title: Gangliotetraosyl-ceramide, the rat T lymphocyte-macrophage-associated antigen: chemical detection and cellular distribution.
abstract: The presence of gangliotetraosyl-ceramide, i.e., Gal beta 1 leads to 3GalNAc beta 1 leads to 4Gal beta 1 leads to 4Glc beta 1 leads to Cer, the T lymphocyte-macrophage-associated antigen, on lymph node cells, thymic lymphocytes, and peritoneal exudate cells of the rat was established. For this, the glycosphingo-lipids of the cells were extracted and isolated by a microseparation and detection method. In contrast to immunoglobulin-negative rat lymph node cells, immunoglobulin-bearing lymph node cells showed no detectable gangliotetraosyl-ceramide.
mesh_terms: D000818:Animals; D000941:Antigens; D001202:Ascitic Fluid / Q000166:cytology; D001345:Autoradiography; D002469:Cell Separation; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D005732:Gangliosides; D006028:Glycosphingolipids; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051381:Rats; D013950:Thymus Gland / Q000166:cytology
chemical_list: D000941:Antigens; C011258:G(A1) ganglioside; D005732:Gangliosides; D006028:Glycosphingolipids