[Determination of the activities of lipoprotein lipase, triacylglycerolipase, monoacylglycerolipase, and tributyrinase in the plasma, myocardium, and cardiac adipose tissue of rabbits].
Fatty emulsion intralipid, activated by rabbit blood plasma, intralipid, micellar emulsion of monooleine and tributyryne emulsion were used, respectively, as substrates for estimation of asctivities of lipoprotein lipase (EC 3.1.1.34), triacyl glycerolipase (EC 3.1.1.3), monoacyl glycerolipase (EC 3.1.1.23) and tributyrynase (EC 3.1.1.13) by modified Dol's method in rabbit heart tissue, heart fatty tissue and blood plasma. Effect of heparin on extraction of lipolytic enzymes from tissues was studied.
D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006493:Heparin / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000378:metabolism*; D008071:Lipoprotein Lipase / Q000378:metabolism; D008722:Methods; D008994:Monoacylglycerol Lipases / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology*; D011817:Rabbits
D006493:Heparin; D008994:Monoacylglycerol Lipases; D008049:Lipase; D008071:Lipoprotein Lipase; D002122:Calcium Chloride
Opredelenie aktivnosti lipoproteidlipazy, triatsilglitserolipazy, monoatsilglitserolipazy i tributirinazy v plazme krovi, miokarde i zhirovoĭ tkani serdtsa krolikov.