Unscheduled DNA synthesis in cultured ataxia telangiectasia fibroblast-like cells.
The capacity of cultured fibroblast-like cells from 5 ataxia telangiectasia (AT) patients to repair gamma-ray-induced damage formed in DNA under aerobic conditions was measured by unscheduled DNA synthesis (UDS). The AT cultures exhibited generally reduced UDS compared to the normal cultures for gamma-ray doses of 4--12 kR. Reduced UDS in the AT cultures was observed for repair-incubation periods at which UDS was not saturated (0.5 and 1 h), and for periods characterized by maximal amounts of UDS in both the normal and AT cultures (2 and 4 h). The results suggest a class of lesions repairable in the normal cells by UDS within 1 h may persist in the AT cells for 4 h or longer.
D000293:Adolescent; D001260:Ataxia Telangiectasia / Q000378:metabolism*; D002478:Cells, Cultured; D002648:Child; D004247:DNA / Q000096:biosynthesis*; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D006801:Humans; D008297:Male; D010101:Oxygen Consumption
D004247:DNA