title: Isolation and susceptibility to nucleases of transcriptionally active and inactive chromatin fractions from Physarum polycephalum.
abstract: Transcriptionally active and inactive chromatin fractions were isolated from Physarum polycephalum after depolymerization of chromatin with DNAase II or micrococcal nuclease, followed by fractionation in 5 mM-MgCl2. The active fraction of chromatin comprised up to 21% of nuclear DNA and was enriched 22-fold in the labelled nascent RNA. Both chromatin fractions were shown to have the nucleosomal structure. DNA of the active fraction of chromatin was degraded much faster with DNAase I and micrococcal nuclease than the DNA of the inactive fraction.
mesh_terms: D002843:Chromatin / Q000302:isolation & purification*; D046508:Culture Techniques; D004271:DNA, Fungal / Q000302:isolation & purification; D003851:Deoxyribonucleases / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D008836:Micrococcal Nuclease / Q000494:pharmacology; D010804:Physarum / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D004271:DNA, Fungal; D003851:Deoxyribonucleases; D008836:Micrococcal Nuclease