A new method for direct detection of Listeria monocytogenes from foods by PCR.
The preparation of DNA by alcohol precipitation in the presence of Na1 was used to enable the direct detection of Listeria monocytogenes in the amount of 10(3) CFU/0.5 g of sample. This procedure produces PCR-quality DNA directly from foods, such as soft cheese and minced meat.
D000818:Animals; D001483:Base Sequence; D002611:Cheese / Q000382:microbiology; D011232:Chemical Precipitation; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000209:etiology; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics* / Q000302:isolation & purification*; D008088:Listeriosis / Q000209:etiology; D008460:Meat / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012974:Sodium Iodide
D017931:DNA Primers; D004269:DNA, Bacterial; D012974:Sodium Iodide