title: The genus-specific lipopolysaccharide epitope of Chlamydia is assembled in C. psittaci and C. trachomatis by glycosyltransferases of low homology.
abstract: Chlamydiae possess a genus-specific epitope that is located on the lipopolysaccharide (LPS) and is composed of a 3-deoxy-D-manno-octulosonic acid (Kdo) trisaccharide of the sequence alpha Kdo-(2-->8)--alpha Kdo-(2-->4)-alpha Kdo. In Chlamydia trachomatis, this trisaccharide is biosynthetically generated through the action of a multi-functional Kdo-transferase encoded by the gene gseA. gseA of Chlamydia psittaci 6BC was cloned and expressed in a rough mutant (Re chemotype) of Escherichia coli (strain F515) that contains an LPS with only two alpha 2-->4-linked Kdo residues. Recombinant strains were able to add the immunodominant Kdo residue in alpha 2-->8-linkage to the parental LPS, as determined by SDS-PAGE and Western blot analysis using a monoclonal antibody against the genus-specific epitope. The DNA sequence of gseA was determined and aligned to that published recently for C. trachomatis serovar L2. Most surprisingly, the two deduced amino acid sequences shared only an overall homology of 67%. Thus, gseA exhibits species specificity at the DNA level, whereas its gene product results in the synthesis of a carbohydrate antigen with genus specificity.
authors.affiliation: Institut für Experimentelle Biologie und Medizin, Forschungsinstitut Borstel, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Mamat
authors.forename: M
authors.initials: M
authors.lastname: Baumann
authors.forename: G
authors.initials: G
authors.lastname: Schmidt
authors.forename: H
authors.initials: H
authors.lastname: Brade
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002240:Carbohydrate Sequence; D002692:Chlamydia trachomatis / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002691:Chlamydophila psittaci / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D000939:Epitopes / Q000096:biosynthesis / Q000235:genetics; D005798:Genes, Bacterial; D008070:Lipopolysaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014166:Transferases / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D014166:Transferases; C019917:3-deoxy-D-manno-octulosonate transferase