Peptide fragmentation suitable for solid-phase microsequencing. Use of N-bromosuccinimide and BNPS-skatole (3-bromo-3-methyl-2-[(2-nitrophenyl)thio]-3H-indole).
BNPS-skatole (3-bromo-3-methyl-2-(2-nitrophenyl)thiol-3H-indole) and N-bromosuccinimide have been used to specifically cleave at peptide bonds after amino acids with available C-gamma=C-delta double bonds, i.e. tryptophan, tyrosine and histidine. The resulting C-terminal lactones conveniently attach to amino-glass supports for sequencing with DABITC (4-NN-dimethylaminoazobenzene 4'-isothiocyanate). Also, peptides having such C-termini, i.e. from a chymotryptic digest, can be readily made to react with these reagents and thus be easily attached and sequenced by solid-phase methods.
D000595:Amino Acid Sequence; D001974:Bromosuccinimide; D002851:Chromatography, High Pressure Liquid; D006454:Hemoglobins; D006801:Humans; D007211:Indoles; D010446:Peptide Fragments; D012862:Skatole / Q000031:analogs & derivatives; D013388:Succinimides
D006454:Hemoglobins; D007211:Indoles; D010446:Peptide Fragments; D013388:Succinimides; C005904:BNPS-skatole; D012862:Skatole; D001974:Bromosuccinimide