title: Protective effect of verapamil on regional myocardial ischaemic injury in dog.
abstract: Ischaemic injury was produced in the dog heart by occluding the left anterior descending coronary artery just below the second diagonal branch for 3 h. Verapamil 5.0 mg/kg was injected intravenously 10 min before the occlusion. There was some decrease in ATP and creatine phosphate levels in the hearts of "no drug" animals as compared to sham-operated dogs. Verapamil significantly increase the level of these metabolites. The ATP/ADP ratio decreased from 8.5 +/- 0.60 in hearts from sham-operated dogs to 3.5 +/- 0.30 in the "no drug" group. Verapamil pretreatment resulted in values of the ATP/ADP ratio which were lower than, but not significantly different from, those observed in the control group. In ischaemic mitochondria the calcium content (Ca++) increased significantly as compared to the control and the Verapamil group. A significant increase in free fatty acid content in myocardial membrane preparations was also observed as compared to the sham-operated and the Verapamil group.
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000627:therapeutic use*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D004283:Dog Diseases / Q000517:prevention & control*; D004285:Dogs; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D007275:Injections, Intravenous / Q000662:veterinary; D008297:Male; D017202:Myocardial Ischemia / Q000517:prevention & control / Q000662:veterinary*; D009206:Myocardium / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D014700:Verapamil / Q000627:therapeutic use*
chemical_list: D002121:Calcium Channel Blockers; D005230:Fatty Acids, Nonesterified; D010743:Phospholipids; D014700:Verapamil