Methylamine stimulates the action of ricin toxin but inhibits that of diphtheria toxin.
The action of ricin toxin was stimulated by addition of methylamine or some other amines, as shown by measuring the inhibition of protein synthesis of cultured cells by the toxin. Under the same conditions, however, the action of diphtheria toxin was completely inhibited by the amines. In a cell-free protein-synthesizing system, methylamine had no effect on the action of the A chain of ricin toxin and fragment A of diphtheria toxin. Studies on the interactions of 125I-labeled toxins with cells revealed that methylamine did not alter toxin-receptor bindings, but affected the entry of the toxins into the cells. Studies were also made on the effects of methylamine on the actions of two hybrid toxins, formed from a subunit of Wistaria floribunda lectin and fragment A of diphtheria toxin and the A chain of ricin toxin, respectively. Results suggested that the processes of entry of ricin toxin and diphtheria toxin, or at least parts of these processes, are different.
D002474:Cell-Free System; D004167:Diphtheria Toxin / Q000494:pharmacology*; D007700:Kinetics; D037102:Lectins; D008744:Methylamines / Q000494:pharmacology*; D037121:Plant Lectins; D010944:Plants / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D055503:Protein Multimerization; D012276:Ricin / Q000494:pharmacology*; D014908:Triticum / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D004167:Diphtheria Toxin; D037102:Lectins; D008744:Methylamines; D037121:Plant Lectins; D012276:Ricin