Protein synthesis by bovine chorion and uterine endometrium during the period of implantation.
A grid method for culturing bovine endometrial and chorion tissue is described. A peak of mol. wt 85 000 was obtained only from chorion which had been cultured with intercotyledonary tissue but the co-culture of cotyledon, intercotyledon, kidney or liver with chorion obtained from cows 24-29 days pregnant did not significantly alter the overall level of protein synthesis. The addition of progesterone or oestradiol-17 beta to the culture medium (10(-4) mol/l) significantly depressed protein synthesis by both chorion and uterine endometrium.
D000818:Animals; D002417:Cattle; D002823:Chorion / Q000187:drug effects / Q000378:metabolism*; D046508:Culture Techniques / Q000379:methods; D003864:Depression, Chemical; D010064:Embryo Implantation; D004717:Endometrium / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D007930:Leucine / Q000378:metabolism; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D014176:Protein Biosynthesis; D014599:Uterus / Q000187:drug effects
D011374:Progesterone; D004958:Estradiol; D007930:Leucine