The characterization of the O-antigen of Escherichia coli 064:K99 lipopolysaccharide.
The structure of the O-polysaccharide of the smooth lipopolysaccharide (LPS) produced by Escherichia coli O64:K99 was investigated by SDS-PAGE, composition, periodate oxidation, methylation, partial hydrolysis, and 1D and 2D nuclear magnetic resonance analyses, made on the native o-chain and its reduction and periodate degradation products. The E. coli O64 antigenic O-chain was found to be a high molecular weight glycan composed of D-galactose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-mannose (2:1:1:1) and was a polymer of branched pentasaccharide repeating units having the structure: [formula: see text]
Perry
M B
MB
Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
MacLean
L L
LL
Brisson
J R
JR
D000107:Acetylation; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000737:chemistry* / Q000254:growth & development / Q000276:immunology; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial