Determination of duodenogastric reflux by use of 14C-labelled cholic acid. An experimental study in dogs.
A method for determination of duodenogastric reflux was elaborated in animal experiments. The bile acid pool was labelled by intravenous injection of 14C-cholic acid and the activity in consecutively collected gastric samples was determined. The gastric samples were taken approximately 24 hours after injection of the tracer. In one dog, provided with a continent duodenostomy, the radioactivity in duodenal samples was found to vary moderately 24-32 hours after injection of labelled cholic acid. The method was tested in three different groups of dogs: a) "normal dogs", B) dogs having undergone partial gastric resection (B I), and C) dogs provided with a reflux-preventing valve after partial gastric resection. Radioactivity was measured in gastric samples from one out of 15 normal dogs and in samples from 11 out of 13 dogs after partial gastric resection. After interposition of a reflux-preventing valve, no radioactivity was found in samples from six tested dogs. Measurement of radioactivity in gastric samples collected approximately 24 hours after labelling of the bile acid pool seems to be a reliable and simple method for semi-quantitative determination of duodenogastric reflux.
D000818:Animals; D001655:Bile Reflux / Q000175:diagnosis*; D001660:Biliary Tract Diseases / Q000175:diagnosis*; D002250:Carbon Radioisotopes; D002793:Cholic Acids; D004285:Dogs; D004378:Duodenal Diseases / Q000175:diagnosis*; D004386:Duodenum / Q000601:surgery; D005743:Gastrectomy; D005750:Gastric Juice / Q000032:analysis; D013272:Stomach Diseases / Q000175:diagnosis*
D002250:Carbon Radioisotopes; D002793:Cholic Acids