Effect of ethanol on microsomal metabolism of dimethylnitrosamine.
Rat liver microsomes were prepared from male and female controls and from animals pretreated for 3 weeks with ethanol, and incubated with dimethylnitrosamine (DMN) and an NADPH-regenerating system. The formation of formaldehyde and nitrite as well as the alkylation of microsomal protein were found to be greatly enhanced, especially in the low DMN concentration range, as a result of long-term ethanol induction. In contrast, ethanol or tetrahydrofuran, when incubated simultaneously with DMN, inhibited microsomal metabolism of the carcinogen.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004052:Diethylnitrosamine / Q000378:metabolism; D004128:Dimethylnitrosamine / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats
D004052:Diethylnitrosamine; D000431:Ethanol; D003577:Cytochrome P-450 Enzyme System; D004128:Dimethylnitrosamine