Promoter of the mouse gene encoding calcium-independent nitric oxide synthase confers inducibility by interferon-gamma and bacterial lipopolysaccharide.
We have proved that iNOS is regulated by induction of transcription. The induction of iNOS mRNA requires synthesis of an intermediary protein(s) and probably involves action of a protein kinase or kinases. We have cloned and sequenced a 1.7-kilobase pair fragment from the 5'-flanking region of the iNOS gene and proved that this region contains a promoter which confers inducibility of iNOS by LPS and IFN-gamma.
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Department of Medicine, Cornell University Medical College, New York, NY.
Nathan
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D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000235:genetics; D004790:Enzyme Induction / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D019001:Nitric Oxide Synthase; D011401:Promoter Regions, Genetic / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007371:Interferon-gamma; D004247:DNA; D003513:Cycloheximide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D002118:Calcium