Staining of human telomeres with primed in situ labeling (PRINS).
As described, the PRINS method is a very rapid and reliable way of staining human telomeres. To obtain the maximum frequency of stained telomeres, the primer (CCCTAA)7 should be used, although the average frequency never quite reaches 100%. The frequency is strongly dependent on the age of the individual, being significantly higher in children and newborns than in adults. A difference between the (CCCTAA)7 primer and the complementary primer is demonstrated and a possible explanation is proposed, namely, that gaps in the C-rich strand cause chain elongation termination after the addition of only one dTTP molecule.
Therkelsen
A J
AJ
Institute of Human Genetics, Aarhus University, Denmark.
Nielsen
A
A
Koch
J
J
Hindkjaer
J
J
Kølvraa
S
S
D000328:Adult; D000818:Animals; D001483:Base Sequence; D002871:Chromosome Banding; D017931:DNA Primers; D005260:Female; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D013194:Staining and Labeling; D016615:Telomere / Q000648:ultrastructure*; D013768:Tetrahymena
D017931:DNA Primers