The effect of deoxynivalenol on serotoninergic neurotransmitter levels in pig blood.
Deoxynivalenol (DON) produces two characteristic toxicological effects, decreased feed consumption (anorexia) and emesis. Both effects have been linked to increased central (CNS) serotoninergic activity. Although there has also been some indication of a peripheral involvement, the role of blood pools of serotonin and related compounds in mediating DON toxicity is not well defined. In this study, the effect of DON on plasma concentrations of serotonin (5-hydroxytryptamine, 5HT), 5HIAA (5-hydroxyindoleacetic acid) and tryptophan (TRP), as a reflection of an induced peripheral serotoninergic system, was investigated in swine. Typical values for the plasma concentrations of 5HT, 5HIAA, and TRP were established in pigs. Following administration of DON, either intragastrically or intravenously, concentration changes in these substances were measured over an eight hour period. The effect of low and high toxin doses were also compared. Analyses showed no effect on plasma levels of the compounds of interest, even at sufficient toxin doses to invoke emesis in the test animals. Any variation over the course of the study remained within acceptable control limits. These results indicated no peripheral effect by DON which could account for the increased serotoninergic activity associated with altered feeding behaviour or emesis.
Prelusky
D B
DB
Centre for Food & Animal Research Agriculture and Agri-Food Canada, Ottawa, Ontario.
D000818:Animals; D006897:Hydroxyindoleacetic Acid / Q000097:blood*; D007275:Injections, Intravenous; D008297:Male; D012701:Serotonin / Q000097:blood*; D013270:Stomach; D013552:Swine / Q000097:blood*; D014255:Trichothecenes / Q000008:administration & dosage / Q000494:pharmacology*; D014364:Tryptophan / Q000097:blood*
D014255:Trichothecenes; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D014364:Tryptophan; C007262:deoxynivalenol