title: Isobutyramide, an orally bioavailable butyrate analogue, stimulates fetal globin gene expression in vitro and in vivo.
abstract: Butyrate and other short-chain fatty acids stimulate fetal globin gene expression and have potential for ameliorating the beta globin disorders. Butyrate, however, is rapidly metabolized in vivo and reaches only micromolar concentrations in plasma. We report here that a branched-chain derivative of butyrate, isobutyramide, increases gamma globin gene expression in cultured human erythroid progenitors in vitro and stimulates activity from a minimal gamma globin gene promoter linked to a reporter gene in stable and transient expression assays, with slightly less activity in these in vitro assays than butyrate. In vivo, administration of isobutyramide to anaemic adult baboons rapidly stimulates fetal globin synthesis and F-reticulocyte production. Plasma concentrations at millimolar levels are achieved after a single intravenous or oral dose (500-600 mg/kg), and these concentrations are maintained for 9.5-10.5 h. These results indicate that although isobutyramide has slightly less activity than butyrate in vitro in enhancing fetal globin expression at the cellular and molecular level, its prolonged in vivo half-life may provide superior activity as a therapeutic agent for reactivating fetal globin gene expression in vivo.
authors.affiliation: Cancer Research Center, Boston University School of Medicine, Massachusetts 02118.
authors.forename: S P
authors.initials: SP
authors.lastname: Perrine
authors.forename: G H
authors.initials: GH
authors.lastname: Dover
authors.forename: P
authors.initials: P
authors.lastname: Daftari
authors.forename: C T
authors.initials: CT
authors.lastname: Walsh
authors.forename: Y
authors.initials: Y
authors.lastname: Jin
authors.forename: A
authors.initials: A
authors.lastname: Mays
authors.forename: D V
authors.initials: DV
authors.lastname: Faller
mesh_terms: D000284:Administration, Oral; D000577:Amides / Q000378:metabolism / Q000494:pharmacology*; D000755:Anemia, Sickle Cell / Q000097:blood*; D000818:Animals; D015672:Erythroid Precursor Cells / Q000502:physiology*; D005319:Fetal Hemoglobin / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects*; D017930:Genes, Reporter; D005914:Globins / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008297:Male; D010215:Papio; D011401:Promoter Regions, Genetic; D014162:Transfection; D017086:beta-Thalassemia / Q000097:blood
chemical_list: D000577:Amides; C030197:isobutyramide; D005914:Globins; D005319:Fetal Hemoglobin