title: The effect of prostaglandins on sulphate and thymidine incorporation in pig cartilage.
abstract: Prostaglandins A1, A2, B2, E2, and F2 alpha inhibit glycosaminoglycan sulphation in pig costal cartilage discs above 0.1 micrometer M (5-25 ug/ml); no effects were found at concentrations down to 25 pg/ml. At concentrations above 1 microgram/ml (0.02 mM) prostaglandin E2 and F2 alpha inhibited cartilage DNA synthesis. However at 100 ng/ml (10 microM) this inhibition was again observed. The basal production of prostaglandins E2 and F2 alpha by the cartilage discs after incubation was below 15 pg/ml. The exact concentrations at which these inhibitory effects were observed varied from experiment (i.e. animal) to experiment.
mesh_terms: D000818:Animals; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D011453:Prostaglandins / Q000494:pharmacology*; D011454:Prostaglandins A / Q000494:pharmacology; D011456:Prostaglandins B / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D013431:Sulfates / Q000378:metabolism*; D013552:Swine; D013936:Thymidine / Q000378:metabolism*
chemical_list: D006025:Glycosaminoglycans; D011453:Prostaglandins; D011454:Prostaglandins A; D011456:Prostaglandins B; D011458:Prostaglandins E; D011460:Prostaglandins F; D013431:Sulfates; D004247:DNA; D013936:Thymidine