Cloning and characterization of an ATPase gene from Pneumocystis carinii which closely resembles fungal H+ ATPases.
A gene encoding a P-type cation translocating ATPase was cloned from a genomic library of rat-derived Pneumocystis carinii. The nucleotide sequence of the gene contains a 2781 base-pair open reading frame that is predicted to encode a 101,401 dalton protein composed of 927 amino acids. The P. carinii ATPase protein (pcal) is 69-75% identical when compared with eight proton pumps from six fungal species. The Pneumocystis ATPase is less than 34% identical to ATPase proteins from protozoans, vertebrates or the Ca++ ATPases of yeast. The P. carinii ATPase contains 115 of 121 residues previously identified as characteristic of H+ ATPases. Alignment of the Pneumocystis and fungal proton pumps reveals five homologous domains specific for fungal H+ ATPases.
D000251:Adenosine Triphosphatases / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004271:DNA, Fungal / Q000302:isolation & purification; D005800:Genes, Fungal; D015698:Genomic Library; D008969:Molecular Sequence Data; D011010:Pneumocystis / Q000201:enzymology* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D006180:Proton-Translocating ATPases / Q000096:biosynthesis / Q000235:genetics*; D012331:RNA, Fungal / Q000302:isolation & purification; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D004271:DNA, Fungal; D012331:RNA, Fungal; D000251:Adenosine Triphosphatases; D006180:Proton-Translocating ATPases