A radioimmunoassay for mouse alpha-lactalbumin.
alpha-Lactalbumin has been purified from mouse milk by the use of ammonium sulfate precipitation, Bio-Gel P-100 chromatography and DEAE-cellulose chromatography. Mouse alpha-lactalbumin exists as two major charge forms with the same molecular weight. Both charge forms have been used in the development of a radioimmunoassay to mouse alpha-lactalbumin. The assay is specific for mouse alpha-lactalbumin, reacting identically with both forms, and sensitive with a minimal detectable concentration of 0.35 ng. alpha-Lactalbumin induced in mouse mammary gland explants has been measured with the use of the radioimmunoassay and is detectable in both the explants and media of the cultures.
D000818:Animals; D000918:Antibody Specificity; D046508:Culture Techniques; D007768:Lactalbumin / Q000032:analysis*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008892:Milk / Q000378:metabolism*; D011863:Radioimmunoassay / Q000379:methods
D007768:Lactalbumin