[Effect of neutral red on the cytotoxic activity of olivomycin].
Olivomycin accumulation in living cells first of all occurs in the cytoplasm or probably the cell lysosomes. The nuclear fluorescence of the cells incubated in olivomycin solution was observed in 60-90 minutes. Simultaneously with the nuclear fluorescence there was observed disappearance of the cytoplasm fluorescence. Intensive nuclear fluorescence was recorded in 15-30 minutes in 60-70% of the cells incubated in a solution of neutral red and subsequently treated with olivomycin. The amount of olivomycin bound to the nuclear DNA in dead cells in the presence of Mg++ and at pH 7.2 was so high that the cell nuclei produced yellow-green fluorescence. No binding of olivomycin to the nuclear DNA was observed in acid media. The cytotoxic activity of olivomycin after incubation with the human fibroblasts in a solution of neutral red increased 100 times. Olivomycin may be used for determination of vital activity of the cells when their lysosome function is not impaired.
D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004347:Drug Interactions; D005347:Fibroblasts / Q000187:drug effects*; D005453:Fluorescence; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects*; D009499:Neutral Red / Q000494:pharmacology*; D009848:Olivomycins / Q000494:pharmacology*; D010619:Phenazines / Q000494:pharmacology*; D013997:Time Factors
D010619:Phenazines; D009499:Neutral Red; D009848:Olivomycins
Vliianie neĭtral'nogo krasnogo na tsitotoksicheskuiu aktivnost' olivomitsina.