Gas-liquid chromatographic determination of toxic diamines in permanent hair dyes.
A rapid, simple and sensitive analytical method has been developed for the simultaneous determination of 1,4-diaminobenzene, 2,5-diaminotoluene and 2,4-diaminoanisole in permanent (oxidation type) hair dyes. The method utilizes an ethyl acetate extraction in the presence of NaCl followed by direct injection into a gas liquid chromatograph equipped with a flame-ionization detector. The detection limits are 5 ng/microliter each for 1,4-diaminobenzene and 2,5-diaminotoluene and 20 ng/microliter for 2,4-diaminoanisole. The relative standard deviations at 5 tims the detection limits are 5.6, 5.8 and 4.6% for these three compounds, respectively. Recovery of diamines from "spiked" dyes was generally found to be greater than 85%.
D002273:Carcinogens; D002849:Chromatography, Gas / Q000379:methods; D003959:Diamines / Q000032:analysis* / Q000633:toxicity; D006202:Hair Dyes / Q000032:analysis*; D006203:Hair Preparations / Q000032:analysis*; D006801:Humans; D009784:Occupational Diseases / Q000517:prevention & control
D002273:Carcinogens; D003959:Diamines; D006202:Hair Dyes; D006203:Hair Preparations