Cloning and immunological characterisation of Echinococcus granulosus ferritin.
A cDNA clone of Echinococcus granulosus has been isolated from an expression library screened with sera from cystic echinococcosis patients. The deduced amino acid sequence shows 56% homology to the heavy chain of human ferritin. E. granulosus ferritin contains 173 amino acid residues and has a calculated molecular weight of 19830 Da and a statistical isoelectric point of 7.6. Functionally important amino acid residues of the ferroxidase centre are conserved in comparison with other ferritins. In vitro-translated E. granulosus ferritin was tested for its diagnostic potential by immunoprecipitation. The antigenic reactivity exhibited a good potential for the further development of E. granulosus ferritin as an immunodiagnostic tool for human hydatidosis.
Ersfeld
K
K
Department of Biological Sciences, University of Salford, UK.
Craig
P S
PS
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D003001:Cloning, Molecular; D003429:Cross Reactions; D018076:DNA, Complementary / Q000032:analysis; D018509:DNA, Helminth / Q000032:analysis / Q000235:genetics; D004285:Dogs; D004443:Echinococcosis / Q000276:immunology*; D004446:Echinococcus / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D005293:Ferritins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D012550:Schistosoma mansoni / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D011993:Recombinant Fusion Proteins; D005293:Ferritins