[MBP P89-101 induced experimental allergic encephalomyelitis in mice and establishment of CD4+ T cell line specific to MBP P89-101].
SJL/J mice were immunized with peptide 89-101 of myelin basic protein in FCA and developed EAE around 13th day after immunization. Lymph-nodes were collected at the time of onset and lymphocytes were prepared and cultured with MBP P89-101 in vitro for 3 weeks. X-ray irradiated (3000R) syngeneic spleen cells were added as antigen presenting cells. Antigen stimulated blast cells were isolated with Ficoll-Hypaque. MBP P89-101 specificity of cells were measured by 3H-TdR incorporation. MBP P89-101 activated cells were stained with PE-CD4 and analyzed by FACS. The results showed that all of the blast cells were of CD4 positive phenotype, specific to MBP P89-101.
Lin
J
J
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing.
Owens
T
T
D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002460:Cell Line; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000473:pathology*; D005260:Female; D008198:Lymph Nodes / Q000473:pathology; D051379:Mice; D004676:Myelin Basic Protein / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology
D004676:Myelin Basic Protein; D010446:Peptide Fragments