[The mite allergen and allergoid stimulation of histamine secretion by mast cells].
Rat peritoneal mast cells were incubated with serum from highly mite-sensitive patients. It was demonstrated that exposure of passive sensitized mast cells to allergen from mites Dermatophagoides farinae induced the release of histamine. Exposure of mast cells to 10 micrograms/ml and 50 micrograms/ml mite allergen resulted in an increase of histamine secretion to 48% of the basal level. The allergoid (formaldehyde-modified mite allergen) had poor histamine-releasing activity compared to allergen. The allergoid (50 micrograms/ml) induced a 2.5-fold decrease in histamine release. The allergen at the same concentrations and the same release as allergen in dose 0.1 microgram/ml.
Khlgatian
S V
SV
Perova
N A
NA
Berzhets
V M
VM
D000485:Allergens / Q000494:pharmacology*; D000080384:Allergoids; D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D006636:Histamine Release / Q000187:drug effects*; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D008925:Mites / Q000276:immunology*; D010529:Peritoneal Cavity / Q000166:cytology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D013268:Stimulation, Chemical
D000485:Allergens; D000080384:Allergoids; D010936:Plant Extracts