Characterization of breakpoint cluster region kinase and SH2-binding activities.
BCR is an interesting signaling protein, whose cellular function is currently unknown. Its biochemical properties include serine kinase activity, SH2-binding activity, and a GTPase-activating activity. The SH2-binding activity is particularly interesting because it may link BCR to signaling pathways involving SH2-containing molecules. Since tyrosine phosphorylation of BCR has been detected in CML-derived cell lines and since tyrosine-phosphorylated BCR shows increased affinity toward certain SH2 domains, it seems particularly important to further characterize this activity. This chapter described a simple purification scheme for partial purification of BCR, which can be used to assess in vitro kinase and SH2-binding activities.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002846:Chromatography, Affinity / Q000379:methods; D002892:Chromosomes, Human, Pair 22; D002899:Chromosomes, Human, Pair 9; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005091:Exons; D020558:GTP Phosphohydrolases / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics; D008715:Methionine / Q000378:metabolism; D015513:Oncogene Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D011505:Protein-Tyrosine Kinases; D011518:Proto-Oncogene Proteins; D051562:Proto-Oncogene Proteins c-bcr; D011865:Radioisotope Dilution Technique; D011994:Recombinant Proteins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D018411:Spodoptera; D013462:Sulfur Radioisotopes; D014162:Transfection / Q000379:methods; D014178:Translocation, Genetic
D015513:Oncogene Proteins; D010761:Phosphorus Radioisotopes; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D013462:Sulfur Radioisotopes; D000255:Adenosine Triphosphate; D008715:Methionine; D011505:Protein-Tyrosine Kinases; C496613:BCR protein, human; D017346:Protein Serine-Threonine Kinases; D051562:Proto-Oncogene Proteins c-bcr; D020558:GTP Phosphohydrolases