title: Influence of sE-selectin and L-selectin on the regulation of cell migration during chronic periodontitis.
abstract: Soluble endothelial leukocyte adhesion molecule-1 (sE-selectin) levels in peripheral blood (PB) and gingival capillary blood (GCB) of both healthy donors (HD) and patients with adult periodontitis (AP) were assayed by ELISA. Binding of sE-selectin to polymorphonuclear neutrophils (PMN) from PB, GCB and crevicular fluid (GF), and expression of L-selectin and sialyl-Lewisx (sLex) on these cells were analyzed by immunofluorescence and flow cytometry. No significantly enhanced serum levels of sE-selectin in patients with AP, compared to HD (28 +/- 5 ng/ml vs 19 +/- 3 ng/ml, respectively), and no differences in the concentration of sE-selectin in GCB (16 +/- 1 ng/ml vs 16 +/- 2 ng/ml, respectively) were observed. On PB-PMN no significant differences in the expression of L-selectin and sLex were found and binding of sE-selectin to PB-PMN was comparable between HD and patients with AP. Binding of sE-selectin to GCB-PMN was significantly higher in patients with AP compared to HD (mean channel fluorescence (MCF) = 88.5 +/- 13.2 vs MCF = 24.2 +/- 5.3, respectively). The expression of sLex on GCB-PMN did not differ significantly between the two groups. A significant decrease in the expression of the adhesion molecule L-selectin on GCB-PMNs compared to PB-PMN was found in patients with AP but not in HD. CF-PMN showed decreased expression of both L-selectin and sLex compared to PMN from PB and GCB, both in HD and patients with AP. Taken together, these data suggest that PMN from patients with AP had reduced selectin-mediated adhesive capabilities to inflamed gingival endothelium.
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002448:Cell Adhesion / Q000276:immunology*; D002464:Cell Migration Inhibition; D002908:Chronic Disease; D019040:E-Selectin / Q000097:blood / Q000276:immunology* / Q000378:metabolism; D004727:Endothelium / Q000166:cytology / Q000276:immunology; D005434:Flow Cytometry; D019085:Fluorescent Antibody Technique, Direct; D005881:Gingiva / Q000098:blood supply / Q000166:cytology / Q000276:immunology; D005883:Gingival Crevicular Fluid / Q000276:immunology; D006801:Humans; D019041:L-Selectin / Q000276:immunology* / Q000378:metabolism; D008024:Ligands; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D009844:Oligosaccharides / Q000378:metabolism; D010518:Periodontitis / Q000097:blood / Q000276:immunology*; D011485:Protein Binding; D000080506:Sialyl Lewis X Antigen
chemical_list: D019040:E-Selectin; D008024:Ligands; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D019041:L-Selectin