Identification and preliminary characterization of Vibrio cholerae outer membrane proteins.
Outer membrane proteins of Vibrio cholerae were purified by sucrose density centrifugation and Triton X-100 extraction at 10 mM Mg2+. The proteins were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. V. cholerae outer membrane proteins presented a unique pattern when compared with the patterns of other gram-negative rods. There were 8 to 10 major bands (Mr 94,000 to 27,000), with most of the protein located in band 5 (Mr approximately 45,000), which thus appears to be the major structural protein of the outer membrane. Lipid and carbohydrate were associated with band 6.
D001426:Bacterial Proteins / Q000032:analysis*; D002458:Cell Fractionation / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D008565:Membrane Proteins / Q000032:analysis*; D011092:Polyethylene Glycols; D014734:Vibrio cholerae / Q000032:analysis* / Q000254:growth & development / Q000648:ultrastructure
D001426:Bacterial Proteins; D008565:Membrane Proteins; D011092:Polyethylene Glycols