Monoclonal rat anti-mouse brain antibody detects Abelson murine leukemia virus target cells in mouse bone marrow.
We report the characterization of a monoclonal antibody which detects a surface antigen expressed by the bone marrow target cell of A-MuLV. Treatment of bone marrow cells with this antibody and complement results in greater than loss 95% loss of the A-MuLV-derived in vitro transformed foci. The surface antigen detected by this antibody is also expressed on A-MuLV-transformed lymphoid cell lines, thymocytes, and some peripheral lymphocytes. This antigen is not expressed, however, by the pluripotent hematopoietic stem cell defined by the spleen colony-forming assay. We present evidence that the antigen detected is neither a virally encoded product, nor exclusively associated with the BALB/c genome.
D000011:Abelson murine leukemia virus; D000818:Animals; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D001853:Bone Marrow / Q000276:immunology*; D001854:Bone Marrow Cells; D001921:Brain / Q000276:immunology*; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D014764:Viral Proteins / Q000276:immunology
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D014764:Viral Proteins