Vascular activation in the histopathogenesis of Hodgkin's disease: potential role of endothelial tissue factor in intravascular thrombosis and necrosis.
Endothelial cell activation and alterations of intravascular coagulation were investigated in 27 cases of Hodgkin's disease (HD), in five cases of anaplastic large cell lymphoma (ALCL), and in ten reactive lymph nodes. Lymph node sections were immunostained for E-selectin, a molecule present on cytokine-activated endothelial cells; for tissue factor (TF), a cellular initiator of the coagulation cascade; for glycoprotein (gp) II/III, a platelet-specific antigen; and for fibrin. In HD, vascular activation was particularly prominent in the nodular sclerosis subtype, as indicated by a larger number of E-selectin-positive blood vessels (72 +/- 49) compared with mixed cellularity (22 +/- 37). High expression of E-selectin was associated with alterations of intravascular coagulation, as indicated by immunostaining of some vascular endothelial cells for TF, by a higher incidence of intravascular thrombi, and by the extensive presence of areas of fibrin exudation and necrosis. In ALCL, the levels of endothelial cell activation and intravascular coagulation were comparable to those of HD nodular sclerosis. In reactive nodes, some E-selectin-positive blood vessels were observed only in 3/10 cases; immunostaining for TF was not detected on endothelial cells; and alterations of intravascular coagulation were rarely observed.
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Dipartimento di Medicina Sperimentale, Università di Roma La Sapienza, Italy.
Pittiglio
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Dejana
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Baroni
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D000293:Adolescent; D000328:Adult; D000368:Aged; D015815:Cell Adhesion Molecules / Q000032:analysis; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000503:physiopathology*; D005260:Female; D005337:Fibrin / Q000032:analysis; D006689:Hodgkin Disease / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008198:Lymph Nodes / Q000098:blood supply / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009336:Necrosis; D009363:Neoplasm Proteins / Q000032:analysis; D012039:Regional Blood Flow; D013925:Thromboplastin / Q000502:physiology*; D013927:Thrombosis / Q000209:etiology*
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D009363:Neoplasm Proteins; D005337:Fibrin; D013925:Thromboplastin