Modulation of expression of genes encoding nuclear proteins following exposure to JANUS neutrons or gamma-rays.
Previous work has shown that exposure of cells to ionizing radiations causes modulation of a variety of genes, including those encoding c-fos, interleukin-1, tumor necrosis factor, cytoskeletal elements, and many more. The experiments reported herein were designed to examine the effects of either JANUS neutron or gamma-ray exposure on expression of genes encoding nucleus-associated proteins (H4-histone, c-jun, c-myc, Rb, and p53). Cycling Syrian hamster embryo cells were irradiated with varying doses and dose rates of either JANUS fission-spectrum neutrons or gamma-rays; after incubation of the cell cultures for 1 h following radiation exposure, mRNA was harvested and analyzed by Northern blot. Results revealed induction of transcripts for c-jun, H4-histone, and (to a lesser extent) Rb following gamma-ray but not following neutron exposure. Interestingly, expression of c-myc was repressed following gamma-ray but not following neutron exposure. Radiations at different doses and dose rates were compared for each of the genes studied.
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D005720:Gamma Rays; D005786:Gene Expression Regulation / Q000528:radiation effects*; D016161:Genes, Retinoblastoma; D016758:Genes, jun; D016259:Genes, myc; D016158:Genes, p53; D006657:Histones / Q000235:genetics; D008647:Mesocricetus; D009502:Neutrons; D009687:Nuclear Proteins / Q000235:genetics*
D006657:Histones; D009687:Nuclear Proteins