title: [Separation and purification of IgE and IgM by chromatographic techniques for diagnostic serology].
abstract: We describe the performance of a gel filtration technique using Sephadex G-200 and a ion exchange chromatographic separation using DEAE-cellulose. These methods are able to fractionate quickly and easily IgG and IgM as applied in diagnostic serology.
mesh_terms: D001788:Blood Grouping and Crossmatching; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D006801:Humans; D007073:Immunoglobulin E / Q000302:isolation & purification*; D007075:Immunoglobulin M / Q000302:isolation & purification*
chemical_list: D007075:Immunoglobulin M; D007073:Immunoglobulin E
vernacular_title: Separazione e purificazione di IgE e IgM mediante tecniche cromatografiche per la diagnostica sierologica.