Gas chromatographic and mass spectrometric analysis of urinary acidic metabolites of cortisol.
A method is described suitable for the analysis of the urinary acidic metabolites of cortisol which are amongst the major metabolites of this hormone (5-25% of secretion). Following hydrolysis of the urinary glucuronide conjugates and extraction of the freed steroids, methyl ester-trimethylsilyl ethers were prepared for gas chromatographic analysis. This analysis was carried out on open tubular columns coated with Carbowax stationary phase. The polar phase column permitted the complete resolution of the four acidic metabolites: alpha-cortolonic, beta-cortolonic, alpha-cortolic and beta-cortolic acids.
D002264:Carboxylic Acids / Q000652:urine; D002845:Chromatography; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006854:Hydrocortisone / Q000031:analogs & derivatives / Q000652:urine*; D013265:Stigmasterol / Q000652:urine
D002264:Carboxylic Acids; C007135:cortolonic acid; C007134:cortolic acid; D013265:Stigmasterol; D006854:Hydrocortisone