Stability against temperature and external agents of vesicles composed of archael bolaform lipids and egg PC.
The bolaform lipid PLE extracted from the thermophilic archaeon Sulfolobus solfataricus and its mixtures with egg phosphatidyl-choline (egg PC) have been used to prepare sonicated vesicles. The leakage of entrapped calcein was continuously monitored by fluorescence dequenching. The half times of leakage have been used to compare vesicle stability under different conditions of temperature, lipid composition and presence of destabilizing agents like Ca2+ ions and poly(ethylene glycol) (PEG). It has been found that leakage is primarily modulated by the monopolar/bipolar lipid ratio. In particular, the half time of leakage for vesicles formed from a mixture of the polar lipid extract (PLE) and egg PC is characterized by a maximum at about 1:2 molar ratio. The free energy of mixing has been evaluated from pressure-area isotherms on monolayers at the air/water interface. The results indicate a non monotonous behaviour of the excess free energy of mixing as a function of the molar ratio and the occurrence of a minimum at a fixed molar ratio. The possible formation of a complex is discussed and compared with previous calorimetric measurements on similar compounds.
D002118:Calcium / Q000494:pharmacology; D005452:Fluoresceins / Q000378:metabolism; D007202:Indicators and Reagents / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000378:metabolism*; D015394:Molecular Structure; D010539:Permeability; D010713:Phosphatidylcholines / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D016944:Sulfolobus / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
D005452:Fluoresceins; D007202:Indicators and Reagents; D008055:Lipids; D008081:Liposomes; D010713:Phosphatidylcholines; D011092:Polyethylene Glycols; D002118:Calcium; C007740:fluorexon