The uptake of IgG by human placental chorionic villi: a correlated autoradiographic and wide aperture counting study.
The uptake of 3H-IgG into human placental chorionic villi in vitro takes place for at least 1 h at 37 degrees C and at 4 degrees C. The rate of uptake is lower at the latter temperature, but still about 20 per cent of the 37 degrees C total. Measurement of cell-associated redioactivity at 4 degrees C cannot therefore be used as a measure of binding: genuine uptake, probably as a result of endocytosis, appears to occur at this temperature. Some proportion of the uptake of IgG at 37 degrees C can be inhibited by colchicine and by cytochalasin B, but some is refractory to these treatments. A coated vesicle-enriched fraction isolated from placenta previously incubated with 3H-IgG was found to be associated with radioisotope.
D001345:Autoradiography; D002478:Cells, Cultured; D002824:Chorionic Villi / Q000378:metabolism*; D004705:Endocytosis; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D013696:Temperature
D007074:Immunoglobulin G