Screening procedures for detection of hyperlipoproteinemia. I. Comparison of four turbidimetric methods for simultaneous determination of low- and very low-density lipoproteins.
In order to chose a reproducible and discriminatory method for use in mass screening for hyperlipoproteinemia, a comparison was made of four procedures of LDL and VLDL precipitation with polyanions in the presence of divalent cations. All the four methods (ie., heparin-Mn2+, heparin-Ca2+, heparin-Mg2+, and phosphotungstate-Mg2+) were applied simultaneously to aliquots from a single serum sample. Highly significant correlations were found when the results on 104 randomly selected sera were analyzed by matching every method with each of the other three techniques, indicating that all the four methods are really measuring the same analytes. The heparin-Mn2+ method, however, was more precise, selective and quantitative.
D006801:Humans; D006951:Hyperlipoproteinemias / Q000175:diagnosis*; D007122:Immunoelectrophoresis; D008077:Lipoproteins, LDL / Q000032:analysis; D008079:Lipoproteins, VLDL / Q000032:analysis; D008403:Mass Screening; D009391:Nephelometry and Turbidimetry
D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL