Improved genomic/nuclear DNA extraction for 8-hydroxydeoxyguanosine analysis of small amounts of rat liver tissue.
Using two different commercially available extraction kits, genomic/nuclear DNA could be recovered from rat liver samples as small as 10 mg. Background 8-hydroxydeoxyguanosine levels in such DNA were low and stable at 0.26-0.30 +/- 0.01-0.03/10(5) guanine residues. The minimum tissue wet weight required for the accurate 8-hydroxydeoxyguanosine analysis was 25 mg. The present results indicate that routine and reliable assessment of the involvement of oxidative DNA damage in the development of various diseases, including cancer, is feasible using a variety of tissue sources.
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000302:isolation & purification*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis; D004563:Electrochemistry; D008099:Liver / Q000737:chemistry*; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344
D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine