Chlorsulfuron blocks 2,4-D-induced cell enlargement and NADH oxidase in excised sections of soybean hypocotyls.
Elongation of soybean hypocotyl sections induced by the auxin herbicide, 2,4-dichlorophenoxyacetic acid (2,4-D), was blocked by micromolar concentrations of the sulfonylurea herbicide chlorsulfuron (2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-trizin-2- yl)amino]carbonyl]benzenesulfonamide). The inhibition at equimolar concentrations of 2,4-D and chlorsulfuron was overcome by a 10- or 100-fold excess of 2,4-D. Stimulation by 2,4-D of an 2,4-D-responsive NADH-oxidase activity of the soybean plasma membrane also was blocked by the presence of concentrations of chlorsulfuron equimolar to the 2,4-D. Chlorsulfuron alone was largely without effect on either cell elongation or the auxin-stimulated NADH-oxidase activity over a range of chlorsulfuron concentrations. The results show a clear correlation between inhibition of auxin-stimulated cell elongation and the inhibition of an auxin-stimulated component of a plasma membrane NADH-oxidase activity.
D015084:2,4-Dichlorophenoxyacetic Acid / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D000095:Acetolactate Synthase / Q000037:antagonists & inhibitors; D002455:Cell Division / Q000187:drug effects*; D002462:Cell Membrane / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006540:Herbicides / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors*; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors*; D012997:Solvents / Q000494:pharmacology; D013025:Glycine max / Q000378:metabolism*; D013053:Spectrophotometry; D013449:Sulfonamides; D014227:Triazines / Q000494:pharmacology*; D014451:Ubiquinone / Q000494:pharmacology
D004791:Enzyme Inhibitors; D006540:Herbicides; D007210:Indoleacetic Acids; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D012997:Solvents; D013449:Sulfonamides; D014227:Triazines; D014451:Ubiquinone; D015084:2,4-Dichlorophenoxyacetic Acid; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D000095:Acetolactate Synthase; C037137:chlorsulfuron