title: Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C.
abstract: The gene coding for opine dehydrogenase from Arthrobacter sp. strain 1C was cloned onto plasmid pBluescript KS(-), and the nucleotide sequence of the 1,077-bp open reading frame consisting of 359 codons was identified as the odh gene. Transformed Escherichia coli cells overproduced NAD+-dependent opine dehydrogenase under control of the promoter of the lac gene on pBluescript KS(-).
mesh_terms: D016960:Agrobacterium tumefaciens / Q000201:enzymology / Q000235:genetics; D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003605:D-Amino-Acid Oxidase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007763:Lac Operon; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D003605:D-Amino-Acid Oxidase; D000587:Oxidoreductases Acting on CH-NH Group Donors; C060511:opine dehydrogenase, NAD+; C021523:D-lysopine dehydrogenase