An IS903-based vector for transposon mutagenesis and the isolation of gene fusions.
A derivative of the IS903 transposon (Tn) is described that is capable of creating lacZ gene fusions upon transposition. It should find wide use as a tool for Tn mutagenesis in bacteria since it can be used both to generate mutants and to examine gene expression. The transposase-encoding gene (tnp) is located outside the Tn in the vector, thus Tn insertions into a genome are stably maintained in the absence of its cis-acting transposase (Tnp). The element carries a KmR gene allowing for the direct selection of transposition events in hosts that cannot support pBR322 plasmid replication and facilitating the subcloning of genes into which the Tn has inserted.
D003001:Cloning, Molecular; D004251:DNA Transposable Elements / Q000235:genetics*; D005822:Genetic Vectors; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000235:genetics
D004251:DNA Transposable Elements