Expression of the T antigen on a T-lymphoid cell line, supT1.
We have measured glycosyltransferase activities of SupT1 cells, a T-lymphoid cell line shown to react with autoantibodies in the sera of many HIV patients. Since considerable alpha-N-acetylgalactosaminyl-transferase and beta 1, 3 galactosyltransferase activities were found in SupT1 cells, at least the O-glycan core 1 structure can probably be synthesized. FACS analysis using an anti-T monoclonal antibody showed expression of the T antigen (Gal beta 1-3 GalNAc). Glycoproteins with the T antigen were isolated by immunoprecipitation with the anti-T antibody from a SupT1 cell lysate labelled metabolically with 3H-glucosamine and then analysed by SDS-PAGE. It was revealed that the precipitate contained a glycoprotein with a molecular weight corresponding to that of leukosialin. O-glycans were prepared from the immunoprecipitate by alkaline-borohydride treatment and then fractionated on Bio-Gel P-2, GalNAcOH and Gal-GalNAcOH being identified inter alia. These results suggest that an anti-T antibody may be included in the autoantibodies found in HIV-1 infected individuals.
D015703:Antigens, CD / Q000097:blood*; D000957:Antigens, Viral, Tumor / Q000097:blood*; D001323:Autoantibodies / Q000097:blood; D002240:Carbohydrate Sequence; D002460:Cell Line; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D051917:Leukosialin; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000097:blood; D012795:Sialoglycoproteins / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D015703:Antigens, CD; D000957:Antigens, Viral, Tumor; D001323:Autoantibodies; D051917:Leukosialin; D011134:Polysaccharides; C465014:SPN protein, human; D012795:Sialoglycoproteins