Determination of tissue endothelin levels.
A methodology for the quantitation of tissue endothelin levels has been developed. About 85% of authentic endothelin-1 added to the tissue extract was recovered. Using this protocol, the levels of endothelin in various rat tissues were determined. In Wistar-Kyoto rats, the kidney was found to have the highest level of endothelin, 1120 pg/gm wet weight, followed by the spleen and liver. The brain contained only half as much of endothelin when compared with the kidney. This method can be utilized to assess the pathological role of endothelin in cardiovascular or renal diseases.
D000818:Animals; D016232:Endothelins / Q000032:analysis*; D006973:Hypertension / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012995:Solubility
D016232:Endothelins