Observation of unstained biological macromolecules with the STEM.
We discuss the observation of an unstained single biological macromoilecule of human serum low density lipoprotein in a scanning transmission electron microscope (STEM). Using a carbon substrate with 16 A average thickness and an electron dose of 19 electrons/A2 the image obtained from the unstained molecule shows a similar surface substructure as that seen in a negatively stained conventional transmission electron microscope (CTEM) micrograph.
D005612:Freeze Drying / Q000379:methods; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011487:Protein Conformation / Q000528:radiation effects*
D008077:Lipoproteins, LDL