title: KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria.
abstract: To further elucidate the mechanism of organelle transport, we cloned a novel member of the mouse kinesin superfamily, KIF1B. This N-terminal-type motor protein is expressed ubiquitously in various kinds of tissues. In situ hybridization revealed that KIF1B is expressed abundantly in differentiated nerve cells. Interestingly, K1F1B works as a monomer, having a microtubule plus end-directed motility. Our rotary shadowing electron microscopy revealed mostly single globular structures. Immunocytochemically, KIF1B was colocalized with mitochondria in vivo. Furthermore, a subcellular fractionation study showed that KIF1B was concentrated in the mitochondrial fraction, and purified K1F1B could transport mitochondria along microtubules in vitro. These data strongly suggested that KIF1B works as a monomeric motor for anterograde transport of mitochondria.
authors.affiliation: Department of Anatomy and Cell Biology School of Medicine, University of Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nangaku
authors.forename: R
authors.initials: R
authors.lastname: Sato-Yoshitake
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: Y
authors.initials: Y
authors.lastname: Noda
authors.forename: R
authors.initials: R
authors.lastname: Takemura
authors.forename: H
authors.initials: H
authors.lastname: Yamazaki
authors.forename: N
authors.initials: N
authors.lastname: Hirokawa
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001370:Axonal Transport / Q000502:physiology*; D001692:Biological Transport / Q000502:physiology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018507:Gene Expression Regulation, Developmental; D016547:Kinesins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008928:Mitochondria / Q000502:physiology*; D008969:Molecular Sequence Data; D009068:Movement / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D017433:Protein Structure, Secondary; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: C490398:Kif1b protein, mouse; C490399:Kif1b protein, rat; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins; D016547:Kinesins