Serum acetaminophen assay using activated charcoal adsorption and gas chromatography without derivatization.
A quantitative assay of acetaminophen in serum has been developed. The drug, together with an internal standard 2-acetamidophenol, is adsorbed on activated charcoal and then extracted into a mixture of ethyl acetate and isopropanol. This extract is then analyzed, without any derivatization, by gas chromatography. The isothermal analysis yielded a good, highly reproducible separation. The drug peak was symmetrical and without any tailing. The peak height response ratio was found to be linear with concentrations ranging from 25-500 ng/L. No interference was observed with the various drugs or metabolites which are commonly encountered in human serum.
D000082:Acetaminophen / Q000097:blood*; D000327:Adsorption; D002606:Charcoal; D002849:Chromatography, Gas / Q000379:methods*; D006801:Humans
D002606:Charcoal; D000082:Acetaminophen