Measurement of a stimulatory protein of RNA polymerase II in various mouse organs by the complement fixation test.
The amount of S-II, a protein specifically stimulating RNA polymerase II, was measured in various mouse organs by a micro complement fixation assay. The amount was almost the same in brain, liver, kidney, spleen, and Ehrlich ascites tumor cells on the basis of DNA, being about 3.8 micrograms/mg DNA, which corresponds to 3.6 X 10(5) molecules/cell. However, the amount of S-II decreased greatly during erythro-differentiation of Friend leukemia cells and no S-II was detected matured erythrocytes.
D000818:Animals; D001923:Brain Chemistry; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D002460:Cell Line; D003168:Complement Fixation Tests; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004789:Enzyme Activation; D004912:Erythrocytes / Q000032:analysis; D007668:Kidney / Q000032:analysis; D007942:Leukemia, Experimental / Q000032:analysis; D008099:Liver / Q000032:analysis; D008297:Male; D051379:Mice; D009363:Neoplasm Proteins / Q000032:analysis*; D012319:RNA Polymerase II / Q000378:metabolism*; D013154:Spleen / Q000032:analysis; D014157:Transcription Factors / Q000032:analysis*; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors
D009363:Neoplasm Proteins; D014157:Transcription Factors; D035165:Transcription Factors, General; D035602:Transcriptional Elongation Factors; C018648:transcription factor S-II; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases