The radioimmunoassay of buprenorphine.
Antisera to buprenorphine were obtained in rabbits immunised with 3-0-carboxymethylbuprenorphine and N-hemisuccinyl-norbuprenorphine conjugated to bovine serum albumin. Using the latter antiserum and tritium labelled buprenorphine a radioimmunoassay have good accuracy and precision was developed for concentrations as low as 50 picograms in 1 ml of plasma. The N-hemisuccinyl antiserum crossreacted with norbuprenorphine, and the 3-0-glucuronide conjugate with the 3-0-carboxymethyl antiserum. Cross-reactivity of both antisera to other pharmacologically related compounds was negligible. The assay was employed to determine plasma buprenorphine concentration following its parenteral administration to dog and man.
D000818:Animals; D000918:Antibody Specificity; D002047:Buprenorphine / Q000097:blood* / Q000276:immunology; D004285:Dogs; D006801:Humans; D008297:Male; D009019:Morphinans / Q000097:blood*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods
D009019:Morphinans; D002047:Buprenorphine