title: A difference in surface proteins of Fasciola hepatica larvae from intact and nude mice.
abstract: Larvae of the trematode, Fasciola hepatica, dissected from the livers of intact mice at 12 to 14 days of infection, bind anti-immunoglobulins in vitro. This is not so for larvae harvested from the livers of infected hypothymic nude (nu/nu) mice. When attempts were made to confirm the presence of surface Ig using lactoperoxidase-catalysed radioiodination and analysis by SDS-polyacrylamide gel electrophoresis, proteolysis at the time of solubilization markedly affected the profiles obtained. Provided protease inhibitors were present during processing, a mu-like Ig heavy chain and a gamma-like Ig heavy chain could be demonstrated on larvae from nu/+ mice but not on larvae from nu/nu mice.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000469:parasitology; D005260:Female; D007136:Immunoglobulins / Q000032:analysis*; D007814:Larva / Q000276:immunology; D008099:Liver / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011480:Protease Inhibitors / Q000494:pharmacology
chemical_list: D000954:Antigens, Surface; D007136:Immunoglobulins; D011480:Protease Inhibitors