[Properties of the RNA of the new Okhotsk orbivirus].
Electron microscopic examination of Okhotsky virus RNA showed it to have double-stranded fragmented structure. Investigation of the kinetics of virus-specific RNA synthesis in chick embryo fibroblast cell cultures inoculated at a high multiplicity of infection demonstrated that the synthesis of virus-specific RNAs of Okhotsky virus reached maximum by 7 hours postinfection. A decrease in the multiplicity of infection slowed down the kinetics of virus-specific RNAs synthesis. The sedimentation analysis in sucrose density gradient showed the virus-specific RNAs of Okhotsky virus to sediment with a peak in 14.5 S band.
D000818:Animals; D001103:Arboviruses / Q000254:growth & development / Q000648:ultrastructure; D002460:Cell Line; D002642:Chick Embryo; D005347:Fibroblasts; D066298:In Vitro Techniques; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis
D012367:RNA, Viral
Izuchenie nekotorykh svoĭstv RNK novogo orbivirusa Okhotskiĭ.