Chemical studies of tissue polypeptide antigen (TPA). II. Partial amino acid sequences of cyanogen bromide fragments of TPA subunit B1.
A subunit (designated B1) of the tumor associated peptide "Tissue Polypeptide Antigen" (TPA) has been cleaved by cyanogen bromide. Some of the fragments contained TPA-activity (as measured by a standardized hemagglutination inhibition method). The fragments have been separated, and partial amino acid sequences have been determined for some of the fragments. A method for purification of the most active fragment (designated BrCN:C) has been worked out from cleavage products obtained by cleavage with cyanogen bromide of "Washed Tissue Powder". By this method fragment BrCN:C has been isolated from a pool of placentae and a pool or carcinoma tumors, and the amino acid sequences of more than 50 amino acids from the N-terminus have been determined. Fragment BrCN:C has been demonstrated from three different sources, which indicates the presence of a common part in subunit B1 independent of source.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000951:Antigens, Neoplasm; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification; D010455:Peptides; D019396:Tissue Polypeptide Antigen
D000596:Amino Acids; D000951:Antigens, Neoplasm; D010446:Peptide Fragments; D010455:Peptides; D019396:Tissue Polypeptide Antigen; D003488:Cyanogen Bromide