Occurrence, in Crohn's disease, of antibodies directed against a species-specific recombinant polypeptide of Mycobacterium paratuberculosis.
Sera from patients with Crohn's disease and control were analyzed by an enzyme-linked immunosorbent assay based on the Mycobacterium paratuberculosis-specific recombinant polypeptide a362. Anti-a362 immunoglobulin G (IgG) (P < 0.05) and IgA (P < 0.001) titers were higher in patients with Crohn's disease than in controls. A monomodal Gaussian distribution of anti-a362 IgA levels were found for controls, and a bimodal distribution was found for patients with Crohn's disease. An M. paratuberculosis etiology is suggested for the 36% of patients with Crohn's disease who had an anti-a362 IgA level higher than that of controls.
D000907:Antibodies, Bacterial / Q000737:chemistry*; D000942:Antigens, Bacterial / Q000276:immunology*; D003424:Crohn Disease / Q000276:immunology* / Q000382:microbiology; D006801:Humans; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology*; D010455:Peptides / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D010455:Peptides; D011994:Recombinant Proteins