title: Incorporation of 3H-oleic acid by the proximal convoluted tubule cells of the chick (Gallus domesticus). Electron microscopic autoradiographic study.
abstract: Lipid metabolism in the cells of the renal proximal convoluted tubules (PCT) was investigated in healthy fowls and in fowls with the Fatty Liver and Kidney Syndrome (FLKS). The tissue was fixed at 10-25 min intervals after intravenous injection of 3H-oleic acid. The distribution of autoradiographic grains was analysed by the "circle method". In normal cells most of the silver grains were associated with the cytoplasmic organelles. Lipid droplets and Golgi elements had the highest specific activity relative to the nuclear activity, which was little above background level. Lysosome-like bodies and mitochondria had lower values. In the cells of the FLKS-affected birds a large porportion of the grains was located over the lipid droplets, which are abundant in this condition. The specific activity of the cytoplasmic organelles was barely 2-fold higher than the nuclear activity. The results suggest that there is a diminished incorporation of esterified fatty acids by the organelles of these cells and that the excess is transferred to the lipid droplets. The identity of low electron density particles observed in the PCT cell of severely affected birds is discussed.
mesh_terms: D000818:Animals; D002645:Chickens / Q000033:anatomy & histology*; D007674:Kidney Diseases / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D007687:Kidney Tubules, Proximal / Q000378:metabolism* / Q000648:ultrastructure; D009829:Oleic Acids / Q000378:metabolism*; D009940:Organoids / Q000378:metabolism; D011201:Poultry Diseases / Q000378:metabolism* / Q000473:pathology
chemical_list: D009829:Oleic Acids