[Vital microscopy of the lung].
In order to develop an optimal method for prevital microscopy of the lung, in 12 experiments performed in mongrel dogs advantages of three methods of contact microscopy were compared: dark-field, polarization and fluorescent-absorptive. The results on investigating the lung microstructure and microcirculation demonstrated usefulness of all the three methods. Nevertheless, in order to estimate the lung microstructure and microcirculation, the dark-field method is the most expedient, to reveal the pleural vascular network, the best results are obtained with the fluorescent-absorptive microscopy. It is necessary to fix the part examined under the objective by means of a special fixator-sticker that does not traumatize the lung parenchyma and does not disturb the respiratory microcirculation.
D000818:Animals; D004285:Dogs; D008168:Lung / Q000033:anatomy & histology* / Q000098:blood supply; D008833:Microcirculation / Q000033:anatomy & histology; D008853:Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D008859:Microscopy, Polarization / Q000379:methods
Vital'naia mikroskopiia legkogo.