Assessment of somatogenotoxicity of povidone-iodine using two in vitro assays.
The mutagenic potential of povidone-iodine (PVP-I) and some related compounds were studied using the L5178Y mouse (TK+/-) lymphoma assay. The established mutagens ethyl methanesulfonate (EMS) and dimethylnitrosamine (DMN) were highly active in this assay, whereas PVP-I, polyvinyl pyrolidone (PVP), potassium iodide (KI), and iodine (I2) were inactive. Furthermore, in the presence of a rat liver microsomal activating fraction (S-9), PVP-I and I2 had only marginal activity as mutagens. Using the Balb/c 3T3 transformation assay we assessed the transformational capacities of these same agents and the positive mutagen N-methyl-N-nitro-N-nitrosoguanidine (MNNG). All concentrations of the compounds tested were inactive in this assay except for PVP-I (5 mg/ml) and MNNG (5 micrograms/ml). However, the response with PVP-I was only marginal. We concluded from these studies that PVP, PVP-I, KI, and I2 did not possess any biologically significant mutagenic or cell transforming ability.
D000818:Animals; D002273:Carcinogens; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced; D007455:Iodine / Q000633:toxicity; D007940:Leukemia L5178; D051379:Mice; D008807:Mice, Inbred BALB C; D009152:Mutagenicity Tests; D009153:Mutagens; D011193:Potassium Iodide / Q000633:toxicity; D011205:Povidone / Q000031:analogs & derivatives* / Q000633:toxicity; D011206:Povidone-Iodine / Q000633:toxicity*
D002273:Carcinogens; D009153:Mutagens; D011193:Potassium Iodide; D011206:Povidone-Iodine; D007455:Iodine; D011205:Povidone