Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C.
The gene coding for opine dehydrogenase from Arthrobacter sp. strain 1C was cloned onto plasmid pBluescript KS(-), and the nucleotide sequence of the 1,077-bp open reading frame consisting of 359 codons was identified as the odh gene. Transformed Escherichia coli cells overproduced NAD+-dependent opine dehydrogenase under control of the promoter of the lac gene on pBluescript KS(-).
D016960:Agrobacterium tumefaciens / Q000201:enzymology / Q000235:genetics; D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003605:D-Amino-Acid Oxidase / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007763:Lac Operon; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000235:genetics; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D003605:D-Amino-Acid Oxidase; D000587:Oxidoreductases Acting on CH-NH Group Donors; C060511:opine dehydrogenase, NAD+; C021523:D-lysopine dehydrogenase