title: A sialyl-Le(x)-negative melanoma cell line binds to E-selectin but not to P-selectin.
abstract: The adhesion molecules E-selectin (ELAM-1) and P-selectin (GMP-140/CD62) recognize the carbohydrate motives sialyl-Le(x), sialyl-diLe(x), or sialyl-Lea, though with different affinity. We found that the melanoma cell line NKI-4 bound to E-selectin, but not to P-selectin. This melanoma cell line did not express sialyl-Le(x), but was positive for sialyl-diLe(x) and sialyl-Le(a). In contrast, 2 other melanoma cell lines, MeWo and SK-MEL-28, expressing either sialyl-diLe(x) or sialyl-Le(a) on the cell surface, bound neither E-selectin nor P-selectin. Transfection of the fucosyltransferases Fuc-TIII, Fuc-TIV, and Fuc-TV mediates cell surface expression of sialyl-Le(x) in many cell lines. We detected transcripts of the fucosyltransferases Fuc-TIII and Fuc-TV in 4 melanoma cell lines despite the absence of cell surface sialyl-Le(x). Our observations indicate that expression of fucosyltransferases (Fuc-TIII and -TV) and generation of cell-surface sialyl-diLe(x) are not sufficient to permit adherence to E-selectin or P-selectin. Furthermore, it seems possible that a yet undefined ligand different from sialyl-Le(x), sialyl-diLe(x), or sialyl-Le(a) enables melanoma cells to adhere to E-selectin.
authors.affiliation: Department of Internal Medicine, Universitätsklinikum Steglitz, Berlin, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Kunzendorf
authors.forename: S
authors.initials: S
authors.lastname: Krüger-Krasagakes
authors.forename: M
authors.initials: M
authors.lastname: Notter
authors.forename: H
authors.initials: H
authors.lastname: Hock
authors.forename: G
authors.initials: G
authors.lastname: Walz
authors.forename: T
authors.initials: T
authors.lastname: Diamantstein
mesh_terms: D001483:Base Sequence; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D019040:E-Selectin; D005434:Flow Cytometry; D005647:Fucosyltransferases / Q000235:genetics; D006801:Humans; D016256:Lewis X Antigen / Q000032:analysis*; D008545:Melanoma / Q000378:metabolism* / Q000473:pathology; D008969:Molecular Sequence Data; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D015815:Cell Adhesion Molecules; D019040:E-Selectin; D016256:Lewis X Antigen; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D005647:Fucosyltransferases