[Use of the fluorescent probe 1-anilinonaphthalene-8-sulfonate to study the transmembrane potential of synaptosomes].
The properties of the transmembrane potential (TMP) of synaptosomes (Sin) from the rat brain cortex were studied by means of the fluorescent probe I-anilinonaphthalene-8-sulfonate (ANS). The fluorescent emission that increased at 464 nm (lambda ex = 365 nm) after addition of valinomycin to synaptosomes was considered as the reflection of Sin hyperpolarization. The reduction of K+-gradient across Sin membrane (decrease in TMP) induced by Sin preincubation with ouabain (1 mM) or by K+ increase from 5 to 20 mM noticeably decreased the effect of valinomycin. Valinomycin exerted no effect on Sin subjected to osmotic shock. It is suggested that valinomycin-induced changes in ANS fluorescence may be made use of for testing the ability of Sin obtained to retain K+-gradient (TMP presence), that is for testing the Sin nativity.
D000817:Anilino Naphthalenesulfonates; D000818:Animals; D002540:Cerebral Cortex / Q000648:ultrastructure*; D000431:Ethanol / Q000494:pharmacology; D066298:In Vitro Techniques; D008564:Membrane Potentials; D009997:Osmotic Pressure; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D013050:Spectrometry, Fluorescence; D013574:Synaptosomes / Q000187:drug effects / Q000502:physiology*; D014634:Valinomycin / Q000494:pharmacology
D000817:Anilino Naphthalenesulfonates; D014634:Valinomycin; D000431:Ethanol; D010042:Ouabain; D011188:Potassium
Primenenie fliuorestsentnogo zonda 1-anilinonaftalin-8-sul'fonata dlia izucheniia transmembrannogo potentsiala sinaptosom.