The separation and structure of infective trypanosomes from cultures of Trypanosomas brucei grown in association with tsetse fly salivary glands.
Infective trypanosomes developed when Trypanosoma brucei was cultivated at 28 C in a liquid medium containing tsetse fly head-salivary gland explants. They were separated from the noninfective culture forms using DEAE-cellulose column chromatography. It was demonstrated by light and electron microscopy that the separated organisms were morphologically similar to metacyclic stages found in a tsetse fly and that they had a characteristic surface coat. Single metacyclic trypanosomes isolated from the cultures gave rise to infections in mice.
D000818:Animals; D003470:Culture Media; D005407:Flagella / Q000648:ultrastructure; D051379:Mice; D012469:Salivary Glands / Q000469:parasitology; D014346:Trypanosoma brucei brucei / Q000302:isolation & purification / Q000472:pathogenicity* / Q000648:ultrastructure; D014370:Tsetse Flies / Q000469:parasitology
D003470:Culture Media