The identification of the pterin-binding domain in the nitric oxide synthase's sequence.
Comparative studies of primary sequences of nitric oxide synthase (NOS) and pterin-dependent enzymes has shown that the biopterin-dependent site of NOS is localized between the regions responsible for calmodulin and FMN binding. On the example of NOS from rat brain it was demonstrated that this biopterin-binding site is located in the region 750-769. Pairwise alignment of amino acids sequences of NOS from rat brain and rat xantine dehydrogenase revealed that the two proteins are 39.7% identical.
Uvarov VYu
Institute of biomedical chemistry, Moscow, Russia.
Lyashenko
A A
AA
D000594:Amino Acid Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology; D016384:Consensus Sequence; D007256:Information Systems; D008969:Molecular Sequence Data; D019001:Nitric Oxide Synthase; D011622:Pterins / Q000378:metabolism*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014968:Xanthine Dehydrogenase / Q000737:chemistry
D011622:Pterins; D019001:Nitric Oxide Synthase; D014968:Xanthine Dehydrogenase; D000594:Amino Acid Oxidoreductases