Characterization of monoclonal antibodies to chitinase A1 and enhancement of chitinase A1 activity by monoclonal antibodies.
A total of eleven hybridomas which secrete antibodies against chitinase A1 were established. Among the eleven monoclonal antibodies (MAbs) obtained, six recognized the catalytic domain, three recognized the type III region and two recognized the chitin binding domain of chitinase A1. Of the two MAbs which recognized the chitin binding domain one was found to also react with chitinase D, but none of the other MAbs which recognized either the type III region or the chitin binding domain reacted with chitinase D despite the extensive amino acid sequence similarity between both the type III and chitin binding domains of the two chitinases. Two of the eleven MAbs enhanced chitinase activity significantly, while the other MAbs did not have any significant effect on chitinase A1 activity.
Sekine
S
S
Department of Biosystem Science, Graduate School of Science and Technology, Niigata University, Japan.
Ito
Y
Y
Hashimoto
M
M
Tanaka
H
H
Watanabe
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification / Q000494:pharmacology; D015153:Blotting, Western; D002688:Chitinases / Q000276:immunology / Q000378:metabolism*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D005260:Female; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000494:pharmacology; D007527:Isoenzymes / Q000276:immunology / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D016296:Mutagenesis; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D017384:Sequence Deletion
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D007527:Isoenzymes; D011994:Recombinant Proteins; D002688:Chitinases