Detection of alkaline phosphatase/immunoglobulin complexes.
We report another patient with a circulating alkaline phosphatase/immunoglobulin complex in his blood, and describe a simple method of demonstrating such complexes. On electrophoresis on cellulose acetate, the complex was relatively slow moving and there was no activity in the normal bone/liver isoenzyme region. When the serum was treated with trypsin, the slow band disappeared and the normal pattern was restored.
D000368:Aged; D000469:Alkaline Phosphatase / Q000097:blood*; D000936:Antigen-Antibody Complex / Q000032:analysis*; D001842:Bone and Bones / Q000201:enzymology; D055598:Chemical Phenomena; D002621:Chemistry; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification; D008099:Liver / Q000201:enzymology; D046911:Macromolecular Substances; D008297:Male; D014357:Trypsin
D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D007527:Isoenzymes; D046911:Macromolecular Substances; D000469:Alkaline Phosphatase; D014357:Trypsin