Lysis of antibody coated chicken erythrocytes by a non-lymphocyte pig blood leukocyte.
Antibody-dependent cellular-cytotoxicity (ADCC) was studied by the 51chromium release test after a 18 h incubation of non-immune porcine blood leukocytes with labelled chicken erythrocytes sensitized with pig antibodies. It was shown that ADCC was inhibited by incubation of leukocytes with aggregated immunoglobulins. In our assay, pig lymphocytes were shown to be inactive whereas non-lymphocyte pig leukocytes are effector cells even at low effector:target ratios.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002645:Chickens / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D010587:Phagocytosis; D013552:Swine / Q000276:immunology*; D013696:Temperature