The effect of mouse serum on lipid metabolism in embryonic chick limb-bud cells.
Dissociated embryonic chick limb cells will undergo terminal differentiation in culture. The addition of whole or delipidated mouse serum to the cultures will, however, inhibit the chondrogenic potential of the cells. The inhibitory serum readily stimulates increased incorporation of arachidonic acid and palmitic acid into triacylglycerol in the treated cells, while the incorporation of arachidonate into various phospholipids is significantly lowered. In contrast mouse serum has no effect on the incorporation of inorganic [32P]phosphate into phospholipid. We interpret the patterns of incorporation of these lipid substrates as indicating that mouse serum modulates the deacylation-reacylation cycle of phospholipids (Lands' cycle), which is primarily responsible for the incorporation of arachidonic acid. This finding suggests that there may be a decrease in membrane fluidity which might play a key role in cellular regulation and differentiation.
D000818:Animals; D001769:Blood; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D005121:Extremities / Q000196:embryology; D050356:Lipid Metabolism; D008560:Membrane Fluidity; D051379:Mice; D010743:Phospholipids / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis*
D003470:Culture Media; D010743:Phospholipids; D014280:Triglycerides