Lymphocyte-synovial cell interactions: a role for beta 1 and beta 3 integrin-mediated adhesion to cellular fibronectin.
The interactions of lymphocytes with cultured synovial cells derived from rheumatoid arthritis patients were examined. A number of lymphoid cell lines bound to these cells. The adherence of several of these lines was inhibited by antibodies to fibronectin. The adherence of the T cell leukemia Jurkat was sensitive to inhibition by antibodies to the fibronectin receptors alpha 4 beta 1 and alpha 5 beta 1. The adherence of the beta 1 integrin negative B cell line RPMI 8866 was inhibited by antibodies to the vitronectin receptor, alpha v beta 3. The interactions of several other cell lines with synovial cells appeared to be independent of this fibronectin-dependent pathway. These results indicate that multiple potential adhesion pathways for cellular interactions in the tissues may exist. The adherence to cell-associated fibronectin may play a contributory role in such processes for certain lymphocyte subsets.
Wilkins
J
J
Rheumatic Diseases Unit Research Laboratory, University of Manitoba, Winnipeg, Canada.
Lyttle
D
D
Sivananthan
K
K
el-Gabalawy
H
H
D002448:Cell Adhesion / Q000276:immunology*; D002478:Cells, Cultured; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D019012:Integrin beta1; D039661:Integrin beta3; D016023:Integrins / Q000502:physiology*; D008214:Lymphocytes / Q000502:physiology*; D011485:Protein Binding; D013583:Synovial Membrane / Q000166:cytology*; D014407:Tumor Cells, Cultured
D005353:Fibronectins; D019012:Integrin beta1; D039661:Integrin beta3; D016023:Integrins