[The study of intermediate filament organization of highly and poorly differentiated nasopharyngeal carcinoma cell lines].
By employing cytochemistry, immunocytochemistry, selective double extraction, whole mount electron microscopy and electrophoretic scanning quantitative analysis, the intermediate filaments (IF) of in vitro cultured nasopharyngeal carcinoma cell lines CNE-1 (highly differentiated) and CNE-2Z (poorly differentiated) have been studied. The results demonstrated that although the keratin-type IF were organized in a network pattern in both kinds of cells, significant differences could be observed between the two kinds of cells regarding IF organization, subtypes and quantity of keratin. The poorly differentiated cell line CNE-2Z exhibited a far more irregular and confused IF organization structure, and the content of keratin was lower while the variety of keratin subtypes was increased.
Ma
W
W
Institute of Basic Medical Sciences, CAMS, Beijing.
D002277:Carcinoma / Q000473:pathology*; D006801:Humans; D007382:Intermediate Filaments / Q000737:chemistry / Q000648:ultrastructure*; D007633:Keratins / Q000032:analysis; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D007633:Keratins