title: Elevated calcium level induces calcium-dependent proteolysis of A-CAM (N-cadherin) in heart--analysis by detergent-treated model.
abstract: Calcium overload induces cardiac muscle cell dysfunction in cardiovascular diseases. We investigated the effects of elevated calcium level on adherens-junction-specific cell adhesion molecule (A-CAM). Incubation of Triton X-100-treated canine heart homogenate in the presence of Ca2+ reduced the content of A-CAM. Reduction in A-CAM requires milli-molar Ca2+ and was inhibited by protease inhibitors, leupeptin and calpeptin. Immunohistochemical observation revealed that m-calcium-activated neutral protease (m-CANP) was colocalized with A-CAM in intercalated disks. These data suggested that m-CANP proteolyzes A-CAM in response to calcium overload in cardiac muscle.
authors.affiliation: Department of Internal Medicine, Center for Adult Diseases, Osaka, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Fujio
authors.forename: F
authors.initials: F
authors.lastname: Yamada-Honda
authors.forename: H
authors.initials: H
authors.lastname: Funai
authors.forename: A
authors.initials: A
authors.lastname: Wada
authors.forename: S
authors.initials: S
authors.lastname: Kawashima
authors.forename: N
authors.initials: N
authors.lastname: Awata
authors.forename: N
authors.initials: N
authors.lastname: Shibata
mesh_terms: D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002118:Calcium / Q000502:physiology*; D002154:Calpain / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000378:metabolism; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D009206:Myocardium / Q000378:metabolism*; D017830:Octoxynol / Q000737:chemistry
chemical_list: D015820:Cadherins; D017830:Octoxynol; D002154:Calpain; D002118:Calcium