Artificial protein vaccines with predetermined tertiary structure: application to anti-HIV-1 vaccine design.
A successful approach to the development of a safe and effective synthetic vaccine requires that different B and T cell epitopes of the infectious agent be included in the vaccine construction. In this paper we suggest a new approach to vaccine design in the form of an artificial protein with a predetermined tertiary structure (PTS vaccines). Based on B and T cell epitope properties, we substantiate the possible use for vaccine construction of one well-known protein spatial motif--the four-alpha-helix bundle. Antigenic determinants of cellular immunity (amphipathic alpha-helices) and humoral immunity (flexible hydrophilic loop regions) are used as blocks for vaccine design. General principles of PTS vaccine construction have been applied to anti-HIV-1 vaccine design.
Eroshkin
A M
AM
Research Institute of Molecular Biology, NPO VECTOR, Koltsovo, Novosibirsk Region, Russia.
Zhilkin
P A
PA
Shamin
V V
VV
Korolev
S
S
Fedorov
B B
BB
D016915:AIDS Vaccines / Q000737:chemistry*; D015195:Drug Design; D000939:Epitopes / Q000737:chemistry*; D015686:Gene Products, env / Q000737:chemistry; D015683:Gene Products, gag / Q000737:chemistry; D005813:Genes, Synthetic; D015488:HIV Antigens / Q000737:chemistry*; D015497:HIV-1 / Q000276:immunology*; D008958:Models, Molecular; D008962:Models, Theoretical; D015202:Protein Engineering; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic / Q000737:chemistry*
D016915:AIDS Vaccines; D000939:Epitopes; D015686:Gene Products, env; D015683:Gene Products, gag; D015488:HIV Antigens; D011506:Proteins; D011994:Recombinant Proteins; C085784:TBI protein, recombinant; D014614:Vaccines, Synthetic