A rapid and simple method for measurement of total protein in very low density lipoproteins by the Lowry assay.
When total protein in very low density lipoprotein samples is measured by the method of Lowry et al. (1951. J. Biol. Chem 193:265-275), turbidity remains in the final color reaction. Addition of 0.1 ml of 2.5% (v/v) solution of Triton X-100 removes this turbidity immediately and effectively. This simple modification is faster, less cumbersome, and more economical than removing turbidity with ethyl ether or chloroform. Addition of 0.1 ml of 2.5% (w/v) sodium dodecyl sulfate to the final reaction mixture is also effective in removing turbidity and can be used as an alternative method.
D002725:Chloroform; D004986:Ether; D006801:Humans; D008079:Lipoproteins, VLDL / Q000097:blood*; D008722:Methods; D017830:Octoxynol; D011092:Polyethylene Glycols; D011506:Proteins / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D013053:Spectrophotometry
D008079:Lipoproteins, VLDL; D011506:Proteins; D004986:Ether; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D002725:Chloroform; D017830:Octoxynol