Metalloprotease and serine protease are involved in cleavage of CD43, CD44, and CD16 from stimulated human granulocytes. Induction of cleavage of L-selectin via CD16.
CD43, CD44, CD16, and L-selectin have been previously shown to be enzymatically cleaved from stimulated leukocytes. However, little is known about the enzymes involved in these processes. Here, metalloprotease(s) inhibitable by 1,10-phenanthroline together with serine protease(s) inhibitable by N alpha-p-tosyl-L-lysine chloromethyl ketone and 3,4-dichloroisocoumarin are shown to be involved in the cleavage of CD43, CD44, and CD16 but not in the cleavage of L-selectin on granulocytes. In addition, mAbs that recognize these individual receptors and induce their specific cleavage did not initiate cleavage of the others. In one case only, L-selectin, cleavage was also triggered by mAbs interacting with CD16 (the low affinity Fc gamma R). Thus, this mechanism represents a novel pathway of L-selectin cleavage induction.
Bazil
V
V
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.
Strominger
J L
JL
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D002352:Carrier Proteins / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D015536:Down-Regulation; D004492:Edetic Acid / Q000494:pharmacology; D005434:Flow Cytometry; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D018960:Hyaluronan Receptors; D019041:L-Selectin; D051917:Leukosialin; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors*; D010618:Phenanthrolines / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000378:metabolism*; D017452:Receptors, IgG / Q000378:metabolism*; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology*; D012795:Sialoglycoproteins / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D051917:Leukosialin; D010618:Phenanthrolines; D011956:Receptors, Cell Surface; D017452:Receptors, IgG; D016201:Receptors, Lymphocyte Homing; C465014:SPN protein, human; D015842:Serine Proteinase Inhibitors; D012795:Sialoglycoproteins; D019041:L-Selectin; D014107:Tosyllysine Chloromethyl Ketone; D004492:Edetic Acid; D008666:Metalloendopeptidases; D013755:Tetradecanoylphorbol Acetate; C025205:1,10-phenanthroline