Occurrence of bile alcohols in the bile of a patient with cholestasis.
Considerable amounts (6.0 mumol/ml of bile) of bile alcohols were found in the bile of a patient with cholestasis due to gallstones in a common bile duct. Acid hydrolysis of the bile salts followed by chromatographic separation yielded three bile alcohols, which were identified as 26,27-dinor-5 beta-cholestane-3 alpha,7 alpha,12 alpha,24,25-pentol, 24-methyl-26,-27-dinor-5 beta-cholestane-3 alpha,7 alpha, 24-tetrol, and 3 alpha,7 alpha, 12 alpha-trihydroxy-26,27-dinor-5 beta-cholestan-24-one by comparison with synthetic reference standards.
D000368:Aged; D001646:Bile / Q000032:analysis*; D002777:Cholestanols / Q000032:analysis*; D002779:Cholestasis / Q000378:metabolism*; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005966:Glucuronidase; D006801:Humans; D008297:Male; D013058:Mass Spectrometry
D002777:Cholestanols; D005966:Glucuronidase