A visual assay to monitor purification of cell surface antigens reacting with monoclonal antibodies.
We have developed a visual microtiter assay to detect solubilized cell-surface antigens which react with monoclonal antibodies. The assay depends on the ability of adsorbed monoclonal antibody to bind target cells to microtiter V wells, and the inhibition of binding by antigen. We have used this assay to follow a 600-fold purification of the human lymphocyte differentiation antigen 3A1 extracted from HSB-2 cells. Antigen 3A1 is a glycoprotein with a molecular weight of approximately 40,000 daltons.
D000327:Adsorption; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000302:isolation & purification*; D001666:Binding Sites, Antibody; D002999:Clone Cells / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel
D000906:Antibodies; D000954:Antigens, Surface