[Directed modification of the early region of T7 bacteriophage DNA by alkylation in the R-loop formed by a modified transcript].
For addressed modification of T7 phage DNA a polyalkylating derivative of T7 phage early transcript was used. The derivative was obtained by attaching polyfunctional alkylating agent N,N,N,'-tri(beta-chloroethyl)-N'-(p-formylphenyl) propylene diamine-1,3 to the transcript, their molecules being covalently bound by alkylating 4--5% of the RNA adenine and guanine residues. The polyalkylating RNA was used to form R-loops in the complementary site of T7 DNA to alkylate selectively the T7 phage early DNA. Alkylation of DNA by the modified transcript in the region of the R-loop led to the covalent binding of the transcript to the complementary site of DNA. The correct localization of this R-loop was confirmed by electron microscopy. The application of the results for the addressed mutagenesis and inactivation of selected genes is discussed.
D000478:Alkylation; D004279:DNA, Viral; D003959:Diamines; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D013604:T-Phages / Q000235:genetics*; D014158:Transcription, Genetic
D004279:DNA, Viral; D003959:Diamines
Napravlennaia modifikatsiia ranneĭ oblasti DNK bakteriofaga T7 putem alkilirovaniia v R-petle, obrazovannoĭ modifitsirovannym transkriptom.