Cooperative effects between lymphokine-induced and antibody-dependent macrophage-mediated cytotoxicity in C57NL/10 and C3H/HeJ mice.
Cooperative effects are described between 2 different mechanisms of macrophage-mediated cytotoxicity, the lymphokine-induced and the antibody-dependent cytotoxicity:Macrophages are activated to nonspecific cytotoxicity by a 24 h incubation with lymphokines. Young macrophages cultured from the bone-marrow or obtained from induced peritoneal exudates, but not the resident peritoneal macrophages, have the capacity to kill antibody-coated tumor cells. To obtain a cooperative effect between the 2 mechanisms both the lymphokine and the antibody have been titrated to a dilution, at which the macrophages did not exert any cytotoxicity. At such subthreshold dosages, however, a very good synergistic effect between the 2 mechanisms was observed. This synergism was obtained with bone-marrow macrophages and with thioglycollate-induced peritoneal macrophages. Macrophages of C3H/HeJ mice, which have been reported to be a low responder strain for lymphokine activation, showed synergistic effects with similar lymphokine concentrations as macrophages from the high responder strain C57BL/10. Thus, in this synergistic situation the low responsiveness of C3H/HeJ mice is completely compensated. Since during an immune reaction in most cases lymphokine as well as antibody are produced, this synergistic reaction will be of particular relevance for in vivo-situations.
D000818:Animals; D000918:Antibody Specificity; D000920:Antibody-Dependent Cell Cytotoxicity; D001854:Bone Marrow Cells; D002450:Cell Communication; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology; D008222:Lymphokines / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL
D008070:Lipopolysaccharides; D008222:Lymphokines