Removal of hydroxyl radical increases nitric oxide generators-induced [3H]GABA release from mouse cerebral cortical neurons.
We investigated the effect of removal of hydroxyl radical on nitric oxide (NO)-induced [3H]GABA release from cerebral cortical neurons. NO generators, S-nitroso-N-acetylpenicillamine and sodium nitroprusside, significantly increased [3H]GABA release, which was completely abolished by hemoglobin. These results indicate that the release of [3H]GABA evoked by these NO generators is mediated by NO formation. N,N'-Dimethylthiourea (DMTU), a hydroxyl radical scavenger, dose-dependently stimulated the increase of NO-induced [3H]GABA release. Similarly, other hydroxyl radical scavengers such as uric acid and mannitol, significantly enhanced the NO-induced [3H]GABA release. On the other hand, each hydroxyl radical scavenger alone showed no effect on [3H]GABA release in the absence of NO generators. These results indicate that the removal of hydroxyl radical enhances NO-evoked [3H]GABA release from cerebral cortical neurons.
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism*; D008353:Mannitol / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D009569:Nitric Oxide / Q000494:pharmacology*; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology; D014527:Uric Acid / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D016166:Free Radical Scavengers; D014527:Uric Acid; D009569:Nitric Oxide; D017665:Hydroxyl Radical; D008353:Mannitol; D005680:gamma-Aminobutyric Acid; C038983:1,3-dimethylthiourea; D013890:Thiourea