An improved technique for brain microdissection and subsequent histological control.
A microdissection technique is described which allows removal of discrete brain regions in the cryostat under stereotaxic control. In addition, thin slices (20 micron) from the upper and lower surfaces of the dissected tissue can be preserved for subsequent staining and assessment of possible contamination by surrounding tissue.
D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D001931:Brain Mapping / Q000295:instrumentation*; D001933:Brain Stem / Q000033:anatomy & histology; D002531:Cerebellum / Q000033:anatomy & histology; D004210:Dissection; D009434:Neural Pathways / Q000033:anatomy & histology; D051381:Rats