High-performance anion-exchange chromatography of ultraviolet-absorbing constitutents of human urine.
A 100-microliters urine sample was chromatographed on a column packed with a strongly basic macroreticular anion-exchange resin (Diaion CDR-10,5--7 micrometers with a nominal 35% cross linkage). The elution was performed with a linear acetate gradient from 0 to 6.0 M at an average flow-rate of 0.72 ml/min and at an average pressure of 104 kg/cm2. The relative standard deviation of retention times and peak height was +/- 4% or less. The properties of the macroreticular anion-exchange resin, the effect of the particle size, the pH of acetate buffers, and the effect of the flow-rate of the eluent on the separation were investigated. Thirty three components of urine were then resolved and named.
D000837:Anion Exchange Resins / Q000592:standards; D001565:Benzoates / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D010649:Phenylalanine / Q000652:urine*; D011687:Purines / Q000652:urine*; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014364:Tryptophan / Q000652:urine*; D014574:Uronic Acids / Q000652:urine*
D000837:Anion Exchange Resins; D001565:Benzoates; D011687:Purines; D014574:Uronic Acids; D010649:Phenylalanine; D014364:Tryptophan