Human fibroblast strain with normal survival but abnormal postreplication repair after ultraviolet light irradiation.
Postreplication repair has been studied in ultraviolet light (UV)-irradiated fibroblast strains derived from eight apparently normal control donors and seven xeroderma pigmentosum patients. One control donor strain had an intermediate defect in postreplication repair similar to that in excision-deficient xeroderma pigmentosum fibroblasts. However, unlike the xeroderma pigmentosum strains, this control donor strain had normal UV-induced unscheduled DNA synthesis and normal survival after irradiation with UV. This unique fibroblast strain should after irradiation with UV. This unique fibroblast strain should be useful in studies designed to elucidate the possible role of postreplication repair in UV-induced carcinogenesis and mutagenesis.
D000293:Adolescent; D000328:Adult; D000368:Aged; D002455:Cell Division; D002460:Cell Line; D002675:Child, Preschool; D004247:DNA / Q000528:radiation effects*; D004260:DNA Repair; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000032:analysis; D005260:Female; D005347:Fibroblasts; D006801:Humans; D008297:Male; D008970:Molecular Weight; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics
D004277:DNA, Single-Stranded; D004247:DNA