Uptake of nucleotides and catecholamines by chromaffin granules from pig and horse adrenal medulla.
The uptake of nucleotides and catecholamines into chromaffin granules from adrenals of pigs and horses is similar to that previously seen in bovine chromaffin granules. The rate of [3H]ATP uptake at 2 mM-ATP concentration was 0.42 +/- 0.06 and 0.15 +/- 0.02 nmol/mg protein/min for pig and horse granules, respectively. The apparent Km's were 1.37 mM for pig granules, 0.89 mM for horse granules, and 1.2 mM for ox granules. The sensitivity of the uptake for nucleotides and catecholamine to specific inhibitors was found to be similar in granules from pig and ox, indicating that the same mechanisms of uptake are involved in both species.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000313:Adrenal Medulla / Q000378:metabolism*; D000818:Animals; D001278:Atractyloside / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002417:Cattle; D002837:Chromaffin Granules / Q000187:drug effects / Q000378:metabolism*; D002838:Chromaffin System / Q000378:metabolism*; D006736:Horses; D007700:Kinetics; D009638:Norepinephrine / Q000378:metabolism*; D012110:Reserpine / Q000494:pharmacology; D013552:Swine
D001278:Atractyloside; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D012110:Reserpine; D000255:Adenosine Triphosphate; D009638:Norepinephrine