Co-existence of PACAP and nitric oxide synthase in the rat hypothalamus.
The possible co-existence of pituitary adenylate cyclase-activating polypeptide (PACAP)-38 and nitric oxide synthase (NOS) in the rat hypothalamus was examined by a combination of PACAP-immunocytochemistry and NADPH-diaphorase histochemistry. Virtually all PACAP-38-immunoreactive neurones in the paraventricular and supraoptic nuclei exhibited NADPH-diaphorase activity. Since NADPH-diaphorase activity was identical to NOS-immunoreactivity in the magnocellular neurosecretory neurones, this finding indicates that the PACAP neurones synthesize NO.
Okamura
H
H
Department of Anatomy, Kyoto Prefectural University of Medicine, Japan.
Miyagawa
A
A
Takagi
H
H
Esumi
H
H
Yanaihara
N
N
Ibata
Y
Y
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001479:Basal Ganglia / Q000201:enzymology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007031:Hypothalamus / Q000201:enzymology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009479:Neuropeptides / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D019001:Nitric Oxide Synthase; D010286:Paraventricular Hypothalamic Nucleus / Q000201:enzymology / Q000378:metabolism; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051381:Rats; D017208:Rats, Wistar; D013495:Supraoptic Nucleus / Q000201:enzymology / Q000378:metabolism
C495403:Adcyap1 protein, rat; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases