title: Sequences responsible for transcription termination of the mouse gastrin gene.
abstract: To identify the transcription termination elements in the mouse gastrin gene, we examined RNA transcripts after stable transfection of gastrin expression plasmids into the NIH3T3 cell line. The GT-repeat region at the 3'-flanking sequence of the mouse gastrin gene acted as a transcription terminator. When the GT-repeat unit was deleted from its site, the effect of termination disappeared. Further experiment, using serial deletion mutants, revealed that the 56-38 nucleotide upstream region from the GT-repeat unit also participated in transcription termination. We propose that the upstream region of the GT-repeat unit might be recognized as a pause site by the RNA polymerase II, and an abnormal DNA structure, derived from the GT-repeat unit, might function as a blockage of polymerase processivity.
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D005755:Gastrins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010443:Peptide Chain Termination, Translational; D012319:RNA Polymerase II / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D005755:Gastrins; D012319:RNA Polymerase II