Functional and phenotypical analysis of subsets of rat CD4+ T cells.
Rat CD4+ T cells were divided into two distinct subsets by a monoclonal antibody RTH-1 recognizing a unique epitope on rat CD45R. Cellular distribution of OX-22- and RTH-1-defined antigens was the same. However, OX-22 and RTH-1 recognized different epitopes that exist on rat CD45R. The expression of IL-4 gene was detected only in RTH-1low CD4+ T cell subset upon various stimulations. In contrast, the expression of IL-2 and IFN-gamma gene varied depending upon the nature of stimuli. The increased cell surface expression of CD44 was detected in RTH-1high CD4+ T cell subset. Conversely the increased expression of CD2 was detected in RTH-1low CD4+ T cell subset. The expression of CD3 and LFA-1 was not significantly different between RTH-1high and RTH-1low subsets.
Nagai
Y
Y
Section of Immunopathogenesis, Hokkaido University, Japan.
Inobe
M
M
Kikuchi
K
K
Uede
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000502:physiology; D015870:Gene Expression; D018960:Hyaluronan Receptors; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D017493:Leukocyte Common Antigens / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008969:Molecular Sequence Data; D051381:Rats / Q000276:immunology*; D011916:Rats, Inbred F344 / Q000276:immunology; D011917:Rats, Inbred Lew / Q000276:immunology; D016201:Receptors, Lymphocyte Homing / Q000032:analysis
D000911:Antibodies, Monoclonal; D018960:Hyaluronan Receptors; D007376:Interleukin-2; D016201:Receptors, Lymphocyte Homing; D015847:Interleukin-4; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens