Somatomedin activity in sera of genetically small mice.
Somatomedin activity as determined by the stimulation of [3H]thymidine incorporation into chick embryo fibroblast DNA, was measured in extracts of sera from genetically small mice. Somatomedin activity was low in the sera from growth hormone deficient mice [Snell (dw/dw), Ames (df/df), and little (lit/lit)] whereas somatomedin activity was normal in serum from the pygmy (pg/pg)) mouse, a mutant proposed as a model for growth hormone resistance. GH treatment of the Snell mouse resulted in an increase in somatomedin activity to normal levels.
D000818:Animals; D001681:Biological Assay; D002478:Cells, Cultured; D002642:Chick Embryo; D002850:Chromatography, Gel; D004261:DNA Replication / Q000187:drug effects; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D013006:Growth Hormone / Q000172:deficiency / Q000494:pharmacology; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains / Q000097:blood*; D013002:Somatomedins / Q000097:blood*; D013936:Thymidine / Q000378:metabolism
D013002:Somatomedins; D013006:Growth Hormone; D013936:Thymidine