Anti-GMP140 (CD62) autoantibody in a patient with autoimmune thrombocytopenic purpura.
The presence of platelet GMP140 (CD62) antibodies was analysed by the MAIPA test in 57 sera from patients with AITP and on platelets from 54 patients with thrombocytopenia of suspected immune origin. A CD62 antibody was found in only one serum. Its specificity was confirmed by an ELISA and a radioimmunoprecipitation procedure using total intact platelets and immuno-purified GMP140. An increased amount of platelet-associated (PA) IgG, due to in vivo fixation of GMP140 and GpIIb/IIIa antibodies, was also found on the patient platelet membrane. suggesting that GMP140 autoantibody may contribute to immune platelet destruction. No increase in PAGMP140 antibody was found on the other 54 platelet suspensions.
Bierling
P
P
Laboratoire d'Immunologie Leucoplaquettaire, Hôpital Henri Mondor, Créteil, France.
Bettaieb
A
A
Fromont
P
P
Favrin
M
M
Duedari
N
N
D000368:Aged; D016824:Antigens, Human Platelet / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis*; D001792:Blood Platelets / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000276:immunology*; D015531:Radioimmunoprecipitation Assay
D016824:Antigens, Human Platelet; D001323:Autoantibodies; D007074:Immunoglobulin G; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins