[Modification of a panoptic method of staining isolated cells].
A simple and rapid method of isolated cell staining was achieved by modification of the Pappenheim's method. It is based on the following steps: (1) dilution of commonly used stock solutions May-Grünwald (1:1), Giemsa-Romanowsky (1.25% v/v), (2) reverse sequence of the used staining solutions in comparison with the original method, (3) shorter time of procedure (5 min/slide), (4) utilization of distilled water of pH 5.1-5.6 for dilution of solutions and rinsing of slides, (5) it is not necessary to add serum to cell suspensions. The presented histological method is suitable for cytomorphological examination of cells. It can be applied in routine hematological laboratories not equipped with cytospins. (Fig. 5, Ref. 6.).
Paulíková
E
E
Katedra fyziológie zivocichov a cloveka, PrF UPJS v Kosiciach.
Kociková
A
A
Sabol
M
M
D000818:Animals; D003584:Cytological Techniques; D051379:Mice; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*