title: Dietary supplementation with taurine and niacin prevents the increase in lung collagen cross-links in the multidose bleomycin hamster model of pulmonary fibrosis.
abstract: Taurine and niacin have been previously found to block the accumulation of collagen in lung in the multidose bleomycin hamster model of pulmonary fibrosis. Previous studies have found an increase in the pulmonary collagen cross-links dihydroxylysinonoroleucine (DHLNL) and hydroxypyridinium (OHP) in the single dose bleomycin rat model. In this study, we asked if taurine and niacin would block the increase in DHLNL and OHP in the multidose bleomycin hamster model of lung fibrosis. Hamsters were intratracheally instilled with three consecutive doses of saline or bleomycin sulfate 1 week apart (2.5, 2.0, 1.5 units/5 mL/kg). Animals were fed diet containing either 2.5% niacin and 2.5% taurine or control diet throughout the experiment. The four groups were saline-instilled with control diet (SCD), bleomycin instilled with control diet (BCD), bleomycin-instilled with taurine-niacin in diet (BTN), and saline-instilled with taurine-niacin in diet (STN). Animals were sacrificed at 1, 4, and 8 weeks after the last bleomycin instillation. Hydroxyproline per lung in the BCD group was significantly elevated by 38, 56, and 60% over the SCD group at 1, 4, and 8 weeks, respectively. There were no statistically significant differences among the four groups in DHLNL (mmole) per mole collagen at the 1 or 8 week time point. At four weeks, DHLNL was significantly elevated by 46.4% in the BCD group over the SCD group. The OHP (mmole) per mole of collagen at 1 and 4 weeks in the BCD group was not statistically different from the SCD group.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, University of California, Davis 95616.
authors.forename: R J
authors.initials: RJ
authors.lastname: Blaisdell
authors.forename: M J
authors.initials: MJ
authors.lastname: Schiedt
authors.forename: S N
authors.initials: SN
authors.lastname: Giri
mesh_terms: D000818:Animals; D001761:Bleomycin; D003094:Collagen / Q000187:drug effects / Q000378:metabolism*; D006224:Cricetinae; D004032:Diet; D004151:Dipeptides / Q000378:metabolism; D004195:Disease Models, Animal; D008297:Male; D008647:Mesocricetus; D009525:Niacin / Q000008:administration & dosage / Q000494:pharmacology*; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism; D013654:Taurine / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D004151:Dipeptides; D011725:Pyridines; D001761:Bleomycin; C007428:5,5'-dihydroxylysylnorleucine; D013654:Taurine; D009525:Niacin; C023500:3-hydroxypyridine; D003094:Collagen