An assay for RNA polymerase activity in rat brain nuclei: effects of injnection of oestradiol benzoate.
A sensitive, reliable assay of activity of RNA polymerase in purified nuclei from the rat brain is described. The assay measured the incorporation of [3H]guanosine triphosphate into RNA by nuclei. Little incorporation occurred in the presence of alpha-amanitin (400 ng) suggesting that RNA polymerase B activity was being measured. Preliminary evidence showed that after administration of oestradiol benzoate to 18-day-old female rats RNA polymerase activity was raised in areas of brain known to contain oestrogen receptors.
D000818:Animals; D001921:Brain / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D002458:Cell Fractionation / Q000379:methods; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006160:Guanosine Triphosphate / Q000378:metabolism; D051381:Rats
D004958:Estradiol; D006160:Guanosine Triphosphate; D012321:DNA-Directed RNA Polymerases