title: [Thermodynamic study of guanylspecific ribonuclease C2].
abstract: Guanylspecific RNAse C2 has been studied thermodynamically using a microcalorimetry technique. It is shown that at ph varying from 5,0 to 8,0 the RNAse C2 solution in a cacodylate buffer contains about 30% of monomeric and about 70% of dimeric forms of protein, each of them melting as a single cooperative system. Moreover, studies of the influence of phosphate ions have shown that they stabilize the system by 3-4 degrees and transform it into a monomeric form.
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D002152:Calorimetry, Differential Scanning; D006863:Hydrogen-Ion Concentration; D011489:Protein Denaturation; D012260:Ribonucleases; D013696:Temperature; D013816:Thermodynamics
chemical_list: D012260:Ribonucleases
vernacular_title: Termodinamicheskoe issledovanie guanilspetsifichnoĭ ribonukleazy C2.