Novel sensing system for glucose based on the complex formation between phenylborate and fluorescent diol compounds.
From the standpoints of long-term stability and simple handling, a glucose sensor composed of totally synthetic sensing moieties should be of great advantage. This study was performed to demonstrate the feasibility of a novel glucose sensing system in an aqueous milieu based on a change in the fluorescence on competitive binding between a fluorescent diol compound and glucose toward a phenylborate compound. 6,7-Dihydroxy-4-methyl-coumarin (DHMC) was selected as a fluorophore, and 3-propionamidophenylboronic acid (PAPBA) as a phenylborate compound. The relationship between the glucose concentration and fluorescence intensity was linear in the range of 0 to 500 mg/dl of glucose. The availability of various derivatives of DHMC and phenylborate compounds allows the incorporation of these functionalities on the tip of an optical fiber to construct an opto-sensing system for glucose.
D001897:Boronic Acids / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D015336:Molecular Probe Techniques; D015394:Molecular Structure; D012995:Solubility; D013050:Spectrometry, Fluorescence; D014468:Umbelliferones / Q000737:chemistry*
D001897:Boronic Acids; D014468:Umbelliferones; C096881:3-propionamidophenylboronic acid; C096882:6,7-dihydroxy-4-methylcoumarin; D005947:Glucose