title: Bacteriophage T7 DNA replication in vitro. Electron micrographic analysis of T7 DNA synthesized with purified proteins.
abstract: Extensive replication of duplex T7 DNA is catalyzed in reactions contining T7 DNA polymerase, T7 gene 4 protein, and T7 RNA polymerase. When the product of this reaction is analyzed in the electron microscope, many eye form and Y form replication intermediates are observed. Replication in vitro is not initiated at a single region of the T7 genome. However, we tentatively conclude that initiation does occur preferentially at a few specific sites along the DNA, and that these sites may be near promoters at which the T7 RNA polymerase initiates transcription.
mesh_terms: D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D008854:Microscopy, Electron; D009691:Nucleic Acid Denaturation; D013604:T-Phages / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase