Mouse rhodanese gene (Tst): cDNA cloning, sequencing, and recombinant protein expression.
Rhodanese (thiosulfate sulfurtransferase) is expressed at high levels in liver and is involved in the detoxification of cyanide. The full-length cDNA corresponding to the mouse rhodanese gene (Tst), which is located on chromosome 15, was cloned by PCR amplification of a liver cDNA library and subjected to DNA sequencing. Alignment of the rhodanese cDNA sequences from mouse and rat, which we previously cloned (Biochem. J. 275:227-231), revealed 97.3 percent identity at the protein level and 94.6 percent identity at the DNA level. When the mouse and rat cDNAs were expressed under the control of IPTG-inducible promoters in E. coli, the cell extracts exhibited cyanide-metabolizing activity, indicating that both genes encode functional rhodanese molecules.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D051381:Rats; D011994:Recombinant Proteins; D017422:Sequence Analysis, DNA; D013884:Thiosulfate Sulfurtransferase / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D011994:Recombinant Proteins; D013884:Thiosulfate Sulfurtransferase