Production of monoclonal antibodies recognising different epitopes present on insulin-like growth factor 1.
Monoclonal antibodies (MAbs) were generated against recombinant human insulin-like growth factor 1 (IGF-1) by fusion of NS-1 myeloma cells with spleen cells from BALB/c X DBA mice immunised with recombinant IGF-1 and synthetic peptide sequences derived from the published amino acid sequence of IGF-1. MAbs were produced that recognised four distinct epitopes including two defined segments of the C and D domains. All MAbs were IgM mouse immunoglobulins. These results indicate the feasibility of producing MAbs to highly conserved proteins.
Harvey
D
D
Horticulture and Food Research Institute of New Zealand Limited, Batchelar Research Centre, Palmerston North.
Jones
W T
WT
Jones
S D
SD
Ryan
G B
GB
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007334:Insulin-Like Growth Factor I