[Determination of phospholipase A2 activity in liver mitochondria using fluorescent dye 1,8-anilinonaphthalene sulfonate].
Quantitative estimation of free fatty acids was carried out in liver mitochondria of normal and thyroidectomized rabbits by means of two procedures--spectrophotometry using rhodamine 6Zh and a fluorescent method with 1,8-aniline naphthalene sulfonate /ANS/ as a dye. The use of ANS enabled to estimate quantitatively the free fatty acids beginning from 25 nmol in a sample /with eggor of about 17%/ by visual evaluation of the spot areas. As compared with the rhodamine procedure, use of ANS permitted to increase 5-fold the sensitivity of free fatty acid estimation, to elevate 6-fold the accuracy of the analysis, to overcome the step of fatty acid elution from chromatogramms and to decrease the time required for analysis. The data obtained suggest that the hyperthyroid state in rabbits was accompanied by an increase in the phospholipase A2 activity in liver mitochondria.
D000817:Anilino Naphthalenesulfonates; D000818:Animals; D002845:Chromatography; D005230:Fatty Acids, Nonesterified / Q000032:analysis*; D008722:Methods; D008930:Mitochondria, Liver / Q000032:analysis / Q000201:enzymology*; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D011817:Rabbits; D012235:Rhodamines; D013053:Spectrophotometry
D000817:Anilino Naphthalenesulfonates; D005230:Fatty Acids, Nonesterified; D012235:Rhodamines; D010740:Phospholipases; D010741:Phospholipases A; D054467:Phospholipases A2
Opredelenie aktivnosti fosfolipazy A2 v mitokhondriiakh pecheni s pomoshch'iu fliuorestsentnogo krasitelia 1,8-anilinonaftalin sul'fonata.