Structural studies of the Escherichia coli O90 O-antigen polysaccharide.
The O-specific side-chain of the lipopolysaccharide from Escherichia coli O90 has been investigated using methylation analysis, partial hydrolysis, and NMR spectroscopy as the principal methods. It is concluded that the polysaccharide is composed of tetrasaccharide repeating-units having the following structure. [formula: see text] The polysaccharide contains approximately one mole of O-acetyl groups per repeating unit, located on the fucose residue.
Ratnayake
S
S
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
Widmalm
G
G
Weintraub
A
A
Medina
E C
EC
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry / Q000276:immunology*; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D019081:O Antigens; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial