Development of a cell-free protein-synthesizing system from Schistosoma mansoni and a comparison of the effects of hycanthone and praziquantel on this system.
A cell-free, protein-synthesizing system was developed using polysomes and pH 5 fractions isolated from Schistosoma mansoni. Under optimal conditions, the system incorporated 35-S methionine into TCA-precipitable protein at a rate of approximately 1.8 pmoles/hr/microgram polysomal RNA. Praziquantel had no significant effect on the rate of protein synthesis, whereas hycanthone, at concentrations of 0.125 and 0.5 mM, caused a 136% and 515% stimulation, respectively. The mechanism of the stimulation is unknown. This S. mansoni--cell-free system for protein synthesis has been standardized so that new drugs can now be screened with relative ease for their effects on in vitro protein synthesis or charging of tRNA.
D000818:Animals; D002474:Cell-Free System; D006826:Hycanthone / Q000494:pharmacology*; D007546:Isoquinolines / Q000494:pharmacology*; D007700:Kinetics; D011132:Polyribosomes / Q000378:metabolism; D011223:Praziquantel / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000378:metabolism; D012550:Schistosoma mansoni / Q000378:metabolism*; D013892:Thioxanthenes / Q000494:pharmacology*
D007546:Isoquinolines; D013892:Thioxanthenes; D006826:Hycanthone; D011223:Praziquantel; D012343:RNA, Transfer