title: Adenylate cyclase activity of bovine spermatozoa during maturation in the epididymis and the activation of sperm particulate adenylate cyclase by GTP and polyamines.
abstract: The adenylate cyclase activity of bovine caput and cauda epididymal spermatozoa was measured in intact- and in broken-cell preparations. Cyclase activity was 4-fold greater in caput than in cauda cells and total cyclase activity in broken-cell preparations was 3-5 times greater than that in intact cells. A particulate fraction derived from sonically disrupted caput spermatozoa was used to study the effects of compounds that might be physiologically important modulators of adenylate cyclase. Activity was stimulated by GTP, 5-guanylyl imidophosphate and by the polyamines, spermine and spermidine.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004789:Enzyme Activation / Q000187:drug effects; D004822:Epididymis; D006160:Guanosine Triphosphate / Q000494:pharmacology*; D006165:Guanylyl Imidodiphosphate / Q000494:pharmacology; D008297:Male; D011073:Polyamines / Q000494:pharmacology*; D013079:Sperm Maturation; D013094:Spermatozoa / Q000201:enzymology*; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology
chemical_list: D011073:Polyamines; D013096:Spermine; D006165:Guanylyl Imidodiphosphate; D006160:Guanosine Triphosphate; D000262:Adenylyl Cyclases; D013095:Spermidine