In vitro effects of saccharin on cell-mediated host defence mechanisms.
In vitro exposure to saccharin (0.1-2 mg/ml) did not affect the tumoricidal activity of macrophages and natural killer (NK) cells from mice and rats. In contrast saccharin at doses of 0.5-2 mg/ml significantly and consistently depressed the blastogenic response of rat lymph node cells to phytohemagglutinin (PHA). Similarly mouse splenocytes showed impaired responsiveness to PHA and bacterial lipopolysaccharides n the presence of 2 mg/ml saccharin. Compared with murine lymphoid cells, human peripheral blood lymphocytes were relatively resistant to the immunodepressive activity of saccharin, a low but significant, depression (21%) of the response to PHA being observed only at the highest concentration (2 mg/ml) in 1 of 3 normal healthy donors tested.
D000818:Animals; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D010835:Phytohemagglutinins / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D012439:Saccharin / Q000276:immunology*
D010835:Phytohemagglutinins; D012439:Saccharin