High-performance liquid chromatographic determination of prolylcarboxypeptidase activity in monkey kidney.
A simple HPLC procedure for the determination of prolylcarboxypeptidase activity in monkey kidney was established with Cbz-Pro-Ala used as substrate. Decrease of the substrate and increase of the product were stoichiometrically related to each other. Heat treatment at 60 degrees C freed the enzyme preparation of contaminating activities. Data on substrate specificity and influence of inhibitors suggested this method was sensitive for the determination of prolylcarboxypeptidase without the use of a radioactive substrate.
D000818:Animals; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006868:Hydrolysis; D007668:Kidney / Q000201:enzymology*; D008251:Macaca; D013379:Substrate Specificity
D002268:Carboxypeptidases; C022448:lysosomal Pro-X carboxypeptidase