title: [Determination of 25-hydroxycholecalciferol in the blood by means of protein binding competition].
abstract: A competitive binding-protein assay for 25-hydroxycholecalciferol, is described in which serum from vitamin D deficient rats is used as the source of binding protein. Serum samples after extraction are chromatographed using silicic acid. Bovine albumin is used in the buffer as a protein stabilizer and lipid solubilizer, at a concentration shown to enhance the reproducibility of the assay without interfering in the binding properties of 25-hydroxycholecalciferol. The method appears to be sufficiently sensitive for clinical studies even in vitamin D deficiency.
mesh_terms: D000818:Animals; D006887:Hydroxycholecalciferols / Q000032:analysis / Q000097:blood*; D011485:Protein Binding; D051381:Rats; D014808:Vitamin D Deficiency / Q000378:metabolism*
chemical_list: D006887:Hydroxycholecalciferols
vernacular_title: Dosaggio del 25-idrossicolecalciferolo nel siero mediante competizione di legame proteico.