A method for in vivo [32P]phosphate labelling of testis proteins.
The direct intratesticular injection of [32P]phosphate resulted in 4-9 times more labelling of rat testis proteins compared to the conventional method of in vitro incubation. Moreover this is a simple technique requiring minimum (7-10 times less) radioactive phosphate and is less hazardous.
D000818:Animals; D007553:Isotope Labeling / Q000379:methods; D008297:Male; D010710:Phosphates / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000737:chemistry / Q000378:metabolism*; D014238:Trichloroacetic Acid
D010710:Phosphates; D010761:Phosphorus Radioisotopes; D011506:Proteins; D014238:Trichloroacetic Acid