The effect of fluoride on the state of aggregation of adenylate cyclase in rat liver plasma membranes.
Irradiation inactivation was used to monitor changes in the state of adenylate cyclase in rat liver plasma membranes in the presence of F-.F- caused a decrease in the target size from 328000 to 237000 at 0 degrees C and from 329000 to 219000 at 30 degrees C. Adenylate cyclase was activated by F- at both 0 degrees C and 30 degrees C. The effect of F- was biphasic, activating up to a concentration of 10mM and inhibiting at higher concentrations. If adenylate cyclase weas maximally activated with glucagon and p[NH]ppG ([beta gamma-imido]GTP) all concentrations of F- were inhibitory. The implications of the results with respect to the mechanism of activation of adenylate cyclase are discussed.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005459:Fluorides / Q000494:pharmacology*; D005934:Glucagon / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D046911:Macromolecular Substances; D051381:Rats; D012969:Sodium Fluoride / Q000494:pharmacology; D013696:Temperature
D046911:Macromolecular Substances; D006165:Guanylyl Imidodiphosphate; D012969:Sodium Fluoride; D005934:Glucagon; D000262:Adenylyl Cyclases; D005459:Fluorides