Size increase induced in Walker ascites cells by chemotactic factors.
Upon interaction with the tumor cell chemotactic factor derived from zymosan-activated serum or with the synthetic chemotactic factor, formylmethionyl-leucyl-phenylalanine (f-met-leu-phe), the number of Walker 256 carcinosarcoma cells in suspension dropped as measured by a Coulter Counter. The drop in the number of cells counted by the Coulter Counter was accompanied by and possibly attributed to a swelling in the mean cell volume of approx. 20%. These effects were blocked by the addition of 2-deoxy-d-glucose, along with the chemotactic factors, to the tumor cells. The doses of the chemotactic factors which induced these responses paralleled closely the doses which have been shown to induce a chemotactic response as measured in the Boyden Chamber assay. These findings indicate that non-leukocytic cells, like leukocytes, are capable of other responses in addition to chemotactic migration in the Boyden chamber assay upon interaction with appropriate chemotactic factors.
D000818:Animals; D002279:Carcinoma 256, Walker / Q000276:immunology / Q000473:pathology*; D002449:Cell Aggregation / Q000187:drug effects; D002452:Cell Count; D002630:Chemotactic Factors / Q000494:pharmacology*; D003182:Complement C5 / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000276:immunology; D051381:Rats
D002630:Chemotactic Factors; D003182:Complement C5