Astrocyte membrane Na+, K(+)-ATPase and Mg(2+)-ATPase as targets of organic solvent impact.
Astrocytes serve to maintain the proper homeostatic environment for neurons. In these regulations, the astrocyte membrane ATPase plays an important role. In the present study, the astrocyte were cultured in the modified Minimum Essential Medium (MEM), and the isolated cell membranes were exposed to solvents at different concentrations (3, 6 and 9 mM) for one hour. The activities of the membrane-bound Na+, K(+)-ATPase and Mg(2+)-ATPase were determined after exposure to aromatic, alicyclic and aliphatic hydrocarbons. Aromatic hydrocarbons decreased the enzyme activities dose-dependently according to their lipid solubilities. The slight enzyme-inhibiting effect of aliphatic and alicyclic hydrocarbons was not dependent on the lipophilicity of these solvents. Their molecular structure may have determined the solvent impact. In the present study, the activity of ouabain sensitive Na+, K(+)-ATPase in astrocytes was only 20-15% of the total ATPase activity, and in neurons 45-55%. The enzyme inhibition caused by organic solvents may disturb the homeostatic regulatory functions of astrocytes and thus have a toxic effect on the CNS.
D000818:Animals; D001253:Astrocytes / Q000201:enzymology*; D001555:Benzene Derivatives / Q000494:pharmacology; D001921:Brain / Q000196:embryology; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors*; D002462:Cell Membrane / Q000201:enzymology*; D002478:Cells, Cultured; D006838:Hydrocarbons / Q000494:pharmacology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D012997:Solvents; D020058:Styrene; D013343:Styrenes / Q000494:pharmacology; D014992:Xylenes / Q000494:pharmacology
D001555:Benzene Derivatives; D006838:Hydrocarbons; D012997:Solvents; D013343:Styrenes; D014992:Xylenes; D020058:Styrene; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; C004912:ethylbenzene