Investigation of vinca alkaloid-plasma membrane interactions by detergent gel chromatography.
Detergent gel chromatography was successfully applied for the separation of protein subunits and lipid micelles of [14C]Vincristine-treated and sodium dodecyl sulphate-solubilized liver cell plasma membranes. The elution profiles of solubilized membranes depended on the pore size of the commercial agarose and agarose-polyacrylamide gels used. It was found that most of the membrane-bound Vincristine was associated with the solubilized lipid micelles and only a very small proportion was bound to protein subunits.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D002850:Chromatography, Gel / Q000379:methods*; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D011485:Protein Binding; D011506:Proteins / Q000032:analysis; D051381:Rats; D014748:Vinca Alkaloids / Q000378:metabolism*; D014750:Vincristine / Q000032:analysis
D008055:Lipids; D011506:Proteins; D014748:Vinca Alkaloids; D014750:Vincristine