title: The properties of the multicopy suppressor of the ogd1 mutation in yeast.
abstract: The 8.1 kb chromosomal fragment partially suppressing the ogd1 mutation in Saccharomyces cerevisiae has been cloned. The molecular analysis revealed that its suppressor gene codes for a natural glutamine tRNA(CAG) and maps on chromosome XIII in the upstream region of the URA10 gene. The multicopy plasmids containing this tRNA gene also suppressed the standard trp1-1 amber mutation and conferred the sensitivity of yeast cells to paromomycin and increased temperature.
mesh_terms: D002874:Chromosome Mapping; D015825:Chromosomes, Fungal; D003001:Cloning, Molecular; D005800:Genes, Fungal; D016153:Genes, Suppressor; D005990:Glycerol / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D010303:Paromomycin / Q000494:pharmacology; D014176:Protein Biosynthesis; D012351:RNA, Transfer, Gln / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D013696:Temperature
chemical_list: D012351:RNA, Transfer, Gln; D010303:Paromomycin; D007655:Ketoglutarate Dehydrogenase Complex; D005990:Glycerol