Angiotensin II mediates intracellular signalling in vascular smooth muscle cells by activation of tyrosine-specific protein kinases and c-raf-1.
Angiotensin II induces protein tyrosine kinase activation and apparent decreased electrophoretic mobility of the c-raf-1 serine/threonine protein kinase in cultured rat vascular smooth muscle cells. Tyrosine phosphorylation of at least 9 cellular proteins with molecular weights of 151, 131, 116, 110, 90, 65, 62, 60, 52 kd was induced in a time- and concentration-dependent manner, and included a serine/threonine protein kinase. The phosphotyrosine containing proteins differed from those induced by PDGF BB or AB. Angiotensin II by itself was shown not to act as a mitogen in cultured smooth muscle cells.
Butcher
R D
RD
Institute of Molecular Cell Biology, University of Freiburg, FRG.
Schöllmann
C
C
Marmé
D
D
D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D007700:Kinetics; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism*; D019000:Phosphotyrosine; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019908:Proto-Oncogene Proteins c-raf; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D013997:Time Factors; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis
D010750:Phosphoproteins; D010982:Platelet-Derived Growth Factor; D011518:Proto-Oncogene Proteins; D000804:Angiotensin II; D019000:Phosphotyrosine; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D019908:Proto-Oncogene Proteins c-raf