title: Degradation of purines: only ureidoglycollate lyase out of four allantoin-degrading enzymes is present in mammals.
abstract: It is generally accepted that all of the allantoin-degrading enzymes (allantoinase, allantoicase, ureidoglycollate lyase and urease), used in purine degradation, were lost during mammalian evolution. However, surprisingly, ureidoglycollate lyase has been found in a mammalian tissue. Ureidoglycollate lyase was purified to homogeneity and characterized from rat-liver mitochondria. The apparent Km (17 mM) of the rat enzyme for ureidoglycollate was much higher than that (0.33 mM) of fish-liver ureidoglycollate lyase. The rat-liver enzyme differed from the fish-liver enzyme in enzymic, physical and immunological properties.
mesh_terms: D000481:Allantoin / Q000378:metabolism*; D019760:Amidine-Lyases; D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D005075:Biological Evolution; D002458:Cell Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D006038:Glyoxylates / Q000378:metabolism; D015151:Immunoblotting; D008190:Lyases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D008322:Mammals / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000201:enzymology*; D011687:Purines / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D014508:Urea / Q000378:metabolism; D014510:Urease / Q000378:metabolism; D014512:Ureohydrolases / Q000378:metabolism
chemical_list: D006038:Glyoxylates; D011687:Purines; D000481:Allantoin; D014508:Urea; D000581:Amidohydrolases; D014510:Urease; C019659:allantoinase; D014512:Ureohydrolases; C019658:allantoicase; D008190:Lyases; D019760:Amidine-Lyases; C033757:ureidoglycollate lyase; C031150:glyoxylic acid