Effect of spironolactone on androgen-dependent proteins in the ventral prostate of the rat.
The effect of spironolactone on five androgen-dependent proteins in the ventral prostate of the rat was investigated by two-dimensional gel electrophoresis. Spironolactone was given to intact male, castrated and androgen-stimulated castrated rats. It has been shown that spironolactone had no influence on the synthesis or accumulation of the androgen-dependent proteins in intact animals. However, spironolactone suppressed the restoration of the major androgen-dependent protein of low molecular weight in castrated rats given testosterone. The mechanism by which spironolactone exerts its anti-androgenic activity was shown to be unrelated to its capacity to inhibit the synthesis or accumulation of the five androgen-dependent proteins studied in this investigation.
D000818:Animals; D002369:Castration; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D013148:Spironolactone / Q000494:pharmacology*
D011506:Proteins; D013148:Spironolactone