Growth hormone-binding protein in partially hepatectomized rats.
The role of the liver in regulating serum growth hormone-binding protein (GH-BP) was studied. We measured rat serum GH-BP and insulin-like growth factor 1 (IGF-1) 30 min to 96 h after 70% partial hepatectomy (PHP) or sham operation in adult male rats. Serum GH-BP declined sharply from 5.8 +/- 0.1% at baseline to 3.9 +/- 0.5% by 48 h following PHP. By 72 h serum GH-BP at baseline to 3.9 +/- 0.5% by 48 h following PHP. By 72 h serum GH-BP returned to baseline level and remained at that level 96 h postoperatively. In sham-operated female rats, serum GH-BP was about 2-fold higher than in males (10.5 +/- 1.46 versus 5.8 +/- 0.2%), whereas 24 h after hepatectomy a significant drop of about 50% was observed (p < 0.001). Serum IGF-1 decreased within 2-4 h postoperatively in both sham-operated and PHP groups, but thereafter was lower in the PHP rats, up to 48 h after operation, compared to sham-operated rats (p < 0.03). The study shows that the liver has an important role in the determination of serum GH-BP levels. The return to normal GH-BP level, even before the liver regained its full size following hepatectomy, suggests an increase in GH-BP production by the regenerating liver.
Baruch
Y
Y
Department of Medicine B, Rambam Medical Center, Haifa, Israel.
Amit
T
T
Hertz
P
P
Enat
R
R
Shoshany
G
G
Youdim
M B
MB
Hochberg
Z
Z
D000818:Animals; D002352:Carrier Proteins / Q000097:blood* / Q000378:metabolism; D005260:Female; D006498:Hepatectomy; D018970:Insulin-Like Growth Factor Binding Protein 1; D008099:Liver / Q000378:metabolism*; D008103:Liver Cirrhosis / Q000601:surgery; D008115:Liver Regeneration; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D002352:Carrier Proteins; D018970:Insulin-Like Growth Factor Binding Protein 1; C051815:somatotropin-binding protein