Effect of two new polysaccharides on growth, agglomeration and zeta potential of calcium phosphate crystals.
OBJECTIVE
To study the effect of semisynthetic sulphated polysaccharides in the different calcium phosphate crystallization processes in vitro.


METHODS
Crystallization of hydroxyapatite (HAP) and brushite (DCPD) in the presence and absence of 2 new semisynthetic sulphated polysaccharides (G871, G872) were defined by a constant composition technique, particle size analysis and zeta potential measurement.


RESULTS
These polysaccharides demonstrated strong inhibitory effect on HAP and DCPD crystal growth and agglomeration. The increase of negative zeta potential values after addition of polysaccharides suggests the binding of these polysaccharides to HAP and DCPD crystals.


CONCLUSIONS
We conclude that both G871 and G872 could be of potential use for calcium phosphate urolithiasis prevention in addition to their use for calcium oxalate.
D002130:Calcium Phosphates / Q000737:chemistry*; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D010426:Pentosan Sulfuric Polyester / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D014545:Urinary Calculi / Q000737:chemistry / Q000517:prevention & control
D002130:Calcium Phosphates; D011134:Polysaccharides; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D010426:Pentosan Sulfuric Polyester; C485838:calcium phosphate, monobasic, anhydrous; D017886:Durapatite; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous; C494366:calcium phosphate, dibasic, dihydrate