Hucolin, a new corticosteroid-binding protein from human plasma with structural similarities to ficolins, transforming growth factor-beta 1-binding proteins.
In order to study the cortisol-binding factors in blood, human plasma was applied to a 11 beta-hydroxy-3-oxo-4-androstene-17 beta-carboxyaminoethylamine-(HACA) 1,4-butanediol diglycidyl ether-Sepharose column. Elution of the column with cortisol buffer produced two protein peaks, the minor peak yielded a protein complex of molecular weight approximately 200 kDa, subsequently termed hucolin. SDS-PAGE analysis under reducing and non-reducing conditions revealed hucolin was a disulphide-linked complex of 35-kDa and 75-kDa subunits. Twenty-five amino acid residues of the N-terminus of the 35-kDa subunit were determined and homology searches revealed an 88% sequence identity with the N-terminal region of beta-ficolin, a transforming growth factor-beta 1 (TGF-beta 1)-binding protein purified from porcine uterus.
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D053779:Latent TGF-beta Binding Proteins; D037102:Lectins; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014156:Transcortin / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D001616:beta-Galactosidase / Q000737:chemistry; D000097604:Ficolins
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D053779:Latent TGF-beta Binding Proteins; D037102:Lectins; D014156:Transcortin; D016212:Transforming Growth Factor beta; D001616:beta-Galactosidase; D000097604:Ficolins