Mercury-binding, copper-zinc proteins from rat kidney. Amino acid composition, molecular wieght and metal content.
Three isoforms of mercury-binding, copper-zinc protein were isolated from kidney of rats exposed to mercuric chloride. They contained 56-67 micrograms Hg/mg protein, 23.7-43.9 micrograms Cu/mg and 1.8-5.4 micrograms Zn/mg protein. Amino acid composition of isoforms 1, 2 and 3 was very similar, and also close to that of hepatic [Hg,Zn]- and [Cd,Zn]-metallothionein. Isoforms 1, 2 and 3 contained 29.7, 27.2 and 29.3 mol% cysteine, respectively. The molecular weight of isoforms 1, 2 and 3 was 6580, 6880 and 7000, respectively.
D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D005260:Female; D007668:Kidney / Q000378:metabolism*; D008667:Metalloproteins / Q000032:analysis*; D008970:Molecular Weight; D051381:Rats
D000596:Amino Acids; D002352:Carrier Proteins; D008667:Metalloproteins; C026931:mercury-binding copper-zinc proteins