Primary in vitro antibody response in human cord blood lymphocytes.
Human cord blood lymphocytes were stimulated with trinitrophenyl--polyacrylamide beads (TNP--PAA) in order to induce a primary IgM anti-TNP response. With few exceptions, no anti-TPN response was obtained, whereas peripheral blood lymphomonocytic cells (PBL) from 18-mth-old children were able to respond to TNP-PAA. The addition of lipopolysaccharide (LPS) or of mitomycin-treated adult PBL to cultures of cord blood lymphocytes significantly enhanced their anti-TNP response, thus showing that functional anti-TNP B cell precursors are present in the human neonate.
D000180:Acrylic Resins / Q000276:immunology; D000917:Antibody Formation; D002478:Cells, Cultured; D002675:Child, Preschool; D005312:Fetal Blood / Q000276:immunology*; D006241:Haptens / Q000276:immunology; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology*; D008937:Mitomycins / Q000494:pharmacology; D014301:Trinitrobenzenes / Q000276:immunology; D000099166:Polyacrylamides
D000180:Acrylic Resins; D006241:Haptens; D008070:Lipopolysaccharides; D008937:Mitomycins; D014301:Trinitrobenzenes; D000099166:Polyacrylamides