title: The versican C-type lectin domain recognizes the adhesion protein tenascin-R.
abstract: The core proteins of large chondroitin sulfate proteoglycans contain a C-type lectin domain. The lectin domain of one of these proteoglycans, versican, was expressed as a recombinant 15-kDa protein and shown to bind to insolubilized fucose and GlcNAc. The lectin domain showed strong binding in a gel blotting assay to a glycoprotein doublet in rat brain extracts. The binding was calcium dependent and abolished by chemical deglycosylation treatment of the ligand glycoprotein. The versican-binding glycoprotein was identified as the cell adhesion protein tenascin-R, and versican and tenascin-R were both found to be localized in the granular layer of rat cerebellum. These results show that the versican lectin domain is a binding domain with a highly targeted specificity. It may allow versican to assemble complexes containing proteoglycan, an adhesion protein, and hyaluronan.
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002531:Cerebellum / Q000737:chemistry; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics / Q000378:metabolism*; D003429:Cross Reactions; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005643:Fucose / Q000378:metabolism; D006031:Glycosylation; D037102:Lectins / Q000378:metabolism*; D037181:Lectins, C-Type; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D019063:Tenascin / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D053675:Versicans
chemical_list: D015815:Cell Adhesion Molecules; D011508:Chondroitin Sulfate Proteoglycans; D016326:Extracellular Matrix Proteins; D037102:Lectins; D037181:Lectins, C-Type; D009005:Monosaccharides; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D019063:Tenascin; C508324:Vcan protein, rat; D053675:Versicans; C074638:tenascin R; D005643:Fucose; D000117:Acetylglucosamine