[A technique of transposed dog kidney perfusion at steady pressure (author's transl)].
One limitation of the technique of isolated dog kidney perfusion with heparinized blood is the progressive impairment of the organ function which leads to perfuse separately and simultaneously the two kidneys of the same animal with two identical apparatuses, one of the kidneys being used as a control. In order to overcome this difficulty, we describe a technique of perfusion where the kidney is transposed to the femoral or the carotido-jugular vessels. Interposition of a reservoir and a ventricular pump between the vessels and the kidney secures perfusion at a steady pressure. In 10 experiments, haemodynamics and functions of the kidneys remained steady and nearly normal during a 150 min perfusion.
D000818:Animals; D004285:Dogs; D007668:Kidney / Q000098:blood supply / Q000502:physiology*; D010477:Perfusion / Q000379:methods*; D012039:Regional Blood Flow; D013997:Time Factors
Technique de perfusion à pression constante de reins transposés.