Predicted structure and possible ionmotive mechanism of the sodium-linked NADH-ubiquinone oxidoreductase of Vibrio alginolyticus.
Two groups have now published sequences of the six genes contained in the operon coding for the sodium-linked NADH-ubiquinone oxidoreductase of Vibrio alginolyticus. Sequence analyses indicate that this enzyme is unrelated to other known respiratory NADH dehydrogenases. A search for cofactor motifs suggests that the enzyme contains only one FAD, a ferredoxin-type iron sulphur centre, and the NADH-binding site. These are all located on NqrF, a subunit that can be recognized as a new member of a large diverse family of NAD(P)H-oxidizing flavoenzymes. A possible model of ion-coupling is presented, based upon this new information.
D000595:Amino Acid Sequence; D042967:Electron Transport Complex I; D005420:Flavoproteins / Q000737:chemistry; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D008961:Models, Structural; D008969:Molecular Sequence Data; D009247:NADH, NADPH Oxidoreductases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016366:Open Reading Frames; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D012964:Sodium / Q000378:metabolism; D014733:Vibrio / Q000201:enzymology*
D005420:Flavoproteins; D046911:Macromolecular Substances; D012964:Sodium; D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I