title: Modulation of immune response to Lol p I by pretreatment with anti-idiotypic antibody is not restricted to the idiotypic expression.
abstract: To study the role of anti-idiotypic antibodies in the regulation of the immune response to Lol p I (the major allergenic component of rye grass pollen), we have recently generated a panel of three MoAbs directed against distinct epitopes of Lolp I and an anti-idiotypic MoAb directed against the idiotype borne by one of the anti-Lol p I MoAbs (290A-167). The effects of pretreatment with this anti-idiotypic MoAb in BALB/c mice before immunization with the antigen have been examined. The anti-idiotypic MoAb or unrelated MoAb were given weekly for 8 weeks intraperitoneally. Mice then received the antigen (2 micrograms) adsorbed with alum (2 mg) at weeks 9, 11 and 13. Serum anti-Lol p I antibodies (IgG or IgE) and specific idiotypic responses were measured. Anti-Lol p I IgG antibodies could be detected before immunization with Lol p I only in mice pretreated with anti-idiotypic MoAb. Immunization with Lol p I induced an anti-Lol p I IgG response in both groups, but this response was higher in mice that received anti-idiotypic MoAb. Similar profiles were seen for specific IgE antibodies and idiotypic responses. Surprisingly, idiotypes borne by other anti-Lol p I MoAbs (539A-6 and 348A-6) had also been enhanced after pretreatment with the anti-290A-167 MoAb. These observations suggested that the pretreatment with this anti-idiotypic MoAb modulates not only the expression of the respective idiotype, but also affects other idiotype responses.
authors.affiliation: Centre de Recherche en Inflammation et Immunologie-Rhumatologie, Le Centre de Recherche du CHUL, Sainte-Foy, Québec, Canada.
authors.forename: Y
authors.initials: Y
authors.lastname: Boutin
authors.forename: J
authors.initials: J
authors.lastname: Hébert
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology*; D000917:Antibody Formation / Q000187:drug effects; D052179:Antigens, Plant; D000939:Epitopes / Q000276:immunology; D006967:Hypersensitivity / Q000517:prevention & control; D007114:Immunization; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010940:Plant Proteins / Q000276:immunology*; D011058:Pollen / Q000276:immunology*
chemical_list: D000485:Allergens; D000888:Antibodies, Anti-Idiotypic; D052179:Antigens, Plant; D000939:Epitopes; D007074:Immunoglobulin G; C042814:Lol p I protein, Lolium perenne; D010940:Plant Proteins; D007073:Immunoglobulin E