H-2 analyses of blastocyst-derived cell lines from mice bearing T and tw18 mutations.
T/T and tw18/tw18 blastocysts appear to survive in culture for periods of 3 weeks at frequencies equal to that of normal embryos. We have, therefore, undertaken to generate cell lines with a homozygous mutant genotype. We attempted to identify these cells by H-2 haplotype analysis. The results indicate that barring recombination, which would be expected to occur at a frequency of about 15%, 1 of 10 blastocyst-derived cell lines from a (+/tw18 x +/tw18) cross had a tw18/tw18 genotype and 1 of 4 lines from a (T/+ x T/+) cross had a T/T genotype. Unexpectedly, all of the remaining lines were heterozygous; no +/+ lines were observed. The putative homozygous mutant lines might be useful in attempts to characterize tw18-specific antigens.
D000042:Absorption; D000818:Animals; D001755:Blastocyst / Q000276:immunology*; D002460:Cell Line; D003602:Cytotoxicity, Immunologic; D005260:Female; D006183:H-2 Antigens / Q000235:genetics*; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains / Q000276:immunology*; D011247:Pregnancy
D006183:H-2 Antigens