title: A new method for direct detection of Listeria monocytogenes from foods by PCR.
abstract: The preparation of DNA by alcohol precipitation in the presence of Na1 was used to enable the direct detection of Listeria monocytogenes in the amount of 10(3) CFU/0.5 g of sample. This procedure produces PCR-quality DNA directly from foods, such as soft cheese and minced meat.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002611:Cheese / Q000382:microbiology; D011232:Chemical Precipitation; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000209:etiology; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics* / Q000302:isolation & purification*; D008088:Listeriosis / Q000209:etiology; D008460:Meat / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012974:Sodium Iodide
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012974:Sodium Iodide