Pharmacological effects of dai-saiko-to on lipid biosynthesis in cultured human hepatocyte HepG2 cells.
The pharmacological effects of Dai-saiko-to, a Japanese and Chinese traditional medicinal mixture (Kampohozai), on lipid biosynthesis were investigated in cultured human hepatocyte HepG2 cells. The addition of Dai-saiko-to (0.5 mg/ml), which had no significant effect on cell proliferation, caused a marked decrease in the intracellular triglyceride content with no significant changes in the other lipid fraction. At the same time, the incorporation of 14C-acetate or 3H-glycerol into the triglyceride or diglyceride fractions also decreased significantly. These results suggest that Dai-saiko-to decreases hepatic triglyceride biosynthesis, which might contribute to a reduction in plasma VLDL levels.
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002681:China; D002784:Cholesterol / Q000096:biosynthesis / Q000378:metabolism; D004075:Diglycerides / Q000096:biosynthesis / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D006949:Hyperlipidemias / Q000188:drug therapy; D007564:Japan; D050356:Lipid Metabolism; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D010743:Phospholipids / Q000096:biosynthesis / Q000378:metabolism; D010936:Plant Extracts; D018517:Plant Roots / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis / Q000378:metabolism
D004075:Diglycerides; D004365:Drugs, Chinese Herbal; D008055:Lipids; D010743:Phospholipids; D010936:Plant Extracts; D014280:Triglycerides; C051121:daisaikoto; D002784:Cholesterol