title: Recombinant vaccinia virus expressing PrM and E glycoproteins of louping ill virus: induction of partial homologous and heterologous protection in mice.
abstract: Recombinant vaccinia viruses expressing either the premembrane/truncated envelope (PrM/TrE) or truncated envelope (TrE) protein of louping ill virus were constructed. Both constructs expressed authentic E proteins as determined by their size and antigenic reactivity with a panel of monoclonal antibodies. The deletion of the C-terminal hydrophobic domain of the envelope glycoprotein resulted in the secretion of E protein into the supernatant culture medium. The immunisation of mice with these recombinant viruses showed that the recombinant expressing PrM/TrE proteins induced neutralising and protective antibodies against challenge with louping ill or tick-borne encephalitis virus, but that the recombinant expressing the E or the TrE protein alone failed to induce any detectable immune responses against homologous or heterologous virus challenge.
authors.affiliation: NERC Institute of Virology and Environmental Microbiology, Oxford.
authors.forename: K
authors.initials: K
authors.lastname: Venugopal
authors.forename: S Y
authors.initials: SY
authors.lastname: Shiu
authors.forename: E A
authors.initials: EA
authors.lastname: Gould
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000096:biosynthesis / Q000276:immunology*; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers; D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D007668:Kidney; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D011995:Recombination, Genetic; D017384:Sequence Deletion; D013552:Swine; D014162:Transfection; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D017931:DNA Primers; D000939:Epitopes; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins