title: Membrane cholesterol depletion and K+ transport in high and low potassium sheep red cells.
abstract: The effect of cholesterol depletion on potassium tracer fluxes was studied in sheep red cells. Removal by the plasma incubation method (5, 12, 30) of approximately 31 and 34% membrane cholesterol from high-potassium (HK) and low-potassium (LK) sheep red cells, respectively, did not induce significant changes in the steady-state cation composition of these cells nor in their passive (leak) and active (pump) K+ influxes. In cholesterol-depleted LK sheep red cells, there was no impairment nor augmentation of the Lp an antibody stimulated K+ pump flux and L1-antibody-mediated reduction of K+ leak flux indicating that the removed cholesterol does not contribute to the activity of the Lp and L1 antigens.
mesh_terms: D000818:Animals; D000906:Antibodies; D000941:Antigens / Q000032:analysis; D001692:Biological Transport; D002784:Cholesterol / Q000097:blood*; D004910:Erythrocyte Membrane / Q000276:immunology / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D066298:In Vitro Techniques; D008563:Membrane Lipids / Q000097:blood*; D009996:Osmotic Fragility; D011188:Potassium / Q000097:blood*; D012756:Sheep
chemical_list: D000906:Antibodies; D000941:Antigens; D008563:Membrane Lipids; D002784:Cholesterol; D011188:Potassium