Determination of amitriptyline-N-oxide, amitriptyline and nortriptyline in serum and plasma by high-performance liquid chromatography.
A method for the determination of amitriptyline-N-oxide, amitriptyline and nortriptyline in serum and plasma has been developed. After extraction from serum or plasma the drugs were analysed by high-performance liquid chromatography. The detection limit was 10 ng/ml (2 ml serum or plasma actually used). The coefficient of variation for all three compounds was below 10%. Amitriptyline-N-oxide was found in rat plasma after an oral dose (10 mg/kg) of amitriptyline-N-oxide.
D000639:Amitriptyline / Q000031:analogs & derivatives* / Q000097:blood*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003975:Diazepam; D008297:Male; D009661:Nortriptyline / Q000097:blood*; D051381:Rats
D000639:Amitriptyline; D009661:Nortriptyline; D003975:Diazepam; C006566:amitriptyline N-oxide