Role of complement in porphyrin-induced photosensitivity.
Addition of porphyrins to sera of guinea pigs in vitro, followed by irradiation with 405 nm light, resulted in dose-dependent inhibitions of hemolytic activity of complement (CH50, C3, and C5). With guinea pig as an animal model, we also found that systemically administered porphyrins, followed by irradiation with 405 nm light, resulted in dose-dependent inhibition of CH50 in vivo. The erythrocytes from porphyrin-treated guinea pigs showed an increased susceptibility to hemolysis induced by 405 nm irradiation in vitro. Clinical changes in these animals were limited to light-exposed areas and consisted of erythema, crusting, and delayed growth of hair. Histologically, dermal edema, dilation of blood vessels, and infiltration of mononuclear and polymorphonuclear cells were observed. Guinea pigs irradiated with ultraviolet-B developed erythema, but had no alteration of their complement profiles. It is suggested that complement products may play a specific role in the pathogenesis of the cutaneous lesions of some porphyrias.
D000818:Animals; D003176:Complement C3 / Q000378:metabolism; D003182:Complement C5 / Q000378:metabolism; D003165:Complement System Proteins / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D006168:Guinea Pigs; D006461:Hemolysis / Q000187:drug effects; D066298:In Vitro Techniques; D008027:Light / Q000009:adverse effects; D010787:Photosensitivity Disorders / Q000097:blood / Q000139:chemically induced* / Q000473:pathology; D011166:Porphyrins / Q000494:pharmacology; D012867:Skin / Q000473:pathology
D003176:Complement C3; D003182:Complement C5; D011166:Porphyrins; D003165:Complement System Proteins