[Effect of thyrotropin releasing hormone and histidyl-proline diketopiperazine on enzyme section of isolated pancreatic acinar cells of the rat].
The effect of thyrotropin-releasing hormone (TRH) and histidyl-proline-diketopiperazine [cyclo (His-Pro), CHP] on amylase secretion from isolated rat exocrine pancreatic acinar cells was investigated. TRH showed a dose-dependent inhibition of carbachol-stimulated amylase secretion with a maximum of 24% at a concentration of 10(-11) M (p < 0.05). Basal amylase release was not affected in concentrations from 10(13) M to 10(-8) M. CHP did not influence basal or carbachol-stimulated pancreatic secretion in vitro. These results suggest that TRH in contrast to CHP may play a role in the paracrine regulation of exocrine pancreatic secretion.
Kemmer
T P
TP
Abteilung Innere Medizin I, Universität Ulm.
Duntas
L
L
Munz
W
W
Malfertheiner
P
P
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002478:Cells, Cultured; D008297:Male; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology*; D010179:Pancreas / Q000166:cytology*; D010456:Peptides, Cyclic / Q000494:pharmacology*; D010879:Piperazines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012636:Secretory Rate / Q000187:drug effects; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology*
D014179:Neurotransmitter Uptake Inhibitors; D010456:Peptides, Cyclic; D010879:Piperazines; D013973:Thyrotropin-Releasing Hormone; D002217:Carbachol; D000681:Amylases; C015924:histidyl-proline diketopiperazine