title: Detection of an enzyme activity which cleaves m7 guanine from m7 GMP in an extract of embryonic chick lens cells.
abstract: m7Guanine was cleaved from m7GMP by cytoplasmic enzyme activity in an extract prepared from embryonic chick lens cells. The appearance of m7-Guanine was proportional to the time and concentration of extract. m7-Guanine inhibited the reaction but neither guanine nor ribose 5-phosphate did. m7Guanine was not released from m7Guanosine. m7Guanine may be derived from m7GpppG mRNA cap by two enzymatic reactions with m7CMP as a product-substrate intermediate.
mesh_terms: D000818:Animals; D002642:Chick Embryo; D004590:Electrophoresis, Paper; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D007700:Kinetics; D007908:Lens, Crystalline / Q000201:enzymology*; D012314:RNA Cap Analogs / Q000378:metabolism*; D012315:RNA Caps / Q000378:metabolism*
chemical_list: D012314:RNA Cap Analogs; D012315:RNA Caps; C011925:7-methylguanosine-5'-monophosphate; D006147:Guanine; C008450:7-methylguanine