Nucleotide changes in oxidatively stressed lymphocytes.
Similar to HIV-1-induced suppression of thymus-derived lymphocytes (T cells), oxidatively stressed T cells show inhibited DNA synthesis and proliferation. The influence of oxidative stress on nucleotide pools was explored using 3H-uridine addition to OKT3-stimulated peripheral blood lymphocytes. The cells were preincubated and stimulated in the presence of 1 mM buthionine sulfoximine to inhibit GSH synthesis. This treatment gave rise to a significant reduction in dUDP and TTP biosynthesis following 18-32 hours stimulation, indicating possible impairment of ribonucleotide reductase activity.
D019328:Buthionine Sulfoximine; D002453:Cell Cycle; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003856:Deoxyuracil Nucleotides / Q000097:blood; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005978:Glutathione / Q000097:blood; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000494:pharmacology; D016853:Muromonab-CD3 / Q000276:immunology; D009711:Nucleotides / Q000097:blood*; D018384:Oxidative Stress / Q000502:physiology*; D013942:Thymine Nucleotides / Q000097:blood; D014529:Uridine / Q000097:blood
D003856:Deoxyuracil Nucleotides; D004791:Enzyme Inhibitors; D016853:Muromonab-CD3; D009711:Nucleotides; D013942:Thymine Nucleotides; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D005978:Glutathione; C024157:thymidine 5'-triphosphate; D014529:Uridine