Effect of postischemic reperfusion on the pancreas.
In an examination of the effect of ischemia and reperfusion on the generation of active oxygen species during pancreatic cell damage, a short-term ischemia and reperfusion model was prepared by the occlusion and reperfusion of both the anterior mesenteric artery and the celiac artery in rats. Following 60 minutes of occlusion plus 7 hours of reperfusion of the anterior mesenteric artery and the celiac artery, the serum concentrations of amylase and lipase rose significantly to 7 and 6 times the respective control values. After 30 minutes of occlusion plus 7 hours of reperfusion, or after 7 hours of occlusion without reperfusion, amylase and lipase levels were not changed significantly. The continuous intravenous infusion of superoxide dismutase (3600 U/kg/hour) in rats receiving 60 minutes of occlusion plus 7 hours of reperfusion suppressed the rise in serum amylase and lipase values to 25 percent of the values in the non-injected group. These results suggest that the active oxygen species which are generated by the short-term ischemia and reperfusion method injure the endothelium and cause hyperamylasemia and hyperlipasemia. Inhibition of the rise in serum amylase and lipase concentrations by pretreatment with a scavenger of active oxygen, superoxide dismutase, suggests that the active oxygen species are involved in the pathogenesis of acute pancreatitis.
Tamura
K
K
First Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Manabe
T
T
Kyogoku
T
T
Andoh
K
K
Ohshio
G
G
Tobe
T
T
D000681:Amylases / Q000097:blood / Q000187:drug effects; D000818:Animals; D007511:Ischemia / Q000503:physiopathology*; D008049:Lipase / Q000097:blood / Q000187:drug effects; D008297:Male; D010179:Pancreas / Q000098:blood supply* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000503:physiopathology*; D013482:Superoxide Dismutase / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors
D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D008049:Lipase; D000681:Amylases