title: Haemopoietic progenitor cells are reduced in aplastic anaemia.
abstract: We investigated the frequencies of early populations of progenitors in aplastic anaemia (AA) bone marrow, from patients with a range of disease severity, compared with normal. Double-colour immunofluorescent staining for CD34 and CD33 was carried out on bone marrow mononuclear cells (BMMC) and analysed using fluorescence activated cell sorting (FACS). AA CD34+ cells were reduced by 68% compared to normal. In addition, AA CD33+ cells and the three progenitor subsets (CD34+/CD33-, CD34+/CD33+ and CD34-/CD33+) were reduced by 44-80%. Our data lend further support for an early stem cell deficiency in AA.
authors.affiliation: Department of Cellular and Molecular Sciences, St George's Hospital Medical School, London.
authors.forename: J
authors.initials: J
authors.lastname: Scopes
authors.forename: M
authors.initials: M
authors.lastname: Bagnara
authors.forename: E C
authors.initials: EC
authors.lastname: Gordon-Smith
authors.forename: S E
authors.initials: SE
authors.lastname: Ball
authors.forename: F M
authors.initials: FM
authors.lastname: Gibson
mesh_terms: D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000473:pathology*; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology*; D002648:Child; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D063268:Sialic Acid Binding Ig-like Lectin 3
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic; C571163:CD33 protein, human; D063268:Sialic Acid Binding Ig-like Lectin 3