A model for the study of granulocyte concentrate preservation.
An inexpensive model system for the study of granulocyte concentrate preservation has been evaluated. Concentrates were collected from the same donors within one hour by manual and automated pheresis techniques. In both types of concentrates, phagocytosis, respiratory burst, microbial killing and cell migration were normal within four hours of collection. Granulocyte function defects induced by 24- to 48-hour storage were similar in both types of concentrates; consequently, granulocyte concentrates collected as described here should serve as a useful model for the investigation of granulocyte concentrate preservation.
D001770:Blood Bactericidal Activity; D001793:Blood Preservation; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte; D006098:Granulocytes / Q000166:cytology; D006801:Humans; D008163:Luminescent Measurements; D008954:Models, Biological; D010101:Oxygen Consumption; D010587:Phagocytosis