Rapid method for isolating detergent-insoluble outer membrane proteins from Pseudomonas aeruginosa.
The present study compares two techniques for isolating outer membrane proteins (OMPs) of Pseudomonas aeruginosa, Method A - selective solubilization with sodium lauryl sarcosinate, and Method B - isopycnic sucrose gradient centrifugation, using three criteria: the amount of proteins obtained, polypeptide patterns after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their practical aspects. Method A appears to be superior to Method B. It yields a higher outer membrane protein content and a similar polypeptide pattern as Method B, but is more rapid and less cumbersome.
Ravaoarinoro
M
M
Department of Microbiology and Infectious Diseases, Hôtel-Dieu de Montréal, Québec, Canada.
Ciurli
C
C
Toma
E
E
Morisset
R
R
D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D002500:Centrifugation, Isopycnic; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D010455:Peptides / Q000032:analysis*; D011550:Pseudomonas aeruginosa; D015203:Reproducibility of Results; D012521:Sarcosine / Q000031:analogs & derivatives; D012995:Solubility; D013997:Time Factors
D001425:Bacterial Outer Membrane Proteins; D003902:Detergents; D010455:Peptides; C025231:sarkosyl; D012521:Sarcosine