Metal analysis by energy dispersive x-ray fluorescence of bovine brain tubulin and microtubule-associated proteins prepared by phosphocellulose chromatography.
It has been shown by trace metal analysis that tubulin isolated from bovine brain does not contain strongly bound transition metal ions. The traces of zinc and iron found in the fraction of microtubule-associated proteins might originate from previously reported phosphatase activity (Larsson, H., Wallin, M. and Edström, A. (1979) J. Neurochem. 32, 155--161).
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002852:Chromatography, Ion Exchange; D002857:Chromium / Q000032:analysis; D003035:Cobalt / Q000032:analysis; D003300:Copper / Q000032:analysis; D007501:Iron / Q000032:analysis; D007854:Lead / Q000032:analysis; D008345:Manganese / Q000032:analysis; D008870:Microtubules / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009532:Nickel / Q000032:analysis; D013052:Spectrometry, X-Ray Emission / Q000379:methods; D014131:Trace Elements / Q000032:analysis*; D014404:Tubulin / Q000032:analysis; D015032:Zinc / Q000032:analysis
D009419:Nerve Tissue Proteins; D014131:Trace Elements; D014404:Tubulin; D002857:Chromium; D007854:Lead; D003035:Cobalt; D008345:Manganese; D003300:Copper; D009532:Nickel; D007501:Iron; D015032:Zinc