title: Magnetic fields applied to collagen-coated ferric oxide beads induce stretch-activated Ca2+ flux in fibroblasts.
abstract: The ability to apply controlled forces to the cell membrane may enable elucidation of the mechanisms and pathways involved in signal transduction in response to applied physical stimuli. We have developed a magnetic particle-electromagnet model that allows the application of controlled forces to the plasma membrane of substrate-attached fibroblasts. The system allows applied forces to be controlled by the magnitude of the magnetic field and by the surface area of cell membrane covered with collagen-coated ferric beads. Analysis by single-cell ratio fluorimetry of fura 2-loaded cells demonstrated large calcium transients (50-300 nM) in response to the magnetic force applications. Experiments using either the stretch-activated channel blocker gadolinium chloride or ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid to eliminate external calcium ions, or addition of extracellular manganese ions, indicated that there was a calcium influx through putative stretch-activated channels. The probability of a calcium influx in single cells was increased by higher surface bead loading and the degree of cell spreading. Depolymerization of actin filaments by cytochalasin D increased the amplitude of calcium response twofold. The regulation of calcium flux by filamentous actin content and by cell spreading indicates a possible modulatory role for the cytoskeleton in channel sensitivity. Magnetic force application to beads on single cells provides a controlled model to study mechanisms and heterogeneity in physical force stimulation of cation-permeable channels.
mesh_terms: D000199:Actins / Q000502:physiology; D002021:Buffers; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D002478:Cells, Cultured; D003094:Collagen; D004594:Electrophysiology; D005110:Extracellular Space / Q000378:metabolism; D005290:Ferric Compounds; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D008280:Magnetics; D008863:Microspheres; D010812:Physical Stimulation; D011188:Potassium / Q000494:pharmacology
chemical_list: D000199:Actins; D002021:Buffers; D015220:Calcium Channels; D005290:Ferric Compounds; C000499:ferric oxide; D003094:Collagen; D011188:Potassium; D002118:Calcium