title: Detection by alkaline elution of rat liver DNA damage induced by simultaneous subacute administration of nitrite and aminopyrine.
abstract: DNA fragmentation induced in the livers of rats by oral treatment with NaNO2 and aminopyrine was evaluated by the alkaline elution technique. Whereas simultaneous administration of the two compounds in a single dose produced only a minimal increase of the DNA elution rate, their intake with drinking water for 20 successive days caused DNA fragmentation comparable to that observed after a single ip injection of 10-20 mg/kg N-nitrosodimethylamine. Either NaNO2 or aminopyrine alone induced borderline DNA damaging effects, if any, in both rats receiving a single dose and those treated for 20 successive days.
mesh_terms: D000632:Aminopyrine / Q000633:toxicity*; D000818:Animals; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism*; D004128:Dimethylnitrosamine / Q000633:toxicity; D004347:Drug Interactions; D008099:Liver / Q000032:analysis / Q000378:metabolism*; D008297:Male; D009573:Nitrites / Q000633:toxicity*; D051381:Rats
chemical_list: D009573:Nitrites; D000632:Aminopyrine; D004247:DNA; D004128:Dimethylnitrosamine