Chondroitin sulphates and embryonic chick skin fibroblast proliferation.
We have investigated the action of sulphated glycosaminoglycans (GAGs) upon primary 11-day chick embryo skin fibroblasts cultured for various periods of time in the presence or absence of fetal calf serum (FCS). Chondroitin 4- and 6-sulphate (CS) added to serum supplemented medium provoked a decrease in DNA synthesis both in sparse and crowded cultures only at high concentrations (250 micrograms/ml). Synthesis of endocellular and secreted proteins was not affected. In contrast, CS administered to fibroblasts for 24 h in serum-free medium stimulated DNA synthesis. We postulate that the CS stimulating effect seen in serum-free cultures might be antagonized by peptide regulators of cell growth normally present in serum.
Bodo
M
M
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Università degli Studi di Perugia, Italy.
Pezzetti
F
F
Giammarioli
M
M
Bellucci
C
C
Baroni
T
T
Becchetti
E
E
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D014176:Protein Biosynthesis; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000196:embryology
D003470:Culture Media; D016895:Culture Media, Serum-Free; D002809:Chondroitin Sulfates; D004247:DNA