Histochemical demonstration of thiols and disulfides by the fluorescent labeling agent, monobromobimane: an application to the hypothalamo-neurohypophysial system.
The use of the new fluorescent labeling agent monobromobimane for the histological visualization of thiols, and, after reduction, of disulfides, is described. Fluorescent labeling of the disulfide-rich neurosecretory proteins in the hypothalamo-neurohypophysial system of rat and frog is shown. The reaction of the non-fluorescent reagent with thiols results in highly fluorescent materials which resist fading. The labeling procedure is very simple, rapid, and is carried out in one step. It is non-destructive and can allow for biochemical analyses of the labeled tissue.
D000818:Animals; D001001:Anura; D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D004220:Disulfides / Q000032:analysis*; D004229:Dithiothreitol; D005033:Ethylmaleimide; D005456:Fluorescent Dyes; D008856:Microscopy, Fluorescence; D010902:Pituitary Gland / Q000033:anatomy & histology*; D051381:Rats; D013438:Sulfhydryl Compounds / Q000032:analysis*
D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D004220:Disulfides; D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; D005033:Ethylmaleimide; D004229:Dithiothreitol; C028381:monobromobimane