[Rapid spectrophotometric method for determining ornithine decarboxylase using 2,4-dinitrofluorobenzene].
A sensitive spectrophotometric micromethod was developed for estimation of ornithine decarboxylase (ODC) activity in animal tissues. Distinct increase in the enzymatic activity was found in regenerating and malignant liver tissues as well as in hepatomas G-27 and G-46 as compared with the corresponding tissues of control animals. The linear relationship between the optic density and content of dinitrophenyl putrescine was maintained over the range of 0 to 150 nmol per a sample. The ODC pH optimum was observed within the zone pH 6.4-6.8; it did not depend on the nature of the buffer, used for tissue homogenization. The recovery of putrescine added into the sample was equal to 102.97 +/- 2.22%. The method had an error less than 3%, its sensitivity was 0.1 nmol/sample and the reproducibility was about 100%.
D000818:Animals; D002262:Carboxy-Lyases / Q000032:analysis*; D004052:Diethylnitrosamine; D004139:Dinitrofluorobenzene; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology*; D008115:Liver Regeneration; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D009578:Nitrobenzenes; D009602:Nitrosamines; D009955:Ornithine Decarboxylase / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods
D009578:Nitrobenzenes; D009602:Nitrosamines; D004052:Diethylnitrosamine; D004139:Dinitrofluorobenzene; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase
Bystryi spectrophotometricheskii metod opredeleniia ornitindekarboksilazy s pomoshch'iu 2,4-dinitroftorbenzola.