In vitro induction of SCE in human lymphocytes by epichlorohydrin with and without metabolic activation.
Human lymphocytes cultured for 73 h in the presence of BrdU were exposed to epichlorohydrin (ECH) with and without metabolic activation. Cells exposed for the entire culture time without activation showed a significant dose related increase in SCE yield. Exposure for the final 25 h only showed a similar response but with increased tolerance. The presence of S9 mix significantly reduced the effect of ECH compared with a similar exposure without activation. Without activation 4 X 10(-4) M ECH produced a 3-fold increase in the incidence of SCE over the control level. However, with S9 mix a concentration of 10(-3) M ECH was required to produce such an increase indicating that S9 mix may have a detoxifying effect.
D000818:Animals; D001711:Biotransformation; D002728:Chlorohydrins / Q000494:pharmacology*; D002842:Chromatids / Q000187:drug effects*; D003434:Crossing Over, Genetic; D004305:Dose-Response Relationship, Drug; D004811:Epichlorohydrin / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008862:Microsomes, Liver / Q000378:metabolism; D009153:Mutagens; D051381:Rats; D012854:Sister Chromatid Exchange
D002728:Chlorohydrins; D009153:Mutagens; D004811:Epichlorohydrin