title: [Factors that affect analytical results in an enzyme immunoassay].
abstract: Factors that affect an enzyme immunoassay were reviewed from the practical point for clinical laboratory uses: these include prozone effect or hook effect, affinity and avidity, cross-reactivity and specificity of the antibody, and other non-specific factors such as pH, temperature, rheumatoid factor, complement, heterophile antibody and others. Precise mechanisms still remained unclear for these factors involved in non-specific reactions in the assay.
mesh_terms: D000818:Animals; D000915:Antibody Affinity; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D013696:Temperature