title: Genetic control of the antibody repertoire against excretory/secretory products and acetylcholinesterases of Dictyocaulus viviparus.
abstract: Outbred Dunkin-Hartley and inbred strain 2 and strain 13 guinea pigs were immunized with Dictyocaulus viviparus adult ES products prior to challenge with third stage larvae. Antibody responses of the three strains to adult ES products and the acetylcholinesterase (AChE) isoforms which they contain were examined. Using immunoprecipitation and ELISA, it was observed that responses in the three strains to adult ES products were distinct: considerable heterogeneity in the antibody repertoire was observed between outbred Dunkin-Hartley animals, with only slight variation occurring amongst the inbred individuals. Responses to the AChE isoforms were heterogeneous amongst individual outbred guinea pigs but were more consistent in inbred strain 2 and 13 animals in which strain-specific patterns of recognition were observed. Previous studies with nematode infections have indicated a role for the major histocompatibility complex in determining the nature and level of the immune response. As the inbred strains bear different alleles at the Class II region but are identical at the Class I region, the differences observed are likely to be due to genes mapping to the Class II locus. This is therefore the first report of genetic restriction of the antibody repertoire to secreted AChEs of a parasitic nematode.
authors.affiliation: Department of Veterinary Parasitology, University of Glasgow, Scotland, UK.
authors.forename: J B
authors.initials: JB
authors.lastname: McKeand
authors.forename: D P
authors.initials: DP
authors.lastname: Knox
authors.forename: J L
authors.initials: JL
authors.lastname: Duncan
authors.forename: M W
authors.initials: MW
authors.lastname: Kennedy
mesh_terms: D000110:Acetylcholinesterase / Q000276:immunology*; D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis / Q000235:genetics*; D004021:Dictyocaulus / Q000201:enzymology* / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D006168:Guinea Pigs; D007527:Isoenzymes / Q000276:immunology; D012635:Secretory Component / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000939:Epitopes; D007527:Isoenzymes; D012635:Secretory Component; D000110:Acetylcholinesterase