Inhibition of uterine estrogen receptor transformation by sodium molybdate.
The effects of sodium molybdate on the transformation of mouse uterine cytoplasmic estradiol . receptor complex has been studied. If molybdate is added to cytosol containing the nontransformed estradiol . receptor complex, it blocks the receptor transformation which otherwise occurs during incubation at 28 degrees C. The inhibition by molybdate is apparent both in sucrose gradient centrifugation analyses of estradiol . receptor complexes and [3H]estradiol dissociation kinetic studies. A molybdate-sensitive change of the nontransformed estradiol . receptor complex can also be inferred from behavior of the estradiol . receptor complex on low ionic strength sucrose gradients.
D000818:Animals; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D003600:Cytosol / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D005260:Female; D051379:Mice; D008982:Molybdenum / Q000494:pharmacology*; D011960:Receptors, Estrogen / Q000187:drug effects* / Q000378:metabolism; D013696:Temperature; D014599:Uterus / Q000378:metabolism*
D011960:Receptors, Estrogen; D004958:Estradiol; D008982:Molybdenum