Site-specific modifications of light chain glycosylated antilymphoma (LL2) and anti-carcinoembryonic antigen (hImmu-14-N) antibody divalent f1agments.
Site-specific introduction of metal-chelating groups into F(ab')2 fragments of an antilymphoma antibody (LL2) possessing a natural Asn-linked light chain carbohydrate and an anti-carcinoembryonic antigen antibody (hImmu-14-N) grafted with a light chain carbohydrate site is described. For this purpose, four yttrium- (and indium)-chelating agents were used, containing a primary amino group for antibody binding and 1-(4-substituted benzyl)diethylenetriaminepentaacetic acid as the metal-chelator, separated by structurally different additional linkers. Conjugates were prepared by reacting excess chelator with oxidized carbohydrate of F(ab')2 fragments, with or without a subsequent reduction step. The conjugates, with up to an average of 5.5 chelating groups attached to a F(ab')2 fragment, were readily labeled with 90Y and 111In and were found to retain antigen-binding ability in in vitro assays. Tumor targeting was demonstrated using a 88Y-labeled hImmu-14-N F(ab')2 carbohydrate-modified conjugate. 2-Pyridyldithiopropionic hydrazide was conjugated to the carbohydrate region, and the disulfide was selectively deprotected to the thiol group, which is reactive with reduced 99mTc. These initial experiments establish that light chain carbohydrate modification of F(ab')2 is as facile as with the Fc-region carbohydrate of intact IgG, and thereby offer the possibility of designing site-specifically substituted F(ab')2 fragments with favorable pharmacokinetic properties.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000737:chemistry*; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D006031:Glycosylation; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry*; D007147:Immunoglobulin Light Chains / Q000737:chemistry*; D007205:Indium Radioisotopes; D007553:Isotope Labeling; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008819:Mice, Nude; D013667:Technetium; D015021:Yttrium Radioisotopes
D000912:Antibodies, Neoplasm; D002272:Carcinoembryonic Antigen; D007140:Immunoglobulin Fab Fragments; D007147:Immunoglobulin Light Chains; D007205:Indium Radioisotopes; D015021:Yttrium Radioisotopes; D013667:Technetium