title: Localization of g2 light chain in the link between the heads and tail of cardiac myosin.
abstract: Cardiac myosin liberated 1.99 mol of acetate per mol after acid hydrolysis. The myosin subfragment-1, which cannot bind g2 light chain, liberated 0.85-1.16 mol of acetate per mol. On the other hand, negligible amounts of acetate could be detected from g1 and g2 light chains. The acetates, which were measured by a microenzymic method, were derived from the acetylated N-terminal of the heavy chain. It was concluded that g2 light chain is bound to the C-terminal region of subfragment-1, which is the link between the heads and tail of myosin.
mesh_terms: D000085:Acetates / Q000032:analysis; D000107:Acetylation; D000818:Animals; D006868:Hydrolysis; D046911:Macromolecular Substances; D009132:Muscles / Q000032:analysis; D009206:Myocardium / Q000032:analysis*; D009218:Myosins; D011485:Protein Binding; D011817:Rabbits; D013552:Swine
chemical_list: D000085:Acetates; D046911:Macromolecular Substances; D009218:Myosins