B and T lymphocyte subsets enter peripheral lymph nodes and Peyer's patches without preference in vivo: no correlation occurs between their localization in different types of high endothelial venules and the expression of CD44, VLA-4, LFA-1, ICAM-1, CD2 or L-selectin.
Many lymphocytes enter tissues such as peripheral lymph nodes, and Peyer's patches through high endothelial venules (HEV). It is known that HEV differ in the expression of adhesion molecules as lymphocyte subsets do. Through the interaction of these molecules B and T lymphocyte subsets are thought to be preferentially directed into lymphoid organs. However, it is unclear which role these mechanisms play in vivo, since there are no studies demonstrating that blood lymphocyte subsets preferentially interact with different types of HEV in vivo. Therefore, in the present study the frequency of B, T, CD4+ and CD8+ lymphocytes in the wall of the HEV of rat peripheral lymph nodes and Peyer's patches was analyzed by immunohistology. In addition, the expression of CD44, VLA-4, LFA-1, ICAM-1, CD2 and L-selectin on B and T lymphocyte subsets of the blood was determined by flow cytometry. Although B and T lymphocytes showed significantly different levels of expression for each adhesion molecule investigated, the relation of B and T lymphocytes within the HEV of peripheral lymph nodes and Peyer's patches was strikingly comparable (38.0 +/- 5.2% vs. 40.6 +/- 5.7% and 62.0 +/- 5.2% vs. 59.4 +/- 5.7%, respectively). The same was true for CD4+ and CD8+ cells. Thus, although HEV and the blood lymphocyte subsets differ markedly in their expression pattern of adhesion molecules, the existing levels are sufficient to mediate comparable entrance of B and T lymphocyte subsets into both types of HEV.
Westermann
J
J
Zentrum Anatomie Abt. II, Medizinische Hochschule Hannover, Germany.
Nagahori
Y
Y
Walter
S
S
Heerwagen
C
C
Miyasaka
M
M
Pabst
R
R
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000166:cytology*; D018801:CD2 Antigens / Q000502:physiology; D002352:Carrier Proteins / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000502:physiology*; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000276:immunology; D018960:Hyaluronan Receptors; D018799:Intercellular Adhesion Molecule-1 / Q000502:physiology; D019041:L-Selectin; D008198:Lymph Nodes / Q000166:cytology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology; D008297:Male; D010581:Peyer's Patches / Q000166:cytology*; D051381:Rats; D011917:Rats, Inbred Lew; D011956:Receptors, Cell Surface / Q000502:physiology; D016201:Receptors, Lymphocyte Homing / Q000502:physiology; D016029:Receptors, Very Late Antigen / Q000502:physiology; D016176:T-Lymphocyte Subsets / Q000166:cytology*
D018801:CD2 Antigens; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D016169:Lymphocyte Function-Associated Antigen-1; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D016029:Receptors, Very Late Antigen; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin