Identification of canine urinary metabolites of the antiprotozoal agent 3a,4,5,6,7,7a-hexahydro-3-(1-methyl-5-nitro-1H-imidazol-2-yl)-1,2-benzisoxazole .
Metabolite fractions from the urine of a dog dosed with 3a,4,5,6,7a-hexahydro-3-(1-methyl-5-nitro-1H-imidazol-2-yl)-1,2-benzisoxazole (MK-0436) were obtained by the use of high-performance liquid chromatography. These fractions were of suitable purity for structural elucidation. Data obtained by mass spectrometry and NMR spectroscopy allowed the identification of seven major metabolites of this drug. Biotransformation in each case involved hydroxylation (mono or di) of the hexahydrobenzisoxazole ring.
D000818:Animals; D000981:Antiprotozoal Agents / Q000378:metabolism / Q000652:urine*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry; D007555:Isoxazoles / Q000378:metabolism / Q000652:urine*; D009682:Magnetic Resonance Spectroscopy; D009593:Nitroimidazoles / Q000378:metabolism / Q000652:urine*; D010080:Oxazoles / Q000652:urine*
D000981:Antiprotozoal Agents; D007555:Isoxazoles; D009593:Nitroimidazoles; D010080:Oxazoles; C022191:MK 436