An improved experimental model for the study of in vitro release of nitric oxide by murine peritoneal macrophages.
In the immune system macrophages are the cells responsible for nitric oxide (NO) production. The synthesis of NO by activated macrophages correlates with their cytotoxic effect on neoplastic cells as well as killing of intracellular parasites. In the present paper we test several parameters that may influence (in vitro) NO production by murine peritoneal macrophages previously stimulated in vivo by intraperitoneal injection of thioglycollate. In our system the maximum NO/NO2- release was obtained in the culture containing 10(6) M phi/ml after 24 h incubation. For macrophage activation we used lipopolysaccharide (LPS) and several recombinant cytokines (IFN-gamma, TNF-alpha, IL-2, IL-3, IL-6). We also tested the influence of latex phagocytosis on NO production by simultaneously activated macrophages.
Marcinkiewicz
J
J
Department of Immunology, Jagiellonian University Collegium Medicum, Cracow, Poland.
Pater
M
M
Grabowska
A
A
D000818:Animals; D001120:Arginine / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009569:Nitric Oxide / Q000096:biosynthesis / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D010587:Phagocytosis; D013268:Stimulation, Chemical
D016207:Cytokines; D008070:Lipopolysaccharides; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase