Histofluorescence technique for the intracellular localization of chemical carcinogens.
Many carcinogens exhibit a fluorescent emission when excited with ultraviolet light. Advantage has been taken of this property to develop a technique that can detect microquantities of these carcinogens on conventional microscopic tissue preparations. This work describes the localization of aflatoxin B1, N-2-fluorenylacetamide and benzo(a)pyrene both in the cell cytoplasm and nucleus after in vivo administration of these compounds.
D015073:2-Acetylaminofluorene / Q000032:analysis; D000348:Aflatoxins / Q000032:analysis; D000818:Animals; D001580:Benzopyrenes / Q000032:analysis; D002273:Carcinogens / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis*; D003593:Cytoplasm / Q000032:analysis*; D005260:Female; D006651:Histocytochemistry / Q000379:methods*; D008856:Microscopy, Fluorescence; D051381:Rats
D000348:Aflatoxins; D001580:Benzopyrenes; D002273:Carcinogens; D015073:2-Acetylaminofluorene