[The assay of enzyme activity in EIA--colorimetric, fluorometric and luminometric method].
The method of enzyme activity related in enzyme immunoassay was described. The detection methods were colorimetric, fluorometric and chemi- or bioluminometric assay for enzyme, and the sensitivity of three detection methods were compared. The order of detection limit was chemi- or bioluminometry > fluorometry > colorimetry. But it is important that the selection of detection method of enzyme will make fit on your desirable sensitivity of analysis.
D000469:Alkaline Phosphatase / Q000032:analysis; D003124:Colorimetry; D005470:Fluorometry; D006735:Horseradish Peroxidase / Q000032:analysis; D007124:Immunoenzyme Techniques; D008163:Luminescent Measurements; D012680:Sensitivity and Specificity; D001616:beta-Galactosidase / Q000032:analysis
D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase; D001616:beta-Galactosidase