Contributionof Z-line constituents to the formation of the contraction bands of chicken myofibrils on addition of Mg2+-ATP.
1. Z-line constituents contributing to the formation of the contraction bands in chicken myofibrils were investigated by using the rabbit calcium-activated factor (CAF). 2. CAF digestion hampered the formation of the contraction bands and increased the amount of soluble proteins which are apparently derived from the Z-line. Among several proteins released by CAF digestion, a 95,000 dalton component, which corresponds to alpha-actinin, was predominant, followed by a 220,000 dalton component and several other proteins. 3. An apparent inverse relationship between the formation of the contraction bands and the release of Z-line proteins was observed. It is concluded that the presence of Z-line, including at least the two components mentioned above, is essential for the formation of the contraction bands.
D000185:Actinin / Q000502:physiology; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002645:Chickens; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D008297:Male; D008970:Molecular Weight; D009119:Muscle Contraction / Q000187:drug effects*; D009124:Muscle Proteins / Q000502:physiology*; D009132:Muscles / Q000187:drug effects / Q000502:physiology; D009210:Myofibrils / Q000502:physiology* / Q000648:ultrastructure; D011817:Rabbits
D009124:Muscle Proteins; D000185:Actinin; D000255:Adenosine Triphosphate; D008274:Magnesium