Compound D609 inhibits phorbol ester-stimulated phospholipase D activity and phospholipase C-mediated phosphatidylethanolamine hydrolysis.
Tricyclodecan-9-yl-xanthogenate (compound D609) has recently been used in various cellular systems to specifically inhibit the activity of phosphatidylcholine (PtdCho)-directed phospholipase C (PLC). Here we show that in intact NIH 3T3 fibroblasts, concentrations of D609 (35 to 50 micrograms/ml) which have been used to inhibit PLC activity also significantly inhibit phorbol ester-induced phospholipase D-mediated hydrolysis of both PtdCho and phosphatidylethanolamine (PtdEtn). In addition, in isolated membranes compound D609 also inhibited PLC-mediated PtdEtn hydrolysis. The results indicate that compound D609 cannot be considered as a specific inhibitor of PtdCho-directed PLC activity.
D016475:3T3 Cells; D000818:Animals; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D002462:Cell Membrane / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006868:Hydrolysis; D007700:Kinetics; D008563:Membrane Lipids / Q000378:metabolism; D051379:Mice; D009636:Norbornanes; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D010739:Phospholipase D / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013859:Thiocarbamates; D013871:Thiones / Q000494:pharmacology*; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D001952:Bridged-Ring Compounds; D008563:Membrane Lipids; D009636:Norbornanes; D010714:Phosphatidylethanolamines; D010726:Phosphodiesterase Inhibitors; D013859:Thiocarbamates; D013871:Thiones; C046498:tricyclodecane-9-yl-xanthogenate; D010738:Type C Phospholipases; D010739:Phospholipase D; D002794:Choline