Deuterium oxide protects against hyperthermic cell death: evidence for role of interfacial water.
Tissue culture cells incubated at 43 degrees C show a dramatic decrease in cell survival compared with cells incubated at lower temperatures; the effect is termed hyperthermic cell death (HCD). Replacing water in the culture medium with deuterium oxide produces a dose-dependent reduction in HCD. On the basis of this and other evidence it is concluded that the mechanism of HCD involves a hydration-dependent phase transition in membrane lipids.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D006224:Cricetinae; D003903:Deuterium; D004305:Dose-Response Relationship, Drug; D006358:Hot Temperature; D008563:Membrane Lipids; D010743:Phospholipids; D014867:Water
D008563:Membrane Lipids; D010743:Phospholipids; D014867:Water; D003903:Deuterium