[Serum phospholipids determination by an automatic enzymatic method (author's transl)].
The serum phospholipids determination by an automatic enzymatic method (AEM) is suggested in this study. A comparison was made between the classical chemical method, the manual enzymatic method (MEM) and the AEM one. 100 serum samples were tested in this experiment and the correlation coefficient between these methods was found to be acceptable. The result indicates a good precision and a negligible contamination. The calibration curve is linear to 5 mmol/l. Triglyceride and cholesterol concentrations, glucose and high concentrated uric acid presence do not interfere on the value measured. However, the haemolysis and a large quantity of bilirubin in sera tend to induce a variation in the phospholipids determination.
D001322:Autoanalysis / Q000379:methods; D006801:Humans; D010544:Peroxidases; D010740:Phospholipases; D010743:Phospholipids / Q000097:blood*
D010743:Phospholipids; D010544:Peroxidases; D010740:Phospholipases
Dosage des phospholipides sériques par méthode enzymatique appliquée au flux continu.