Cryopreservation of granulocytes for transfusion: studies on human granulocyte isolation, the effect of glycerol on lysosomes, kinetics of glycerol uptake and cryopreservation with dimethyl sulfoxide and glycerol.
D000818:Animals; D001793:Blood Preservation / Q000379:methods*; D001803:Blood Transfusion; D002463:Cell Membrane Permeability; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004912:Erythrocytes / Q000502:physiology; D005615:Freezing; D005966:Glucuronidase / Q000032:analysis; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology*; D006098:Granulocytes / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000502:physiology; D008247:Lysosomes / Q000502:physiology*; D009997:Osmotic Pressure; D010587:Phagocytosis; D011817:Rabbits
D003451:Cryoprotective Agents; D005966:Glucuronidase; D005990:Glycerol; D004121:Dimethyl Sulfoxide