[Cardioplegic haemoglobin-perfusion for myocardial protection. Experiments on isolated rat-hearts (author's transl)].
On isolated normothermic and constant-pressure perfused rat-hearts cardioplegic haemoglobin-perfusion was compared with conventional cardioplegic perfusion without haemoglobin. The addition of 4% haemoglobin to the cardioplegic solution guarantees oxygen demand of the pharmacologically arrested heart at physiological PO2 (about 100 mm Hg) of the perfusion-solution, while conventional cardioplegic perfusion without haemoglobin needs arterial PO2 > 600 mm Hg and guarantees oxygen demand of the arrested normotherm rat-heart only under condition of sufficient coronary flow. The coronary flow during constant-pressure cardioplegic haemoglobin-perfusion was about 58% higher than during cardioplegic perfusion without haemoglobin. Interstitial oedema after perfusion for 1 hour was more intense after perfusion with the conventional cardioplegic solution than after cardioplegic haemoglobin-perfusion. Normothermic ischaemia of 20 minutes after perfusion with cardioplegic haemoglobin-solution was connected with a smaller drop of creatinephosphate and adenosinetriphosphate than after conventional cardioplegic perfusion, electron-optical preparations showed smaller changes of the structure. The use of cardioplegic haemoglobin-solutions in heart-surgery and the advantages of cardioplegic haemoglobinperfusion are discussed.
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002302:Cardiac Output / Q000187:drug effects; D006321:Heart / Q000187:drug effects; D006324:Heart Arrest, Induced; D006454:Hemoglobins; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D010100:Oxygen / Q000378:metabolism; D010477:Perfusion / Q000379:methods*; D010725:Phosphocreatine / Q000032:analysis; D051381:Rats
D006454:Hemoglobins; D010725:Phosphocreatine; D000255:Adenosine Triphosphate; D010100:Oxygen
Myokardprotektion durch kardioplegische Haemoglobinperfusion. Untersuchungen an isolierten Rattenherzen.