Effect of hyaluronidase on brain extracellular matrix in vivo and optic nerve regeneration.
In the rat, intracerebral injection of bacterial hyaluronidase resulted in the almost complete disappearance of hyaluronic acid (HA) and glial hyaluronate-binding protein (GHAP) from cerebral hemispheres, brain stem, and cerebellum (but not from optic nerves and chiasm) starting 2-3 hr after the injection. HA and GHAP reappeared throughout the brain in characteristic patches 2-3 days after the injection. The patches gradually became confluent and after 12 days the brain appeared virtually normal. In normal rat optic nerve, staining for HA and GHAP ceased abruptly in the region of the lamina cribrosa. The retina was completely negative. HA and GHAP disappeared from hyaluronidase-injected optic nerve, chiasm, and contralateral optic nerve. In hyaluronidase-injected crushed optic nerves, regenerated axons were able to grow for short distances (about 500 microns) into the distal stump undergoing Wallerian degeneration. No such growth was observed in saline-injected controls.
Tona
A
A
Department of Veterans Affairs Medical Center, West Roxbury, Massachusetts 02132.
Bignami
A
A
D000818:Animals; D001369:Axons / Q000502:physiology; D001921:Brain / Q000166:cytology / Q000187:drug effects*; D001923:Brain Chemistry / Q000187:drug effects; D002352:Carrier Proteins / Q000096:biosynthesis; D005109:Extracellular Matrix / Q000187:drug effects*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology / Q000378:metabolism; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid / Q000378:metabolism; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology*; D008297:Male; D009409:Nerve Crush; D009416:Nerve Regeneration / Q000187:drug effects*; D016900:Neurofilament Proteins / Q000276:immunology / Q000378:metabolism; D009900:Optic Nerve / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D016201:Receptors, Lymphocyte Homing / Q000096:biosynthesis
D002352:Carrier Proteins; D005904:Glial Fibrillary Acidic Protein; D018960:Hyaluronan Receptors; D016900:Neurofilament Proteins; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase