title: Evaluation of indeterminate sera for hepatitis C virus by assays using synthetic peptides.
abstract: The development of new immunodiagnostic systems to study antibodies anti-HCV based on the use of synthetic peptides are potentially very important in the evaluation of indeterminate samples. We have compared two immunodiagnostic tests, the 3-RIBA and LIA-HCV test system, to examine the samples rated as indeterminate by the 2-RIBA test. The results showed that the specificity of the new tests has improved, in fact 29/40 of the indeterminate samples studied became positive with both tests. However, the 3-RIBA test showed higher sensitivity, since two samples negative by LIA-HCV, were positive with this system.
authors.affiliation: Istituto di Microbiologia, Facoltà di Medicina e Chirurgia La Sapienza, Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Rivanera
authors.forename: G
authors.initials: G
authors.lastname: Lorino
authors.forename: D
authors.initials: D
authors.lastname: Lilli
authors.forename: G
authors.initials: G
authors.lastname: Dicuonzo
authors.forename: C
authors.initials: C
authors.lastname: Mancini
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000097:blood*; D006526:Hepatitis C / Q000097:blood* / Q000453:epidemiology; D018937:Hepatitis C Antibodies; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D010455:Peptides; D012680:Sensitivity and Specificity
chemical_list: D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D010455:Peptides