Calcium signals in olfactory neurons.
Laser scanning confocal microscopy in combination with the fluorescent calcium indicators Fluo-3 and Fura-Red was employed to estimate the intracellular concentration of free calcium ions in individual olfactory receptor neurons and to monitor temporal and spatial changes in the Ca(2+)-level upon stimulation. The chemosensory cells responded to odorants with a significant increase in the calcium concentration, preferentially in the dendritic knob. Applying various stimulation paradigma, it was found that in a population of isolated cells, subsets of receptor neurons display distinct patterns of responsiveness.
D000814:Aniline Compounds; D000818:Animals; D001572:Benzofurans; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes; D007093:Imidazoles; D066298:In Vitro Techniques; D007834:Lasers; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009812:Odorants; D018034:Olfactory Receptor Neurons / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction; D014966:Xanthenes
D000814:Aniline Compounds; D001572:Benzofurans; D005456:Fluorescent Dyes; D007093:Imidazoles; D014966:Xanthenes; C082877:fura red; C059715:Fluo-3; D002118:Calcium