The effect of the 3-hydroxy-3-methylglutaryl CoA reductase inhibitor ML-236b on phytosterol synthesis in Acer pseudoplatanus tissue culture.
Sycamore cell cultures were incubated with various labelled sterol precursors. ML-236B, a fungal metabolite, caused virtually total inhibition of acetate or leucine incorporation into sterols, while mevalonate incorporation was unaffected. Sterol synthesis from endogenous precursors, measured by incorporation of [Me-14C] methionine into the side chain, continued at a reduced rate for at least 6 h after addition of the inhibitor.
D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D007930:Leucine / Q000378:metabolism; D009183:Mycotoxins / Q000494:pharmacology*; D010840:Phytosterols / Q000096:biosynthesis*; D014197:Trees / Q000201:enzymology / Q000378:metabolism*
D002250:Carbon Radioisotopes; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D009183:Mycotoxins; D010840:Phytosterols; D007930:Leucine