title: The structure of the O-specific polysaccharide of Hafnia alvei strain 1216.
abstract: The O-specific polysaccharide of Hafnia alvei strain 1216 is composed of D-galactose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, 3,6-dideoxy-3-[(R)-3-hydroxybutyramido]-D-glucose, and O-acetyl groups in the ratios 1:1:2:1:1. On the basis of sugar and methylation analyses of the intact and chemically degraded (O-deacetylated, carboxyl-reduced, Smith-degraded) polysaccharide and 1H and 13C NMR spectroscopy, including 2D shift-correlated (COSY, relayed COSY, 13C, 1H-COSY) and 1D NOE spectroscopy, it was concluded that the O-antigen is built up of linear pentasaccharide units having the following structure: [formula: see text]
authors.affiliation: L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw.
authors.forename: E
authors.initials: E
authors.lastname: Katzenellenbogen
authors.forename: E
authors.initials: E
authors.lastname: Romanowska
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: N A
authors.initials: NA
authors.lastname: Kocharova
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: N K
authors.initials: NK
authors.lastname: Kochetkov
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D004755:Enterobacteriaceae / Q000737:chemistry* / Q000276:immunology; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D002247:Carbon Isotopes; D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D009005:Monosaccharides; D019081:O Antigens; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial