Effect of dimethylsulfoxide and hydroxyethyl starch in the preservation of fractionated human marrow cells.
Fractionated human bone marrow cells, isolated by density centrifugation, could be well cryopreserved in both a DMSO/HES/albumin mixture and the conventional cryoprotectant (DMSO/serum) with either a programmed freezer or a -80 degrees C freezer. A lower initial temperature rise and a delayed plateau phase in the mixture in comparison with those in the DMSO/serum were observed using a programmed procedure without heat fusion compensation. Plateau phase in the mixture could also be shortened with increased liquid nitrogen to minimize the transition phase duration. However, the method of mononuclear cells immersed in a -80 degrees C freezer and stored in liquid nitrogen is simple and easily standardized. Additionally, widely used lactated Ringer's solution instead of Normosol-R can be employed to prepare the mixture for the preservation of fractionated cells.
Luo
K
K
Department of Medical Oncology, Cancer Hospital, Chinese Academy of Medical Sciences, Beijing.
Wu
G
G
Wang
Q
Q
Sun
Y
Y
Liu
H
H
D000418:Albumins; D001853:Bone Marrow; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D003114:Colony-Forming Units Assay; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D005069:Evaluation Studies as Topic; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives; D066298:In Vitro Techniques; D009369:Neoplasms / Q000628:therapy; D012996:Solutions; D014182:Transplantation, Autologous
D000418:Albumins; D003451:Cryoprotective Agents; D006895:Hydroxyethyl Starch Derivatives; D012996:Solutions; D004121:Dimethyl Sulfoxide