title: Phosphotyrosine-containing proteins are concentrated in differentiating cells during chicken embryonic development.
abstract: Protein tyrosine phosphorylation may be an important indicator of both the proliferative status and differentiation status of cells during embryonic development. To determine how each of these factors contributes to the level of phosphotyrosine-containing proteins detectable in embryonic tissues we have used immunohistochemistry with anti-phosphotyrosine antibodies on sections of developing chicken embryos. In contrast to an earlier study (Takata and Singer, 1988) we found proteins phosphorylated on tyrosine residues to be present in many different cells of the developing chicken embryo. The successful detection of phosphotyrosine-containing proteins in many cell types required the presence of sodium orthovanadate, a phosphotyrosine phosphatase inhibitor, during fixation. Despite the fact that the majority of tyrosine kinases identified to date are growth factor receptors, the highest levels of phosphotyrosine-containing proteins in many tissues were localized to populations of cells which were differentiating or migrating rather than dividing.
authors.affiliation: Department of Molecular and Cellular Growth Biology, Whittier Institute for Diabetes and Endocrinology, La Jolla, CA 92037.
authors.forename: G
authors.initials: G
authors.lastname: Patstone
authors.forename: P A
authors.initials: PA
authors.lastname: Maher
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002642:Chick Embryo; D007150:Immunohistochemistry; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D010750:Phosphoproteins; D019000:Phosphotyrosine; D014443:Tyrosine