[The alkaline phosphatase activity in the lyophylized commercial control sera after reconstitution].
Soon after the reconstitution of the lyophylized commercial control sera the alkaline phosphatase activity is smaller than stated value and theoretical value is reached after a time varying according to the various sera; at any rate this value is hever higher than seven hours since reconstitution. The lyophylized sera reconstitution with a solution of diethanolamine 1 mol/l or MgCl2 0,5 mmol/l alone or associate consents to restore much more swiftly the enzymatic activity to a higher value than 90% of the stated value since reconstitution. No alkaline phosphatase variation is observed in the congealed human sera at --20 degrees C after the decongealment. We recommend to reconstitute the lyophylized sera for the alkaline phosphatase determination by the use of a solution of diethanolamine 1 mol/l and MgCl2 0,5 mmol/l instead of distilled water.
D000469:Alkaline Phosphatase / Q000097:blood*; D001793:Blood Preservation; D004355:Drug Stability; D004983:Ethanolamines; D005612:Freeze Drying; D006801:Humans; D007700:Kinetics; D008274:Magnesium; D015636:Magnesium Chloride; D011786:Quality Control; D012996:Solutions
D004983:Ethanolamines; D012996:Solutions; D015636:Magnesium Chloride; C020283:diethanolamine; D000469:Alkaline Phosphatase; D008274:Magnesium
Attività della fosfatasi alcalina nei sieri liofilizzati dopo ricostituzione.