An electron microscopic, enzyme cytochemical study on the localization of lactate dehydrogenase (LDH) in osteoclasts and peritoneal macrophages of the rat and its implication for the process of bone resorption and the origin of osteoclasts.
LDH is localized along various intracytoplasmatic membranes of osteoclasts. Macrophages show membrane-bound LDH only after phagocytosis of calcium hydroxylapatite, the main mineral constituent of bone. The localization of LDH in these macrophages is almost the same as in osteoclasts. The significance of this finding, and its possible implication in the process of bone resorption and the origin of osteoclasts are discussed.
D000818:Animals; D001862:Bone Resorption; D006651:Histocytochemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008264:Macrophages / Q000201:enzymology* / Q000276:immunology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D010010:Osteoclasts / Q000201:enzymology* / Q000648:ultrastructure; D010587:Phagocytosis; D051381:Rats
D007770:L-Lactate Dehydrogenase