Release of Paramecium immobilization antigen to the non-nutrient medium.
The instability of Paramecium aurelia surface components has been shown: after 60 min incubation of dense cell suspension ((1-2 . 10(6) cells per ml) in Tris-Ca buffer at 4 degrees C or 23 degrees C the surface coat was partially stripped off and the proteins were released to the medium. The electrophoretic analysis of the released proteins shows one major band of mol. wt. 280,000--300,000 and some minor bands. The major released protein is the immobilization antigen as proved using immunodiffusion test and antibody precipitation technique.
D000818:Animals; D000954:Antigens, Surface; D005110:Extracellular Space / Q000276:immunology; D008124:Locomotion; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D008970:Molecular Weight; D010247:Paramecium / Q000276:immunology* / Q000648:ultrastructure
D000954:Antigens, Surface; D008565:Membrane Proteins