The potentiation of in vitro antibody response in low-responding mice by addition of cytokines.
The effect of exogenous cytokines on the antibody response to ARS-BGG after in vitro immunization in high- and low-responding strains of mice was determined. Twenty units/ml was established to be the optimal dose of cytokines. The low IgG antibody response to ARS-BGG in low-responding B10 mice was increased significantly by addition of either IL-4 or IFN-gamma. Similarly, the lower production of IgA antibodies was elevated by addition of either IL-2, IL-4, IL-5 or IFN-gamma. The levels of IgM were not influenced by any of the cytokines tested. Cytokines, therefore, play a fundamental role in regulating the responsiveness to protein antigen.
Vĕtvicka
V
V
Department of Microbiology and Immunology, School of Medicine, University of Louisville, KY 40292.
Vĕtvicková
J
J
Sonnenfeld
G
G
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects* / Q000276:immunology; D001147:Arsanilic Acid / Q000276:immunology*; D001152:Arsenicals; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006241:Haptens / Q000276:immunology*; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology; D005719:gamma-Globulins / Q000276:immunology*
D001152:Arsenicals; D006241:Haptens; D008222:Lymphokines; C088471:arsanilic acid hapten-bovine gamma globulin; D005719:gamma-Globulins; D001147:Arsanilic Acid