Guinea pig antibody to C3 component of the sheep complement produced by immunization with a heat-inactivated sheep serum--alcian blue complex.
Normal sheep serum or serum heated at 56 degrees C for 30 min were added to alcian blue solutions. The optical density of alcian blue-heated sheep serum complexes formed were 2-4 times greater than those obtained with unheated serum. Most guinea pigs immunized with alcian blue-heated sheep serum complexes produced antibodies that precipitated the third component (C3) of sheep complement (C') from electrophoresed sheep serum. These antisera also hemagglutinated sheep erythrocyte--rabbit antibody complexes treated with sheep serum (C'). Guinea pigs immunized with alcian blue-normal sheep serum complexes did not react with electrophoresed sheep serum nor with sheep C' in the hemagglutination test.
D000423:Alcian Blue / Q000378:metabolism; D000818:Animals; D000917:Antibody Formation; D003176:Complement C3 / Q000276:immunology* / Q000378:metabolism; D006168:Guinea Pigs; D006358:Hot Temperature; D007106:Immune Sera / Q000276:immunology*; D007114:Immunization; D046911:Macromolecular Substances; D011233:Precipitin Tests; D011489:Protein Denaturation; D012756:Sheep / Q000276:immunology
D003176:Complement C3; D007106:Immune Sera; D046911:Macromolecular Substances; D000423:Alcian Blue