Production of verruculogen by Penicillium estinogenum in stirred fermenters.
A spectrofluorometric assay for the estimation of the tremorgenic mycotoxin verruculogen in crude mycelial extract has been devised and used to determine concentrations as low as 0.2 microgram ml-1. Verruculogen production by Penicillium estinogenum has been extended from surface culture to submerged culture in 60 1 stirred fermenters, in which the maximum cell-associated mycotoxin yield [5 mg (100 ml culture)-1] was obtained within 7 d. It was found necessary to supplement the medium (Czapek Dox broth plus 0.5% yeast extract) with calcium chloride (2%) to induce profuse sporulation (2 X 10(7) conidia ml-1).
D002122:Calcium Chloride; D003470:Culture Media; D005285:Fermentation; D009183:Mycotoxins / Q000096:biosynthesis*; D010407:Penicillium / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
D003470:Culture Media; D009183:Mycotoxins; D002122:Calcium Chloride