Biochemical, histochemical and cell biological investigations on the actions of mistletoe lectins I, II and III with human breast cancer cell lines.
Cytotoxicity of the mistletoe lectins I, II and III towards six human breast cancer cell lines was assessed using the Mossman assay. In addition, binding of the three mistletoe lectins to the separated membrane glycoproteins of these cell lines, the binding and uptake of these lectins into the cells in tissue culture and the binding of the lectins to histological preparations of these cell lines were analysed. The results indicate that there are quantitative differences concerning the toxicity of these three lectins towards the different cell lines. Furthermore, the lectin binding pattern in the cell lines differed. In Western blots, several membrane glycoproteins were labelled by the lectins. Our results indicate subtle differences between the three lectins with regard to the parameters mentioned above; however, the toxicity of all three lectins from mistletoe is so similar that they all seem suitable for the construction of immunotoxins.
D001943:Breast Neoplasms / Q000737:chemistry*; D003601:Cytotoxicity Tests, Immunologic; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins / Q000378:metabolism / Q000627:therapeutic use*; D008562:Membrane Glycoproteins / Q000378:metabolism; D028321:Plant Preparations; D010940:Plant Proteins; D011485:Protein Binding; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological / Q000378:metabolism / Q000627:therapeutic use*; D014407:Tumor Cells, Cultured
D037102:Lectins; D008562:Membrane Glycoproteins; D028321:Plant Preparations; D010940:Plant Proteins; D054790:Ribosome Inactivating Proteins, Type 2; D014118:Toxins, Biological; C009674:ribosome inactivating protein, Viscum