[Experimental analysis of embichin interaction with human serum albumin].
Study of embichin binding to human serum albumin in vitro by gel chromatography and fluorescence has shown that embichin is bound to human serum albumin with an association constant of 3.3 x 10(3) M-1. Interaction of embichin and albumin, that is reversible in the initial stages, transforms to irreversible on long-term contact of the protein with the drug. Experiments with cell culture indicate that the interaction with albumin potentiates the cytotoxic action of embichin.
D000478:Alkylation; D002850:Chromatography, Gel / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005021:Ethylamines / Q000097:blood*; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D008466:Mechlorethamine; D011485:Protein Binding / Q000187:drug effects; D012709:Serum Albumin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013997:Time Factors
D005021:Ethylamines; D012709:Serum Albumin; D008466:Mechlorethamine
Eksperimental'nyĭ analiz vazimodeĭstviia émbikhina s syvorotochnym al'buminom cheloveka.