[A polymorphism study of the DNA extracted from dental tissues].
Often teeth are the only items which can be used for personal identification in forensic medicine. In the present work we describe a method to extract and amplify DNA from dental elements ranging from 2 weeks to 5 year from the avulsion. PCR (polymerase chain reaction) was used to amplify VNTR sequences; the alleles products were electrophoresed, visualized by traditional methods and compared to the amplified products obtained from the matching blood sample. Our results give a new and powerful investigative tool for personal identification in the field of forensic odontostomatology, since such a procedure can be successfully applied both to recent and to ancient teeth.
D001055:Apolipoproteins B / Q000235:genetics; D004247:DNA / Q000032:analysis / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005553:Forensic Dentistry; D006801:Humans; D018598:Minisatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D014070:Tooth / Q000737:chemistry*
D001055:Apolipoproteins B; D004247:DNA
Studio di polimorfismi del DNA estratto da tessuti dentari.