Analysis of murine antibody responses to baculovirus-expressed human immunodeficiency virus type 1 envelope glycoproteins.
An analysis of the humoral immunogenicity of a candidate AIDS vaccine (VaxSyn) in a murine model system is presented. Sera taken from a panel of mice immunized with the immunogen were analysed for their ability to bind a panel of gp120-representing peptides and limited reactivity to known sites of immunological interest was observed. Monoclonal antibodies (MAbs) were characterized as binding to a more restricted variety of regions on gp120 including C1, V2, V4 and the C terminus. A tetrazolium-based cytocidicity assay was used and shown to be an effective and objective method for the screening of human immunodeficiency virus (HIV)-neutralizing activity in large numbers of samples. None of the MAbs characterized in this study neutralize HIV-1 reference strains. The significance of these findings in view of previous publications is discussed.
Bristow
R G
RG
Virology Division, National Institute for Medical Research, Mill Hill, London, U.K.
Douglas
A R
AR
Skehel
J J
JJ
Daniels
R S
RS
D016915:AIDS Vaccines / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D016367:Baculoviridae / Q000235:genetics; D000939:Epitopes; D006678:HIV / Q000276:immunology*; D015483:HIV Antibodies / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests / Q000379:methods; D010446:Peptide Fragments / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012795:Sialoglycoproteins / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
D016915:AIDS Vaccines; D000911:Antibodies, Monoclonal; D000939:Epitopes; D015483:HIV Antibodies; D010446:Peptide Fragments; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D014614:Vaccines, Synthetic; C053473:VaxSyn HIV-1 (gp160) vaccine