[Method of contrast enhancement of DNA-containing structures on ultrathin sections].
Thin epoxy sections of formaldehyde-fixed tissue underwent depurinizing hydrolysis with 5N HCl at room temperature for 1 hour, the treatment with an aldehyde blocking agent, 10% sodium bisulfite, at 60 degrees C during 90 min, and staining with 2% aqueous uranyl acetate. Introduction of bisulfite considerably enhanced the contrast of chromatin. Staining of DNA in these conditions is summed up of preferential binding of uranyl cations with phosphates of DNA and with bisulfite anions specifically bound to deoxyribose.
D000818:Animals; D004247:DNA / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D051381:Rats
D004273:DNA, Neoplasm; D004247:DNA
Metodika spetsificheskogo usileniia kontrasta DNK-soderzhashchikh struktur na ul'tratonkikh srezakh.