Preparation and characterization of inverted cell envelopes of Halobacterium halobium.
Inside-out cell envelopes from Halobacterium halobium R1M1 have been isolated by sonication and subsequent sucrose-density-gradient fractionation. The inverted cell envelopes transport protons from the exterior of the vesicles by a light-dependent, uncoupler-inhibited process. Enzymes usually facing the inside of the cell are exposed to the external medium in this preparation.
D001436:Bacteriorhodopsins / Q000378:metabolism; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D002499:Centrifugation, Density Gradient / Q000379:methods; D006217:Halobacterium / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light
D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D001436:Bacteriorhodopsins