title: Synthesis of 21-diazo-9 alpha-fluoro-16 alpha-methyl-21-deoxy-[1,2(n)-3H]prednisolone.
abstract: The preparation of 21-diazo-9 alpha-fluoro-16 alpha-methyl-21-deoxy-[1,2(n)-3H]prednisolone is described. [1,2(n)-3H]Dexamethasone 26 Ci/mmol, was oxidized with cupric acetate/air to give the 21-aldehyde. Treatment of the aldehyde with hydroxylamine gave the corresponding 21-oxime which was reacted with chloroamine to give a 25--35% yield of 21-diazo-9 alpha-fluoro-16 alpha-methyl-21-[1,2(n)-3H]deoxyprednisolone with a specific activity of 25--26 Ci/mmol.
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D003600:Cytosol / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008775:Methylprednisolone / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D051381:Rats
chemical_list: D003907:Dexamethasone; D008775:Methylprednisolone