title: Pheromone response in yeast: association of Bem1p with proteins of the MAP kinase cascade and actin.
abstract: Haploid cells of the yeast Saccharomyces cerevisiae respond to mating pheromones with polarized growth toward the mating partner. This morphological response requires the function of the cell polarity establishment protein Bem1p. Immunochemical and two-hybrid protein interaction assays revealed that Bem1p interacts with two components of the pheromone-responsive mitogen-activated protein (MAP) kinase cascade, Ste20p and Ste5p, as well as with actin. Mutants of Bem1p that are associated with defective pheromone-induced polarized morphogenesis interacted with Ste5p and actin but not with Ste20p. Thus, the association of Bem1p with Ste20p and Ste5p may contribute to the conveyance of spatial information that regulates polarized rearrangement of the actin cytoskeleton during yeast mating.
authors.affiliation: Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec.
authors.forename: T
authors.initials: T
authors.lastname: Leeuw
authors.forename: A
authors.initials: A
authors.lastname: Fourest-Lieuvin
authors.forename: C
authors.initials: C
authors.lastname: Wu
authors.forename: J
authors.initials: J
authors.lastname: Chenevert
authors.forename: K
authors.initials: K
authors.lastname: Clark
authors.forename: M
authors.initials: M
authors.lastname: Whiteway
authors.forename: D Y
authors.initials: DY
authors.lastname: Thomas
authors.forename: E
authors.initials: E
authors.lastname: Leberer
mesh_terms: D000199:Actins / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D002352:Carrier Proteins; D016764:Cell Polarity; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D020930:MAP Kinase Kinase Kinases; D000072235:Mating Factor; D009024:Morphogenesis; D009154:Mutation; D010455:Peptides / Q000494:pharmacology; D010675:Pheromones / Q000494:pharmacology*; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D005656:Fungal Proteins; D047908:Intracellular Signaling Peptides and Proteins; D010455:Peptides; D010675:Pheromones; C080321:STE5 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C073129:BEM1 protein, S cerevisiae; D000072235:Mating Factor; D017346:Protein Serine-Threonine Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D020930:MAP Kinase Kinase Kinases; C078754:STE20 protein, S cerevisiae; D019204:GTP-Binding Proteins