title: Proliferation of human lymphoid cells. Lymphocytopoiesis and cell cycle parameters in isolated perfused human spleens.
abstract: Parameters of the cell cycle of lymphoid cells and the rate of production of small lymphocytes and mature plasma cells were estimated in human spleens. 12 normal and 1 enlarged spleen with lymphatic hyperplasia were labelled either by a pulse or continuously with 3H-TdR-thymidine during normothermic perfusion of the isolated spleens. Biopsies of splenic tissue were taken at different intervals and evaluated autoradiographically. The mean initial labelling index of immunoblasts was 73% and for plasmoblasts 52%. The labelling index for small lymphocytes and mature plasma cells increased initially from 0% to 1%, and up to 25% respectively after 14 h. The duration of the S-phase, as determined by double labelling, was 8.26 h for lymphoid blasts. Data from the percentage labelled mitosis curve resulted in a minimal tG2 of about 1 h and tG2 + tM of about 4 h. The estimated generation time was about 11.5 h. The results found on the hyperplastic spleen were comparable to those on the normal spleen.
mesh_terms: D000328:Adult; D000368:Aged; D002453:Cell Cycle; D002470:Cell Survival; D006410:Hematopoiesis; D006801:Humans; D006965:Hyperplasia; D008214:Lymphocytes / Q000166:cytology*; D008875:Middle Aged; D010477:Perfusion / Q000379:methods; D011868:Radioisotopes; D013154:Spleen / Q000166:cytology* / Q000473:pathology; D014316:Tritium
chemical_list: D011868:Radioisotopes; D014316:Tritium