title: Effect of endogenous insulin on pancreatic exocrine secretion in perfused dog pancreas.
abstract: Canine pancreas was perfused with an intraarterial infusion of Krebs-Ringer bicarbonate solution containing 5% dog red blood cells, 0.1% bovine serum albumin, and 3% dextran at 15 ml/min, while portal effluent was continuously collected. Pancreatic juice was obtained in 15-min samples via main pancreatic duct cannulation. After a 1-h basal period, secretin and cholecystokinin-8 (CCK), at doses of 2.5 ng.min-1 each, were simultaneously infused for 10 min, with background infusion of a normal rabbit serum (NRS) or an antiinsulin serum (Anti-I) in 5 ml each via a sidearm of the intraarterial catheter. The infusion of secretin and CCK resulted in a significant increase in pancreatic bicarbonate and protein secretion during the infusion of NRS, whereas the pancreatic secretory response of bicarbonate and protein was profoundly suppressed by the infusion of Anti-I in six pancreata so studied. This suppression by Anti-I coincided with significant increases in somatostatin and pancreatic polypeptide levels in portal venous effluent. In three additional pancreata, simultaneous infusions of Anti-I with antisomatostatin (5 ml) and antipancreatic polypeptide (5 ml) serum failed to inhibit the pancreatic exocrine secretion. These results indicate that secretin- and CCK-stimulated pancreatic secretion of bicarbonate and protein depends heavily on local action of insulin. The suppression by Anti-I of pancreatic secretion is mediated, in part, by local releases of somatostatin and pancreatic polypeptide. Thus, the insuloacinar axis plays an important regulatory role in pancreatic exocrine secretion in the dog, and it involves at least three islet hormones including insulin, somatostatin, and pancreatic polypeptide.
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D002766:Cholecystokinin / Q000494:pharmacology; D004285:Dogs; D007116:Immunization, Passive; D007328:Insulin / Q000276:immunology / Q000502:physiology*; D010179:Pancreas / Q000378:metabolism*; D010191:Pancreatic Polypeptide / Q000276:immunology / Q000378:metabolism; D010477:Perfusion; D011506:Proteins / Q000378:metabolism; D012633:Secretin / Q000494:pharmacology; D013004:Somatostatin / Q000276:immunology / Q000378:metabolism
chemical_list: D001639:Bicarbonates; D007328:Insulin; D011506:Proteins; D012633:Secretin; D013004:Somatostatin; D010191:Pancreatic Polypeptide; D002766:Cholecystokinin