Influence of monoglucosyldiacylglycerol and monoacylmonoglucosyldiacylglycerol on the lipid bilayer of the membrane from Acholeplasma laidlawii strain A-EF22.
The ability for 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol (MGlcDAG) and 1,2-diacyl-3-O-(6-O-acyl-(alpha-D-glucopyranosyl))-sn-glycerol (MAMGlcDAG) to induce non-lamellar phases in a lipid mixture with an in vivo composition, prepared from Acholeplasma laidlawii membranes, has been investigated. The phase transition temperatures from lamellar to non-lamellar structures were studied with varying fractions of MGlcDAG and MAMGlcDAG. The transition temperature decreased from 73 +/- 2 degrees C for 20 mol% MGlcDAG to 43 +/- 1 degree C for 63 mol% MGlcDAG, in lipid mixtures where the other lipids are the native bilayer-forming lipids. MAMGlcDAG behaved differently and the phase transition temperatures were found to be almost constant and between 51-53 degrees C as the fraction of MAMGlcDAG varied between 11-45 mol%. It was also found that MAMGlcDAG can only be solubilized in low concentrations in the lipid bilayer, which is in good agreement with the fractions of MAMGlcDAG found in the membrane of A. laidlawii. Higher concentrations of MAMGlcDAG resulted in phase separations of lamellar liquid crystalline and gel/crystalline phases. It is concluded that MAMGlcDAG is far more capable than MGlcDAG to induce non-lamellar structures at lower concentrations. The results are discussed in terms of the model of lipid regulation previously proposed by this laboratory (Lindblom, G., Hauksson, J.B., Rilfors, L., Bergenståhl, B., Wieslander, A. and Eriksson, P.O. (1993) J. Biol. Chem. 268, 16198-16207), and the importance for the bilayer stability in cell membranes. It is proposed that the phase behaviour of the membrane lipids has far-reaching consequences for membrane function.
D000128:Acholeplasma laidlawii / Q000737:chemistry / Q000187:drug effects*; D002462:Cell Membrane / Q000737:chemistry / Q000187:drug effects*; D006017:Glycolipids / Q000494:pharmacology*; D008051:Lipid Bilayers; D009682:Magnetic Resonance Spectroscopy
C086507:3-O-acyl-monoglucosyl diacylglycerol; D006017:Glycolipids; D008051:Lipid Bilayers; C014571:glucosyl diacylglycerol