[Double-helical structures in the make up of poly(A)-containing RNA of rat liver cells. Effect of cortisone].
Cytoplasmic poly(A)-containing RNA from rat liver cells are shown to contain RNase-resistant double-stranded regions. Their content increases after cortisone injection. The isolated double-stranded (ds) regions in polyacrylamide gel electrophoresis are seen as discrete bands of three size classes. Their melting curves are typical for double-stranded nucleic acids. The ds-structure isolated from poly(A)+-RNA of cortisone-stimulated rat livers has higher thermostability, with that of control livers. After denaturation 90% of ds-regions become single-stranded. About 50% of denatured double-stranded fragments reassociate at the range of COT = 10(-3)-10(-2). The origin of ds-structures in cytoplasmic poly(A)+-RNA and their possible functions are discussed.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003348:Cortisone / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D008099:Liver / Q000032:analysis*; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D011061:Poly A; D012330:RNA, Double-Stranded / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D012260:Ribonucleases; D013268:Stimulation, Chemical; D014158:Transcription, Genetic / Q000187:drug effects*
D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D011061:Poly A; D012260:Ribonucleases; D003348:Cortisone
Dvuspiral'nye struktury v sostave poli(A)-soderzhashchikh RNK tsi toplazmy kletok pecheni krys. Vliianie kortizona.