title: Distributions of nitropyrenes and mutagenicity in airborne particulates collected with an Andersen sampler.
abstract: Distributions of 1,3-dinitropyrene (1,3-DNP), 1,6-DNP, 1,8-DNP, 1-nitropyrene (1-NP) and mutagenicity in airborne particulates collected in downtown Kanazawa, Japan with an Andersen high-volume air sampler were examined. Mutagenicities of benzene-ethanol extract from particulates were determined by the Ames test using S. typhimurium strains without S9 mix, while concentrations of DNPs and 1-NP were determined by high-performance liquid chromatography (HPLC) using chemiluminescence detection. In the finest particulate fraction (smaller than 1.1 microns), 68% and 75% of the total mutagenicities were observed in TA98 and YG1024 strains, respectively. In the same fraction, 65-82% of three DNPs as well as 84% of 1-NP were observed. Mutagenic contributions of 1,3-DNP, 1,6-DNP, 1,8-DNP and 1-NP in the extract were respectively 0.6, 1.2, 1.8 and 1.6% in the TA98 strain, and 2.5, 5, 9 and 2.1% in the YG1024 strain.
mesh_terms: D000397:Air Pollution / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D009152:Mutagenicity Tests; D009153:Mutagens / Q000032:analysis*; D011721:Pyrenes / Q000032:analysis* / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects
chemical_list: D009153:Mutagens; D011721:Pyrenes; C033310:1,8-dinitropyrene; C033309:1,6-dinitropyrene; C037413:1,3-dinitropyrene; C032668:1-nitropyrene