Metabolic inactivation of 8-methoxypsoralen (8-MOP) by rat-liver microsomal preparations.
A modified Ames assay was developed that used an arginine-requiring mutant of Chlamydomonas reinhardii as an indicator organism. By counting the numbers of colonies formed and Arg+ revertants, we studied the influence of a rat-liver S9 mix (S9 fraction plus co-factors) on the toxic and mutagenic activities of 8-MOP plus black light. Both activities were decreased after pre-incubation of 5 micrograms 8-MOP/ml with S9 mix at 37 degrees C. S9 fractions without co-factors were almost completely inactive.
D001120:Arginine / Q000235:genetics; D002696:Chlamydomonas / Q000235:genetics* / Q000528:radiation effects; D008730:Methoxsalen / Q000494:pharmacology*; D008862:Microsomes, Liver / Q000378:metabolism; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens; D014466:Ultraviolet Rays
D009153:Mutagens; D001120:Arginine; D008730:Methoxsalen