title: [Recent developments in the diagnosis of parapoxviruses].
abstract: Neutralizing and non-neutralizing monoclonal antibodies against parapoxviruses (PPV) were generated by immunizing BALB/c-mice with gradient-purified PPV Orf D-1701 or purified envelopes. Epitope specificity studies identified three distinct epitopes localized in the virus envelope. These antigenic sites allowed a differentiation between orf and stomatitis papulosa viruses. For a rapid diagnosis of parapoxviruses transmission-electron microscopy, immunofluorescence- or immunoperoxidase-staining, antigen capture ELISA, and polymerase-chain-reaction were used.
authors.affiliation: Institut für Medizinische Mikrobiologie, Infektions- und Seuchenmedizin, Tierärztliche Fakultät der Ludwig-Maximilians-Universität München.
authors.forename: C P
authors.initials: CP
authors.lastname: Czerny
authors.forename: R
authors.initials: R
authors.lastname: Waldmann
authors.forename: M
authors.initials: M
authors.lastname: Büttner
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology; D002417:Cattle; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008168:Lung / Q000166:cytology / Q000196:embryology; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron; D009923:Orf virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D018157:Parapoxvirus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D016133:Polymerase Chain Reaction; D011213:Poxviridae Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D011817:Rabbits; D012756:Sheep
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D004279:DNA, Viral; D000939:Epitopes