Genotyping apolipoprotein B signal peptide insertion/deletion: a comparison of three methods.
Three different ways of detecting insertion/deletion (Ins/Del) genotypes of the apolipoprotein B gene encoding signal peptide were compared. Unequal quantities of the two alleles were observed in 50 Serbian subjects. The low abundance allele was clearly visible only when a modified method of silver staining for polyacrylamide gels was used. This method is more reliable for Ins/Del polymorphism genotyping, avoiding misclassifications by conventional ethidium bromide staining of both agarose and polyacrylamide gels.
D000483:Alleles; D001055:Apolipoproteins B / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D021382:Protein Sorting Signals / Q000235:genetics*; D017384:Sequence Deletion; D016622:Silver Staining
D001055:Apolipoproteins B; D017931:DNA Primers; D021382:Protein Sorting Signals