Response to chemotherapy of EMT6 spheroids as measured by growth delay and cell survival.
Multicellular tumour spheroids of the EMT6 mouse tumour line have been grown in a static (i.e. non-spinner) culture system to a mean spheroid diameter of 250 mum. Samples of spheroids were then exposed for 1 h to graded concentrations of various cytotoxic drugs, and the response assayed by both growth delay and survival of clonogenic cells. For nitrogen mustard (HN2), melphalan, BCNU, CCNU and cis-platinum, a considerable recovery in measured cell survival was seen if correlation between growth delay and cell survival (measured at 24 h) was observed for these 5 agents. For adriamycin, actinomycin D and 5-fluorouracil, no increase in measured cell survival was seen for a 24h delay in assay, and these agents produced longer growth delays for a given level of cell kill.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002999:Clone Cells / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy*; D051379:Mice; D008954:Models, Biological; D013997:Time Factors
D000970:Antineoplastic Agents