[Application of enzyme-linked immunosorbent assay (ELISA) to the medico-legal identification].
Medico-legal identification of saliva stains and bloodstains was performed by an enzyme-linked immunosorbent assay (ELISA) using a horseradish peroxidase conjugate in combination with the use of monoclonal antibodies. Activity of alpha-amylase in the stains was measured for an identification of saliva using an anti-human amylase antibody, and secretory IgA was detected for a species identification using an anti-IgA antibody. ABO and Lewis blood group antigens were detected using anti-A, anti-B, anti-H, anti-Lea and anti-Leb antibodies. After the solubilization of ABH antigens in blood stains with octyl-beta-D-glucose solution, ABO typing has been performed using fully automated system composed of a unit for blood group reaction based on ELISA and a unit for reading of result based on photometric analysis.
D000017:ABO Blood-Group System; D000818:Animals; D001801:Blood Stains; D004797:Enzyme-Linked Immunosorbent Assay; D005554:Forensic Medicine; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D000516:alpha-Amylases / Q000032:analysis
D000017:ABO Blood-Group System; D007071:Immunoglobulin A, Secretory; D000516:alpha-Amylases