Mast cells in experimental allergic encephalomyelitis: characterization, distribution in the CNS and in vitro activation by myelin basic protein and neuropeptides.
Mast cells (MC) have been implicated in the pathogenesis of experimental allergic encephalomyelitis (EAE). In order to further evaluate their role, several morphological and functional studies were performed. Semiquantitative counts of histological sections showed a significant reduction in MC numbers in EAE brains. In addition, a higher proportion of EAE MC (about 50-70%) appeared degranulated compared with about 20% degranulation in controls. Central nervous system (CNS) MC exhibited staining properties of connective tissue MC and about 98% of them, both in diseased and control rats, were located in the thalamus. They were not present in the spinal cord and did not relate to EAE lesions. In vitro incubation of peritoneal MC (of connective tissue phenotype) with either MBP, or with neuropeptides such as substance P or bradykinin resulted in release of beta-hexosaminidase and histamine. The latter responses were similar in both EAE and control rats. It is suggested that the decrease in number and in granular content of CNS MC in EAE may reflect prior in vivo activation. The fact that MC were activated by MBP and by neuropeptides in vitro suggests a possible mechanism of MC activation in EAE.
Brenner
T
T
Department of Neurology, Hadassah University Hospital, Jerusalem, Israel.
Soffer
D
D
Shalit
M
M
Levi-Schaffer
F
F
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002490:Central Nervous System / Q000473:pathology*; D003594:Cytoplasmic Granules / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000473:pathology*; D005260:Female; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D004676:Myelin Basic Protein / Q000494:pharmacology*; D015213:Neuroimmunomodulation; D009479:Neuropeptides / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D011917:Rats, Inbred Lew; D013373:Substance P / Q000494:pharmacology; D013788:Thalamus / Q000473:pathology; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
D004676:Myelin Basic Protein; D009479:Neuropeptides; D013373:Substance P; D001619:beta-N-Acetylhexosaminidases; D001920:Bradykinin