title: Spontaneous production of granulocyte colony-stimulating factor in vitro by human B-lineage lymphocytes is a distinctive marker of germinal center cells.
abstract: The ability of human B lymphocytes to produce granulocyte (G)-CSF in vitro was investigated. Highly purified tonsillar B cells were fractionated into large and small cells by a Percoll density gradient, cultured, and tested for G-CSF gene expression. Large B cells spontaneous produced G-CSF mRNA and protein, whereas small B cells did not, even after incubation with various stimuli. Immunophenotypic analyses showed that large B lymphocytes contained approximately 60 to 70% of cells with the characteristic surface markers of germinal center (GC) B cells (CD38+, CD10+, and surface IgG+). The remaining cells expressed CD39, CD23, and surface IgD and were presumably in vivo-activated follicular mantle zone B cells. Fractionation of the large B lymphocytes into CD39+, surface IgD+, and CD39-, surface IgD- cells showed that the latter, but not the former, cell type produced G-CSF spontaneously in culture. Stimulation of purified (CD39-, surface IgD-) GC B cells with a CD40 mAb alone or in combination with IL-4 increased G-CSF production. Because these stimuli rescued a large fraction of GC cells (up to 50%) from spontaneous apoptosis in vitro, the finding may suggest that prevention of apoptotic death resulted in an increased G-CSF production or that CD40 mab and/or IL-4 increased G-CSF gene expression in G-CSF-producing GC B cells. Malignant B cells purified from the invaded lymph nodes of three patients with follicular center cell lymphoma and three Burkitt lymphoma cell lines, which had an immunophenotype identical with that of normal GC B cells, spontaneously produced G-CSF in vitro, thus confirming the GC origin of the cytokine. Incubation of normal purified GC B cells with rG-CSF resulted in the rescue of GC B cells from apoptosis, suggesting that G-CSF may be used by GC B cells in an autocrine manner. This autocrine loop of production and response to G-CSF by GC B cells may be activated by stimuli such as those delivered via the surface CD40 molecule, that participate in the rescue of GC B cells from apoptosis.
authors.affiliation: Laboratory of Oncology, Giannina Gaslini Scientific Institute, Genoa, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Corcione
authors.forename: L
authors.initials: L
authors.lastname: Baldi
authors.forename: S
authors.initials: S
authors.lastname: Zupo
authors.forename: M
authors.initials: M
authors.lastname: Dono
authors.forename: G B
authors.initials: GB
authors.lastname: Rinaldi
authors.forename: S
authors.initials: S
authors.lastname: Roncella
authors.forename: G
authors.initials: G
authors.lastname: Taborelli
authors.forename: M
authors.initials: M
authors.lastname: Truini
authors.forename: M
authors.initials: M
authors.lastname: Ferrarini
authors.forename: V
authors.initials: V
authors.lastname: Pistoia
mesh_terms: D000251:Adenosine Triphosphatases; D015703:Antigens, CD / Q000032:analysis; D017209:Apoptosis; D001081:Apyrase; D016175:B-Lymphocyte Subsets / Q000378:metabolism*; D015415:Biomarkers; D002478:Cells, Cultured; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D007072:Immunoglobulin D / Q000032:analysis; D066298:In Vitro Techniques; D014066:Palatine Tonsil / Q000166:cytology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D007072:Immunoglobulin D; D011947:Receptors, Antigen, B-Cell; D016179:Granulocyte Colony-Stimulating Factor; D000251:Adenosine Triphosphatases; D001081:Apyrase; C067869:CD39 antigen