Location in the source chromosome of the 180-kb minichromosome of Leishmania major and characterization of the novel junction.
The 180-kb LD1 minichromosome of Leishmania major (M180) is a large inverted duplication which arises spontaneously from a megabasic chromosome. In this work this locus has been located in the source chromosome at a telomeric position. Both the novel junction created in the middle of the minichromosome, as well as its counterpart region in the source chromosome were cloned and sequenced. Two inverted repeats, which could give rise to two imperfect stem-loops, and an A + T-rich DNA sequence were the only particular features observed in this region. A putative model for the formation of the minichromosome is proposed.
D000818:Animals; D001483:Base Sequence; D002875:Chromosomes / Q000648:ultrastructure*; D003001:Cloning, Molecular; D003434:Crossing Over, Genetic; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D005784:Gene Amplification; D018320:Leishmania major / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D016615:Telomere / Q000648:ultrastructure
D017931:DNA Primers; D016054:DNA, Protozoan