title: Histochemical, autoradiographic and electron microscopic investigations of the renal proximal tubule of male and female rats after castration.
abstract: After castration of 90-day-old male and female rats, changes appear in the renal proximal tubule. A distinction can be made between early changes (up to 10th postoperative day) and later changes (20th-30th postoperative day). Between the 3rd and 5th day after castration the kidney of the females shows an increase in free estrogen receptors (biochemical studies) which are localized in the pars contorta of the proximal tubule (autoradiographic studies), while the male kidney shows a marked increase in urinary protein excretion up to the 10th day after castration. Proximal tubule changes detectable histochemically and electron microscopically do not appear until day 20 or 30 after castration. The results of castration are similar in segments S1 and S2. By days 20 and 30 after castration there is a decrease in the activity of lysosomal enzymes (acid phosphatase, acid beta-galactosidase). Electron microscopy shows a conspicuous decrease in the number of giant lysosomes (mainly in females) and apical vacuoles (mainly in males). A marked increase in the number of lysosomes is found in the S3 segment; females always have more lysosomes than males. The number of peroxisomes is also greatly increased; they appear circular in the females but can assume bizarre shapes in the males. Lipid droplets appear in the basal region of the tubule cell of segments S2 and S3 in the males. The sex differences are preserved in all segments even after castration and become even more pronounced in the S3 segment.
mesh_terms: D000818:Animals; D002369:Castration; D005260:Female; D006867:Hydrolases / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism* / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008871:Microvilli / Q000201:enzymology; D009940:Organoids / Q000648:ultrastructure; D011507:Proteinuria; D051381:Rats; D011960:Receptors, Estrogen / Q000378:metabolism*
chemical_list: D011960:Receptors, Estrogen; D006867:Hydrolases