title: Comparison of multiple rosetting assays for detecting antibody reactivity of different immunoglobulin classes against surface antigens of benign and malignant tissue culture cells.
abstract: Four serological assays for detection of antibody reactivity against surface antigens of benign and malignant cells have been evaluated. In all four assays, antibody attached to the surface of a target cell is detected by a rosette of indicator red blood cells. Of the four assays (antibody mixed hemadsorption assay, immune adherence assay, anti-C3 mixed hemadsorption assay and protein A assay), the anti-C3 mixed hemadsorption assay showed highest sensitivity. By using purified IgM and IgG fractions we could demonstrate that, except for the protein A assay, all assays show reactivity both with IgG and IgM. Protein A assay detected only the IgG fraction, with high sensitivity. The optimization of the four assays is described and the importance of using multiple assays is emphasized.
mesh_terms: D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface; D001254:Astrocytoma / Q000276:immunology; D002478:Cells, Cultured; D003176:Complement C3 / Q000276:immunology; D006382:Hemadsorption; D006801:Humans; D007104:Immune Adherence Reaction; D007136:Immunoglobulins / Q000145:classification*; D008297:Male; D012397:Rosette Formation; D013205:Staphylococcal Protein A; D013696:Temperature
chemical_list: D000954:Antigens, Surface; D003176:Complement C3; D007136:Immunoglobulins; D013205:Staphylococcal Protein A