Isolation of a non-adhesive mutant of Vibrio cholerae and chromosomal localization of the gene controlling mannose-sensitive adherence.
A mutant (CD11) of Vibrio cholerae was isolated after exposure of a wild-type pathogenic strain (KB207) to N-methyl-N'-nitro-N-nitrosoguanidine. Although the mutant was motile and chemotactic, it adhered poorly to rabbit intestinal mucosa. Pretreatment of vibrios with 10 mg D-mannose ml-1 and of intestinal mucosa with 100 mmol sodium metaperiodate inhibited adherence of KB207 but not of CD11. The gene (ams) controlling mannose-sensitive adherence was mapped on the bacterial chromosome closely linked to the pur locus.
D000268:Adhesiveness; D000818:Animals; D002874:Chromosome Mapping; D005796:Genes; D006386:Hemagglutination Tests; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000382:microbiology; D008358:Mannose / Q000494:pharmacology*; D009154:Mutation; D011817:Rabbits; D011995:Recombination, Genetic; D014734:Vibrio cholerae / Q000187:drug effects / Q000235:genetics*
D008358:Mannose