Three weeks' and six months' exposure to aromatic white spirit affect synaptosomal neurochemistry in rats.
The effects of 3 weeks' or 6 months' inhalation exposure of rats to aromatic white spirit 6 h/day, 5 days/week at 0, 400, or 800 ppm were studied. Synaptosomal neurochemistry was investigated as index of the in situ conditions in the presynaptic nerve terminal. In both studies, the relative and absolute yield of synaptosomal protein were significantly reduced in the two exposed groups. Both studies demonstrated increased synaptosomal noradrenaline (NA), dopamine (DA), and 5-hydroxytryptamine (5-HT) concentrations, high- affinity 5-HT uptake rate and uptake capacity. It is hypothesized that a reduced density and total number of synapses in situ may be functionally compensated by increased NA, DA, and 5-HT neurotransmitter release, or by increased activity of corresponding neurons. The increased synaptosomal 5-HT uptake rates and uptake capacities may explain the previously demonstrated increased global and regional neurotransmitter concentrations and the present finding of increased synaptosomal 5-HT concentrations. These changes are interpreted as an indication of toxic effect on the CNS function and are considered supportive of recent findings of electrophysiological changes and affected motor activity following 6 months' exposure to dearomatized white spirit followed by an exposure-free period.
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002091:Butyrylcholinesterase / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D006838:Hydrocarbons / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism; D012997:Solvents / Q000008:administration & dosage / Q000633:toxicity*; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism*; D013997:Time Factors
D006838:Hydrocarbons; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; D012997:Solvents; D012701:Serotonin; C013089:ligroin; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D004298:Dopamine; D009638:Norepinephrine