The use of concanavalin A crossed immuno-affinoelectrophoresis to detect hormone-associated variations in alpha 1-acid glycoprotein.
alpha 1-Acid glycoprotein produces three peaks on crossed immuno-affinoelectrophoresis with concanavalin A in the first dimension, which is indicative of a degree of heterogeneity in the carbohydrate portion of this plasma glycoprotein. A different pattern, however, showing relatively less concanavalin A-binding material, is found in association with and after an increase in female sex hormone levels. Our observations strongly suggest a prolonged hormonal effect on the carbohydrate composition of alpha 1-acid glycoprotein, which may alter the metabolism or the function of the protein.
D003208:Concanavalin A; D004967:Estrogens / Q000502:physiology*; D005260:Female; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D009961:Orosomucoid / Q000032:analysis*; D011247:Pregnancy
D004967:Estrogens; D009961:Orosomucoid; D003208:Concanavalin A