Analysis of methotrexate by isotachophoresis.
This paper shows that the anion of methotrexate (MTX) can be readily separated and quantified by isotachophoresis. An extraction method for MTX is also presented, appropriate for isotachophoretic studies. The extraction of MTX is based on the complexation and precipitation of MTX with metal ions. The recovery of MTZ from plasma is about 75%.
D000838:Anions; D001798:Blood Proteins / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008727:Methotrexate / Q000097:blood* / Q000302:isolation & purification; D012835:Silver Nitrate; D014238:Trichloroacetic Acid
D000838:Anions; D001798:Blood Proteins; D002712:Chlorides; D014238:Trichloroacetic Acid; D012835:Silver Nitrate; D008727:Methotrexate