Channel catfish virus: comparative replication and sensitivity of cell lines from channel catfish ovary and the brown bullhead.
A cell line derived from channel catfish ovary tissue was compared with the brown bullhead (BB) cell line for their respective abilities to replicate and detect channel catfish virus (CCV). The channel catfish ovary cell line (CCO) produced cytopathic effects (CPE) more rapidly and detected CCV at higher dilutions than did the BB cell line. Production of CCV was more rapid in CCO cells than in BB cells, but the peak titers of the two lines were not significantly different. The CCO cell line was shown to be the more sensitive cell line for CCV research and diagnostics.
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D005399:Fishes; D006564:Herpesviridae / Q000302:isolation & purification*; D010053:Ovary / Q000276:immunology