Effects of the principal hydroxy-metabolites of benzene on microtubule polymerization.
The principal hydroxy-metabolites of benzene - phenol, catechol and hydroquinone - possess characteristics and produce toxicity similar to those reported for certain inhibitors of microtubule polymerization. In this study we examined the effects of phenol, catechol and hydroquinone on purified microtubule polymerization and the decay of tubulin-colchicine binding activity. Hydroquinone, but not catechol or phenol, inhibited microtubule polymerization and accelerated the decay of tubulin-colchicine binding activity. The latter effect was shown to be dependent on the concentration of GTP. Hydroquinone did not directly complex with GTP or ATP but bound to the high molecular weight fraction of tubulin. concentration ratios of hydroquinone to tubulin resulting in altered activity were low, suggesting a specific interaction, presumably at the tubulin-GTP binding site. The acceleration of tubulin-colchicine binding activity decay was completely prevented under anaerobic conditions, indicative of an oxidative mechanism. These studies suggest that hydroquinone, which auto-oxidizes, may interfere with microtubule function, nucleotide binding or both and that this mechanism may be involved in eliciting the wide range of cytoskeletal-related abnormalities observed in cells exposed to benzene in vivo or its metabolites in vitro.
D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology; D003078:Colchicine / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006873:Hydroquinones / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D010636:Phenols / Q000494:pharmacology; D011108:Polymers / Q000378:metabolism; D051381:Rats; D014404:Tubulin / Q000378:metabolism
D001555:Benzene Derivatives; D002396:Catechols; D006873:Hydroquinones; D010636:Phenols; D011108:Polymers; D014404:Tubulin; D006160:Guanosine Triphosphate; D003078:Colchicine