[Method of preparing erythrocytic Fc immunoglobulin diagnostic agents].
A new method for the nonspecific sensitization of erythrocytes with IgG by means of aqueous rivanol solution has been developed. Erythrocytic diagnostic reagents are intended for detecting reagents active against the Fc fragment of IgG (staphylococcal protein A, rheumatoid factor, Clq, etc.). This method is more sensitive than Boyden's sensitization methods with the use of chronic chloride, alizarin blue indicator. The active areas of the Fab fragment of IgG are blocked on the erythrocytic surface, which constitutes an important feature of sensitization by means of rivanol.
D000818:Animals; D000918:Antibody Specificity; D004912:Erythrocytes / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D012217:Rheumatoid Factor / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013205:Staphylococcal Protein A / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology
D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D012217:Rheumatoid Factor
Sposob izgotovlenniia éritrotsitarnykh immunoglobulinovykh Fc-diagnostikumov.