[A single reagent for the enzymatic determination of urinary and blood urea (author's transl)].
An automatic continuous flow method is described for assay of plasmatic and urinary urea after hydrolysis by urease followed by NH4 assay with glutamic deshydrogenase in the same working solution. Precision and accuracy, comparison with chemical diacetylmonoxim method are studied. The interaction of endogenous NH4, the cost of the method are calculated.
D001322:Autoanalysis; D005069:Evaluation Studies as Topic; D005969:Glutamate Dehydrogenase; D000644:Quaternary Ammonium Compounds; D014508:Urea / Q000032:analysis* / Q000097:blood / Q000652:urine; D014510:Urease
D000644:Quaternary Ammonium Compounds; D014508:Urea; D005969:Glutamate Dehydrogenase; D014510:Urease
Utilisation d'un réactif unique pour le dosage enzymatique de l'urée sanguine et urinaire.