Estradiol-induced release of immunosuppressive agent from human malignant melanoma cells.
One human skin fibroblast (HSF) and three human malignant melanoma cell (HMMC) lines were examined for responsiveness to 17 alpha- and 17 beta- estradiol. Responsiveness was monitored by changes in 3H-uridine uptake (nucleic acid synthesis). 3H-leucine uptake (protein synthesis) and to complement-mediated serum cytotoxicity. 17 alpha-Estradiol had no effect on any of the examined cell lines. 17 beta-Estradiol had no effect on HSF, but increased HMMC resistance to complement-mediated serum cytotoxicity, nucleic acid and protein synthesis. An immunosuppressive agent was isolated from HMMC lysates and spent cell-free culture medium.
D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D003602:Cytotoxicity, Immunologic; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005342:Fibrinolysis; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007166:Immunosuppressive Agents; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D008545:Melanoma / Q000276:immunology*; D008934:Mitogens / Q000494:pharmacology; D011960:Receptors, Estrogen
D007106:Immune Sera; D007166:Immunosuppressive Agents; D008934:Mitogens; D011960:Receptors, Estrogen; D004958:Estradiol