Fluorometric detection of implantation sites in rats.
A new fluorescent technique is proposed for detecting implantation sites in the rat, based on the binding capacity of tetracycline. Both intraluminal (20 microgram/kg) and intravenous (8.3 mg/kg) administrations of tetracycline permit the macroscopic visualization (under 366 nm of ultraviolet light) of implantation sites, since they show a lower fluorescence (LFS) than the remaining endometrium (FS). Greater binding of tritiated tetracycline was observed in nonimplantation sites (176 to 56 x 10(3) dpm/mg protein) than in implantation sites (P < 0.05).
D000818:Animals; D010064:Embryo Implantation; D004717:Endometrium / Q000502:physiology*; D005260:Female; D008856:Microscopy, Fluorescence; D011247:Pregnancy; D051381:Rats; D013752:Tetracycline / Q000378:metabolism; D014599:Uterus / Q000502:physiology
D013752:Tetracycline