Immunoelectgrophoretic pattern of Klebsiella O and K antigens.
Klebsiella capsular antigens K1-K72 and O antigens O1-O9 were isolated and rabbits immunized with corresponding bacteria. The immunoelectrophoretic patterns of the O and K antigens were studied. All K antigens analyzed were highly electrophoretically mobile, giving a marked precipitate of different lengths towards the anode. All the O antigens analyzed with the exception of O4 were nonmobile and gave a precipitate next to the start basin. The O4 precipitated towards the anode. These data indicate a possibility for using counterimmunoelectrophoresis in the diagnosis of Klebsiella antigen in body fluids or for capsular typing of the bacteria using the same technique.
D000818:Animals; D000942:Antigens, Bacterial; D011232:Chemical Precipitation; D003378:Counterimmunoelectrophoresis; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D007709:Klebsiella / Q000276:immunology*; D011817:Rabbits
D000942:Antigens, Bacterial