Determination of promethazine in serum by liquid chromatography.
We describe a procedure for the liquid-chromatographic determination of promethazine in low-nanogram concentrations in serum. Triflupromazine is used as the internal standard. The method is based on a single extraction of promethazine from serum with hexane and subsequent derivatization with trichloroethyl chloroformate. Analytical recovery of promethazine is about 90%. The lower limit of detection is 1 microgram/L when a 2.0-mL aliquot of serum is assayed. Our data illustrate the practicability of the method for bioavailability studies after oral or rectal administration of promethazine hydrochloride.
D000284:Administration, Oral; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D008832:Microchemistry; D011398:Promethazine / Q000008:administration & dosage / Q000097:blood*; D012007:Rectum
D011398:Promethazine