Chromogenic assay for phospholipase C from Bacillus cereus.
A standard chromogenic assay for phosphatidylcholine determination has been modified in the development of a facile method for measuring the rate parameters (Km and Vmax) for phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc). The assay is applied to the determination of the Ki for a known inhibitor of PLCBc.
D001409:Bacillus cereus / Q000201:enzymology*; D007202:Indicators and Reagents; D007700:Kinetics; D010713:Phosphatidylcholines / Q000378:metabolism; D013053:Spectrophotometry / Q000379:methods; D013379:Substrate Specificity; D013997:Time Factors; D010738:Type C Phospholipases / Q000032:analysis* / Q000378:metabolism*
D007202:Indicators and Reagents; D010713:Phosphatidylcholines; C032166:1,2-hexanoylphosphatidylcholine; D010738:Type C Phospholipases