A rapid method of isolation and fractionation of proteoglycans using a vertical tube rotor for isopycnic centrifugation.
A comparison was made between a vertical tube rotor and a fixed angle rotor for isopycnic centrifugation of proteoglycans. In the vertical tube rotor, isopycnic gradient was achieved much faster than in the conventional fixed angle rotor. The use of a vertical tube rotor for isopycnic centrifugation shortens the time considerably for the isolation of proteoglycans from various tissues.
D000818:Animals; D002356:Cartilage / Q000032:analysis; D002417:Cattle; D002500:Centrifugation, Isopycnic / Q000295:instrumentation / Q000379:methods; D005591:Chemical Fractionation; D008168:Lung / Q000032:analysis; D011509:Proteoglycans / Q000302:isolation & purification*; D013997:Time Factors
D011509:Proteoglycans