Effect of mercuric chloride on microsomal enzyme system in mouse liver.
The effect of mercuric chloride (2 mg/kg) on microsomal enzymes in mouse liver was investigated in phenobarbital- and 3-methylcholanthrene-induced hepatic microsomes. The metal caused an increase in total microsomal protein in noninduced controls, whereas no significant changes were observed in induced microsomes. Cytochrome P-450 was decreased in each case, which suggests that the metal may cause enzyme degradation. p-Nitroanisole O-demethylase was increased fivefold with both inducers. In addition, mercuric chloride increased the O-demethylase activity in relation to microsomal protein. The effect of the metal on ethylmorphine N-demethylase activity was slightly inhibitory.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D005037:Ethylmorphine-N-Demethylase / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008628:Mercury / Q000494:pharmacology*; D008748:Methylcholanthrene / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D009575:Nitroanisole O-Demethylase / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism
D011506:Proteins; D008748:Methylcholanthrene; D003577:Cytochrome P-450 Enzyme System; D009575:Nitroanisole O-Demethylase; D005037:Ethylmorphine-N-Demethylase; D008628:Mercury; D010634:Phenobarbital