Actin polymerization induced by GTP gamma S in permeabilized neutrophils is induced and maintained by free barbed ends.
To address the mechanisms through which agonists stimulate actin polymerization, we examined the roles of monomer sequestering proteins and free barbed ends on actin polymerization induced by guanosine 5'-3-O-(thio)triphosphate (GTP gamma S) in neutrophils permeabilized with streptolysin O. Addition of profilin (without GTP gamma S) caused a net decrease in F-actin. Thus, merely making profilin available in the cell was not sufficient to induce actin polymerization. On the other hand, addition of profilin hardly affected the polymerization induced by GTP gamma S, while thymosin beta 4 or DNase I decreased this polymerization. These data suggested that GTP gamma S induced polymerization by increasing the availability of barbed ends. In the presence of cytochalasin B, profilin did inhibit polymerization induced by GTP gamma S, demonstrating that GTP gamma S did not inhibit profilin's monomer sequestering ability. The F-actin induced by GTP gamma S was not limited by a time-dependent loss of G-actin or G-proteins from permeabilized cells since, following stimulation with suboptimal concentrations of GTP gamma S, addition of more GTP gamma S induced further polymerization. Barbed ends remained free after F-actin reached plateau since (a) cytochalasin B caused depolymerization of induced F-actin and (b) profilin did not depolymerize induced F-actin unless the cells were first treated with cytochalasin to cap barbed ends. The data indicate that GTP gamma S maintains an increased level of F-actin by keeping at least a few barbed ends available for polymerization.
D000199:Actins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins; D002463:Cell Membrane Permeability; D003571:Cytochalasin B / Q000494:pharmacology; D003850:Deoxyribonuclease I / Q000494:pharmacology; D005456:Fluorescent Dyes; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008433:Mathematics; D009504:Neutrophils / Q000502:physiology*; D010590:Phalloidine / Q000031:analogs & derivatives; D011817:Rabbits; D012235:Rhodamines; D013301:Streptolysins; D013947:Thymosin / Q000494:pharmacology; D013997:Time Factors; D000099201:Thymosin beta(4)
D000199:Actins; D001426:Bacterial Proteins; D003571:Cytochalasin B; D003850:Deoxyribonuclease I; D005456:Fluorescent Dyes; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D010590:Phalloidine; D012235:Rhodamines; D013301:Streptolysins; D013947:Thymosin; D000099201:Thymosin beta(4); C025964:streptolysin O; C041085:tetramethylrhodaminylphalloidine