High-performance liquid chromatography of amino acids after derivatization with 9-isothiocyanatoacridine.
This study describes a reproducible method for the routine determination of amino acids in protein hydrolysates using 9-isothiocyanatoacridine as an HPLC precolumn derivatization reagent. Derivatization is carried out by adding the isothiocyanate dissolved in dry acetonitrile to a buffered amino acid solution without the following extraction of the excess reagent. The resulting N-(9-acridinylthiocarbamoyl)amino acids were separated by one single isocratic HPLC analysis with the UV detection at 280 nm.
D000166:Acridines; D000596:Amino Acids / Q000032:analysis* / Q000302:isolation & purification; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007202:Indicators and Reagents; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000166:Acridines; D000596:Amino Acids; D007202:Indicators and Reagents; C005921:isothiocyanatoacridine