Influence of sodium-independent gamma-aminobutyric acid binding on the assay of sodium-dependent gamma-aminobutyric acid binding in a membrane preparation of rat brain.
A large amount of [3H]GABA was bound to crude synaptic membrane fractions of rat, by sodium-independent process in a medium that contained 100 microM [3H]GABA used for assaying GABA uptake site. This [3H]-GABA binding was different from receptor binding of GABA. It was confirmed that this sodium-independent [3H]GABA binding scarcely occurred in the presence of a physiological concentration of sodium chloride, and that sodium-independent GABA binding had a negligible influence on sodium-dependent GABA binding.
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D001665:Binding Sites; D001921:Brain / Q000378:metabolism*; D002746:Chlorpromazine / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology*; D013570:Synaptic Membranes / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D012965:Sodium Chloride; D005680:gamma-Aminobutyric Acid; D002746:Chlorpromazine; D001640:Bicuculline