title: Effects of cold exposure and hibernation on renal Na,K-ATPase of the jerboa Jaculus orientalis.
abstract: Changes in activity and abundance of renal Na,K-ATPase were evaluated during cold exposure and hibernation of the jerboa Jaculus orientalis by measuring the hydrolytic activity, the number of units and the transport activity of Na,K-ATPase in isolated nephron segments. As compared to controls, jerboas exposed to cold (6 degrees C) for 4-5 weeks displayed mild diuresis, decreased urinary osmolality and increased kaliuresis. In cold-exposed jerboas, Na,K-ATPase hydrolytic activity was reduced in the medullary thick ascending limb and enhanced in the cortical and outer medullary collecting duct, whereas it was not altered in other nephron segments. The number of Na,K-ATPase units and the activity of Na,K-pump, determined by [3H]-ouabain binding and by ouabain-sensitive rubidium uptake respectively, changed in parallel with the hydrolytic activity in the medullary thick ascending limb and cortical collecting duct. The maximal rate of activity (Vmax) of Na,K-ATPase was not modified further during hibernation. Thus, cold exposure, but not the onset of hibernation, induces segment-specific changes in the abundance and activity of Na,K-ATPase units which are likely to be related to the entry into hibernation, but not to the maintenance of some renal functions during deep hibernation.
mesh_terms: D000818:Animals; D003080:Cold Temperature; D004231:Diuresis / Q000187:drug effects; D005260:Female; D005508:Food Deprivation; D006605:Hibernation / Q000502:physiology*; D007668:Kidney / Q000201:enzymology* / Q000502:physiology; D007684:Kidney Tubules / Q000201:enzymology; D007685:Kidney Tubules, Collecting / Q000201:enzymology / Q000378:metabolism; D007700:Kinetics; D008297:Male; D010042:Ouabain / Q000378:metabolism; D012377:Rodentia / Q000378:metabolism*; D012413:Rubidium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D014563:Urodynamics / Q000187:drug effects
chemical_list: D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium