title: Long-term effects of small bowel transplantation on intestinal motility.
abstract: We previously found that the contractile motility of the jejunum was increased 4 weeks after transplantation, and that the dominant intrinsic neural component was changed from cholinergic to nonadrenergic, noncholinergic (NANC). The present study investigated the long-term effects of transplantation on jejunal motility using rats that survived for 2 years after surgery. Jejunal strips were harvested from various groups of rats, and intestinal motility was assessed by electrical transmural stimulation. Stimulation produced a similar increase of contraction at 4 weeks and 2 years after grafting. Pretreatment with atropine showed that the cholinergic component of contraction was 45%, 24%, 32%, and 24% in controls, rats 4 weeks after transplantation, 2-year-old controls, and rats 2 years after transplantation, respectively. The NANC component (obtained with atropine and guanethidine) in each group was, respectively, 56%, 73%, 60%, and 69%. The actual value of the tetrodotoxin-insensitive myogenic component was significantly increased at 2 years after transplantation. A substance P antagonist ([Arg6, D-Trp7,9, Mephe8], substance P 6-11), inhibited most of the NANC contraction after transplantation. These results suggested that substance P has a key role in the motility of transplanted small bowel throughout the life of the grafts.
mesh_terms: D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D005769:Gastrointestinal Motility / Q000187:drug effects; D006145:Guanethidine / Q000494:pharmacology; D066298:In Vitro Techniques; D007583:Jejunum / Q000294:innervation / Q000502:physiology* / Q000637:transplantation*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000294:innervation / Q000502:physiology / Q000637:transplantation; D010446:Peptide Fragments / Q000494:pharmacology; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D051381:Rats; D011917:Rats, Inbred Lew; D012016:Reference Values; D013373:Substance P / Q000037:antagonists & inhibitors / Q000494:pharmacology; D013524:Surgical Flaps; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors; D014185:Transplantation, Isogeneic / Q000502:physiology*
chemical_list: D000700:Analgesics; D010446:Peptide Fragments; D013373:Substance P; D013779:Tetrodotoxin; C032292:substance P (6-11); D001285:Atropine; D011761:Pyrrolidonecarboxylic Acid; D006145:Guanethidine