Effect of sera of cirrhotic patients with or without hepatitis B virus infection on protein synthesis in hepatoma cells.
The in vitro effects of sera of 11 patients with liver cirrhosis on protein synthesis in PLC/PRF/5 cells were studied. Hepatitis B virus (HBV) infection was documented in 7 patients. Increased random production of several cell proteins of M(r) of approximately 25, 65, 90 and 130 K was shown by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). There was no correlation between HBV-positive and HBV-negative cirrhosis and the induced proteins. One of them was identified as alpha-1 foetoprotein by immunoblot analysis. C-reactive protein (CRP) was determined only in one case; production of interleukin-6 (IL-6) was not detected.
Hajnická
V
V
Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republik.
Volná
A
A
Stancek
D
D
Oltman
M
M
D000293:Adolescent; D000328:Adult; D002097:C-Reactive Protein / Q000096:biosynthesis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D005260:Female; D006509:Hepatitis B / Q000097:blood / Q000276:immunology*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D008103:Liver Cirrhosis / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D014176:Protein Biosynthesis; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000096:biosynthesis
D015850:Interleukin-6; D000509:alpha-Fetoproteins; D002097:C-Reactive Protein