Agar gel electrophoretic determination of glycosylated hemoglobin: effect of variant hemoglobins, hyperlipidemia, and temperature.
We investigated the effect of temperature, variant hemoglobins, and hyperlipidemia on determination of glycosylated hemoglobin by an electrophoretic method (Clin. Chem. 26: 1598-1602, 1980). We found that: (a) temperature variations ranging from 4 to 30 degrees C were without effect on results obtained by electrophoresis; (b) concurrent determination of glycosylated hemoglobin by electrophoresis and column-chromatography in blood specimens from 150 diabetic patients yielded almost identical mean values for both procedures when operations were carried out at 22 degrees C; (c) electrophoretic determination of glycosylated hemoglobin in whole-blood hemolysate was not affected by concentration of triglycerides; and (d) unlike column-chromatographic procedures, which underestimate the percentage of glycosylated hemoglobin in patients with hemoglobin S and C, the electrophoretic method accurately determined the proportion of glycosylated hemoglobin in these hemoglobinopathies. Evidently, electrophoresis on agar gel is an excellent alternative to cation-exchange column-chromatographic methods for glycosylated hemoglobin.
D002852:Chromatography, Ion Exchange; D003920:Diabetes Mellitus / Q000097:blood; D004586:Electrophoresis; D004587:Electrophoresis, Agar Gel; D006441:Hemoglobin A / Q000032:analysis; D006444:Hemoglobin C / Q000031:analogs & derivatives* / Q000032:analysis; D006451:Hemoglobin, Sickle / Q000031:analogs & derivatives* / Q000032:analysis; D006453:Hemoglobinopathies / Q000097:blood*; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D013696:Temperature; D006442:Glycated Hemoglobin
D006441:Hemoglobin A; D006444:Hemoglobin C; D006451:Hemoglobin, Sickle; C024240:glycated hemoglobin C; D006442:Glycated Hemoglobin; C024241:glycated hemoglobin S; C028881:hemoglobin A(0)