An improved protocol for immunogold staining for scanning electron microscopy of cultured cells.
This paper describes an effective protocol for preparing immunogold-labelled cultured cells for high-resolution scanning electron microscopy. The cells examined were rat peripheral nerve Schwann cells and fibroblasts. The protocol employs low concentrations of chemicals and decreased fixation time. The resultant immunohistochemical cell labelling has little or no background staining, while maintaining the morphological integrity of the cell membrane.
D000818:Animals; D000831:Animals, Newborn; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D005347:Fibroblasts / Q000648:ultrastructure; D005727:Ganglia, Spinal / Q000166:cytology / Q000648:ultrastructure; D007150:Immunohistochemistry / Q000379:methods*; D007371:Interferon-gamma / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D016253:Microscopy, Immunoelectron / Q000379:methods*; D051381:Rats; D012583:Schwann Cells / Q000648:ultrastructure; D016707:Tissue Fixation
D007371:Interferon-gamma