Effects of S-methyl glutathione, S-methyl cysteine, and the concentration of oxidized glutathione on transendothelial fluid transport.
Endothelial perfusions of preswollen rabbit corneal preparations with S-methyl glutathione at levels of 2 x 10(-4)M and 10(-3)M achieve about 70% to 100%, respectively, of the stromal thinning attained with 10(-4)M oxidized glutathione (GSSG). S-methyl cysteine at 10(-4)M is without effect and, at 10(-4)M, inhibits the fluid pump. The results are interpreted to signify that thiol-disulfide exchanges with GSSG probably are not an obligatory part of the endothelial pump mechanism. Contrary to the frequently inhibitory effect of high levels of GSSG on various enzyme systems, increasing the concentration of GSSG from 10(-4)M to 10(-2)M stimulates the first hour rate of stromal thinning 33%. Neither the total amount nor the duration of thinning, however, is significantly affected.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D003315:Cornea / Q000187:drug effects / Q000378:metabolism*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004727:Endothelium / Q000187:drug effects / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000494:pharmacology; D010084:Oxidation-Reduction; D011817:Rabbits
C008425:S-methylcysteine; D005978:Glutathione; D003545:Cysteine; C017514:S-methyl glutathione