Studies on an enzyme, S-formylglutathione hydrolase, of the dissimilatory pathway of methanol in Candida boidinii.
In Candida boidinii, S-formylglutathione formed by reaction of the glutathione-dependent formaldehyde dehydrogenase is hydrolyzed to formate and glutathione by a special enzyme, S-formylglutathione hydrolase which is induced in C. boidinii along with the other enzymes of the dissimilatory pathway during growth on CH3OH. The S-formylglutathione hydrolase was purified to apparent homogeneity and a specific activity of 1390 U/mg. The molecular weight of the native enzyme was determined as 61 000 by gel filtration and 64 000 by sedimentation-diffusion equilibrium. It is composed of two nonidentical polypeptide chains of 35 000 and 25 000 daltons. The Km-value of S-formylglutathione was found to be 0.21 mM. Glutathione is a competitive inhibitor with a Ki vaue of 18.5 mM. The enzyme is very specific for S-formylglutatione, S-acetylglutathione gave 1.3%, respectively. Other glutathione derivatives of hydroxyacids tested were not split by the S-formylglutatione hydrolase.
D002175:Candida / Q000201:enzymology*; D043182:Carboxylesterase; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004396:Coloring Agents; D004790:Enzyme Induction; D005560:Formate Dehydrogenases / Q000032:analysis; D000432:Methanol / Q000378:metabolism*; D008970:Molecular Weight; D012685:Sepharose; D013869:Thiolester Hydrolases / Q000032:analysis* / Q000096:biosynthesis; D014227:Triazines
D004396:Coloring Agents; D014227:Triazines; C026268:procion green H-E4BD; D012685:Sepharose; D005560:Formate Dehydrogenases; D043182:Carboxylesterase; C020118:ESD protein, human; D013869:Thiolester Hydrolases; C020149:s-formylglutathione hydrolase; D000432:Methanol