Defective CD3 mediated proliferation and LPS responsiveness in multiple sclerosis.
Multiple sclerosis [MS] is a chronic inflammatory disease of the central nervous system which has been postulated to be a T cell mediated disease. We examined proliferation of mononuclear cells to OKT3 mAb, Con A, ionomycin plus PMA and human myelin basic protein in subjects with relapsing-remitting and chronic progressive multiple sclerosis. Age and sex matched controls demonstrated a good proliferation to anti-CD3 mAb whereas subjects with relapsing-remitting multiple sclerosis showed a significantly decreased anti-CD3 mAb response. There was no difference in mitogen, ionomycin plus PMA or human MBP proliferation between controls and MS subjects. There was also a trend for decreasing anti-CD3 mAb proliferation in patients with chronic progressive multiple sclerosis compared to controls. LPS significantly decreased anti-CD3 mAb proliferation in controls but not in the MS subjects. An abnormality of signal transduction via the CD3 T-cell receptor complex in T cells and responsiveness to the immunomodulatory effect of IFN inducers may exist in multiple sclerosis.
Brod
S A
SA
Department of Neurology, University of Texas Southwestern Medical Center at Dallas 75235.
Scott
M
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D017252:CD3 Complex / Q000276:immunology*; D005260:Female; D006801:Humans; D016898:Interferon-alpha / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D009103:Multiple Sclerosis / Q000145:classification / Q000276:immunology*; D004676:Myelin Basic Protein / Q000276:immunology / Q000302:isolation & purification; D011070:Poly I-C / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D017252:CD3 Complex; D016898:Interferon-alpha; D008070:Lipopolysaccharides; D004676:Myelin Basic Protein; D011070:Poly I-C