title: A new approach to detection of antigen-antibody complexes by microcalorimetric measurements of heat production in blood cells.
abstract: Using batch microcalorimeters of the thermopile conduction type, heat production was measured in human blood in the presence of defined antigens and their specific antibodies. A large increase in heat production, reflecting increased metabolic activity in the blood cells, was found when antigens and specific antisera at certain antigen-antibody proportions were mixed with the blood. The reproducibility and specificity of the method were found to be very good. The present results indicate that calorimetry may be a useful technique for detection of antigen-antibody complexes.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D001773:Blood Cells / Q000276:immunology* / Q000378:metabolism; D002151:Calorimetry; D006358:Hot Temperature; D006801:Humans; D010047:Ovalbumin / Q000276:immunology; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology; D013997:Time Factors
chemical_list: D000936:Antigen-Antibody Complex; D012709:Serum Albumin; D010047:Ovalbumin