Characterisation of putative monoclonal anti-G3m(u) and anti-G3m(g) reagents and their antigenic determinants.
Monoclonal antibodies (MAbs) against IgG3 allotypic markers were evaluated in haemagglutination inhibition and IgG3 capture ELISA. MAbs PNF69C and 200D1 exhibited G3m(u) and G3m(g) specificity respectively. In HAI target epitopes detected by MAbs were remarkably stable to physiochemical degradation. Western blotting revealed that MAb 200D1, bound to intact IgG3 heavy chain disease protein and not its pFc' fragment; a result consistent with the CH2 domain location of the G3m(g) allotope. The G3m(u) allotope is also located within this domain. Surprisingly anti-G3m(u) MAb PNF69C bound to the pFc' of IgG3-related protein, HW, and to the pFc' of IgG1-related protein, PR, in Western blot.
Nelson
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PN
Division of Immunology, St. George's Hospital Medical School, London, U.K.
Goodall
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M
Jefferis
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D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D006362:Heavy Chain Disease / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007074:Immunoglobulin G / Q000145:classification / Q000276:immunology*; D007129:Immunoglobulin Gm Allotypes / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D007129:Immunoglobulin Gm Allotypes; D007143:Immunoglobulin Heavy Chains