Preparation of antibodies to catecholamines and metabolites--syntheses of various immunogens and characterization of the resulting antibodies.
The synthesis of antigens of the haptens p-tyramine, octopamine, synephrine, dopamine, normetanephrine, beta-(3,4-dichlorophenyl)-ethylamine and 3-fluoro-tyramine is described. The coupling of the haptens to the carrier protein was performed either via the side chain by means of succinic anhydride, or through the phenolic ring via an aminomethyl group which was introduced by a Mannich reaction. Antisera to these haptens were produced in rabbits by immunization with these antigens. The antisera were characterized by determination of titer and specificity (p-tyramine, synephrine, normetanephrine). The possibility is discussed of using antisera to p-hydroxyphenylethylamine for a radioimmunological determination of catecholamines.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D002352:Carrier Proteins / Q000378:metabolism; D002395:Catecholamines / Q000276:immunology*; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D006241:Haptens / Q000276:immunology; D011817:Rabbits
D000941:Antigens; D002352:Carrier Proteins; D002395:Catecholamines; D006241:Haptens