Plant virus expressing hybrid coat protein with added murine epitope elicits autoantibody response.
A modified tobacco mosaic virus (TMV) was used to direct the synthesis of TMV coat protein hybrids containing a 13 amino acid sequence of the murine zona pellucida ZP3 protein. This hybrid protein was found to be robust and accumulate to high concentrations in inoculated plants. Virus-like particles containing the hybrid coat protein could be readily purified from infected plant tissue. Parenteral immunization with these virus-like particles as antigen resulted in serum antibody recognizing ZP3 epitope in C57BL/6J and BALB/cBy mice. The antibodies recognized synthetic ZP3 peptide and the authentic murine ZP3 glycoprotein. Antibodies were recruited in vivo to the zone pellucida in female mice.
D000818:Animals; D000941:Antigens / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D001483:Base Sequence; D002213:Capsid / Q000276:immunology; D004527:Egg Proteins / Q000276:immunology*; D000939:Epitopes; D005260:Female; D007114:Immunization; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014614:Vaccines, Synthetic / Q000276:immunology*; D015044:Zona Pellucida / Q000276:immunology*; D000072216:Zona Pellucida Glycoproteins
D000941:Antigens; D001323:Autoantibodies; D004527:Egg Proteins; D000939:Epitopes; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D000072216:Zona Pellucida Glycoproteins; C000606521:Zp3 protein, mouse