title: Evaluation of serum creatinine measurement by the direct acidification method for errors contributed by non-creatinine chromogens.
abstract: Serum creatinine determination by the direct acidification method was found to be non-specific. Chromogens other than creatinine could react with alkaline picrate and their developed color was also destroyed by acid. Only elevated levels of bilirubin and acetoacetate caused a significant interfering effect. The degree of this interference depended on: (1) the amount of the interfering substance; (2) the reaction time allowed for color development; and (3) the final acidity for decolorization.
mesh_terms: D000090:Acetoacetates; D001663:Bilirubin; D055598:Chemical Phenomena; D002621:Chemistry; D002863:Chromogenic Compounds; D003404:Creatinine / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D010851:Picrates; D013053:Spectrophotometry
chemical_list: D000090:Acetoacetates; D002863:Chromogenic Compounds; D010851:Picrates; D003404:Creatinine; D001663:Bilirubin