[The role of hemagglutinin cleavage in influenza virus adsorption and penetration into the cell].
Adsorption and penetration into chick fibroblast cells of WSN virus with completely cleaved hemagglutinin precursor (virus grown in chick embryos), partially cleaved hemagglutinin precursor (virus grown in chick fibroblasts), and virus treated with trypsin added to infected cells or virions were studied. Treatment with trypsin in both instances resulted in complete cleavage of hemagglutinin precursor and in more than 100-fold increase of the infectious activity. Chick embryo-grown virus adsorbed in the same manner as the culture-grown virus not treated with trypsin, but penetrated into the cells 2--3 times more effectively. The culture-grown virus after treatment with trypsin adsorbed better but penetrated in the same way as the original culture-grown virus. Treatment of the infected cells with trypsin led to more effective penetration with unchanged adsorption. The experimental results suggest that cleavage of hemagglutinin precursor is essential both for adsorption and penetration. Depending on the method of treatment, the effectiveness of one or the other process is increased.
D000327:Adsorption; D000818:Animals; D002642:Chick Embryo; D006389:Hemagglutinins, Viral; D009975:Orthomyxoviridae / Q000187:drug effects / Q000472:pathogenicity / Q000502:physiology*; D014357:Trypsin / Q000494:pharmacology; D014776:Virus Cultivation
D006389:Hemagglutinins, Viral; D014357:Trypsin
Rol' narezaniia gemaggliutinina v adsorbtsii i proniknovenii virusa grippa v kletku.