title: Characteristics and possible mechanisms of low-Na+ induced contractions in rat aorta.
abstract: The influence of reducing external Na+ concentration ([Na+]ex) upon vascular smooth muscle contractility was investigated using the rat isolated aorta. NaCl from the physiological saline solution (PSS) was replaced with either choline-Cl, sucrose, or LiCl to give the following [Na+]ex (mM): 115, 85, 55, and 25 (115NaPSS to 25NaPSS). Small reductions in [Na+]ex (115NaPSS) induced a biphasic contraction, comparable in amplitude with the control one induced by phenylephrine 10(-6) M. Elimination of the endogenous catecholamine participation using either phentolamine 10(-5) M or guanethidine 3.10(-6) M similarly reduces these contractions to 25% (sucrose replacement). A similar relaxing effect was obtained with D600 10(-5) M, an antagonist of the voltage operated Ca2+ channels (25-30% residual tension for all the substitutes). Large reductions in [Na+]ex (25NaPSS) induced contractions comparable in amplitude and shape, but less sensitive to phentolamine and guanethidine (residual tension 65-75%, sucrose replacement) and insensitive to D600 (all the substitutes). The Na+/K+ ATPase inhibitor ouabain (10(-4) M) elicited slowly developing contractions, the amplitude being 115% of the phenylephrine 10(-6) M control. Phenylephrine further contracted the 115NaPSS precontracted preparations, but was significantly less effective in 25NaPSS, although the precontraction levels were similar for the same substitute used. The amplitude of the superimposed phenylephrine contractions exhibited [Na+]ex dependence. Phenylephrine 10(-6) M failed to further contract the ouabain 10(-4) M precontracted rings. We conclude that relatively small reductions in [Na+]ex are able to induce contractions of rat aorta primarily through release of endogenous catecholamines, probably through neural Na+/Ca2+ exchange. Larger reductions in [Na+]ex appear to cause contraction through muscular Na+/Ca2+ exchange.
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects*; D002794:Choline / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005711:Gallopamil / Q000494:pharmacology; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000494:pharmacology*; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors
chemical_list: D005711:Gallopamil; D013395:Sucrose; D010042:Ouabain; D012964:Sodium; D002794:Choline