Glycolipids of rat large intestine. Characterization of a novel blood group B-active tetraglycosylceramide absent from small intestine.
A novel blood group B-active tetraglycosylceramide has been isolated from rat large intestine. It is probably present only in the epithelial cells. Although the compound was not obtained pure, the structure was conclusively established by mass spectrometry, proton NMR spectroscopy and degradative studies to be Galp alpha 1 leads to 3Galp(2 comes from 1 alpha Fucp)beta 1 leads to 4Glcp beta 1 leads to 1Cer. The ceramide was composed of mainly trihydroxy long-chain base (18:0) and non-hydroxy fatty acids (16:0-24:0). The glycolipid was absent from small intestine.
D000818:Animals; D002849:Chromatography, Gas; D006028:Glycosphingolipids / Q000032:analysis*; D007420:Intestine, Large / Q000032:analysis*; D007421:Intestine, Small / Q000032:analysis*; D008297:Male; D013058:Mass Spectrometry; D009928:Organ Specificity; D051381:Rats
D006028:Glycosphingolipids