Human monoclonal antibody against Forssman antigen.
Hybrid cells formed between human lymphocytes and mouse myeloma cells produce human immunoglobulin in culture. Stable antibody-producing cell lines can be isolated after multiple cycles of low-density passage, cloning, and continued selection for immunoglobulin production. The origin and characteristics of a hybrid of human and mouse cells is described. This hybrid produces high concentrations (8.3 micrograms per milliliter) of human immunoglobulin M reactive with the terminal disaccharide of the Forssman glycolipid. These findings point to the potential use of human-mouse hybrid cells as a source of human monoclonal antibodies for therapeutic and diagnostic purposes.
D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D000918:Antibody Specificity; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D005577:Forssman Antigen; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis; D051379:Mice
D000906:Antibodies; D007075:Immunoglobulin M; D005577:Forssman Antigen