High-performance liquid chromatographic analysis of sulfinpyrazone and its metabolites in biological fluids.
A rapid, senstivie, and specific high-performance liquid chromatographic method is described for the quantitative analysis of sulfinpyrazone and its sulfone and p-hydroxy metabolites in plasma and urine. The method uses two different procedures for sample preparation: (1) a rapid and convenient procedure using a single extraction with 1-chlorobutane and subsequent back-extraction into sodium hydroxide solution for the analysis of sulfinpyrazone and its sulfone metabolite, and (2) a more time consuming procedure using triple extraction with ethylene dichloride, a buffer wash, and back extraction into the base for the additional analysis of the p-hydroxy metabolite. The lower limit of sensitivity for fulfinpyrazone is 50 ng/ml. Concentrations of sulfinpyrazone between 0.05 and 0.1 and 50 micrograms/ml were measured with an average coefficient of variation of 3.9%, ranging from 1.5 to 6.1%.
D002073:Butanes; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005025:Ethylene Dichlorides; D006801:Humans; D008297:Male; D013442:Sulfinpyrazone / Q000031:analogs & derivatives* / Q000097:blood* / Q000378:metabolism / Q000652:urine; D013450:Sulfones / Q000097:blood / Q000652:urine
D002073:Butanes; D005025:Ethylene Dichlorides; D013450:Sulfones; C025186:4-hydroxysulfinpyrazone; D013442:Sulfinpyrazone; C006854:butyl chloride