Modulation of integrins expression during human osteoblasts "in vitro" differentiation.
We here report the modulation of the adhesion of cultured human osteoblasts on Laminin during the acquisition of differentiated phenotype. We also show that interference with the differentiation program caused by treatment with Retinoic acid of the cultures, causes changes of the capability to adhere to Laminin and type I Collagen. The younger or dedifferentiated cells have lower capability to bind to Laminin or Collagen. The maturation associated changes are specific for the adhesion to the above substrata and do not involve the adhesion to FN or plastic. The alpha subunit(s) of the integrin receptor(s) for these proteins is likely to be responsible for the modulation adhesion to Laminin and Collagen.
Manduca
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P
Istituto di Fisiologia, Università di Genova.
Pistone
M
M
Sanguineti
C
C
Lu
K
K
Stringa
E
E
D002448:Cell Adhesion; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen; D005109:Extracellular Matrix / Q000378:metabolism; D005353:Fibronectins; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D039201:Integrin alpha3beta1; D039564:Integrin alphaV; D016023:Integrins / Q000096:biosynthesis* / Q000235:genetics; D007797:Laminin; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D038981:Receptors, Collagen; D017469:Receptors, Fibronectin / Q000096:biosynthesis / Q000235:genetics; D017474:Receptors, Laminin / Q000096:biosynthesis / Q000235:genetics; D014212:Tretinoin / Q000494:pharmacology
D005353:Fibronectins; D039201:Integrin alpha3beta1; D039564:Integrin alphaV; D016023:Integrins; D007797:Laminin; D038981:Receptors, Collagen; D017469:Receptors, Fibronectin; D017474:Receptors, Laminin; D014212:Tretinoin; D003094:Collagen