Bovine J blood-group activity in the lipids of erythrocytes of different age.
J-positive cattle erythrocytes were separated according to age by ultracentrifugation in a discontinuous density gradient of Ficoll-400 (Pharmacia) in isotonic buffer solution. A decrease in phospholipid and in cholesterol content with increasing age was detected. The J activity was found to increase markedly with increasing cell age. This fact supports the view that the in vivo transfer of the J determinant from plasma to erythrocytes is a rather slow process. It is suggested that the increasing J activity of older cells is probably due to a decrease of erythrocyte membrane bound phospholipids which inhibit the transfer of the J determinant to the red cells.
D000367:Age Factors; D000818:Animals; D001789:Blood Group Antigens; D002417:Cattle / Q000097:blood*; D002784:Cholesterol / Q000097:blood; D004912:Erythrocytes / Q000032:analysis*; D008055:Lipids / Q000097:blood*; D010743:Phospholipids / Q000097:blood
D001789:Blood Group Antigens; D008055:Lipids; D010743:Phospholipids; D002784:Cholesterol