Storage and release of noradrenaline in hypothalamic synaptosomes.
The noradrenaline storage capacity of vesicles in hypothalamic synaptosomes was measured by incubating them with [3H]noradrenaline under saturating conditions. The normal noradrenaline content is 52% of storage capacity. Incubation or superfusion with 50 mM-potassium causes calsium-dependent release from the vesicles. Such release reduces not only the vesicular content, but also the noradrenaline storage capacity. This suggests that after exocytosis vesicles cannot refill with noradrenaline.
D000818:Animals; D001692:Biological Transport; D007031:Hypothalamus / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D013572:Synaptic Vesicles / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*
D009638:Norepinephrine