Evaluation of centrifugation parameters for density gradient experiments by means of a programmable pocket calculator.
A calculation program is proposed suitable for programmable pocket calculators (e.g. HP series) to estimate s20,w f omega2 dt values from density gradient centrifugation data. The program can be applied to linear or exponential density gradients prepared from sucrose or glycerol solutions spun in zonal rotors or swinging bucket rotors. A wide solute concentration range and temperature range is accounted for. Constants for empirical density calculation of glycerol and sucrose solutions concentrated in % (w/v) are estimated. Experimental verification of the program was carried out.
D002499:Centrifugation, Density Gradient / Q000379:methods*; D003201:Computers; D005990:Glycerol; D013395:Sucrose
D013395:Sucrose; D005990:Glycerol