title: Methodology for the assessment of lung protection. Human pulmonary artery endothelial cell preservation using haemaccel.
abstract: This investigation was designed to show an original methodology for the assessment of lung preservation and to analyze the efficacy of a low potassium polygelin solution (haemaccel [HM]) on isolated human pulmonary artery endothelial cells. The effects of HM were compared with those of low potassium dextran (LPD), Belzer (University of Wisconsin [UWS]), and Euro-Collins solutions. The viability of the endothelial cultures was assessed by means of both total protein content and recovery of metabolic cellular function expressed as the protein synthesis rate after 6 hr and 16 hr of incubation at 10 degrees C. Our results failed to show any significant difference in the total protein content for HM, LPD, and UWS, both after 6 and 16 hr of incubation; however, the Euro-Collins-preserved sample revealed a significant drop in this parameter as early as 6 hr after the start. This finding was regarded as a clear indication of cellular cytotoxicity. In contrast, the metabolism recovery capacity of the cells varied significantly between HM and UWS at 6 hr and among HM, LPD, and UWS at 16 hr; at 6 hr, however, no significant difference was observed between HM and LPD. In conclusion, HM appears to exert a more significant effect on human pulmonary artery endothelial cell metabolism recovery than do the other fluids, thus suggesting its suitability as a long-term pulmonary perfusate.
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007930:Leucine / Q000378:metabolism; D008168:Lung; D009926:Organ Preservation / Q000379:methods*; D010952:Plasma Substitutes; D011097:Polygeline; D014176:Protein Biosynthesis; D011651:Pulmonary Artery; D011865:Radioisotope Dilution Technique; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods; D014316:Tritium
chemical_list: D010952:Plasma Substitutes; D014316:Tritium; D011097:Polygeline; D007930:Leucine