Serogrouping single colonies of beta-hemolytic streptococci from primary throat culture plates with nitrous acid extraction and Phadebact streptococcal reagents.
The serogrouping of isolated beta-hemolytic streptococcal colonies from throat cultures by a micronitrous acid extraction method employing Phadebact Streptotoccus Test reagents was compared with results obtained with the direct-plate Phadebact procedure and the autoclave and Streptomyces albus enzyme-lysozyme extraction methods. These data were compared with those from the Lancefield grouping obtained with a capillary precipitin test. The micronitrous acid extraction method was modified to yield an uncomplicated method that provides specific coagglutination responses from one beta-hemolytic streptococcal colony that may be collected from a primary blood agar plate either on the end of an applicator stick or from a sweep of an inoculating loop from mixed growth.
D000371:Agglutination; D006801:Humans; D007202:Indicators and Reagents; D009113:Muramidase; D009608:Nitrous Acid; D010614:Pharynx / Q000382:microbiology; D012703:Serotyping / Q000379:methods*; D013291:Streptococcus / Q000145:classification* / Q000201:enzymology
D007202:Indicators and Reagents; D009113:Muramidase; D009608:Nitrous Acid