Determination of enzyme kinetic parameters by continuous addition of substrate to a single reaction mixture and analysis by a tangent-slope procedure. II. Application of the method to soluble and immobilized enzymes.
D000818:Animals; D001322:Autoanalysis / Q000295:instrumentation; D004798:Enzymes / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D011817:Rabbits; D013552:Swine
D004798:Enzymes; D004800:Enzymes, Immobilized; D007770:L-Lactate Dehydrogenase