Increased granzyme B mRNA after alloincompatible myoblast transplantation.
Normal C57BL/10SnJ myoblasts were transplanted into the tibialis anterior of C57BL/10SnJ, C57BL/ScSn mdx, or BALB/c mice. These transplantations allowed us to investigate the immune response not only against MHC but also against dystrophin introduced in the dystrophic muscles by such transplantations. Recently, our group reported following myoblast transplantations cellular infiltration of the host muscle by class II MHC cells, macrophages, and lymphocytes expressing CD4 or CD8 and IL-2 receptors. In the present study, activation of these infiltrating lymphocytes was investigated by measuring the expression of granzyme B mRNA. We used reverse-transcriptase polymerase chain reaction to detect granzyme B mRNA at various intervals after myoblast transplantations. To standardize the results, the mRNA were reverse transcribed using an oligo (dt) so that beta-actin mRNA could also be amplified from the same cDNA preparation. Granzyme B mRNA was increased for at least 3 weeks after MHC alloincompatible grafts. The absence of increased granzyme B expression after allocompatible transplantation in mdx mice suggests that dystrophin is not sufficiently immunogenic to induce short term acute rejection. These results indicate that lymphocytes infiltrated in muscles injected with histoincompatible myoblasts are activated and sustain the requirement for an adequate immunosuppression after such transplantations.
D000818:Animals; D001483:Base Sequence; D015704:CD4 Antigens / Q000032:analysis / Q000096:biosynthesis; D016827:CD8 Antigens / Q000032:analysis / Q000096:biosynthesis; D017931:DNA Primers; D015870:Gene Expression; D053804:Granzymes; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006650:Histocompatibility Testing; D006801:Humans; D007223:Infant; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology / Q000276:immunology / Q000637:transplantation*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D015375:Receptors, Interleukin-2 / Q000032:analysis / Q000096:biosynthesis; D012697:Serine Endopeptidases / Q000096:biosynthesis*; D014184:Transplantation, Homologous / Q000276:immunology / Q000502:physiology*
D015704:CD4 Antigens; D016827:CD8 Antigens; D017931:DNA Primers; D000949:Histocompatibility Antigens Class II; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2; C509213:GZMB protein, human; D053804:Granzymes; C509214:Gzmb protein, mouse; D012697:Serine Endopeptidases