Genotyping of Bison bonasus kappa-casein gene following DNA sequence amplification.
DNA isolated from 30 Bison bonasus blood samples was used for amplification of a kappa-casein gene fragment to detect possible polymorphism. Bovine kappa-caseins exist in A, B and E variants. In the European bison from Poland, we have found only BB genotypes. Sequencing of the polymerase chain reaction product revealed further polymorphisms typical for B. bonasus.
D000818:Animals; D001483:Base Sequence; D016164:Bison / Q000235:genetics*; D002364:Caseins / Q000235:genetics*; D002417:Cattle / Q000235:genetics; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D005838:Genotype; D008969:Molecular Sequence Data; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012689:Sequence Homology, Nucleic Acid
D002364:Caseins; D017931:DNA Primers; D004247:DNA