Monocyte differentiation and accessory function: different effects on the proliferative responses of an autoreactive T cell clone as compared to alloreactive or antigen-specific T cell lines and primary mixed lymphocyte cultures.
An autoreactive T cell clone derived from a patient with reactive arthritis, two alloreactive T cell lines, two antigen-specific T cell lines and allogeneic resting T cells were analyzed for their responses to monocytes and macrophages derived from monocytes by in vitro differentiation. The autoreactive T cell clone strongly proliferated in response to fresh monocytes and to macrophages derived from a 7 day culture, but only poorly to monocytes cultured for 2 days. In contrast, alloreactive and antigen-specific T cell lines proliferated to all stimulatory cells equally well. Finally, primary mixed lymphocyte reactions could be stimulated by both fresh and 2-day cultured monocytes, but not by in vitro derived macrophages. The impaired response of the autoreactive T cell clone to 2-day cultured monocytes could not be attributed to reduced expression of several well-defined surface molecules nor to induction of nonresponsiveness. Neither allogeneic monocytes nor cytokines (IL-1, IL-2, IL-4, IL-6) could correct the defective response of the autoreactive T cell clone. However, preculture of monocytes in the presence of interferon-gamma, IL-1, IL-4 or IL-6 retained their stimulatory capacity. Our interpretation of the selectively impaired response of the autoreactive T cell clone is that it most likely recognizes a differentiation-dependent monocyte/macrophage-specific peptide.
Schlesier
M
M
Abteilung Rheumatologie, Medizinische Klinik, Freiburg, Germany.
Krause
S
S
Dräger
R
R
Wolff-Vorbeck
G
G
Kreutz
M
M
Andreesen
R
R
Peter
H H
HH
D000938:Antigen-Presenting Cells / Q000276:immunology; D000954:Antigens, Surface / Q000096:biosynthesis; D001324:Autoantigens / Q000276:immunology*; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007519:Isoantigens / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000954:Antigens, Surface; D001324:Autoantigens; D000939:Epitopes; D007519:Isoantigens