title: Enzymatic and immunological activity of 4000 years aged bone alkaline phosphatase.
abstract: Structurally intact and functionally active human bone alkaline phosphatase was isolated from clavicle fragments of IDU, an Egyptian mummy of the Old Kingdom (2150 +/- 50 BC). Both anion exchange and affinity chromatographies were employed to optimise the preparation of the ancient enzyme resulting in a specific activity of 180 +/- 30 mU/mg. The intactness of the bone enzyme fractions of the wheat-germ lectin affinity chromatography was successfully demonstrated in an ELISA using the monoclonal antibody BAP A. Fortunately, the mummified bone was not contaminated by fungi or bacteria.
mesh_terms: D000469:Alkaline Phosphatase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D001842:Bone and Bones / Q000201:enzymology*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D002968:Clavicle; D004534:Egypt; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007527:Isoenzymes / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D009106:Mummies; D015032:Zinc / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D000469:Alkaline Phosphatase; D015032:Zinc