Poor transduction efficiency of human hematopoietic progenitor cells by a high-titer amphotropic retrovirus producer cell clone.
The transduction efficiency of human bone marrow CD34+ cells with supernatants from the retrovirus producer cell clone PA317/LGSN 16 was only one-fifth of that with supernatants from GP+ envAm12/LGSN 15, even though both producers had similar infection titers on 3T3 cells. PA317/LGSN 16-conditioned medium inhibited the proliferation of the bone marrow CD34+ cells, and this inhibitory effect was partially blocked by anti-transforming growth factor beta antibodies. These studies suggest that cytokine secretion plays a role in the suppression of retrovirus transduction of human CD34+ cells.
Xu
L C
LC
Molecular and Medical Genetics Section, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, MD 20892.
Young
H A
HA
Blanco
M
M
Kessler
S
S
Roberts
A B
AB
Karlsson
S
S
D016475:3T3 Cells; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D016207:Cytokines / Q000096:biosynthesis; D018014:Gene Transfer Techniques; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D051379:Mice; D012190:Retroviridae / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000502:physiology
D015703:Antigens, CD; D018952:Antigens, CD34; D016207:Cytokines; D016212:Transforming Growth Factor beta