1H NMR determination of lactate 13C-enrichment in skeletal muscle: using a double quantum filter for the simultaneous editing of 13C-coupled and 13C-uncoupled methyl protons resonance.
1H NMR simultaneous editing of 13C-coupled and 13C-uncoupled methyl protons resonance, using the selection of double quantum coherences by a gradient pulse, was analyzed in vitro and demonstrated in situ on the hindlimb of an exercised rat model postmortem. In vitro calibration showed agreement with theoretical analysis. High-resolution NMR of muscle extract confirmed the accuracy of the lactate 13C-enrichment calculated using the in situ NMR data and the calibration factor obtained in vitro.
Bloch
G
G
CEA, Service Hospitalier Fréderic Joliot, Département de Recherche en Imagerie, Pharmacologie et Physiologie, Orsay, France.
Jouvensal
L
L
Carlier
P G
PG
D000818:Animals; D002250:Carbon Radioisotopes; D006614:Hindlimb; D066298:In Vitro Techniques; D007773:Lactates / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D018482:Muscle, Skeletal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002250:Carbon Radioisotopes; D007773:Lactates