Liquid-chromatographic quantification of plasma phenylalanine, tyrosine, and tryptophan.
Phenylalanine, tyrosine, and tryptophan are isolated and quantified by "high-pressure" liquid chromatography, with fluorescence detection. An isocratic mobile phase and reversed-phase column are used to provide rapid and reproducible measurement of these amino acids in as little as 1 to 2 microL of human plasma.
D000596:Amino Acids / Q000097:blood*; D001774:Blood Chemical Analysis / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008832:Microchemistry; D010649:Phenylalanine / Q000097:blood; D014364:Tryptophan / Q000097:blood; D014443:Tyrosine / Q000097:blood
D000596:Amino Acids; D014443:Tyrosine; D010649:Phenylalanine; D014364:Tryptophan