The recognition of E. coli glutamine tRNA by glutaminyl-tRNA synthetase.
A variety of genetic, biochemical and structural studies have been used to determine factors ensuring the accuracy of recognition by aminoacyl-tRNA synthetases for tRNA. The identity elements of Escherichia coli tRNA(Gln) are located mainly in the anticodon and acceptor stem, and ensure the accurate recognition of the tRNA by glutaminyl-tRNA synthetase. We summarize a number of experimental techniques to define the accuracy of aminoacylation in vivo and in vitro.
Rogers
M J
MJ
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
Weygand-Durasević
I
I
Schwob
E
E
Sherman
J M
JM
Rogers
K C
KC
Thomann
H U
HU
Sylvers
L A
LA
Ohtsuka
E
E
Inokuchi
H
H
Söll
D
D
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000926:Anticodon; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism*; D012352:RNA, Transfer, Glu / Q000737:chemistry / Q000378:metabolism*
D000926:Anticodon; D012329:RNA, Bacterial; D012352:RNA, Transfer, Glu; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase