title: Specific techniques for the identification of O-acylated sialic acids in colonic mucins.
abstract: The development of methodology for the histochemistry of mucins based upon their PAS reactivity is discussed in terms of mechanism, specificity and application. Two new histochemical methods (PB/KOH/PAS and PAT/KOH/PAS), supplemented by a variety of new and standard histochemical techniques, and correlated by parallel chemical studies, were used to demonstrate and identify C4 and side chain O-acylated sialic acids in colonic epithelial mucins. The application of these methods in the field of histopathology is discussed.
mesh_terms: D000818:Animals; D002417:Cattle; D003108:Colonic Diseases / Q000503:physiopathology; D003110:Colonic Neoplasms / Q000503:physiopathology; D006651:Histocytochemistry; D006801:Humans; D009077:Mucins / Q000032:analysis*; D010503:Periodic Acid-Schiff Reaction; D051381:Rats; D012794:Sialic Acids / Q000032:analysis*
chemical_list: D009077:Mucins; D012794:Sialic Acids