Biological activities of C5a and C5adesArg from hog serum.
The complement-derived peptides C5a and C5adesArg (highly purified from yeast-activated hog serum) were both active in the following biological assays: in aggregation of human leukocytes (potency ratio for C5a:C5adesArg 3:1), in aggregation of guinea pig platelets (10:1), in chemotaxis of peritoneal rabbit leukocytes (10:1), and in contraction of isolated guinea pig ileum (1.4:1). The fact that C5adesArg preparations have 30 and 40% of the activity of C5a in leukocyte aggregation and smooth muscle contraction but only 10% of C5a chemotactic activity, clearly indicates that aggregation and spasmogenic activity are inherent properties of C5adesArg. Evidence that C5adesArg owns all activities studied is further given by the following findings: chromatographic separation of mixtures of C5a and C5adesArg results in the appearance of two peaks of spasmogenic activity; treatment of C5adesArg with carbopeptidase B does neither lead to release of detectable arginine nor abrogate any of the biological effects; treatment of C5a with carboxypeptidase B reduces the activities quantitatively to those of C5adesArg, and liberates the theoretically expected amount of arginine.
D000596:Amino Acids; D000818:Animals; D002268:Carboxypeptidases / Q000494:pharmacology; D002449:Cell Aggregation; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002633:Chemotaxis; D003182:Complement C5; D006168:Guinea Pigs; D006801:Humans; D007082:Ileum; D007962:Leukocytes; D010974:Platelet Aggregation; D011817:Rabbits; D013035:Spasm / Q000209:etiology; D013552:Swine
D000596:Amino Acids; D003182:Complement C5; D002268:Carboxypeptidases