[Microspectrophotometric analysis of nuclear DNA contents related to the presence of alpha-fetoprotein in human hepatocellular carcinoma].
A total of 28 surgically resected hepatocellular carcinomas, including both fresh-smeared specimens and paraffin-embedded tissues, was stained with alpha-fetoprotein (AFP) antibody. In all cases we measured the DNA contents using microspectrophotometry. In 5 cases, analyses of the nuclear DNA histogram of both the AFP-positive and negative cells from the same specimen were successfully performed. The ratio of the S-G2.M phase of the AFP-positive cells was 2.24 times more than that of the AFP-negative cells. In addition, not only the ratio of S-G2.M phase cells, but also the incidence of aneuploidy pattern of AFP positive stained cases was higher than the negative stained cases. Histologically, the AFP-positive cases contained various differentiated tissues more than the negative cases. From these results, we confirmed that AFP production was related to the growth of HCC, and the results suggest that AFP producing HCC have a higher potential for growth than AFP non-producing HCC.
Kobayashi
K
K
First Dept. of Surgery, Kurume University School of Medicine.
Yamashita
Y
Y
Kamachi
M
M
Kurohiji
T
T
Horiuchi
H
H
Yanase
Y
Y
Kakegawa
T
T
D006528:Carcinoma, Hepatocellular / Q000737:chemistry* / Q000473:pathology; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry* / Q000473:pathology; D015591:Microspectrophotometry; D016612:Paraffin Embedding; D011003:Ploidies; D000509:alpha-Fetoproteins / Q000032:analysis*
D004273:DNA, Neoplasm; D000509:alpha-Fetoproteins