Purification of clofibrate-induced carnitine acetyltransferase from rat liver mitochondria.
The enzyme carnitine acetyltransferase (acetyl-CoA:carnitine O-acetyltransferase, EC 2.3.1.7) has been purified to homogeneity from hepatic mitochondria of clofibrate-fed rats. It is a protein of molecular weight 56 000 composed of two non-identical subunits of molecular weight 34 000 and 25 000. The enzyme is inhibited by palmityl-CoA as well as acetyl carnitine. The inhibition by fatty acyl-CoA is competitive with respect to both the substrates, carnitine and acetyl-CoA. The inhibition by acetylcarnitine is reversed by carnitine but not by acetyl-CoA.
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000108:Acetylcarnitine / Q000494:pharmacology; D000123:Acetyltransferases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D000214:Acyl Coenzyme A / Q000494:pharmacology; D000818:Animals; D002331:Carnitine / Q000378:metabolism; D002994:Clofibrate / Q000494:pharmacology*; D004790:Enzyme Induction; D008297:Male; D008930:Mitochondria, Liver / Q000201:enzymology*; D010171:Palmitoyl Coenzyme A / Q000494:pharmacology; D051381:Rats; D013696:Temperature
D000214:Acyl Coenzyme A; D010171:Palmitoyl Coenzyme A; D000108:Acetylcarnitine; D000105:Acetyl Coenzyme A; D000123:Acetyltransferases; D002994:Clofibrate; D002331:Carnitine