[Carminomycin induction of single-stranded DNA breaks in the normal and tumorous cells of mice administered the antibiotic intravenously].
The effect of carminomycin administered intravenously to albino mice on the sedimentation character of the ascitic cell DNA of mouse lymphadenosis NK/Li, as well as the cells of the lungs, kidneys, liver, heart and spleen in alkaline sucrose gradients was studied. It was found that carminomycin in a dose of 3.5 mg/kg (LD50) induced DNA single-strand breaks in the cells of the lungs, kidneys, liver and heart. At the same time the single-strand breaks were not detected in the tumor cells NK/Li and the cells of the spleen. The results are indicative of correlation between sensitivity of the cells to carminomycin and a decreased capacity of the antibiotic for induction of single-strand breaks in DNA. Probably, the cell ability for DNA repair is an important factor defining the antitumor selective effect of carminomycin. Induction of the single-strand breaks reflects the role of the excision repair in healing the damages induced by carminomycin.
D000818:Animals; D002360:Carubicin / Q000627:therapeutic use*; D004260:DNA Repair / Q000187:drug effects; D004273:DNA, Neoplasm / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis*; D003630:Daunorubicin / Q000031:analogs & derivatives*; D004353:Drug Evaluation, Preclinical; D007275:Injections, Intravenous; D008223:Lymphoma / Q000188:drug therapy; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000188:drug therapy
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D002360:Carubicin; D003630:Daunorubicin
Issledovanie induktsii karminomitsinom odnonitevykh razryvov DNK v normal'nykh i opukholevykh kletkakh myshi pri vnutrevennom vvedenii antibiotika.