title: Superoxide anion-induced histamine release from rat peritoneal mast cells.
abstract: We investigated the properties of superoxide anion (O2-)-induced histamine release from rat peritoneal mast cells using superoxide anion radicals obtained from potassium superoxide (KO2). KO2 elicited a rapid histamine release in a dose-dependent fashion, without lactate dehydrogenase (LDH) release. The KO2-induced release was temperature- and energy-dependent. KO2 rapidly increased the intracellular free Ca2+ concentration, accompanied by a marked increase of Ca(2+)-uptake. These findings indicate that the increase in intracellular Ca2+ concentration is involved in the initiation of KO2-induced histamine release, and KO2 could be used as an agent of O2(-)-induced biological reactions.
authors.affiliation: Department of Pharmacology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Akagi
authors.forename: Y
authors.initials: Y
authors.lastname: Katakuse
authors.forename: N
authors.initials: N
authors.lastname: Fukuishi
authors.forename: T
authors.initials: T
authors.lastname: Kan
authors.forename: R
authors.initials: R
authors.lastname: Akagi
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000494:pharmacology*
chemical_list: D013481:Superoxides; D007770:L-Lactate Dehydrogenase; D002118:Calcium