title: Plasmid ligation using a sacrificial insert.
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004247:DNA / Q000378:metabolism*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D010766:Phosphorylation; D010957:Plasmids
chemical_list: D004247:DNA; C046184:endodeoxyribonuclease PaeI; C034510:CTCGAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific