Distinct mechanisms of upregulation of type 1A angiotensin II receptor gene expression in kidney and adrenal gland.
We previously demonstrated that type 1A angiotensin II (Ang II) receptor (AT1A) is the predominant renal subtype and is upregulated by a low sodium diet. We have now tested the hypothesis that upregulation of AT1A mRNA induced by sodium deficiency is renal specific and is mediated by activation of type 1 Ang II receptor (AT1). Male Wistar rats were divided into four groups (n = 5 each) and treated for 2 weeks with normal sodium diet (0.5%), normal sodium plus 3 mg/kg per day losartan, low sodium diet (0.07%), or low sodium diet plus losartan. At the end of the 2 weeks, body weight and mean arterial pressure were not different among the four groups (P > .05). Plasma renin activity was elevated by losartan treatment, sodium restriction, or the combination of the two versus control (P < .05). Northern blot analysis showed that the ratio of renal AT1A to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA was increased by losartan treatment, sodium restriction, or the combination of the two versus control (P < .05). In contrast, the ratio of adrenal AT1A to GAPDH mRNA was increased only by sodium restriction versus three other groups (P < .05). Thus, sodium deficiency increases AT1A mRNA in both kidney and adrenal gland, while Ang II receptor blockade by losartan prevents low sodium-induced AT1A mRNA only in adrenal gland.(ABSTRACT TRUNCATED AT 250 WORDS)
D000311:Adrenal Glands / Q000378:metabolism*; D000704:Analysis of Variance; D000804:Angiotensin II / Q000235:genetics*; D000818:Animals; D001794:Blood Pressure; D015152:Blotting, Northern; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics; D007668:Kidney / Q000378:metabolism*; D008297:Male; D012313:RNA / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D011945:Receptors, Angiotensin / Q000235:genetics*; D012083:Renin / Q000097:blood; D015854:Up-Regulation
D015342:DNA Probes; D018076:DNA, Complementary; D012333:RNA, Messenger; D011945:Receptors, Angiotensin; D000804:Angiotensin II; D012313:RNA; D012083:Renin