Catabolism of prostaglandin F2 alpha in rat ovary : differences between ovarian and uterine tissues.
The catabolism in vitro of prostaglandin F2 alpha (PGF2 alpha) in rat ovarian homogenate was studied comparing with uterine homogenate. Two kinds of metabolite were recognized by incubation of PGF2 alpha with ovarian or uterine homogenate; 13, 14-dihydro-15-keto-PGF2 alpha (15KD-PGF2 alpha) and 13, 14-dihydro-PGF2 alpha (13, 14H2-PGF2 alpha) in ovarian homogenate and 15-keto-PGF2 alpha (15K-PGF2 alpha) and 15KD-PGF2 alpha in uterine homogenate. Incubation of 15KD-PGF2 alpha with ovarian homogenate resulted in the formation of 13, 14H2-PGF2 alpha but incubation with uterine homogenate did not produce 13, 14H2-PGF2 alpha. 13, 14H2-PGF2 alpha was in accord with Rf value of a compound formed by reduction of 15KD-PGF2 alpha with sodium borohydride.
D000818:Animals; D002855:Chromatography, Thin Layer; D015237:Dinoprost / Q000031:analogs & derivatives*; D005260:Female; D010053:Ovary / Q000378:metabolism*; D010084:Oxidation-Reduction; D011460:Prostaglandins F / Q000378:metabolism*; D051381:Rats; D014599:Uterus / Q000378:metabolism*
D011460:Prostaglandins F; C026527:13,14-dihydroprostaglandin F2alpha; C008228:15-keto-13,14-dihydroprostaglandin F2alpha; C026528:15-ketoprostaglandin F2alpha; D015237:Dinoprost