Pteridine biosynthesis and nitric oxide synthase in Physarum polycephalum.
Physarum polycephalum, an acellular slime mould, serves as a model system to study cell-cycle-dependent events since nuclear division is naturally synchronous. This organism was shown to release isoxanthopterin which is structurally related to tetrahydrobiopterin, a cofactor of aromatic amino acid hydroxylases and of nitric oxide synthases (NOSs) (EC 1.14.13.39). Here, we studied Physarum pteridine biosynthesis in more detail and found that high amounts of tetrahydrobiopterin are produced and NOS activity is expressed. Physarum pteridine biosynthesis is peculiar in as much as 7,8-dihydroneopterin aldolase (EC 4.1.2.25), an enzyme of folic acid biosynthesis usually not found in organisms producing tetrahydrobiopterin, is detected in parallel. NOS purified from Physarum depends on NADPH, tetrahydrobiopterin and flavins. Enzyme activity is independent of exogenous Ca2+ and is inhibited by arginine analogues. The purified enzyme (with a molecular mass of 130 kDa) contains tightly bound tetrahydrobiopterin and flavins. During the synchronous cell cycle of Physarum, pteridine biosynthesis increases during S-phase whereas NOS activity peaks during mitosis, drops at telophase and peaks again during early S-phase. Our results characterize Physarum pteridine biosynthesis and NOS and suggest a possible link between NOS activity and mitosis.
Werner-Felmayer
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Institute for Medical Chemistry and Biochemistry, University of Innsbruck, Austria.
Golderer
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Werner
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ER
Gröbner
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P
Wachter
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D000594:Amino Acid Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002453:Cell Cycle; D009249:NADP / Q000378:metabolism; D019001:Nitric Oxide Synthase; D016839:Physarum polycephalum / Q000378:metabolism*; D011621:Pteridines / Q000378:metabolism*
D011621:Pteridines; D009249:NADP; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases