title: Viability of fat obtained by syringe suction lipectomy: effects of local anesthesia with lidocaine.
abstract: The results of transplantation of free autologous fat obtained by blunt syringe suction lipectomy are unpredictable. We examined if adipose tissue viability is compromised by using syringe suction lipectomy and by infiltration of the tissue with local anesthetics. As reference, we used adipose tissue samples excised during elective surgery. Fat obtained intraoperatively and by lipectomy was digested with collagenase to isolate adipocytes. The mechanical damage associated with sample handling and cell isolation in both procedures was similar and did not exceed 6% of the total cell mass. In addition, cells isolated from intraoperative and lipectomy samples did not differ functionally, responded similarly to insulin stimulation of glucose transport and epinephrine-stimulated lipolysis, and retained the same growth pattern in culture. Since during fat transplantation the graft is exposed to local anesthetics at both the donor and the recipient sites, we reexamined adipocyte function in the presence of lidocaine. Lidocaine potently inhibited glucose transport and lipolysis in adipocytes and their growth in culture. That effect, however, persisted only as long as lidocaine was present; after washing, the cells were able to fully regain their function and growth regardless of whether the exposure was as short as 30 minutes or as long as 10 days. These results indicate that adipose tissue obtained by syringe lipectomy consists of fully viable and functional adipocytes, but local anesthetics may halt their metabolism and growth.
mesh_terms: D017667:Adipocytes / Q000187:drug effects* / Q000502:physiology / Q000637:transplantation; D000779:Anesthetics, Local / Q000009:adverse effects*; D002470:Cell Survival; D005260:Female; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007279:Injections, Subcutaneous; D008012:Lidocaine / Q000009:adverse effects*; D015187:Lipectomy / Q000379:methods*; D008066:Lipolysis; D008297:Male; D008875:Middle Aged; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods
chemical_list: D000779:Anesthetics, Local; D008012:Lidocaine; D005947:Glucose