Incidence of polyene-resistant yeasts recovered from clinical specimens.
The development of resistance to amphotericin B and nystatin in yeast isolates was determined. Organisms recovered from patients on the oncology service, undergoing extensive chemotherapy for acute leukemia and bone marrow transplantation, were compared with yeasts recovered from patients on other services in the same hospital over a 7-month period. An agar dilution method was used to assay the susceptibility for each antibiotic; resistance was defined as a minimal inhibitory concentration of greater than or equal to 2 micrograms/ml for amphotericin B and greater than or equal to 16 micrograms/ml for nystatin. None of 625 isolates from 238 patients on non-oncology services demonstrated polyene resistance. Resistance only occurred in a subpopulation of oncology patients, in which 55 isolates (7.4%) from six patients (8.6%) exhibited polyene resistance. Resistance yeasts included Candida albicans (three strains), Candida tropicalis (one strain), and Torulopsis glabrata (two strains). All of the patients from whom resistant yeasts were recovered had experienced extensive chemotherapy with cytotoxic agents, granulocytopenia, and long-term treatment with both antibacterial and polyene antibiotics. Resistance to 2 micrograms of amphotericin B per ml and to 16 micrograms of nystatin per ml was associated with loss or marked depression of ergosterol in the cell membrane as measured by ultraviolet spectra. A significant incidence of polyene resistance in an oncology subpopulation was documented, suggesting a need for susceptibility testing in patients who are at high risk for development of drug-resistant fungal pathogens.
D000666:Amphotericin B / Q000494:pharmacology*; D000741:Anemia, Aplastic / Q000188:drug therapy; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004875:Ergosterol / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007938:Leukemia / Q000188:drug therapy; D009181:Mycoses / Q000382:microbiology; D011090:Polyenes / Q000494:pharmacology; D015003:Yeasts / Q000032:analysis / Q000187:drug effects* / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D011090:Polyenes; D000666:Amphotericin B; D004875:Ergosterol