Anti-T11.1 and -T11.2 monoclonal antibodies play a different role in CD2-mediated signal transduction.
We comparatively evaluated (Ca2+)i mobilization after triggering with a stimulatory pair of CD2 (CD2.9, anti-T11.1 + CD2.1, anti-T11.2) or CD3 mAbs in the differentiated T-cell line Jurkat, using INDO-1 labeling and cytofluorimetry. The results obtained showed different (Ca2+)i mobilization kinetics following CD2 or CD3 stimulation (the former being slower than the latter), not due to different association kinetics of mAbs. In a nonreciprocal manner, however, preliminary interaction with CD2.1 (anti-T11.2) followed by CD2.9 (anti-T11.1) induces a rapid (Ca2+)i rise, similar to CD3 stimulation, as shown by preincubation experiments. There is no interference between CD2.9 and CD2.1 mAb binding. CD2.1 mAb by itself is unable to induce (Ca2+)i mobilization; in addition, preincubation with CD2.1 mAb did not modify the CD2, CD3, CD45, or CD28 immunoprecipitation patterns. Triggering of the epitope recognized by CD2.1 mAb may favor, possibly via conformational changes of CD2 molecule or (Ca2+)i-unrelated metabolic effect(s), optimal signal transduction.
Bagnasco
M
M
Center for Oncology and Experimental Therapy, INSERM U119, Marseille, France.
Franco
M D
MD
Lopez
M
M
Nunes
J
J
Lipcey
C
C
Mawas
C
C
Salamero
J
J
Olive
D
D
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology* / Q000502:physiology*; D001667:Binding, Competitive; D018801:CD2 Antigens; D017252:CD3 Complex / Q000276:immunology; D002118:Calcium / Q000502:physiology*; D002454:Cell Differentiation; D000939:Epitopes; D006801:Humans; D011233:Precipitin Tests; D011971:Receptors, Immunologic / Q000276:immunology* / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D013601:T-Lymphocytes / Q000502:physiology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D017252:CD3 Complex; D000939:Epitopes; D011971:Receptors, Immunologic; D002118:Calcium