title: Artificial protein vaccines with predetermined tertiary structure: application to anti-HIV-1 vaccine design.
abstract: A successful approach to the development of a safe and effective synthetic vaccine requires that different B and T cell epitopes of the infectious agent be included in the vaccine construction. In this paper we suggest a new approach to vaccine design in the form of an artificial protein with a predetermined tertiary structure (PTS vaccines). Based on B and T cell epitope properties, we substantiate the possible use for vaccine construction of one well-known protein spatial motif--the four-alpha-helix bundle. Antigenic determinants of cellular immunity (amphipathic alpha-helices) and humoral immunity (flexible hydrophilic loop regions) are used as blocks for vaccine design. General principles of PTS vaccine construction have been applied to anti-HIV-1 vaccine design.
authors.affiliation: Research Institute of Molecular Biology, NPO VECTOR, Koltsovo, Novosibirsk Region, Russia.
authors.forename: A M
authors.initials: AM
authors.lastname: Eroshkin
authors.forename: P A
authors.initials: PA
authors.lastname: Zhilkin
authors.forename: V V
authors.initials: VV
authors.lastname: Shamin
authors.forename: S
authors.initials: S
authors.lastname: Korolev
authors.forename: B B
authors.initials: BB
authors.lastname: Fedorov
mesh_terms: D016915:AIDS Vaccines / Q000737:chemistry*; D015195:Drug Design; D000939:Epitopes / Q000737:chemistry*; D015686:Gene Products, env / Q000737:chemistry; D015683:Gene Products, gag / Q000737:chemistry; D005813:Genes, Synthetic; D015488:HIV Antigens / Q000737:chemistry*; D015497:HIV-1 / Q000276:immunology*; D008958:Models, Molecular; D008962:Models, Theoretical; D015202:Protein Engineering; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic / Q000737:chemistry*
chemical_list: D016915:AIDS Vaccines; D000939:Epitopes; D015686:Gene Products, env; D015683:Gene Products, gag; D015488:HIV Antigens; D011506:Proteins; D011994:Recombinant Proteins; C085784:TBI protein, recombinant; D014614:Vaccines, Synthetic