Inhibition of beta-sitosterol on intestinal cholesterol absorption in rat using in vivo dual isotope ratio method.
The inhibitory effects of beta-sitosterol on intestinal cholesterol absorption were studied by means of a dual isotope plasma ratio method (in vivo), which is a new technique for the measurement of cholesterol absorption, as well as a ligated-loop method (in situ). The results obtained were as follows: 1. The absorption of beta-sitosterol itself was significantly less than cholesterol. Cholesterol was selectively absorbed from rat intestine. 2. When 100 to 1,000 microgram of beta-sitosterol were added to the dose solution containing 10 microgram of cholesterol, cholesterol absorption by the in vivo experiment decreased with the increae of additional beta-sitosterol. 3. A similar inhibitory effect of beta-sitosterol was observed by the in situ ligated-loop method. These results suggest that beta-sitosterol actually inhibits cholesterol absorption in the physiological state of an animal.
D000818:Animals; D002250:Carbon Radioisotopes; D002784:Cholesterol / Q000378:metabolism*; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000378:metabolism; D051381:Rats; D012855:Sitosterols / Q000378:metabolism / Q000494:pharmacology*; D014316:Tritium
D002250:Carbon Radioisotopes; D012855:Sitosterols; D014316:Tritium; D002784:Cholesterol