Effect of apolipoproteins on the induction of hepatic steatosis in rats.
The incorporation of apolipoprotein E isolated from human very low density lipoproteins on a triglyceride emulsion produced a substantial increment in hepatic triglyceride after 1 h of in vitro perfusion through the isolated liver of a fasted rat. Both gross and microscopic morphology confirmed a substantial steatosis. Perfusions with triglyceride emulsions which contained no apoipoproteins resulted in a modest increment in hepatic triglyceride although considerably less than emulsions with the E protein, and no morphologic features of steatosis. The steatosis induced in the in vitro perfused fasted livers by the emulsions with E protein could be prevented when CIII-1 apoprotein was also incorporated on the emulsion. A fed rat liver perfused with the E-enriched emulsion accumulated significantly less triglyceride than the fasted preparation. These data suggest that the apoprotein patterns of the plasma triglyceride-rich lipoproteins are of considerable importance for the partition of triglyceride between liver and plasma.
D000818:Animals; D053305:Apolipoprotein C-III; D001053:Apolipoproteins / Q000009:adverse effects / Q000378:metabolism* / Q000494:pharmacology; D001056:Apolipoproteins C; D001057:Apolipoproteins E; D005215:Fasting; D005234:Fatty Liver / Q000139:chemically induced* / Q000473:pathology; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010477:Perfusion; D051381:Rats; D014280:Triglycerides / Q000378:metabolism
D053305:Apolipoprotein C-III; D001053:Apolipoproteins; D001056:Apolipoproteins C; D001057:Apolipoproteins E; D014280:Triglycerides