Studies on the assembly of the rat lens capsule. Biosynthesis of a cross-linked collagenous component of high molecular weight.
1. Intact rat lenses in tissue culture synthesize hydroxy[3H]proline-containing polypeptides of apparent mol.wt. approx. 180000, which become assembled into aggregates of higher molecular weight with time. 2. Both the 180000-mol.wt. species and the aggregates are components of the deoxycholate-insoluble base-membrane matrix. 3. Formation of the high-molecular-weight aggregate is accompanied by the biosynthesis of the reducible hydroxylysine-derived cross-link hydroxylysino-5-oxo-norleucine. 4. Hydroxylysino-5-oxonorleucine and dehydrohydroxylysinonorleucine are the major reducible cross-links present in intact foetal and 1-month-old calf lens capsules.
D000818:Animals; D001894:Borohydrides / Q000494:pharmacology; D002850:Chromatography, Gel; D003094:Collagen / Q000096:biosynthesis*; D046508:Culture Techniques; D004151:Dipeptides / Q000096:biosynthesis; D007908:Lens, Crystalline / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D010084:Oxidation-Reduction; D051381:Rats
D001894:Borohydrides; D004151:Dipeptides; C028366:hydroxylysyl-5-oxonorleucine; D003094:Collagen