title: Effect of the conceptus on quantitative and qualitative aspects of uterine secretion in pigs.
abstract: Endometrial explants were removed from uteri of animals pregnant and pseudopregnant (5 mg oestradiol valerate on Days 11--15) for 60 days, from the gravid and non-gravid horns of unilaterally pregnant pigs at Day 60 of pregnancy and fron non-pregnant animals at Day 12 of the oestrous cycle. The tissues were cultured in the presence of L-[34S]methionine for 24 h, and the tissues and medium were then analysed separately by two-dimensional polyacrylamide gel electrophoresis. Radioactive polypeptides were identified by autoradiography of dried gels. Tissues from all all except the cyclic animals released an identical group of polypeptides into the culture medium: the major radioactive products were 4 acidic polypeptides of low molecular weight and several basic proteins, which included the purple phosphatase uteroferrin, and lysozyme. In separate experiments explants from 3 unilaterally pregnant pigs were cultured with L-[3H]leucine, and, on a fresh weight basis, the tissue from the non-gravid horn released significantly less radioactive macromolecular material into the medium in 24 h than did tissue from gravid horns. It therefore appears that although the nature of the secretion produced by the pregnant uterus is a consequence of maternal hormonal regulation alone, the tissue underlying a conceptus is quantitatively more active than that from unoccupied regions of a uterus.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005333:Fetus / Q000502:physiology*; D006023:Glycoproteins / Q000378:metabolism; D011247:Pregnancy; D011270:Pregnancy, Animal; D011555:Pseudopregnancy; D013552:Swine / Q000502:physiology*; D014599:Uterus / Q000201:enzymology / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D000135:Acid Phosphatase