Comparison of the kinetic properties of troponin-C and dansylaziridine-labeled troponin-C1.
Comparison of the kinetic properties of troponin-C and dansylaziridine-labeled troponin-C revealed that the calcium ion binding and removal reactions with the low affinity Ca2+-binding sites (sites I and II) and the resultant local conformational change are rapid processes, and that the calcium ion binding and removal reactions with the high affinity Ca2+-binding sites (sites III and IV) are also rapid, whereas the resultant local conformational change is a slow process. It is also shown in this work that the latter processes are independent of the former processes.
D000818:Animals; D001388:Aziridines / Q000494:pharmacology*; D001389:Azirines / Q000494:pharmacology*; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D003619:Dansyl Compounds; D007700:Kinetics; D008433:Mathematics; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000201:enzymology; D011485:Protein Binding; D011487:Protein Conformation; D011817:Rabbits; D014336:Troponin / Q000378:metabolism*; D019209:Troponin C
D001388:Aziridines; D001389:Azirines; D003619:Dansyl Compounds; D009124:Muscle Proteins; D014336:Troponin; D019209:Troponin C; C017173:dansylaziridine; D002118:Calcium