Direct isolation and expansion of human CD34+ hematopoietic stem cells.
The isolation and expansion of CD34+ cells has numerous applications as supportive therapy for cytopenias that occur postchemotherapy. In this report, we describe a simple system to directly isolate and culture human CD34+ cells. Using this system, CD34+ cells isolated from bone marrow were cultured in the presence of GM-CSF, G-CSF, erythropoietin, stem cell factor, IL-1, IL-3, and IL-6. Cell expansions up to 100-fold were noted during the first 2 weeks of culture with high maintenance of CD34+ cells during the first week of culture. Maximal expansions of progenitors were observed at one week of culture, with, on average, 20-fold increases in CFU-GM number. The results support the feasibility of CD34+ cell expansion for clinical application.
Lebkowski
J
J
Applied Immune Sciences, Santa Clara, CA 95054.
Schain
L
L
Harvey
M
M
Moseley
A
A
Okarma
T B
TB
D015703:Antigens, CD; D018952:Antigens, CD34; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D005240:Feasibility Studies; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D016130:Immunophenotyping; D007163:Immunosorbent Techniques; D007378:Interleukins / Q000494:pharmacology*; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; D010835:Phytohemagglutinins
D015703:Antigens, CD; D018952:Antigens, CD34; D016298:Hematopoietic Cell Growth Factors; D007378:Interleukins; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C028824:soybean lectin; D010835:Phytohemagglutinins