Inhibition of human immunodeficiency virus type 1 (HIV) gag gene expression by an antisense oligodeoxynucleotide phosphorothioate.
We have employed a cos-like monkey kidney cell line (B4.14) transfected with plasmids pCMVgag-pol-rre-r (containing the HIV gag and pol genes) and pCMVrev (containing the HIV rev gene), as a model to investigate whether antisense constructs could interfere with specific HIV gene and protein expression. We utilized an antisense construct (GP12A) directed against a non-regulatory region of the HIV genome, to transfect cells that expressed the above-mentioned HIV genes. Our results show that GP12A was able to attenuate levels of relevant HIV mRNA and gag proteins in the absence of cytotoxic effects.
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D015870:Gene Expression / Q000187:drug effects*; D015683:Gene Products, gag / Q000096:biosynthesis*; D015750:Genes, gag / Q000187:drug effects*; D015751:Genes, pol; D016679:Genome, Viral; D015497:HIV-1 / Q000187:drug effects / Q000235:genetics*; D006801:Humans; D007668:Kidney; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012045:Regulatory Sequences, Nucleic Acid; D013873:Thionucleotides; D014158:Transcription, Genetic / Q000187:drug effects
D000998:Antiviral Agents; D015683:Gene Products, gag; D016376:Oligonucleotides, Antisense; D013873:Thionucleotides