High-performance liquid chromatography and thin-layer chromatography of anthracycline antibiotics: separation and identification of components of the dauno-rubicin complex from fermentation broth.
A new solvent system composed of methanol-acidic water (pH 2.0 with phosphoric acid) has been developed for high-performance liquid chromatography of the daunorubicin complex from fermentation broth on a micro Bondapak C18 column. Application of this solvent system in conjunction with thin-layer chromatography in the identification of anthracycline antibiotics from the fermentation broth is discussed.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D003630:Daunorubicin / Q000302:isolation & purification*; D000432:Methanol; D010756:Phosphoric Acids
D010756:Phosphoric Acids; D000432:Methanol; D003630:Daunorubicin