A modified spectrophotometric test for nitroblue-tetrazolium reduction of granulocytes and monocytes and its application for testing inhibition of monocytes by serum components in tumor patients.
For evaluation of serum-factor involvement in monocyte function, we established a modified quantitative spectrophotometric-nitroblue tetrazolium (NBT) test. We investigated the stimulation of human peripheral monocytes and granulocytes by zymosan, endotoxin, and Corynebacterium parvum in vitro. In addition, polymorphonuclear neutrophil (PMN) function in a patient with non-Hodgkin lymphoma was studied during the course of polychemotherapy and intravenous application of Corynebacterium parvum (C. parvum). In non-Hodgkin lymphoma (n = 15) and bronchogenic carcinoma (n = 11) the NBT-reduction of autologous monocytes in presence of autologous serum in relation to a AB serum was decreased compared to a control group of age-matched healthy blood donors. The sera from patients with these neoplasms inhibited NBT-reduction of monocytes from healthy donors. This inhibition showed a correlation to the presence of immune complexes in patients with bronchogenic carcinoma.
D000936:Antigen-Antibody Complex; D001772:Blood Cell Count; D001984:Bronchial Neoplasms / Q000175:diagnosis*; D002283:Carcinoma, Bronchogenic / Q000175:diagnosis; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis; D008223:Lymphoma / Q000175:diagnosis*; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D009580:Nitroblue Tetrazolium; D011425:Propionibacterium acnes; D013053:Spectrophotometry; D013997:Time Factors; D015054:Zymosan / Q000494:pharmacology
D000936:Antigen-Antibody Complex; D004731:Endotoxins; D009580:Nitroblue Tetrazolium; D015054:Zymosan