Solid-phase synthesis of a nucleopeptide from the linking site of adenovirus-2 nucleoprotein, -Ser(p5'CATCAT)-Gly-Asp-. Convergent versus stepwise strategy.
The synthesis of a nucleopeptide with the sequence -Ser(p5'CATCAT)-Gly-Asp- has been undertaken by either convergent or stepwise solid-phase strategies, both of which use base-labile permanent protecting groups. The coupling of phosphitylated protected peptides onto oligonucleotide-resins did not afford the desired nucleopeptide, which was nevertheless obtained after oligonucleotide elongation at the hydroxyl group of the resin-bound peptide and deprotection under mild basic conditions. A preliminary study on the stability of different nucleopeptides to bases is also reported.
D000260:Adenoviruses, Human / Q000737:chemistry; D000595:Amino Acid Sequence; D000641:Ammonia / Q000494:pharmacology; D002254:Carbonates / Q000494:pharmacology; D003852:Deoxyribonucleoproteins / Q000138:chemical synthesis* / Q000187:drug effects; D018020:Lithium Compounds / Q000494:pharmacology; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D011188:Potassium / Q000494:pharmacology; D012694:Serine / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry
D002254:Carbonates; D003852:Deoxyribonucleoproteins; D018020:Lithium Compounds; D009838:Oligodeoxyribonucleotides; D014764:Viral Proteins; D012694:Serine; D000641:Ammonia; C037593:potassium carbonate; C028467:lithium hydroxide; D011188:Potassium