title: Complete amino acid sequence of the major component myoglobin from the goose-beaked whale, Ziphius cavirostris.
abstract: The complete primary structure of the major component myoglobin from the goose-beaked whale, Ziphius cavirostris, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Over 80% of the amino acid sequence was established from the three peptides resulting from the cleavage of the apomyoglobin at its two methionine residues with cyanogen bromide along with the four peptides resulting from the cleavage with trypsin of the citraconylated apomyoglobin at its three arginine residues. Further digestion of the central cyanogen bromide peptide with S. aureus strain V8 protease and the 1,2-cyclohexanedione-treated central cyanogen bromide peptide with trypsin enabled the determination of the remainder of the covalent structure. This myoglobin differs from the cetacean myoglobins determined to date at 12 to 17 positions. These large sequence differences reflect the distant taxonomic relationships between the goose-beaked whale and the other species of Cetacea the myoglobin sequences of which have previously been determined.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002591:Cetacea; D003488:Cyanogen Bromide; D009211:Myoglobin; D010455:Peptides / Q000302:isolation & purification; D013045:Species Specificity; D014357:Trypsin; D014907:Whales
chemical_list: D009211:Myoglobin; D010455:Peptides; D014357:Trypsin; D003488:Cyanogen Bromide