Detection of measles virus RNA in urine specimens from vaccine recipients.
Analysis of urine specimens by using reverse transcriptase-PCR was evaluated as a rapid assay to identify individuals infected with measles virus. For the study, daily urine samples were obtained from either 15-month-old children or young adults following measles immunization. Overall, measles virus RNA was detected in 10 of 12 children during the 2-week sampling period. In some cases, measles virus RNA was detected as early as 1 day or as late as 14 days after vaccination. Measles virus RNA was also detected in the urine samples from all four of the young adults between 1 and 13 days after vaccination. This assay will enable continued studies of the shedding and transmission of measles virus and, it is hoped, will provide a rapid means to identify measles infection, especially in mild or asymptomatic cases.
D000328:Adult; D001483:Base Sequence; D006801:Humans; D007223:Infant; D008457:Measles / Q000517:prevention & control / Q000652:urine / Q000821:virology*; D008459:Measles virus / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000652:urine*; D014611:Vaccination
D012367:RNA, Viral