Isolation and characterization of lysozyme-sensitive mutants of Staphylococcus aureus.
Four lysozyme-sensitive mutants were isolated after nitrosoguanidine mutagenesis of a lysozyme-insensitive strain of Staphylococcus aureus. One mutant was sufficiently effective for the isolation of macromolecules, such as plasmid deoxyribonucleic acids, from a cell after lysozyme-induced cell lysis.
D001433:Bacteriolysis; D004269:DNA, Bacterial / Q000302:isolation & purification; D009113:Muramidase / Q000494:pharmacology*; D009154:Mutation; D010957:Plasmids; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics
D004269:DNA, Bacterial; D009113:Muramidase