Uptake of nitric oxide synthase inhibitors by macrophage RAW 264.7 cells.
Uptake of the nitric oxide synthase inhibitors NG-methyl-L-arginine (L-NMA) and NG-nitro-L-arginine (L-NNA) by macrophages is mediated by two different mechanisms. Activation of the cells with cytokines resulted in an up-regulation of L-NMA uptake but did not affect L-NNA transport. Characterization of the transport sites revealed that uptake of L-NMA is mediated by a cationic amino acid transporter (system y+) whereas a neutral amino acid transporter (system L) accounts for the uptake of L-NNA.
Schmidt
K
K
Institut für Pharmakologie und Toxikologie, Karl-Franzens-Universität Graz, Austria.
Klatt
P
P
Mayer
B
B
D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors*; D026905:Amino Acid Transport Systems; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000378:metabolism; D001692:Biological Transport; D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism; D002460:Cell Line; D007371:Interferon-gamma / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D019001:Nitric Oxide Synthase; D019335:Nitroarginine; D011994:Recombinant Proteins / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D019323:omega-N-Methylarginine
D026905:Amino Acid Transport Systems; D002352:Carrier Proteins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D019335:Nitroarginine; D019323:omega-N-Methylarginine; D007371:Interferon-gamma; D001120:Arginine; D012964:Sodium; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D007930:Leucine