Interleukin-1 beta induces differential adhesiveness on human endothelial cell surfaces.
The adhesive strength between HL-60 and endothelial cells activated with IL-1 beta was investigated according to the region (area) on the endothelial cell surface where the HL-60 cell was attached (nuclear, junctional, or cytoplasmic). Using a micropipette single-cell manipulation system, we demonstrated that the increase of adhesive force was due to the activation of the endothelial cell with IL-1 beta and a function of the region of the endothelial cell. The increase in adhesion strength due to the increased expression of E-Selectin on the endothelial cell was studied by the addition of monoclonal antibodies against E-Selectin, ICAM-1, VCAM, and PECAM-1. Our findings suggest that the greatest adhesive strength was seen in the junctional region where the antibodies could not block the adhesion. However, the contribution of E-Selectin was significant in the nuclear region.
Sung
K L
KL
Department of Orthopaedics, School of Medicine, University of California, San Diego, La Jolla 92093-0412.
Saldivar
E
E
Phillips
L
L
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology / Q000502:physiology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000502:physiology; D002478:Cells, Cultured; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007375:Interleukin-1 / Q000494:pharmacology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins; D019010:Vascular Cell Adhesion Molecule-1
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D007375:Interleukin-1; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1