Reversed-phase liquid chromatography of aromatic nitro compounds on a macroporous polystyrene gel.
The retention behaviour of some nitrobenzene derivatives was studied by reversed-phase chromatography on the macroporous polystyrene gel Separon SE with methanol-water, acetonitrile-water, tetrahydrofuran-water and methanol-water with adjusted pH as the eluents. The effect of the mobile phase and the presence of additional polar and non-polar substituents in the molecule of the solute on the retention were investigated. Using methanol-water (80:20) the contribution of the nitro group to the retention of the molecule is positive for substances containing both polar and non-polar functional groups. The contribution of the nitro group did not change if a mobile phase with adjusted pH was used. With acetonitrile-water and tetrahydrofuran-water the situation was more complicated.
D002853:Chromatography, Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D009578:Nitrobenzenes / Q000032:analysis*
D009578:Nitrobenzenes; D000432:Methanol