A spontaneous clone of Fanconi anemia fibroblasts with chromosome abnormalities and increased growth potential.
In a diploid fibroblast culture from a patient with Fanconi anemia (FA), we observed the evolution of a chromosomally abnormal clone that had an unusual proliferative advantage and increased growth potential compared with the diploid FA cells. Q-, G-, C-, and Ag-NOR banding analyses revealed that the clonal cells had a chromosomal complement 46,XY, -5, -21, +mar1, +mar2, and were trisomic for a segment of the long arm of chromosome 5 and most of the long arm of No. 21. The retention of chromosome instability in the clonal cells indicates that the alteration in growth characteristics did not result from a mutation to the wild-type allele at the FA locus.
D000741:Anemia, Aplastic / Q000235:genetics*; D002648:Child; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure*; D002904:Chromosomes, Human, 21-22 and Y / Q000648:ultrastructure; D002905:Chromosomes, Human, 4-5 / Q000648:ultrastructure; D002999:Clone Cells / Q000648:ultrastructure*; D005199:Fanconi Anemia / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008297:Male; D010641:Phenotype; D012867:Skin / Q000166:cytology