title: Involvement of ecto-ATPase as an ATP receptor in the stimulatory effect of extracellular ATP on NO release in bovine aorta endothelial cells.
abstract: The secretion of nitric oxide (NO) was stimulated by the addition of ATP or ADP, but not by AMP or adenosine, in cultured bovine aorta endothelial cells. Inhibitors of ecto-ATPase, NaN3 and Ap5A, significantly inhibited the stimulation, while an inhibitor of P2Y-purinoceptor and ecto-ATPase, RB2, completely suppressed it. A non-hydrolyzable ATP analogue, AMP-PNP, stimulated NO release; the stimulation was completely suppressed by RB2 but not by NaN3 and Ap5A. Therefore, only P2Y-purinoceptor was involved in the stimulation by AMP-PNP, while both ecto-ATPase and P2Y-purinoceptor were involved in the stimulation by ATP and ADP. It is not clear whether the stimulation is dependent on the dephosphorylation activity of ecto-ATPase or not, but the enzyme appears to act as an ATP and ADP receptor for signal transduction through adenine nucleotides.
authors.affiliation: Faculty of Pharmaceutical Sciences, Osaka University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yagi
authors.forename: I
authors.initials: I
authors.lastname: Nishino
authors.forename: M
authors.initials: M
authors.lastname: Eguchi
authors.forename: M
authors.initials: M
authors.lastname: Kitagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Miura
authors.forename: T
authors.initials: T
authors.lastname: Mizoguchi
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism* / Q000494:pharmacology*; D000266:Adenylyl Imidodiphosphate / Q000494:pharmacology; D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001011:Aorta; D001386:Azides / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D015226:Dinucleoside Phosphates / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D019001:Nitric Oxide Synthase; D019810:Sodium Azide; D014227:Triazines / Q000494:pharmacology
chemical_list: D001386:Azides; D015226:Dinucleoside Phosphates; D014227:Triazines; D000266:Adenylyl Imidodiphosphate; C012275:P(1),P(5)-di(adenosine-5'-)pentaphosphate; C012842:Cibacron Blue F 3GA; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D019810:Sodium Azide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D000251:Adenosine Triphosphatases; C027201:ectoATPase