An improved automated fluorimetric method for determination of histamine.
The automated continuous flow system for the extraction and fluorimetric analysis of histamine based on the principle of Shore et al. (1959) has been improved. With lower consumption of reagents and further simplification of the working conditions, histamine can be determined quantitatively in a routine fashion in aqueous samples, with or without protein content, up to a concentration of approximately 0.1 ng/ml. The rate of analysis is 30 samples/h.
D000818:Animals; D005470:Fluorometry / Q000379:methods*; D006168:Guinea Pigs; D006632:Histamine / Q000032:analysis*; D006801:Humans; D007962:Leukocytes / Q000032:analysis; D008168:Lung / Q000032:analysis; D008407:Mast Cells / Q000032:analysis; D051381:Rats
D006632:Histamine