Phosphorylation state of mitochondria and cytosol of 3T3 mouse cells.
Preconfluent quiescent 3T3 mouse cells, stimulated into growth with 10% serum, showed little change in their content of ATP per unit protein, or in phosphorylation state (ATP/ADP ratio) which remained near 3.0 during the initial 24 hours. In quiescent, completely confluent cells, the ATP/ADP ratio was reduced to 1.3, but increased to the normal value after 20 hours stimulation with 10% serum. These changes occurred in the cell cytoplasm; in the mitochondria the ATP/ADP ratio remained near 0.5 in both inhibited and growing cells.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D002455:Cell Division; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D003470:Culture Media; D003600:Cytosol / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D010766:Phosphorylation
D000227:Adenine Nucleotides; D003470:Culture Media; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D004247:DNA