Phagocytosis of intravenously administered particles by leukocytes adhered to the aortic endothelium of the rat.
Phagocytosis has been used to characterize on a functional basis leukocytes adhered to the aortic endothelium of the rat. After intravenous administration of particles, phagocytosis was observed microscopically in esterase-positive leukocytes adhered to the endothelium in whole mounts of aorta. Polybead blue and red, 0.5 and 1 microns particle size, were inadequate because they were insufficiently colored to be identified individually at 400 x. Fluoresbrite YG 0.25 and 0.50 micron at doses of 0.2 and 2 x 0.3 ml/100 g, respectively, produced endothelial lesions. The same occurred with Monastral blue B (MbB) at 0.3 ml/100 g, red iron at 2 x 16 mg/100 g and India ink at different concentrations depending on the supplier. At lower particle doses, lesions were not found. Deferoxamine mesylate 1.5 mg/100 g intravenous and allopurinol 5 mg/100 g intraperitoneal administered before the particles diminished the number and intensity of lesions. In none of the cases studied was the percentage of phagocytic cells greater than 50%. Clearance curves of MbB and Fluoresbrite indicated rapid disappearance of particles from the blood. Results indicate that administration of particulate suspensions is not a good method for characterizing the phagocytic leukocytes adhering to the aortic endothelium because low doses produce rapid clearance of particles, thus impeding sufficient leukocyte loading, and higher doses produce endothelial lesions that often impair reliable counting of the adhering leukocytes.
Gabaldón
M
M
Research Center, Hospital La Fe, Valencia, Spain.
Capdevila
C
C
Zúñiga
A
A
D000818:Animals; D002244:Carbon; D002448:Cell Adhesion; D004396:Coloring Agents; D003676:Deferoxamine; D004730:Endothelium, Vascular / Q000166:cytology*; D005290:Ferric Compounds; D007211:Indoles; D007275:Injections, Intravenous; D007962:Leukocytes / Q000502:physiology*; D008163:Luminescent Measurements; D008297:Male; D008863:Microspheres; D009942:Organometallic Compounds; D010316:Particle Size; D010587:Phagocytosis; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling
D004396:Coloring Agents; D005290:Ferric Compounds; D007211:Indoles; D009942:Organometallic Compounds; C028433:chinese ink; C000499:ferric oxide; C015445:copper phthalocyanine; D002244:Carbon; D003676:Deferoxamine