Cyclodextrins for growth of Helicobacter pylori and production of vacuolating cytotoxin.
Growth of Helicobacter pylori in liquid culture requires the addition of media supplements that often interfere with subsequent purification of bacterial antigens. In order to determine whether cyclodextrins can substitute for conventional H. pylori growth supplements, we cultured H. pylori in the presence of five commercially available cyclodextrins. The effect of these compounds on the production of the vacuolating cytotoxin antigen was evaluated. Several cyclodextrins supported flourishing growth and permitted the consistent production of vacuolating cytotoxin. These data suggest that Brucella broth supplemented with cyclodextrins is an improved medium for bacterial culture and industrial production of H. pylori antigens.
D000942:Antigens, Bacterial / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis*; D003470:Culture Media; D003505:Cyclodextrins / Q000494:pharmacology*; D003603:Cytotoxins / Q000096:biosynthesis*; D016480:Helicobacter pylori / Q000254:growth & development* / Q000378:metabolism; D007218:Industrial Microbiology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; D003505:Cyclodextrins; D003603:Cytotoxins; C074689:VacA protein, Helicobacter pylori