Toxicity of 3-methylindole, 1-nitronaphthalene and paraquat in precision-cut rat lung slices.
The toxicity of 3-methylindole, 1-nitronaphthalene and paraquat has been studied in precision-cut rat lung slice cultures. Lung slices were prepared from male Sprague-Dawley rats using an agarose gel instilling technique with a Krumdieck tissue slicer and cultured for 24 h in a dynamic organ culture system. Treatment of rat lung slices with 3-methylindole, 1-nitronaphthalene or paraquat produced concentration dependent decreases in lung slice protein synthesis and potassium content. EC50 values (concentration to produce a 50% inhibition) for protein synthesis were 0.024, 0.27 and 0.57 mM for paraquat, 1-nitronaphthalene and 3-methylindole, respectively. These results demonstrate that precision-cut lung slices are a useful in vitro model system for studying the pulmonary toxicity of xenobiotics. Lung slices offer the potential as a rapid in vitro screen for identifying pulmonary toxicants and to evaluate species differences in response.
D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D046508:Culture Techniques; D004121:Dimethyl Sulfoxide / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D006540:Herbicides / Q000633:toxicity*; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009281:Naphthalenes / Q000633:toxicity*; D010269:Paraquat / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012862:Skatole / Q000633:toxicity*
D002273:Carcinogens; D006540:Herbicides; D009281:Naphthalenes; D012862:Skatole; C016614:1-nitronaphthalene; D010269:Paraquat; D004121:Dimethyl Sulfoxide