Cell surface turnover and shedding of fucosyl-glycoprotein in L6 cells.
The disappearance of electrophoretically identifiable 3H-fucosylated glycoproteins from the cell surface of L6 cells is random and follows monophasic kinetics with a half-life of 9 hours. Approximately 47% and 53% of that loss is due to shedding and to degradation, respectively. Shedding also occurs with monophasic kinetics. Most of the electrophoretically identifiable-shed fucosyl-glycoproteins are released with homogeneous rates but do not represent a random sampling of the surface.
D000818:Animals; D005110:Extracellular Space / Q000378:metabolism; D005643:Fucose; D006023:Glycoproteins / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D007739:L Cells / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D011485:Protein Binding
D006023:Glycoproteins; D008565:Membrane Proteins; D005643:Fucose