Expression of type I interleukin-1 receptor mRNA and its regulation in cultured astrocytes.
The expression of type I interleukin-1 receptor (IL-1RI) mRNA enriched astrocytes and microglia was investigated by Northern blot analysis. IL-1RI mRNA was expressed in untreated astrocytes, but not in microglia. This expression was markedly suppressed by a treatment with isoproterenol, a beta-adrenoceptor agonist (1 x 10(-8) to 1 x 10(-6) M). This suppression was clearly reversed by propranolol, a beta-adrenoceptor antagonist. Dibutyryl cAMP (dbcAMP) also suppressed the expression of IL-1RI mRNA. The suppression by isoproterenol or dbcAMP was blocked by H8 or H89, inhibitors of cAMP-dependent protein kinase. The present results suggest that IL-1RI mRNA expression in cultured astrocytes may be regulated by intracellular cAMP, elevated upon the activation of beta-adrenoceptors as an example, and cAMP-dependent protein kinase.
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D015152:Blotting, Northern; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D004396:Coloring Agents; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000187:drug effects; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D013268:Stimulation, Chemical; D013778:Tetrazolium Salts; D013844:Thiazoles
D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D004396:Coloring Agents; D007375:Interleukin-1; D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D003994:Bucladesine; D003513:Cycloheximide; D011433:Propranolol; D017868:Cyclic AMP-Dependent Protein Kinases; C022616:thiazolyl blue; D007545:Isoproterenol