Epitopes for human monoclonal antibodies and serotyping antisera against the O-specific polysaccharide of Pseudomonas aeruginosa O11.
Epitopes for Pseudomonas aeruginosa O11-specific human monoclonal antibodies (mAbs) and O11 serotyping antisera have been characterized. These mAbs recognized the O-polysaccharide portion of the lipopolysaccharide. The structure of the O-polysaccharide of O11 has been reported to be comprised of trisaccharide repeating-units as follows: -->3)-alpha-L-FucpNAc-(1-->1)-beta-D-FucpNAc-(1--> 2)-beta-D-Glcp-(1-->. (FucpNAc, 2-acetamido-2,6-dideoxygalactopyranoside.) Data from inhibition studies of binding in enzyme-linked immunosorbent assays and cell-agglutination assays, using monosaccharides and periodate-oxidized O-polysaccharide showed that the glucose residue, especially the C-3-C-6 segment and the beta-anomeric configuration, in the polysaccharide is essential for the epitopes of all anti-O11 mAbs; however, the detailed epitope specificities were different from one another. Furthermore, epitopes for serotyping antisera of O11 seemed to be similar to those for the human mAbs.
Yokota
S
S
Sumitomo Pharmaceuticals Research Center, Osaka, Japan.
Noguchi
H
H
D000371:Agglutination; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D007536:Isomerism; D008969:Molecular Sequence Data; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000276:immunology*; D012703:Serotyping; D014312:Trisaccharides / Q000737:chemistry* / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000939:Epitopes; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D014312:Trisaccharides