title: Glutamate agonist-induced hippocampal lesion and nitric oxide synthase/NADPH-diaphorase: a light and electron microscopical study in the rat.
abstract: The 2-(2'-benzothiazolyl)-5-styryl-3-(4'-phthalhydrazidyl) tetrazolium chloride (BSPT)-tetrazolium salt technique for the electron microscopic demonstration of reduced nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) was used to localize nitric oxide synthase in the normal and excitotoxically lesioned rat hippocampus. The reaction product BSPT-formazan was shown to stain membranes predominantly of the endoplasmic reticulum. Apart from singular heavily labeled interneurons, the majority of neurons including pyramidal and granular cells and a few astroglial cells, light microscopically 'unstained', showed labeled membrane portions, but to a by far lesser extent. In lesioned areas some prominantly stained neurons rich in labeled membranes and surrounded by cell debris seemed to be largely preserved. An increased number of ultrahistochemically NADPH-d-stained glial cells, in particular astrocytes, was seen.
authors.affiliation: Institute of Medical Neurobiology, Medical Academy of Magdeburg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Wolf
authors.forename: G
authors.initials: G
authors.lastname: Henschke
authors.forename: S
authors.initials: S
authors.lastname: Würdig
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D000818:Animals; D052160:Benzothiazoles; D001927:Brain Diseases / Q000139:chemically induced* / Q000378:metabolism; D005971:Glutamates / Q000502:physiology; D018698:Glutamic Acid; D006624:Hippocampus / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009252:NADPH Dehydrogenase / Q000032:analysis*; D009474:Neurons / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology*; D019001:Nitric Oxide Synthase; D017378:Quinolinic Acid; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D013778:Tetrazolium Salts; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
chemical_list: D052160:Benzothiazoles; D005971:Glutamates; D016194:Receptors, N-Methyl-D-Aspartate; D013778:Tetrazolium Salts; D009569:Nitric Oxide; C024091:BSPT salt; D018698:Glutamic Acid; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase; D017378:Quinolinic Acid