Identification and carbohydrate specificity of a chicken serum mannan-binding protein reactive with a Ra chemotype strain of Salmonella typhimurium.
To compare a chicken serum mannan-binding protein (MBP) with a chicken Ra-reactive factor (RaRF), both serum proteins were isolated by combination of different chromatography. In SDS-PAGE, both purified proteins were resolved into a major protein band with a molecular weight of 34,000 and a few minor protein bands. In Western blotting with both purified proteins, only a single band with a molecular weight of 34,000 was detected with anti-chicken serum MBP antibody, indicating that the major protein band of chicken serum MBP and RaRF are antigenically identical. The combining sites of these two proteins were most reactive with N-acetylmannosamine followed by mannose, N-acetylglucosamine, and L-fucose. These findings suggest that chicken serum MBP may be identical to its corresponding bactericidal factor RaFR in terms of antigenicity and monosaccharide specificity.
Sugii
S
S
Department of Serology and Immunology, School of Medical Technology, Kitasato University, Kanagawa, Japan.
Hirota
Y
Y
D000209:Acute-Phase Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D000941:Antigens; D050260:Carbohydrate Metabolism; D002352:Carrier Proteins / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D002645:Chickens / Q000097:blood*; D037222:Collectins; D008351:Mannans / Q000378:metabolism*; D011485:Protein Binding; D012486:Salmonella typhimurium / Q000378:metabolism*
D000209:Acute-Phase Proteins; D000941:Antigens; D002352:Carrier Proteins; D037222:Collectins; D008351:Mannans