Estradiol receptor activity in lyophilized calf uterus and human breast tumor tissue.
Estradiol receptors (ER) were analyzed in lyophilized human breast tumor specimen and in calf uterus tissue by a dextran-coated charcoal method. No decline of ER occurred when lyophilized calf uterus tissue was stored for six to eight months at 0-4 C and for two weeks at room temperature. During storage of lyophilized human breast tumor tissue for several months at 0-4 C and for four weeks at room temperature, no or only a minor decrease of ER binding sites was observed. Therefore, it is recommended to use lyophilized target tissues for intra- as well as interlaboratory quality control studies of steroid receptor measurements.
D000818:Animals; D001943:Breast Neoplasms / Q000032:analysis*; D002417:Cattle; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005612:Freeze Drying; D006801:Humans; D066298:In Vitro Techniques; D011960:Receptors, Estrogen / Q000032:analysis*; D013696:Temperature; D013997:Time Factors; D014599:Uterus / Q000032:analysis*
D011960:Receptors, Estrogen; D004958:Estradiol