Cleavage of double-stranded DNA by 'metalloporphyrin-linker-oligonucleotide' molecules: influence of the linker.
Manganese porphyrin-linker-triple-helix-forming oligonucleotide molecules were prepared and their ability to cleave in vitro a double-stranded DNA target present in the HIV-1 genome was studied. The nature of the linker is a determining factor of the cleavage efficiency. Cleavage yields as high as 80% were observed when the linker was a spermine residue and in the absence of a large excess of free spermine known to stabilize triplex structures. The hydrophobic nature of aliphatic diamine linker modified the cleaver-DNA interactions and reduced the efficiency of DNA cleavage.
D000998:Antiviral Agents; D001483:Base Sequence; D002413:Cations, Divalent; D004249:DNA Damage; D004279:DNA, Viral / Q000378:metabolism*; D004355:Drug Stability; D015497:HIV-1 / Q000235:genetics*; D006358:Hot Temperature; D008274:Magnesium / Q000494:pharmacology; D008665:Metalloporphyrins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry / Q000378:metabolism*; D012964:Sodium / Q000494:pharmacology; D013096:Spermine / Q000737:chemistry; D013329:Structure-Activity Relationship
D000998:Antiviral Agents; D002413:Cations, Divalent; D004279:DNA, Viral; D008665:Metalloporphyrins; D009841:Oligonucleotides; D013096:Spermine; D012964:Sodium; D008274:Magnesium