title: BDNF and NT-3 induce intracellular Ca2+ elevation in hippocampal neurones.
abstract: The effects of neurotrophins on intracellular Ca2+ levels in rat hippocampal neurones were studied in vitro using fura-2 fluorescence microscopy. BDNF and NT-3, but not NGF, rapidly increased cytoplasmic Ca2+ concentrations in these neurones ten-fold to reach 1 microM. Moreover in some of the neurones both BDNF and NT-3 elicited Ca2+ responses, indicative of the presence of functional receptors for these neurotrophins in the same cell. In these cultures approximately 80% of the hippocampal neurones were stained with antibodies against full-length TrkB. The expression of functional TrkB was also confirmed by RNA analysis. These results demonstrate the presence of functional receptors for BDNF and NT-3 in hippocampal neurones.
authors.affiliation: Department of Neurochemistry, Max Planck Institute for Psychiatry, Martinsried, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Berninger
authors.forename: D E
authors.initials: DE
authors.lastname: García
authors.forename: N
authors.initials: N
authors.lastname: Inagaki
authors.forename: C
authors.initials: C
authors.lastname: Hahnel
authors.forename: D
authors.initials: D
authors.lastname: Lindholm
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009473:Neuronal Plasticity / Q000187:drug effects; D009474:Neurons / Q000378:metabolism*; D020933:Neurotrophin 3; D011247:Pregnancy; D012313:RNA / Q000378:metabolism; D051381:Rats; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000187:drug effects / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3; D017475:Receptors, Nerve Growth Factor; D011994:Recombinant Proteins; D012313:RNA; D020794:Receptor Protein-Tyrosine Kinases; D002118:Calcium