title: Effect of various chemicals on macromolecular binding during oxidative dealkylation of dimethylnitrosamine by hamster liver microsomes.
abstract: Differential inhibitory effects of various chemicals on both formaldehyde formation and total macromolecular binding have been examined during oxidative demethylation of [14C]dimethylnitrosamine (DMN) by hamster liver microsomes. One group of chemicals such as diethyldithiocarbamate (DEDTC), aminoacetonitrile (AAN), 2-[(2,4-dichloro-6-phenyl) phenoxy]-ethylamine (DPEA), azide and ethanol inhibits both HCHO formation and total macromolecular binding. A second group of chemicals such as reduced glutathione, semicarbazide and N-(1-naphthyl)thiourea (NTU) inhibits macromolecular binding without appreciably affecting HCHO formation. Possible mechanisms of these differential inhibitory effects by these chemicals are discussed.
mesh_terms: D000818:Animals; D006224:Cricetinae; D003640:Dealkylation; D004128:Dimethylnitrosamine / Q000378:metabolism*; D005557:Formaldehyde / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008297:Male; D008647:Mesocricetus; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D011506:Proteins / Q000378:metabolism
chemical_list: D006657:Histones; D046911:Macromolecular Substances; D011506:Proteins; D005557:Formaldehyde; D009249:NADP; D004128:Dimethylnitrosamine