title: Polymorphonuclear neutrophils release 35S-labelled proteoglycans into cartilage during frustrated phagocytosis.
abstract: Rabbit peritoneal polymorphonuclear neutrophils (PMN), incubated in medium containing [35S]sulphate, incorporated 35S into proteoglycan and protein fractions. Approximately 46% of the 35S-labelled macromolecules associated with the PMN cells after 1 h of incubation were recovered in a cytoplasmic granule extract, the majority being present in azurophil granules. Analysis of the azurophil granule fraction showed that approximately 90% of the 35S-labelled macromolecules were proteoglycans. When challenged with heat-aggregated rabbit gamma-globulin in the presence of cytochalasin B and cGMP, PMN were induced to release granular enzymes but did not release 35S-labelled proteoglycans into the incubation medium. When incubated with articular cartilage slices, PMN released their granule 35S-labelled proteoglycan into the medium and into the cartilage matrix. Granule enzymes and 35S-labelled granule proteoglycan were extracted from the cartilage tissue after incubation and 35S-labelled macromolecules were detected in the cartilage tissue by autoradiography.
authors.affiliation: Department of Biochemistry, Monash University, Clayton, Australia.
authors.forename: E E
authors.initials: EE
authors.lastname: Gardiner
authors.forename: S S
authors.initials: SS
authors.lastname: Mok
authors.forename: A
authors.initials: A
authors.lastname: Sriratana
authors.forename: H C
authors.initials: HC
authors.lastname: Robinson
authors.forename: B J
authors.initials: BJ
authors.lastname: Veitch
authors.forename: D A
authors.initials: DA
authors.lastname: Lowther
authors.forename: C J
authors.initials: CJ
authors.lastname: Handley
mesh_terms: D000818:Animals; D001345:Autoradiography; D002358:Cartilage, Articular / Q000378:metabolism*; D015550:Cell Degranulation; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000201:enzymology; D005966:Glucuronidase / Q000378:metabolism*; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism*; D010587:Phagocytosis; D011509:Proteoglycans / Q000378:metabolism*; D011817:Rabbits; D005719:gamma-Globulins / Q000378:metabolism
chemical_list: D011509:Proteoglycans; D005719:gamma-Globulins; D005966:Glucuronidase