Incomplete separation of radioiodinated thyroid hormones in serum using specific antibodies.
Alkaline Sephadex G-25 columns were used to separate labelled 3,5,3',5'-thyroxine, 3,5,3'-triiodothyronine, 3,3',5'-triiodothyronine and 3,3'-diiodothyronine from the serum binding proteins followed by a quantitative elution of each hormone by coupling to its respective antibody. It is shown that although these antibodies (diluted 1:1500-1:100 000) in our radioimmunoassays are highly specific they show a high degree of non-specific binding when they are used in the concentrations necessary to get a maximal recovery of the hormones in column separating experiments.
D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001798:Blood Proteins / Q000032:analysis; D002850:Chromatography, Gel; D003429:Cross Reactions; D004104:Diiodothyronines / Q000302:isolation & purification*; D006801:Humans; D011863:Radioimmunoassay; D013970:Thyronines / Q000302:isolation & purification*; D013974:Thyroxine / Q000302:isolation & purification*; D014284:Triiodothyronine / Q000302:isolation & purification*; D014285:Triiodothyronine, Reverse / Q000302:isolation & purification*
D001798:Blood Proteins; D004104:Diiodothyronines; D013970:Thyronines; D014284:Triiodothyronine; D014285:Triiodothyronine, Reverse; D013974:Thyroxine