title: Clinical laboratory determination of phosphatidylglycerol: one- and two-dimensional chromatography compared.
abstract: Reportedly, determination of several phospholipids in amniotic fluid, including phosphatidylglycerol and phosphatidylinositol, in addition to lecithin and sphingomyelin improves prediction of neonatal pulmonary function. In this study, we evaluated a two-dimensional technique for separating and measuring these phospholipids and compared it with a simpler one-dimensional procedure. The two-dimensional technique was adapted to readily available commercial plates, and a preheating step was introduced to avoid shattering of the plates during charring. The Rf values, reproducibility of each technique, and the correlation between them were examined. Even though the one-dimensional technique is faster and less expensive, we recommend the two-dimensional method for clinical use because of better precision (CV for phosphatidylglycerol 15% vs 21%) and clearer results when relatively little phosphatidylglycerol is present. The one-dimensional procedure is unreliable when blood or meconium are present. In addition, interfering compounds co-migrate with phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylserine in the one-dimensional technique. Before any one-dimensional lipid separation is adopted for clinical use, it should be critically compared to the two-dimensional procedure.
mesh_terms: D000653:Amniotic Fluid / Q000032:analysis*; D002855:Chromatography, Thin Layer; D003920:Diabetes Mellitus / Q000378:metabolism; D005260:Female; D006801:Humans; D006973:Hypertension / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D011225:Pre-Eclampsia; D011247:Pregnancy; D011248:Pregnancy Complications; D011249:Pregnancy Complications, Cardiovascular; D011254:Pregnancy in Diabetics
chemical_list: D010715:Phosphatidylglycerols; D010743:Phospholipids