Composition of hnRNA-associated proteins in rat liver is specifically altered after cycloheximide treatment.
Rats were treated with non-lethal doses of alpha-amanitin or cycloheximide. Nuclei were prepared and the particles carrying heterogeneous nuclear RNA (30--40 S-particles) isolated by density gradient centrifugation. In the case of alpha-amanitin the yield of particles was reduced to about 45%. Cycloheximide affected the composition of proteins associated with the nuclear RNA. In particular, the concentration of a 110 000 molecular weight protein as determined by sodium dodecylsulfate gel electrophoresis was reduced to 20--30% after 2 h and then increased to 150--180% of the control before it approached the normal level after 30 h. Possible mechanisms underlying these changes are discussed.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D009698:Nucleoproteins / Q000378:metabolism*; D012332:RNA, Heterogeneous Nuclear / Q000378:metabolism*; D051381:Rats; D012261:Ribonucleoproteins / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D000546:Amanitins; D009698:Nucleoproteins; D012332:RNA, Heterogeneous Nuclear; D012261:Ribonucleoproteins; D003513:Cycloheximide