Specific and sensitive radioimmunoassay for 3-methoxy-4-hydroxyphenylethyleneglycol (MOPEG).
Antibodies that specifically bind the norepinephrine metabolite 3-methoxy-4-hydroxyphenylethyleneglycol (MOPEG) were produced in rabbits after injection of a derivative of MOPEG conjugated with bovine thyroglobulin. A sensitive radioimmunoassay was devised with this antiserum, in which as little as 0.5 nanogram of MOPEG can be accurately measured with a final antibody dilution of 1:180. The antibody appears to be specific for MOPEG, since tritiated MOPEG was not displaced from the antibodies by norepinephrine, epinephrine, dopamine, serotonin, or their major metabolites including MOPEG-sulfate (333 nanograms each).
D000818:Animals; D000918:Antibody Specificity; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping; D006018:Glycols / Q000032:analysis*; D008734:Methoxyhydroxyphenylglycol / Q000032:analysis* / Q000378:metabolism; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D013954:Thyroglobulin
D006018:Glycols; D008734:Methoxyhydroxyphenylglycol; D013954:Thyroglobulin