Studies on lipase in rat brain.
Lipase activity was measured in homogenates of rat cerebral hemispheres using radioactive glycerol trioleate emulsified with Triton X-100 as substrate. The labeled oleic acid was separated from the ester with a methanol-chloroform-heptane mixture. Under these assay conditions, the activity showed pH optima at about 5.5 and 7.5. The final products of these lipase activities were suggested to be free fatty acid and glycerol.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008049:Lipase / Q000032:analysis / Q000378:metabolism*; D008297:Male; D009829:Oleic Acids / Q000378:metabolism; D051381:Rats; D014280:Triglycerides / Q000378:metabolism
D009829:Oleic Acids; D014280:Triglycerides; D008049:Lipase