Purification of bovine retinal pigment epithelial cells by dissociation in calcium free buffers and centrifugation in Ficoll density gradients followed by "recovery" in tissue culture.
D000818:Animals; D002118:Calcium; D002417:Cattle; D002452:Cell Count; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D010857:Pigment Epithelium of Eye / Q000166:cytology*
D003470:Culture Media; D002118:Calcium