5-Hydroxytryptamine-mediated inhibition of protein synthesis in rabbit reticulocyte lysate cell-free system.
Addition of 5-hydroxytryptamine (5-HT) to initiation dependent rabbit reticulocyte lysate cell-free system caused marked reductions in endogenous mRNA- and poly(U)-directed protein synthesis. Comparison of the effects on protein synthesis of 5-HT with known inhibitors of initiation (aurintricarboxylic acid and polyinosinic acid) has revealed a similar action for the compounds, suggesting that the amine may exert its inhibitory effect on the initiation of protein synthesis.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001312:Aurintricarboxylic Acid / Q000494:pharmacology; D002474:Cell-Free System; D011069:Poly I / Q000494:pharmacology; D011131:Polyribonucleotides / Q000494:pharmacology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000494:pharmacology; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors; D014364:Tryptophan / Q000494:pharmacology
D000596:Amino Acids; D011131:Polyribonucleotides; D012333:RNA, Messenger; D011069:Poly I; D012701:Serotonin; D001312:Aurintricarboxylic Acid; D014364:Tryptophan; D000255:Adenosine Triphosphate