title: Isolation and characterization of phosphoprotein phosphatase 1 from alfalfa.
abstract: Protein phosphatases are central regulatory components of diverse processes in eukaryotes and are among the most highly conserved proteins known. In this paper, we report the cloning and sequencing of a type 1 protein phosphatase (pp1Ms) cDNA from alfalfa. Southern analysis indicates the presence of a gene family of PP1 proteins in alfalfa. The pp1Ms open reading frame is very similar to one of five predicted Arabidopsis type 1 protein phosphatases, indicating that different subtypes are individually conserved. Expression of the alfalfa pp1Ms in a temperature-sensitive Schizosaccharomyces pombe PP1 mutant, dis2-11, revealed no complementation, suggesting that PP1Ms is not involved in mitotic regulation. In different plant organs, different pp1Ms transcript levels were observed; in contrast, mRNA levels remained constant in all phases of the cell cycle and in logarithmically growing cells. However, when cells entered stationary phase pp1Ms transcript levels decreased considerably.
authors.affiliation: Institute of Microbiology and Genetics, Biocenter, Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Pay
authors.forename: M
authors.initials: M
authors.lastname: Pirck
authors.forename: L
authors.initials: L
authors.lastname: Bögre
authors.forename: H
authors.initials: H
authors.lastname: Hirt
authors.forename: E
authors.initials: E
authors.lastname: Heberle-Bors
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002455:Cell Division; D002478:Cells, Cultured; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D004330:Drosophila / Q000235:genetics; D005434:Flow Cytometry; D015870:Gene Expression; D000455:Medicago sativa / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D010944:Plants / Q000201:enzymology / Q000235:genetics; D011061:Poly A / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D054645:Protein Phosphatase 1; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D012333:RNA, Messenger; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1