Isocratic high-performance liquid chromatography separation of phosphoglycerides and lysophosphoglycerides.
An isocratic, rapid method for separation of phospho- and lysophospholipids by high-performance liquid chromatography employing acetonitrile, methanol and water as the mobile phase is described. Separation is achieved on a silica based cation-exchange column with individual components monitored directly by UV absorbance at 203 nm. In solutions of phospho- and lysophospholipid standards and in extracts of rabbit myocardium, recovery of individual components exceeds 95%. With the exception of phosphatidyl serine and phosphatidyl ethanolamine that could not be resolved other major phospho- and lysophospholipid constituents found in extracts of cell membranes are separated completely within 40 min. Retention time for selected moieties can be shortened even further with flow programming.
D000097:Acetonitriles; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005994:Glycerophosphates / Q000302:isolation & purification*; D008563:Membrane Lipids / Q000032:analysis; D000432:Methanol; D009206:Myocardium / Q000032:analysis; D010743:Phospholipids / Q000302:isolation & purification*; D011817:Rabbits
D000097:Acetonitriles; D005994:Glycerophosphates; D008563:Membrane Lipids; D010743:Phospholipids; D000432:Methanol