Green fluorescent protein production in the cellular slime molds Polysphondylium pallidum and Dictyostelium discoideum.
The green fluorescent protein-encoding gene from Aequorea victoria has been cloned into several different transforming vectors and expressed in the cellular slime molds, Polysphondylium pallidum and Dictyostelium discoideum. We find that the protein is stable and non-toxic in both species, can be easily visualized in living and fixed specimens, and can be used to purify rare cells by fluorescence-activated cell sorting (FACS).
D000818:Animals; D001483:Base Sequence; D004023:Dictyostelium / Q000235:genetics* / Q000378:metabolism; D005434:Flow Cytometry; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins