title: Hexose transport and phosphorylation by Novikoff rat hepatoma cells as function of extracellular pH.
abstract: The effect of extracellular pH on hexose uptake by cultured Novikoff hepatoma cells has been examined with respect to four distinct parameters: i) the transport of 3-O-methyl-D-glucose, ii) the uptake of 2-deoxy-D-glucose by intact cells, iii) the phosphorylation of 2-deoxy-D-glucose in vitro by cell-free preparations, and iv) the intracellular pH as measured by 5,5'-dimethyl[2-14C]oxazolidine-2,4-dione. Transport per se was not affected by pH in the range of 6 to 8, while uptake of 2-deoxy-D-glucose increased with pH 2- to 3-fold over this range. The pH sensitivity of uptake can be explained on the basis of a change in hexokinase activity effective in situ. The pH dependence of hexokinase observed in vitro, however, was insufficient to account for that apparent in situ.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D010766:Phosphorylation; D051381:Rats
chemical_list: D003837:Deoxy Sugars; D008757:Methylglucosides; D008759:Methylglycosides; D003847:Deoxyglucose; D006593:Hexokinase