Inhibition of leukopoiesis by sera from Trypanosoma congolense infected calves: partial characterization of the inhibitory factor.
Sera collected from calves infected with Trypanosoma congolense inhibited bovine granulocyte/macrophage colony formation in vitro, but not erythroid colony formation. The inhibitor was found to be TCA precipitable in nature and on Sephadex G-200 chromatography, it was found to elute with the second major peak. Sonicated T. brucei, T. congolense, and T. theileri added directly into the cultures had no effect on granulocyte/macrophage colony formation, but induced significant enhancement of erythroid colony formation.
D000818:Animals; D001769:Blood; D001853:Bone Marrow; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood*; D002850:Chromatography, Gel; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004920:Erythropoiesis; D006410:Hematopoiesis; D007962:Leukocytes / Q000502:physiology*; D051381:Rats; D014238:Trichloroacetic Acid / Q000494:pharmacology; D014353:Trypanosomiasis, African / Q000097:blood*; D014354:Trypanosomiasis, Bovine / Q000097:blood*
D003470:Culture Media; D014238:Trichloroacetic Acid