title: Phospholipid synthesis and exchange between rat liver microsomes and mitochondria in the presence of benzo(a)pyrene.
abstract: Benzo(a)pyrene injection increased the phospholipid content in membranes of rat liver mitochondria and microsomes. There was a large relative increase of phosphatidylcholine, especially in microsomes, as compared with normal liver. The quantity of phosphatidylethanolamine seemed not be affected and the other phospholipid classes decreased. In vivo [U-14C]glycerol incorporation into phospholipids was greater after benzo(a)pyrene injection, especially into phosphatidylcholine. Liver microsomes derived from rats injected with [U-14C]glycerol or from liver slices incubated with the same precursor were incubated with unlabeled mitochondria in the presence of 105,000 g supernatant fraction; labeled microsomal phospholipids exchanged to a greater extent in the presence of benzo(a)pyrene, suggesting either a stimulation of activity of the exchange proteins, or a relative membrane disorganization facilitating the phospholipid transfer.
mesh_terms: D000818:Animals; D001580:Benzopyrenes / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D013997:Time Factors
chemical_list: D001580:Benzopyrenes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids