[Kinetic and regulatory properties of alpha-ketoglutarate dehydrogenase complex from bovine adrenals].
The dependence of the rate of reactions catalyzed by the alpha-ketoglutarate dehydrogenase complex on the alpha-ketoglutarate, CoA and NAD+ concentration is described by Michaelis-Menten equation. Km is 0.190, 0.012 and 0.025 mM, respectively. ADP plays a role of a complex activator at low alpha-ketoglutarate concentrations. ATP, on the contrary, produces a mixed type inhibition on the alpha-ketoglutarate dehydrogenase complex with respect to CoA and NAD+. A conclusion is drawn that the adrenal alpha-ketoglutarate complex regulation by ATP/ADP ratio value is possible.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000311:Adrenal Glands / Q000201:enzymology*; D000818:Animals; D002417:Cattle; D003065:Coenzyme A; D004789:Enzyme Activation; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism*; D007658:Ketone Oxidoreductases / Q000378:metabolism*; D007700:Kinetics; D009243:NAD
D009243:NAD; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D007658:Ketone Oxidoreductases; D007655:Ketoglutarate Dehydrogenase Complex; D003065:Coenzyme A
Kineticheskie i reguliatornye svoĭstva al'fa-ketoglutaratdegidrogenaznogo kompleksa, vydelennogo iz nadpochechnikov byka.