[Simultaneous assay of Ca(2+)-ATPase and Na+, K(+)-ATPase activities of hemolysate by malachite green colorimetric method].
Activities of Ca(2+)-ATPase and Na+, K(+)-ATPase of hemolysate were simultaneously determined by a colorimetric method with malachite green dye. In the presence of Ca2+, EGTA or ouabain, inorganic phosphate released from ATP by hypotonic saponin-treated red cell lysate was used to estimate the activities of Ca(2+)-ATPase and Na2+, K(+)-ATPase. The method is simple, sensitive and stable for at least 4 h. The effects of concentration of saponin and Ca2+, the storage of hemolysate, and the activator and inhibitor of the enzymes on activity were discussed.
D000252:Calcium-Transporting ATPases / Q000097:blood*; D003124:Colorimetry; D004396:Coloring Agents; D004912:Erythrocytes / Q000201:enzymology*; D006461:Hemolysis; D006801:Humans; D012394:Rosaniline Dyes; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*
D004396:Coloring Agents; D012394:Rosaniline Dyes; C005095:malachite green; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase