Successful preparation of cobalt(II)-substituted hemocyanin.
The cobalt(II)-substituted hemocyanin was first prepared by dialysis of apohemocyanin against Tris-HCl buffer (pH 8.0) containing cobalt(II) ion. The amount of cobalt(II) introduced into apohemocyanin reached 47% of the total sites for copper ion in native hemocyanin, being estimated as nearly complete formation of the half-filled cobalt(II)-hemocyanin (Co(II)-Hc). The Co(II)-Hc did not bind oxygen molecule even under O2-atmosphere. The spectral data indicated that the Co(II) is in tetrahedral geometry (high-spin state).
D000818:Animals; D002942:Circular Dichroism; D003035:Cobalt; D003300:Copper; D049832:Decapodiformes; D006433:Hemocyanins; D011485:Protein Binding; D011487:Protein Conformation
D003035:Cobalt; D003300:Copper; D006433:Hemocyanins