Identification of deoxy-alpha-tocopherolquinol as another endogenous electron donor for biohydrogenation.
Solvent extracts of Butyrivibrio fibrisolvens contain 2-[3, 7, 11, 15-tetramethylhexadecyl]-3, 5, 6-trimethyl-benzoquinol (deoxy-alpha-tocopherolquinol) that can serve as an alternate electron donor for alpha-tocopherolquinol for the biohydrogenation of cis-9, trans-11-octadecadienoate. In addition, the cell extracts contain deoxy-alpha-tocopherolquinone. This compound arises metabolically from alpha-tocopherolquinone via dehydration to trimethylphytylbenzoquinone followed by hydrogenation to deoxy-alpha-tocopherolquinone. Although the hydrogenation of the isoprene double bond and the conjugated fatty acid both use NADH as the primary reductant, the two reactions appear to be catalyzed by different enzymes.
D004579:Electron Transport; D006089:Gram-Negative Anaerobic Bacteria / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
C027692:deoxy-alpha-tocopherolquinol; D014810:Vitamin E; D024502:alpha-Tocopherol