Co-mutagenic effect of tannic acid on ring-X chromosome loss induced by mitomycin C in sperm cells of Drosophila melanogaster.
To investigate the effects of tannic acid (TA) on ring-X chromosome loss, Drosophila melanogaster females exposed to different TA concentrations were crossed with untreated, methyl methanesulfonate (MMS)- or mitomycin C (MMC)-treated males which carried a ring-X chromosome. Progeny were analyzed for loss of the ring-X. The results of this in vivo study showed that TA had no suppressing effect on chromosome loss occurring spontaneously or after induction by MMS in mature spermatozoa. In contrast, TA caused a significant increase in the frequency of MMC-induced ring-X loss. The increase caused by this co-mutagenic effect reached values of 34, 33 and 40% at TA concentrations of 10, 25 and 50 mM, respectively. These increments may reflect the action of TA on a uvrABC-type enzyme which, by increasing the double-strand breaks (DSBs), somehow interferes with the post-replicational repair responsible for the final DSB correction.
Cunha
K S
KS
Departamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
Reguly
M L
ML
Gimmler-Luz
M C
MC
Santos
J H
JH
Lehmann
M
M
de Andrade
H H
HH
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology; D016009:Chi-Square Distribution; D002872:Chromosome Deletion; D004260:DNA Repair / Q000187:drug effects; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D004357:Drug Synergism; D005260:Female; D047348:Hydrolyzable Tannins / Q000633:toxicity*; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity; D016685:Mitomycin / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D012303:Ring Chromosomes; D013094:Spermatozoa / Q000187:drug effects; D014960:X Chromosome / Q000187:drug effects*
D016587:Antimutagenic Agents; D047348:Hydrolyzable Tannins; D009153:Mutagens; D016685:Mitomycin; D008741:Methyl Methanesulfonate