Characterization of the acute phase serum protein response in pigs.
Acute inflammation was induced in pigs using a single subcutaneous turpentine injection. The acute phase serum protein response was analyzed using crossed immunoelectrophoresis and immunodiffusion. The concentration of C reactive protein and haptoglobin increases 5-7 times 48 h after the injection, whereas the concentration of an alpha 2-globulin, named pig major acute phase protein (pig-MAP), increases at least 15-fold. A molecular mass of 115 kDa for pig-MAP was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This protein did not crossreact with antisera to human hemopexin, ceruloplasmin, H-kininogen and complement factor C3. Albumin and alpha-lipoprotein were negative acute phase proteins because their concentration significantly decreased during inflammation. Finally, the concentration of alpha 1-acid glycoprotein, fetuin, alpha 1-protease inhibitor, transferrin and alpha 2-macroglobulins, as well as total proteins, did not change significantly during inflammation.
Lampreave
F
F
Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Ciencias, Universidad de Zaragoza, Spain.
González-Ramón
N
N
Martínez-Ayensa
S
S
Hernández
M A
MA
Lorenzo
H K
HK
García-Gil
A
A
Piñeiro
A
A
D000209:Acute-Phase Proteins / Q000378:metabolism*; D000210:Acute-Phase Reaction / Q000097:blood* / Q000139:chemically induced; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D013552:Swine; D014425:Turpentine
D000209:Acute-Phase Proteins; D014425:Turpentine