Involvement of PIM-1 in DNA fragmentation in mouse NS-1-derived cells.
In several cell lines derived from mouse NS-1 myeloma cells, internucleosomal fragmentation of chromosomal DNA, a hallmark of apoptosis, was continuously observed. Approximately 15-20% of the cells died when isolated in a 96-well plate, and the surviving cells contained fragmented DNA ('ladder'). Among a variety of genes so far reported to be related to apoptosis, only Pim-1 was expressed at an elevated level in the NS-1 hybridomas as compared in a control myeloma cell line without 'ladder'. Transfection of a Pim-1 expression vector to a 'ladder'-non-producing myeloma line yielded similar internucleosomal DNA fragmentation. The results hence suggested that Pim-1 activates endonucleases responsible for DNA fragmentation during apoptosis and/or repress DNA repair systems to restore fragmented DNA.
D000818:Animals; D017209:Apoptosis; D001483:Base Sequence; D002460:Cell Line; D002999:Clone Cells; D004249:DNA Damage; D017931:DNA Primers; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000302:isolation & purification / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression; D006825:Hybridomas; D051379:Mice; D008969:Molecular Sequence Data; D009101:Multiple Myeloma; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000378:metabolism*; D051573:Proto-Oncogene Proteins c-pim-1; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D015342:DNA Probes; D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; C496636:Pim1 protein, mouse; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1