[Preparation of highly-purified RNA from yeasts using calcium ions].
The influence of temperature and Ca2+ ions at different concentrations on the effectiveness of RNA precipitation from water-salt extract of baker yeast was studied. Our findings allowed us to propose a method for producing highly purified RNA by precipitation with 180 mM Ca2+ ions from hot yeast extracts (95 degrees C) after removal of the protein fraction that could be also precipitated with 20 mM Ca2+ at 90 degrees C. The purity and yields of RNA samples obtained in such a manner was of 98% and 56% relative to theoretical values, respectively.
D002118:Calcium; D011232:Chemical Precipitation; D006358:Hot Temperature; D012331:RNA, Fungal / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D012331:RNA, Fungal; D002118:Calcium
Poluchenie vysokoochishchennoĭ RNK iz drozhzheĭ s pomoshch'iu ionov kal'tsiia.