An effective strategy for decontamination, ex vivo expansion, and storage of human fetal liver hematopoietic stem cells.
The transplantation of human fetal tissue has the potential to cure a variety of life-threatening diseases. The strategy for procurement, quality control, and functional assessment of human fetal liver HSC may prove useful for the transplantation of other fetal tissues. In addition to technical limitations, there are ethical and legal issues which need to be resolved before widespread use of fetal tissue. Further development of regulatory standards for the acquisition and distribution of fetal tissues will foster the application of this novel technology.
Rice
H E
HE
Fetal Treatment Center, University of California, San Francisco 94143.
Skarsgard
E D
ED
Emani
V R
VR
Zanjani
E D
ED
Harrison
M R
MR
Flake
A W
AW
D000028:Abortion, Induced; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D015815:Cell Adhesion Molecules; D003470:Culture Media; D003666:Decontamination / Q000379:methods; D005260:Female; D005333:Fetus; D016298:Hematopoietic Cell Growth Factors; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007258:Informed Consent; D007377:Interleukin-3; D008099:Liver / Q000166:cytology* / Q000196:embryology; D009077:Mucins / Q000032:analysis; D011247:Pregnancy; D012756:Sheep; D019089:Stem Cell Factor; D014021:Tissue Preservation / Q000379:methods*; D014183:Transplantation, Heterologous
D015703:Antigens, CD; D018952:Antigens, CD34; D015815:Cell Adhesion Molecules; D003470:Culture Media; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D009077:Mucins; D019089:Stem Cell Factor