Interleukin-2 binding proteins in sera from normal subjects and multiple sclerosis patients.
We previously reported elevations of interleukin 2 (IL-2) in the serum of patients with chronic progressive MS. Using gel chromatography, protein A sepharose affinity chromatography, and ELISAs for IL-2 and the IL-2 soluble receptor, we now demonstrate that this cytokine is bound to serum proteins. These serum proteins include antibodies to IL-2, soluble IL-2 receptors, and high-molecular-weight proteins. Using a CTLL cell assay, a serum fraction corresponding to IgG antibodies to IL-2 inhibited the activity of this cytokine. Thus, we present evidence for potential immunomodulation of a pivotal cytokine in MS by serum proteins.
Trotter
J L
JL
Washington University School of Medicine, Department of Neurology and Neurosurgery, St. Louis, MO 63110, USA.
Damico
C A
CA
Trotter
A L
AL
Collins
K G
KG
Cross
A H
AH
D001798:Blood Proteins / Q000378:metabolism*; D002845:Chromatography / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism*; D009103:Multiple Sclerosis / Q000097:blood*
D001798:Blood Proteins; D007376:Interleukin-2