[Importance of experimental conditions in the evaluation of the acute irritating effect of chemical compounds].
The experiment was carried out on 36 white Vienna rabbits and 27 grey rabbits of Chinchilla strain. The test substances were: sulphuric acid, acetic acid, sodium hydroxide and oil M-26. A single dermal 24 rh closed (sides) exposure, and single dermal open (sides, ears) exposure were used. The cutaneous reaction to the substances was evaluated according to Draize's et al. (1944) conventional point scale. In addition, cutaneous eruptions that have not been covered by the classification, have been included, with reactions in per cent. The intensification of the inflammatory reaction was found to depend on the way of application and duration of exposure to a given substance. The single open application resulted in cutaneous eruptions of a lesser intensity than the single closed application of the same compounds, the concentration being the same. The skin of white rabbits was found to be similarly sensitive to the irritating effect of the chemicals as the skin of grey rabbits. The histopathological examinations allowed determining the nature and localization of changes and revealed, in some cases, skin pathologies, with no clear symptoms of irritating effects of the substances revealed macroscopically.
D000085:Acetates / Q000009:adverse effects; D000208:Acute Disease; D000287:Administration, Topical; D000485:Allergens; D000818:Animals; D003877:Dermatitis, Contact / Q000209:etiology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004485:Eczema / Q000139:chemically induced; D007509:Irritants; D008899:Mineral Oil / Q000009:adverse effects; D011817:Rabbits; D012867:Skin / Q000187:drug effects; D012972:Sodium Hydroxide / Q000009:adverse effects; D013464:Sulfuric Acids / Q000009:adverse effects
D000085:Acetates; D000485:Allergens; D007509:Irritants; D013464:Sulfuric Acids; D012972:Sodium Hydroxide; D008899:Mineral Oil
Znaczenie warunków doświadczalnych w ocenie siły ostrego działania drazniacego zwiazków chemicznych.