Identification of a macromolecular inhibitor of in vitro lymphocyte proliferation in human seminal plasma as bound spermine.
The excluded fraction from Sephadex G-25 separation of human seminal plasma has been found to suppress lymphocyte transformation and DNA synthesis [7]. We have shown that most of its in vitro activity is due to contamination by spermine which binds tenaciously to, and co-elutes with the protein.
D002850:Chromatography, Gel; D004247:DNA / Q000096:biosynthesis; D003864:Depression, Chemical; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D012661:Semen / Q000032:analysis*; D013096:Spermine / Q000032:analysis* / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism
D013096:Spermine; D004247:DNA; D013936:Thymidine