Purification and characterization of a lipolytic factor (toxohormone-L) from cell-free fluid of ascites sarcoma 180.
A lipolytic substance in the ascites fluid of mice with Sarcoma 180, called toxohormone-L, was purified and characterized. The lipolytic activity of toxohormone-L was measured in vitro using rat adipose tissue slices. Toxohormone-L, purified approximately 90-fold from the ammonium sulfate fraction, gave a single band on polyacrylamide gel electrophoresis. Its molecular weight was about 75,000, and its isoelectric point was 4.7. Toxohormone-L is heat labile and nondialyzable, but, on its digestion with trypsin, an active fragment that was heat stable and dialyzable was produced. Toxohormone-L is a protein and is also present in the ascites fluid of patients with hepatoma and Grawitz's tumor.
D000818:Animals; D004731:Endotoxins / Q000302:isolation & purification*; D005122:Exudates and Transudates / Q000032:analysis; D007526:Isoelectric Point; D008066:Lipolysis; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D012513:Sarcoma, Experimental / Q000032:analysis*; D014280:Triglycerides / Q000378:metabolism
D004731:Endotoxins; D009363:Neoplasm Proteins; D014280:Triglycerides; C010355:toxohormone