Long-range mapping of the 11q23 region involved in chromosome aberrations in human tumors by pulsed-field gel electrophoresis with a yeast artificial chromosome.
We have previously demonstrated that the RCK gene involved in t(11;14)(q23;q32) and the more centromeric MLL/ALL1 gene involved in t(4;11)(q21;q23) and t(11;19)(q23;p13) are localized on different adjacent NotI fragments by using pulsed-field gel electrophoresis (PFGE) analysis with the yeast artificial chromosome (YAC) clone yB22B2. The PFGE analysis using the YACs of YTY17 containing the prophobilinogen deaminase (PBGD), CBL2 and THY1 genes and yB22B2 allowed the following ordering of genes and breakpoints from CD3 to THY1 on 11q23: cent-CD3-ALL/MLL1-RCK-PBGD-CBL2-THY1, and the establishment of a long-range restriction map covering these genes. In addition, we showed that the FLI1 region involved in the t(11;22)(q24;q12) in Ewing's sarcoma was more telomeric region that the THY1 gene by analyzing somatic cell hybrids carrying the 11q- and/or 14q+ chromosome of the t(11;14)(q23;q32) translocation, and by PFGE analysis of the YAC clone YTY17.
Akao
Y
Y
Department of Anatomy and Biology, Osaka Medical College, Takatsuki, Japan.
Tsujimoto
Y
Y
Seto
M
M
Imai
T
T
Bergeron
D
D
Berbeau
B
B
Otsuki
Y
Y
D000208:Acute Disease; D000954:Antigens, Surface / Q000235:genetics; D015139:Blotting, Southern; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002880:Chromosomes, Human, Pair 11; D002883:Chromosomes, Human, Pair 14; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D006822:Hybrid Cells; D011163:Hydroxymethylbilane Synthase / Q000235:genetics; D007938:Leukemia / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000235:genetics; D005810:Multigene Family / Q000235:genetics; D050722:Oncogene Protein v-cbl; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics; D018800:Thy-1 Antigens; D014178:Translocation, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D015342:DNA Probes; D004273:DNA, Neoplasm; D008562:Membrane Glycoproteins; D050722:Oncogene Protein v-cbl; D015682:Retroviridae Proteins, Oncogenic; D018800:Thy-1 Antigens; D011163:Hydroxymethylbilane Synthase