Sequences translated by Balbiani ring 75S RNA in vitro are present in giant secretory protein from Chironomus tentans.
The 75S RNA originating in the large Balbiani rings 1 and 2 (BRI and 2) was isolated and used for in vitro translation in the mRNA dependent reticulocyte lystate. Conditions (K+-concentration, temperature, time etc). were optimized for obtaining translation products of maximal size. Polypeptide chains up to about 500,000 D were obtained but no complete translation products. Trypic fingerprints were performed on the in vitro products as well as on the secretory protein components nos. I and II+III labelled with 35S-methionine. There was a large degree of correspondence between the fingerprint of the in vitro product and that of component I but less to that of component II+III. The results suggest that 75S RNA with an origin in the BR1 and BR2 codes for the giant secretory protein component I.
D000595:Amino Acid Sequence; D000818:Animals; D002683:Chironomidae / Q000235:genetics*; D004175:Diptera / Q000235:genetics*; D005796:Genes; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D012471:Salivary Proteins and Peptides / Q000032:analysis*
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012471:Salivary Proteins and Peptides