CSF putrescine levels. The enzymatic-isotopic method vs liquid chromatography.
Twenty-six samples of CSF were assayed for their putrescine content by three analytical techniques: total and free putrescine levels were assayed by fluorometry after separation by column chromatography and compared with putrescine levels as assayed by the enzymatic-isotopic method. Putrescine levels obtained by the enzymatic-isotopic method correlated relatively well with free putrescine levels, but grossly underestimated the results obtained for total putrescine values. The difference between total and free putrescine values is mostly due to metabolites of putrescine, such as monoacetylputrescine, which account for about 40% of the total putrescine value. The importance of the free putrescine level as a marker of CNS malignant neoplasms in comparison to that of the total putrescine level deserves investigation.
D001932:Brain Neoplasms / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D002852:Chromatography, Ion Exchange; D004796:Clinical Enzyme Tests; D006801:Humans; D011700:Putrescine / Q000134:cerebrospinal fluid*
D011700:Putrescine