Effect of preincubation in the cold on poly(U)-dependent polyphenylalanine synthesis in a cell-free rat liver system.
Preincubation of a cell-free poly(Phe) synthesis system in the cold results in a manifold stimulation of the polyphenylalanine synthesis. This stimulatory effect can be observed only if ribosomal subunits, poly(U), Mg++ and cytosolic fraction are present in the reaction mixture during cold pretreatment. This effect is abolished if the samples are preincubated at 37 degrees C before the cold treatment probably because of an inactivation of protein factors present in the cytosolic fraction.
D000818:Animals; D002474:Cell-Free System; D003080:Cold Temperature; D003600:Cytosol / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D010452:Peptide Biosynthesis; D010455:Peptides; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism; D011072:Poly U / Q000494:pharmacology*; D011132:Polyribosomes / Q000378:metabolism; D051381:Rats; D012270:Ribosomes / Q000378:metabolism
D010455:Peptides; D011072:Poly U; C012001:polyphenylalanine; D010649:Phenylalanine; D008274:Magnesium