Methodology for the assessment of lung protection. Human pulmonary artery endothelial cell preservation using haemaccel.
This investigation was designed to show an original methodology for the assessment of lung preservation and to analyze the efficacy of a low potassium polygelin solution (haemaccel [HM]) on isolated human pulmonary artery endothelial cells. The effects of HM were compared with those of low potassium dextran (LPD), Belzer (University of Wisconsin [UWS]), and Euro-Collins solutions. The viability of the endothelial cultures was assessed by means of both total protein content and recovery of metabolic cellular function expressed as the protein synthesis rate after 6 hr and 16 hr of incubation at 10 degrees C. Our results failed to show any significant difference in the total protein content for HM, LPD, and UWS, both after 6 and 16 hr of incubation; however, the Euro-Collins-preserved sample revealed a significant drop in this parameter as early as 6 hr after the start. This finding was regarded as a clear indication of cellular cytotoxicity. In contrast, the metabolism recovery capacity of the cells varied significantly between HM and UWS at 6 hr and among HM, LPD, and UWS at 16 hr; at 6 hr, however, no significant difference was observed between HM and LPD. In conclusion, HM appears to exert a more significant effect on human pulmonary artery endothelial cell metabolism recovery than do the other fluids, thus suggesting its suitability as a long-term pulmonary perfusate.
D002455:Cell Division; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007930:Leucine / Q000378:metabolism; D008168:Lung; D009926:Organ Preservation / Q000379:methods*; D010952:Plasma Substitutes; D011097:Polygeline; D014176:Protein Biosynthesis; D011651:Pulmonary Artery; D011865:Radioisotope Dilution Technique; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods; D014316:Tritium
D010952:Plasma Substitutes; D014316:Tritium; D011097:Polygeline; D007930:Leucine