Isolation and characterization of the major equilibrium product of FK-520.
The existence of an isomeric form in equilibrium with the major component of FK-520 in polar solutions has been demonstrated. This minor component has been isolated in high yield and purity by a novel crystallization strategy and preparative HPLC. The equilibrium product was characterized by NMR and MS.
Gailliot
F P
FP
Merck Research Laboratories, Rahway, NJ 07065-0900.
Natishan
T K
TK
Ballard
J M
JM
Reamer
R A
RA
Kuczynski
D
D
Mcmanemin
G J
GJ
Egan
R S
RS
Buckland
B C
BC
D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D003460:Crystallization; D007166:Immunosuppressive Agents / Q000737:chemistry* / Q000302:isolation & purification*; D007536:Isomerism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry; D013056:Spectrophotometry, Ultraviolet; D016559:Tacrolimus / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D007166:Immunosuppressive Agents; C058028:immunomycin; D016559:Tacrolimus