Determination of the K2 lipid clearance coefficient by centrifugation. A comparison with the Gordis test and spectrophotometric measurement.
The K2 lipid clearance coefficient was calculated for the intravenous fat tolerance test (iv FTT), after estimating triglycerides by spectrophotometry, the Gordis technique or by preparative ultracentrifugation. The best correlation between the K2 values (r = 0.95, p < 0.001) is for the results of the Gordis test and ultracentrifugation. Spectrophotometry shows poorer correlations: r = 0.66 and p < 0.05 with the Gordis test, and r = 0.78 and p < 0.01 with ultracentrifugation. Spectrophotometry is only suitable for detection but the two other techniques can be applied to more detailed studies. Ultracentrifugation is faster, less laborious and less liable to error.
D002499:Centrifugation, Density Gradient / Q000379:methods; D005217:Fat Emulsions, Intravenous / Q000378:metabolism*; D005260:Female; D006801:Humans; D007700:Kinetics; D013057:Spectrum Analysis / Q000379:methods; D014280:Triglycerides / Q000097:blood / Q000378:metabolism; D014461:Ultracentrifugation / Q000379:methods
D005217:Fat Emulsions, Intravenous; D014280:Triglycerides