Detection of an enzyme activity which cleaves m7 guanine from m7 GMP in an extract of embryonic chick lens cells.
m7Guanine was cleaved from m7GMP by cytoplasmic enzyme activity in an extract prepared from embryonic chick lens cells. The appearance of m7-Guanine was proportional to the time and concentration of extract. m7-Guanine inhibited the reaction but neither guanine nor ribose 5-phosphate did. m7Guanine was not released from m7Guanosine. m7Guanine may be derived from m7GpppG mRNA cap by two enzymatic reactions with m7CMP as a product-substrate intermediate.
D000818:Animals; D002642:Chick Embryo; D004590:Electrophoresis, Paper; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D007700:Kinetics; D007908:Lens, Crystalline / Q000201:enzymology*; D012314:RNA Cap Analogs / Q000378:metabolism*; D012315:RNA Caps / Q000378:metabolism*
D012314:RNA Cap Analogs; D012315:RNA Caps; C011925:7-methylguanosine-5'-monophosphate; D006147:Guanine; C008450:7-methylguanine