Inhibition of ribulose-1,5-bisphosphate carboxylase-oxygenase activities by hydroxylamine.
Hydroxylamine directly and reversibly inhibits both activities of homogeneous ribulose-1,5-bisphosphate carboxylase-oxygenase (3-phospho-D-glycerate carboxy-lyase (dimerizing), EC 4.1.1.39) isolated from diverse sources. NH2OH is an uncompetitive inhibitor of carboxylase activity with respect to ribulose-bisphosph ate. This reagent also reacts non-enzymically with ribulosebisphosphate to deplete this substrate. Contrary to previous reports, these results indicate that hydroxylamine directly and indirectly inhibits both activities of this bifunctional enzyme.
D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D006898:Hydroxylamines / Q000494:pharmacology*; D007700:Kinetics; D010944:Plants / Q000201:enzymology*; D012273:Ribulose-Bisphosphate Carboxylase / Q000037:antagonists & inhibitors*; D012274:Ribulosephosphates / Q000037:antagonists & inhibitors; D013855:Thiobacillus / Q000201:enzymology*
D006898:Hydroxylamines; D012274:Ribulosephosphates; D002262:Carboxy-Lyases; D012273:Ribulose-Bisphosphate Carboxylase