Pyrimidine-specific chemical reactions useful for DNA sequencing.
Potassium permanganate reacts selectively with thymidine residues in DNA (1) while hydroxylamine hydrochloride at pH 6 specifically attacks cytosine (2). We have adopted these reactions for use with the chemical sequencing method developed by Maxam and Gilbert (3).
D001483:Base Sequence; D003596:Cytosine / Q000032:analysis; D004247:DNA; D006898:Hydroxylamines; D007202:Indicators and Reagents; D008722:Methods; D011196:Potassium Permanganate; D011743:Pyrimidines / Q000032:analysis*; D013936:Thymidine / Q000032:analysis
D006898:Hydroxylamines; D007202:Indicators and Reagents; D011743:Pyrimidines; D011196:Potassium Permanganate; D003596:Cytosine; D004247:DNA; D013936:Thymidine