Members of a family of proteins (the RD family) detected by a U1 70K monoclonal antibody are present in spliceosomal complexes.
We have characterized a monoclonal antibody (mAb) to the U1 snRNP component U1 70K. We find that this antibody recognizes several proteins, in addition to U1 70K, in purified spliceosomal complexes and in total HeLa cell nuclear extract preparations. The novel mAb U1 70K antigens can also be specifically immunoprecipitated by the antibody. Similarly to U1 70K, many of the mAb U1 70K antigens can be phosphorylated by a co-purifying kinase activity. The epitope recognized by mAb U1 70K was previously shown to be a repeating arginine/aspartate (RD) dipeptide. Thus we have designated the novel mAb U1 70K antigens the RD family. Comparison of mAb U1 70K with a recently characterized antibody, mAb 16H3, whose epitope is a repeating R/D or R/E motif, showed that a large subset of the antigens are common. In contrast, most of the mAb U1 70K antigens are distinct from the proteins detected by mAb 104, an antibody to the SR family of splicing factors.
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002457:Cell Extracts; D002467:Cell Nucleus / Q000378:metabolism; D004151:Dipeptides; D000939:Epitopes / Q000032:analysis; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016601:RNA-Binding Proteins / Q000032:analysis* / Q000737:chemistry; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000032:analysis*; D017381:Spliceosomes / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D002457:Cell Extracts; D004151:Dipeptides; D000939:Epitopes; D016601:RNA-Binding Proteins; D017412:Ribonucleoprotein, U1 Small Nuclear; C490688:SNRNP70 protein, human