Flagellum-mediated adhesion of Trypanosoma congolense to bovine aorta endothelial cells.
We studied the interaction between Trypanosoma congolense and bovine aorta endothelial (BAE) cell monolayers. Our findings suggest that trypanosomes adhere predominantly to the flattened, peripheral cell surface domains as well as to filamentous endothelial outgrowths that are present during in vitro cultivation in non-confluent monolayers. Adhesion is mediated exclusively by the flagellum in a distinct geometrical order with respect to the flagellar cytoskeleton. Thus, it is possible to define exactly the trypanosomal cell surface domain involved in the attachment process. After 24-48 h of cultivation on monolayers, trypanosomes start to develop short, filopodia-like flagellar protrusions, which serve as additional elements in assisting parasite attachment. Small filaments (3-5 nm) also serve as cross-links between flagellar and endothelial cell surface membranes. Lectin-gold labeling shows that these cross-links contain sialic acid residues. In vitro assays confirm that sialic acid is involved in the adhesion process, whereas the extracellular matrix (ECM) proteins fibronectin, collagen, laminin and vitronectin are not. The presence of T. congolense exhibits a mitogenic effect on BAE cells.
Hemphill
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A
Institute for Parasitology, University of Bern, Switzerland.
Ross
C A
CA
D000268:Adhesiveness; D000818:Animals; D001011:Aorta; D002417:Cattle; D002455:Cell Division; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000469:parasitology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005353:Fibronectins / Q000494:pharmacology; D005407:Flagella / Q000378:metabolism* / Q000648:ultrastructure; D007797:Laminin / Q000494:pharmacology; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009077:Mucins / Q000494:pharmacology; D019158:N-Acetylneuraminic Acid; D012794:Sialic Acids / Q000378:metabolism*; D013363:Submandibular Gland; D014348:Trypanosoma congolense / Q000378:metabolism* / Q000648:ultrastructure; D019096:Vitronectin / Q000494:pharmacology
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D009077:Mucins; D012794:Sialic Acids; D019096:Vitronectin; D003094:Collagen; D019158:N-Acetylneuraminic Acid