title: Rubella-specific IgM detection using Sephacryl S-300 gel filtration.
abstract: Sephacryl S-300 has been compared with Sephadex G-200 in the fractionation of sera for the detection of rubella-specific IgM. No difference in sensitivity was found with sera that had low titres of rubella-specific IgM. Sephadex G-200 was apparently more sensitive with some sera which had high titres of rubella-specific IgM due to the contribution of rubella-specific IgA. Sephacryl S-300 offers considerable advantages in ease and speed of preparation, and as it is a more rigid gel higher flow rates can be obtained. Sera may be fractionated in 2.5 hours on Sephacryl S-300 compared with 7-10 hours on Sephadex G-200.
mesh_terms: D000918:Antibody Specificity; D002850:Chromatography, Gel / Q000379:methods; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D012409:Rubella / Q000276:immunology; D012412:Rubella virus / Q000276:immunology*
chemical_list: D007075:Immunoglobulin M