Three-step purification of glucocorticoid receptors from rat liver.
The 90 000-Mr glucocorticoid receptor was purified to homogeneity according to sodium dodecylsulfate/polyacrylamide gel electrophoresis. An affinity column containing either dexamethasone-17 beta-carboxylic acid or dexamethasone-21-methanesulfonate bound to the matrix with the help of a disulfide bond is used in this study. Using this affinity matrix, in a single step, 8700-fold purification of glucocorticoid receptor from rat liver cytosol could be achieved. Following the method of activation and DNA-cellulose chromatography the 90 000-Mr receptor subunit was purified to homogeneity.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D003600:Cytosol / Q000032:analysis; D004247:DNA; D003907:Dexamethasone / Q000031:analogs & derivatives; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis* / Q000648:ultrastructure; D008297:Male; D008970:Molecular Weight; D051381:Rats; D011965:Receptors, Glucocorticoid / Q000302:isolation & purification*; D011987:Receptors, Steroid / Q000302:isolation & purification*
D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003907:Dexamethasone; D004247:DNA