Determination of sulphonamides in human urine by azo dye precolumn derivatization and micellar liquid chromatography.
A high-performance liquid chromatographic method for the determination of sulphonamides in urine is reported. The drugs (sulphadiazine, sulphaguanidine, sulphamethizole, sulphamethoxazole, and sulphathiazole) were diazotized with nitrite and coupled with N-(1-naphthyl)ethylenediamine dihydrochloride in a sodium dodecyl sulphate (SDS) micellar medium. Separation of the sulphonamide azo dyes was performed on a C18 column with a 0.05 M SDS-2.4% pentanol mobile phase, which permitted the direct injection of the urine samples. The limits of detection were in the 0.1-0.3 micrograms/ml range.
D000890:Anti-Infective Agents / Q000652:urine*; D001391:Azo Compounds; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D008823:Micelles; D012016:Reference Values; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013449:Sulfonamides / Q000652:urine*
D000890:Anti-Infective Agents; D001391:Azo Compounds; D007202:Indicators and Reagents; D008823:Micelles; D013449:Sulfonamides