Fluorescence polarization studies of rat liver microsomes with altered phospholipid desaturase activities.
Phospholipid desaturase activity of rat liver microsomes can be varied by dietary manipulation: rats starved and refed a "fat-free" diet have two to three times the activity of normal controls whereas starved rats have no detectable activity. These changes were accompanied by changes in fatty acid composition of the microsomal phospholipids, resulting in a double bond: saturated fatty acid mole ratio (moles double bonds per mole saturated fatty acid) of 5.7 in control and starved rats and 4.7 in starved-refed rats. Fluorescence polarization ratio P (I parallel/I perpendicular to x instrument correction factor) of cis- and trans-parinaric acid (PnA) showed no significant differences in physical state of the three microsomal preparations. However, the isolated microsomal phospholipids with trans-PnA as probe showed differences in the temperature at which onset of a change in polarization ratio occurred (starved-refed greater than normal greater than starved rats). With the cis-PnA as probe, the polarization ratio showed no change in the range 10-40 degrees C but was significantly higher (1.8) in starved-refed rats than in normal and starved rats (1.6 in both cases). These data indicate that the microsomal phospholipids of starved-refed animals were in a less fluid state than those from control and starved rats and that this decrease in fluidity was correlated with an increase in phospholipid desaturase activity.
D000818:Animals; D002784:Cholesterol / Q000032:analysis; D003579:Cytochrome Reductases / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D007425:Intracellular Membranes / Q000201:enzymology; D007700:Kinetics; D008297:Male; D008563:Membrane Lipids / Q000502:physiology; D008862:Microsomes, Liver / Q000201:enzymology*; D010743:Phospholipids / Q000502:physiology; D051381:Rats; D013050:Spectrometry, Fluorescence; D013217:Starvation; D013379:Substrate Specificity; D013696:Temperature
D003580:Cytochromes; D005227:Fatty Acids; D008563:Membrane Lipids; D010743:Phospholipids; D002784:Cholesterol; C022369:phospholipid desaturase; D044943:Fatty Acid Desaturases; D003579:Cytochrome Reductases