Enzymic and electrophoretic characterization of the hexachlorobenzene-induced cytochrome P-450 in the liver of rat.
1. Hexachlorobenzene induces the cytochtome P-450 system in rat liver microsomes. The catalytic activity of the enzyme towards various substrates (dealkylation of 7-ethoxycoumarin, hydroxylation of biphenyl and NADPH-dependent reduction of cytochrome c) corresponds to that obtained in animals treated with a mixture of phenobarbital and benzpyrene. 2. Electrophoretic separation of the partly purified hexachlorobenzene-induced cytochrome P-450 in sodium dodecyl sulphate polyacrylamide gels exhibits a protein pattern similar to that found in microsomes from rats treated with phenobarbital plus benzpyrene.
D000818:Animals; D001580:Benzopyrenes / Q000494:pharmacology; D002722:Chlorobenzenes / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D006581:Hexachlorobenzene / Q000494:pharmacology*; D006868:Hydrolysis; D008099:Liver / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats
D001580:Benzopyrenes; D002722:Chlorobenzenes; D006581:Hexachlorobenzene; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital