[Characteristics of the RFLP marker of the D13S25 locus on human chromosome 13: mapping of SspI sites in the pH2-42 probe].
Analysis of probe pH2-42 of locus D13S25 in genomic DNA in Moscow population has been made by PCR-RFLP using restriction endonuclease SspI. Localization of additional SspI-site at position 1240 (which has been revealed earlier by sequencing of pH2-42) was confirmed. This SspI restriction site was present in all representatives of the population, thus being not polymorphic. Consequently establishment of identity of additional site at position 1240 with polymorphic SspI site in D13S25 studied in previous investigations, requires additional data from another populations. Possible localization of polymorphic SspI-site in locus D13S25 is discussed.
D001483:Base Sequence; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D017931:DNA Primers; D015342:DNA Probes; D015252:Deoxyribonucleases, Type II Site-Specific; D005819:Genetic Markers; D006527:Hepatolenticular Degeneration / Q000175:diagnosis / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D009031:Moscow; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D017931:DNA Primers; D015342:DNA Probes; D005819:Genetic Markers; C061568:endodeoxyribonuclease SspI; D015252:Deoxyribonucleases, Type II Site-Specific
K voprosu o kharakteristike PDRF-markera lokusa D13S25 13-ĭ khromosomy cheloveka: lokalizatsiia SspI-saĭtov v probe pH2-42.