Fully enzymic method of plasma triglyceride determination using an immobilized glycerol dehydrogenase nylon-tube reactor.
A new, enzymic method of triglyceride determination in serum and plasma by use of an immobilized glycerol dehydrogenase nylon-tube reactor, integrated into the flow system of an AutoAnalyzer II (Technicon) is described. The combination of this reactor, stable for 1500--2000 tests, with the lipolytic enzymes which are added in solution yields a reliable and reproducible assay, which correlates well with the commonly used fully enzymic triglyceride determination. Using this new method, the cost can be reduced to about one-third of that of the other method.
D000818:Animals; D001322:Autoanalysis / Q000379:methods; D004800:Enzymes, Immobilized; D013401:Sugar Alcohol Dehydrogenases; D014280:Triglycerides / Q000097:blood*
D004800:Enzymes, Immobilized; D014280:Triglycerides; D013401:Sugar Alcohol Dehydrogenases; C020672:glycerol dehydrogenase