title: Transmissibility of Pseudomonas cepacia infection in clinic patients and lung-transplant recipients with cystic fibrosis.
abstract: BACKGROUND
  In patients with cystic fibrosis, infection with Pseudomonas cepacia is associated with poor outcomes. However, the extent of person-to-person transmission and the source of P. cepacia infection after lung transplantation are not well defined. Using DNA-based typing systems, we sought to determine the genetic relatedness of P. cepacia infection at one cystic fibrosis center.
  
  
  METHODS
  We analyzed 65 P. cepacia isolates gathered over a period of eight years at a single cystic fibrosis center from 17 clinic patients and from 5 patients who underwent double-lung transplantation. The isolates were analyzed by ribotyping and chromosomal fingerprinting based on pulsed-field gel electrophoresis.
  
  
  RESULTS
  Analyses of serial isolates revealed that each clinic patient and transplant recipient harbored a different P. cepacia clone that was persistent. In the transplant recipients, the preoperative and postoperative isolates were identical. In the two patients with disseminated infection after lung transplantation, isolates from multiple sites were identical and indicated clonal expansion of the previous respiratory P. cepacia strain. Pulsed-field gel electrophoresis proved both more discriminative and more practical than ribotyping as a means of defining the genetic relatedness of the P. cepacia isolates.
  
  
  CONCLUSIONS
  Our serial analyses in patients with cystic fibrosis at one center found distinct strains of P. cepacia persistently infecting each patient and no evidence of person-to-person transmission of this organism. P. cepacia infection after lung transplantation was due to the persistence of the strain present before transplantation.
authors.affiliation: Department of Pediatrics, Maxwell Finland Laboratory for Infectious Diseases, Boston, MA 02118.
authors.forename: S
authors.initials: S
authors.lastname: Steinbach
authors.forename: L
authors.initials: L
authors.lastname: Sun
authors.forename: R Z
authors.initials: RZ
authors.lastname: Jiang
authors.forename: P
authors.initials: P
authors.lastname: Flume
authors.forename: P
authors.initials: P
authors.lastname: Gilligan
authors.forename: T M
authors.initials: TM
authors.lastname: Egan
authors.forename: R
authors.initials: R
authors.lastname: Goldstein
mesh_terms: D015373:Bacterial Typing Techniques; D016956:Burkholderia cepacia / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003550:Cystic Fibrosis / Q000382:microbiology* / Q000601:surgery; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008171:Lung Diseases / Q000382:microbiology*; D016040:Lung Transplantation / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D011552:Pseudomonas Infections / Q000382:microbiology / Q000635:transmission*; D012407:rRNA Operon
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal