title: Enhanced secretion of endothelin by endothelial cells in response to hemoglobin.
abstract: Confluent cultures of bovine aortic endothelium in serum-free medium were exposed to increasing concentrations (10(-6)-10(-4) M) of freshly prepared erythrocyte lysates (primarily hemoglobin). Hemoglobin increased endothelin-1 secretion into the medium in a dose-dependent manner after 24 hours. The enhanced secretion of endothelin-1 in response to hemoglobin was sustained for 72 hours, suggesting active production and secretion of endothelin-1 rather than release from intracellular pools. Secreted endothelin-1 in the medium was characterized by high-performance liquid chromatography coupled with radioimmunoassay. Endothelin-1, a potent and long-lasting vasoconstrictor, may be one of the causative factors of cerebral vasospasm after subarachnoid hemorrhage. Oxyhemoglobin, derived from periarterial clot, may play an important role in the secretion of endothelin-1 in cerebral vasospasm.
authors.affiliation: Department of Neurosurgery, University of Washington, Seattle.
authors.forename: I
authors.initials: I
authors.lastname: Fuwa
authors.forename: M
authors.initials: M
authors.lastname: Mayberg
authors.forename: C
authors.initials: C
authors.lastname: Gadjusek
authors.forename: T
authors.initials: T
authors.lastname: Harada
authors.forename: Z
authors.initials: Z
authors.lastname: Luo
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D016232:Endothelins / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D006454:Hemoglobins / Q000502:physiology*; D006801:Humans; D010108:Oxyhemoglobins / Q000502:physiology
chemical_list: D016232:Endothelins; D006454:Hemoglobins; D010108:Oxyhemoglobins