Electrophysiological and morphological changes in the guinea pig cochlea following mechanical trauma to the organ of Corti.
Small discrete lesions were produced in the organ of Corti of the guinea pig cochlea using fine probes to produce direct mechanical insult. The electrophysiological state of the cochlea was assessed using N1 electrocochleography and loss of receptor cells determined by scanning electron microscopy. Principal findings were: 1) Excellent agreement between the location of hair cell losses and the frequency of maximum sensitivity change in the N1 audiogram; 2) The spatial extent of the mechanically induced lesion appears to be more important than the total number of hair cells lost, in determining the magnitude of N1 sensitivity loss; 3) Hair cell losses extending over only 72 micrometers could be detected as significant changes in N1 sensitivity. These results further emphasize the accuracy and usefulness of the N1 electrocochleogram for assessing the functional status of the cochlea; 4) Lesions involving only outer hair cell loss also produced marked elevations of N1 threshold.
D000200:Action Potentials; D000818:Animals; D001300:Audiometry, Evoked Response / Q000379:methods; D003051:Cochlea / Q000502:physiology* / Q000648:ultrastructure; D004594:Electrophysiology; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000502:physiology / Q000648:ultrastructure; D034381:Hearing Loss / Q000209:etiology; D008855:Microscopy, Electron, Scanning; D009925:Organ of Corti / Q000502:physiology* / Q000648:ultrastructure