Absence of in vitro dexamethasone-dependent lipolytic activity from highly purified growth hormone.
Partially purified human GH was subjected to chromatogrphy on DEAE-cellulose to yield a preparation that lacked in vitro lipolytic activity in the rat epididymal fat pad even in the presence of dexamethasone. Release of both glycerol and FFA was examined after a 4-h incubation and was found to be unaffected by concentrations of GH as high as 0.1 mg/ml incubation medium. The lipolytic activity of the original partially purified GH was detected in an acidic fraction from the DEAE-cellulose chromatography. Similar results were obtained with bovine GH. Adipose tissue from rats fed Purina rat chow was more responsive to the lipolytic factor than tissue from rats fed a high sucrose, fat-free diet.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D004041:Dietary Fats / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D013006:Growth Hormone / Q000494:pharmacology*; D008053:Lipid Mobilization / Q000187:drug effects*; D008297:Male; D051381:Rats
D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D003907:Dexamethasone; D013006:Growth Hormone; D005990:Glycerol