Alterations in erythrocyte band 3 organization induced by the photosensitizer, hematoporphyrin derivative.
Photosensitization of erythrocytes in the presence of hematoporphyrin derivative causes cross-linking of membrane proteins. This cross-linking is associated with partial lysis of the cells and an increased susceptibility to heat-induced membrane fragmentation. The effect of photosensitization on the organization of erythrocyte band 3 was monitored using the technique of time-resolved phosphorescence anisotropy. Band 3 rotational diffusion was somewhat restricted upon photooxidation, indicating aggregation of this major integral membrane protein.
D001457:Anion Exchange Protein 1, Erythrocyte / Q000187:drug effects*; D016880:Anisotropy; D004910:Erythrocyte Membrane / Q000187:drug effects*; D017324:Hematoporphyrin Derivative / Q000494:pharmacology*; D006801:Humans; D049449:Luminescence; D017319:Photosensitizing Agents / Q000494:pharmacology*
D001457:Anion Exchange Protein 1, Erythrocyte; D017319:Photosensitizing Agents; D017324:Hematoporphyrin Derivative