Proteins of metaphase chromosomes and interphase chromatin.
Metaphase chromosomal and interphase chromatin proteins from cells of two species have been compared by polyacrylamide gel electrophoresis. Consistent, common changes in the quantitative distribution of the nonhistone chromosomal proteins are observed in both species. Proteins of ca. 65,000 and 68,000 MW are enriched in interphase chromatin while proteins of ca. 50,000 and 200,000 are more prominent components of metaphase chromosomes. A group of proteins of 90,000-100,000 are also increased in metaphase chromosomes compared to interphase chromatin. By two dimensional gel analysis, the most abundant proteins from chromosomes of both cell types are similar, suggesting a structural role for these nonhistone proteins (1).
D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification / Q000378:metabolism; D002875:Chromosomes / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007399:Interphase; D008677:Metaphase; D008970:Molecular Weight; D009698:Nucleoproteins / Q000302:isolation & purification / Q000378:metabolism*; D010053:Ovary
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D009698:Nucleoproteins