Interleukin-5 messenger RNA and immunoreactive protein expression by activated eosinophils in lesional atopic dermatitis skin.
The main cellular sources of interleukin-5 (IL-5) are T lymphocytes and mast cells. Recently, IL-5 mRNA has been identified in eosinophils from patients with celiac disease, eosinophilic heart diseases, and asthma. In an attempt to determine whether IL-5 is generated by eosinophils in atopic dermatitis we have used i) in situ hybridization with 35S-labeled IL-5 RNA probe combined with immunohistochemistry using a monoclonal antibody (MoAb) (EG2) directed against the activated form of Eosinophil Cationic Protein (ECP) and ii) double-immunostaining with anti-IL-5 MoAb and polyclonal anti-ECP antibody. We found that dermal eosinophils from lesional atopic dermatitis skin express IL-5 mRNA and protein. Moreover, highly purified blood eosinophils were also labeled with anti-IL-5 antibodies. The expression of IL-5 by eosinophils in atopic dermatitis might suggest an autocrine pathway of eosinophil differentiation and activation.
Tanaka
Y
Y
Centre d'Immunologie et Biologie Parasitaire, Inserm U167, Institut Pasteur, Lille, France.
Delaporte
E
E
Dubucquoi
S
S
Gounni
A S
AS
Porchet
E
E
Capron
A
A
Capron
M
M
D003593:Cytoplasm / Q000737:chemistry / Q000648:ultrastructure; D003876:Dermatitis, Atopic / Q000097:blood / Q000473:pathology*; D004804:Eosinophils / Q000737:chemistry* / Q000276:immunology / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D015848:Interleukin-5 / Q000235:genetics*; D011506:Proteins / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis*; D012867:Skin / Q000737:chemistry*; D013194:Staining and Labeling
D015848:Interleukin-5; D011506:Proteins; D012333:RNA, Messenger