The carboxyl extensions of two rat ubiquitin fusion proteins are ribosomal proteins S27a and L40.
Two rat recombinant cDNAs were characterized; they encode fusion proteins that have at their NH2 terminus the conserved 76 amino acid ubiquitin and at their carboxyl terminus the extension ribosomal proteins S27a (80 amino acids and a molecular weight of 9,397) or L40 (52 amino acids and a molecular weight of 6,177). The fusion proteins are processed in a reticulocyte lysate to ubiquitin and either S27a or L40. Hybridization of cDNAs to digests of nuclear DNA suggests that there are 14 to 19 copies of the S27a, and 6 to 10 of the L40, genes. The mRNA for ubiquitin-S27a has about 700 nucleotides and ubiquitin-L40 about 650. Ribosomal proteins S27a and L40 contain zinc finger motifs of the C2-C2 variety. S27a and L40 are related to ribosomal proteins from other species.
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry*; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D014452:Ubiquitins / Q000096:biosynthesis / Q000737:chemistry*; D016335:Zinc Fingers
D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D012269:Ribosomal Proteins; D014452:Ubiquitins; C096228:ribosomal protein L40, rat; C096227:ribosomal protein S27a; D004247:DNA