Antisense src expression inhibits U937 human leukemia cell proliferation in conjunction with reduction of c-myb expression.
To elucidate the role of pp60c-src in U937 human monoblastoid leukemia cell proliferation, recombinant plasmids containing the src gene or myb gene, which could produce antisense src or antisense myb RNA after dexamethasone treatment, were constructed and transfected into U937 cells (U937-ASRC, U937-AMYB). pp60c-src synthesis in U937-ASRC was diminished by the third day after induction of antisense src RNA and the cell proliferation was reduced, furthermore, the amount of p75c-myb was significantly decreased by the third day. p75c-myb synthesis in U937-AMYB was diminished by the second day after induction of antisense myb RNA and the cell growth was significantly inhibited but the amount of pp60c-src in U937-AMYB was not reduced. These results suggest that a decrease in the amount of pp60c-src leads to an inhibition of p75c-myb expression and subsequent reduction in the U937 cell proliferation.
Waki
M
M
First Department of Internal Medicine, Kagawa Medical School, Japan.
Kitanaka
A
A
Kamano
H
H
Tanaka
T
T
Kubota
Y
Y
Ohnishi
H
H
Takahara
J
J
Irino
S
S
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D003907:Dexamethasone / Q000494:pharmacology; D016391:Genes, src; D006801:Humans; D007700:Kinetics; D007938:Leukemia; D016403:Lymphoma, Large B-Cell, Diffuse; D009857:Oncogenes; D010957:Plasmids; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D020598:Proto-Oncogene Proteins c-myb; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000096:biosynthesis / Q000378:metabolism*; D016372:RNA, Antisense / Q000378:metabolism*; D015183:Restriction Mapping; D013997:Time Factors; D014162:Transfection; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D016372:RNA, Antisense; D003907:Dexamethasone; D016392:Proto-Oncogene Proteins pp60(c-src)