The preparation of lipid-free extracts of drugs from blood for gas chromatographic analysis.
A method is presented to separate co-extracted biological materials, in particular lipids, from drugs in chloroform extracts of blood. The chloroform is evaporated and the residue is dissolved in a small volume of petroleum ether and partitioned with eight times this volume of 5% aqueous hydrochloric acid. The acidic, basic, and neutral drugs are extracted into the aqueous phase, leaving unwanted materials in the petroleum ether. The recoveries of drugs representative of a variety of types are presented. Reduction of the levels of lipids by factors on the order of 1000-fold is reported.
D002498:Centrifugation; D002725:Chloroform; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008055:Lipids; D004364:Pharmaceutical Preparations / Q000097:blood*
D008055:Lipids; D004364:Pharmaceutical Preparations; D002725:Chloroform