A novel method for the determination of electrical potentials across cellular membranes. I. Theoretical considerations and mathematical approach.
A new method for the determination of electrical potentials across cellular membranes has been developed. In order to determine the membrane potential, cells were incubated in buffer solutions with increasing concentrations of KCl. Parallel experiments were performed with buffer solutions which additonally contained valinomycin. After sedimentation of the cells, the membrane potential was calculated from data which were obtained by simply measuring the wet mass, the dry mass and the potassium content of cell pellets by atomic absorption spectroscopy.
D002468:Cell Physiological Phenomena; D008433:Mathematics; D008564:Membrane Potentials; D008566:Membranes / Q000502:physiology*; D008722:Methods; D008954:Models, Biological; D011188:Potassium / Q000378:metabolism; D011189:Potassium Chloride; D014634:Valinomycin
D014634:Valinomycin; D011189:Potassium Chloride; D011188:Potassium