title: Effective method for discriminating between oral bacterial and human alkaline phosphatase activity.
abstract: Alkaline phosphatase (ALPase) activity was quantitatively compared in various kinds of oral bacteria. High ALPase activity was detected in 3 species of periodontal bacteria, Porphyromonas gingivalis, Prevotella intermedia and Capnocytophaga sputigena. The ALPase activity detected in these bacteria was almost completely inhibited in the presence of 1% sodium dodecyl sulfate (SDS). By contrast, the activity of mammalian ALPase isoenzymes was not inhibited at all even in the presence of 1% SDS. These results indicate that the ALPase assay in combination with 1% SDS can identify the origin of ALPase detected in gingival crevicular fluid as being from bacteria or from a host response. Clinical examination with adult periodontitis revealed that ALPase activity in gingival crevicular fluid from the patients consisted of a combination of SDS-sensitive and SDS-resistant activities. These findings indicate that ALPase activity detected in gingival crevicular fluid originates not only from bacteria but also from a host response.
mesh_terms: D000328:Adult; D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects / Q000201:enzymology; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000187:drug effects / Q000201:enzymology*; D002206:Capnocytophaga / Q000187:drug effects / Q000201:enzymology; D004796:Clinical Enzyme Tests; D005883:Gingival Crevicular Fluid / Q000201:enzymology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D010518:Periodontitis / Q000175:diagnosis* / Q000201:enzymology / Q000382:microbiology; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000201:enzymology; D018713:Prevotella intermedia / Q000187:drug effects / Q000201:enzymology; D012680:Sensitivity and Specificity; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013045:Species Specificity; D013291:Streptococcus / Q000187:drug effects / Q000201:enzymology; D013501:Surface-Active Agents / Q000494:pharmacology*
chemical_list: D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D000469:Alkaline Phosphatase