Anti-HIV activity of dideoxynucleosides, foscarnet and fusidic acid is potentiated by human leukocyte interferon in blood-derived macrophages.
Blood-derived macrophages were acutely infected with HIV and treated with a combination of leucocyte interferon (IFN) and five anti-HIV drugs. HIV growth was assayed by quantitation of p24 antigen in the supernatant and in some experiments by determination of reverse transcriptase activity. Both IFN and all drugs, with the exception of fusidic acid, inhibited HIV growth in a dose-dependent manner. IFN in combination with zidovudine, dideoxycytidine or fusidic acid exerted a synergistic effect on HIV titers, while IFN combined with dideoxyinosine or foscarnet had an additive effect.
Degré
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M
Institute of Bacteriology, Rikshospitalet, University of Oslo, Norway.
Beck
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S
D002478:Cells, Cultured; D016049:Didanosine / Q000494:pharmacology; D015224:Dideoxynucleosides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D017245:Foscarnet / Q000494:pharmacology*; D005672:Fusidic Acid / Q000494:pharmacology*; D006678:HIV / Q000187:drug effects* / Q000254:growth & development; D016655:HIV Core Protein p24 / Q000032:analysis; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology*; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D014779:Virus Replication; D016047:Zalcitabine / Q000494:pharmacology; D015215:Zidovudine / Q000494:pharmacology
D015224:Dideoxynucleosides; D016655:HIV Core Protein p24; D016898:Interferon-alpha; D017245:Foscarnet; D015215:Zidovudine; D005672:Fusidic Acid; D016047:Zalcitabine; D012194:RNA-Directed DNA Polymerase; D016049:Didanosine