The association of alpha-actinin with the plasma membrane.
The role of alpha-actinin in the attachment of actin to plasma membranes has been investigated. Specific antibody staining of SDS gels has indicated that alpha-actinin is a major component in isolated plasma membranes prepared from three different cell types by two different procedures. Using specific extraction conditions, most of the alpha-actinin can be selectively extracted from the membranes with relatively little parallel release of actin. This selective dissociation of alpha-actinin from the plasma membrane leads us to conclude that alpha-actinin is present in these membrane preparations, because it is bound to actin, and that alpha-actinin does not form a direct link between actin and the membrane.
D000185:Actinin / Q000378:metabolism*; D000818:Animals; D000906:Antibodies; D001665:Binding Sites; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D009124:Muscle Proteins / Q000378:metabolism*; D010954:Plasmacytoma; D011485:Protein Binding
D000906:Antibodies; D009124:Muscle Proteins; D000185:Actinin