Functional complementation of the radiation-sensitive mutant M10 cell line by human chromosome 5.
The mouse lymphoma (L5178Y) cell mutant M10 is defective in rejoining DNA double-strand breaks and is hypersensitive to ionizing radiation. The introduction of human chromosome 5 into M10 cells by microcell mediated chromosome transfer complemented the ionizing-radiation hypersensitivity defect of this cell line. The presence of chromosome 5 in the microcell hybrids was shown using PCR with chromosome-specific primers and fluorescence in situ hybridization. From this data we conclude that the gene that corrects the radiation hypersensitivity of M10 cells is located on chromosome 5 and tentatively assigned to the 5q14 to 5pter region. We designate this gene XRCC4L.
Stackhouse
M A
MA
Life Sciences Division, MS-M888, Los Alamos National Laboratory, NM 87545.
Ortiz
J B
JB
Sato
K
K
Chen
D J
DJ
D000818:Animals; D002895:Chromosomes, Human, Pair 5; D004260:DNA Repair / Q000235:genetics; D005816:Genetic Complementation Test; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D009154:Mutation; D016133:Polymerase Chain Reaction; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured