Enhancement of urinary prostaglandin excretion by chlorazanil in rats. Effects of indomethacin.
In conscious unloaded Sprague-Dawley female rats chlorazanil (10 mg/kg orally) markedly increased urinary prostaglandin E2-excretion from 51 +/- 9 to 813 +/- 112 ng/kg/6 hrs. Urinary flow rate increased from 12.8 +/- 0.6 to 42.0 +/- 1.4 ml/kg/6 hrs and urinary sodium excretion from 0.96 +/- 0.12 to 3.86 +/- 0.33 mmol/kg/6 hrs Urinary potassium excretion was unchanged. Indomethacin pretreatment (5 mg/kg orally) greatly induced the effect of chlorazanil on urinary prostaglandin E2-excretion. In addition indomethacin modified the excretory effects of chlorazanil, thus the enhancement of urinary flow rate was attenuated, the potassium excretion decreased, while sodium excretion tended to be potentiated. In non-pretreated condition chlorazanil variably affected urinary kallikrein excretion (TAMe-esterase activity) from 108 +/- 6 to 92 +/- 33 mEU/kg/6 hrs. After indomethacin pretreatment chlorazanil invariably reduced urine enzyme excretion from 111 +/- 6 to 32 +/- 4 mEU/kg/6 hrs.
D000818:Animals; D002722:Chlorobenzenes; D004232:Diuretics / Q000494:pharmacology*; D005260:Female; D007213:Indomethacin / Q000494:pharmacology*; D011453:Prostaglandins / Q000652:urine*; D051381:Rats; D013268:Stimulation, Chemical; D014227:Triazines / Q000494:pharmacology*
D002722:Chlorobenzenes; D004232:Diuretics; D011453:Prostaglandins; D014227:Triazines; C017837:chlorazanil; D007213:Indomethacin