Determination of hydralazine metabolites: 4-hydrazino-phthalazin-1-one and n-acetylhydrazinophthalazin-1-one by gas chromatography and s-triazolo[3,4-alpha]phthalazine and phthalazinone by high-performance liquid chromatography.
Methods are described for the determination of 2-N-acetylhydrazinophthalazin-1-one, 4-hydrazinophthalazin-1-one, phthalazinone and s-triazolo[3,4-alpha]phthalazine in human urine. 4-Hydrazinophthalazin-1-one and 4-N-acetylhydrazinophthalazin-1-one (following acid hydrolysis) are reacted with acetylacetone to give a distinctive pyrazole derivative which can be determined by gas chromatography using a nitrogen-specific detector. Phthalazinone and s-triazolo[3,4-alpha]phthalazine are measured  underivatised by high-performance liquid chromatography.
D002849:Chromatography, Gas / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D006830:Hydralazine / Q000378:metabolism*; D010793:Phthalazines / Q000652:urine*; D011724:Pyridazines / Q000652:urine*
D010793:Phthalazines; D011724:Pyridazines; D006830:Hydralazine