A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate-polyacrylamide gels.
A simple, sensitive silver staining method for sodium dodecyl sulfate polyacrylamide gel electrophoresis has been developed which yields high resolution of proteins with a transparent background. The method includes the incorporation of a water wash after a short fixation step in ethanol/ acetic acid and prior to glutaraldehyde cross-linking, which appears to be necessary for the high resolution staining of protein bands and the low background staining.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D010940:Plant Proteins / Q000032:analysis*; D000461:Rhodophyta / Q000737:chemistry; D012680:Sensitivity and Specificity; D016622:Silver Staining / Q000379:methods*
D010940:Plant Proteins