Chlorinated phenoxyacetic acids and chlorophenols in the modified Allium test.
The chlorinated phenoxyacetic acids 2-methyl-4-chlorophenoxyacetic acid (MCPA), 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) and 2,4,5-T butoxyethyl ester and the chlorophenols 2,4-dichlorophenol and 2,4,5-trichlorophenol were tested for genotoxicity in the modified Allium test, which is based on exposure to the test chemicals of growing onions. The mean length of growing roots were measured and chromosome damage was recorded. Of the substances tested, MCPA was the most toxic and the chlorophenoxyacetic acids were more toxic than the chlorophenols. The lower threshold values for growth retardation were below 0.1 ppm for the acids, approx. at 0.1 ppm for the ester and less than 5 ppm for the phenols. Though a monocotyledon, Allium cepa was sensitive enough to respond to even low concentrations of these dicotyledon-selecting pesticides.
D015085:2,4,5-Trichlorophenoxyacetic Acid / Q000494:pharmacology; D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D008456:2-Methyl-4-chlorophenoxyacetic Acid / Q000494:pharmacology; D002733:Chlorophenols / Q000494:pharmacology*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006016:Glycolates / Q000494:pharmacology*; D010945:Plants, Edible / Q000187:drug effects / Q000254:growth & development
D002733:Chlorophenols; D006016:Glycolates; D015084:2,4-Dichlorophenoxyacetic Acid; C009534:2,4,5-trichlorophenol; D015085:2,4,5-Trichlorophenoxyacetic Acid; D008456:2-Methyl-4-chlorophenoxyacetic Acid; C004762:2,4-dichlorophenol