title: Rapid purification of recombinant Taq DNA polymerase by freezing and high temperature thawing of bacterial expression cultures.
authors.affiliation: Medical Biochemistry Department, University of the Witwatersrand Medical School, Parktown, South Africa.
authors.forename: E
authors.initials: E
authors.lastname: Grimm
authors.forename: P
authors.initials: P
authors.lastname: Arbuthnot
mesh_terms: D004259:DNA-Directed DNA Polymerase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005615:Freezing; D006358:Hot Temperature; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D019914:Taq Polymerase; D013824:Thermus / Q000201:enzymology* / Q000235:genetics; D013997:Time Factors
chemical_list: D011994:Recombinant Proteins; D019914:Taq Polymerase; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase