title: Evidence for a change in oestradiol negative feedback and LH pulse frequency in post-partum ewes.
abstract: Plasma LH levels and the effects of ovariectomy and of chronic and acute administration of oestradiol-17 beta were compared in post-partum (anoestrous) and cyclic Merino ewes during the breeding season. Plasma LH and LH pulses were detected in more post-partum (anoestrous) than cyclic (dioestrous) ewes (10/10 and 4/10 respectively). The post-partum ewes had a greater pulse frequency (1.8 +/- 0.42 compared with 0.4 +/- 0.16 pulses/6 h) and higher plasma LH levels (1.16 +/- 0.32 compared with 0.17 +/- 0.05 ng/ml). Ovariectomized post-partum ewes demonstrated fewer LH pulses (3.8 +/- 0.36 compared with 5.0 +/- 0.15 pulses/6 h) than did ovariectomized cyclic ewes. The depression of plasma LH following acute i.v. injection of 3 micrograms oestradiol-17 beta was similar (0.05 < P < 0.1) for the two groups (80.5 +/- 3.6 and 66.3 +/- 4.1%). At 7 days after ovariectomy and an oestradiol implant more cyclic ewes than post-partum ewes showed detectable plasma LH and LH pulses (6/10 compared with 1/9 ewes). By 66 h after implant removal pulse frequency was less in the post-partum ewes (3.2 +/- 0.55 compared with 5.5 +/- 0.56 pulses/6 h). These findings indicate that in post-partum ewes there is an increased inhibitory (negative-feedback) effect of oestradiol on LH release and a lower intrinsic frequency of pulsatile release of LH. These factors may be casually related to ovarian acyclicity.
mesh_terms: D000780:Anestrus; D000818:Animals; D002369:Castration; D004031:Diestrus; D004958:Estradiol / Q000494:pharmacology*; D005246:Feedback; D005260:Female; D007986:Luteinizing Hormone / Q000097:blood / Q000378:metabolism*; D049590:Postpartum Period; D011247:Pregnancy; D012636:Secretory Rate / Q000187:drug effects; D012756:Sheep / Q000502:physiology*
chemical_list: D004958:Estradiol; D007986:Luteinizing Hormone