title: The indirect haemolysis test (IHLT) for bovine brucellosis - comparisons with the complement fixation test (CFT) in vaccinated and experimentally infected cattle.
abstract: Cattle were vaccinated with Brucella abortus strain 19 subcutaneously in doses ranging from the normal (2.0 ml) dose of standard vaccine down to 1/400 of the normal dose, and via the conjunctival sac with 1/2 of 1/20 of the normal dose. Under all vaccination regimes serum antibody titres in the complement fixation test (CFT) rose more rapidly, reached higher levels, declined more slowly and involved a greater proportion of animals, than titres in the indirect haemolysis test (IHLT). The interval between the first positive serological test and parturition was determined for 54 pregnant cows infected with a virulent field strain (VRI 3) of B. abortus. On average the CFT titre rose to 1/4 43 days, and 1/8 33 days, before parturition, while the IHLT reached a 2/8 reaction 31 days before parturition.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D002007:Brucellosis, Bovine / Q000276:immunology*; D002417:Cattle; D003168:Complement Fixation Tests / Q000662:veterinary*; D005260:Female; D006461:Hemolysis; D014611:Vaccination / Q000662:veterinary*
chemical_list: D000907:Antibodies, Bacterial