A modification of the gradual fractional trypsinization method for the preparation of rat liver cell cultures.
A new simple technique for the preparation of primary and secondary cultures of rat liver cells is described. Hepatocytes from the liver of 9-day-old rats showed the organotypic morphology, whereas cells from embryonic livers were pleiomorphic.
D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D004622:Embryo, Mammalian; D004847:Epithelial Cells; D008099:Liver / Q000166:cytology*; D051381:Rats; D014357:Trypsin / Q000494:pharmacology
D003470:Culture Media; D014357:Trypsin