A mutation at the universally conserved position 529 in Escherichia coli 16S rRNA creates a functional but highly error prone ribosome.
A base substitution of G to U was constructed at position 529 in Escherichia coli 16S rRNA. The U529 mutant ribosomes were functional and present on polysomes but were highly error prone and caused a progressive loss of cell viability. They displayed elevated levels of readthrough of stop codons and frameshifting, and an increase in thermal sensitivity of beta-galactosidase, suggestive of missense errors. These results demonstrate that the university conserved G529 is involved in tRNA selection at the A site during protein synthesis.
D001483:Base Sequence; D017124:Conserved Sequence; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D006358:Hot Temperature; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D009690:Nucleic Acid Conformation; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D011489:Protein Denaturation; D012313:RNA; D012333:RNA, Messenger / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012270:Ribosomes / Q000378:metabolism*; D001616:beta-Galactosidase / Q000096:biosynthesis
D012333:RNA, Messenger; D012336:RNA, Ribosomal, 16S; C039919:RNA, recombinant; D012313:RNA; D001616:beta-Galactosidase