Human umbilical cord blood CD34+ cell purification with high yield of early progenitors.
Human umbilical cord blood CD34+ cells were purified using a two-step procedure by elimination of the soybean agglutinin-binding cells and by a positive panning selection with a CD34 monoclonal antibody. The isolated fraction was 88-97% pure CD34+ cells. A yield of 48.5% was obtained when comparing the number of cells recovered in the CD34(+)-purified fraction and the number of CD34+ cells detected in the initial mononuclear cell fraction. By flow cytometry, we observed that the CD34+ cells that were not recovered were those that had the lower expression of CD34 antigen and were therefore the more mature cells. A high recovery of CFU-GEMM progenitors (73.9%) was also observed. These data suggest the possibility of purifying CD34+ umbilical cord blood cells for clinical applications, in particular for umbilical cord blood banking.
Cardoso
A A
AA
Laboratoire de Biologie Cellulaire et Moléculaire des Facteurs de Croissance, Centre National de la Recherche Scientifique UPR 272, Villejuif, France.
Li
M L
ML
Batard
P
P
Sansilvestri
P
P
Hatzfeld
A
A
Levesque
J P
JP
Lebkowski
J S
JS
Hatzfeld
J
J
D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology; D018952:Antigens, CD34; D002452:Cell Count; D002469:Cell Separation / Q000379:methods*; D003114:Colony-Forming Units Assay; D005240:Feasibility Studies; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006412:Hematopoietic Stem Cells; D006801:Humans; D007163:Immunosorbent Techniques; D007231:Infant, Newborn; D037102:Lectins; D037121:Plant Lectins; D011994:Recombinant Proteins / Q000494:pharmacology; D030262:Soybean Proteins; D010835:Phytohemagglutinins
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018952:Antigens, CD34; D016298:Hematopoietic Cell Growth Factors; D037102:Lectins; D037121:Plant Lectins; D011994:Recombinant Proteins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins