Separation of normal mature bone marrow plasma cells.
A three-phase purification process for the separation of normal bone marrow plasma cells is described. Material was obtained from haematologically normal humans and baboons. Known monoclonal and polyclonal B cell activators were avoided both in vivo and in vitro. In Phase I, bone marrow fragments were separated from cell suspensions by buoyant density methods. Marrow fragments were shown to be richer in plasma cells than the corresponding marrow cell suspensions. Phase II consisted of culturing fragments by a simple suspension method in which a selective affinity of plasma cells and marrow stromal cells resulted in further concentration of plasma cells with discharge of haemopoietic elements. Maximum concentration of plasma cells occurred within 7 d. In phase III, fragments were disaggregated with trypsin, and the marrow stromal cells were removed by their adherence properties. The resulting non-adherent fraction comprised approximately 85% plasma cells. The process allows for direct quantitative evaluation of normal plasma cell physiology in vitro.
D000818:Animals; D001772:Blood Cell Count; D001853:Bone Marrow / Q000032:analysis; D001854:Bone Marrow Cells; D002448:Cell Adhesion; D002449:Cell Aggregation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D010215:Papio; D010950:Plasma Cells / Q000187:drug effects; D011691:Puromycin / Q000494:pharmacology
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011691:Puromycin