Localization of nitric oxide synthase immunoreactivity in mast cells of human nasal mucosa.
Nitric oxide (NO)-synthase immunoreactivity has been detected for the first time in mast cells of human normal nasal mucosa, with an antibody specific for neuronal NO-synthase. Intense immunoreactivity was revealed in secretion granules of mast cells but was found in mast cell granules free in the extracellular matrix only in some instances; no reactivity was found in the cytoplasm of this or other cell types. These findings suggest that human nasal mast cells contain a particulate isoform of NO-synthase, which shares epitopes with neuronal NO-synthase and is rapidly removed from granules upon exocytosis.
Bacci
S
S
Department of Human Anatomy and Histology, Section E. Allara, University of Florence, Italy.
Arbi-Riccardi
R
R
Mayer
B
B
Rumio
C
C
Borghi-Cirri
M B
MB
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000201:enzymology*; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009297:Nasal Mucosa / Q000201:enzymology*; D019001:Nitric Oxide Synthase
D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases