Lipid content of human platelets quantitated by thin-layer chromatography in combination with flame ionization detection.
The lipid contents of human platelets from twenty-one healthy adults were analysed using thin-layer chromatography in combination with flame ionization detection. The weight per cent of neutral lipids in human platelets was 14.6%, which consisted mainly of free cholesterol, that of phosphatidylethanolamine 24.9%, phosphatidylserine plus phosphatidylinositol 6.8%, phosphatidylcholine 35.2% and sphingomyelin 18.6%. Free cholesterol in 10(8) platelets was estimated as 7 micrograms and phospholipids as 46 micrograms from calibration standards. The reproducibility was satisfactory and the procedure could be performed quickly and simply.
D000328:Adult; D001792:Blood Platelets / Q000032:analysis*; D002308:Cardiolipins / Q000097:blood; D002855:Chromatography, Thin Layer / Q000379:methods; D005260:Female; D005410:Flame Ionization / Q000379:methods; D005732:Gangliosides / Q000097:blood; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D010743:Phospholipids / Q000097:blood
D002308:Cardiolipins; D005732:Gangliosides; D008055:Lipids; D010743:Phospholipids