Auxiliary method for clonal identification of Staphylococcus aureus by protein band pattern of released proteins on SDS-polyacrylamide gel.
A supportive method for clonal identification of Staphylococcus aureus strains was devised. Culture supernatant obtained by cellophane surface culture was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) without performing any concentration procedure prior to electrophoresis. The combined use of cellophane surface culture and SDS-PAGE was convenient for determining whether the strains belonged to the same clone or not when conducted in conjunction with other tests for bacteriological characterization.
D001426:Bacterial Proteins / Q000032:analysis*; D015373:Bacterial Typing Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D013211:Staphylococcus aureus / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism
D001426:Bacterial Proteins