A short-chain dehydrogenase gene from Pichia stipitis having D-arabinitol dehydrogenase activity.
An NAD(+)-dependent D-arabinitol dehydrogenase (polyol dehydrogenase) gene was isolated from Pichia stipitis CBS 6054 and cloned in Saccharomyces cerevisiae. The gene was isolated by screening of a lambda-cDNA library with a zymogram technique. D-Arabinitol, xylitol, D-glucitol and galactitol are substrates for the recombinant protein. With D-arabinitol as substrate the reaction product is D-ribulose. The molecular weight of the native tetramer enzyme is 110,000 Da and the monomer is 30,000 Da. The amino acid sequence is homologous to the short-chain dehydrogenase family. It is 85.5% identical to a D-arabinitol dehydrogenase from Candida albicans. The gene in P. stipitis was induced by D-arabinitol and P. stipitis was able to grow on D-arabinitol. The physiological role of D-arabinitol metabolism is discussed.
D000595:Amino Acid Sequence; D001089:Arabinose / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D010843:Pichia / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics* / Q000378:metabolism; D013402:Sugar Alcohols / Q000378:metabolism
D017931:DNA Primers; D018076:DNA, Complementary; D004271:DNA, Fungal; D013402:Sugar Alcohols; D001089:Arabinose; D013401:Sugar Alcohol Dehydrogenases; C022751:D-arabinitol dehydrogenase; C014999:arabitol