Neutralization of bovine papillomavirus by antibodies to L1 and L2 capsid proteins.
We have generated four mouse monoclonal antibodies (MAbs) to bovine papillomavirus virions that bound type-specific, adjacent, and conformationally dependent epitopes on the L1 major capsid protein. All four MAbs were neutralizing at ratios of 1 MAb molecule per 5 to 25 L1 molecules, but only three effectively blocked binding of the virus to the cell surface. Therefore, antibodies can prevent papillomavirus infection by at least two mechanisms: inhibition of cell surface receptor binding and a subsequent step in the infectious pathway. The neutralizing epitopes of the bovine papillomavirus L2 minor capsid protein were mapped to the N-terminal half of L2 by blocking the neutralizing activity of full-length L2 antiserum with bacterially expressed peptides of L2. In addition, rabbit antiserum raised against amino acids 45 to 173 of L2 had a neutralizing titer of 1,000, confirming that at least part of the N terminus of L2 is exposed on the virion surface.
Roden
R B
RB
Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland 20892.
Weissinger
E M
EM
Henderson
D W
DW
Booy
F
F
Kirnbauer
R
R
Mushinski
J F
JF
Lowy
D R
DR
Schiller
J T
JT
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D052688:Bovine papillomavirus 1 / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D000939:Epitopes; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000939:Epitopes; D010446:Peptide Fragments