Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes.
Specific initiation of transcription of cloned conalbumin and ovalbumin genes has been obtained in a cell-free system in the presence of purified calf thymus RNA polymerase B. Comparison with the adenovirus-2 early (E1A) and major late genes reveals marked variations in transcriptional efficiencies. Specific fragmentation of the conalbumin gene DNA indicates that a short DNA segment 5' to the initiation site is involved in specific transcription.
D000260:Adenoviruses, Human / Q000235:genetics*; D003207:Conalbumin / Q000235:genetics*; D004274:DNA, Recombinant; D004527:Egg Proteins / Q000235:genetics*; D005796:Genes; D005809:Genes, Regulator; D005814:Genes, Viral; D009876:Operon; D010047:Ovalbumin / Q000235:genetics*; D012319:RNA Polymerase II / Q000378:metabolism; D013045:Species Specificity; D013698:Templates, Genetic; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D004527:Egg Proteins; D003207:Conalbumin; D010047:Ovalbumin; D012319:RNA Polymerase II