Rapid quantitation of hemoglobin S by isoelectric focusing.
The feasibility of isoelectric focusing (IEF) to determine hemoglobin S (HbS) at a faster turn-around-time and to resolve the HbS and hemoglobin A (HbA) in presence of high concentrations of hemoglobin F (HbF) is evaluated. The IEF procedure is faster, and the results can be obtained in less than 45 minutes. The resulting data are comparable to gel electrophoresis. It is a superior procedure in resolving both HbS and HbA in the presence of high HbF and, therefore, a desirable technique to use for infants and children. Further, IEF is simpler than the gel electrophoresis, relatively inexpensive, easily adaptable for routine use, and suitable for "stat" conditions.
Reddy
M N
MN
Department of Pathology, Medical College of Wisconsin, Milwaukee.
Franciosi
R A
RA
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005319:Fetal Hemoglobin / Q000032:analysis; D006441:Hemoglobin A / Q000032:analysis; D006451:Hemoglobin, Sickle / Q000032:analysis*; D006801:Humans; D007223:Infant; D007525:Isoelectric Focusing
D006451:Hemoglobin, Sickle; D006441:Hemoglobin A; D005319:Fetal Hemoglobin