[Na+ and Cl- ion concentration gradients between the external medium and tissues of the rabbit gallbladder wall].
It was shown in experiments on isolated gallbladders of rabbits that after 2 hours of incubation in Krebs solution, the concentration of Na+ and Cl- in mucous solutions decreased by 9.6 +/- 3.1 mM and 14.8 +/- 4.3 mM, respectively. Stereomicroscopy showed, under scraping of the epithelial layer of the gallbladder, a lucid intercellular fluid that could be collected in an amount of 2--4 mkl. The concentration of Na+ in this liquid was equal to 220.8 mM and that of Cl- amounted to 218.6 mM, the mean rate of the gallbladder fluid absorption being 43 mkl/h per 100 mg of the wet gallbladder wall weight. The concentration of the ions in intercellular fluid was inversly proportional to the volumetric epithelial flow rate. Na+ and Cl- concentration in the intercellular fluid was 20--25 mM higher than in incubation medium, with the absorption rate reaching 60 mkl/h per 100 mg of the wet gallbladder wall weight. The results obtained confirm the Diamond hypothesis of the existence of hyperosmotic area in the gallbladder wall.
D000042:Absorption; D000818:Animals; D001692:Biological Transport; D002712:Chlorides / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism; D005704:Gallbladder / Q000378:metabolism*; D011817:Rabbits; D012964:Sodium / Q000378:metabolism*
D002712:Chlorides; D012964:Sodium
Kontsentratsionnye gradienty ionov Na+ i Cl-mezhdu naruzhnoĭ sredoĭ i tkaniami stenki zhelchnogo puzyria krolika.