Flash-induced kinetic infrared spectroscopy applied to biochemical systems.
A flash photolysis apparatus with monitoring infrared beam is described allowing measurements of relative transmission changes of 10(-3) in times of a few milliseconds. The investigation of the photodissociation of CO-myoglobin confirms the results obtained by static infrared difference spectroscopy. The application of our method to the rhodopsin/Meta II transition reveals signals which can tentatively be ascribed to the disappearance of the C = C-band of the protonated N-retinylidene Schiff base in rhodopsin. The developed method will be compared with other existing methods of kinetic vibronic spectroscopy such as kinetic resonance Raman spectroscopy and kinetic Fourier infrared spectroscopy.
D000818:Animals; D002248:Carbon Monoxide / Q000032:analysis; D007700:Kinetics; D009211:Myoglobin / Q000032:analysis*; D010782:Photolysis; D012168:Retinal Pigments / Q000032:analysis*; D012243:Rhodopsin / Q000032:analysis*; D013055:Spectrophotometry, Infrared / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors
D009211:Myoglobin; D012168:Retinal Pigments; D002248:Carbon Monoxide; D012243:Rhodopsin