Expression of blood group A antigens in human bone marrow cells.
We have studied the appearance of blood group A-activity during hematopoiesis in human bone marrow cells by the use of the blood group A-specific lectin from Vicia cracca. Cells that bound the lectin were identified using antiserum against the lectin followed by rosetting with protein A-containing Staphylococcus aureus cells. Only cells of the erythroid lineage from blood group A individuals formed staphylococcal rosettes. A-activity occurred in basophilic normoblasts and later stages of erythropoiesis, whereas pronormoblasts were negative. The appearance of blood group A-activity coincided roughly with the onset of hemoglobin synthesis and slightly later than the expression of the major sialoglycoprotein of erythrocytes, glycophorin A. Glycophorin A did not, however, contain blood group A-activity when analyzed by immunoprecipitation and gel electrophoresis.
D000017:ABO Blood-Group System; D000116:Acetylgalactosamine / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D001853:Bone Marrow / Q000276:immunology*; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D006454:Hemoglobins; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology; D011961:Receptors, Fc; D012397:Rosette Formation; D013211:Staphylococcus aureus; D013552:Swine
D000017:ABO Blood-Group System; D006454:Hemoglobins; D007106:Immune Sera; D037102:Lectins; D011961:Receptors, Fc; D000116:Acetylgalactosamine