Gangliosides and neutral glycolipids of human adrenal medulla.
Glycolipids were isolated from human adrenal medulla by DEAE-Sephadex A-25 and Iatrobeads column chromatography. The lipid-bound sialic acid was about 234 microgram/g fresh tissue. The glanglioside fraction contained two major gangliosides which accounted for 93% of the total lipid-bound sialic acid. They were identified as GM3, N-acetylneuraminylgalactosylglucosylceramide and GD3, N-acetylneuraminyl N-acetylneuraminylgalactosylglucosylceramide on the basis of cochromatography with authentic standards, sugar composition analysis, and neuraminidase digestion. GM3, N-acetylneuraminylgalactosylglucosylceramide and GD3, N-acetylneuraminyl N-acetylneuraminylgalactosylglucosylceramide occurred in a ratio of approximately 3 : 2, and the ratio seemed to be rather constant irrelevant of age and sex differences. The neutral glycolipid fraction consisted of GL1a, glucosylceramide (18%), GL1b, galactosylceramide (23%), GL2a, lactosylceramide (27%), GL3, digalactosylglucosylceramide (20%), and GL4, globoside (12%). The major fatty acids of all these glycolipids were 16 : 0, 18 : 0, 22 : 0, 24 : 0 and 24 :1.
D000313:Adrenal Medulla / Q000032:analysis*; D000328:Adult; D000368:Aged; D002554:Cerebrosides / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005260:Female; D005679:G(M3) Ganglioside / Q000032:analysis; D005699:Galactosylceramides / Q000032:analysis; D005732:Gangliosides / Q000032:analysis*; D005915:Globosides / Q000032:analysis; D005963:Glucosylceramides / Q000032:analysis; D006017:Glycolipids / Q000032:analysis*; D006801:Humans; D007790:Lactosylceramides / Q000032:analysis; D008297:Male; D008875:Middle Aged
D002554:Cerebrosides; D005227:Fatty Acids; D005679:G(M3) Ganglioside; D005699:Galactosylceramides; D005732:Gangliosides; D005915:Globosides; D005963:Glucosylceramides; D006017:Glycolipids; D007790:Lactosylceramides; C026225:digalactosylglucosylceramide; C026226:ganglioside, GD3