Turbidity as a cause of error in the L-alanine transferase (ALAT) method.
When performing the ALAT-determination as recommended by the Scandinavian Committee on Enzymes, turbidity will develop due to a low ionic strength of the buffer. At the end of the pre-incubation period, the turbidity may still be developing, increasing the absorbance and lowering the ALAT-activity by 0-5 U/l. Turbidity and the error introduced in the assay, can be eliminated by increasing the ionic strength of the buffer by addition of NaCl.
D000042:Absorption; D000410:Alanine Transaminase / Q000097:blood*; D011232:Chemical Precipitation; D003951:Diagnostic Errors; D006801:Humans; D007202:Indicators and Reagents; D009391:Nephelometry and Turbidimetry; D009994:Osmolar Concentration; D010783:Photometry
D007202:Indicators and Reagents; D000410:Alanine Transaminase