Endothelin receptor density in human hypertrophic and non-hypertrophic prostate tissue.
The amount of endothelin receptors in human prostate tissue was measured by radioligand binding techniques using 125I-Endothelin -1 and -3 (125I-ET-1, -3). Specimens of the non-hypertrophy group were obtained from 6 patients who underwent total cystectomy under the diagnosis of bladder cancer and those of the hypertrophy group from 6 prostatic hypertrophy patients who underwent open prostatectomy. 125I-ET-1 bound to the prostate tissue with the KD value of 0.033 +/- 0.012 nM in the non-hypertrophy group and with the KD value of 0.035 +/- 0.012 nM in the hypertrophy group. 125I-ET-3 bound to the prostate tissue with the KD value of 0.023 +/- 0.011 nM in the non-hypertrophy group and with the KD value of 0.029 +/- 0.016 nM in the hypertrophy group. The KD values were not significantly different between the hypertrophy and non-hypertrophy groups. The KD values of 125I-ET-1 and 125I-ET-3 were similar. The Bmax values (fmol/mg protein) of 125I-ET-1 binding to the prostate tissue were 32.18 +/- 3.69 to the non-hypertrophy group and 85.66 +/- 20.65 to the hypertrophy group. The Bmax values (fmol/mg protein) of 125I-ET-3 binding to the prostate tissue were 27.48 +/- 5.25 to the non-hypertrophy group and 75.90 +/- 13.46 to the hypertrophy group. The Bmax values of both 125I-ET-1 and 125I-ET-3 were significantly higher in the hypertrophy group than in the non-hypertrophy group.(ABSTRACT TRUNCATED AT 250 WORDS)
Kondo
S
S
Second Department of Biochemistry Akita University, School of Medicine.
Morita
T
T
Tashima
Y
Y
D015653:Cystectomy; D016232:Endothelins / Q000378:metabolism; D006801:Humans; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011468:Prostatectomy; D011470:Prostatic Hyperplasia / Q000378:metabolism* / Q000601:surgery; D011869:Radioligand Assay; D017466:Receptors, Endothelin / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000601:surgery
D016232:Endothelins; D017466:Receptors, Endothelin