Micro determination of gentamicin in serum by high-performance liquid chromatography with ultraviolet detection.
A procedure for the high-performance liquid chromatographic determination of gentamicin in serum is described using pre-column derivatisation and UV detection. The serum proteins are precipitated with acetonitrile and the gentamicin components in the supernatant are derivatized with 1-fluoro-2,4-dinitrobenzene. The reaction products are chromatographed on a microparticulate C18 reversed-phase column and detected at 365 nm. Sample volumes of 50 microliters are sufficient for the determination of gentamicin concentrations in, and well below, the therapeutic range.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004139:Dinitrofluorobenzene; D005839:Gentamicins / Q000097:blood*; D006207:Half-Life; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008832:Microchemistry / Q000379:methods; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013997:Time Factors
D005839:Gentamicins; D004139:Dinitrofluorobenzene