Lipopolysaccharides of Campylobacter jejuni serotype O:19: structures of O antigen chains from the serostrain and two bacterial isolates from patients with the Guillain-Barré syndrome.
An O antigenic polysaccharide was liberated from the lipopolysaccharide of high M(r) from Campylobacter jejuni serotype O:19 by acetic acid hydrolysis of the ketosidic linkage to lipid A. The structure of the polysaccharide was established in several one- and two-dimensional 1H and 13C NMR experiments, fast atom bombardment mass spectrometry and methylation linkage analysis of the permethylated glycan and its degradation products. It is concluded that the glycan is a derivative of hyaluronic acid in which the beta-D-glucuronic acid residues in the alternating sequence [-4)-beta-D-GlcA-(1-->3)-beta-D-GlcNAc-(1]n are present as amides of 2-amino-2-deoxyglycerol. Parallel experiments were performed on O antigens liberated from lipopolysaccharides of high M(r) from bacterial isolates that had been obtained from two patients who subsequently developed the Guillain-Barré syndrome. Within the limits of structural analysis by NMR spectroscopy and methylation linkage analysis, both these O antigens were identical to that from the serostrain.
Aspinall
G O
GO
Department of Chemistry, York University, North York, Toronto, Ontario, Canada.
McDonald
A G
AG
Pang
H
H
D016123:Campylobacter jejuni / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011129:Polyradiculoneuropathy / Q000382:microbiology*; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D016339:Spectrometry, Mass, Fast Atom Bombardment
D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial