Fibronectin peptide DRVPHSRNSIT and fibronectin receptor peptide DLYYLMDL arrest gastrulation of Rana pipiens.
Gastrulation is characterized by dramatic cell migration which is thought to require the interaction of cell adhesion molecules with extracellular molecules. We have tested two novel peptides, a fibronectin peptide and a fibronectin receptor peptide, for their effects on gastrulation of the leopard frog Rana pipiens. The fibronectin peptide DRVPHSRNSIT corresponds to residues 1373-1383 of the cell-binding domain of fibronectin; the receptor peptide DLYYLMDL corresponds to residues 124-131 of beta 1 subunit of a variety of integrins including alpha 5 beta 1. Either of these peptides significantly inhibited gastrulation after being microinjected into mid-blastulae. These results indicate that these sequences may correspond to the ligand/receptor interaction sites of fibronectin and its receptor(s).
D000595:Amino Acid Sequence; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005353:Fibronectins / Q000737:chemistry / Q000494:pharmacology*; D005775:Gastrula / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008845:Microinjections; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*; D011894:Rana pipiens; D017469:Receptors, Fibronectin / Q000737:chemistry; D013329:Structure-Activity Relationship
D005353:Fibronectins; D010446:Peptide Fragments; D017469:Receptors, Fibronectin