Nitrogen estimation in biological samples by use of chemiluminescence.
We describe an apparatus modified for the chemiluminescent estimation of nitrogen in biological and clinical samples. Analytical characteristics have been assessed and results compared with those by the traditional Kjeldahl method. The chemiluminescence method, much faster and more sensitive than the traditional method, is at least as accurate, precise, and reproducible. Costs are low, and the method should find a place in laboratories needing, for example, rapid assessments of nitrogen balance in surgical patients and renal function in patients with renal failure, or estimations of small amounts of specific nitrogen-containing chemical substances in biological samples.
D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D003124:Colorimetry / Q000379:methods; D006801:Humans; D008163:Luminescent Measurements; D008722:Methods; D009132:Muscles / Q000032:analysis; D009584:Nitrogen / Q000032:analysis* / Q000097:blood / Q000378:metabolism / Q000652:urine; D011506:Proteins / Q000032:analysis; D051381:Rats; D014508:Urea / Q000652:urine
D001798:Blood Proteins; D011506:Proteins; D014508:Urea; D009584:Nitrogen