Interleukin-2-dependent T-cell lines established from paroxysmal nocturnal hemoglobinuria patients.
Peripheral blood T lymphocytes obtained from two patients with paroxysmal nocturnal hemoglobinuria (PNH) were immortalized with human T-lymphotropic virus type 1 (HTLV-1). These cells showed interleukin-2 (IL-2)-dependent cell growth in culture. Cell surface analysis showed that they had the phenotype of a helper/inducer T subset that was positive for CD2, CD3, and CD4, but negative for CD8, similar to adult T-cell leukemia cells induced by HTLV-1. These cell lines lacked glycosylphosphatidylinositol (GPI)-anchored proteins, CDw52, CD55 (decay-accelerating factor; DAF), and CD59 on the cell surface, whereas intracellular DAF protein was detected. These T-subset cell lines with a PNH phenotype did not synthesize GPI anchor, whereas a control cell line, similarly prepared from the T cells of a healthy volunteer, produced the anchor. The control cells expressed CDw52, DAF, and CD59 on the cell surface and showed the phenotype of a helper/inducer subset. Southern blot analysis confirmed the clonality of each cell line. These CD4+ T-cell lines with a PNH phenotype and a subset-matched control counterpart could be a useful model for PNH investigation.
Nakakuma
H
H
Second Department of Internal Medicine, Kumamoto University School of Medicine, Japan.
Nagakura
S
S
Horikawa
K
K
Hidaka
M
M
Kawaguchi
T
T
Iwamoto
N
N
Sanada
I
I
Kagimoto
T
T
Takatsuki
K
K
D015703:Antigens, CD / Q000032:analysis; D018958:CD55 Antigens; D002460:Cell Line; D017261:Glycosylphosphatidylinositols / Q000096:biosynthesis; D006457:Hemoglobinuria, Paroxysmal / Q000276:immunology*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D007621:Karyotyping; D008213:Lymphocyte Activation / Q000187:drug effects; D008562:Membrane Glycoproteins / Q000032:analysis; D013601:T-Lymphocytes / Q000502:physiology*
D015703:Antigens, CD; D018958:CD55 Antigens; D017261:Glycosylphosphatidylinositols; D007376:Interleukin-2; D008562:Membrane Glycoproteins