The use of Sep-Pak C18 cartridges during the isolation of gangliosides.
The use of Sep-Pak C18 reverse-phase cartridges to adsorb gangliosides from aqueous solutions was studied. When upper phases formed from chloroform-methanol tissue extracts or aqueous salt solutions containing gangliosides are rapidly passed through the cartridges, the lipids are retained and the non-lipid components can be washed through. Gangliosides and other retained lipids can subsequently be eluted with methanol or chloroform-methanol
D000818:Animals; D001923:Brain Chemistry; D002855:Chromatography, Thin Layer / Q000379:methods; D005732:Gangliosides / Q000302:isolation & purification*; D051379:Mice; D008810:Mice, Inbred C57BL; D011933:Reagent Kits, Diagnostic; D014316:Tritium
D005732:Gangliosides; D011933:Reagent Kits, Diagnostic; D014316:Tritium