Micro-determination of inorganic phosphorus and alkaline phosphatase activity in biological fluids.
Inorganic phosphorus and alkaline phosphatase activities were measured in serum samples from goats, buffaloes, cows, bullocks and also in amniotic and allantoic fluid of goats using ascorbic acid as reductant for phosphomolybdate. The results were compared with conventional method of FISKE and SUBBAROW (1925). There were no significant differences between the 2 methods. It was possible to measure micro-amounts of inorganic phosphorus and alkaline phosphatase activity with precision even in low cost colorimeters.
D000469:Alkaline Phosphatase / Q000032:analysis* / Q000097:blood; D000482:Allantois; D000653:Amniotic Fluid / Q000032:analysis / Q000201:enzymology; D000818:Animals; D001826:Body Fluids / Q000032:analysis* / Q000201:enzymology; D002020:Buffaloes / Q000378:metabolism; D002417:Cattle / Q000378:metabolism; D003124:Colorimetry; D005260:Female; D006041:Goats / Q000378:metabolism; D008297:Male; D010758:Phosphorus / Q000032:analysis* / Q000097:blood; D011247:Pregnancy; D013055:Spectrophotometry, Infrared / Q000379:methods*
D010758:Phosphorus; D000469:Alkaline Phosphatase