Effects of dexamethasone in a model of lung hyperresponsiveness in the rat.
In rats, Sephadex treatment on days 0, 2 and either 4 or 5 resulted in a blood and lung eosinophilia, an increase in lung cell fragility, an increase in the functional activity of peritoneal eosinophils in vitro and a sustained increased responsiveness of lung parenchymal strips to KCl, 5-hydroxytryptamine (5-HT) and carbachol that was not associated with oedema or gross fibrosis. The corticosteroid dexamethasone, when given before each injection of Sephadex, reduced all these effects of Sephadex. When given 30 min after the last injection of Sephadex, dexamethasone had no effect on the number of blood and lung eosinophils but it did reduce the functional activity of peritoneal eosinophils, the increased lung cell fragility and the hyperresponsiveness to 5-HT. Repeated administration of dexamethasone to rats with an established hyperresponsiveness that was no longer associated with cellular inflammation had minimal effects on this hyperresponsiveness.
Piercy
V
V
SmithKline Beecham Pharmaceuticals, Epsom, Surrey, UK.
Arch
J R
JR
Baker
R C
RC
Cook
R M
RM
Hatt
P A
PA
Spicer
B A
BA
D000818:Animals; D016535:Bronchial Hyperreactivity / Q000188:drug therapy*; D003601:Cytotoxicity Tests, Immunologic; D003907:Dexamethasone / Q000494:pharmacology*; D003911:Dextrans / Q000494:pharmacology; D004804:Eosinophils / Q000187:drug effects; D006909:Hydroxyproline / Q000494:pharmacology; D007958:Leukocyte Count / Q000187:drug effects; D008163:Luminescent Measurements; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008297:Male; D010084:Oxidation-Reduction; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012397:Rosette Formation
D003911:Dextrans; D003907:Dexamethasone; C025614:sephadex; D006909:Hydroxyproline