A new optical technique for evaluating collective motility of ram and bull ejaculated spermatozoa.
A new, non-destructive, objective technique for measuring collective motility of highly-concentrated ram and bull semen is described. The principle is based on changes in the reflected light scattered by motile spermatozoa. These changes can be recorded as a continuous analog wave pattern (Reflectospermiogram-RSG) and are correlated to the intensity of the turbulent motility as evaluated subjectively with an ordinary light microscope. Ram spermatozoa have, after ejaculation, a typical motility pattern, i.e., high, stable activity for about 20 min, then a period during which the motility decreases at a constant rate, and finally, a period with a low but rather constant activity. The usefulness of the technique has been demonstrated in various types of experiments.
D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002417:Cattle; D004864:Equipment and Supplies; D006863:Hydrogen-Ion Concentration; D008297:Male; D012756:Sheep; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000502:physiology*; D013696:Temperature; D013997:Time Factors
D002110:Caffeine