Recognition of a positive MLC reaction after four hours.
EMT indicator cells were incubated for 1 h in the supernatant of a 3 h MLC. Their electrophoretic mobility was then measured by an analytical, carrier-free electrophoresis system. The alteration of their mobility against a medium-standard was calculated in per cent and correlated with the conventional measured MLR-cpm. According to our results the correlation of the two quantities is statistically highly significant. This method could enable recognition of a positive or negative MLC after only 4 h. This fact could be of great importance for histocompatibility testing and transplantation.
D002465:Cell Movement; D004586:Electrophoresis; D006650:Histocompatibility Testing; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes; D008433:Mathematics; D013997:Time Factors; D014181:Transplantation Immunology