title: A new chromosome region possibly derived from double minutes in an in vitro transformed epithelial cell line.
abstract: Double minute chromosomes (DMs) are reported for the first time in in vitro transformed mouse epithelial cells. In one cell line, CSG 122/17, DMs persisted through numerous passages. A subpopulation appeared in this line at passage 23, in which the DMs had disappeared but were replaced by a finely banded chromosome region possibly associated with the distal end of chromosome 5. In a second cell line, CSG 120/7, there was no evidence of DMs in the earliest frozen stock available. However, a finely banded region similar to that found in CSG 122/17 was present and was again associated with chromosome 5, in this case with the proximal end. The possible evolution of these new chromosome regions from DMs is discussed.
mesh_terms: D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure; D051379:Mice; D012469:Salivary Glands