Antibody targeting of liposomes: cell specificity obtained by conjugation of F(ab')2 to vesicle surface.
A method devised for conjugating liposomes with protein resulted in the binding of up to 200 micrograms of immunoglobulin G per micromole of lipid. The coupling of antibody to human erythrocyte F(ab')2 in vesicles (140 molecules per vesicle) by this method caused a 200-fold increase in the binding of vesticles to human erythrocytes and resulted in about 80 percent of the vesicle lipid and contents being associated with cells.
D000936:Antigen-Antibody Complex; D004910:Erythrocyte Membrane / Q000276:immunology; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D066298:In Vitro Techniques; D007518:Isoantibodies; D008081:Liposomes / Q000008:administration & dosage*; D008563:Membrane Lipids
D000936:Antigen-Antibody Complex; D007140:Immunoglobulin Fab Fragments; D007518:Isoantibodies; D008081:Liposomes; D008563:Membrane Lipids