Basement membrane collagens. Cyanogen bromide peptides of the D chain from porcine kidney.
An alpha chain size collagenous component, designated as the D chain, was isolated from the pepsin digest of porcine kidney cortices. Reduction of the D chain with 2-mercaptoethanol and carboxymethylation with iodoacetic acid resulted in the formation of two smaller components of 75 000 and 15 000 molecular weights. The 75 000 and 15 000 molecular weight components were separated by molecular sieve chromatography on a column of agarose A-5M. The 75 000 molecular weight component was cleaved with CNBr in 70% formic acid. The resulting CNBr peptides were isolated by a combination of ion exchange and molecular sieve chromatography and were characterized for amino acid contents and molecular weights. A total of seven CNBr peptides were obtained, which together accounted for the amino acid content of the intact 75 000 molecular weight component.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000032:analysis*; D003094:Collagen; D003488:Cyanogen Bromide; D007461:Iodoacetates; D007672:Kidney Cortex / Q000032:analysis*; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding; D013552:Swine
D000596:Amino Acids; D007461:Iodoacetates; D010446:Peptide Fragments; D003094:Collagen; D003488:Cyanogen Bromide