A new microelectrophoretic procedure for delivery of horseradish peroxidase.
A new procedure for microelectrophoretic delivery of horseradish peroxidase (HRP) is described. A dilute solution (1.5--3% w/v) of fluorescein isothiocyanate-conjugated HRP is deposited according to a standard microelectrophoretic method and the tissue reacted according to a sensitive histochemical procedure. A major advantage of this method is the reduced secondary diffusion of the enzyme present at the injection site.
D000818:Animals; D001931:Brain Mapping / Q000379:methods*; D004586:Electrophoresis; D005452:Fluoresceins; D006735:Horseradish Peroxidase / Q000008:administration & dosage*; D007031:Hypothalamus / Q000033:anatomy & histology; D010544:Peroxidases / Q000008:administration & dosage*; D051381:Rats
D005452:Fluoresceins; D006735:Horseradish Peroxidase; D010544:Peroxidases