title: New modified substrates for discriminating between human DNA polymerases alpha and epsilon.
abstract: Two 2'-deoxynucleoside 5'-alpha-methylenephosphonyl-beta, gamma-diphosphates were synthesized. They were incorporated into the DNA chain by DNA polymerase alpha from human placenta. Meanwhile, they were not recognized by DNA polymerase epsilon and beta of the same origin as well as by reverse transcriptases from human immunodeficiency virus and avian myeloblastosis virus.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
authors.forename: M V
authors.initials: MV
authors.lastname: Jasko
authors.forename: D G
authors.initials: DG
authors.lastname: Semizarov
authors.forename: L S
authors.initials: LS
authors.lastname: Victorova
authors.lastname: Mozzherin DJu
authors.forename: A A
authors.initials: AA
authors.lastname: Krayevsky
authors.forename: M K
authors.initials: MK
authors.lastname: Kukhanova
mesh_terms: D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006678:HIV / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D009711:Nucleotides; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase