Inhibitory effect of curcumin on SOS functions induced by UV irradiation.
The antigenotoxic effects of curcumin, including the inhibition of SOS induction and mutagenesis by UV light, were investigated in Salmonella typhimurium TA1535/pSK1002 and Escherichia coli K-12 strains. Induction of the SOS gene (umuC) expression was assayed by measuring accumulated beta-galactosidase activity. We found that curcumin blocked umuC induction promoted by UV irradiation in a dose-dependent manner. Also, with another SOS response, Weigle reactivation, we observed that curcumin effectively inhibited phage reactivation by UV irradiation. Furthermore, we tested the effect of curcumin on UV mutagenesis. We showed that mutagenesis induced by UV irradiation was suppressed by the addition of curcumin. Together these results indicate that curcumin acts as an inhibitor of SOS functions including UV mutagenesis.
D016587:Antimutagenic Agents / Q000494:pharmacology*; D010582:Bacteriophage lambda / Q000187:drug effects; D004396:Coloring Agents / Q000494:pharmacology*; D003474:Curcumin / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects; D013014:SOS Response, Genetics / Q000187:drug effects* / Q000235:genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects; D014466:Ultraviolet Rays; D014775:Virus Activation / Q000187:drug effects; D001616:beta-Galactosidase / Q000235:genetics
D016587:Antimutagenic Agents; D004396:Coloring Agents; D004269:DNA, Bacterial; D001616:beta-Galactosidase; D003474:Curcumin