A potential DNA replication complex isolated from sea urchin embryos by renografin gradient centrifugation.
A complex containing deoxyribonucleic acid, protein and lipid has been isolated from the nuclei of the sea urchin Stronglocentrotus pupuratus by gentle lysis of nuclei and centrifugation of the lysate in a renografin gradient. The complex is similar in several respects to DNA-membrane complexes isolated by the sarkosyl M-band technique [1]. These include the following: 1, most of the cellular DNA is present in the complex but the bulk of the DNA may be removed by sonication or nuclease treatment; 2, nascent DNA is preferentially found in the complex; and 3, destruction of membrane/protein components removes DNA found in the complex.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D004261:DNA Replication; D003974:Diatrizoate Meglumine; D004625:Embryo, Nonmammalian / Q000378:metabolism; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009685:Nuclear Envelope / Q000378:metabolism; D012617:Sea Urchins / Q000378:metabolism*
D008563:Membrane Lipids; D008565:Membrane Proteins; D003974:Diatrizoate Meglumine