[Structural properties of ribosomal protein S8 from the extreme thermophile Thermus thermophilus].
The gene of ribosomal protein S8 from the extreme thermophile Thermus thermophilus was expressed in E. coli using the strain BL21(DE3) and vector pET3-1. A method of isolating this protein from the super producing strain was developed, which makes it possible to obtain 8-12 mg of product from 11 of culture. The secondary structure of protein S8 was determined by using CD spectroscopy. The protein was shown to be highly resistant to denaturants.
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011489:Protein Denaturation; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D013816:Thermodynamics; D016963:Thermus thermophilus / Q000235:genetics / Q000378:metabolism*
D012269:Ribosomal Proteins; C030254:ribosomal protein S8
Strukturnye svoĭstva riboxomnogo belka S8 iz ékstremal'nogo termofila Thermus thermophilus.