Uniqueness of sperm mtDNA as compared to somatic mtDNA in ram.
Sperm mtDNA exhibits unique physicochemical properties compared with mtDNA from somatic tissues in the ram. The buoyant density of sperm mtDNA was 1,6983 g/cm3 while the brain, heart and liver mtDNAs was about 1.7005 g/cm3. The Tm of the liver and sperm mtDNA was 71.0 degrees C and 69.5 degrees C, respectively. The G + C content of sperm mtDNA was 3.0 moles % lower than the liver mtDNA. The contour length of the circular sperm mtDNA was 5.01 micrometers compared with the liver mtDNA with contour length of 5.33 micrometers.
D000818:Animals; D001923:Brain Chemistry; D002499:Centrifugation, Density Gradient; D004272:DNA, Mitochondrial / Q000032:analysis*; D006358:Hot Temperature; D008297:Male; D008929:Mitochondria, Heart / Q000032:analysis; D008930:Mitochondria, Liver / Q000032:analysis; D009691:Nucleic Acid Denaturation; D012756:Sheep; D013094:Spermatozoa / Q000032:analysis*
D004272:DNA, Mitochondrial