Metal-induced genotoxic adaptation in barley (Hordeum vulgare L.) to maleic hydrazide and methyl mercuric chloride.
Presoaked seeds of barley, Hordeum vulgare L., were exposed for 2 h to maleic hydrazide (MH), 5 x 10(-2) M or methyl mercuric chloride (MMCl), 10(-4) M with or without a prior conditioning with MH, 5 x 10(-3) M; MMCl, 10(-5) M; cadmium sulfate (CdSO4), 10(-4) M or zinc sulfate (ZnSO4), 10(-1) M; the interexposure time was 2 h. Subsequently as the seeds germinated a number of endpoints were measured that included mitotic index, mitotic chromosome aberrations and micronuclei (MNC) in embryonic shoot cells fixed at 32, 36, 40, 44, 48 and 52 h of recovery, and seedling height on day 7. The results demonstrated that prior conditioning exposure to MH or metals induced genotoxic adaptation to the subsequent challenge exposure to MH and MMCl. Cadmium-induced genotoxic adaptation against either MH or MMCl challenge exposure was, however, significantly prevented when the presoaked seeds were pre-exposed to buthionine sulfoximine, 10(-3) M for 2 h, thereby providing evidence that the underlying mechanism of genotoxic adaptation possibly involved phytochelatins.
Subhadra
A V
AV
Department of Botany, Berhampur University, Orissa, India.
Panda
B B
BB
D000222:Adaptation, Physiological; D016587:Antimutagenic Agents / Q000378:metabolism*; D019328:Buthionine Sulfoximine; D002104:Cadmium / Q000378:metabolism / Q000494:pharmacology; D019187:Cadmium Compounds; D002869:Chromosome Aberrations; D004260:DNA Repair; D005978:Glutathione; D001467:Hordeum / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D008300:Maleic Hydrazide / Q000378:metabolism / Q000633:toxicity; D008628:Mercury / Q000494:pharmacology; D008667:Metalloproteins / Q000378:metabolism*; D008670:Metals / Q000494:pharmacology*; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives / Q000378:metabolism; D008767:Methylmercury Compounds / Q000378:metabolism / Q000633:toxicity; D008940:Mitotic Index; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity*; D054811:Phytochelatins; D010940:Plant Proteins / Q000378:metabolism*; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D008941:Spindle Apparatus / Q000187:drug effects; D013431:Sulfates / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology; D017967:Zinc Compounds / Q000378:metabolism; D019287:Zinc Sulfate
D016587:Antimutagenic Agents; D019187:Cadmium Compounds; D008667:Metalloproteins; D008670:Metals; D008767:Methylmercury Compounds; D009153:Mutagens; D010940:Plant Proteins; D013431:Sulfates; D017967:Zinc Compounds; D002104:Cadmium; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; D019287:Zinc Sulfate; C037123:cadmium sulfate; D054811:Phytochelatins; D008628:Mercury; D005978:Glutathione; D015032:Zinc; D008300:Maleic Hydrazide