Use of pulsed-field gel electrophoresis to link sporadic cases of invasive listeriosis with recalled chocolate milk.
Pulsed-field gel electrophoresis established the linkage between recalled chocolate milk and a multistate invasive listeriosis outbreak during a four-product recall period. Listeria monocytogenes isolates from four hospitalized patients and an environmental dairy sample displayed AscI restriction endonuclease digestion profiles identical to that of the chocolate milk isolate.
D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002099:Cacao; D002648:Child; D002675:Child, Preschool; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005260:Female; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics / Q000302:isolation & purification; D008088:Listeriosis / Q000453:epidemiology / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D008892:Milk / Q000382:microbiology*; D017720:Molecular Epidemiology; D014922:Wisconsin / Q000453:epidemiology
D004269:DNA, Bacterial