title: [A full-size DNA copy of the tick-borne encephalitis virus genome. I. Analysis of the 5'- and 3'-terminal noncoding regions of the genome].
abstract: Using reverse transcription and the polymerase chain reaction, cDNA fragments of noncoding regions of the tick-borne encephalitis virus (TBEV) genome were obtained. These fragments were cloned into a pGEM3 vector, and their nucleotide sequences were determined. The heterogeneity of the 3'-terminal untranslated region of the TBEV RNA was revealed. To create a stable full-size DNA copy of the TBEV genome, four cDNA variants differing in length and structure of the 3'-terminal fragment of the viral RNA were cloned into a pBR322-derived vector.
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary; D004279:DNA, Viral; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010957:Plasmids; D012367:RNA, Viral / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D012367:RNA, Viral
vernacular_title: Polnorazmernaia DNK-kopiia genoma virusa kleshchevogo éntsefalita. I. Analiz 5'- i 3'-kontsevykh nekodiruiushchikh oblasteĭ genoma.