title: In vivo binding of human inter-alpha-trypsin inhibitor free heavy chains to hyaluronic acid.
abstract: In vivo binding of human inter-alpha-trypsin inhibitor to hyaluronate was investigated by immunoelectrophoretic techniques. Pathological synovial fluids and follicular fluids both contain high concentrations of soluble hyaluronate. Heavy chain epitopes of inter-alpha-trypsin inhibitor were firmly associated with the hyaluronate in synovial fluid and follicular fluid. The hyaluronate-bound inter-alpha-trypsin inhibitor epitopes did not cross-react immunologically with bikunin. Several hyaluronate-bound inter-alpha-trypsin inhibitor fragments with molecular masses in the range 120,000-30,000 Da were demonstrated by immunoblotting. Heavy chain 1 of inter-alpha-trypsin inhibitor was shown to associate with hyaluronate by amino acid sequence analysis of isolated hyaluronate-bound proteins. These data indicate that in vivo metabolism of inter-alpha-trypsin inhibitor takes place in pathological synovial fluid and in ovarian follicular fluid shortly before ovulation.
authors.affiliation: Department of Clinical Chemistry, Holbaek Central Hospital, Denmark.
authors.forename: T E
authors.initials: TE
authors.lastname: Jessen
authors.forename: L
authors.initials: L
authors.lastname: Odum
authors.forename: A H
authors.initials: AH
authors.lastname: Johnsen
mesh_terms: D000510:Alpha-Globulins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000737:chemistry / Q000378:metabolism; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D008970:Molecular Weight; D006080:Ovarian Follicle / Q000378:metabolism; D010060:Ovulation; D011480:Protease Inhibitors / Q000378:metabolism; D012756:Sheep; D013582:Synovial Fluid / Q000378:metabolism; D014360:Trypsin Inhibitor, Kunitz Soybean; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
chemical_list: D000510:Alpha-Globulins; D000939:Epitopes; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D011480:Protease Inhibitors; C109148:SPINT2 protein, human; D014361:Trypsin Inhibitors; C011877:inter-alpha-inhibitor; D006820:Hyaluronic Acid; D014360:Trypsin Inhibitor, Kunitz Soybean; D002918:Chymotrypsin