Microbial conversion of steffimycin and steffimycin B to 10-dihydrosteffimycin and 10-dihydrosteffimycin B.
It has been shown that steffimycin (1) and steffmycin B (2) are reduced at the C-10 carbonyl by Actinoplanes utahensis, UC-5885 and Chaetomium sp., UC-4634, respectively. Using cell-free extracts of the latter organism, the optimum conversion time, pH, and enzyme concentration have been determined for the conversion of 2 to 4. The biochemical conversion of 2 has been found to be TPNH linked.
D000192:Actinomycetales / Q000378:metabolism; D018943:Anthracyclines; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D000903:Antibiotics, Antineoplastic; D001711:Biotransformation; D002474:Cell-Free System; D002597:Chaetomium / Q000378:metabolism; D005285:Fermentation; D009279:Naphthacenes / Q000378:metabolism; D010084:Oxidation-Reduction
D018943:Anthracyclines; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D009279:Naphthacenes; C009944:steffimycin; C027402:steffimycin B; C027403:10-dihydrosteffimycin; C027404:10-dihydrosteffimycin B