Stability of the glutamate content of synaptosomes during their preparation.
The glutamate content of synaptosomal-enriched fractions is stable during the preparation and isolation of the organelles provided that the temperature of the preparation is maintained at 0--2 degrees C and that inorganic ions are not added to the homogenizing and centrifugation media. Consequently, drug-induced in vivo changes in the glutamate content of nerve endings should be reflected by the corresponding changes in glutamate levels of synaptosomes prepared from the brain tissue.
D000818:Animals; D001923:Brain Chemistry; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D004355:Drug Stability; D005971:Glutamates / Q000032:analysis*; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D011180:Postmortem Changes; D013574:Synaptosomes / Q000648:ultrastructure*; D013696:Temperature
D005971:Glutamates