title: Radiation-induced mitotic gene conversion frequency in yeast is modulated by the conditions allowing DNA double-strand break repair.
abstract: Repair of DNA double-strand breaks (DSB) involves recombinational processes which may lead to gene conversion (intragenic recombination). Using the diploid yeast mutant rad54-3 heteroallelic for his1 (his1-7/his1-1) and temperature conditional for DSB rejoining, radiation induced gene conversion was investigated as dependent on DSB repair under different postirradiation conditions. Gene conversion is negligible under conditions preventing DSB repair (36 degrees C). In contrast, gene conversion is observed when cells are incubated at the permissive temperature (23 degrees C) both under growth and nongrowth conditions. However, there is a much higher yield of convertants for cells incubated under growth as opposed to nongrowth conditions. These results can most plausibly be explained by the cell cycle regulated enhancement of the expression of genes such as PMS and POL3 known to be involved in gene conversion processes and/or the enhanced recombination in transcriptionally active genes. 'Nutrient stress' inducible responses and/or cell cycle specific recombination pathways leading to gene conversion events preferentially in S-phase cells seem to be less likely.
authors.affiliation: Abteilung Klinische Strahlenbiologie und Klinische Strahlenphysik, Universität Göttingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Frankenberg-Schwager
authors.forename: D
authors.initials: D
authors.lastname: Frankenberg
authors.forename: R
authors.initials: R
authors.lastname: Harbich
mesh_terms: D002470:Cell Survival / Q000528:radiation effects; D003470:Culture Media; D004260:DNA Repair / Q000235:genetics*; D005785:Gene Conversion / Q000528:radiation effects*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal / Q000235:genetics*; D008938:Mitosis; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D011836:Radiation Tolerance; D013696:Temperature
chemical_list: D003470:Culture Media; D009692:Nucleic Acid Heteroduplexes