Preparation of rabbit alveolar macrophages in high purity and yield.
We describe methods that permit large numbers of viable and functional alveolar macrophages (greater than or equal to 5 x 10(8)/rabbit), substantially free of granulocyte contamination, to be recovered. Such populations are especially well suited for biochemical analyses demanding cell purity.
D000818:Animals; D002469:Cell Separation; D005620:Freund's Adjuvant / Q000008:administration & dosage; D008168:Lung / Q000166:cytology*; D008264:Macrophages; D010587:Phagocytosis; D011817:Rabbits
D005620:Freund's Adjuvant