Identification of platelet activating factor isolated from rabbit basophils as acetyl glyceryl ether phosphorylcholine.
Platelet activating factor obtained from antigen-stimulated rabbit buffy coat leukocytes containing 10% IgE-sensitized basophils was shown by gas-liquid chromatographic and mass spectrometric analysis of products after acetolysis and by base and acid hydrolysis to be acetyl-alkylglyceryl phosphorylcholine. The alkyl side chains were composed of octadecyl (greater than 90%) and hexadecyl (less than 10%) residues.
D000818:Animals; D001491:Basophils / Q000032:analysis*; D002849:Chromatography, Gas; D007073:Immunoglobulin E; D008244:Lysophosphatidylcholines / Q000097:blood* / Q000302:isolation & purification; D013058:Mass Spectrometry; D010972:Platelet Activating Factor; D011817:Rabbits
D008244:Lysophosphatidylcholines; D010972:Platelet Activating Factor; D007073:Immunoglobulin E