Stimulation of a soluble protein fraction in the hippocampus of rats, subjected to brief training.
After a brief training period (2 x 25 min) consisting of reversal of handedness, soluble protein synthesis in the hippocampus was measured by incorporation of [3H]leucine and double labelling with [3H]valine and [14C]valine together with densitometric tracings of polyacrylamide gels. Among the proteins showing increased incorporation values, 3 polypeptides of mol. wt. 13,000, 30,000 and 65,000 were found. Thus, the soluble protein-polypeptide pattern in the hippocampus was found to be altered after a short training period for the establishment of a new behavior.
D000818:Animals; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel; D007839:Functional Laterality; D006624:Hippocampus / Q000032:analysis / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats; D012193:Reversal Learning / Q000502:physiology*; D014633:Valine / Q000378:metabolism
D009419:Nerve Tissue Proteins; D007930:Leucine; D014633:Valine