title: Adjuvant and immunosuppressive effects of retinol and Tween 80 on IgG production in mice.
abstract: The effect of retinol emulsified in Tween 80, alone or in combination with Al(OH)3, on the adjuvant-independent and dependent IgG responses to ovalbumin (OA) were examined by passive hemagglutination and passive cutaneous anaphylaxis, respectively. High antibody levels were detected after a second injection of OA on day 30, however, primary IgG antibodies were either partially (adjuvant independent) or totally (adjuvant dependent) suppressed. Animals treated with saline:Tween 80 prior to immunization with OA in saline as a control produced no detectable antibody response after either antigen injection. Additional control animals pretreated with saline:Tween 80 and then immunized with OA absorbed to Al(OH)3 [OA:Al(OH)3] showed suppressed primary responses and a significant increase in antibody levels after a second injection of OA:Al(OH)3. These results indicate that although th retinol component of retinol:Tween 80 caused an immunopotentiation, the Tween 80 component effected a temporary suppression.
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000536:Aluminum Hydroxide / Q000494:pharmacology; D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D006386:Hemagglutination Tests; D007074:Immunoglobulin G / Q000096:biosynthesis; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D011136:Polysorbates / Q000494:pharmacology*; D014801:Vitamin A / Q000494:pharmacology*
chemical_list: D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D007166:Immunosuppressive Agents; D011136:Polysorbates; D014801:Vitamin A; D000536:Aluminum Hydroxide; D010047:Ovalbumin