title: Glucose-6-phosphate dehydrogenase from Saccharomyces cerevisiae is a glycoprotein.
abstract: A commercial preparation of glucose-6-phosphate dehydrogenase (G6PD) purified from Saccharomyces cerevisiae was subjected to PAGE analysis under both nondenaturing and denaturing conditions. The enzyme, identified by both activity staining and anti-yeast G6PD antibody immunoblotting, was shown to contain carbohydrate using the highly specific periodate-digoxigenin antidigoxigenin method which is diagnostic for glycoproteins.
mesh_terms: D003101:Collodion; D004076:Digoxigenin / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis* / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis*; D015151:Immunoblotting; D008970:Molecular Weight; D010504:Periodic Acid; D011489:Protein Denaturation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013194:Staining and Labeling
chemical_list: D006023:Glycoproteins; D010504:Periodic Acid; D003101:Collodion; D005954:Glucosephosphate Dehydrogenase; D004076:Digoxigenin