[Isolation and purification of various extracellular ribonucleases from Penicillium brevi-compactum in a single technological cycle].
The possibility of producing nonspecific acid and guanyl-specific alkaline ribonucleases of Penicillium brevi-compactum in a single technological cycle was explored. The use of pre-purification of anion exchanger EDE-10P and strongly basic anion exchanging fiber CM-A2 at an initial stage produced a solution of enzymes, from which both enzymes in a pure form and with a high yield were isolated, using CM-cellulose and Sephadex G-75 chromatography. Both enzymes proved homogeneous with respect to protein.
D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D003460:Crystallization; D010407:Penicillium / Q000201:enzymology*; D012260:Ribonucleases / Q000302:isolation & purification*
D012260:Ribonucleases
Vydelenie i ochistka preparatov razlichnykh vnekletochnyky nibnukleaz Brevi-compactum v edinom tekhnologicheskom tsikle.