Kinetic potentiometric determination of creatinine in serum with a picrate ion-selective membrane electrode.
We describe a new kinetic method for potentiometric determination of creatinine in serum, based on the creatinine--picrate reaction in alkaline medium (Jaffé reaction). The reaction is monitored with a picrate-selective electrode, and the increase in electrode potential during 270 s is measured and related directly to the creatinine concentration. Small cation-exchange columns are used to separate creatinine from interfering substances. Analytical recovery of creatinine added to serum was 100.7%. Results for a series of samples compared well with results obtained with a spectrophotometric method (r = 0.994).
D003404:Creatinine / Q000097:blood*; D004566:Electrodes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010851:Picrates; D011199:Potentiometry / Q000295:instrumentation
D010851:Picrates; D003404:Creatinine