Collagen localization in normal and fibrotic human skeletal muscle.
The distribution of types I to IV collagen, types I and III p-N collagen, and fibronectin in human skeletal muscle was studied by immunofluorescence using purified antibodies to those proteins. In normal muscle, types I and III collagen, types I and III p-N collagen, and fibronectin were localized in the endomysium and perimysium. Type IV collagen was restricted to basement membrane. Type II collagen was not present. In Duchenne's musclar dystrophy and dermatomyositis/polymyositis (DM/PM), the prominently increased endomysial and perimysial fibrosis consisted of types I and III collagen, types I and III p-N collagen, and fibronectin. In DM/PM, thickening of the walls of perimysial venular and arteriolar vessels was associated with accumulation of types I and III collagen, types I and III p-N collagen, and fibronectin, as well as type IV collagen. There was no disease-specific accumulation of collagen, p-N collagen, or fibronectin.
D000293:Adolescent; D000906:Antibodies / Q000276:immunology; D001808:Blood Vessels / Q000032:analysis; D002648:Child; D003094:Collagen / Q000032:analysis* / Q000145:classification / Q000276:immunology; D003238:Connective Tissue / Q000032:analysis; D003882:Dermatomyositis / Q000378:metabolism* / Q000473:pathology; D005347:Fibroblasts; D006801:Humans; D009132:Muscles / Q000032:analysis* / Q000033:anatomy & histology; D009136:Muscular Dystrophies / Q000378:metabolism* / Q000473:pathology; D009220:Myositis / Q000378:metabolism* / Q000473:pathology
D000906:Antibodies; D003094:Collagen