Identification of new apolipoprotein B epitopes and haplotypes and their distribution in swine populations.
Results from comparative immunogenetic studies on inheritance and identification of four new apolipoprotein B (apoB) allotypes and three additional apoB haplotypes and their distribution in miniature and domestic swine are presented. Immunological surveys on the four new and 16 previously described Lpb allotypes and genetic analysis of their segregation in progenies, of miniature and domestic swine and their crosses, indicate that three new allotypes designated Lpb9, Lpb10 and Lpb101 are individual (mutant) apoB epitopes, each representing a discriminating marker for one of the new apoB haplotypes specified by three new apoB alleles designated Lpb9, Lpb10 and Lpb101. The fourth allotype, Lpb20, is one of the common epitopes forming the alternative epitope pair with Lpb10, and is a constituent of each of the eight previously described and two new apoB haplotypes. The new apoB alleles have so far been found only in miniature swine, with Lpb10 being the most frequent in the Göttingen, Vietnamese Pot-belly and Japanese Miniature, Lpb9 was detected only in Minnesota Miniature and Lpb101 only in Vietnamese Potbelly. The common allotype, Lpb20, shares immunological similarities with human apoB indicating its ancestral origin, whereas none of the alloreagents detecting the three individual apoB variants, Lpb9, Lpb10 or Lpb101, showed cross-reactivity with human apoB, suggesting their exclusive swine origin and evolvement during speciation through mutations.
Rapacz
J
J
Department of Genetics, University of Wisconsin, Madison 53706.
Hasler-Rapacz
J O
JO
Hu
Z L
ZL
Rapacz
J M
JM
Vögeli
P
P
Hojný
J
J
Janik
A
A
D000483:Alleles; D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics* / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D005828:Genetics, Population; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D009154:Mutation; D010641:Phenotype; D011110:Polymorphism, Genetic; D013552:Swine / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D013556:Swine, Miniature / Q000097:blood / Q000235:genetics / Q000276:immunology
D001055:Apolipoproteins B; D000939:Epitopes