Reduction of acetylcholine-activated current by low concentrations of extracellular adenosine 5'-triphosphate.
Effects of adenosine 5'-triphosphate (ATP) on ionic currents activated by acetylcholine (ACh) were investigated using rat pheochromocytoma PC12 cells and Xenopus oocytes expressing nicotinic receptors. In PC12 cells, ATP (10 nM to 1 microM) inhibited an inward current activated by ACh in not all but about 60% of cell batches. The ACh-activated current was also inhibited by ATP in Xenopus oocytes and, with a subunit combination of alpha 3 plus beta 4, the inhibition was observed at concentration as low as 100 fM. Uridine 5'-triphosphate (UTP) induced a similar inhibition of the ACh-activated current both in PC12 cells and Xenopus oocytes. These and other properties suggest that the current reduction by ATP is not mediated through conventional P2-purinoceptors.
D000109:Acetylcholine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D005260:Female; D007473:Ion Channels / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D014544:Uridine Triphosphate / Q000494:pharmacology; D014981:Xenopus
D007473:Ion Channels; D000255:Adenosine Triphosphate; D000109:Acetylcholine; D014544:Uridine Triphosphate