Comparison of reversed-phase and ion-pair chromatography for the determination of strychnine in animal tissues.
Ion-pair and reversed-phase high-performance liquid chromatography (HPLC) were evaluated for quantification of strychnine in mountain beaver tissues. Retention time shifts hindered strychnine quantification with both HPLC systems. Co-extracted free fatty acids released during storage formed ion-pairs with strychnine, resulting in increased retention by reversed-phase HPLC. Competition with co-extracted basic compounds is likely responsible for the decreased retention of strychnine by ion-pair HPLC. Following an acid-base clean-up, optimal results were obtained with reversed-phase HPLC. Ion-pair chromatography was then used for qualitative confirmation of strychnine residues.
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004064:Digestive System / Q000737:chemistry*; D007477:Ions; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*; D015203:Reproducibility of Results; D012377:Rodentia; D013056:Spectrophotometry, Ultraviolet; D013331:Strychnine / Q000032:analysis*
D007477:Ions; D013331:Strychnine