Radical intermediates during degradation of lignin-model compounds and environmental pollutants: an electron spin resonance study.
1. I discuss the following aspects of Phanerochaete chrysosporium ligninase, the enzyme that has been shown to degrade a number of lignin-model compounds, and environmentally significant polycyclic aromatic hydrocarbons and polychlorinated phenols. 2. The primary mode of oxidation involves formation of a cation radical, which undergoes either hydrolysis or carbon-carbon bond cleavage. 3. In the presence of reducing substrate such as oxalic acid, ligninase has reductive activity that has been shown to be responsible for radical-mediated degradation of halogenated chemicals. 4. Electron spin resonance technique can be used to monitor the cation radical and other radical formation during ligninase-mediated degradation of lignin-model compounds and other chemicals.
D000818:Animals; D002412:Cations / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D004785:Environmental Pollutants / Q000032:analysis*; D005609:Free Radicals / Q000737:chemistry*; D006801:Humans; D008031:Lignin / Q000737:chemistry*; D008956:Models, Chemical
D002412:Cations; D004785:Environmental Pollutants; D005609:Free Radicals; D008031:Lignin