A hemopoietic cell line dependent upon a factor in pokeweed mitogen-stimulated spleen cell conditioning medium.
A continuously growing cell line (FMP1.1) has been isolated, which is dependent upon a factor in PWCM for both growth and survival. FMP1.1 appears to be a mast cell, since IgE receptors are present and their granules react specifically with a mast cell granule stain. The factor in PWCM may be a glycoprotein and has biochemical properties in common with PWCM factors, which stimulate at least four other lineages of hemopoietic colony-forming cell. This line provides a pure population of cells as a model for studying molecular events of hemopoietic precursor cell growth and differentiation.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D006410:Hematopoiesis; D008297:Male; D008407:Mast Cells / Q000166:cytology*; D051379:Mice; D008811:Mice, Inbred DBA; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D013154:Spleen
D003470:Culture Media; D011043:Pokeweed Mitogens