Determination of the identity and organization of messenger RNAs within DNA.
We outline methods for identifying DNAs containing sequences complementary to specific mRNAs, and provide a number of complementary approaches for mapping the arrangement of mRNAs along the DNA. These methods, together with S1 nuclease mapping and the direct visualization of R-loops with the electron microscope, provide a comprehensive approach to defining the architecture of mRNAs coding for specific polypeptides and the arrangement of RNA transcripts along the genome. This detailed cartographic information can then be used to study the steps in the processing of mature mRNAs and determine the modes by which the expression of specific genes is regulated.
D004247:DNA / Q000235:genetics; D005815:Genetic Code; D006824:Hybridization, Genetic; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*
D012333:RNA, Messenger; D004247:DNA