Tissue distribution of ecto-Mg-ATPase in adult and embryonic chicken.
We have determined the distribution of chicken ecto-Mg-ATPase in a variety of tissues from adult and embryonic chicken. The presence of ecto-Mg-ATPase was identified by an antibody raised against a 12 amino acid residue peptide, NH2-KILSGEEEGVFG, derived from proteolysis and sequencing of the chicken gizzard ecto-Mg-ATPase. Adult chicken tissues were also assayed for ATPase activity in the presence/absence of stimulators/inhibitors of the ecto-ATPase in order to confirm the immunologic tissue distribution results. There is controversy in the literature as to the size(s) of ecto-Mg-ATPases. We demonstrate here that the apparent size of the enzyme(s) recognized by anti-peptide antibodies by Western blot analysis depends on the denaturation conditions used prior to electrophoresis. Lastly, we deduce that the chicken ecto-ATPase is not identical to T-cadherin, as has been recently proposed.
Stout
J G
JG
Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Ohio 45267-0575.
Kirley
T L
TL
D000251:Adenosine Triphosphatases / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D002642:Chick Embryo; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis*; D005895:Gizzard, Avian / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008861:Microsomes / Q000201:enzymology*; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D009928:Organ Specificity; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology
D000906:Antibodies; D000939:Epitopes; D010446:Peptide Fragments; D000251:Adenosine Triphosphatases; C027201:ectoATPase