Incorporation of 14C-mevalonate into biliary cholesterol and bile acids by perfused rat liver: effect of plasma and individual lipoproteins.
Effect of plasma and individual lipoproteins on the incorporation of 14C-mevalonate into biliary bile acids and cholesterol by perfused rat liver was investigated. Use of plasma free perfusate (instead of whole blood) gave similar rates of incorporation of 14C-mevalonate into biliary bile acids, but showed a decrease in percent distribution in cholic acid and a increase in beta-muricholic acid. Addition of VLDL (isolated from Zucker rats) into the plasma free perfusate caused a significant increase in the incorporation of the label into bile acids, but LDL and HDL had no effect. HDL caused an increase in biliary excretion of 14C-cholesterol.
D000818:Animals; D001646:Bile / Q000187:drug effects / Q000378:metabolism; D001647:Bile Acids and Salts / Q000096:biosynthesis*; D002784:Cholesterol / Q000096:biosynthesis*; D008074:Lipoproteins / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000494:pharmacology; D008079:Lipoproteins, VLDL / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008798:Mevalonic Acid / Q000378:metabolism*; D010477:Perfusion; D051381:Rats
D001647:Bile Acids and Salts; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008079:Lipoproteins, VLDL; D002784:Cholesterol; D008798:Mevalonic Acid