[An immunosorbent for interferon purification].
Immunoaffinity chromatography was shown to be the method achieving the most complete elimination of antigenic admixtures from leukocyte interferon preparations without the loss of the preparation activity. An affinity sorbent has been developed on the basis of covalently linked polyvinyl alcohol (PVA). The immobilization of the antigen-specific rabbit globulin in the preparation of the sorbent is achieved by reaction of protein amino groups with the activated matrix. The proposed sorbent achieved the elimination of the antigenic admixtures from interferon preparations as effectively as those prepared on the basis of sepharose 4B, the productivity of the purification process being at least 5 times higher. The proposed sorbent is stable at the limit values of rH, is not destroyed by detergents, is sterilized in the process of preparation. Owing to the strong linkage of the immobilized immunoglobulin with the PVA-carrier, immunoaffinity chromatography on this sorbent does not involve contamination of the preparations with rabbit globulin allergenic for man. The combination of a large pore structure, wetting ability, stiffness, mechanical and chemical stability allows the proposed sorbent to be recommended for use in modern large-scale biotechnological production.
Garipova
M I
MI
Monakov
Iu B
IuB
Baschenko
I A
IA
Frolova
I S
IS
Kleeva
O B
OB
Kuznetsov
V P
VP
D000818:Animals; D000941:Antigens / Q000302:isolation & purification; D002478:Cells, Cultured; D002846:Chromatography, Affinity / Q000379:methods; D004340:Drug Contamination; D006801:Humans; D007163:Immunosorbent Techniques; D007164:Immunosorbents; D007369:Interferon Inducers / Q000494:pharmacology; D007372:Interferons / Q000302:isolation & purification*; D007962:Leukocytes / Q000187:drug effects; D010047:Ovalbumin / Q000302:isolation & purification; D011142:Polyvinyl Alcohol; D011817:Rabbits
D000941:Antigens; D007164:Immunosorbents; D007369:Interferon Inducers; D011142:Polyvinyl Alcohol; D010047:Ovalbumin; D007372:Interferons