Blood levels of reduced/oxidized glutathione and plasma concentration of ascorbic acid during eccentric and concentric exercises of similar energy cost.
In an attempt to assess the possible oxidative stress associated with the transient exercise-induced activation of polymorphonuclear neutrophils (PMN), we compared the effects of eccentric and concentric exercises (downhill run: DR and uphill walk: UW, respectively) of equal duration (35 min) and similar energy cost (60% VO2max) on plasma levels of ascorbic acid ([AA]) and blood concentration of reduced ([GSH]) and oxidized ([GSSG]) glutathione. Eight healthy male subjects took part in this study. Plasma concentration of myeloperoxidase ([MPO]) was used as a specific marker of PMN activation. While there were no significant changes in [MPO] and [AA] in UW experiments, [MPO] increased (+80%) and [AA] decreased significantly during DR tests (P < 0.01 and P < 0.05, respectively). A significant negative relationship was observed between [AA] and [MPO] in DR experiments only (r = -0.49; P < 0.01). Mean (+/- SEM) basal GSH and GSSG concentrations, calculated by pooling the values measured before both tests, were 0.54 +/- 0.02 and 0.12 +/- 0.007 mM, respectively. The blood concentration of these compounds remained practically unchanged in both exercise tests. These results confirm the role played by the eccentric component of muscle contraction in transient exercise-induced PMN activation and suggest that this activation was partly involved in the decrease in [AA] observed in DR experiments. The oxidant stress associated with the exercise protocol used in this study was insufficient to alter blood levels of reduced and oxidized glutathione.
Camus
G
G
Laboratory of Human Applied Physiology, University of Liège. Belgium.
Felekidis
A
A
Pincemail
J
J
Deby-Dupont
G
G
Deby
C
C
Juchmes-Ferir
A
A
Lejeune
R
R
Lamy
M
M
D000328:Adult; D001205:Ascorbic Acid / Q000097:blood*; D004734:Energy Metabolism; D015444:Exercise / Q000502:physiology*; D005978:Glutathione / Q000097:blood*; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000502:physiology; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D009195:Peroxidase / Q000097:blood
D009195:Peroxidase; D005978:Glutathione; D001205:Ascorbic Acid