title: Evolution of prothrombin: isolation and characterization of the cDNAs encoding chicken and hagfish prothrombin.
abstract: The cDNA sequences of chicken and hagfish prothrombin have been determined. The sequences predict that prothrombin from both species is synthesized as a prepro-protein consisting of a putative Gla domain, two kringle domains, and a two-chain protease domain. Chicken and hagfish prothrombin share 51.6% amino acid sequence identity (313/627 residues). Both chicken and hagfish prothrombin are structurally very similar to human, bovine, rat, and mouse prothrombin and all six species share 41% amino acid sequence identity. Amino acid sequence alignments of human, bovine, rat, mouse, chicken, and hagfish prothrombin suggest that the thrombin B-chain and the propeptide-Gla domain are the regions most constrained for the common function(s) of vertebrate prothrombins.
authors.affiliation: Department of Biochemistry, University of British Columbia, Vancouver, Canada.
authors.forename: D K
authors.initials: DK
authors.lastname: Banfield
authors.forename: D M
authors.initials: DM
authors.lastname: Irwin
authors.forename: D A
authors.initials: DA
authors.lastname: Walz
authors.forename: R T
authors.initials: RT
authors.lastname: MacGillivray
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D015723:Gene Library; D006196:Hagfishes / Q000235:genetics*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D017433:Protein Structure, Secondary; D011516:Prothrombin / Q000235:genetics* / Q000302:isolation & purification; D012313:RNA / Q000032:analysis; D015183:Restriction Mapping; D016415:Sequence Alignment; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D010446:Peptide Fragments; D012313:RNA; D011516:Prothrombin