5-Thio-D-glucose: hypothermic responses in mice.
Adult male mice were administered several doses of 5-thio-D-glucose (5-TG) at two environmental temperatures, 4 and 22 degrees C. Both intracerebroventricular (icv) and intraperitoneal (ip) administration of 5-TG resulted in significant (P < 0.05 to P < 0.001) decrements in rectal temperature (Tre) that were dose dependent. After 30 min, the hypothermic effects were significantly (P < 0.001, icv, 100 microgram) exacerbated by cold exposure (4 vs. 22 degrees C) and were likewise intensified significantly (P < 0.005, 45 min, fed vs. 18 h fasted) by food deprivation. These reductions in Tre were accompanied by significant (P < 0.001) increases in circulating levels of glucose. The present results indicate that 5-TG may be eliciting both central and peripheral cellular glucopenia concomitant with circulatory hyperglycemia; thus, the resultant hypothermia may be arising from competitive inhibition of glycolysis by 5-TG intermediates as well as reduced availability of tissue glucose.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001833:Body Temperature Regulation; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice
D001786:Blood Glucose; C003002:5-thio-D-glucose; D005947:Glucose