title: Prevalence of HPV in premalignant and malignant cervical lesions in Greenland and Denmark: PCR and in situ hybridization analysis on archival material.
abstract: Archival formalin-fixed paraffin-embedded cervical specimens from 125 women in Greenland and 139 women in Denmark who had CIN I-III or cervical cancer diagnosed between 1983 and 1987 were analysed for human papillomavirus type 16 (HPV-16) by in situ hybridization and for HPV-16, 18, 31, 33, 35 and 45 by PCR. In situ hybridization analysis showed an HPV-16 prevalence of 17% (16/95) and 23% (24/105) in the premalignant lesions from Greenland and Denmark, respectively. The HPV-16 prevalence rate in the cancer specimens was 10% (3/30) in the samples from Greenland and 29% (10/34) in the Danish specimens. A total of 82 Greenlandic and 107 Danish specimens were beta-globin-positive by PCR reaction. HPV-16-specific PCR on these samples showed 63% (34/54) of the Greenlandic and 68% (50/74) of the Danish preinvasive lesions to be positive. The corresponding HPV-16-positive rates for the invasive cancers were 82% (23/28) for Greenland and 70% (23/33) for Denmark. This study of patient samples supports our previous population-based studies in which similar HPV-detection rates were found among random samples of women from Greenland and Denmark, although Greenland has a 4-5-fold higher cervical cancer incidence.
authors.affiliation: DNA Tumor Virus Laboratory, Institute of Medical Microbiology, University of Copenhagen.
authors.forename: A M
authors.initials: AM
authors.lastname: Sebbelov
authors.forename: C
authors.initials: C
authors.lastname: Svendsen
authors.forename: H
authors.initials: H
authors.lastname: Jensen
authors.forename: S K
authors.initials: SK
authors.lastname: Kjaer
authors.forename: B
authors.initials: B
authors.lastname: Norrild
mesh_terms: D000328:Adult; D001483:Base Sequence; D001609:Beta-Globulins / Q000235:genetics; D004279:DNA, Viral / Q000032:analysis; D003718:Denmark / Q000453:epidemiology; D005260:Female; D006116:Greenland / Q000453:epidemiology; D006801:Humans; D017403:In Situ Hybridization; D008875:Middle Aged; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000150:complications / Q000453:epidemiology / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D014412:Tumor Virus Infections / Q000150:complications / Q000453:epidemiology / Q000382:microbiology*; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000382:microbiology*; D002578:Uterine Cervical Dysplasia / Q000150:complications / Q000382:microbiology*
chemical_list: D001609:Beta-Globulins; D004279:DNA, Viral