title: Tachykinins induce a [Ca2+]i rise in the acinar cells of feline tracheal submucosal gland.
abstract: The intracellular Ca2+ concentration ([Ca2+]i) of acinar cells of isolated submucosal glands from trachea was measured using a fluorescent dye, Fura-2. Neurokinin A (NK-A) produced a sustained rise in [Ca2+]i in a dose-dependent manner, reaching a response of 500 to 600% of the prior baseline value at 10(-6) or 10(-5) M, and the NK-A evoked [Ca2+]i was significantly higher than that by substance P (SP) at similar concentrations. NK-B did not induce significant increases in [Ca2+]i. In a Ca(2+)-free solution, NK-A produced a transient rise in [Ca2+]i, which returned to the baseline within 3 min. Mucus glycoprotein (MGP) secretion, estimated by measuring trichloroacetic-acid (TCA) precipitable glycoconjugates, was stimulated by NK-A or SP. These findings indicate that tachykinins produce a rise in [Ca2+]i by both entry from the extracellular solution and release from intracellular storage, probably by NK-2 receptor stimulation, and stimulate MGP secretion from airway submucosal glands.
authors.affiliation: First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nagaki
authors.forename: H
authors.initials: H
authors.lastname: Ishihara
authors.forename: S
authors.initials: S
authors.lastname: Shimura
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: T
authors.initials: T
authors.lastname: Takishima
authors.forename: K
authors.initials: K
authors.lastname: Shirato
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002415:Cats; D005088:Exocrine Glands / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016257:Fura-2; D006023:Glycoproteins / Q000378:metabolism; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D009077:Mucins / Q000378:metabolism; D015288:Neurokinin A / Q000494:pharmacology; D015287:Neurokinin B / Q000494:pharmacology; D064729:Neurokinin-1 Receptor Antagonists; D010456:Peptides, Cyclic / Q000494:pharmacology; D018041:Receptors, Neurokinin-2 / Q000037:antagonists & inhibitors; D013373:Substance P / Q000037:antagonists & inhibitors / Q000494:pharmacology; D015320:Tachykinins / Q000494:pharmacology*; D014132:Trachea / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: D006023:Glycoproteins; D007202:Indicators and Reagents; D009077:Mucins; D064729:Neurokinin-1 Receptor Antagonists; D010456:Peptides, Cyclic; D018041:Receptors, Neurokinin-2; D015320:Tachykinins; C075570:FK 224; D013373:Substance P; D015288:Neurokinin A; D015287:Neurokinin B; D002118:Calcium; D016257:Fura-2