Micro-necrotic foci in regression of a murine calvarium transplantable tumor. 3. Histological characteristics of the micro-necrotic foci.
The experiments reported in this communication correlate histiocytes and plasma cells infiltration into the implantation site of Expt.Cal cells, with formation of micro-necrotic foci and tumor regression. Subcutaneous implantation of any subculture from Cont.Cal cells produced lesions which disappeared 48 to 72 hours later. Cont.Cal cells from any subculture produced no tumors. After several subcultures and upon subcutaneous implantation of Expt.Cal cells lesions were formed which develop into solid tumors. The magnitude of oncogenicity of Expt.Cal cells increased with the number of subcultures. Histological examination of the site of implantation revealed actively proliferating fibrosarcoma cells. The characteristics of the cell population confirmed those of actively growing malignant cells. Multinucleated giant cells and Reed-Sternberg cells have been found in all the examined sections from implantation site of Expt.Cal cells. Treatment of oncogenic Expt.Cal cells with vibrio cholera neuraminidase (VCN) rendered the cells non-oncogenic. Inoculation of VCN-modified Expt.Cal cells induced an extensive histiocytes, lymphocytes and plasma cell infiltration leading to tumor cell destruction and formation of micronecrotic foci.
D000818:Animals; D001859:Bone Neoplasms / Q000473:pathology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D009336:Necrosis; D009368:Neoplasm Transplantation; D009439:Neuraminidase / Q000494:pharmacology; D012886:Skull / Q000166:cytology; D013154:Spleen / Q000276:immunology
D009439:Neuraminidase