Studies of the integrity of iodinated bovine albumin after circulating in vivo.
The integrity of 125 I BSA circulating in vivo was assessed by immunoprecipitation, antibody binding, and sephadex chromatography. 125 I BSA measurements obtained by precipitation in trichloroacetic acid agreed closely with measurements obtained by antibody binding and immuno - precipitation for up to 12 days after injection or until 99.5% of the 125 I BSA was eliminated. The molecular weight of the circulating 125 I, after dissociation of immune complexes, was identical to non-labelled BSA. 99.5% of the BSA was eliminated from the circulation 10 to 15 days after injection. After this time 45% of the circulating 125 I which precipitated in trichloroacetic acid was not bound to BSA.
D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens; D001666:Binding Sites, Antibody; D002417:Cattle; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D007457:Iodine Radioisotopes; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000941:Antigens; D007457:Iodine Radioisotopes; D012710:Serum Albumin, Bovine