[Role of different fractions of adhering splenocytes in inducing antibody formation in irradiated mice].
Adhering splenic cells were separated into the following fractions by centrifugation in the Ficoll density gradient: A (12--16% of Ficoll in the interphase), B (16--21%), C (21--25%) and D (in the sediment). The influence of the cells in fractions B, C and D on the immune response to sheep red blood cells was studied in vivo (in sublethally irradiated mice). When injected simultaneously with lymphocytes, the splenic cells in fractions B and C significantly increased the number of antibody-producing cells in the recipient's spleen. Without lymphocytes neither the initial population of adhering splenic cells, nor its fractions induced the production of antibodies.
D000818:Animals; D000917:Antibody Formation; D001202:Ascitic Fluid / Q000166:cytology; D002448:Cell Adhesion; D002452:Cell Count; D004912:Erythrocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D011828:Radiation Chimera; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology* / Q000637:transplantation; D014184:Transplantation, Homologous
Rol' raznykh fraktsiĭ prilipaiushchikh kletok selezenki v induktsii anti teloobrazovaniia u obluchennykh mysheĭ.