Induction of contact sensitivity to DNCB with in vitro dinitrophenylated lymph node cells.
Contact sensitization was achieved with dinitrophenylated autologous lymph node cells killed by hypotonic lysis or sonication as well as live cells. The sensitizing ability of the sonicated cells disappeared after freeze-thawing or heating. The significance of these findings is discussed.
D000818:Animals; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology*; D004136:Dinitrobenzenes / Q000276:immunology*; D004137:Dinitrochlorobenzene / Q000276:immunology*; D006168:Guinea Pigs; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008297:Male; D009578:Nitrobenzenes / Q000276:immunology*
D004136:Dinitrobenzenes; D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes