Changing nucleosome positions through modification of the DNA rotational information.
The effects of the rotational information of DNA in determining the in vitro localization of nucleosomal core particles (ncps) have been studied in the Saccharomyces cerevisiae 5S rRNA repeat gene. We have altered the distribution of the phased series of flexibility signals present on this DNA by inserting a 25-bp tract, and we have analyzed the effects of this mutation on the distribution and on the frequencies of ncps, as compared with the wild type and a reference 21-bp insertion mutant. The variation of the standard free energy of nucleosome reconstitution was determined. The results show that the DNA rotational information is a major determinant of ncps positioning, define how many rotationally phased signals are required for the formation of a stable particle, and teach how to modify their distribution through the alteration of the rotational signals.
D001483:Base Sequence; D001665:Binding Sites; D018983:DNA Footprinting; D004271:DNA, Fungal / Q000235:genetics / Q000648:ultrastructure*; D004275:DNA, Ribosomal / Q000235:genetics / Q000648:ultrastructure*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000378:metabolism; D017665:Hydroxyl Radical; D008969:Molecular Sequence Data; D005810:Multigene Family; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000648:ultrastructure*; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D009707:Nucleosomes; D012341:RNA, Ribosomal, 5S; D017665:Hydroxyl Radical; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D015252:Deoxyribonucleases, Type II Site-Specific