A simplified procedure for the estimation of arginine in plasma and urine using arginase.
To ensure reliable arginine estimation, other compounds giving positive results with Chinard's ninhydrin reaction were removed by simple ion-exchange chromatography. Eluted arginine was converted to ornithine by arginase and estimated using Chinard's ninhydrin reaction. Plasma arginine level in adults was in the range 56--98 mumol/l, and urine arginine in the range 0.58--1.77 mg/day.
D000328:Adult; D001119:Arginase; D001120:Arginine / Q000097:blood* / Q000652:urine; D002852:Chromatography, Ion Exchange; D006801:Humans
D001120:Arginine; D001119:Arginase