[Certain physico-chemical properties of bacteriophage phiKZ].
Using UV-spectroscopy and circular dichroism (CD) phi KZ bacteriophage and its DNA were investigated. From the value of optical densities in the 320--400 nm range the size of the phi KZ bacteriophage's head was determined; the diameter of phi KZ bacteriophage head was found to be equal to 1300 +/- 100 A. phi KZ bacteriophage DNA has block structure and the GC-pair content is equal to 43.8 +/- 0.3%. phi KZ bacteriophage CD spectrum has an unusual profile (in comparison with known bacteriophage CD spectra); this spectrum is similar to the CD spectra of DNA in polyethylene glycole solution. To our knowledge CD spectra of such type were not obtained for other bacteriophages.
D001435:Bacteriophages / Q000648:ultrastructure; D055598:Chemical Phenomena; D002621:Chemistry; D002942:Circular Dichroism; D003596:Cytosine / Q000032:analysis; D004279:DNA, Viral; D006151:Guanosine / Q000032:analysis; D006358:Hot Temperature; D009691:Nucleic Acid Denaturation; D013056:Spectrophotometry, Ultraviolet
D004279:DNA, Viral; D006151:Guanosine; D003596:Cytosine
Nekotorye fiziko-khimicheskie svoĭstva bakteriofaga phiKZ.