Molecular cloning of a novel laminin chain, alpha 5, and widespread expression in adult mouse tissues.
We have identified a fifth member of the alpha subfamily of vertebrate laminin chains. Sequence analysis revealed a close relationship of alpha 5 to the only known Drosophila alpha chain, suggesting that the ancestral alpha gene was more similar to alpha 5 than to alpha 1-4. Analysis of RNA expression showed that alpha 5 is widely expressed in adult tissues, with highest levels in lung, heart, and kidney. Our results suggest that alpha 5 may be a major laminin chain of adult basal laminae.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D006801:Humans; D007668:Kidney / Q000378:metabolism; D007797:Laminin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D012313:RNA / Q000235:genetics / Q000378:metabolism
D018076:DNA, Complementary; D007797:Laminin; C097043:laminin alpha5; D012313:RNA