title: Rat lens epithelial cells in vitro. II. Changes of protein patterns during aging and transformation.
abstract: Serially subcultured rat lens epithelial cells grow in different stages, which can be classified according to morphology, chromosome numbers and population kinetics. A lens-specific gamma-crystallin appears in the diploid stage, when elongated cell types are observed. One of the beta-crystallin bands (pH 5.7) disappears during aging in higher passage numbers of the diploid stage B. A weak band in the beta-crystallin region (pH 6.4), which is present in all stages, becomes very intensive in aneuploid cells of stage D, which exhibit a fibroblast-like morphology.
mesh_terms: D000818:Animals; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003459:Crystallins / Q000032:analysis*; D004847:Epithelial Cells; D005347:Fibroblasts / Q000166:cytology; D007908:Lens, Crystalline / Q000032:analysis / Q000166:cytology*; D011003:Ploidies; D051381:Rats
chemical_list: D003459:Crystallins