Purification of low molecular weight metal-binding proteins by preparative polyacrylamide gel electrophoresis: properties of electrophoretically purified rat liver (Cd, Zn) - metallothioneins.
A method is proposed for purification of metallothionein by preparative polyacrylamide gel electrophoresis. The method enables purification of 100-700 mg of a preparation containing (Cd, Zn) - metallothionein yielding preparations of considerably higher purity as compared with those obtained by ion-exchange chromatography.
D000818:Animals; D002104:Cadmium / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D008099:Liver / Q000032:analysis*; D008667:Metalloproteins / Q000302:isolation & purification*; D008668:Metallothionein / Q000302:isolation & purification*; D011485:Protein Binding; D051381:Rats; D015032:Zinc / Q000378:metabolism
D008667:Metalloproteins; D002104:Cadmium; D008668:Metallothionein; D015032:Zinc