Effect of cyclophosphamide on mouse bone marrow and leukemic stem cells.
The colony forming unit assay was introduced in our laboratory for studies in vivo on the differential effect of new antitumor agents on normal bone marrow and tumor cells, the normal (NCFU) and leukemic (LCFU). To verify the technique the effect of Cyclophosphamide (CY) on the total number and the number of stem cells in bone marrow was examined. Different sensitivity of the mouse normal as compared with leukemic stem cells (leukemia P-388) was shown.
D000818:Animals; D001853:Bone Marrow / Q000473:pathology*; D002452:Cell Count; D003114:Colony-Forming Units Assay; D003520:Cyclophosphamide / Q000627:therapeutic use*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D007941:Leukemia P388 / Q000188:drug therapy / Q000473:pathology; D007945:Leukemia, Lymphoid / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D051379:Mice
D003520:Cyclophosphamide