title: Characterization of multiple forms of acetylcholinesterase in electric organ of Torpedo marmorata.
abstract: Three molecular forms of acetylcholinesterase can be isolated by velocity sedimentation from extracts of electric organ from Torpedo marmorata. Their apparent sedimentation coefficients are 6 S, 13 S and 17 S. Characteristic differences are found with regard to their solubilization properties. The 13 S and 17 S forms are extracted only in the presence of high salt concentrations. At low ionic conditions only a fraction of the 6 S form is solubilized. Quantitative recovery of this form can be achieved in the presence of Triton X-100 at concentrations of 0.5-1%. The relative ratio of the various forms changes during the development of the fish.
mesh_terms: D000110:Acetylcholinesterase / Q000302:isolation & purification*; D000367:Age Factors; D000818:Animals; D004557:Electric Organ / Q000201:enzymology*; D005399:Fishes / Q000378:metabolism*; D007527:Isoenzymes / Q000302:isolation & purification*; D008722:Methods; D008970:Molecular Weight; D012995:Solubility
chemical_list: D007527:Isoenzymes; D000110:Acetylcholinesterase