Embryonated eggs compared with fragments of chorioallantois attached to egg shell for isolation of Newcastle disease virus.
The feasibility of using fragments of chorioallantois attached to egg shell (C-S) as an alternative to embryonated eggs for isolating Newcastle disease virus (NDV) was assessed. In paired titrations of 6 strains of virus, embryonated eggs detected an average of 6-to-7-fold greater amounts of virus than did C-S fragments. The efficiency of isolation of viscerotropic velogenic NDV from 240 swab samples was 97% in embryonated eggs and 84% in C-S fragments. Sensitivity differences between the 2 host systems were revealed only in tests of samples expected to contain small amounts of virus. These results suggest that C-S fragments can be reliably used in some but not all instances, thereby relieving logistic constraints sometimes associated with the use of embryonated eggs.
D000482:Allantois; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D002823:Chorion; D046508:Culture Techniques; D004528:Egg Shell; D005321:Extraembryonic Membranes; D009521:Newcastle Disease / Q000382:microbiology; D009522:Newcastle disease virus / Q000254:growth & development / Q000302:isolation & purification*