Endothelin-1 expression by human hepatocellular carcinoma.
Plasma levels of endothelin-1 (ET-1) are markedly higher in patients with hepatocellular carcinoma (HCC) than in normal controls. In order to further investigate this, we evaluated ET-1 immunoreactivity and mRNA expression in human HCC tissue. 70% (14/20) of the tumor tissues immunostained positively with ET-1 antibody and a significant association was observed between immunostaining in cells lining the tumor feeding vessels and tumor vascularity as determined by hepatic angiography. Moreover, the neoplastic hepatocytes in the tumors also stained positively with ET-1 antibody. All of the HCC tissue samples which immunostained for ET-1 also expressed ET-1 mRNA, indicating that ET-1 is actively synthesized by the tumors.
D000591:Amino Acid Isomerases / Q000096:biosynthesis; D000792:Angiography; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000098:blood supply / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000096:biosynthesis; D017931:DNA Primers; D016232:Endothelins / Q000032:analysis / Q000096:biosynthesis* / Q000097:blood; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology / Q000000981:diagnostic imaging / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000378:metabolism; D008113:Liver Neoplasms / Q000098:blood supply / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000473:pathology; D008969:Molecular Sequence Data; D019696:Peptidylprolyl Isomerase; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D012016:Reference Values; D014057:Tomography, X-Ray Computed
D002352:Carrier Proteins; D017931:DNA Primers; D016232:Endothelins; D012333:RNA, Messenger; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase