title: Design of degenerate oligonucleotide primers for cloning of G-protein alpha subunits.
authors.affiliation: Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.
authors.forename: T M
authors.initials: TM
authors.lastname: Wilkie
authors.forename: A M
authors.initials: AM
authors.lastname: Aragay
authors.forename: A J
authors.initials: AJ
authors.lastname: Watson
authors.forename: M I
authors.initials: MI
authors.lastname: Simon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000138:chemical synthesis*; D018076:DNA, Complementary / Q000096:biosynthesis; D019204:GTP-Binding Proteins / Q000096:biosynthesis*; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis; D016133:Polymerase Chain Reaction / Q000379:methods*; D012194:RNA-Directed DNA Polymerase; D011994:Recombinant Proteins / Q000096:biosynthesis*; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D016376:Oligonucleotides, Antisense; D011994:Recombinant Proteins; D012194:RNA-Directed DNA Polymerase; D019204:GTP-Binding Proteins