Analysis of human lymphocyte cell cycle kinetics in vitro by incorporation into DNA of bromodeoxyuridine.
Human lymphocytes of four donors were cultured for 40 to 76 hours and harvested at 4 hour intervals. They were analysed for the number of 1st, 2nd and 3rd division metaphases using the BrdU-Giemsa technique. All 44 hours cultures showed metaphases only in their 1st division; 2nd division metaphases began to appear in 48 hours cultures and 3rd division metaphases in 64 hours cultures. Another group of lymphocytes from 4 other donors were irradiated and harvested at 72 hours. These cultures showed a decrease in the percentage of 3rd division metaphases, accompanied by an increase in the percentage of 1st division metaphases, proportional to the irradiation dose.
D001973:Bromodeoxyuridine / Q000378:metabolism* / Q000494:pharmacology; D002453:Cell Cycle; D002478:Cells, Cultured; D002877:Chromosomes, Human / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D006801:Humans; D007621:Karyotyping; D007700:Kinetics; D008214:Lymphocytes / Q000502:physiology*; D008677:Metaphase; D008938:Mitosis
D004247:DNA; D001973:Bromodeoxyuridine