Antigenic and molecular relationship of human prostatic acid phosphatase isoenzymes.
Human prostate contains two major acid phosphatase isoenzymes, 2 and 4. Isoenzyme 2 is a glycoprotein with slow electrophoretic mobility. Treatment with sialidase increases its electrophoretic mobility to approach that of isoenzyme 4 which contains no carbohydrate. The identical protein structure of isoenzyme 2 and 4 was revealed by their antigenic identity. Serum acid phosphatase isoenzyme 5 is a different protein species and has no antigenic relationship to isoenzymes 2 and 4. Our results indicate that the elevation of isoenzyme 5 in late stages of prostatic carcinoma with bone metastases is attributable to increased osteoclastic activity.
D000135:Acid Phosphatase / Q000032:analysis*; D000941:Antigens / Q000032:analysis*; D006801:Humans; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000032:analysis*; D008297:Male; D011467:Prostate / Q000201:enzymology* / Q000276:immunology
D000941:Antigens; D007527:Isoenzymes; D000135:Acid Phosphatase