Evidence for a glycyl-proline transport system in ovine enterocyte brush-border membrane vesicles.
A peptide transport system in ovine enterocyte BBMV has been examined with glycyl-L-proline (Gly-Pro) as substrate. Transport is stimulated by an inwardly directed proton gradient (pH1 = 8.4; pH0 = 6.0). Gly-Pro uptake was saturable and conformed to Michaelis-Menten kinetics. Uptake was not affected by the presence of glycine but was inhibited by glycyl-L-phenylalanine (Gly-Phe). This is thought to be the first positive demonstration of the existence of a peptide transporter in the ruminant small intestine.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002458:Cell Fractionation; D004151:Dipeptides / Q000378:metabolism* / Q000494:pharmacology; D004386:Duodenum / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000378:metabolism*; D007700:Kinetics; D008871:Microvilli / Q000378:metabolism*; D012756:Sheep
D004151:Dipeptides; C015248:glycylproline; C029660:glycylphenylalanine