Quantitative determination of E- and Z-doxepin and E- and Z-desmethyldoxepin by high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method was developed for the quantitative, simultaneous determination of the following four compounds in serum: E-doxepin, Z-doxepin, E-desmethyldoxepin, and Z-desmethyldoxepin. A 3-microns analytical silica column (6 x 100 mm) was employed with the mobile phase 0.025 M phosphate:acetonitrile:n-nonylamine (80:20:1). This HPLC method allows for the accurate measurement of all four isomeric compounds.
D000929:Antidepressive Agents, Tricyclic / Q000097:blood* / Q000627:therapeutic use; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D004316:Doxepin / Q000031:analogs & derivatives* / Q000097:blood* / Q000737:chemistry / Q000592:standards / Q000627:therapeutic use; D016903:Drug Monitoring; D006801:Humans
D000929:Antidepressive Agents, Tricyclic; D004316:Doxepin; C028701:desmethyldoxepin