Self-incompatibility system of Oenothera organensis for the detection of genetic effects at low radiation doses.
The self-incompatibility system of Oenothera organensis is used to analyze the frequency of S locus mutations induced by low doses (2.5-20 rad) of fast neutrons and x-rays. Th principles and methods of the screening system for detection of low dose effects have been presented. The results show that low doses induced significantly higher frequencies of seeds and seedlings than those produced spontaneously. The analyses of part of the progenies derived from the control and treated series suggested that they were due to revertible mutations. A modified in vitro method of culturing pollinated cut styles has been developed for rapid screening of compatible pollen tubes.
D046508:Culture Techniques; D005214:Fast Neutrons; D005298:Fertility; D009154:Mutation; D010944:Plants / Q000235:genetics* / Q000528:radiation effects; D011058:Pollen; D011829:Radiation Dosage