Biosynthesis of vasopressin, oxytocin, and neurophysins: isolation and characterization of two common precursors (propressophysin and prooxyphysin).
[35S]Cysteine-labeled putative precursors for vasopressin-associated neurophysin (NP-VP) and oxytocin-associated neurophysin (NP-OT) were isolated from the supraoptic nuclei (SONs) of normal rats. Homozygous Brattleboro rats were deficient in one of these precursors, the NP-VP precursor. Direct support for the hypothesis that OT and its NP- and VP and its NP are synthesized from two separate macromolecular common precursors was obtained by limited proteolysis of the precursors with trypsin and identification of the fragments as NPs, VP, or OT by a number of criteria (14). In this paper, these common precursors designated propressophysin (precursor for NP-VP and arginine VP) and prooxyphysin (precursor for NP-OT and OT) were further characterized. Propressophysin was specifically bound by Concanavalin-A-Sepharose and was eluted by 0.2 M alpha-methyl mannoside, showing that it is glycosylated. In contrast, prooxyphysin does not appear to be glycosylated. Using [3H]fucose as label injected near the SONs, two proteins (mol wt, approximately 10,000) were identified, which appear to be derived from propressophysin, that are rapidly transported to the posterior pituitary of normal rats. These two proteins were absent in homozygous Brattleboro animals. Both propressophysin and prooxyphysin were bound by a NP-Sepharose affinity support. The binding was different from that of arginine VP or OT to NP, since it was independent of pH and was hydrophobic in nature. In addition to prooxyphysin, the SONs of homozygous Brattleboro rats also contained other [35S]cysteine-labeled protein (mol wt, 20,000) composed of a NP-like protein and NP-binding peptides. The tryptic peptides derived from these proteins were very different in their chromatographic (high performance liquid chromatography) properties than the tryptic peptides derived from propressophysin and prooxyphysin. This protein ('X') was not bound by a Concanavalin-A-Sepharose affinity column, although it had chromatographic properties similar to propressophysin on Sephadex G-75.
D000818:Animals; D001127:Arginine Vasopressin; D005260:Female; D005643:Fucose / Q000378:metabolism; D006720:Homozygote; D009481:Neurophysins / Q000096:biosynthesis*; D010121:Oxytocin / Q000096:biosynthesis*; D010446:Peptide Fragments / Q000032:analysis; D010904:Pituitary Gland, Posterior / Q000378:metabolism*; D011498:Protein Precursors / Q000096:biosynthesis*; D051381:Rats; D013045:Species Specificity; D013495:Supraoptic Nucleus / Q000378:metabolism; D014357:Trypsin; D014667:Vasopressins / Q000096:biosynthesis*
D009481:Neurophysins; D010446:Peptide Fragments; D011498:Protein Precursors; D014667:Vasopressins; D001127:Arginine Vasopressin; D005643:Fucose; D010121:Oxytocin; D014357:Trypsin