Fluorescence stopped-flow study of N-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide-labeled troponin C.
Static fluorescence titration and fluorescence stopped-flow kinetic experiments o N-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide-labeled troponin C showed that the molecular kinetic mechanism of the local conformational change induced by the rapid Ca2+ binding and removal reactions with the high affinity Ca2+-binding sites (sites III and IV) can be explained by the following scheme: [Formula: see text]. Numerical constants in this scheme were determined in this work.
D000818:Animals; D001665:Binding Sites; D002118:Calcium; D003374:Coumarins; D007700:Kinetics; D008301:Maleimides; D008433:Mathematics; D009124:Muscle Proteins; D009132:Muscles / Q000032:analysis; D011485:Protein Binding; D011487:Protein Conformation; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D014336:Troponin
D003374:Coumarins; D008301:Maleimides; D009124:Muscle Proteins; D014336:Troponin; C019347:N-(7-dimethylamino-4-methylcoumarinyl)maleimide; D002118:Calcium