Induction of erythropoiesis with erythrocyte production in bone marrow cell culture "in vitro".
In the system of long-term bone-marrow culture described by Dexter et al., the differentiation of hematopoietic stem cells into erythropoietic cells is not observed. In this system, bone marrow are grown in a medium supplemented with horse serum. We describe here the induction of erythrocytic maturation in the bone-marrow culture by the addition of 5% fetal calf serum, one or two weeks after seeding the bone-marrow cells. We obtained the persistance up to 5 weeks of erythrocytic production. This phenomenon does not depend on the presence of erythropoietin in fetal calf serum.
D000818:Animals; D001854:Bone Marrow Cells; D002417:Cattle; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D004912:Erythrocytes / Q000166:cytology*; D004920:Erythropoiesis; D005312:Fetal Blood; D051379:Mice
D003470:Culture Media