title: Distribution of membrane glycoproteins among the organelles of a single identified neuron of Aplysia. II. Isolation and characterization of a glycoprotein associated with vesicles.
abstract: Glycoprotein-I (mol.wt. 180,000) is associated with a vesicle fraction from the cytoplasm of R2, the giant cholinergic neuron in the abdominal ganglion. Electron microscopy has shown that R2 contains both lucent and compound vesicles. We have used anisomycin, an inhibitor of protein synthesis, to provide evidence that Component-I is a constituent of somatic compound vesicles. In the presence of the drug, [3H]N-acetylgalactosamine injected into R2 is incorporated almost exclusively into Component-I. Quantitative electron microscopic radioautography of treated cells shows a marked increase in the proportion of silver grains over compound vesicles and a decrease in labeling of other organelles compared with untreated cells. Analysis of Component-I, isolated using the chaotropic agent lithium diiodosalicylate, shows it to contain fucose, N-acetylgalactosamine, and N-acetylglucosamine. Proteolytic digestion with pronase yields a complex pattern of glycopeptides. The proportion of [3H]N-acetyl-galactosamine in these glycopeptides is altered in the presence of anisomycin. These results together with radioautographic analyses suggest that large carbohydrate chains are elaborated within the endoplasmic reticulum and that smaller chains are added in the Golgi region or on the membrane of the compound vesicle.
mesh_terms: D000116:Acetylgalactosamine / Q000378:metabolism; D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D001048:Aplysia; D001345:Autoradiography; D002799:Cholinergic Fibers / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005724:Ganglia / Q000187:drug effects / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008970:Molecular Weight; D009940:Organoids / Q000378:metabolism*; D013572:Synaptic Vesicles / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins; D000841:Anisomycin; D000116:Acetylgalactosamine