Diversity in molecular mass of the common EDTA-soluble antigens of Clostridium chauvoei and Clostridium septicum.
Common EDTA-soluble antigens of Clostridium chauvoei and C. septicum were examined by indirect-immunofluorescence (IFA) and immunoblot analysis. The monoclonal antibodies (mAbs) specific for the 35 kDa antigen of C. chauvoei strain ATCC 10092 were used. These mAbs reacted with all 11 strains, 6 of C. chauvoei and 5 of C. septicum, in IFA. In immunoblot analysis with the mAbs, the bands at molecular mass of 35 kDa were found in all C. chauvoei strains, while the bands at 36 kDa were found in 4 of 5 strains of C. septicum. These results indicate that the 35 kDa antigen of C. chauvoei and the 36 kDa antigen of C. septicum possess a similar epitope recognized by the mAb.
Hamaoka
T
T
Touhoku Branch Laboratory, National Institute of Animal Health, Aomori, Japan.
Terakado
N
N
Nakamura
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D000954:Antigens, Surface / Q000737:chemistry / Q000302:isolation & purification; D003013:Clostridium / Q000145:classification / Q000276:immunology*; D003429:Cross Reactions; D004492:Edetic Acid; D000939:Epitopes / Q000737:chemistry / Q000302:isolation & purification; D005455:Fluorescent Antibody Technique; D015151:Immunoblotting; D051379:Mice; D008970:Molecular Weight; D012995:Solubility; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D000939:Epitopes; D004492:Edetic Acid