Liquid-chromatographic assay for urinary homovanillic acid, with fluorescent detection.
We describe a procedure for measuring urinary homovanillic acid by "high-performance" liquid chromatography. The pH of urine samples is adjusted, and they are chromatographed directly on an octyl silica column. The effluent is reacted with a ferricyanide reagent, to convert homovanillic acid to a product that is fluorescent at 420 nm on excitation at 320 nm. The standard curve is linear up to a homovanillic acid concentration of 20 mg/L; the detection limit is 0.3 mg/L. The method is evaluated for precision, recovery, potential interference, and reference values, and compared (r = 0.99) with a published spectrophotometric method. The proposed method is suitable for the routine analysis for homovanillic acid in urine.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006719:Homovanillic Acid / Q000652:urine*; D006801:Humans; D009447:Neuroblastoma / Q000652:urine; D010648:Phenylacetates / Q000652:urine*; D012016:Reference Values; D013050:Spectrometry, Fluorescence / Q000379:methods
D010648:Phenylacetates; D006719:Homovanillic Acid