title: Transcapillary exchange and distribution of carbon-11 labelled ethanol in the isolated perfused rat liver.
abstract: The distribution kinetics of ethanol in the isolated perfused rat liver at various flow rates and ethanol concentrations were studied by the indicator diffusion technique using 11C-labelled ethanol. A double tracer solution with 99mTc-labelled red blood cells as the vascular reference and 11C-ethanol as the test substance was injected into the catheter connected to the portal vein. Time-activity curves were monitored by a NaI(Tl) scintillation detector above the outflow catheter. The following values were obtained: 0.95 +/- 0.01 for the maximal extraction (Emax), 3.0 +/- 0.1 x flow (r = 0.98) for the capillary permeability surface area product (PScap), 18.7 +/- 2.5% for the vascular volume (V1) and 56.2 +/- 9.0% for the ethanol distribution volume (V2). The volumes were calculated from the products of mean transit time (MTT) and flow (f), and PScap was calculated from the equation PScap = -f x ln(1-Emax). The results show that the ethanol distribution in the liver is flow limited since Emax was constant at various flow rates (0.67-2.55 ml/g x min-1). The distribution volumes obtained agree well with the values for liver vascular volume and water distribution volume reported in the literature.
mesh_terms: D000818:Animals; D002199:Capillary Permeability; D002250:Carbon Radioisotopes; D000431:Ethanol / Q000097:blood*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008102:Liver Circulation; D008297:Male; D010477:Perfusion; D051381:Rats
chemical_list: D002250:Carbon Radioisotopes; D000431:Ethanol