Genotoxicity of kraft pulp spent liquors from different types of chlorination procedures.
The genotoxicity of spent liquors from kraft softwood and hardwood pulp bleaching processes was studied using the Ames Salmonella test and the SOS chromotest. The induction of micronuclei, in vivo, was assayed in bone marrow erythrocytes of B6 mice treated with softwood first chlorination stage spent liquor. The softwood bleaching process used a combination of Cl2 and ClO2 at the first chlorination stage. During the study the amount of free chlorine at the first chlorination stage in the softwood bleachery was gradually decreased, although the amount of active chlorine remained the same. Enzymatic bleaching was also used in a softwood process together with chlorine (Cl2 + ClO2). The hardwood bleaching plant used only ClO2 at the first chlorination stage. A decrease in genotoxicity, corresponding to the decrease in Cl2, was observed in the Ames Salmonella assays of the softwood bleaching plant effluents. A similar decrease was observed in the SOS chromotest. The highest decrease in mutagenic activity was observed when enzymatic bleaching was used together with chlorine.
Nylund
L
L
Institute of Occupational Health, Helsinki, Finland.
Rosenberg
C
C
Jäppinen
P
P
Vainio
H
H
D000818:Animals; D001711:Biotransformation; D001853:Bone Marrow / Q000187:drug effects / Q000648:ultrastructure; D002713:Chlorine; D007220:Industrial Waste; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens / Q000493:pharmacokinetics / Q000633:toxicity*; D010209:Paper; D013014:SOS Response, Genetics; D012486:Salmonella typhimurium / Q000235:genetics; D014934:Wood
D007220:Industrial Waste; D009153:Mutagens; D002713:Chlorine