Solid-phase synthesis of polynucleotides. IV. Usage of polystyrene resins for the synthesis of polydeoxyribonucleotides by the phosphostriester method.
Contrary to the expectation, the Merrifield polystyrene resin, 2% cross-linked by divinylbenzene, is as efficient as the polyacrylmorpholide resin for the synthesis of polydeoxyribonucleotides using a phosphotriester method. On the Merrifield resin, the tetradecamer, dTpCpGpTpCpApApCpTpGpGpCpTpT, and the hexadecamer, dCpCpApGpTpCpApCpGpApCpGpTpTpGpT, were synthesized by the phosphotriester method using di and trinucleotide blocks as coupling units.
D001483:Base Sequence; D007202:Indicators and Reagents; D008722:Methods; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis*; D011131:Polyribonucleotides / Q000138:chemical synthesis*; D011137:Polystyrenes; D012116:Resins, Plant
D007202:Indicators and Reagents; D011089:Polydeoxyribonucleotides; D011131:Polyribonucleotides; D011137:Polystyrenes; D012116:Resins, Plant