Alkylation of rat serum proteins by dimethylnitrosamine and acetylaminofluorene.
The labelling of rat serum proteins was studied after i.p. injections of radioactive dimethylnitrosamine (DMN) or acetylaminofluorene (AAF). Protein-bound radioactivity decayed with an apparent half-life of 49 h and 35 h after DMN and AAF treatment, respectively. Methylated amino acid residues were indentified from DMN-treated animals using thin-layer chromatography of the serum protein hydrolysates. The main methylation products were shown to be S-methylcysteine and 1-methylhistidine. The possibilities of monitoring carcinogen and mutagen exposure by analysing the alkylation of serum proteins are discussed.
D015073:2-Acetylaminofluorene / Q000378:metabolism*; D000478:Alkylation; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D004128:Dimethylnitrosamine / Q000378:metabolism*; D051381:Rats
D001798:Blood Proteins; D015073:2-Acetylaminofluorene; D004128:Dimethylnitrosamine