Polymorphic CAC/T repetitive sequences in the pig genome 1.
Three genomic clones were isolated from a size-selected pig DNA library by hybridization with a DNA-fingerprint probe. Analysis at the sequence level revealed that all three clones contain interrupted stretches of triplet repeats mainly composed of CAC and CAT triplets. Evaluation of the corresponding loci for polymorphism by Southern blot hybridization showed considerable length variation. For two loci the polymorphism was also demonstrated by polymerase chain reaction (PCR) amplification. The PiGMaP reference pedigree was typed for all three loci.
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016172:DNA Fingerprinting; D015342:DNA Probes; D004251:DNA Transposable Elements; D015723:Gene Library; D016678:Genome; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D013552:Swine / Q000235:genetics*
D015342:DNA Probes; D004251:DNA Transposable Elements