title: Characterization of two classes of ribonucleoprotein complexes possibly involved in RNA editing from Leishmania tarentolae mitochondria.
abstract: The molecular mechanism of RNA editing in trypanosomatid mitochondria is an unsolved problem. We show that two classes of ribonucleoprotein complexes exist in a mitochondrial extract from Leishmania tarentolae and appear to be involved in RNA editing. The 'G' class of RNP complexes consists of 170-300 A particles which contain guide RNAs and proteins, show little terminal uridylyl transferase (TUTase) activity and exhibit an in vitro RNA editing-like activity. The 'T' class consists of approximately six RNP complexes, the endogenous RNA of which can be self-labeled with [alpha-32P]UTP. The most abundant T complex, T-IV, is visualized by electron microscopy as 80-140 A particles. This complex exhibits TUTase activity in the native gel and contains guide RNAs. Both G and T complexes are possibly involved with RNA editing in vivo. These results are a starting point for the analysis of the biochemistry of RNA editing.
authors.affiliation: Department of Biology, UCLA School of Medicine, University of California 90024-1662.
authors.forename: M
authors.initials: M
authors.lastname: Peris
authors.forename: G C
authors.initials: GC
authors.lastname: Frech
authors.forename: A M
authors.initials: AM
authors.lastname: Simpson
authors.forename: F
authors.initials: F
authors.lastname: Bringaud
authors.forename: E
authors.initials: E
authors.lastname: Byrne
authors.forename: A
authors.initials: A
authors.lastname: Bakker
authors.forename: L
authors.initials: L
authors.lastname: Simpson
mesh_terms: D000818:Animals; D001483:Base Sequence; D007891:Leishmania / Q000235:genetics*; D008928:Mitochondria / Q000502:physiology*; D008969:Molecular Sequence Data; D012313:RNA; D017393:RNA Editing; D012316:RNA Nucleotidyltransferases / Q000032:analysis; D017394:RNA, Guide, Kinetoplastida / Q000032:analysis; D000077278:RNA, Mitochondrial; D016053:RNA, Protozoan / Q000235:genetics*; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000648:ultrastructure; D014544:Uridine Triphosphate / Q000378:metabolism
chemical_list: D000077278:RNA, Mitochondrial; D016053:RNA, Protozoan; D012261:Ribonucleoproteins; D012313:RNA; D012316:RNA Nucleotidyltransferases; C031687:UTP-RNA uridylyltransferase; D014544:Uridine Triphosphate; D017394:RNA, Guide, Kinetoplastida