title: In vitro binding assay for interactions of Rho and Rac with GTPase-activating proteins and effectors.
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism*; D020690:GTPase-Activating Proteins; D006160:Guanosine Triphosphate / Q000378:metabolism; D007202:Indicators and Reagents; D007700:Kinetics; D008322:Mammals; D016297:Mutagenesis, Site-Directed; D010761:Phosphorus Radioisotopes; D017354:Point Mutation; D011485:Protein Binding; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D011865:Radioisotope Dilution Technique; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism; D014316:Tritium; D020744:rac GTP-Binding Proteins
chemical_list: D020690:GTPase-Activating Proteins; D007202:Indicators and Reagents; D010761:Phosphorus Radioisotopes; D011506:Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014316:Tritium; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins; D020744:rac GTP-Binding Proteins