[A preliminary study on the mechanism of against human tongue cancer cell line].
Inverted phase contrast microscope and transmission electron microscope examination reveals that, tumor-draining lymphnode lymphocytes (DNL) conjugated with tumor cells very tightly at 12 hours co-culture. The DNL cell nucleus was seen positioned away from the target cells, whereas the cell mitochondria granules were oriented toward the point of contact with the target cells. The injured target cell had a highly condensed cytoplasm and chromatin and formation of cytoplasmic blebs, which were consistent with an apoptotic cell death rather than with a lytic or necrotic cell death. The mechanism was exocytosis of granules containing a pore-forming protein. Holes formed in the target cell's membrane may allow a lethal substance to enter and to induce its death via an apoptotic mechanism.
D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D014062:Tongue Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured