Tumor pathophysiology: the perfusion model.
A procedure for perfusing a tumor as an isolated organ separated from the host is described. Details of the following steps have been outlined: preparation of a tumor connected to the host by a single artery and vein, transfer of the tumor from the host to the ex vivo perfusion system, constitution of the perfusion circuit, composition of the perfusates, control of vascular and metabolic parameters during perfusion.
D000818:Animals; D001783:Blood Flow Velocity; D001794:Blood Pressure; D002279:Carcinoma 256, Walker / Q000098:blood supply / Q000503:physiopathology; D004195:Disease Models, Animal; D005260:Female; D005947:Glucose / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000098:blood supply / Q000503:physiopathology; D009369:Neoplasms / Q000098:blood supply / Q000503:physiopathology*; D010101:Oxygen Consumption; D010477:Perfusion / Q000295:instrumentation / Q000379:methods*; D051381:Rats
D005947:Glucose