Expression of myelin-associated glycoprotein isoforms after sciatic nerve crush injury in mice.
The large myelin-associated glycoprotein isoform (L-MAG) protein and small myelin-associated glycoprotein isoform (S-MAG) protein were demonstrated after sciatic nerve crush injury in mice by an immunoblotting technique using specific antibodies to the L-MAG protein and the S-MAG protein, respectively. Immunoblots indicated a rapid decrease in expression of both isoform proteins in the crushed sciatic nerves to < 10% of the control side. By 13 d after injury, L-MAG protein expression had quickly recovered to 100% of the control level. Following the increase in L-MAG protein expression, S-MAG protein expression recovered to 100% by 20 d after injury. It has been reported that the developmental maximum expression of L-MAG protein precedes that of S-MAG protein in both central and peripheral nervous system (CNS and PNS). Our previous work demonstrated that L-MAG mRNA was characteristically induced at the time of most active myelination, including remyelination in the CNS. We here have shown the expression of L-MAG protein precedes that of S-MAG protein during active remyelination in the PNS. This suggests that it plays an important role in the early stage of myelin formation.
Inuzuka
T
T
Department of Neurology, Niigata University, Japan.
Sato
S
S
Fujita
N
N
Baba
H
H
Nakano
R
R
Ishiguro
H
H
Miyatake
T
T
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007536:Isomerism; D051379:Mice; D008815:Mice, Inbred Strains; D009185:Myelin Proteins / Q000096:biosynthesis* / Q000276:immunology; D009186:Myelin Sheath / Q000378:metabolism / Q000502:physiology; D018992:Myelin-Associated Glycoprotein; D009409:Nerve Crush; D017933:Peripheral Nervous System / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism*
D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D012333:RNA, Messenger