title: Fibronectin peptide DRVPHSRNSIT and fibronectin receptor peptide DLYYLMDL arrest gastrulation of Rana pipiens.
abstract: Gastrulation is characterized by dramatic cell migration which is thought to require the interaction of cell adhesion molecules with extracellular molecules. We have tested two novel peptides, a fibronectin peptide and a fibronectin receptor peptide, for their effects on gastrulation of the leopard frog Rana pipiens. The fibronectin peptide DRVPHSRNSIT corresponds to residues 1373-1383 of the cell-binding domain of fibronectin; the receptor peptide DLYYLMDL corresponds to residues 124-131 of beta 1 subunit of a variety of integrins including alpha 5 beta 1. Either of these peptides significantly inhibited gastrulation after being microinjected into mid-blastulae. These results indicate that these sequences may correspond to the ligand/receptor interaction sites of fibronectin and its receptor(s).
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005353:Fibronectins / Q000737:chemistry / Q000494:pharmacology*; D005775:Gastrula / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008845:Microinjections; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000008:administration & dosage / Q000494:pharmacology*; D011894:Rana pipiens; D017469:Receptors, Fibronectin / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D005353:Fibronectins; D010446:Peptide Fragments; D017469:Receptors, Fibronectin