title: Conserved initiator proteins in eukaryotes.
abstract: The origin recognition complex (ORC), a multisubunit protein identified in Saccharomyces cerevisiae, binds to chromosomal replicators and is required for the initiation of cellular DNA replication. Complementary DNAs (cDNAs) encoding proteins related to the two largest subunits of ORC were cloned from various eukaryotes. The cDNAs encoding proteins related to S. cerevisiae Orc1p were cloned from the budding yeast Kluyveromyces lactis, the fission yeast Schizosaccharomyces pombe, and human cells. These proteins show similarity to regulators of the S and M phases of the cell cycle. Genetic analysis of orc1+ from S. pombe reveals that it is essential for cell viability. The cDNAs encoding proteins related to S. cerevisiae Orc2p were cloned from Arabidopsis thaliana, Caenorhabditis elegans, and human cells. The human ORC-related proteins interact in vivo to form a complex. These studies studies suggest that ORC subunits are conserved and that the role of ORC is a general feature of eukaryotic DNA replication.
authors.affiliation: Cold Spring Harbor Laboratory, NY 11724, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Gavin
authors.forename: M
authors.initials: M
authors.lastname: Hidaka
authors.forename: B
authors.initials: B
authors.lastname: Stillman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D017173:Caenorhabditis elegans / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004261:DNA Replication; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007716:Kluyveromyces / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D051738:Origin Recognition Complex; D016133:Polymerase Chain Reaction; D018741:Replication Origin; D012097:Repressor Proteins / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000737:chemistry / Q000235:genetics / Q000254:growth & development
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C497214:ORC1 protein, S cerevisiae; C497223:ORC1 protein, human; D051738:Origin Recognition Complex; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins