Translation in a wheat germ cell-free system of RNA from mitochondria of the normal and Texas male-sterile cytoplasms of maize (Zea mays L.).
RNA isolated from etiolated seedling shoot mitochondria of maize (Zea mays L.) with normal (N) or Texas male-sterile (T) cytoplasm stimulated the incorporation of [35S]-methionine into protein when added to a cell-free protein-synthesizing system from wheat germ. Discrete polypeptides with molecular masses of up to approximately 67 kDa were synthesized, and the pattern of bands was distinct from that obtained with total RNA. Products of translation of T-urf13 RNA were identified by immunoprecipitation, and of atpA, coxI, and coxII RNA by hybrid arrest of translation by the cloned gene. Several polypeptides were differentially synthesized from N and T mitochondrial RNA; these differences were more extensive than those found when isolated, intact, N and T mitochondria are allowed to synthesize proteins.
Hack
E
E
Department of Botany, Iowa State University, Ames 50011.
Hendrick
C A
CA
al-Janabi
S M
SM
Crane
V C
VC
Girton
L E
LE
D002474:Cell-Free System; D003593:Cytoplasm / Q000378:metabolism; D007246:Infertility / Q000235:genetics; D008928:Mitochondria / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics; D014176:Protein Biosynthesis; D012313:RNA / Q000235:genetics*; D000077278:RNA, Mitochondrial; D014908:Triticum; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism
D010940:Plant Proteins; D000077278:RNA, Mitochondrial; D012313:RNA