A template for generation and comparison of three-dimensional selectin models.
We have complemented multiple sequence alignments of the lectin domains of the selectins with an analysis of structurally invariant regions in X-ray structures of the mannose-binding protein (MBP) and E-selectin. The analysis shows that regions of structural conservation between MBP and E-selectin extend beyond regions of rigorous sequence conservation within the selectin family and suggests that reliable three-dimensional models of selectins from different species can be generated by modification of only a few backbone segments in E-selectin. A model of the L-selectin lectin domain is built and discussed with regard to observed differences in selectin specificity.
D000595:Amino Acid Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D019040:E-Selectin / Q000737:chemistry*; D019041:L-Selectin / Q000737:chemistry; D037241:Mannose-Binding Lectins; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D016415:Sequence Alignment; D013698:Templates, Genetic
D002352:Carrier Proteins; D019040:E-Selectin; D037241:Mannose-Binding Lectins; D019041:L-Selectin