Infiltrating gamma delta T-cells and selectin endothelial ligands in the cutaneous phytohaemagglutinin-induced inflammatory reaction.
The 24 h phytohaemagglutinin-induced skin inflammatory site (intradermal and subcutaneous) was studied in inbred MHC-homozygous (SLAb/b) pigs and it was found, by immunohistology, that the predominant lymphocytes in the infiltrate are CD2-CD4-CD8-sIg-T-cells, the Null/gamma delta T-cell family, identified using the monoclonal antibodies (mAbs) MAC320 and MAC319 (which recognises a subset of MAC320+ cells). A large percentage of the infiltrating cells expressed the gamma delta T-cell receptor phenotype identified by binding of the mAb 86D. Fewer CD2+, CD8+ and CD4+ cells were present and surface immunoglobulin positive (sIg+) cells were virtually absent in the infiltrate. Areas of lymphocytic infiltration were associated with endothelial activation as determined by expression of the E-selectin and a ligand for the L-selectin.
Whyte
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A
Department of Immunology, AFRC Babraham Institute, Cambridge, UK.
Haskard
D O
DO
Binns
R M
RM
D000818:Animals; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002634:Chemotaxis, Leukocyte; D017449:Dermatitis, Allergic Contact / Q000276:immunology*; D019040:E-Selectin; D004727:Endothelium / Q000276:immunology; D019041:L-Selectin; D008024:Ligands; D008562:Membrane Glycoproteins / Q000276:immunology; D010835:Phytohemagglutinins; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D011971:Receptors, Immunologic / Q000276:immunology; D016201:Receptors, Lymphocyte Homing / Q000276:immunology; D013552:Swine; D013601:T-Lymphocytes
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D008024:Ligands; D008562:Membrane Glycoproteins; D010835:Phytohemagglutinins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011971:Receptors, Immunologic; D016201:Receptors, Lymphocyte Homing; C028784:phytohemagglutinin-P; D019041:L-Selectin