Phospholipase C-induced neural tube defects in the mouse embryo.
Mouse embryo neurulae were exposed in vitro to phospholipase C to examine the role of carbohydrate-rich extracellular material (ECM) during neurulation. Exposure of embryos to this agent for 12 h resulted in failure of closure of the neural tube. Ultrastructural examination revealed an absence of ECM from regions of the neural tube which failed to close.
D000818:Animals; D004622:Embryo, Mammalian; D005260:Female; D051379:Mice; D009432:Neural Crest / Q000187:drug effects / Q000648:ultrastructure; D009436:Neural Tube Defects / Q000139:chemically induced*; D010740:Phospholipases / Q000494:pharmacology; D011247:Pregnancy; D010738:Type C Phospholipases / Q000494:pharmacology
D010740:Phospholipases; D010738:Type C Phospholipases