A 1H-NMR investigation of the mechanism for the ionophore activity of the bile salts in phospholipid vesicular membranes and the effect of cholesterol.
A 1H-NMR method previously applied to ionophore-medicated transport is used to investigate a similar behaviour by the bile salts. The permeability of phosphatidylcholine vesicles to Pr3+ is increased by several orders of magnitude over the self-diffusion rate and the kinetics indicate a transbilayer movement of inverted micelles [Pr(bile salt)4]. In vesicles containing 40 mol% cholesterol the mechanism of permeability is radically altered and the di- and trihydroxy bile salts behave differently.
D001647:Bile Acids and Salts / Q000494:pharmacology*; D002784:Cholesterol / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology*; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D010539:Permeability; D010713:Phosphatidylcholines; D011221:Praseodymium / Q000378:metabolism
D001647:Bile Acids and Salts; D007476:Ionophores; D008081:Liposomes; D008823:Micelles; D010713:Phosphatidylcholines; D002784:Cholesterol; D011221:Praseodymium