Immunogenicity of influenza A virus N2 neuraminidase produced in insect larvae by baculovirus recombinants.
Influenza A virus neuraminidase (NA) from A/Udorn/72 (H3N2) was expressed by recombinant baculovirus-infected insects. The recombinant NA was enzymatically active. Enzyme activity was neutralized by polyclonal antisera raised against virion-extracted NA. NA produced in whole insects by a baculovirus expression system is antigenically indistinguishable from virion NA by polyclonal antisera in functional assays (NI) and in ELISA, and is highly immunogenic without adjuvant. It is equivalent in immunogenicity to NA purified from influenza virus. Our results indicate that baculovirus-produced NA could be used as a source for large quantities of purified N2-NA for vaccine use.
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D003001:Cloning, Molecular; D004285:Dogs; D005260:Female; D007106:Immune Sera; D009980:Influenza A virus / Q000201:enzymology* / Q000276:immunology / Q000502:physiology; D007814:Larva / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology*; D009500:Neutralization Tests; D017924:Nucleopolyhedroviruses / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D011995:Recombination, Genetic; D018411:Spodoptera; D010948:Viral Plaque Assay
D007106:Immune Sera; D011994:Recombinant Proteins; D009439:Neuraminidase