title: Quantitative determination of carcinogens and mutagens as alkylating agents following chemical activation.
abstract: Reaction of nitrosodiethylamine (NDEA) in a non-enzymatic, chemical activation system consisting of ascorbic acid, ferrous ions, EDTA and molecular oxygen yielded an alkylating intermediate that was detected by its reaction with 4-(p-nitrobenzyl)pyridine (PNBP) to yield a colored product. Reaction conditions for the chemical activation-detection system were optimized, allowing detection of approx. 10 mumol NDEA. The method detected a variety of acyclic and cyclic nitrosamines and other compounds that become alkylating agents only following oxidative metabolism.
mesh_terms: D000477:Alkylating Agents / Q000032:analysis* / Q000378:metabolism; D001711:Biotransformation; D002273:Carcinogens / Q000032:analysis / Q000378:metabolism*; D009153:Mutagens / Q000032:analysis / Q000378:metabolism*; D009578:Nitrobenzenes; D009602:Nitrosamines / Q000032:analysis / Q000378:metabolism; D011725:Pyridines
chemical_list: D000477:Alkylating Agents; D002273:Carcinogens; D009153:Mutagens; D009578:Nitrobenzenes; D009602:Nitrosamines; D011725:Pyridines; C008633:4-(4-nitrobenzyl)pyridine