title: Expression of cytokine-dependent adhesion molecules in postreperfusion biopsy specimens of liver allografts.
abstract: OBJECTIVE
  Allogeneic recognition of donor cells by host T lymphocytes requires the expression of cytokine-dependent molecules, such as class II major histocompatibility antigens, intracellular adhesion molecule 1 (ICAM-1), and lymphocyte function-associated antigen 3 (LFA-3). In the liver, activation of Kupffer cells after ischemic injury during the transplantation procedure may result in an early induction of cytokine-dependent molecules.
  
  
  METHODS
  The pattern of induction of ICAM-1, HLA-DR, HLA-DQ, and LFA-3 was investigated in 30 postreperfusion surgical biopsy specimens of liver allografts by an immunohistochemical technique.
  
  
  RESULTS
  Two patterns of induction were observed: focal or diffuse. On hepatocytes, ICAM-1 was induced in 22 cases (11 focal, 11 diffuse), HLA-DR in 18 cases (13 focal, 5 diffuse), HLA-DQ in 13 cases (3 focal, 10 diffuse), and LFA-3 in 1 case (focal). On bile duct cells, HLA-DR was expressed in 19 cases, associated with HLA-DQ in 7 cases. No induction of ICAM-1 and LFA-3 was detected. Compared with the other patients, the group of patients with diffuse postoperative hepatocellular induction of ICAM-1 was characterized by higher preharvesting serum transaminase levels in the donor (P < or = 0.001), suggestive of preoperative ischemic injury, and increased incidence of acute graft rejection (P = 0.04).
  
  
  CONCLUSIONS
  Preoperative warm ischemia may modify the immunogenicity of liver allografts.
authors.affiliation: Laboratoire de Biologie Cellulaire, Faculté de Médecine Xavier Bichat, Paris, France.
authors.forename: J Y
authors.initials: JY
authors.lastname: Scoazec
authors.forename: F
authors.initials: F
authors.lastname: Durand
authors.forename: C
authors.initials: C
authors.lastname: Degott
authors.forename: D
authors.initials: D
authors.lastname: Delautier
authors.forename: J
authors.initials: J
authors.lastname: Bernuau
authors.forename: J
authors.initials: J
authors.lastname: Belghiti
authors.forename: J P
authors.initials: JP
authors.lastname: Benhamou
authors.forename: G
authors.initials: G
authors.lastname: Feldmann
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000378:metabolism; D001706:Biopsy; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D016207:Cytokines / Q000494:pharmacology*; D005260:Female; D006084:Graft Rejection; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008102:Liver Circulation; D016031:Liver Transplantation; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D008875:Middle Aged; D015424:Reperfusion; D012189:Retrospective Studies; D014184:Transplantation, Homologous
chemical_list: D015703:Antigens, CD; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules; D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D008562:Membrane Glycoproteins; D018799:Intercellular Adhesion Molecule-1