A stable isotope assay for pseudoracemic warfarin from human plasma samples.
A pseudoracemic technique utilizing a seable isotope in one enantiomer was employed for the simultaneous determination of (R) and (S)-warfarin from plasma of human subjects. The assay includes high performance liquid chromatographic clean-up prior to mass spectral analysis to eliminate ion interferences from either co-administered drugs or contamination of the source. The assay is reliable, accurate and precise to within 5% at the submicrogram level.
D002247:Carbon Isotopes; D002851:Chromatography, High Pressure Liquid; D003903:Deuterium; D006207:Half-Life; D006801:Humans; D013058:Mass Spectrometry; D013237:Stereoisomerism; D014859:Warfarin / Q000097:blood*
D002247:Carbon Isotopes; D014859:Warfarin; D003903:Deuterium