Gas chromatographic determination of the fatty acid pattern of red cell membrane plasmalogens in healthy children.
A gas chromatographic technique for the determination of the fatty acid pattern of the phosphatidylcholine (PC) and phosphatidyl ethanolamine (PE) plasmalogen and non-plasmalogen fractions in human red cell membranes has been developed. The coefficient of variation lies between 5 and 10%. The technique has been applied to blood samples of healthy Belgian school children (8--10 year old). PE contains 47% plasmalogen and 53% non-plasmalogen form, whereas PC occurs only in its non-plasmalogen form. The fatty acid pattern of the various fractions and subfractions has been determined.
D002648:Child; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D005227:Fatty Acids / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D010713:Phosphatidylcholines / Q000097:blood / Q000302:isolation & purification; D010714:Phosphatidylethanolamines / Q000097:blood / Q000302:isolation & purification; D010955:Plasmalogens / Q000097:blood* / Q000302:isolation & purification; D012737:Sex Factors
D005227:Fatty Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010955:Plasmalogens