Role of lindane in membranes. Effects on membrane fluidity and activity of membrane-bound proteins.
The influence of lindane (gamma-hexachlorocyclohexane) on fluidity of plasma membranes from rat renal cortical tubules has been investigated. Preincubation with lindane increased membrane fluidity. This effect was accompanied by (i) a decrease in the transport of glucose with regard to the controls and (ii) an inhibition of the beta-adrenergic stimulatory activity upon cyclic AMP accumulation. However, a significant decrease of the membrane fluidity was found when rats were injected with lindane for 12 days. The injection of lindane exerted the opposite effect on the membrane proteins, the glucose transporter and the beta-adrenergic receptor, enhancing the glucose uptake and increasing the isoproterenol-stimulated cycle AMP accumulation. A possible explanation of the difference could involve a resistance to membrane disordering by lindane through a regulatory mechanism that would balance the activity of many lindane-sensitive proteins in insecticide-injected rats.
López-Aparicio
P
P
Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, Madrid, Spain.
Recio
M N
MN
Prieto
J C
JC
Pérez-Albarsanz
M A
MA
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects*; D000242:Cyclic AMP / Q000032:analysis; D005947:Glucose / Q000032:analysis; D001556:Hexachlorocyclohexane / Q000008:administration & dosage / Q000633:toxicity*; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D007684:Kidney Tubules / Q000187:drug effects; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects*; D008565:Membrane Proteins / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar
D008565:Membrane Proteins; D001556:Hexachlorocyclohexane; D000242:Cyclic AMP; D005947:Glucose; D007545:Isoproterenol