Acidic glycosaminoglycans in cultured skin fibroblasts of a patient with epidermolysis bullosa dystrophica dominans.
A biochemical study was carried out on acidic glycosaminoglycans in the cultured skin fibroblasts obtained from a patient with epidermolysis bullosa dystrophica dominans. Acidic glycosaminoglycans were separated from fibroblasts and medium, and subjected to quantiative and qualitative analyses by cellulose acetate membrane electrophoresis, column chromatography and gel filtration. It was found that hyaluronic acid and dermatan sulfate were slightly increased in epidermolysis bullosa dystrophica dominans in comparison with the normal control.
D002478:Cells, Cultured; D004820:Epidermolysis Bullosa / Q000378:metabolism* / Q000473:pathology; D005347:Fibroblasts / Q000032:analysis; D006025:Glycosaminoglycans / Q000032:analysis*; D006801:Humans; D012867:Skin / Q000032:analysis*
D006025:Glycosaminoglycans