The use of rRNA-cellulose chromatography in the rapid isolation of homogeneous protein synthesis initiation factor eIF-2 from rat liver microsomes.
A rapid and efficient procedure is described for preparing homogeneous polypeptide chain initiation factor eIF-2 from rat liver microsomal salt wash. The method involves ammonium sulfate fractionation, affinity chromatography on rRNA-cellulose and DEAE-cellulose chromatography. The preparation is highly active in initiation complex formation.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D015852:Eukaryotic Initiation Factor-2; D046911:Macromolecular Substances; D008862:Microsomes, Liver / Q000378:metabolism*; D008970:Molecular Weight; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000302:isolation & purification* / Q000378:metabolism; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D012335:RNA, Ribosomal; D011817:Rabbits; D051381:Rats
D015852:Eukaryotic Initiation Factor-2; D046911:Macromolecular Substances; D010448:Peptide Initiation Factors; D011506:Proteins; D012335:RNA, Ribosomal