The assembly of ribosomes in HeLa cell nucleoli.
The order in which ribosomal proteins become associated with nucleolar pre-rRNA was studied by measuring the kinetics of label accumulation in ribosomal proteins isolated from the cytoplasmic ribosomes, as well as by two-dimensional polyacrylamide gel electrophoresis of protein extracted from ribosome precursor particles. Tentative identifications of early-adding and late-adding groups of proteins have been made.
D002466:Cell Nucleolus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D012335:RNA, Ribosomal / Q000378:metabolism; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D012335:RNA, Ribosomal; D012269:Ribosomal Proteins