Differential RNA editing in closely related introns in Oenothera mitochondria.
Introns a/b of the nad2 gene and b/c of the nad1 gene in Oenothera mitochondria were found to be closely related. Within a scaffold of conserved sequence regions, a 48 bp sequence element covering intron domain V and flanking nucleotides is identical in both group II introns. The third nucleotide of this element is edited in the nad2, but not in the nad1 intervening sequence. The C to U editing event compensates an nad2-specific nucleotide mismatch in the stem domain IV and thus improves secondary structure stability. This differential editing event indicates that the identical upstream 2 and downstream 45 nucleotides are not sufficient to specify this editing site. Comparison of adjacent exon editing patterns in spliced and unspliced transcripts shows a higher degree of editing in processed sequences, confirming that RNA editing is a posttranscriptional process in plant mitochondria.
Lippok
B
B
Institut für Genbiologische Forschung, Berlin, Germany.
Brennicke
A
A
Wissinger
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D005075:Biological Evolution; D017124:Conserved Sequence; D005091:Exons; D007438:Introns; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010940:Plant Proteins / Q000235:genetics; D010944:Plants / Q000235:genetics*; D012313:RNA / Q000378:metabolism*; D017393:RNA Editing; D000077278:RNA, Mitochondrial; D015183:Restriction Mapping
D010940:Plant Proteins; D000077278:RNA, Mitochondrial; D012313:RNA