title: GTP-binding protein-mediated production of superoxide anion in rabbit gastric parietal cells.
abstract: We found that guanosine-5'-O-(3-thiotriphosphate) (GTP gamma S) increased superoxide production in the suspension rich in rabbit gastric parietal cells that were permeabilized by streptolysin O. In this experiment, oxidation of non-fluorescent dihydrorhodamine 123 into the fluorescent rhodamine 123 by oxygen radicals was measured. Results support that GTP gamma S closes a housekeeping C1- channel via intracellular production of superoxide anion in the parietal cell.
mesh_terms: D000818:Animals; D001426:Bacterial Proteins; D002463:Cell Membrane Permeability; D019204:GTP-Binding Proteins / Q000502:physiology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000378:metabolism*; D011817:Rabbits; D012235:Rhodamines; D013301:Streptolysins / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D012235:Rhodamines; D013301:Streptolysins; C025964:streptolysin O; C058319:dihydrorhodamine 123; D013481:Superoxides; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D019204:GTP-Binding Proteins