A simple method for demonstration of eosinophils in frozen sections.
Tissue eosinophils can be easily and specifically demonstrated in frozen sections after substrateless incubation with 3,3'-diaminobenzidine (DAB) at neutral pH. Under such conditions, other cells possessing endogenous peroxidatic activity (neutrophils, macrophages) do not stain, whereas staining of mitochondria-rich cells due to cytochrome oxidase activity can be avoided by a short prefixation of sections in glutaraldehyde-containing fixatives. The method can be useful in cases when a rapid screening of tissue eosinophils is required.
Furgał
A
A
Department of Histology, Jagiellonian University School of Medicine, Kraków, Poland.
Litwin
J A
JA
D015100:3,3'-Diaminobenzidine; D000818:Animals; D003238:Connective Tissue / Q000648:ultrastructure; D003239:Connective Tissue Cells; D003576:Electron Transport Complex IV / Q000276:immunology / Q000378:metabolism; D004804:Eosinophils / Q000201:enzymology / Q000648:ultrastructure*; D005615:Freezing; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D010544:Peroxidases / Q000032:analysis / Q000378:metabolism; D051381:Rats; D013194:Staining and Labeling; D016707:Tissue Fixation
D015100:3,3'-Diaminobenzidine; D010544:Peroxidases; D003576:Electron Transport Complex IV