Von Willebrand factor in the rat kidney: its localization and the effects of its in vivo interaction with specific antibodies.
Antiendothelial cell antibodies and increased von Willebrand factor (vWF) levels are observed in many renal diseases. We studied renal morphology after administration of rabbit anti-human factor VIII-von Willebrand factor (FVIII-vWFc) gamma-globulin (IgG) in rats. Isolated perfusion of normal rat kidney with the antibodies disclosed rabbit IgG along glomerular endothelium and in mesangial areas. Systemic administration resulted in an identical antibody distribution. C3 deposits and few electron dense deposits were transiently noted. After 1 week the mesangial deposition of rabbit IgG had increased significantly, most likely reflecting increased release of vWF by the endothelium. Thereafter, the deposits gradually relocated into mesangial stalk and into the glomerular vascular pole, and they had vanished after 6 weeks. Except for a mild influx of leukocytes, no renal injury occurred. In conclusion, although FVIII-vWFc antibodies cause mesangial immune deposits, antibodies against other endothelial antigens are apparently needed to inflict renal damage.
Ito
S
S
Department of Pathology, State University of New York at Buffalo.
Hirabayashi
K
K
Nielsen
N
N
Ueda
Y
Y
Brentjens
J R
JR
Gans
R O
RO
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology*; D000918:Antibody Specificity; D003429:Cross Reactions; D004727:Endothelium / Q000737:chemistry / Q000187:drug effects / Q000276:immunology; D005169:Factor VIII / Q000032:analysis / Q000276:immunology / Q000494:pharmacology; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007668:Kidney / Q000737:chemistry / Q000187:drug effects / Q000276:immunology*; D007678:Kidney Glomerulus / Q000737:chemistry / Q000187:drug effects / Q000276:immunology; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D005719:gamma-Globulins / Q000494:pharmacology; D014841:von Willebrand Factor / Q000032:analysis* / Q000276:immunology / Q000494:pharmacology
D000906:Antibodies; D007074:Immunoglobulin G; D005719:gamma-Globulins; D014841:von Willebrand Factor; D005169:Factor VIII