Visualization of rat brain glycoproteins in polyacrylamide gels by means of concanavalin A-peroxidase.
A reliable method for visualization of mannose-containing glycoproteins in electrophoretically (SDS-PAGE) separated Tris-soluble, Triton-soluble and Triton-resistant fractions obtained from rat hippocampus, brain region supposed to play an essential role in learning and memory processes, was elaborated. The method is based on the Concanavalin A-horseradish peroxidase reaction applied successfully for the purpose of light and electron microscopic visualization of cellular carbohydrate components.
D000818:Animals; D003208:Concanavalin A; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D006624:Hippocampus / Q000032:analysis* / Q000648:ultrastructure; D006651:Histocytochemistry / Q000379:methods*; D006735:Horseradish Peroxidase; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051381:Rats
D006023:Glycoproteins; D009419:Nerve Tissue Proteins; D003208:Concanavalin A; D006735:Horseradish Peroxidase