Immunization with the paraneoplastic encephalomyelitis antigen HuD does not cause neurologic disease in mice.
Paraneoplastic encephalomyelitis/paraneoplastic sensory neuronopathy (PEM/PSN) associated with small cell lung cancer is characterized by high serum and CSF titers of anti-neuronal (anti-Hu) antibodies and by intrathecal synthesis of anti-Hu IgG. A pathologic role for the anti-Hu antibodies in PEM/PSN is further suggested by reported intraneuronal accumulation of the antibodies in the nervous system of PEM/PSN patients at autopsy. We immunized SJL/J mice, Lewis rats, and Hartley guinea pigs with purified recombinant HuD fusion protein. In spite of high-titer anti-HuD antibodies, neurologic and pathologic examination of the animals was normal. Apparent uptake of purified IgG by neurons in the brain proved to be artifactual.
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage; D000917:Antibody Formation; D001483:Base Sequence; D001921:Brain / Q000473:pathology; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; D004679:Encephalomyelitis / Q000276:immunology* / Q000473:pathology; D006168:Guinea Pigs; D006801:Humans; D007114:Immunization; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D010257:Paraneoplastic Syndromes / Q000276:immunology* / Q000473:pathology; D016601:RNA-Binding Proteins / Q000008:administration & dosage / Q000276:immunology*; D051381:Rats
D000906:Antibodies; D051959:ELAV Proteins; D000067779:ELAV-Like Protein 4; C498010:ELAVL4 protein, human; C498011:Elavl4 protein, mouse; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins