Molecular cloning of a human polypeptide related to yeast sds22, a regulator of protein phosphatase-1.
sds22 is a regulatory polypeptide of protein phosphatase-1 that is required for the completion of mitosis in both fission and budding yeast. We report here the cDNA cloning of a human polypeptide that is 46% identical to yeast sds22. The human homolog of sds22 consists of 360 residues, has a calculated molecular mass of 41.6 kDa and shows a tandem array of 11 leucine-rich repeat structures of 22 residues. Northern analysis revealed a major transcript of 1.39 kb in all 8 investigated human tissues. sds22 was detected by western analysis in both the cytoplasm and the nucleus of rat liver cells as a polypeptide of 44 kDa.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D002467:Cell Nucleus / Q000378:metabolism*; D016384:Consensus Sequence; D003600:Cytosol / Q000378:metabolism; D017931:DNA Primers; D005260:Female; D005656:Fungal Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins; D009928:Organ Specificity; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D054645:Protein Phosphatase 1; D051381:Rats; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D018797:Cell Cycle Proteins; D017931:DNA Primers; D005656:Fungal Proteins; D009687:Nuclear Proteins; C509751:PPP1R7 protein, human; C509753:Ppp1r7 protein, rat; D029702:Schizosaccharomyces pombe Proteins; C066843:sds22 protein, S pombe; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1