Distinct effects of clinically used anthracycline antibiotics on ras oncogene-expressed cells.
Doxorubicin, pirarubicin, and FAD-104, but not aclarubicin or MX 2, flattened the morphology of NIH3T3 cells that had been transformed by human H-ras and K-ras. The effect appeared on almost all cells, as early as 2 d following exposure to the antibiotics at concentrations inhibiting cell growth by 50% or more. The morphological alteration accompanied other normal cell phenotypes, such as the restoration of actin stress fibers, anchorage dependence of cell growth and an increase in nucleoside diphosphate (NDP) kinase activity. NIH3T3 cells transformed by src and other tumor cell lines responded less prominently, if at all.
Tsuchiya
K S
KS
Showa College of Pharmaceutical Sciences, Tokyo, Japan.
Kanbe
T
T
Hori
M
M
Uehara
Y
Y
Takahashi
Y
Y
Takeuchi
T
T
D016475:3T3 Cells; D000199:Actins / Q000187:drug effects; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002461:Cell Line, Transformed; D004317:Doxorubicin / Q000031:analogs & derivatives / Q000494:pharmacology; D004789:Enzyme Activation; D015870:Gene Expression; D011905:Genes, ras; D006801:Humans; D051379:Mice; D009701:Nucleoside-Diphosphate Kinase / Q000378:metabolism; D013194:Staining and Labeling
D000199:Actins; D000903:Antibiotics, Antineoplastic; C059081:ME 2303; D004317:Doxorubicin; C027260:pirarubicin; D009701:Nucleoside-Diphosphate Kinase