Diversity in localisation of nitric oxide synthase antigen and NADPH-diaphorase histochemical staining in sacral somatic motor neurones of the cat.
Nitric oxide synthase (NOS) immunoreactivity occurred in about 60% of ventromedial, ventrolateral, and sphincteric motoneurones in cat sacral spinal cord. Proportions of sacral motoneurones histochemically stained for NADPH-diaphorase, were similar, and equivalent in size to those immunoreactive for NOS, suggesting co-localisation of diaphorase and NOS. Double staining techniques revealed that NOS co-localised with NADPH-diaphorase in approximately 60% of sacral motoneurones. Some remaining motoneurones exhibited neither NOS nor NADPH-diaphorase, while others exhibited only NOS or NADPH-diaphorase, indicating phenotypic differences among sacral motoneurones.
Pullen
A H
AH
Sobell Department of Neurophysiology, Institute of Neurology, London, UK.
Humphreys
P
P
D000818:Animals; D000941:Antigens / Q000276:immunology*; D002415:Cats; D007150:Immunohistochemistry; D009046:Motor Neurons / Q000276:immunology / Q000648:ultrastructure*; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D019001:Nitric Oxide Synthase / Q000276:immunology*; D013116:Spinal Cord / Q000276:immunology*
D000941:Antigens; D019001:Nitric Oxide Synthase; D009252:NADPH Dehydrogenase