title: A simple and inexpensive chromatographic method for the purification of gamma-globulin from human serum.
abstract: Inexpensive cation- and anion-exchangers based on continuous polymer beds were prepared directly in a plastic syringe. These beds are unique in the sense that they are synthesized by a very simple and cost-effective procedure; for instance, no preparation of beads is required as in conventional methods. Highly purified gamma-globulin could be prepared easily by step-wise elution accomplished by forcing buffers of increasing salt concentration through the column with the aid of the plunger.
authors.affiliation: Department of Biochemistry, University of Uppsala, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Mohammad
authors.forename: S
authors.initials: S
authors.lastname: Hjertén
mesh_terms: D002852:Chromatography, Ion Exchange / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D003362:Cost-Benefit Analysis; D006801:Humans; D005719:gamma-Globulins / Q000302:isolation & purification*
chemical_list: D005719:gamma-Globulins