H-2-dependent cell-mediated immunity in vivo: delayed-type hypersensitivity and contact sensitization induced by fluorescein isothiocyanate-conjugated cells.
Fluorescein isothiocyanate (FITC) was found to be a suitable hapten to study cell-mediated immunity in vivo in mice. Delayed-type hypersensitivity (DTH) footpad swelling reactions were induced and elicited by injection of FITC-conjugated syngeneic spleen cells, and contact sensitivity (CS) was induced and elicited by skin painting with reactive FITC. Cross-reactive specificity between DTH and CS was demonstrated. Thus, DTH induction with FITC-spleen cells (FITC-SC) also led to the ability to elicit CS; and CS induction by skin painting also led to the ability to elicit DTH with FITC-SC. However, the 2 reactions differed in that the ability to elicit DTH induced by subcutaneous immunization with FITC-SC was evanescent, whereas CS induced by skin painting was long lived. Specificity of these reactions was hapten dependent and additionally required homology at the H-2 locus of the major histocompatibility complex. The H-2 specificity requirement was not evanescent.
D000818:Animals; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D005452:Fluoresceins / Q000276:immunology*; D006183:H-2 Antigens; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007114:Immunization; D007116:Immunization, Passive; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology*; D013997:Time Factors
D005452:Fluoresceins; D006183:H-2 Antigens