title: [Conversion of D-glucose into D-fructose in a column reactor with immobilized glucose isomerase].
abstract: Conversion of D-glucose into D-fructose in a column reactor of a continuous type was studied using an immobilized enzyme, glucose isomerase from Actinomyces olivocinereus. The effective parameters of Km, Vmax, energy activation at various flow rates were calculated. During 32 days of continuous operation the enzyme activity fell down to 84% of the initial level. A system of recirculation was employed to use immobilized glucose isomerase with a low activity.
mesh_terms: D000190:Actinomyces / Q000201:enzymology*; D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005632:Fructose; D005947:Glucose; D007700:Kinetics; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D005632:Fructose; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase; D005947:Glucose
vernacular_title: Izomerizatsiia D-gliukozy v D-fruktozy v kolonochnom reaktore s immobilizovannoĭ gliukozoizomerazoĭ.