Whole body and splanchnic leucine, phenylalanine, and glucose kinetics during endotoxemia in humans.
To examine the whole body and splanchnic tissue substrate handling during endotoxemia, an intravenous bolus of endotoxin was given to six healthy volunteers during primed, continuous infusions of [1-13C]leucine, [ring-2H5]phenylalanine, and [6,6-2H2]glucose. Whole body protein breakdown, based on whole body Leu and Phe appearance rates (Ra), increased in response to endotoxin given at time 0 (RaLeu 77 +/- 2 mol.kg-1 x h-1, t = 0 h; 88 +/- 6, t = 4 h; P < 0.05) (RaPhe 39 +/- 2 mol.kg-1 x h-1, t = 0; 46 +/- 3, t = 4 h; P < 0.05). Splanchnic amino acid balance (Bal) increased (BalLeu 7 +/- 4 mol.kg-1 x h-1, t = 0; 21 +/- 5, t = 2 h; P < 0.05) (BalPhe 3 +/- 2 mol.kg-1 x h-1, t = 0; 16 +/- 4, t = 2 h; P < 0.05) and can be accounted for by increased splanchnic uptake (Rd) of Phe and Leu (RdLeu 21 +/- 3 mol.kg-1 x h -1, t = 0; 37 +/- 7, t = 120 min; P < 0.05) (RdPhe 10 +/- 3 mol.kg-1 x h-1, t = 0; 24 +/- 5, t = 120 min; P < 0.05). Splanchnic conversion of Leu to ketoisocaproate increased with endotoxin administration (0.7 +/- 0.6 mol.kg-1 x h-1, t = 0; 8 +/- 3, t = 360 min; P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Fong
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Department of Surgery, New York Hospital-Cornell Medical Center, New York 10021.
Matthews
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He
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Marano
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Moldawer
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Lowry
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D000209:Acute-Phase Proteins / Q000378:metabolism; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002247:Carbon Isotopes; D003903:Deuterium; D004731:Endotoxins / Q000633:toxicity*; D004926:Escherichia coli; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D007930:Leucine / Q000097:blood / Q000378:metabolism*; D008297:Male; D010649:Phenylalanine / Q000097:blood / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D013152:Splanchnic Circulation; D014115:Toxemia / Q000097:blood / Q000378:metabolism*
D000209:Acute-Phase Proteins; D001786:Blood Glucose; D002247:Carbon Isotopes; D004731:Endotoxins; D011506:Proteins; D010649:Phenylalanine; D003903:Deuterium; D007930:Leucine; D005947:Glucose