A vector driving the expression of foreign cDNAs in the MHC class II-positive cells of transgenic mice.
We describe a plasmid vector that drives the expression of foreign cDNAs in transgenic mice, according to the dictates of an MHC class II gene promoter. Using this vector, we have often obtained mRNA and protein synthesis with a tissue and cell-type specificity indistinguishable from that of the endogenous MHC class II genes.
Kouskoff
V
V
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Institut de Chimie Biologique, Faculté de Médecine, Strasbourg, France.
Fehling
H J
HJ
Lemeur
M
M
Benoist
C
C
Mathis
D
D
D000818:Animals; D000917:Antibody Formation / Q000276:immunology; D018076:DNA, Complementary / Q000235:genetics*; D000939:Epitopes / Q000276:immunology; D015870:Gene Expression / Q000235:genetics*; D005802:Genes, MHC Class II / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics*; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011817:Rabbits
D018076:DNA, Complementary; D000939:Epitopes; D000949:Histocompatibility Antigens Class II