Enhanced expression of tyrosine hydroxylase and aromatic L-amino acid decarboxylase in cerebellar Purkinje cells of mouse after hyperosmotic stimuli.
We examined by immunohistochemistry the effect of salt loading on the expression of tyrosine hydroxylase (TH), aromatic L-amino acid decarboxylase (AADC), and GTP cyclohydrolase I (GCH) in Purkinje cells of the mouse cerebellum. In control mice, only a few Purkinje cells were positive for TH or AADC. No Purkinje cells were stained for GCH. Drinking 2% sodium chloride for 2 weeks resulted in an increase in the number of TH- or AADC-positive Purkinje cells in the caudal vermis, paraflocculus and flocculus of the cerebellum. In contrast, no Purkinje cells were immunoreactive to GCH or L-DOPA after the salt loading. The present findings suggest that the salt loading differentially affects the expression of TH, AADC and GCH in Purkinje cells of the mouse cerebellum.
D000818:Animals; D002262:Carboxy-Lyases / Q000235:genetics*; D003681:Dehydration; D004298:Dopamine / Q000378:metabolism; D007150:Immunohistochemistry; D051379:Mice; D008815:Mice, Inbred Strains; D009995:Osmosis; D011689:Purkinje Cells / Q000276:immunology*; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics*
D014446:Tyrosine 3-Monooxygenase; D002262:Carboxy-Lyases; D004298:Dopamine