Quantification of damage to striated muscle after normothermic or hypothermic ischemia.
Muscular ischemia of different duration was induced by use of a tourniquet applied to the rear left leg of rabbits under normothermic or hypothermic conditions, and variations in serum creatine kinase, lactate dehydrogenase, K+, Ca2+, and lactate were determined at various intervals after blood flow was restored. A monocompartmental model analysis applied to the activities of the two enzymes in the serum demonstrated that: (a) the total enzyme activity leaked into the blood is proportional to the duration of normothermic but not of hypothermic ischemia; (b) this enzyme leakage persists until at least the third day after blood flow is restored; (c) a substantial part of the ischemic damage seems to occur as a consequence of re-perfusion, not of the ischemia itself; and (d) hypothermia greatly minimizes the ischemic damage. The data we obtained for K+ and for lactate confirm the protective effect of hypothermia.
D000818:Animals; D002118:Calcium / Q000097:blood; D003402:Creatine Kinase / Q000097:blood; D004195:Disease Models, Animal; D007511:Ischemia / Q000201:enzymology / Q000503:physiopathology*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D009132:Muscles / Q000098:blood supply* / Q000201:enzymology / Q000503:physiopathology; D011188:Potassium / Q000097:blood; D011817:Rabbits; D013696:Temperature
D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D011188:Potassium; D002118:Calcium