title: DAN gene product has an affinity for Ni2+.
abstract: Differential screening-selected gene aberrative in neuroblastoma (DAN) encodes a protein which possesses metal binding motifs. Glutathione S-transferase DAN fusion protein had an ability to bind to Ni(2+)-immobilized affinity resin. Truncation of the C-terminal region including a (HX)n repeat of DAN caused a loss of binding ability to the affinity resin, suggesting that this region is essential for Ni(2+)-binding. DAN produced in cultured rat cells also had an affinity for Ni2+. Cross-linking experiments demonstrated that the C-terminal region might function as a protein-protein interacting domain.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D003432:Cross-Linking Reagents; D016147:Genes, Tumor Suppressor; D005982:Glutathione Transferase / Q000096:biosynthesis; D008322:Mammals; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009419:Nerve Tissue Proteins; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism; D009532:Nickel / Q000378:metabolism*; D011485:Protein Binding; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D017384:Sequence Deletion
chemical_list: D003432:Cross-Linking Reagents; C085763:Nbl1 protein, rat; D009419:Nerve Tissue Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D009532:Nickel; D005982:Glutathione Transferase