Solid phase radioimmunoassay for detection of circulating food protein antigens in human serum.
A two site solid phase radioimmunoassay for detection of common food antigens is described. Bovine serum albumin, beta-lactoglobulin and ovalbumin can be detected in normal human serum at levels ranging from 0.1 to > 1000 ng/ml; sensitivity is not impaired by the presence of low levels of antibodies. Thirty min to 3 h after oral intake of milk, beta-lactoglobulin could be detected in the sera of 3 normal individuals, at a concentration of 0.1-3 ng/ml. This assay should prove useful in assessing the importance of macromolecular absorption in food allergy and in other gastrointestinal diseases.
D000328:Adult; D000485:Allergens / Q000276:immunology*; D000818:Animals; D002417:Cattle; D005502:Food; D005512:Food Hypersensitivity / Q000097:blood* / Q000378:metabolism; D006801:Humans; D007408:Intestinal Absorption; D007782:Lactoglobulins / Q000276:immunology; D008892:Milk / Q000009:adverse effects; D010047:Ovalbumin / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000276:immunology
D000485:Allergens; D007782:Lactoglobulins; D012710:Serum Albumin, Bovine; D010047:Ovalbumin