title: Purification of recombinant Rho/Rac/G25K from Escherichia coli.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli; D020558:GTP Phosphohydrolases / Q000096:biosynthesis / Q000302:isolation & purification*; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D005982:Glutathione Transferase / Q000096:biosynthesis; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D008322:Mammals; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification*; D015183:Restriction Mapping; D012549:Schistosoma japonicum / Q000201:enzymology; D020744:rac GTP-Binding Proteins
chemical_list: D018076:DNA, Complementary; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D005982:Glutathione Transferase; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020744:rac GTP-Binding Proteins