title: Incorporation of influenza virus M-protein into liposomes.
abstract: M-protein from influenza virus vaccine was purified by sodium dodecyl sulfate-gel chromatography and incorporated into liposomes by solubilization with octylglucoside and subsequent dialysis. Liposomes containing M-protein formed a distinct population with a density of 1.22 g/ml on sucrose-gradient centrifugation, regardless of the net charge on the liposomes. Treatment of the liposomes by freeze-fracture followed by electron microscopic examination showed multilamellar structures in those liposomes without M-protein; liposomes containing M-protein were mulberry-like structures which appeared unilamellar. These studies show incorporation of M-protein into the lipid bilayer.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D005614:Freeze Fracturing; D009980:Influenza A virus / Q000032:analysis*; D008051:Lipid Bilayers; D008081:Liposomes; D008854:Microscopy, Electron; D014764:Viral Proteins
chemical_list: D008051:Lipid Bilayers; D008081:Liposomes; D014764:Viral Proteins