Inhibition by cholesterylphosphorylserine of T-cell-mediated immune responses in mice.
The synthetic analogue of phosphatidylserine, cholesterylphosphorylserine (CPHS) inhibits T-cell-mediated immune responses in mice. Tested in cultured mouse spleen cells, CPHS inhibits concanavalin A-induced activation of DNA synthesis (IC50, 3.5 microM). Injected i.p. during the efferent phase, CPHS (25-100 mg/kg) inhibits the manifestations of delayed-type of hypersensitivity. The compound (25 mg/kg i.p., daily) reduces the acute graft-versus-host reaction when given for 5 days to donor mice before the isolation of spleen cells used for the inoculum. These data suggest that the addition of a phosphorylserine group to a steroid ring may produce immunoregulatory compounds.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000276:immunology / Q000494:pharmacology*; D006087:Graft vs Host Reaction / Q000187:drug effects; D006968:Hypersensitivity, Delayed / Q000517:prevention & control; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008407:Mast Cells / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010718:Phosphatidylserines / Q000276:immunology / Q000494:pharmacology*; D010768:Phosphoserine / Q000031:analogs & derivatives* / Q000276:immunology / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000187:drug effects*
D000276:Adjuvants, Immunologic; D010718:Phosphatidylserines; C097061:cholesterylphosphorylserine; D010768:Phosphoserine; D002784:Cholesterol