["Diaminobenzidine black" as a new histochemical demonstration of exogenous iron (author's transl)].
A new reaction for the histochemical demonstration of exogenous iron, used as a tracer for the study of connectivity in the central nervous system, is described. It consists of, first, the conversion of iron into Prussian blue, which acts secondarily as a catalyst for the oxidation of diaminobenzidine by hydrogen peroxide. The oxidized diaminobenzidine precipitates by polymerization and gives rise to a brown-colored insoluble reaction product. This reaction has a strikingly better sensitivity and contrast than the classical Perls's reaction.
D015100:3,3'-Diaminobenzidine; D000818:Animals; D001560:Benzidines; D002384:Catalysis; D002490:Central Nervous System / Q000378:metabolism*; D006651:Histocytochemistry; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000032:analysis* / Q000494:pharmacology; D010084:Oxidation-Reduction; D011048:Polarography; D011540:Prussian Blue Reaction; D051381:Rats
D001560:Benzidines; D015100:3,3'-Diaminobenzidine; D006861:Hydrogen Peroxide; D007501:Iron
"Noir de diaminobenzidine": une nouvelle méthode histochimique de révélation du fer exogène.