A phase diagram of the binding of mismatched duplex DNAs to hydroxyapatite.
The hydroxyapatite binding properties of imperfectly base-paired DNA have been investigated and summarized in a phase diagram. This diagram defines the conditions at which thermal elution results in both denaturation and desorption of mismatched duplex DNA from hydroxyapatite.
D001482:Base Composition; D002845:Chromatography; D004247:DNA; D004277:DNA, Single-Stranded; D005260:Female; D006358:Hot Temperature; D006801:Humans; D006882:Hydroxyapatites; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes; D009695:Nucleic Acid Renaturation; D011247:Pregnancy
D004277:DNA, Single-Stranded; D006882:Hydroxyapatites; D009692:Nucleic Acid Heteroduplexes; D004247:DNA