Antibodies to murine CD40 stimulate normal B lymphocytes but inhibit proliferation of B lymphoma cells.
A rat anti-mouse CD40 antiserum has been prepared by hyperimmunisation of Lewis rats with a highly purified preparation of the recombinant extracellular domain of murine CD40. This antiserum specifically binds CD40-expressing L cell transfectants, but not untransfected L cells, and induces vigorous proliferation of highly purified small dense B cells obtained from the spleens of unstimulated mice. Anti-CD40-induced B cell proliferation can be augmented by the addition of IL-4 and is inhibited by purified recombinant soluble mouse CD40. Interestingly the same anti-CD40 antiserum specifically inhibits the in vitro growth of A.20 murine B lymphoma cells. The specificity of this inhibition can be demonstrated by reversing the effect with purified recombinant soluble mouse CD40. These data implicate CD40 as a possible target for therapeutic intervention in the treatment of B lymphomas.
Heath
A W
AW
DNAX Research Institute, Palo Alto, California 94304.
Chang
R
R
Harada
N
N
Santos-Argumedo
L
L
Gordon
J
J
Hannum
C
C
Campbell
D
D
Shanafelt
A B
AB
Clark
E A
EA
Torres
R
R
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology*; D001483:Base Sequence; D019013:CD40 Antigens; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D005260:Female; D007106:Immune Sera / Q000096:biosynthesis* / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology*; D016393:Lymphoma, B-Cell / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009036:Moths; D051381:Rats; D011917:Rats, Inbred Lew; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D012995:Solubility
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D019013:CD40 Antigens; D007106:Immune Sera; D011994:Recombinant Proteins