title: Segregation of leech neurones by the effect of sparteine on action potential duration.
abstract: 1. Sparteine (SPT) and 3- or 4-aminopyridine, were applied to leech segmental ganglia and the electrophysiological responses of the Retzius (R) and sensory neurones responding to pressure (P), touch (T), and noxious (N) stimuli analysed. 2. SPT 0.05-0.5 mM when presented via the bath to the whole ganglion prolonged the action potentials of these neurones to characteristically different degrees; the cells were clearly segregated in the order R greater than N greater than P greater than T at 0.5 mM, regardless of exposure time. 3. The plateau of the prolonged action potentials in the R and N cells was sustained by either Ca or Sr and was blocked by Mn, in normal or Na-free Ringer. These responses were similar to those seen in the same cell types with TEA under the same condition. 4. The SPT prolongation of action potentials was favoured by alkalinization of the Ringer solution. This implies that the drug acted in its uncharged form. SPT was ineffective when applied by pressure into the somata of these four neurones. This may be because SPT was charged at the intracellular pH or because it acted at some external membrane site. 5. The aminopyridines when applied in the bath had no effect on the repolarization of these four neurones. 6. These results suggest that TEA and SPT probably act on repolarization by similar mechanisms. The parameter of membrane function principally affected is probably a K current which contributes to repolarization to different degrees in the four cells and which may be activated by Ca.
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000631:Aminopyridines / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007865:Leeches / Q000502:physiology*; D008274:Magnesium / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D013034:Sparteine / Q000494:pharmacology*; D013324:Strontium / Q000494:pharmacology
chemical_list: D000631:Aminopyridines; D013034:Sparteine; D008274:Magnesium; D002118:Calcium; D013324:Strontium