SMI-32 antibody against non-phosphorylated neurofilaments identifies a subpopulation of cultured cortical neurons hypersensitive to kainate toxicity.
SMI-32, an antibody against a non-phosphorylated neurofilament epitope identifies a subpopulation of human cortical neurons preferentially lost in Alzheimer's or Huntington's disease. In murine cortical cultures SMI-32 labeled a small subset of neurons exhibiting enhanced vulnerability to kainate toxicity. Most SMI-32(+) neurons were GABAergic and exhibited kainate-activated Co2+ uptake. Thus expression of Ca2+ permeable AMPA or kainate receptor-gated channels likely underlies the heightened vulnerability of SMI-32(+) cortical neurons to kainate.
D000544:Alzheimer Disease / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D016923:Cell Death; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000276:immunology*; D006816:Huntington Disease / Q000276:immunology; D006967:Hypersensitivity; D007150:Immunohistochemistry; D007608:Kainic Acid / Q000633:toxicity*; D051379:Mice; D008815:Mice, Inbred Strains; D016900:Neurofilament Proteins / Q000276:immunology; D009474:Neurons / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D016900:Neurofilament Proteins; D007608:Kainic Acid