Mutation detection using immobilized mismatch binding protein (MutS).
An accurate and highly sensitive mutation detection assay has been developed. The assay is based on the detection of mispaired and unpaired bases by immobilized mismatch binding protein (Escherichia coli MutS). The assay can detect most mismatches and all single base substitution mutations, as well as small addition or deletion mutations. The assay is simple to use and does not require the use of either radioactivity or gel electrophoresis.
D000251:Adenosine Triphosphatases; D000755:Anemia, Sickle Cell / Q000235:genetics; D001426:Bacterial Proteins; D001482:Base Composition; D001483:Base Sequence; D004252:DNA Mutational Analysis / Q000379:methods*; D004260:DNA Repair; D004268:DNA-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D005914:Globins / Q000235:genetics; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008163:Luminescent Measurements; D008969:Molecular Sequence Data; D051719:MutS DNA Mismatch-Binding Protein; D009692:Nucleic Acid Heteroduplexes; D016133:Polymerase Chain Reaction
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D009692:Nucleic Acid Heteroduplexes; D005914:Globins; D000251:Adenosine Triphosphatases; D051719:MutS DNA Mismatch-Binding Protein; C497175:MutS protein, E coli