Rapid sodium dodecyl sulfat/polyacrylamide gel electrophoresis of urinary proteins.
We describe a procedure for the convenient separation of proteins by sodium dodecyl sulfate/polyacrylamide gel electrophoresis of urine from cases of renal disease. A precipitation method that requires no special apparatus was used to concentrate the urinary proteins; for electrophoretic separation we used a commercially supplied polyacrylamide/cellulose gel slab. This method seems to be valuable for investigation of proteinuria; we recommend it for routine use.
D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007460:Iodoacetamide; D011507:Proteinuria / Q000652:urine*; D012967:Sodium Dodecyl Sulfate; D014462:Ultrafiltration
D012967:Sodium Dodecyl Sulfate; D007460:Iodoacetamide