title: Intervening sequences in a Dictyostelium gene that encodes a low abundance class mRNA.
abstract: Using S1 nuclease protection experiments and DNA sequencing, we have identified two intervening sequences (introns) within a Dictyostelium gene that codes for a low abundance class mRNA. The two introns are located within the protein coding region of this gene. Both are small (approximately 100 bp) and extremely (approximately 95%) A + T rich. The splice junction sequences are similar to the splice sites in other eukaryotes. Finally, we have shown that these introns are transcribed as part of a higher molecular weight nuclear precursor.
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D004023:Dictyostelium / Q000235:genetics*; D005796:Genes; D009694:Nucleic Acid Precursors / Q000235:genetics; D010957:Plasmids; D012331:RNA, Fungal / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D009694:Nucleic Acid Precursors; D012331:RNA, Fungal; D012333:RNA, Messenger