Detection of HCV infection by cPCR in patients with acute leukemia.
HCV RNA and anti-HCV were detected respectively by complemented DNA polymerases chain reaction (cPCR) and ELISA in the sera of 28 acute leukemia patients with repeated blood transfusion and changes in liver function. HCV RNA positive rate was 78.6%. Anti-HCV positive rate was 60.7%. 25 subjects showed positive results in HCV RNA or anti-HCV, or in both of them. By combined assessment, the HCV infection rate was 89.3%. Acute leukemia patients were the high risk group of HCV infection because of the lowered immune function and repeated blood transfusion. By cPCR, HCV RNA can be detected earlier and the sensitivity is higher than by anti-HCV. So cPCR is a sensitive and specific method for early diagnosis of HCV infection. The combination of HCV RNA and anti-HCV detection methods may improve the diagnostic rate of HCV infection.
Yao
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Department of Hematology, First Teaching Hospital, Norman Bethune University of Medical Sciences, Changchun.
Fu
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Xiao
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Dong
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Yi
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YL
D000293:Adolescent; D000328:Adult; D005260:Female; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology; D006508:Hepatitis Antibodies / Q000097:blood; D006526:Hepatitis C / Q000175:diagnosis* / Q000635:transmission; D018937:Hepatitis C Antibodies; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000628:therapy*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000628:therapy; D012367:RNA, Viral / Q000097:blood*; D065227:Transfusion Reaction
D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D012367:RNA, Viral