Characterization of eight endogenous viral (ev) genes of meat chickens in semi-congenic lines.
Restriction fragment length polymorphism analysis was conducted for a set of eight different meat chicken-derived endogenous viral genes (ev genes) of the avian leukosis viral (ALV) family. Each viral element was first isolated into a separate single-element line by selective breeding. Genomic DNA from the founder male for each semi-congenic, single-element line was digested with each of four restriction enzymes, and the resulting Southern blots were each hybridized with up to four probes representing different portions of the ALV retroviral genome. Among the eight elements, there was one that represents the broiler equivalent of locus ev3 of White Leghorn chickens. A second broiler element showed a SacI-specific junction fragment similar to that of ev8. The remainder appeared to be different from any of the 21 ev genes previously described for White Leghorn chickens. Four of the eight elements examined were essentially complete, but the rest have sustained internal deletions.
Grunder
A A
AA
Centre for Food and Animal Research, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.
Benkel
B F
BF
Chambers
J R
JR
Sabour
M P
MP
Gavora
J S
JS
D001355:Alpharetrovirus / Q000235:genetics*; D000818:Animals; D002645:Chickens / Q000821:virology*; D005260:Female; D017353:Gene Deletion; D005814:Genes, Viral / Q000235:genetics*; D008297:Male; D012150:Polymorphism, Restriction Fragment Length