Activation of the alternative pathway of complement by apoptotic Jurkat cells.
Jurkat T cells die of apoptosis upon exposure to anti-Fas mAb. Here we show that although the alternative complement pathway generally does not attack homologous cells, anti-Fas-induced apoptotic Jurkat T cells were attacked antibody-independently by the alternative pathway of human complement and opsonized with iC3b, which is a ligand of the complement receptor type 3 (CR3) of phagocytes. These results suggest that apoptotic cells become the targets of the homologous alternative complement pathway, which facilitates the clearance of apoptotic cells by phagocytes.
Matsui
H
H
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Tsuji
S
S
Nishimura
H
H
Nagasawa
S
S
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology; D017209:Apoptosis; D015153:Blotting, Western; D002460:Cell Line; D003179:Complement C3b / Q000378:metabolism; D003170:Complement Pathway, Alternative; D006801:Humans; D011951:Receptors, Complement / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D019014:fas Receptor
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D011951:Receptors, Complement; D019014:fas Receptor; D003179:Complement C3b