Didemnin B induces cell death by apoptosis: the fastest induction of apoptosis ever described.
Didemnin B, a cyclic N-methylated peptolide induces apoptosis in human HL-60 cells. When incubated with 1 microM didemnin B, unsynchronized HL-60 cultures undergo apoptosis to 100% within 140 minutes. Apoptosis has been assessed by the typical apoptotic morphology, the presence of double-stranded DNA fragments within the cytosol and the generation of DNA ladders. None of these characteristics of apoptosis are seen when HL-60 cells are pretreated with 1mM Zn2+ immediately before treatment with didemnin B.
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000032:analysis / Q000187:drug effects; D047630:Depsipeptides; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D007700:Kinetics; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000494:pharmacology*; D013997:Time Factors; D017967:Zinc Compounds / Q000494:pharmacology
D000970:Antineoplastic Agents; D002712:Chlorides; D004273:DNA, Neoplasm; D047630:Depsipeptides; D010456:Peptides, Cyclic; D017967:Zinc Compounds; D000431:Ethanol; C030051:didemnins; C016837:zinc chloride