Cell membrane phospholipids and their constitutent fatty acids in dividing and nondividing cells of Micrococcus lysodeikticus.
Changes occurring in the cell membrane of nondividing cells of Micrococcus lysodeikticus disIIp+ grown in the presence of the mucopeptide synthesis inhibitor D-cycloserine include (a) an increae in the relative amount of diphosphatidylglycerol with a concomitant decrease in the relative content of phosphatidylglycerol, (b) a small increase in the relative palmitic acid content of phosphatidylinositol, and (c) leakage of membrane components into the growth medium. Growth of the organism in the presence of both D-cycloserine and D-alanine (which prevents the effects of D-cycloserine on cell division and mucopeptide synthesis) prevents the above changes in the cell membrane, demonstrating that secondary damage to the cell membrane can occur as a rsult of inhibition in mucopeptide synthesis. Growth of the organism in the presence of D-cycloserine and pantoyl lactone prevents the leakage of membrne components and cell division inhibition. Possible relationships of these changes to cell division are discussed.
D000409:Alanine / Q000494:pharmacology; D002308:Cardiolipins / Q000032:analysis; D002455:Cell Division; D003523:Cycloserine / Q000494:pharmacology; D005227:Fatty Acids / Q000032:analysis*; D008563:Membrane Lipids / Q000032:analysis*; D008837:Micrococcus / Q000032:analysis* / Q000254:growth & development; D010169:Palmitic Acids / Q000032:analysis; D010715:Phosphatidylglycerols / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*
D002308:Cardiolipins; D005227:Fatty Acids; D008563:Membrane Lipids; D010169:Palmitic Acids; D010715:Phosphatidylglycerols; D010743:Phospholipids; D003523:Cycloserine; D000409:Alanine