Detection of lateral asymmetry in the C-bands of mouse-embryo chromosomes exposed in utero to mitomycin C.
Chromosomes from 10-day mouse embryos were C-banded and examined for aymmetric bands between sister chromatids. No occurrences of lateral asymmetry (LA) were observed in this series. However, when 10-day embryos were exposed in utero to a single maternal injection of 10 mg/kg mitomycin C 4--5 h prior to metaphase arrest with colcemid, a significant level of asymmetric C-banding was demonstrated in their chromosomes. As many as 4 LAs per cell were seen in 2 of the 50 metaphase spreads examined.
D000818:Animals; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002875:Chromosomes / Q000187:drug effects*; D004622:Embryo, Mammalian / Q000187:drug effects; D005260:Female; D008431:Maternal-Fetal Exchange; D051379:Mice; D008813:Mice, Inbred ICR; D008937:Mitomycins / Q000494:pharmacology*; D011247:Pregnancy
D008937:Mitomycins