title: Uniqueness of sperm mtDNA as compared to somatic mtDNA in ram.
abstract: Sperm mtDNA exhibits unique physicochemical properties compared with mtDNA from somatic tissues in the ram. The buoyant density of sperm mtDNA was 1,6983 g/cm3 while the brain, heart and liver mtDNAs was about 1.7005 g/cm3. The Tm of the liver and sperm mtDNA was 71.0 degrees C and 69.5 degrees C, respectively. The G + C content of sperm mtDNA was 3.0 moles % lower than the liver mtDNA. The contour length of the circular sperm mtDNA was 5.01 micrometers compared with the liver mtDNA with contour length of 5.33 micrometers.
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002499:Centrifugation, Density Gradient; D004272:DNA, Mitochondrial / Q000032:analysis*; D006358:Hot Temperature; D008297:Male; D008929:Mitochondria, Heart / Q000032:analysis; D008930:Mitochondria, Liver / Q000032:analysis; D009691:Nucleic Acid Denaturation; D012756:Sheep; D013094:Spermatozoa / Q000032:analysis*
chemical_list: D004272:DNA, Mitochondrial