title: Amino acid and glucose transport in sarcolemmal vesicles from chick embryo heart.
abstract: Purified plasma membranes from chick embryo heart are shown to retain several functional properties of intact cardiac cells. (1) Muscarinic cholinergic receptors copurify with previously used cell-surface markers, i.e., (K+-dependent) p-nitrophenylphosphatase and insulin receptors (Paris, S., Fosset, M., Samuel, D. and Ailhaud, G. (1977) J. Mol. Cell. Cardiol. 9, 161-174). (2) Neutral amino acids (L-alanine and alpha-aminoisobutyric acid) are actively transported into these osmotically active vesicles when an Na+ electrochemical gradient is imposed. The affinity and specificity for amino acids are similar to those described for intact cardiac cells. (3) D-Glucose is taken up more rapidly than L-glucose. The sterospecific transport system is saturable and Na+-independent. The Km value is close to that observed in intact cells with glucose analogues.
mesh_terms: D000409:Alanine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002642:Chick Embryo; D005947:Glucose / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism; D012508:Sarcolemma / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids; D000621:Aminoisobutyric Acids; D011976:Receptors, Muscarinic; D012964:Sodium; D005947:Glucose; D000409:Alanine