Measurement of choline concentration and transport in human erythrocytes by 1H NMR: comparison of normal blood and that from lithium-treated psychiatric patients.
Choline is known to accumulate in the erythrocytes of manic depressive psychotics who are treated with high doses of lithium. High field 1H spin-echo nuclear magnetic resonance was used to study human erythrocytes and measure the intracellular concentration of choline. Only 0.5 ml of cells are required and sample handing is minimal. The rate of uptake of choline was measured using a paramagnetic ferric ion complex to depress the resonance of the extracellular choline while observing the growth of the intracellular peak. The general applicability of these new observations and methods to the study and treatment regimes in manic depressive psychosis and schizo-affective disorders is discussed.
D001692:Biological Transport / Q000187:drug effects; D001714:Bipolar Disorder / Q000097:blood* / Q000188:drug therapy; D002794:Choline / Q000097:blood*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D008094:Lithium / Q000627:therapeutic use*; D009682:Magnetic Resonance Spectroscopy
D008094:Lithium; D002794:Choline