Inhibition of dengue virus replication by amantadine hydrochloride.
The effect of amantadine hydrochloride (1-adamantanamine hydrochloride) on dengue virus replication was examined in vitro. Amantadine decreased the titers of all four types of dengue viruses grown in LLC-MK2 cells by greater than 90% at concentrations of 50 micrograms/ml. There was no evidence for any cytopathic effect of the drug at concentrations less than 100 micrograms/ml. Studies of the time of addition showed that the antiviral effect was maximal when drug was added to virus cultures immediately after the viral adsorption period. In addition, amantadine caused a marked reduction in the growth of dengue virus type 2 in both human and rhesus peripheral blood leukocytes without affecting cell viabili ty. These findings demonstrate that amantadine significantly inhibits the replication of dengue viruses in vitro and indicate a need to determine the efficacy of this drug against dengue virus infections in vivo.
D000547:Amantadine / Q000494:pharmacology*; D000818:Animals; D003715:Dengue / Q000209:etiology; D003716:Dengue Virus / Q000187:drug effects* / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008253:Macaca mulatta; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects*
D000547:Amantadine