Quantitation of cis versus trans regulation of mouse beta-glucuronidase. Action of alleles of the Gus-r locus determining androgen inducibility.
Genetic studies have shown that the induction of beta-glucuronidase in mouse kidney in response to androgens is under the control of the Gus-r gene, closely linked to the beta-glucuronidase structural gene, Gus-s, on mouse Chromosome 5. Despite the fact that a single structural gene codes for beta-glucuronidase, enzyme molecules show considerable charge heterogeneity, presumably as a result of post-translational events. Because the enzyme is a tetramer, this heterogeneity has complicated examination of the cis versus trans action of the Gus-r gene. In order to resolve the problem, we have developed a quantitative method for determining the amount of beta-glucuronidase derived from each structural allele in mice heterozygous for beta-glucuronidase electrophoretic mobility. The method involves separation and quantitation of genetically variant cyanogen bromide peptides from the enzyme. Using this technique, we show that Gus-r acts cis, suggesting that it directly affects the template activity of the glucuronidase structural gene.
D000483:Alleles; D000818:Animals; D003433:Crosses, Genetic; D004790:Enzyme Induction; D005260:Female; D005796:Genes; D005966:Glucuronidase / Q000096:biosynthesis* / Q000235:genetics; D006579:Heterozygote; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D008815:Mice, Inbred Strains; D013045:Species Specificity; D013739:Testosterone / Q000494:pharmacology*
D013739:Testosterone; D005966:Glucuronidase