title: The cytosolic chaperonin subunit TRiC-P5 begins to be expressed at the two-cell stage in mouse embryos.
abstract: The cytosolic chaperonin TRiC is a large protein complex involved in the folding of newly synthesized actin and tubulin. The fertilization of the mouse oocyte is followed by a remodelling of the actin and tubulin filaments. The TRiC subunit TCP1 is expressed only from the 4-cell stage on, even though actin and tubulin are synthesized in the previous stages. We investigated the onset of synthesis of another subunit, TRiC-P5, during early mouse embryogenesis. We report that TRiC-P5 is synthesized at the 2-cell stage in an alpha-amanitin sensitive manner. Thus, it is expressed before TCP1 and is one of the first proteins to be synthesized after zygotic genome activation.
mesh_terms: D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002051:Burkitt Lymphoma; D002460:Cell Line; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins / Q000096:biosynthesis*; D003600:Cytosol / Q000378:metabolism; D005314:Embryonic and Fetal Development; D005260:Female; D005306:Fertilization; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D014176:Protein Biosynthesis; D011506:Proteins; D013480:Superovulation; D014407:Tumor Cells, Cultured; D015053:Zygote / Q000378:metabolism*
chemical_list: D000546:Amanitins; C533299:CCT3 protein, human; D046911:Macromolecular Substances; D011506:Proteins; C533304:TCP1 protein, human; C533315:Tcp1 protein, mouse; D056404:Chaperonin Containing TCP-1; D018833:Chaperonins