Configuration of latent varicella-zoster virus DNA.
The configuration of latent varicella-zoster virus (VZV) DNA was analyzed by PCR. Template DNA for both internal and terminal VZV primers was present in a 1:1 ratio in ganglionic DNA, compared with a 15:1 ratio in DNA extracted from VZV virions, indicating that the VZV genomic termini are adjacent in latently infected human ganglia.
D000328:Adult; D000368:Aged; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D004279:DNA, Viral / Q000737:chemistry* / Q000302:isolation & purification; D005260:Female; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012668:Trigeminal Ganglion / Q000821:virology*; D017735:Virus Latency
D017931:DNA Primers; D004279:DNA, Viral