title: Characterization of the exposed carbohydrates on the surface membrane of adult Schistosoma mansoni by analysis of lectin binding.
abstract: The surface architecture of adult male Schistosoma mansoni was explored using a range of lectins with differing carbohydrate specificites. Highest specific binding was achieved with concanavalin A and the agglutinin of molecular weight 60000 from Ricinus communis; the binding of wheat germ agglutinin was mostly non-specific. Small amounts of peanut agglutinin and soybean agglutinin binding were observed and the binding of these lectins was increased by pre-treating the parasite with neuraminidase. The fucose binding protein of Lotus tetragonolobus failed to bind. These results indicate that mannose and/or glucose, galactose, N-acetylglucosamine, N-acetyl-galactosamine and sialic acid are exposed on the surface of the adult male schistosome.
mesh_terms: D000116:Acetylgalactosamine / Q000032:analysis; D000117:Acetylglucosamine / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002462:Cell Membrane / Q000032:analysis; D003208:Concanavalin A / Q000378:metabolism; D005643:Fucose / Q000378:metabolism; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D037102:Lectins / Q000378:metabolism; D008297:Male; D008358:Mannose / Q000032:analysis; D012550:Schistosoma mansoni / Q000032:analysis*
chemical_list: D002241:Carbohydrates; D037102:Lectins; C027112:fucose-binding lectin; D003208:Concanavalin A; D005643:Fucose; D005947:Glucose; D000116:Acetylgalactosamine; D008358:Mannose; D000117:Acetylglucosamine; D005690:Galactose