title: Analysis of epitopic residues introduced into the hybrid peptide vaccines prepared according to the cassette theory.
abstract: In our previous study, we prepared a synthetic peptide vaccine (46F/HA127-133/54A) against influenza strain A/Aichi/2/68 (H3N2) virus by introducing haemagglutinin (HA) 127-133 to an I-Ab,b binding component that consisted of residues 43-46 and 54-58 of an I-Ab,d binding peptide, 46F50V54A. This hybrid peptide vaccine induced considerable immunological responses against A/Aichi/2/68 as well as against the peptide vaccine in I-Ab mice. In the present study, we have attempted to increase the immunogenicity of the peptide vaccine by introducing HA peptides of various lengths into the I-Ab,d binding components consisting of residues 43-46 and 54-58 or 43-47 and 53-58 of 46F50V54A. We demonstrate here that, among the peptide vaccines prepared, 46F/HA127-133/54A (18 mer) consisting of HA127-133 and the I-Ab,d binding component constructed from 43-47 and 53-58 of 46F50V54A induces the most vigorous T-cell responses and neutralizing antibodies against A/Aichi/2/68 in both I-Ab and I-Ad mice.
authors.affiliation: Section of Pathology, School of Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Naruse
authors.forename: K
authors.initials: K
authors.lastname: Ogasawara
authors.forename: K
authors.initials: K
authors.lastname: Takami
authors.forename: K
authors.initials: K
authors.lastname: Kajino
authors.forename: T
authors.initials: T
authors.lastname: Gotohda
authors.forename: Y
authors.initials: Y
authors.lastname: Itoh
authors.forename: T
authors.initials: T
authors.lastname: Miyazaki
authors.forename: R A
authors.initials: RA
authors.lastname: Good
authors.forename: K
authors.initials: K
authors.lastname: Onoè
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000918:Antibody Specificity / Q000235:genetics / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D006389:Hemagglutinins, Viral / Q000737:chemistry / Q000276:immunology*; D066298:In Vitro Techniques; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology*; D007252:Influenza Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D008285:Major Histocompatibility Complex / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D006389:Hemagglutinins, Viral; D007252:Influenza Vaccines; D010446:Peptide Fragments; D014614:Vaccines, Synthetic