Isozyme analysis of human normal polymorphonuclear leukocyte phosphofructokinase.
Phosphofructokinase (PFK) from human polymorphonuclear leukocytes (PMN) was characterized by immunological titration with subunit specific antibodies, column chromatography on QAE-Sephadex and SDS-polyacrylamide gel electrophoresis. Two different isozymes, M-type and L-type, were found. The M(r) values of the M and L subunits were 79,500 +/- 1,914 and 74,250 +/- 1,258, respectively. The two isozymes presented different kinetic and regulatory properties. The results suggest that PFK from human normal PMN is a mixture of M-type and L-type homotetramers, mainly, with possible minor heterotetrameric forms.
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005635:Fructosediphosphates / Q000494:pharmacology; D006801:Humans; D007163:Immunosorbent Techniques; D007527:Isoenzymes / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D007700:Kinetics; D046911:Macromolecular Substances; D009504:Neutrophils / Q000201:enzymology*; D010732:Phosphofructokinase-1 / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism
D002951:Citrates; D004791:Enzyme Inhibitors; D005635:Fructosediphosphates; D007527:Isoenzymes; D046911:Macromolecular Substances; D019343:Citric Acid; D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1; C029063:fructose-1,6-diphosphate