Isolation procedure for bovine retinal rod outer segments.
An isolation procedure to obtain rod outer segments from cattle retinas is reported. Centrifugation of homogenates in discontinuous and continuous sucrose density gradients yields purified photoreceptor cell outer segments. Assay of the final preparation for rhodopsin content gives a ratio of 2.4 for DO280nm/DO498nm.
D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient; D010786:Photoreceptor Cells / Q000032:analysis*; D012243:Rhodopsin / Q000302:isolation & purification; D012374:Rod Cell Outer Segment / Q000032:analysis*
D012243:Rhodopsin