Utility of fragmented human fetal tissue as a potential dopaminergic brain graft in Parkinson's disease.
There is increasing interest in the use of human fetal dopaminergic tissue as a source of striatal transplant in parkinsonian patients. This tissue is acquired by elective abortions. The possibilities of the use of this tissue were studied by macroscopical examination, cell-culturing followed by immunohistochemical staining and by high performance liquid chromatography. It turned out that 50% of the curettages obtained by suction abortion were too fragmented to reliably recognize the dopamine-containing area (ventral mesencephalon). Furthermore, dissection of the brainstem immediately after the abortion procedure seemed to be of utmost importance.
HogenEsch
R I
RI
Department of Neurology, University Hospital of Groningen, The Netherlands.
Staal
M J
MJ
Kema
I P
IP
Buys
C H
CH
Go
K G
KG
D016380:Brain Tissue Transplantation; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000637:transplantation; D004298:Dopamine / Q000032:analysis / Q000502:physiology*; D016332:Fetal Tissue Transplantation; D006801:Humans; D007150:Immunohistochemistry; D010300:Parkinson Disease / Q000601:surgery*; D013194:Staining and Labeling; D014019:Tissue Donors
D004298:Dopamine