Cloning and sequencing of an ovine granulocyte colony-stimulating factor cDNA.
A cDNA encoding an ovine (ov) granulocyte colony-stimulating factor (G-CSF) was cloned by polymerase chain reaction, using primers based on the 5' and 3' nucleotide (nt) sequence of the bovine (bov) G-CSF cDNA. RNA was isolated from IFN-gamma and LPS-stimulated alveolar macrophages. The isolated ovG-CSF cDNA is 522 bp in length and does not include coding sequence for a secretory signal peptide. The nt sequence is 95% identical to the bovG-CSF nt sequence, and includes conservation of 5 cysteine residues. The deduced amino acid (aa) sequence of the mature ovG-CSF protein shares 96%, 82% and 68% homology with the bov, human and murine mature G-CSF proteins, respectively. The estimated molecular weight of the 174 aa G-CSF protein is 18.8 kilodaltons.
O'Brien
P M
PM
CSIRO Division of Animal Health, Animal Health Research Laboratory, Parkville, Victoria, Australia.
Seow
H F
HF
Rothel
J S
JS
Wood
P R
PR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012756:Sheep
D018076:DNA, Complementary; D016179:Granulocyte Colony-Stimulating Factor