Thymidine phosphorylase. Substrate specificity for 5-substituted 2'-deoxyuridines.
A continuous spectrophotometric assay for thymidine phosphorylase has been developed and used to analyze the substrate properties of a number of 5-substituted 2'-deoxyuridines. A QSAR was found which relates the catalytic efficiency for various substrates to a single variable, the inductive field constant F.
D000818:Animals; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000378:metabolism; D006736:Horses; D066298:In Vitro Techniques; D008954:Models, Biological; D010430:Pentosyltransferases / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D013939:Thymidine Phosphorylase / Q000378:metabolism*
D010430:Pentosyltransferases; D013939:Thymidine Phosphorylase; D003857:Deoxyuridine