Decreased cysteine and proline synthesis in parenterally fed, premature infants.
Little is known about the amino acid (AA) biosynthetic capacity and requirements of premature infants. This study assessed the synthesis of seven biochemically nonessential AA from a universal precursor, glucose, in stable, parenterally fed, premature neonates. Seven infants (six boys, one girl) were studied at a mean age of 6.3 +/- 0.6 (SEM) days; mean gestational age was 29.7 +/- 1.3 (SEM) weeks, and mean birth weight was 1,222.8 +/- 176.5 (SEM) grams. All infants were parenterally fed a mixture of 7.5% to 12.5% dextrose and 2.2% Trophamine, with or without lipid. Mean caloric intake was 93 +/- 8.4 (SEM) kcal/kg/d, and total AA intake was standardized at 2.86 g/kg/d AA, plus supplemental cysteine (30 mg/g AA/d). Each infant received a 4-hour continuous, unprimed intravenous infusion of a stable isotope tracer of D(-)[U13C] glucose (200 mg/kg). Blood samples were obtained before and at the end of the infusion. Conversion of the glucose tracer into seven biochemically nonessential AA (cysteine [Cys], proline [Pro], aspartate [Asp], serine [Ser], glutamate [Glu], alanine [Ala], and glycine [Gly]) was assessed by measuring their isotopic enrichment in plasma, using gas chromatography/mass spectrometry (GC/MS), and expressed as mole percent excess (MPE) (mean +/- SEM). The isotopic enrichment of plasma glucose was also measured using GC/MS. Free plasma AA concentrations (mean +/- SD) were measured using an automated amino acid analyzer. Mean MPE for M + 1, M + 2 and M + 3 Cys, and for M + 1 and M + 3 Pro were not significantly different from 0; M + 2 Pro barely achieved statistical significance (P = .048).(ABSTRACT TRUNCATED AT 250 WORDS)
D000409:Alanine / Q000096:biosynthesis / Q000097:blood; D000596:Amino Acids / Q000008:administration & dosage; D001224:Aspartic Acid / Q000096:biosynthesis / Q000097:blood; D001724:Birth Weight; D001786:Blood Glucose / Q000032:analysis; D002247:Carbon Isotopes; D003545:Cysteine / Q000096:biosynthesis* / Q000097:blood; D004573:Electrolytes; D002149:Energy Intake; D005260:Female; D005526:Food, Formulated; D008401:Gas Chromatography-Mass Spectrometry; D005865:Gestational Age; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism; D018698:Glutamic Acid / Q000096:biosynthesis / Q000097:blood; D005998:Glycine / Q000096:biosynthesis / Q000097:blood; D006801:Humans; D007225:Infant Food; D007227:Infant Nutritional Physiological Phenomena; D007231:Infant, Newborn; D007234:Infant, Premature / Q000097:blood / Q000378:metabolism*; D008055:Lipids / Q000008:administration & dosage; D008297:Male; D010288:Parenteral Nutrition; D057947:Parenteral Nutrition Solutions; D011392:Proline / Q000096:biosynthesis* / Q000097:blood; D012694:Serine / Q000096:biosynthesis / Q000097:blood; D012996:Solutions
D000596:Amino Acids; D001786:Blood Glucose; D002247:Carbon Isotopes; D004573:Electrolytes; D008055:Lipids; D057947:Parenteral Nutrition Solutions; D012996:Solutions; C545824:amino-acid, glucose, and electrolyte solution; D001224:Aspartic Acid; D018698:Glutamic Acid; D012694:Serine; D011392:Proline; D005947:Glucose; D003545:Cysteine; D000409:Alanine; D005998:Glycine