The purine 2-amino group as a critical recognition element for binding of small molecules to DNA.
The expedient of preparing homologous DNA samples substituted with I for G, DAP for A, or both, has been used to investigate the role of the purine 2-amino group in determining the preferred binding sites for antibiotics on DNA. The selectivity of echinomycin for CpG steps, of actinomycin for GpC steps, and of netropsin for A + T-rich tracts, is seen to be radically altered in the substituted DNA molecules.
Waring
M J
MJ
Department of Pharmacology, University of Cambridge, UK.
Bailly
C
C
D015075:2-Aminopurine / Q000031:analogs & derivatives* / Q000378:metabolism; D000225:Adenine / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D001345:Autoradiography; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D003609:Dactinomycin / Q000378:metabolism; D003850:Deoxyribonuclease I; D004448:Echinomycin / Q000378:metabolism; D006147:Guanine / Q000378:metabolism; D007288:Inosine / Q000378:metabolism; D008969:Molecular Sequence Data; D009429:Netropsin / Q000378:metabolism; D009690:Nucleic Acid Conformation; D012365:RNA, Transfer, Tyr / Q000235:genetics
D000900:Anti-Bacterial Agents; D012365:RNA, Transfer, Tyr; D003609:Dactinomycin; D015075:2-Aminopurine; C007300:2,6-diaminopurine; D004448:Echinomycin; D007288:Inosine; D006147:Guanine; D009429:Netropsin; D004247:DNA; D003850:Deoxyribonuclease I; D000225:Adenine