title: Development of a canine model for long-term studies after mitral valve replacement with the hall-kaster prosthesis.
abstract: The present study was designed for long-term evaluation of the recently developed Hall-Kaster heart valve prosthesis. Mitral valve replacement was performed in 33 dogs. A detailed description of the operative procedure used is given. Extracorporeal circulation was instituted using a nonhemic priming volume resulting in an extreme hemodilution with hematocrit of about 15%. 19 of the animals died within 24 h, while 7 survived the observation period of 4--6 weeks. Thrombus on the valve was the major cause of early and late deaths. Anticoagulants were therefore given, and the therapy was made more extensive as the study progressed. The anticoagulation regime finally comprised warfarin administration preoperatively and throughout the experiment, and heparin infusion during the first 24 h postoperatively. Neomycin was used for preoperative sterilization of the gut. Later, cephalotin and penicillin were given. The use of these anticoagulants and antibiotics was found to be of major significance in providing us with a satisfactory canine model for long-term in vivo evaluation of the prosthesis.
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D006350:Heart Valve Prosthesis / Q000009:adverse effects / Q000401:mortality; D008943:Mitral Valve / Q000601:surgery*; D011183:Postoperative Complications / Q000188:drug therapy; D013927:Thrombosis / Q000209:etiology; D014859:Warfarin / Q000627:therapeutic use
chemical_list: D014859:Warfarin