Determination of nanogram levels of the anticoagulant acenocoumarin in serum by high-performance liquid chromatography.
A simple, rapid, and sensitive method for the determination fo the short-acting coumarin anticoagulant acenocoumarin in human serum is described. This drug is estracted from the acidified biological matrix together with the internal standard 5-methoxypsoralen. Separation and quantitation are performed on a high-performance liquid chromatograph with a reversed-phase column and an ultraviolet detector operating at 308 nm. Accuracy and precision are good. The lowest limit of detection is 15 microgram/L, which means that acenocoumarin concentrations can be measured in serum from the subtherapeutic to the toxic range. This method can also be used for rapid measurement of warfarin serum concentrations.
D000074:Acenocoumarol / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis; D014116:Toxicology / Q000379:methods; D014859:Warfarin / Q000097:blood
D013287:Illicit Drugs; D014859:Warfarin; D000074:Acenocoumarol