Cyclophosphamide-induced chromosomal aberrations in meiotic cells of male mice.
Single doses of 40, 80 and 160 mg of cyclophosphamide per kg body weight were administered intraperitoneally to C57BL/6 male mice. Cytogenetic analysis of spermatocytes at diakinesis/metaphase I, influenced as spermatogonia A and B and pachytene spermatocytes, showed the chromosomal damage. Cyclophosphamide induced structural chromosomal aberrations including translocations and interfered with the normal development of bivalents. The dose of 80 mg/kg yielded 0.6% spermatocytes with translocations and 3% translocation carriers in F1 males. Robertsonian translocations were detected in 3 males.
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology*; D005260:Female; D005298:Fertility / Q000187:drug effects; D008297:Male; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D011247:Pregnancy; D013090:Spermatocytes / Q000187:drug effects* / Q000648:ultrastructure; D013093:Spermatogonia / Q000187:drug effects / Q000648:ultrastructure; D013094:Spermatozoa / Q000187:drug effects*; D014178:Translocation, Genetic / Q000187:drug effects*
D003520:Cyclophosphamide