3-Methylindole (skatole) and indole production by mixed populations of pig fecal bacteria.
Pig fecal slurries converted added L-tryptophan either to indole without detectable intermediates or to 3-methylindole (skatole) via indole-3-acetate. The initial rate of production of 3-methylindole was greatest at pH 6.5 and less at pH 5.0 and 8.0; the initial rates of indole production were similar at pH 6.5 and 8.0. More than 80% of the tryptophan added was converted to 3-methylindole at pH 5.0; at pH 8.0 85% was converted to indole. Both pathways had similar Km values for tryptophan and similar maximum rates. Indole-3-carbinol and indole-3-acetonitrile completely inhibited the production of 3-methylindole from indole-3-acetate but had no effect on the reactions involving L-tryptophan.
D000818:Animals; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D007210:Indoleacetic Acids / Q000378:metabolism; D007211:Indoles / Q000378:metabolism*; D007700:Kinetics; D012862:Skatole / Q000378:metabolism*; D013552:Swine / Q000382:microbiology*; D014364:Tryptophan / Q000378:metabolism
D007210:Indoleacetic Acids; D007211:Indoles; C030737:indoleacetic acid; C030374:indole; D014364:Tryptophan; D012862:Skatole