Conjugation of synthetic peptides to carrier iscoms: factors affecting the immunogenicity of the conjugate.
This study was designed to explore optimal conditions for the conjugation of a synthetic peptide to preformed influenza virus iscoms using MHS (maleimidohexanoyl-N-hydroxy-succinimide ester) as coupling agent. The peptide used in this study comprised amino acids 122-138 of porcine growth hormone (pGH). Different ratios of peptide to carrier iscoms were tested and the resulting conjugates were analysed for composition, antigenicity and immunogenicity. The problem of low solubility and poor immunogenicity of high-density peptide conjugates is discussed and a general protocol for conjugation of peptides to carrier iscoms is proposed.
Lövgren
K
K
Swedish University of Agricultural Sciences, College of Veterinary Medicine, Department of Veterinary Microbiology, Uppsala.
Larsson
M
M
D000818:Animals; D000917:Antibody Formation; D002352:Carrier Proteins / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D005260:Female; D013006:Growth Hormone / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D016882:ISCOMs / Q000737:chemistry / Q000276:immunology*; D007114:Immunization; D007117:Immunization, Secondary; D007252:Influenza Vaccines / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D013552:Swine; D018074:Vaccines, Conjugate / Q000737:chemistry / Q000276:immunology
D002352:Carrier Proteins; D000939:Epitopes; D016882:ISCOMs; D007252:Influenza Vaccines; D010446:Peptide Fragments; D018074:Vaccines, Conjugate; D013006:Growth Hormone