title: Identification of rare and novel mutations in the CFTR genes of CF patients in southern England.
abstract: Cystic fibrosis patients referred to two genetics centres in southern England and not found to carry common CF-associated mutations in one or both of their CFTR genes have been subjected to an extensive mutation search. The whole of the coding region of the CFTR gene, all intron-exon boundaries and 5' and 3' untranslated regions have been examined by a combination of single stranded conformational polymorphism analysis and chemical mismatch detection; 48 chromosomes with rare mutations have been identified, including 7 novel mutations, 182delT in exon 1, G27X in exon 2, Q151X in exon 4, Q220X in exon 6a, Q525X in exon 10, 3041delG in exon 16, and 4271delC in exon 23.
authors.affiliation: Institute of Molecular Medicine, Oxford University, John Radcliffe Hospital.
authors.forename: S
authors.initials: S
authors.lastname: Shackleton
authors.forename: J
authors.initials: J
authors.lastname: Hull
authors.forename: S
authors.initials: S
authors.lastname: Dear
authors.forename: A
authors.initials: A
authors.lastname: Seller
authors.forename: A
authors.initials: A
authors.lastname: Thomson
authors.forename: A
authors.initials: A
authors.lastname: Harris
mesh_terms: D001483:Base Sequence; D003550:Cystic Fibrosis / Q000453:epidemiology / Q000235:genetics*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D004252:DNA Mutational Analysis; D004739:England / Q000453:epidemiology; D005787:Gene Frequency; D005796:Genes; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: C505032:CFTR protein, human; D008565:Membrane Proteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator