The bovine herpesvirus 1 maturational proteinase and scaffold proteins can substitute for the homologous herpes simplex virus type 1 proteins in the formation of hybrid type B capsids.
We determined the nucleotide sequence of a 3.5-kb region of the bovine herpesvirus 1 (BHV-1) genome which contained the complete BHV-1 homologs of the herpes simplex virus type 1 (HSV-1) UL26 and UL26.5 genes. In HSV-1, the UL26 and UL26.5 open reading frames encode scaffold proteins upon which viral capsids are assembled. The UL26-encoded protein is also a proteinase and specifically cleaves both itself and the UL26.5-encoded protein. The overall BHV-1-encoded amino acid sequence showed only 41% identity to the HSV-1 sequences and was most divergent in the regions defined to be involved in the scaffolding function. We substituted the proteins encoded by the BHV-1 homologs of the UL26 and UL26.5 open reading frames, expressed in baculovirus, for the corresponding HSV-1 proteins in an in vitro HSV-1 capsid assembly system. The proteins expressed from the BHV-1 UL26 and UL26.5 homologs facilitated the formation of hybrid type B capsids indistinguishable from those formed entirely with HSV-1-encoded proteins.
D000595:Amino Acid Sequence; D000818:Animals; D002213:Capsid / Q000096:biosynthesis* / Q000648:ultrastructure; D002417:Cattle; D002460:Cell Line; D010450:Endopeptidases / Q000378:metabolism*; D005814:Genes, Viral; D016679:Genome, Viral; D007242:Herpesvirus 1, Bovine / Q000235:genetics* / Q000378:metabolism*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000378:metabolism; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D055503:Protein Multimerization; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D018139:Simplexvirus / Q000235:genetics* / Q000378:metabolism; D013045:Species Specificity; D018411:Spodoptera; D013379:Substrate Specificity; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
C054582:ICP35 protein, Human herpesvirus 1; D014764:Viral Proteins; C079287:scaffold protein, Herpes simplex virus-1; D010450:Endopeptidases; D012697:Serine Endopeptidases