Isolation and preliminary characterization of herpes Channel Catfish virus DNA.
The DNA of Channel Catfish virus (CCV) was selectively extracted from infected cells with a 5% solution of sodium deoxycholate, deproteinized using sodium sarcosinate and pronase, and purified by phenol extraction followed by equilibrium density gradient centrifugation in a cesium chloride solution. CCV DNA displays a buoyant density of 1.715 g/cm3 in such a solution, as would be expected from a duplex DNA containing 56.1% of guanine plus cytosine. As estimated from both its sedimentation coefficient and length in the electron microscope, CCV DNA is a linear duplex molecule of approximately 85 x 10(6) daltons.
D000818:Animals; D002478:Cells, Cultured; D002500:Centrifugation, Isopycnic; D004279:DNA, Viral / Q000302:isolation & purification*; D005399:Fishes / Q000382:microbiology; D006564:Herpesviridae / Q000032:analysis* / Q000254:growth & development; D008854:Microscopy, Electron; D008970:Molecular Weight
D004279:DNA, Viral