Determination of mulecular-weight distribution of aorta glycosaminoglycans by automated gel filtration.
This report describes an automated gel filtration procedure for estimation of molecular weights of glycosaminoglycans using Technicon sugar analyzer. A Sepharose CL-6B column was used for filtration. The method has been applied to determining the distribution of molecular weights of glycosaminoglycans from human aorta. The glycosaminoglycans were fractionated on a Dowex 1 (Cl-) column prior to gel filtration. On gel filtration, hyaluronic acid resolved into two components with estimated molecular weights of 38,000 and 8000. A molecular weight of 22,000 was estimated for heparan sulfate. Chondroitin 6-sulfate and dermatan sulfate eluted from the column in one peak, suggesting these two glycosaminoglycans have a similar molecular weight, 18,000. The procedure was found suitable for studying the gel filtration behavior of proteoglycans from bovine aorta and lung.
D000818:Animals; D001011:Aorta / Q000032:analysis*; D002417:Cattle; D002850:Chromatography, Gel / Q000379:methods; D006025:Glycosaminoglycans / Q000032:analysis*; D006801:Humans; D008168:Lung / Q000032:analysis; D008970:Molecular Weight
D006025:Glycosaminoglycans