A simple procedure for the preparation of electrophoretically homogeneous alpha-actin from rabbit muscle.
A simplified procedure for the preparation of alpha-actinin from rabbit skeletal muscle is described. Use is made of the observation that actin is completely eliminated by denaturation, when myofibrils are dissolved in 0.6 M MgCl2 and thereafter MgCl2 is removed by dialysis.
D000185:Actinin / Q000302:isolation & purification*; D000818:Animals; D008274:Magnesium / Q000494:pharmacology; D009124:Muscle Proteins / Q000302:isolation & purification*; D009132:Muscles / Q000032:analysis*; D011489:Protein Denaturation / Q000187:drug effects; D011817:Rabbits
D009124:Muscle Proteins; D000185:Actinin; D008274:Magnesium