The LacZ transgene in MutaMouse maps to chromosome 3.
Transgenic mouse models are being used with increasing frequency for mutational and toxicological studies. One such system. MutaMouse, contains a stably integrated lambda-gt10LacZ shuttle vector in the mouse genome. We describe the use of dual color fluorescence in situ hybridization (FISH) with Mus musculus whole chromosome paints and lambda DNA to map the integration site of the lambda transgene to band C on mouse chromosome 3.
Swiger
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RR
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94551.
Myhr
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B
Tucker
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JD
D000818:Animals; D002874:Chromosome Mapping; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D007763:Lac Operon / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*