Sindbis virus RNA replication. II. Strand composition and metabolic fate of the multi-stranded RNA species.
Double-stranded RNA from SB virus-infected cells was denatured and analysed on agarose-methylmercuric hydroxide gels. Equimolar amounts of three single-stranded species with mol. wt. of 4 x 10(6), 2.5 x 10(6) and 1.8 x 10(6) were found. Pulse and chase experiments in infected cells established a precursor-product relationship between the multi-stranded and single-stranded virus RNA species. The present results support the model in which the 49S and 26S species of virus RNA are synthesized in infected cells from two distinct replicating structures.
D007700:Kinetics; D008954:Models, Biological; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D012260:Ribonucleases / Q000494:pharmacology; D012845:Sindbis Virus / Q000378:metabolism*
D012367:RNA, Viral; D012260:Ribonucleases