Isolation and partial characterization of plasma membranes from chicken liver and from Mc-29 virus induced transplantable hepatoma.
Plasma membranes have been isolated from chicken liver and from Mc-29 virus induced transplantable hepatoma. The purity of membrane preparations has been checked by electron microscopy and by determination of the activity of some enzymes: 5'-nucleotidase, Na+, K+-ATP-ase, Mg2+-ATP-ase, alkaline beta-glycerophosphatase and glucose-6-phosphatase. In hepatoma membranes the activity of 5'-nucleotidase, Na+, K+-ATP-ase and Mg2+-ATP-ase was lower, that of alkaline phosphatase higher, than in liver membrane preparation. The incorporation rate of glucosamine-14C into UDP-N-acetylglucosamine and into plasma membrane glucosamine have been studied as well. The rate of synthesis of UDP-N-acetylglucosamine was faster in liver than in tumor cells. The labeling of hepatoma plasma membranes with glucosamine-14C occurred more slowly than that of liver ones. The rate of transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to membrane-bound glucosamine is lower in hepatoma, than in liver cells.
D001355:Alpharetrovirus; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure*; D002645:Chickens; D005944:Glucosamine / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure*; D008114:Liver Neoplasms, Experimental / Q000648:ultrastructure*; D014412:Tumor Virus Infections / Q000648:ultrastructure; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism
D014537:Uridine Diphosphate N-Acetylglucosamine; D005944:Glucosamine