Identification of haptoglobin as a natural inhibitor of trypanocidal activity in human serum.
Trypanosomes are protozoan parasites of medical and veterinary importance. Trypanosoma brucei rhodesiense and Trypanosoma brucei gambiense infect humans, causing African sleeping sickness. However, Trypanosoma brucei brucei can only infect animals, causing the disease Nagana in cattle. Man is protected from this subspecies of trypanosomes by a toxic subtype of high density lipoproteins (HDLs) called the trypanosome lytic factor (TLF). The toxic molecule in TLF is believed to be the haptoglobin-related protein that when bound to hemoglobin kills the trypanosome via oxidative damage initiated by its peroxidase activity. The amount of lytic activity in serum varies widely between different individuals with up to a 60-fold difference in activity. In addition, an increase in the total amount of lytic activity occurs during the purification of TLF, suggesting that an inhibitor of TLF (ITLF) exists in human serum. We now show that the individual variation in trypanosome lytic activity in serum correlates to variations in the amount of ITLF. Immunoblots of ITLF probed with antiserum against haptoglobin recognize a 120-kDa protein, indicating that haptoglobin is present in partially purified ITLF. Haptoglobin involvement is further shown in that it inhibits TLF in a manner similar to ITLF. Using an anti-haptoglobin column to remove haptoglobin from ITLF, we show that the loss of haptoglobin coincides with the loss of inhibitor activity. Addition of purified haptoglobin restores inhibitor activity. This indicates that haptoglobin is the molecule responsible for inhibition and therefore causing the individual variation in serum lytic activity.
D000743:Anemia, Hemolytic / Q000097:blood; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D006242:Haptoglobins / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D015151:Immunoblotting; D007700:Kinetics; D008075:Lipoproteins, HDL / Q000037:antagonists & inhibitors / Q000097:blood* / Q000145:classification / Q000302:isolation & purification / Q000494:pharmacology; D014344:Trypanocidal Agents / Q000037:antagonists & inhibitors / Q000097:blood* / Q000302:isolation & purification; D014346:Trypanosoma brucei brucei / Q000187:drug effects*
D006242:Haptoglobins; D008075:Lipoproteins, HDL; D014344:Trypanocidal Agents