title: Determination of haloperidol in human serum by radioimmunoassay.
abstract: A radioimmunoassay has been developed which enables accurate determination of haloperidol in human serum. Antiserum was prepared by immunizing guinea pigs with haloperidol (O-carboxymethyl)oxime derivative (III) coupled with bovine serum albumin. With the antiserum, 3H-haloperidol and dextran-coated charcoal, the assay of haloperidol in serum was possible over a concentration range of 1 to 50 ng/ml, using 0.1 ml of human serum without the need of an extraction procedure. Data obtained by radioimmunoassay are in good agreement with those obtained by gas chromatography. No appreciable cross-reactivity was observed neither with haloperidol metabolites nor with other butyrophenone neuroleptics. Serum levels of haloperidol in schizophrenic patients receiving single oral dosing (6 mg/subject) have also been determined.
mesh_terms: D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D006220:Haloperidol / Q000097:blood*; D006241:Haptens / Q000138:chemical synthesis; D006801:Humans; D007114:Immunization; D011863:Radioimmunoassay / Q000379:methods; D012710:Serum Albumin, Bovine
chemical_list: D000906:Antibodies; D006241:Haptens; D012710:Serum Albumin, Bovine; D006220:Haloperidol