title: The single pseudouridine residue in Escherichia coli 16S RNA is located at position 516.
abstract: The number and location of pseudouridine residues in Escherichia coli 16S ribosomal RNA has been determined by a combination of direct and indirect methods. Only one residue was found, at position 516. This site is at the 5'-end of one of the three most highly conserved long sequences of this RNA molecule. A number of experimental findings have strongly implicated this loop in the fidelity of codon recognition by A-site bound tRNA. By virtue of its location, we suggest that psi 516 may also play a role in maintaining the fidelity of protein synthesis.
authors.affiliation: Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.
authors.forename: A
authors.initials: A
authors.lastname: Bakin
authors.forename: J A
authors.initials: JA
authors.lastname: Kowalak
authors.forename: J A
authors.initials: JA
authors.lastname: McCloskey
authors.forename: J
authors.initials: J
authors.lastname: Ofengand
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011560:Pseudouridine / Q000032:analysis* / Q000235:genetics; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000737:chemistry* / Q000235:genetics
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011560:Pseudouridine