Genetic and molecular analysis of the rpoD gene from Lactococcus lactis.
A gene of Lactococcus lactis ATCC19435, the product of which is homologous with the principal sigma factors of Escherichia coli and Bacillus subtilis, was cloned and sequenced. The deduced amino acid sequence of the 340-residue protein and the upstream open reading frame of the cloned gene showed a homology to B. subtilis sigma 43 factor (the rpoD product) and DNA primase (the dnaE product), respectively, suggesting that L. lactis also has the rpoD operon. Surprisingly, introduction of the cloned L. lactis rpoD gene into a rpoD temperature-sensitive mutant of E. coli caused partial complementation.
Araya
T
T
Nutritional Science Laboratory, Morinaga Milk Industry Co., Ltd., Kanagawa, Japan.
Ishibashi
N
N
Shimamura
S
S
Tanaka
K
K
Takahashi
H
H
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D019915:DNA Primase; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D013294:Lactococcus lactis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon; D012316:RNA Nucleotidyltransferases / Q000737:chemistry / Q000235:genetics; D015183:Restriction Mapping; D012808:Sigma Factor / Q000737:chemistry / Q000235:genetics*; D013696:Temperature
D001426:Bacterial Proteins; D012808:Sigma Factor; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases