Discrimination between spermine and calcium binding activities in crude extracts from chick duodenal mucosa.
Chick duodenum contains proteins capable to bind radioactive spermine and calcium. From competition studies the spermine binding protein(s) seem(s) to differ from calcium binding protein. This finding makes possible to distinguish and assay separately those two cation binding activities.
D000818:Animals; D001667:Binding, Competitive; D002118:Calcium / Q000378:metabolism*; D002645:Chickens; D004386:Duodenum / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism; D011402:Pronase / Q000494:pharmacology; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D013096:Spermine / Q000378:metabolism*; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology
D064030:S100 Calcium Binding Protein G; D013096:Spermine; D014357:Trypsin; D011402:Pronase; D002118:Calcium