Eimeria acervulina infection: evidence for the involvement of CD8+ T lymphocytes in sporozoite transport and host protection.
Host-parasite interactions during coccidial infection are complex, and host responses leading to parasite elimination are not well understood. Interactions between host cells and parasites were investigated using two-color immunofluorescent staining of duodenal tissue infected with Eimeria acervulina. Tissues were removed from TK strain chickens 24, 48, and 72 h after primary infection with 2.5 x 10(7) normal or irradiated oocysts and also following secondary infection with 2.5 x 10(7) normal oocysts. The tissues were embedded in freezing compound, frozen, sectioned, and fixed in cold acetone. Sections were stained with monoclonal antibodies to identify CD4+, CD8+, Ig+, and Bu1a+ lymphocytes, as well as macrophages and parasites. At 24 h after all infections, sporozoites were seen primarily in CD8+ lymphocytes and macrophages. Many CD8+ lymphocytes were seen at all times in infected tissues and frequently appeared to be in contact with infected epithelial cells. Sporozoites were occasionally seen in CD4+ lymphocytes but none were noted in Ig+ or Bu1a+ lymphocytes. Meront development was extensive after primary infection with normal oocysts, sparse after primary infection with irradiated oocysts, and moderate after secondary infection.
D000818:Animals; D000911:Antibodies, Monoclonal; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002645:Chickens; D003048:Coccidiosis / Q000276:immunology / Q000503:physiopathology / Q000662:veterinary*; D004539:Eimeria / Q000276:immunology*; D006790:Host-Parasite Interactions; D007700:Kinetics; D010784:Photomicrography; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology*; D013997:Time Factors
D000911:Antibodies, Monoclonal