Odor-induced currents in Xenopus olfactory receptor cells measured with perforated-patch recording.
1. Odor-evoked currents were recorded in Xenopus laevis olfactory receptor neurons (ORNs) by the use of conventional, as well as nystatin and gramicidin-perforated, whole cell recording. The odor-evoked current ran down quickly in conventional, but not in perforated, whole cell recording. All three types of recording gave similar values for the amplitude, latency, time-to-peak, recovery time, and reversal potential of the odor-evoked current. 2. A secondary Cl current comprised a significant part of the odor-evoked current (55-65%). ECl measured by gramicidin perforation, which does not alter [Cl-]i, was -2.3 +/- 5.0 (SE) mV, indicating that these neurons maintain a high [Cl-]i and that the secondary Cl current plays an excitatory role in olfactory transduction.
D000818:Animals; D018118:Chloride Channels / Q000187:drug effects / Q000378:metabolism; D006096:Gramicidin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D009475:Neurons, Afferent / Q000502:physiology*; D009761:Nystatin / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D018035:Receptors, Odorant / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D012903:Smell / Q000502:physiology*; D013268:Stimulation, Chemical; D014982:Xenopus laevis
D018118:Chloride Channels; D007476:Ionophores; D018035:Receptors, Odorant; D009761:Nystatin; D006096:Gramicidin