Stimulation of DNA and collagen synthesis by autologous growth factor in cultured fetal rat calvaria.
Conditioned medium derived from organ or cell cultures prepared from 19- to 21-day fetal rat calvaria stimulated the incorporation of [3H]proline collagen and of [3H]thymidine into DNA in organ cultures of the same tissue. Addition of cortisol enhanced the effect on collagen but not on DNA synthesis. These effects appeared to be due to a nondialyzable and heat-stable growth factor.
D000818:Animals; D001846:Bone Development; D001842:Bone and Bones / Q000502:physiology*; D003094:Collagen / Q000096:biosynthesis*; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis; D006133:Growth Substances / Q000302:isolation & purification / Q000502:physiology*; D006854:Hydrocortisone / Q000494:pharmacology; D051381:Rats; D012886:Skull
D006133:Growth Substances; D003094:Collagen; D004247:DNA; D006854:Hydrocortisone