Midpoint potentials of cytochromes in vesicles of anaerobically-grown Paracoccus denitrificans determined by the indirect coulometric titration method.
1. Multiplicity of redox components with spectral properties similar to b-type cytochromes was established in vesicles derived fro anaerobically-grown Paracoccus denitrificans. 2. Multiplicity of c-type cytochromes was not apparent either from low temperature spectroscopy or potentiometric titrations. 3. Cytochromes a + a3 and a component, only observable at liquid nitrogen temperature, with a spectral maximum at 582.5 nm were detected. 4. Redox cycling of electron transport components using the indirect coulometric titration method was a convenient means of pairing redox potentials and was reproducible in total absorbance changes, midpoint potentials and spectral maxima.
D000693:Anaerobiosis; D003580:Cytochromes / Q000378:metabolism*; D004563:Electrochemistry; D005615:Freezing; D007700:Kinetics; D010084:Oxidation-Reduction; D010231:Paracoccus denitrificans / Q000378:metabolism*; D011199:Potentiometry; D013053:Spectrophotometry
D003580:Cytochromes