Purification of an enzyme with lysophospholipase activity from rat intestinal mucosa by hydrophobic chromatography.
A method based on hydrophobic chromatography is described for the purification of a lysophospholipase from the mucosa of rat small intestine. Under the conditions used, one single chromatographic run on phenyl-Sepharose CL-4B yielded a 582-fold purification with a 79% overall recovery of enzyme activity. A subsodium dodecyl sulphate gel electrophoresis; its isoelectric point is 5.6.
D000818:Animals; D002847:Chromatography, Agarose / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D007413:Intestinal Mucosa / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology*; D007526:Isoelectric Point; D008245:Lysophospholipase / Q000302:isolation & purification*; D008970:Molecular Weight; D010740:Phospholipases / Q000302:isolation & purification*; D051381:Rats
D010740:Phospholipases; D008245:Lysophospholipase