Fast solute release from photosensitive liposomes: an alternative to 'caged' reagents for use in biological systems.
The kinetics of release of soluble marker trapped in liposomes of gel phase phospholipid containing a photoisomerisable phospholipid analogue have been investigated. Marker release is triggered by UV laser flash photolysis at 355 nm. A markedly temperature-dependent release rate is seen, and above 25 degrees C millisecond release kinetics can be achieved. These results suggest that such liposomes might find application as an alternative to conventional 'caged' reagents for photo-triggered reagent release in biological research.
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D005452:Fluoresceins; D007202:Indicators and Reagents; D007700:Kinetics; D007834:Lasers; D008027:Light; D008081:Liposomes / Q000528:radiation effects*; D008968:Molecular Conformation; D010713:Phosphatidylcholines / Q000737:chemistry*; D010782:Photolysis; D013237:Stereoisomerism; D013997:Time Factors; D014466:Ultraviolet Rays
D005452:Fluoresceins; D007202:Indicators and Reagents; D008081:Liposomes; D010713:Phosphatidylcholines; C053913:1,2-bis(4-(n-butyl)phenylazo-4'-phenylbutyroyl)phosphatidylcholine; D015060:1,2-Dipalmitoylphosphatidylcholine; C007740:fluorexon