Comparison of methods of performing the radioallergosorbent test: Phadebas, Fadal-Nalebuff and Hoffman protocols.
A series of control, IgE myeloma containing and atopic sera were tested by the radioallergosorbent test using identical lots of commercially available reagents following three different RAST protocols: Phadebas, Fadal-Nalebuff and that used in our own laboratories. In addition, a new RAST tracer was evaluated in the Phadebas and Fadal-Nalebuff methods. The Phadebas technique, using the standard 0.35 PRU cut-off, was the least sensitive at 68% but its sensitivity was raised to 84% using an 0.14 PRU cut-off. The specificity was 100% at 0.35 and 95% at 0.14 PRU. Our laboratory's technique gave a sensitivity of 84% and a specificity of 100%. The Fadal-Nalebuff method had the highest sensitivity at 92% but the lowest specificity at 92%. The Fadal-Nalebuff technique leads to a significant number of false positive scores, particularly in sera with IgE > 500 U/ml or with RAST discs that have significant non-specific binding. The Fadal-Nalebuff method can be used reliably if modified to include suitable negative controls for each antigen tested.
D000485:Allergens / Q000276:immunology; D000918:Antibody Specificity; D001665:Binding Sites; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis*; D009101:Multiple Myeloma / Q000175:diagnosis*; D011852:Radioallergosorbent Test / Q000379:methods
D000485:Allergens