Translocation of insulin-regulated glucose transporter is stimulated by long-chain 1,2-diacylglycerol in rat adipocytes.
The long-chain diacylglycerol 1,2-dimyristoylglycerol emulsified with taurodeoxycholate has been shown to potently stimulate glucose transport in isolated rat adipocytes (Stråfors, Nature 335, 554-556 (1988)). We now report that this 1,2-diacylglycerol in the presence of taurodeoxycholate, similarly to insulin, induced translocation of the insulin-regulated glucose transporter (GLUT-4) from a microsomal membrane compartment to the plasma membrane. H4IIE hepatoma cells expressed mRNA for GLUT-1, but not for GLUT-4. In these, otherwise insulin-responsive, cells diacylglycerol or insulin had only a marginal effect on glucose transport.
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D004075:Diglycerides / Q000494:pharmacology*; D005079:Excipients; D005947:Glucose / Q000378:metabolism; D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D007328:Insulin / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013657:Taurodeoxycholic Acid; D014407:Tumor Cells, Cultured
C011439:1,2-diacylglycerol; D004075:Diglycerides; D005079:Excipients; D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; C495679:Slc2a1 protein, rat; C495694:Slc2a4 protein, rat; D013657:Taurodeoxycholic Acid; D005947:Glucose