title: Use of unscheduled DNA synthesis in freshly isolated human intestinal mucosal cells for carcinogen detection.
abstract: Mucosal cells freshly isolated from human intestine with pronase retain the capacity to undergo DNA repair synthesis (unscheduled DNA synthesis) during a 2-hr exposure to the carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine, and the procarcinogen, aflatoxin B1. This procedure combining the use of human intestinal mucosal cells and the measurement of unscheduled DNA synthesis may provide a highly relevant and convenient test system for the detection of cell-specific, direct-acting, and activation-dependent chemical carcinogens. The use of whole-cell preparations in such in vitro studies may be of additional significance in view of growing evidence for artefactual metabolism by subcellular fractions.
mesh_terms: D000348:Aflatoxins / Q000633:toxicity; D001345:Autoradiography; D002273:Carcinogens; D002478:Cells, Cultured; D003106:Colon; D004260:DNA Repair; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004848:Epithelium / Q000187:drug effects; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000378:metabolism; D008769:Methylnitronitrosoguanidine / Q000633:toxicity
chemical_list: D000348:Aflatoxins; D002273:Carcinogens; D008769:Methylnitronitrosoguanidine