Conversion of 3T3-L1 fibroblasts to fat cells by an inhibitor of methylation: effect of 3-deazaadenosine.
3-Deazaadenosine, an inhibitor of methylation, increased the frequency of conversion of 3T3-L1 fibroblasts to fat cells in a dose-dependent manner. Once converted, the 3T3-L1 fat cells retained their adipose morphology and accumulated triglycerides even when 3-deazaadenosine was removed from the culture medium. 3-Deazaadenosine may perturb cellular methylation and thereby lead to an increase in the frequency of differentiation of 3T3-L1 fibroblasts to fat cells.
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002331:Carnitine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology; D008745:Methylation; D051379:Mice; D012263:Ribonucleosides / Q000494:pharmacology*; D014372:Tubercidin / Q000494:pharmacology*
D012263:Ribonucleosides; C018258:3-deazaadenosine; D014372:Tubercidin; D002331:Carnitine