Genotypes and virus load in patients with hepatitis C infection.
Virus load was tested by means of PCR calibrated with standards and HCV genotypes were determined by the LIPA-technique using sera from 123 HCV patients. Of these 39 were on renal hemodialysis treatment, 19 suffered from hemophilia, 13 were i.v. drug users and the remaining 52 had none of these risk factors (chronic hepatitis group). The most prevalent subtype in Austria was 1b followed by 3a and 1a. However, genotype 1b infections were found relatively less often in hemophilia patients and drug users than in the other groups, indicating that hemophiliacs probably had been infected by an antihemophilic plasma coming from South American or Asian donors. The highest amounts of virus were found in patients infected with genotype 3a. Determination of the patient's virus load and of the infecting subtype of HCV may be helpful in planning interferon alpha therapy.
D002908:Chronic Disease; D004279:DNA, Viral / Q000032:analysis; D005838:Genotype; D006467:Hemophilia A / Q000150:complications; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000209:etiology / Q000821:virology*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000150:complications; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000097:blood / Q000302:isolation & purification; D006435:Renal Dialysis; D012307:Risk Factors; D015819:Substance Abuse, Intravenous / Q000150:complications; D014766:Viremia / Q000821:virology*
D004279:DNA, Viral; D012367:RNA, Viral