title: In vitro induction of ornithine decarboxylase in urinary bladder carcinoma cells.
abstract: The induction of ornithine decarboxylase by normal rat urine in bladder cancer cell cultures was tested in view of recent observations that urine acts as a tumor promoter. Addition of urine up to 15% in final concentration to culture medium resulted in a 10-fold increase in ornithine decarboxylase activity over the control. The stimulatory factor(s) contained in urine appears heat stable and may be multiple. 12-O-Tetradecanoylphorbol-13-acetate, a potent promoter in mouse skin carcinogenesis, induced a 39-fold increase in ornithine decarboxylase activity, the best response among the various substances tested. This suggests that it may act as a promoter of bladder cancer.
mesh_terms: D000818:Animals; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D002273:Carcinogens / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000201:enzymology; D002295:Carcinoma, Transitional Cell / Q000201:enzymology; D002460:Cell Line; D003043:Cocarcinogenesis; D004790:Enzyme Induction / Q000187:drug effects; D009374:Neoplasms, Experimental / Q000201:enzymology; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D001749:Urinary Bladder Neoplasms / Q000201:enzymology* / Q000209:etiology; D014556:Urine / Q000502:physiology
chemical_list: D002273:Carcinogens; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D013755:Tetradecanoylphorbol Acetate