Polyacrylamide gel electrophoresis of proteins from human skin scalded in vitro and dissolved with dodecylsulphate.
Human skin was scalded in vitro at different temperatures. Strips of scalded skin were dissolved in sodium dodecylsulphate and submitted to polyacrylamide gel electrophoresis. Negative correlation was found between temperature of scalding and relative heights of some of the peaks estimated by densitography. Human skin scalded at temperature up to 100 degrees C did not show qualitative changes in protein composition. Appearance of high molecular weight protein fractions in skin scalded at 120 degrees C was demonstrated.
D002056:Burns / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006023:Glycoproteins / Q000032:analysis* / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D008074:Lipoproteins / Q000032:analysis* / Q000378:metabolism; D011489:Protein Denaturation; D012867:Skin / Q000032:analysis* / Q000293:injuries; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D012995:Solubility
D006023:Glycoproteins; D008074:Lipoproteins; D012967:Sodium Dodecyl Sulfate