title: Characterization of the let-653 gene in Caenorhabditis elegans.
abstract: A mutation in the let-653 gene of Caenorhabditis elegans results in larval death. The lethal arrest is concurrent with the appearance of a vacuole anterior to the lower pharyngeal bulb. The position of the vacuole is consistent with a dysfunction of the secretory/excretory apparatus. Germline transformation rescue experiments were able to position the let-653 gene to two overlapping cosmid subclones. Sequence data generated from both cDNA and genomic DNA subclones indicated that let-653 encodes a mucin-like protein. Our characterization suggests that a mucin-like protein is essential for effective functioning of the secretory/excretory apparatus within C. elegans.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D029742:Caenorhabditis elegans Proteins; D003001:Cloning, Molecular; D015870:Gene Expression / Q000235:genetics; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000502:physiology; D007814:Larva / Q000166:cytology / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000502:physiology; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D012326:RNA Splicing / Q000235:genetics; D015183:Restriction Mapping; D017421:Sequence Analysis; D014170:Transformation, Genetic / Q000235:genetics
chemical_list: D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; C096862:LET-653 protein, C elegans; D009077:Mucins