Separation and quantitation of chlortetracycline, 4-epitetracycline, 4-epianhydrotetracycline, and anhydrotetracycline in tetracycline by high-performance liquid chromatography.
The analysis of tetracycline epimers in tetracycline preparations by high-performance liquid chromatography is described. The method uses a microparticulate phenyl column (3.9 mm i.d. X 30 cm) with a step gradient of 12-22% acetonitrile in 0.2 M phosphate buffer at pH 2.2. The analysis takes 22 min. The relative standard deviations of the method (2 sigma, n = 6) for the analysis of chlortetracycline, 4-epitetracycline, 4-epianhydrotetracycline, and anhydrotetracycline in tetracycline were +/- 3.68, +/- 4.47, +/- 7.60, and +/- 2.77 %, respectively.
D002214:Capsules / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002751:Chlortetracycline / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004340:Drug Contamination; D011208:Powders / Q000032:analysis; D013237:Stereoisomerism; D013752:Tetracycline; D013754:Tetracyclines / Q000032:analysis* / Q000302:isolation & purification
D002214:Capsules; D011208:Powders; D013754:Tetracyclines; C016229:4-epianhydrotetracycline; D013752:Tetracycline; D002751:Chlortetracycline