Dose response for heritable translocations induced by acrylamide in spermatids of mice.
A heritable translocation test was carried out with acrylamide (AA) to obtain a dose-response relationship for induction of reciprocal translocations in late spermatids of the mouse. Male C3H/E1 mice were treated with single i.p. doses of 50 and 100 mg/kg of acrylamide and mated 7-16 days after treatment to untreated female 102/E1 mice. Translocation carriers among the F1 progeny were selected by a sequential procedure of fertility testing and cytogenetic analysis including G-band karyotyping to determine the chromosomes involved in the respective translocations. The translocation frequencies observed with 50 mg/kg and 100 mg/kg of AA were 0.6% and 2.7%, respectively. The historical control translocation frequency was 0.04%. Doubling dose estimates based on these and previous data are discussed.
Adler
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Institut für Säugetiergenetik, GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Oberschleissheim, Germany.
Reitmeir
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Schmöller
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Schriever-Schwemmer
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D020106:Acrylamide; D000178:Acrylamides / Q000633:toxicity*; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D001854:Bone Marrow Cells; D004305:Dose-Response Relationship, Drug; D005260:Female; D007621:Karyotyping; D008098:Litter Size; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D011247:Pregnancy; D013087:Spermatids / Q000187:drug effects*; D014178:Translocation, Genetic / Q000187:drug effects*
D000178:Acrylamides; D009153:Mutagens; D020106:Acrylamide