Ribosomal RNA gene (rrn) organization in enterococci.
A cloned 1.8-kb probe containing the 3' end of 16S ribosomal RNA and the 5' end of 23S ribosomal RNA from Enterococcus hirae was used to analyze various endonuclease digests of enterococci. In the ATCC strains tested we observed a remarkable conservation of the ApaI sites in the rrn operons, and a partial conservation of EcoRI sites. Using a number of other endonuclease digestions with the ApaI rrn probe, we estimate the number of rrn operons in enterococci to be between five and six.
Sechi
L A
LA
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.
Zuccon
F M
FM
Mortensen
J E
JE
Daneo-Moore
L
L
D004269:DNA, Bacterial; D016983:Enterococcus / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012339:RNA, Ribosomal, 28S / Q000235:genetics*; D015183:Restriction Mapping
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012339:RNA, Ribosomal, 28S