Epstein-Barr virus induction of recombinase-activating genes RAG1 and RAG2.
In experimental B-cell infections, Epstein-Barr virus induced sustained expression of V(D)J recombinase-activating genes RAG1 and RAG2, whose aberrant activity has been implicated in chromosomal translocations in B-cell neoplasms. In cell lines in which RAG1 and RAG2 were detected, virus integrated into cellular DNA rather than assumed the configuration of extrachromosomal episomes. Expression of the Epstein-Barr virus nuclear antigen 1 in transient transfection assays was sufficient to induce both recombinase-activating genes.
D000956:Antigens, Viral / Q000378:metabolism; D001483:Base Sequence; D002051:Burkitt Lymphoma; D002460:Cell Line; D004254:DNA Nucleotidyltransferases / Q000096:biosynthesis*; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000378:metabolism; D004789:Enzyme Activation; D019309:Epstein-Barr Virus Nuclear Antigens; D015971:Gene Expression Regulation, Enzymologic; D015967:Gene Expression Regulation, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D015534:Trans-Activators / Q000378:metabolism; D014407:Tumor Cells, Cultured; D045567:VDJ Recombinases; D016662:Virus Integration
D000956:Antigens, Viral; D017931:DNA Primers; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D015534:Trans-Activators; D004254:DNA Nucleotidyltransferases; D045567:VDJ Recombinases