title: E-selectin supports neutrophil rolling in vitro under conditions of flow.
abstract: E-selectin was evaluated for its ability to support neutrophil adhesion under conditions of flow. At a wall shear stress of 1.85 dyn/cm2, neutrophils were found to attach to E-selectin expressed on the apical surface of L cell monolayers. The initial intercellular contact was most often evidenced by neutrophils rolling on the monolayer at a mean rate of congruent to 10 microns/s. Anti-E-selectin monoclonal antibody, CL2/6, inhibited this interaction by > 90%. Rolling neutrophils often transiently stopped, but in contrast to the behavior on stimulated endothelial cells, they remained spherical in shape and did not migrate on or beneath the monolayer. A possible contribution of neutrophil L-selectin to this interaction was indicated by the findings that anti-L-selectin monoclonal antibody, DREG-56, inhibited E-selectin-dependent adhesion under flow by > 65%, and there was a highly significant correlation between surface levels of L-selectin and E-selectin-dependent adhesion under flow. E-selectin also appeared to support neutrophil adhesion to IL-1 beta-stimulated endothelial cells under conditions of flow, but it accounted for only congruent to 30% of the level of adherence, in contrast to L-selectin which accounted for > 65%. Thus, both L-selectin and E-selectin can support neutrophil adhesion at wall shear stresses that preclude intercellular adhesion molecule-1-dependent adhesion, and they participate in neutrophil adherence to stimulated endothelial cells under conditions of flow.
authors.affiliation: Biomedical Engineering Laboratory, Rice University, Houston, Texas 77251.
authors.forename: O
authors.initials: O
authors.lastname: Abbassi
authors.forename: T K
authors.initials: TK
authors.lastname: Kishimoto
authors.forename: L V
authors.initials: LV
authors.lastname: McIntire
authors.forename: D C
authors.initials: DC
authors.lastname: Anderson
authors.forename: C W
authors.initials: CW
authors.lastname: Smith
mesh_terms: D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000097:blood; D018821:CD18 Antigens; D002448:Cell Adhesion / Q000187:drug effects*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000502:physiology*; D006801:Humans; D007739:L Cells; D051379:Mice; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D014162:Transfection; D014471:Umbilical Veins
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D019040:E-Selectin