title: [Comparative studies of the specificity of idiotypic guinea pig and carp antisera].
abstract: The specificity of guinea-pig- and carp-anti-idiotypic antisera were compared by using the radioimmunoassay and the passive hemagglutination inhibition test. Guinea-pigs and carps were immunized with the IgA/k mouse myeloma protein S117 which has binding activity to the hapten N-acetylglucosamine. the resulting antisera were idiotypically specified by absorption and the activity was determined by the binding of the radiolabeled idiotype S117. The binding curves of guinea pig and carp anti-idiotypic antisera are very similar. The idiotype-anti-idiotype reaction could be partially inhibited by the specific hapten N-acetylglucosamine but not by N-acetylgalactosamine. Both antisera, guinea pig and carp, respectively, are different in respect to the immunoglobulin class of anti-idiotypic antibodies which react with the idiotype S117. In the case of the guinea pig antisera the anti-idiotypic antibodies are mainly of the IgG-type whereas the anti-idiotypic antibodies of carp are exclusively of IgM-type. This important difference is discussed.
mesh_terms: D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis*; D000918:Antibody Specificity; D002347:Carps / Q000276:immunology*; D003530:Cyprinidae / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis; D007130:Immunoglobulin Idiotypes / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis; D009194:Myeloma Proteins / Q000276:immunology*; D011817:Rabbits / Q000276:immunology*; D011863:Radioimmunoassay; D013045:Species Specificity
chemical_list: D000912:Antibodies, Neoplasm; D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; D009194:Myeloma Proteins
vernacular_title: Vergleichende Untersuchungen zur Spezifität von idiotypischen Meerschweinchen- und Karpfen-Antiseren.