Binding of non-mammalian immunoglobulins to staphylococcal protein A.
Immunoglobulins M of the chicken (about 3% of serum IgM), clawed toad (12%) and carp (13%) bind to the protein A-Sepharose. A monomeric immunoglobulin with four domain heavy chains from the chicken serum is also bound (30% of bound immunoglobulins). The protein A-Sepharose can be utilized for isolation of IgM of the clawed toad and carp directly from the serum.
D000818:Animals; D002347:Carps; D002645:Chickens; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D007075:Immunoglobulin M / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism*; D008970:Molecular Weight; D013205:Staphylococcal Protein A / Q000378:metabolism*; D014982:Xenopus laevis
D007075:Immunoglobulin M; D007136:Immunoglobulins; D013205:Staphylococcal Protein A