title: Involvement of the galactosyl-1-phosphate transferase encoded by the Salmonella enterica rfbP gene in O-antigen subunit processing.
abstract: rfbT of Salmonella enterica LT2 was previously thought, together with rfaL, to be involved in the ligation of polymerized O antigen to core-lipid A, and three mutants were known. We report the mapping of the mutations to rfbP, the galactosyl-1-phosphate transferase gene, which is now shown to encode a bifunctional protein. The mutations which have the former rfbT phenotype are referred to as rfbP(T). We also show that rfbP(T) mutants are not blocked in the ligation step as previously believed but in an earlier step, possibly in flipping the O-antigen subunit on undecaprenyl pyrophosphate from the cytoplasmic to periplasmic face of the cytoplasmic membrane.
authors.affiliation: Department of Microbiology, University of Sydney, New South Wales, Australia.
authors.forename: L
authors.initials: L
authors.lastname: Wang
authors.forename: P R
authors.initials: PR
authors.lastname: Reeves
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D002240:Carbohydrate Sequence; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D019081:O Antigens; D011106:Polyisoprenyl Phosphates / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000378:metabolism*; D012475:Salmonella / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D019081:O Antigens; D011106:Polyisoprenyl Phosphates; D011135:Polysaccharides, Bacterial; C082559:RfbT protein, Vibrio cholerae; C088139:undecaprenyl pyrophosphate; D017855:Transferases (Other Substituted Phosphate Groups); C088138:undecaprenyl-phosphate galactosephosphotransferase