Monoclonal antibody developed against a hemolysin of Bacillus thuringiensis.
A total of five hybridoma cell lines that produced monoclonal antibodies (MAb) against a hemolysin (Bt-hemolysin) produced by Bacillus thuringiensis were established and characterized. All of these monoclonal antibodies reacted similarly not only to Bt-hemolysin but also to a hemolysin (Bc-hemolysin) produced by B. cereus, suggesting that the two hemolysins are immunologically indistinguishable. The MAb developed in this study was also successfully applied for rapid and simple purification of both Bt- and Bc-hemolysins by immunoaffinity column chromatography. The partial N-terminal amino acid sequence of the purified hemolysins was determined to be Ile-Glu-Gln-Thr.
D000911:Antibodies, Monoclonal / Q000276:immunology*; D001413:Bacillus thuringiensis / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000276:immunology*; D006825:Hybridomas
D000911:Antibodies, Monoclonal; D006460:Hemolysin Proteins