[Characteristics of hybrid genes coding functionally-active secretory metalloproteinases from bacilli].
A set of different hybrid genes encoding functionally active enzymes was obtained by homologous recombination between the fragments of related Bacillus amyloliquefaciens and Bacillus brevis metalloprotease genes cloned in plasmid vector in tandem orientation. The nucleotide sequences of hybrid genes were analyzed. It was demonstrated that the presence even of short homologous regions is sufficient for effective recombination in Bacillus cells.
D001407:Bacillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D005822:Genetic Vectors; D008666:Metalloendopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D004274:DNA, Recombinant; D011993:Recombinant Fusion Proteins; D008666:Metalloendopeptidases
Kharakteristika gibridnykh genov, kodiruiushchikh funktsional'no- aktivnye sekretornye metalloproteinazy batsill.