Triple-lumen perfusion of the canine jejunum.
The triple-lumen gut perfusion technique was validated in five dogs with established jejunal fistulas. Transit time through the 30-cm test segment was 6.2 min when perfused at 4 ml/min with a balanced electrolyte solution (Na 140, K 5, Cl 110 and HCO3 35 mM), and this remained constant during a 3-hr test period. Fasting intestinal content was very small in the dog. After 35 min equilibration, steady-state absorption was achieved during perfusion with electrolyte solution containing polyethylene glycol (5 g/liter) as a nonabsorbable marker (10 studies). The triple-lumen gut perfusion technique is well suited to the study of intestinal transport in vivo in dogs with chronic intestinal fistulas.
D000818:Animals; D001692:Biological Transport; D004285:Dogs; D005215:Fasting; D005260:Female; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000378:metabolism*; D007441:Intubation, Gastrointestinal / Q000379:methods; D007583:Jejunum / Q000378:metabolism*; D010477:Perfusion / Q000379:methods*; D011092:Polyethylene Glycols; D011506:Proteins / Q000378:metabolism; D013997:Time Factors; D014882:Water-Electrolyte Balance
D011506:Proteins; D011092:Polyethylene Glycols