Increase of phosphotyrosine levels in mouse urine and liver during liver regeneration after partial hepatectomy.
To determine how cell proliferation contributes to phosphotyrosine (P-Tyr) level in body we investigated the changes of P-Tyr levels in mouse urine and liver during liver regeneration after partial hepatectomy (PH). After PH, the P-Tyr contents in urine and liver increased approximately 14- and 1.7-fold, respectively, at 2-4 days, and P-Tyr phosphatase activity in liver decreased to 50% at 2 days. These levels returned to the control level at 10 days. After orthovanadate administration, P-Tyr level increased as P-Tyr phosphatase activity decreased. These results suggest that P-Tyr level in liver is reflected in urinary P-Tyr level during liver regeneration, and P-Tyr phosphatase is important in the regulation of P-Tyr level.
Kataoka
H
H
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Nakai
K
K
Makita
M
M
D000818:Animals; D006498:Hepatectomy; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D019000:Phosphotyrosine; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D012016:Reference Values; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000652:urine; D014638:Vanadates / Q000494:pharmacology
D019000:Phosphotyrosine; D014638:Vanadates; D014443:Tyrosine; D017027:Protein Tyrosine Phosphatases