Formation of purple hemocyanin upon addition of ethyleneglycol to Sepioteuthis lessoniana oxyhemocyanin.
We report the formation of purple hemocyanin upon addition of ethyleneglycol to a solution of Sepioteuthis lessoniana oxyhemocyanin in 0.05 M Tris-HCl buffer (pH 7.5). Spectroscopic studies on the purple hemocyanin indicate that this transformation between the normal blue state and the purple state of oxyhemocyanin is essentially a reversible one. The purple hemocyanin exhibits esr signals that correspond to about one-fifth of the total copper obtained. The results suggest that ethyleneglycol causes a moderate and reversible deformation of the active site of the oxyhemocyanin.
D000818:Animals; D049832:Decapodiformes; D005026:Ethylene Glycols; D006433:Hemocyanins; D010084:Oxidation-Reduction; D010100:Oxygen; D013053:Spectrophotometry
D005026:Ethylene Glycols; D006433:Hemocyanins; D010100:Oxygen