title: Characterization of separated prostatic and phosphatase forms in normal and castrated rats.
abstract: We have investigated the effects of castration and androgen replacement on the kinetic characteristics of rat prostatic acid phosphatases (AP). Chromatography on DEAE-cellulose DE-23 and on Sephacryl S-200 allowed the separation of lysosomal (S-1) and secretory (S-3) forms of AP. In addition, these techniques revealed a third enzymic form (S-2), which eluted in the void volume of the Sephacryl S-200 column and which occurred in significant amounts only 15 days after castration. The S-2 form was extremely resistant (Ki 1500 microM) to L-tartrate inhibition in the 15-day post-castration rat which is in contrast to the behaviour of S-2 (Ki 145 microM) in control animals. The appearance of this unidentified AP form can explain the dramatic drop in the per cent inhibition of total rat AP observed between 7 and 15 days post-castration.
mesh_terms: D000135:Acid Phosphatase / Q000302:isolation & purification*; D000728:Androgens / Q000494:pharmacology; D000818:Animals; D002369:Castration; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D007700:Kinetics; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D011467:Prostate / Q000187:drug effects / Q000201:enzymology*; D051381:Rats
chemical_list: D000728:Androgens; D000135:Acid Phosphatase