Purification of guanine deaminase inhibitor from human brain.
A procedure for the purification of guanine deaminase inhibitor from human brain mitochondria is described. The inhibitor was enriched about 150-fold with recoveries of over 65%. It is nondialyzable, insoluble in water, and stable for over 30 days at -16 degrees C. However, the protein is completely inactivated at 50 degrees C in 5 min. The purified protein also inhibits the activities of a number of other enzymes.
D000619:Aminohydrolases / Q000037:antagonists & inhibitors*; D001921:Brain / Q000201:enzymology*; D004355:Drug Stability; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000502:physiology; D006148:Guanine Deaminase / Q000037:antagonists & inhibitors*; D006801:Humans; D008928:Mitochondria / Q000502:physiology*
D004791:Enzyme Inhibitors; D000619:Aminohydrolases; D006148:Guanine Deaminase