title: Molecular cloning of the canine CD44 antigen cDNA.
abstract: Molecular cloning of the dog homologue of the human CD44 was achieved using RT/PCR. A 1055 bp cDNA has a deduced amino acid sequence of 351 residues, 338 of them correspond to the mature protein. Nine conserved cysteine residues were found. The extracellular region contains a single link superfamily domain on the N-terminal part and potential post-translational modification sites as: N- and O-linked glycosylation sites and chondroitin sulfate attachment sites. Three mRNAs of 2.2, 3.8 and 4.4 kb were identified on Northern blot analysis and Western blot hybridization revealed a 85-90 kDa protein expressed in lymph node tissue.
authors.affiliation: Diabetes Research Institute, University of Miami School of Medicine, FL 33101.
authors.forename: K F
authors.initials: KF
authors.lastname: Milde
authors.forename: R
authors.initials: R
authors.lastname: Alejandro
authors.forename: D H
authors.initials: DH
authors.lastname: Mintz
authors.forename: R L
authors.initials: RL
authors.lastname: Pastori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000378:metabolism; D003001:Cloning, Molecular; D004285:Dogs; D006031:Glycosylation; D006801:Humans; D018960:Hyaluronan Receptors; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing / Q000235:genetics* / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D002809:Chondroitin Sulfates