The conformational properties of human plasma apolipoprotein C-II. A spectroscopic study.
The solution properties of human plasma apolipoprotein C-II (apoC-II) have been studied by analytical ultracentrifugation, circular dichroic spectroscopy, and fluorescence spectroscopy. ApoC-II self-associates in solution, even though no rigorous thermodynamic analysis of the mode of self-association could be established. The reversible denaturation of apoC-II by guanidinium chloride (GdmCl) proceeded in a sequential fashion. Initial disruption of protein self-association by 0.3 M GdmCl was followed by cooperative unfolding of monomeric protein at higher GdmCl concentratioans with a midpoint 1.1 M GdmCl. Based on tryptophan fluorescence quenching unfolded apoC-II was more permeable to penetration by small molecules than the self-associated protein. a very low free energy (delta GH2O = 2.8 kcal/mol) of denaturation was calculated from the GdmCl denaturation titration curve. Heating of apoC-II to 55 degrees C did not induce a reversible cooperative unfolding of the protein. Calculations, bsed on Chou-Fasman probability algorithms, reveal three sequential helical regions in apoC-II and one beta sheet structure (residues 61-74P. The locations of these regions are consistent with the known physiological functions of apoC-II.
D000595:Amino Acid Sequence; D053304:Apolipoprotein C-II; D001053:Apolipoproteins / Q000097:blood; D001056:Apolipoproteins C; D002942:Circular Dichroism; D019791:Guanidine; D006146:Guanidines; D006801:Humans; D006951:Hyperlipoproteinemias / Q000097:blood; D046911:Macromolecular Substances; D008970:Molecular Weight; D011487:Protein Conformation; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence
D053304:Apolipoprotein C-II; D001053:Apolipoproteins; D001056:Apolipoproteins C; D006146:Guanidines; D046911:Macromolecular Substances; D019791:Guanidine