Studies on megakaryocytes: isolation from rat and guinea pig and incorporation of 5-hydroxytryptamine.
A new, two step method is described for the isolation of megakaryocytes from bone marrow. By means of centrifugation in a PercollR density gradient megakaryocytes can be enriched by factor 68 or 24 starting from rat or guinea pig bone marrow, respectively. The purity of the megakaryocytes can be improved ca. 4 fold if gradient centrifugation is followed by separation in an Elutriator rotor.-The isolated megakaryocytes take up exogenous 5-hydroxytryptamine with a time and concentration dependent transport mechanism, K11=30 micrometer, K12=1.5 micrometer. As shown by electron microscopy the number and density of storage granules within the megakaryocytes is enhanced after incubation with 5-hydroxytryptamine. This finding suggests that 5-hydroxytryptamine is incorporated into the organelles.
D000818:Animals; D001854:Bone Marrow Cells; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D006168:Guinea Pigs; D006651:Histocytochemistry; D008533:Megakaryocytes / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D013045:Species Specificity
D012701:Serotonin