Purification and partial characterization of a DNA polymerase alpha species from calf thymus.
We have purified a DNA polymerase alpha species from calf thymus to near homogeneity. The enzyme sediments at 5.7 S and contains two polypeptides of 123000 and 134000 daltons in about equimolar ratio. The enzyme is inhibited by aphidicolin and N-ethylmaleimide, and retains its activity in buffers containing moderate salt conditions. Activated DNA is a better substrate than poly-(dA) . (dT) 10.
D000665:Ampholyte Mixtures / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification*; D005033:Ethylmaleimide / Q000494:pharmacology; D008970:Molecular Weight; D011067:Poly dA-dT; D011487:Protein Conformation; D013379:Substrate Specificity; D013950:Thymus Gland / Q000201:enzymology*
D000665:Ampholyte Mixtures; D011067:Poly dA-dT; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase; D005033:Ethylmaleimide