Platelet derived wound healing factors (PDWHF) accelerate and augment wound healing angiogenesis in the rat.
The object of this study was to determine the effect of PDWHF on wound healing angiogenesis. Sponges saturated with PDWHF were implanted in rats. Six hours to 14 days post-implantation, vascular corrosion casts were prepared and examined by SEM. Leukocyte margination and angiogenesis was accelerated by 24-48 hours. Vessel number was also greater than in the controls. Another series of PDWHF treated sponges were implanted. Fourteen days later the vasculature was perfused with Evans blue. Sponges were homogenized, and absorbance of the supernatants was determined. Absorbances of PDWHF supernatants was 2X greater than controls. PDWHF enhanced wound healing angiogenesis in the rat.
Phillips
G D
GD
Department of Surgery, University of Minnesota, Minneapolis 55455.
Stone
A M
AM
Whitehead
R A
RA
Knighton
D R
DR
D000818:Animals; D001792:Blood Platelets; D045424:Complex Mixtures; D016319:Corrosion Casting; D005070:Evans Blue; D006133:Growth Substances / Q000494:pharmacology*; D008297:Male; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D010477:Perfusion; D051381:Rats; D017207:Rats, Sprague-Dawley; D015503:Surgical Sponges; D014945:Wound Healing / Q000187:drug effects*
C078881:CT-102 activated platelet supernatant; D045424:Complex Mixtures; D006133:Growth Substances; D005070:Evans Blue