[Influence of mannitol added to the nutrient solution on the mechanical performance and on the degree of myocardial edema of isolated hearts of rats].
OBJECTIVE
To analyse the influence of mannitol added to Krebs-Henseleit (KH) solution on the myocardium edema and myocardial function.


METHODS
Isolated rat heart under isovolumetric contractions studied according to Langendorff's technique were perfused with KH solution at constant flow during 90 min. The coronary perfusion pressure, diastolic and systolic pressures were recorded at every 15 min. At the end of the experiment, myocardium water content was measured in hearts perfused with KH solution (group I, n = 9) and in hearts perfused with KH solution plus 8mM mannitol (group II, n = 8). These results were compared to non-perfused control heart (n = 9).


RESULTS
Myocardial water content was statistically higher in group I (80.8 +/- 1.3%) compared to group II (78.1 +/- 0.7%) and control group (75.5 +/- 0.5%). Systolic arterial pressure was statistically higher in group I (86.2 +/- 11.5mmHg) compared to group II (72.7 +/- 21.1mmHg). There was no difference in the diastolic pressure between the two groups. Coronary perfusion pressure (Pp) increased progressively during the experiment in both groups. However, Pp was lower in group II than in group I.


CONCLUSIONS
Mannitol added to KH solution significantly attenuates the myocardium edema in the isolated perfused rat heart.
D000818:Animals; D001794:Blood Pressure; D001834:Body Water / Q000187:drug effects; D002314:Cardioplegic Solutions / Q000009:adverse effects*; D004234:Diuretics, Osmotic / Q000494:pharmacology*; D004489:Edema, Cardiac / Q000503:physiopathology*; D005947:Glucose / Q000008:administration & dosage; D066298:In Vitro Techniques; D008297:Male; D008353:Mannitol / Q000494:pharmacology*; D015999:Multivariate Analysis; D009200:Myocardial Contraction / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D014325:Tromethamine / Q000008:administration & dosage
D002314:Cardioplegic Solutions; D004234:Diuretics, Osmotic; C074097:Krebs-Henseleit solution; D014325:Tromethamine; D008353:Mannitol; D005947:Glucose
Influência do acréscimo de manitol à solução nutriente no desempenho mecânico e no grau de edema miocárdico de corações isolados de ratos.