title: Development of a microtitration complement-fixation test for diagnosis of Mycoplasma gallisepticum infection in chickens.
abstract: A direct microtitration complement-fixation (CF) test was developed for diagnosis of Mycoplasma gallisepticum infection in chickens. CF titers were demonstrated three weeks postinoculation in chickens inoculated intratracheally with M. gallisepticum. The direct microtitration CF test appeared comparable to the hemagglutination-inhibition test in sensitivity and specificity. Addition of fresh chicken serum to lyophilized guinea pig complement (GPC') was not necessary to enhance fixaton of GPC' by the immune complex of M. gallisepticum CF antigen and chicken MG antibodies. Attempts to use this CF test in detecting antibodies against Mycoplasma synoviae in chickens failed because the M. synoviae CF antigen was often anticomplementary and nonspecific.
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D002645:Chickens; D003168:Complement Fixation Tests; D006385:Hemagglutination Inhibition Tests; D009174:Mycoplasma / Q000276:immunology; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D011201:Poultry Diseases / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial