title: [Karyotype in GPG bands: description of a elementaly technique for routine (author's transl)].
abstract: A new technic for banded chromosomes, with similar results (100%) to those obtained with chromatids modification by trypsin (G bands), is described. The key consists in Pyronin addition (20 mg.%) to distilled water at hypotonic shock, following then a standard karyotype technic assayed with micro-method, reaching banding after Giemsa tinction at 3% in 6.7 phosphate pH. Its interest radicates in chromosome modification in vivo, simplicity, fiability (80% of excellent results) and reproductibility, allowing any cytogenetic laboratory a routine obtention of G bands in only four days.
mesh_terms: D001399:Azure Stains; D002871:Chromosome Banding / Q000379:methods*; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D011754:Pyronine; D014966:Xanthenes
chemical_list: D001399:Azure Stains; D014966:Xanthenes; D011754:Pyronine
vernacular_title: Cariotipo en bandas GPG. Descripción de una técnica elemental para rutina.