[Dissociation and decompactization of chromatin by heparin in a medium of "physiologic" ionic strength].
It has been shown for the first time that heparin, one of the natural polyanions, is capable of dissociating the histones H1, H2A and H2B from chromatin in a medium of "physiological" ionic strength: 0.15 M NaCl + 0.7 mM Na-phosphate buffer, pH 7.0. Under the same conditions heparin produces chromatin fractionation, leading to the appearance of slow-sedimenting DNA, augments viscosity and reduces turbidity of chromatin suspension. This evidences the decompactization of deoxyribonucleoprotein fibrils.
D000818:Animals; D002417:Cattle; D002498:Centrifugation; D002843:Chromatin / Q000187:drug effects*; D004247:DNA / Q000032:analysis; D003852:Deoxyribonucleoproteins / Q000032:analysis; D006493:Heparin / Q000494:pharmacology*; D009698:Nucleoproteins / Q000032:analysis; D009994:Osmolar Concentration; D011487:Protein Conformation / Q000187:drug effects; D013950:Thymus Gland / Q000032:analysis; D014783:Viscosity
D002843:Chromatin; D003852:Deoxyribonucleoproteins; D009698:Nucleoproteins; D006493:Heparin; D004247:DNA
Dissotsiatsiia i dekompaktizatsiia khromatina geparinom v srede "fiziologicheskoĭ"ionnoiĭ sily.