Composition and size distribution of bovine casein micelles.
Chromatography of glutaraldehyde-fixed skim-milk on controlled-pore glass (CPG-10, 300 nm) gave three micellar fractions whose averaged diameters, measured by electron microscopy, decreased progressively with increasing elution volume. Casein micelles with diameters up to 680 nm were detected. The casein composition of the same fractions from unfixed skim-milk was determined. As the fraction elution volume increased, kappa-casein varied from 7.7 to 11.4% of total casein, giving alpha s/kappa ratios of 6.1, 4.7 and 3.3. A plot of kappa-casein content versus micelle surface-to-volume ratio for skim-milk and the column fractions approximated to a straight line. Re-calculation of the published results from two other studies also gave linear relationships between kappa-casein content and surface area for artificial micelles. The three regression lines thus obtained had small intercepts. It was concluded that the data indicated the same fundamental structure for casein micelles, with a predominant surface location for kappa-casein, whether the micelles are natural or artificial and whether they are aggregated by Ca2+ alone or by Ca2+ together with calcium phosphate-citrate complex.
D000818:Animals; D002364:Caseins / Q000032:analysis*; D002417:Cattle; D003102:Colloids; D005260:Female; D008823:Micelles; D008892:Milk / Q000502:physiology*
D002364:Caseins; D003102:Colloids; D008823:Micelles