Nucleic acids and related enzymes. Secretion of four alkaline DNases by plasmodia of Physarum polycephalum.
In plasmodia of Physarum polycephalum, DNase activity with a preference for native DNA was found in a pattern of three or four isoenzymes. During growth a constant specific activity of approx. 0.3 unit of DNase activity per mg protein was found in the plasmodia, with a broad maximum during the G2-phase in the naturally synchronous flat cultures. Under conditions of starvation or sclerotization, DNase activity was secreted by the plasmodia in amounts which were up to ten times higher than the internal level of enzyme activity. Purification of the secreted DNase activity to high purity by three simple chromatographic steps showed that four different DNase isoenzymes existed which were identical with the intracellular ones. The relative abundances of the various isoenzyme forms inside and outside the plasmodia seemed to be slightly different. The possible functions of the DNase activities are discussed.
D002453:Cell Cycle; D003851:Deoxyribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism*; D010804:Physarum / Q000166:cytology / Q000201:enzymology*
D007527:Isoenzymes; D003851:Deoxyribonucleases