Solid-phase synthesis of polynucleotides. II. Synthesis of polythymidylic acids by the block coupling phosphotriester method.
Synthesis of two oligothymidylic acids, tridecamer and nonadecamer, is described by a rapid and simple solid-phase method on two kinds of polyacrylamide supports derivatized from commercially available Enzacryl Gel K-2. The syntheses were performed by the phosphotriester method using di- and tri-thymidylic acid blocks as the incoming 3'-phosphodiester component. High coupling yields were consistently obtained and the final product was isolated very easily by high performance liquid chromatography on Permaphase AAX.
D007202:Indicators and Reagents; D008722:Methods; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011071:Poly T / Q000138:chemical synthesis*; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis*; D012116:Resins, Plant; D013053:Spectrophotometry; D013936:Thymidine / Q000031:analogs & derivatives
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D011089:Polydeoxyribonucleotides; D012116:Resins, Plant; D011071:Poly T; C028273:5'-O-dimethyltritylthymidine; D013936:Thymidine