Progesterone receptors: saturation analysis by a solid phase hydroxylapatite adsorption technique.
An assay for cytoplasmic progesterone receptor is described in which receptor is bound to hydroxylapatite and is washed before saturation analysis. Using 3H-progesterone as ligand, the assay yields lower, more reproducible nonspecific binding than is obtained with dextran-coated charcoal techniques and may provide a clinically useful method of measuring progesterone receptor in breast carcinoma. Nuclear progesterone receptors can also be determined by the same technique.
D001943:Breast Neoplasms / Q000032:analysis*; D002277:Carcinoma / Q000032:analysis*; D003600:Cytosol / Q000032:analysis; D005260:Female; D006801:Humans; D006882:Hydroxyapatites; D008722:Methods; D009214:Myoma / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*
D006882:Hydroxyapatites; D011980:Receptors, Progesterone