Long-range restriction mapping of megabase-sized chromosomes that may be homologs in Trypanosoma brucei.
Trypanosoma brucei is a blood-borne pathogen that changes its variant surface glycoprotein coat, thus evading immune destruction. Restriction digestion, combined with probe hybridization studies, was used to construct long-range restriction maps of the 1.4 (M4) and 1.5 megabase (M3) chromosomes from the IsTaR1 serodeme of T. b. brucei. Comparison of the two chromosomes suggests that they are a homologous pair. Hybridization with a repetitive sequence probe also identifies several copies on the M4 chromosome and a relative paucity of cross-hybridizing repetitive sequence on the larger M3 chromosome.
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D016054:DNA, Protozoan / Q000032:analysis* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015345:Oligonucleotide Probes; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000254:growth & development
D016054:DNA, Protozoan; D015345:Oligonucleotide Probes