Separation of alphavirus nucleocapsids from envelope fragments.
Methods for the preparation of alphavirus nucleocapsids and envelope fragments for biochemical, electron microscopical and serological investigations are described. Difficulties which have been encountered previously in attempts to isolate viral nucleocapsids for morphological studies, namely disruption of the fragile particles, have been overcome by treatment with formaldehyde.
D001103:Arboviruses / Q000032:analysis / Q000648:ultrastructure*; D002213:Capsid / Q000302:isolation & purification*; D008854:Microscopy, Electron; D008970:Molecular Weight; D012672:Semliki forest virus / Q000032:analysis; D012845:Sindbis Virus / Q000032:analysis; D013045:Species Specificity; D014764:Viral Proteins / Q000302:isolation & purification*
D014764:Viral Proteins