Analysis of 4'-demethylepipodophyllotoxin-9-(4,6-O-ethylidene-beta-D-glucopyranoside) by high-pressure liquid chromatography.
A rapid and specific high-pressure liquid chromatographic assay is described for the quantitative analysis of 4'-demethylepipodophyllotoxin-9-(4,6-O-ethylidene-beta-D-glucopyranoside) (I) in plasma. After chloroform extraction, I was analyzed by reversed-phase chromatography and UV detection (252nm). The maximum sensitivity was 0.1 microgram/ml. Quantitation was by relative response factor calibration using an integrating microcomputer. Over the concentration range of 0.5-90 microgram/ml, the average recovery of I from plasma was 95.4 +/- 3.8% (SD).
D000970:Antineoplastic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005047:Etoposide / Q000097:blood*; D006801:Humans; D011034:Podophyllotoxin / Q000031:analogs & derivatives*
D000970:Antineoplastic Agents; D005047:Etoposide; D011034:Podophyllotoxin