Determination of selected pyrimidines, purines and their metabolites in serum and urine by reversed-phase ion-pair chromatography.
The qualitative and quantitative determination of selected pyrimidines, purines and azapurines and their metabolites by reversed-phase ion-pair high-performance liquid chromatography, using tetrabutylammonium hydroxide as pairing ion and isocratic chromatrographic conditions, is described. The method provides a selective and sensitive assay for these classes of compounds examined in complex biological fluids.
D000493:Allopurinol / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D011687:Purines / Q000097:blood* / Q000652:urine; D011743:Pyrimidines / Q000097:blood* / Q000652:urine; D014527:Uric Acid / Q000652:urine
D011687:Purines; D011743:Pyrimidines; D014527:Uric Acid; D000493:Allopurinol