Defects of copper deficiency in rats are modified by dietary treatments that affect glycation.
We examined the hypothesis that nonenzymatic glycosylatin of proteins (glycation) contributes to the defects of copper deficiency. We studied copper-adequate and -deficient rats while altering two factors known to affect glycation: type of dietary carbohydrate and amount of food intake. Copper deficiency caused cardiac enlargement and anemia, decreased erythrocyte osmotic fragility, enhanced heart lipid peroxidation, increased the percentage of glycated hemoglobin (Hb A1) and reduced staining of lens crystallins on SDS-PAGE gels (suggestive of glycation). Increasing dietary sucrose reduced organ copper concentration, exacerbated the rise in Hb A1 and worsened the anemia caused by copper deficiency. Food restriction ameliorated heart and erythrocyte defects, reduced the percentage of glycated hemoglobin and heart peroxidation and also improved heart and liver copper status in copper-deficient rats. These findings indicate that copper deficiency enhances glycation and that sucrose may exacerbate some defects of copper deficiency by enhancing glycation. Inhibition of defects of copper deficiency by food restriction suggests that glycation and/or peroxidation may contribute to those defects.
D000704:Analysis of Variance; D000818:Animals; D001806:Blood Urea Nitrogen; D001835:Body Weight; D002118:Calcium / Q000032:analysis; D003300:Copper / Q000032:analysis* / Q000172:deficiency*; D004032:Diet / Q000592:standards; D004040:Dietary Carbohydrates / Q000494:pharmacology*; D004435:Eating / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D004906:Erythrocyte Count; D006442:Glycated Hemoglobin / Q000032:analysis / Q000378:metabolism; D006031:Glycosylation; D006400:Hematocrit; D006454:Hemoglobins / Q000032:analysis / Q000378:metabolism; D007501:Iron / Q000032:analysis; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D009206:Myocardium / Q000737:chemistry; D009929:Organ Size; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000378:metabolism
D002118:Calcium; D003300:Copper; D004040:Dietary Carbohydrates; D006442:Glycated Hemoglobin; D006454:Hemoglobins; D007501:Iron; D011506:Proteins; D017392:Thiobarbituric Acid Reactive Substances