title: [Cloning fragments of the RNA polymerase gene of an attenuated variant of the foot-and-mouth disease virus A22].
abstract: The cDNA fragments complementary to RNA-polymerase gene and 3'-untranslated genome region of attenuated foot-and-mouth disease virus strain A(22)645 have been synthesized and cloned into a plasmid vector pUC19 in E. coli JM109. The cloned cDNA fragments were characterized as to their size, orientation towards the plasmid, and localization in the virus genome. Restriction maps for complete gene and two cDNA clones were constructed.
mesh_terms: D005537:Aphthovirus / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D010446:Peptide Fragments / Q000235:genetics*; D010957:Plasmids; D014613:Vaccines, Attenuated / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D010446:Peptide Fragments; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D012321:DNA-Directed RNA Polymerases
vernacular_title: Klonirovanie fragmentov gena RNK-polimerazy attenuirovannogo varianta virusa iashchura A22.