Inhibition of proliferation of cultured cells by polyamine and cysteine interaction.
Both L-cysteine and spermidine oxidation inhibited the proliferation of cultured cells when these reagents were added in low concentrations. Higher concentrations (above 1 mM) of cysteine were not cytotoxic. Furthermore, high concentrations of L-cysteine partially protected against spermidine oxidation toxicity. D-cysteine on its own behaved exactly the same as an equimolar concentration of L-cysteine, but instead of protecting against the effects of spermidine oxidation, the inhibition was enhanced. A possible mechanism for the interaction is discussed.
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D003545:Cysteine / Q000494:pharmacology*; D004347:Drug Interactions; D008545:Melanoma / Q000473:pathology; D051379:Mice; D013095:Spermidine / Q000494:pharmacology*
D003545:Cysteine; D013095:Spermidine