Observation of a variant creatine kinase isoenzyme in sera and breast tumor cytosols.
A variant creatine kinase (CK) isoenzyme was identified in the sera of some patients who had advanced adenocarcinoma of the breast, stomach, and large intestine. A similar variant isoenzyme, together with a high concentration of CK-BB isoenzyme, was identified in some breast tumor cytosols. The variant creatine kinase activity in both sera and tumor cytosols was unaffected by antibodies specific for both the CK-M and DK-B subunits. This indicates that DK-MB isoenzyme determinations are currently best performed by electrophoretic rather than immunologic technics.
D000906:Antibodies; D001943:Breast Neoplasms / Q000201:enzymology*; D003402:Creatine Kinase / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D003600:Cytosol / Q000201:enzymology*; D004587:Electrophoresis, Agar Gel; D005260:Female; D006358:Hot Temperature; D006801:Humans; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis; D007527:Isoenzymes
D000906:Antibodies; D007074:Immunoglobulin G; D007527:Isoenzymes; D003402:Creatine Kinase