Correlation between esterase electrophoretic polymorphism and virulence-associated traits in extra-intestinal invasive strains of Escherichia coli.
The electrophoretic variations of carboxylesterase B and of esterases A, C and I, the presence of mannose resistant haemagglutinin, alpha-haemolysin, cytotoxic necrotizing factor type 1 (CNF1) and certain O antigens were compared in 150 strains of Escherichia coli responsible for extra-intestinal infections. Electrophoretic mobilities of outer membrane proteins (OMP) were also studied for strains belonging to O4, O6, O7, O8 and O75 serogroups. Fast migrating allozymes of carboxylesterase B (pattern B1) were correlated with slow migrating allozymes of esterase C, serogroups O7 and O8, lack of virulence factor, and particular OMP patterns, whereas slow migrating allozymes of carboxylesterase B (pattern B2) were correlated with fast migrating allozymes of esterase C, serogroups O2, O4, O6, O18 and O75, virulence factor production, and distinct OMP patterns. Allozymes of esterases A and I were not clearly correlated with the distribution of virulence factors. The pattern B2 was more strongly associated with CNF1 than with alpha-haemolysin and mannose resistant haemagglutinin. These results substantiate the view that the electrophoretic pattern B2 of carboxylesterase B identified most of the highly pathogenic strains implicated in extra-intestinal infection of humans.
Goullet
P
P
Laboratoire de Microbiologie, Faculté de Médecine Xavier Bichat, Paris, France.
Picard
B
B
Contrepois
M
M
De Rycke
J
J
Barnouin
J
J
D016470:Bacteremia / Q000382:microbiology; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis; D001427:Bacterial Toxins / Q000032:analysis; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D003603:Cytotoxins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins; D004950:Esterases / Q000032:analysis*; D006388:Hemagglutinins / Q000032:analysis; D006460:Hemolysin Proteins / Q000032:analysis; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D019081:O Antigens; D011110:Polymorphism, Genetic; D011135:Polysaccharides, Bacterial / Q000032:analysis; D012703:Serotyping; D014552:Urinary Tract Infections / Q000382:microbiology; D014774:Virulence
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003603:Cytotoxins; D029968:Escherichia coli Proteins; D006388:Hemagglutinins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D007527:Isoenzymes; D019081:O Antigens; D011135:Polysaccharides, Bacterial; C060296:cytotoxic necrotizing factor type 1; D004950:Esterases; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase