A new sensitive and simple method for detection of catecholamines from adrenal chromaffin cells.
A biosensor was used for the analysis of catecholamines in media and lysates of cultured bovine adrenal chromaffin cells. The sensor is composed of coimmobilised laccase and glucose dehydrogenase coupled with an oxygen electrode, using the catalytic effect of cate cholamines for glucose oxidation in this system. The analysis time is almost 5 min. The correlation between the biosensor and HPLC determination is 0.99.
D000313:Adrenal Medulla / Q000737:chemistry / Q000166:cytology; D000818:Animals; D015374:Biosensing Techniques; D002395:Catecholamines / Q000032:analysis*; D002417:Cattle; D002838:Chromaffin System / Q000737:chemistry* / Q000166:cytology; D002851:Chromatography, High Pressure Liquid; D005948:Glucose Dehydrogenases; D042845:Laccase; D010088:Oxidoreductases
D002395:Catecholamines; D010088:Oxidoreductases; D005948:Glucose Dehydrogenases; D042845:Laccase