Tumor promoter TPA mimics irradiation effects on the cell cycle of HeLa cells.
When asynchronous and synchronous HeLa cells were incubated with small doses (10(-7) M) of tumor promoter 12 O-tetradecanoylphorbol-13-acetate (TPA), a variety of transient alterations in the replication cycle were detected within 24 hours by the use of independent methods. Especially, a delayed passage through the S phase and influences on the G2 phase resemble x-ray irradiation effects on cell cultures. None of these alterations was observed with the hyperplasiogenic but nonpromoting 4-O-methyl-TPA.
D002453:Cell Cycle / Q000187:drug effects* / Q000528:radiation effects; D004261:DNA Replication / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D007399:Interphase / Q000187:drug effects; D010704:Phorbols / Q000494:pharmacology*; D012854:Sister Chromatid Exchange / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014965:X-Rays
D010704:Phorbols; D013755:Tetradecanoylphorbol Acetate