Identification of parietal cells in gastric body mucosa with HMFG-2 monoclonal antibody.
OBJECTIVE
To identify parietal cells in the upper gastrointestinal tract by an immunoperoxidase method, using commercially available monoclonal antibodies.


METHODS
Routine surgical biopsy specimens of gastric body mucosa were examined using the avidin-biotin peroxidase method with the monoclonal antibodies HMFG-1 and HMFG-2 to identify parietal cells. Double immunoperoxidase labelling with HK12.18, a well characterised monoclonal antibody directed against an epitope on the alpha (catalytic) subunit of H+ translocating, K+ stimulated adenosine triphosphatase (H,K-ATPase), was used to confirm that HMFG-1 and -2 stained parietal cells.


RESULTS
HMFG-1 and HMFG-2 showed consistent parietal cell staining patterns in the gastric body mucosa. HMFG-2 gave a more intense staining pattern of the secretory canaliculi. This was confirmed by double immunolabelling with HK12.18.


CONCLUSIONS
HMFG monoclonal antibodies are recommended as highly specific markers of human gastric parietal cells.
D000911:Antibodies, Monoclonal; D005753:Gastric Mucosa / Q000276:immunology; D017506:H(+)-K(+)-Exchanging ATPase / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D018396:Mucin-1 / Q000276:immunology; D010295:Parietal Cells, Gastric / Q000166:cytology* / Q000276:immunology
D000911:Antibodies, Monoclonal; D018396:Mucin-1; D017506:H(+)-K(+)-Exchanging ATPase