Improved recovery of somatomedin activity from plasma by prolonged formic acid extraction.
Recovery of somatomedin activity from plasma fractions was increased approximately 5-fold by prolonged extraction of isolates with formic acid. Extraction for 72 hours enhanced the yields of active peptides in the 6,000, 8,000 and > 20,000 dalton range. Yields of smaller peptides were similar following short (2 hrs) and long (72 hrs) extraction periods. Active fractions obtained after prolonged extraction possessed sulfation activity and insulin-receptor affinity comparable to somatomedin isolated during short extraction with formic acid.
D000818:Animals; D002356:Cartilage / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D005561:Formates; D007016:Hypophysectomy; D051381:Rats; D011972:Receptor, Insulin / Q000378:metabolism; D013002:Somatomedins / Q000097:blood / Q000302:isolation & purification*; D013455:Sulfur / Q000378:metabolism; D013997:Time Factors
D005561:Formates; D013002:Somatomedins; D013455:Sulfur; D011972:Receptor, Insulin