title: Synthesis and preliminary binding studies of 4,4-ditritio-(-)-nicotine of high specific activity.
abstract: 4,4-Ditritio-(-)-nicotine (5) of high specific activity (4.7 Ci/mmol) has been synthesized from (-)-nicotine via the readily prepared 4,4-dibromocotinine (3). Scatchard analysis of the binding of 5 to the crude mitochondrial fraction of whole rat brain revealed a Ka of 4.7 X 10(6) M-1 and 13 fmol of binding sites/mg of protein.
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D066298:In Vitro Techniques; D007553:Isotope Labeling / Q000379:methods; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D009538:Nicotine / Q000138:chemical synthesis* / Q000378:metabolism; D051381:Rats; D013237:Stereoisomerism; D014316:Tritium
chemical_list: D014316:Tritium; D009538:Nicotine