title: On the influence of topically applied drugs on cell kinetics in the sebaceous gland. Investigations with the example of selenium disulfide in the Syrian hamster ear.
abstract: 60 male Syrian hamsters were treated on one ear with 2.5% selenium disulfide in a surfactant base and on the other ear with the same surfactant base. After treatment 15 animals were investigated by 3H-thymidine autoradiography, 15 animals by the colcemid method and 15 animals by the in vivo double labelling technique with 3H- and 14C-thymidine. In 15 animals the porportion of labeled cells was determined which still had contact with the basal lamina in the sebaceous gland 6 days after 3H-thymidine injection. Besides an increase in sebaceous gland size and in cell proliferation, a shortening of the S phase and a reduction of the labeled sebaceous gland cells which still have contact with the basal lamina of the sebaceous gland 6 days after 3H - thymidine injection were determined.
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D006224:Cricetinae; D008297:Male; D008647:Mesocricetus; D012627:Sebaceous Glands / Q000166:cytology / Q000187:drug effects*; D012643:Selenium / Q000494:pharmacology*; D018036:Selenium Compounds; D013936:Thymidine
chemical_list: D018036:Selenium Compounds; D012643:Selenium; D013936:Thymidine; C025698:selenium disulfide