Tools for investigating functional interactions between ligands and G-protein-coupled receptors.
A general assay for evaluating functional interactions between ligands and G-protein-coupled receptors within minutes has been developed. The system uses the principles employed by animals such as reptiles, amphibians and fish to control their colors. In nature, activation of G-protein-coupled receptors expressed by skin cells called chromatophores effects pigment redistribution within the cells to change an animal's coloration. The in vitro 'chameleon in a dish' equivalent can use essentially any cloned G-protein-coupled receptor.
Lerner
M R
MR
Dept of Internal Medicine, Yale University School of Medicine, New Haven, CT 06536-0812.
D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D000242:Cyclic AMP / Q000502:physiology; D019204:GTP-Binding Proteins / Q000502:physiology*; D008024:Ligands; D008547:Melanophores / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D016297:Mutagenesis, Site-Directed; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology; D011898:Ranidae; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000235:genetics; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D012880:Skin Pigmentation / Q000502:physiology*
D008024:Ligands; D010455:Peptides; D011943:Receptors, Adrenergic, beta; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D000242:Cyclic AMP; D019204:GTP-Binding Proteins