Prolonged blood circulation in rats of nanospheres surface-modified with semitelechelic poly[N-(2-hydroxypropyl)methacrylamide].
Semitelechelic poly[N-(2-hydroxypropyl)methacrylamide]s (ST-PHPMA) containing one amino end-group and differing in molecular weight were synthesized by radical polymerization in the presence of 2-aminoethanethiol (AET) as chain transfer agent. These polymers were covalently attached via amide bonds to the surface of nanospheres based on a copolymer of methyl methacrylate, maleic anhydride, and methacrylic acid. When compared to unmodified nanospheres, those with the surface modified with ST-PHPMA possessed a decreased protein (albumin, IgG, fibrinogen) adsorption in vitro, an increased intravascular half-life as well as a decreased accumulation in the liver after intravenous administration into rats. The higher the molecular weight of the ST-PHPMA, the more pronounced the changes in these properties. The results obtained have clearly demonstrated that covalently attached ST-PHPMA chains are efficient in decreasing the biorecognition of negatively charged (hydrophilic) polymer surfaces.
D000180:Acrylic Resins / Q000138:chemical synthesis / Q000378:metabolism* / Q000493:pharmacokinetics; D000818:Animals; D001775:Blood Circulation / Q000502:physiology*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005069:Evaluation Studies as Topic; D006207:Half-Life; D008099:Liver / Q000378:metabolism; D008299:Maleic Anhydrides / Q000737:chemistry; D008689:Methacrylates / Q000737:chemistry; D020366:Methylmethacrylate; D008768:Methylmethacrylates / Q000737:chemistry; D008863:Microspheres; D012157:Mononuclear Phagocyte System / Q000378:metabolism; D010316:Particle Size; D011108:Polymers / Q000138:chemical synthesis / Q000493:pharmacokinetics; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000378:metabolism* / Q000493:pharmacokinetics; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000378:metabolism; D013499:Surface Properties
D000180:Acrylic Resins; D008299:Maleic Anhydrides; D008689:Methacrylates; D008768:Methylmethacrylates; D011108:Polymers; D011109:Polymethacrylic Acids; D011506:Proteins; D020366:Methylmethacrylate; C008384:methacrylic acid; C011533:Duxon