[Cloning and expression of the Escherichia coli thioredoxin gene].
The gene encoding thioredoxin (trxA) was isolated from chromosomal DNA of E. coli HB101 strain using the polymerase chain reaction. The cloned structural gene with a synthetic Shine-Dalgarno sequence was placed under the control of either inducible tac-promoter or a tandem of two strong constitutive promoters A2 and A3 from early region of bacteriophage T7. Both constructions were shown to provide high levels of biosynthesis of recombinant thioredoxin.
D017123:Bacteriophage T7 / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D010441:Peptide Chain Elongation, Translational; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D013879:Thioredoxins / Q000235:genetics*
D017931:DNA Primers; D013879:Thioredoxins
Klonirovanie i ékspressiia gena tioredoksina Escherichia coli.