Mono-oxygenase activities of coho salmon (Oncorhynchus kisutch) liver microsomes using three polycyclic aromatic hydrocarbon substrates.
1. Benzo[a]pyrene, 2,6-dimethylnaphthalene, and naphthalene were used as substrates for a coho salmon (Oncorhynchus kisutch) liver microsomal preparation. 2. The apparent Michaelis constants (Km) were as follows: benzo[a]pyrene, 2.1 microM; 2,6-dimethylnaphthalene, 15.3 microM; and naphthalene, 300 microM. 3. The results indicate that the microsomal preparation had a high affinity for 2,6-dimethylnaphthalene. Thus, 2,6-dimethylnaphthalene, an important constituent of petroleum products, is a useful substrate for research on the disposition in vivo and in vitro of xenobiotics introduced into marine environments by petroleum pollution.
D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology*; D009281:Naphthalenes / Q000378:metabolism*; D010105:Oxygenases / Q000378:metabolism*; D012474:Salmon / Q000378:metabolism*
D001580:Benzopyrenes; D009281:Naphthalenes; C028519:2,6-dimethylnaphthalene; D010105:Oxygenases