Freeze-fracture autoradiography of the red blood cell plasma membrane.
A procedure for electron microscope autoradiography of red blood cell ghosts or their outer membrane leaflets is described: sheep erythrocytes, attached to positively charged mica, were either lysed and air-dried, or freeze-fractured "by hand" and freeze-dried. This was followed by shadowing and carbon replication; coating with photographic emulsion; exposure in darkness at 277 K; developing and fixation; carbon coating; floating off the mica and mounting on grids. Erythrocytes iodinated with 125I using the lactoperoxidase reaction were used as a test object. The efficiency of the procedure was found to be between 3.4% and 5.6%.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000648:ultrastructure*; D004912:Erythrocytes / Q000648:ultrastructure*; D005614:Freeze Fracturing; D007457:Iodine Radioisotopes; D008854:Microscopy, Electron; D012756:Sheep
D007457:Iodine Radioisotopes