Inhibition of lipoxygenase in lentil protoplasts by expression of antisense RNA.
A number of plasmids were constructed containing chimeric genes consisting of fragments of antisense-oriented lentil lipoxygenase cDNA. The different constructs were tested for their ability to lower lipoxygenase activity in lentil protoplasts. Plasmids containing a full length lentil lipoxygenase cDNA proved to be the most effective, reducing the activity of the target enzyme to 70% of the control value. On the other hand, the full length lentil lipoxygenase cDNA in the sense orientation yielded a 20% increase of lipoxygenase activity.
D018076:DNA, Complementary; D007887:Fabaceae / Q000201:enzymology*; D008084:Lipoxygenase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D016859:Lipoxygenase Inhibitors; D010946:Plants, Medicinal; D010957:Plasmids; D011523:Protoplasts / Q000201:enzymology; D016372:RNA, Antisense / Q000096:biosynthesis*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping
D018076:DNA, Complementary; D016859:Lipoxygenase Inhibitors; D016372:RNA, Antisense; D011993:Recombinant Fusion Proteins; D008084:Lipoxygenase