title: Cloning and characterization of cDNAs encoding two normal isoforms of bovine stem cell factor.
abstract: The cDNA clones encoding two isoforms of bovine stem cell factor (bSCF) were obtained using reverse transcriptase-polymerase chain reaction, and their sequences were determined. The deduced amino acid sequences of the longer and shorter isoforms of bSCF consist, respectively, of 274 and 246 residues and show a high degree of identity to those of SCFs of different animal species. Northern blot analysis with the cDNA revealed the expression of a 5.8 kilobase bSCF RNA in fetal bovine tissues.
authors.affiliation: Institute of Society for Techno-innovation of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
authors.forename: J H
authors.initials: JH
authors.lastname: Zhou
authors.forename: H
authors.initials: H
authors.lastname: Hikono
authors.forename: M
authors.initials: M
authors.lastname: Ohtaki
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: M
authors.initials: M
authors.lastname: Sakurai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D016298:Hematopoietic Cell Growth Factors / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D019089:Stem Cell Factor
chemical_list: D018076:DNA, Complementary; D016298:Hematopoietic Cell Growth Factors; D019089:Stem Cell Factor