[Effect of antibodies specific to sarcolemma of cardiomyocytes in activity of 5-nucleotidase in rats].
Rabbit antibodies specific to sarcolemma of cardiomyocytes have been studied for their effect on activity of 5'-nucleotidase ecto-enzyme of the Wistar rat cardiomyocytes. It is shown that incubation of isolated plasma membranes of cardiomyocytes of rats (100 micrograms of protein) with the gamma-globulin fraction of antisarcolemmal cardial serum (gamma-ASCS, 20 micrograms protein) and with the gamma-globulin fraction of normal rabbit serum (gamma-NRS, 0.20 micrograms protein) promotes a decrease of the enzyme activity. An inhibiting effect was greatly pronounced in incubation with gamma-NRS and did not depend on the dose of antibodies and duration of their action. An assumption is made that inhibiting action of antibodies on activity of ecto-5'-nucleotidase may change adenosine concentration and may be one of elements in the mechanism promoting an increase of the intracellular calcium concentration.
Aleksiuk
L I
LI
Ianchiĭ
R I
RI
Pidzilia
Iu P
IuP
Sukhina
V S
VS
D015720:5'-Nucleotidase / Q000378:metabolism*; D000818:Animals; D000906:Antibodies; D001798:Blood Proteins / Q000378:metabolism; D002478:Cells, Cultured; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012508:Sarcolemma / Q000201:enzymology*; D005719:gamma-Globulins / Q000378:metabolism
D000906:Antibodies; D001798:Blood Proteins; D005719:gamma-Globulins; D015720:5'-Nucleotidase