Membrane fluidity of Escherichia coli during heat-shock.
The excimer-forming fluorophore dipyrenylpropane has been used to measure the relative fluidity of total membranes isolated from Escherichia coli grown at 30 or 45 degrees C, or exposed to a heat-shock from 30 to 45 degrees C for various periods of time. Parallel experiments were performed using [35S]methionine pulse-labeling of cells, to study the induction of heat-shock proteins (HSPs) at different times after the sudden change in E. coli growth-temperature from 30 to 45 degrees C. Results suggest that upon an abrupt temperature upshift from 30 to 45 degrees C, membrane fluidity adjustment to the steady-state level at the high temperature, takes place during the E. coli heat-shock response.
D004926:Escherichia coli / Q000502:physiology*; D005456:Fluorescent Dyes; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D018869:Heat-Shock Response; D007202:Indicators and Reagents; D008560:Membrane Fluidity; D011721:Pyrenes; D013997:Time Factors
D005456:Fluorescent Dyes; D006360:Heat-Shock Proteins; D007202:Indicators and Reagents; D011721:Pyrenes; C035116:1,3-di-(1-pyrenyl)propane