Different patterns of replication in influenza virus-infected KB cells.
When KB cells were infected either with the fowl plague (FPV) Rostock strain (Hav1N1) or the WSN (H0N1) strain of influenza A virus the yield of cell-associated haemagglutinin and neuraminidase polypeptides was essentially comparable, but virus particles were not produced in the FPV-KB system. WSN virus-infected KB cells synthesized normal amounts of mature virus particles and had all the characteristics of a permissive replication cycle. Biosynthesis and transport of RNP antigen from nucleus to cytoplasm of infected cells were traced by immunofluorescent staining at 4 and 8 hours after the beginning of infection. While the fluorescent-stained material was totally confined to the nuclei in FPV-infected KB cells, RNP antigen migrated out of the nucleus during the replicative cycle of WSN virus in the same host cell. Patterns of virus-specific protein synthesis were studied by pulse-labelling with 35S-methionine. The most significant feature concerned the amplification of synthesis of virus-induced matrix (M) protein which did not occur in FPV-infected cells but occurred normally during WSN infection. The different patterns of replication in the same host cell when infected by different influenza A viruses is discussed.
D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006389:Hemagglutinins, Viral / Q000032:analysis; D006801:Humans; D009980:Influenza A virus / Q000254:growth & development* / Q000378:metabolism; D009439:Neuraminidase / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D006389:Hemagglutinins, Viral; D012261:Ribonucleoproteins; D014764:Viral Proteins; D009439:Neuraminidase