Lipid peroxidation as an indicator of biocompatibility in haemodialysis.
Peroxidation of cell membrane (phosphatidylcholine hydroperoxide, PCOOH) was quantitatively assessed using a chemiluminescence-HPLC system to clarify whether haemodialysis patients are damaged by oxidative stress. Patients were divided into two groups, one dialysed with conventional cellulose membranes, and the other with cellulose triacetate for 3 months, subsequently followed by a crossover study of each membrane for 3 more months. The mean value of PCOOH in haemodialysis patients was 508.5 +/- 208.7 pmol/ml (P < 0.01 vs normal controls), which showed a statistically insignificant tendency to increase during each haemodialysis session of 4 h. Two years after commencing haemodialysis, PCOOH was maintained within almost the same range, but significantly greater than normal. There was no significant tendency between an index of aortic sclerosis and plasma PCOOH. However, three patients using conventional cellulose membranes died of myocardial infarction. Plasma PCOOH increased after the commencement of haemodialysis. This tendency was more notable in patients using conventional cellulose membrane compared to the cellulose triacetate. Cellulose triacetate, which we thought to be more biocompatible, did not necessarily produce lipid peroxide. We conclude that it is very important to check production of oxygen radicals when developing new membranes.
D001672:Biocompatible Materials / Q000378:metabolism*; D018592:Cross-Over Studies; D005260:Female; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D010713:Phosphatidylcholines / Q000097:blood / Q000378:metabolism*; D006435:Renal Dialysis / Q000295:instrumentation*; D014511:Uremia / Q000276:immunology / Q000378:metabolism / Q000628:therapy*
D001672:Biocompatible Materials; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; C057590:phosphatidylcholine hydroperoxide