[Identification of procollagen mRNA in chronic active hepatitis B].
In situ hybridization is a tool for staining intracellular procollagen mRNAs with specific probes. Our study shows the amounts of procollagen mRNAs of types I and III to be increased in liver biopsies of five patients with chronic active hepatitis B as compared with five healthy controls. Parallel staining employing anti-smooth-muscle-actin antibodies was able to identify myofibroblast-like cells at the same localization where procollagen mRNAs were found. Consequently, these transformed Ito-cells might be the procollagen-producing cells.
D000199:Actins / Q000235:genetics; D000328:Adult; D000368:Aged; D001706:Biopsy; D005260:Female; D006509:Hepatitis B / Q000235:genetics* / Q000473:pathology; D006521:Hepatitis, Chronic / Q000235:genetics* / Q000473:pathology; D006801:Humans; D017403:In Situ Hybridization; D008099:Liver / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011347:Procollagen / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*
D000199:Actins; D011347:Procollagen; D012333:RNA, Messenger
Identifikation von Prokollagen-mRNAs bei chronisch aktiver Hepatitis B.