title: Efficient incorporation of deuterated amino acids into quail egg white proteins for nuclear magnetic resonance studies.
abstract: The in vivo incorporation of deuterated amino acids into egg white proteins of Japanese quail is described. Using a synthetic diet, the level of incorporation of selectively deuterated tyrosine, tryptophan, histidine, and phenylalanine into lysozyme was greater than 80% as demonstrated by proton NMR. Load and load-chase experiments using [3H]phenylalanine or [3H]leucine monitored the fast uptake time (t 1/2 = 2 days) and confirmed the high levels of incorporation. The potential of this system for preparation of other proteins for NMR spectroscopy is discussed.
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D003370:Coturnix; D003903:Deuterium; D004527:Egg Proteins / Q000096:biosynthesis*; D004529:Egg White; D005260:Female; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009113:Muramidase / Q000096:biosynthesis; D011784:Quail
chemical_list: D000596:Amino Acids; D004527:Egg Proteins; D003903:Deuterium; D009113:Muramidase