Zn-binding protein profile of rat liver cytosol. Chromatographic and electrophoretic study.
65Zn2+ was given to rats per os and intraperitoneally for in vivo labelling of Zn2+-metalloproteins of cytosol. At the chromatographic analysis of the cytosol, two 65Zn2+-containing protein peaks were found in per os, while an additional low molecular weight third peak appeared in the intraperitoneal dosing samples. The chromatographic fractions were further separated by a non-denaturing gradient polyacrylamide electrophoresis technique. At the electrophoretic analysis of the chromatographic fractions the recovery of 65Zn2+ was the highest in samples taken from a second chromatographic peak (88%), which indicates the presence of proteins with exceptionally strong Zn2+-binding character in the 30-40 000 molecular weight fraction of the cytosol.
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D002850:Chromatography, Gel; D003600:Cytosol / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D008099:Liver / Q000032:analysis*; D008297:Male; D051381:Rats; D015032:Zinc / Q000032:analysis / Q000378:metabolism*
D002352:Carrier Proteins; C017800:zinc-binding protein; D015032:Zinc