Clustering of monoclonal antibodies recognizing different members of the WC1 gene family.
Mouse L cell lines expressing two different bovine WC1 glycoproteins were produced by transfection of the cells with the corresponding cDNAs. The cell lines were used to analyze the reactivities of 67 monoclonal antibodies (mAbs) which recognize bovine gamma/delta T cells. The results indicated that preliminary clustering of mAbs can be achieved based on their recognition of epitopes expressed on all gene products, or of epitopes encoded by individual members of the gene family. The studies also showed that at least three members of the WC1 gene family are expressed, although it is not yet known how many can be expressed by individual bovine gamma/delta T cells. Final clustering of the WC1 mAbs will not be possible until the exact number of expressed gene products is known, and the reactivities of the mAbs with these products have been analyzed.
MacHugh
N D
ND
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Wijngaard
P L
PL
Clevers
H C
HC
Davis
W C
WC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002417:Cattle / Q000276:immunology*; D002460:Cell Line; D004247:DNA / Q000235:genetics; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry / Q000662:veterinary; D015870:Gene Expression; D007739:L Cells; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D005810:Multigene Family; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection
D000911:Antibodies, Monoclonal; D000939:Epitopes; D008562:Membrane Glycoproteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D004247:DNA