Prolylcarboxypeptidase (angiotensinase C) in human lung and cultured cells.
The activity of prolylcarboxypeptidase (PCP), or angiotensinase C, was measured in lung tissues, leukocytes, and cultured human cells using Cbz-Pro-[14C]Ala as a substrate. A lysosomal fraction of homogenized rat or human lung contained most of the PCP activity in that tissue. Polymorphonuclear neutrophils, macrophages, and lymphocytes isolated from human blood had PCP activity. Fibroblasts cultured from human tissues had the highest activity (0.56-1.15 mumol/h per 10(6) cells), more than endothelial cells cultured from human pulmonary arteries. PCP of cultured human fibroblasts was similar to the human renal enzyme because it was resistant to moderate heating and was not inhibited by p-chloromercuriphenyl sulfonic acid. These properties and the substrate specificity distinguish PCP from cathepsin A, which is also in fibroblasts. Antibody to human renal PCP reacted with fibroblast PCP in immunofluorescence, indicating common antigenic determinants. Hydrocortisone changed PCP activity in fibroblasts in parallel with changes in beta-glucuronidase activity and cell-protein concentration; the activity was depressed at low concentration of the hormone. PCP activity was also found in synovial fluid from arthritic joints and in fibroblasts from the synovium. That PCP is found in both inflammatory exudates and in cells that appear at sites of inflammation indicates that, in addition to inactivating angiotensins, this enzyme may have a role in inflammation.
D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D002268:Carboxypeptidases / Q000378:metabolism*; D002478:Cells, Cultured; D004727:Endothelium / Q000201:enzymology; D005347:Fibroblasts / Q000201:enzymology; D006098:Granulocytes / Q000201:enzymology; D006133:Growth Substances / Q000494:pharmacology; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D008168:Lung / Q000201:enzymology*; D008214:Lymphocytes / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology; D011392:Proline / Q000378:metabolism; D051381:Rats; D013582:Synovial Fluid / Q000201:enzymology
D006133:Growth Substances; D000804:Angiotensin II; D011392:Proline; D002268:Carboxypeptidases; C022448:lysosomal Pro-X carboxypeptidase; D006854:Hydrocortisone