title: A steady state and differential polarised phase fluorimetric study of the liver microsomal and mitochondrial membranes of thermally acclimated green sunfish (Lepomis cyanellus).
abstract: The liver mitochondrial and microsomal membranes of green sunfish and rat were examined by steady state polarisation and differential polarised phase fluorimetry to determine the effects of seasonal adaptation of membrane dynamic structure to temperature. Steady state polarisation studies indicated that the liver mitochondria of green sunfish acclimated to different temperatures showed a greater partial compensation of membrane fluidity for the fatty acid composition of both membrane preparations generally became more unsaturated at lower acclimation temperatures, though the differences between 5 degrees C and 25 degrees C acclimated fish were more pronounced in the mitochondrial fraction than in the microsomal fraction. Differential polarised phase fluorimetric studies indicated that the rotations of diphynylhexatriene in mitochondrial and microsomal membranes were highly hindered, though the hindrance offered by membranes of 25 degrees C acclimated green sunfish was far greater than that offered by the membranes of 5 degrees C acclimated fish, thus supporting the concept of homeoviscous adaptation. The absolute rotational rate was not consistently affected by acclimation treatment.
mesh_terms: D000064:Acclimatization; D000818:Animals; D005227:Fatty Acids / Q000032:analysis; D005399:Fishes; D007425:Intracellular Membranes / Q000648:ultrastructure*; D008563:Membrane Lipids / Q000032:analysis*; D008862:Microsomes, Liver / Q000648:ultrastructure*; D008930:Mitochondria, Liver / Q000648:ultrastructure*; D010743:Phospholipids / Q000032:analysis*; D051381:Rats; D012621:Seasons; D013045:Species Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D005227:Fatty Acids; D008563:Membrane Lipids; D010743:Phospholipids