title: Localization of adenylate cyclase in the neurointermediate lobe of the rat pituitary. Ultrastructural cytochemistry.
abstract: The ultrastructural localization of adenylate cyclase was accomplished cytochemically in the neurointermediate lobe of Sprague-Dawley rats. The main concentration of the reaction product was found on the plasmalemma of neurosecretory nerve fibers, their terminals and plasma membranes of pituicytes. Positive reaction for adenylate cyclase was found less regularly in endothelial cells, pericapillary spaces and processes of the basal lamin. The septum between the pars nervosa and the pars intermedia showed heavy deposits of the reaction product, especially around the neurosecretory nerve fibers but also around other types of nerve fibers. Reaction for adenylate cyclase was not seen in the cells of the pars intermedia. When the substrate (ATP) was omitted, no reaction product was found. These findings support the suggestion of an involvement of cyclic AMP in the release mechanism of neurohypophysial of an involvement of cyclic AMP in the release mechanism of neurohypophysial hormones from the neurosecretory nerve terminals, and possibly also their transfer into blood vessels and perivascular channels.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D004727:Endothelium / Q000201:enzymology; D005110:Extracellular Space / Q000201:enzymology; D007030:Hypothalamo-Hypophyseal System / Q000201:enzymology; D008297:Male; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000201:enzymology; D010902:Pituitary Gland / Q000201:enzymology*; D010904:Pituitary Gland, Posterior / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats
chemical_list: D000262:Adenylyl Cyclases