Immunoelectron microscopy of type III collagen in normal and scleroderma skin.
Normal and scleroderma skin was studied by indirect immuno-electron microscopy with specific antibodies against Type III collagen. Fine collagen fibrils, 200-400 A in diameter were labeled with antitype III collagen antibodies. These antibodies attached to the collagen fibril forming rings with a 650 A periodicity. Fibrils with more than 600 A in diameter were only labeled with antitype I collagen antibodies. Type III collagen was found around small blood vessels, adipocytes and smooth muscle cells, thus, corresponding to the distribution of reticulin.
D000906:Antibodies / Q000276:immunology; D003094:Collagen / Q000032:analysis* / Q000276:immunology; D006801:Humans; D008854:Microscopy, Electron; D012595:Scleroderma, Systemic / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000032:analysis* / Q000648:ultrastructure
D000906:Antibodies; D003094:Collagen