Purification of smooth-muscle desmin and a protein-chemical comparison of desmins from chicken gizzard and hog stomach.
A fast and convenient procedure for the purification of polymerization-competent smooth-muscle desmin is described. Desmin from chicken gizzard and hog stomach were compared by fingerprint techniques. The two proteins show extensive amino acid sequence homology, although some clear differences in the peptide patterns are indicated. Comparative amino acid sequence analysis of some of the peptides obtained in pure form directly proves this conclusion.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002645:Chickens; D003893:Desmin; D005895:Gizzard, Avian / Q000032:analysis*; D008854:Microscopy, Electron; D008970:Molecular Weight; D009124:Muscle Proteins / Q000302:isolation & purification*; D009130:Muscle, Smooth / Q000032:analysis*; D009928:Organ Specificity; D013270:Stomach / Q000032:analysis*; D013552:Swine
D000596:Amino Acids; D003893:Desmin; D009124:Muscle Proteins