title: [The kinetic determination of glucose with the glucose dehydrogenase method using the Eppendorf automatic analyzer 5040 (author's transl)].
abstract: A kinetic determination of glucose from blood, urine and spinal fluid is described with use of the new automatic analyzer ACP 5040 Eppendorf. The method uses glucose-dehydrogenase which converts glucose to gluconic acid. The NADH formed can be measured by the increase in absorbance at 334 nm. Our variation of test methodology gives good precision, accuracy and a high performance speed. There is a good correlation with the hexokinase-glucose-6-phosphate-dehydrogenase end point method.
mesh_terms: D001322:Autoanalysis / Q000295:instrumentation; D005947:Glucose / Q000032:analysis*; D005948:Glucose Dehydrogenases; D006801:Humans; D007700:Kinetics
chemical_list: D005948:Glucose Dehydrogenases; D005947:Glucose
vernacular_title: Kinetische Glucosebestimmung nach der Glucosedehydrogenase-Methode mit dem Analysenautomaten ACP 5040 (Eppendorf).