title: Nonsense suppression in eukaryotes: the use of the Xenopus oocyte as an in vivo assay system.
abstract: Amber, ochre, and opal nonsense suppressor tRNAs isolated from yeast were injected into Xenopus laevis oocytes together with purified mRNAs (globin mRNA from rabbit, tobacco mosaic virus-RNA). Yeast opal suppressor tRNA is able to read the UGA stop codon of the rabbit beta-globin mRNA, thus producing a readthrough protein. A large readthrough product is also obtained upon coinjection of yeast amber or ochre suppressor tRNA with TMV-RNA. The amount of readthrough product is dependent on the amount of injected suppressor tRNA. The suppression of the terminator codon of TMV-RNA is not susceptible to Mg++ concentration or polyamine addition. Therefore, the Xenopus laevis oocyte provides a simple, sensitive, and well buffered in vivo screening system for all three types of eukaryotic nonsense suppressor tRNAs.
mesh_terms: D000818:Animals; D005260:Female; D005914:Globins / Q000096:biosynthesis; D007700:Kinetics; D009154:Mutation; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D012440:Saccharomyces / Q000378:metabolism; D013489:Suppression, Genetic; D014027:Tobacco Mosaic Virus / Q000378:metabolism; D014982:Xenopus laevis
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D005914:Globins; D012343:RNA, Transfer