title: Excitation-contraction coupling in mammalian cardiac muscle during Ba2+-induced contracture.
abstract: The membrane potential and tension were recorded during contracture of mammalian ventricular muscles bathed in Tyrode solution with Ba2+ in the place of Ca2+. Strength of contracture increased with increasing Ba2+ concentration from 0.1 to 4 mM and reached a plateau above 4 mM. The membrane potential was depolarized to about -35 mV with 0.1 mM Ba2+ and to -20 mV with 5 mM Ba2+. When 0.5 mM Mn2+ was added to Ba2+-Tyrode solution, in which the muscle had been in a state of steady contracture, the muscle immediately started to relax to the resting level. In contrast, addition of 2 micrograms/ml epinephrine to Ba2+-Tyrode solution increased the strength of the contracture. Neither epinephrine nor Mn2+ caused any noticeable changes of the depolarized membrane potential. The results suggest that Ba2+ not only activates the contractile system but also depolarizes the membrane potential independently of activating the contractile system. These two remarkable actions of Ba2+ seem to be responsible for the long-lasting contracture.
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001464:Barium / Q000494:pharmacology*; D002415:Cats; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology; D066298:In Vitro Techniques; D008345:Manganese / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects*; D009200:Myocardial Contraction; D010210:Papillary Muscles / Q000502:physiology*; D011817:Rabbits
chemical_list: D001464:Barium; D008345:Manganese; D004837:Epinephrine