title: Microbial growth on 2-bromobutane.
abstract: A member of the genus Arthrobacter was isolated which grew at the expense of 2-bromobutane as sole source of carbon and energy. Evidence is presented which suggests that the initial conversion of 2-bromobutane to 2-butanol is a spontaneous chemical hydrolysis and not mediated by the organism. Further evidence from oxygen consumption experiments indicates that 2-bromobutane is oxidized through 2-butanol, methyl ethyl ketone, ethyl acetate to acetate and ethanol. Results of experiments with cells grown on pathway intermediates reveal that the enzymes necessary for the oxidation of 2-butanol, methyl ethyl ketone, ethyl acetate, ethanol and acetaldehyde are not coordinately, but individually induced by their respective substrates.
mesh_terms: D001173:Arthrobacter / Q000254:growth & development* / Q000378:metabolism; D000440:Butanols / Q000378:metabolism; D003470:Culture Media; D006842:Hydrocarbons, Brominated / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010101:Oxygen Consumption; D012988:Soil Microbiology
chemical_list: D000440:Butanols; D003470:Culture Media; D006842:Hydrocarbons, Brominated; C028005:2-bromobutane