Detection of squamous neoplasia by fluorescence imaging comparing porfimer sodium fluorescence to tissue autofluorescence in the hamster cheek-pouch model.
BACKGROUND
Early neoplastic changes in the oral cavity may be difficult to detect. Fluorescence imaging using porphyrin-derived drugs has been used to enhance detection of neoplasia. Autofluorescence has also been used for this purpose. This paper compares autofluorescence to porfimer sodium-induced fluorescence in the detection of neoplasia in the hamster cheek-pouch model.


METHODS
Neoplasia was induced in the hamster cheek pouch by the application of 9,10-dimethyl-1,2-benzanthracene. Animals were imaged either with injection of drug (porfimer sodium) or without drug (autofluorescence). Imaging was carried out using a laser-induced fluorescence detection system. Biopsies were performed on imaged sections and histologic grades were assigned.


RESULTS
Porfimer sodium fluorescence provided 100% sensitivity and specificity in detection of neoplasia. The sensitivity and specificity with autofluorescence was 76% and 83%, respectively.


CONCLUSIONS
Autofluorescence provides an accurate means of detecting early neoplastic changes in the hamster cheek-pouch model; however, porfimer sodium imaging does improve detection rates.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000009:adverse effects; D000818:Animals; D000970:Antineoplastic Agents; D001706:Biopsy; D002273:Carcinogens / Q000009:adverse effects; D006224:Cricetinae; D004195:Disease Models, Animal; D004311:Double-Blind Method; D005453:Fluorescence; D005456:Fluorescent Dyes; D017324:Hematoporphyrin Derivative; D007834:Lasers; D008297:Male; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000187:drug effects / Q000473:pathology; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000473:pathology*; D018307:Neoplasms, Squamous Cell / Q000139:chemically induced / Q000473:pathology*; D011230:Precancerous Conditions / Q000139:chemically induced / Q000473:pathology*; D012372:ROC Curve; D012680:Sensitivity and Specificity
D000970:Antineoplastic Agents; D002273:Carcinogens; D005456:Fluorescent Dyes; D015127:9,10-Dimethyl-1,2-benzanthracene; D017324:Hematoporphyrin Derivative