Enzyme inhibition during the conversion of squalene to cholesterol.
Two separate enzymatic assays were developed in order to test the selectivity of inhibitors in cholesterol biosynthesis. One assay detects inhibition of delta 5.7-sterol delta 7-reductase, the enzyme involved in the conversion of 7-dehydrocholesterol to cholesterol. Delta 5.7-Sterol delta 7-reductase was inhibited by both RPR 101821, a protonated cyclohexylamine, and BM 15.766, a piperazine derivative, with IC50 values of 1 microM. The second assay detects accumulation of any of five intermediates (squalene oxide, squalene dioxide, lanosterol, desmosterol, and 7-dehydrocholesterol) upon inhibition of enzymes catalyzing reactions in the conversion of squalene to cholesterol. In this assay, inhibition data were most accurate when control assays exhibited a conversion of squalene to cholesterol in the order of 50%. The time required to attain 50% conversion of squalene to cholesterol was 6 h. Given a high inhibitor to substrate concentration ratio and the possible values of Ki, kon, and koff for the reaction between enzymes and inhibitor to form enzyme-inhibitor complexes, it was predicted that in the presence of inhibitors, intermediate accumulation could still be observed after 6 h incubation. The experimental results were in agreement with this prediction.
D000818:Animals; D001583:Benzoxazoles / Q000494:pharmacology; D002784:Cholesterol / Q000378:metabolism*; D003514:Cyclohexylamines / Q000494:pharmacology; D003684:Dehydrocholesterols / Q000302:isolation & purification / Q000378:metabolism; D003897:Desmosterol / Q000302:isolation & purification / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000037:antagonists & inhibitors*; D007810:Lanosterol / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D010088:Oxidoreductases / Q000037:antagonists & inhibitors; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010879:Piperazines / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013185:Squalene / Q000302:isolation & purification / Q000378:metabolism*
D001583:Benzoxazoles; D003514:Cyclohexylamines; D003684:Dehydrocholesterols; D004791:Enzyme Inhibitors; D010879:Piperazines; C096297:RPR 101821; C033144:lathosterol delta-5-dehydrogenase; D007810:Lanosterol; D003897:Desmosterol; D013185:Squalene; C047457:BM 15766; D002784:Cholesterol; C016705:7-dehydrocholesterol; D010088:Oxidoreductases; D044925:Oxidoreductases Acting on CH-CH Group Donors; D013186:Farnesyl-Diphosphate Farnesyltransferase