An improved three-step method for the purification of the third component of human complement.
This paper describes a three-step purification method for the third component of human complement (C3) from plasma. The method consists of PEG precipitation, DEAE-cellulose chromatography and preparative isofocusing in a granulated dextran gel, with pH range 5--8. From this last step, a highly purified form of native C3 was obtained as indicated by immunoelectrophoresis and polyacrylamide gel electrophoresis analysis. The average final recovery was 20%.
D001665:Binding Sites; D002848:Chromatography, DEAE-Cellulose; D003176:Complement C3 / Q000302:isolation & purification*; D006801:Humans; D007525:Isoelectric Focusing; D011092:Polyethylene Glycols / Q000494:pharmacology
D003176:Complement C3; D011092:Polyethylene Glycols