Direct derivatization and gas chromatographic determination of barbiturates in autopsy liver tissues.
A new gas chromatographic procedure is presented for the determination of barbiturates in autopsy tissues (liver and blood). The barbiturates are separated from the interference of co-extracted, free fatty acids in an acid-catalyzed methylation of the fatty acids and quantified as the N,N-dimethyl derivatives following reaction with dimethyl sulphate. Derivatization and recovery are shown to be simple, efficient procedures leading to substantially higher analytical results.
D000654:Amobarbital / Q000032:analysis; D001463:Barbiturates / Q000032:analysis* / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods*; D005230:Fatty Acids, Nonesterified; D006801:Humans; D008099:Liver / Q000032:analysis*; D010424:Pentobarbital / Q000032:analysis; D010634:Phenobarbital / Q000032:analysis
D001463:Barbiturates; D005230:Fatty Acids, Nonesterified; D000654:Amobarbital; D010424:Pentobarbital; D010634:Phenobarbital