Photolysis of a newly synthesized caged glycine activates the glycine receptor of rat CNS neurons.
The functions of 12 different newly synthesized caged glycines were examined on strychnine-sensitive glycine receptors on rat hypothalamic neurons using photochemical and physiological methods. Quantitative analysis of Cl- current induced by photolyzed glycines revealed N-[1-(2-nitrophenyl)ethylcarbonoxyl] glycine (N4-caged glycine) to be the most effectively photolyzed. The current amplitude induced by photolyzed N4-caged glycine was almost the same as that induced by normal glycine. We propose N-[1-(2-nitrophenyl)ethylcarbonoxyl] to be a useful photolabile moiety for new caged neurotransmitters.
D000818:Animals; D018118:Chloride Channels / Q000187:drug effects / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005998:Glycine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D007700:Kinetics; D009474:Neurons / Q000187:drug effects*; D010782:Photolysis; D051381:Rats; D018009:Receptors, Glycine / Q000819:agonists*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014697:Ventromedial Hypothalamic Nucleus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D018118:Chloride Channels; C096303:N-(1-(2-nitrophenyl)ethylcarbonoxyl)glycine; D018009:Receptors, Glycine; D005998:Glycine