Automated colorimetric estimation of glycosylated haemoglobins.
The measurement of glycosylated haemoglobin is now widely used as a guide to glycaemic control in patients with diabetes mellitus. Present methods for measurement of glycosylated haemoglobin are either laborious, expensive or both. We describe a precise and inexpensive method for the automated analysis of glycosylated haemoglobin based on a colorimetric technique. The within-batch coefficient of variation ranged from 2.0 to 4.8% and between-batch was less than 11.0%. At least 200 samples can be analysed per day.
D003124:Colorimetry / Q000379:methods; D003920:Diabetes Mellitus / Q000097:blood; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000032:analysis; D006027:Glycosides / Q000097:blood*; D006441:Hemoglobin A / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans
D006027:Glycosides; C008046:5-hydroxymethylfurfural; D006441:Hemoglobin A; D005662:Furaldehyde