Micronucleated erythrocytes as an assay to assess actions by physical and chemical genotoxic agents in Clarias gariepinus.
An in vivo study on the effects of both physical (gamma-radiation) and chemical (mitomycin C) genotoxic agents was carried out with the catfish species, Clarias gariepinus. The fish were either exposed to gamma-radiation at doses of 0-9 Gy or injected intraperitoneally with mitomycin C at concentrations of 0-2 mg/kg. Micronucleated erythrocytes were sampled from 0 to 60 days post treatment. Data obtained showed a time-dependent response of the induction of micronucleated erythrocytes with both genotoxic agents. A linear dose-dependent increase was observed 2-4 days after treatment. These data show the importance of sampling time in the micronucleus assay with Clarias gariepinus.
Bahari
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IB
Department of Nuclear Science, Faculty of Physical and Applied Sciences, Universiti Kebangsaan Malaysia, Bangi, Selangor D.E.
Noor
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FM
Daud
N M
NM
D000818:Animals; D002397:Catfishes; D004912:Erythrocytes / Q000187:drug effects* / Q000528:radiation effects*; D005720:Gamma Rays; D015162:Micronucleus Tests; D016685:Mitomycin / Q000633:toxicity*; D013997:Time Factors
D016685:Mitomycin