The source of acetyl coenzyme A for acetylcholine synthesis in the perfused rat phrenic nerve-hemidiaphragm.
Acetylcholine release by the phrenic nerve was measured in the isolated, perfused rat hemidiaphragm. In controls, the rate of release of acetylcholine increases with time; however, when (--)-hydroxycitrate, an inhibitor of ATP-citrate lyase, is added to the perfusate, the release of acetylcholine stabilizes at a level 40% below the final control value. In this preparation, the capacity of the citrate cleavage pathway to transfer acetyl coenzyme A from the nerve cell mitochondria to the cytosol increases with time; this is not the case for other transport processes.
D001275:ATP Citrate (pro-S)-Lyase / Q000037:antagonists & inhibitors; D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000109:Acetylcholine / Q000096:biosynthesis*; D000818:Animals; D002951:Citrates / Q000494:pharmacology; D003964:Diaphragm / Q000378:metabolism*; D004558:Electric Stimulation; D066298:In Vitro Techniques; D010791:Phrenic Nerve / Q000378:metabolism* / Q000502:physiology; D051381:Rats
D002951:Citrates; D000105:Acetyl Coenzyme A; C007999:hydroxycitric acid; D001275:ATP Citrate (pro-S)-Lyase; D000109:Acetylcholine