Bio-anticlastogenic effects of unsaturated fatty acids included in fish oil--docosahexaenoic acid, docosapentaenoic acid, and eicosapentaenoic acid--in cultured Chinese hamster cells.
Bio-anticlastogenic effects of unsaturated fatty acids--cis-4,7,10,13,16,19-docosahexaenoic acid (DHA), cis-7,10,13,16,19-docosapentaenoic acid (DPA), and cis-5,8,11,14,17-eicosapentaenoic acid (EPA)--on chemically induced chromosome aberrations were studied in cultured Chinese hamster cells. The induction of chromosome aberrations by the crosslinking agents mitomycin C (MMC) and cisplatin (DDP), the SN-1 type alkylating agents N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG), methyl nitrosourea (MNU), and ethyl nitrosourea (ENU), and the SN-2 type alkylating agent ethyl methanesulfonate (EMS), but not by the SN-1 type alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and the SN-2 type alkylating agent methyl methanesulfonate (MMS), was suppressed by post-treatment with DHA, DPA, and EPA. Since there was no opportunity to inactivate mutagens by desmutagenic mechanisms under the post-treatment schedule used, the results demonstrate the bio-anticlastogenicity of unsaturated fatty acids. Suppression by the unsaturated fatty acids was observed when cells were treated during the G2 phase, suggesting that G2 events were responsible for the bio-anticlastogenic effects. Two saturated fatty acids with the same number of carbons as the studied unsaturated fatty acids--docosanoic acid and eicosanoic acid--did not affect chromosome aberration induction, suggesting the necessity of unsaturation for fatty acid bio-anticlastogenicity.
Sasaki
Y F
YF
Laboratory of Genetics, Faculty of Chemical and Biological Engineering, Hachinohe National College of Technology, Aomori, Japan.
Sakaguchi
M
M
Yamagishi
T
T
Yamada
H
H
Shirasu
Y
Y
D000477:Alkylating Agents / Q000633:toxicity; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D016466:CHO Cells; D016009:Chi-Square Distribution; D002869:Chromosome Aberrations; D006224:Cricetinae; D003432:Cross-Linking Reagents / Q000633:toxicity; D004260:DNA Repair; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D005395:Fish Oils / Q000494:pharmacology*; D016195:G2 Phase; D016296:Mutagenesis / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity; D013489:Suppression, Genetic
D000477:Alkylating Agents; D016587:Antimutagenic Agents; D003432:Cross-Linking Reagents; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D009153:Mutagens; D004281:Docosahexaenoic Acids; D015118:Eicosapentaenoic Acid; C026219:docosapentaenoic acid