title: Three day pleural inflammation: a new model to detect drug effects on macrophage accumulation.
abstract: Mononuclear cell accumulation is of major importance in maintaining chronic inflammatory conditions. In an effort to model this phenomenon, 0.3 ml of a 1% carrageenan solution was injected into the pleural cavity of rats; at various times thereafter peripheral blood and pleural exudate samples were collected. Seventy-two hours after carrageenan injection, 82.3 +/- 3.7 x 10(6) cells (N = 6; mean +/- S.E.) were present in the pleural cavity; over 80% of these cells were macrophages as determined by morphologic and histochemical criteria. Animals treated with dexamethasone had a significantly reduced number of pleural macrophages. Animals treated with the nonsteroidal anti-inflammatory agents, naproxen and indomethacin, had an elevated intrapleural macrophage content. The number of intrapleural cells was not affected by the antirheumatic agents levamisole, d- and dl-penicillamine or gold sodium thiomalate. Animals treated with tilorone, dapsone, hydroxychloroquine, phenylmethane-sulfonyl fluoride and 1,10 phenanthroline had a reduced pleural cell count.
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002351:Carrageenan / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004195:Disease Models, Animal; D007213:Indomethacin / Q000494:pharmacology; D007978:Levamisole / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D010998:Pleurisy / Q000139:chemically induced / Q000473:pathology*; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats
chemical_list: D000893:Anti-Inflammatory Agents; D011480:Protease Inhibitors; D007978:Levamisole; D003907:Dexamethasone; D002351:Carrageenan; D007213:Indomethacin