title: Cell surface interaction induces polarization of mouse 8-cell blastomeres at compaction.
abstract: The development of the polarized surface binding of the fluoresceinated ligand concanavalin A (FITC-Con A) was studied in blastomeres of the early mouse embryo. Single 8-cell blastomeres, natural 8-cell couplets derived from the in vitro division of individual 4-cell blastomeres, and reagregated couplets made from dissociated 8-cells were cultured for varying periods of time and on a variety of substrata. The development of surface polarity was found to be highly dependent upon cell contact. Over 50% of the cells in couplets were polarized after 4-5 hr in culture, with the smaller cell in the couplet usually more advanced in its polarization than the larger cell. The orientation of the poles of FITC-Con A binding was opposite the point of contact between cells in the couplets regardless of their previous orientation within the embryo or the plane of cleavage.
mesh_terms: D000818:Animals; D001757:Blastomeres / Q000502:physiology*; D002448:Cell Adhesion; D002450:Cell Communication; D002462:Cell Membrane / Q000502:physiology; D003208:Concanavalin A / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D009924:Organ Culture Techniques; D013499:Surface Properties
chemical_list: D003208:Concanavalin A