Radiolabeling of affinity-purified goat anti-carcinoembryonic antigen immunoglobulin G with technetium-99m.
Affinity-purified goat anti-carcinoembryonic antigen immunoglobulin G was labeled with 99mTc, utilizing stannous tartrate as the reducing agent. The radiochemical yield of labeled antibody ranged from 18 to 37% and was inversely proportional to the initial quantity of pertechnetate ion used. Yields up to 60% could be obtained using small amounts of pertechnetate. The 99mTc-labeled anti-carcinoembryonic antigen immunoglobulin G preparations had a specific activity range of 1 to 6 microCi/microgram protein and had 60% or greater immunoreactivity. Human serum albumin and goat immunoglobulin G were used as model systems to evaluate labeling parameters and analytical methods.
D000912:Antibodies, Neoplasm / Q000276:immunology; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D002850:Chromatography, Gel; D006207:Half-Life; D006801:Humans; D007074:Immunoglobulin G; D007553:Isotope Labeling / Q000379:methods*; D011877:Radionuclide Imaging / Q000379:methods; D012709:Serum Albumin; D013667:Technetium
D000912:Antibodies, Neoplasm; D002272:Carcinoembryonic Antigen; D007074:Immunoglobulin G; D012709:Serum Albumin; D013667:Technetium