Mitogenic response to TGF-beta in 3T3-F442A cells.
In 3T3-F442A cells, TGF-beta caused cellular proliferation in a time and dose-dependent manner. TGF-beta induced cyclin D1 and cdk2 proteins in 3T3-F442A cells. The mitogenic effect of TGF-beta was specific in nature. The antimitogenic agent, hGH, inhibited the mitogenic effect of TGF-beta and was associated with inhibition of cyclin D1 expression. The protein kinase c inhibitor, staurosporine, inhibited the mitogenic effect of TGF-beta. Taken together, these results suggest that TGF-beta affects expression levels of cell cycle-regulated proteins and its mitogenic effect is mediated through protein kinase C in 3T3-F442A cells.
D016475:3T3 Cells; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D002455:Cell Division; D019938:Cyclin D1; D016213:Cyclins / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D013006:Growth Hormone / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D015513:Oncogene Proteins / Q000037:antagonists & inhibitors; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism; D019311:Staurosporine; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D000470:Alkaloids; D016213:Cyclins; D015513:Oncogene Proteins; D016212:Transforming Growth Factor beta; D019938:Cyclin D1; D013006:Growth Hormone; D011493:Protein Kinase C; D019311:Staurosporine