[Fluctuation of HCV quasi-species population during interferon therapy; analysis by single strand conformation polymorphism].
We investigated the fluctuation of hepatitis C virus (HCV) quasispecies during interferon therapy by single strand conformation polymorphism (SSCP) analysis. In 13 of 16 interferon ineffective patients, the predominant HCV population was replaced with other quasispecies during the treatment. Especially, in 9 patients, a part of the HCV quasispecies, pre-existing before interferon therapy, became predominant after the therapy. These results indicate that sensitivity to interferon differs among HCV quasispecies and that interferon selects resistant HCV strains. Existence of such HCV quasispecies seems to be associated with interferon treatment failure.
Enomoto
N
N
Second Department of Internal Medicine, Tokyo Medical and Dental University.
Kurosaki
M
M
Koizumi
K
K
Asahina
Y
Y
Sakuma
I
I
Murakami
T
T
Yamamoto
C
C
Marumo
F
F
Sato
C
C
D001483:Base Sequence; D002908:Chronic Disease; D016174:Hepacivirus / Q000145:classification / Q000235:genetics*; D006526:Hepatitis C / Q000382:microbiology* / Q000628:therapy; D006801:Humans; D007372:Interferons / Q000627:therapeutic use*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012367:RNA, Viral / Q000032:analysis
D012367:RNA, Viral; D007372:Interferons