Histamine-induced decrease of membrane-bound calcium ions in the membrane fraction of rabbit taenia coli.
The difference between the interactions of acetylcholine and those of histamine with membrane-bound Ca2+ was studied. Plasma membrane vesicles were prepared from two types of smooth muscle of rabbit intestine: one sensitive to histamine and the other insensitive. The membrane vesicles were incubated with Ca2+ and bound Ca2+ was measured fluorometrically with chlorotetracycline. Acetylcholine did not affect the fluorescence, whereas histamine decreased it in preparations sensitive to histamine but not in those insensitive to histamine.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002751:Chlortetracycline; D005470:Fluorometry; D006632:Histamine / Q000494:pharmacology*; D007422:Intestines / Q000187:drug effects; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism*; D010539:Permeability; D011817:Rabbits
D006632:Histamine; D000109:Acetylcholine; D002118:Calcium; D002751:Chlortetracycline