Raman spectra of normal and ultraviolet-induced cataractous rabbit lens.
The Raman spectra of a normal rabbit lens have been recorded over the range of 0 to 4000 cm-1. Spectral results indicate that protein conformation is predominantly beta-pleated sheet. Polarization measurements and assignments for most of the bands have been made; the spectral region for water indicates extensive hydrogen bonding. An N2 laser operating at 337 nm was used to produce cataracts in a rabbit lens; the Raman spectra of the cataractous lens were also recorded. Spectral changes are observable in the water region, and these are correlated with a mechanism of ultraviolet cataractogenesis.
D000818:Animals; D001834:Body Water / Q000032:analysis; D002386:Cataract / Q000209:etiology / Q000503:physiopathology*; D005136:Eye Proteins / Q000032:analysis; D007834:Lasers / Q000009:adverse effects* / Q000592:standards; D007908:Lens, Crystalline / Q000032:analysis*; D011817:Rabbits; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D014466:Ultraviolet Rays / Q000009:adverse effects
D005136:Eye Proteins