title: Heat-shock mannoproteins as targets of secretory IgA in Candida albicans.
abstract: The expression of Candida albicans antigenic determinants reacting with secretory IgA from patients with oral and vaginal candidiasis was investigated under different in vitro conditions. Reversible antigenic transitions were inducible in synthetic medium by temperature shifts, as the yeast cells were positive by an indirect immunofluorescence assay after being incubated at 37 degrees C but not at 25 degrees C. In vitro temperature-inducible C. albicans antigenic determinants reactive with secretory IgA were characterized by radioimmune Western blot as mannoproteins with molecular masses of 180-200, 130-150, 90-110, and 60-70 kDa. This is the first report on the expression of mannoproteins regulated by temperature involved in the secretory immune response during mucosal candidiasis.
authors.affiliation: Istituto di Microbiologia, Università degli Studi di Parma, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Polonelli
authors.forename: M
authors.initials: M
authors.lastname: Gerloni
authors.forename: S
authors.initials: S
authors.lastname: Conti
authors.forename: P
authors.initials: P
authors.lastname: Fisicaro
authors.forename: C
authors.initials: C
authors.lastname: Cantelli
authors.forename: P
authors.initials: P
authors.lastname: Portincasa
authors.forename: F
authors.initials: F
authors.lastname: Almondo
authors.forename: P L
authors.initials: PL
authors.lastname: Barea
authors.forename: F L
authors.initials: FL
authors.lastname: Hernando
authors.forename: J
authors.initials: J
authors.lastname: Ponton
mesh_terms: D000908:Antibodies, Fungal / Q000276:immunology*; D015153:Blotting, Western; D002177:Candidiasis / Q000276:immunology*; D002180:Candidiasis, Oral / Q000276:immunology; D002181:Candidiasis, Vulvovaginal / Q000276:immunology; D000939:Epitopes; D005260:Female; D005455:Fluorescent Antibody Technique; D006360:Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology*; D013696:Temperature
chemical_list: D000908:Antibodies, Fungal; D000939:Epitopes; D006360:Heat-Shock Proteins; D007071:Immunoglobulin A, Secretory; D008562:Membrane Glycoproteins; C025363:mannoproteins