Molecular cloning of a novel protein regulated by opioid treatment of NG108-15 cells.
A new cDNA clone, NGD5, has been identified from a subtraction cDNA library constructed with mRNA isolated from control neuroblastoma x glioma NG108-15 cells and cells treated for 48 h with the delta-opioid agonist, D-Ala2, D-Leu5 enkephalin (DADLE). NGD5 mRNA is decreased, in a naloxone-reversible manner, upon long-term treatment of NG108-15 cells with DADLE, indicating that this clone may be related to opioid receptor function. Northern analysis indicates that NGD5 mRNA is expressed in rat brain. Two similar nearly full-length NGD5 clones, NGD5A and NGD5B, were isolated from a lambda gt10 NG108-15 cDNA library and sequenced. The predicted 40-kDa peptide encoded for by the NGD5 cDNA has no significant homology to the recently cloned mu, delta or kappa opioid receptors nor to any other known proteins.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D015536:Down-Regulation / Q000187:drug effects; D016308:Enkephalin, Leucine-2-Alanine / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051381:Rats; D017465:Receptors, Opioid, delta / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D017465:Receptors, Opioid, delta; D016308:Enkephalin, Leucine-2-Alanine