title: Purification and structural characterization of rat liver threonyl transfer ribonucleic acid synthetase.
abstract: Threonyl-tRNA synthetase was purified approximately 500-fold from a high-speed supernatant fraction of rat liver. The purified enzyme was estimated to be > 95% pure from acrylamide gel electrophoresis under denaturing and nondenaturing conditions. Based on a native molecular weight from sedimentation equilibrium of 154000 and a subunit molecular weight of 85000 obtained bo sodium dodecyl sulfate gel electrophoresis, the protein appears to be an alpha 2 dimer. The alpha 2 structure was also supported by cross-linking studies of the native enzyme. The purified protein has an S20,w of 7.2 and an isoelectric point of 6.4. Amino acid analyses revealed no unusual features, but attempts at automated sequence analyses suggested that both amino termini are blocked. Preliminary carbohydrate analyses suggested that the enzyme is a glycoprotein. Antibodies were raised against the purified protein which could inactivate and precipitate threonyl-tRNA synthetase.
mesh_terms: D000327:Adsorption; D000537:Aluminum Oxide; D000596:Amino Acids / Q000032:analysis; D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D000818:Animals; D002130:Calcium Phosphates; D002241:Carbohydrates / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D011817:Rabbits; D051381:Rats; D013053:Spectrophotometry; D013914:Threonine-tRNA Ligase / Q000276:immunology / Q000302:isolation & purification*; D014461:Ultracentrifugation
chemical_list: D000596:Amino Acids; D002130:Calcium Phosphates; D002241:Carbohydrates; D000604:Amino Acyl-tRNA Synthetases; D013914:Threonine-tRNA Ligase; D000537:Aluminum Oxide