Peritoneal exudate mononuclear phagocyte colony-forming cells: their proliferative state in vivo and sensitivity to cytotoxic agents.
We investigated the proliferative state of peritoneal exudate macrophage colony-forming cells (PE-CFC) by exposing them to [3H]thymidine with a high specific activity in vitro and by administering cytosine arabinoside and methotrexate in vivo. Since [3H]TdR had no appreciable killing effect on PE-CFC and since the drug treatment caused no reduction in the appearance of PE-CFC, we concluded that the majority of PE-CFC, if not all, are not in active cell cycle. The production of PE-CFC was, however, suppressed by the administration of nitrogen mustard, cyclophosphamide, BCNU and vinlastine.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D001345:Autoradiography; D002330:Carmustine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D003520:Cyclophosphamide / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008264:Macrophages / Q000166:cytology*; D008297:Male; D008466:Mechlorethamine / Q000494:pharmacology; D008727:Methotrexate / Q000494:pharmacology*; D051379:Mice; D008809:Mice, Inbred C3H / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014747:Vinblastine / Q000494:pharmacology
D003561:Cytarabine; D014316:Tritium; D008466:Mechlorethamine; D014747:Vinblastine; D003520:Cyclophosphamide; D002330:Carmustine; D013936:Thymidine; D008727:Methotrexate