[Detection of the generation of nitric oxide from L-arginine in the murine brain in vivo using EPR].
Nitric oxide (NO) was shown by EPR method to be generated via L-arginine-dependent way in brain of mice in vivo. The complexes of diethyldithiocarbamate (DETC) or pirrolidinedithiocarbamate (PDTC) with endogenous or exogenous Fe2+ were used as a traps of NO, which are capable to bind NO resulted in the formation of mononitrosyl iron complexes with DETC or PDTC (MNIC-DETC or PDTC) recovered by EPR method. These complexes were detected in mouse brain in concentration of 2.5 nmole/g of wet tissue for 30 min only when exogenous Fe2+ was injected in to mice. The level of MNIC-DETC (PDTC) was 5 fold increased in brain of mice pretreated for 4 hrs with lipopolysaccharide from Escherichia coli, which induced the inflammation processes. The inhibitor of NO-synthase, NG-nitro-L-arginine attenuated the formation of MNIC-DETC (PDTC) in mouse brain in vivo. Exogenous Fe2+ is suggested to induced the synthesis of NO-synthase in mouse brain.
Mikoian
V D
VD
Kubrina
L N
LN
Vanin
A F
AF
D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis; D000818:Animals; D001120:Arginine / Q000378:metabolism*; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D004121:Dimethyl Sulfoxide; D004050:Ditiocarb / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D007501:Iron / Q000737:chemistry; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase; D011759:Pyrrolidines / Q000737:chemistry; D013859:Thiocarbamates / Q000737:chemistry
D011759:Pyrrolidines; D013859:Thiocarbamates; C020972:pyrrolidine dithiocarbamic acid; D009569:Nitric Oxide; D001120:Arginine; D004050:Ditiocarb; D007501:Iron; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D004121:Dimethyl Sulfoxide