Quantification of urinary catecholamines, their abundant metabolites, and 5-hydroxyindoleacetic acid by high performance liquid chromatography and electrochemical detection, using a single mobile phase and uniform isocratic conditions.
A simple and flexible isocratic HPLC procedure was developed for the measurement of catecholamines and their abundant metabolites ( e.g. nor- metanephrine, metanephrine, methoxy-tyramine, vanillylmandelic acid, homovanillic acid) and 5-hydroxyindolacetic acid by ion-pair reversed phase chromatography on C18 columns, using a single mobile phase containing both sodium octane sulphonate and diethylamine as ion-pairing reagents.
Radjaipour
M
M
Medizinische Universitätsklinik, Abt. IV, Tübingen, Germany.
Raster
H
H
Liebich
H M
HM
D002395:Catecholamines / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D004298:Dopamine / Q000031:analogs & derivatives / Q000652:urine; D004563:Electrochemistry; D006719:Homovanillic Acid / Q000652:urine; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000652:urine*; D008676:Metanephrine / Q000652:urine; D009647:Normetanephrine / Q000652:urine; D012680:Sensitivity and Specificity; D014642:Vanilmandelic Acid / Q000652:urine
D002395:Catecholamines; D009647:Normetanephrine; D008676:Metanephrine; D006897:Hydroxyindoleacetic Acid; D014642:Vanilmandelic Acid; C001746:3-methoxytyramine; D004298:Dopamine; D006719:Homovanillic Acid