Evidence for the absence of I-E/C antigen expression on the cell surface in mice of the H-2b or H-2s haplotypes.
Mouse Ia antigens encoded by I-E/C subregions of the H-2b and H-2s haplotypes have not previously been detected with alloantisera. We have therefore examined the reactivity of xenoantisera, which could potentially detect nonpolymorphic determinants of these antigens, by using a sequential immunoprecipitation analysis of internally labeled glycoproteins from spleen lymphocytes of H-2b and H-2s haplotypes. The first xenoanti-serum, BN rat anti-B10 (H-2b) mouse, contained anti-Ia reactivity but precipitated only I-A antigens from both H-2b preparations and from preparations of another haplotype (H-2a) that is known to express I-E/C alloantigens. The second xenoantiserum, goat anti-Ia, was raised against soluble Ia antigens of H-2d haplotype. This serum lysed lymphocytes of all H-2 haplotypes tested and precipitated both I-A and I-E/C antigens from H-2d antigen preparations. However, when b or s haplotype antigens were examined, this antiserum precipitated only I-A antigens. These observations favor the hypothesis that I-E/C antigens are not expressed in these haplotypes. This hypothesis offers a possible explanation for the broad interspecies cross-reactions of anti-I-E/C alloantisera raised in either H-2b or H-2s haplotypes and may have implications for studies of two-gene control of immune responses.
D000818:Animals; D000918:Antibody Specificity; D000954:Antigens, Surface; D011232:Chemical Precipitation; D005260:Female; D006041:Goats; D006183:H-2 Antigens; D006238:Haploidy; D006824:Hybridization, Genetic; D007106:Immune Sera / Q000494:pharmacology; D007519:Isoantigens; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D051381:Rats; D011914:Rats, Inbred BN
D000954:Antigens, Surface; D006183:H-2 Antigens; D007106:Immune Sera; D007519:Isoantigens