Liquid-chromatographic analysis for urinary porphyrins.
Urinary porphyrins are separated, according to number of carboxyl groups, in a system consisting of a mu-Bondapak C18 stationary phase and a mobile phase of methanol and aqueous sodium phosphate (pH 3.5) in a linear gradient. The specimen is prepared simply by adjusting the pH of a 5-mL sample to 2.0 and removing solids by centrifugation. The eluted porphyrins are measured fluorometrically. Naturally occurring non-porphyrin fluorescent substances are eluted ahead of the porphyrins. Chromatography requires about 20 min, and a re-establishment of initial conditions requires an additional 15 min.
D000328:Adult; D002853:Chromatography, Liquid; D003306:Coproporphyrins / Q000652:urine; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011164:Porphyrias / Q000652:urine; D011166:Porphyrins / Q000302:isolation & purification / Q000652:urine*; D012871:Skin Diseases / Q000652:urine; D013050:Spectrometry, Fluorescence; D014578:Uroporphyrins / Q000652:urine
D003306:Coproporphyrins; D011166:Porphyrins; D014578:Uroporphyrins