Platelet HLA typing and cross-matching by a direct microcytotoxic assay. A new method employing carboxyfluoresceindiacetate-stained platelets.
Carboxyfluoresceindiacetate-labelled platelets can be used in direct microcytotoxic assays for the purpose of typing platelets for a number of antigenic determinants, such as HLA and beta-2 microglobulin. The assay when used in direct platelet cross-matching, where thrombocytopenic patient sera were screened, resulted in the uncovering of many extrapositive and weakly positive cross-matches not identified by the lymphocyte cross-match. The simplicity and rapidity of this new method makes it an attractive alternative for future use in research and clinical studies.
D000017:ABO Blood-Group System; D000818:Animals; D001792:Blood Platelets / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D005452:Fluoresceins; D006041:Goats; D006680:HLA Antigens; D006650:Histocompatibility Testing; D006801:Humans; D008214:Lymphocytes / Q000276:immunology
D000017:ABO Blood-Group System; D005452:Fluoresceins; D006680:HLA Antigens; C027780:carboxyfluoresceindiacetate