The ultrastructure of collagenous fibers in bone marrow long-term cultures.
The bone marrow of resected bones was studied by electron microscopy before and during cultivation on the 9th, 13th and 15th day of explantation. The absence of collagenous fibers in the inoculum and its appearance in individual sites in 9-day cultures simultaneously with growth of fibroblasts, in numerous sites in 13-day cultures and in almost all sites in 15-day cultures point to the appearance and growth of collagenous fibers after a certain cultivation time. This is also confirmed by the appearance, in the latest cultures, of collagenous fibers with transverse striation, which indicates that they mature during cultivation. The results obtained may serve as evidence of growth of collagen-producing cells (true fibroblasts) in the above-mentioned cultures.
D000293:Adolescent; D001853:Bone Marrow / Q000648:ultrastructure*; D002648:Child; D003094:Collagen / Q000032:analysis*; D003238:Connective Tissue / Q000648:ultrastructure*; D046508:Culture Techniques; D005347:Fibroblasts / Q000648:ultrastructure; D006801:Humans; D013997:Time Factors
D003094:Collagen