Human platelet antigen-1 (Zw) typing of fetuses by analysis of polymerase chain reaction-amplified genomic DNA from amniocytes.
Prenatal typing for the human platelet antigens-1 (HPA) permits identification of a fetus at risk for neonatal alloimmune thrombocytopenia (NAITP) in cases of HPA-1 incompatibility in which the father is heterozygous for the HPA-1a antigen. Diagnostic cordocentesis and phenotyping of the fetal platelets are used for this purpose. We applied allele-specific restriction enzyme analysis on polymerase chain reaction (PCR)-amplified DNA purified from amniocytes. This assays allows early second trimester typing for HPA-1 alleles. We were able to determine the genotype of three fetuses at risk. Iatrogenic fetal loss is lower with amniocentesis than with cordocentesis. Therefore, this technique is a welcome addition to the antenatal management of NAITP.
Simsek
S
S
Department of Immunological Haematology, University of Amsterdam, The Netherlands.
Christiaens
G C
GC
Kanhai
H H
HH
Beekhuis
J R
JR
Bleeker
P M
PM
Vlekke
A B
AB
Goldschmeding
R
R
von dem Borne
A E
AE
D000483:Alleles; D000649:Amniocentesis; D000653:Amniotic Fluid / Q000166:cytology*; D016824:Antigens, Human Platelet / Q000235:genetics / Q000276:immunology*; D001788:Blood Grouping and Crossmatching; D002478:Cells, Cultured; D017218:Cordocentesis; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D006801:Humans; D007114:Immunization; D039661:Integrin beta3; D007518:Isoantibodies / Q000097:blood*; D008297:Male; D010375:Pedigree; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D011296:Prenatal Diagnosis; D012306:Risk; D012449:Safety; D013921:Thrombocytopenia / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*
D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3; D007518:Isoantibodies; D004247:DNA; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific