Emergence of virus escape mutants after immunization with epitope vaccine.
BALB/c and C57BL/6J mice were immunized with recombinant vaccines consisting of lymphocytic choriomeningitis virus CD8+ T-lymphocyte epitopes and a carrier protein. During challenge infection with WE strain lymphocytic choriomeningitis virus, mutants with alterations in distinct amino acid residues of the epitopic nonapeptides appeared and multiplied. Splenocytes from WE-infected BALB/c mice lysed cells coated with the WE-type epitope; lysis was considerably less effective when the epitopic nonapeptide with which the syngeneic cells had been sensitized was the mutated form. Neither target was lysed by splenocytes from BALB/c mice infected with the variant virus. Mutants were not detected in F1 hybrid mice immunized with two viral epitopes that were restricted by class I molecules of both parents.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002213:Capsid / Q000737:chemistry / Q000276:immunology; D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry; D000939:Epitopes / Q000276:immunology*; D005260:Female; D008217:Lymphocytic choriomeningitis virus / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016133:Polymerase Chain Reaction; D014614:Vaccines, Synthetic; D014758:Viral Core Proteins / Q000737:chemistry / Q000276:immunology; D014765:Viral Vaccines
D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral; D000939:Epitopes; D014614:Vaccines, Synthetic; D014758:Viral Core Proteins; D014765:Viral Vaccines