VCAM-1 is a receptor for encephalomyocarditis virus on murine vascular endothelial cells.
Murine VCAM-1 has been identified as a receptor for the D variant of encephalomyocarditis (EMC-D) virus on vascular endothelial cells from the heart. Monoclonal antibodies to VCAM-1 inhibited infection and lysis of endothelial cells with EMC-D virus. CHO cells transfected with the VCAM-1 gene were susceptible to EMC-D virus lysis, while control CHO cells transfected with the ELAM-1 gene were resistant. Similarly, 35S-labeled EMC-D virus bound to CHO-VCAM cells, and binding was inhibited with anti-VCAM-1 antibodies. Little or no radiolabeled virus bound to CHO-ELAM-1 cells.
Huber
S A
SA
Department of Pathology, University of Vermont, Burlington 05405.
D000818:Animals; D000911:Antibodies, Monoclonal; D016466:CHO Cells; D018188:Cardiovirus Infections / Q000209:etiology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006224:Cricetinae; D004686:Encephalomyocarditis virus / Q000472:pathogenicity / Q000502:physiology*; D004730:Endothelium, Vascular / Q000382:microbiology* / Q000502:physiology; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D011991:Receptors, Virus / Q000037:antagonists & inhibitors / Q000276:immunology / Q000502:physiology*; D014162:Transfection; D019010:Vascular Cell Adhesion Molecule-1
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D011991:Receptors, Virus; D019010:Vascular Cell Adhesion Molecule-1