Simultaneous quantitation of chlormethiazole and two of its metabolites in blood and plasma by gas--liquid chromatography.
A simple and rapid gas chromatographic method for the quantitation of chlormethiazole and two of its pharmacologically active metabolites, 5-acetyl-4-methylthiazole and 5-(1-hydroxyethyl)-4-methylthiazole, in plasma and blood is described. The total analysis time is less than 20 min for a single sample. The method requires 50--500 microliter of plasma or 1 ml of blood. The compounds are detected with a nitrogen--phosphorus detector. An internal standard technique is used for the quantitation. Calibration data are linear over the range 32--2376 ng of chlormethiazole and a similar range of the metabolites in plasma. The method may be used for pharmacokinetic studies.
D000328:Adult; D002719:Chlormethiazole / Q000031:analogs & derivatives* / Q000097:blood* / Q000378:metabolism; D002849:Chromatography, Gas / Q000379:methods; D005260:Female; D006801:Humans; D007700:Kinetics; D010949:Plasma / Q000032:analysis; D011247:Pregnancy; D011263:Pregnancy Trimester, Third
D002719:Chlormethiazole; C025062:5-acetyl-4-methylthiazole; C025061:5-(1-hydroxyethyl)-4-methylthiazole