Modified CAMP test for biogrouping Vibrio cholerae O1 strains and distinguishing them from strains of V. cholerae non-O1.
A modified CAMP test was used to identify 973 Vibrio cholerae isolates by phenotype. Eltor and non-O1 strains were CAMP positive; classical strains were CAMP negative. Sausage-shaped zones of hemolysis of eltor strains were easily distinguished from narrower bands of non-O1 isolates. For O1 isolates, there was 100% agreement between the CAMP test and inhibition by polymyxin B.
Lesmana
M
M
Department of Microbiology, U.S. Naval Medical Research Unit No. 2, Jakarta, Indonesia.
Subekti
D
D
Tjaniadi
P
P
Pazzaglia
G
G
D001426:Bacterial Proteins / Q000032:analysis*; D006460:Hemolysin Proteins / Q000032:analysis*; D019081:O Antigens; D010641:Phenotype; D011112:Polymyxin B / Q000494:pharmacology; D011135:Polysaccharides, Bacterial; D014734:Vibrio cholerae / Q000145:classification* / Q000187:drug effects
D001426:Bacterial Proteins; C039092:CAMP protein, Streptococcus; D006460:Hemolysin Proteins; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D011112:Polymyxin B