The effect of lithium on the incorporation of 3H-glucosamine into glycopeptides and the transformation of 3H-glucosamine into sialic acid in rat brain.
Rats were fed a lithium-containing diet (40 mmol LiCl/kg of diet) for five weeks. The plasma-lithium concentration was 0.48 mmol Li+/l of plasma. 3H-glucosamine (400 nCi/kg body weight was injected intraperitoneally, and 24 hrs later the brain was divided anatomically into hemisphere, cerebellum, pons and thalamus. The brain parts were defatted and the sialic acid-containing glycopeptides were separated by column chromatography before determination of 3H-glucosamine, 3H-sialic acid and total sialic acid. Neither the total amount nor the specific activity of sialic acid were influenced by the lithium treatment. Also the 3H-content of the sialic acid-free glycopeptides remained unchanged.
D000818:Animals; D001711:Biotransformation; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D005944:Glucosamine / Q000378:metabolism*; D006020:Glycopeptides / Q000096:biosynthesis*; D008094:Lithium / Q000494:pharmacology*; D008297:Male; D051381:Rats; D012794:Sialic Acids / Q000096:biosynthesis*
D006020:Glycopeptides; D012794:Sialic Acids; D008094:Lithium; D005944:Glucosamine