Secretion of newly synthesized proteins by isolated liver cells.
Suspensions of isolated liver cells were incubated with [3H]leucine as a precursor. The appearance of radioactive albumin and alpha 1-acid glycoprotein in the incubation medium was determined. After about 20 min both albumin and alpha 1-acid glycoprotein were secreted in a linear way. When fucose and galactose were used as radioactive precursors, time lags of about 10 min could be observed.
D000418:Albumins / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D005643:Fucose / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis / Q000378:metabolism; D066298:In Vitro Techniques; D007930:Leucine; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D013997:Time Factors
D000418:Albumins; D006023:Glycoproteins; D011506:Proteins; D005643:Fucose; D007930:Leucine; D005690:Galactose