Effects of adrenaline-tablets on rat heart cells in tissue culture at normal and raised temperature.
Primary cultures of rat heart cells were incubated at 37 degrees C or 39.5 degrees C. For permanent treatment with adrenaline recently developed tablets were added to the cultures. After 10 hours the cell counts, glucose, lactate, LDH, alpha-HBDH and GOT were determined. Permanent adrenaline-application led to a decrease of cell counts, an increase of lactate, LDH, alpha-HBDH and GOT. The results indicate an injured membrane function of rat heart cells. Raised temperature sensitized the cells for adrenaline-treatment.
D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D004837:Epinephrine / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D007773:Lactates / Q000378:metabolism; D009206:Myocardium / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D013696:Temperature
D007773:Lactates; D005947:Glucose; D004837:Epinephrine