[Isolation and characterization of an acetylester-hydrolase from Aspergillus niger (author's transl)].
The characteristic features of an acetic acid esters hydrolyzing enzyme (acetylesterase, EC 3.1.1.16) are described. The pH- and temperature optimum were 7.0 and 40 degrees C respectively. The stability of the enzyme regarding different pH- and temperature conditions was investigated. The molecular weight of the acetylesterase could be determined to 160 000. A small acetic ester hydrolyzing activity was found too with a molecular weight of about 25 000. The activity was not inhibited by addition of di-isopropylphosphorofluoridate (DFP) or physostigmine. The KM-value for glyceryl triacetate was about 90 mM. Concentration of the enzyme was done by ultrafiltration and column-chromatography. The enzymatic activity tests were performed titrimetrically using glyceryl triacetate for substrate.
D000115:Acetylesterase / Q000302:isolation & purification / Q000378:metabolism*; D001234:Aspergillus niger / Q000201:enzymology*; D007531:Isoflurophate / Q000494:pharmacology; D007700:Kinetics; D008970:Molecular Weight; D013379:Substrate Specificity
D007531:Isoflurophate; D000115:Acetylesterase
Isolierung und Charakterisierung einer Acetylester-Hydrolase aus Aspergilus niger.