Amino acid sequence and expression of the hepatic glycogen-binding (GL)-subunit of protein phosphatase-1.
A full-length cDNA encoding the putative hepatic glycogen-binding (GL) subunit of protein phosphatase-1 (PP1) was isolated from a rat liver library. The deduced amino acid sequence (284 residues, 32.6 kDa) was 23% identical (39% similar) to the N-terminal region of the glycogen-binding (GM) subunit of PP1 from striated muscle. The similarities between GM and GL were most striking between residues 63-86, 144-166 and 186-227 of human GM (approximately 40% identity), nearly all the identities with the putative yeast homologue GAC1 being located between 144-166 and 186-227. The cDNA was expressed in E. coli, and the expressed protein transformed the properties of PP1 to those characteristic of the hepatic glycogen-associated enzyme. These experiments establish that the cloned protein is GL.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D015723:Gene Library; D005982:Glutathione Transferase / Q000096:biosynthesis; D006801:Humans; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008112:Liver Glycogen / Q000378:metabolism*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology*; D016366:Open Reading Frames; D009928:Organ Specificity; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D010762:Phosphorylase a / Q000378:metabolism; D016133:Polymerase Chain Reaction; D054645:Protein Phosphatase 1; D011817:Rabbits; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D017931:DNA Primers; D008112:Liver Glycogen; D046911:Macromolecular Substances; D011993:Recombinant Fusion Proteins; D010762:Phosphorylase a; D005982:Glutathione Transferase; C026672:thiophosphorylase a; D010749:Phosphoprotein Phosphatases; C114052:Ppp1r3b protein, rat; D054645:Protein Phosphatase 1