[Determination of Escherichia coli Shiga-like toxins by means of the MTT bioassay].
Tissue culture cells' metabolism and viability are measured by the mitochondrial reduction rate of a yellow tetrazolium salt (MTT) to blue formazan crystals in the MTT-bioassay. Thus the MTT-bioassay is a standardizable and reproducible bioassay for measuring cytotoxicity or cytostimulation. It is shown that the MTT-bioassay is also very suitable for determining bacterial cytotoxins using Escherichia coli's Shiga-like toxins as example. 177 strains of E. coli, isolated from carcasses and organs of cattle, are classified biochemically and tested for cytotoxin production by means of the MTT-bioassay. One of these strains is recognized as producer of Shiga-like toxin 2. 4 Enterohemolysin-producing strains of E. coli are cultivated from a feces sample of a diarrhoeic nubian ibex and identified as Shiga-like toxin 1 producers by help of the MTT-bioassay.
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis* / Q000633:toxicity; D001681:Biological Assay; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003603:Cytotoxins / Q000032:analysis* / Q000633:toxicity; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D005562:Formazans; D008460:Meat / Q000382:microbiology*; D012418:Ruminants; D022641:Shiga Toxin 2; D013778:Tetrazolium Salts
D001427:Bacterial Toxins; D003603:Cytotoxins; D005562:Formazans; D022641:Shiga Toxin 2; D013778:Tetrazolium Salts; C079782:MTT formazan
Zum Nachweis der Shiga-like Toxine von Escherichia coli mit Hilfe des MTT-Zellkulturtests.