Direct effect of 12(S)-hydroxyeicosatetraenoic acid on rat crystalline lens is perturbation of lens normalcy.
The direct effect of 12(S)-hydroxyeicosatetraenoic acid [12(S)-HETE] on rat crystalline lens was investigated in this study. 12(S)-HETE lowered the glutathione (GSH) level and GSH reductase activity in the lens, while accelerating aggregation and insolubilization of lens proteins and production of thiobarbituric acid-reactive substances. The study also indicated that 12(S)-HETE insolubilized alpha-crystalline and induced opacification of the lens when the lens was incubated with 12(S)-HETE. From the results, we presumed that 12(S)-HETE may be oxidized or peroxidized easily and automatically in the air. The substances derived from 12(S)-HETE by oxidation or peroxidation may give the action disordering lens normalcy and induced cataract formation. Thus, the direct effect of 12(S)-HETE may of no benefit to the crystalline lens.
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D000818:Animals; D015153:Blotting, Western; D003459:Crystallins / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005978:Glutathione / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006893:Hydroxyeicosatetraenoic Acids / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008054:Lipid Peroxides / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D003459:Crystallins; D006893:Hydroxyeicosatetraenoic Acids; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D008084:Lipoxygenase; D005980:Glutathione Reductase; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione