Cloning and characterization of cDNAs encoding two normal isoforms of bovine stem cell factor.
The cDNA clones encoding two isoforms of bovine stem cell factor (bSCF) were obtained using reverse transcriptase-polymerase chain reaction, and their sequences were determined. The deduced amino acid sequences of the longer and shorter isoforms of bSCF consist, respectively, of 274 and 246 residues and show a high degree of identity to those of SCFs of different animal species. Northern blot analysis with the cDNA revealed the expression of a 5.8 kilobase bSCF RNA in fetal bovine tissues.
Zhou
J H
JH
Institute of Society for Techno-innovation of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
Hikono
H
H
Ohtaki
M
M
Kubota
T
T
Sakurai
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D016298:Hematopoietic Cell Growth Factors / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D019089:Stem Cell Factor
D018076:DNA, Complementary; D016298:Hematopoietic Cell Growth Factors; D019089:Stem Cell Factor