Development and application of a polymerase chain reaction assay for Mycoplasma synoviae.
Mycoplasma synoviae (MS) species-specific primers selected from the 16S rRNA sequence were evaluated by polymerase chain reaction (PCR). The MS primers were MS-1 (5'-GAAGCAAAATAGTGATATCA-3') and MS-2 (5'-GTCGTCTCCGAAGTTAACAA-3'). Analysis of cultures of avian mycoplasmas using the MS PCR indicated 100% specificity and sensitivity: 55 individual isolates of MS tested PCR-positive, and 44 isolates of eight other species of avian mycoplasmas tested PCR-negative. The MS PCR will detect 100 colony-forming units of MS. Analysis of 122 flock data sets indicated a sensitivity for the MS PCR test of 82% and a specificity of 100% as determined by comparison with culture, serology (serum plate test, hemagglutination-inhibition, enzyme-linked immunosorbent assay), epizootiology, and history.
Lauerman
L H
LH
Albama Department of Agriculture and Industries, C. S. Roberts Veterinary Diagnostic Laboratory, Auburn 36831-2209.
Hoerr
F J
FJ
Sharpton
A R
AR
Shah
S M
SM
van Santen
V L
VL
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005069:Evaluation Studies as Topic; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics*; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D011201:Poultry Diseases / Q000175:diagnosis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S