Hepatitis B surface antigen polypeptides: artifactual bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis caused by aggregation.
Hepatitis B surface antigen, subtype ad, was purified and studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions. Two major bands with molecular weights of 23,500 and 27,500 and several weaker bands with higher molecular weights were observed. When the low-molecular-weight bands and the group of high-molecular-weight bands were excised from the gel, eluted, and reelectrophoresed, neither the low-molecular-weight bands nor the high-molecular-weight bands ever appeared alone, but both high- and low-molecular-weight bands always appeared. It was concluded that the apparently high-molecular-weight bands represented aggregates of the two small polypeptides whose monomers formed the major bands. The preparation thus contained only two polypeptides.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D008623:Mercaptoethanol / Q000494:pharmacology; D008970:Molecular Weight; D011487:Protein Conformation; D014508:Urea / Q000494:pharmacology
D006514:Hepatitis B Surface Antigens; D008623:Mercaptoethanol; D014508:Urea