Studies on lipid oxidation in fish phospholipid liposomes.
Fish phospholipid liposomes were prepared and used as an artificial membrane system to study factors influencing lipid oxidation. The extent of lipid oxidation was indexed by measuring the amount of thiobarbituric acid reactive substances (TBARS) produced. Fe2+, Fe3+, and Cu2+ were potent prooxidants in catalysing lipid oxidation. These metal ions induced lipid oxidation in a dose dependent manner. However, Zn2+, Ni2+, and Mn2+ did not significantly (p > 0.05) affect lipid oxidation at all the concentrations (1, 10, or 100 microM) studied. Morin, luteolin (flavonoids), butein (chalcone), tannic acid, ellagic acid (polyphenols), butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) (synthetic antioxidants) were potent antioxidants (producing < 50% TBARS compared to control) of Fe(2+)-catalyzed lipid oxidation. Morin, luteolin, and butein possess two hydroxyl substituents, a C4 ketone structure and a 2-3 double bond, all of which contributed to their antioxidative potential. Fe2+ caused some losses of polyunsaturated fatty acids (PUFA), whereas tannic acid protected the oxidation of several of the PUFA including C 16:1 (Palmitoleic acid), C 18:3 (Linolenic acid), C 20:4 (Arachidonic acid), C 20:5 (Eicosapentaenoic acid), and C 22:6 (Docosahexaenoic acid).
Ramanathan
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Department of Biochemistry, Faculty of Medicine, National University of Singapore.
Das
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D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology; D005227:Fatty Acids / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005290:Ferric Compounds / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology; D005399:Fishes / Q000378:metabolism*; D050356:Lipid Metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008081:Liposomes / Q000378:metabolism*; D010084:Oxidation-Reduction; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D005290:Ferric Compounds; D005296:Ferrous Compounds; D008081:Liposomes; D017392:Thiobarbituric Acid Reactive Substances; C020748:ferrous sulfate; D003300:Copper