In vitro susceptibility testing of Paracoccidioides brasiliensis to sulfonamides.
A total of 60 clinical isolates of Paracoccidioides brasiliensis were tested for susceptibility to sulfadiazine and sulfadimethoxyne by the agar dilution technique. A modification of the Mueller-Hinton medium was devised which gave good growth of the yeast form. The minimum inhibitory concentrations for only 51.6% of the isolates were in the range of the recommended blood serum concentration (50 micrograms/ml). For 6 to 8% of the isolates, the minimum inhibitory concentrations were above 200 micrograms of both sulfadiazine and sulfadimethoxyne per ml. A significant decreases in susceptibility was demonstrated for one isolate obtained from a patient relapsing during sulfonamide therapy.
D003470:Culture Media; D005658:Fungi / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010228:Paracoccidioides / Q000187:drug effects*; D010229:Paracoccidioidomycosis / Q000188:drug therapy; D012008:Recurrence; D013411:Sulfadiazine / Q000097:blood / Q000494:pharmacology* / Q000627:therapeutic use; D013412:Sulfadimethoxine / Q000097:blood / Q000494:pharmacology* / Q000627:therapeutic use
D003470:Culture Media; D013411:Sulfadiazine; D013412:Sulfadimethoxine