Human melanoma tumor-specific antigens (II): Comparison of antigens from tumor cell plasma membranes of five melanoma patients.
Plasma membranes were purified from tumor tissue of five malignant melanoma patients. The purified membranes were solubilized by sonication in KC1 in the presence of Triton X 100. The soluble membranes that were obtained by this procedure contained human melanoma tumor-specific antigens. The antigens were separated from the other soluble plasma membrane proteins by chromatography on affinity columns made from the patients' tumor-specific pre-absorbed IgG coupled to Sepharose 4B. Specific antigens which were eluted from these affinity columns with potassium thiocyanate were determined by crossover immunoelectrophoresis. The five tumors appear to have a similar complement of melanoma tumor-specific plasma membrane-associated antigens.
D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient; D006801:Humans; D007122:Immunoelectrophoresis; D008545:Melanoma / Q000276:immunology*; D008970:Molecular Weight
D000951:Antigens, Neoplasm; D000954:Antigens, Surface