title: Microwave irradiation and in vitro release of enzymes from hepatic lysosomes.
abstract: An exposure system for examining in vitro effects of microwave irradiation on cellular and subcellular components has been developed. The system was used to test the effect of 2.45-GHz CW microwaves on the release of two lysosomal enzymes. At a specific absorption rat (SAR) of 10, 50 or 100 mW/g (90 min) no effects were noted at 37 degrees C (pH of 7.3) on lysosomal fragility as determined by the release of the lysosomal enzymes cathepsin D and beta-glucuronidase. When the medium was adjusted to pH 5.0, microwave irradiation of the lysosomal suspension had no effect on the acid-induced enhancement of release of lysosomal enzymes. The data indicate that microwave irradiation had no labilizing effect on the lysosomal membrane, although other microwave-membrane interactions not associated with enzyme release may occur.
mesh_terms: D000818:Animals; D002402:Cathepsin D; D002403:Cathepsins / Q000032:analysis; D005260:Female; D005966:Glucuronidase / Q000032:analysis; D006168:Guinea Pigs; D008099:Liver / Q000201:enzymology / Q000528:radiation effects* / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology / Q000528:radiation effects*; D008872:Microwaves / Q000009:adverse effects*
chemical_list: D005966:Glucuronidase; D002403:Cathepsins; D002402:Cathepsin D