Immunolocalisation of chromogranin B, secretogranin II, calcitonin gene-related peptide and substance P at developing and adult neuromuscular synapses.
The localisation of chromogranin B, secretogranin II, calcitonin gene-related peptide and substance P was characterised by the use of indirect immunofluorescence techniques at neuromuscular junctions in frog, mouse and rat muscles. We found that chromogranin B, secretogranin II and calcitonin gene-related peptide are co-expressed in the frog, 1 week old mouse and 1 week old rat endplates. Substance P immunoreactivity could only be detected in frog muscles.
Andreose
J S
JS
C.N.R. Center of Cytopharmacology, Department of Medical Pharmacology, University of Milano, Italy.
Sala
C
C
Fumagalli
G
G
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D053378:Chromogranin B; D002864:Chromogranins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D051379:Mice; D009469:Neuromuscular Junction / Q000378:metabolism* / Q000648:ultrastructure; D009479:Neuropeptides / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D011894:Rana pipiens; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism; D013373:Substance P / Q000378:metabolism
D053378:Chromogranin B; D002864:Chromogranins; D009479:Neuropeptides; D011506:Proteins; D011950:Receptors, Cholinergic; C506137:chromogranin B, mouse; C506140:chromogranin B, rat; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide