Erythrocyte transketolase, a new semi-automated method.
This paper describes a method to determine transketolase activity in erythrocytes, with or without addition of thiamine-pyrophosphate, by quantitating the formation of glucose-6-phosphate forming during the incubation of hemolysed blood with ribose-5-phosphate. Glucose-6-phosphate is determined enzymatically, after deproteinisation of the sample, using a centrifugal fast analyser. The proposed method gave a correlation of r = 0.89 with an accepted colorimetric method. Reference values for blood are in the range 32--101 U/l.
D001322:Autoanalysis / Q000379:methods*; D004912:Erythrocytes / Q000201:enzymology*; D005958:Glucosephosphates / Q000378:metabolism; D006801:Humans; D010428:Pentosephosphates; D014174:Transketolase / Q000378:metabolism*
D005958:Glucosephosphates; D010428:Pentosephosphates; D014174:Transketolase