IgE and IgG cross-reactivity among Lol p I and Lol p II/III. Identification of the C-termini of Lol p I, II, and III as cross-reactive structures.
In this study, the homologous C-termini of Lol p I, Lol p II, and Lol p III were shown to contain cross-reactive B-cell epitopes. This was demonstrated by inhibition studies with purified Lol p I, II, and III and synthetic peptides of their C-termini. It was ruled out that the observed cross-reactivity was caused by cross-contamination of the purified allergens. Both human IgE and IgG bound to the C-terminus of Lol p I. These antibodies were cross-reactive with Lol p II and, more specifically, with its C-terminus. Within a small panel of allergic patients, no cross-reactivity with Lol p III was found. A hyperimmune polyclonal rabbit antiserum against Lol p I also recognized the Lol p I C-terminus. As for human antibodies, cross-reactivity with Lol p II and its C-terminus was demonstrated. Cross-reactivity with Lol p III was demonstrated with C-terminal peptides, but not with native Lol p III. A polyclonal rabbit antiserum against Lol p II bound to the C-terminal peptides of both Lol p II and III. This binding was inhibited with Lol p I, confirming that cross-reactive structures exist not only on the C-termini of Lol p II and Lol p I, but also of Lol p III and Lol p I. The existence of cross-reactivity between Lol p I and Lol p II and III possibly contributes to the frequently observed cosensitization for these allergens in grass-pollen-allergic patients.
van Ree
R
R
Central Laboratory, The Netherlands Red Cross Blood Transfusion Service, University of Amsterdam, The Netherlands.
van Leeuwen
W A
WA
van den Berg
M
M
Weller
H H
HH
Aalberse
R C
RC
D000485:Allergens / Q000276:immunology*; D000818:Animals; D001667:Binding, Competitive; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D066298:In Vitro Techniques; D008129:Lolium; D010446:Peptide Fragments / Q000276:immunology; D011058:Pollen / Q000276:immunology*; D011817:Rabbits; D011852:Radioallergosorbent Test; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology
D000485:Allergens; D000939:Epitopes; D007074:Immunoglobulin G; D010446:Peptide Fragments; D007073:Immunoglobulin E