Are findings with isolated rat livers after short calcium free perfusion relevant for isolated cells?
In the isolated liver transient perfusion without calcium results in cholestasis which was characterized by an increased efflux rate across the sinusoidal membrane and inhibition of the concentrative transport of bromosulfophthalein to the canalicular side of the cell. Cholestasis could not be reversed within the usual duration of liver perfusion.
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002779:Cholestasis / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D066298:In Vitro Techniques; D007444:Inulin / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D013448:Sulfobromophthalein; D013656:Taurocholic Acid / Q000378:metabolism; D013997:Time Factors
D013448:Sulfobromophthalein; D013656:Taurocholic Acid; D007444:Inulin; D005978:Glutathione; D002118:Calcium