Evolution of the amino acid substitution in the mammalian myoglobin gene.
Multivariate statistical analyses were applied to 16 physical and chemical properties of amino acids. Four of these properties; volume, polarity, isoelectric point (charge), and hydrophobicity were found to explain adequately 96% of the total variance of amino acid attributes. Using these four quantitative measures of amino acid properties, a structural discriminate function in the form of a weighted difference sum of squares equation was developed. The discriminate function is weighted by the location of each particular residue within a given tertiary structure and yields a numerical discriminate or difference value for the replacement of these residues by different amino acids. This resulting discriminate value represents an expression of the perturbation in the local positional environment of a protein when an amino acid substitution occurs. With the use of this structural discriminate function, a residue by residue comparison of the known mammalian myoglobin sequences was carried out in an attempt to elucidate the positions of possible deviations from the known tertiary structure of sperm whale myoglobin. Only 11 of the 153 residue positions in myoglobin demonstrated possible structural deviations. From this analysis, indices of difference were calculated for all amino acid exchanges between the various myoglobins. All comparisons yielded indices of difference that were considerably lower than would be expected if mutations had been fixed at random, even if the organization of the genetic code is taken into consideration. On the basis of these results, it is inferred that some form of selection has acted in the evolution of mammalian myoglobins to favor amino acid substitutions that are compatible with the retention of the original conformation of the protein.
D000595:Amino Acid Sequence; D000596:Amino Acids; D000704:Analysis of Variance; D000818:Animals; D005075:Biological Evolution; D055598:Chemical Phenomena; D002621:Chemistry; D005796:Genes; D006801:Humans; D008322:Mammals / Q000235:genetics*; D009154:Mutation; D009211:Myoglobin / Q000235:genetics*; D011487:Protein Conformation; D012641:Selection, Genetic; D012995:Solubility
D000596:Amino Acids; D009211:Myoglobin