[Interaction of the enzyme xylonigrine with isolated cytoplasmatic membranes of cells of potato tubers Solanum tuberosum L].
The effect of the enzyme xylonigrine on isolated cytoplasmatic membranes of potato tuber cells was studied. Measurements of protein and carbohydrates in the supernatant following combined incubation and centrifugation as well as morphological characterization of microvesicles by electron microscopy demonstrated a lack of xylonigrine-induced morphological changes in microvesicles. A decrease in the protein quantity in the supernatant after incubation of membranes and xylonigrine for 2 hours at 20 degrees was found. As a result of further incubation for 6 hours the protein quantity approached the initial level. During incubation of membranes with xylonigrine for 6 hours at 40 degrees C the carbohydrate amount increased. The incubation for 2 and 6 hours at 20 degrees C did not cause any significant changes in the carbohydrate content in the supernatant.
D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis* / Q000648:ultrastructure; D006026:Glycoside Hydrolases; D008565:Membrane Proteins / Q000032:analysis*; D009097:Multienzyme Complexes / Q000378:metabolism*; D010447:Peptide Hydrolases; D010944:Plants / Q000032:analysis*
D002241:Carbohydrates; D008565:Membrane Proteins; D009097:Multienzyme Complexes; C024824:xylonigrine; D006026:Glycoside Hydrolases; D010447:Peptide Hydrolases
Vzaimodeĭstvie fermentnogo preparata ksilonigrina s izolirovannymi tsitoplazmaticheskimi membranami kletok klubnia kartofelia Solanum tuberosum L.