Long-term tissue culture of human pheochromocytomas.
Two human pheochromocytomas were cultured for long periods. One was cultured for a year, the other is still growing after a cultivation time of seven months. Though decreasing with the age of cultures the cells contained catecholamines all the time. Catecholamines were demonstrated by electron microscope, by fluorescence histochemistry and by Vulpian's and Da Prada's methods. Both pheochromocytomas were responsive to Nerve Growth Factor and showed dose-dependent development of neurite-like processes.
D000310:Adrenal Gland Neoplasms / Q000473:pathology*; D000328:Adult; D002395:Catecholamines / Q000378:metabolism; D002469:Cell Separation; D046508:Culture Techniques; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008297:Male; D009414:Nerve Growth Factors / Q000494:pharmacology; D010673:Pheochromocytoma / Q000473:pathology*
D002395:Catecholamines; D009414:Nerve Growth Factors