Constitutive production of IL-6 in the anemic mice of W/Wv genotype.
IL-6 was found to be produced by bone marrow cell fraction enriched for hemopoietic stem cells in response to IL-3 stimulation. To confirm the production of IL-6 by hemopoietic stem cells, we studied the response of W/Wv mouse bone marrow cells to IL-3 stimulation in terms of IL-6 production. Genetically anemic mice of W/Wv genotype are known to be depleted of normal hemopoietic stem cells. Bone marrow cells of W/Wv mice produce IL-6 without IL-3 stimulation. But bone marrow cells of the litter mate (+/+) mice studied as controls do not produce IL-6 without stimulation. IL-3 induced the production of IL-6, GM-CSF and G-CSF from the bone marrow cells of +/+ mice. Gene expression of IL-6, GM-CSF, G-CSF and IL-3 in these mice was detected in total bone marrow cells after IL-3 stimulation. In contrast, bone marrow cells of C3H mice did not produce IL-3 even after IL-3 stimulation. Bone marrow cells of C3H mice reportedly produce a diffusible IL-3 inhibitor (NIL-3), but those of C57BL mice do not. Present findings indicate the strain-dependent differences in hemopoietic stimulating factor production. The constitutive IL-6 production in W/Wv mice might be due to a constant and strong demand for blood cell production of the anemic mice.
Yanai
T
T
Department of Biology, Faculty of Science, Niigata University, Japan.
Sugimoto
K
K
Tsuji
T
T
Takashita
E
E
Mori
K J
KJ
D000740:Anemia / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D005246:Feedback; D005838:Genotype; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D007377:Interleukin-3 / Q000096:biosynthesis / Q000494:pharmacology; D015850:Interleukin-6 / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D007377:Interleukin-3; D015850:Interleukin-6; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor