title: Spatiotemporal expression of SCIP and myelin genes in rat brain during early postnatal development.
abstract: The expression of the SCIP and myelin genes (PLP, BP and MAG) was quantitated by Northern blot analysis during early postnatal rat brain development. The SCIP gene was already profoundly upregulated on day 1 postpartum. The SCIP message level in the forebrain was 4- and 17-fold higher than the message level in the cerebellum and brainstem, respectively. By day 20, the SCIP gene expression declined in the forebrain to approximately 5% of the day-1 level, and became undetectable in the brainstem and cerebellum. The myelin gene expression sharply upregulated at approximately day 7-8, and in general, was highest in the brainstem, and lowest in the forebrain. The results demonstrate that the expression of the SCIP gene in the oligodendrocytic lineage cells is relatively low, whereas other cellular elements feature very active expression of the gene during the early postnatal development. Additionally, the variation in the relative ratios of the myelin messages among the brain regions indicates that the levels of expression of the myelin genes are not tightly synchronized.
authors.affiliation: Department of Anatomy, West Virginia University School of Medicine, Morgantown 26506-9128.
authors.forename: P R
authors.initials: PR
authors.lastname: Yassini
authors.forename: R C
authors.initials: RC
authors.lastname: Wiggins
authors.forename: G W
authors.initials: GW
authors.lastname: Konat
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000254:growth & development*; D001923:Brain Chemistry / Q000235:genetics*; D015342:DNA Probes; D005260:Female; D015870:Gene Expression / Q000502:physiology*; D009186:Myelin Sheath / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011247:Pregnancy; D012313:RNA / Q000032:analysis / Q000302:isolation & purification; D051381:Rats
chemical_list: D015342:DNA Probes; D012313:RNA