Tissue preparation technique for microsphere assays of blood flow.
A simple method for preparing standardized tissue samples for microsphere assay of tissue blood flow is described. By reduction of tissue samples into a liquid state and centrifugation of the microspheres to the bottom of conical-shaped counting vials, the microspheres within all tissue and blood reference samples attain the same counting geometry. The validity and reproducibility of this technique has been established for planar-configured gamma-ray detectors in an in-situ porcine kidney model. A positive correlation coefficient of r = 0.99 was observed between the total kidney blood flow, as measured by a calibrated Transonic flow probe, and the microsphere reference sample technique.
D000818:Animals; D001783:Blood Flow Velocity; D002498:Centrifugation; D003037:Cobalt Radioisotopes; D007205:Indium Radioisotopes; D007668:Kidney / Q000098:blood supply*; D008433:Mathematics; D008863:Microspheres; D008954:Models, Biological; D015203:Reproducibility of Results; D012429:Ruthenium Radioisotopes; D013048:Specimen Handling / Q000379:methods*; D013326:Strontium Radioisotopes; D013552:Swine; D014004:Tin Radioisotopes
D003037:Cobalt Radioisotopes; D007205:Indium Radioisotopes; D012429:Ruthenium Radioisotopes; D013326:Strontium Radioisotopes; D014004:Tin Radioisotopes