title: Negative charge distribution and density on the surface of oxygenated normal and sickle red cells.
abstract: Negative charges on the external surface of red cells were visualized by colloidal iron hydroxide labelling of 50% of the membrane area after osmotic hemolysis and glutaraldehyde fixation. Counts were made over randomly selected areas on electron micrographs at 350,000 x magnification. Statistical analyses showed that at the 95% level of confidence there was no significant difference between oxygenated normal (AA) and sickle (SS) cells in either the distribution or the density of negative charges.
mesh_terms: D000755:Anemia, Sickle Cell / Q000097:blood*; D004910:Erythrocyte Membrane / Q000378:metabolism* / Q000648:ultrastructure; D004912:Erythrocytes / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D007501:Iron / Q000378:metabolism; D009439:Neuraminidase / Q000494:pharmacology; D010100:Oxygen / Q000378:metabolism*
chemical_list: D007501:Iron; D009439:Neuraminidase; D010100:Oxygen