Detection and monitoring of a concomitant atypical myeloproliferative disorder and chronic lymphocytic leukemia by flow-cytometric immunophenotyping.
OBJECTIVE
To illustrate the utility of a broad panel of monoclonal antibodies to detect secondary processes or unexpected characteristics of the primary blood dyscrasia.


METHODS
Case report and discussion.


METHODS
Regional academic medical center.


METHODS
A 64-year-old man presenting with an apparent acute myeloid leukemia.


METHODS
Sequential immunophenotyping with a broad panel of monoclonal antibodies to monitor progression of disease and response to therapy.


METHODS
Identification and monitoring of the two atypical populations in this patient with correlation to the clinical status of the patient.


RESULTS
Identification of an unsuspected mature lymphoid clone and characterization of the evolution of the myelomonocytic clone.


CONCLUSIONS
The evolving mature lymphoid clone may have been overlooked in the context of a predominant atypical myeloproliferative process, particularly if a limited panel of monoclonal antibodies had been used for immunophenotyping. Sequential immunophenotyping was useful in monitoring the progression of each atypical process.
D015703:Antigens, CD / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis* / Q000473:pathology*; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009196:Myeloproliferative Disorders / Q000150:complications* / Q000175:diagnosis*; D013154:Spleen / Q000473:pathology
D015703:Antigens, CD