title: Human melanoma tumor-specific antigens (II): Comparison of antigens from tumor cell plasma membranes of five melanoma patients.
abstract: Plasma membranes were purified from tumor tissue of five malignant melanoma patients. The purified membranes were solubilized by sonication in KC1 in the presence of Triton X 100. The soluble membranes that were obtained by this procedure contained human melanoma tumor-specific antigens. The antigens were separated from the other soluble plasma membrane proteins by chromatography on affinity columns made from the patients' tumor-specific pre-absorbed IgG coupled to Sepharose 4B. Specific antigens which were eluted from these affinity columns with potassium thiocyanate were determined by crossover immunoelectrophoresis. The five tumors appear to have a similar complement of melanoma tumor-specific plasma membrane-associated antigens.
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D006801:Humans; D007122:Immunoelectrophoresis; D008545:Melanoma / Q000276:immunology*; D008970:Molecular Weight
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface