title: Disruption of reconstituted nucleosomes. The effect of particle concentration, MgCl2 and KCl concentration, the histone tails, and temperature.
abstract: We find that reconstituted nucleosome cores containing specific DNA sequences dissociate on dilution. This disruption of histone-DNA contacts leading to the release of free DNA is facilitated by the presence of the core histone tails, MgCl2 (5 mM), KCl (60 mM), and temperatures above 0 degree C. Under reaction conditions that are commonly used to assess trans-acting factor access to nucleosomal DNA, histone-DNA contacts are on the threshold of instability. We demonstrate how dilution of reconstituted nucleosomes containing a TATA box can facilitate TBP access to DNA.
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D003850:Deoxyribonuclease I; D004912:Erythrocytes / Q000648:ultrastructure; D006657:Histones / Q000737:chemistry / Q000378:metabolism*; D015636:Magnesium Chloride / Q000494:pharmacology*; D009707:Nucleosomes / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology*; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D016385:TATA Box; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000378:metabolism; D014981:Xenopus
chemical_list: D006657:Histones; D009707:Nucleosomes; D012341:RNA, Ribosomal, 5S; D011994:Recombinant Proteins; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D015636:Magnesium Chloride; D011189:Potassium Chloride; D004247:DNA; D003850:Deoxyribonuclease I