cDNA and deduced amino acid sequence of a dwarf goat liver cytochrome P450 fragment belonging to the CYP2C gene subfamily.
From a liver cDNA library derived from a phenobarbital treated dwarf goat a 1176 bp cDNA-fragment, coding for 285 amino acids, has been isolated. Northern blot analysis reveals detection of a 2 kb mRNA which is inducible by phenobarbital, triacetyloleandomycin and to an lesser extent by beta-naphthoflavone. Analysis of the deduced amino acid sequence shows a 72.5% and a 71.1% homology with rabbit CYP2C3 and human CYP2C17, respectively. The nucleotide sequence reveals homologies of 79.8% and 78.7%, respectively. Comparison of the amino acid sequences between CYP2C forms of various species reveals a high homology around the Cys436, the so-called C-terminal cysteine containing peptide, which is assigned to be the heme-binding region.
Zeilmaker
W M
WM
Department of Veterinary Basic Sciences, Utrecht University, The Netherlands.
van 't Klooster
G A
GA
Gremmels-Gehrmann
J F
JF
van Miert
A S
AS
Horbach
G J
GJ
D000595:Amino Acid Sequence; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D015152:Blotting, Northern; D003545:Cysteine; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis / Q000378:metabolism; D006041:Goats; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010634:Phenobarbital / Q000494:pharmacology; D012313:RNA / Q000302:isolation & purification; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D039181:Steroid 16-alpha-Hydroxylase; D013250:Steroid Hydroxylases / Q000096:biosynthesis / Q000235:genetics*
D018076:DNA, Complementary; D012313:RNA; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; D039181:Steroid 16-alpha-Hydroxylase; D003545:Cysteine; D010634:Phenobarbital