Oxidation of methionine residues in bovine growth hormone by chloramine-T.
The methionine residues in bovine growth hormone were chemically modified wih chloramine-T at pH 7.4 using different chloramine-T methionine molar ratios. Methionine 4 was shown to be the most reactive followed in decreasing order by methionines 148, 123 and 178. With a 50-fold molar excess of chloramine-T over methionine almost full oxidation of all the methionine residues was achieved. The oxidation of the four residues of methionine does not change the growth promoting activity of the hormone.
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002700:Chloramines; D002942:Circular Dichroism; D013006:Growth Hormone; D007202:Indicators and Reagents; D008715:Methionine; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation; D014105:Tosyl Compounds; D014357:Trypsin
D002700:Chloramines; D007202:Indicators and Reagents; D010446:Peptide Fragments; D014105:Tosyl Compounds; C016300:chloramine-T; D013006:Growth Hormone; D008715:Methionine; D014357:Trypsin