Establishment of a monoepoxide (leukotoxin and its isomer) producing system using a hydrogen peroxide-generating system.
We established an effective monoepoxide-generating system by combining cytochrome-c (Cyt-c) with a hydrogen peroxide (H2O2)-generating system comprising hypoxanthine (HX), xanthine oxidase (XO) and superoxide dismutase (SOD; HX-XO-SOD-Cyt-c system). Using this and the H2O2-Cyt-c system, we proved that monoepoxide production from linoleic acid was due to hydroxy radical formation by the reaction of Cyt-c with H2O2 and not to the formation of other active oxygen species.
D001565:Benzoates; D019817:Benzoic Acid; D003574:Cytochrome c Group; D004852:Epoxy Compounds / Q000138:chemical synthesis* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D008353:Mannitol; D009603:Nitroso Compounds; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D019820:Xanthine; D014969:Xanthine Oxidase; D014970:Xanthines
D001565:Benzoates; D003574:Cytochrome c Group; D004852:Epoxy Compounds; D007202:Indicators and Reagents; D009603:Nitroso Compounds; D017382:Reactive Oxygen Species; D014970:Xanthines; C008651:4-nitrosodimethylaniline; D019820:Xanthine; D008353:Mannitol; D019817:Benzoic Acid; D006861:Hydrogen Peroxide; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase