title: Antigenic specificity of antibodies from patients with linear basement membrane deposition of IgA.
abstract: We reviewed the immunoreactivity of sera binding to the epidermal side of basement membrane split skin from 13 adults and 8 children with IgA alone, 9 adults with IgA and IgG and 7 adults with IgA and ocular pemphigoid. Immunoblots were done against previously described 45-, 97-, 180- and 230-kD antigens, and reactivity was confirmed by elution of antibody from nitrocellulose and binding to the basement membrane. Ten of 13 adults and 7 of 8 children reacted with the 97-kD antigen. Sera with both IgA and IgG reacted in varying patterns and on occasion with more than 1 antigen. All 7 patients with ocular cicatricial pemphigoid reacted uniquely with a 45-kD antigen.
authors.affiliation: Department of Internal Medicine, Veterans Affairs Medical Center, Salt Lake City, Utah.
authors.forename: J J
authors.initials: JJ
authors.lastname: Zone
authors.forename: E
authors.initials: E
authors.lastname: Pazderka Smith
authors.forename: D
authors.initials: D
authors.lastname: Powell
authors.forename: T B
authors.initials: TB
authors.lastname: Taylor
authors.forename: J B
authors.initials: JB
authors.lastname: Smith
authors.forename: L J
authors.initials: LJ
authors.lastname: Meyer
mesh_terms: D000328:Adult; D001485:Basement Membrane / Q000276:immunology; D015153:Blotting, Western; D002648:Child; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis / Q000276:immunology*; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D010390:Pemphigoid, Benign Mucous Membrane / Q000276:immunology; D012867:Skin / Q000276:immunology*; D012872:Skin Diseases, Vesiculobullous / Q000276:immunology*
chemical_list: D000939:Epitopes; D007070:Immunoglobulin A; D007074:Immunoglobulin G