A spot test for protein detection and semiquantitative estimation in small samples.
Modern biological experiments, conducted on mini- and micro-scales require methods for protein detection in small samples. The method proposed here is based on visual inspection of protein dots immobilized on nitrocellulose filters and stained with the common protein dye amido black. It allows detection and semiquantitative determination of protein concentrations in a wide range.
Zlatanova
J
J
Department of Biochemistry and Biophysics, Oregon State University, Corvallis, 97331-7503.
Paneva
E
E
Yaneva
J
J
D000580:Amido Black; D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D003574:Cytochrome c Group / Q000032:analysis; D006657:Histones / Q000032:analysis; D008099:Liver / Q000737:chemistry; D051379:Mice; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013194:Staining and Labeling
D003574:Cytochrome c Group; D006657:Histones; D011506:Proteins; D012710:Serum Albumin, Bovine; D000580:Amido Black