title: Polyamine transport in human promyelocytic leukemia cells and polymorphonuclear leukocytes.
abstract: We examined the kinetics of polyamine uptake by human myeloid cells at different stages of maturity. The Km values of putrescine, spermidine and spermine transport by HL-60 cells were 52, 7.9 and 8.1 microM, respectively. These values decreased to 5.1, 1.7 and 0.77 microM, respectively, in HL-60 cells induced to mature past the promyelocyte stage by DMSO. In human PMNs, the respective Km values were 501, 479 and 381 microM. Transport by HL-60 cells was enhanced when intracellular polyamine levels were reduced with difluoromethylornithine. Thus, HL-60 cell maturation is accompanied by an increase in the affinity of their polyamine transport system. This system is much more efficient than that found in end-stage PMNs, suggesting that it plays a more important role in supporting the metabolic requirements of HL-60 cells. Alternatively, the low affinity of the PMN polyamine transport system could represent an adaptation to the high polyamine concentrations found at infection sites.
authors.affiliation: Department of Periodontology, College of Dentistry, Ohio State University Health Sciences Center 43210.
authors.forename: J D
authors.initials: JD
authors.lastname: Walters
authors.forename: M S
authors.initials: MS
authors.lastname: Wojcik
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D001692:Biological Transport / Q000187:drug effects; D002460:Cell Line; D019833:Genistein; D006096:Gramicidin / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D015759:Ionomycin / Q000494:pharmacology; D007529:Isoflavones / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D010879:Piperazines / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011700:Putrescine / Q000378:metabolism* / Q000494:pharmacology; D013095:Spermidine / Q000378:metabolism* / Q000494:pharmacology; D013096:Spermine / Q000378:metabolism*; D013449:Sulfonamides; D013696:Temperature; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D007529:Isoflavones; D007546:Isoquinolines; D010879:Piperazines; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; D006096:Gramicidin; D013096:Spermine; D015759:Ionomycin; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; C053177:N-(2-(methylamino)ethyl)-5-isoquinolinesulfonamide; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D019833:Genistein; D013755:Tetradecanoylphorbol Acetate; D013095:Spermidine; D011700:Putrescine