Protection of mice by intraperitoneal vaccination against challenge in the foot pad with Mycobacterium marinum.
In a study of local infection of mice with Mycobacterium marinum, heat-killed M. marinum suspended either in Hanks' balanced salt solution or in Freund's incomplete adjuvant was administered i.p. to BALB/c mice. The mice were challenged in the hind foot pad 3, 7, or 14 days thereafter with 5 x 10(3) viable M. marinum. At every time interval, killed M. marinum in the saline medium conferred modest protection against the challenge infection, whereas the antigen suspended in adjuvant conferred protection only when the challenge was administered 14 days after vaccination. Freund's incomplete adjuvant appeared to enhance the infection and to minimize the immune response to the antigen.
D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage*; D005260:Female; D005502:Food; D007108:Immune Tolerance; D007156:Immunologic Memory; D007274:Injections, Intraperitoneal; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D009161:Mycobacterium / Q000276:immunology*; D009164:Mycobacterium Infections / Q000276:immunology / Q000517:prevention & control*; D014611:Vaccination
D001428:Bacterial Vaccines