Antihypertensive effects of the endothelin receptor antagonist BQ-123 in conscious spontaneously hypertensive rats.
Until recently, a role for endothelin-1 (ET-1) in the development and/or maintenance of hypertension has been based solely on indirect findings, e.g., elevated circulating levels of peptide. However, with the development of specific ET-1 receptor antagonists it is now possible to examine this relationship directly. The present study describes the hemodynamic effects of systemic infusions of BQ-123, a selective endothelin (ETA)-receptor antagonist, in conscious, freely moving spontaneously hypertensive (SHRs) and normotensive Wistar-Kyoto (WKY) rats. Sustained infusions of BQ-123 (0.16-164 nmol/kg/min i.v. for 6 h) produced dose-dependent reductions in mean arterial pressure (approximately 30 mm Hg) in SHRs that were long-lasting (> 18 h) and reversible. Whereas cardiac output remained unaltered during BQ-123 infusion, the observed reduction in blood pressure was accompanied by a significant decrease (16%) in total peripheral resistance, indicating that the fall in blood pressure (an effect that was independent of the vehicle used and was not observed in WKY rats) was related primarily to peripheral vasodilation. Thus, the present study provides direct evidence showing that ET-1-receptor antagonists are effective antihypertensive (rather than hypotensive) agents and that endogenous ET-1 is involved in the pathophysiology of hypertension.
Ohlstein
E H
EH
Department of Cardiovascular Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939.
Douglas
S A
SA
Ezekiel
M
M
Gellai
M
M
D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects; D002302:Cardiac Output / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D065128:Endothelin Receptor Antagonists; D006439:Hemodynamics / Q000187:drug effects; D008297:Male; D010456:Peptides, Cyclic / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D014655:Vascular Resistance / Q000187:drug effects
D000959:Antihypertensive Agents; D065128:Endothelin Receptor Antagonists; D010456:Peptides, Cyclic; C072247:cyclo(Trp-Asp-Pro-Val-Leu)