Inhibition of platelet spreading from plasma onto glass by an adsorbed layer of a novel fluorescent-labeled poly(ethylene oxide)/poly(butylene oxide) block copolymer: characteristics of the exclusion zone probed by means of polystyrene beads and macromolecules.
We have investigated the anti-adhesive properties of a newly synthesized fluorescent triblock copolymer containing poly(ethylene oxide). This adsorbs from aqueous solution onto glass that has been rendered hydrophobic. When the polymer-treated surface was exposed to human platelet-rich plasma (PRP) or whole blood at 37 degrees C, platelet adhesion and spreading were prevented. Avid adhesion and rapid platelet spreading occurred along tracks scraped in the adsorbed polymer coating, as seen by video-enhanced interference reflection microscopy. Leukocytes from whole blood are eventually able to adhere to the polymer-treated surface and were seen to remove labeled polymer from their vicinity and accumulate it at the cell body. Interferometry using polystyrene spheres showed that they do not adhere to polymer-coated glass and are unable to approach closer than 70-95 nm. On scraped tracks, beads make molecular contacts with the glass. Because the fully extended solvated (EO)400 arms may extend up to 100 nm from the glass, this suggests that the polymer forms a monolayer with the hydrophilic arms projecting into the water, whereas the hydrophobic (BO)55 segment binds the molecule to the hydrophobic surface. Another tri-bloc copolymer with shorter hydrophilic arms allows particles to approach more closely.
Gingell
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D
Department of Anatomy and Developmental Biology, University College London, United Kingdom.
Owens
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N
D000327:Adsorption; D001792:Blood Platelets / Q000187:drug effects* / Q000648:ultrastructure; D003911:Dextrans; D004852:Epoxy Compounds / Q000494:pharmacology*; D005452:Fluoresceins; D005453:Fluorescence; D005898:Glass; D006801:Humans; D066298:In Vitro Techniques; D008863:Microspheres; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011137:Polystyrenes
D003911:Dextrans; D004852:Epoxy Compounds; D005452:Fluoresceins; D011137:Polystyrenes; C087757:poly(ethylene oxide)-poly(butylene oxide) copolymer; D011092:Polyethylene Glycols