Isolation of a morphologically-transformed epithelial cell-line from rat kidney following an in vivo dose of dimethylnitrosamine.
In an in vivo-in vitro system, a rat kidney epithelial cell-line was isolated into continuous culture 48 h after a single administration intraperitoneally of dimethylnitrosamine (DMN). The dose used, 60 mg/kg, was known to induce approximately a 40% incidence of renal cortical epithelial umors, in addition to a much higher incidence of renal mesenchymal tumors. The cell-line was established from several persisting islands of epithelial cells which were cultured in Leibowitz medium supplemented with various-growth enhancing agents and periodically, the antifibroblastic agent cishydroxyprolin. Through succeeding subcultures, the cells retained an epithelial morphology and at confluence piled-up into 3-dimensional ridges and domes above the monolayer. The cells were characterized by continuous growth in culture, the ability to form colonies at very low seeding rates, loss of anchorage-dependence as determined by growth in semi-solid media and formation of multicellular aggregates in suspension culture, and agglutination in the presence of conconavalin A. These altered properties of growth and behavior indicate that the renal epithelial cells constitute a morphologically transformed line. As such, they may represent the same target cell population from which DMN-induced epithelial tumors arise in the rat kidney.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D004128:Dimethylnitrosamine; D004848:Epithelium; D005260:Female; D007668:Kidney; D051381:Rats
D004128:Dimethylnitrosamine