Identification and distribution of m- and p-hydroxyphenylacetic acids in the brain of the rat.
The m and p isomers of hydroxyphenylacetic acid have been identified and quantitated in whole rat brain and in several regions using a capillary column high resolution gas chromatography - mass spectrometry procedure. Their concentrations were: for m-hydroxyphenylacetic acid (mean +/- S.E., number of determinations in parentheses)-whole brain, 2.3 +/- 0.3 ng/g (7); hypothalamus, 1.2 +/- 0.3 ng/g (5); caudate nucleus, 5.5 +/- 0.6 ng/g (5); brain stem, 1.8 +/- 0.1 ng/g (5); cerebellum, 1.2 +/- 0.1 ng/g (5) and the "rest," 1.7 +/- 0.1 ng/g (5); and for p-hydroxyphenylacetic acid-whole brain, 10.6 +/- 0.7 ng/g (7); hypothalamus, 4.5 +/- 0.1 ng/g (4); caudate nucleus, 28.3 +/- 1.6 ng/g (5); brain stem, 8.6 +/- 0.6 ng/g (5); cerebellum, 8.1 +/- 0.4 ng/g (5), and the "rest," 5.3 +/- 0.5 ng/g (5). This heterogeneous distribution parallels closely that exhibited by their respective precursor amines, m- and p-tyramine.
D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D001933:Brain Stem / Q000032:analysis; D002421:Caudate Nucleus / Q000032:analysis; D002531:Cerebellum / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D007031:Hypothalamus / Q000032:analysis; D008297:Male; D010648:Phenylacetates / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats; D014439:Tyramine / Q000032:analysis / Q000378:metabolism
D010648:Phenylacetates; C008070:4-hydroxyphenylacetic acid; D014439:Tyramine