Characterization of the concanavalin A-induced increase in lymphocyte cell membrane permeability by furosemide.
The effect of ConA on passive 42K+ fluxes in human lymphocytes was analysed by treatment with furosemide and ouabain. Furosemide has no effect on net K+ transport and pump flux. The component of passive efflux of 42K+ which depends on K+-K+ exchange is inhibited by furosemide; its mechanism is discussed in detail. The 42K+ uptake in lymphocytes consists of 1. an ouabain-sensitive pump influx, 2. an ouabain-insensitive leak influx and 3. a furosemide-sensitive K+-K+-exchange. Only the first two components of 42K+ uptake are stimulated by ConA.
D001692:Biological Transport / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003208:Concanavalin A / Q000494:pharmacology*; D005665:Furosemide / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000502:physiology*; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism
D003208:Concanavalin A; D010042:Ouabain; D005665:Furosemide; D011188:Potassium