Application of DNA fingerprinting with digoxigenated oligonucleotide probe (CAC)5 to analysis of the genetic variation within Taenia taeniaeformis.
DNA from T. taeniaeformis digested with the restriction endonuclease was hybridized with digoxigenated oligonucleotide probe (CAC)5. Metacestode and adult showed same clear multibanding patterns, which were characteristic of multilocus DNA fingerprinting. The fingerprinting patterns were quite different from those of the rodent hosts. Genetic variations in 4 laboratory-reared isolates of T. taeniaeformis, including 3 isolates which have been reported to be indistinguishable by infectivity, morphology and protein composition of metacestode, were investigated using this technique. Each of the 4 isolates exhibited isolate-specific fingerprinting patterns and were easily distinguished from one another, thus it was considered that (CAC)5 was a highly resolvable and informative probe for cestodes. However, it was also indicated that (CAC)5 was so sensitive that applying fingerprinting with (CAC)5 to taxonomical or phylogenetic analysis was limited where habitat of the host was restricted to the small area. In comparison to fingerprinting with 32P-labeled (CAC)5, fingerprinting with digoxigenated (CAC)5 represented more and sharper bands. It was considered that a digoxigenated probe was more useful for genetic analysis of cestodes.
D000818:Animals; D003411:Arvicolinae / Q000469:parasitology; D001483:Base Sequence; D016172:DNA Fingerprinting; D018509:DNA, Helminth / Q000737:chemistry*; D004076:Digoxigenin; D004587:Electrophoresis, Agar Gel; D014644:Genetic Variation; D051379:Mice / Q000469:parasitology; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D051381:Rats / Q000469:parasitology; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D013621:Taenia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
D018509:DNA, Helminth; D015345:Oligonucleotide Probes; D004076:Digoxigenin