An effective method for depleting mature T lymphocytes from bone marrow cells--two-step Percoll centrifugation.
In the present paper we have observed the effect of discontinuous gradient two-step Percoll centrifugation on depleting mature T lymphocytes from normal bone marrow. The pre-/post-Percoll percentage of CD34+, and Leu4+ cells in MNC was counted by using APAAP technique. As the two-step Percoll centrifugation is simple, and time saving, and decreases the incidence of contamination of cultured cells as well, This technique may be of value in serum-free culture of hematopoietic cells and immunological studies.
Tang
J S
JS
Institute of Hematology, Xiehe Hospital, Tongji Medical University, Wuhan.
Wang
B M
BM
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D000943:Antigens, Differentiation / Q000032:analysis; D001854:Bone Marrow Cells; D002498:Centrifugation / Q000379:methods; D006801:Humans; D013601:T-Lymphocytes
D015703:Antigens, CD; D018952:Antigens, CD34; D000943:Antigens, Differentiation