Biliprotein assembly in the disc-shaped phycobilisomes of Rhodella violacea. Electron microscopical and biochemical analysis of B-phycoerythrin and B-phycoerythrin--C-phycocyanin aggregates.
Hexameric B-phycoerythrin (alpha beta)6 gamma is a double disc of about 10.7 x 4.3 nm; each single disc consists of a six membered periphery (alpha beta)3, the subunits of which are assumed to be associated in alternating positions with little or no staggering. A central subunit, almost certainly the gamma-subunit, penetrates both rings linking them tightly together. Hexameric C-phycocyanin (alpha beta)6 has the same construction but lacks this central subunit. In urea gel electrophoresis B-phycoerythrin I and II separate into three bands alpha, beta, and gamma in a relative molar ratio of 6:6:1. The molecular weights of the alpha-, beta-, gamma-subunits, estimated from SDS gels were 18 700, 18 700 and 29 200 and 18 300, 18 300 and 29 900 for B-phycoerythrin I and II, respectively, resulting in molecular weights of 253 600 and 249 500 for both hexameric aggregates. In density gradient centrifugation a sedimentation coefficient s20,w . 10(13) of 11.3 and a molecular weight of 244 000 were calculated. In sedimentation analyses of partially dissociated phycobilisomes a fragment consisting of two phycoerythrin hexamers with a sedimentation constant of 18 S (dodecamer) and tripartite units with two B-phycoerythrin hexamers associated with one polar C-phycocyanin hexamer with a sedimentation constant of 22 S were demonstrated. The corresponding molecular weight of the tripartite units, about 800 000, coincides well with morphological measurements on the basis of an average protein packing density and with earlier estimates on cross-linked biliprotein aggregates in gradient gel electrophoresis. The spaces of 1.2 to 3.0 nm between the hexamers give rise to a strong 6.0 nm periodicity within the tripartite units, the weak 3.0 nm periodicity originates from the double-rings of the constituent hexamers.
D002499:Centrifugation, Density Gradient; D008854:Microscopy, Electron; D008968:Molecular Conformation; D045524:Phycobilisomes; D010798:Phycocyanin / Q000378:metabolism*; D010799:Phycoerythrin / Q000378:metabolism*; D010860:Pigments, Biological / Q000378:metabolism*; D000461:Rhodophyta / Q000378:metabolism* / Q000648:ultrastructure
D045524:Phycobilisomes; D010860:Pigments, Biological; D010798:Phycocyanin; D010799:Phycoerythrin