[Effects of thromboxane A2 and prostaglandin I2 on the release of chemical mediators from human peripheral leukocytes].
To investigate the effects of thromboxane (Tx) A2 and prostaglandin (PG) I2 on the release of chemical mediators, we studied the effects of TxA2 mimetic U-46619 and PGI2 on the release of histamine (HA), leukotriene (LT) C4/D4/E4 (peptide-LTs) and LTB4 from stimulated human peripheral leukocytes. We measured the levels of those mediators in the supernatant fluid from leukocyte suspensions stimulated by calcium ionophore A23187 (Ca-I, 10(-6) M) with U-46619 or PGI2. U-46619 did not affect the release of HA, peptide-LTs or LTB4 from leukocytes stimulated by Ca-I. The release of HA from leukocytes induced by Ca-I was inhibited significantly by PGI2 (p < 0.05). PGI2 also significantly inhibited the release of peptide-LTs (p < 0.05) and LTB4 (p < 0.01) from leukocytes. These results indicate that TxA2, an arachidonic acid cyclooxygenase metabolite does not affect the release of HA, peptide-LTs and LTB4 from leukocytes, but that PGI2 inhibits the release of these mediators induced by Ca-I.
Tanaka
Y
Y
Department of Analytical Chemistry, School of Pharmaceutical Sciences, Showa University.
Imai
T
T
Horikoshi
S
S
Kurokawa
M
M
Kimura
T
T
Matsuura
T
T
Idaira
K
K
Adachi
M
M
Takahashi
T
T
D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D000328:Adult; D011464:Epoprostenol / Q000494:pharmacology*; D005260:Female; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007962:Leukocytes / Q000187:drug effects* / Q000378:metabolism; D015289:Leukotrienes / Q000378:metabolism*; D008297:Male; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology*; D013928:Thromboxane A2 / Q000031:analogs & derivatives* / Q000494:pharmacology*
D015289:Leukotrienes; D011450:Prostaglandin Endoperoxides, Synthetic; D013928:Thromboxane A2; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D011464:Epoprostenol