A strategy for quickly identifying all unique two-hybrid or library plasmids within a pool of yeast transformants.
D049069:ATP-Dependent Proteases; D003001:Cloning, Molecular / Q000379:methods*; D018628:Gene Dosage; D005800:Genes, Fungal / Q000235:genetics; D016153:Genes, Suppressor / Q000235:genetics; D015698:Genomic Library; D020662:Guanine Nucleotide Exchange Factors; D007930:Leucine; D024101:Mitochondrial Proteins; D015336:Molecular Probe Techniques; D009693:Nucleic Acid Hybridization; D010957:Plasmids / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics*; D029702:Schizosaccharomyces pombe Proteins; D012697:Serine Endopeptidases / Q000235:genetics
D020662:Guanine Nucleotide Exchange Factors; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C490057:pim1 protein, S pombe; D049069:ATP-Dependent Proteases; C085155:PIM1 protein, S cerevisiae; D012697:Serine Endopeptidases; D007930:Leucine