Novel soluble, high-affinity gastrin-releasing peptide binding proteins in Swiss 3T3 fibroblasts.
Swiss 3T3 cells contained substantial amounts of soluble and specific [125I]GRP binders. Like the membrane-associated GRP receptor, they were of high affinity, saturable, bound to GRP(14-27) affinity gels, and exhibited specificity for GRP(14-27) binding. They differed in that acid or freezing destroyed specific binding, specific binding exhibited different time and temperature effects, no detergent was required for their solubilization, ammonium sulfate fractionation yielded different profiles, the M(rs) were lower, GRP(1-16) also blocked binding, and a polyclonal anti-GRP receptor antiserum did not bind on Western blots. The isolated, soluble GRP binding protein(s) rapidly degraded [125I]GRP. These soluble GRP binding proteins may play a role in the regulation of the mitogenic effects of GRP on these cells.
Kane
M A
MA
Medical Oncology Section, Denver Veterans Affairs Medical Center, CO 80220.
Portanova
L B
LB
Kelley
K
K
Holley
M
M
Ross
S E
SE
Boose
D
D
Escobedo-Morse
A
A
Alvarado
B
B
D016475:3T3 Cells; D000818:Animals; D015153:Blotting, Western; D001839:Bombesin / Q000031:analogs & derivatives; D002352:Carrier Proteins / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000378:metabolism; D019886:Gastrin-Releasing Peptide; D051379:Mice; D010455:Peptides / Q000378:metabolism*; D011485:Protein Binding; D018004:Receptors, Bombesin / Q000302:isolation & purification / Q000378:metabolism; D012995:Solubility; D013373:Substance P / Q000378:metabolism
D002352:Carrier Proteins; D010455:Peptides; D018004:Receptors, Bombesin; D013373:Substance P; D019886:Gastrin-Releasing Peptide; D010450:Endopeptidases; D001839:Bombesin