Comparison of the solid-matrix luminescence properties of benzo(a)pyrene-DNA adducts on alpha-cyclodextrin/NaCl and trehalose/NaCl matrices.
The solid-matrix luminescence properties of (+/-)-trans-7,8-dihydroxy-anti-9, 10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene-DNA ([+/-]-anti-BPDE-DNA) adducts were compared on alpha-cyclodextrin (CD)/NaCl and trehalose/NaCl solid matrices. Both the optimum composition for the solid matrices and the best solvent system were obtained experimentally for acquiring the maximum room-temperature fluorescence (RTF) and room-temperature phosphorescence (RTP) signals for the (+/-)-anti-BPDE-DNA. Most of the solid-matrix RTF and RTP data were obtained at 296 K and 93 K for (+/-)-anti-BPDE-DNA adducts adsorbed on 1% alpha-CD/NaCl and 80% trehalose/NaCl. The RTF signals were strong for (+/-)-BPDE-DNA adducts on both solid matrices, but RTP was only obtained on the trehalose/NaCl solid matrices with the 80% trehalose yielding the strongest RTP signal for (+/-)-anti-BPDE-DNA. The fluorescence lifetime data for (+/-)-anti-BPDE-DNA gave two components on 1% alpha-CD/NaCl. For 80% trehalose/NaCl, three components were revealed, but two components were obtained with 80% trehalose/NaCl after ether extraction of the solid matrix. The third component was ascribed to the formation of the tetrols from (+/-)-anti-BPDE-DNA adducts during the drying step in the sample preparation of 80% trehalose/NaCl. The results give the first reported data on the solid-matrix luminescence of the (+/-)-anti-BPDE-DNA adducts. These results should be of considerable interest not only from an analytical viewpoint but as a new means of studying the luminescence characteristics of the adducts.
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000737:chemistry; D001564:Benzo(a)pyrene / Q000737:chemistry*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003505:Cyclodextrins / Q000737:chemistry*; D018736:DNA Adducts / Q000737:chemistry*; D049449:Luminescence; D012965:Sodium Chloride / Q000737:chemistry*; D014199:Trehalose / Q000737:chemistry*; D047391:alpha-Cyclodextrins
D003505:Cyclodextrins; D018736:DNA Adducts; D047391:alpha-Cyclodextrins; C043931:benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA; C056612:benzo(a)pyrene-DNA adduct; D001564:Benzo(a)pyrene; D012965:Sodium Chloride; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D014199:Trehalose; C032613:alpha-cyclodextrin