Assessment of two protocols for the human lymphocyte cytokinesis-blocked micronucleus assay.
This study indicated that by adding cytochalasin B (6 micrograms/ml) at 24 h, the lymphocyte culture time for micronucleus (MN) assay could be shortened to 64 h. In both unirradiated and ex-vivo irradiated (2 Gy) lymphocytes from three populations, we found that the differences in MN yield obtained by our modified cytokinesis-blocked time frame and that recommended by Fenech and Morley (1985) were insignificant (P = 0.66-0.87). We believe that the shorter assay time may enhance the applicability of MN assay for the rapid assessment of ionizing radiation overexposures.
D000704:Analysis of Variance; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D009369:Neoplasms / Q000097:blood / Q000276:immunology* / Q000532:radiotherapy
D009153:Mutagens; D003571:Cytochalasin B