Cloning and characterization of seizure-related gene, SEZ-6.
SEZ-6 is a brain-specific cDNA. Its expression is increased by convulsant drug and it encodes membrane protein with five copies of short consensus repeat (SCR; complement C3b/C4b binding site) and two repeated sequences which are partially similar to CUB domain (complement Clr/s-like repeat). In this study, we analyzed the gene product of SEZ-6 by antibody raised against SEZ-6 peptide. In vitro translation and immunoblot revealed that SEZ-6 protein is modified post-translationally and expressed in the cerebrum and the cerebellum. To analyze the SEZ-6 expression in detail, we searched for other types of SEZ-6 cDNA by the polymerase chain reaction. Two additional types of cDNAs were isolated and sequence analysis showed that one cDNA encodes membrane protein with a different C-terminal region and the other encodes secreted protein with two SCRs and one CUB-like domain.
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D003292:Convulsants / Q000494:pharmacology; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry
D000906:Antibodies; D003292:Convulsants; D017931:DNA Primers; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C093131:Sez6 protein, mouse