[Cytocidal effect of HH07A, a derivative of hainanensine, on L1210 cells in vitro].
The L1210 cells rapidly ceased to proliferate and its mitotic index decreased markedly after being exposed to HH07A 2 micrograms . ml-1 during exponential growth phase. However, 34.6% of the cells were still able to form colonies in soft agar if HH07A was removed after 24 h of incubation (the colony-forming efficiency for control cells was 63.7%) and the cell viability remained at about 94%. The DNA contents in L1210 cells were measured with a flow-cytometer. The results showed that the cell-cycle was blocked at the S phase and the number of cells in G2 + M phase decreased significantly. The time response course for L1210 cells indicated that the inhibitory effect of HH07A on L1210 clonogenic cells was slightly time dependent.
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004273:DNA, Neoplasm / Q000378:metabolism; D006248:Harringtonines / Q000494:pharmacology*; D007939:Leukemia L1210 / Q000473:pathology*; D051379:Mice; D008940:Mitotic Index / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014410:Tumor Stem Cell Assay
D000972:Antineoplastic Agents, Phytogenic; D004273:DNA, Neoplasm; C095422:HH07A; D006248:Harringtonines