Effects of long contact times of misonidazole on attached Chinese hamster V79 cells.
The 2-nitroimidazole misonidazole, an hypoxic cell radiation sensitizer, has been shown to be cytotoxic to attached oxic Chinese hamster V79 cells after high doses and long contact times. This killing effect is enhanced if BrdU is incorporated into chromosomes. It was found that misonidazole slows cell progression through the cell cycle and that those cells that move into mitosis carry very few chromosomal aberrations suggesting that the majority of cells are dying in interphase. The level of induced SCEs both in 2nd and 3rd division cells was comparatively slight; being always less than twice the control level although there was an indication of an increased incidence with dose. Since human exposure to misonidazole will only be at low concentrations, these results imply that misonidazole is a comparatively safe agent, particularly when its effect are contrasted with those of other chemotherapeutic agents.
D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002460:Cell Line; D002842:Chromatids / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D003434:Crossing Over, Genetic; D004305:Dose-Response Relationship, Drug; D008168:Lung; D008920:Misonidazole / Q000494:pharmacology*; D009593:Nitroimidazoles / Q000494:pharmacology*; D012854:Sister Chromatid Exchange; D013997:Time Factors
D009593:Nitroimidazoles; D008920:Misonidazole