title: Evidence that human growth hormone is a potent lactogen in primates.
abstract: To help determine if human GH (hGH) plays a physiological role in normal mammary function or diseases of the human breast, a study was undertaken to examine the lactogenic potency of hGH in a subhuman primate system. hGH was previously considered a relatively weak lactogen when tested in avian crop sac and rodent mammary assays. The effect of hGH on the production of alpha-lactalbumin in organ cultures of primate mammary tissue was compared with the effect of equal concentrations of ovine PRL (oPRL). Mammary tissues from seven adult rhesus monkeys were maintained in culture for 9 days (medium changed every 3 days) without and with oPRL or hGH in doses ranging from 30-1000 ng/ml. hGH was consistently more effective than oPRL in the stimulation of alpha-lactalbumin production in over 90% of the cases. For example, mean (+/- SEM) alpha-lactalbumin production at the 6-day time period in response to 1000 ng/ml hormone was 12.9 +/- 3.3 ng/ml for oPRL and 61.9 +/- 18 ng/ml for hGH. Overall, it was calculated that hGH had 328% the potency of oPRL in adult rhesus monkey mammary tissue. In pigtail macaques, hGH was also a more potent stimulant of alpha-lactalbumin production than oPRL, but the differences wer not as great as in rhesus monkeys. alpha-Lactalbumin in medium was higher in hGH-containing dishes than in those containing oPRL in over 85% of the cases. It was calculated that the lactogenic potency of hGH in macaque tissue was 160.5% that of oPRL. The differences between species were significant. These results indicate that hGH is a more effective lactogen in subhuman primates than oPRL and suggest the possibility of GH may be a lactogen which has physiological importance.
mesh_terms: D000818:Animals; D005260:Female; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D007768:Lactalbumin / Q000096:biosynthesis; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008254:Macaca nemestrina; D008297:Male; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism*; D010215:Papio; D011388:Prolactin / Q000494:pharmacology*; D013045:Species Specificity
chemical_list: D011388:Prolactin; D013006:Growth Hormone; D007768:Lactalbumin