Frequency distribution of pre-messenger RNA sequences in polyadenylated and non-polyadenylated nuclear RNA from Friend cells.
Hybridisation of cDNA probes for abundant and rare polysomal polyadenylated RNAs with polyadenylated and non-polyadenylated nuclear RNA from Friend cells indicated that the abundant polysomal polyadenylated RNA sequences were present at a higher concentration in the nucleus than rare polysomal sequences, but at a reduced range of concentrations. The ratio of the concentrations of abundant and rare sequences was about 3 in non-polyadenylated nuclear RNA, 9 in polyadenylated nuclear RNA and 13 in polysomal polyadenylated RNA. This suggests that polyadenylation may play a role in the quantitative selection of sequences for transport to the cytoplasm. Polyadenylation cannot be the only signal for transport, since a highly complex population of nucleus-confined polyadenylated molecules exists, each of which is present on average at less than one copy per cell.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D007700:Kinetics; D007942:Leukemia, Experimental; D051379:Mice; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000096:biosynthesis*; D011132:Polyribosomes / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D014158:Transcription, Genetic
D012333:RNA, Messenger; D011061:Poly A