Chromosomal aberrations in mouse lymphocytes exposed in vitro and in vivo to benzidine and 5 related aromatic amines.
Mouse lymphocytes were exposed in vitro for 2 h or in vivo for 24 h to benzidine and related aromatic amines to test for chromosome aberrations (CA) and mitotic indices. Uninduced mouse S9 was used to activate the amines for the in vitro tests to be consistent with the in vivo tests. Contrary to a previous report, no difference could be established in the genotoxicity of benzidine following activation with uninduced S9 compared to induced S9. There were concentration related increases in CA for benzidine and all the amines in vitro except for 4,4'-diaminostilbene which exhibited the greatest cellular toxicity towards cultured lymphocytes. Benzidine and its derivatives showed significant increases in CA in vivo compared to its negative control. The CA values for 4-aminostilbene were significantly higher than the other amines in both in vivo and in vitro studies. These genotoxicity results for 4-aminostilbene are consistent with our previous report of the pronounced CA effects in murine bone-marrow cells but would not be predicted from Salmonella mutagenicity tests.
Das
L
L
Department of Human Genetics, Medical School, University of Michigan, Ann Arbor 48109-1065.
Das
S K
SK
Hooberman
B H
BH
Chu
E H
EH
Sinsheimer
J E
JE
D000611:Aminobiphenyl Compounds / Q000633:toxicity*; D000818:Animals; D001560:Benzidines / Q000633:toxicity*; D002869:Chromosome Aberrations; D008110:Liver Extracts; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008862:Microsomes, Liver / Q000201:enzymology; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D013267:Stilbenes / Q000633:toxicity*; D013329:Structure-Activity Relationship
D000611:Aminobiphenyl Compounds; D001560:Benzidines; D008110:Liver Extracts; D009153:Mutagens; D013267:Stilbenes; C016228:4-aminostilbene