The identification of microbial metabolites of sulfolithocholic acid.
Sulfolithocholate a major detoxified form of lithocholic acid, is biotransformed by human intestinal microflora to four major metabolites. Purification of crude extracts by Sephadex LH-20 chromatography gave three major radioactive peaks, after incubation studies, which were characterized by thin-layer and gas--liquid chromatography, as well as combined gas--liquid chromatography--mass spectrometry. Peak I has a polarity similar to that for 3 beta-palmitoyl-isolithocholic acid and transesterification of the metabolite showed that it was a mixture of palmitoyl, palmitoleyl, stearyl, and oleyl esters of isolithocholate. Peak II was identified as chol-3-en-24-oic acid and Peak III was a mixture of lithocholic (mainly) and isolithocholic acids. The fatty acyl derivatives of isolithocholate and delta 3-cholenate are new microbial metabolites of sulfolithocholate and represent unique classes of bile acids that should be included in routine fecal analysis of bile acids.
D002793:Cholic Acids / Q000378:metabolism; D005243:Feces / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008095:Lithocholic Acid / Q000031:analogs & derivatives* / Q000378:metabolism
D002793:Cholic Acids; C014202:sulfolithocholic acid; D008095:Lithocholic Acid; C026883:3 beta-hydroxychol-3-en-24-oic acid