Early effects of ethylnitrosourea on the LDH isozyme of rat fetal central nervous system.
The sequential changes in isozyme distribution of lactate dehydrogenase and calculated ratios of H- to M-subunit were studied by means of acrylamide gel enzymoelectrophoresis in Wister rat fetal brains from pregnant rats which were administered ethylnitrosourea with a dose of 75 mg/kg on day 14 of gestation, to search for early effects on rat fetal brains of ENU which was transplacental carcinogen and teratogen, from a view point of metabolic aspects. The isozyme patterns were compared to those obtained from normal rat fetal brain. The H/M ratios of ENU-treated group were significantly lower than those of control group. These data suggested that retarded differentiation of rat fetal CNS was induced by administration of ENU as far as LDH was concerned. The present data were discussed in relation to teratogenesis and carcinogenesis.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D004622:Embryo, Mammalian; D005038:Ethylnitrosourea / Q000633:toxicity*; D005260:Female; D005333:Fetus; D005865:Gestational Age; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D009607:Nitrosourea Compounds / Q000633:toxicity*; D051381:Rats
D007527:Isoenzymes; D009607:Nitrosourea Compounds; D007770:L-Lactate Dehydrogenase; D005038:Ethylnitrosourea