B-50 (GAP-43) in the rat spinal cord caudal to hemisection: lack of intraspinal sprouting by dorsal root axons.
The controversial hypothesis that intraspinal sprouting by dorsal root axons promotes reinnervation of partially denervated neurons caudal to a low thoracic cord hemisection was re-investigated in rats using quantitative immunohistochemical analysis of the neural specific growth-associated protein B-50 (GAP-43) at postoperative survival times of 3, 10, 21, 42, and 90 days. The lack of increase in B-50-immunoreactivity in all segments below the hemisection at all survival times does not support the concept of intraspinal sprouting following the removal of supraspinal descending pathways.
D000818:Animals; D001369:Axons / Q000378:metabolism; D005260:Female; D019922:GAP-43 Protein; D005727:Ganglia, Spinal / Q000276:immunology / Q000378:metabolism*; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000378:metabolism*; D009410:Nerve Degeneration; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000378:metabolism*; D009473:Neuronal Plasticity / Q000502:physiology; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000378:metabolism*; D017966:Pyramidal Cells / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D013116:Spinal Cord / Q000276:immunology / Q000378:metabolism*; D013119:Spinal Cord Injuries; D013997:Time Factors
D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins