Gut inflammation in children with cystic fibrosis on high-dose enzyme supplements.
We used a whole-gut perfusion technique to study subclinical gut inflammation in children with cystic fibrosis (18 elective tests, three lavages to treat distal intestinal obstruction syndrome); and in 12 control children with constipation or pre-colonoscopy. We assayed for haemoglobin, IgG, albumin, alpha-1-antitrypsin, granulocyte elastase, interleukin-1 beta (IL-1 beta) and IL-8 concentrations in whole-gut lavage fluid. Results for two children with distal intestinal obstruction syndrome, the only children in the series taking Nutrizym 22, were strikingly abnormal. This new test has revealed subclinical gut mucosal inflammation in a minority of CF children, for which distal intestinal obstruction syndrome, Nutrizym 22 treatment, or both, may be risk factors.
D000293:Adolescent; D000418:Albumins / Q000032:analysis; D000681:Amylases / Q000008:administration & dosage / Q000009:adverse effects*; D001963:Bromelains / Q000008:administration & dosage / Q000009:adverse effects*; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003092:Colitis / Q000139:chemically induced* / Q000378:metabolism; D003113:Colonoscopy; D003248:Constipation / Q000378:metabolism; D003550:Cystic Fibrosis / Q000188:drug therapy*; D004338:Drug Combinations; D005260:Female; D005765:Gastrointestinal Agents / Q000008:administration & dosage / Q000009:adverse effects*; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007375:Interleukin-1 / Q000032:analysis; D016209:Interleukin-8 / Q000032:analysis; D007415:Intestinal Obstruction / Q000139:chemically induced / Q000378:metabolism; D019272:Leukocyte Elastase; D008049:Lipase / Q000008:administration & dosage / Q000009:adverse effects*; D008297:Male; D010196:Pancreatic Elastase / Q000032:analysis; D010184:Pancreatic Extracts / Q000009:adverse effects*; D010194:Pancreatin / Q000008:administration & dosage / Q000009:adverse effects*; D020799:Pancrelipase; D012307:Risk Factors; D013577:Syndrome; D007507:Therapeutic Irrigation; D014357:Trypsin / Q000008:administration & dosage / Q000009:adverse effects*; D000515:alpha 1-Antitrypsin / Q000032:analysis
D000418:Albumins; D004338:Drug Combinations; D005765:Gastrointestinal Agents; D006454:Hemoglobins; D007074:Immunoglobulin G; D007375:Interleukin-1; D016209:Interleukin-8; C002762:Nutrizym; D010184:Pancreatic Extracts; D000515:alpha 1-Antitrypsin; D020799:Pancrelipase; D010194:Pancreatin; D001963:Bromelains; D008049:Lipase; D000681:Amylases; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase; D014357:Trypsin