High-performance liquid chromatographic determination of protoporphyrin and zinc protoporphyrin in blood.
Zinc protoporphyrin and protoporphyrin free acid in blood were determined by high-performance liquid chromatography on a C18 column. Results for 63 human blood samples obtained through a lead poisoning detection program compared favorably with the widely-used ethyl acetate--acetic acid extraction determination. Blood from 16 rats which had been maintained on water heavily spiked with chloroform or bromodichloromethane and blood from a lead-poisoned cow were examined by this procedure.
D000818:Animals; D002417:Cattle; D002725:Chloroform / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D006846:Hydrocarbons, Halogenated / Q000494:pharmacology; D007855:Lead Poisoning / Q000097:blood; D011166:Porphyrins / Q000097:blood*; D011524:Protoporphyrins / Q000097:blood*; D051381:Rats; D013050:Spectrometry, Fluorescence; D022882:Trihalomethanes; D015032:Zinc / Q000097:blood*
D006846:Hydrocarbons, Halogenated; D011166:Porphyrins; D011524:Protoporphyrins; D022882:Trihalomethanes; C017803:zinc protoporphyrin; C025191:bromodichloromethane; D002725:Chloroform; D015032:Zinc