title: Alkylation by 1,2:5,6-dianhydrogalactitol of deoxyribonucleic acid and guanosine.
abstract: DNA was alkylated in neutral solution at 37 degrees C with 1,2:5,6-dianhydrogalactitol and hydrolysed to yield two principal products, identified as 7-galactitylguanine and 1,6-dideoxy-1,6-di(guanin-7-yl)galactitol. The reaction products were separated by chromatography on Sephadex G-10 and Dowex 50 (H+ form). The two compounds were also obtained by reaction between dianhydrogalactitol and guanosine in acetic acid. The products were characterized from their u.v.-spectral data by comparison with those of the 7-alkylguanines and were also identified by mass spectrometry.
mesh_terms: D000478:Alkylation; D004247:DNA; D003961:Dianhydrogalactitol; D006151:Guanosine; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet; D013402:Sugar Alcohols
chemical_list: D013402:Sugar Alcohols; D006151:Guanosine; D003961:Dianhydrogalactitol; D004247:DNA