Identification of differentially expressed members of tobacco homeobox families by differential PCR.
As a first step to investigate the functions of homeobox genes in tobacco genetic tumorigenesis, we have used polymerase chain reaction to identify Hot (Homeobox in tobacco) genes that are expressed in tobacco genetic tumors. Five Hot genes that are actively expressed in tobacco genetic tumors are identified. Particularly, Hot1 is profoundly abundant in tumorous tissues, suggesting that it acts as a positive regulator of cell growth and differentiation during genetic tumorigenesis.
Feng
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Center for Agricultural Biotechnology, Maryland Biotechnology Institute, College Park.
Kung
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D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D017124:Conserved Sequence; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D017931:DNA Primers; D015870:Gene Expression; D005801:Genes, Homeobox; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010941:Plant Tumors; D010947:Plants, Toxic; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism*
D017931:DNA Primers; D012313:RNA; D004247:DNA