Physical mapping of the human hprt chromosomal region (Xq26).
The human hprt chromosomal region (Xq26) was physical-mapped using pulsed field gel electrophoresis (PFGE). This work involved: (i) the recovery of three new genomic DNA markers (DXS1327, DXS1328, and DXS1329), (ii) the ordering of new markers relative to 11 previously available hprt-linked markers by deletion mapping, and (iii) the completion of human T-lymphocyte PFGE Southern blots using the 14 Xq26 markers. A contiguous 1.5-Mb physical map of the region telomeric to hprt was determined. As this map identifies clusters of in vivo unmethylated rare-cutter restriction sites, potential CpG islands are revealed.
Lippert
M J
MJ
University of Vermont, VCC Genetics Laboratory, Burlington 05401.
Albertini
R J
RJ
Nicklas
J A
JA
D000328:Adult; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D008214:Lymphocytes; D008297:Male; D014960:X Chromosome
D015342:DNA Probes; D007041:Hypoxanthine Phosphoribosyltransferase; D004262:DNA Restriction Enzymes