Use of the protein A-gold technique for the morphological study of vascular permeability.
The protein A-gold immunocytochemical technique has been applied for the ultrastructural study of vascular permeability. A capillary preparation was perfused for 30 min with a buffer solution containing albumin. After fixation and embedding, the albumin molecules were revealed on the tissue sections using anti-albumin antiserum and the protein A-gold complex. A specific labeling was obtained over the capillary lumen, the vesicular structures of the endothelial cells, and the intercapillary space. These results allowed us to conclude that the protein A-gold technique is suitable and of general applicability for the morphological study of vascular permeability.
D000400:Air Sacs / Q000098:blood supply; D000818:Animals; D001158:Arteries / Q000378:metabolism; D004524:Eels; D006046:Gold; D006651:Histocytochemistry; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000648:ultrastructure; D010539:Permeability; D013205:Staphylococcal Protein A
D007202:Indicators and Reagents; D013205:Staphylococcal Protein A; D006046:Gold