High-pressure liquid chromatographic determination of lidocaine and its active deethylated metabolites.
A simple, specific, high-pressure liquid chromatographic method for lidocaine, and its active dealkylated metabolites, monoethylglycinexylidide (I) and glycinexylidide (II), is described. Recoveries were 94% for lidocaine, 98% for I, 77% for II, and 99% for the internal standard, ethylmethylglycinexylidide (III). The sensitivity of the assay is 0.04 microgram/ml. Coefficients of variation for day-to-day replicate analyses of lidocaine and the metabolites were < 7%.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003640:Dealkylation; D006801:Humans; D008012:Lidocaine / Q000097:blood* / Q000378:metabolism; D009203:Myocardial Infarction / Q000378:metabolism
D008012:Lidocaine