Recovery of large numbers of eosinophils from mice infected with Toxocara canis.
As a method of recovering large numbers of eosinophils, mice were infected with Toxocara canis which in 12 days produced a high peripheral eosinophilia; the mice were then injected intraperitoneally with an extract of T. canis adult or Anisakis larval worms. Two days after injection of extract, peritoneal exudates were withdrawn, washed, and enumerated; the exudates were observed to be 70--80% eosinophils with a mean total recovery of 1 X 10(8) eosinophils per mouse.
D000818:Animals; D001196:Ascariasis / Q000097:blood*; D001202:Ascitic Fluid / Q000166:cytology; D002634:Chemotaxis, Leukocyte; D004802:Eosinophilia / Q000469:parasitology*; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D014120:Toxocariasis / Q000097:blood*