The elution of IgG from subacute sclerosing panencephalitis and multiple sclerosis brains.
IgG was extracted from sections of a multiple sclerosis brain by repeated washing with phosphate-buffered saline. After this extraction, no IgG was recovered by further washing at pH 2.2. This indicates that the IgG of the plaques has no strong affinity for insoluble constituents of the brain. Section of a subacute sclerosing panencephalitis brain were used as positive controls: after repeated extraction with phosphate-buffered saline, IgG with anti-measles antibody activity was eluted at acid pH.
D000328:Adult; D000418:Albumins / Q000032:analysis; D001921:Brain / Q000276:immunology*; D002648:Child; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000276:immunology*; D013344:Subacute Sclerosing Panencephalitis / Q000276:immunology*
D000418:Albumins; D007074:Immunoglobulin G