Isolation of human NK cells by density gradient centrifugation.
Sedimentation characteristics of human NK cells in discontinuous Percoll density gradients were studied. NK activity against the leukaemic cell line K-562 peaked in a single low density fraction mainly consisting of large granular lymphocytes previously shown to be the principal NK cells in human peripheral blood. Percoll density gradient centrifugation provides a useful tool for analysis of human NK cells and their relationship to other blood mononuclear cells.
D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D003602:Cytotoxicity, Immunologic; D007694:Killer Cells, Natural / Q000166:cytology* / Q000276:immunology; D008211:Lymphocyte Cooperation; D008212:Lymphocyte Depletion