Identification of H-2-controlled structural variants of the murine Slp protein and demonstration of cis-regulation of its expression.
H-2 haplotype-related structural variation in Slp beta-chains was detected by the limited proteolysis peptide mapping technique. Two distinct peptide patterns were distinguished: Slp.1 was found in strains carrying the S regions of the H-2d and H-2s haplotypes, and Slp.2 was found in strains bearing the S region of H-2w7. These different patterns were expressed codominantly in male (Sd X Sw7)F1 hybrids, whereas only the Slp.2 pattern was expressed in female heterozygotes. The 2 beta-chains are most likely the products of alleles of the Slp structural gene, which must then be located in the murine major histocompatibility complex, very probably in the S region.
D000818:Animals; D005260:Female; D014644:Genetic Variation; D006183:H-2 Antigens / Q000235:genetics*; D006238:Haploidy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D010455:Peptides
D006183:H-2 Antigens; D010455:Peptides