title: Effects of dihydrotestosterone and oestradiol on sexual differentiation in male rats.
abstract: Adult male rats which had been castrated at birth and treated with the non-aromatizable androgen dihydrotestosterone propionate (DHTP) showed incomplete copulatory behaviour. When tested with oestrous female rats during treatment with testosterone propionate (TP) they readily mounted these females and showed frequent penile intromissions but rarely ejaculated. In a long series of observations the proportion of ejaculating rats in tests of 30 min did not exceed 50%. Neonatally castrated rats treated with DHTP during infancy thus seemed to be capable of ejaculation in adulthood during treatment with TP, but the threshold for the occurrence of the ejaculatory reflex seemed to be higher than in normal male rats. By replacing treatment in adulthood with TP by a combined treatment with DHTP and oestradiol benzoate (OB), the frequency of ejaculation was not increased. It was concluded that the incomplete copulatory behaviour was not due to reduced efficiency of aromatization of androgen within the brain of these rats. The addition of OB to DHTP during the neonatal period of treatment enhanced the frequency of ejaculation in adulthood. The combined treatment of 0.1 mg DHTP on days 1, 3 and 5 with 0.01 mg OB on day 1 made adult copulatory behaviour during treatment with TP indistinguishable from that of rats castrated on day 10 or rats castrated at birth and treated with TP during infancy. It was concluded that the masculine organization of systems and structures involved in the display of male copulatory behaviour occurs under the influence of both non-aromatizable androgen and oestrogen, oestrogen being most likely the substance required to 'organize' the central nervous aspects of the regulation of this behaviour. The absence neonatally of non-aromatizable androgen and/or oestrogen results in specific deficiencies in adult copulatory behaviour as compared with the behaviour of normal male rats.
mesh_terms: D000818:Animals; D002369:Castration; D003307:Copulation; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004542:Ejaculation / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D008297:Male; D051381:Rats; D012733:Sex Differentiation / Q000187:drug effects*; D012726:Sexual Behavior, Animal / Q000187:drug effects*; D013739:Testosterone / Q000494:pharmacology
chemical_list: D013196:Dihydrotestosterone; D013739:Testosterone; D004958:Estradiol