[Experimental examinations on forensic determination of time of death by electrofocusing of soluble muscle protein (author's transl)].
Proteolytic changes in the muscles of cadaver are demonstrable by putrefaction experiment with the electrofocusing on polyacrylamide gels. These changes can--within a narrow limit--be used in the determination of time of death at a stage when the early signs of death are not suitable any more. A fraction with a pI-value of 7.2 appears as a proteolytic fission at 30 degrees C not earlier than the second day after death. This fraction can be detected at 20 degrees C from the fifth to the sixth day p.m. At 10 degrees C it will not be detectable until the ninth day p.m. At a pH-range between 6.9 and 7.7, at 30 degrees C and 8 days after death, or at 20 degrees C and 9 days after death, only a relatively stable fraction is detectable with a pI-value between 6.9 and 7.0.
D005554:Forensic Medicine; D006801:Humans; D007525:Isoelectric Focusing; D009124:Muscle Proteins / Q000032:analysis*; D011180:Postmortem Changes; D013696:Temperature
D009124:Muscle Proteins
Experimentelle Untersuchungen zur forensischen Liegezeitbestimmung durch Elektrofokussierung von löslichen Muskelproteinen.