Molarity and ionic strength of focused carrier ampholytes in isoelectric focusing.
Focused 1% carrier ampholytes in the pH range 3.5--10 have a molarity of 9--10 mM, as determined by ismolarity measurements of fractions focused in free water. From electrophoretic and isoelectric focusing data of red blood cells, it has been demonstrated that the corresponding ionic strength is 0.5 mg-ions/l. Also, theoretical considerations and conductivity measurements point to a value in the range of 0.5--1.0 mg-ions/l. The following equations for ionic strength (I) calculations have been derived: 1 = 1/20 C amph + CH in the pH range 2.5--7 and I = 1/20 Camph + COH in the pH range 7--11.
D000665:Ampholyte Mixtures; D002021:Buffers; D004912:Erythrocytes / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*
D000665:Ampholyte Mixtures; D002021:Buffers