Pattern of cytokine production by T cells from adolescents with asthma in remission, after stimulation with Dermatophagoides farinae antigen.
Children with asthma usually become asymptomatic by the time they reach age 20 y. To clarify the immunologic mechanisms responsible for this phenomenon, we studied patients in remission and others who still had frequent asthma attacks. Patients were grouped by clinical status, and three variables were measured: serum levels of IgE, production of IL4 and interferon (IFN)-gamma, and the activation of T cells induced by Dermatophagoides farinae (Df) antigen. Df-induced activation of T cells (as measured by antigen-induced IL2 responsiveness) or IL2 synthesis itself was induced in patients with active asthma but not in normal subjects. These responses were much weaker in patients in remission. When stimulated by Df antigen in vitro, lymphocytes from patients with active asthma produced much more IL4 than did the cells from normal subjects, and cells from patients in remission produced only a small amount. In contrast, under similar conditions lymphocytes from patients with active asthma produced less IFN-gamma than did the cells from normal subjects. Production of IFN-gamma stimulated by Df antigen was high in patients in remission but not in normal subjects. Thus, upregulated IFN-gamma production after exposure to Df antigen might reduce IL4 secretion, which would suppress IgE production and would improve clinical status. Df antigen may suppress Df-induced allergic responses in patients with asthma in remission.
D000293:Adolescent; D000328:Adult; D000818:Animals; D000917:Antibody Formation; D001249:Asthma / Q000097:blood* / Q000276:immunology; D016022:Case-Control Studies; D002648:Child; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood; D004391:Dust; D000939:Epitopes; D005260:Female; D006801:Humans; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008297:Male; D008925:Mites / Q000276:immunology*; D012075:Remission, Spontaneous; D013601:T-Lymphocytes / Q000378:metabolism*
D016207:Cytokines; D004391:Dust; D000939:Epitopes; D007376:Interleukin-2; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma