Effect of receptor occupancy on [3H]dexamethasone binding to circulating leukocytes.
To determine the effect of steroid occupancy of the glucocorticoid receptor on the measurement of total receptor concentration using Scatchard plot of [3H]dexamethasone (Dex) binding to circulating leukocytes, leukocytes were incubated with 10(-7)M cortisol or 10(-9)M Dex prior to [3H]Dex binding study. Total binding capacity calculated from Scatchard plot analysis of [3H]Dex binding was not significantly reduced after pre-incubation with cortisol. However, total binding capacity was significantly lower after pre-incubation with Dex. These data suggest that total glucocorticoid receptor concentration can be measured from a 3 h incubation of leukocytes obtained from untreated patients.
D000818:Animals; D003907:Dexamethasone / Q000097:blood*; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology; D006041:Goats; D006854:Hydrocortisone / Q000494:pharmacology; D007202:Indicators and Reagents; D007962:Leukocytes / Q000378:metabolism*; D008722:Methods; D011965:Receptors, Glucocorticoid / Q000032:analysis*; D011987:Receptors, Steroid / Q000032:analysis*
D005938:Glucocorticoids; D007202:Indicators and Reagents; D011965:Receptors, Glucocorticoid; D011987:Receptors, Steroid; D003907:Dexamethasone; D006854:Hydrocortisone