[Circular dichroism study of the reaction between the protein product of bacteriophage f1 gene 5 and synthetic polynucleotides].
The complexes of gene 5 protein (phage f1) with single-stranded and double-stranded polynucleotides were investigated by circular dichroism (CD). In our experiments the concentration of the protein varied accordingly to polynucleotide. A decrease of the CD amplitude for polynucleotide-protein complexes was shown in all the regions of wavelength studied. The data indicate that the protein is bounded to the polynucleotide. This protein bounds differently to double-stranded polynucleotides containing ribo-ribo, ribo-deoxyribo and deoxyribo-deoxyribo chains. This difference is explained by differences in the form of the secondary structure of polynucleotides, containing ribo-ribo, ribo-deoxyribo and deoxyribo-deoxyribo chains.
D001435:Bacteriophages / Q000235:genetics*; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D011061:Poly A; D011071:Poly T; D011072:Poly U; D011067:Poly dA-dT; D011119:Polynucleotides; D014764:Viral Proteins / Q000235:genetics
D011119:Polynucleotides; D014764:Viral Proteins; D011061:Poly A; D011071:Poly T; D011067:Poly dA-dT; D011072:Poly U
Izuchenie vzaimodeĭstviia belka--produkta gena 5 bakteriofaga f1 s sinteticheskimi polinukleotidami metodom krugovogo dikhroizma.