The effects of lymphocyte blastogenesis medium and phytohemagglutinin on the activity of PGE2 9-ketoreductase.
Components of the culture medium employed in phytohemagglutinin (PHA)-induced lymphocyte blastogenesis were tested individually to determine their effects on the conversion of PGE2 into PGF2 alpha by the enzyme, PGE2 9-ketoreductase. PHA at concentrations up to 10 microgram/ml completely inhibited the activity of PGE2 9-ketoreductase. Heat inactivated fetal bovine serum (FBS) stimulated a greater than two-fold increase in the conversion of PGE2 into PGF2 alpha. Although the mechanism of lymphocyte activation by the lectin, PHA, remains obscure, our data suggests that in vitro exposure of lymphocytes to the tissue culture medium as well as mitogens could result in intracellular alterations in prostaglandin metabolism.
D000818:Animals; D001769:Blood; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D005333:Fetus; D006168:Guinea Pigs; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism*; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000502:physiology*
D003470:Culture Media; D037102:Lectins; D006911:Hydroxyprostaglandin Dehydrogenases