A rapid chromatographic procedure for the isolation and quantitation of 1-(2-Aminoethyl)-3-(2,6-dichlorophenyl) thiourea from dietary admix followed by HPLC.
An analytical procedure is described for the determination of 1-(2-aminoethyl)-3-(2,6-dichlorophenyl) thiourea in dietary admix. Because of the complex nature of the carrier matrix, sample cleanup was required prior to HPLC separation. Sample cleanup was achieved using a cation-exchange microcolumn which yield highly reproducible results. HPLC separation was accomplished using a 10 mu C18 column, monitoring the column effluent in the ultraviolet region at 254 nm. After sample cleanup, 1-(2-aminoethyl)-3-(2,6-dichlorophenyl) thiourea could be detected at levels as low as 3 ppm in dietary admix.
D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000959:Antihypertensive Agents / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D010670:Phenylthiourea / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D051381:Rats
D000959:Antihypertensive Agents; C028046:1-(2-aminoethyl)-3-(2,6-dichlorophenyl)thiourea; D010670:Phenylthiourea