Chromosomal assignment of 20 cDNAs using flow-sorted spot-blot stamps.
Using a high-speed flow cytometer/sorter, we constructed spot-blot "stamps" measuring 3.5 x 2.0 cm containing 21 separate human chromosome fractions. Through hybridization to these stamps, 20 randomly selected cDNAs were assigned to specific chromosomes. Sequencing and BLAST database screening confirmed the location of one gene (UCHL1) and allowed the assignment of two other previously identified genes (LRP130 and cDNA IB871.)
D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary; D005434:Flow Cytometry; D006801:Humans; D008969:Molecular Sequence Data
D018076:DNA, Complementary