Simultaneous preparation of mononuclear and polymorphonuclear leucocytes from horse blood on Ficoll-Hypaque medium.
Results presented show that highly purified populations of mononuclear (MN) and polymorphonuclear (PMN) leucocytes can be obtained from horse blood by a procedure similar to that previously described for the separation of these leucocytes from human blood. This involved centrifugation of horse blood on a Ficoll-Hypaque medium with a density of 1.095 g/ml. The procedure required approximately 1 h for completion and resulted in the simultaneous preparation of MN (greater than 98% purity) and PMN (greater than 96% purity) leucocytes. Cell viability exceeded 95% and cells retained immunological functions.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D005362:Ficoll; D006736:Horses / Q000276:immunology; D008853:Microscopy; D009000:Monocytes / Q000145:classification* / Q000276:immunology; D009504:Neutrophils / Q000145:classification* / Q000276:immunology
D005362:Ficoll