title: Structural characterization of gelonin: evidence for separate antigenic and cytotoxic domains.
abstract: The N-terminal sequence of the three isoforms of gelonin is identical. Cyanogen bromide cleavage of gelonin produced fragments of Mr 17,000, 13,000, 11,000 and 7,000. The apparent Mr 17,000 component was identified as the N-terminal fragment and represents the major antigenic domain of the protein as it reacted with antibody to the native protein but this fragment did not inhibit protein synthesis in the in vitro translation assay. Our data may suggest possibilities for separation of antigenic and catalytic domains of this ribosome inactivating protein.
authors.affiliation: Reproduction Research Laboratory, Clinical Research Institute of Montreal, Quebec, Canada.
authors.forename: M R
authors.initials: MR
authors.lastname: Sairam
authors.forename: B R
authors.initials: BR
authors.lastname: Srinivasa
authors.forename: J
authors.initials: J
authors.lastname: Marcil
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016384:Consensus Sequence; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D015394:Molecular Structure; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology / Q000633:toxicity; D010940:Plant Proteins / Q000737:chemistry* / Q000276:immunology / Q000633:toxicity; D014176:Protein Biosynthesis / Q000187:drug effects; D011487:Protein Conformation; D011500:Protein Synthesis Inhibitors / Q000737:chemistry; D011817:Rabbits; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D000939:Epitopes; D010446:Peptide Fragments; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D054789:Ribosome Inactivating Proteins, Type 1; C025674:GEL protein, Gelonium multiflorum