Induction of nitric oxide synthase in human mammary arteries in vitro.
This study investigated whether human mammary arteries express an inducible nitric oxide (NO) synthase and, if so, what its effects are on vascular tone. In human mammary artery pre-contracted with phenylephrine there was a gradual time-dependent loss of tone over an 8 h period. L-Arginine and lipopolysaccharide enhanced the rate but not the magnitude of this loss in tone, whereas NG-nitro-L-arginine, NG-monomethyl-L-arginine, dexamethasone, and polymyxin B inhibited these effects. These findings indicate that incubation of human mammary artery with lipopolysaccharide resulted in the expression of an inducible NO synthase. The induction of this enzyme in human vessels may be important in the pathogenesis of septic shock.
Berrazueta
J R
JR
Department of Internal Medicine, University Hospital M. Valdecilla, Cantabria University, Santander, Spain.
Salas
E
E
Amado
J A
JA
Sánchez de Vega
M J
MJ
Poveda
J J
JJ
D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis*; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology; D004790:Enzyme Induction / Q000187:drug effects; D005938:Glucocorticoids / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D008323:Mammary Arteries / Q000187:drug effects / Q000201:enzymology*; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D019001:Nitric Oxide Synthase; D019335:Nitroarginine; D010656:Phenylephrine / Q000494:pharmacology; D019323:omega-N-Methylarginine
D005938:Glucocorticoids; D010656:Phenylephrine; D019335:Nitroarginine; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D019331:NG-Nitroarginine Methyl Ester