Seven transmembrane domain receptor subtypes identified in NG108-15 cells by reverse transcription-polymerase chain reaction.
NG108-15 neuroblastoma x glioma cells are widely used for the study of neurotransmitter receptors. We utilized reverse transcription-polymerase chain reaction to amplify members of the seven transmembrane domain class of G-protein linked receptors using RNA isolated from NG108-15 cells. Two complementary DNAs representing receptors were obtained; based upon comparison with the sequence database, they probably represent the murine dopamine D1A receptor and a receptor closely related to the serotonin 5HT1D receptor subtype. The finding of the 5HT receptor subtype is of interest, as only the 5HT3 subtype was previously identified in NG108-15 cells by pharmacological means. Certain responses of NG108-15 cells to serotonin have been described that do not appear to be mediated by known 5HT receptor subtypes. The cDNA we cloned may therefore represent an additional 5HT1D subclass.
Chan
R J
RJ
Department of Medicine, Indiana University School of Medicine, Indianapolis 46202-5121.
McBride
A W
AW
Crabb
D W
DW
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D004285:Dogs; D019204:GTP-Binding Proteins / Q000378:metabolism; D005910:Glioma; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D008969:Molecular Sequence Data; D009447:Neuroblastoma; D016133:Polymerase Chain Reaction / Q000379:methods; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D017447:Receptors, Dopamine D1 / Q000737:chemistry*; D011985:Receptors, Serotonin / Q000096:biosynthesis* / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D017447:Receptors, Dopamine D1; D011985:Receptors, Serotonin; D012194:RNA-Directed DNA Polymerase; D019204:GTP-Binding Proteins