[Study of rhodopsin sulfhydryl groups in the photoreceptor membrane].
The interaction between rhodopsin SH-groups in the photoreceptor membrane suspension with different titrating reagents (Ag HOGM, DTNB) was studied. After rhodopsin bleaching the number of free SH-groups increased from two in the dark to six after illumination. Such increase of SH-groups number was observed only by the amperometric titration procedure. The influence of the "dark" and "light exposed" SH-group modifications on regeneration ability of rhodopsin in the presence of 11-cis-retinal was investigated. No dependence of regeneration on SH-groups modification in the dark was observed, while modification of "light" SH-groups decreases rhodopsin regeneration ability. The distribution of SH-groups in rhodopsin molecules and SH-groups dependence on protein conformation are discussed.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004228:Dithionitrobenzoic Acid; D066298:In Vitro Techniques; D008027:Light; D009941:Organomercury Compounds; D010786:Photoreceptor Cells / Q000032:analysis*; D011487:Protein Conformation; D012168:Retinal Pigments; D012243:Rhodopsin / Q000528:radiation effects; D012374:Rod Cell Outer Segment / Q000032:analysis* / Q000528:radiation effects; D013438:Sulfhydryl Compounds / Q000032:analysis
D009941:Organomercury Compounds; D012168:Retinal Pigments; D013438:Sulfhydryl Compounds; C025783:ethanoxyhexamercarbide; D012243:Rhodopsin; D004228:Dithionitrobenzoic Acid
Issledovanie sul'fgidril'nykh grupp rodopsina v fotoretseptornoĭ membrane.