[Preparation and investigation of some properties of influenza virus nucleoid].
Influenza virus nucleoid ("core") was obtained by the treatment of virions with hydrolytic enzymes without the use of fixing agents. The "core" buoyant density in sucrose density gradient was 1.24 g/cm3. The subvirus particles are completely devoid of surface proteins, glycoproteins, and their polypeptide composition is represented by group P proteins; NP and M proteins. RNA in the core is insensitive to the effect of RNase. Morphological analysis by electron microscopy revealed the presence of spherical compact particles without spikes on their surface.
D000818:Animals; D002642:Chick Embryo; D002918:Chymotrypsin / Q000494:pharmacology; D006023:Glycoproteins / Q000032:analysis; D009980:Influenza A virus / Q000032:analysis / Q000187:drug effects / Q000302:isolation & purification / Q000648:ultrastructure*; D010455:Peptides / Q000032:analysis / Q000145:classification; D010740:Phospholipases / Q000494:pharmacology; D012367:RNA, Viral / Q000378:metabolism; D012260:Ribonucleases / Q000494:pharmacology; D014764:Viral Proteins / Q000032:analysis / Q000145:classification; D014771:Virion / Q000648:ultrastructure
D006023:Glycoproteins; D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins; D010740:Phospholipases; D012260:Ribonucleases; D002918:Chymotrypsin
Poluchenie i izuchenie nekotorykh svoĭstv nukleoida virusa grippa.