Demonstration of a geometric isomer of bilirubin-IX alpha in the serum of a hyperbilirubinaemic newborn infant and the mechanism of jaundice phototherapy.
Photobilirubin-IX alpha in the serum of a hyperbilirubinaemic newborn infant was demonstrated for the first time by reversed-phase high-pressure liquid chromatography. The concentrations increased significantly during phototherapy. Corresponding to these changes in the serum, biliary unconjugated bilirubin concentrations increased markedly but photobilirubin-IX alpha was not detected in the bile. Moreover, large amounts of 'unknown pigment' that are diazo-negative appeared in the bile during phototherapy.
D001646:Bile / Q000378:metabolism; D001654:Bile Pigments / Q000378:metabolism; D001663:Bilirubin / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007231:Infant, Newborn; D007536:Isomerism; D007567:Jaundice, Neonatal / Q000097:blood* / Q000628:therapy; D010777:Photochemistry; D010789:Phototherapy
D001654:Bile Pigments; C028529:photobilirubin; D001663:Bilirubin