Biochemical changes induced by pyrphenoxone in the lens of rabbits and rats.
The xanthomatine analogue, pyrphenoxone, which is known to diminish the incidence of cataract in animals and in man, was applied in two different in vivo models of cataract induced in rabbits by tryptophan-free dietary regimen and in rats by hypergalactosemic diet. The drug was also applied at different concentrations in an in vitro model of cataract. It was found that soluble proteins and sulphurated amino acids of the lens in all in vivo and in vitro models of cataract were higher after pyrphenoxone was applied. Furthermore, the drug treatment was followed by a dose-dependent increase in reduced glutathione content in the lens of rabbits and rats. The same was found in the in vitro model of cataract. These results suggest that pyrphenoxone may act by inducing various biochemical changes that lead to a protection of lens against oxidative processes.
D000818:Animals; D002386:Cataract / Q000188:drug therapy; D004032:Diet; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005123:Eye / Q000187:drug effects*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D008297:Male; D010078:Oxazines / Q000494:pharmacology*; D011725:Pyridines / Q000494:pharmacology*; D011805:Quinolinic Acids / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
D010078:Oxazines; D011725:Pyridines; D011805:Quinolinic Acids; C096543:pyrphenoxone; D005978:Glutathione