Determination of polycyclic aromatic hydrocarbons in fat products by high pressure liquid chromatography.
The method previously described by Gertz [1] for the extraction of 3,4-benzopyrene in meat- and fishproducts has been adapted for butter, margarine, edible oils and fats and extended to other polycyclic aromatic hydrocarbons (PAH): 1,2-benzopyrene, 1,2,3,4-dibenzanthracene, 1,2,5,6-dibenzanthracene and 1,12-benzoperylene. The isolated PAH are determined by high-pressure liquid chromatography on reversed-phase columns with detection by fluorimetry. Recoveries range from 76 to 85%. The detection limit varies between 0.10 and 0.90 ppb. By application of optimized analysis conditions the sensitivity can be increased to 0.01 ppb for each compound.
D001551:Benz(a)Anthracenes / Q000032:analysis; D001580:Benzopyrenes / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005223:Fats / Q000032:analysis*; D009821:Oils / Q000032:analysis; D010569:Perylene / Q000031:analogs & derivatives / Q000032:analysis; D011083:Polycyclic Compounds / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D001551:Benz(a)Anthracenes; D001580:Benzopyrenes; D005223:Fats; D009821:Oils; D011083:Polycyclic Compounds; C006710:benzotriphenylene; D010569:Perylene; C006718:1,12-benzoperylene; C026487:benzo(e)pyrene; C026486:1,2,5,6-dibenzanthracene