Effect of sex and Mycoplasma synoviae infection on chicken red blood cells used for hemagglutination-inhibition test.
A total of 10 male and 10 female 9-week-old commercial Mycoplasma-free broiler chickens was used in this test. Five males and 5 females were artificially infected by aerosol with a broth culture of Mycoplasma synoviae (MS) F10-2AS. The other 5 males and 5 females were used as noninfected controls. At 3, 9, and 12 weeks postinfection all birds were bled, and the blood was pooled into 4 groups: infected male, noninfected male, infected female, and noninfected female. Hemagglutination-inhibition (HI) tests for MS and Mycoplasma gallisepticum (Mg) were run on a selected group of known positive serums using the 4 pools as red blood cell (RBC) sources. The geometric mean HI titers of the 4 groups showed no significant differences between male and female or MS-infected and noninfected chickens as a source of RCB's for the MS or Mg HI test. These results indicate that if procedures are correct, there is no danger in using RBC's from MS-infected birds.
D000818:Animals; D002645:Chickens; D004909:Erythrocyte Indices; D005260:Female; D006385:Hemagglutination Inhibition Tests; D008297:Male; D009175:Mycoplasma Infections / Q000276:immunology / Q000662:veterinary*; D011201:Poultry Diseases / Q000276:immunology*