title: Lymphocyte proliferation in response to immunodominant antigens of Brucella abortus 2308 and RB51 in strain 2308-infected cattle.
abstract: Lymphocyte proliferation in response to proteins from the Brucella abortus strain 2308 (S2308) and the lipopolysaccharide (LPS) O-antigen-deficient mutant of S2308, strain RB51 (SRB51), was measured in S2308-infected cattle following abortion. Supramammary and superficial cervical lymph node lymphocytes from infected cattle proliferated most when incubated with 27- to 18-kDa proteins of S2308 or SRB51. Proteins of SRB51, which contained no LPS O antigens, induced lymphocyte proliferation similar to that induced by S2308 proteins, which contained LPS O antigens. These results indicate that 27- to 18-kDa proteins, but not LPS O antigens, of S2308 and SRB51 are immunodominant in S2308-infected cattle as assessed by lymphocyte proliferation assays.
authors.affiliation: Brucellosis Research Unit, National Animal Disease Center, Ames, Iowa 50010.
authors.forename: M G
authors.initials: MG
authors.lastname: Stevens
authors.forename: S C
authors.initials: SC
authors.lastname: Olsen
authors.forename: N F
authors.initials: NF
authors.lastname: Cheville
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000302:isolation & purification; D002003:Brucella abortus / Q000276:immunology*; D002007:Brucellosis, Bovine / Q000276:immunology*; D002417:Cattle; D005260:Female; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial