Effect of concanavaline A on intracellular K+ and Na+ concentration and K+ transport of human lymphocytes.
Incubation of human lymphocytes with ConA causes an increase in [Na+]i and a decrease in [K+]i. This effect is not due to the experimental washing procedure, but is due to the ConA-induced increase in permeability which is not fully compensated by the increase in active transport. The ConA-induced increase in 42K+ uptake consists of an increase in leak flux which is independent of [Na+]o, and of an increase in pump flux which is dependent on [Na+]o. The increase in leak flux may be caused by increased membrane fluidity. The increase in pump flux may be produced by the increased [Na+]i and by a stimulation of Na+, K+ATPase.
D001665:Binding Sites; D001692:Biological Transport; D003208:Concanavalin A / Q000494:pharmacology*; D005110:Extracellular Space; D006801:Humans; D007444:Inulin / Q000494:pharmacology; D008214:Lymphocytes / Q000378:metabolism*; D009994:Osmolar Concentration; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
D003208:Concanavalin A; D010042:Ouabain; D007444:Inulin; D012964:Sodium; D011188:Potassium