Crystallization and preliminary X-ray diffraction studies of human RANTES.
The chemotactic cytokine RANTES (Regulated on Activation, Normal T-cell Expressed and Secreted) is a potent chemoattractant and activator of a number of leukocytes, with a molecular mass of 8 kDa. Crystals of this protein have been grown from 100 mM sodium acetate buffer (pH 4.6) containing 200 mM magnesium acetate, with 20% (w/v) PEG 4000 and 6% (v/v) glycerol. The crystals grow as thick rods, which diffract to at least 1.8 A resolution on a rotating anode X-ray source. The crystals belong to space group p2(1)2(1)2(1) with unit cell dimensions a = 95.14 A, b = 57.58 A and c = 24.01 A with alpha = beta = gamma = 90 degrees. The asymmetric unit contains two molecules of the RANTES monomer, with a VM of 2.0 A(3)/Da.
Shaw
J P
JP
Rosentiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA 02254.
Kryger
G
G
Cleasby
A
A
Wonacott
A
A
Power
C A
CA
Proudfoot
A E
AE
Wells
T N
TN
D018946:Chemokine CCL5; D006801:Humans; D008222:Lymphokines / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry; D014961:X-Ray Diffraction
D018946:Chemokine CCL5; D008222:Lymphokines; D011994:Recombinant Proteins