Enhanced expression of LFA-3 on human T-cell lines and leukemic cells carrying human T-cell-leukemia virus type 1.
Previously, we studied the surface expression of LFA-1 (CD11a/CD18) and ICAM-1 (CD54) in a panel of 16 human T-cell lines and found that those carrying HTLV-1 pro-viruses expressed high levels of ICAM-1. Enhanced expression of ICAM-1 was also seen in fresh leukemic cells from ATL patients. In the present study, we systematically examined the surface expression of 19 different cell-adhesion molecules (CAMs) (the immunoglobulin superfamily, the homing receptors, the integrins, etc.) in a panel of 20 human T-cell lines (6 HTLV-1-negative lines and 14HTLV-1-positive lines). The surface expression of LFA-3 (CD58) was constantly enhanced in HTLV-1-positive T-cell lines except for MT-1, an ATL-derived cell line, which was devoid of a number of CAMs including ICAM-1. LFA-3 proteins and mRNA were found at strongly increased levels in HTLV-1-positive T-cell lines.
Imai
T
T
Shionogi Institute for Medical Science, Osaka, Japan.
Tanaka
Y
Y
Fukudome
K
K
Takagi
S
S
Araki
K
K
Yoshie
O
O
D015703:Antigens, CD / Q000032:analysis* / Q000235:genetics; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D015152:Blotting, Northern; D018801:CD2 Antigens; D018106:CD28 Antigens / Q000032:analysis; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules / Q000032:analysis*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D006801:Humans; D018960:Hyaluronan Receptors; D015151:Immunoblotting; D016023:Integrins / Q000032:analysis; D015458:Leukemia, T-Cell / Q000276:immunology*; D051917:Leukosialin; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D011971:Receptors, Immunologic / Q000032:analysis; D016201:Receptors, Lymphocyte Homing / Q000032:analysis; D012795:Sialoglycoproteins / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D018106:CD28 Antigens; D018968:CD58 Antigens; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D016023:Integrins; D051917:Leukosialin; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D016201:Receptors, Lymphocyte Homing; C465014:SPN protein, human; D012795:Sialoglycoproteins