Mitogenic effect of bestatin on lymphocytes.
The incorporation of 3H-thymidine into the acid insoluble fraction of spleen cells was increased intraperitoneal injection of 10 microgram bestatin/mouse. Bestatin (0.01, 0.1, 1.0 microgram/ml) which was added to mouse spleen cell culture increased 3H-thymidine incorporation into lymphocytes, but 10 microgram/ml did not. This mitogenic action of bestatin was not observed when adherent cells were removed or T cells were destroyed, suggesting that bestatin causes the proliferate T cells probably through the activation of macrophages. Bestatin given to mice did not modulate mitogenicity of lectins. Bestatin treatment of human peripheral buffy coat cells increased 3H-thymidine incorporation into lymphocytes. The addition of bestatin at a high concentration such as 100 microgram/ml to mouse spleen cell cultures exhibited a high mitogenic effect on B cells in preference to T cells but this effect was not seen with bestatin at 50 microgram/ml. In this case, the mitogenicity of Con A or LPS was expanded and antibody formation to SRBC in spleen cell cultures were also stimulated.
D000626:Aminopeptidases / Q000037:antagonists & inhibitors*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D006801:Humans; D007930:Leucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D007931:Leucyl Aminopeptidase / Q000037:antagonists & inhibitors*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D008934:Mitogens / Q000494:pharmacology
D008934:Mitogens; D000626:Aminopeptidases; D007931:Leucyl Aminopeptidase; D007930:Leucine; C012211:ubenimex