Oligoclonal beta-galactoside-binding immunoglobulins antigenically related to 14 kDa lectin in human serum and cerebrospinal fluid: purification and characterization.
1. An antiserum raised against a 14 kDa beta-galactoside specific lectin from human brain (HBL14) was used to probe blots from samples of serum and cerebrospinal fluid. The only HBL14-immunoreactive material detected was heavy and light chains of a beta-galactoside-binding IgG fraction (lectin-like IgG). 2. Lectin-like IgG, as well as IgG Fab fragments, compete with HBL14 for binding either to anti-HBL14 antibody or to a lactosyl polyacrylamide-based copolymer. 3. Purification of lectin-like IgG was obtained by affinity chromatography on immobilized rabbit anti-lectin immunoglobulins. The carbohydrate-binding specificity of the purified molecules was restricted to beta-Gal-containing structures and close to the HBL14 one.
Joubert-Caron
R
R
Laboratoire de Biochimie et Technologie des Protéines, Université Paris-Nord, Bobigny, France.
Caron
M
M
Bochet
P
P
Chadli
A
A
Delaporte
P
P
Schuller
E
E
Bladier
D
D
D000595:Amino Acid Sequence; D001667:Binding, Competitive; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005697:Galactosides / Q000378:metabolism*; D037161:Galectins; D006388:Hemagglutinins / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology* / Q000302:isolation & purification; D037102:Lectins / Q000097:blood / Q000134:cerebrospinal fluid / Q000276:immunology* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017386:Sequence Homology, Amino Acid
D000939:Epitopes; D005697:Galactosides; D037161:Galectins; D006388:Hemagglutinins; D007074:Immunoglobulin G; D037102:Lectins; C007816:beta-galactoside