Detection of P glycoprotein activity on normal and leukemic CD34+ cells.
Rhodamine 123 is transported by the transmembrane efflux pump P glycoprotein (Pgp). We used this fluorescent dye to study multidrug resistance (MDR) activity in normal and leukemic CD34+ cells. These immature cells had a high degree of MDR activity. Among leukemic cells, CD34+ leukemias had significantly higher MDR activity as compared to CD34- leukemias. Heterogeneous results in cell subpopulations, however, indicate that prognosis should be interpreted in the light of MDR analysis.
Drenou
B
B
Laboratoire d'Hématologie-Immunologie, Centre Hospitalier Régional et Universitaire, Rennes, France.
Fardel
O
O
Amiot
L
L
Fauchet
R
R
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000208:Acute Disease; D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D001853:Bone Marrow / Q000473:pathology*; D001854:Bone Marrow Cells; D002352:Carrier Proteins / Q000032:analysis*; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D005500:Follow-Up Studies; D006801:Humans; D016130:Immunophenotyping; D007938:Leukemia / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000032:analysis*; D009000:Monocytes / Q000166:cytology / Q000473:pathology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000145:classification / Q000473:pathology; D012016:Reference Values; D020112:Rhodamine 123; D012235:Rhodamines; D013997:Time Factors
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015703:Antigens, CD; D018952:Antigens, CD34; D002352:Carrier Proteins; D005456:Fluorescent Dyes; D008562:Membrane Glycoproteins; D012235:Rhodamines; D020112:Rhodamine 123