The intracellular location of a Ca2+-stimulated phospholipase A1 in rat brain.
Rat brain contains a phospholipase A1 which hydrolyzes phosphatidylethanolamine with a pH optimum of 9.5. The enzyme is stimulated by CaCl2 and inhibited by sodium taurocholate and Triton X-100. The subcellular distibution of the enzyme is similar to that of lactate dehydrogenase indicating the location of the phospholipase in the soluble cytoplasm in vitro.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000201:enzymology*; D003593:Cytoplasm / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D005227:Fatty Acids / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008245:Lysophospholipase / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054466:Phospholipases A1; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology*; D013656:Taurocholic Acid / Q000494:pharmacology
D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D013656:Taurocholic Acid; D010740:Phospholipases; D010741:Phospholipases A; D054466:Phospholipases A1; D008245:Lysophospholipase; D002118:Calcium