Isolation of human CD34+ peripheral blood stem cells using an immunomagnetic positive selection system: prior monocyte depletion using a nylon-wool column.
An immunomagnetic separation system has been used to collect CD34+ cells in mobilized blood after treatment with a nylon-wool column. Cell purities were increased from 2.6% preseparation to 94.6% postseparation, with a mean yield 45.2% (n = 4). Forty percent of CD34+ cells separated by the immunomagnetic procedure formed colonies in the presence of hematopoietic growth factors in a limiting dilution assay. Eleven percent of these clones proliferated to over 10(5) cells and contained megakaryocytes.
Nakamura
Y
Y
Blood Transfusion Center, Tokai University Hospital, Kanagawa, Japan.
Noma
M
M
Kidokoro
M
M
Kobayashi
N
N
Takei
M
M
Kurashima
S
S
Mukaiyama
T
T
Kato
S
S
D015703:Antigens, CD / Q000097:blood*; D018952:Antigens, CD34; D002454:Cell Differentiation / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D002999:Clone Cells; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation*; D007201:Indicator Dilution Techniques; D007958:Leukocyte Count; D009000:Monocytes / Q000166:cytology; D009757:Nylons
D015703:Antigens, CD; D018952:Antigens, CD34; D009757:Nylons