title: Control of adenovirus early gene expression: a class of immediate early products.
abstract: We have identified the viral mRNAs present in cells in which protein synthesis has been stringently inhibited prior to infection with adenovirus type 2. These species presumably represent the subset of viral mRNAs that are "immediate early" products, requiring only host cell genes of their expression, and they do not include any of the conventionally recognized early mRNAs. Treatment of cells with 100 microM anisomycin inhibits 99.6% of protein synthesis and substantially depresses (by 20--200 fold) the levels of the conventional early mRNAs from regions E1A, E1B, E2, E3 and E4. Also depressed are species encoding an 87K protein (11.6--31.5 map units) and a 13.6K protein (encoded a short distance to the right of 21.5 map units). The only mRNAs not depressed by this treatment are an mRNA for a 13.5K protein encoded between 17.0 and 21.5 map units, and the mRNA for the late 52,55K protein encoded between 29 and 34 map units, which is also present in small amounts at early times. Further proof that production of the mRNA for the immediate early 13.5K protein is independent of E1A gene function is provided by the observation that it can be detected in cells infected with the E1A deletion mutant dl312.
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics* / Q000378:metabolism; D000841:Anisomycin / Q000494:pharmacology; D005814:Genes, Viral; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D000841:Anisomycin