title: Localization and inhibitory actions of galanin at the feline lower esophageal sphincter.
abstract: Intrinsic reflexes of the lower esophageal sphincter (LES) are mediated by specific arrangements of excitatory and inhibitory nerves. We have previously described an excitatory reflex at the feline LES mediated by a bombesin-like peptide (BN) which causes release of substance P (SP) to directly contract the LES. Galanin is a neurotransmitter in the enteric nervous system which colocalizes in neurons containing vasoactive intestinal peptide (VIP). The aims of this study were to determine: (1) the distribution of galanin at the feline LES; (2) the effect of galanin on basal LES tone; (3) the effect of galanin on agonist-induced LES contractions by BN, SP and bethanechol; and (4) the effect of galanin on LES relaxation induced by esophageal distension and exogenous VIP. Galanin-like immunoreactivity (galanin-LI) was localized in neurons that were widely distributed throughout the LES and adjacent organs. Galanin-LI was most abundant in the circular muscle, muscularis mucosa and myenteric plexus of the LES. In anesthetized cats, intra-arterial galanin had no effect on basal LES pressure in a dose range of 10(-11) to 10(-6) g/kg. Galanin (5.10(-7) g/kg) reduced the LES contractile response to SP by 65 +/- 8% (P = 0.0001). This galanin-mediated inhibition of SP was not blocked by tetrodotoxin. Galanin similarly decreased the LES contractile response to BN (63 +/- 7%, P = 0.005) and bethanechol (55 +/- 17%, P = 0.012). Galanin had no effect on the LES relaxation induced by esophageal distension or exogenous VIP. We conclude: (1) galanin-LI is present in neurons at the feline LES; (2) galanin has no effect on basal sphincter tone, but inhibits contractions of the LES by both direct and indirect agonists; and (3) galanin does not effect the LES relaxation induced by esophageal distension or VIP.
authors.affiliation: Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia.
authors.forename: G R
authors.initials: GR
authors.lastname: Lichtenstein
authors.forename: J C
authors.initials: JC
authors.lastname: Reynolds
authors.forename: C P
authors.initials: CP
authors.lastname: Ogorek
authors.forename: H P
authors.initials: HP
authors.lastname: Parkman
mesh_terms: D000818:Animals; D001839:Bombesin / Q000494:pharmacology; D002415:Cats; D004947:Esophagus / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D019004:Galanin; D005769:Gastrointestinal Motility / Q000187:drug effects / Q000502:physiology; D007150:Immunohistochemistry; D008297:Male; D008365:Manometry; D009130:Muscle, Smooth / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009479:Neuropeptides / Q000494:pharmacology* / Q000502:physiology; D010455:Peptides / Q000032:analysis / Q000494:pharmacology* / Q000502:physiology*; D013373:Substance P / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
chemical_list: D009479:Neuropeptides; D010455:Peptides; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D019004:Galanin; D001839:Bombesin