title: Na channel density in extrajunctional sarcolemma of fast and slow twitch mouse skeletal muscle fibres: functional implications and plasticity after fast motoneuron transplantation on to a slow muscle.
abstract: Na channel densities were measured in fast and slow twitch mouse skeletal muscle fibres using the loose patch voltage clamp technique. It was found that Na channel density was approximately four times greater in fast twitch fibres than in slow. Computer simulations of action potential propagation in these fibres strongly suggest that the higher channel densities in fast twitch fibres are necessary to maintain action potential amplitude and fidelity of transmission across the neuromuscular junction, especially during the periods of rapid stimulation that these fibres are subjected to by their motoneurons. Transplantation of a foreign nerve containing axons which had previously innervated fast twitch fibres on to a slow twitch muscle resulted in an approximate doubling of the Na channel density in fibres innervated by the foreign nerve. These results suggest that motoneurons may exert considerable control over Na channel density in the muscle fibres they innervate.
mesh_terms: D000818:Animals; D003198:Computer Simulation; D051379:Mice; D009046:Motor Neurons / Q000637:transplantation*; D018656:Muscle Fibers, Fast-Twitch / Q000737:chemistry*; D018657:Muscle Fibers, Slow-Twitch / Q000737:chemistry* / Q000502:physiology; D018482:Muscle, Skeletal / Q000294:innervation / Q000648:ultrastructure; D009469:Neuromuscular Junction / Q000737:chemistry / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology*; D018408:Patch-Clamp Techniques; D012508:Sarcolemma / Q000737:chemistry*; D015222:Sodium Channels / Q000032:analysis* / Q000502:physiology; D016042:Transplantation, Heterotopic
chemical_list: D015222:Sodium Channels