A rapid and efficient procedure for the removal of nonviable cells from suspension culture of lymphoid cell lines.
The selective removal of nonviable cells from suspension cultures of lymphoid cell lines by buoyant density centrifugation at 400 x g on a Ficoll-Hypaque (FH) cushion is described. Viable cells collect at the medium-FH interface whereas nonviable cells pellet. This method has proven useful in separating viable from nonviable cells with human lymphoblastoid cell lines, rabbit lymphoid cell lines and mouse myeloma and hybridoma cell lines.
D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D002470:Cell Survival; D006801:Humans; D008221:Lymphoid Tissue; D051379:Mice; D011817:Rabbits