In vitro development of Trichostrongylus colubriformis, from infective larvae to young adults.
Trichostrongylus colubriformis infective larvae, stimulated to exsheath with a 70-min ex-sheathing process, completed exsheathment and developed to young adult males and females in four different, two-step, roller-culture systems in 16 to 27 days. Complex, cell-free medium RFN-2 was used in the exsheathing process and in Step 1 cultures. Complex, cell-free medium API-16 was used, alone or supplemented with pepsin or glutathione (reduced), or both, as the Step 2 culture. Development to young adults was optimal when larvae were incubated in medium RFN-2 for 2 days and in medium API-16 supplemented with pepsin for 21 or 28 days and with glutathione for the first 8 days. The largest young adult male and female were 1.75 and 2.83 mm long, respectively. This system produced maximal yields of less than 1% young adults in populations of about 100,000 T. colubriformis by days 16 to 28.
D000818:Animals; D003470:Culture Media; D007814:Larva / Q000254:growth & development; D009024:Morphogenesis; D013997:Time Factors; D014251:Trichostrongyloidea / Q000254:growth & development*
D003470:Culture Media