Extraction and quantification of daunomycin and doxorubicin in tissues.
The measurement of intracellular concentrations of the anti-cancer drug doxorubicin was performed by the application of a simple cell extraction technique combined with a rapid high-performance liquid chromatographic separation. Quantitation was done by fluorescence detection. The extraction procedure was non-degradative and the mean recovery of drug was 95%. A high drug extraction efficiency was confirmed with radiolabeled [3H] doxorubicin. The method is applicable to normal and neoplastic tissue.
D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003630:Daunorubicin / Q000032:analysis* / Q000494:pharmacology; D004317:Doxorubicin / Q000032:analysis* / Q000494:pharmacology; D006801:Humans; D007938:Leukemia / Q000097:blood; D008214:Lymphocytes / Q000032:analysis; D009000:Monocytes / Q000032:analysis
D004317:Doxorubicin; D003630:Daunorubicin