[An immunohistochemical study of retinoblastoma].
Various immunohistochemical marders were detected in 27 specimens of human retinoblastoma by the avidin-biotin-peroxidase complex technique, using mono- and polyclonal antibodies. The glial markers, glial fibrillary acidic protein and Leu-7 were detected in the retinal tissue component and reactive perivascular glial cells in the tumor mass. In only 4 of the 27 specimens, Leu-7 was positive in the glial cells randomly interspersed among the tumor cells and not associated with blood vessels. The neuronal marker, neuron-specific enolase, stained strongly positive in undifferentiated tumor cells in most (21/27) of the specimens. Rhodopsin, the photoreceptor marker which exists only in the outer segments of photoreceptor cells, was detected in the fleurettes of 1 tumor and in the rosettes of 6 tumors, these results support the view that retinoblastoma is chiefly neuronal in nature with tendency to differentiate into photoreceptor cells and rarely into glial cells.
Sun
X L
XL
Beijing Institute of Ophthalmology.
Zheng
B H
BH
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis*; D018959:CD57 Antigens; D005134:Eye Neoplasms / Q000737:chemistry*; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D012175:Retinoblastoma / Q000737:chemistry*; D012243:Rhodopsin / Q000032:analysis
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D014408:Biomarkers, Tumor; D018959:CD57 Antigens; D005904:Glial Fibrillary Acidic Protein; D012243:Rhodopsin; D010751:Phosphopyruvate Hydratase