In vitro antimycobacterial activity of a new quinolone, levofloxacin (DR-3355).
OBJECTIVE
To examine in vitro antimycobacterial activity of levofloxacin.


METHODS
Minimum inhibitary concentrations (MICs) of levofloxacin for various mycobacterial species were determined by the agar dilution method using 7H11 medium and compared with those of ofloxacin. Antimicrobial activity of levofloxacin against Mycobacterium tuberculosis and M. intracellulare phagocytosed in murine peritoneal macrophages was measured in terms of reducing cell-associated bacterial colony forming units (CFUs).


RESULTS
MICs of levofloxacin against M. tuberculosis, M. kansasii, M. marinum, M. scrofulaceum, M. avium, M. intracellulare, M. fortuitum, and M. chelonae were 2 to 4 times lower than those of ofloxacin. Levofloxacin exhibited higher efficacy in reducing bacterial CFUs in macrophages than ofloxacin.


CONCLUSIONS
Levofloxacin possessed more potent in vitro antimycobacterial activities as compared to that of ofloxacin.
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D005260:Female; D066298:In Vitro Techniques; D064704:Levofloxacin; D017737:Macrophages, Peritoneal / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000187:drug effects*; D015269:Mycobacterium avium Complex / Q000187:drug effects; D009169:Mycobacterium tuberculosis / Q000187:drug effects; D015242:Ofloxacin / Q000494:pharmacology*
D000890:Anti-Infective Agents; D064704:Levofloxacin; D015242:Ofloxacin