Comparison of agar-gel precipitin responses among strains of fowl adenovirus using antigens prepared from chorioallantoic membranes and chicken kidney cell cultures.
Agar-gel precipitin responses obtained for serologically different strains of fowl adenovirus (FAV) in tests using antigens prepared from FAV-infected chorioallantoic membranes (CAM antigen) and chicken kidney cell cultures (CKC antigen) were compared. Findings showed that both types of antigens exhibited less sensitivity to heterologous than to homologous antisera and that quantitative differences in sensitivity were present between serotypes. CAM antigens were more sensitive than CKC antigens to heterologous antisera. Polyvalent CAM antigens containing 2 or 3 antigens increased sensitivity in testing of field serum samples, resulting in a higher rate of detection.
D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000276:immunology; D000362:Agar; D000482:Allantois / Q000276:immunology; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D000259:Aviadenovirus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D002823:Chorion / Q000276:immunology; D003429:Cross Reactions; D007106:Immune Sera; D007668:Kidney / Q000276:immunology*; D011233:Precipitin Tests / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D012703:Serotyping
D000956:Antigens, Viral; D007106:Immune Sera; D000362:Agar