Cloning and characterization of the murine activin receptor like kinase-1 (ALK-1) homolog.
Multiple serine/threonine kinases were detected in a bone marrow stromal cell line. One of these, the murine activin receptor like kinase-1 (ALK-1) homolog was cloned and sequenced. The expressed recombinant protein (62 kDa) was consistent in size with that predicted by the cDNA (58.6 kDa). On Western blots, a goat polyclonal antibody detected the native ALK-1 protein in bone marrow stromal cells, lung, brain, kidney and spleen. Two protein species of 60 kDa and 72-76 kDa were detected. Glycosylation events or alternative splicing may account for the larger protein specie.
D029404:Activin Receptors; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001483:Base Sequence; D015153:Blotting, Western; D001853:Bone Marrow / Q000378:metabolism; D001854:Bone Marrow Cells; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D006041:Goats / Q000276:immunology; D006801:Humans; D007668:Kidney / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism; D014158:Transcription, Genetic
D000906:Antibodies; D017931:DNA Primers; D010455:Peptides; D011994:Recombinant Proteins; D017346:Protein Serine-Threonine Kinases; D029404:Activin Receptors