Stromal cell independent growth of bipotent B cell--macrophage precursors from murine fetal liver.
We have established stromal cell independent culture conditions under which single murine hematopoietic progenitor cells give rise to B lymphocytes and macrophages. IL-11, mast cell growth factor, and IL-7 are sufficient for the growth of B lineage cells from AA4.1+B220-Mac-1-Ly6A+ fetal liver cells isolated at day 12 of gestation. These conditions are also sufficient for commitment to myeloid differentiation, although CSF-1 is required for substantial growth of macrophages. The progenitors responding under these culture conditions: (i) require 8 days in culture before they become competent to respond to B cell mitogens in the presence of stromal cells and (ii) undergo a minimum of three cell divisions prior to Ig heavy chain allotype restriction. The findings suggest that the stromal cell dependency of immature hematopoietic cells may reflect a requirement for stromal cell derived growth factors. Further, this provides an in vitro system in which commitment and differentiation can be studied in single cells.
Kee
B L
BL
Wellesley Hospital Research Institute, Toronto, Ontario, Canada.
Cumano
A
A
Iscove
N N
NN
Paige
C J
CJ
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005314:Embryonic and Fetal Development / Q000502:physiology; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000502:physiology; D017370:Interleukin-11 / Q000494:pharmacology; D015851:Interleukin-7 / Q000494:pharmacology; D008099:Liver / Q000166:cytology* / Q000196:embryology*; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D019089:Stem Cell Factor; D017154:Stromal Cells / Q000502:physiology*
D016298:Hematopoietic Cell Growth Factors; D017370:Interleukin-11; D015851:Interleukin-7; D019089:Stem Cell Factor