Variations of some enzyme and coenzyme concentrations in normal human erythrocytes fractionated by velocity sedimentation.
The concentrations of glucose-6-phosphate dehydrogenase, adenosine triphosphate and 2,3-diphosphoglycerate were measured in various fractions of normal human erythrocytes separated by velocity sedimentation in isotonic saline and sucrose buffer based on their tendency to aggregate. The levels of 2,3-diphosphoglycerate varied most drastically in fresh erythrocytes, being lowest in fast sedimenting cells and highest in slowly sedimenting cells. This result implies that the 2,3-diphosphoglycerate concentrations is not constant in all normal human erythrocytes and that 2,3-diphosphoglycerate may be involved in the mechanism of a red cell clumping in vitro.
D000255:Adenosine Triphosphate / Q000097:blood*; D002469:Cell Separation; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D006801:Humans
D004163:Diphosphoglyceric Acids; D000255:Adenosine Triphosphate; D005954:Glucosephosphate Dehydrogenase