[Virus-specific protein synthesis in cells infected with Japanese encephalitis virus variants].
In BHK-21 and chick embryo cells infected with Japanese encephalitis virus clones, 6-7 virus-specific proteins were identified having molecular weights of 96-98, 71-74, 52-56, 22, 20, 12.5, and 10 kilodaltons (kd). After additional treatment of the infected cells with cycloheximide (300 micrograms/ml for 30 min) combined with a long-term treatment with actinomycin D (1 microgram/ml) three virus-specific proteins with the highest molecular weights were found in the cell lysate. Proteins with moleculr weights 52-56, 22, and 20 kd are glycoproteins; 14C-glucosamine in trace amounts is also incorporated into proteins with molecular weights 96-98 and 71-74 kd. Differences in the molecular weight of the intracellular protein B3 in the clones under study were found.
D000818:Animals; D002642:Chick Embryo; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004664:Encephalitis Virus, Japanese / Q000187:drug effects / Q000378:metabolism*; D014644:Genetic Variation; D008970:Molecular Weight; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*; D014776:Virus Cultivation
D014764:Viral Proteins
Sintez virusspetsificheskikh belkov v kletkakh, zarazhennykh variantami virusa iaponskogo éntsefalita.