[Study on determination of gentamycin in serum by enhanced chemiluminesent immunoassay based on immobilized antibody and biotin-avidin system].
Gentamycin was determined by competition with biotin labeled gentamycin in the presence of an immobilized antibody to gentamycin. Microcrystalline cellulose was first coupled with sheep anti-rabbit antibody, then the anti-gentamycin antibody was conjugated and the competition reaction conducted. After separation, HRP-avidin was added to the precipitation and the amount of HRP conjugate determined by enhanced chemiluminescene. The limit of detection for gentamycin in serum was 63-78 ng/tube and the average recovery of gentamycin 103%. The correlation coefficient of the log-log regression equation. Magnitude of r was 0.991 +/- 0.006.
D000818:Animals; D001360:Avidin; D001710:Biotin; D005839:Gentamicins / Q000097:blood*; D007118:Immunoassay / Q000379:methods; D008163:Luminescent Measurements; D011817:Rabbits
D005839:Gentamicins; D001360:Avidin; D001710:Biotin