Development of acetylcholine sensitivity of embryonic chick atria in culture.
Atria were isolated from chick embryos of 4--12 days incubation and maintained up to 9 days in culture to examine change in their sensitivity to acetylcholine (ACh). Beating of atria from 4-day embryos was not affected by ACh at the start but was inhibited after a few days of culture. Atria from old embryos which had been sensitive to ACh remained so in culture. Desensitization to ACh was marked in short-term culture of 4-day embryonic atria. Sera and embryo extracts facilitated the appearance of ACh sensitivity. Binding of quinuclidinyl benzilate was observed in ACh-insensitive atria. Cycloheximide, puromycin and actinomycin D prevented the appearance and maintenance of ACh sensitivity in culture, leaving the heartbeat unaffected.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002642:Chick Embryo; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D006321:Heart / Q000196:embryology*; D006325:Heart Atria / Q000187:drug effects / Q000378:metabolism; D006339:Heart Rate / Q000187:drug effects; D011691:Puromycin / Q000494:pharmacology; D011813:Quinuclidinyl Benzilate / Q000378:metabolism
D003609:Dactinomycin; D011691:Puromycin; D011813:Quinuclidinyl Benzilate; D003513:Cycloheximide; D000109:Acetylcholine