title: Amino acid sequence of cyanogen bromide fragment CN2 from Hong Kong influenza haemagglutinin heavy chain.
abstract: The amino acid sequence of cyanogen bromide peptide CN2 from the heavy chain (HA1) of the haemagglutinin of the Hong Kong variant A/Memphis/102/72 has been obtained by direct, automated sequence analysis on the whole fragment and by manual dansyl-Edman degradation of tryptic, peptic and chymotryptic peptides. It was found to contain 92 amino acid residues, including a large, insoluble, tryptic core peptide (residues 62-87). It did not contain any half-cystine residues or carbohydrate. The determination of its structure was complicated by the presence of an Asn-Ile bond at positions 48-49 which was readily cleaved by both trypsin and chymotrypsin.
mesh_terms: D000595:Amino Acid Sequence; D002850:Chromatography, Gel; D003488:Cyanogen Bromide; D004590:Electrophoresis, Paper; D006389:Hemagglutinins, Viral / Q000032:analysis*; D009980:Influenza A virus / Q000276:immunology*; D010446:Peptide Fragments / Q000032:analysis*
chemical_list: D006389:Hemagglutinins, Viral; D010446:Peptide Fragments; D003488:Cyanogen Bromide