The effect of endotoxin on alcohol dehydrogenase (ADH) activity in the serum of ethanol intoxicated rats.
Serum alcohol dehydrogenase activity at different pH-values (8.8, 9.6 and 10.4) was investigated in induced endotoxemia (3 mg/kg) after chronic alcohol consumption in rats (60 days, 11.5 mg/kg, per day). It has been shown that ADH activity is higher after the single than after the triple injection of endotoxin given the healthy rats. Such a difference was not observed in the ethanol-intoxicated rats. The single dose of endotoxin did not maintain the increased ADH activity after alcohol administration, however a triple injection of endotoxin maintained the activity but did not increase. This results show, that the highest ADH activity in all tested rats was observed at pH 9.6 and that chronic ethanol consumption did not cause an increase of the toxic effects of endotoxin on liver cells, measured by serum alcohol dehydrogenase activity.
D000410:Alanine Transaminase / Q000378:metabolism; D000426:Alcohol Dehydrogenase / Q000097:blood*; D000435:Alcoholic Intoxication / Q000201:enzymology*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D004731:Endotoxins / Q000633:toxicity*; D004926:Escherichia coli; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric
D004731:Endotoxins; D000426:Alcohol Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase