Modified RNA sequence pools for in vitro selection.
We report the use of modified RNA, in which the 2'-OH group of pyrimidines is replaced by a 2'-amino (2'-NH2) group to identify high affinity ligands specific for human neutrophil elastase (HNE) by in vitro selection. Compared to unmodified RNA the 2'-NH2-modified RNA ligands show enhanced stability in human serum and urine. Use of RNase T1 cleavage data in the presence of K+ and Li+ ions suggests that the modified RNA ligands selected for HNE form an intermolecular G-quartet structure.
Lin
Y
Y
NeXagen, Inc., Boulder, CO 80301.
Qiu
Q
Q
Gill
S C
SC
Jayasena
S D
SD
D000595:Amino Acid Sequence; D001483:Base Sequence; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019272:Leukocyte Elastase; D008024:Ligands; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012313:RNA / Q000097:blood / Q000737:chemistry* / Q000378:metabolism* / Q000652:urine; D011869:Radioligand Assay
D008024:Ligands; D012313:RNA; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase