The purine-rich trinucleotide repeat sequences d(CAG)15 and d(GAC)15 form hairpins.
The structures of single-stranded (ss) oligonucleotides containing (CAG)15 [ss(CAG)15] or (GAC)15 [ss(GAC)15] were examined. At 10 degrees C, the electrophoretic mobilites of the two DNAs were similar to ss(CTG)15, a DNA that forms a hairpin containing base paired and/or stacked thymines. At 37 degrees C in 50 mM NaCl, single-strand-specific P1 nuclease cleaved the G33-G36 phosphodiesters of ss(GAC)15, and the G32-A34, G35-C36 phosphodiesters of ss(CAG)15 (where the loop apex of both DNAs = A34). Electrophoretic mobility melting profiles indicated that the melting temperature (Tm) of ss(CAG)15 in low (approximately 1 mM Na+) ionic strength was 38 degrees C. In contrast, the Tm of ss(GAC)15 was 49 degrees C, a value similar to the Tm of ss(CTG)15. These results provide evidence that ss(GAC)15 and ss(CAG)15 form similar, but distinguishable hairpin structures.
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D003839:Deoxyadenosines; D003849:Deoxyguanosine; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D015719:Single-Strand Specific DNA and RNA Endonucleases; D013696:Temperature; D018911:Trinucleotide Repeats
D003839:Deoxyadenosines; D009838:Oligodeoxyribonucleotides; D004247:DNA; D015719:Single-Strand Specific DNA and RNA Endonucleases; D003849:Deoxyguanosine