Antibody response to the 89-kDa outer membrane protein of Brucella in bovine brucellosis.
The antibody response of cattle to the minor 89-kDa outer-membrane protein (OMP) of brucella was measured by indirect ELISA with the purified protein and compared with the antibody response to smooth lipopolysaccharide (S-LPS). Pre-incubating sera with sonicated cell extracts of Escherichia coli prevented the binding of antibodies from uninfected animals to the 89-kDa OMP, suggesting the presence of one or more cross-reactive epitopes on this protein. In cattle infected experimentally with Brucella abortus, the antibody response to the 89-kDa OMP was later and less intense than that to S-LPS. In naturally infected cattle, 68% of animals showing an antibody response to S-LPS also showed an antibody response to the 89-kDa OMP. Results indicate that specific epitopes of the 89-kDa OMP in combination with those of other OMPs could be useful for diagnosis of brucellosis in cattle.
Limet
J N
JN
Facultes Universitaires Notre Dame de la Paix, Namur, Belgium.
Cloeckaert
A
A
Bezard
G
G
Van Broeck
J
J
Dubray
G
G
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000911:Antibodies, Monoclonal / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology* / Q000302:isolation & purification; D002003:Brucella abortus / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D016622:Silver Staining
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D008070:Lipopolysaccharides