Expression of substance P and its precursor forms in vagal, tracheal, and lung tissues of the guinea pig.
Steady-state levels of the prototypic tachykinin neuropeptide substance P (SP) and its major precursor form substance P-glycine (SP-G) were detected and authenticated in guinea pig vagal and respiratory tissues by radioimmunoassay (RIA), combined high-performance liquid chromatography (HPLC)/RIA analyses, and immunohistochemistry. Four antisera were employed: anti-SP that recognizes the amidated COOH-terminal of SP and is specific for the mature peptide, anti-SP4-10 that recognizes the midportion 4-10 amino acid sequence of SP and is highly specific for both mature SP and extended precursor forms of SP, anti-SP-G that is highly specific for the unamidated COOH-terminal of SP-G, and affinity-purified anti-SP-G-K that is capable of detecting SP-G and minor forms of SP precursor in immunohistochemical analyses. In all examined areas, the content of substance P4-10-like immunoreactivity (SP4-10-LI) quantified by RIA with the use of anti-SP4-10 was greater than that quantified by RIA with the use of anti-SP serum, thereby providing biochemical evidence of steady-state expression of extended precursor forms of SP. Immunohistochemical analyses demonstrated labeled axonal profiles indicating the presence of immunoreactive SP as well as immunoreactive forms of SP precursor within lung hilum and in small fibers in the parenchyma, with no evidence of labeled neuronal cell bodies in these same areas.
Kream
R M
RM
Department of Anesthesiology, Tufts University School of Medicine, Boston, Massachusetts.
Marchand
J E
JE
O'Connor
S T
ST
Bloomquist
E I
EI
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008168:Lung / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000032:analysis*; D011817:Rabbits; D011863:Radioimmunoassay; D013373:Substance P / Q000032:analysis*; D014132:Trachea / Q000737:chemistry*; D014630:Vagus Nerve / Q000737:chemistry*
D011498:Protein Precursors; D013373:Substance P