title: The catalytic properties of the reverse transcriptase of the lentivirus equine infectious anemia virus.
abstract: The reverse transcriptase (RT) of equine infectious anemia virus (EIAV) shares sequence similarity with the RTs of other lentiviruses, particularly with the RTs of human immunodeficiency viruses types 1 and 2 (HIV-1 and HIV-2, respectively), the causative agents of acquired immunodeficiency syndrome (AIDS). There is a 41-42% sequence identity between EIAV RT and both HIV RTs (which have 61% sequence identity to each other). We have compared the enzymic properties of EIAV RT with those of HIV-1 RT. Several aspects of the activities of EIAV RT differ from the corresponding activities of HIV-1 RT. There are significant differences in the inhibition of the DNA polymerase activities by the deoxynucleoside triphosphate analogs, 3'-azido-2,3'-dideoxythymidine triphosphate, dideoxyTTP and dideoxyGTP and by the nonnucleoside inhibitor, tetrahydroimidazo[4,5,1-jk-1,4]benzodiazepin-2-(1H)-one and thione; in the dependence of DNA polymerase and RNase H activities on pH; in the inhibition of the DNA polymerase activities by the thiol-specific reagent N-ethylmaleimide; in the specific DNA polymerase activity; in the inhibition of the ribonuclease H activity by the zinc chelator orthophenanthroline. However, there are several cases in which EIAV RT and HIV-1 RT are more similar than was previously found for HIV-1 RT and HIV-2 RT. These include the Km values for the DNA polymerase activities, the heat stability of the DNA polymerase functions and the specific activity of the RNase H function.
authors.affiliation: Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Israel.
authors.forename: T
authors.initials: T
authors.lastname: Rubinek
authors.forename: S
authors.initials: S
authors.lastname: Loya
authors.forename: M
authors.initials: M
authors.lastname: Shaharabany
authors.forename: S H
authors.initials: SH
authors.lastname: Hughes
authors.forename: P K
authors.initials: PK
authors.lastname: Clark
authors.forename: A
authors.initials: A
authors.lastname: Hizi
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D001569:Benzodiazepines / Q000494:pharmacology; D002384:Catalysis; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004795:Enzyme Stability; D005033:Ethylmaleimide / Q000494:pharmacology; D054303:HIV Reverse Transcriptase; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000494:pharmacology; D004860:Infectious Anemia Virus, Equine / Q000201:enzymology*; D010618:Phenanthrolines / Q000494:pharmacology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D018894:Reverse Transcriptase Inhibitors; D016914:Ribonuclease H / Q000378:metabolism
chemical_list: D000998:Antiviral Agents; D007093:Imidazoles; D010618:Phenanthrolines; D011994:Recombinant Proteins; D018894:Reverse Transcriptase Inhibitors; D001569:Benzodiazepines; C067413:R-82913; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase; D016914:Ribonuclease H; D005033:Ethylmaleimide; C025205:1,10-phenanthroline