[Spectrophotometric method of determining aminoglycoside antibiotics].
Azo-derivatives of chromotropic acid and azo-dyes of the stilbene series were studies as possible reagents for photometric determination of aminoglycoside antibiotics (AB) in biological materials. The complex of Pr3+ with stilbazochrome-P (Sch-P) proved to be the best precipitant of AB. P (Sch-P) with Pr3+ and AB in acetate and citrate buffer-solutions formed a precipitate with AB:Pr:Sch-P = 1 : 1 : 3. Most of the metallic cations and anions did not prevent formation of the precipitate in the citrate buffer. A procedure for determination of 0.01--100 micrograms of AB in biological materials was developed with the use of Sch-P. The procedure is based on separation of the AB:Pr:Sch-P precipitate and photometry of the sypernatant solution at 560 nm.
D000617:Aminoglycosides / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001150:Arsenazo III / Q000031:analogs & derivatives / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D011221:Praseodymium / Q000494:pharmacology; D013053:Spectrophotometry / Q000379:methods*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D001150:Arsenazo III; C027467:stilbazochrome-P; D011221:Praseodymium
Spektrofotometricheskiĭ metod opredeleniia aminoglikozidnykh antibiotikov.