TLC determination of griseofulvin in plasma and 6-demethylgriseofulvin in urine.
Fluorometric TLC procedures are described for the determination of griseofulvin in human plasma and 6-demethylgriseofulvin in human urine. Griseofulvin is extracted from plasma with ether, and its metabolite, 6-demthylgriseofulvin, is extracted from urine with benzene. Both compounds are subjected to TLC on silica gel plates. The plates are scanned using the fluorescent mode of a spectrodensitometer. For griseofulvin, the quantitation limit is 20 ng/ml of plasma and the recovery is 100%; for 6-demethylgriseofulvin, the limit is 1 microgram/ml of urine and the recovery is 90%. The methods were used to determine the plasma levels of griseofulvin and the amount of 6-demethylgriseofulvin excreted in the urine of human volunteers after a single oral dose of griseofulvin.
D002855:Chromatography, Thin Layer / Q000379:methods*; D003640:Dealkylation; D005470:Fluorometry; D005966:Glucuronidase; D006118:Griseofulvin / Q000031:analogs & derivatives* / Q000097:blood* / Q000652:urine; D006801:Humans; D006868:Hydrolysis
C024017:6-demethylgriseofulvin; D006118:Griseofulvin; D005966:Glucuronidase