Physicochemical measurement of the base composition of mRNA-related sequences of the human alpha and beta globin genes.
Hybrids formed between human alpha and beta globin cDNA and total human cellular DNA have been studied by thermal denaturation and cesium chloride density gradient centrifugation. From these studies, the weight average G + C content of human alpha globin cDNA has been determined to be 62% +/- 2% and that of human beta globin cDNA 51% +/- 2%. These values correlate well with the results of G + C content of the human alpha and beta globin cDNAs as determined by direct nucleotide sequence analysis of the cDNAs. Thermal denaturation and cesium chloride density gradient centrifugation of DNA-cDNA hybrids can therefore provide accurate information on the base composition of mRNA related sequences of any single copy gene for which a relatively pure cDNA can be obtained, without the necessity for direct nucleotide sequence analysis.
D001482:Base Composition; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000032:analysis*; D006441:Hemoglobin A / Q000235:genetics; D006801:Humans; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D010446:Peptide Fragments / Q000235:genetics; D012333:RNA, Messenger
D010446:Peptide Fragments; D012333:RNA, Messenger; D004247:DNA; D006441:Hemoglobin A