[Measuring toxicity of metal salts to macrophages in spleen fragment test].
Studies were conducted with the view to finding out whether the spleen fragment test was suitable for measurement of toxicity of metal salts to macrophages. Inhibition of in vitro macrophage migration from spleen fragments of guinea pigs was tested as parameter of toxicity. The technique was simple and inexpensive. Those concentrations which inhibited macrophage migration by 50 per cent, as compared to controls, were interpolated from concentration-action relations given in a graph (EC50 values). The following order of toxicity was derived from those EC values, given in M: Na2SeO3 (1.5 x 10(-5)) > K2Cr2O7 (2.7 x 10(-5)) > CdCl2 (3.5 x 10(-5)) > HgCl2 (9.5 x 10(-5); > NiCl2 (2.8 X 10(-3)) > CrCl3 (4 x 10(-3)) > MnCl2 (1.3 x 10(-2)). Evidence was produced to antagonistic effects of both Na2SeO2 and HgCl2. The spleen fragment test, consequently, should enable also studies into in vitro interaction of metal salts.
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D046508:Culture Techniques; D006168:Guinea Pigs; D008264:Macrophages / Q000187:drug effects*; D008670:Metals / Q000633:toxicity*; D012492:Salts; D013154:Spleen / Q000166:cytology
D008670:Metals; D012492:Salts
Uber die Prüfung der Toxiziät von Metallsalzen auf Makrophagen im Milzfragmenttest.