Ca2+-calmodulin-dependent phosphorylation and platelet secretion.
Protein phosphorylation may play a critical role in stimulus-coupled secretion of platelets. Some platelet proteins become phosphorylated on exposure to agents such as thrombin and collagen, and the smallest of these phosphoproteins (molecular weight 20,000), has been identified as a light chain of myosin. Phosphorylation of myosin light chain increases the activity of actin-activated myosin ATPase and the resultant contraction of the actomyosin presumably mediates the release reaction. Platelet myosin light chain kinase has been identified as a calcium-dependent enzyme requiring calmodulin for its activity. Calmodulin is a Ca2+-binding protein with a molecular weight of approximately 18,000 which seems to be involved in a wide variety of cellular processes. Although a growing body of evidence suggests that non-muscle actomyosin, such as that isolated from platelets, is regulated by Ca2+-calmodulin-dependent light chain phosphorylation, the precise relationship between the phosphorylation and the function of platelets is not clearly established. We now present pharmacological evidence that a calmodulin-mediated system, such as Ca2+-dependent myosin light chain phosphorylation, also plays an important role in the phenomenon of the release reaction. N-(6-aminohexyl)-5-chloro-1-napthalene-sulphonamide (W-7) (refs 13-15) is shown to bind selectively to calmodulin in vitro and inhibit its biological activity.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000097:blood*; D002135:Calcium-Binding Proteins / Q000097:blood*; D002147:Calmodulin / Q000037:antagonists & inhibitors / Q000097:blood*; D003959:Diamines / Q000494:pharmacology; D006801:Humans; D009218:Myosins / Q000378:metabolism; D010766:Phosphorylation; D010974:Platelet Aggregation / Q000187:drug effects; D011494:Protein Kinases / Q000097:blood*; D012701:Serotonin / Q000378:metabolism; D013449:Sulfonamides / Q000494:pharmacology
D002135:Calcium-Binding Proteins; D002147:Calmodulin; D003959:Diamines; D013449:Sulfonamides; D012701:Serotonin; C017967:W 7; D000255:Adenosine Triphosphate; D011494:Protein Kinases; D009218:Myosins; D002118:Calcium