Increased numbers of circulating basophils with decreased releasability after administration of rhG-CSF to allergic patients.
Preliminary studies in hematological patients have indicated that treatment with rhG-CSF reduces basophil releasability ex vivo. We examined this phenomenon further, in allergic patients. Ten patients with grass pollen rhinoconjunctivitis were given rhG-CSF (5 micrograms/kg/day s.c.) for 5 days, and examined before and after treatment. Basophil counts increased from 5 to 19 x 10(9)/l (P < 0.01). Total blood histamine increased from 80 to 160 micrograms/l (P < 0.01), corresponding to a decrease in average basophil histamine content from 1.5 to 0.81 pg/cell (P < 0.01). Isolated mononuclear cells showed a significantly decreased histamine release (HR) when stimulated with A23187 and grass. Whole blood experiments showed a similar decreased HR to grass and anti-IgE (P < 0.01). However, we found an increase in total blood histamine. We conclude that treatment with rhG-CSF (1) increases the number of circulating blood basophils, (2) reduces the average histamine content per basophil, and (3) reduces the basophil releasability. These findings could be due to the mobilization of immature basophils from the bone marrow.
Pedersen
M
M
University Clinic for Infectious Diseases M, Rigshospitalet, Copenhagen, Denmark.
Kristensen
K S
KS
Clementsen
P
P
Olsen
O T
OT
Skov
P S
PS
Permin
H
H
Norn
S
S
D000328:Adult; D001491:Basophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D000001:Calcimycin / Q000494:pharmacology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D066298:In Vitro Techniques; D007958:Leukocyte Count / Q000187:drug effects*; D008875:Middle Aged; D011058:Pollen / Q000276:immunology; D011994:Recombinant Proteins / Q000494:pharmacology; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology / Q000473:pathology
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D000001:Calcimycin