Human cutaneous lieshmania in a mouse macrophage line: propagation and isolation of intracellular parasites.
A mouse macrophage line, J774G8, supports continuous and prolific intracellular growth of Leishmania mexicana amazonensis, the etiological agent of a South American cutaneous leishmaniasis. The intracellular parasites from these infected cultures can be isolated with high recovery rate and purity by simple Percoll gradient centrifugation.
D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006801:Humans; D007891:Leishmania / Q000254:growth & development; D007896:Leishmaniasis / Q000469:parasitology* / Q000473:pathology; D008264:Macrophages / Q000469:parasitology*; D051379:Mice