Modified hybridoma methodology: antigen-directed chemically mediated cell fusion.
The concept of antigen-directed cell fusions to increase the yield of hybridomas was investigated. To facilitate cell-cell contact, antigen conjugated cells were used in cell fusion studies. Specifically, fluorescyl conjugated murine myeloma cells (Sp 2/0-Ag14) incubated with murine immune (anti-fluorescyl) splenocytes formed aggregates containing fluorescent and non-fluorescent cells. Fusion of these populations with polyethylene glycol resulted in a greater number of anti-fluorescyl hybridomas relative to normal fusions under non-antigen directed condition. Ligand binding data indicated that despite the multi-cellular aggregates the hybridomas resulting from chemically mediated fusions produced only one monoclonal Ig.
D000818:Animals; D000941:Antigens; D001665:Binding Sites; D002417:Cattle; D002449:Cell Aggregation; D002455:Cell Division; D002459:Cell Fusion; D002999:Clone Cells / Q000276:immunology; D005452:Fluoresceins / Q000276:immunology; D006736:Horses; D006822:Hybrid Cells / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000276:immunology
D000941:Antigens; D005452:Fluoresceins