Preparative isoelectric focusing in agarose.
A method is described for preparative isoelectric focusing in agarose using low electroendosmotic agarose. Resolution comparable to that seen on analytical polyacrylamide gels is attainable as demonstrated by the isolation of bands with identical idiotypes from the serum of a patient with a monoclonal gammopathy.
D004587:Electrophoresis, Agar Gel / Q000379:methods; D006801:Humans; D006942:Hypergammaglobulinemia / Q000150:complications / Q000276:immunology; D007130:Immunoglobulin Idiotypes / Q000032:analysis; D007525:Isoelectric Focusing / Q000379:methods*; D009103:Multiple Sclerosis / Q000150:complications; D011134:Polysaccharides; D012685:Sepharose
D007130:Immunoglobulin Idiotypes; D011134:Polysaccharides; D012685:Sepharose