Elements of the polyomavirus replication origin required for homologous recombination mediated by large T antigen.
We introduced various elements of the polyomavirus origin of DNA replication into the genome of rat cells, and we analyzed their capacity to elicit rearrangements within the integrated sequences when exposed to large T antigen. The cis-acting sequences required for homologous recombination were those that make up a functional replication origin.
D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000502:physiology*; D015139:Blotting, Southern; D002460:Cell Line; D004252:DNA Mutational Analysis; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian; D005347:Fibroblasts; D015321:Gene Rearrangement; D016678:Genome; D016328:NF-kappa B / Q000378:metabolism; D011120:Polyomavirus / Q000235:genetics*; D051381:Rats; D011995:Recombination, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D018741:Replication Origin; D017384:Sequence Deletion; D016662:Virus Integration
D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral; D016328:NF-kappa B