title: Identification of a ToxR-activated gene, tagE, that lies within the accessory colonization factor gene cluster of Vibrio cholerae O395.
abstract: The nucleotide (nt) sequence has been determined for a Vibrio cholerae ToxR-activated gene designated tagE that is located within a cluster of genes required for efficient intestinal colonization. The tagE gene encompasses 909 nt and is predicted to encode a 303-amino-acid (aa) protein with an estimated molecular mass of 34,468 Da. Computer-assisted similarity searches revealed that TagE possesses aa sequence similarity with Escherichia coli OrfU and Staphylococcus simulans lysostaphin, two proteins that are involved in cell-wall biosynthesis and peptidoglycan degradation, respectively. The role, if any, that TagE plays in the accessory colonization factor phenotype is currently under investigation.
authors.affiliation: Department of Microbiology and Immunology, Louisiana State University Medical Center-Shreveport 71130-3932.
authors.forename: M E
authors.initials: ME
authors.lastname: Kovach
authors.forename: K J
authors.initials: KJ
authors.lastname: Hughes
authors.forename: K D
authors.initials: KD
authors.lastname: Everiss
authors.forename: K M
authors.initials: KM
authors.lastname: Peterson
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D036781:Fimbriae Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D018085:Regulon / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014734:Vibrio cholerae / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; C089973:TagE protein, Vibrio cholerae; D014157:Transcription Factors; C022924:colonization factor antigens; C499324:toxR protein, Vibrio cholerae; C066719:toxR protein, bacteria; D036781:Fimbriae Proteins