Effects of testosterone on the rat renal medullary vasopressin receptor concentration and the antidiuretic response.
The renal concentrating ability declines with age in humans and animals. Studies suggest that the concentrating defect is due to a decrease in renal vasopressin sensitivity. With ageing, expression of the renal vasopressin V2 receptor in rat is impaired; the normal receptor expression is restored by testosterone treatment. The effect of testosterone on the renal sensitivity to vasopressin was investigated in young rats. Male rats after orchidectomy and chronic antiandrogen cyproterone acetate treatment, and female rats after chronic testosterone phenylpropionate treatment, were used. The plasma arginine-vasopressin (AVP) and testosterone concentrations, and the antidiuretic responses to AVP and the V2 agonist deamino-[8-D-arginine]-vasopressin (dDAVP) after volume loading were measured, and the renal [3H]AVP binding density was determined. The plasma AVP level decreased slightly, but not significantly, in male rats after orchidectomy and cyproterone acetate treatment, but did not alter in female rats after testosterone treatment. The AVP and dDAVP sensitivities decreased in male rats after orchidectomy and cyproterone acetate administration, and increased in female rats treated with testosterone, as compared with the animals with a normal gonadal function. [3H]AVP binding to the renal inner medullary membranes was decreased following orchidectomy or antiandrogen treatment in male rats, and increased in testosterone-treated female rats. The results suggest that testosterone may play a physiological role in maintenance of the V2 vasopressin receptor expression and hence in the normal urinary concentrating ability in rat.
D000726:Androgen Antagonists / Q000494:pharmacology; D000818:Animals; D001127:Arginine Vasopressin / Q000097:blood; D017373:Cyproterone Acetate / Q000494:pharmacology; D003894:Deamino Arginine Vasopressin / Q000494:pharmacology; D004231:Diuresis / Q000187:drug effects*; D005260:Female; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009919:Orchiectomy; D051381:Rats; D017208:Rats, Wistar; D017483:Receptors, Vasopressin / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000031:analogs & derivatives / Q000097:blood / Q000494:pharmacology*; D043343:Testosterone Propionate / Q000031:analogs & derivatives
D000726:Androgen Antagonists; D017483:Receptors, Vasopressin; D001127:Arginine Vasopressin; D013739:Testosterone; D017373:Cyproterone Acetate; C035458:testosterone 17-phenylpropionate; D003894:Deamino Arginine Vasopressin; D043343:Testosterone Propionate