American ginseng. II. Analysis of ginsenosides and their sapogenins in biological fluids.
A gas-liquid chromatography (tlc) method was developed to assay individual ginsenosides and sapogenins in rabbit plasma and urine samples. A flavonoid, panasenoside, and a sterol, stigmasterol, were used as internal standards for ginsenosides and their sapogenins, respectively. Linear relationships of peak height ratio to weight ratio were obtained for ginsenosides (A1, 20-350 microgram; A2, 20-400 microgram; B2, 20-300 microgram; C, 20-500 microgram), and sapogenins (panaxadiol or panaxatriol, 10-200 microgram) in 0.1 ml of the silylation mixture. The glc assay method developed was sensitive to 0.2 microgram of ginsenosides and 0.1 microgram of sapogenins.
D000818:Animals; D001826:Body Fluids / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods; D036145:Ginsenosides; D005894:Panax / Q000032:analysis*; D010946:Plants, Medicinal; D011817:Rabbits; D012502:Sapogenins / Q000032:analysis*; D012503:Saponins / Q000032:analysis*
D036145:Ginsenosides; D012502:Sapogenins; D012503:Saponins