Antibodies against terminal galactosyl alpha(1-3) galactose epitopes in patients with idiopathic myelofibrosis.
Sera from patients with myelofibrosis were analysed for circulating antibodies against an antigenic determinant characterized by two molecules of galactose in alpha 1-3 linkage (anti-Gal antibodies). 50% of patients were found to have values above the 90th percentile of the values of control sera chosen as a cut-off. The median level of the antibodies was significantly higher than the value detected in normal controls, but no difference could be found between patients with idiopathic myelofibrosis and those with myelofibrosis associated to a chronic myeloproliferative disorder. Anti-Gal antibodies were found to correlate with disease activity and with platelet count whereas no correlation was detected with other haematological parameters. Furthermore, for evaluation of disease activity, determination of serum anti-Gal antibodies was a sensitive and specific parameter. We conclude that humoral immunity against Gal alpha 1-3Gal may provide a sensitive tool to detect disease activity in patients with idiopathic myelofibrosis and may be important for understanding its pathogenesis.
Leoni
P
P
Istituto di Clinica Medica Generale e Terapia Medica, Università di Ancona, Italy.
Rupoli
S
S
Salvi
A
A
Sambo
P
P
Cinciripini
A
A
Gabrielli
A
A
D000368:Aged; D001323:Autoantibodies / Q000032:analysis*; D002908:Chronic Disease; D004187:Disaccharides / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009196:Myeloproliferative Disorders / Q000150:complications; D055728:Primary Myelofibrosis / Q000209:etiology / Q000276:immunology*
D001323:Autoantibodies; D004187:Disaccharides; D000939:Epitopes; C055075:galactosyl-(1-3)galactose