Absorption with K562 erythroblastoma cells as a means for discrimination between auto- and alloantibodies in sera of hypersensitized renal patients.
We applied K562 erythroblastoma cell line as a substitute for autologous lymphocytes in the absorption of autoantibodies from sera of 26 hypersensitized renal patients exhibiting high degree of cytotoxic reactivity with a panel of lymphocytes from unrelated individuals (panel reactivity, PRA). All of analysed sera contained allo-reactivity before absorption. After removal of autoantibodies, 85% of the sera still contained alloantibodies whereas in 15% of the sera no alloreactivity was detectable. Absorption of high PRA sera with K562 cells and with autologous lymphoblastoid cell lines derived by transformation with Epstein-Barr virus (EBV-LCL) gave substantially the same results. As autoantibodies have reportedly no effect on kidney graft survival, the autoabsorption of high PRA sera may increase the chance of patients in whom only autoantibodies were detected to receive the transplant. Application of K562 cells, instead of autologous lymphocytes/lymphoblasts, is recommended whenever high numbers of patient's cells are not available. It may be particularly suitable for routine clinical laboratories, not equipped and prepared for derivation and propagation of EBV-LCL.
D000328:Adult; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D005260:Female; D006801:Humans; D007114:Immunization; D007163:Immunosorbent Techniques; D007518:Isoantibodies / Q000097:blood* / Q000276:immunology; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000276:immunology / Q000628:therapy; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D016131:Lymphocyte Subsets / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011247:Pregnancy; D011248:Pregnancy Complications / Q000276:immunology; D006435:Renal Dialysis; D065227:Transfusion Reaction; D014407:Tumor Cells, Cultured
D001323:Autoantibodies; D007518:Isoantibodies