Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings.
An enzyme catalysing the synthesis of sym-homospermidine from putrescine and NAD+ with concomitant liberation of NH3 was purified 100-fold from Lathyrus sativus (grass pea) seedlings by affinity chromatography on Blue Sepharose. This thiol enzyme had an apparent mol.wt. of 75000 and exhibited Michelis-Menten kinetics with Km 3.0mM for putrescine. The same enzyme activity could also be demonstrated in the crude extracts of sandal (Santalum album) leaves, but with a specific activity 15-fold greater than that in L. sativus seedlings.
D019883:Alkyl and Aryl Transferases; D002846:Chromatography, Affinity; D007887:Fabaceae / Q000201:enzymology; D007700:Kinetics; D010944:Plants / Q000201:enzymology*; D010946:Plants, Medicinal; D013095:Spermidine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D011436:Spermidine Synthase / Q000037:antagonists & inhibitors; D014166:Transferases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D014197:Trees
C007961:sym-homospermidine; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C028376:homospermidine synthetase; D011436:Spermidine Synthase; D013095:Spermidine