A footpad weight assay method to evaluate delayed-type hypersensitivity in the mouse.
Delayed-type hypersensitivity lesions have been difficult to evaluate objectively in the mouse. They are usually evaluated by measurement of footpad swelling or increased ear thickness after challenge with antigen. I have developed a footpad weight assay method which reduces the observer's error and the instrumental error. This method is reliable for the assessment of delayed-type hypersensitivity reactions when results are expressed as weight increase or weight ratio calculated on challenged and unchallenged footpad.
D000818:Animals; D005260:Female; D005528:Foot / Q000033:anatomy & histology*; D006968:Hypersensitivity, Delayed / Q000175:diagnosis*; D051379:Mice; D008813:Mice, Inbred ICR; D009929:Organ Size; D012756:Sheep; D013997:Time Factors