Estrone beta-glucosidase activity in human placenta.
The 105 000g supernatant from human placental homogenates, prepared in the presence of sodium taurocholate and Cutscum, contained beta-glucosidase activity towards estrone glucoside as well as towards 4-methylumbelliferyl glucoside (4-MU-glucoside) and glucocerebroside. After partial purification, the estrone glucosidase was found to be active only after the addition of negatively charged phospholipid, whereas the other beta-glucosidases did not exhibit this requirement. The estrone glucosidase was separated from the 4-MU-glucosidase by chromatography on Sephadex G-200 with 0.1% sodium taurocholate in the eluting buffer. The estrone glucosidase was mainly contained in material with a pI of 4.7, while the 4-MU-glucosidase was distributed in fractions with pI values of 4.7 and 6.2 to 6.4. The partially purified estrone glucosidase had a pH optimum of 5.8, as distinct from that of 6.4 found for the 4-MU-glucosidase, and differed markedly from the 4-MU-glucosidase in its response to treatment with heat, sulfhydryl reagents, and detergents. Its sensitivity to changes in pH differed from those reported for glucocerebrosidase.
D003902:Detergents / Q000494:pharmacology; D004970:Estrone / Q000031:analogs & derivatives; D005260:Female; D005959:Glucosidases / Q000302:isolation & purification / Q000378:metabolism*; D005960:Glucosides; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006923:Hymecromone / Q000031:analogs & derivatives; D010743:Phospholipids / Q000494:pharmacology; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013379:Substrate Specificity; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D003902:Detergents; D005960:Glucosides; D010743:Phospholipids; D013439:Sulfhydryl Reagents; C014110:4-methylumbelliferyl glucoside; C025932:estrone glucoside; D004970:Estrone; D006923:Hymecromone; D005959:Glucosidases; C025933:estrone beta-glucosidase