Synaptic loss in the proximal axon of anterior horn neurons in motor neuron disease.
This report deals with an ultrastructural investigation of the synapses of the proximal axons of normal-appearing anterior horn neurons of 7 patients with amyotrophic lateral sclerosis (ALS) and 4 patients with motor neuron disease who had no upper motor neuron and corticospinal tract involvement (lower motor neuron disease, LMND). Specimens from 12 age-matched individuals who died of non-neurological diseases served as controls. Proximal axons directly emanating from the normal-appearing neurons were examined: 42 axons were from ALS patients, 43 from LMND patients and 87 from controls. Our results show that the number of synapses on axon hillocks, as well as the lengths of the synaptic contact and of the active zone were reduced in both groups of patients (P < 0.0001), but no significant differences were seen between patients and controls with respect to the synaptic parameters of initial axon segments. There was no overall difference between ALS and LMND patients. These findings suggest that the electrophysiological functions pertaining to integration of electrical inputs into the axon and information transduction on the axon may be greatly impaired in the early stages of motor neuron diseases, and that the observed synaptic alterations may be pathological events, likely to be due to anterior horn neuron degeneration.
D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000473:pathology*; D000870:Anterior Horn Cells / Q000473:pathology*; D001369:Axons / Q000648:ultrastructure*; D002452:Cell Count; D006801:Humans; D008875:Middle Aged; D016472:Motor Neuron Disease / Q000473:pathology*; D009474:Neurons / Q000648:ultrastructure; D013569:Synapses / Q000648:ultrastructure*