title: Mapping of the genes encoding human inducible and endothelial nitric oxide synthase (NOS2 and NOS3) to the pericentric region of chromosome 17 and to chromosome 7, respectively.
abstract: Nitric oxide (NO) is an important molecular messenger regulating the functions of a wide variety of cells and tissues. NO is synthesized from L-arginine by a variety of isoforms of the enzyme nitric oxide synthase (NOS). We have used Southern blotting analysis on DNAs obtained from a panel of human-rodent hybrid cell lines to map the gene encoding the inducible NOS (NOS2) to chromosome 17cen-17q11 and the gene encoding the endothelial form of NOS (NOS3) to chromosome 7. Fluorescence in situ hybridization using a NOS2 probe gave several signals in the 17p11-q11 pericentromeric region.
authors.affiliation: Department of Neurobiology, AFRC, Babraham Institute, Cambrige, United Kingdom.
authors.forename: W
authors.initials: W
authors.lastname: Xu
authors.forename: I G
authors.initials: IG
authors.lastname: Charles
authors.forename: S
authors.initials: S
authors.lastname: Moncada
authors.forename: P
authors.initials: P
authors.lastname: Gorman
authors.forename: D
authors.initials: D
authors.lastname: Sheer
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: P
authors.initials: P
authors.lastname: Emson
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002358:Cartilage, Articular / Q000201:enzymology; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D002897:Chromosomes, Human, Pair 7; D003360:Cosmids; D017931:DNA Primers; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells; D007527:Isoenzymes / Q000235:genetics; D008969:Molecular Sequence Data; D019001:Nitric Oxide Synthase; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers; D007527:Isoenzymes; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases