Histone H1 can be removed selectively from chicken erythrocyte chromatin at near physiological conditions.
Histone H1 was depleted selectively from chicken erythrocyte polynucleosomes, without any detectable concomitant loss of H5 or core particle histones. The depletion is performed with ion exchange resin at low ionic strength (80 mM NaCl). The nucleosomes did not slide during the procedure. In contrast to the native chromatin, H1 depleted polynucleosomes are completely soluble in the 5--600 mM NaCl range.
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D006657:Histones / Q000097:blood* / Q000302:isolation & purification; D007475:Ion Exchange Resins; D009707:Nucleosomes / Q000032:analysis; D012995:Solubility
D002843:Chromatin; D006657:Histones; D007475:Ion Exchange Resins; D009707:Nucleosomes