title: Polyamines found in gingival fluid enhance the secretory and oxidative function of human polymorphonuclear leukocytes in vitro.
abstract: Many bacterial and host cells contain large amounts of polyamines that can be released at infection sites as a result of cell lysis. Consequently, the putrescine and spermidine content of gingival fluid from inflamed periodontal pockets (0.1 to 1 mM) is sharply elevated in comparison to peripheral blood. At these levels, polyamines potentiated fMet-Leu-Phe-induced Ca2+ signaling in polymorphonuclear leukocytes (PMNs) in vitro. Consistent with the essential role of Ca2+ signaling in PMN activation, secondary granule release and superoxide anion production by fMet-Leu-Phe-stimulated PMNs was enhanced in the presence of polyamines. Thus, polyamines may play a local role in modulating the antimicrobial activity of PMNs in periodontal disease.
mesh_terms: D002118:Calcium / Q000819:agonists / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D015550:Cell Degranulation / Q000187:drug effects / Q000502:physiology; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D018375:Neutrophil Activation / Q000187:drug effects / Q000502:physiology*; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D011073:Polyamines / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology; D011700:Putrescine / Q000502:physiology; D016897:Respiratory Burst / Q000502:physiology; D013095:Spermidine / Q000502:physiology; D013481:Superoxides / Q000378:metabolism
chemical_list: D011073:Polyamines; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D000252:Calcium-Transporting ATPases; D002118:Calcium; D013095:Spermidine; D011700:Putrescine