A truncated isoform of the thyrotropin-releasing hormone receptor is expressed in the rat central nervous system as well as in the pituitary gland.
Using the reverse transcription-polymerase chain reaction (RT-PCR), a cDNA encoding the entire rat thyrotropin-releasing hormone receptor (TRH-R) was isolated from normal rat pituitary gland mRNA. In addition, a novel truncated isoform of TRH-R which lacks 52 base pairs (bp) in the carboxyl (C-) terminal tail was isolated. This truncation, probably generated by alternate splicing, causes a frame-shift and results in a truncated TRH-R 25 amino acids shorter and with a different C-terminal amino acid sequence than the longer type receptor. This truncated TRH-R mRNA, along with the longer receptor form, was found to be expressed throughout the rat pituitary gland and brain.
Satoh
T
T
Division of Endocrinology, University of Maryland School of Medicine, Baltimore 21201.
Feng
P
P
Wilber
J F
JF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002490:Central Nervous System / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009928:Organ Specificity; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D017207:Rats, Sprague-Dawley; D018025:Receptors, Thyrotropin-Releasing Hormone / Q000096:biosynthesis* / Q000235:genetics
D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D018025:Receptors, Thyrotropin-Releasing Hormone; D012194:RNA-Directed DNA Polymerase