Enhancement of NGF gene expression in rat brain by the memory-enhancing peptide AVP(4-8).
Northern blot analysis of nerve growth factor (NGF) was used to evaluate the effect of exogenous AVP(4-8) on the transcription of NGF gene in rat brain. NGF expression was found to be significantly enhanced by exogenous AVP(4-8) in the hippocampus as well as in the cerebral cortex in a time period of 12 h. This effect was inhibited by an antagonist to AVP(4-8). In addition, gel mobility shift assay was also used to observe the in vitro expression of c-fos gene in rat hippocampal slices. Our results suggest that NGF gene is one of the target genes responsible for memory-enhancing responses induced by AVP(4-8) and that the enhancement of NGF gene expression may share the signaling pathway mediated by AVP(4-8) receptor and c-fos gene expression.
D000818:Animals; D001127:Arginine Vasopressin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D001483:Base Sequence; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000187:drug effects*; D016762:Genes, fos; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008568:Memory / Q000187:drug effects*; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics*; D015345:Oligonucleotide Probes; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
C094362:(phenylmethoxy)carbonyl-asparaginyl-(cysteinyl)cysteinyl-prolyl-arginine; D009414:Nerve Growth Factors; D015345:Oligonucleotide Probes; D010446:Peptide Fragments; D012333:RNA, Messenger; D001127:Arginine Vasopressin; C072508:argipressin (4-8)