Effect of proteolytic enzymes on the lysis and growth of oral bacteria.
Previous studies have shown increased levels of proteolytic enzymes in affected periodontal sites. The aim of the present investigation was to evaluate the effect of proteolytic environments on the lysis and growth of selected oral bacteria associated with either healthy or diseased periodontal sites. The effect of trypsin, chymotrypsin and proteinase K on cell lysis was determined following incubation with bacteria, whereas the effect of the same proteolytic enzymes on bacterial growth was tested using a disc-plate technique. Overall, gram-positive bacteria appeared to be more resistant to lysis than gram-negative bacteria. The most susceptible bacteria were Actinomyces spp., Eubacterium saburreum, Prevotella intermedia, Capnocytophaga ochracea, Fusobacterium nucleatum, Prevotella loescheii, Treponema denticola and Actinobacillus actinomycetemcomitans. The disc-plate procedure indicated that the growth of Actinomyces spp., E. saburreum, C. ochracea, P. intermedia, P. loescheii, Porphyromonas gingivalis and T. denticola were the most affected, more particularly by chymotrypsin and proteinase K. Interestingly, the growth of F. nucleatum was rather stimulated by proteolytic enzymes. The observations reported in this investigation indicate that specific and general proteolytic activities have the ability to lyse some oral bacterial species and to interfere with their growth. It is suggested that such effects could represent new mechanisms by which the bacterial ecology of subgingival sites may be affected.
D000190:Actinomyces / Q000187:drug effects / Q000254:growth & development; D016976:Aggregatibacter actinomycetemcomitans / Q000187:drug effects / Q000254:growth & development; D000818:Animals; D001433:Bacteriolysis / Q000187:drug effects*; D002167:Campylobacter / Q000187:drug effects / Q000254:growth & development; D002206:Capnocytophaga / Q000187:drug effects / Q000254:growth & development; D002417:Cattle; D002918:Chymotrypsin / Q000378:metabolism / Q000494:pharmacology; D017753:Ecosystem; D019286:Endopeptidase K; D005051:Eubacterium / Q000187:drug effects / Q000254:growth & development; D016967:Fusobacterium nucleatum / Q000187:drug effects / Q000254:growth & development; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000187:drug effects* / Q000254:growth & development; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D010519:Periodontium / Q000201:enzymology / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development; D018720:Prevotella / Q000187:drug effects / Q000254:growth & development; D012697:Serine Endopeptidases / Q000378:metabolism / Q000494:pharmacology*; D013291:Streptococcus / Q000187:drug effects / Q000254:growth & development; D013559:Symbiosis; D013997:Time Factors; D014208:Treponema / Q000187:drug effects / Q000254:growth & development; D014357:Trypsin / Q000378:metabolism / Q000494:pharmacology; D014678:Veillonella / Q000187:drug effects / Q000254:growth & development
D012697:Serine Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin; D019286:Endopeptidase K