Cytochemistry of mouse mast cell reaction to polylysine.
The modifications of mouse mast cells incubated in solutions of polylysine at two different concentrations are studied. At higher dose (0.5 mg/ml), the polycation has evident toxic effects. At lower dose (0.5 micrograms/ml), it induces some modifications of the granule morphology. Cytochemical reactions to demonstrate polyanions, basic proteins and organic bases are used to study the granule content. A polylysine treatment promotes the release of basic groups (vaso-amines) and sulphydryl-containing compounds (basic protein) from the mast cell granules. On the contrary, polyanions remain in the cells and correspond to the granule matrix persisting after amino-liberator treatment.
D000588:Amines / Q000378:metabolism; D000818:Animals; D000838:Anions / Q000378:metabolism; D002843:Chromatin / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000187:drug effects* / Q000378:metabolism; D006651:Histocytochemistry; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D010455:Peptides / Q000494:pharmacology*; D011107:Polylysine / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism
D000588:Amines; D000838:Anions; D002843:Chromatin; D010455:Peptides; D011506:Proteins; D011107:Polylysine