Neutrophil chemotaxis in the presence of antibiotics: a re-evaluation using an agarose technique.
Neutrophil chemotaxis in the presence of tetracycline and clindamycin was evaluated using an agarose technique. No significant inhibition of chemotaxis by these antibiotics could be demonstrated, in contrast to previously reported findings using the Boyden chamber technique for measurement of chemotaxis. Both agents caused a minor increase in movement towards a P. acnes derived chemotactic factor. Some reduction of chemokinesis by high concentrations of tetracycline was noted. No effect of the drugs upon generation of chemotactic activity from whole serum could be demonstrated. Normal cell movement occurred in the absence of extracellular calcium and magnesium, in contrast to reported findings using the Boyden chamber.
D000328:Adult; D002413:Cations, Divalent / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D002981:Clindamycin / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D009504:Neutrophils / Q000502:physiology; D012685:Sepharose; D013752:Tetracycline / Q000494:pharmacology*; D015054:Zymosan
D002413:Cations, Divalent; D002981:Clindamycin; D015054:Zymosan; D012685:Sepharose; D013752:Tetracycline