[Influence of measurement time and reacton medium on kinetic immunturbidimetric protein determination (author's transl)].
For fixed-time rate analysis, the turbidity curve, which is the reference system of immunturbidimetric protein determination, depends on the time of first measurement, on the subsequent measurement time interval and on the composition of the reaction medium. The IgG/anti-IgG system has been chosen as an example to demonstrate how the reaction parameters can be optimized. In this context particular consideration has been given to some of the analyzers commonly used in larger laboratories (ABA-100, CentrifiChem, Cobas Bio, GEMSAEC, Gilford 3500).
D000937:Antigen-Antibody Reactions; D001322:Autoanalysis / Q000379:methods; D001798:Blood Proteins / Q000032:analysis*; D004121:Dimethyl Sulfoxide; D006801:Humans; D007118:Immunoassay; D007074:Immunoglobulin G / Q000032:analysis; D007700:Kinetics; D009391:Nephelometry and Turbidimetry; D011092:Polyethylene Glycols; D013997:Time Factors
D001798:Blood Proteins; D007074:Immunoglobulin G; D011092:Polyethylene Glycols; D004121:Dimethyl Sulfoxide
Der Einfluss von Messzeit und Reaktionsmilieu bei kinetischen immunturbidimetrischen Proteinbestimmungen.