title: RNA polymerase II transcribes Dictyostelium untranslatable gene, dutA, specifically in the developmental phase.
abstract: In Dictyostelium discoideum, a novel type of RNA (dutA RNA), which is untranslatable, cytosolic and 1.3 kb in size, appears specifically after the aggregation stage [Yoshida, H. et al. (1994) Nucleic Acids Res. 22, 41-46]. We here show that the dutA gene is transcribed by RNA polymerase II, based on its alpha-amanitin sensitive nature of in vitro transcriptional activity. We also show that the stage-specific accumulation of dutA RNA is primarily due to the stage-specific enhancement of the transcriptional activity of the gene.
mesh_terms: D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D015152:Blotting, Northern; D004023:Dictyostelium / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007700:Kinetics; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012331:RNA, Fungal / Q000032:analysis / Q000096:biosynthesis*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000546:Amanitins; D012331:RNA, Fungal; D012319:RNA Polymerase II