A rapid immunostaining method for frozen sections.
A simple and rapid one-step method for demonstrating immunohistochemical markers (leukocyte common antigen, cytokeratin, etc.) is described, which can help define the nature of poorly differentiated neoplasms for diagnosis using frozen section. Microwave irradiation was used to speed immunohistochemical analysis using "Enhanced Polymer One-step Staining" (EPOS) reagents on cryostat sections from a variety of pathologic samples. Reproducible results were obtained using EPOS reagents for leukocyte common antigen and cytokeratin. The overall procedure takes less than 10 min and can be completed during surgery.
Chilosi
M
M
Department of Pathology, University of Verona, Italy.
Lestani
M
M
Pedron
S
S
Montagna
L
L
Benedetti
A
A
Pizzolo
G
G
Menestrina
F
F
D005629:Frozen Sections / Q000379:methods*; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis*; D017493:Leukocyte Common Antigens / Q000032:analysis*; D008198:Lymph Nodes / Q000737:chemistry; D008872:Microwaves; D009369:Neoplasms / Q000737:chemistry; D013950:Thymus Gland / Q000737:chemistry
D007633:Keratins; D017493:Leukocyte Common Antigens