title: The protein pattern of PMF virus, a type-D retrovirus from malignant permanent human cell lines.
abstract: The protein pattern of a type-D retrovirus (PMFV) isolated from and propagated in human cell lines has been investigated using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Staining with Coomassie blue and labeling with 14C-leucine/14C-lysine revealed five viral polypeptides with molecular weights of 10,000, 12,000, 15,000, 25,000, and 68,000. The 68,000 D-protein was shown to be a glycoprotein by incorporation of 3H-glucosamine and the 15,000 D-protein was identified as a phosphoprotein. By comparing PMFV with the closely related Mason-Pfizer monkey virus (MPMV) in co-electrophoresis experiments no clear difference was detected in viral 14C-leucine/14C-lysine profiles. The viruses differ, however, with respect to their glycoprotein patterns. A glycoprotein corresponding to the gp20 of MPMV has not been detected in PMFV irrespective of the cell line used for propagation of viruses.
mesh_terms: D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008970:Molecular Weight; D010051:Ovarian Neoplasms; D010455:Peptides / Q000032:analysis; D010750:Phosphoproteins / Q000032:analysis*; D012190:Retroviridae / Q000032:analysis* / Q000302:isolation & purification; D012208:Rhabdomyosarcoma; D014764:Viral Proteins / Q000032:analysis*
chemical_list: D006023:Glycoproteins; D010455:Peptides; D010750:Phosphoproteins; D014764:Viral Proteins