Improvement of chromosomal DNA extraction from different yeast species by analysis of single preparation steps.
A modified procedure is proposed for chromosomal DNA extraction based on a cell-wall lytic enzyme never applied before in pulsed field gel electrophoresis. Protoplasting efficiency is retained under very challenging conditions for enzyme activity, such as those required for non-Saccharomyces yeasts often characterized by cell walls highly resistant to lysis.
Cardinali
G
G
Dipartimento di Biologia Vegetale, University of Perugia, Italy.
Pellegrini
L
L
Martini
A
A
D002473:Cell Wall / Q000378:metabolism; D015825:Chromosomes, Fungal; D004271:DNA, Fungal / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005658:Fungi / Q000737:chemistry; D007621:Karyotyping / Q000379:methods; D012232:Rhizoctonia / Q000201:enzymology
D004271:DNA, Fungal