[Simultaneous registration of pyridine nucleotides and flavoproteins in contracting myocardium by the luminescence method].
A luminescent divice has been elaborated for a simultaneous registration of the dynamics of NADH oxidation and of flavoprotein reduction in the beating heart muscle. The device is based on a microfluorimeter with alternating luminescence excitation in two-wave lenths--365 and 410 nm. The technique proposed makes it possible to minimize the reciprocal contribution of either carrier to the resulting measurement of their fluorescence intensity.
D000818:Animals; D005420:Flavoproteins / Q000032:analysis*; D005453:Fluorescence; D008163:Luminescent Measurements; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism*; D009243:NAD / Q000032:analysis*; D010084:Oxidation-Reduction; D011817:Rabbits; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
D005420:Flavoproteins; D009243:NAD
Odnovremennaia registratsiia pridinnukleotidov i flavoproteinov v sokrashcheĭsia serdechnoĭ myshtse liuminestsentnym metodom.