[Rapid diagnosis of anaerobic infections by means of the direct gaschromatographic study of clinical materials].
Current methods for isolation and identification of obligately anaerobic bacteria are laborious and time-consuming. Direct gas-liquid chromatography of purulent and serous specimens for short-chain fatty acids allows a presumptive diagnosis of anaerobic infection. Good correlation was found for the cultural recovery of anaerobic bacteria and the presence of propionic acid in concentrations of greater than or equal to 1 muMol/ml, isobutyric, butyric, isovaleric, valeric, isocaproic and caproic acid at greater than or equal to 0.1 muMol/ml, and succinic acid at greater than or equal to 2 muMol/ml (either as single acids or in combination). Specimens yielding no anaerobic bacteria in culture contained only acetic, lactic and small amounts of succinic acid, but none of the volatile acids.
D000693:Anaerobiosis; D001424:Bacterial Infections / Q000175:diagnosis*; D002849:Chromatography, Gas; D005232:Fatty Acids, Volatile / Q000032:analysis*; D006801:Humans; D013492:Suppuration / Q000378:metabolism
D005232:Fatty Acids, Volatile
Schnelldiagnose anaerober Infektionen mit der direkten gaschromatographischen Untersuchung von klinischem Material.