Determination of strychnine and brucine by capillary zone electrophoresis.
A procedure for quantitative estimation of strychnine and brucine in the extracts of Strychnos nux-vomica seeds by capillary zone electrophoresis (CZE) was developed. The buffer solution used was 10mM phosphate buffer-MeOH (9:1), pH 2.5. The linear calibration range was 0.01-0.15 mg/ml. This method is useful for the qualitative and quantitative determination of strychnine and brucine in plant drug samples, as well as in human plasma.
D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005240:Feasibility Studies; D006801:Humans; D012639:Seeds / Q000737:chemistry; D013331:Strychnine / Q000031:analogs & derivatives* / Q000032:analysis* / Q000097:blood
D004365:Drugs, Chinese Herbal; C083806:brucine; D013331:Strychnine