title: Levels of serum granulocyte colony-stimulating factor in patients with chronic myeloid leukemia.
abstract: To clarify the patho-physiologic role of granulocyte colony-stimulating factor (G-CSF) in chronic myeloid leukemia (CML), we determined the serum levels of G-CSF in various stages of CML using a very sensitive method: chemiluminescence enzyme immunoassay (CLEIA). This method makes it possible to estimate very low levels of serum G-CSF. In the present study, serum samples from 25 patients in chronic phase and 16 in blastic crisis, as well as samples from 33 healthy volunteers were investigated. The serum G-CSF levels in chronic phase of CML (2.95 +/- 3.91 pg/ml) were significantly lower than those in normal controls (15.92 +/- 6.53 pg/ml) and in blastic crisis of CML (15.52 +/- 17.65 pg/ml) within a range of very low levels (p < 0.001, p < 0.02). Moreover, a reverse correlation between blood neutrophil counts and serum G-CSF levels were clearly demonstrated for CML including blastic crisis (r = 0.405, p < 0.02). Interestingly, a sequential parallel relation was observed between serum G-CSF levels and neutrophil alkaline phosphatase (NAP) scores for a patient with CML in chronic phase. Our observations indicate that a negative feedback mechanism exists between peripheral neutrophils and serum G-CSF levels in the chronic phase of CML, and that very low levels of G-CSF in chronic phase of CML might be an important cause for the low NAP scores.
authors.affiliation: First Department of Internal Medicine, Niigata University School of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Saitoh
authors.forename: A
authors.initials: A
authors.lastname: Shibata
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood*; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D016898:Interferon-alpha / Q000494:pharmacology; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood*; D009504:Neutrophils / Q000201:enzymology
chemical_list: D016898:Interferon-alpha; D016179:Granulocyte Colony-Stimulating Factor; D000469:Alkaline Phosphatase; D006918:Hydroxyurea