Production, purification and characterization of two proteinaceous hen-egg-white lysozyme inhibitors from Pseudomonas aeruginosa M-1001.
Two proteinaceous lysozyme inhibitors, hen-egg-white lysozyme inhibitors F-I and F-II, were isolated from the culture broth of a bacterial strain identified as Pseudomonas aeruginosa M-1001. Maximum lysozyme inhibitory activity was obtained when the bacterium was grown aerobically in a medium consisting of 0.25% glucose, 0.25% beef extract, 0.25% polypepton, 1.0% sodium L-glutamate, and 1.0% soluble starch (pH 7.0) at 37 degrees C after 20-24 hrs. F-I and F-II were purified 20 and 7.5-fold, respectively, from the culture supernatant of P. aeruginosa M-1001 by ammonium sulfate fractionation, DEAE-Sepharose CL-6B column chromatography, and Sephacryl S-200 gel chromatography. The molecular weights of F-I and F-II were estimated to be about 57,000 and 33,000, by SDS-PAGE, respectively. F-I was stable in a pH range between 6 and 10 and below 50 degrees C. F-II was stable in a pH range between 6 and 11 and below 40 degrees C. Many Gram-positive bacteria were found to be inhibited by the crude lysozyme inhibitors.
D000645:Ammonium Sulfate; D011232:Chemical Precipitation; D002845:Chromatography; D004355:Drug Stability; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009113:Muramidase / Q000037:antagonists & inhibitors*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature
D004791:Enzyme Inhibitors; D009113:Muramidase; D000645:Ammonium Sulfate