A novel factor, TA20, involved in neuronal differentiation: cDNA cloning and expression.
Combined treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA) and dibutyryl cyclic AMP (diBu-cAMP) induced significantly longer neurites than treatment with each alone in NG108-15 cells. We performed differential screening to identify genes expressed only by treatment with TPA plus diBu-cAMP but not by that with diBu-cAMP for 72 h alone in NG108-15 cells, and isolated a novel gene, TA20. Over-expression of the gene in NG108-15 and neuroblastoma N18TG-2 cells caused intense neurite elongation and suppressed cell growth. TA20 did not cause, however, any morphological changes in glioma C6Bu-1 cells. These results suggest that TA20 is a novel neuronal differentiation factor.
Tohda
C
C
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Nagai
S
S
Tohda
M
M
Nomura
Y
Y
D000818:Animals; D015152:Blotting, Northern; D002454:Cell Differentiation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D005796:Genes / Q000502:physiology*; D016501:Neurites / Q000502:physiology*; D012333:RNA, Messenger / Q000096:biosynthesis; D014162:Transfection
D018076:DNA, Complementary; D012333:RNA, Messenger; D000242:Cyclic AMP