Characterization of the muscarine receptor subtype on sympathetic nerve endings in the rat caudal artery.
The prejunctional muscarine receptor on sympathetic nerves in the rat caudal artery was characterized using several selective antagonists. The inhibitory response of carbachol on vasoconstriction elicited by sympathetic nerve stimulation was antagonized by benzhexol (trihexyphenidyl; pKB 7.1), heptane-1,7-bis(dimethyl-3'-phthalimidopropyl ammonium bromide) (C7/3-phth; pKB 6.5) and hexahydrosiladifenidol (HHSiD; apparent pKB 6.0). These pKB values suggest that the receptor most closely resembles the muscarine M2 receptor subtype rather than the muscarine M1, M3 or M4 receptor subtypes.
Shen
A
A
School of Pharmacology, Victorian College of Pharmacy (Monash University), Parkville, Australia.
Mitchelson
F
F
D008455:(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride / Q000494:pharmacology; D000818:Animals; D001158:Arteries / Q000187:drug effects / Q000294:innervation; D001285:Atropine / Q000494:pharmacology; D008725:Bis-Trimethylammonium Compounds / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D054833:Isoindoles; D008297:Male; D018727:Muscarinic Antagonists; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000294:innervation*; D009411:Nerve Endings / Q000187:drug effects*; D010276:Parasympatholytics / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D011976:Receptors, Muscarinic / Q000187:drug effects*; D013564:Sympathetic Nervous System / Q000187:drug effects*; D013623:Tail / Q000098:blood supply*; D014282:Trihexyphenidyl / Q000494:pharmacology
D008725:Bis-Trimethylammonium Compounds; D054833:Isoindoles; D018727:Muscarinic Antagonists; D010276:Parasympatholytics; D010880:Piperidines; D011976:Receptors, Muscarinic; C011268:heptane-1,7-bis(dimethyl-3'-phthalimidopropylammonium); D008455:(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride; D014282:Trihexyphenidyl; D001285:Atropine; D002217:Carbachol; C046773:hexahydrosiladifenidol