In vivo structural analysis of spliced leader RNAs in Trypanosoma brucei and Leptomonas collosoma: a flexible structure that is independent of cap4 methylations.
The formation of the mRNA 5' end in trypanosomatid protozoa is carried out by trans-splicing, which transfers a spliced leader (SL) sequence and its hypermethylated cap (cap4) from the SL RNA to the pre-mRNA. Previous in vitro studies with synthetic uncapped RNAs have shown that the SL sequence of Leptomonas collosoma can assume two alternate conformations, Form 1 and Form 2, with Form 1 being the dominant one. To gain information about the structure of the SL RNA in vivo, in its protein-rich environment, we have used permeable Trypanosoma brucei and L. collosoma cells for chemical modification experiments. We introduce the use in vivo of the water-soluble reagents CMCT and kethoxal. In contrast to the in vitro results, the Form 2 secondary structure predominates. However, there are chemically accessible regions that suggest conformational flexibility in SL RNPs and a chemically inaccessible region suggestive of protection by protein or involvement in tertiary interactions. Using complementary 2'-O-methyl RNA oligonucleotides, we show that T. brucei SL RNA can be induced to switch conformation in vivo. SL RNA stripped of proteins and probed in vitro does not display the same Form 2 bias, indicating that SL RNA structure is determined, at least in part, by its RNP context. Finally, the methyl groups of the cap4 do not seem to affect the secondary structure of T. brucei SL RNA, as shown by chemical modification of undermethylated SL RNA probed in vivo.
D000447:Aldehydes / Q000494:pharmacology; D000818:Animals; D001483:Base Sequence; D002074:Butanones; D003028:CME-Carbodiimide / Q000031:analogs & derivatives / Q000494:pharmacology; D002463:Cell Membrane Permeability; D003432:Cross-Linking Reagents; D008745:Methylation; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012315:RNA Caps / Q000378:metabolism; D012322:RNA Precursors / Q000737:chemistry*; D012326:RNA Splicing; D016053:RNA, Protozoan / Q000737:chemistry*; D014346:Trypanosoma brucei brucei / Q000235:genetics; D014351:Trypanosomatina / Q000737:chemistry* / Q000235:genetics
D000447:Aldehydes; D002074:Butanones; D003432:Cross-Linking Reagents; D012315:RNA Caps; D012322:RNA Precursors; D016053:RNA, Protozoan; C060331:1-cyclohexyl-3-(2-(4-morpholinyl)ethyl)carbodiimide; D003028:CME-Carbodiimide; C005135:kethoxal