The mechanism of interaction of sodium dodecyl sulfate with elastic fibers.
Sodium dodecyl sulfate (SDS), an anionic hydrophobic ligand, is known to alter the mechanical properties of elastic fibers. In order to analyze the mechanism of the alteration, two forms of fibrous elastins, "solid" and "powder" elastins, which consisted of fascicular elastic fibers and single or oligomeric elastic fibers, respectively, were prepared from bovine aorta, and the interactions of SDS with these elastins in the presence and absence of 0.15 M NaCl were studied. The solid elastin was able to retain 1.2- to 1.4-fold larger amounts of SDS than the powder elastin under both conditions, and both elastins retained 1.2-fold or larger amounts of SDS in the presence of NaCl than in its absence. Whereas both elastins released the retained SDS gradually on repeated washing with an SDS-free buffer, the release rates from the solid elastin, especially the rate in the presence of NaCl, were much smaller than those from the powder elastin, and the solid elastin retained approximately 40% of the bound SDS under conditions where the powder elastin lost most of its SDS. The SDS-binding capacities of both elastins were significantly lower than those of soluble kappa-elastin and serum albumin, which bound SDS homogeneously on the polypeptide chains. When the washed SDS-bound solid elastin was incubated with methylene blue and examined under a microscope, most of the methylene blue-SDS complex was located at the interfiber spaces of the elastic fibers. These results suggest that SDS alters the mechanical properties of elastic fibers by binding to the interfiber spaces and surfaces of the fibers rather than by binding to the internal polypeptide chains.
D000818:Animals; D001011:Aorta / Q000737:chemistry; D001665:Binding Sites; D002417:Cattle; D004396:Coloring Agents / Q000378:metabolism; D003956:Dialysis; D004547:Elastic Tissue / Q000737:chemistry; D004549:Elastin / Q000737:chemistry* / Q000378:metabolism; D008751:Methylene Blue / Q000737:chemistry; D008853:Microscopy; D010455:Peptides / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry* / Q000378:metabolism
D004396:Coloring Agents; D010455:Peptides; D012967:Sodium Dodecyl Sulfate; D012965:Sodium Chloride; D004549:Elastin; D008751:Methylene Blue