[Determination of morphine and codeine in urine by high-pressure liquid chromatography (author's transl)].
A sensitive and specific method for the determination of morphine and codeine is described. The drugs are adsorbed from urine on Amberlite XAD-2 resin at pH 8.5 and eluted from it with organic solvent. After a clean up the residue is separated by high-pressure-liquid-chromatography on reversed phase column using 0.1 M NaH2PO4 in 25% CH3CN/H2O as mobile phase. The eluted drugs are detected by uv-absorption at 230 nm. The method is sensitive, specific and precise.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003061:Codeine / Q000652:urine*; D006801:Humans; D009020:Morphine / Q000652:urine*
D009020:Morphine; D003061:Codeine
Bestimmung von Morphin und Codein im Urin mit Hilfe der Hochdruckflüssigchromatographie.