Transient bisalbuminemia: separation by isoelectric focusing of human albumin fractions linked to different numbers of benzylpenicilloyl groups.
The behavior of modified human serum albumin (HSA) from penicillin-treated patients and in vitro conjugated samples was studied by isoelectric focusing in the absence and presence of 8 M urea. It is shown that samples classified as bisalbuminemic after separation under native conditions display a spectrum of several major bands with decreasing pIs when focusing is done in the presence of 8 M urea. In this spectrum, the distances between two consecutive major bands are as expected from a 2-unit charge difference. Since this result is compatible with the blockage of a lysine residue with a benzylpenicilloyl (BPO) group, each major band can be interpreted as representing an HSA fraction covalently linked to different numbers of benzylpenicilloyl groups. The separation methods presented therefore seem to constitute simple and reliable tools for a detailed assessment of the extent of BPO covalent binding to HSA both in vivo and in vitro.
D044482:Benzeneacetamides; D006801:Humans; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D010400:Penicillin G / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D010406:Penicillins / Q000494:pharmacology; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000032:analysis*; D014508:Urea
D044482:Benzeneacetamides; D010406:Penicillins; D012709:Serum Albumin; C015026:benzylpenicilloyl G; D014508:Urea; D010400:Penicillin G