Trisomic analysis of isozymic loci in tomato species: segregation and dosage effects.
Five isozymic loci were localized in the tomato (Lycopersicon esculentum) genome by trisomic analysis. Results revealed the following locations: Aps-1 on chromosome 6, Est-1 and Prx-2 on chromosome 2, Prx-4 on chromosome 10, and Prx-7 on chromosome 3. Three genes--Aps-1, Prx-2, and Prx-4--showed an arithmetic increase in allozyme concentration in direct proportion to the increase of gene dosage in respective primary trisomics. In contrast, no increase in relative Est-1 isozyme concentration was observed for any primary trisomic type. The phenotypes of the Aps-1, Prx-2, and Est-1 genes showed a pattern of banding intensity proportional to the allelic ratio (+/+/a vs. +/a/a) in primary trisomics; zymotypes of these differential trisomic heterozygotes appeared as "converse images" of each other.
D000135:Acid Phosphatase / Q000235:genetics; D000483:Alleles; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004586:Electrophoresis; D004950:Esterases / Q000235:genetics; D008040:Genetic Linkage; D007527:Isoenzymes / Q000235:genetics*; D010544:Peroxidases / Q000235:genetics; D010944:Plants / Q000201:enzymology / Q000235:genetics*; D014314:Trisomy
D007527:Isoenzymes; D010544:Peroxidases; D004950:Esterases; D000135:Acid Phosphatase