R-loop hybridization of collagen DNA: separation in Cs2SO4 gradients.
High molecular weight poly(A)RNA (26-35 S) from chicken embryo trunks which is enriched for collagen mRNA was iodinated and hybridized to DNA under conditions of R-loop formation. The R-loops were separated in Cs2SO4 gradients from the bulk of DNA yielding double stranded DNA enriched for collagen genes.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D003094:Collagen / Q000235:genetics; D004247:DNA / Q000302:isolation & purification*; D066298:In Vitro Techniques; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; D003094:Collagen; D004247:DNA