Effect of tunicamycin and cycloheximide on the secretion of acid hydrolases from I-cell cultured fibroblasts.
I-cell cultures fibroblasts secrete excessive amounts of N-acetyl-beta-D-hexosaminidase and alpha-L-fucosidase into the culture media as compared with normal fibroblasts. Addition of tunicamycin or cyd [14C]leucine (40--50%) into trichloroacetic acid-precipitable material decreased the secretion of these I-cell hydrolases to normal values within 24 h, but had no effect on the secretion of acid hydrolases from normal fibroblasts. These results indicate that I-cell cultured fibroblasts secrete at least two types of acid hydrolases: one is tunicamycin- and cycloheximide-sensitive and constitutes the greater proportion of the secreted hydrolases, and a smaller proportion is insensitive to tunicamycin and cycloheximide, similar t9 the acid hydrolases secreted by normal cultured fibroblasts.
D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology; D005944:Glucosamine / Q000031:analogs & derivatives*; D006596:Hexosaminidases / Q000096:biosynthesis*; D006801:Humans; D007930:Leucine / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D009081:Mucolipidoses / Q000201:enzymology*; D014415:Tunicamycin / Q000494:pharmacology*; D005644:alpha-L-Fucosidase / Q000096:biosynthesis*
D014415:Tunicamycin; D003513:Cycloheximide; D006596:Hexosaminidases; D005644:alpha-L-Fucosidase; D007930:Leucine; D005944:Glucosamine; D008358:Mannose