Two-dimensional analysis of autophosphorylated chick brain synaptic subfractions.
Chick brain synaptosomes were autophosphorylated upon incubation with [32PO4] and glucose, exposed to depolarizing ionic conditions, subsequently lysed, and respective synaptic subfractions were isolated. Analysis of these subfractions by two-dimensional electrophoresis revealed complex and unique phosphopeptide patterns. The phosphorylation levels of several soluble and membrane polypeptides appeared to change upon exposure to the depolarizing conditions.
D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002645:Chickens / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D010455:Peptides / Q000032:analysis; D010710:Phosphates / Q000378:metabolism; D010748:Phosphopeptides / Q000032:analysis*; D010750:Phosphoproteins / Q000032:analysis*; D013574:Synaptosomes / Q000032:analysis* / Q000378:metabolism
D010455:Peptides; D010710:Phosphates; D010748:Phosphopeptides; D010750:Phosphoproteins