Functional and phenotypic assessment of neonatal human leucocytes expressing natural killer cell-associated antigens.
A subpopulation of mononuclear leucocytes was prepared from umbilical cord venous blood by immunomagnetic depletion of lymphocytes and monocytes using monoclonal antibodies to CD2, CD3, CD14 and CD19 antigens, and examined for NK cell-associated phenotypic and functional properties. The depleted population was enriched for the NK markers CD16 (mean 53.6% positive) and CD56 (mean 42.7% positive). While there was considerable overlap of these two markers, approximately one-third of CD16+ cells were CD56-; in contrast, few CD56+ CD16- cells were found. CD16+/CD56+ cells also co-expressed CD7 and CD45RA antigens, while a minority weakly expressed CD8. Another marker of adult NK cells, CD57, was virtually absent from CD16+/CD56+ cells, as was MHC Class 2. Freshly depleted cord cells had virtually absent natural cytotoxicity to K562 targets in a chromium release assay, but NK activity could be induced after 18 h exposure to recombinant human IL-2, without significant change in phenotype. These findings confirm the phenotypic differences and functional defects of NK cells in cord blood as compared to adult blood, and identify a subset of cells with unique phenotype (CD2- CD3- CD7+ CD16+ CD56- CD57-). The precise relationship of this subset of cells to NK lineage remains to be defined.
Bradstock
K F
KF
Department of Haematology, Westmead Hospital, New South Wales, Australia.
Luxford
C
C
Grimsley
P G
PG
D000328:Adult; D015703:Antigens, CD / Q000097:blood*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000097:blood; D019002:CD56 Antigen; D003601:Cytotoxicity Tests, Immunologic; D005312:Fetal Blood / Q000166:cytology / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000097:blood; D006801:Humans; D018189:Immunomagnetic Separation; D016130:Immunophenotyping; D007231:Infant, Newborn; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D017452:Receptors, IgG / Q000032:analysis
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D019002:CD56 Antigen; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D017452:Receptors, IgG