title: Selectivity in incorporation, utilization and retention of oleic and linoleic acids by human skin fibroblasts.
abstract: Fetal human fibroblasts were grown in culture medium containing 10% fetal bovine serum supplemented with [1(-14)C]linoleate or [1(-14)C]oleate. At all concentrations of exogenous fatty acids, the incorporation of oleate was greater than that of linoleate. With increased medium fatty acid concentrations, linoleate in triacyglycerol (TAG) could be increased from 13 to 75% of the total incorporated; at each concentration, relatively more linoleate than oleate was in TAG. When the cells were exposed to exogenous oleate/linoleate mixtures, the composition of the mixture determined the extent of incorporation of both fatty acids. When the mixture was primarily linoleate, scarce oleate was used preferentially for phospholipids (PL); no such specificity for scarce linoleate was observed. Addition of exogenous fatty acids resulted in a shift of previously incorporated 14C fatty acids from phospholipid into TAG; retention of oleate in PL was greater than that of linoleate. Incorporation of oleate into phospholipids was also higher than that of linoleate from exogenous fatty acid mixtures which were 80% saturated. It is suggested that normal human fibroblasts have adapted to the low levels of exogenous polyunsaturated fatty acids in culture media by increased use of oleate in phospholipid. Even when the cells are supplemented with linoleate, the preferential use of oleate in phospholipid groups is retained.
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D005227:Fatty Acids / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D005312:Fetal Blood; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008041:Linoleic Acids / Q000378:metabolism*; D009829:Oleic Acids / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis; D012867:Skin / Q000378:metabolism*; D014280:Triglycerides / Q000096:biosynthesis
chemical_list: D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D008041:Linoleic Acids; D009829:Oleic Acids; D010743:Phospholipids; D014280:Triglycerides