Analysis of the multigene family coding the developmentally regulated carbohydrate-binding protein discoidin-I in D. discoideum.
Using recombinant DNA technology, we have isolated chimeric plasmids carrying genomic or cDNA sequences complementary to mRNA encoding the developmentally regulated carbohydrate-binding protein Discoidin-I. The protein is encoded by a 4-5 member multigene family. Mapping of the genomic sequences by DNA blot hybridization suggests that several of the genes are closely linked. RNA excess hybridization kinetics indicate that Discoidin-I mRNA is found in less than one copy per gene per cell during vegetative growth, and increases 500-1000 fold during the first 6 hr of development. The Discoidin-I mRNA concentration decreases during later stages of development. mRNA complementary to the genes shows two bands of -1.0-1.1 kb on acrylamide gels, indicating heterogeneity either in the genes or in mRNA processing.
D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004023:Dictyostelium / Q000235:genetics*; D000070821:Discoidins; D005656:Fungal Proteins / Q000235:genetics*; D005796:Genes; D008040:Genetic Linkage; D037102:Lectins; D015800:Protozoan Proteins; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D002352:Carrier Proteins; D004274:DNA, Recombinant; D000070821:Discoidins; D005656:Fungal Proteins; D037102:Lectins; D015800:Protozoan Proteins; D012331:RNA, Fungal; D012333:RNA, Messenger