Characterization of two DNA polymerases from cauliflower inflorescence.
Two distinct DNA polymerases, A and B, isolated from a rapidly growing apical tissue of cauliflower (Brassica oleracea var. botrytis) inflorescence have been characterized, and compared with DNA polymerases, alpha and beta, from mouse myeloma. Polymerase-A bears a strong resemblance to polymerase-alpha from mammalian cells in all properties examined. The character of polymerase-B is also quite similar to polymerase-beta of mammalian cells in chromatographic elution properties, template-primer utilization, sensitivity to inhibitors, response to KCl or KPi concentration, and other requirements for maximal activity, although it has a higher molecular weight (approx. 78,000) even in the presence of 0.25 M KCl that polymerase-beta (mol. wt. less than or equal to 50,000) of mammalian cells. This type of DNA polymerase has not been reported to exist in the plant system.
D000818:Animals; D004256:DNA Polymerase I / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D010944:Plants / Q000201:enzymology*; D013045:Species Specificity; D013698:Templates, Genetic
D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase