[Radio-immunoassay of antidiuretic hormone. Choice of a technic of extraction, Normal and pathological values (author's transl)].
The low serum concentration of ADH requires extraction prior to assay. Various methods are compared (direct extraction by acetone, adsorption on talcum powder. Florisil, silicilic acid, Fuller's earth, QUSO, carbon-dextran, adsormone, then elution with hydrochloric acetone). The technic finally used was extraction with Florisil. The antibody used was commercially accessible. The separation of bound from free hormone required a second antibody fixed on Sephadex (DASP). The usual values found in 31 normal subjects from 22 to 89 years were: 7,4 . 4,1 pmol/l (M 2 sigma) 1 pmol/l = 1,08 pg/ml. These assays were carried out on tissue extracts prepared by mashing tumour fragments or lymph nodes removed from a patient with the Schwartz Bartter syndrome. They showed the existence in these tissues of high concentrations of ADH immunologically identical with that present in the serum of normal subjects.
D000327:Adsorption; D000328:Adult; D000368:Aged; D006801:Humans; D007177:Inappropriate ADH Syndrome / Q000378:metabolism; D008722:Methods; D008875:Middle Aged; D011863:Radioimmunoassay; D012016:Reference Values; D014667:Vasopressins / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification
D014667:Vasopressins
Dosage radio-immunologique de l'hormone antidiurétique. Valeurs usuelles et pathologiques. Choix d'une technique d'extraction.