Buffer-induced activation of calf intestinal alkaline phosphatase.
The influence of Tris, phosphate ions, imidazole, carbonate ions, bicarbonate ions and hydroxyl ions on the rate of activation of calf intestinal alkaline phosphatase after its dissolution in aqueous solution was investigated. The kinetic data are consistent with a general base-catalysed reaction in which the base reacts with an unprotonated enzyme species formed by ionization of a group with pK = 8.9.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001639:Bicarbonates; D002254:Carbonates; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004789:Enzyme Activation; D007093:Imidazoles; D007422:Intestines / Q000201:enzymology*; D007700:Kinetics; D010710:Phosphates; D013050:Spectrometry, Fluorescence; D014325:Tromethamine
D001639:Bicarbonates; D002254:Carbonates; D007093:Imidazoles; D010710:Phosphates; D014325:Tromethamine; D000469:Alkaline Phosphatase