Large-scale purification of prothoracicotropic hormone of the silkworm (Bombyx mori).
Prothoracicotropic hormone has been successfully extracted from as many as 2,800,000 silkworm male adult heads and purified partially through an 8-step procedure. Approximately 7 microgram of the crude preparation ("crude PTTH") shows activity in the debrained Samia pupa test. A further 4-step chromatographic purification of the "crude PTTH" derived from 100,000 Bombyx heads yields 3.4 mg of purified preparation ("highly purified PTTH"), of which 7 ng causes adult development in a debrained Samia pupa.
D000818:Animals; D012831:Bombyx / Q000032:analysis*; D007301:Insect Hormones / Q000302:isolation & purification* / Q000494:pharmacology; D008297:Male; D008722:Methods; D009024:Morphogenesis / Q000187:drug effects; D011679:Pupa / Q000502:physiology
D007301:Insect Hormones