Photokinetic microanalysis of NADP+, using bacterial luciferase.
Bioluminescence photokinetic assay of NADP+ is described, using the glucose-6-phosphate dehydrogenase reaction for conversion to its reduced form and subsequent measurement of this with luciferase extracts of Vibria fisherii. the analyses were applied to the determination of the activity of minute amounts of glutathione reductase using NADP+ as measurable product and for nucleotide assay in cell samples of 0.5--10 microgram dry weight. The sensitivity was sufficient for determining 0.5 picomoles NADP+. Previously, FMN, NADH, NAD+ and NADH have been analysed with the bacterial luciferase system. Its applicability has not been extended by the assay of NADP+.
D004798:Enzymes / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007700:Kinetics; D008156:Luciferases; D009249:NADP / Q000378:metabolism*; D010776:Photobacterium / Q000201:enzymology; D010783:Photometry / Q000379:methods*
D004798:Enzymes; D009249:NADP; D008156:Luciferases; D005980:Glutathione Reductase