[Fluorometric DNA assay at nanogram levels in biological material].
A high degree of reproducibility was obtained using a simple and rapid microfluorimetric method of DNA determination. The described method evaluates the increased fluorescence after reaction of 33258 Hoechst fluorochrome with calf thymus DNA and mouse liver chromatin preparations. The routinely used preparation reagents and the subcellular components do not interfere with the reaction.
D000818:Animals; D002417:Cattle; D002843:Chromatin / Q000032:analysis; D004247:DNA / Q000032:analysis*; D051379:Mice; D008832:Microchemistry; D013050:Spectrometry, Fluorescence
D002843:Chromatin; D004247:DNA
Dosaggio fluorimetrico del DNA a livello di nanogrammi nel materiale biologico.