Lysis of growing fissin-yeast cells induced by aculeacin A, a new antifungal antibiotic.
Cells of Schizosaccharomyces pombe grown in the presence of aculeacin A, a peptide antibiotic, were lysed resulting the death of cells. Under high osmolarity, the cellular lysis induced by aculeacin A was considerably reduced. The use of synchronous-culture systems distinguished cell elongation from cell division revealed that the sites of aculeacin A-induced lysis on the fission yeast were the end(s) and the cell plate region, corresponded to the regions of the cell wall synthesis. Aculeacin A-resistant survivors exhibited morphological alterations which were swollen at one or both ends of the cell and appeared drumstick or dumbbel like; the wall of the bulge region was observed to be stained with a fluorescent brightner, as well as that of the cell plate region. These effects of aculeacin A are discussed as compared with effects of 2-deoxy-D-glucose.
D000935:Antifungal Agents / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects*; D002473:Cell Wall / Q000187:drug effects; D003847:Deoxyglucose / Q000494:pharmacology; D009994:Osmolar Concentration; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure
D000935:Antifungal Agents; D003847:Deoxyglucose