Oxygen and the photodynamic action of methylene blue on fixed eukaryotic chromosomes.
In aerobic conditions, visible light irradiation of fixed eukariotic chromosomes in dilute solutions of dyes dramatically alters chromosomal structure. Our results indirectly suggest that singlet oxygen, an electronically excited and therefore reactive form of oxygen, which is produced by visible light in the presence of methylene blue, may be responsible for chromosomal alteration.
D000818:Animals; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D004175:Diptera / Q000648:ultrastructure*; D008027:Light; D008751:Methylene Blue / Q000494:pharmacology*; D010100:Oxygen / Q000378:metabolism / Q000494:pharmacology*; D010777:Photochemistry
D010100:Oxygen; D008751:Methylene Blue