Measurement of acetylcholine receptor and anti-receptor antibodies by ELISA.
Both acetylcholine receptor (AChR) and antibodies capable of binding to the AChR can be measured using the recently developed enzyme-linked immunosorbent assay (ELISA). The assay is very sensitive and reproducible. Because of the relative simplicity of the ELISA in comparison to radioimmunoassay procedures currently used to measure anti-AChR antibodies, the ELISA may prove valuable as a clinical diagnostic tool for the human disease myasthenia gravis.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002038:Bungarotoxins / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D018680:Cholinergic Antagonists; D004797:Enzyme-Linked Immunosorbent Assay; D005399:Fishes; D009157:Myasthenia Gravis / Q000175:diagnosis; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D011809:Quinones / Q000494:pharmacology; D011950:Receptors, Cholinergic / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D002038:Bungarotoxins; D018680:Cholinergic Antagonists; D000644:Quaternary Ammonium Compounds; D011809:Quinones; D011950:Receptors, Cholinergic; D002217:Carbachol; C004991:benzoquinonium