Simple electrophoretic technique for creatine kinase MM isozyme in neonatal Duchenne muscular disease screening using dried blood samples.
By increasing diadenosine pentaphosphate to a final concentration of 75 mumol to 100 mumol/l to inhibit excess adenylate kinase, a highly sensitive substrate in a gelatin matrix allowed the developement of a simple and cheap electrophoretic technique for creatine kinase MM isozyme in dried blood samples. The method is suitable for neonatal Duchenne muscular dystrophy screening, and it is an alternative method for a previously described bio-luminescent assay, which requires special equipment.
D000227:Adenine Nucleotides / Q000494:pharmacology; D000263:Adenylate Kinase / Q000378:metabolism; D003402:Creatine Kinase / Q000097:blood*; D004586:Electrophoresis / Q000379:methods*; D004791:Enzyme Inhibitors; D006801:Humans; D007231:Infant, Newborn; D007527:Isoenzymes / Q000097:blood*; D008297:Male; D009136:Muscular Dystrophies / Q000175:diagnosis* / Q000235:genetics
D000227:Adenine Nucleotides; D004791:Enzyme Inhibitors; D007527:Isoenzymes; D003402:Creatine Kinase; D000263:Adenylate Kinase