Simulium damnosum s.l.: identification of inducible serine proteases following an Onchocerca infection by differential display reverse transcription PCR.
Vector-derived proteases are thought to be key to the regulation of filarial infections in Simulium damnosum s.I. To identify proteases of S. damnosum s.I. induced by infection with Onchocerca ochengi, a PCR-based differential display technique was used. By combining this method with homology-based serine protease primers transcripts can be detected from S. damnosum s.I. RNA.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry; D015971:Gene Expression Regulation, Enzymologic; D007303:Insect Vectors / Q000201:enzymology* / Q000235:genetics / Q000469:parasitology; D008969:Molecular Sequence Data; D009854:Onchocerca / Q000502:physiology*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D012843:Simuliidae / Q000201:enzymology* / Q000235:genetics / Q000469:parasitology; D014158:Transcription, Genetic
D017931:DNA Primers; D018076:DNA, Complementary; D012697:Serine Endopeptidases