A new technique for the cytochemical examination of human hemopoietic cells grown in agar gel.
In this paper, a new technique for the cytochemical examination of human hemopoietic cells grown in agar gel is described. All colonies in the agar gel in a plastic dish can be observed as a whole plate preparation after being stained cytochemically. Granulocytes and monocytes/macrophages in a cluster or a colony were easily distinguished from each other by esterase activity in response to naphthol AS-D chloroacetate of alpha-naphthyl acetate. To illustrate this technique's possibilities a time course study on the formation of granulocyte and monocyte/macrophage clusters or colonies from normal marrow cells was performed.
D000362:Agar; D002478:Cells, Cultured; D004950:Esterases / Q000378:metabolism; D005782:Gels; D006098:Granulocytes; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006651:Histocytochemistry; D006801:Humans; D008264:Macrophages; D009000:Monocytes; D009283:Naphthol AS D Esterase / Q000378:metabolism; D013997:Time Factors
D005782:Gels; D000362:Agar; D004950:Esterases; D009283:Naphthol AS D Esterase