In vitro development of CFU-E and BFU-E in cultures of embryonic and post-embryonic chicken hematopoietic cells.
A culture method is proposed for the in vitro development of chicken erythrocytic progenitors. When grown with avian erythropoietin, Colony Forming Unit Erythrocytic (CFU-E) and Burst Forming Unit-Erythrocytic (BFU-E) give rise respectively to erythrocytic colonies and bursts within 3 and 6 days. BFU-E development is greatly enhanced by pokeweed-mitogen-spleen-cell conditioned medium and requires higher erythropoietin concentrations than for CFU-E. An antigen specific to immature red cells can be detected on CFU-E but not on BFU-E, showing that both progenitors represent distinct entities. BFU-E and CFU-E are found in embryonic marrow and yolk sac. In the young blastoderm BFU-E becomes detectable at the primitive streak stage.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001854:Bone Marrow Cells; D002455:Cell Division; D002478:Cells, Cultured; D002642:Chick Embryo; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004920:Erythropoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D015017:Yolk Sac / Q000166:cytology
D000954:Antigens, Surface; D003470:Culture Media