Micro-necrotic foci in regression of a murine calvarium transplantable tumor. 1. Cultivation and growth parameter of spontaneously transformed calvarium cell line.
The experiments reported in this communication describe the establishment of long-term cell lines, by in vitro cultivation of murine calvarium under: a) normal, physiological condition (Cont.Cal), where the cells were grown in standard Eagle's minimal essential medium (MEM), every 72 hours they were replenished with fresh medium and subcultured every 14 days, and b) experimental, non-physiological (Expt.Cal) condition, where the cells were grown in standard MEM, the cells were replenished with fresh medium every 28 days. During this period, and at weekly intervals the pH of the medium was readjusted to 7.3--7.5. These experimental cultures were subcultured once very 28 days at the time of feeding. Determinations of growth rate, requirement for serum growth factors, growth in soft agar, agglutination of the cells by lectin and their behavior on lectin columns, showed striking differences between the cells from the two conditions (Cont.Cal) and (Expt.Cal). It is suggested that either transformation or cellular selection occurred under the non-physiological conditions.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D003470:Culture Media; D051379:Mice; D009336:Necrosis; D009368:Neoplasm Transplantation; D012886:Skull / Q000166:cytology
D003470:Culture Media