[Oligodeoxyribonucleotides complementary to sections of the mollicute ribosomal operon as transcription inhibitors in vitro].
Antisense oligodeoxyribonucleotides have been studied for their effect on the transcription in vitro in mollicutes. A synthetic fragment of DNA [symbol: see text] complementary to that part of DNA which codes the 1510-1521 area of the 3'-terminal sequence 16S-pRNA of all mollicutes was used in the study as well as its modifications by imidasophenasine derivatives: [symbol: see text], [symbol: see text] [symbol: see text]. Maximal inhibition of the mollicute transcription in vitro was observed with 100 nM oligonucleotide concentration. Lower or higher concentrations were less effective. Transcription initiated by RNA-polymerase of M. fermentans PG-18 (a mollicute strain referring to AIDS disease) proved to be the most sensitive to the effect of modified oligonucleotide: it was inhibited by 75-80%. It is concluded that modified oligonucleotides exert a dual effect on transcription: firstly, they participate in nonspecific interaction with RNA-polymerase which induces insignificant inhibition of transcription and, secondly, they complementary interact with homologous sections of one-stranded DNA-matrix and block the RNA synthesis. Binding of modified oligonucleotides with DNA is rather strong.
Babichev
V V
VV
Skripal'
I G
IG
Bezuglyĭ
S V
SV
Panchenko
L P
LP
Shalamaĭ
A S
AS
Makitruk
B L
BL
Goncharenko
V S
VS
Shimko
N N
NN
D000128:Acholeplasma laidlawii / Q000378:metabolism*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism*; D008969:Molecular Sequence Data; D016858:Mycoplasma fermentans / Q000378:metabolism*; D009177:Mycoplasma pneumoniae / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000378:metabolism*; D009876:Operon / Q000502:physiology*; D012329:RNA, Bacterial / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*
D004269:DNA, Bacterial; D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D012329:RNA, Bacterial