Total-field electron-microscopic examination of thin tissue slices via surface-embedding and multiple-mesa technics.
Total-field electron-microscopic examination of tissue slices of 50--200 micrometers thickness, used in various prestaining methods, can be accomplished by combining the following two technics: surface-embedding, a process in which the thin tissue slice remains on one plane during embedding, so that its entire cross section can be obtained; multiple-mesa technic, a trimming process to obtain multiple targets for examination on the same field. The two technics are described in detail.
D006801:Humans; D008854:Microscopy, Electron; D008867:Microtomy / Q000379:methods*