Determination of streptomycin and dihydrostreptomycin in animal tissue by on-line sample enrichment liquid chromatography.
A method for the determination of streptomycin and dihydrostreptomycin in pork and bovine muscle and kidney was developed. Dilute perchloric acid solution is used to precipitate proteins and extract the analytes from the tissue. The extract is loaded onto a cation-exchange, solid-phase extraction column, and the drugs are eluted with pH 8 phosphate buffer. The eluant is chromatographed by using an on-line column enrichment liquid chromatographic system with postcolumn derivatization using 1,2-naphthoquinone-4-sulfonic acid and detection by fluorescence. The recoveries were 61.1% (coefficient of variation [CV], 7.3%) for streptomycin and 55.3% (CV, 8.2%) for dihydrostreptomycin. The detection limits were 10 ppb for streptomycin and 20 ppb for dihydrostreptomycin.
Gerhardt
G C
GC
Agriculture Canada, Health of Animals Laboratory, Saskatoon, SK.
Salisbury
C D
CD
MacNeil
J D
JD
D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods; D004096:Dihydrostreptomycin Sulfate / Q000032:analysis*; D007668:Kidney / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*; D013307:Streptomycin / Q000032:analysis*; D013552:Swine
D004096:Dihydrostreptomycin Sulfate; D013307:Streptomycin