title: Human peripheral blood granulocytes and myeloid leukemic cell lines express both transcripts encoding for stem cell factor.
abstract: Stem cell factor (SCF), the ligand for the c-kit proto-oncogene, has been shown to play a critical role in the migration of melanocytes and germ cells during embryogenesis as well as in the proliferative control of the hematopoietic compartment. In this study we investigated the expression of both the soluble and transmembrane SCF forms in purified peripheral blood populations and in several hematopoietic cell lines. Expression of both transcripts, though in different ratios, was identified in whole bone marrow, in bone marrow stromal cells and in human peripheral blood. In peripheral blood, SCF expression could be ascribable to polymorphonuclear leukocytes (PMN), whereas no SCF expression was detected in isolated lymphocytes, monocytes and in some T lymphoid cell lines. Conversely, some hematopoietic myeloid cell lines, such as HL-60, KG1 and K562, express SCF with similar patterns.
authors.affiliation: Istituto di Clinica Pediatrica Università di Torino, Italy.
authors.forename: U
authors.initials: U
authors.lastname: Ramenghi
authors.forename: L
authors.initials: L
authors.lastname: Ruggieri
authors.forename: I
authors.initials: I
authors.lastname: Dianzani
authors.forename: C
authors.initials: C
authors.lastname: Rosso
authors.forename: M F
authors.initials: MF
authors.lastname: Brizzi
authors.forename: C
authors.initials: C
authors.lastname: Camaschella
authors.forename: T
authors.initials: T
authors.lastname: Pietsch
authors.forename: G
authors.initials: G
authors.lastname: Saglio
mesh_terms: D001483:Base Sequence; D001773:Blood Cells / Q000378:metabolism; D001854:Bone Marrow Cells; D054278:Embryonal Carcinoma Stem Cells; D005091:Exons; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D015973:Gene Expression Regulation, Leukemic; D016298:Hematopoietic Cell Growth Factors / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D012995:Solubility; D019089:Stem Cell Factor; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D016298:Hematopoietic Cell Growth Factors; C000715470:MAS1 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D019089:Stem Cell Factor