Proopiomelanocorticotropin (POMC) peptides and lipoprotein lipase activity in vitro.
Effects of alpha-melanocyte-stimulating hormone (alpha-MSH), beta-melanocyte-stimulating hormone (beta-MSH), beta-lipotropin (beta-LPH), and beta-endorphin (beta-EPH) at concentrations from 10(-9) M up to 10(-6) M on human adipose tissue lipoprotein lipase (LPL) were studied in a cell-free system. alpha-MSH and beta-MSH did not exert any effect on LPL; no degradation of these peptides in the incubation medium could be detected by HPLC analysis. beta-LPH and beta-EPH failed to alter enzyme activity. However, HPLC analysis revealed an unspecific rapid degradation of the peptides due to the activity of tissue proteases. Therefore, the protease inhibitors amastatin, antipain, APMSF, and TPCK were tested at concentrations of 10(-5), 10(-4), and 10(-3) M for their efficacy to inhibit degradation. None of the inhibitors was able to substantially reduce proteolysis of beta-LPH, as was the case with amastatin, APMSF, and TPCK for beta-EPH. However, antipain at 10(-4) M preserved at least 20% of the initial peptide concentration from proteolysis up to 150 min. Antipain caused a decrease in lipoprotein lipase activity (LPLA), which was dependent on concentration. The adverse effect of antipain at concentrations of 10(-4) M on LPL was completely abolished by beta-EPH at a concentration of 10(-6) M.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D002474:Cell-Free System; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D006801:Humans; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008079:Lipoproteins, VLDL / Q000096:biosynthesis / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000502:physiology; D009074:Melanocyte-Stimulating Hormones / Q000494:pharmacology; D011333:Pro-Opiomelanocortin / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000494:pharmacology; D014280:Triglycerides / Q000097:blood; D000521:alpha-MSH / Q000494:pharmacology; D001615:beta-Endorphin / Q000494:pharmacology; D008083:beta-Lipotropin / Q000494:pharmacology
D005230:Fatty Acids, Nonesterified; D008079:Lipoproteins, VLDL; D011480:Protease Inhibitors; D014280:Triglycerides; D000521:alpha-MSH; D001615:beta-Endorphin; D011333:Pro-Opiomelanocortin; D009074:Melanocyte-Stimulating Hormones; D008083:beta-Lipotropin; D008071:Lipoprotein Lipase