title: Distinguishing between duplex and hairpin forms of RNA by 15N-1H heteronuclear NMR.
abstract: A general method is described for distinguishing RNA hairpins from RNA duplexes by application of two-dimensional filtered nuclear Overhauser enhancement spectra on a 1:1 mixture of unlabeled and 99% 15N-labeled molecules. The method is applied to the RNA dodecamer rGGCGCUUGCGUC which can form an intramolecular hairpin under low salt conditions and a duplex in high salt. This procedure allows unambiguous identification of RNA hairpins or duplexes under the same conditions that are used in the NMR solution structure determination.
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215.
authors.forename: F
authors.initials: F
authors.lastname: Aboul-ela
authors.forename: E P
authors.initials: EP
authors.lastname: Nikonowicz
authors.forename: A
authors.initials: A
authors.lastname: Pardi
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*
chemical_list: D012313:RNA