Potassium-induced relaxation of the rat anococcygeus muscle.
The nature of K-induced relaxations of the isolated rat anococcygeus muscle was studied in Tyrode's solution containing a variety of smooth muscle stimulants. When the tone of the preparation was raised by ACh (1 X 10(-6) M), the inhibitory responses to excess K (20-50 MM) were almost completely prevented by tetrodotoxin (1 X 10(-6) M) but not affected by ouabain (2 X 10(-4) M). When the preparation was contracted by barium (5 X 10(-3) M), tetraethylammonium (3 X 10(-2) M) or procaine (8 X 10(-3) M), relaxations by excess K (10-60 mM) were not affected by tetrodotoxin but completely inhibited by ouabain. It is concluded that two types of K-induced relaxations can occur depending on the smooth muscle stimulant used for elevating the tone of the preparation: the one is due to an activation of the electrogenic Na pump mediated by Na+, K+ ATPase of the smooth muscle membrane, the other is due to a stimulation of the inhibitory nerves by K.
D000818:Animals; D001464:Barium / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009126:Muscle Relaxation / Q000187:drug effects*; D009130:Muscle, Smooth / Q000187:drug effects*; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology; D011343:Procaine / Q000494:pharmacology; D051381:Rats; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology
D013757:Tetraethylammonium Compounds; D001464:Barium; D013779:Tetrodotoxin; D011343:Procaine; D010042:Ouabain; D011189:Potassium Chloride; D011188:Potassium