Processing and activation of recombinant mouse mastocytoma histidine decarboxylase in the particulate fraction of Sf9 cells by porcine pancreatic elastase.
Mature 53 kDa histidine decarboxylase (HDC) peptide is produced from a precursor 74 kDa peptide. The mechanism of specific cleavage by processing enzyme is unknown. Using the recombinant mouse 74 kDa HDC, we found that porcine pancreatic elastase specifically converted the inactive 74 kDa HDC to its active form of 53 kDa HDC.
D000818:Animals; D002460:Cell Line; D002847:Chromatography, Agarose; D010450:Endopeptidases / Q000378:metabolism; D006640:Histidine Decarboxylase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D015151:Immunoblotting; D012515:Mast-Cell Sarcoma / Q000201:enzymology; D051379:Mice; D008970:Molecular Weight; D010179:Pancreas / Q000201:enzymology; D010196:Pancreatic Elastase / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012995:Solubility; D013552:Swine
D011993:Recombinant Fusion Proteins; D010450:Endopeptidases; D010196:Pancreatic Elastase; D006640:Histidine Decarboxylase