Analysis of isoxazolyl penicillins and their metabolites in body fluids by high-performance liquid chromatography.
A high-performance liquid chromatographic assay method to quantitate the isoxazolyl penicillins, their active metabolites, and their penicilloic acids in serum or urine is described. Separation and analysis is performed using reversed-phase chromatography. Urine samples, after the appropriate dilution, can be assayed directly. Serum samples (0.1 ml) are either extracted with methylene chloride or treated with perchloric acid--methanol. Serum levels as low as 0.4 microgram/ml (extraction procedure) can be assayed accurately.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003023:Cloxacillin / Q000031:analogs & derivatives* / Q000097:blood* / Q000652:urine; D004009:Dicloxacillin / Q000097:blood* / Q000652:urine; D005436:Floxacillin / Q000097:blood* / Q000652:urine; D006801:Humans; D007700:Kinetics; D010068:Oxacillin / Q000097:blood* / Q000652:urine
D005436:Floxacillin; D004009:Dicloxacillin; D003023:Cloxacillin; D010068:Oxacillin