Differences between adrenal adenoma causing primary aldosteronism and other adrenal tissues in the incorporation of labeled steroid precursors into their products.
The incorporation and conversion of several labeled steroid precursors into their products were examined in slices of adrenal tissue from two patients with primary aldosteronism and compared with that in "normal" adrenal tissue and adrenal tissues from a patient with Cushing's syndrome. The products of the incorporation were separated by Sephadex LH-20 column chromatography. The major products of conversion in the adenomatous tissue of primary aldosteronism were 18-hydroxycorticosterone and lesser amounts of aldosterone. Smaller amounts of 18-hydroxycorticosterone were isolated from all other adrenal tissues studied. No aldosterone could be recovered after incubating any of the adrenal tissue studied with labeled 18-hydroxy-11-deoxycorticosterone or 18-hydroxycorticosterone as precursor steroid. These in vitro results seem to suggest that there is increased 18-hydroxylation in the adenoma of primary aldosteronism compared with other tissues and that relatively more 18-hydroxycorticosterone is produced in such tissue than aldosterone.
D000236:Adenoma / Q000150:complications / Q000378:metabolism*; D000310:Adrenal Gland Neoplasms / Q000150:complications / Q000378:metabolism*; D000311:Adrenal Glands / Q000378:metabolism*; D003480:Cushing Syndrome / Q000378:metabolism; D006801:Humans; D006929:Hyperaldosteronism / Q000209:etiology / Q000378:metabolism*; D066298:In Vitro Techniques; D013256:Steroids / Q000378:metabolism*
D013256:Steroids