Altered lipolytic response to glucagon and adenosine deaminase in adipocytes from starved rats.
1. Adipocytes isolated from epididymal adipose tissue of fed or 24 h-starved rats were incubated with a range of glucagon concentrations in the presence and absence of adenosine deaminase (4 munits/ml). 2. With adenosine deaminase present, the lipolytic response to low concentrations of glucagon (1-6 ng/ml) was considerably enhanced in cells from starved rats. 3. The effect of adenosine deaminase on basal lipolysis was altered after starvation. 4. D-3-Hydroxybutyrate (5 mM) decreased the sensitivity of lipolysis to glucagon. 5. The possible involvement of glucagon-stimulated lipolysis in the regulation of ketogenesis is briefly discussed.
D000243:Adenosine Deaminase / Q000494:pharmacology*; D000273:Adipose Tissue / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D005934:Glucagon / Q000494:pharmacology*; D006885:Hydroxybutyrates / Q000494:pharmacology; D066298:In Vitro Techniques; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D009700:Nucleoside Deaminases / Q000494:pharmacology*; D051381:Rats; D013217:Starvation / Q000378:metabolism; D013268:Stimulation, Chemical
D006885:Hydroxybutyrates; D005934:Glucagon; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase