title: Human endothelial culture supernatant (HECS): a growth factor for hybridomas.
abstract: Human endothelial culture supernatant (HECS) had strong growth-promotion activity for hybridoma cells: the yield of hybridomas after fusion was increased at least 2-fold over that in the presence of feeder cells, such as mouse macrophages and spleen cells. Moreover, HECS could substitute for feeder cells when hybridomas were cultured at the single-cell level, and strongly enhanced the proliferation of hybrid cells. furthermore, the presence of human endothelial cells prolonged the stability of human-mouse hybridomas, producing human immunoglobulin, hybrids known to survive poorly during culture in vitro.
mesh_terms: D000818:Animals; D002455:Cell Division; D002459:Cell Fusion; D002470:Cell Survival; D002478:Cells, Cultured; D004727:Endothelium / Q000276:immunology; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D007136:Immunoglobulins / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D011458:Prostaglandins E / Q000494:pharmacology
chemical_list: D006133:Growth Substances; D007136:Immunoglobulins; D011458:Prostaglandins E