Terminal deoxynucleotidyl transferase expression in a Thy-l alloantigen variant lymphoma cell line.
Terminal deoxynucleotidyl transferase (TdT) expression was examined in the clones of the radiation induced murine leukemia, RL male 1, which differ in Thy-1.2 alloantigen expression and tumourigenicity in syngeneic mice. Both cell lines displayed predominant cytoplasmic localization of TdT and equal sensitivities to specific TdT inhibitors. The R1 male 1.3 + cell line (Thy-1.2 positive and tumourigenic) demonstrated overall higher levels of TdT activity and different elution patterns on phosphocellulose chromatography compared with the RL male 1.4 - (Thy-1.2 negative and poorly tumourgenic) cell line. These findings suggest an association of TdT expression with tumourigenicity properties in leukemic T lymphocytes.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D003593:Cytoplasm / Q000201:enzymology; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006160:Guanosine Triphosphate / Q000378:metabolism; D007519:Isoantigens; D007942:Leukemia, Experimental / Q000201:enzymology* / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D013950:Thymus Gland / Q000276:immunology
D004273:DNA, Neoplasm; D007519:Isoantigens; D006160:Guanosine Triphosphate; D004254:DNA Nucleotidyltransferases; D004253:DNA Nucleotidylexotransferase