Biological activity of 1,N6-ethenothiamin, a fluorescent analog of thiamin, in rats.
The ability of the recently synthesized fluorescent analog of thiamin, 1,N6-ethenothiamin, to substitute for thiamin in rats in vivo has been studied. 50 micrograms of ethenothiamin/100 g body weight/day injected in rats allowed about one-half the weight gain with 50 micrograms/100g/day of thiamin. The activities of the pyruvate and alpha-keto-glutarate dehydrogenases of liver mitochondria were partially restored by 50 micrograms/100g/day of ethenothiamin. Ethenothiamin at this level did not appear to restore the branched-chain alpha-keto acid dehydrogenase activities to any extent. When 150 micrograms of ethenothiamin/100g body weight/day was injected, growth was optimum, but these enzyme activities still did not achieve normal levels.
D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D000597:Amino Acids, Branched-Chain / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D007651:Keto Acids / Q000378:metabolism; D007658:Ketone Oxidoreductases / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000378:metabolism*; D051381:Rats; D013329:Structure-Activity Relationship; D013831:Thiamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013832:Thiamine Deficiency / Q000378:metabolism*
D000597:Amino Acids, Branched-Chain; D007651:Keto Acids; D009097:Multienzyme Complexes; C026806:ethenothiamin; D007658:Ketone Oxidoreductases; D042942:3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide); D013831:Thiamine