Collagen matrix: an attractive alternative to agar and methylcellulose for the culture of hematopoietic progenitors in autologous transplantation products.
Autografts using untreated or in vitro manipulated bone marrow and peripheral blood stem cells represent promising approaches to the treatment of malignant diseases. In this work, the collagen gel culture technique was compared with agar and methylcellulose for its capacity to permit the growth of human granulomonocytic (day 14 CFU-GM; collagen vs agar or MTC) or erythroblastic (day 7 CFU-E and day 14 BFU-E; collagen versus methylcellulose) colonies in autologous transplantation products. Our results show that the collagen culture system always gave as many or more colonies than the other techniques. It also allowed harvesting of gels onto glass slides and subsequent May-Grünwald-Giemsa, cytochemical or immunocytochemical staining. We suggest that the collagen assay represents an interesting alternative to the widely used agar or methylcellulose systems for the culture of hematopoietic progenitors because of the equal or higher number of colonies detected, the easy phenotypical identification of colonies in stained gels, and the ability to store high-quality documentation. This technique is particularly attractive for use in the quality control of autologous bone marrow transplantation procedures.
D000362:Agar; D016026:Bone Marrow Transplantation / Q000592:standards; D003094:Collagen; D003114:Colony-Forming Units Assay; D004396:Coloring Agents; D046508:Culture Techniques / Q000379:methods*; D004900:Erythroblasts / Q000166:cytology / Q000473:pathology; D005109:Extracellular Matrix; D006098:Granulocytes / Q000166:cytology / Q000473:pathology; D018380:Hematopoietic Stem Cell Transplantation / Q000592:standards; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008747:Methylcellulose; D011786:Quality Control; D013194:Staining and Labeling; D014182:Transplantation, Autologous
D004396:Coloring Agents; D000362:Agar; D008747:Methylcellulose; D003094:Collagen