Ornithine decarboxylase induction during liver regeneration in IRS-1-deficient mice.
We investigated the induction of ornithine decarboxylase during liver regeneration after partial hepatectomy in IRS-1-deficient mice. There were no significant differences in ODC activity or the time course of changes in ODC activity between IRS-1-deficient mice and wild-type mice. PI 3'-kinase activity showed similar increases in both groups of mice. Furthermore, ODC induction in IRS-1 transfected CHO cells was studied after stimulation by addition of FCS. The maximal ODC activity was 2.5-fold greater in IRS-1-transfected CHO cells than in control CHO cells. Our results suggest that the IRS-1 pathway may be involved in ODC induction. The absence of a difference in ODC and PI 3'-kinase activity in the regenerating liver between IRS-1-deficient mice and wild-type mice may have been related to the compensatory effects of IRS-2/pp190 [Araki et al. Nature (1994) 372, 186-190; Tobe et al. J.Biol.Chem. (1995) 270, 5698-5701].
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004790:Enzyme Induction; D006498:Hepatectomy; D006801:Humans; D055504:Insulin Receptor Substrate Proteins; D008099:Liver / Q000201:enzymology*; D008115:Liver Regeneration; D051379:Mice; D018345:Mice, Knockout; D008817:Mice, Mutant Strains; D009955:Ornithine Decarboxylase / Q000096:biosynthesis*; D010750:Phosphoproteins / Q000096:biosynthesis / Q000172:deficiency* / Q000378:metabolism; D011972:Receptor, Insulin / Q000096:biosynthesis / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D014162:Transfection
C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; C526295:Irs1 protein, mouse; D010750:Phosphoproteins; D011994:Recombinant Proteins; D011972:Receptor, Insulin; D009955:Ornithine Decarboxylase