Preparation of recombinant baculovirus by transfection of a ligated cDNA fragment without prior plasmid amplification in E. coli.
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D011088:DNA Ligases / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism*; D004274:DNA, Recombinant; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005784:Gene Amplification; D010957:Plasmids; D020365:RNA Helicases; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases; D018411:Spodoptera / Q000378:metabolism; D014162:Transfection; D017361:Viral Nonstructural Proteins / Q000235:genetics; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D011088:DNA Ligases; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial; D018076:DNA, Complementary; D004274:DNA, Recombinant; D015252:Deoxyribonucleases, Type II Site-Specific; D020365:RNA Helicases; D029701:Saccharomyces cerevisiae Proteins; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C037725:SCEI protein, S cerevisiae