Comparison of three human-murine heteromyeloma cell lines for formation of human hybridomas after electrofusion with human peripheral blood lymphocytes from meningococcal cases and carriers.
Peripheral blood lymphocytes from meningitis patients and healthy meningococcal carriers were fused by electrofusion with the three human-murine heteromyeloma cell lines CB-F7, K6H6B5 and H7NS. 934 hybridomas producing human immunoglobulins were obtained in 30 fusions. Heteromyeloma K6H6B5 yielded a significantly higher proportion of hybridomas producing IgG antibodies than did the two other cell lines. CB-F7 and K6H6B5 yielded comparable numbers of hybridomas whose supernatants reacted with homologous bacteria, whereas the cell line H7NS was less efficient.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D002459:Cell Fusion; D002460:Cell Line; D006801:Humans; D006825:Hybridomas / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology; D008585:Meningitis, Meningococcal / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal