A new biological activity for the neuropeptide FMRFamide: experimental evidence for a secretagogue effect on amylase secretion in the scallop Pecten maximus.
FMRFamide immunoreactivity in the digestive tract of the bivalve mollusc Pecten maximus was investigated by immunocytochemistry. Positive FMRFamide-like immunoreactivity was detected in nerve fibres in close contact with exocrine alpha amylase secreting cells. Physiological studies on enzymatically dissociated cells of the stomach-digestive gland complex demonstrated the involvement of FMRFamide and analogues in the control of alpha amylase release from the cells. The FMRFamide-induced secretion was shown to be time- and dose-dependent. In contrast to most naturally occurring vertebrate secretagogues which are hormones, FMRFamide appears to work in our in vitro system as a paracrine factor.
Favrel
P
P
Laboratoire de Biologie et Biotechnologies Marines, IBBA, IFREMER Unité de Recherches Marines 14, Université de Caen, France.
Giard
W
W
Benlimane
N
N
Boucaud-Camou
E
E
Henry
M
M
D000595:Amino Acid Sequence; D000681:Amylases / Q000378:metabolism*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D019835:FMRFamide; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D008974:Mollusca / Q000378:metabolism*; D009479:Neuropeptides / Q000032:analysis / Q000494:pharmacology*
D009479:Neuropeptides; D019835:FMRFamide; D000681:Amylases