Motionally restricted tryptophan environments at the peptide-lipid interface of gramicidin channels.
The tryptophans in the gramicidin channel play a crucial role in the organization and function of the channel. The localization and dynamics of these tryptophans have been studied using fluorescence spectroscopy, especially utilizing environment-induced effects on the rates of solvent relaxation around these residues in membranes. When incorporated into model membranes of dioleoyl-sn-glycero-3-phosphocholine (DOPC), the tryptophans in the gramicidin channel exhibit a red edge excitation shift (REES) of 6 nm. In addition, fluorescence polarization shows both excitation and emission wavelength dependence. Fluorescence lifetime analysis shows a biexponential decay, corresponding to a short- and a long-lifetime component. The mean lifetime was found to be dependent on both excitation and emission wavelengths. Analysis of time-resolved emission spectra (TRES) shows a heterogeneous environment for the tryptophans consistent with the lifetime information. Taken together, these observations point out the motional restriction experienced by the tryptophans in the gramicidin channel. This is consistent with other studies in which such restrictions are thought to be imposed due to hydrogen bonding between the indole rings of the tryptophans and the neighboring lipid carbonyls. The significance of such organization in terms of functioning of the channel is brought out by the fact that substitution, photodamage, or chemical modification of these tryptophans is known to give rise to channels with conformation and reduced conductivity.
Mukherjee
S
S
Centre for Cellular and Molecular Biology, Hyderabad, India.
Chattopadhyay
A
A
D006096:Gramicidin / Q000737:chemistry*; D007473:Ion Channels; D008051:Lipid Bilayers; D008433:Mathematics; D008961:Models, Structural; D008962:Models, Theoretical; D010713:Phosphatidylcholines; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence / Q000379:methods; D014364:Tryptophan
D007473:Ion Channels; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D006096:Gramicidin; D014364:Tryptophan; C017251:1,2-oleoylphosphatidylcholine