[Effect of an inducer on orientation of the DNA-binding domain of the Lac repressor].
We have identified the lac repressor amino acids which form cross-links with partially depurinated DNA and hence are proximate to it. Only lysine-33 is cross-linkable in specific complex with lac operator. At the presence of inducer molecules lysine-33 remains cross-linkable. However, the N-terminal alpha-aminogroup was additionally cross-linked with the boundaries of lac operator. We conclude that orientation of the DNA-binding domain of the repressor is reversed by inducer binding.
D000595:Amino Acid Sequence; D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D018257:Helix-Loop-Helix Motifs; D007763:Lac Operon; D008239:Lysine / Q000378:metabolism; D008969:Molecular Sequence Data; D012097:Repressor Proteins / Q000378:metabolism*
D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D004247:DNA; D008239:Lysine
Vliianie induktora na orientatsiiu DNK-sviazyvaiushchikh domenov Lac-repressora.