Regulation of synthesis of beta-fructofuranosidase (invertase) in Streptococcus mitis.
beta-Fructofuranosidase activity was found to be cell-bound in Streptococcus mitis ATCC 903. The following evidence suggests that induction functions as a regulatory mechanism for beta-fructofuranosidase in S. mitis: (1) on transfer of glucose-grown exponential phase bacteria to sucrose medium, the specific activity of beta-fructofuranosidase increased fourfold in the course of one generation; (2) other sugars had no stimulatory effect on the rate of synthesis of beta-fructofuranosidase; (3) the effect of sucrose on the rate of synthesis of beta-fructofuranosidase could be measured within a few minutes. Glucose, fructose and mannose repressed beta-fructofuranosidase. The addition of glucose to bacteria growing on sucrose repressed beta-fructofuranosidase for about one generation. The intracellular concentration of glucose was considerably increased during repression, while the intracellular concentration of glycolytic intermediates did not vary significantly.
D050260:Carbohydrate Metabolism; D002453:Cell Cycle; D003470:Culture Media; D004794:Enzyme Repression; D005947:Glucose / Q000378:metabolism; D013291:Streptococcus / Q000166:cytology / Q000201:enzymology*; D013393:Sucrase / Q000096:biosynthesis*; D013395:Sucrose / Q000378:metabolism
D003470:Culture Media; D013395:Sucrose; D013393:Sucrase; D005947:Glucose