Impaired survival and growth in immunosuppressed young rats with lethal short gut syndrome and a small bowel transplant: an effect of cyclosporine.
Neonates and growing individuals have increased nutritional demands as compared with adults. To determine the functional ability of an intestinal graft to allow survival and growth, an otherwise lethal short gut model should be used (resection of both the entire small bowel and the cecum). In this study the authors investigated the survival and growth in young rats (80 to 125 g) with this lethal short gut syndrome (SGS) and either syngeneic or allogeneic segmental small bowel transplantation (SBTx). Additionally they sought to determine the effect of therapeutical doses of cyclosporine (CyA) in young, growing rats. To avoid total parenteral nutrition in rats undergoing SBTx, surgery was carried out in two steps: after segmental SBTx of a 25-cm jejunal graft, SGS was created 2 weeks later. Lewis rats underwent 1: Syngeneic segmental SBTx + SGS (n = 7); 2: Allogeneic segmental SBTx (donor: Lewis Brown Norway F1) + SGS + CyA (15 mg/kg/d for 7 days, then every other day for 21 days) (n = 9); 3: Syngeneic segmental SBTx + SGS + CyA as in group 2 (n = 5); 4: SGS alone (n = 5): 5: small bowel resection alone (n = 5); 6: sham laparotomy twice (n = 5); 7: sham laparotomy twice + CyA as in group 2 (n = 6). Weight, general condition, and nutritional serum variables were followed up regularly for 4 months. Rats with resection of small bowel survived but did not grow. Rats with small bowel resection + cecectomy died within 5 days.(ABSTRACT TRUNCATED AT 250 WORDS)
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001835:Body Weight; D002432:Cecum / Q000601:surgery; D003404:Creatinine / Q000097:blood; D016572:Cyclosporine / Q000494:pharmacology*; D006128:Growth / Q000187:drug effects; D016867:Immunocompromised Host; D007421:Intestine, Small / Q000601:surgery; D007583:Jejunum / Q000187:drug effects / Q000637:transplantation*; D007813:Laparotomy; D008297:Male; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D012709:Serum Albumin / Q000032:analysis; D012778:Short Bowel Syndrome / Q000503:physiopathology* / Q000601:surgery*; D015996:Survival Rate; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic; D014280:Triglycerides / Q000097:blood; D014508:Urea / Q000097:blood
D012709:Serum Albumin; D014280:Triglycerides; D016572:Cyclosporine; D014508:Urea; D003404:Creatinine