Immunological properties of malignant and nonmalignant sublines of L-cells.
Selection for immunoresistance and malignancy of nonmalignant sublines of L-cells resulted in the procurement of malignant LS-cells which grew successfully and killed 70% of syngeneic C3H mice. Preliminary inoculation with L-cells protected 30% of the animals from subsequent challenge by LS-cells. The L- and LS-cells had the following immunological properties in common: (a) sensitivity to the complement lytic action; (b) the ability to absorb lymphocytes on their surfaces; (c) the ability to affect the immune reactions by metabolic products; and (d) sensitivity to cytotoxicity of macrophages. However, LS-cells appeared to be more resistant to lysis by alloimmune T-lymphocytes and were much more resistant to lysis by natural killer cells from Wistar rats and athymic nude mice. In syngeneic recipients, the immune response to L- and LS-cells, evaluated by the in vitro cytotoxicity test, was equal for both types of cells. Only a partial cross-reactivity between L- and LS-cells was noted in cellular and humoral immune reactions.
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000276:immunology*; D003165:Complement System Proteins / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D007111:Immunity, Cellular; D007114:Immunization; D007694:Killer Cells, Natural / Q000276:immunology; D007739:L Cells / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology* / Q000473:pathology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology / Q000473:pathology; D051381:Rats; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic
D007106:Immune Sera; D003165:Complement System Proteins