title: Staining pattern of type IV collagen and prognosis in early stage adenocarcinoma of the lung.
abstract: OBJECTIVE
  To examine the prognostic value of basement membrane expression in early stage adenocarcinoma of the lung.
  
  
  METHODS
  Using antibodies to type IV collagen, basement membrane expression at the tumour-stromal border was immunohistochemically analysed in 30 patients with early stage adenocarcinoma of the lung (pstage I and pstage II). Two patterns of staining for type IV collagen were observed: in the first one the staining line was conserved or partially fragmented; in the second the staining line was widely fragmented or absent in more than 10% of the tumour area. The first staining pattern was categorised as continuous and the second as discontinuous.
  
  
  RESULTS
  Of the 24 patients with pstage I adenocarcinoma, 12 (50%) cases showed a continuous pattern. In only one (16.7%) of the six patients with pstage II adenocarcinoma was this pattern evident. Five year survival was greater in pstage I adenocarcinoma (65%) than in pstage II adenocarcinoma (17%), but the difference was not significant. When the analysis was restricted to the 24 patients with pstage I adenocarcinoma, five year survival was better in continuous pattern cases (88%) than in discontinuous pattern cases (20.5%) (p < 0.05). The survival curve of 12 patients with pstage I adenocarcinoma and a discontinuous pattern resembled that of the six patients with pstage II adenocarcinoma.
  
  
  CONCLUSIONS
  These findings suggest that patients with pstage I adenocarcinoma and a discontinuous pattern have histopathologically unrecognised micrometastasis when they come to surgery. The staining pattern of type IV collagen could help in the prognosis of pstage I adenocarcinoma of the lung after surgery.
authors.affiliation: First Department of Medicine, School of Medicine, Hokkaido University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: I
authors.initials: I
authors.lastname: Nakajima
authors.forename: S
authors.initials: S
authors.lastname: Abe
authors.forename: S
authors.initials: S
authors.lastname: Ogura
authors.forename: H
authors.initials: H
authors.lastname: Isobe
authors.forename: Y
authors.initials: Y
authors.lastname: Kawakami
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000473:pathology; D001485:Basement Membrane / Q000473:pathology; D003094:Collagen / Q000032:analysis*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D013194:Staining and Labeling
chemical_list: D003094:Collagen