Effects of colchicine, vinblastine, griseofulvin and deuterium oxide upon phospholipid metabolism in concanavalin A-stimulated lymphocytes.
Colchicine has previously been shown to inhibit the incorporation of [3H]-inositol into phosphatidylinositol in lymphocytes stimulated with concanavalin A. In the present study other agents known to interact with tubulin or affect microtubule assembly/disassembly were examined for effects on 32PO4 incorporation into phospholipids and [3H]inositol incorporation into phosphatidylinositol. Vinblastine inhibited the enhanced incorporation caused by concanavalin A of either [3H]inositol or 32PO4 into phosphatidylinositol and phosphatidic acid. It had no effect on basal incorporation of 32PO4 and enhanced basal incorporation of [3H]inositol into nonstimulated lymphocytes. Griseofulvin at concentrations up to 10(-3) M had no effect on uptake of either label. Deuterium oxide had effects similar to vinblastine in that basal incorporation of both labels was enhanced while inhibition of the concanavalin A stimulated incorporation was observed. These results suggest an action of these various compounds other than or in addition to their known effects on cytoplasmic microtubules.
D003078:Colchicine / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology*; D003903:Deuterium / Q000494:pharmacology; D006118:Griseofulvin / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008870:Microtubules / Q000187:drug effects*; D010743:Phospholipids / Q000378:metabolism*; D014747:Vinblastine / Q000494:pharmacology
D010743:Phospholipids; D003208:Concanavalin A; D006118:Griseofulvin; D014747:Vinblastine; D003903:Deuterium; D003078:Colchicine