A method for utilizing biocytin to study retinofugal pathways at the light and electron microscopic levels.
Numerous methods have been utilized in the past to study the retinofugal pathway at both the light and electron microscopic levels. However, many of these techniques have technical drawbacks that make them difficult to use in electron microscopic studies. We present herein a method for utilizing the anterograde tracer biocytin to study the retinal pathways at both the light and electron microscopic levels. Biocytin is an especially useful tracer since it clearly labels very small axons and boutons in addition to the larger fibers. In addition, the synaptic ultrastructure is left intact and the technique can be utilized in numerous double-labeling neuroanatomical studies.
Helm
G A
GA
Department of Neurological Surgery, University of Virginia Health Sciences Center, Charlottesville 22908.
Palmer
P E
PE
Simmons
N E
NE
diPierro
C G
CG
Ebbesson
S O
SO
D000818:Animals; D001370:Axonal Transport; D008239:Lysine / Q000031:analogs & derivatives*; D008853:Microscopy; D008854:Microscopy, Electron; D009900:Optic Nerve / Q000648:ultrastructure*; D011894:Rana pipiens; D013194:Staining and Labeling; D014795:Visual Pathways / Q000648:ultrastructure*
C013411:biocytin; D008239:Lysine