Purification of peptides: an efficient procedure for the separation of peptides from amino acids and salt.
A novel method for the separation of peptides from amino acids and salts has been developed. Separation is achieved by passage of the sample through a column of octadecasilyl-silica (ODS). Peptides, but not amino acids and salts, are strongly retained by ODS in an aqueous mobile phase and can be recovered by elution with an aqueous-alcoholic mobile phase. The method is applicable to most peptides and is fast and simple. Moreover, it is characterized by good recovery yields and high sample capacity.
D000596:Amino Acids / Q000302:isolation & purification*; D002850:Chromatography, Gel / Q000379:methods; D006728:Hormones / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D012492:Salts
D000596:Amino Acids; D006728:Hormones; D010455:Peptides; D012492:Salts