / Q000379:methods*
D004364:Pharmaceutical Preparations