title: Species specific decondensation of human spermatozoa nuclei by heparin.
abstract: Decondensation of human spermatozoa nuclei has been induced by exposure of intact spermatozoa to heparin, while the spermatozoa of rabbit, ram, and bull remained highly condensed under similar experimental conditions. This process occurred in the complete absence of any disulfide bond cleaving reactant. Swelling of human spermatozoa nuclei commenced about 30 min after the addition of heparin and depended on heparin concentration reaching 83% of swelled nuclei after 6 hr. of incubation with 5000 USP of heparin per ml. Addition of 10 mg/ml of trypsin soybean inhibitor did not interfere with the swelling action of heparin. Electron microscope observations of human spermatozoa nuclei treated with heparin revealed that the chromatin is organized into "hublike" nuclear bodies joined by a network of cross-linked and branched chromatin fibers ranging in thickness from 25 to 1.5 nm.
mesh_terms: D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000187:drug effects*; D002843:Chromatin / Q000648:ultrastructure; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D008297:Male; D011817:Rabbits; D012756:Sheep; D013094:Spermatozoa / Q000187:drug effects*; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000494:pharmacology
chemical_list: D002843:Chromatin; D006493:Heparin; D014360:Trypsin Inhibitor, Kunitz Soybean