[Covalent C-terminal hydrophilisation of cyanogen bromide peptides with arginine in the sequenator (author's transl)].
Cyanogen bromide peptides are modified with arginine (free base) at the C-terminal homoserin lactone. This modification makes the peptides more hydrophilic. In that way the degradation is possible up to homoserine and the C-terminal arginine. The phenylthiohydantoin derivatives of homoserine and arginine can be detected in good yields by thin-layer or high performance liquid chromatography. The automatic Edman degradation is investigated under various conditions.
D000595:Amino Acid Sequence; D001120:Arginine / Q000378:metabolism*; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D003488:Cyanogen Bromide / Q000378:metabolism*; D010443:Peptide Chain Termination, Translational; D010669:Phenylthiohydantoin / Q000378:metabolism
D010669:Phenylthiohydantoin; D001120:Arginine; D003488:Cyanogen Bromide
Kovalente, C-terminale Hydrophilisierung von Bromcyanpeptiden mit Arginin im Sequenator.