Assessment of myointimal cellular kinetics in a model of angioplasty by means of proliferating cell nuclear antigen expression.
A detailed temporal assessment of cellular proliferation was carried out by means of immunostaining for proliferating cell nuclear antigen in a normolipemic rabbit model of balloon angioplasty to the iliac arteries. Assessment was made at 30 minutes, 2 hours, 1 day, 3 days, 7 days, 14 days, 1 month and 3 months after the procedure. Intimal hyperplasia was first noted at day 3; a prominent layer was formed by day 14. Cellular proliferation in the vessel media was observed as early as day 1 (percentage of positive-staining cells 0.5% +/- 0.2%), reaching a maximum by day 7 (16.9% +/- 5.1%) before returning to baseline levels by 1 month (0.2% +/- 0.02%); in the intima, cellular proliferation was first noted at day 7 (0.7% +/- 0.3%) and reached a maximum at day 14 (4.1% +/- 0.4%) before returning to baseline levels at 1 month (0.3% +/- 0.1%). Use of proliferating cell nuclear antigen expression in this model of angioplasty provided a simple and reproducible method of assessing cellular proliferation after vascular injury and may prove useful for monitoring the effects, in experimental models, of agents for reducing myointimal hyperplasia.
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R S
RS
Academic Department of Cardiology, Glenfield General Hospital, Leicester, United Kingdom.
Rutty
G
G
Underwood
M J
MJ
Brack
M J
MJ
Gershlick
A H
AH
D000800:Angioplasty, Balloon / Q000009:adverse effects; D000818:Animals; D001324:Autoantigens / Q000032:analysis*; D002452:Cell Count; D002453:Cell Cycle; D002455:Cell Division; D006965:Hyperplasia; D007150:Immunohistochemistry; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D011817:Rabbits; D013194:Staining and Labeling; D017539:Tunica Intima / Q000166:cytology* / Q000473:pathology; D017540:Tunica Media / Q000276:immunology / Q000473:pathology*
D001324:Autoantigens; D018809:Proliferating Cell Nuclear Antigen