Enhanced anti-inflammatory effect and reduced immunogenicity of bovine liver superoxide dismutase by conjugation with homologous albumin.
Bovine liver superoxide dismutase (SOD) can be cross-linked to albumin to form soluble conjugates with up to 70% retention of original enzyme activity. These conjugates have markedly prolonged plasma half-times of enzymatic activity (15 h) compared to native SOD (6 min). The conjugates have potent anti-inflammatory effects in vivo and reduced immunogenicity and antigenicity compared to native SOD. These conjugates provide new possibilities for the study of inflammation and its treatment.
D000418:Albumins / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000893:Anti-Inflammatory Agents; D000917:Antibody Formation; D002351:Carrageenan / Q000037:antagonists & inhibitors; D002417:Cattle; D002850:Chromatography, Gel; D004305:Dose-Response Relationship, Drug; D004356:Drug Storage; D004487:Edema / Q000188:drug therapy; D007106:Immune Sera / Q000276:immunology; D007457:Iodine Radioisotopes; D008099:Liver / Q000201:enzymology*; D008657:Metabolic Clearance Rate; D008970:Molecular Weight; D011817:Rabbits; D051381:Rats; D013482:Superoxide Dismutase / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D014018:Tissue Distribution
D000418:Albumins; D000893:Anti-Inflammatory Agents; D007106:Immune Sera; D007457:Iodine Radioisotopes; D002351:Carrageenan; D013482:Superoxide Dismutase