Therapeutic effect of granulocyte colony-stimulating factor (G-CSF) on the protection against Listeria infection in SCID mice.
Anti-listerial activity in SCID mice as well as in control C.B-17 mice was augmented by granulocyte colony-stimulating factor (G-CSF). After 1 x 10(3) colony-forming units of Listeria monocytogenes (strain EGD) were intravenously inoculated, mice were intraperitoneally injected with G-CSF at a daily dose of 500 micrograms/kg for 5 days. The numbers of viable bacteria in the liver were significantly lower in G-CSF-treated SCID and C.B-17 mice than in non-treated mice. The surface marker analyses on gamma delta T-cell receptor (TcR), Mac-1 and F4/80, and dichlorofluorescein oxidative activity, showed a possible contribution of activated neutrophils, but not gamma/delta T cells nor activated macrophages, to the augmentation of anti-listerial activity in SCID mice. This study is one of the first reports on the anti-microbial effect of G-CSF in therapeutic use.
Kayashima
S
S
Department of Microbiology, National Defense Medical College, Saitama, Japan.
Tsuru
S
S
Hata
N
N
Rokutanda
M
M
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D007958:Leukocyte Count; D008089:Listeria monocytogenes / Q000302:isolation & purification; D008088:Listeriosis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D008099:Liver / Q000382:microbiology; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009504:Neutrophils / Q000276:immunology; D011994:Recombinant Proteins / Q000627:therapeutic use; D013154:Spleen / Q000473:pathology; D013950:Thymus Gland / Q000473:pathology
D000954:Antigens, Surface; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor