Interaction of photodynamically induced cell killing and dark cytotoxicity of rhodamine 123.
Loss of clonogenicity of Chinese hamster ovary (CHO) cells, murine L929 fibroblasts and human bladder carcinoma T24 cells caused by photodynamic treatment (PDT) with hematoporphyrin derivative (HPD) is synergistically enhanced by subsequent incubation with rhodamine 123 in the dark. For CHO and L929 cells this synergistic interaction can be explained by an increased uptake of rhodamine 123 as the result of the photodynamic treatment. With aluminum phthalocyanine (AIPC) as photosensitizer only additive effects were observed in the three cell lines. Incubation in the dark with rhodamine 123, followed by a photodynamic treatment with HPD, resulted in an antagonistic interaction with regard to loss of colony formation. With AIPc the combination of treatments resulted in an additive effect with L929 and T24 cells, whereas with CHO cells a slight antagonistic interaction was observed. An antagonistic effect was also observed in model experiments, treating histidine photodynamically with HPD and measuring oxygen consumption. A possible explanation of these results could be an interaction or complex formation of rhodamine 123 with HPD resulting in a diminished singlet oxygen production. With AIPc this does not take place.
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000493:pharmacokinetics / Q000633:toxicity*; D001692:Biological Transport / Q000187:drug effects; D016466:CHO Cells; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D006224:Cricetinae; D003624:Darkness; D017324:Hematoporphyrin Derivative / Q000494:pharmacology; D006801:Humans; D007739:L Cells; D008027:Light; D051379:Mice; D020112:Rhodamine 123; D012235:Rhodamines / Q000493:pharmacokinetics / Q000633:toxicity*; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms
D000964:Antimetabolites, Antineoplastic; D012235:Rhodamines; D020112:Rhodamine 123; D017324:Hematoporphyrin Derivative