Binding of 17-alpha-methyltestosterone in vitro to human sex hormone binding globulin and rat ventral prostate androgen receptors.
We measured the relative binding affinity (RBA) of 17-alpha-methyltestosterone (MET) for the rat prostate androgen receptor and human sex hormone-binding globulin (SHBG) in vitro. The reference compound in both instances was dihydrotestosterone (DHT), the RBA of which was set at 100%. The RBA of methyltestosterone was 44% for the androgen receptor, but 11% for SHBG. The androgen receptor, therefore, would appear to recognize a different part of an androgen compared to the recognition site on SHBG. The difference in RBAs may amplify the biologic action of methyltestosterone.
D000818:Animals; D013196:Dihydrotestosterone / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D008777:Methyltestosterone / Q000378:metabolism*; D011467:Prostate / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D011944:Receptors, Androgen / Q000378:metabolism*; D012738:Sex Hormone-Binding Globulin / Q000378:metabolism*
D011944:Receptors, Androgen; D012738:Sex Hormone-Binding Globulin; D013196:Dihydrotestosterone; D008777:Methyltestosterone