In search of optimum conditions for the measurement of creatine kinase activity: a critical review of nineteen formulations.
We compare the reagent composition recommended by six different groups including three European societies for the determination of creatine kinase activity in serum using the coupled hexokinase/glucose-6-phosphate dehydrogenase (EC 2.7.1.1/1.1.1.49) reactions. Even though discrepancies exist between these methods, there are, nevertheless, major areas of consensus which permit a reasonable extrapolation of an approximate composition for optimum response. We then ascertain how reagents used in thirteen commercial kits differ from these approximated optimum conditions. Except for four companies, all the reagent compositions differ remarkably from the conditions recommended by the six groups.
D000244:Adenosine Diphosphate; D000263:Adenylate Kinase / Q000037:antagonists & inhibitors; D003402:Creatine Kinase / Q000032:analysis*; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D006801:Humans; D007202:Indicators and Reagents; D008274:Magnesium / Q000008:administration & dosage; D010725:Phosphocreatine; D011933:Reagent Kits, Diagnostic; D013439:Sulfhydryl Reagents / Q000008:administration & dosage
D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic; D013439:Sulfhydryl Reagents; D010725:Phosphocreatine; D000244:Adenosine Diphosphate; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D003402:Creatine Kinase; D000263:Adenylate Kinase; D008274:Magnesium