Simultaneous determination of bestatin and p-hydroxybestatin, a major metabolite, in human serum by gas chromatography mass spectrometry. Utilization of quantitative selected ion monitoring and deuterium labelled internal standard.
A specific and sensitive method for the simultaneous determination of bestatin and its major metabolite, p-hydroxybestatin in human serum has been developed by selected ion monitoring using the corresponding deuterated internal standards. The N-trifluoroacetamide-O-trimethylsilyl either derivatives of the trideuteromethyl esters of bestatin and p-hydroxybestatin were suitable for quantitation by selected ion monitoring. The determination limit of these compounds by the present method is 1 ng ml-1 in human serum.
D000328:Adult; D003903:Deuterium; D004791:Enzyme Inhibitors / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007930:Leucine / Q000031:analogs & derivatives* / Q000097:blood; D008875:Middle Aged
D004791:Enzyme Inhibitors; C029246:4-hydroxybestatin; D003903:Deuterium; D007930:Leucine