Developmental potential of rhesus monkey embryos produced by in vitro fertilization.
This study was an examination of the developmental potential of in vitro fertilization (IVF)-produced rhesus monkey embryos that were cultured in medium alone or cocultured with various cell types. End points were the quality and yield of embryos attaining the expanded or hatched blastocyst stage. A total of 96 IVF-produced embryos were cryopreserved and thawed, and 90 embryos were considered intact and suitable for culture. These embryos were placed into one of five treatment groups consisting of four different cell supports and medium alone. Two primary cultures (bovine oviductal cells [bOVID] and bovine cumulus cells [bCUM]) and two established cell lines (Vero cells and buffalo rat liver cells [BRL]) were utilized for coculture of embryos. Embryos were cultured for up to 14 days, and growth curves were established for all embryos that expanded and/or hatched. The developmental rate for embryos classified as viable varied substantially; in number of days to reach a given stage, early morulae ranged from Days 3 to 9 post-insemination, morulae from Days 4 to 9, blastocysts from Days 6 to 11, expanded blastocysts from Days 7 to 12, and hatched blastocysts from Days 9 to 15. On the basis of developmental curves, 30% of the embryos were arrested upon thawing or shortly after. Of the remaining embryos classified as viable, developmental efficiencies to the hatched blastocyst stage for the various treatments were 1) bOVID, 33%; 2) bCUM, 15%; 3) Vero cells, 9%; 4) BRL, 45%; and 5) medium alone, 8%.(ABSTRACT TRUNCATED AT 250 WORDS)
Zhang
L
L
Department of Animal Science, Louisiana State University, Baton Rouge 70803.
Weston
A M
AM
Denniston
R S
RS
Goodeaux
L L
LL
Godke
R A
RA
Wolf
D P
DP
D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D015925:Cryopreservation; D046508:Culture Techniques; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D008099:Liver; D008253:Macaca mulatta / Q000196:embryology*; D011247:Pregnancy; D013194:Staining and Labeling; D014709:Vero Cells