Formation of pivaloylcarnitine in isolated rat heart cells.
Pivaloyl-containing antibiotics and pivalic acid in man or rat have been reported to cause increased urinary carnitine loss secondary to pivaloylcarnitine generation. Pivaloylcarnitine concentration was especially high in heart after administration of pivalic acid or pivampicillin in vivo. Formation of pivaloylcarnitine was therefore studied in isolated rat heart cells in the presence of sodium pivalate. Formation of pivaloylcarnitine in rat heart cells increased with incubation time, after a lag time from 0 to 2 h and linearly up to 6 h. The formation increased with increasing concentration of sodium pivalate, followed Michaelis-Menten kinetics with an apparent Km = 348 +/- 10 microM and Vmax = 116 +/- 20 pmol.mg protein-1.h-1. Bromoacetylcarnitine inhibited the pivaloylcarnitine formation to Ki = 116 +/- 43 microM and Vmax = 107 +/- 14 pmol.mg protein-1.h-1. The uptake of carnitine in heart cells was suppressed 62-74% by deoxycarnitine (40 microM) and D-carnitine (200 microM), and 95% by NaF (5 mM), NaN3 (500 microM) or at temperature 4 degrees C. Pivaloylcarnitine inhibited carnitine uptake to 33-35% of the controls, while sodium pivalate did not. More than 90% of intracellular pivaloylcarnitine was released from the heart cells after 18 h of incubation in the absence of sodium pivalate and L-carnitine. These data show that pivalate is readily converted to pivaloylcarnitine in heart cells, in contrast to the limited conversion in hepatocytes.
D000108:Acetylcarnitine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001622:Betaine / Q000031:analogs & derivatives / Q000494:pharmacology; D002331:Carnitine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology; D002478:Cells, Cultured; D007700:Kinetics; D009206:Myocardium / Q000378:metabolism*; D010421:Pentanoic Acids / Q000378:metabolism; D010917:Pivampicillin / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D010421:Pentanoic Acids; D010917:Pivampicillin; C033813:bromoacetylcarnitine; D001622:Betaine; C002889:gamma-butyrobetaine; D000108:Acetylcarnitine; C005566:pivalic acid; C054040:pivaloylcarnitine; D002331:Carnitine