Immunochemical characterization and serum concentrations of pregnancy-associated serum proteins in mice.
The glycoprotein structure of two pregnancy-associated murine proteins, alpha 1-PAMP and alpha 2-PAMP, was analysed by using concanavalin A (Con A) affinity electrophoresis: alpha 1-PAMP was completely precipitated by free Con A, whereas alpha 2-PAMP showed heterogeneity. The isoelectric points of alpha 1-PAMP and alpha 2-PAMP were determined at 4.2 and 4.0 respectively by using crossed immunoelectrofocusing. The alpha 2-PAMP was detectable only in the serum of pregnant mice and fetuses. Maternal serum concentrations increased from Day 8 of pregnancy, remained at high values between Days 14 and 18, and could not be detected by 2 days post partum.
D000653:Amniotic Fluid / Q000032:analysis; D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D005260:Female; D005312:Fetal Blood / Q000032:analysis; D006023:Glycoproteins / Q000097:blood; D006168:Guinea Pigs; D006801:Humans; D007122:Immunoelectrophoresis; D007526:Isoelectric Point; D051379:Mice; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood* / Q000276:immunology; D011270:Pregnancy, Animal; D051381:Rats; D013552:Swine
D006023:Glycoproteins; D011257:Pregnancy Proteins