A microtitre plate radioimmunoassay for the detection and semiquantitation of housedust mite, rye grass pollen and cow's milk specific IgG antibodies.
A microtitre plate based radioimmunoassay (RIA) to measure semiquantitatively allergen specific IgA antibodies is described, with optimal coupling conditions for 3 allergens, house dust mite (Dermatophagoides pternoyssinus), rye grass pollen and cow's milk, and the optimal serum and [125I]anti-IgA incubation conditions.
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D004391:Dust; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology*; D008892:Milk; D008925:Mites; D011058:Pollen; D011863:Radioimmunoassay / Q000379:methods*
D000485:Allergens; D004391:Dust; D007070:Immunoglobulin A