A new procedure for analysing the relationship between different cell surface antigens.
An immunoprecipitation-inhibition procedure is assessing whether different antigenic determinants are carried on the same molecule or on different molecules on cells. The procedure entails (a) exposing NP-40 lysates of cells to antibody against one antigenic specificity; (b) removing free antibody and immune complexes by absorption with protein A-bearing S. aureus bacteria; (c) adsorption of the NP-40 with a detergent binding resin; and (d) measuring the inhibitory activity of the lysates for antibody against another specificity by a rosetting assay. This method has several advantages over the widely used sequential immunoprecipitation procedure.
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D011232:Chemical Precipitation; D003902:Detergents / Q000494:pharmacology; D005260:Female; D007519:Isoantigens / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology
D000954:Antigens, Surface; D003902:Detergents; D007519:Isoantigens