Urinary nitrate excretion is increased in patients with rheumatoid arthritis and reduced by prednisolone.
OBJECTIVE
To determine daily production of nitric oxide (NO) measured as urinary nitrate excretion, and the effect of prednisolone in patients with rheumatoid arthritis (RA).


METHODS
Twenty four hour urinary nitrate was measured by gas chromatography in 10 patients with RA, before and two to four weeks after commencement of prednisolone 0.5 mg/kg body weight, and in 18 healthy controls.


RESULTS
Before the start of prednisolone treatment the urinary nitrate excretion in patients with RA was 2.7-fold greater (p < 0.001) than that in healthy volunteers. After prednisolone it decreased significantly, by 28%, at which time inflammatory activity (as indicated by C reactive protein, erythrocyte sedimentation rate, joint count, and early morning stiffness) was also reduced considerably. Despite this decrease, the urinary nitrate excretion in patients with RA remained twice that in the control group (p < 0.05).


CONCLUSIONS
Our data suggest that the endogenous production of NO is enhanced in patients with RA. Furthermore, the results indicate that, in parallel with suppression of inflammation, this increased NO synthesis could be reduced by prednisolone treatment.
D000328:Adult; D000368:Aged; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000188:drug therapy / Q000652:urine*; D001799:Blood Sedimentation; D002097:C-Reactive Protein / Q000378:metabolism; D002849:Chromatography, Gas; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009566:Nitrates / Q000652:urine*; D009569:Nitric Oxide / Q000096:biosynthesis; D011239:Prednisolone / Q000627:therapeutic use*; D012720:Severity of Illness Index
D000893:Anti-Inflammatory Agents; D009566:Nitrates; D009569:Nitric Oxide; D002097:C-Reactive Protein; D011239:Prednisolone