A synthetic sialic acid analog that is resistant to the receptor-destroying enzyme can be used by influenza C virus as a receptor determinant for infection of cells.
A synthetic sialic acid analog, N-acetyl-9-acetamido-9-deoxy-neuraminic acid, can be used by influenza C virus as a receptor determinant for attachment to cells. In contrast to the natural determinant, N-acetyl-9-O-acetylneuraminic acid, the synthetic sialic acid is resistant to the action of the receptor-destroying acetylesterase of this virus. The sialic acid analog was enzymatically transferred to the surface of Madin-Darby canine kidney cells that are resistant to infection because of a lack of receptors. Influenza C virus was able to infect the modified cells through requiring a 10-fold larger amount of the sialic acid analogue on the cell surface compared to the natural receptor determinant. The quantitative difference is accounted for mainly by a less efficient binding of influenza C virus to the analog. Thus, in our system, inactivation of the receptor by the viral esterase is not required for the initiation of an influenza C virus infection.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000821:virology; D003568:Cytidine Monophosphate / Q000494:pharmacology; D004285:Dogs; D004950:Esterases / Q000378:metabolism*; D009982:Gammainfluenzavirus / Q000378:metabolism*; D007668:Kidney; D015394:Molecular Structure; D011991:Receptors, Virus / Q000737:chemistry* / Q000378:metabolism; D012794:Sialic Acids / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship
C096863:N-acetyl-9-acetamido-9-deoxyneuraminic acid; D011991:Receptors, Virus; D012794:Sialic Acids; D004950:Esterases; D003568:Cytidine Monophosphate