title: Gas phase infrared spectra of 209 polychlorinated biphenyl congeners using gas chromatography with Fourier transform infrared detection: internal standardization with a 13C-labelled congener.
abstract: The gas phase infrared spectra of biphenyl and 209 polychlorinated biphenyls (PCB) are observed; 30 are presented in graphical form so that their uniqueness can be appreciated. The spectra were observed with a gas chromatograph linked via a light pipe with a Fourier transform infrared spectrometer. Unequivocal library matches (with match factors > 990 in 1000) can be performed with 50 ng of a congener injected into the chromatograph. Quantitation with the technique was briefly investigated with 2,2',5,5'-tetrachlorobiphenyl (BZ no. 52). Selective wavelength chromatograms of the most intense absorption (1457cm-1) were integrated. Septuplicate injections showed that 20 ng of the compound could be determined with +/- 4% precision. The resolution of the spectrometer was sufficient to distinguish between the 13C-labelled trichlorobiphenyl and the unlabelled material, indicating the possibility of internal or surrogate standardization of PCB analysis.
mesh_terms: D002247:Carbon Isotopes; D002849:Chromatography, Gas; D007553:Isotope Labeling; D011078:Polychlorinated Biphenyls / Q000032:analysis*; D012015:Reference Standards; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D002247:Carbon Isotopes; D011078:Polychlorinated Biphenyls