[Antigen mimicry of anti-idiotypic antibodies for human ovarian carcinoma antigen: generation and characterization of anti anti-idiotype antibodies].
The antigen mimicry of monoclonal anti-idiotypic antibodies 6B11 and 1H12 was investigated, which carrying the internal image of human ovarian carcinoma antigen. Anti-anti-idiotypic antibodies, obtained from the mice immunized with 6B11 or 1H12, binded to ovarian tumor antigen OC 166-9 specifically by enzyme linked immunosorbent assay (ELISA). Western blot analysis showed that Ab3 and Ab1 recognized the same antigenic protein. Through antibody dependent cell-mediated cytotoxicity (ADCC), Ab3 sera killed the ovarian carcinoma cell line SKOV3 specifically in vitro. These results suggest that 6B11 and 1H12 may substitute for the nominal antigen to stimulate a specific immune response and that they are potential candidates as idiotype vaccines against ovarian carcinomas.
Lu
W Y
WY
Research Laboratory of Gynecologic Oncology, People's Hospital, Beijing Medical University.
Qian
H N
HN
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D000939:Epitopes