Differential effects of nitrated ricin and nitrated and dithionite-reduced ricin on protein-synthesis inhibition and transmembrane tramsport in eukaryotic cells.
Ricin, was nitrated with tetranitromethane and reduced with sodium dithionite. Of the 8.0 nitro groups incorporated, 3.2 were on the A chain and 5.1 were on the B chain. Nitrated ricin1 was somewhat less active than nitrated and reduced ricin1 in inhibiting protein synthesis in vitro, but both were highly inhibitory. However, the modified toxins were less than 1% as active as ricin in inhibiting protein synthesis in cultured cells. Indirect immunofluorescence assays demonstrated tha both modified toxins were specifically bound to the cell surface and could be displaced by galactose.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002477:Cells / Q000378:metabolism*; D002478:Cells, Cultured; D004227:Dithionite; D005057:Eukaryotic Cells / Q000187:drug effects / Q000378:metabolism*; D007202:Indicators and Reagents; D051379:Mice; D009566:Nitrates; D014176:Protein Biosynthesis; D012276:Ricin / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013774:Tetranitromethane
D007202:Indicators and Reagents; D009566:Nitrates; D004227:Dithionite; D012276:Ricin; D013774:Tetranitromethane