title: Protein ubiquitination in the posterior silk glands of Bombyx mori.
abstract: Ubiquitin gene expression and the ubiquitination of proteins in the posterior silk glands (PSG) of B. mori were analyzed developmentally with respect to fibroin synthesis and degeneration. Two ubiquitin transcripts are expressed throughout larval stages, and the level of each transcript is regulated differently. The larger transcript, a polyubiquitin mRNA, was abundant during the molt stage, while levels of the smaller ubiquitin transcript increased immediately after molting. Only a single 65 kDa ubiquitinated protein was detected late in the 5th instar, and the amount increased up to spinning stage. This ubiquitinated protein may participate in the PSG degeneration.
authors.affiliation: Division of Biology, National Institute of Radiological Sciences, Chiba-shi, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ichimura
authors.forename: K
authors.initials: K
authors.lastname: Mita
authors.forename: M
authors.initials: M
authors.lastname: Numata
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D012831:Bombyx / Q000378:metabolism*; D015870:Gene Expression; D019476:Insect Proteins; D007814:Larva; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D014176:Protein Biosynthesis; D011499:Protein Processing, Post-Translational; D012313:RNA / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D012333:RNA, Messenger / Q000096:biosynthesis; D012627:Sebaceous Glands / Q000378:metabolism; D047011:Silk; D014158:Transcription, Genetic; D014452:Ubiquitins / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D019476:Insect Proteins; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D047011:Silk; D014452:Ubiquitins; D012313:RNA