Effect of acriflavine on the hexokinase isozyme pattern of a yeast, Hansenula jadinii.
Dried cells of a yeast, Hansenula jadinii, that had been cultured aerobically with acriflavine, contained three hexokinase isozymes and metabolized glucose at 0.6 M to produce ATP to phosphorylate nucleotides in the presence of a high concentration of phosphate. Dried cells cultured aerobically without acriflavine contained two hexokinase isozymes and could not metabolize glucose under the same conditions. Two of the isozymes of the yeast cultured with acriflavine were similar to isozymes of the yeast cultured without acriflavine. However, the third isozyme was resistant to a high phosphate concentration and caused regeneration of ATP through glycolysis and phosphorylation of nucleotides.
D000166:Acridines / Q000494:pharmacology*; D000167:Acriflavine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D001203:Ascomycota / Q000187:drug effects*; D003566:Cytidine Diphosphate Choline / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D006593:Hexokinase / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects; D010766:Phosphorylation; D010843:Pichia / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure
D000166:Acridines; D007527:Isoenzymes; D000167:Acriflavine; D003566:Cytidine Diphosphate Choline; D000255:Adenosine Triphosphate; D006593:Hexokinase; D005947:Glucose