[The plasma concentration measurement of local anesthetics, thiobarbiturates and mexiletine by HPLC with automatic pre-treatment system].
The concentrations of lidocaine, its metabolites (MEGX, GX), mepivacaine, bupivacaine, thiamylal, thiopental and mexiletine in plasma have been determined by HPLC method with automatic pre-treatment system. The drugs carried by pre-treatment mobile phase are trapped by hydrophobic interaction on the pre-treatment column (4 mm i.d. x 30 mm L., tetramethylolmethanetriacrylate resin packing). As sending analytical mobile phase which was conditioned with 0.1 M sodium phosphate buffer (pH 2.1) and acetonitrile by switching the flow changeover valve, the absorped drugs were eluted quickly and introduced to the analytical column (6 mm i.d x 150 mm L., octadecylsilan chemical bond sillica packing). On this column, the drugs were separated and analyzed, then measured by a UV spectrophotmetric detector. The time of switching valve, the wave length of detector for measurement and the buffer/acetonitrile ratio of analytical mobile phase were specific for the drugs, respectively. And high recovery and reproducibility were obtained for using auto-injector, so it has been possible to determine the concentrations of drugs in plasma with absolute calibration curves.
D018686:Anesthetics, Intravenous / Q000097:blood*; D000779:Anesthetics, Local / Q000097:blood*; D000889:Anti-Arrhythmia Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008012:Lidocaine / Q000097:blood; D008619:Mepivacaine / Q000097:blood; D008801:Mexiletine / Q000097:blood*; D015203:Reproducibility of Results; D013858:Thiobarbiturates / Q000097:blood*
D018686:Anesthetics, Intravenous; D000779:Anesthetics, Local; D000889:Anti-Arrhythmia Agents; D013858:Thiobarbiturates; D008801:Mexiletine; D008012:Lidocaine; D008619:Mepivacaine