[Study of fusion of bacteriophage f2 double-stranded RNA, poly(A).poly(U), and poly(G).poly(C) in the presence of tetraethylammonium bromide].
The data on the dependence of the melting curve parameters of double-stranded RNA (replicative form of RNA of f2 bacteriophage) poly(A) times poly(U) and poly(G) times poly(C) on the concentration of (C2H5)4NBr were obtained. The RNA melting range width is shown to pass through the minimum value T =2.1+/-0.1degrees at the point of inversion of relative stability of GC and AU pairs that corresponds to 4.0+/-0.1 M concentration of (C2H5)4NBr. Using the melting temperatures of poly(A) times poly(U) and poly(G) times poly(C) the rependence of Tgc-Tau parameter on (C2H5)4NBr concentration was shown. It was concluded from these data that the effect of the double-stranded RNA stacking heterogeneity was negligible in the 0-3 M range of (C2H5)4NBr concentration. Melting curves of RNA were obtained at various values of Tgc-Tau parameter. It was shown that the profile of fine structure of melting curves depends on the value of Tgc-Tau parameter.
D001435:Bacteriophages / Q000235:genetics; D001965:Bromides / Q000494:pharmacology; D006358:Hot Temperature; D009691:Nucleic Acid Denaturation / Q000187:drug effects*; D011063:Poly A-U; D011066:Poly C; D011068:Poly G; D011131:Polyribonucleotides; D012330:RNA, Double-Stranded; D013757:Tetraethylammonium Compounds / Q000494:pharmacology*
D001965:Bromides; D011131:Polyribonucleotides; D012330:RNA, Double-Stranded; D013757:Tetraethylammonium Compounds; D011063:Poly A-U; D011068:Poly G; D011066:Poly C
Issledovanie plavleniia dvukhtsepochechnoĭ RNK bakteriofaga f2, poli (A).polu(U) and poli(G).poli (C) v prisutstvii bromistogo tetraétilammoniia.