Influence of uremic middle molecules on blood cells.
Uremic middle molecular weight substances are obtained from the ultrafiltrate of plasma of uremic patients. Seven subfractions are isolated by chromatography using Sephadex G-15 and DEAE 25 A columns. Each subfraction is tested for its inhibitory effect upon lymphocyte blastogenesis and influence upon osmotic fragility of red blood cells. Consequently, subfractions c and d exert notable effects on both lymphocytes and red blood cells. The main constituents of subfraction d are aspartic acid, glycine, glutamine, and cysteine. It is concluded that uremic middle molecular substances have a deleterious effect upon blood cells. Therefore, hemofiltration, which is advantageous for the removal of middle molecular weight substances, is considered to be a method of choice for uremic patients.
D000596:Amino Acids / Q000032:analysis; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*; D009996:Osmotic Fragility / Q000187:drug effects; D014118:Toxins, Biological / Q000032:analysis / Q000097:blood / Q000494:pharmacology; D014462:Ultrafiltration; D014511:Uremia / Q000097:blood*
D000596:Amino Acids; D014118:Toxins, Biological