[Effect of steroid hormones on the stability of isolated lysosomes during freezing].
Effect of hydrocortisone and dexamethasone, combined with cryoprotector polyethylene oxide, on stability of lysosomal membranes was studied after freezing of the lysosomes using various conditions. In freezing of the lysosomal suspension together with the cryoprotector and the steroid hormones numerous enzymes were liberated from the organelles less distinctly as compared with the control experiments without hormones. The steroid hormones stabilized apparently the phospholipid membrane of lysosomes under conditions of freezing. The steroid compounds might be used as stabilizing adducts in cryoconservation of tissues.
D000818:Animals; D003451:Cryoprotective Agents / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005615:Freezing; D006854:Hydrocortisone / Q000494:pharmacology*; D007425:Intracellular Membranes / Q000187:drug effects; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D051381:Rats
D003451:Cryoprotective Agents; D003907:Dexamethasone; D006854:Hydrocortisone
Vliianie steroidnykh gormonov na stabil'nost' izolirovannykh lizosom v usloviiakh zamorazhivaniia.