LGL-1: a potential triggering molecule on murine NK cells.
Natural killer (NK) cells mediate non-major histocompatibility complex-restricted lysis of tumor cells, lymphokine-activated killing (LAK), antibody-dependent cellular cytotoxicity (ADCC), and reverse ADCC (RADCC). LGL-1+ cells identify a major subset (50%) of murine NK cells. Here we demonstrate that monoclonal antibodies (mAbs) to LGL-1 consistently induce interleukin-2-cultured, and Corynebacterium parvum (in vivo)-activated NK cells to induce RADCC. LGL-1 triggering of activated NK cells coincides with enhanced LGL-1 expression. Testing of murine mAbs to epitopes of CD2 only appears to augment RADCC induced by mAb NK-1.1 on fresh NK cells. Immunoprecipitation of the LGL-1 antigen reveals a highly disulfide-linked 40-kDa homodimer subunit that is N-glycosylated. Therefore, LGL-1 may be similar to other recently characterized NK-associated antigens such as NK-1.1, Ly-49, and NKR-PI. We conclude that although LGL-1 is expressed on "resting" NK cells, enhanced surface expression following activation is usually required for it to act as a signaling molecule.
Mason
L H
LH
Laboratory of Experimental Immunology, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201.
Yagita
H
H
Ortaldo
J R
JR
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D000920:Antibody-Dependent Cell Cytotoxicity / Q000502:physiology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology / Q000502:physiology*; D018801:CD2 Antigens; D002478:Cells, Cultured; D004357:Drug Synergism; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D007140:Immunoglobulin Fab Fragments; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000737:chemistry* / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D011233:Precipitin Tests; D017452:Receptors, IgG / Q000032:analysis / Q000276:immunology; D011971:Receptors, Immunologic / Q000276:immunology
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D000954:Antigens, Surface; D018801:CD2 Antigens; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D007376:Interleukin-2; D017452:Receptors, IgG; D011971:Receptors, Immunologic