Screening for apoptosis inducers in microbial products and induction of apoptosis by cytostatin.
We have employed the DNA-methylgreen binding assay as a primary screening method for identifying apoptosis inducers in microbial products. Capsimycin, toyocamycin and cytostatin affect the binding of methylgreen to DNA of FS3 cells in this test system. The effect of cytostatin on apoptosis induction was confirmed by means of the ELISA system.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005354:Fibrosarcoma; D008739:Methyl Green; D051379:Mice; D009930:Organic Chemicals; D010755:Organophosphates / Q000494:pharmacology*; D011753:Pyrones / Q000494:pharmacology*; D013053:Spectrophotometry; D014127:Toyocamycin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D000935:Antifungal Agents; D004273:DNA, Neoplasm; D009930:Organic Chemicals; D010755:Organophosphates; D011753:Pyrones; C088526:cytostatin; C019287:capsimycin; D008739:Methyl Green; D014127:Toyocamycin