title: Determination of benzidine and 3,3'-dichlorobenzidine in wastewater by liquid chromatography with uv and electrochemical detection.
abstract: The title compounds are on the EPA priority pollutant list. Therefore, a sensitive method for their measurement is required. The liquid chromatographic method employs a LiChrosorb RP-2 column, 45% acetonitrile (pH 5) mobile phase, UV detection at 280 nm, and electrochemical detection at +0.70 volts applied potential. The three-electrode electrochemical cell described contains a tubular carbon-black/polyethylene working electrode that permits detection of sub-part-per-billion levels in a 50 microliter injection permits detection of 2 ppb benzidine and dichlorobenzidine. Recoveries from fortified wastewater of benzidine at 1 to 6 ppb and dichlorobenzidine at 3 to 12 ppb levels were 83% and 87%, respectively.
mesh_terms: D015101:3,3'-Dichlorobenzidine / Q000032:analysis*; D001560:Benzidines / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D014877:Water Pollution, Chemical / Q000032:analysis
chemical_list: D001560:Benzidines; D015101:3,3'-Dichlorobenzidine