title: Ultrastructure of neurosecretosomes sedimented in the nuclear fraction from the posterior pituitary of the rat.
abstract: Subcellular fractions of the posterior pituitary of the rat were isolated by differential and density gradient centrifugation, and microsamples prepared for electron miscroscopy by KMnO4 or glutaraldehyde-OsO4 fixation. The nuclear fraction, P1 (1000 g x 7 min), was the main neurosecretosome (NSS) fraction and contained nuclei and mitochondria in addition to NSS (60%). The crude mitochondrial fraction, P2 (10,000 g x 20 min), contained free mitochondria (70%), NSS, unidentified membrane particles, fat droplets derived from pituicytes, neurosecretory granules (NSG) and synaptosomes, identified by the presence of synaptic membrane thickenings. Fraction P3 (32,900 g x 20 min) contained mainly free NSG, while fraction P4 (118,000 g x 30 min) had a high microsome content.
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D005260:Female; D008297:Male; D009411:Nerve Endings / Q000502:physiology / Q000648:ultrastructure; D009940:Organoids / Q000648:ultrastructure; D010904:Pituitary Gland, Posterior / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats; D013347:Subcellular Fractions / Q000502:physiology / Q000648:ultrastructure; D013574:Synaptosomes / Q000502:physiology / Q000648:ultrastructure