An improved electrophoretic method for a screening program for haemoglobinopathies.
A method for a screening program for haemoglobinopathies in a starch agar gel mixed with saponin is presented. Normal and abnormal blood containing haemoglobins S, C, I, M Boston, D Punjab, beta thalassaemia major and beta thalassaemia minor, were applied, in a tray with the capacity for 100 samples. The electrophoresis was performed in 45 min using 300 V. This method offers special advantages for the examination of a large number of samples, using a small amount of whole blood and without the previous preparation of haemoglobin solution.
D004587:Electrophoresis, Agar Gel / Q000379:methods; D006444:Hemoglobin C / Q000032:analysis; D006449:Hemoglobin M / Q000032:analysis; D006451:Hemoglobin, Sickle / Q000032:analysis; D006453:Hemoglobinopathies / Q000175:diagnosis*; D006454:Hemoglobins / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis; D012503:Saponins; D013789:Thalassemia / Q000175:diagnosis
D006451:Hemoglobin, Sickle; D006454:Hemoglobins; D006455:Hemoglobins, Abnormal; D012503:Saponins; D006444:Hemoglobin C; C026237:hemoglobin D Punjab; C026236:hemoglobin I, non-mammalian; D006449:Hemoglobin M