Intrafamily fragment analysis of the T cell receptor beta chain CDR3 region.
The length distributions of the third complementarity determining region (CDR3) of the T cell receptor (TCR) beta chain were determined quantitatively from peripheral blood lymphocytes of healthy donors. RT-PCR products of 26 V beta families and subfamilies were analyzed by an A.L.F. DNA sequencer and Fragment Manager software. We established a normal reference of CDR3 lengths for most of the V beta families and subfamilies. The known range of CDR3 lengths for the V beta chain was expanded to 24 amino acids, and quantitative measurements were made for each fragment length allowing intrafamily CDR3 fragment length comparisons. Importantly, we were able to analyze intrafamily CDR3 fragment distribution without optimizing PCR conditions, thereby circumventing a major obstacle found in intrafamily TCR beta chain comparisons.
D000328:Adult; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000032:analysis* / Q000235:genetics; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry* / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology
D000596:Amino Acids; D018076:DNA, Complementary; D010446:Peptide Fragments; D012333:RNA, Messenger; D017260:Receptor-CD3 Complex, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta