A new method to quantitate lipid contents in human platelets by thin layer chromatography with flame ionization detection.
Lipid contents in human platelets from 21 healthy adults were analysed using thin-layer chromatography (TLC) in combination with a flame ionization detector (FID). Samples of 0.3 microliter extracted from 10(8) platelets were chromatographed on quartz rods coated with silica gel (Chromarods), and calibrated with an FID using free cholesterol and phosphatidylcholine as standards. The weight percentage of neutral lipids in human platelets obtained in this study was 14.6% which consisted mainly of free cholesterol, while that of phosphatidylethanolamine was 24.9%, phosphatidylserine plus phosphatidylinositol 6.8%, phosphatidylcholine 35.2% and sphingomyelin 18.6%. Free cholesterol in 10(8) platelets was estimated as 7 micrograms and phospholipids as 46 micrograms from standard calibrations. The minimum amount detectable by this TLC-FID method was 0.05 micrograms of standard cholesterol acetate and 0.1 microgram of lipids from 10(8) human platelets. The reproducibility was quite satisfactory and the procedure could be performed quickly and simply.
D000328:Adult; D001792:Blood Platelets / Q000032:analysis*; D002784:Cholesterol / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D005260:Female; D005410:Flame Ionization / Q000379:methods*; D006801:Humans; D008055:Lipids / Q000097:blood*; D008297:Male; D010713:Phosphatidylcholines / Q000097:blood; D010743:Phospholipids / Q000097:blood
D008055:Lipids; D010713:Phosphatidylcholines; D010743:Phospholipids; D002784:Cholesterol