The development of a rapid and sensitive method, based on hemagglutination inhibition, for the measurement of cow milk in goat milk.
The immunisation of goats with bovine IgG1 provides antisera capable of measuring the protein in cow milk. The serum specificity is absolute and there is no cross reaction with goat milk. The antiserum agglutinates bovine IgG1 sensitized hen red cells except when its activity has been neutralized by preincubation with goat milk containing milk added fraudulently. This hemagglutination inhibition reaction, carried out on microtiter plates allows the detection of one part of cow milk in 1000 parts goat milk within 30 minutes.
D000818:Animals; D002417:Cattle; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D006385:Hemagglutination Inhibition Tests; D008892:Milk / Q000032:analysis*