The use of random-breakage mapping to locate the genes APN1 and YUH1 in the Saccharomyces genome, and to determine gene order near the left end of chromosome XI.
We have used the previously described technique of random-breakage mapping to locate the two yeast genes APN1 and YUH1. The APN1 locus is located approximately 235 kb from the left telomere of chromosome XI, and shows weak (approximately 53 cM) genetic linkage to ura1. The YUH1 locus is located approximately 140 kb from the right telomere of chromosome X, and genetically maps 3.6 cM distal to cdc11. In addition, we show by random-breakage mapping that TRP3 is located approximately 45 kb from the left telomere of chromosome XI, whereas FAS1 is approximately 110 kb from the same telomere. This supports a gene order on the left distal portion of chromosome XI that agrees with other physical reports but is inverted with respect to Edition 11 of the published genetic map. This report confirms that random-breakage mapping is a rapid and convenient method of locating cloned genes.
Game
J
J
Life Sciences Division, Lawrence Berkeley Laboratory, Berkeley, CA 94720.
Bell
M
M
Ramotar
D
D
Miller
H
H
D002874:Chromosome Mapping / Q000379:methods*; D015825:Chromosomes, Fungal / Q000528:radiation effects; D003001:Cloning, Molecular; D004249:DNA Damage; D004271:DNA, Fungal / Q000235:genetics / Q000528:radiation effects; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004706:Endodeoxyribonucleases / Q000235:genetics*; D010450:Endopeptidases / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal; D005656:Fungal Proteins; D004706:Endodeoxyribonucleases; D043223:Deoxyribonuclease IV (Phage T4-Induced); D010450:Endopeptidases; C059237:ubiquitin-Nalpha-protein hydrolase; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase