Assignment of the growth hormone receptor gene to bovine chromosome 20 using linkage analysis and somatic cell mapping.
A polymorphism was identified in the bovine growth hormone receptor (GHR) gene by digesting polymerase chain reaction (PCR) products with the restriction enzyme Alul. Two alleles were segregating in cattle of Bos indicus descent, but one allele appears to be fixed in Bos taurus cattle. GHR was localized to bovine chromosome 20 using bovine-rodent hybrid cell lines and linkage analysis.
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific; D008040:Genetic Linkage; D005819:Genetic Markers; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D011986:Receptors, Somatotropin / Q000235:genetics*; D015183:Restriction Mapping; D012377:Rodentia
D017931:DNA Primers; D005819:Genetic Markers; D011986:Receptors, Somatotropin; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific