[Alteration of the permeability and composition of liver cell lysosomal membranes after a thermal burn].
Rat hepatocyte fractions enriched with lysosomes were studied one hour and 7 days after vast thermal burn of the skin. The ratio of free catepsine D activity to its total activity served as an index of lysosomal membrane resistance. The lipid composition was assayed by thin layer chromatography on silica gel. Material increase in lysosomal membrane permeability was recorded both one hour and 7 days after burn. Significant shifts were also seen in the composition of neutral lipids and phospholipids. Burn gave rise to the triglyceride fraction that was not present in the control. Among phospholipids the most labile were minor fractions, that is those of sphyngomyelin, phosphatidylinosite and phosphatidylserine. The changes in the lipids are considered as essential for destructive alterations in the liver in burn disease.
D000818:Animals; D002056:Burns / Q000378:metabolism*; D002403:Cathepsins / Q000378:metabolism; D007425:Intracellular Membranes / Q000032:analysis; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008247:Lysosomes / Q000378:metabolism*; D008563:Membrane Lipids / Q000032:analysis*; D010539:Permeability; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D013997:Time Factors
D008563:Membrane Lipids; D010743:Phospholipids; D002403:Cathepsins
Izmeneniia pronitsaemosti i sostava lizosomal'nykh membran kletok pecheni posle termicheskogo ozhoga.