title: Identification of the sialic acids from the egg jelly coat of the sea urchin Pseudocentrotus depressus (Okayama).
abstract: The jelly coat substance of the sea urchin eggs of Pseudocentrotus depressus (Okayama) was found to contain 0.35% sialic acids. After isolation of the sialic acids from the purified sulfated sialoglycoprotein containing 11% of sialic acids, analysis by colorimetry, thin-layer chromatography and gas-liquid chromatography/mass spectrometry demonstrated the nature of the sialic acids to be N-glycolylneuraminic acid and 9-O-acetyl-N-glycoloylneuraminic acid. The molar ratio between both sialic acids was found to be 1 : 1. Since it is known that O-acyl groups are partially released during the hydrolysis and isolation procedure, it can be assumed that in the native glycoprotein the percentage of O-acetylation is much higher than 50%. The sugar and sulfate composition of the glycoprotein is also described.
mesh_terms: D000818:Animals; D002855:Chromatography, Thin Layer; D003124:Colorimetry; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D010063:Ovum / Q000032:analysis*; D012617:Sea Urchins; D012794:Sialic Acids / Q000032:analysis*; D012795:Sialoglycoproteins / Q000302:isolation & purification
chemical_list: D012794:Sialic Acids; D012795:Sialoglycoproteins