[Physical mapping of Streptomyces coelicolor Ae(2) actinophages. IV. Partial denaturation maps].
By measuring temperature transition profiles evidence was obtained that DNA of actinophage phiC31 has a random base distribution and the content of GC pairs is 63%. The denaturation maps of the DNA molecules of actinophages phiC31c28, phiC43del and phiC62 are similar and characteristic of these random base distributions. Main peaks are located at positions: 0.03, 0.25, 0.33, 0.53 and 0.99 from the left end (in relative units). Positions of the regions deleted in phages phiC31c28 and phiC43del were established in respect to phage phiC62 genome and shown not to coincide with the position of the denaturation peaks on the phiC62 DNA map. It seems unlikely that AT rich regions are preferrable ones for deletions in actinophage DNAs.
D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis*; D005814:Genes, Viral; D006358:Hot Temperature; D009691:Nucleic Acid Denaturation; D009711:Nucleotides / Q000032:analysis; D013302:Streptomyces
D004279:DNA, Viral; D009711:Nucleotides
Fizicheskoe kartirovanie aktinofagov Streptomyces coelicolor A3(2). IV. Karty chastichnoĭ denaturatsii.