Radioimmunoassay of three oestrogens and three androgens in the same plasma sample after extraction and chromatographic separation.
The plasma levels of oestrone (Oe1), 17 beta-oestradiol (Oe2), oestriol (Oe3), testosterone (T), 5 alpha-dehydrotestosterone (DHT) and androstenedione (A) were assayed by RIA in plasma obtained from peripheral venous blood. The hormones are isolated from the plasma extract, first by Sephadex LH-20 column chromatography (Oe2/Oe3/Oe1, T, DHT, A) and after Oe1, T, DHT, A by TLC on silica gel 60 F254. The accuracy, reproducibility and sensitivity of the method make it satisfactory for clinical studies.
D000728:Androgens / Q000097:blood*; D000735:Androstenedione / Q000097:blood; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D013196:Dihydrotestosterone / Q000097:blood; D004958:Estradiol / Q000097:blood; D004964:Estriol / Q000097:blood; D004967:Estrogens / Q000097:blood*; D004970:Estrone / Q000097:blood; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*; D013739:Testosterone / Q000097:blood
D000728:Androgens; D004967:Estrogens; D013196:Dihydrotestosterone; D004970:Estrone; D013739:Testosterone; D000735:Androstenedione; D004958:Estradiol; D004964:Estriol