Production of Trichoderma reesei cellulases on glucose-containing media.
The filamentous fungus Trichoderma reesei was shown to secrete active cellobiohydrolase I and the endoglucanase I catalytic core domain into the culture medium when the fungus was grown on glucose-containing medium. The expression of the proteins was driven by the promoters of the elongation factor 1 alpha, tef1, and the unidentified gene for cDNA1. The cDNA1 promoter gave the best yields. The highest amounts of cellobiohydrolase I and the endoglucanase I core, being 50 to 100 mg/liter, accounted for more than half of the total protein secreted by the fungus. The levels obtained with the tef1 promoter were 20 to 50 times lower.
D001483:Base Sequence; D043366:Cellulose 1,4-beta-Cellobiosidase; D003470:Culture Media; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014170:Transformation, Genetic; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
D003470:Culture Media; D017931:DNA Primers; D018076:DNA, Complementary; D004271:DNA, Fungal; D006026:Glycoside Hydrolases; D043366:Cellulose 1,4-beta-Cellobiosidase; D005947:Glucose