title: Correlation between ultrastructural and histochemical parameters in lymphokine-activated killer (LAK) cells reacting with target cells in vitro.
abstract: Ultrastructural and fluorescence data allowed us to study the most important moments of the interaction between lymphokine-activated killer (LAK) cells against target cells (Chang) in vitro. The LAK cells, maintained at low doses of recombinant interleukin-2, were able to recognize, bind and destroy the tumoral cells. Before the attack, the LAK cells were characterized by a cytoplasm with a high ribosomes content; after the identification and the interaction cell-cell, a degeneration of the tumoral cell was observed. These observations allowed us to suppose that the interaction between the two types of cells may be mediated by a receptoral membrane system without the action of lytic enzymes.
authors.affiliation: Department of Animal Biology, University of Pavia, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Nano
authors.forename: S
authors.initials: S
authors.lastname: Barni
authors.forename: E
authors.initials: E
authors.lastname: Capelli
authors.forename: P
authors.initials: P
authors.lastname: Cremaschi
authors.forename: C
authors.initials: C
authors.lastname: Catanese
authors.forename: S
authors.initials: S
authors.lastname: Tomaselli
authors.forename: C
authors.initials: C
authors.lastname: Nascimbene
authors.forename: E
authors.initials: E
authors.lastname: Prosperi
mesh_terms: D001329:Autolysis; D002461:Cell Line, Transformed; D003602:Cytotoxicity, Immunologic / Q000502:physiology*; D004801:Eosine Yellowish-(YS); D006801:Humans; D007124:Immunoenzyme Techniques; D007376:Interleukin-2 / Q000494:pharmacology; D015979:Killer Cells, Lymphokine-Activated / Q000201:enzymology* / Q000502:physiology / Q000648:ultrastructure*; D008751:Methylene Blue; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D007376:Interleukin-2; C043260:May-Grunwald Giemsa; D011994:Recombinant Proteins; D008751:Methylene Blue; D004801:Eosine Yellowish-(YS)