Combination of cobalt labelling with immunocytochemical reactions for electron microscopic investigations on frog spinal cord.
Cobalt staining of primary afferents in frog spinal cord was combined with peroxidase-antiperoxidase pre-embedding or immunogold post-embedding immunocytochemical labelling. Our results have shown that cobalt labelling can easily be distinguished from both of the immunoreaction end products. The protocol of cobalt labelling did not affect the immunoreactivity of structures. The morphology and synaptology of cobalt labelled and immunostained profiles in our sections were very similar to those reported in previous studies using different double labelling techniques. These results indicate that this new combined method could be used as an alternative double labelling technique in electron microscopic studies on nervous tissues.
Nagy
I
I
Department of Anatomy, Histology and Embryology, University Medical School of Debrecen, Hungary.
Sik
A
A
Polgár
E
E
Petkó
M
M
Antal
M
M
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D003035:Cobalt; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry / Q000379:methods*; D016253:Microscopy, Immunoelectron; D009474:Neurons / Q000737:chemistry / Q000648:ultrastructure*; D011893:Rana esculenta; D013116:Spinal Cord / Q000737:chemistry / Q000648:ultrastructure*; D013194:Staining and Labeling; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D003035:Cobalt; D005680:gamma-Aminobutyric Acid; D015740:Calcitonin Gene-Related Peptide