Separation of serum prednisolone and prednisolone-21-hemisuccinate by extraction and their concurrent determination by radioimmunoassay.
We describe a simple, sensitive, and reliable radioimmunoassay for prednisolone and prednisolone-21-hemisuccinate in serum. The antiserum produced in rabbits to prednisolone-21-hemisuccinate/bovine serum albumin was specific for prednisolone and prednisolone-21-hemisuccinate. A simple dichloromethane extraction permitted the separation of prednisolone from prednisolone-21-hemisuccinate in the serum samples. Interference by cortisol, although not insignificant, is minimized in this assay. We used the method to measure prednisolone and prednisolone-21-hemisuccinate concentrations after a bolus injection of prednisolone-21-hemisuccinate into human beings and mice.
D000818:Animals; D002561:Cerebrovascular Disorders / Q000097:blood; D003429:Cross Reactions; D006801:Humans; D008297:Male; D051379:Mice; D011239:Prednisolone / Q000031:analogs & derivatives* / Q000097:blood* / Q000302:isolation & purification; D011863:Radioimmunoassay / Q000379:methods
D011239:Prednisolone; C021322:prednisolone hemisuccinate