Expression of interleukin-11 and its encoding mRNA by glioblastoma cells.
Interleukin-11 (IL-11) is a pleiotropic cytokine with important effects on hematopoietic and other cells. IL-11 was originally described as a product of stromal cell lines and fibroblasts. Using RT-PCR, Northern blotting, and ELISA we demonstrated that the human U373 and U87 glioblastoma cell lines expressed IL-11 and its encoding mRNA when stimulated with IL-1 beta, phorbol ester, and calcium ionophore. The neuroblastoma cell line SH-SY5Y did not express IL-11 mRNA in response to these agents. Cerebral expression of IL-11 by glial cells is important because IL-11 has been shown to have effects on neuronal electrophysiology, has overlapping functions with the neuroactive cytokine interleukin-6, and is part of the gp130-associated neuropoietic family of cytokines.
Murphy
G M
GM
Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, CA 94305-5485, USA.
Bitting
L
L
Majewska
A
A
Schmidt
K
K
Song
Y
Y
Wood
C R
CR
D001253:Astrocytes / Q000378:metabolism*; D015152:Blotting, Northern; D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D004357:Drug Synergism; D004797:Enzyme-Linked Immunosorbent Assay; D005815:Genetic Code; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D017370:Interleukin-11 / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D007375:Interleukin-1; D017370:Interleukin-11; D012333:RNA, Messenger; D000001:Calcimycin; D013755:Tetradecanoylphorbol Acetate