A simple method for the recovery of active oligomeric enzyme from its diluted solutions.
A method for the recovery of active enzyme from its diluted solutions is described. It includes batch sorption on DEAE-Sephadex A-50 which follows elution or chromatography of an enzyme on a DEAE-Sephasex A-50 column. All purified aminoacyl-tRNA synthetases retain high activity without degradation of the protein. The method is very fast and convenient.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D001126:Arginine-tRNA Ligase / Q000302:isolation & purification; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D008718:Methionine-tRNA Ligase / Q000302:isolation & purification; D010652:Phenylalanine-tRNA Ligase / Q000302:isolation & purification; D012639:Seeds / Q000201:enzymology*
D000604:Amino Acyl-tRNA Synthetases; D008718:Methionine-tRNA Ligase; D001126:Arginine-tRNA Ligase; D010652:Phenylalanine-tRNA Ligase