title: Properties of the Kv1.1 rat brain potassium channels expressed in mammalian cells: temperature effects.
abstract: We studied the temperature dependence of the currents mediated by potassium channels Kv1.1 cloned from rat brain expressed by permanent transfection in the human embryonic kidney cell line 292er. Voltage-gated outward currents, with a reversal potential near to the Nernst potential for potassium, were elicited by depolarising pulses. The currents activate fast and do not show inactivation during 50-ms test pulses. Increasing temperature decreased the amplitude of the currents, slowed the kinetics of activation and deactivation and shifted the activation curve to more negative potentials. We conclude that in Kv1.1 channels the closed states are associated with more ordered structure of the channel protein than the open state. The voltage-dependence and the kinetic properties are similar to those expressed in frog oocytes injected with cRNA coding the same Kv1.1.
mesh_terms: D000818:Animals; D001001:Anura; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D005260:Female; D006801:Humans; D015640:Ion Channel Gating; D007668:Kidney; D007700:Kinetics; D051662:Kv1.1 Potassium Channel; D008322:Mammals; D008564:Membrane Potentials; D009865:Oocytes / Q000502:physiology; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000096:biosynthesis / Q000502:physiology*; D024642:Potassium Channels, Voltage-Gated; D018075:RNA, Complementary; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D013696:Temperature; D014162:Transfection
chemical_list: C496946:KCNA1 protein, human; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D018075:RNA, Complementary; D011994:Recombinant Proteins; D051662:Kv1.1 Potassium Channel