The immobilization technique or solid-phase approach as an aid in investigating the interaction of subunits in oligomeric enzymes, notably of dehydrogenases.
The use of immobilization techniques or a solid-phase approach, allows several questions concerning interactions between sub-units of oligomeric enzymes, particularly dehydrogenases to be, answered are the monomers of ADH and LDH active per se, or are interactions necessary for their activity? Are the sub-units (such as those of ADH) identical from a kinetic point of view? Is the increase in activity due to the addition of ligands (as for GDH), the result of the formation of large aggregates, which appear simultaneously, or is this association a secondary effect? A technique using affinity chromatography, with the help of bifunctional nucleotide derivatives, is presented. This technique could prove useful for topographical studies, or provide new information on the spatial arrangements of the sub-units.
D004800:Enzymes, Immobilized; D008722:Methods; D008954:Models, Biological; D010088:Oxidoreductases / Q000378:metabolism*
D004800:Enzymes, Immobilized; D010088:Oxidoreductases