Insulin induces tyrosine phosphorylation of a 90-kDa protein during postischemic brain reperfusion.
Rat brain nuclear proteins were examined for tyrosine phosphorylation after resuscitation from a 10-min cardiac arrest. Insulin (1 unit/kg intravenously), given immediately after resuscitation, caused a marked increase in tyrosine phosphorylation of a 90-kDa brain protein. This effect occurred without hypoglycemia and was not observed after insulin administration in previously insulinopenic, diabetic, nonischemic animals. Insulin-responsive tyrosine phosphorylation of a specific 90-kDa protein during reperfusion may represent insulin stimulation of a neuroprotective brain response to an ischemic insult, consistent with recent observations that insulin administration during reperfusion protects selectively vulnerable neurons from postischemic death.
DeGracia
D J
DJ
Department of Emergency Medicine, Wayne State University School of Medicine, Detroit, Michigan 48201.
O'Neil
B J
BJ
White
B C
BC
Tiffany
B R
BR
Krause
G S
GS
Grossman
L I
LI
Grunberger
G
G
D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D006323:Heart Arrest / Q000378:metabolism* / Q000503:physiopathology; D007328:Insulin / Q000494:pharmacology*; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D015425:Myocardial Reperfusion; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D019000:Phosphotyrosine; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D012151:Resuscitation; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism
D007328:Insulin; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D019000:Phosphotyrosine; D014443:Tyrosine