[L line cell differentiation. Histochemical and electron microscopic studies].
In a medium supplemented with 60 per cent large cattle serum, part of the cell population of L-transformed culture was seen to be differentiated into mature lipocytes. The formation of structures similar to normal fat tissue took place in the same conditions, provided a reduced quantity of the seeded cells was used. The cell differentiation was accompanied with a decrease in acid phosphatase and non-specific esterase activities. The ultrastructural study has demonstrated a suitable preservation of cytoplasmic structures and a domination of the rough cytoplasmic reticulum compared to the control cell cultures where free ribosomes and polysomes predominated.
D000135:Acid Phosphatase / Q000378:metabolism; D000273:Adipose Tissue / Q000648:ultrastructure; D002454:Cell Differentiation; D003470:Culture Media; D004789:Enzyme Activation; D004950:Esterases / Q000378:metabolism; D006651:Histocytochemistry; D007739:L Cells / Q000201:enzymology / Q000648:ultrastructure*; D008854:Microscopy, Electron; D013997:Time Factors
D003470:Culture Media; D004950:Esterases; D000135:Acid Phosphatase
[Differentsirovka kletok linii L. Gistokhimicheskoe iélektronno-mikroskopicheskoe issledovaniia.