[Quantitative determination of the o-phthaldialdehyde derivatives of noradrenaline, dopamine and normetadrenaline in urine with an amino acid analyzer (author's transl)].
Noradrenaline, dopamine and normetadrenaline in urine are separated by a cation-exchange resin (DC 6A, Durrum) and a commercially available amino acid analyzer. After separation, the o-phthalaldehyde derivatives of the compounds are formed by the usual pumping system and are quantified with a fluorimeter. Pre-packed ion-exchange columns (Bio-Rad, Munich) are used for purification and fractionation of the urine samples.
D001322:Autoanalysis; D002852:Chromatography, Ion Exchange; D004298:Dopamine / Q000652:urine*; D006801:Humans; D007202:Indicators and Reagents; D009638:Norepinephrine / Q000652:urine*; D009764:o-Phthalaldehyde
D007202:Indicators and Reagents; D009764:o-Phthalaldehyde; D004298:Dopamine; D009638:Norepinephrine
Quantitative Bestimmung der o-Phthaldialdehyd-Derivate von Noradrenalin, Dopamin und Normetanephrin aus dem Urin mit einem Aminosäureanalysator.