title: The influence of proteins on silver staining of nucleic acids following polyacrylamide gel electrophoresis.
abstract: The influence, on silver staining, of proteins and restriction enzymes in polymerase chain reaction (PCR) products was studied in 12-20% polyacrylamide gels. For small DNA fragments (74, 41 and 33 bp) best results were achieved using 20% polyacrylamide gels. In 12% gels, restriction enzymes and also proteins used for PCR (bovine serum albumin, Taq-DNA polymerase) interfere with silver staining of nucleic acids.
mesh_terms: D004247:DNA / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D009132:Muscles / Q000166:cytology; D016133:Polymerase Chain Reaction / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D016622:Silver Staining
chemical_list: D011506:Proteins; D004247:DNA