Large-scale isolation of UV-sensitive clones of CHO cells.
We have isolated 54 ultraviolet light (UV) sensitive clones of Chinese hamster ovary (CHO) cells, including two from a parent cell line which is hypersensitive to ethyl methanesulfonate (EMS) and is also sensitive to X rays. A replica plating technique was used for the isolation of two of the clones, and a semiautomated technique was used for the isolation of the other 52 clones. We have observed UV sensitization of up to 5-fold in the mutants relative to the parent in terms of the slopes of the survival curves. Seven of the clones were examined for DNA repair competence using a repair replication assay, and all exhibited a DNA repair defect resembling that seen in human mutant xeroderma pigmentosum cells. We have also demonstrated an approximately 9-fold enhancement in the UV mutagenic response of two of the repair replication-defective clones relative to the parent for resistance to ouabain, 6-thioguanine, and 8-azaadenine.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002999:Clone Cells / Q000528:radiation effects*; D006224:Cricetinae; D003412:Cricetulus; D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D007621:Karyotyping; D009154:Mutation; D010641:Phenotype; D012641:Selection, Genetic; D013997:Time Factors; D014466:Ultraviolet Rays
D005020:Ethyl Methanesulfonate