A method based on the use of methanol as a stabilizing agent to prepare metal-free glyoxalase I and to reconstitute activity by addition of bivalent metal ions.
D001051:Apoenzymes / Q000378:metabolism; D001059:Apoproteins / Q000378:metabolism; D002413:Cations, Divalent; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D066298:In Vitro Techniques; D007791:Lactoylglutathione Lyase / Q000378:metabolism; D008190:Lyases / Q000378:metabolism; D008670:Metals; D000432:Methanol
D001051:Apoenzymes; D001059:Apoproteins; D002413:Cations, Divalent; D008670:Metals; D008190:Lyases; D007791:Lactoylglutathione Lyase; D000432:Methanol