Different divalent cation requirements for binding IgM complexes to lymphocytes and macrophages.
We have found that Mg++ supports IgM complex binding to mouse lymphocytes but not to macrophages. In contrast, Ca++ supports IgM complex binding to macrophages but not lymphocytes. IgG complex binding to both lymphocytes and macrophages is divalent cation independent. These findings allow one to distinguish IgG from IgM binding and macrophage from lymphocyte IgM complex binding by their differential divalent cation requirements.
D000818:Animals; D000936:Antigen-Antibody Complex; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology*; D002614:Chelating Agents / Q000494:pharmacology; D007074:Immunoglobulin G / Q000378:metabolism*; D007075:Immunoglobulin M / Q000378:metabolism*; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D011485:Protein Binding / Q000187:drug effects; D011971:Receptors, Immunologic / Q000378:metabolism
D000936:Antigen-Antibody Complex; D002413:Cations, Divalent; D002614:Chelating Agents; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011971:Receptors, Immunologic; D008274:Magnesium; D002118:Calcium