Benzo(a)pyrene metabolism and metabolites binding to DNA in the presence of BHA.
The effect of antioxidant butylated hydroxyanisole (BHA) on benzo(a)pyrene (BP) activation by human skin in vitro was investigated. BP metabolism and metabolites binding to DNA was determined after 24 and 48 hours of skin cultivation with BP or BP and BHA. The results showed that BHA increased of total BP metabolism and amount of water soluble metabolites. BHA reduced to about 50% amount of ethyl-acetate soluble metabolites and metabolites binding to DNA. These data suggest that BHA maybe stimulates enzyme activities in human skin leading to the increase of total BP metabolism and amount of water soluble metabolites what gives a decrease in binding to DNA.
D000293:Adolescent; D000368:Aged; D000840:Anisoles / Q000494:pharmacology*; D001580:Benzopyrenes / Q000378:metabolism*; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D046508:Culture Techniques; D004247:DNA / Q000378:metabolism; D005260:Female; D006801:Humans; D012867:Skin / Q000378:metabolism*
D000840:Anisoles; D001580:Benzopyrenes; D002083:Butylated Hydroxyanisole; D004247:DNA