Determination of methimazole in plasma using gas chromatography--mass spectrometry after extractive alkylation.
A gas chromatography--mass spectrometry method for quantitation of the thyreostatic agent methimazole in plasma is described. The drug was transferred from the plasma sample and derivatized in one step by extractive alkylation. Either of two alkylating agents benzyl-chloride or pentafluorobenzyl bromide were used. Deuterium-labelled methimazole was used as internal standard. The precision of the method at the level of 5 ng methimazole per ml plasma was 6%.
D000478:Alkylation; D001593:Benzyl Compounds; D005464:Fluorobenzenes; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008713:Methimazole / Q000097:blood*; D005466:Fluorocarbons
D001593:Benzyl Compounds; D005464:Fluorobenzenes; D008713:Methimazole; C025063:pentafluorobenzyl bromide; D005466:Fluorocarbons; C021292:benzyl chloride