Purification and characterization of anti-cholinesterase factor from the venom of Naja naja atra.
The anti-cholinesterase factor from Naja naja atra venom was purified by means of successive column chromatography on Sephadex G-75, carboxymethyl-cellulose and hydroxyapatite. The purified anti-cholinesterase factor was homogeneous by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The molecular weight was estimated to be approx. 50 000. The isoelectric point was determined to be 6.8. This factor was found to be the first cholinesterase inhibitor protein. We have called this anti-cholinesterase factor atramine.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification*; D002850:Chromatography, Gel; D004546:Elapid Venoms / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008970:Molecular Weight; D011506:Proteins
D000596:Amino Acids; D002800:Cholinesterase Inhibitors; D004546:Elapid Venoms; D011506:Proteins; C026122:atramine