title: Isolation and characterization of the major equilibrium product of FK-520.
abstract: The existence of an isomeric form in equilibrium with the major component of FK-520 in polar solutions has been demonstrated. This minor component has been isolated in high yield and purity by a novel crystallization strategy and preparative HPLC. The equilibrium product was characterized by NMR and MS.
authors.affiliation: Merck Research Laboratories, Rahway, NJ 07065-0900.
authors.forename: F P
authors.initials: FP
authors.lastname: Gailliot
authors.forename: T K
authors.initials: TK
authors.lastname: Natishan
authors.forename: J M
authors.initials: JM
authors.lastname: Ballard
authors.forename: R A
authors.initials: RA
authors.lastname: Reamer
authors.forename: D
authors.initials: D
authors.lastname: Kuczynski
authors.forename: G J
authors.initials: GJ
authors.lastname: Mcmanemin
authors.forename: R S
authors.initials: RS
authors.lastname: Egan
authors.forename: B C
authors.initials: BC
authors.lastname: Buckland
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D007166:Immunosuppressive Agents / Q000737:chemistry* / Q000302:isolation & purification*; D007536:Isomerism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet; D016559:Tacrolimus / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D007166:Immunosuppressive Agents; C058028:immunomycin; D016559:Tacrolimus