title: Developmental relationships between hemopoiesis and vasculogenesis.
abstract: Using avian chimeras, we have demonstrated earlier that the stem cells seeding the definitive hemopoietic organs form within the embryo rather than in the yolk sac. We now report experimental evidence indicating that hemopoietic progenitors appear in mouse embryos in the para-aortic splanchnopleura, a location similar to the one that produces stem cells in avian embryos. This structure obtained from E8.5 embryos was grafted under the kidney capsule of adult SCID mice. One compartment of the B lymphoid system became reconstituted with cells derived from the graft, that were identified with genetic and antigenic markers. In vitro data are also in favor of the production of progenitors from this structure. Finally a strategy designed to understand the developmental links between the endothelial network and hemopoietic cells is described. It is based on the expression patterns of two protooncogenes, c-ets1 and c-myb, activated during the amplification period of each of these lineages.
authors.affiliation: Institut d'Embryologie Cellulaire et Moléculaire du CNRS, Collège de France, Nogent-sur-Marne.
authors.forename: F
authors.initials: F
authors.lastname: Dieterlen-Lièvre
authors.forename: L
authors.initials: L
authors.lastname: Pardanaud
authors.forename: I
authors.initials: I
authors.lastname: Godin
authors.forename: J
authors.initials: J
authors.lastname: Garcia-Porrero
authors.forename: A
authors.initials: A
authors.lastname: Cumano
authors.forename: M
authors.initials: M
authors.lastname: Marcos
mesh_terms: D000818:Animals; D002642:Chick Embryo; D006410:Hematopoiesis / Q000235:genetics; D006412:Hematopoietic Stem Cells / Q000166:cytology; D051379:Mice; D016513:Mice, SCID / Q000196:embryology*; D009389:Neovascularization, Pathologic; D020626:Oncogene Proteins v-myb; D015682:Retroviridae Proteins, Oncogenic / Q000378:metabolism; D013152:Splanchnic Circulation
chemical_list: D020626:Oncogene Proteins v-myb; D015682:Retroviridae Proteins, Oncogenic; C050379:oncogene proteins v-ets