Changes in synthesis of types-I and -III collagen during matrix-induced endochondral bone differentiation in rat.
The changes in rates of hydroxyproline formation and biosynthesis of types-I and -III collagen during bone matrix-induced sequential differentiation of cartilage, bone and bone marrow in rat were investigated. Biosynthesis of types-I and -III collagen at different stages of this sequence was studied by labelling in vivo and in vitro with [2,3-3H]proline. Pepsin-solubilized collagens were separated by sodium dodecyl sulphate/polyacrylamide-slab-gel electrophoresis. The results revealed that maximal amounts of type-III collagen were synthesized on day 3 during mesenchymal-cell proliferation. Thereafter, there was a gradual decline in type-III collagen synthesis. On days 9--20 during bone formation predominantly type-I collagen was synthesized. Similar results were obtained by the use of labelling techniques both in vivo and in vitro.
D000818:Animals; D001846:Bone Development; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D001857:Bone Matrix; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism; D002356:Cartilage / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation; D003094:Collagen / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D006909:Hydroxyproline / Q000096:biosynthesis; D008297:Male; D011392:Proline / Q000378:metabolism; D051381:Rats
D003094:Collagen; D011392:Proline; D006909:Hydroxyproline