Identification of the Staphylococcus sciuri species group with EcoRI fragments containing rRNA sequences and description of Staphylococcus vitulus sp. nov.
Strains of a new species, Staphylococcus vitulus, were isolated from food and a variety of mammals. This species was recognized on the basis of the results of an analysis of genomic EcoRI fragments containing portions of the rRNA operons. The patterns of hybridized fragments obtained from strains belonging to the new taxon were sorted into a distinguishable cluster and were distinct from the Staphylococcus lentus and Staphylococcus sciuri patterns. The results of DNA-DNA hybridization reactions demonstrated that strains in this cluster were more closely related to S. lentus and S. sciuri than to other Staphylococcus species and yet were significantly different. While these strains had some of the phenotypic characteristics of the S. sciuri species group, the newly recognized taxon could be distinguished by its very small colonies on P agar, absence of alkaline phosphatase activity, and lack of acid production from L-arabinose, maltose, N-acetylglucosamine, D-mannose, and raffinose. The type strain of the new species is strain DD 756 (= ATCC 51145).
Webster
J A
JA
E. I. du Pont de Nemours and Company, Wilmington, Delaware 19880-0402.
Bannerman
T L
TL
Hubner
R J
RJ
Ballard
D N
DN
Cole
E M
EM
Bruce
J L
JL
Fiedler
F
F
Schubert
K
K
Kloos
W E
WE
D000818:Animals; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D015246:Deoxyribonuclease EcoRI; D005516:Food Microbiology; D006801:Humans; D008322:Mammals / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D009876:Operon; D010457:Peptidoglycan / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D013045:Species Specificity; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D013682:Teichoic Acids / Q000032:analysis
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D010457:Peptidoglycan; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D013682:Teichoic Acids; D015246:Deoxyribonuclease EcoRI