Interleukin 3-dependent, asialo-GM1-positive cell line with natural cytotoxic but without natural killer cytotoxic activity.
IL-3-dependent cell line (designated as A-3) from adherent spleen cells was developed. The surface phenotype of A-3 cells was analysed by flow cytometry and determined as ASGM1+, FcR+, Ia+. There was no expression of T cell markers (Th1-CD3-CD4-CD8-), B cell marker (surface Ig) as well as macrophage marker Mac-1 antigen. Although A-3 cells expressed ASGM1, they are lacking NK cytotoxic activity in 4 h Cr release assay with YAC-1 targets. In contrast, A-3 cells are endowed with NC cytotoxic activity as shown in 18 h Cr release assay with WEHI-164 target cells.
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002469:Cell Separation; D003094:Collagen; D003602:Cytotoxicity, Immunologic; D005260:Female; D005434:Flow Cytometry; D005677:G(M1) Ganglioside / Q000032:analysis*; D016130:Immunophenotyping; D007377:Interleukin-3 / Q000494:pharmacology*; D016131:Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology*
D007377:Interleukin-3; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D003094:Collagen