title: Peptide bond specificity of calpain: proteolysis of human myelin basic protein.
abstract: In order to determine the peptide bond specificity of calpain, human myelin basic protein (HMBP) was treated with purified calpain of bovine brain. Upon incubation, HMBP component I (HMBP-I) was degraded into several peptides as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Component I was more susceptible to degradation than components II and III. HMBP degradation products were separated by high performance liquid chromatography (HPLC) and the cleavage sites in HMBP molecules were determined by peptide sequence analysis and by N- and C-terminal analyses. The major cleavage site was found to be 94Val-95Thr with several minor cleavages at 49Arg-50Gly, 18Ala-19Ser, 23His-24Ala, 27Gly-28Phe, 59Asp-60Ser, 70Gly-71Ser, 97Arg-98Thr, 110Ser-111Leu, 145Asp-146Ala, and 156Leu-157Gly. These results indicate that calpain is involved in the limited proteolysis of human myelin basic protein and prolonged incubation causes further digestion of the large peptides.
authors.affiliation: Department of Neurology, Medical University of South Carolina, Charleston 29425.
authors.forename: N L
authors.initials: NL
authors.lastname: Banik
authors.forename: C H
authors.initials: CH
authors.lastname: Chou
authors.forename: G E
authors.initials: GE
authors.lastname: Deibler
authors.forename: H C
authors.initials: HC
authors.lastname: Krutzch
authors.forename: E L
authors.initials: EL
authors.lastname: Hogan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000201:enzymology; D002154:Calpain / Q000378:metabolism*; D002417:Cattle; D006801:Humans; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; D002154:Calpain