title: When cells stop making sense: effects of nonsense codons on RNA metabolism in vertebrate cells.
abstract: It appears that no organism is immune to the effects of nonsense codons on mRNA abundance. The study of how nonsense codons alter RNA metabolism is still at an early stage, and our current understanding derives more from incidental vignettes than from experimental undertakings that address molecular mechanisms. Challenges for the future include identifying the gene products and RNA sequences that function in nonsense mediated RNA loss, resolving the cause and consequences of there apparently being more than one cellular site and mechanism for nonsense-mediated RNA loss, and understanding how these sites and mechanisms are related to both constitutive and specialized pathways of pre-mRNA processing and mRNA decay.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002451:Cell Compartmentation; D018389:Codon, Nonsense; D005246:Feedback; D006207:Half-Life; D008957:Models, Genetic; D008969:Molecular Sequence Data; D012322:RNA Precursors / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D014714:Vertebrates / Q000235:genetics*
chemical_list: D018389:Codon, Nonsense; D012322:RNA Precursors; D012333:RNA, Messenger