Automated DNA purification and amplification from blood-stained cards using a robotic workstation.
A new method for the purification of DNA on blood-stained cards was developed. This method was implemented into a high-throughput automated system using a Biomek 1000 robotic workstation. In addition, the processes of DNA purification and amplification were coupled into a completely automated and uninterrupted prototype system, and the resultant PCR products generated by this system were subjected to automated desalting for capillary electrophoresis analysis.
D001322:Autoanalysis; D001483:Base Sequence; D002197:Capillary Action; D002803:Chondro-4-Sulfatase / Q000235:genetics; D004247:DNA / Q000097:blood* / Q000737:chemistry; D004586:Electrophoresis; D006683:HLA-DQ Antigens / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D004247:DNA; D002803:Chondro-4-Sulfatase