The effects of tri-iodothyronine on the synthesis of mitochondrial proteins in isolated rat hepatocytes.
The effects of tri-iodothyronine on mitochondrial protein synthesis have been studied in in vitro labeled, isolated rat hepatocytes. Hepatocytes were isolated from hypothyroid rats or from hypothyroid rats 24 h after injecting a single, low dose of hormone (20-30 microgram/180-230 g body weight). Tri-iodothyronine increased translation on mitochondrial ribosomes by 2-3-fold, but, under our conditions, appears to have little or not effect on the general synthesis of cytoplasmically-translated mitochondrial proteins. Electrophoretic and fluorographic analysis indicated that tri-iodothyronine stimultes labeling of the four major mitochondrially-translated peptides. The hormone appears to act by inducing a general increase in translation/transcription of mitochondrially-synthesized peptides.
D000818:Animals; D004272:DNA, Mitochondrial / Q000378:metabolism; D007037:Hypothyroidism / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D008713:Methimazole; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects; D051381:Rats; D014284:Triiodothyronine / Q000494:pharmacology* / Q000627:therapeutic use
D004272:DNA, Mitochondrial; D014284:Triiodothyronine; D008713:Methimazole