Terminal differentiation of mouse preadipocyte cells: adipogenic and antimitogenic role of triiodothyronine.
The role of triiodothyronine (T3) in the differentiation process of Ob1771 mouse preadipocyte cells has been studied under serum-free and hormone supplemented culture conditions which were previously shown to lead to terminal differentiation. In the absence of T3, a dramatic decrease in the adipogenic activity of the culture medium (EC50 = 0.1 nM) could be observed, as indicated 12 days after confluence by the low levels of late markers of differentiation such as adipsin, lipid-binding protein aP2 and glycerol-3-phosphate dehydrogenase as well as the sharp reduction of the number of triacyglycerol-containing cells. This decrease in adipogenic activity was accompanied by a parallel increase of the mitogenic potency of the culture medium. Therefore, T3 appears to be a hormone capable of modulating both proliferation and differentiation of preadipocytes. T3 ceased to be necessary provided the culture medium was supplemented with high concentrations of inducers of differentiation, such as 8-bromo-cAMP or carbaprostacyclin.
Darimont
C
C
Centre de Biochimie (UMR 134 CNRS), Université de Nice-Sophia Antipolis, France.
Gaillard
D
D
Ailhaud
G
G
Negrel
R
R
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002999:Clone Cells / Q000187:drug effects; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D011464:Epoprostenol / Q000031:analogs & derivatives / Q000494:pharmacology; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D015290:Second Messenger Systems; D014280:Triglycerides / Q000378:metabolism; D014284:Triiodothyronine / Q000494:pharmacology / Q000502:physiology*
D015415:Biomarkers; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D014280:Triglycerides; D014284:Triiodothyronine; D015124:8-Bromo Cyclic Adenosine Monophosphate; C026678:carboprostacyclin; D011464:Epoprostenol; D015056:1-Methyl-3-isobutylxanthine