Intravesical prostaglandin: release and effect of bladder instillation on some micturition parameters.
Bladder washout effluent fluid was examined for prostaglandin-like activity following acidic lipid extraction and bioassay using the rat-stomach strips technique. A low basal level of prostaglandin-like material was detected in washouts collected when the bladder was empty in 4 out of 7 dogs. Increased levels were detected following micturition in 5 of these 7 dogs. A significantly high level of prostaglandin-like material was recovered after pelvic nerve stimulation. The prostaglandin-like material was tentatively identified as PGE based on the bioassay with the addition of specific antibody. Intravesically administered PGE2 or PFG2 alpha significantly reduced, in a dose-dependent response, the threshold micturition volume. All of these findings suggested that prostaglandins may play a role in the micturition process.
D000818:Animals; D001826:Body Fluids / Q000032:analysis; D004106:Dilatation; D004285:Dogs; D005260:Female; D011453:Prostaglandins / Q000378:metabolism / Q000502:physiology*; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D001743:Urinary Bladder / Q000294:innervation / Q000378:metabolism / Q000502:physiology; D014554:Urination / Q000187:drug effects
D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F