Purification and characterization of cytoplasmic 14C-arginine rich basic proteins of Ehrlich ascites tumor cells.
Arginine rich basic proteins from cytoplasm of Ehrlich ascites tumor cells have been separated and partially characterized. All proteins show a cationic character with the following isoelectric points: 8.45, 8.60, 8.70, 8.90, and possess various amount of arginine. The protein of the highest molecular weight (75000) has the greatest amount of arginine (18.1%), specific radioactivity (19760 cpm/mg, min) and isoelectric point (8.9). The significance of those proteins in the cytoplasm of tumor cells has been discussed briefly.
D000818:Animals; D001120:Arginine / Q000032:analysis; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000032:analysis; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000302:isolation & purification*
D009363:Neoplasm Proteins; D001120:Arginine