Double-stranded DNA replication intermediates in human malignant melanoma cells.
DNA synthesis was investigated in human malignant melanoma cells. The DNA was extracted in a neutral nondenaturing buffer at 25 degrees and characterized by agarose gel electrophoresis. Radioactive DNA pulse labeled for 10 to 30 min was released from the chromosomes in the form of double-stranded fragments. The release of the fragments from the chromosomes is prevented by lysing the cells at 0 degrees instead of at 25 degrees suggesting that the release is enzymatic. The size of the fragments ranges between 4.6 and 5.6 x 10(6) daltons. After a time lag, the fragments are joined together to produce a high-molecular-weight DNA.
D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002875:Chromosomes / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D004261:DNA Replication; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008545:Melanoma / Q000378:metabolism* / Q000648:ultrastructure; D008970:Molecular Weight
D004247:DNA