title: Visualization of the transport pathways of low density lipoproteins across the endothelial cells in the branched regions of rat arteries.
abstract: The transport pathways of low density lipoproteins (LDL) across the endothelium at the branched and unbranched regions of the artery were studied in high cholesterol diet-fed rats. Rat tissues were analyzed by perfusing in situ human or rat LDL labeled with colloidal gold or fluorescein 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). Results indicated that more LDL-DiI accumulated in the branched regions than in the unbranched regions of the artery. LDL-gold conjugates were observed in the plasmalemmal vesicles, multivesicular bodies and in the subendothelial space in both the branched and the unbranched regions of the arteries. Quantitative study revealed that the volume densities of plasmalemmal vesicles which contained the LDL-gold particles in the branched regions of the aortic arch were significantly (P < 0.05) higher than the density value in the unbranched regions of the thoracic aorta, whereas there was no marked difference in the density value of multivesicular bodies between these two regions. The open junctions with gap widths of 30-450 nm between adjacent endothelial cells were only observed in the branched regions of the aortic arch, whereas no open junctions were present in the unbranched regions of the thoracic aorta. Moreover, the LDL-gold conjugates were present within most of these open junctions. In all specimens examined, no gold particles were found in the normal intercellular channels (i.e., 25 nm and less) of both regions. These results indicated that the major visible routes for transport of LDL across the endothelium in the branched regions of the arteries are open junctions as well as plasmalemmal vesicles. The region-associated permeability changes of LDL might account for the incidence of atherosclerosis in the branched areas of arteries.
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism* / Q000473:pathology; D001692:Biological Transport; D001835:Body Weight / Q000187:drug effects; D002232:Carbocyanines; D002791:Cholesterol, Dietary / Q000633:toxicity; D004036:Diet, Atherogenic; D004730:Endothelium, Vascular / Q000378:metabolism*; D005456:Fluorescent Dyes; D006801:Humans; D006937:Hypercholesterolemia / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D007365:Intercellular Junctions / Q000648:ultrastructure; D008077:Lipoproteins, LDL / Q000378:metabolism* / Q000493:pharmacokinetics; D008297:Male; D008856:Microscopy, Fluorescence; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002232:Carbocyanines; D002791:Cholesterol, Dietary; D005456:Fluorescent Dyes; D008077:Lipoproteins, LDL; C024286:3,3'-dioctadecylindocarbocyanine