title: Trypanosoma brucei: the effect of glycerol on the anaerobic metabolism of glucose.
abstract: Studies measuring the glycolytic intermediate and adenine nucleotide concentrations in Trypanosoma brucei metabolising glucose either aerobically or under conditions where glycerol-3-phosphate oxidase is inactive have shown the following: 1. Inhibition with 0.5 mM salicylhydroxamic acid (SHAM) accurately simulates anaerobic conditions in T. brucei; 2. On inhibition of respiring cells with 0.5 mM SHAM, the concentrations of most glycolytic intermediates decrease; they decrease further as the concentration of glycerol, an end product, increases. Only the concentration of sn-glycerol-3-phosphate is increased. This increase depends upon the method of preparation but is independent of time and glycerol concentration. 3. Glycerol formation from sn-glycerol-3-phosphate is coupled to the phosphorylation of another compound. The results of these studies are consistent with this compound being ADP; 4, The degree of inhibition of the anaerobic metabolism of glucose exerted by glycerol varies with the sn-glycerol-3-phosphate concentration, implying that the effect of glycerol is at the site of sn-glycerol-3-phosphate: ADP transphosphorylation.
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000693:Anaerobiosis; D000818:Animals; D005947:Glucose / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism; D006019:Glycolysis; D011773:Pyruvates / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000378:metabolism*
chemical_list: D000227:Adenine Nucleotides; D005994:Glycerophosphates; D011773:Pyruvates; D005947:Glucose; D005990:Glycerol