Measurement of circulating immune complexes by radioimmunoassay of immunoglobulin in high molecular weight fractions.
A method for detecting circulating immune complexes is described based on radioimmunoassay of IgG following the rapid separation of immune complexes from monomeric IgG on short columns of Sephacryl S-300. Values obtained using sera from patients with immune complex associated diseases were distinctly higher than those obtained with sera from healthy control subjects. The same serum samples were assayed by 3 other methods for detecting immune complexes; significant correlation was obtained.
D000818:Animals; D000936:Antigen-Antibody Complex; D001172:Arthritis, Rheumatoid / Q000276:immunology; D003172:Complement C1 / Q000276:immunology; D006801:Humans; D006980:Hyperthyroidism / Q000276:immunology; D007074:Immunoglobulin G; D007136:Immunoglobulins; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008970:Molecular Weight; D011817:Rabbits; D011863:Radioimmunoassay
D000936:Antigen-Antibody Complex; D003172:Complement C1; D007074:Immunoglobulin G; D007136:Immunoglobulins