Sodium pivalate reduces cardiac carnitine content and increases glucose oxidation without affecting cardiac functional capacity.
This study determined how selected functional, metabolic, and contractile properties were impacted by sodium pivalate, a compound which creates a secondary carnitine deficiency. Young male rats received either sodium pivalate (20 mM, PIV) or sodium bicarbonate (20 mM, CONTR) in their drinking water. After 11-12 weeks cardiac function and glucose oxidation rates were measured in isolated, perfused working heart preparations. Hearts were also analyzed for carnitine content, activities of hexokinase (HK), citrate synthase (CS), and B-hydroxyacyl CoA dehydrogenase (HOAD), and myosin isoenzyme distribution. Sodium pivalate treatment significantly reduced cardiac carnitine content and increased glucose oxidation but did not alter cardiac functional capacity. HK activity was increased in the PIV group (p < 0.05), and HOAD activity decreased (p < 0.05). CS activity and myosin isoform distribution (VI > 85%) remained unchanged. These results demonstrate that pivalate treatment of this duration and the accompanying carnitine deficiency shift cardiac substrate utilization without compromising cardiac functional capacity.
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000818:Animals; D002331:Carnitine / Q000172:deficiency / Q000378:metabolism*; D002950:Citrate (si)-Synthase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D006593:Hexokinase / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism; D010084:Oxidation-Reduction; D010421:Pentanoic Acids / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D007527:Isoenzymes; D010421:Pentanoic Acids; C005566:pivalic acid; D015094:3-Hydroxyacyl CoA Dehydrogenases; D002950:Citrate (si)-Synthase; D006593:Hexokinase; D009218:Myosins; D005947:Glucose; D002331:Carnitine