title: A method for producing microbe-free Amyloodinium ocellatum (Brown) with Percoll.
abstract: A method for collecting microbe-free Amyloodinium ocellatum from infected red drum (Sciaenops ocellatus) is described. Surface contamination of trophonts removed from fish by osmotic shock was reduced by washing the preparation with antibiotic containing saltwater and Percoll density gradient purification. The resulting tomont preparation was free of external contamination. Sterile, infective dinospores excysted from the tomonts after 72 h incubation at 26 degrees C in antibiotic containing 30 ppt saltwater.
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient / Q000379:methods; D056890:Eukaryota / Q000166:cytology / Q000254:growth & development / Q000302:isolation & purification*; D005393:Fish Diseases; D005399:Fishes / Q000469:parasitology*; D011205:Povidone; D011529:Protozoan Infections, Animal; D012623:Seawater; D012822:Silicon Dioxide
chemical_list: C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone