[Obtaining mutants that yield tobramycin in a culture of the producer of a complex of aminoglycoside antibiotics, Streptomyces cremeus var. tobramycini var. nov. 2242].
The organism producing an aminoglycoside antibiotic complex was subjected to selection with a purpose of obtaining a strain producing tobramycin. Spontaneous and gamma-radiation-induced variation of the strains obtained as a result of a step-wise selection of the tobramycin-producing organism was studied. Correlation between the antibiotic activity levels and morphological features of these strains was shown. 8 physiological groups of mutants were differentiated and characterized as dependent on the ratio of the components in the aminoglycoside antibiotic complex produced by them. A highly productive strain 3406 was selected. The level of tobramycin synthesis by this strain is 10 times higher than that of the initial culture.
D000617:Aminoglycosides / Q000096:biosynthesis*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D005720:Gamma Rays; D014644:Genetic Variation / Q000528:radiation effects; D009154:Mutation; D012641:Selection, Genetic; D012988:Soil Microbiology; D013302:Streptomyces / Q000378:metabolism* / Q000528:radiation effects; D014031:Tobramycin / Q000096:biosynthesis*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D014031:Tobramycin
Poluchenie mutantov, obrazuiushchikh tobramitsin, v kul'ture produtsenta kompleksa aminoglikozidnykh antibiotikov Streptomyces cremeus var. tobramycini var. nov. 2242.