Correlation of the insulin receptor substrate-1 with insulin-responsive deoxyglucose transport in 3T3-L1 adipocytes.
We have devised procedures for decreasing the amount of IRS-1 in 3T3-L1 adipocytes (viz., chronic treatments with insulin, dexamethasone, 1-methyl-3-isobutylxanthine, cycloheximide or actinomycin D) and have determined the correlation between the amounts of IRS-1, insulin receptor, GluT4 and phosphatidylinositol 3'-kinase regulatory subunit with insulin-responsive dGlc transport. Each of these treatments decreased insulin responsiveness that correlated with the amount of IRS-1, but not with the amount of the other signaling proteins or tyrosine-phosphorylated IRS-1. Removal of insulin after chronic treatment resulted in a return of both insulin responsiveness and IRS-1. Increased expression of IRS-1 occurred during differentiation simultaneously with increased insulin-responsive dGlc transport. These data are consistent with a role of IRS-1 in insulin signaling to the glucose transport system.
Rice
K M
KM
Department of Biochemistry and Molecular Biology, Texas Tech University Health Sciences Center, Lubbock 79430.
Garner
C W
CW
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002454:Cell Differentiation; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D003907:Dexamethasone / Q000494:pharmacology; D005347:Fibroblasts / Q000502:physiology; D051275:Glucose Transporter Type 4; D007328:Insulin / Q000378:metabolism / Q000494:pharmacology*; D055504:Insulin Receptor Substrate Proteins; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D009124:Muscle Proteins; D019869:Phosphatidylinositol 3-Kinases; D010750:Phosphoproteins / Q000172:deficiency*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D011972:Receptor, Insulin / Q000378:metabolism; D015398:Signal Transduction
D051275:Glucose Transporter Type 4; D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; C526295:Irs1 protein, mouse; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D010750:Phosphoproteins; C495693:Slc2a4 protein, mouse; D003609:Dactinomycin; D003907:Dexamethasone; D003513:Cycloheximide; D003847:Deoxyglucose; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); D011972:Receptor, Insulin; D015056:1-Methyl-3-isobutylxanthine