Destaining of nitrocellulose blots after staining with silver or colloidal gold.
A new method for destaining blots stained with colloidal gold is described. After destaining and restaining, artifacts and discolorations caused by the colloidal gold can often be removed. The silver produced by silver enhancement can also be selectively removed without affecting the gold stain.
Nelson
T J
TJ
National Institute of Health, NINDS, Neural Systems Section, Bethesda, Maryland 20892.
D000818:Animals; D016477:Artifacts; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003101:Collodion; D003102:Colloids; D049832:Decapodiformes; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006046:Gold; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000302:isolation & purification; D012834:Silver; D013194:Staining and Labeling
D003102:Colloids; D009419:Nerve Tissue Proteins; D012834:Silver; D006046:Gold; D003101:Collodion