Estimation of the in vivo translational activity of rat liver mitochondria without use of an antibiotic.
In order to estimate the in vivo translational activity of mitochondria without using cycloheximide, we developed a selective separation procedure for the mitochondrial translation products. The effects of cycloheximide and isotopic dilution kinetics on the precursor leucine pool indicate that the pH 11.5 insoluble mitochondrial fraction is greatly enriched in mitochondrial translation products.
D000818:Animals; D002364:Caseins / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D004032:Diet; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008433:Mathematics; D008930:Mitochondria, Liver / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects*; D051381:Rats
D002364:Caseins; D003513:Cycloheximide; D007930:Leucine