Effect of palmitic acid on regeneration of dichlorovos-inhibited plasma cholinesterase activity in vitro.
Free fatty acids (FFA) in plasma were observed to be significantly increased (p less than 0.001) in high fat diet (HFD) rats and albumin significantly decreased (p less than 0.001) in low protein diet (LPD) rats. Dichlorovos was more toxic to HFD and LPD rats, compared to high protein diet (HPD) and standard diet (SD) rats, but these differences were not statistically significant. The spontaneous regeneration of dichlorovos-inhibited plasma cholinesterase activity in vivo was faster in the HFD rats compared to the other three dietary groups. Adding palmitic acid (PA) in vitro to the plasma system, at PA/albumin molar ratios in the range 0.53:2.83, accelerated spontaneous regeneration of dichlorovos-inhibited plasma cholinesterase activity significantly during 2-24 h periods. PA acceleration of enzyme regeneration rate did not appear to be related to PA interactions with dichlorovos-albumin binding in plasma.
D000818:Animals; D002800:Cholinesterase Inhibitors; D002802:Cholinesterases / Q000097:blood*; D004006:Dichlorvos / Q000633:toxicity*; D004041:Dietary Fats / Q000008:administration & dosage; D004044:Dietary Proteins / Q000008:administration & dosage; D005230:Fatty Acids, Nonesterified / Q000097:blood; D066298:In Vitro Techniques; D008297:Male; D010169:Palmitic Acids / Q000494:pharmacology*; D051381:Rats; D012709:Serum Albumin / Q000032:analysis
D002800:Cholinesterase Inhibitors; D004041:Dietary Fats; D004044:Dietary Proteins; D005230:Fatty Acids, Nonesterified; D010169:Palmitic Acids; D012709:Serum Albumin; D004006:Dichlorvos; D002802:Cholinesterases