Comparisons of liver transfer RNA methyltransferase and adenosylmethionine decarboxylase activities of male and female rats.
The activities of liver transfer RNA (tRNA) methyltransferases from control or ovariectomized female rats were found to be higher than those of control or castrated males. Administration of testosterone to ovariectomized females caused activity to decrease to the level of the males. Conversely, administration of estrogen to castrated males resulted in liver enzyme levels similar to those of the females. When the substrates for in vitro methylation were either mixed heterologous tRNA's from Escherichia coli or mixed homologous methyl-deficient tRNA from livers of ethionine-treated rats, the difference in activity between males and females was about 35%. When amino acid-specific tRNA's from E. coli were used as substrates, the ratios of activity of enzymes from females to that of males were: tRNANfMet 1.5; tRNAMetMet 1.1; tRNASer3 1.85; tRNAPhe 1.1; and tRNATyr 1.25, indicating that there are qualitative as well as quantitative differences in the liver tRNA methyltransferases of the two sexes. The adenosylmethionine decarboxylase activity of female rat liver preparations was approximately double that found for males. Testosterone, given to ovariectomized females, lowered the activity of this enzyme to about the same level as that of males. It is not clear whether the observed sex-related differences in activity of several adenosylmethionine-utilizing liver enzymes represent isolated phenomena or are indicative of a sex-related difference in the rate of liver adenosylmethionine turnover.
D012437:Adenosylmethionine Decarboxylase / Q000378:metabolism*; D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D002369:Castration; D005260:Female; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D012737:Sex Factors; D013379:Substrate Specificity; D013739:Testosterone / Q000494:pharmacology; D012359:tRNA Methyltransferases / Q000378:metabolism*
D013739:Testosterone; D012359:tRNA Methyltransferases; D002262:Carboxy-Lyases; D012437:Adenosylmethionine Decarboxylase