Development of the mouse B-cell repertoire.
The mouse B-cell repertoire early in ontogeny contains B cells with receptor immunoglobulins exhibiting high connectivity, multi/self-reactivity, and generally low affinity. This is due structurally to extensive restriction in the germline components used to generate the B-cell receptor. The selective pressure acting on the nascent repetoire has both negative and positive components as seen in our in vivo models. VH81X-bearing B cells from the VH81X transgenic mice (and probably from normal mice) are subject to self-selective pressure with two components: a positive one favoring a certain (self-reactive) specificity in the CD23-IgM+ population and a negative one preventing the entry of B cells with this specificity into the CD23+IgM+ compartment.
D000818:Animals; D000831:Animals, Newborn; D000916:Antibody Diversity; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D017637:Clonal Deletion; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D007107:Immune System / Q000196:embryology / Q000254:growth & development; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D051379:Mice / Q000235:genetics / Q000276:immunology*; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017385:Sequence Homology
D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region