Glucaric acid excretion. Analysis of the enzymatic assay and findings in smokers and non-smokers.
Glucaric acid (GA) excretion in urine from cigarette smokers and non-smoking control subjects was determined enzymatically. There was no difference between GA 41.0 +/- 3.2 mumol/g creatinine (Cn; mean +/- SEM) in smokers and GA 43.1 +/- 3.4 mumol/g Cn in the controls. Gas chromatographic analysis of the GA-glucarolactone equilibrium was carried out in an attempt to elucidate the striking discrepancies in previous results.
D002849:Chromatography, Gas; D005260:Female; D005937:Glucaric Acid / Q000652:urine*; D005966:Glucuronidase / Q000037:antagonists & inhibitors; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D012907:Smoking; D013400:Sugar Acids / Q000652:urine*; D013696:Temperature
D013400:Sugar Acids; D005966:Glucuronidase; D005937:Glucaric Acid