The effects of a soluble factor released by sensitized mononuclear cells incubated with S. haematobium ova on eosinophil migration.
Incubation of sensitized mononuclear cells from patients with schistosomiasis with specific antigen containing a suspension of viable Schistosoma haematobium ova resulted in the release of a soluble factor which was chemotactic for eosinophils. Production of this substance was detectable at 24 h and demonstrated peak chemotactic activity after 2 days in culture. The chemotactic potential was dose-dependent and attracted eosinophils obtained from patients with schistosomiasis or allergic diathesis. Human neutrophil motility was unaffected by this chemoattractant. Preliminary studies demonstrated that the chemotactic factor is a heat-stable substance with peak activity associated with a molecular weight of approximately 42,000. These findings may reflect an in vitro correlate of cell-mediated immunity and may indicate a role played by the lymphocyte in the control of eosinophil function in human biology.
D000941:Antigens / Q000276:immunology; D002465:Cell Movement; D002630:Chemotactic Factors / Q000096:biosynthesis*; D002631:Chemotactic Factors, Eosinophil / Q000096:biosynthesis*; D002634:Chemotaxis, Leukocyte; D002850:Chromatography, Gel; D004804:Eosinophils / Q000502:physiology; D006358:Hot Temperature; D006801:Humans; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008970:Molecular Weight; D009504:Neutrophils / Q000502:physiology; D012548:Schistosoma haematobium / Q000276:immunology; D012552:Schistosomiasis / Q000276:immunology*; D013997:Time Factors
D000941:Antigens; D002630:Chemotactic Factors; D002631:Chemotactic Factors, Eosinophil