Subcellular origin of cholinergic transmitter release from mouse brain.
Samples of minced mouse forebrain were treated in a way that resulted in a high ratio of false cholinergic transmitter (acetylhomocholine) to true transmitter (acetylcholine) in a synaptic vesicle fraction, and a low ratio of false to true transmitter in the nerve terminal cytoplasm. The spontaneous release of cholinergic transmitters from this minced tissue occurred independently of calcium and had a ratio of false to true transmitter similar to that of the cytoplasm, whereas the evoked transmitter release required calcium and had a ratio of false to true transmitter similar to that of the vesicular fraction.
D000109:Acetylcholine / Q000031:analogs & derivatives / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000502:physiology; D003593:Cytoplasm / Q000378:metabolism; D005089:Exocytosis / Q000187:drug effects; D008094:Lithium / Q000494:pharmacology; D008274:Magnesium / Q000502:physiology; D008297:Male; D051379:Mice; D011188:Potassium / Q000494:pharmacology; D013572:Synaptic Vesicles / Q000378:metabolism
D008094:Lithium; D008274:Magnesium; D000109:Acetylcholine; D011188:Potassium; D002118:Calcium