[Isolation and characteristics of an ordered library of transcribed sequences of human chromosome 19 from hybrid human-hamster cells].
A new technique based on Alu-PCR amplification of hn-RNA is described for the extraction of human-specific transcribed sequences from a hybrid cell line. Arrayed library of hn-cDNA was constructed and characterized by sequencing about 80 individual clones. A high enrichment by human-specific sequences (about 95%) was demonstrated.
Obradovich
D
D
Borodin
A M
AM
Kopantsev
E P
EP
Vagner
L L
LL
Volik
S V
SV
Ermolaeva
O D
OD
Lebedev
Iu B
IuB
Monastyrskaia
G S
GS
Sverdlov
E D
ED
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D006224:Cricetinae; D017931:DNA Primers; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D017931:DNA Primers; D012313:RNA