title: Identification of an N-acetyl keto derivative of fumonisin B1 in corn cultures of Fusarium proliferatum.
abstract: A method is presented for the separation and identification of a new N-acetyl keto derivative of fumonisin B1 (FB1) produced in solid corn culture. Cultures of Fusarium proliferatum (M-1597) were purified using preparative hplc, and the new fumonisin was detected by negative-ion esms. Structures were confirmed by 1H- and 13C-nmr spectroscopy. The new fumonisin differs from FB1 in that the tricarballylic acid functionality at the C-15 position of the eicosane backbone is replaced by a ketone and the amino group is acetylated. Direct analysis of the culture material by negative-ion electrospray lc/ms confirmed that the new fumonisin is produced naturally by the fungus.
mesh_terms: D000107:Acetylation; D037341:Fumonisins; D005670:Fusarium / Q000737:chemistry* / Q000378:metabolism; D007659:Ketones / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009183:Mycotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D003313:Zea mays / Q000382:microbiology
chemical_list: D037341:Fumonisins; D007659:Ketones; D009183:Mycotoxins; C056933:fumonisin B1