title: Gene 2 of the sigma rhabdovirus genome encodes the P protein, and gene 3 encodes a protein related to the reverse transcriptase of retroelements.
abstract: The nucleotide sequence of the genes 2 and 3 of the Drosophila rhabdovirus sigma was determined from cDNAs to viral genome and poly(A)+ mRNAs. Gene 2 comprises 1032 nucleotides and contains a long ORF encoding a molecular weight 35,208 polypeptide present in infected cells and in virions which migrates in SDS-PAGE as a doublet of M(r) about 60 kDa. The distribution of acidic charges as well as the electrophoretic properties of the protein are characteristic of the rhabdovirus P proteins. Gene 3 comprises 923 nucleotides and contains a long ORF capable of coding a polypeptide of 298 amino acids of MW 33,790. The putative protein (PP3) is similar in size to a minor component of the virions. Computer analysis shows that the sequence of PP3 contains three motifs related to the conserved motifs of reverse transcriptases.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D007304:Insect Viruses / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000032:analysis; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000235:genetics*; D018626:Retroelements / Q000235:genetics; D012191:Retroviridae Proteins / Q000737:chemistry / Q000235:genetics; D012209:Rhabdoviridae / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D009711:Nucleotides; C096912:P protein, Sigma virus; D018626:Retroelements; D012191:Retroviridae Proteins; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase