title: Two genes encoding midgut-specific maltase-like polypeptides from Anopheles gambiae.
abstract: Full-length cDNA clones of two genes have been isolated from the African malaria vector mosquito, Anopheles gambiae. These genes, designated Agm1 and Agm2, encode maltase-like polypeptides of 498 and 599 residues, respectively. Deduced amino acid sequences contain a putative signal peptide sequence and four potential glycosylation sites. Agm1 and Agm2 show highest similarities to the Mal1 gene from Aedes aegypti and three clustered maltase genes from Drosophila melanogaster. Both genes are located at position 46D, in the terminal division of the left arm of the third chromosome. Agm2 has very strict tissue and temporal specificity, being expressed exclusively in the adult midgut. The specificity of Agm1 is similar but appears slightly broader; transcripts of this gene are detected at a low level in the pupae, and occasionally in the adult carcass after removal of the midgut.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000737:chemistry; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D017344:Genes, Insect; D007303:Insect Vectors / Q000201:enzymology / Q000235:genetics*; D008288:Malaria / Q000635:transmission; D008297:Male; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D014158:Transcription, Genetic; D000520:alpha-Glucosidases / Q000737:chemistry / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D012313:RNA; D000520:alpha-Glucosidases