Involvement of the brain type of ryanodine receptor in T-cell proliferation.
Cloning and sequence analysis of cDNA showed that the brain type of ryanodine receptor (RYR) is expressed in human Jurkat T-lymphocyte cells. Fura-2 measurements revealed that the RYR in T-cells functions as a ryanodine-sensitive, caffeine-insensitive Ca2+ release channel. Furthermore, ryanodine stimulated proliferation and altered the growth pattern of cultured human T-cells when added together with FK506.
Hakamata
Y
Y
Department of Geriatric Medicine, Kyoto University Faculty of Medicine, Japan.
Nishimura
S
S
Nakai
J
J
Nakashima
Y
Y
Kita
T
T
Imoto
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D001923:Brain Chemistry; D002110:Caffeine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D012433:Ryanodine / Q000494:pharmacology; D019837:Ryanodine Receptor Calcium Release Channel; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000166:cytology* / Q000187:drug effects; D016559:Tacrolimus / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D015220:Calcium Channels; D018076:DNA, Complementary; D009124:Muscle Proteins; D012333:RNA, Messenger; D019837:Ryanodine Receptor Calcium Release Channel; D012433:Ryanodine; D002110:Caffeine; D002118:Calcium; D016559:Tacrolimus