Catalysis of adenovirus DNA synthesis in vitro by DNA polymerase gamma.
DNA replication complexes were purified from adenovirus type 5 (Ad5)-infected HeLa cells. DNA synthesis by these complexes in vitro was extremely sensitive to the inhibitors dideoxythymidine triphosphate, N-ethyl maleimide and p-hydroxymercuribenzoate. The bound DNA polymerase was released from the complexes by limited digestion with micrococcal nuclease. This released polymerase preferred poly(rA):(dT)12-18 as template over activated calf thymus DNA. These results are compatible with the major polymerase in the replication complex being of the gamma class.
D000256:Adenoviridae / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D054306:Dideoxynucleotides; D005033:Ethylmaleimide / Q000494:pharmacology; D006902:Hydroxymercuribenzoates / Q000494:pharmacology; D012093:Replicon; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000494:pharmacology
D004279:DNA, Viral; D054306:Dideoxynucleotides; D006902:Hydroxymercuribenzoates; D013942:Thymine Nucleotides; D004259:DNA-Directed DNA Polymerase; C017216:2',3'-dideoxythymidine triphosphate; D005033:Ethylmaleimide