Stimulation of protein-tyrosine phosphorylation in gerbil hippocampus after global forebrain ischemia.
Tyrosine phosphorylation in the gerbil hippocampus after a transient ischemia was analyzed by immunoblotting and immunohistochemistry. In control hippocampus, the phosphotyrosine was detected in many proteins of 165 to 10 kDa and the immunostain showed a distinct distribution. The ischemic insult induced various alterations of the phosphotyrosine immunoreactivities in both ischemia-resistant and -vulnerable neurons which were associated with alterations in the expression of 165 to 19 kDa-immunoreactive bands. These results suggest that tyrosine phosphorylation is involved in the ischemic hippocampus to play a role in the development of early and delayed neuronal deaths in CA4 and CA1 neurons, respectively.
Yokota
M
M
Department of Neurosurgery, Hyogo College of Medicine, Japan.
Saido
T C
TC
Miyaji
K
K
Tani
E
E
Kawashima
S
S
Suzuki
K
K
D000818:Animals; D005260:Female; D005849:Gerbillinae; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D015151:Immunoblotting; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D009928:Organ Specificity; D010750:Phosphoproteins / Q000032:analysis / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine; D017966:Pyramidal Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D012016:Reference Values; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D010750:Phosphoproteins; D019000:Phosphotyrosine; D014443:Tyrosine