The effect of protein concentration on the binding of sulfamethoxypyridazine by human and bovine serum albumin.
Several methods of analyzing data obtained for ligand-macromolecule interactions at various concentrations of protein have been examined. Of the methods examined, only the constants ni, the number of binding sites of class i, and ki, the association constant for the i'th class of binding sites, were found useful in the detection of protein concentration dependent binding. The values of niki and sigma niki were calculated from previously obtained data for the interaction of sulfamethoxypyridazine with human and bovine serum albumin at seven protein concentrations. In disagreement with a previous report, the results obtained suggest that this protein-ligand interaction is either independent of protein concentration or that the protein concentration dependence of the binding constants is small relative to the experimental error involved in their determination.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007700:Kinetics; D008433:Mathematics; D012709:Serum Albumin / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013421:Sulfamethoxypyridazine / Q000378:metabolism*
D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D013421:Sulfamethoxypyridazine