[Growth in vitro of human bone marrow after freezing in liquid nitrogen: analysis of a case].
Human marrow is frozen according to the Schafer's technique; CFU-C proliferation and differentiation is then evaluated in relation to the time of storage in liquid nitrogen and to the time of in vitro culture in agar system according to the Pike and Robinson's technique. The highest plating efficiency is observed at the twelfth day of culture while in fresh cell suspension the growth peaks at the eighth day.
D001854:Bone Marrow Cells; D002455:Cell Division; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005615:Freezing; D006412:Hematopoietic Stem Cells; D006801:Humans; D066298:In Vitro Techniques; D009584:Nitrogen; D014021:Tissue Preservation
D009584:Nitrogen
La crescita in vitro del midollo osseo umano dopo congelamento in azoto liquido: analisi di un caso.