Reduction of tumor necrosis factor production by splenocytes from v-Ha-ras oncogene-bearing mice.
Mice containing the activated v-Ha-ras oncogene driven by the mouse mammary tumor virus promoter/enhancer produced less tumor necrosis factor (TNF) than genetically identical animals without it. Inbred Oncomice containing the v-Ha-ras oncogene and inbred FVB mice without it were grown for 6 months. Splenocytes were isolated and stimulated in vitro to produce tumor necrosis factor (TNF) and gamma-interferon (IFN). TNF production by cells from Oncomice was significantly decreased compared to cells from FBV mice. There was a tendency for decrease, but no significant difference was seen on IFN release. These observations suggest that the oncogene may play a role in the immune system.
Chen
G J
GJ
Department of Family and Community Medicine, University of Arizona, Tucson 85624.
Colombo
L L
LL
Lopez
M C
MC
Watson
R R
RR
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D011905:Genes, ras; D007372:Interferons / Q000096:biosynthesis; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D014409:Tumor Necrosis Factor-alpha; D007372:Interferons