title: Kinetics of interferon gamma production in vivo during infection with the S48 vaccine strain of Toxoplasma gondii.
abstract: The kinetics of interferon gamma (IFN gamma) production in vivo was examined in sheep during a primary and secondary infection with the sheep vaccine strain (S48) of Toxoplasma gondii. Efferent lymph plasma from a node draining the site of inoculation was tested for anti-viral activity which could be neutralized with monoclonal antibodies against IFN gamma. Within 2 to 5 days of primary infection IFN gamma was detected in each of five sheep and persisted for 6 to 9 days. Accelerated production of IFN gamma occurred after secondary infection, the cytokine being detected in the first 24 h, and persisting in lymph for a further 4-5 days. From day 6 onwards after primary infection, efferent lymph cells produced IFN gamma when stimulated in vitro with a crude T. gondii antigen. These results show that IFN gamma is induced in sheep after infection with the S48 strain of T. gondii.
mesh_terms: D000818:Animals; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000378:metabolism; D007700:Kinetics; D008196:Lymph / Q000276:immunology; D016052:Protozoan Vaccines / Q000276:immunology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology* / Q000378:metabolism / Q000517:prevention & control
chemical_list: D016052:Protozoan Vaccines; D007371:Interferon-gamma