Demonstration by a rosette-forming technique of surface receptors for low-density lipoproteins on lymphoblastoid cells.
1. A simple technique of 'rosette' formation by low-density-lipoprotein-coated erythrocytes around human lymphoblastoid cells, stimulated to generate specific high affinity low-density lipoprotein receptors, is described. 2. Rosette formation is specific for high-affinity surface binding alone and the properties of the low-density lipoprotein receptors defined by it are identical with those found by using 125I-labelled low-density lipoprotein binding at 4 degrees C. 3. Preliminary observations suggest that specific surface binding of low-density lipoprotein is shown by some, but not all, lymphoid cell lines, is independent of certain other surface receptors and in unrelated to whether the cells originate from B or T cells. 4. Loss of low-density lipoprotein surface receptors on lymphoid cell lines (as with other surface receptors) may occur simply from prolonged culture rather than as a result of genetically mediated familial abnormality.
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D011485:Protein Binding; D011955:Receptors, Drug / Q000378:metabolism*; D012397:Rosette Formation; D014357:Trypsin
D008077:Lipoproteins, LDL; D011955:Receptors, Drug; D014357:Trypsin