[Measurement of the hemoglobin content of fresh blood by atomic-absorption-spectrophotometric iron determination (author's transl)].
Description of an atomic-absorption-spectrophotometric iron determination in whole blood for the measurement of hemoglobin content. Its use is recommended for Hb determination in decayed blood because in such cases the photometric Cyan-Met-Hb method is not always reliable. To 5 ml aqueous Triton-X-100 solution (0.5%) 0.1 ml blood are added. Measuring is done after hemolysis has occurred. The calibration of the atomic-absorption spectrophotometer is performed with a standard of 10 microgram Fe/ml H2O. Samples with a Fe-content from 1 microgram to 20 microgram/ml H2O (1.6 g--32 g Hb/100 ml) are in the linear range of the apparatus. There is a linear correlation between the results of this and the photometric method.
D006452:Hemoglobinometry / Q000379:methods*; D006801:Humans; D007501:Iron / Q000097:blood*; D008706:Methemoglobin / Q000032:analysis; D013054:Spectrophotometry, Atomic / Q000379:methods*
D008706:Methemoglobin; D007501:Iron
Messung des Hämoglobingehalts von frischem Blut durch atomabsorptionsspektralphotometrische Eisenbestimmung.