Structural study of the carbohydrate moiety of bovine pancreatic ribonuclease B.
The sugar chain of bovine pancreatic ribonuclease B was released from the polypeptide moiety by hydrazinolysis and by endo-beta-N-acetylglucosaminidase H digestion, and reduced with NaB[3H]4 after N-acetylation. The radioactive oligosaccharide mixtures thus obtained were fractionated by Bio-Gel P-4 column chromatography, and their structures were studied by sequential exoglycosidase digestion, methylation study, periodate oxidation and acetolysis. The results show that bovine pancreatic ribonuclease B has one of the following sugar chains as asparagine-linked carbohydrate. Formula (See Text).
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002417:Cattle; D006026:Glycoside Hydrolases; D008745:Methylation; D009844:Oligosaccharides / Q000032:analysis; D010179:Pancreas / Q000201:enzymology*; D012260:Ribonucleases
D009844:Oligosaccharides; D012260:Ribonucleases; D006026:Glycoside Hydrolases