Isolation of the newly synthesized DNA of HeLa cells containing beta-2'-deoxy-6-thioguanylate on an organomercurial agarose column.
It has been shown that beta-2'-deoxy-6-thioguanosine is incorporated into mammalian DNA via its triphosphate form. The newly synthesized DNA of HeLa cells containing thioguanosine, adsorbed specifically on an organomercurial agarose column and was eluted with 2-mercaptoethanol. By recycling this affinity chromatography, the newly synthesized DNA strands were separated successfully from non-adsorbable parental strands. This system may provide a new tool for the study of DNA replication.
D002846:Chromatography, Affinity / Q000379:methods; D004247:DNA / Q000096:biosynthesis / Q000302:isolation & purification*; D003848:Deoxyguanine Nucleotides / Q000378:metabolism*; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D009941:Organomercury Compounds; D013010:Sonication; D013873:Thionucleotides / Q000378:metabolism*
D003848:Deoxyguanine Nucleotides; D009941:Organomercury Compounds; D013873:Thionucleotides; C021173:2'-deoxy-6-thioguanosine 5'-triphosphate; D004247:DNA