Direct observation of reduction of met- and ferrylmyoglobins in the hemoglobin-free perfused rat heart.
It was demonstrated spectrophotometrically that in the hemoglobin-free perfused rat heart met- and ferrylmyoglobins formed upon infusion of sodium nitrite, and ethyl hydroperoxide were reduced to form oxymyoglobin with a half time of about 3 and 4 min, respectively. The rate of metmyoglobin reduction was about 30 nmol/min/g wet weight in the rat heart.
D000818:Animals; D006420:Hemeproteins / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008786:Metmyoglobin / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D010084:Oxidation-Reduction; D010477:Perfusion; D051381:Rats; D013053:Spectrophotometry
D006420:Hemeproteins; D006454:Hemoglobins; C028048:ferrylmyoglobin; D008786:Metmyoglobin