title: Influence of uremic middle molecules on blood cells.
abstract: Uremic middle molecular weight substances are obtained from the ultrafiltrate of plasma of uremic patients. Seven subfractions are isolated by chromatography using Sephadex G-15 and DEAE 25 A columns. Each subfraction is tested for its inhibitory effect upon lymphocyte blastogenesis and influence upon osmotic fragility of red blood cells. Consequently, subfractions c and d exert notable effects on both lymphocytes and red blood cells. The main constituents of subfraction d are aspartic acid, glycine, glutamine, and cysteine. It is concluded that uremic middle molecular substances have a deleterious effect upon blood cells. Therefore, hemofiltration, which is advantageous for the removal of middle molecular weight substances, is considered to be a method of choice for uremic patients.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*; D009996:Osmotic Fragility / Q000187:drug effects; D014118:Toxins, Biological / Q000032:analysis / Q000097:blood / Q000494:pharmacology; D014462:Ultrafiltration; D014511:Uremia / Q000097:blood*
chemical_list: D000596:Amino Acids; D014118:Toxins, Biological