Induction by phorbol esters of macrophage differentiation in human leukaemia cell lines does not require cell division.
Phorbol esters can induce differentiation into macrophage-like cells in the two human myeloid leukaemia cell lines HL-60 and KG-1. We investigated the requirement for DNA synthesis in this differentiation process. We found that phorbol ester-induced differentiation of blast cells into cells with the morphologic, histochemical, and phagocytic characteristics of macrophages did not require 3H-thymidine incorporation. Our results indicate that phorbol ester induced macrophage differentiation does not require DNA synthesis.
D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D008264:Macrophages / Q000187:drug effects*; D010587:Phagocytosis; D010703:Phorbol Esters / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*
D010703:Phorbol Esters; D010704:Phorbols; D004247:DNA