Antigen-specific proliferative response of peritoneal exudate lymphocytes primed with antigen and bacterial lipopolysaccharide: the roles of Ia+ accessory cells and IL-2.
In vitro antigen-specific proliferation was investigated in a lymphocyte population that had been taken from the peritoneal exudate cells (PEC) of C3H/HeN mice (Iak) primed in vivo with both bacterial lipopolysaccharide (LPS) and horse red blood cells (HRBC) and had been purified by passage through a nylon fiber column (Nfc). The proliferative response of the Nfc-passed lymphocytes primed with HRBC and LPS [T(HRBC+LPS) cells] depended on the dose of antigen in the cultures, and the response was higher than that of cells prepared from mice primed with HRBC alone [T(HRBC) cells]. No response was seen in the cells prepared from the LPS-primed mice [T(LPS) cells] or normal mice [T(N) cells]. The response of the T(HRBC) cells was abolished by previous treatment of the cells with anti-Iak antibody and complement (C), whereas the response of the T(HRBC+LPS) cells was retained after the same treatment, indicating that the Ia- T(HRBC+LPS) cells can proliferate in response to antigen in spite of Ia+ accessory cell-depletion. Supernatants from the cultures of Ia- T(HRBC+LPS) cells in the presence of HRBC showed abundant IL-2 activity, while those of Ia- T(HRBC) cells did not. The IL-2 should be produced by the L3T4 cell population in T(HRBC+LPS) cells in response to antigen, since the previous treatment of the cells with anti-L3T4 antibody and C abrogated the production.(ABSTRACT TRUNCATED AT 250 WORDS)
Nitta
T
T
Department of Bacteriology, Ohu University School of Dentistry, Koriyama, Japan.
Imai
H
H
Ogasawara
Y
Y
Hirayama
N
N
Nakano
M
M
D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology*; D000939:Epitopes / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000032:analysis* / Q000502:physiology; D006736:Horses / Q000276:immunology; D007376:Interleukin-2 / Q000502:physiology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010529:Peritoneal Cavity / Q000166:cytology; D012756:Sheep / Q000276:immunology
D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D008070:Lipopolysaccharides