Bioassay of clindamycin in bile.
This study compares different preparations of standard solutions for determining clindamycin in bile. Pooled human serum, ox bile, 10% human bile, and 0.1 M phosphate buffer were compared, and pooled human bile was serving as reference standard curve. The use of pooled human serum and ox bile resulted in a considerable error of overestimation of clindamycin in bile. An error of underestimation occurred when 10% human bile or 0.1 M phosphate buffer was used as a standard solution. It is concluded that none of the different preparations tested are acceptable substitutes for human bile.
D000818:Animals; D001646:Bile / Q000032:analysis*; D001681:Biological Assay; D002417:Cattle; D002981:Clindamycin / Q000032:analysis* / Q000494:pharmacology; D006801:Humans; D008297:Male; D012505:Sarcina / Q000187:drug effects; D013045:Species Specificity
D002981:Clindamycin