Rapid analysis of naphthalene and its metabolites in biological systems: determination by high-performance liquid chromatography/fluorescence detection and by plasma desorption/chemical ionization mass spectrometry.
A rapid procedure for the determination of naphthalene and its metabolites in bile of rainbow trout and mice is described. The integrated analytical techniques combine high-performance liquid chromatography/ultraviolet fluorescence detection and plasma desorption/chemical ionization mass spectrometry for identification and quantitation. After separation by reverse-phase liquid chromatography, naphthalene and its metabolites are detected and quantitated by ultraviolet fluorescence spectrometry. Identification of two metabolites is confirmed by mass spectrometry. A direct insertion probe tip for a conventional chemical ionization mass spectrometer was modified to obtain spectra of thermally labile compounds. A spectrum of less than 100 ng of naphthyl glucuronide, a labile glucuronic acid conjugate of 1-naphthol, was obtained with this system.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008832:Microchemistry; D009281:Naphthalenes / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods; D014337:Trout
D009281:Naphthalenes