[DNA synthesis in isolated nuclei of Misgurnus fossilis loach embryos. I. Characteristics of the system].
System for studying DNA synthesis in the isolated nuclei of loach Misgurnus fossilis embryo was described. Nuclei from 13--15 h embryo incorporated 2--3 pmoles of [3H]TMP or [3H]dCMP per microgram of DNA during 30 min of incubation in the reaction mixture. The incorporation was inhibited by ethidium bromide, actinomycin D, and N-ethylmaleimide. The incorporation was Mg2+- and ATP-dependent. Adenosine diphosphate at a concentration of 10 mM enhanced the label incorporation into DNA about twofold. Nicotinamide adenine dinucleotide, polyamines, heparin, and histones inhibited label incorporation into nuclear DNA.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D003843:Deoxycytidine Monophosphate / Q000378:metabolism; D005399:Fishes / Q000196:embryology*; D006493:Heparin / Q000494:pharmacology; D006657:Histones / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D013942:Thymine Nucleotides / Q000378:metabolism
D006657:Histones; D013942:Thymine Nucleotides; D003843:Deoxycytidine Monophosphate; D012965:Sodium Chloride; D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D006493:Heparin; D004247:DNA; D008274:Magnesium
Sintez DNK v izolirovannykh iadrakh zarodysheĭ v'iuna Misgurnus fossilis. I. Kharakteristika sistemy.