[Experiments to obtain and use an allergen for the diagnosis of leukemia in animals].
The tested allergen was obtained from a stable cell line FLK which produces leukosis virus type "C" for diagnostics of animal leukosis (cattle, sheep, pigs). It was found that the allergen is strictly specific and induces hypersensitivity of delayed type which is recorded best on the 24th h of injection. The most suitable site to inject the allergen proved to be the tail fold for cattle and sheep and the dorsal part of the ear--for pigs in dose of 0.2 cc. The allergen reaction correlates to a considerable degree with the serologic one (AGDS) but it does not detect all serologic reagents. It detects much larger percent of susceptible animal compared with the hematologic method. Data obtained give grounds to assume that the allergen reaction may be used for quick herd diagnostics of leukosis.
D000485:Allergens / Q000302:isolation & purification*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis; D002478:Cells, Cultured; D007938:Leukemia / Q000175:diagnosis / Q000662:veterinary*; D012190:Retroviridae / Q000276:immunology; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis
D000485:Allergens
Opiti za poluchavane i prilozhenie na alergen za diagnostika na levkozata po zhivotnite.