A novel strategy of c-myc oncogene in NK activity regulation not related to the W6/32 MHC class-I epitope.
The c-myc gene encodes a nuclear protein whose precise function is still not fully understood. Introduction of a c-myc gene into a number of cell lines leads to an increase in their susceptibility to NK-cell lysis. It was reported earlier that c-myc can induce a decrease in the membrane expression of the MHC class-I molecules and this may be one of the factors that render target cells relatively more susceptible to NK lysis. In this contribution, we show, in a human LCL line transfected with a constitutively active c-myc gene, an increased sensitivity to NK lysis, which correlates with an augmented effector-target binding ability of c-myc-transfected LCLs and with a high ICAM-I expression rather than with down-regulation of MHC class-I W6/32 epitope expression.
La Cava
A
A
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli Federico II, Italy.
Carbone
E
E
Moscarella
A
A
Barcová
M
M
Salzano
S
S
Zappacosta
S
S
Fontana
S
S
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000502:physiology; D015152:Blotting, Northern; D002144:Callithrix; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000502:physiology; D002472:Cell Transformation, Viral; D003080:Cold Temperature; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000502:physiology*; D015870:Gene Expression; D016259:Genes, myc / Q000502:physiology*; D004854:Herpesvirus 4, Human; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000502:physiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007694:Killer Cells, Natural / Q000276:immunology / Q000502:physiology*; D008223:Lymphoma / Q000235:genetics / Q000276:immunology; D008722:Methods; D051379:Mice; D010641:Phenotype; D014162:Transfection
D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D018799:Intercellular Adhesion Molecule-1