title: Presentation of soluble and bacterial antigens by milk-derived cells to unprimed bovine T cells in vitro.
abstract: The ability of cells isolated from bovine milk and peripheral blood to present soluble protein and particulate bacterial antigens to peripheral blood T lymphocytes was compared using a culture system which consistently supports antigen-specific, primary, proliferative responses. The present study shows that cells from blood and from milk can present antigen to unprimed T cells. Major histocompatibility complex class II restriction of the responses was demonstrated by abrogation of proliferation by the addition of anti-bovine class II monoclonal antibody to cultures. Although cells derived from blood or milk were shown to be capable of presenting antigen to T cells, differences in optimal culture conditions and kinetics of the resulting response were observed.
authors.affiliation: Department of Veterinary Medicine, University of Bristol, School of Veterinary Science, Langford, UK.
authors.forename: J L
authors.initials: JL
authors.lastname: Fitzpatrick
authors.forename: N A
authors.initials: NA
authors.lastname: Williams
authors.forename: M
authors.initials: M
authors.lastname: Bailey
authors.forename: P W
authors.initials: PW
authors.lastname: Bland
authors.forename: C R
authors.initials: CR
authors.lastname: Stokes
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D008892:Milk / Q000166:cytology*; D012995:Solubility; D013291:Streptococcus / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D000942:Antigens, Bacterial; D000949:Histocompatibility Antigens Class II