[Controlled cell cultivation. III. The flow-diffusion chamber].
A perfuse chamber is described intended for a continuous culturing of cells and tissues, for in vitro observations and for the screening of biologically active substances. The chamber is dismountable. A ring-shaped partition of the chamber, separating the pericellular zone from the external flowing one, contains to diametrically opposite diffuse filters in the form of cylindrical stoppers made of porous glass. Depending on the angle of rotation of the diffuse filter axis with respect to the axis of pipe connections of the chamber, the wanted rate of material transfer between pericellular and flowing zones is achieved. Biological tests have shown that the survival time of isolated molluscan neurones of Limnaea stagnalis in this chamber considerably exceeds that in conventional perfusion chambers without partition. The chamber can be used in studies of embryogenesis, for incubating fertilized oocytes under conditions of controlled culturing.
D000818:Animals; D002478:Cells, Cultured / Q000166:cytology*; D003470:Culture Media; D046508:Culture Techniques / Q000295:instrumentation; D004058:Diffusion; D008974:Mollusca / Q000166:cytology; D010477:Perfusion / Q000295:instrumentation
D003470:Culture Media
Upravliaemoe kul'tirovanie kletok. III. Protochno-diffuzionnaia kamera.