title: Subcellular localization of serotonin and motilin in the human jejunum.
abstract: 1. Analytical subcellular fractionation techniques have been applied to human jejunal homogenates to study the localization of serotonin and motilin. 2. Serotonin, detected by a fluorimetric assay, showed two distinct particulate distributions in the density gradients with modal densities of 1.14 and 1.20. Motilin, whether detected by N-terminal or C-terminal specific antibodies showed similar density distributions with a modal density of 1.20, coinciding with the denser peak of serotonin. 3. These results lend support to the histochemical suggestions that motilin is located in a a serotonin-containing granule.
mesh_terms: D002500:Centrifugation, Isopycnic; D003594:Cytoplasmic Granules / Q000032:analysis; D005768:Gastrointestinal Hormones / Q000032:analysis*; D006801:Humans; D007413:Intestinal Mucosa / Q000032:analysis; D007583:Jejunum / Q000032:analysis*; D009037:Motilin / Q000032:analysis*; D012701:Serotonin / Q000032:analysis*; D013347:Subcellular Fractions / Q000032:analysis
chemical_list: D005768:Gastrointestinal Hormones; D012701:Serotonin; D009037:Motilin