Semiconservative synthesis of single-stranded RNA by bacteriophage phi 6 RNA polymerase.
The RNA polymerase in the nucleocapsid of Pseudomonas phaseolicola bacteriophage phi 6 transcribed large, medium, and small single-stranded RNA from the viral double-stranded RNA genome by a semiconservative (displacement) mechanism. Approximately 23%, 63%, and 65% of the nucleocapsid particles in the assay mixture synthesized at least one round of large, medium, and small single-stranded RNA molecules, respectively. Some of these particles reinitiated synthesis such that an average of 1.5 large, 33 medium, and 24 small single-stranded RNAs were synthesized from each double-stranded RNA.
D001435:Bacteriophages / Q000201:enzymology / Q000235:genetics*; D002213:Capsid / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D011549:Pseudomonas; D012330:RNA, Double-Stranded / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis*; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases