Cell kinetic analysis of the quiescent cell compartment in cultured renal carcinoma of Syrian hamster.
Using a culture line of renal carcinoma of Syrian hamster (HKC-400), the distribution of DNA content in individual cells was analyzed by autoradiography after pulse and continuous labeling with 3H-thymidine in order to investigate the behavior of the non-proliferative cell compartment. Additional data used were the cell cycle parameters obtained by the fraction of labeled mitoses (FLM) method and the growth rate. The principle of analysis is to compare the cell fractions having G1 and G2 DNA contents with the fractions of G1 and G2 phase cells. It was concluded that even in the exponential phase of growth 31% of cells were blocked in either the G1 or G2 DNA region (Q2 = 22% and Q2 = 9%, and the cell loss rate from Q1 was higher than from Q2.
D000818:Animals; D001345:Autoradiography; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D002478:Cells, Cultured; D006224:Cricetinae; D004273:DNA, Neoplasm / Q000378:metabolism*; D007680:Kidney Neoplasms / Q000378:metabolism / Q000473:pathology*; D007700:Kinetics; D008214:Lymphocytes / Q000032:analysis; D008647:Mesocricetus
D004273:DNA, Neoplasm