A nitrogen pressure-shearing apparatus for preparing distinct populations of chromatin fractions.
A method for preparing reproducible chromatin fractions is described. Fragmentation is accomplished by expelling whole chromatin from an enclosed stainless-steel chamber through a restricted orifice utilizing low nitrogen pressure. Sheared chromatin is fractionated into two distinct heavy and light fractions by centrifugation on 5-20% linear sucrose gradient.
D002458:Cell Fractionation / Q000295:instrumentation*; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000302:isolation & purification*
D002843:Chromatin