[Dynamics of antibody formation, isotypes and specificity of antibodies in the immune response of Balb/c mice to cytochrome c].
The production of IgG antibody (Ab) to cytochrome c in BALB/c mice reached its maximum four weeks after primary immunization which is significantly later than the response to the same dose of keyhole limpet hemocyanin (KLH). The relative amounts of IgG subclasses varied during the immune response to KLH: IgG2a Abs dominating on day 11 were replaced by IgG1 on day 30 after immunization. This was not the case with anti-cytochrome c Abs: their IgG subclasses varied significantly among individual mice within the strain. The heme elimination altered the cytochrome c antigenic structure, although some antigenic determinants remained intact. The heme with its neighbouring residues represented by heme peptide 14-22 was not recognized by anti-cytochrome c Abs. Serum IgG Abs interacted with peptides 1-65 and 1-80 obtained by BrCN hydrolysis of cytochrome c; however, they did not bind to peptide 66-104. The Abs affinity purified on cytochrome c bound to peptides 1-65 and 1-80, while the Abs purified on peptide 1-65 or apo-cytochrome could recognize native cytochrome c as well.
Kavun
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D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000941:Antigens / Q000276:immunology; D003574:Cytochrome c Group / Q000276:immunology* / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology
D000941:Antigens; D003574:Cytochrome c Group; D000939:Epitopes; D007074:Immunoglobulin G; D010446:Peptide Fragments; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin