Differences in the response to mutagens between two V79 sublines.
Two V79 Chinese hamster sublines (V79-UL and V79-MZ) which differed markedly with respect to their spontaneous pattern of mutations at the HPRT locus were comparatively investigated in genotoxicity tests with ethyl methanesulphonate (EMS). EMS-induced frequencies of HPRT mutations and sister chromatid exchanges (SCEs) were much higher in V79-MZ than in V79-UL. V79-MZ were not hypersensitive against EMS and had normal frequencies of spontaneous gene mutations and chromosome aberrations. Baseline SCE frequencies at various BrdUrd concentrations were slightly increased compared to V79-UL. EMS induced a similar amount of chromosome aberrations in both cell lines but exchange figures occurred with lower frequency in V79-MZ. The results indicate that specific and significant differences in the response to mutagens may exist between 'normal' Chinese hamster cell lines which might be relevant for genotoxicity testing.
Speit
G
G
Abteilung Klinische Genetik, Universität Ulm, Germany.
Habermeier
B
B
Helbig
R
R
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000494:pharmacology*; D012854:Sister Chromatid Exchange
D009153:Mutagens; D005020:Ethyl Methanesulfonate; D007041:Hypoxanthine Phosphoribosyltransferase