Distinguishing between duplex and hairpin forms of RNA by 15N-1H heteronuclear NMR.
A general method is described for distinguishing RNA hairpins from RNA duplexes by application of two-dimensional filtered nuclear Overhauser enhancement spectra on a 1:1 mixture of unlabeled and 99% 15N-labeled molecules. The method is applied to the RNA dodecamer rGGCGCUUGCGUC which can form an intramolecular hairpin under low salt conditions and a duplex in high salt. This procedure allows unambiguous identification of RNA hairpins or duplexes under the same conditions that are used in the NMR solution structure determination.
Aboul-ela
F
F
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215.
Nikonowicz
E P
EP
Pardi
A
A
D001482:Base Composition; D001483:Base Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*
D012313:RNA