A novel conformation of valinomycin in its barium complex.
Knowledge of the molecular mechanisms involved in ionophore-mediated cation transport would be valuable for understanding many essential functions of biological membranes. Cations are transported in several stages, such as formation of the ionophore-cation complex, diffusion across the cell membrane and subsequent release of the cation. Several conformational rearrangements are involved in this process, and so a detailed understanding of all the conformational possibilities of the ionophore seems to be essential for elucidating the molecular mechanism of ion transport. We are carrying out spectroscopic and crystallographic studies to explore the possible conformational stages of ionophores by complexing them, in different solvents, with cations of various sizes and charges. We report here a novel conformation of the ionophore valinomycin in its barium complex. It can be described as an extended depsipeptide chain, without interval hydrogen bonds, wound in the form of an ellipse with the two barium ions located at the foci.
D001464:Barium; D002942:Circular Dichroism; D007476:Ionophores; D008958:Models, Molecular; D011487:Protein Conformation; D014634:Valinomycin
D007476:Ionophores; D014634:Valinomycin; D001464:Barium