Sialyl Lewis X moiety on rat polymorphonuclear leukocytes responsible for binding to rat E-selectin.
We investigated the presence of the carbohydrate ligand for E-selectin on the cell surface of rat polymorphonuclear leukocytes (PMN). Rat PMN, isolated from peripheral blood, adhered to recombinant rat E-selectin-coated microplates. The adhesion was inhibited either by an anti-rat E-selectin monoclonal antibody (MAb), a sialyl Lewis X (SLex) oligosaccharide or neuraminidase digestion. FACS analysis revealed the expression of SLex as well as other adhesion molecules such as L-selectin, CD11a, CD11b and CD18 on the cell surface of rat PMN. The binding of an anti-SLex MAb KM93 to rat PMN was inhibited competitively by a SLex but not by a Lewis X (Lex). The reactivities of two anti-SLex MAbs, KM93 and CSLEX-1, or an anti-Lex MAb BC90/45 to rat PMN differed from those to human PMN. These results suggest that rat PMN contain the SLex-moiety, which binds to rat E-selectin and is different from that of human PMN.
D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D002448:Cell Adhesion / Q000187:drug effects; D002462:Cell Membrane / Q000502:physiology; D019040:E-Selectin / Q000378:metabolism*; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D016256:Lewis X Antigen / Q000502:physiology*; D008297:Male; D009439:Neuraminidase / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology*; D009844:Oligosaccharides / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000378:metabolism; D000080506:Sialyl Lewis X Antigen
D000911:Antibodies, Monoclonal; D019040:E-Selectin; D016256:Lewis X Antigen; D009844:Oligosaccharides; D011994:Recombinant Proteins; D000080506:Sialyl Lewis X Antigen; D009439:Neuraminidase