Inhibition of glycosphingolipid synthesis induces p34cdc2 activation in Xenopus oocyte.
In Xenopus prophase-blocked oocytes, it is assumed that progesterone interacts with the plasma membrane to initiate a signalling cascade that ultimately leads to MPF activation. Progesterone regulates negatively the cAMP pathway through an inhibition of adenylate cyclase. However, the mechanisms linking the initial action of the hormone with adenylate cyclase activity remain to be elucidated. Here, we demonstrate that PDMP, an inhibitor of glucosphingolipid synthesis, triggers oocyte meiotic maturation in a cAMP- and cycloheximide-dependent manner, whereas exogenous ceramide is unefficient. We propose that sphingolipid metabolism and targeting represent an important regulatory process of oocyte meiosis.
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D002772:Cholera Toxin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005260:Female; D006028:Glycosphingolipids / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007700:Kinetics; D009025:Morpholines / Q000494:pharmacology*; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D011374:Progesterone / Q000494:pharmacology; D011418:Prophase; D015398:Signal Transduction / Q000187:drug effects; D014982:Xenopus laevis
D004791:Enzyme Inhibitors; D006028:Glycosphingolipids; D009025:Morpholines; D011374:Progesterone; C033110:RV 538; D002772:Cholera Toxin; D003513:Cycloheximide; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D016203:CDC2 Protein Kinase; D015056:1-Methyl-3-isobutylxanthine