The effect of plasticizers of plastic material bags on biochemical and morphological changes of morphotic blood elements.
The concentration of di-2-ethylhexylphthalate (DEHP) was determined in whole blood, packed red cells and plasma of the blood collected into blood plastic bags manufactured by McGaw, Biotest or Polfa, in samples withdrawn immediately after collection and 1, 2 and 3 weeks later. ATPase activity and electrolyte (K+, Na+, Mg++) composition of erythrocytes were tested and possible morphological changes were inspected in phase-contrast microscope. Platelet aggregation induced by ADP, collagen and adrenaline was also investigated. Blood stored in glass bottles served as a standard. The DEPH concentrations were similar in samples contained in various bags. Maximum DEPH concentration in the whole blood, after 3 weeks of storage, attained 52 microgram/cm3. ATP-ase activity was more rapidly depressed in the blood kept in bags instead of glass bottles. Erythrocyte morphology and results of platelet function tests remained unaffected.
D000328:Adult; D001793:Blood Preservation / Q000295:instrumentation*; D001803:Blood Transfusion; D004051:Diethylhexyl Phthalate / Q000097:blood / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D010795:Phthalic Acids / Q000633:toxicity*; D010969:Plastics / Q000633:toxicity
D010795:Phthalic Acids; D010969:Plastics; D004051:Diethylhexyl Phthalate