title: Evaluation of integrated morpho-biological indicators in breast cancer.
abstract: A series of 71 patients undergoing surgery for primary breast carcinoma was prospectively studied in order to verify the relationships between clinical and pathological variables, proliferative indexes and DNA-ploidy. Significant correlations between proliferative indexes were found; conversely, DNA-ploidy showed no correlation with either PCNA/cyclin or AgNORs, but only with flow cytometry S-phase fraction (FCM-S) and IF-vimentin. In the 42 patients with a mean follow-up of 42 months, disease-free rate was best predicted by nuclear grade (p < 0.001), histological grade (p < 0.001), mitoses (p 0.001), IF-vimentin (p 0.002), FCM-S (p 0.002), PCNA (p 0.005) and Ag-NORs (p 0.007).
authors.affiliation: Cattedra di Anatomia ed Istologia Patologica R, University of Palermo, Italy.
authors.forename: R M
authors.initials: RM
authors.lastname: Tomasino
authors.forename: A
authors.initials: A
authors.lastname: Russo
authors.forename: V
authors.initials: V
authors.lastname: Bazan
authors.forename: V
authors.initials: V
authors.lastname: Morello
authors.forename: V
authors.initials: V
authors.lastname: Tralongo
authors.forename: C
authors.initials: C
authors.lastname: Nagar
authors.forename: M
authors.initials: M
authors.lastname: Salvato
authors.forename: P
authors.initials: P
authors.lastname: Taormina
authors.forename: A
authors.initials: A
authors.lastname: Marrazzo
authors.forename: R
authors.initials: R
authors.lastname: Nuara
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D001943:Breast Neoplasms / Q000175:diagnosis*; D004273:DNA, Neoplasm / Q000032:analysis; D006360:Heat-Shock Proteins; D006801:Humans; D007633:Keratins / Q000032:analysis; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D010750:Phosphoproteins / Q000032:analysis; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen; D011446:Prospective Studies; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D016196:S Phase; D014746:Vimentin / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D006360:Heat-Shock Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D018809:Proliferating Cell Nuclear Antigen; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D014746:Vimentin; D007633:Keratins