Estriol-induced dose-dependent DNA synthesis in normal human mammary epithelium in vitro.
Normal human adult resting mammary gland explants were cultured during 7 days. The influence of hormones on DNA synthesis was assessed by autoradiography after incubation with tritiated-thymidine. Estriol was added to the medium at 3 different concentrations. With 0.01 ng/ml the labeling index remained at the level observed in the controls in the basic unenriched medium. A 10- and 100-fold increase of estriol elicited a transient, significant and dose-related increase of DNA synthesis on the 2nd day of organ culture. Estriol gave a qualitatively different profile of DNA synthesis than estradiol at 1.0 ng/ml. The results are compared with the effect of progesterone.
D001940:Breast / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004964:Estriol / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009924:Organ Culture Techniques; D011374:Progesterone / Q000494:pharmacology; D013268:Stimulation, Chemical; D013997:Time Factors
D011374:Progesterone; D004247:DNA; D004964:Estriol