Poor performance of the isolated rabbit kidney perfused with free-cell media and whole blood.
All of these results indicate that the isolated rabbit kidney, perfused either with free-cell media or with whole blood, shows marked functional deficiencies and cannot be considered a reliable preparation. The former media do not induce vasoconstriction but are unable to insure a correct cations reabsorption, while the latter causes intense renal vasospasm and clogging of the glomerular circulation indicated by high arterial pressure and hematuria. On the basis of these observations it appears indispensable to use a perfusion medium capable of eliciting renal autoregulation but deprived of blood elements such as platelets and leukocytes that may be either directly or indirectly responsible for the glomerular clogging. This problem is considered in the following communication.
D000818:Animals; D001769:Blood; D002474:Cell-Free System; D007668:Kidney / Q000502:physiology*; D010477:Perfusion / Q000379:methods*; D011817:Rabbits; D013347:Subcellular Fractions