Platelet functions and fatty acid composition of platelet phospholipids in spontaneously hypertensive rats fed saturated or polyunsaturated fats.
Spontaneously hypertensive rats (Okamoto-Wistar) as compared to their normotensive controls (Kyoto-Wistar) presented a markedly higher platelet activity both in coagulation (as evaluated by the recalcification plasma clotting time of platelet-rich plasma) and aggregation, as triggered by thrombin. By comparing animals fed saturated or polyunsaturated fat, it could be observed that the saturated fat diet induced similar results on platelet functions to these observed in hypertension. In addition, the saturated fat diet further increased the platelet response of the hypertensive animals. By contrast, the polyunsaturated fat, could neither reduce the hypertension nor completely abolish the platelet reactivity associated with the hypertension. The most significant change induced by the saturated fat diet in the fatty acid composition of the platelet phospholipids was an increase in 20:3 omega 9, further enhanced in the hypertensive animals. In the polyunsaturated diet-fed rats, it was mostly 20:4 which was more elevated in the platelet phospholipids of the hypertensive. As a result, it was the sum of 20:3 omega 9 + 20:4 in the platelet phospholipids, which appeared to be the most significantly related, in the 4 groups of animals, to the response of platelets to thrombin induced aggregation.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D001792:Blood Platelets / Q000503:physiopathology*; D001794:Blood Pressure / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D005224:Fats, Unsaturated / Q000008:administration & dosage; D005227:Fatty Acids / Q000008:administration & dosage / Q000097:blood*; D006973:Hypertension / Q000503:physiopathology*; D010743:Phospholipids / Q000097:blood*; D010974:Platelet Aggregation / Q000187:drug effects; D051381:Rats; D013997:Time Factors; D014280:Triglycerides / Q000097:blood
D005224:Fats, Unsaturated; D005227:Fatty Acids; D010743:Phospholipids; D014280:Triglycerides; D000244:Adenosine Diphosphate; D002784:Cholesterol