Measurement of rabies virus N protein in rabies vaccines.
In this study, we designed a capture ELISA using a monoclonal antibody specific for the N protein, the major protein of the rabies virus nucleocapsid, to measure the N protein content in rabies vaccines. Free N protein content was compared in the two types of rabies vaccine currently used in humans: suckling mouse brain (SMB) vaccine prepared from rabies virus-infected brain tissue, and tissue culture (TC) vaccine prepared from supernatants of rabies virus-infected cells. It was found that SMB vaccines contained considerably higher amounts of N protein than most of the TC vaccines. Possible implications concerning the efficacy of rabies vaccines are discussed.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral; D002213:Capsid / Q000032:analysis* / Q000276:immunology; D002460:Cell Line; D046508:Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology; D006801:Humans; D051379:Mice; D011819:Rabies Vaccines / Q000737:chemistry* / Q000145:classification; D011820:Rabies virus / Q000737:chemistry*; D014758:Viral Core Proteins / Q000032:analysis* / Q000276:immunology; D014759:Viral Envelope Proteins / Q000032:analysis / Q000276:immunology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D006023:Glycoproteins; D011819:Rabies Vaccines; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins; C064951:glycoprotein G, Rabies virus