Diffusional limitations in liquid-phase reactions catalyzed by enzymes immobilized on porous supports: sucrose inversion by beta-fructooxidase immobilized on IRA-93 resin.
An experimental procedure has been proposed to study liquid-phase reactions catalyzed by enzymes immobilized on porous supports. The kinetic behavior can be assessed by experimental runs which allow a separate evaluation of the internal diffusion, the surface kinetics, and the fluid-particle mass transfer. The method has been applied in the study of sucrose inversion by beta-fructooxidase immobilized on IRA-93 resin.
D004800:Enzymes, Immobilized / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D012116:Resins, Plant; D013393:Sucrase / Q000378:metabolism*; D013395:Sucrose; D009626:Terminology as Topic; D043324:beta-Fructofuranosidase
D004800:Enzymes, Immobilized; D012116:Resins, Plant; D005632:Fructose; D013395:Sucrose; C028629:IRA-93 resin; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase; D013393:Sucrase