High-performance liquid chromatography of myosin's subfragment 1 and light chains on Spherogel TSK-type columns.
Myosin's subfragment 1 and three light chains were chromatographed rapidly at room temperature on Spherogel TSK-type columns, recently developed for steric-exclusion high-performance liquid chromatography, with no adsorption of the proteins to the columns or loss of enzymic activity. Crude preparations of subfragment 1 were extensively purified on Spherogel TSK SW-3000 columns. Unresolved alpha chymotryptic-subfragment 1 was separated into its two isoenzymes despite only a small difference in molecular weights, presumably because of conformational differences. Light chain 2 was purified from a mixture of all three light chains by chromatography on a Spherogel TSK SW-2000 column; light chains 1 and 3 eluted as two distinct complexes having ratios of 2:1 and 1:1.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009218:Myosins / Q000032:analysis*; D011498:Protein Precursors / Q000032:analysis*; D011817:Rabbits
D011498:Protein Precursors; D009218:Myosins