title: G-CSF induces tyrosine phosphorylation of the JAK2 protein in the human myeloid G-CSF responsive and proliferative cells, but not in mature neutrophils.
abstract: Granulocyte colony-stimulating factor (G-CSF) stimulates a rapid phosphorylation on tyrosines of several proteins of Mr. 130, 100, 90, 70, 44 kd in human myeloid leukemia cell line cells, Kasumi-1, which respond to G-CSF to proliferate in vitro. In HL60 cells, only a 100 kd protein was phosphorylated, and no detectable phosphorylated proteins were observed in neutrophils by the stimulation of G-CSF. Among these proteins, the 130 kd protein was immunoprecipitated by anti- JAK2 serum. While JAK2 is a non receptor tyrosine kinase and is reported to be involved in the signal transduction by various cytokines including growth hormone, erythropoietin, and granulocyte-macrophage colony-stimulating factor/interleukin-3, it is strongly suggested that a signaling pathway that relates to the cell proliferation triggered by G-CSF in immature hematopoietic cells also involves JAK2.
authors.affiliation: First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shimoda
authors.forename: H
authors.initials: H
authors.lastname: Iwasaki
authors.forename: S
authors.initials: S
authors.lastname: Okamura
authors.forename: Y
authors.initials: Y
authors.lastname: Ohno
authors.forename: A
authors.initials: A
authors.lastname: Kubota
authors.forename: F
authors.initials: F
authors.lastname: Arima
authors.forename: T
authors.initials: T
authors.lastname: Otsuka
authors.forename: Y
authors.initials: Y
authors.lastname: Niho
mesh_terms: D002455:Cell Division; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D007163:Immunosorbent Techniques; D053614:Janus Kinase 2; D007700:Kinetics; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism* / Q000473:pathology; D009504:Neutrophils / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D011518:Proto-Oncogene Proteins; D016179:Granulocyte Colony-Stimulating Factor; D019000:Phosphotyrosine; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; C507924:JAK2 protein, human; D053614:Janus Kinase 2