title: Assessment of myointimal cellular kinetics in a model of angioplasty by means of proliferating cell nuclear antigen expression.
abstract: A detailed temporal assessment of cellular proliferation was carried out by means of immunostaining for proliferating cell nuclear antigen in a normolipemic rabbit model of balloon angioplasty to the iliac arteries. Assessment was made at 30 minutes, 2 hours, 1 day, 3 days, 7 days, 14 days, 1 month and 3 months after the procedure. Intimal hyperplasia was first noted at day 3; a prominent layer was formed by day 14. Cellular proliferation in the vessel media was observed as early as day 1 (percentage of positive-staining cells 0.5% +/- 0.2%), reaching a maximum by day 7 (16.9% +/- 5.1%) before returning to baseline levels by 1 month (0.2% +/- 0.02%); in the intima, cellular proliferation was first noted at day 7 (0.7% +/- 0.3%) and reached a maximum at day 14 (4.1% +/- 0.4%) before returning to baseline levels at 1 month (0.3% +/- 0.1%). Use of proliferating cell nuclear antigen expression in this model of angioplasty provided a simple and reproducible method of assessing cellular proliferation after vascular injury and may prove useful for monitoring the effects, in experimental models, of agents for reducing myointimal hyperplasia.
authors.affiliation: Academic Department of Cardiology, Glenfield General Hospital, Leicester, United Kingdom.
authors.forename: R S
authors.initials: RS
authors.lastname: More
authors.forename: G
authors.initials: G
authors.lastname: Rutty
authors.forename: M J
authors.initials: MJ
authors.lastname: Underwood
authors.forename: M J
authors.initials: MJ
authors.lastname: Brack
authors.forename: A H
authors.initials: AH
authors.lastname: Gershlick
mesh_terms: D000800:Angioplasty, Balloon / Q000009:adverse effects; D000818:Animals; D001324:Autoantigens / Q000032:analysis*; D002452:Cell Count; D002453:Cell Cycle; D002455:Cell Division; D006965:Hyperplasia; D007150:Immunohistochemistry; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D011817:Rabbits; D013194:Staining and Labeling; D017539:Tunica Intima / Q000166:cytology* / Q000473:pathology; D017540:Tunica Media / Q000276:immunology / Q000473:pathology*
chemical_list: D001324:Autoantigens; D018809:Proliferating Cell Nuclear Antigen