[Colorimetric determination of cholesterol in biological fluids using 2,3-naphthalenedicarboxaldehyde (author's transl)].
The authors describe a new method of cholesterol determination, using the intense blue color given by interaction with 2,3-naphthalenedicarboxaldehyde in a sulphoacetic medium. The degree of precision of the method, as well as its reproducibility are discussed; a good correlation of the new method with two classical techniques, the colorimetric method of Liebermann and the enzymatic method, is observed. The high sensitivity and the specificity of the colour reaction allow the choice of the method for a direct determination of serum cholesterol using a 10 microliter sample.
D002784:Cholesterol / Q000032:analysis* / Q000097:blood; D003124:Colorimetry / Q000379:methods; D007202:Indicators and Reagents; D009281:Naphthalenes; D013261:Sterols / Q000032:analysis
D007202:Indicators and Reagents; D009281:Naphthalenes; D013261:Sterols; C027717:2,3-naphthalenedicarboxaldehyde; D002784:Cholesterol
Dosage colorimétrique du cholestérol dans les milieux biologiques par le naphtalène dicarboxaldéhyde-2,3.