Protein aggregation in aqueous casein solution; effect of irradiation, dose level, concentration, storage, and additives (carbohydrate and lipid).
Radiation-induced aggregation of protein in aqueous solutions was studied. Different concentrations of casein solutions have been irradiated as such or in the presence of carbohydrate and/or lipid. Gel chromatography data indicated a close relation between the amounts of aggregates and protein concentration. Protein aggregation increased with the increase in radiation dose level. The addition of carbohydrate (trehalose) reduced the amount of radiation-induced aggregates, whereas the sole addition of oil caused an induction in protein aggregation when the solution was irradiated at 1 Mrad. However, insignificant changes in protein aggregation were observed when emulsified oil was added to the casein-trehalose solution.
D002364:Caseins / Q000528:radiation effects*; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D003037:Cobalt Radioisotopes; D003720:Densitometry; D004307:Dose-Response Relationship, Radiation; D006368:Helianthus; D009821:Oils; D013997:Time Factors; D014199:Trehalose
D002364:Caseins; D003037:Cobalt Radioisotopes; D009821:Oils; D014199:Trehalose