A modified hepatitis B virus surface antigen with the receptor-binding site for hepatocytes at its C terminus: expression, antigenicity and immunogenicity.
A modified hepatitis B virus (HBV) surface antigen, the SA-28 protein, was constructed and expressed by recombinant vaccinia virus in mammalian cells. This protein was composed of a PreS1 region-derived peptide (amino acids 21 to 47) that contained the hepatocyte receptor-binding site, joined to the C terminus of the major S protein at amino acid position 223. This modified surface antigen could be efficiently assembled into particles with a density of 1.23 g/ml and could be secreted from several mammalian cell lines. The results of immunoprecipitation revealed that the SA-28 protein was recognized by both the anti-S protein antibody and the anti-PreS1 antibody. A strong antibody response, against both the S protein and PreS1 epitopes, was induced in BALB/c mice immunized by the SA-28 particles indicating good immunogenicity. These results suggested that the HBV surface antigen consisting of the SA-28 protein could be a promising candidate as a new HBV vaccine with higher efficacy.
Xu
X
X
Shanghai Institute of Biochemistry, Academia Sinica, People's Republic of China.
Li
G D
GD
Kong
Y Y
YY
Yang
H L
HL
Zhang
Z
Z
Cao
H T
HT
Wang
Y
Y
D000818:Animals; D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D002460:Cell Line; D000939:Epitopes / Q000276:immunology; D015870:Gene Expression; D006508:Hepatitis Antibodies / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology; D008099:Liver / Q000166:cytology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D011498:Protein Precursors / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011946:Receptors, Antigen / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology*; D014611:Vaccination; D014616:Vaccinia virus / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines
D000939:Epitopes; D006508:Hepatitis Antibodies; D006514:Hepatitis B Surface Antigens; D011498:Protein Precursors; D011946:Receptors, Antigen; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C030500:hepatitis B surface antigen receptor; C050806:presurface protein 1, hepatitis B surface antigen