Measurement of fat absorption using [3H]glycerol triether and [14C]glycerol trioleate in man.
A direct test of lipid absorption has been developed using [3H]glycerol triether as a lipid marker and [14C]glycerol trioleate as the test lipid. Percentage absorption of triolein is calculated from the isotope ratio ingested and excreted in faeces. The test is independent of a complete faecal collection, reproducible and shows little day to day variation. Absorption in 25 normal subjects ranged from 96.4--100% (mean 98.6, S.D. +/- 1.15). In 24 patients with known pancreatic or small intestinal disease and steatorrhoea absorption ranged from 0.01% to 89.9%. The test has also been assessed by comparison with a standard Lundh test and faecal fat estimation in 34 patients.
D000328:Adult; D000368:Aged; D002250:Carbon Radioisotopes; D002446:Celiac Disease / Q000378:metabolism; D005223:Fats / Q000378:metabolism*; D005243:Feces / Q000032:analysis; D005260:Female; D005990:Glycerol; D006801:Humans; D007408:Intestinal Absorption; D007410:Intestinal Diseases / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010182:Pancreatic Diseases / Q000378:metabolism; D014304:Triolein; D014316:Tritium
D002250:Carbon Radioisotopes; D005223:Fats; D014316:Tritium; D014304:Triolein; D005990:Glycerol