Separation of barbiturates using reverse-phase thin-layer chromatography.
A procedure for separating barbiturates by reverse-phase TLC is described. A spotted TLC plate is dipped in mineral oil/petroleum ether (1:10) and run 15 cm in a solvent of water/methanol/ammonium hydroxide, (80:20:2). After visualization, the drugs tested showed distinct separation.
D001463:Barbiturates / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods*
D001463:Barbiturates