The SCE-inducing capacity of vitamin C: investigations in vitro and in vivo.
In contrast with results in vitro, vitamin C did not lead to an induction of sister-chromatid exchanges (SCEs) in the bone marrow of Chinese hamsters in the SCE test in vivo. The test dose ranged from 200 to 10 000 mg/kg body weight and was administered orally as well as by intraperitoneal injection. Sulfhydryl compounds such as cysteine and glutathione inhibited the SCE induction by vitamin C in vitro owing to their reducing capacity. These results are discussed with regard to the primary damage that may lead to an induction of SCEs by ascorbate, and the possible mutagenic danger to humans posed by vitamin C.
D000818:Animals; D001205:Ascorbic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D001853:Bone Marrow / Q000648:ultrastructure; D002460:Cell Line; D002842:Chromatids / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D003434:Crossing Over, Genetic; D004305:Dose-Response Relationship, Drug; D066298:In Vitro Techniques; D008168:Lung; D009153:Mutagens; D012854:Sister Chromatid Exchange; D013438:Sulfhydryl Compounds / Q000494:pharmacology
D009153:Mutagens; D013438:Sulfhydryl Compounds; D001205:Ascorbic Acid