title: Human platelet antigen-1 (Zw) typing of fetuses by analysis of polymerase chain reaction-amplified genomic DNA from amniocytes.
abstract: Prenatal typing for the human platelet antigens-1 (HPA) permits identification of a fetus at risk for neonatal alloimmune thrombocytopenia (NAITP) in cases of HPA-1 incompatibility in which the father is heterozygous for the HPA-1a antigen. Diagnostic cordocentesis and phenotyping of the fetal platelets are used for this purpose. We applied allele-specific restriction enzyme analysis on polymerase chain reaction (PCR)-amplified DNA purified from amniocytes. This assays allows early second trimester typing for HPA-1 alleles. We were able to determine the genotype of three fetuses at risk. Iatrogenic fetal loss is lower with amniocentesis than with cordocentesis. Therefore, this technique is a welcome addition to the antenatal management of NAITP.
authors.affiliation: Department of Immunological Haematology, University of Amsterdam, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: Simsek
authors.forename: G C
authors.initials: GC
authors.lastname: Christiaens
authors.forename: H H
authors.initials: HH
authors.lastname: Kanhai
authors.forename: J R
authors.initials: JR
authors.lastname: Beekhuis
authors.forename: P M
authors.initials: PM
authors.lastname: Bleeker
authors.forename: A B
authors.initials: AB
authors.lastname: Vlekke
authors.forename: R
authors.initials: R
authors.lastname: Goldschmeding
authors.forename: A E
authors.initials: AE
authors.lastname: von dem Borne
mesh_terms: D000483:Alleles; D000649:Amniocentesis; D000653:Amniotic Fluid / Q000166:cytology*; D016824:Antigens, Human Platelet / Q000235:genetics / Q000276:immunology*; D001788:Blood Grouping and Crossmatching; D002478:Cells, Cultured; D017218:Cordocentesis; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D006801:Humans; D007114:Immunization; D039661:Integrin beta3; D007518:Isoantibodies / Q000097:blood*; D008297:Male; D010375:Pedigree; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D011296:Prenatal Diagnosis; D012306:Risk; D012449:Safety; D013921:Thrombocytopenia / Q000235:genetics / Q000276:immunology / Q000517:prevention & control*
chemical_list: D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D039661:Integrin beta3; D007518:Isoantibodies; D004247:DNA; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific