Development of a melanin-based high-performance liquid chromatography stationary phase and its use in the study of drug-melanin binding interactions.
Drug-melanin interactions were studied using a melanin-based HPLC column. Two approaches were chosen for the preparation of the stationary phases: covalent coupling of synthetic L-dopa melanin and in situ polymerization of L-dopa. Retention of a series of phenothiazines on melanin-based stationary phases was attributed to binding to melanin. Frontal affinity chromatography experiments on one melanin-based column allowed us to calculate the affinity and binding capacity of chlorpromazine and promethazine. A competition was observed between chlorpromazine and haloperidol which was qualitatively consistent with previously described results. Data indicated that the interaction was not a simple competition at one site.
D014150:Antipsychotic Agents / Q000737:chemistry* / Q000302:isolation & purification*; D001667:Binding, Competitive; D002746:Chlorpromazine / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006220:Haloperidol / Q000302:isolation & purification; D007700:Kinetics; D007980:Levodopa / Q000737:chemistry; D008433:Mathematics; D008543:Melanins / Q000737:chemistry; D010640:Phenothiazines / Q000737:chemistry* / Q000302:isolation & purification*; D011398:Promethazine / Q000302:isolation & purification
D014150:Antipsychotic Agents; D008543:Melanins; D010640:Phenothiazines; D007980:Levodopa; D011398:Promethazine; D006220:Haloperidol; D002746:Chlorpromazine