Use of fluorescein conjugated staphylococcal protein-A as a labeling agent for porcine lymphocytes.
Staphylococcal Protein-A conjugated to fluorescein (FPA) was compared to a fluorescein conjugated sheep immunoglobulin anti-pig IgG (FSAPG) as a labeling agent for surface IgG positive (sIg+) porcine lymphoid cells. At plateau concentration of the reagents, more lymphoid cells were labeled with FPA than with FSAPG. However, on a protein concentration basis, FPA was less sensitive than FSAPG. As a control, FPA proved to be a poor labeling agent for sIg+ bovine lymphoid cells when compared to FITC conjugated rabbit IgG anti-bovine IgG (FRABG). Adsorptions of porcine lymphoid cells onto Protein-A Sepharose were performed in order to study the differential specificity of the Protein-A for sIg and for free immunoglobulins. The results of such absorptions showed that Protein-A Sepharose, whatever its affinity for Fab fragments from porcine immunoglobulins, could be used to enrich the sIg negative lymphoid cell populations in the pig.
D000818:Animals; D002417:Cattle; D005260:Female; D005452:Fluoresceins / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G; D008214:Lymphocytes / Q000276:immunology*; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell; D012685:Sepharose / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism; D012756:Sheep; D013205:Staphylococcal Protein A / Q000378:metabolism*; D013552:Swine
D005452:Fluoresceins; D007074:Immunoglobulin G; D011947:Receptors, Antigen, B-Cell; D012709:Serum Albumin; D013205:Staphylococcal Protein A; D012685:Sepharose