Normal immunosuppressive protein. Isolation of a glycoprotein active fraction.
Experiments to determine the nature of the active moiety of Normal immunosuppressive protein (Nip) were performed using Sepharose Con A fractionation. Nip was found to be a glycoprotein or a glycopeptide. The fraction eluted by 0.2 M mannose was found to be as active as the crude Nip preparation in inhibiting EL4 tumour cell proliferation in vitro. It also gave a positive haemagglutination inhibition test when added to a rabbit anti-Nip antibody system. The resistance of Nip to boiling as well as the positive PAS staining also confirmed the glycoprotein or glycopeptide nature of Nip.
D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006020:Glycopeptides / Q000276:immunology; D006023:Glycoproteins / Q000097:blood / Q000276:immunology* / Q000302:isolation & purification; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007108:Immune Tolerance; D007166:Immunosuppressive Agents; D066298:In Vitro Techniques; D008223:Lymphoma / Q000276:immunology; D009374:Neoplasms, Experimental / Q000276:immunology; D011247:Pregnancy
D006020:Glycopeptides; D006023:Glycoproteins; D007166:Immunosuppressive Agents