[Purine arabinonucleoside-5'-triphosphates with substituents in the 2'-position as DNA polymerase substrates].
Analogues of araNTPs carrying an azido or aminogroup instead of the 2' hydroxyl exhibited substrate properties towards several mammalian and viral DNA polymerases. At the same time, introduction of a bulky hydrophobic DNP group into the 2' position inactivated the compounds as substrates. HSV-1 and CMV DNA polymerases were an interesting exception: they effectively incorporated the modified nucleotide residues with DNP group into the 3'-termini of the DNA chain. This is a reliable way to distinguish these enzymes from cellular DNA polymerases.
D000818:Animals; D001088:Arabinonucleotides / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003587:Cytomegalovirus / Q000201:enzymology; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D018259:Herpesvirus 1, Human / Q000201:enzymology; D008969:Molecular Sequence Data; D013056:Spectrophotometry, Ultraviolet; D013379:Substrate Specificity
D001088:Arabinonucleotides; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase
Purinovye arabinonukleozid-5'-trifosfaty s zamestiteliami v 2'-polozhenii kak substraty DNK-polimeraz.