Quantitative micro P30 and reverse transcriptase assays for Moloney murine leukemia virus.
An anti-P30 immunohistochemical and a reverse transcriptase assay for Moloney murine leukemia virus (MoMLV) are adapted to 96-well plates. The assay results are shown to be directly proportional to the number of infectious particles, and can therefore be used to estimate the infective titers of a virus preparation. The micro P30 assay yields a direct estimate of infectious centers, and the reverse transcriptase assay quantitates progeny from a single cycle of replication. The semi-automated nature of these assays is well suited to the analysis of a large number of samples and therefore permits the examination of the efficiency of the process of retroviral/MoMLV infection under varied times or conditions.
Wilson
C A
CA
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD 20892.
Eiden
M V
MV
Marsh
J W
JW
D016475:3T3 Cells / Q000821:virology; D000818:Animals; D015925:Cryopreservation; D051379:Mice; D008979:Moloney murine leukemia virus / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase / Q000032:analysis*; D015203:Reproducibility of Results; D015682:Retroviridae Proteins, Oncogenic / Q000032:analysis* / Q000276:immunology; D012449:Safety; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000032:analysis* / Q000276:immunology; D010948:Viral Plaque Assay
D015682:Retroviridae Proteins, Oncogenic; D014759:Viral Envelope Proteins; C060711:gp30 envelope transmembrane protein, Bovine leukemia virus; D012194:RNA-Directed DNA Polymerase