[Automated pre-column derivatization with o-phthaldialdehyde (OPA)> A new RP-HPLC method for the determination of biogenic amines in food].
A simple, selective and highly sensitive HPLC method for the routine determination of the biogenic amines in food is presented. Sample preparation is based on a rapid amine extraction using 10% trichloroacetic acid and a cation exchange column for extract purification. For the RP-HPLC analysis OPA/2-mercaptoethanol is used for the pre-column derivatisation, followed by fluorescence detection (Ex 345 nm, Em 440 nm). The effects of several factors are discussed. A separation of 15 biogenic amines is achieved within 70 min. The recoveries for histamine, tyramine, putrescine, cadaverine, tryptamine and beta-phenylethylamine are higher than 95%. The detection limits lie between 0.1-0.5 pMol/injection (20 microliters), depending on the amine and a good linearity is achieved in the range from 0.5-500 pMol (r > 0.99). The method has been applied for the determination of biogenic amines in swiss cheese, salami, milk, beer and wine, the repeatability is very good.
D000818:Animals; D001331:Automation / Q000379:methods; D001515:Beer / Q000032:analysis; D001679:Biogenic Amines / Q000032:analysis*; D002611:Cheese / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D005504:Food Analysis; D007202:Indicators and Reagents; D008461:Meat Products / Q000032:analysis; D008892:Milk / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods; D014920:Wine / Q000032:analysis; D009764:o-Phthalaldehyde
D001679:Biogenic Amines; D007202:Indicators and Reagents; D009764:o-Phthalaldehyde
Automatische Vorsäulenderivatisierung mit o-Phthaldialdehyd (OPA). Eine neue RP-HPLC-Methode für die Bestimmung von biogenen Aminen in Lebensmitteln.