title: Transplantation of CD34+ hematopoietic progenitor cells.
abstract: Sixty-six stage IV breast cancer patients received high dose chemotherapy followed by autologous transplantation of CD34-positive(+) cells obtained from the bone marrow and/or granulocyte colony stimulating factor (G-CSF)-mobilized peripheral blood. Grafts were examined for the presence of tumor using conventional histology and immunocytochemical staining. Patients achieved a granulocyte count of 500 x 10(9)/liter 10-12 days posttransplant, with a platelet count of > 20 x 10(9)/liter in 14-15 days. Enrichment of CD34+ cells from the peripheral blood progenitor cell (PBPC) collections resulted in a 1.3 to 4.0 log depletion of breast cancer cells from the graft.
authors.affiliation: Bone Marrow Transplant Program, University of Colorado, Denver.
authors.forename: E J
authors.initials: EJ
authors.lastname: Shpall
authors.forename: R B
authors.initials: RB
authors.lastname: Jones
authors.forename: S I
authors.initials: SI
authors.lastname: Bearman
authors.forename: M H
authors.initials: MH
authors.lastname: Purdy
authors.forename: W A
authors.initials: WA
authors.lastname: Franklin
authors.forename: S
authors.initials: S
authors.lastname: Heimfeld
authors.forename: R J
authors.initials: RJ
authors.lastname: Berenson
mesh_terms: D015703:Antigens, CD / Q000097:blood*; D018952:Antigens, CD34; D001943:Breast Neoplasms / Q000628:therapy*; D002469:Cell Separation; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34