Transitional states of the neuronal serotonergic site.
Binding of [3H]5-HT was studied using purified neuronal membranes isolated from horse brain striatum. It is shown that the preincubation of these membranes with an agonist (5-HT, bufotenin) followed by washing, modified the binding of [3H]5-HT from a low affinity to a high affinity constant. This effect was blocked by previous pretreatment of the membranes with a sulfhydryl group reagent (N-ethylmaleimide) and was reversed by guanoside 5'-triphosphate.
D000818:Animals; D001665:Binding Sites; D005033:Ethylmaleimide / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006736:Horses; D008968:Molecular Conformation; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*; D013997:Time Factors; D014316:Tritium
D011985:Receptors, Serotonin; D014316:Tritium; D012701:Serotonin; D006160:Guanosine Triphosphate; D005033:Ethylmaleimide