title: Comparative biochemistry of murine arylsulfatase B.
abstract: Arylsulfatase B was purified 4500-fold from liver and kidney of C57BL/6J mice. Hepatic and renal arysulfatase B are apparently determined by a single structural locus; however, posttranslational modification introduces inter- and intratissue microheterogeneity. Partially purified enzyme from C57BL/6J, A/J, C3H/HeJ, and SWR/J mice has similar catalytic properties. The 4500-fold-purified arylsulfatase B from SWR/J and C3H/HeJ mice was more thermostable than that from C57BL/6J and A/J mice, strongly suggesting that the thermostability difference reflects an alteration of the primary structure of the enzyme. Thermal stability of arylsulfatase B was pH dependent and markedly influenced by buffer anion. Variation of thermostability did not appear accountable for the observed activity variation among these strains; however, this possibility cannot be rigorously excluded by presently available data. Thirty-five murine strains were found to possess the As-1a allele (thermostable enzyme), while As-1b was largely restricted to A and C57 strains.
mesh_terms: D000818:Animals; D001192:Arylsulfatases / Q000235:genetics / Q000378:metabolism*; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D014644:Genetic Variation; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics / Q000378:metabolism*; D009439:Neuraminidase / Q000378:metabolism; D013045:Species Specificity; D013429:Sulfatases / Q000378:metabolism*
chemical_list: D013429:Sulfatases; D001192:Arylsulfatases; D009439:Neuraminidase