title: Expression of an Arabidopsis potassium channel gene in guard cells.
abstract: The Arabidopsis thaliana KAT1 cDNA encodes a voltage-gated inward-rectifying K+ channel. A KAT1 genomic DNA clone was isolated and sequenced, and a 5' promoter and coding sequences containing eight introns were identified. Reporter gene analysis of transgenic plants containing the KAT1 promoter fused to bacterial beta-glucuronidase showed robust beta-glucuronidase activity primarily in guard cells.
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018744:DNA, Plant / Q000378:metabolism; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D015221:Potassium Channels / Q000096:biosynthesis* / Q000235:genetics; D024661:Potassium Channels, Inwardly Rectifying; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; D018744:DNA, Plant; C501210:KAT1 protein, Arabidopsis; D010940:Plant Proteins; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D011994:Recombinant Proteins