[Isolation and characteristics of the 8--10S poly(A)-containing mRNA fraction from mid-blastula stage Stronglyocentrotus intermedius sea urchin embryos].
Centrifugation in sucrose linear gradient and affinity chromatography on oligo (dT)-cellulose were used to isolate the poly(A)-containing fraction of 8--10S RNA from St. intermedius sea urchin embryos at the middle blastula stage. This RNA has been shown to possess template activity in the cell-free protein-synthesizing system from wheat germs. The studied mRNA fraction induces synthesis of two polypeptides with the molecular weights of 10 and 15 thousand daltons according to the data of electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulphate. The products of in vitro translation were immunochemically identified with embryo-specific glycoproteins of the plasma membrane of embryo cells.
D000818:Animals; D001755:Blastocyst / Q000032:analysis*; D002462:Cell Membrane / Q000378:metabolism; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D011061:Poly A; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism; D012617:Sea Urchins / Q000032:analysis* / Q000235:genetics
D006023:Glycoproteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D011061:Poly A
Vydelenie i kharakteristika fraktsii 8--10S poli(A)-soderzhashchikh mRNK iz émbrionov morskogo ezha Strongylocentrotus intermedius na stadii sredneĭ blastuly.