Mouse complement components C3 and C4. Characterization of their messenger RNA and molecular cloning of complementary DNA for C3.
Mouse liver mRNA species which direct the cell-free synthesis of pro-C3 and pro-C4 polypeptides with an apparent molecular weight of 175,000 and 190,000, respectively, were shown to sediment faster than 28 S. By electrophoresis in a denaturing gel the mRNA for C3 was determined to contain approximately 7,500 ribonucleotides. cDNA was synthesized from size-fractionated mouse liver mRNA and cloned in the plasmid pBR 322. Among the cDNA clones recovered three were identified as being complementary to portions of the mRNA for C3.
D000818:Animals; D001482:Base Composition; D003001:Cloning, Molecular; D003176:Complement C3 / Q000235:genetics*; D003181:Complement C4 / Q000235:genetics*; D004274:DNA, Recombinant / Q000378:metabolism*; D005796:Genes; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*
D003176:Complement C3; D003181:Complement C4; D004274:DNA, Recombinant; D012333:RNA, Messenger