Specific progesterone receptors in hepatic microsomes of the female rat.
Microsomes isolated from the liver of female rats bind [3H] progesterone. The progesterone binding is highly specific, estradiol, cortisol, testosterone, 5 alpha-pregnane-3 beta ol-20-one or 16 alpha-hydroxyprogesterone show very low affinity. The binding is sulfhydryl group dependent, p-hydroxymercury benzoate or N-ethylmaleimide inhibit progesterone-microsome association but the inhibition can be partially reversed by the addition of glutathione. Progesterone-microsomal receptor complex formation is altered by Ca2+ and Mg2+, these cations block progesterone binding in concentration ranging from 2 to 5 mmol.
D000818:Animals; D001665:Binding Sites; D005260:Female; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011980:Receptors, Progesterone / Q000187:drug effects / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000494:pharmacology
D011506:Proteins; D011980:Receptors, Progesterone; D013438:Sulfhydryl Compounds; D011374:Progesterone