[Localization of the extracellular lipase in Candida paralipolytica yeasts].
The localization of lipase in the cells of Candida paralipolytica was studied for the first time by the techniques of electron cytochemistry and immunocytochemistry. Lipase is formed at the outer surface of the cytoplasmic membrane and often is confined to its invaginations. The reaction products were detected in individual parts of the periplasmic space, in the cell wall, and in its exofibrillar components. Apparently, lipase is formed on the ribosomal-membrane complex. The above pattern is observed only in the cells with a high lipase activity, but not in the control. The two techniques gave similar results. A series of electronograms are presented.
D000818:Animals; D002175:Candida / Q000201:enzymology*; D004789:Enzyme Activation; D005089:Exocytosis; D006651:Histocytochemistry; D007114:Immunization; D007120:Immunochemistry; D008049:Lipase / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D008854:Microscopy, Electron; D011817:Rabbits
D008049:Lipase
Lokalizatsiia vnekletochnoĭ lipazy u drozhzheĭ Candida paralipolytica.