beta-Galactoside alpha-2,3-sialyltransferase messenger RNA increases in thyrotrophs of hypothyroid mice.
Our goal was to learn about the cellular mechanism(s) responsible for the increased sialylation of thyroid-stimulating hormone (TSH) during hypothyroidism. We used in situ hybridization to examine the beta-galactoside alpha-2,3-sialyltransferase mRNA content in thyrotrophs and corticotrophs of euthyroid and hypothyroid [propylthiouracil (PTU)-treated] mice. The alpha-2,3-sialyltransferase mRNA level was 66, 70, 296, and 223% higher in hypothyroid thyrotrophs than in euthyroid thyrotrophs after mice received PTU for 1, 2, 3, and 6 weeks, respectively. Increased transcription of this gene, or mRNA stabilization, may partially explain the increased sialylation of TSH during hypothyroidism.
D000818:Animals; D001483:Base Sequence; D015342:DNA Probes; D007037:Hypothyroidism / Q000201:enzymology*; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid; D012333:RNA, Messenger / Q000378:metabolism*; D012794:Sialic Acids / Q000378:metabolism; D012799:Sialyltransferases / Q000235:genetics*; D013961:Thyroid Gland / Q000201:enzymology*; D013972:Thyrotropin / Q000378:metabolism; D013974:Thyroxine / Q000097:blood; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
D015342:DNA Probes; D012333:RNA, Messenger; D012794:Sialic Acids; D013972:Thyrotropin; D012799:Sialyltransferases; D019158:N-Acetylneuraminic Acid; D013974:Thyroxine; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase