Different handling of parathyrin by basal-lateral and brush-border membranes of the bovine kidney cortex.
The two parts of the bovine kidney cortex plasma membrane, the basal-lateral and the brush-border membrane, were simultaneously prepared from the same organ. Both types of membrane bound parathyrin, but only from the basal-lateral fraction was the hormone displaceable by its bioactive N-terminal fragment. In parallel, parathyrin-stimulated adenylate cyclase was predominantly found in basal-lateral membranes. The hormone was fragmented by both membrane types. Basal-lateral membranes generated fragments with a rather uniform size distribution (somewhat smaller than the intact peptide) and apparently preferred the hormone itself as a substrate. In contrast, the fragments produced by brush-border membranes were numberous small peptides.
D000262:Adenylyl Cyclases / Q000096:biosynthesis; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D008871:Microvilli / Q000378:metabolism; D010281:Parathyroid Hormone / Q000378:metabolism* / Q000494:pharmacology; D010446:Peptide Fragments / Q000378:metabolism
D010281:Parathyroid Hormone; D010446:Peptide Fragments; D000262:Adenylyl Cyclases