Micro-necrotic foci in regression of a murine calvarium transplantable tumor. 2. Enzymatic modification of the transformed calvarium cell immunological characteristics.
The experiments reported in this communication examined the immunological properties of cell lines cultured under (a) normal, physiological (Cont.Cal) and (b) experimental, non-physiological (Expt.Cal) conditions described in the preceeding paper. Determination of oncogenicity, humoral and cellular immunity of the cultures at various subcultures induced in syngeneic, unconditioned CFW1 mice, showed differences between cells from the two conditions. Cells grown under the experimental conditions (Expt.Cal) became oncogenic. Oncogenicity increased with number of subcultures, and treatment with vibrio cholera neuraminidase rendered the cells non-oncogenic. It is suggested that the experimental conditions either cause malignant cell transformation, or selection of oncogenic cell variants. Since oncogenicity is reduced by treatment with neuraminidase it could be related to certain cell membrane determinants.
D000818:Animals; D000917:Antibody Formation; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology*; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000473:pathology; D003470:Culture Media; D007111:Immunity, Cellular; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology*; D009439:Neuraminidase / Q000494:pharmacology; D012886:Skull / Q000166:cytology
D003470:Culture Media; D009439:Neuraminidase