The quality assurance of antiglobulin reactions with Fab-sensitized reagent red blood cells.
The production, function, and use of a new antiglobulin control cell is described. The cells are sensitized with monovalent Fab (fragment, antigen-binding) fragments and can be agglutinated by active antiglobulin serum only. In contrast to commercial antiglobulin control cells, which are frequently associated with false-positive results, the reactivity of the Fab-sensitized cells is entirely specific. It is concluded that Fab-sensitized cells provide greater quality assurance than currently available reagents.
D001787:Blood Group Incompatibility / Q000209:etiology; D003298:Coombs Test; D004912:Erythrocytes; D005189:False Positive Reactions; D006386:Hemagglutination Tests; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D009994:Osmolar Concentration; D011786:Quality Control
D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G