title: Fas ligand mediates activation-induced cell death in human T lymphocytes.
abstract: A significant proportion of previously activated human T cells undergo apoptosis when triggered through the CD3/T cell receptor complex, a process termed activation-induced cell death (AICD). Ligation of Fas on activated T cells by either Fas antibodies or recombinant human Fas-ligand (Fas-L) also results in cytolysis. We demonstrate that these two pathways of apoptosis are causally related. Stimulation of previously activated T cells resulted in the expression of Fas-L mRNA and lysis of Fas-positive target cells. Fas-L antagonists inhibited AICD of T cell clones and staphylococcus enterotoxin B (SEB)-specific T cell lines. The data indicate AICD in previously stimulated T cells is mediated by Fas/Fas-L interactions.
authors.affiliation: Immunex Research and Developmental Corporation, Seattle, Washington 98101.
authors.forename: M R
authors.initials: MR
authors.lastname: Alderson
authors.forename: T W
authors.initials: TW
authors.lastname: Tough
authors.forename: T
authors.initials: T
authors.lastname: Davis-Smith
authors.forename: S
authors.initials: S
authors.lastname: Braddy
authors.forename: B
authors.initials: B
authors.lastname: Falk
authors.forename: K A
authors.initials: KA
authors.lastname: Schooley
authors.forename: R G
authors.initials: RG
authors.lastname: Goodwin
authors.forename: C A
authors.initials: CA
authors.lastname: Smith
authors.forename: F
authors.initials: F
authors.lastname: Ramsdell
authors.forename: D H
authors.initials: DH
authors.lastname: Lynch
mesh_terms: D000954:Antigens, Surface / Q000502:physiology*; D017209:Apoptosis; D002478:Cells, Cultured; D053222:Fas Ligand Protein; D015870:Gene Expression; D006801:Humans; D066298:In Vitro Techniques; D015759:Ionomycin / Q000494:pharmacology; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000502:physiology*; D018613:Microscopy, Confocal; D012333:RNA, Messenger / Q000235:genetics; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D019014:fas Receptor
chemical_list: D000954:Antigens, Surface; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D019014:fas Receptor; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate