Characterization of the cytotoxic factor produced in the spleen of dengue virus-infected mice.
Data presented in the study show that the cytotoxic factor (CF) produced in the spleen of (dengue-virus) infected mice can be purified by agarose-gel electrophoresis and Sephadex G-100 gel filtration. CF is non-dialysable, heat-labile, trypsin-sensitive, unstable at acidic and alkaline pH, a macromolecular substance which sediments on ultracentrifugation and is retained by a Millipore filter of 0.45 micron size. Its approximate molecular weight is 1.15 (+/- 0.34) x 10(5) as determined by gel filtration.
D000818:Animals; D002850:Chromatography, Gel; D003602:Cytotoxicity, Immunologic; D003715:Dengue / Q000276:immunology*; D003956:Dialysis; D004587:Electrophoresis, Agar Gel; D005374:Filtration; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D051379:Mice; D013154:Spleen / Q000276:immunology*; D014357:Trypsin; D014461:Ultracentrifugation
D014357:Trypsin