Modulation of cytokine expression by cAMP analogs in myc-immortalized microglial cell lines.
Expression of cytokines can be modulated by cAMP in macrophages or in primary microglial cultures. Similar to what is observed in normal conditions, treatment of immortal microglial cell lines with dibutyryl-cAMP blocked the accumulation of TNF alpha transcripts induced by lipopolysaccharide, whereas activation of Interleukin-1 alpha (IL-1 alpha) remained unaffected. Immortalized cell lines can therefore be regarded as a valid model to test the immune responsiveness of microglial cells in the presence of neuro-endocrine agents modulating cAMP levels.
Righi
M
M
CNR, Center of Cytopharmacology, School of Medicine, University of Milan, Italy.
D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects / Q000276:immunology; D000242:Cyclic AMP / Q000494:pharmacology*; D016207:Cytokines / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D003720:Densitometry; D015870:Gene Expression; D016259:Genes, myc / Q000187:drug effects* / Q000276:immunology*; D007116:Immunization, Passive; D007378:Interleukins / Q000378:metabolism; D008070:Lipopolysaccharides / Q000378:metabolism; D051379:Mice; D017628:Microglia / Q000187:drug effects / Q000276:immunology*; D012313:RNA / Q000187:drug effects / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D007378:Interleukins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D012313:RNA; D000242:Cyclic AMP