title: A critical temperature transition of K+-Na+ exchange in human lymphocytes.
abstract: Human lymphocytes were equilibrated for 48 hours at 5-6 mM K+ ex over a range of temperatures between 0 and 37 degrees C, and at 5 degrees C over a range of external K+ levels between 0 and 32 mM. Cell K+ and Na+ contents are normal between 37 and 10 decrees. Below 10 degrees there is a critical thermal transition in ion content centering around 3 degrees C. This and the steep sigmoidal isotherms of K+ and Na+ at 5 degrees C confirm the cooperative nature of ion exchange. At 0 degrees, cell K+ is maintained at a concentration that is seven to eight times that of the external medium. Isotopic K+ influx shows smaller, rapidly-exchanging, and larger, slowly-exchanging fractions. The latter, which correspond to the saturable, sigmoidal components of cell K+, are slowed by decreasing temperature. Although there is a critical temperature transition of K+-Na+ exchange, there is no corresponding transition for isotopic K+ exchange, which has an activation energy of 11.6 kcal/mole. The combined ion content and flux data are readily understood by reference to two major concepts of the association-induction hypothesis: that of rapid solute exclusion from cell water existing in a state of polarized multilayers, and that of solute accumulation limited by adsorption onto and desorption from fixed anionic sites that interact with one another in a critical, cooperative fashion.
mesh_terms: D000327:Adsorption; D002463:Cell Membrane Permeability; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013696:Temperature; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water; D012964:Sodium; D011188:Potassium