A streptomycin dependent live Pasteurella multocida vaccine for the prevention of rabbit pasteurellosis.
A culture of Pasteurella multocida was isolated from a rabbit exhibiting clinical signs of respiratory pasteurellosis. Organisms from this culture were incubated in the presence of the mutagen n-methyl-n'-nitro-n-nitrosoguandine in order to obtain a streptomycin dependent mutant. A live vaccine was prepared from this mutant, and healthy pasteurella-free rabbits were vaccinated either by intranasal or subcutaneous administration. Either method provided complete protection against homologous, wild-type challenge as evidenced by absence of clinical signs or gross pathology and failure to isolate the organism from mucous secretions of tissues from vaccinated individuals.
D000818:Animals; D001428:Bacterial Vaccines; D008297:Male; D010325:Pasteurella / Q000187:drug effects / Q000276:immunology*; D010326:Pasteurella Infections / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D011817:Rabbits; D013307:Streptomycin / Q000494:pharmacology*
D001428:Bacterial Vaccines; D013307:Streptomycin