title: Modulation of cell surface-associated mannoprotein antigen expression in experimental candidal vaginitis.
abstract: The monoclonal antibody (MAb) AF1 recognizes an oligosaccharide epitope present on highly immunogenic and immunomodulatory mannoproteins (MP) of Candida albicans. The expression of this epitope (AF1-MP) during experimental candidal vaginitis was studied in two strains of C. albicans (3153 and CA-2) which were equally vaginopathic but differed in the mode of hypha formation in the vagina. In both strains, immunofluorescence of vaginal samples, taken 1 h after challenge, revealed an intense, MAb AF1-specific labelling of the yeast cells. This labelling was very scarce in fungal cells taken at 24 h and on subsequent days during the development of filamentous forms. Electron-microscopic gold immunolabelling observations showed that molecules carrying AF1-MP spanned the entire cell wall in the initial yeast cells but were absent on the cell surface and in the outermost, capsular layer of the cell wall of the germ tubes and filamentous forms. In both strains, at any time and for any form of intravaginal growth, AF1-MP was clearly expressed in the cytoplasm and cytoplasmic vesicles, and was fully incorporated into the inner layers of the cell wall. As seen by immunofluorescence, the vaginal fluid from C. albicans-infected rats did not hinder the expression of AF1-MP on the yeast cells surface in vitro. In electron-microscopic gold immunolabelling, a hypha-specific MAb (3D9) labelled the surface of the hyphal but not of the yeast cells of C. albicans harvested from rat vagina. Overall, these data strongly suggest that cell surface expression of MP antigen is modulated during intravaginal growth and morphogenesis of C. albicans.
authors.affiliation: Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: F
authors.initials: F
authors.lastname: De Bernardis
authors.forename: A
authors.initials: A
authors.lastname: Molinari
authors.forename: M
authors.initials: M
authors.lastname: Boccanera
authors.forename: A
authors.initials: A
authors.lastname: Stringaro
authors.forename: R
authors.initials: R
authors.lastname: Robert
authors.forename: J M
authors.initials: JM
authors.lastname: Senet
authors.forename: G
authors.initials: G
authors.lastname: Arancia
authors.forename: A
authors.initials: A
authors.lastname: Cassone
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal / Q000378:metabolism*; D002176:Candida albicans / Q000254:growth & development / Q000276:immunology* / Q000648:ultrastructure; D002181:Candidiasis, Vulvovaginal / Q000209:etiology / Q000276:immunology* / Q000382:microbiology; D004195:Disease Models, Animal; D000939:Epitopes / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D008562:Membrane Glycoproteins / Q000276:immunology*; D016253:Microscopy, Immunoelectron; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D000946:Antigens, Fungal; D000939:Epitopes; D008562:Membrane Glycoproteins; C025363:mannoproteins