title: Effects of selective alkylphosphorylation of propionylcholinesterase on the regeneration of acetylcholinesterase in the aqueous soluble fraction of superior cervical ganglia of the rat following sarin.
abstract: The rates of regeneration of acetylcholinesterase (AChE) and propionylcholinesterase (PrChE) in the supernatants of aqueous homogenates of rat superior cervical ganglia, centrifuged at 100,000 g for 90 min, were determined at 1, 3, 6, and 16 h following their inactivation (greater than 90%) by administration of sarin, 2.0 mumol/kg i.v. Values were compared with those in animals in which the PrChE was continually suppressed by the repeated, fractional administration of iso-OMPA, in a total dose of 10 or 20 mumol/kg i.p. These doses of iso-OMPA alone produced 96-99% inactivation of PrChE with no detectable effect on AChE. Significant suppression of AChE regeneration by iso-OMPA administration was noted only at 6 h; in contrast with earlier findings in the cat, administration of iso-OMPA alone caused no significant increase in ganglionic AChE activity.
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002802:Cholinesterases / Q000378:metabolism*; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010766:Phosphorylation; D051381:Rats; D012524:Sarin / Q000494:pharmacology*; D012995:Solubility; D013347:Subcellular Fractions / Q000201:enzymology; D013770:Tetraisopropylpyrophosphamide / Q000494:pharmacology
chemical_list: D009943:Organophosphorus Compounds; D013770:Tetraisopropylpyrophosphamide; D012524:Sarin; C025181:propionylcholinesterase; D000110:Acetylcholinesterase; D002802:Cholinesterases