Construction of cDNA libraries from limiting amounts of material.
The use of cDNA libraries has become increasingly widespread as new techniques for library construction and analysis have become available. In recent years, interest in cell-type or stage-specific gene expression has necessitated the construction of cDNA libraries from very small numbers of cells. Recent advances in techniques of RNA isolation, cDNA synthesis, library construction, and the use of the polymerase chain reaction have made this a realistic goal.
McCarrey
J R
JR
Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228.
Williams
S A
SA
D000818:Animals; D001709:Biotechnology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics*; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D008745:Methylation; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics / Q000302:isolation & purification
D018076:DNA, Complementary; D012313:RNA