Pulsatile release of gonadotrophin releasing hormone (GnRH) is an intrinsic property of GT1 GnRH neuronal cell lines.
The GT1-1 gonadotrophin releasing hormone (GnRH) cell line releases GnRH in a pulsatile fashion. This finding demonstrates that the generation and synchronization of GnRH pulses is an intrinsic property of GnRH neurons. It appears that propagated action potentials and a Ca(2+)-coupled secretory mechanism are necessary for pulsatile release. The secretion of GnRH from GT1 cells is stimulated by both norepinephrine and dopamine respectively via beta 1-adrenergic and D1-dopaminergic receptors. These findings provide new data for the understanding of the complex neuroendocrine regulation of luteinizing hormone.
Weiner
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Reproductive Endocrinology Center, University of California, San Francisco 94143.
Martinez de la Escalera
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D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000200:Action Potentials; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D010507:Periodicity; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000502:physiology; D017447:Receptors, Dopamine D1 / Q000187:drug effects / Q000502:physiology; D026941:Sodium Channel Blockers; D015222:Sodium Channels / Q000502:physiology; D014407:Tumor Cells, Cultured
D011943:Receptors, Adrenergic, beta; D017447:Receptors, Dopamine D1; D026941:Sodium Channel Blockers; D015222:Sodium Channels; D007987:Gonadotropin-Releasing Hormone; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine; D004298:Dopamine; D009638:Norepinephrine