Diagnosis of hairy-cell leukaemia by tartrate-resistant acid phosphatase activity in paraffin-embedded tissue sections.
Tartrate-resistant acid phosphatase activity may be demonstrated in paraffin-embedded liver and spleen specimens as well as in decalcified bone marrow biopsies after fixation in a mixed glutaraldehyde-formaldehyde-calcium acetate solution. This technique may routinely be applied to haematologically relevant material and aids in the differential diagnosis of hairy-cell leukaemia.
D000135:Acid Phosphatase / Q000378:metabolism*; D001853:Bone Marrow / Q000201:enzymology; D003937:Diagnosis, Differential; D006651:Histocytochemistry; D006801:Humans; D007943:Leukemia, Hairy Cell / Q000175:diagnosis* / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D013154:Spleen / Q000201:enzymology; D013644:Tartrates
D013644:Tartrates; D000135:Acid Phosphatase