Biochemical evidence for two types of noradrenaline storage particles in rabbit iris.
Centrifugation techniques were used to determine the subcellular distribution of noradrenaline (NA) and dopamine beta-hydroxylase (D beta H) in the rabbit iris. By application of isopycnic and differential gradient centrifugation methods 2 types of NA vesicles could be demonstrated. Of the total particle bound NA about 70% is associated with 'light' and about 30% with 'heavy' vesicles. For both types of vesicles the distribution of D beta H reflected that of NA.
D000818:Animals; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D004285:Dogs; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism*; D005260:Female; D007498:Iris / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D009928:Organ Specificity; D009940:Organoids / Q000378:metabolism*; D011817:Rabbits; D013154:Spleen / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
D004299:Dopamine beta-Hydroxylase; D009638:Norepinephrine