Ligands of purinergic receptors stimulate electrogenic H(+)-transport of neutrophils.
The possible role of ATP, acting as a ligand on cell surface receptor was investigated in the activation of the electrogenic H(+)-transporting pathway of porcine neutrophil granulocytes. (1) ATP brought about 2.1-fold increase in the rate of H(+)-efflux. (2) The order of potency of different nucleotides suggests, that ATP acts on P2 type purinoceptor. (3) The effect of the nucleotides was prevented by inhibition of phospholipase A2. (4) Inhibition of the metabolism of arachidonic acid (AA) via the cyclooxygenase pathway had no effect, whereas inhibition of the lipoxygenase pathway significantly enhanced H(+)-release. This is the first report about activation of the H(+)-transporter by physiological stimulator acting on the cell surface.
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology*; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D009637:Masoprocol / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000097:blood; D054467:Phospholipases A2; D006180:Proton-Translocating ATPases / Q000097:blood*; D058910:Purinergic P2 Receptor Agonists; D012265:Ribonucleotides / Q000494:pharmacology; D013552:Swine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D058910:Purinergic P2 Receptor Agonists; D012265:Ribonucleotides; D016718:Arachidonic Acid; D009240:N-Formylmethionine Leucyl-Phenylalanine; D009637:Masoprocol; D000255:Adenosine Triphosphate; D010741:Phospholipases A; D054467:Phospholipases A2; D006180:Proton-Translocating ATPases; D013755:Tetradecanoylphorbol Acetate; D007213:Indomethacin