Fluorometric assay of B6 vitamers in biological material.
A method for the separation and assay of all B6 vitamers from a single sample of serum is reported. A new reagent system containing sodium glyoxalate, potassium aluminum, sulfate and manganese dioxide was used for quantitative conversion of pyridoxamine and its phosphate to the corresponding aldehydes by a combination of transamination and oxidation reactions.
D006801:Humans; D009943:Organophosphorus Compounds / Q000097:blood; D011730:Pyridoxal / Q000097:blood; D011732:Pyridoxal Phosphate / Q000097:blood; D011733:Pyridoxamine / Q000031:analogs & derivatives / Q000097:blood; D011736:Pyridoxine / Q000097:blood* / Q000302:isolation & purification
D009943:Organophosphorus Compounds; D011730:Pyridoxal; D011732:Pyridoxal Phosphate; D011733:Pyridoxamine; D011736:Pyridoxine; C010627:pyridoxamine phosphate