[Use of fluorescent probes for detecting structural reorganizations in the membranes of the hepatic endoplasmic network during the maintenance of rats on an atherogenic diet].
Prolonged feeding of rats with a full-value semi-synthetic diet or diet enriched with cholesterol provoked material changes in the lipid content (the molar ratio of cholesterol/phospholipids) in the liver endoplastic reticulum, in the functional activity (the activity of the membrane-bound glucoso-6-phosphatase) and in the physical structure of the membranes. The changes were detected by means of the fluorescent probes (pyrene, 4-dimethylaminochalcone, 1-anilinonaphthalene-8-sulfonate). All the changes were more pronounced in liver microsomes of rats fed with the atherogenic diet. Fluorescence of the probes in microsomes and in model lipid membranes (liposomes) containing varying amount of cholesterol was compared to find that in vivo changes in the physical properties of microsomal membranes do not completely reduce to the effects consequent on membrane enriching with cholesterol.
D000222:Adaptation, Physiological; D000818:Animals; D004036:Diet, Atherogenic; D004721:Endoplasmic Reticulum / Q000648:ultrastructure*; D007425:Intracellular Membranes / Q000648:ultrastructure*; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D008563:Membrane Lipids / Q000032:analysis; D008856:Microscopy, Fluorescence; D051381:Rats
D008563:Membrane Lipids
Ispol'zovanie fliuorestsentnykh zondov dlia vyiavleniia strukturnykh perestroek v membranakh éndoplazmaticheskoĭ seti pecheni pri soderzhanii krys na aterogennoĭ diete.