[The effect of 5-fluorouracil on the clonogenic hematopoietic cells (CFU-S) of embryonic and sexually mature mice].
We studied sensitivity of various types of clonogenic hemopoietic cells (CFU-S-7, CFU-S-11, and CFU-ep) on the liver of 14-days embryos and from the bone marrow and spleen of adult mice to the cytotoxic agent 5-fluorouracil (5-FU) in vitro and in vivo. We discovered that different types of CFU-S of the embryos and adult mice has similar sensitivity to 5-FU in vitro. At the same time in vivo the bone marrow CFU-S-7 display a higher sensitivity to 5-FU than CFU-S-11, thus agreeing with the published data (Hodgson and Bradley, 1979). The differences between the 5-FU effects in vitro and in vivo are related to the hemopoietic microenvironment, which modulates the cytotoxic 5-FU effect in vivo.
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002470:Cell Survival / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004305:Dose-Response Relationship, Drug; D004622:Embryo, Mammalian; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D008099:Liver / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011247:Pregnancy; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013997:Time Factors
D000964:Antimetabolites, Antineoplastic; D005472:Fluorouracil
Vliianie 5-ftoruratsila na klonogennye krovetvornye kletki (KOE-S) zarodysheĭ i polovozrelykh mysheĭ.