Increase of protooncogene c-myc mRNA levels by low density lipoprotein in vascular smooth muscle cells.
The effect of low density lipoprotein (LDL) on the intracellular mRNA concentration of the protooncogene c-myc was studied in freshly isolated bovine vascular smooth muscle cells and in the rat aortic smooth muscle cell line A7r5. Northern analysis showed that LDL increased the mRNA levels of c-myc in both cell lines, the stimulation being 2-fold after 2 h incubation at a concentration of 50 micrograms LDL-protein/ml. High density lipoprotein (HDL) had no effect on c-myc mRNA levels in A7r5 cells. These results demonstrate that LDL, but not HDL, increases intracellular concentrations of c-myc in two different aortic smooth muscle cell lines.
D000818:Animals; D001011:Aorta / Q000187:drug effects; D002417:Cattle; D002460:Cell Line; D016259:Genes, myc; D006801:Humans; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D012333:RNA, Messenger / Q000187:drug effects*; D051381:Rats
D008077:Lipoproteins, LDL; D012333:RNA, Messenger