Characterization of the metallothioneins induced in HeLa cells by dexamethasone and zinc.
The metallothioneins induced in HeLa cells by dexamethasone and Zn2+ were labeled with [3H]cysteine and [35S]cysteine respectively. These labeled metallothioneins were compared to one another and to metallothioneins from human liver by (a) gel permeation chromatography on Sephadex G-75 columsn, (b) ion-exchange chromatograhy on DEAE-Sephadex A-25 columns, (c) electrophoresis on non-denaturing polyacrylamide gels, and (d) electrophoresis of reduced and alkylated proteins on dodecylsulfate/polyacrylamide gels. The dexamethasone-induced metallothioneins of HeLa cells, the Zn2+-induced metallothioneins of HeLa cells, and the metallothioneins of human liver were indistinguishable by these four criteria.
D002852:Chromatography, Ion Exchange; D003907:Dexamethasone / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism; D008667:Metalloproteins / Q000032:analysis*; D008668:Metallothionein / Q000032:analysis* / Q000096:biosynthesis; D015032:Zinc / Q000494:pharmacology*
D008667:Metalloproteins; D003907:Dexamethasone; D008668:Metallothionein; D015032:Zinc