title: Superparamagnetic beads: applications of solid-phase RT-PCR.
abstract: Solid-phase RT-PCR applications with Dynabeads Oligo (dT)25 allow the reproducible isolation and detection of low-abundance cDNA sequences from small cell and tissue samples. The reusable properties of the beads reduce reagent cost and provide large amounts of target cDNAs for downstream applications. Magnetic separation of pure target mRNA/cDNA sequences eliminates labor-intensive precipitation and extraction steps and provides mRNA/cDNA sequences absent of contamination.
authors.affiliation: Dynal, Inc., Lake Success, NY 11042.
authors.forename: S
authors.initials: S
authors.lastname: Augenstein
mesh_terms: D018076:DNA, Complementary / Q000302:isolation & purification; D015723:Gene Library; D008280:Magnetics; D008863:Microspheres; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase; D001613:beta 2-Microglobulin / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D001613:beta 2-Microglobulin; D012194:RNA-Directed DNA Polymerase