Cell-free synthesis of glial fibrillary acidic protein.
Glial fibrillary acidic (GFA) protein has been synthesized in an RNA-dependent cell-free system derived from rabbit reticulocytes. The cell-free synthesized product appears to have the same size as GFA protein isolated from bovine spinal cord, thus showing that GFA protein does not undergo detectable proteolytic processing.
D000818:Animals; D001369:Axons / Q000378:metabolism; D002417:Cattle; D002474:Cell-Free System; D005904:Glial Fibrillary Acidic Protein; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D010446:Peptide Fragments / Q000032:analysis; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D013116:Spinal Cord / Q000378:metabolism*
D005904:Glial Fibrillary Acidic Protein; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger