title: Influence of portal branch ligation on the outcome of repeat dearterializations of an experimental liver tumor in the rat.
abstract: It has been suggested that the portal vein should be occluded during intermittent hepatic dearterialization in order to induce a more complete ischemia of the tumor. In this experiment the influence of portal branch ligation in combination with repeat dearterializations on a liver tumor was investigated. Twenty-seven rats were randomly allocated to sham treatment (n = 6); portal branch ligation (PBL) (n = 7); 120 min of repeat dearterialization (n = 7); and portal branch ligation (PBL) in combination with 50 min of repeat dearterialization (n = 7) (once a day during 5 days). The results showed that portal branch ligation alone did not alter the tumor growth compared with sham treatment (P > 0.05), nor did portal branch ligation in combination with repeat dearterializations for 50 min (P > 0.05). However, tumor growth delay was achieved following 120 min of repeat dearterializations without occlusion of the portal branch (P < 0.01 versus all the other groups). There was a significant weight loss of the lobe undergoing PBL, whether dearterialization was added or not (P < 0.001). The liver nucleotide/DNA and RNA/DNA ratio significantly decreased as well. Histological examination showed that > 50% of tumor cells became necrotic after repeat dearterializations for 2 hr indicating a significant damage to tumor tissue. In contrast, PBL in combination with repeat dearterializations for 50 min induced extensive liver necrosis without having any influence on tumor growth.
authors.affiliation: Department of Surgery, Lund University, Sweden.
authors.forename: L Q
authors.initials: LQ
authors.lastname: Wang
authors.forename: B G
authors.initials: BG
authors.lastname: Persson
authors.forename: U
authors.initials: U
authors.lastname: Stenram
authors.forename: S
authors.initials: S
authors.lastname: Bengmark
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D006499:Hepatic Artery / Q000601:surgery*; D008026:Ligation; D008114:Liver Neoplasms, Experimental / Q000098:blood supply* / Q000473:pathology; D008297:Male; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D011169:Portal Vein / Q000601:surgery*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar