Studies on antitumor activity of prumycin. IV. Effect of prumycin on mouse immune system.
The effect of prumycin on the mouse immune system was studied. Prumycin at a dose of 75 mg/kg (1/2LD50) showed essentially no suppression of hemolytic plaque forming cells (PFC) against sheep red blood cells (SRBC) when it was administered on day 0, 1 or 2. However, a weak suppression was observed by administration of prumycin on day 3. Also a remarkable suppression of PFC was demonstrated when mouse spleen cells were pre-incubated with prumycin at the concentration over 62.5 mg/kg in vitro. The delayed-type hypersensitivity reaction against SRBC was remarkably suppressed by cyclophosphamide, but it was not much by prumycin, except by administration on the day of elicitation. Prumycin of a high concentration inhibited an uptake of 3H-thymidine by mouse spleen lymphocytes incubated with or without phytohemagglutinin, but that of a low concentration, 1.9 approximately 0.03 mcg/ml, slightly increased the blastogenic stimulation of lymphocytes.
D000606:Amino Sugars / Q000494:pharmacology; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000917:Antibody Formation / Q000187:drug effects; D001089:Arabinose / Q000031:analogs & derivatives / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D006968:Hypersensitivity, Delayed / Q000503:physiopathology; D007109:Immunity / Q000187:drug effects*; D007111:Immunity, Cellular / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology
D000606:Amino Sugars; D000903:Antibiotics, Antineoplastic; C025517:prumycin; D001089:Arabinose