Plasmodium vivax: detection of blood parasites using fluorochrome labelled monoclonal antibodies.
A monoclonal antibody (MoAb) 2C6111 specific for Plasmodium vivax erythrocytic stages was shown to detect parasitized erythrocytes in blood samples collected in the field. This MoAb binds to the mature trophozoite, schizont and gametes of P. vivax and upon examination of 43 wild isolates no evidence of polymorphism was found. To search for P. vivax parasites in human blood a MoAb immunofluorescent test (MoAb-IFT) was developed. The assay is based on the ability of fluorescein isothiocyanate labelled MoAb 2C6111 to combine with parasitized erythrocytes on thin blood smears. A preliminary field trial was carried out in Venezuela to determine the usefulness of MoAb-IFT for the specific diagnosis of P. vivax malaria. Blood samples collected from malarious and non-malarious individuals were examined both by standard microscopy of Giemsa stained thick blood smears (G-TS) and MoAb-IFT. The latter was specific and gave a 100% correlation with G-TS. Sensitivity was close to that usually achieved with Giemsa stained blood films.
D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan / Q000032:analysis; D004912:Erythrocytes / Q000469:parasitology; D016650:Fluorescein-5-isothiocyanate; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006801:Humans; D015151:Immunoblotting; D016780:Malaria, Vivax / Q000097:blood / Q000175:diagnosis*; D051379:Mice; D008807:Mice, Inbred BALB C; D018512:Parasitemia / Q000175:diagnosis*; D010966:Plasmodium vivax / Q000276:immunology*; D012680:Sensitivity and Specificity; D014687:Venezuela
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D005456:Fluorescent Dyes; D016650:Fluorescein-5-isothiocyanate