IL-4 production in mediastinal lymph node cells in mice intratracheally instilled with diesel exhaust particulates and antigen.
To clarify the relationship between air pollutants and IgE antibody production, interleukin 4 (IL-4) production was investigated in BALB/c mice intratracheally injected with diesel exhaust particulates (DEP) mixed with antigen (Ovalbumin (OA) or Japanese Cedar Pollen (JCP)). BALB/c mice were injected with DEP plus OA or OA alone three times with a 3-week interval. After the last instillation, proliferative response and lymphokine-producing activity of mediastinal lymph node cells (LNC) were examined in vitro. Proliferative response to OA in mediastinal LNC from mice injected with DEP plus OA was enhanced 4-17 times of that from control mice. IL-4-producing activity by OA stimulation also enhanced in mediastinal LNC from mice injected with DEP plus OA. A significantly larger amount of anti-OA IgE antibody was detected in sera from DEP- and OA-injected mice compared with those from control mice. The levels of IL-4, estimated by JCP antigen in mediastinal LNC, from mice injected with DEP plus JCP were two-fold higher than those from mice injected with JCP alone. These results suggest that intratracheal instillation of DEP affects antigen-specific IgE antibody responses via local T-cell activation, especially enhanced IL-4 production.
Fujimaki
H
H
National Institute for Environmental Studies Onogawa, Ibaraki, Japan.
Nohara
O
O
Ichinose
T
T
Watanabe
N
N
Saito
S
S
D000818:Animals; D000941:Antigens / Q000276:immunology*; D002478:Cells, Cultured; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis*; D007442:Intubation, Intratracheal; D008198:Lymph Nodes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008297:Male; D008482:Mediastinum; D051379:Mice; D008807:Mice, Inbred BALB C; D010323:Passive Cutaneous Anaphylaxis; D011058:Pollen / Q000276:immunology; D001335:Vehicle Emissions / Q000633:toxicity*
D000941:Antigens; D007376:Interleukin-2; D001335:Vehicle Emissions; D015847:Interleukin-4; D007073:Immunoglobulin E