Autoantibodies against a novel DNA-binding protein: DNA-protein interaction as a requisite for expression of antigenic reactivity.
A novel DNA-binding protein complex, the HB complex, has been characterized by means of autoantibodies. Three proteins of 9000, 7500, and 7000 Da constitute the HB complex. The 9000- and 7500-Da proteins are phosphorylated. Autoantibodies recognize the 7000-Da protein when it is bound to DNA. No reactivity against any protein was observed when the complex was dissociated from DNA. The three proteins are acidic (pI 5-6.2), and the complex was able to bind to synthetic double-stranded DNAs of different composition.
Juarez
C
C
Department of Immunology, Hospital de Sant Pau, Universitat Autonoma de Barcelona, Spain.
Vila
J L
JL
Agusti
M
M
Gelpi
C
C
Amengual
M J
MJ
Cardona
S
S
Rodriguez-Sanchez
J L
JL
D000818:Animals; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000276:immunology*; D002417:Cattle; D005591:Chemical Fractionation; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000276:immunology* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans
D001323:Autoantibodies; D004268:DNA-Binding Proteins; D000939:Epitopes; D004247:DNA