Biochemical identification of the bovine blood group M' antigen as a major histocompatibility complex class I-like molecule.
Absorption and elution experiments showed that it was impossible to separate antibodies against blood group factor M' from antibodies against bovine lymphocyte antigen (BoLA) A16 in an antiserum showing haemolytic activity against M' as well as lymphocytotoxic activity against BoLA-A16. To elucidate the structural relationship between BoLA-A16 and blood group antigen M', immunoprecipitation experiments on red and white cell lysates isolated from M'-A16 positive and negative cattle were carried out. These results showed that M(r) 44,000 and M(r) 12000 polypeptides can be precipitated from both red and white cells isolated from M'-A16 positive animals, whereas no bands were seen in M'-A16 negative animals in precipitations with the same antibody. Precipitation with a crossreacting human beta 2-microglobulin (beta 2-m) specific antibody confirmed a class-I-like structure associated with beta 2-m on M' positive red cells and the absence of such a structure on M' negative red cells. Sequential precipitations gave analogous results. Proteolytic degradation by papain and V8 protease did not reveal any substantial difference between red and white M'-A16 positive cells, but a slight difference in the pI of the immunoprecipitable components of red and white cells was observed. All together, this indicates that either the blood group antigen M' is the BoLA-A16 class I antigen or M' and BoLA-A16 are two different class I polypeptides with the same relative mass, sharing identical epitopes and both associated with beta 2-m. Comparable results were obtained with M1 and BoLA-A24.
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002417:Cattle; D003429:Cross Reactions; D004912:Erythrocytes / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000097:blood*; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D001613:beta 2-Microglobulin / Q000276:immunology
D000906:Antibodies; D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I; D001613:beta 2-Microglobulin