A calibration procedure for the determination of alkaline phosphatase with the autoanalyzer.
We describe a kinetic procedure using the LKB reaction rate analyser for the calibration of an AutoAnalyzer alkaline phosphatase method. Measurements were made at zero incubation time with a special lid for the thermostated tunnel of the LKB reaction rate analyzer using assay conditions appropriate to the AutoAnalyzer method. The molar absorptivity of p-nitrophenol was estimated on the LKB reaction rate analyser used for assay of the enzyme standards. Other variables affecting the accuracy of the measurement were also determined.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D001322:Autoanalysis / Q000592:standards*; D002138:Calibration / Q000592:standards; D004796:Clinical Enzyme Tests / Q000295:instrumentation; D006801:Humans; D007700:Kinetics
D000469:Alkaline Phosphatase