title: Yeast two-hybrid system to detect protein-protein interactions with Rho GTPases.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D004268:DNA-Binding Proteins; D004926:Escherichia coli; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000096:biosynthesis / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D020690:GTPase-Activating Proteins; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D011485:Protein Binding; D014176:Protein Biosynthesis; D017368:Protein Prenylation; D011506:Proteins / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000378:metabolism; D020742:rhoA GTP-Binding Protein
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D020690:GTPase-Activating Proteins; D007202:Indicators and Reagents; D011506:Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D001616:beta-Galactosidase; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020742:rhoA GTP-Binding Protein