Inhibitory activity of soluble material from sheep eosinophils for sheep complement.
Soluble material from frozen and thawed sheep eosinophils and sonicated extracts of eosinophils or their granules inhibited the alternative activation pathway dependent action of sheep complement for human erythrocytes sensitized by sheep antibody. Sephadex G-50 fractions of the sonicated eosinophil and granule extracts showed that inhibitory activity was associated with the void volume fraction, the fraction possessing the eosinophil peroxidase activity.
D000818:Animals; D001196:Ascariasis / Q000378:metabolism / Q000662:veterinary; D002850:Chromatography, Gel; D003169:Complement Inactivator Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000737:chemistry; D003911:Dextrans; D047209:Eosinophil Peroxidase; D004802:Eosinophilia / Q000378:metabolism; D004804:Eosinophils / Q000737:chemistry* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008321:Mammary Glands, Animal / Q000469:parasitology; D010544:Peroxidases / Q000302:isolation & purification / Q000494:pharmacology*; D012756:Sheep / Q000276:immunology*; D012757:Sheep Diseases / Q000378:metabolism / Q000469:parasitology; D012995:Solubility
D003169:Complement Inactivator Proteins; D003911:Dextrans; C025614:sephadex; D047209:Eosinophil Peroxidase; D010544:Peroxidases