A rapid and non-invasive selection of transgenic embryos before implantation using green fluorescent protein (GFP).
Non-invasive selection of transgenic mice was performed at the stage of preimplantation embryos. The morulae collected from wild female mated with hemizygous transgenic male expressing Aequorea victoria green fluorescent protein (GFP) under chicken beta-actin promoter could be classified as green or non-green under a fluorescent microscope. All the green embryos were shown to carry the transgene by PCR analysis. Taking advantage of the detection of GFP expression can be done non-invasively, the selected embryos were demonstrated to be able to developed to term with 100% of accuracy of the selection.
D000818:Animals; D001483:Base Sequence; D001755:Blastocyst / Q000166:cytology* / Q000502:physiology*; D017931:DNA Primers; D005260:Female; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence / Q000379:methods; D008969:Molecular Sequence Data; D009924:Organ Culture Techniques; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D015203:Reproducibility of Results
D017931:DNA Primers; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins