Beta 2-microglobulin-independent MHC class Ib molecule expressed by human intestinal epithelium.
A major histocompatibility complex class Ib protein, CD1d, is expressed by human intestinal epithelial cells (IECs) and is a ligand for CD8+ T cells. CD1d was found to be expressed on the surface of human IECs as a 37-kilodalton protein that was beta 2-microglobulin (beta 2M) independent with no N-linked carbohydrate. Transfection into a beta 2M- cell line confirmed that CD1d could be expressed at the cell surface in the absence of beta 2M. These data indicate that IECs use a specialized pathway for CD1d synthesis and that a beta 2M-independent class Ib protein may be the normal ligand for some intestinal T cells.
Balk
S P
SP
Hematology-Oncology Division, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215.
Burke
S
S
Polischuk
J E
JE
Frantz
M E
ME
Yang
L
L
Porcelli
S
S
Colgan
S P
SP
Blumberg
R S
RS
D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry; D018949:Antigens, CD1; D016827:CD8 Antigens; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D006031:Glycosylation; D006801:Humans; D015151:Immunoblotting; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology*; D008970:Molecular Weight; D011233:Precipitin Tests; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014162:Transfection; D001613:beta 2-Microglobulin / Q000502:physiology*
D015703:Antigens, CD; D018949:Antigens, CD1; D016827:CD8 Antigens; D001613:beta 2-Microglobulin