title: Identification of an aprotinin antiviral domain.
abstract: Digestion of the proteinase inhibitor aprotinin, by clostripain, a cysteine proteinase, yielded five oligopeptide fragments. Two fragments exhibited both antiviral and antibacterial activities, two fragments only antiviral activity, and one fragment showed no antimicrobial activity. One of the former oligopeptides showed antiviral activity against human herpes simplex virus type 1 and bovine parainfluenza virus type 3. It consisted of the hexapeptide Y-F-Y-N-A-K corresponding to amino acids 21-26 of intact aprotinin. An identical synthetic peptide had the same antiviral spectrum as the natural hexapeptide, exhibited no antibacterial activity, and was also devoid of trypsin inhibiting activity. Intact aprotinin, in contrast, is ineffective against human herpes simplex virus 1 and bovine parainfluenza virus 3 but possesses antibacterial properties against several bacterial species [(1992) J. Appl. Bact. 72, 180-187].
authors.affiliation: Institute of Veterinary Physiology, University of Zürich, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Pellegrini
authors.forename: U
authors.initials: U
authors.lastname: Thomas
authors.forename: M
authors.initials: M
authors.lastname: Franchini
authors.forename: M
authors.initials: M
authors.lastname: Stöckli
authors.forename: S
authors.initials: S
authors.lastname: Klauser
authors.forename: P
authors.initials: P
authors.lastname: Hunziker
authors.forename: R
authors.initials: R
authors.lastname: von Fellenberg
mesh_terms: D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000737:chemistry* / Q000494:pharmacology; D007611:Aprotinin / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D003546:Cysteine Endopeptidases / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000187:drug effects; D008969:Molecular Sequence Data; D010224:Parainfluenza Virus 3, Human / Q000187:drug effects; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D010446:Peptide Fragments; D014361:Trypsin Inhibitors; D007611:Aprotinin; D003546:Cysteine Endopeptidases; C019913:clostripain