Determination of urine and cerebrospinal fluid protein with the American Monitor KDA.
In turbidimetric measurements, true light absorbance is not measured but rather dispersion of light by particles in suspension causes a decrease in light energy to reach the detector. Reproducible, sensitive results can be obtained by controlling such parameters as timing, temperature, mixing of sample and reagents, and use of decrease wave-length. I have adapted two manual turbidimetric methods for the determination of the total protein in urine and cerebrospinal fluid to the American Monitor KDA. The method in precise and rapid, and correlates well with the two manual methods while using small sample and reagent volumes. The method is linear to at least 5.0 g/l.
D001322:Autoanalysis / Q000295:instrumentation; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D006801:Humans; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011507:Proteinuria / Q000652:urine*
D002556:Cerebrospinal Fluid Proteins