title: Structure specific ds/ss-RNase activity in the extreme halophile Halobacterium salinarium.
abstract: A ds/ss-RNA processing activity involved in antisense-RNA mediated gene regulation in the extremely halophilic archaebacterium Halobacterium salinarium was investigated in vivo. H.salinarium cells were transformed with DNA encoding an RNA species complementary to a part of the major lytic transcript, termed T4, of the H.salinarium phage phi H. The transformants transcribing this construct, when infected by phage were able to process T4 in a similar way to the processing of the lytic transcript denoted T1, in the natural sense-antisense system. Processing of T4 was not observed under normal phage growth on wild-type cells. Thus the antisense-RNA mediated processing activity earlier reported is dependent on the presence of an RNA duplex and is not sequence specific.
authors.affiliation: Maz-Planck-Institut für Biochemie, Martinsried, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Stolt
authors.forename: W
authors.initials: W
authors.lastname: Zillig
mesh_terms: D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D004269:DNA, Bacterial; D004279:DNA, Viral; D015964:Gene Expression Regulation, Bacterial; D006217:Halobacterium / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D012323:RNA Processing, Post-Transcriptional; D016372:RNA, Antisense / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D016372:RNA, Antisense; D012329:RNA, Bacterial; D012330:RNA, Double-Stranded; D012260:Ribonucleases