title: [Diagnosis of viral A hepatitis. Evidence of anti-hepatitis A antibodies of class IgM].
abstract: A Bradley's modified procedure is described herein. It allows the diagnosis of acute viral hepatitis A by evidence of IgM anti HAV: after IgG anti-HAV have been preferentially absorbed with Staphylococcus aureus cells positive for protein A, we test for residual IgM anti-HAV in the supernatant (absorbed serum) by radioimmunoassay. We have documented the ability of this procedure to differentially detect IgM or IgG anti-HAV by analyzing 308 sera. This simple method clearly distinguishes between recent (28 cases) and past HAV infection which is very frequent in France: 80% in adults.
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral; D002648:Child; D002675:Child, Preschool; D006506:Hepatitis A / Q000175:diagnosis*; D006801:Humans; D007075:Immunoglobulin M; D007223:Infant; D008875:Middle Aged
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M
vernacular_title: A propos du diagnostic de l'hépatite virale A. Mise en évidence des anticorps anti-hépatite A de type IgM.