[Preliminary results on the possible role of intestinal peroxidase (EC 1.11.1.7) in the biosynthesis of prostaglandins].
The ability of a partially purified rat ileum peroxidase to synthetize PGE2 and PGF2 alpha from arachidonic acid was researched. The enzyme preparation, when incubated anaerobically with H2O2 and arachidonic acid, produced a compound extractable with ethyl acetate which showed on TLC the same Rf as authentic PGE2. This compound, after elution from the gel, was able to contract the rat strip fundus preparation.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D001681:Biological Assay; D002855:Chromatography, Thin Layer; D005748:Gastric Fundus / Q000187:drug effects; D006861:Hydrogen Peroxide; D008297:Male; D010544:Peroxidases / Q000378:metabolism*; D011458:Prostaglandins E / Q000096:biosynthesis* / Q000494:pharmacology; D011460:Prostaglandins F / Q000096:biosynthesis*; D051381:Rats
D001095:Arachidonic Acids; D011458:Prostaglandins E; D011460:Prostaglandins F; D006861:Hydrogen Peroxide; D010544:Peroxidases
Risultati preliminari sul possibile ruolo della perossidasi intestinale (EC 1.11.1.7) nella sintesi delle prostaglandine.