Nuclear deprimerones in rat liver and Novikoff hepatoma.
Extraction of total low molecular weight peptides controlling transcription (deprimerones) from rat liver and Novikoff hepatoma nuclei was perfromed with 80% ethanol at pH 9.5. The extracted material was fractionated on a Sephadex G-25 column and active peptidic fractions were collected as Sephadex fraction II of mol. wt. between 1600 and 600. The yield of this peptide fraction was 600 micrograms/mg DNA from rat liver nuclei and 200 micrograms/mg DNA from Novikoff hepatoma nuclei. The results confirm a previously found decrease in chromatin deprimerones in tumor cells using different experimental approach and remain in accordance with the postulated deprimerone theory of carcinogenesis.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002850:Chromatography, Gel; D004247:DNA / Q000378:metabolism; D008099:Liver / Q000166:cytology*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism*; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D010455:Peptides; C021657:deprimerones; D004247:DNA