Reversible binding of the chemoattractant folic acid to cells of Dictyostelium discoideum.
The chemoattractant folic acid binds reversibly to receptors at the surface of Dictyostelium cells. In undifferentiated cells (t0.5) 6 X 10(4) binding sites per cell with a K0.5 value of 1.5 X 10(-7) M were determined. The number of folic acid receptors per cell decreases slightly during cell development. In differentiated cells (t10) 3.3 X 10(4) binding sites per cell were estimated. The folic acid binding sites appear to be specific for folic acid and its derivatives. 2-Deamino-2-hydroxyfolic acid, 4-amino-folic acid (aminopterin), and 4-amino-10-methylfolic acid (amethopterin) compete for the folic-acid-binding sites. Nor do adenosine 3',5'-phosphate and guanosine 3',5'-phosphate affect binding of folic acid. The folic acid receptors appear to be distinct from the catalytic sites of the membrane-bound folic acid deaminase.
D001667:Binding, Competitive; D002630:Chemotactic Factors; D004023:Dictyostelium / Q000254:growth & development / Q000378:metabolism*; D005492:Folic Acid / Q000031:analogs & derivatives / Q000378:metabolism*; D007700:Kinetics; D011955:Receptors, Drug / Q000378:metabolism*
D002630:Chemotactic Factors; D011955:Receptors, Drug; D005492:Folic Acid