Neither cyclosporin A nor FK506 affects adenylate cyclase responses of fetal rat keratinizing epidermal cells (FRSK cells) at concentrations that inhibit thymidine incorporation.
It has been reported that various anti-psoriatic agents augment the beta-adrenergic adenylate cyclase response that is defective in the psoriatic hyperproliferative epidermis. Recent evidence indicates that cyclosporin A (CsA) and FK506 show beneficial effects on psoriasis. Using fetal rat keratinizing epidermal cells (FRSK cells) we investigated the effects of these compounds on the adenylate cyclase system. These immunosuppressants had no effect on the adenylate cyclase responses of FRSK cells up to 1-10 microM concentration, although they significantly inhibited thymidine incorporation at concentrations more than 0.1 microM. There was no significant difference in the inhibitory effect on thymidine incorporation between CsA and FK506, despite that FK506 is a much more potent immunosuppressant than CsA.
Takahashi
H
H
Department of Dermatology, Asahikawa Medical College, Japan.
Iizuka
H
H
D000262:Adenylyl Cyclases / Q000032:analysis / Q000378:metabolism* / Q000502:physiology*; D000818:Animals; D002460:Cell Line; D016572:Cyclosporine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D000078404:Epidermal Cells; D004817:Epidermis / Q000196:embryology / Q000201:enzymology*; D005333:Fetus / Q000166:cytology; D051381:Rats; D016559:Tacrolimus / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism*
D016572:Cyclosporine; D000262:Adenylyl Cyclases; D013936:Thymidine; D016559:Tacrolimus