In-vivo genotoxicity of the alkaloid drug pilocarpine nitrate in bone marrow cells and male germ cells of mice.
Pilocarpine nitrate, an alkaloid drug of plant origin induces spindle disfunction in bone marrow cells of mice. Further studies were carried out to investigate its mutagenic effects in somatic and germ cells of mice by assessing chromosome aberrations at mitotic metaphase and as micronuclei in bone marrow cells and sperm-shape abnormality in cauda epididymides. The dose and time yield effects of the drug were investigated. The statistically significant results that were obtained for both chromosomal aberrations and micronucleus test but not for the sperm-shape abnormality test, indicated the genotoxicity of this compound in somatic cells but not in germ cells.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects*; D001854:Bone Marrow Cells; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D008916:Miotics; D009153:Mutagens / Q000633:toxicity*; D010277:Parasympathomimetics / Q000633:toxicity*; D010862:Pilocarpine / Q000633:toxicity*; D013094:Spermatozoa / Q000166:cytology / Q000187:drug effects*
D008916:Miotics; D009153:Mutagens; D010277:Parasympathomimetics; D010862:Pilocarpine