title: A point mutation in the VP4 coding sequence of coxsackievirus B4 influences virulence.
abstract: In analyzing the molecular determinants of virulence of coxsackievirus B4, chimeric viruses were constructed from avirulent and virulent viruses. The vCB424 recombinant contained a single nucleotide substitution on an avirulent genetic background, resulting in replacement of Ser-16 of VP4 with Arg-16. Mice infected with vCB424 displayed an intermediate phenotype.
mesh_terms: D000595:Amino Acid Sequence; D000681:Amylases / Q000097:blood; D000818:Animals; D001120:Arginine; D002213:Capsid / Q000235:genetics*; D036022:Capsid Proteins; D002678:Chimera; D003384:Coxsackievirus Infections / Q000473:pathology / Q000503:physiopathology / Q000821:virology*; D029822:Enterovirus B, Human / Q000235:genetics* / Q000472:pathogenicity* / Q000648:ultrastructure; D005838:Genotype; D051379:Mice; D010179:Pancreas / Q000473:pathology / Q000821:virology; D010641:Phenotype; D017354:Point Mutation; D015183:Restriction Mapping; D012694:Serine; D010948:Viral Plaque Assay; D014774:Virulence / Q000235:genetics
chemical_list: D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; D012694:Serine; D001120:Arginine; D000681:Amylases