Exposure of CHO cells to AluI: comparison of chromosomal aberrations and cell survival.
Chinese hamster ovary (CHO) cells were exposed to various doses of the restriction endonuclease AluI in the presence of 2.2 M glycerol. Some of the cells were cultured for analysis of chromosomal aberrations and some for analysis of colony-forming ability. Cell killing is mainly mediated by chromosomal aberrations.
Obe
G
G
University GH Essen, Department of Genetics, Germany.
Eke
P
P
Johannes
C
C
D000818:Animals; D016466:CHO Cells / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D015252:Deoxyribonucleases, Type II Site-Specific / Q000633:toxicity*; D005990:Glycerol / Q000494:pharmacology; D009153:Mutagens / Q000633:toxicity*; D012044:Regression Analysis
D009153:Mutagens; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific; D005990:Glycerol