title: Evidence for intracistronic complementation of the product of the influenza virus gene Ptra (P3 of fowl plague virus).
abstract: The ts lesion of the fowl plague virus (FPV) mutants ts 18 and ts 236 has been located in RNA segment 2 (Ptra gene, corresponding to P3). After double-infection with these mutants and ts 90 or ts 93, which also carry a ts lesion in segment 2, plaques were formed at the non-permissive temperature (40 degrees C). These plaques cannot be passaged at 40 degrees C and exhibit a morphology which differs from those formed by the wild-type virus. The yield of infectious particles after double-infection shows a non-linear correlation between the plaque number and dilution, indicating that at least two particles are needed for infection of a cell. All experimental evidence points to an intracistronic complementation within the P3 protein.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D005796:Genes; D005814:Genes, Viral; D005816:Genetic Complementation Test; D009980:Influenza A virus / Q000235:genetics* / Q000254:growth & development; D009154:Mutation; D011995:Recombination, Genetic; D013696:Temperature; D010948:Viral Plaque Assay