7 alpha-Dehydroxylation of cholic acid by cell extracts of Eubacterium species V.P.I. 12708.
NADH:flavin oxidoreductase and 7 alpha-dehydroxylase were induced 5- and 90-fold, respectively, by cholic acid in cultures of Eubacterium species V.P.I. 12708. Assays of 7 alpha-dehydroxylase activity in the presence of various cofactors revealed that optimal activity was obtained in the presence of NAD+ plus FADH2. The pH optima of 7 alpha-dehydroxylase activity in whole cells and cell extracts were 7.0. The similar induction pattern of these two enzymes and the apparent cofactor requirements for 7 alpha-dehydroxylation suggest a relationship between 7 alpha-dehydroxylase and NADH:flavin oxidoreductase.
D001647:Bile Acids and Salts / Q000378:metabolism; D002793:Cholic Acids / Q000378:metabolism*; D004790:Enzyme Induction; D005051:Eubacterium / Q000201:enzymology*; D038181:FMN Reductase; D005182:Flavin-Adenine Dinucleotide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006913:Hydroxysteroid Dehydrogenases; D009243:NAD / Q000494:pharmacology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010088:Oxidoreductases; D013250:Steroid Hydroxylases / Q000378:metabolism*
D001647:Bile Acids and Salts; D002793:Cholic Acids; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D010088:Oxidoreductases; D006913:Hydroxysteroid Dehydrogenases; C025376:7 alpha-hydroxysteroid dehydrogenase; D013250:Steroid Hydroxylases; D038181:FMN Reductase; D009247:NADH, NADPH Oxidoreductases