Production of multiple cytokines by cultured human melanomas.
We screened a panel of 8 primary and 21 metastatic melanoma cell lines for constitutive secretion of cytokines. Melanomas expressed bioactivity for TGF-beta (8/25 lines) and IFN (7/12), but not IL-2. Immunoassays detected IL-1 alpha (4/25), IL-1 beta (12/25), IL-6 (13/29), IL-8 (29/29), TGF-beta 2 (5/12) and GM-CSF (11/29), but not IL-3, IL-4, TNF-alpha, or IFN-gamma. There was no preferential association of cytokine production with cells cultured from primary versus metastatic disease, and only IL-8 was produced by all lines tested. These data demonstrate that cultured melanomas produce a variety of cytokines which are potentially capable of influencing tumor growth in vivo.
Bennicelli
J L
JL
Cancer Center University of Pennsylvania School of Medicine, Philadelphia.
Guerry
D
D
D001681:Biological Assay; D017077:Culture Media, Conditioned / Q000737:chemistry; D016207:Cytokines / Q000096:biosynthesis* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007372:Interferons / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis; D008545:Melanoma / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D016207:Cytokines; D016209:Interleukin-8; D007378:Interleukins; D009363:Neoplasm Proteins; D016212:Transforming Growth Factor beta; D007372:Interferons