A novel Cl(-)-pump: intracellular regulation of transport activity.
Utilizing a purified basolateral plasma membrane vesicle preparation containing Cl(-)-ATPase, it was demonstrated that ATP, and its subsequent hydrolysis, stimulated both intravesicular Cl- accumulation and intravesicular negativity with almost identical kinetics. Mg2+ also stimulated both pump transport characteristics with an optimal concentration of 3 mM. Similarly, the pH optimum for both pump transport expressions was 7.8. Orthovanadate inhibition of both pump transport characteristics was directly related to its concentration. These results suggested that the active Cl- transport mechanism is electrogenic and is driven by a Cl(-)-stimulated ATPase.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D027321:Anion Transport Proteins; D001048:Aplysia; D001692:Biological Transport; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology / Q000502:physiology; D002499:Centrifugation, Density Gradient; D002712:Chlorides / Q000378:metabolism*; D004848:Epithelium / Q000201:enzymology; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008564:Membrane Potentials; D014638:Vanadates / Q000494:pharmacology
D027321:Anion Transport Proteins; D002413:Cations, Divalent; D002712:Chlorides; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D014638:Vanadates; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C031652:anion-sensitive ATPases; D008274:Magnesium