Molecular cloning and characterization of a new member of the RAC protein kinase family: association of the pleckstrin homology domain of three types of RAC protein kinase with protein kinase C subspecies and beta gamma subunits of G proteins.
cDNA clones encoding the third member of the RAC protein kinase family, termed RAC-PK gamma, were isolated from a rat brain cDNA library. The deduced amino acid sequence of RAC-PK gamma was highly related to those of previously identified family members, RAC-PK alpha and beta, that have a pleckstrin homology domain and a protein-serine/threonine kinase catalytic domain at the amino- and carboxyl-terminal regions, respectively. Northern blot analysis indicated that RAC-PK gamma was expressed abundantly in brain and testis. Specific activities of RAC-PK alpha, beta, and gamma purified from transfected COS-7 cells were similar when measured by using myelin basic protein as a phosphate acceptor. Analysis using fusion proteins of glutathione S-transferase revealed that the pleckstrin homology domain of the three subtypes of RAC-PK associate with both protein kinase C subspecies and beta gamma subunits of G proteins. These results suggest that the pleckstrin homology domains of RAC protein kinase family could associate more than one protein to regulate the activity and/or intracellular distribution of this enzyme family by different ways.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000737:chemistry*; D001921:Brain / Q000201:enzymology; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular / Q000379:methods; D016384:Consensus Sequence; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000737:chemistry*; D015723:Gene Library; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D010750:Phosphoproteins; D016133:Polymerase Chain Reaction; D011493:Protein Kinase C / Q000737:chemistry*; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification; D051057:Proto-Oncogene Proteins c-akt; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
D001798:Blood Proteins; D018076:DNA, Complementary; D046911:Macromolecular Substances; D010750:Phosphoproteins; D011994:Recombinant Proteins; C039077:platelet protein P47; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt; D011493:Protein Kinase C; D019204:GTP-Binding Proteins