[Effects of intracellular 4-aminopyridine and tetraethylammonium load on action potentials in the rabbit atrial myocardium].
Investigated are rabbit atrial trabeculae (2-4 mm length, 150-800 micrometers diameter). When using a standard microelectrode technique, the action potentials after an intracellular 4-AP and TEA load are prolonged. Only the TEA load significantly diminishes the resting potential of the beating preparations. The typical configuration of the action potential following a period of rest (rapid initial repolarization, secondary depolarization and a late retarded repolarization) is not changed qualitatively. During a repetitive stimulation after a pacing pause the action potentials' restitution is accelerated at 4-AP. After 4-AP and TEA load the secondary depolarization of the first post rest action potential is more accentuated. By reconstructing the post rest action potentials a 4-AP sensitive current is determined. At constant drive this current shows a threshold potential at -40 and a reversal potential at -10 mV. The current is inward directed between -40 and -10 mV and is outward directed at more positive potentials than -10 mV. After a pacing pause the current-voltage relation is shifted to more negative potentials. The 4-AP sensitive current is modelled by consideration of a slow activated (during the action potential) and deactivated (during the pacing pause) conductivity. Processes of accumulation are discussed.
D015761:4-Aminopyridine; D000200:Action Potentials; D000631:Aminopyridines / Q000378:metabolism*; D000818:Animals; D016275:Atrial Function; D066298:In Vitro Techniques; D008564:Membrane Potentials; D008839:Microelectrodes; D008955:Models, Cardiovascular; D009206:Myocardium / Q000378:metabolism*; D011817:Rabbits; D013757:Tetraethylammonium Compounds / Q000378:metabolism*
D000631:Aminopyridines; D013757:Tetraethylammonium Compounds; D015761:4-Aminopyridine
Wirkungen intrazellulärer 4-Aminopyridin- und Tetraäthylammonium-Beladung auf das Aktionspotential des Vorhofmyokards des Kaninchens.