Rapid presumptive identification of anaerobes in blood cultures by gas-liquid chromatography.
Production of volatile and nonvolatile metabolic acids in blood culture broths by aerobic, facultative anaerobic, and obligate anaerobic bacteria was analyzed by gas-liquid chromatography. Anaerobic blood culture isolates were presumptively identified by the qualitative analysis of volatile fatty acids. Isolates, with a characteristic Gram stain reaction and cellular morphology, were identified by the following acid patterns: Bacteriodes fragilis group with acetic and propionic acids; Fusobacterium with acetic, butyric, and usually propionic acids; Veillonella with acetic and propionic acids; gram-positive cocci with acetic and butyric acids; and Clostridium with acetic and butyric acids.
D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification* / Q000378:metabolism; D001441:Bacteroides fragilis / Q000378:metabolism; D001769:Blood / Q000382:microbiology*; D002849:Chromatography, Gas; D003013:Clostridium / Q000378:metabolism; D005232:Fatty Acids, Volatile / Q000032:analysis* / Q000096:biosynthesis; D005673:Fusobacterium / Q000378:metabolism; D006801:Humans; D013045:Species Specificity; D014678:Veillonella / Q000378:metabolism
D005232:Fatty Acids, Volatile