Detection of beta-2 transferrin in otorrhea and rhinorrhea in a routine clinical laboratory setting.
A simple, straightforward, and rapid method for the detection of beta-2 transferrin in otorrhea and rhinorrhea that can be used in a routine clinical laboratory is described. The beta-2 transferrin was detected by agarose gel electrophoresis of the fluid on Beckman Paragon equipment, followed by pressure transfer to a nitrocellulose membrane and then incubation with enzyme-labeled antitransferrin antibody and substrate. The procedure was fast (3.5 h) and sensitive (detected as little as 1 microgram/ml) and required only 3 microliters of fluid. Beta-2 transferrin was detected in cerebrospinal fluid diluted up to eightfold. No special training or expertise was needed, and all equipment and procedures used are commonly available in a routine clinical laboratory.
D002558:Cerebrospinal Fluid Otorrhea / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D002559:Cerebrospinal Fluid Rhinorrhea / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D002624:Chemistry, Clinical / Q000379:methods; D004586:Electrophoresis / Q000379:methods; D006801:Humans; D015151:Immunoblotting / Q000379:methods; D012680:Sensitivity and Specificity; D014168:Transferrin / Q000134:cerebrospinal fluid*
D014168:Transferrin