[Use of a modified Aldridge method for the elimination of certain amino acids and protein components interfering with the determination of thiocyanate in human and animal sera (author's transl)].
Since the amino acids and protein components remaining in the supernatant after protein precipitation react with bromine to give cyanogen bromide just as thiocyanate, they interfere with the determination of human and animal sera by the Aldridge method. This interference is widely eliminated by hydrochlorination of the amino acids or by reduction of the redox potential of bromine in an acid medium.
D000596:Amino Acids / Q000097:blood*; D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification*; D001966:Bromine; D011232:Chemical Precipitation; D006801:Humans; D006851:Hydrochloric Acid; D010084:Oxidation-Reduction; D013861:Thiocyanates / Q000097:blood*
D000596:Amino Acids; D001798:Blood Proteins; D013861:Thiocyanates; D006851:Hydrochloric Acid; D001966:Bromine
Anwendung einer modifizierten Aldridge-Methode zur Eliminierung einiger Aminosäuren und Proteinbestandteile als Störfaktoren der Thiocyanatbestimmung in menschlichen tierischen Seren.