The use of networks of dissociated rat dorsal root ganglion neurons to induce myelination by oligodencrocytes in culture.
A neuronal culture system has been developed that has the demonstrated ability to induce myelin formation by added oligodendrocytes. Networks of dissociated dorsal root ganglion neurons were prepared by suppressing non-neuronal cells (i.e. fibroblasts and Schwann cells) with a continuous 2 week exposure to 10(-5)M fluorodeoxyuridine in the culture medium. After drug withdrawal, neuroglial cells were introduced in optic nerve implants from 1-2 week-old rats. These added glial cells migrated extensively over the unensheathed neurites and central myelin was formed by 2 weeks after the implant addition.
D000818:Animals; D002454:Cell Differentiation; D005727:Ganglia, Spinal / Q000166:cytology*; D008854:Microscopy, Electron; D009185:Myelin Proteins / Q000096:biosynthesis*; D009186:Myelin Sheath / Q000166:cytology; D009415:Nerve Net / Q000166:cytology*; D009420:Nervous System / Q000166:cytology*; D009457:Neuroglia / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology*; D009900:Optic Nerve / Q000166:cytology; D051381:Rats
D009185:Myelin Proteins