Lineage switch of a mouse pre-B cell line (SPGM-1) to macrophage-like cells after incubation with phorbol ester and calcium ionophore.
The mouse CD5 positive pre-B cell line SPGM-1 can be induced to switch its lineage commitment towards macrophage differentiation by treatment with a combination of phorbol ester and a calcium ionophore. When cultured with these reagents the pre-B cells ceased to proliferate and rapidly became adherent to plastic surfaces. This morphological change was accompanied by the loss of pre-B cell-specific surface markers, such as PB76 and most prominently the mu heavy chain of the immunoglobulin receptor complex. In addition, the mRNA of the surrogate light chain lambda 5 disappeared while the induction of lysozyme mRNA could be detected. Differentiated SPGM-1 cells phagocytosed latex beads and showed nonspecific esterase activity. The high efficiency and speed of differentiation in this cellular system makes SPGM-1 a highly suitable model for studying the phenomenon of lineage switching during hemopoesis.
D000818:Animals; D000906:Antibodies; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes; D015152:Blotting, Northern; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005434:Flow Cytometry; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression / Q000187:drug effects; D016236:Genes, fms; D007377:Interleukin-3 / Q000494:pharmacology*; D015759:Ionomycin / Q000494:pharmacology*; D007476:Ionophores / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D051379:Mice; D009113:Muramidase / Q000096:biosynthesis; D010587:Phagocytosis / Q000187:drug effects / Q000502:physiology; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000906:Antibodies; D000954:Antigens, Surface; D007377:Interleukin-3; D007476:Ionophores; D012333:RNA, Messenger; D015759:Ionomycin; D016186:Receptor, Macrophage Colony-Stimulating Factor; D009113:Muramidase; D013755:Tetradecanoylphorbol Acetate