title: On the purification and mechanism of action of 5-aminoimidazole-4-carboxamide-ribonucleotide transformylase from chicken liver.
abstract: The transformylase from chicken liver catalyzing the formylation of 5-aminoimidazole-4-carboxamide ribonucleotide through the agency of 19-formyltetrahydrofolate has been purified to apparent homogeneity. Inosinicase activity copurifies. This transformylase is not further activated kinetically by the presence of the trifunctional protein in contrast to the glycinamide ribonucleotide transformylase. The enzyme exhibits a greater than 1000-fold preference for the naturally occurring 10-formyltetrahydrofolate cofactor and a sequential reaction pattern. A reinvestigation of the chemical structure of the formylated ribotide product employing 13C and 1H NMR indicated that the imidazole ring remained intact upon formylation, consistent with the originally proposed structure.
mesh_terms: D000217:Acyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002645:Chickens; D003432:Cross-Linking Reagents / Q000494:pharmacology; D019877:Hydroxymethyl and Formyl Transferases; D007096:Imidoesters / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D051027:Phosphoribosylaminoimidazolecarboxamide Formyltransferase; D011506:Proteins / Q000502:physiology; D013056:Spectrophotometry, Ultraviolet
chemical_list: D003432:Cross-Linking Reagents; D007096:Imidoesters; D046911:Macromolecular Substances; D011506:Proteins; C007471:dimethyl dithiobispropionimidate; D019877:Hydroxymethyl and Formyl Transferases; D051027:Phosphoribosylaminoimidazolecarboxamide Formyltransferase; D000217:Acyltransferases