[Isolation and characteristics of the DNA of donor and systemic lupus erythematosus patient blood plasma].
DNA from blood plasma of healthy donors and patients with systemic lupus erythematosus (SLE) was isolated by various techniques. Adsorption chromatography of hydroxyapatite with subsequent deproteinization has shown that DNA of blood plasma from both healthy donors and patients with SLE is associated with serum protein and is low-molecular. This combination (DNA--protein) is resistant to the action of nuclease but susceptible to pronase. DNA that circulates in blood of SLE patients in conjunction with immunoglobulins is high-molecular as compared to DNA associated with other serum proteins.
D000327:Adsorption; D004247:DNA / Q000097:blood* / Q000302:isolation & purification; D003956:Dialysis; D006801:Humans; D006882:Hydroxyapatites; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D008722:Methods; D008970:Molecular Weight; D011402:Pronase; D013050:Spectrometry, Fluorescence
D006882:Hydroxyapatites; D004247:DNA; D011402:Pronase
Vydelenie i kharakteristika DNK plazmy krovi donorov i bol'nykh sistemnoĭ krasnoĭ volchanki.