A possible role of rabbit heart cytosol tocopherol binding in the transfer of tocopherol into nuclei.
An alpha-tocopherol-binding macromolecule was isolated from the heart cytosol of rabbits fed for 1 month with an alpha-tocopherol-deficient diet. The amount of [3H]-tocopherol bound to nuclear chromatin was increased when the alpha-tocopherol-deficient heart nuclei were incubated in the presence of [3H]tocopherol-cytosol complex. In this condition, large amounts of [3H]tocopherol were associated with a subnuclear fraction that contained non-histone acidic proteins.
D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D011817:Rabbits; D014810:Vitamin E / Q000302:isolation & purification / Q000378:metabolism*; D014811:Vitamin E Deficiency / Q000378:metabolism
D002352:Carrier Proteins; C017763:alpha-tocopherol transfer protein; D014810:Vitamin E