title: Bacteriophage P2: genes involved in baseplate assembly.
abstract: The sequences of two previously defined tail genes, V and J, of the temperate bacteriophage P2, and those of two new essential tail genes, W and I, were determined. Their order is the late gene promoter, VWJI, followed by the tail fiber genes H and G, and a transcription terminator. The V gene product is the small spike at the tip of the tail, and the J gene product lies at the edge of the baseplate. The W gene product may be homologous to the product of gene 25 of T4 phage, which is part of the T4 baseplate. A temperature-sensitive mutation in gene V affects satellite phage P4 production more than it affects the production of P2 helper phage. P4 mutations that partially compensate for this defect of gene V lie in the P4 capsid size determination gene, sid.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017901:Bacteriophage P2 / Q000235:genetics* / Q000502:physiology / Q000648:ultrastructure; D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000648:ultrastructure; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009154:Mutation; D011817:Rabbits; D011994:Recombinant Proteins; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics*; D019065:Virus Assembly / Q000502:physiology*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D011994:Recombinant Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins