An E2F-dependent late-serum-response promoter in a gene that controls glycolysis.
The F-type mRNA of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is found in proliferating, but not in quiescent, cells. This bifunctional enzyme catalyses the synthesis and degradation of fructose-2,6-bisphosphate, a potent stimulator of glycolysis. F-type mRNA concentration decreased upon differentiation of rat rhabdomyosarcoma cells; it increased in Rat-1 fibroblasts stimulated to proliferate by serum, by epidermal growth factor, or by the v-src oncogene product. This increase resulted, at least in part, from a stimulation of F promoter activity. The stimulation occurred at the G1/S transition of the cell cycle. It depended on a binding site for the oncogenic transcription factor E2F located in the first exon of F-type mRNA. This effect was inhibited by agents that increase cAMP concentration. The data provide the first evidence that a gene involved in the control of glycolysis can be regulated by a late-serum-response promoter in an E2F-dependent way.
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins; D002453:Cell Cycle; D018797:Cell Cycle Proteins; D000242:Cyclic AMP / Q000502:physiology; D004268:DNA-Binding Proteins / Q000378:metabolism; D050684:E2F Transcription Factors; D006597:Fructose-Bisphosphatase / Q000235:genetics*; D005786:Gene Expression Regulation; D006019:Glycolysis; D008934:Mitogens / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D015688:Oncogene Protein pp60(v-src) / Q000502:physiology; D010732:Phosphofructokinase-1 / Q000235:genetics*; D025481:Phosphofructokinase-2; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D056465:Retinoblastoma-Binding Protein 1; D050680:Transcription Factor DP1; D014157:Transcription Factors / Q000502:physiology*; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D008934:Mitogens; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D056465:Retinoblastoma-Binding Protein 1; D050680:Transcription Factor DP1; D014157:Transcription Factors; D000242:Cyclic AMP; D025481:Phosphofructokinase-2; D010732:Phosphofructokinase-1; D015688:Oncogene Protein pp60(v-src); D006597:Fructose-Bisphosphatase