Analysis of enzymatically isolated adults of Onchocerca volvulus after treatment of patients with suramin or metrifonate.
The application of a new technique is described permitting a better recognition of activities of drugs on macrofilariae of Onchocerca volvulus. In two villages of a hyperendemic focus in Liberia onchocercomata were excised from untreated persons and from patients treated with conventional doses of suramin or metrifonate. The adult worms were enzymatically isolated with collagenase and microscopically examined for degenerative alterations including calcifications and for the presence of eggs and embryos in the female parasites. Six weeks after the last dose of suramin the degenerative alterations could not be distinguished qualitatively from those in untreated worms, but a statistically significant macrofilaricidal efficacy could be shown by quantitative analyses, although more than half of the parasites were still alive at this early date. Metrifonate did not show any macrofilaricidal effect.
D000818:Animals; D005260:Female; D006801:Humans; D008297:Male; D009854:Onchocerca / Q000187:drug effects* / Q000302:isolation & purification; D009855:Onchocerciasis / Q000188:drug therapy; D013498:Suramin / Q000494:pharmacology* / Q000627:therapeutic use; D014236:Trichlorfon / Q000494:pharmacology* / Q000627:therapeutic use
D013498:Suramin; D014236:Trichlorfon