title: The effect of fluoride on the state of aggregation of adenylate cyclase in rat liver plasma membranes.
abstract: Irradiation inactivation was used to monitor changes in the state of adenylate cyclase in rat liver plasma membranes in the presence of F-.F- caused a decrease in the target size from 328000 to 237000 at 0 degrees C and from 329000 to 219000 at 30 degrees C. Adenylate cyclase was activated by F- at both 0 degrees C and 30 degrees C. The effect of F- was biphasic, activating up to a concentration of 10mM and inhibiting at higher concentrations. If adenylate cyclase weas maximally activated with glucagon and p[NH]ppG ([beta gamma-imido]GTP) all concentrations of F- were inhibitory. The implications of the results with respect to the mechanism of activation of adenylate cyclase are discussed.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005459:Fluorides / Q000494:pharmacology*; D005934:Glucagon / Q000494:pharmacology; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D046911:Macromolecular Substances; D051381:Rats; D012969:Sodium Fluoride / Q000494:pharmacology; D013696:Temperature
chemical_list: D046911:Macromolecular Substances; D006165:Guanylyl Imidodiphosphate; D012969:Sodium Fluoride; D005934:Glucagon; D000262:Adenylyl Cyclases; D005459:Fluorides