The purification and protective capacity of Bordetella pertussis outer membrane proteins.
The whole cell vaccine (WCV) of Bordetella pertussis is protective in the intracerebral (i.c.) mouse protection assay. We found a correlation between the i.c. mouse protection assay potency and the presence of the virulence-associated outer membrane proteins (OMPs) in outer membrane complexes (OMC). The virulence-associated 92, 32 and 30 kDa OMPs were purified and the N-terminal amino acid sequences were determined. The N-terminal amino acid sequences of the 30 and 32 kDa OMPs show homology with the C-terminal fragment of the P.93 precursor of the 69 kDa OMP (pertactin). The purified 32 kDa OMP was protective in the i.c. test when presented as mixed protein-detergent micelles. The 92 kDa OMP became a protective antigen when nonprotective levels of pertussis toxin were added. We found a correlation between the i.c. mouse protection value and the 92 kDal38 kDa (porin) ratio in OMC preparations.
D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification*; D001428:Bacterial Vaccines / Q000737:chemistry / Q000276:immunology* / Q000627:therapeutic use; D001885:Bordetella Infections / Q000382:microbiology / Q000517:prevention & control*; D001886:Bordetella pertussis / Q000276:immunology* / Q000472:pathogenicity; D004339:Drug Compounding; D005260:Female; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D008823:Micelles; D008969:Molecular Sequence Data
D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D008823:Micelles