Isolation of subclasses of human IgG with affinity chromatography.
A method is described for the isolation of subclasses of human IgG. Two myeloma proteins, after isolation by DEAE-Sephadex chromatography, were further purified on subclass-specific anti-IgG1 and 2 Sepharose 4B columns. Contaminating IgG1 and 2 were removed from the isolated myeloma proteins.
D000818:Animals; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D006168:Guinea Pigs; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification / Q000302:isolation & purification*; D009194:Myeloma Proteins / Q000276:immunology; D011817:Rabbits
D007074:Immunoglobulin G; D009194:Myeloma Proteins