Detection of Ehrlichia chaffeensis in human tissue by using a species-specific monoclonal antibody.
A mouse monoclonal antibody (MAb 1A9) was produced and used in detection of Ehrlichia chaffeensis in human tissues including kidney, liver, and lung by using an indirect immunohistologic stain. MAb 1A9 was specific to E. chaffeensis and did not react with other bacteria, including Ehrlichia canis, which is the organism most closely related to E. chaffeensis. It reacted with an epitope present in two surface proteins of E. chaffeensis with molecular masses of 29 and 27 kDa. E. chaffeensis was easily detected in human tissue by immunohistology with MAb 1A9. This study demonstrates that our MAb can provide a specific and simple method for detection of E. chaffeensis in clinical specimens for establishing an etiologic diagnosis of human ehrlichiosis; it may also provide a tool for the investigation of immunopathologic characteristics in infected patients.
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Unité des Rickettsies, Faculté de Médecine, Marseille, France.
Brouqui
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Dumler
J S
JS
Raoult
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D000818:Animals; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western; D004536:Ehrlichia / Q000276:immunology* / Q000302:isolation & purification* / Q000648:ultrastructure; D016873:Ehrlichiosis / Q000175:diagnosis* / Q000382:microbiology; D000939:Epitopes / Q000737:chemistry; D005260:Female; D006801:Humans; D007668:Kidney / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes