Sample preparation of human tumors prior to two-dimensional electrophoresis of proteins.
Sample preparation procedures are critical for obtaining high quality two-dimensional electrophoresis (2-DE) separations from clinical tumor materials. In this communication, we describe procedures which we have found satisfactory for preparing various tumor samples for 2-DE. By these procedures, tumor cells are enriched from serum proteins and contaminating stromal cells. Tumors can be prepared with good reproducibility to obtain high resolution using these techniques.
D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D013048:Specimen Handling / Q000379:methods*
D009363:Neoplasm Proteins