title: Action of barbiturates on activity of acetylcholinesterase from synaptosomal membranes.
abstract: The acetylcholinesterase from synaptosomal membranes is inhibited by anesthetics: Nembutal, brietal, and thiopental. Nembutal and brietal decrease the Km for acetylthiocholine, without changes in Vmas. A noncompetitive type of inhibition is produced by thiopental. This anesthetic decreases Arrhenius plot discontinuity by about 4 degrees C and increases activation energies. Nembutal and brietal do not change Arrhenius plot discontinuities, but they increase activation energies. These results suggest that barbiturates change lipid-protein interactions in synaptosomal membranes.
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D007700:Kinetics; D008297:Male; D008723:Methohexital / Q000494:pharmacology*; D010424:Pentobarbital / Q000494:pharmacology*; D051381:Rats; D013570:Synaptic Membranes / Q000201:enzymology*; D013874:Thiopental / Q000494:pharmacology*
chemical_list: D008723:Methohexital; D000110:Acetylcholinesterase; D010424:Pentobarbital; D013874:Thiopental