Induction of phagocytic activity of M1 cells by an inhibitor of vacuolar H+-ATPase, bafilomycin A1.
Bafilomycin A1, a selective inhibitor of vacuolar H+-ATPase, time- and dose-dependently induced the differentiation of M1 cells, a murine myeloid leukemic cell line, into macrophage-like cells as revealed by the phagocytosis of polystyrene latex particles. This differentiation was inhibited not only by actinomycin D and cycloheximide but also by ST-638 (an inhibitor of tyrosine kinase). However, it was affected neither by K-252a (an inhibitor of C-kinase) nor by H-89 (an inhibitor of A-kinase), in contrast to the M1 cell differentiation induced by leukemia inhibitory factor (LIF). Okadaic acid inhibited both the bafilomycin A1-induced and LIF-induced differentiation of M1 cells.
Kinoshita
K
K
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kanazaw University, Ishikawa, Japan.
Hidaka
H
H
Ohkuma
S
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002227:Carbazoles / Q000494:pharmacology; D002934:Cinnamates / Q000494:pharmacology; D004988:Ethers, Cyclic / Q000494:pharmacology; D006131:Growth Inhibitors / Q000494:pharmacology; D026121:Indole Alkaloids; D015850:Interleukin-6; D007546:Isoquinolines / Q000494:pharmacology; D053605:Leukemia Inhibitory Factor; D007951:Leukemia, Myeloid; D008222:Lymphokines / Q000494:pharmacology; D018942:Macrolides; D008264:Macrophages / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D019319:Okadaic Acid; D010587:Phagocytosis / Q000187:drug effects*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D014176:Protein Biosynthesis; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D047428:Protein Kinase Inhibitors; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D012313:RNA / Q000096:biosynthesis; D013440:Sulfides / Q000494:pharmacology; D013449:Sulfonamides; D014407:Tumor Cells, Cultured; D014617:Vacuoles / Q000201:enzymology*
D000900:Anti-Bacterial Agents; D002227:Carbazoles; D002934:Cinnamates; D004988:Ethers, Cyclic; D006131:Growth Inhibitors; D026121:Indole Alkaloids; D015850:Interleukin-6; D007546:Isoquinolines; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D018942:Macrolides; D047428:Protein Kinase Inhibitors; D013440:Sulfides; D013449:Sulfonamides; C060747:ST 638; D019319:Okadaic Acid; D012313:RNA; C040929:bafilomycin A1; C049985:staurosporine aglycone; D011493:Protein Kinase C; D010749:Phosphoprotein Phosphatases; D006180:Proton-Translocating ATPases; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide