The effect of prostaglandins on sulphate and thymidine incorporation in pig cartilage.
Prostaglandins A1, A2, B2, E2, and F2 alpha inhibit glycosaminoglycan sulphation in pig costal cartilage discs above 0.1 micrometer M (5-25 ug/ml); no effects were found at concentrations down to 25 pg/ml. At concentrations above 1 microgram/ml (0.02 mM) prostaglandin E2 and F2 alpha inhibited cartilage DNA synthesis. However at 100 ng/ml (10 microM) this inhibition was again observed. The basal production of prostaglandins E2 and F2 alpha by the cartilage discs after incubation was below 15 pg/ml. The exact concentrations at which these inhibitory effects were observed varied from experiment (i.e. animal) to experiment.
D000818:Animals; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D011453:Prostaglandins / Q000494:pharmacology*; D011454:Prostaglandins A / Q000494:pharmacology; D011456:Prostaglandins B / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D013431:Sulfates / Q000378:metabolism*; D013552:Swine; D013936:Thymidine / Q000378:metabolism*
D006025:Glycosaminoglycans; D011453:Prostaglandins; D011454:Prostaglandins A; D011456:Prostaglandins B; D011458:Prostaglandins E; D011460:Prostaglandins F; D013431:Sulfates; D004247:DNA; D013936:Thymidine