title: A new procedure for the determination of transferrin C (Tf C) subtypes by isoelectric focusing. Existence of two additional alleles, Tf C4 and Tf C5.
abstract: Isoelectric focusing (IEF) is widely used for the determination of serum protein polymorphisms in forensic investigations. Because of non-genetic heterogeneity of some proteins and of protein-ligand dissociation observed after IEF standardization of procedures is necessary. In this article the authors point out the main difficulties encountered in studying the transferrin subtype polymorphism and describe a new and sensitive method. Two new Tf C subtypes were observed: Tf C4 in North American, Bolivian and Brazilian Indians, Tf C5 in Black Americans, both with polymorphic allele frequencies. In a Pyrenean population the allele frequencies for Tf C1, Tf C2 and Tf C3 were quite similar to those observed in a German population sample.
mesh_terms: D000483:Alleles; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D011110:Polymorphism, Genetic; D014168:Transferrin / Q000032:analysis / Q000235:genetics*
chemical_list: D014168:Transferrin