Identification of the Ulex europaeus agglutinin-I-binding protein as a unique glycoform of the neural cell adhesion molecule in the olfactory sensory axons of adults rats.
Histochemical localization of two lectins, Ulex europaeus agglutinin-I (UEA-I) and Tetragonolobus purpureus (TPA), was studied in the olfactory bulb of adult rats. In contrast to TPA, UEA-I detected a fucosylated glycoprotein that is only present in the surface membranes of olfactory sensory cells including the whole course of their neurites up to the final arborization in glomeruli. Immunoblotting revealed that UEA-I binds specifically to a protein of 205 kDa, while TPA stains several other glycoproteins. Affinity chromatography with the use of a UEA-I column identified the 205 kDa protein as a glycoform of neural cell adhesion molecule (N-CAM), specific for the rat olfactory sensory nerves.
D000373:Agglutinins / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000737:chemistry*; D015153:Blotting, Western; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis* / Q000378:metabolism; D037102:Lectins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D008970:Molecular Weight; D009830:Olfactory Bulb / Q000166:cytology; D018034:Olfactory Receptor Neurons / Q000737:chemistry* / Q000648:ultrastructure; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley
D000373:Agglutinins; D015816:Cell Adhesion Molecules, Neuronal; D037102:Lectins; D008562:Membrane Glycoproteins