Desaturation of linoleic acid from exogenous lipids by isolated chloroplasts.
When [14C]diacylgalactosylglycerol was added to isolated pea or lettuce chloroplasts linolenate synthesis was seen. The desaturation of [14C]linoleate in diacylgalactosylglycerol to [14C]linolenate was stimulated by the addition of a soluble protein fraction containing lipid-exchange activity. Other [14C]acyl lipids were ineffective, except that [14C]phosphatidylcholine in the presence of UDP-galactose and sn-glycerol 3-phosphate could also supply [14C]linoleate for desaturation. These results are consistent with a role of diacylgalactosylglycerol in linolenate synthesis, as indirectly suggested by labelling experiments.
D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D004075:Diglycerides / Q000378:metabolism*; D007887:Fabaceae; D038983:Galactolipids; D005989:Glycerides / Q000378:metabolism*; D005994:Glycerophosphates / Q000494:pharmacology; D006017:Glycolipids / Q000378:metabolism*; D008041:Linoleic Acids / Q000378:metabolism*; D008042:Linolenic Acids / Q000096:biosynthesis; D010713:Phosphatidylcholines / Q000378:metabolism*; D010946:Plants, Medicinal; D014531:Uridine Diphosphate Galactose / Q000494:pharmacology
D004075:Diglycerides; D038983:Galactolipids; D005989:Glycerides; D005994:Glycerophosphates; D006017:Glycolipids; D008041:Linoleic Acids; D008042:Linolenic Acids; D010713:Phosphatidylcholines; C009909:monogalactosyldiacylglycerol; D014531:Uridine Diphosphate Galactose