Correlation between ultrastructural and histochemical parameters in lymphokine-activated killer (LAK) cells reacting with target cells in vitro.
Ultrastructural and fluorescence data allowed us to study the most important moments of the interaction between lymphokine-activated killer (LAK) cells against target cells (Chang) in vitro. The LAK cells, maintained at low doses of recombinant interleukin-2, were able to recognize, bind and destroy the tumoral cells. Before the attack, the LAK cells were characterized by a cytoplasm with a high ribosomes content; after the identification and the interaction cell-cell, a degeneration of the tumoral cell was observed. These observations allowed us to suppose that the interaction between the two types of cells may be mediated by a receptoral membrane system without the action of lytic enzymes.
Nano
R
R
Department of Animal Biology, University of Pavia, Italy.
Barni
S
S
Capelli
E
E
Cremaschi
P
P
Catanese
C
C
Tomaselli
S
S
Nascimbene
C
C
Prosperi
E
E
D001329:Autolysis; D002461:Cell Line, Transformed; D003602:Cytotoxicity, Immunologic / Q000502:physiology*; D004801:Eosine Yellowish-(YS); D006801:Humans; D007124:Immunoenzyme Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000201:enzymology* / Q000502:physiology / Q000648:ultrastructure*; D008751:Methylene Blue; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D007376:Interleukin-2; C043260:May-Grunwald Giemsa; D011994:Recombinant Proteins; D008751:Methylene Blue; D004801:Eosine Yellowish-(YS)