Isolation and susceptibility to nucleases of transcriptionally active and inactive chromatin fractions from Physarum polycephalum.
Transcriptionally active and inactive chromatin fractions were isolated from Physarum polycephalum after depolymerization of chromatin with DNAase II or micrococcal nuclease, followed by fractionation in 5 mM-MgCl2. The active fraction of chromatin comprised up to 21% of nuclear DNA and was enriched 22-fold in the labelled nascent RNA. Both chromatin fractions were shown to have the nucleosomal structure. DNA of the active fraction of chromatin was degraded much faster with DNAase I and micrococcal nuclease than the DNA of the inactive fraction.
D002843:Chromatin / Q000302:isolation & purification*; D046508:Culture Techniques; D004271:DNA, Fungal / Q000302:isolation & purification; D003851:Deoxyribonucleases / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008836:Micrococcal Nuclease / Q000494:pharmacology; D010804:Physarum / Q000235:genetics*; D014158:Transcription, Genetic
D002843:Chromatin; D004271:DNA, Fungal; D003851:Deoxyribonucleases; D008836:Micrococcal Nuclease