[Ultrastructural changes in plague microbes of vaccinal strain EB and alveolar macrophages of guinea pigs during their interaction in vitro].
Aerosol immunization with plague vaccine EB was found to stimulate an increase in the number of free alveolar macrophages and to enhance their ingesting activity which correlated with an increase in specific antibody titers. Immunological transformation was accompanied by ultrastructural changes in the cytoplasm and the nuclei of alveolar macrophages. The phagocytosis of microbes occurred by their adsorption onto the microphage surface, invagination and enclosing into the endocytic bubble having the function of the digestive vacuole. The growth of the number of macrophages and an increase in their ingesting activity, progressive changes in the cytoplasmic structures may be used as indicators of immunological transformation resulting from the administration of plague vaccine by the inhalation method.
D000336:Aerosols; D000818:Animals; D004705:Endocytosis; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008264:Macrophages / Q000382:microbiology* / Q000648:ultrastructure; D008854:Microscopy, Electron; D010587:Phagocytosis; D010930:Plague / Q000517:prevention & control*; D010931:Plague Vaccine / Q000008:administration & dosage; D011650:Pulmonary Alveoli / Q000166:cytology*; D014611:Vaccination; D015010:Yersinia pestis / Q000648:ultrastructure*
D000336:Aerosols; D010931:Plague Vaccine
Ul' trastrukturnye izmeneniia v chumnykh mikrobakh vaktsinnogo shtamma EB i al'veoliarnykh makrofagakh morskikh svinok pri vzaimodeĭstvii ikh in vitro.