Regulation of the in vitro concanavalin A response of lymphocytes by cyclophosphamide-sensitive adherent cells--a possible role of Cy-sensitive regulatory cells.
On days 1--6 after cyclophosphamide (Cy) treatment, when a decrease in the number of spleen cells is observed, the stimulation of these cells by low doses of concanavalin A (ConA) is suppressed, but the response to high doses of this mitogen is enhanced. Removal of glass wool-adherent cells produces a similar bidirectional effect on the ConA dose-response; this effect is reversed upon reconstitution of these cells. Selective removal of macrophages by carrageenan does not produce this effect. Therefore, out data indicate that the primary Cy-sensitive targets are positive and negative regulatory cells, i.e. glass wool-adherent splenic cells, but are probably not macrophages.
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D003208:Concanavalin A; D003520:Cyclophosphamide