Dolichyl phosphate, a potent inducer of apoptosis in rat glioma C6 cells.
Exposure of rat glioma C6 cells to dolichyl phosphate resulted in cell shrinkage followed by nuclear fragmentation and internucleosomal cleavage of genomic DNA, yielding ladder patterns of oligonucleosomal fragments, all characteristics of apoptosis. This phenomenon occurred in a dose and time dependent manner. Dolichol and prenol failed to induce apoptosis. The inhibitors of N-glycosylation, tunicamycin and swainsonine had no apparent effect on dolichyl phosphate-induced apoptosis. Apoptotic changes were also observed in HL-60 cells, SIRC cells and HeLa cells. Thus, dolichyl phosphate functions as a potential apoptosis inducer as well as an essential carrier lipid in the biosynthesis of N-linked glycoprotein.
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004247:DNA / Q000378:metabolism; D004288:Dolichol Phosphates / Q000494:pharmacology*; D004286:Dolichols / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006023:Glycoproteins / Q000037:antagonists & inhibitors; D006031:Glycosylation / Q000187:drug effects; D006367:HeLa Cells; D045782:Hemiterpenes; D006801:Humans; D007700:Kinetics; D000439:Pentanols / Q000494:pharmacology; D051381:Rats; D017026:Swainsonine / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014415:Tunicamycin / Q000494:pharmacology
D004288:Dolichol Phosphates; D004286:Dolichols; D006023:Glycoproteins; D045782:Hemiterpenes; D000439:Pentanols; D014415:Tunicamycin; C028306:dolichol monophosphate; C009034:prenol; D004247:DNA; D017026:Swainsonine