Incorporation of 5-iodo-2'-deoxyuridine and 5-bromo-2'-deoxyuridine into rodent DNA as determined by neutron activation analysis.
Using 5-iodo-2'-deoxyuridine (IdU) and 5-bromo-2'-deoxyuridine (BrdU) as DNA precursors, neutron activation analysis (NAA) of iodine and Br was developed as a quantitative method for determining DNA synthesis. Endogenous rodent tissue concentrations of bromine (Br) and iodine ranged 100-fold from a low of 0.06 microgram of iodine/g of rat gastrointestinal tract (GIT) to a high of 5.99 micrograms of Br/g of rat kidney. All 10 rodent tissues had concentrations of Br 4 to 76 times higher than those of iodine. Rat hepatic Br concentrations could be reduced 17-fold by dietary and pharmacological methods. Female Fischer 344 rats and male C57BL/6 mice were given 4-8 intraperitoneal injections of either IdU or BrdU as a DNA precursor. Tissue clearance of iodine in IdU-treated rodents was both faster and more complete (in mice 4 and in rats 17 h or less) than Br clearance from BrdU-treated rodents (at 162 h nonincorporated Br label still remains). In rat liver, lung, and kidney, the iodine label incorporated from IdU into DNA was stable for at least 162 h. The incorporation ratio is defined as the microgram halogen/tissue for either IdU- or BrdU-treated rodents divided by the microgram halogen/g tissue of untreated rodents. NAA-based studies of DNA synthesis gave high incorporation ratios in rat liver (5.3), rat lung (6.7), rat GIT (19.0), rat spleen (24.0), mouse GIT (10.1), and mouse spleen (25.8).
D000818:Animals; D001966:Bromine / Q000032:analysis; D001973:Bromodeoxyuridine / Q000032:analysis / Q000378:metabolism* / Q000493:pharmacokinetics; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D004064:Digestive System / Q000378:metabolism*; D005260:Female; D006219:Halogens / Q000032:analysis; D007065:Idoxuridine / Q000032:analysis / Q000378:metabolism* / Q000493:pharmacokinetics; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009501:Neutron Activation Analysis / Q000379:methods; D009928:Organ Specificity; D051381:Rats; D011916:Rats, Inbred F344; D013045:Species Specificity; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution
D006219:Halogens; D004247:DNA; D001973:Bromodeoxyuridine; D007065:Idoxuridine; D001966:Bromine