title: Characterization of a proteoglycan of high electrophoretic mobility.
abstract: Proteoglycans from articular cartilage of young baboons (Papio papio) were fractionated on an associative density gradient. Material from the top of the gradient was shown to contain a proteoglycan of high electrophoretic mobility on large porosity gels. Associated and/or contaminating proteins were removed by ion-exchange chromatography on DEAE-cellulose and subsequent gel filtration on Sepharose 4B in the presence of 0.1% SDS. The electrophoretically homogeneous proteoglycan (Kd 0.43 on Sepharose 4B SDS) contained 39.7% protein, was rich in aspartate, glutamate, leucine and glycine and had a GalN : GluN molar ratio of 3.87.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002358:Cartilage, Articular / Q000032:analysis*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D010215:Papio; D011509:Proteoglycans / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D011509:Proteoglycans