Ketone body transport in renal brush border membrane vesicles.
Ketone body uptake by renal brush border vesicles has been investigated. Ketone bodies enter into the brush border vesicles by a carrier-mediated process. The uptake is dependent on an Na+ gradient ([Na+]outside>[Na+]inside) and is electroneutral. The uptake is transport into an osmotically active space and not a binding artifact as indicated by the effect of increasing the medium osmolarity. A pH gradient (alkaline inside) also stimulates the ketone body uptake. Acetoacetate and 3-hydroxybutyrate share the same carrier as demonstrated by the accelerated exchange diffusion and mutual inhibitory effects.
D000090:Acetoacetates / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism*; D004058:Diffusion; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D007657:Ketone Bodies / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000378:metabolism*; D009861:Onium Compounds / Q000378:metabolism; D009943:Organophosphorus Compounds; D051381:Rats
D000090:Acetoacetates; D000596:Amino Acids; D006885:Hydroxybutyrates; D007476:Ionophores; D007657:Ketone Bodies; D009861:Onium Compounds; D009943:Organophosphorus Compounds; C013289:tetraphenylphosphonium