Salt induced transitions of chromatin core particles studied by tyrosine fluorescence anisotropy.
Chromatin core particles containing 146 base pairs of DNA have been found to undergo a single defined transition below 10 mM ionic strength as studied by both sedimentation velocity and tyrosine fluorescence anisotropy. A method is described for the preparation of such core particles from chicken erythrocytes with greater than 50% yield.
D000818:Animals; D001482:Base Composition; D002645:Chickens; D002843:Chromatin / Q000648:ultrastructure*; D004912:Erythrocytes / Q000032:analysis; D005260:Female; D005454:Fluorescence Polarization; D006657:Histones / Q000097:blood; D007700:Kinetics; D009707:Nucleosomes / Q000032:analysis; D009994:Osmolar Concentration; D012492:Salts; D014443:Tyrosine / Q000032:analysis*
D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; D012492:Salts; D014443:Tyrosine