High-pressure liquid chromatographic assay for chloramphenicol, chloramphenicol-3-monosuccinate, and chloramphenicol-1-monosuccinate.
A high-pressure liquid chromatographic method for the sensitive and rapid determination of chloramphenicol, chloramphenicol-3-monosuccinate, and chloramphenicol-1-monosuccinate is presented. The procedure utilizes a reversed-phase chromatographic column with UV absorption detection. The assay is useful for monitoring patients receiving chloramphenicol, determining the pharmacokinetics of parenteral chloramphenicol sodium succinate, and certifying sterile chloramphenicol sodium succinate.
D001798:Blood Proteins / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D002701:Chloramphenicol / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D006801:Humans; D007700:Kinetics; D011485:Protein Binding
D001798:Blood Proteins; D002701:Chloramphenicol; C026016:chloramphenicol succinate