title: Molecular cloning of the testicular follicle stimulating hormone receptor of the non human primate Macaca fascicularis and identification of multiple transcripts in the testis.
abstract: Reverse transcription PCR was used to amplify the complete open reading frame of the follicle-stimulating hormone receptor (FSHR) from testicular poly (A)+ RNA of the non-human primate Macaca fascicularis. Along with the structural motifs of a G-protein coupled receptor, sequence analysis reveals that the monkey FSHR is highly homologous to the human FSHR and has specific features such as N-linked glycosylation sites which are identical to the human FSHR but not present in the rat or ovine FSHR. Northern blot hybridization of testicular poly (A)+ RNA to a cRNA probe corresponding to the extracellular domain of the monkey FSHR resulted in the identification of several transcripts, indicating alternative splicing events of the primary transcript.
authors.affiliation: Institute of Reproductive Medicine of the University, Münster, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Gromoll
authors.forename: B
authors.initials: B
authors.lastname: Dankbar
authors.forename: R S
authors.initials: RS
authors.lastname: Sharma
authors.forename: E
authors.initials: E
authors.lastname: Nieschlag
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D006801:Humans; D008252:Macaca fascicularis / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D011061:Poly A / Q000032:analysis / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis / Q000378:metabolism; D012333:RNA, Messenger; D051381:Rats; D011962:Receptors, FSH / Q000096:biosynthesis* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013737:Testis / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D011962:Receptors, FSH; D011061:Poly A; D012313:RNA