Cortisol protection of the granulocyte response to isoproterenol during an in vitro influenza virus incubation.
Isolated human polymorphonuclear leukocytes (PMNs) release beta glucuronidase (BG) lysosomal enzyme during incubation with complement-activated zymosan particles. In asthma, isoproterenol (ISO) and histamine (HIS) inhibition of PMN lysosomal enzyme release is impaired while the prostaglandin E1 (PGE1) response is normal. During a respiratory infection provoking asthma, the PMN response to ISO is further impaired. The PMN response to ISO, HIS, and PGE1 is similarly impaired following an in vitro incubation with a live influenza bivalent (A + B) vaccine. In the following study, granulocytes were isolated from normal (n = 10) and asthma patients (n = 29). The isolated cells were incubated with the bivalent influenza vaccine (1 PMN:1 egg infective dose50) for 30 min. Following this in vitro virus incubation, granulocytes from normal and asthma patients had an imparied response to ISO, HIS, and PGE1. Granulocytes from asthma patients having a history of wheezing with respiratory illnesses did not have a greater impairment in the agonist response following virus incubation than normals or allergic asthma patients. However, in asthma patients (n = 6) receiving prednisone (10 to 20 mg/day), the in vitro vaccine incubation did not impair the PMN ISO response. A similar protective effect was found if granulocytes were incubated with hydrocortisone (1,4 X 10(-4) M) prior to exposure to the influenza vaccine.
D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone / Q000494:pharmacology; D000328:Adult; D005260:Female; D005966:Glucuronidase / Q000378:metabolism*; D006098:Granulocytes / Q000502:physiology*; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D007252:Influenza Vaccines / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009975:Orthomyxoviridae / Q000187:drug effects*; D011458:Prostaglandins E / Q000494:pharmacology; D015054:Zymosan / Q000494:pharmacology
D007252:Influenza Vaccines; D011458:Prostaglandins E; D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone; D006632:Histamine; D015054:Zymosan; D005966:Glucuronidase; D007545:Isoproterenol; D006854:Hydrocortisone