Reversed-phase, ion-pair liquid chromatography of quaternary ammonium compounds: determination of pyridostigmine, neostigmine and edrophionium in biological fluids.
A reversed-phase, ion-pair liquid chromatographic method for the quantitative determination of quaternary acetylcholinesterase inhibitors is described. The method uses an ion-pair extraction to isolate the drugs from biological material prior to liquid chromatographic separation and online UV detection at 214 nm. Quantiation down to 5 ng/ml and within-day precison with coefficient of variation (C.V.) of 1.5% (n = 10, x = 100 ng/ml) for neostigmine, C.V., 1.7% (n = 10, x = 80 ng/ml) for pyridostigmine and C.V., 1.5% (n = 10, x = 100 ng/ml) for edrophonium have been achieved. The assay was designed for pharmacokinetic studies of these drugs in anesthetized patients.
D002853:Chromatography, Liquid / Q000379:methods*; D004491:Edrophonium / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D009388:Neostigmine / Q000032:analysis* / Q000097:blood / Q000652:urine; D011729:Pyridostigmine Bromide / Q000032:analysis* / Q000097:blood / Q000652:urine
D009388:Neostigmine; D004491:Edrophonium; D011729:Pyridostigmine Bromide