A novel method for purification of poly(ADP-ribose).
A novel method for the purification of poly(ADP-ribose) was established. Calf thymus nuclei were incubated with NAD. After pronase digestion, DNA was isolated as a flocculent form by adding 1 M NaCl and 2 volumes of ethanol. Acid-insoluble poly(ADP-ribose), which was synthesized during incubation, was quantitatively recovered in this DNA fraction. Poly(ADP-ribose) was purified as a single peak from a hydroxylapatite column after the nuclease treatment. Preparation of poly(ADP-ribose) thus obtained revealed the characteristics of pure poly(ADP-ribose), including a ratio of A280 to A260 of 0.24.
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000378:metabolism; D002845:Chromatography; D004247:DNA / Q000032:analysis; D006882:Hydroxyapatites; D009702:Nucleoside Diphosphate Sugars / Q000302:isolation & purification*; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis / Q000302:isolation & purification*; D011402:Pronase; D013950:Thymus Gland / Q000032:analysis / Q000378:metabolism
D006882:Hydroxyapatites; D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose; D004247:DNA; D011402:Pronase