title: Activation of the alternative pathway of complement by apoptotic Jurkat cells.
abstract: Jurkat T cells die of apoptosis upon exposure to anti-Fas mAb. Here we show that although the alternative complement pathway generally does not attack homologous cells, anti-Fas-induced apoptotic Jurkat T cells were attacked antibody-independently by the alternative pathway of human complement and opsonized with iC3b, which is a ligand of the complement receptor type 3 (CR3) of phagocytes. These results suggest that apoptotic cells become the targets of the homologous alternative complement pathway, which facilitates the clearance of apoptotic cells by phagocytes.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Matsui
authors.forename: S
authors.initials: S
authors.lastname: Tsuji
authors.forename: H
authors.initials: H
authors.lastname: Nishimura
authors.forename: S
authors.initials: S
authors.lastname: Nagasawa
mesh_terms: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000276:immunology; D017209:Apoptosis; D015153:Blotting, Western; D002460:Cell Line; D003179:Complement C3b / Q000378:metabolism; D003170:Complement Pathway, Alternative; D006801:Humans; D011951:Receptors, Complement / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D019014:fas Receptor
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D011951:Receptors, Complement; D019014:fas Receptor; D003179:Complement C3b