Bradykinin depolarises the rat isolated superior cervical ganglion via B2 receptor activation.
Experiments were undertaken to characterise the action of kinins on sympathetic neurones of the rat superior cervical ganglion (SCG) by use of in vitro grease-gap, extracellular recording techniques in conjunction with selective agonists and antagonists for B1 and B2 bradykinin (BK) receptors. Superfusion of BK (10 nM to 10 microM) to the ganglion produced a concentration-related depolarisation (pD2 = 7.02 +/- 0.04, n = 7) which was inhibited by the selective B2 antagonist HOE 140 (10-100 nM), but not by the B1 antagonist Leu8desArg9 BK (1 microM), indomethacin (7 microM) or the nitric oxide synthase inhibitor L-NG-nitroarginine methyl ester (300 microM). DesArg9BK (10 nM to 10 microM) had no effect on membrane potential. Pre-treatment of animals with intravenous bacterial lipopolysaccharide (LPS, 3 mg kg-1) failed to induce B1 receptor-mediated depolarisations of SCG neurones, or change responses to BK (P > 0.05, n = 4). These experiments highlight and characterise the action of BK as a neuromodulator of sympathetic neurones via B2 receptor activation.
D000320:Adrenergic Fibers / Q000187:drug effects; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007275:Injections, Intravenous; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018002:Receptors, Bradykinin / Q000187:drug effects*; D017783:Superior Cervical Ganglion / Q000187:drug effects*
D008070:Lipopolysaccharides; D018002:Receptors, Bradykinin; D001920:Bradykinin