Monoclonal antibodies raised against membrane glycoproteins from mouse brain recognize N-linked oligomannosidic glycans.
Monoclonal L3 and L4 antibodies have been shown to recognize carbohydrate epitopes on several neural cell adhesion molecules; these epitopes can be released by treatment with endoglycosidase H. In the present study, we have identified the oligosaccharides released by endoglycosidase H from the cell adhesion molecules AMOG and L1 by fast-atom bombardment mass spectrometry as being solely of the oligomannosidic type. Using neoglycolipids of oligomannosidic glycans, we also report that both antibodies show the highest reactivity with the alpha-manno-pentaose Man alpha 1-3-[Man alpha 1-6(Man alpha 1-3)Man alpha 1-6]-Man, but decreasing reactivity with the alpha-manno-hexaose, heptaose, octaose and nonaose glycans. Thus, to our knowledge, we describe here for the first time monoclonal antibodies recognizing N-glycosidically linked oligomannosidic glycans.
Schmitz
B
B
Department of Neurobiology, Swiss Federal Institute of Technology, Zürich.
Peter-Katalinic
J
J
Egge
H
H
Schachner
M
M
D000251:Adenosine Triphosphatases; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D001666:Binding Sites, Antibody; D002240:Carbohydrate Sequence; D027682:Cation Transport Proteins; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D016326:Extracellular Matrix Proteins / Q000737:chemistry; D005643:Fucose / Q000737:chemistry; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009844:Oligosaccharides / Q000276:immunology; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D017026:Swainsonine / Q000494:pharmacology
D000911:Antibodies, Monoclonal; C504191:Atp1b2 protein, mouse; D027682:Cation Transport Proteins; D015816:Cell Adhesion Molecules, Neuronal; D000939:Epitopes; D016326:Extracellular Matrix Proteins; D008562:Membrane Glycoproteins; D009844:Oligosaccharides; D011134:Polysaccharides; C028569:oligomannoside; D005643:Fucose; D000251:Adenosine Triphosphatases; D017026:Swainsonine