title: Role of glycoprotein gD in the adhesion of pseudorabies virus infected cells and subsequent cell-associated virus spread.
abstract: Pseudorabies virus (PrV) infected cells in suspension are able to adhere to a monolayer of uninfected cells by means of PrV glycoproteins expressed at the outer cell membrane, with gB and gC playing a major role as ligands and a heparinlike substance as receptor. In order to investigate the role of gD in this process and subsequent transmission of infectivity to contact cells, experiments with a gD deletion mutant, heparin and a monoclonal antibody (Mab) against gD were performed. The first indication that gD is active during cell adhesion was found by the observation that the binding of gD- PrV infected cells was five times weaker than that of wild type (WT) PrV infected cells. Further evidence was given by the use of a Mab against gD. Preincubation of WT PrV infected cells with this Mab led to a reduction of the percentage adhering cells from 69% to 49%. The same Mab inhibited the heparin independent and heparin resistant binding of WT PrV infected cells indicating that gD is important during both processes. Furthermore, it was demonstrated in a plaque assay that, after contact with a monolayer, gD- PrV infected cells in suspension were able to induce plaques with an efficiency of 1%. In conclusion, we can state that beside the interaction of the ligands gB and gC with a heparinlike receptor also the interaction of gD with a receptor which differs from a heparinlike substance mediates the binding of WT PrV infected cells to uninfected cells and that gD is not essential for the subsequent cell-to-cell spread of the virus.
authors.affiliation: Laboratory of Veterinary Virology, Faculty of Veterinary Medicine, University of Gent, Merelbeke, Belgium.
authors.forename: F P
authors.initials: FP
authors.lastname: Hanssens
authors.forename: H J
authors.initials: HJ
authors.lastname: Nauwynck
authors.forename: T C
authors.initials: TC
authors.lastname: Mettenlieter
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D006493:Heparin / Q000494:pharmacology; D011558:Herpesvirus 1, Suid / Q000254:growth & development* / Q000472:pathogenicity; D013552:Swine; D014759:Viral Envelope Proteins / Q000502:physiology*; D010948:Viral Plaque Assay
chemical_list: D014759:Viral Envelope Proteins; D006493:Heparin