title: Enzyme histochemical studies on the conducting system of the human heart.
abstract: In this communication, the results of applying various histochemical techniques for the localization of oxidoreductases, transferases, hydrolases and isomerases in the human heart are presented. The Purkinje fibres of the atrioventricular conducting system of the human heart differ from the myocardium proper in containing a slightly higher activity of most of the glycolytic and gluconeogenetic enzymes investigated. The relatively higher activity of 6-phosphofructokinase, the key enzyme in anaerobic carbohydrate metabolism, is especially noteworthy. On the other hand, the activities of some of the enzymes that play a part in the aerobic energy metabolism is slightly less than those in the myocardium fibres. As for the activity of the NADPH regenerating enzymes, the activity of 6-phosphogluconate dehydrogenase and malate dehydrogenase (oxaloacetate-decarboxylating) is somewhat higher, and the activity of glucose-6-phosphate dehydrogenase similar, in the Purkinje fibres compared to that in the myocardial fibres. The activity of myosin ATPase is similar for both types of fibre. Likewise, the fibres of the conducting system and of the myocardium show a similar activity of acid phosphatase, beta-glucuronidase, non-specific naphthylesterase and peroxidase. The neurogenic function of the conducting system of the human heart was demonstrated by the high activity of acetylcholinesterase in the Purkinje fibres and in the atrioventricular node. All these histochemical findings in Purkinje fibres are similar at widely differing levels of the conducting system.
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism; D004734:Energy Metabolism; D005943:Gluconeogenesis; D006019:Glycolysis; D006329:Heart Conduction System / Q000201:enzymology*; D006651:Histocytochemistry; D006801:Humans; D009206:Myocardium / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism; D011690:Purkinje Fibers / Q000201:enzymology*
chemical_list: D010088:Oxidoreductases; D000110:Acetylcholinesterase