Development of a specific antispermine antibody.
Highly specific antibody to spermine was produced in the rabbits by immunization with a spermine-thyroglobulin conjugate of high molar ratio (740:1). Cross-reactivity with spermidine was 1.4-2.2 percent and negligible with putrescine and other compounds of similar structure. The average affinity constant of five pools of antispermine rabbit antisera was Ka = 1.8 +/- 0.2 X 10(8) liter/mole.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions; D008297:Male; D011073:Polyamines / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D013096:Spermine / Q000276:immunology*; D013954:Thyroglobulin / Q000096:biosynthesis
D000906:Antibodies; D011073:Polyamines; D013096:Spermine; D013954:Thyroglobulin