[Induced expression of whiG, a gene crucial for sporulation of Streptomyces coelicolor].
whiG gene has been subcloned into Streptomyces expression vector pAK203 containing inducible promoter tipA. The expression of whiG gene promoted the spore formation of S. coelicolor J1501 and recovered the sporulation ability of whiG-deficient S. coelicolor C71. Increased amount of whiG gene product was detected by Western blot hybridization after induction of thiostrepton. It will be helpful for the future study of in vitro transcription of whiG-dependent promoters.
D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D013171:Spores, Bacterial; D013302:Streptomyces / Q000235:genetics* / Q000502:physiology; D013883:Thiostrepton / Q000494:pharmacology
D013883:Thiostrepton