title: Enzyme release from isolated erythrocytes and lymphocytes in Duchenne muscular dystrophy.
abstract: The release of lactate dehydrogenase (LDH) was studied in erythrocytes and lymphocytes from patients with Duchenne muscular dystrophy. Erythrocytes were incubated in Krebs-Ringer with or without 30 mM adenosine and in autologous plasma. There was little release of enzyme in Krebs-Ringer solutions up to 24 h or in autologous plasma up to 48 h, with no statistical difference between Duchenne patients and controls. Addition of 30 mM adenosine reduced the release of LDH in Duchenne erythrocytes at 48 h from 32.4 +/- 5.66% (SE) to 6.2 +/- 1.76% (P < 0.05) and in controls from 38.1 +/- 5.93% to 23.5 +/- 4.63% (n.s.). Lymphocytes incubated in Krebs-Ringer containing 33 mM glucose released LDH faster than Duchenne patients and controls. The adenosine effect could be due to abnormal adenosine uptake (because of a generalized membrane defect) or indicate intrinsic differences in the adenine nucleotide metabolism.
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D009136:Muscular Dystrophies / Q000201:enzymology*
chemical_list: D007770:L-Lactate Dehydrogenase