[P1, P3-(5'-guanosyl-5''-[14C]adenosyl)triphosphate: preparation of the cap parent compound and its catabolic properties towards rat liver subcellular fractions (author's transl)].
We report on the chemical preparation of 14C-labeled P1, P3-(5'-guanosyl-5''-adenosyl)triphosphate (1) which is the parent compound of 5'-terminal cap structures of most eukaryotic mRNAs. Incubation experiments of cap (1)- and non cap-structured nucleotides as Ap2A (2) as well as their ribose ring opened derivatives (3, 4) with rat liver subcellular fractions show that cap-degrading nucleases being able to unblock 5' termini are present in nuclei but not in mitochondria.
D000818:Animals; D002250:Carbon Radioisotopes; D002467:Cell Nucleus / Q000378:metabolism; D015226:Dinucleoside Phosphates; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D008722:Methods; D008930:Mitochondria, Liver / Q000378:metabolism; D012315:RNA Caps / Q000138:chemical synthesis* / Q000378:metabolism; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D002250:Carbon Radioisotopes; D015226:Dinucleoside Phosphates; D012315:RNA Caps; C017354:guanosine 5'-triphosphate-5'-adenosine; D006160:Guanosine Triphosphate
P1, P3-(5'-Guanosyl-5''-[14C]adenosyl)triphosphat: Darstellung der Kappenmutterverbindung sowie deren Abbauverhalten gegenüber subzellulären Fraktionen der Rattenleberzelle.