Automatic analysis of neutral sugar components in glycoproteins and complex carbohydrates.
A simple and sensitive automated method has been developed to separate monosaccharides by reverse-phase chromatography using the amino acid analyzer column in the Li+ form with 90% alcohol as eluent. The column can be regenerated with LiBr and 90% ethanol to achieve highly reproducible resolution of sugar components. Glycoproteins and polysaccharides are hydrolyzed by a combination of methanesulfonic acid/Dowex 50 X 8 resin in water. Glucose and mannose are distinguished by treatment of the hydrolysate with glucose oxidase prior to analysis.
D001322:Autoanalysis; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods; D006023:Glycoproteins; D009005:Monosaccharides / Q000032:analysis*; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D013296:Streptococcus pneumoniae / Q000032:analysis
D006023:Glycoproteins; D009005:Monosaccharides; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial