Development of a specific extracted radioimmunoassay for methionine enkephalin in human plasma and cerebrospinal fluid.
A sensitive extracted radioimmunoassay for methionine enkephalin (met-enkephalin) has been developed which allows measurement of its concentration in human plasma and cerebrospinal fluid (CSF). The first evidence for the existence of met-enkephalin in the circulation of man is described and its presence in CSF confirmed. The susceptibility of the methionine residue in met-enkephalin to undergo oxidation to the methionine sulphoxide analogue was utilized. All extracted samples were oxidized by hydrogen peroxide before assay and this allowed measurement of total met-enkephalin. The assay had unique specificity with no cross-reaction with leucine enkephalin, purified human beta-endorphin or beta-lipotrophin (beta-LPH). A purification method for radio-iodinated enkephalin has been developed with octadecasilyl-silica (ODS-silica) (10 micrometers) yielding a high-affinity mono-iodinated tracer stable on storage for up to 3 months at 4 degrees C. A method to extract met-enkephalin from acidified plasma or CSF has been developed with larger particle size ODS-silica (35-70 micrometers) suitable for extracting repeated samples. Met-enkephalin immunoreactivity was detectable in plasma of all subjects tested and ranged from 14 to 140 pg/ml. In CSF, however, the range was 5-29 pg/ml. Met-enkephalin immunoreactivity was not generated by incubating exogenous or endogenous beta-LPH and beta-endorphin in plasma or CSF. Two hypopituitary subjects and one dexamethasone-suppressed subject, all with undetectable immunoreactive plasma adrenocorticotrophic hormone and NH2- and CO2H-terminal beta-LPH, had measurable met-enkephalin in their plasma suggesting met-enkephalin was not of pituitary origin nor a breakdown product of secreted beta-LPH or beta-endorphin.
D004723:Endorphins / Q000097:blood*; D004745:Enkephalins / Q000097:blood* / Q000134:cerebrospinal fluid; D006801:Humans; D008715:Methionine; D011863:Radioimmunoassay / Q000379:methods
D004723:Endorphins; D004745:Enkephalins; D008715:Methionine