title: Role of nitric oxide in the peristalsis in the isolated guinea-pig ileum.
abstract: The role of nitric oxide in peristalsis was studied by using NG-nitro-L-arginine, an inhibitor of the biosynthesis of nitric oxide, in the isolated guinea-pig ileum. Constant peristalsis was cyclically induced by distending the ileal wall with intraluminal solution infused at a constant rate. NG-Nitro-L-arginine (10(-6) to 10(-4) M) dose dependently increased the frequency of peristalsis. This effect of NG-nitro-L-arginine was almost hindered by pretreating the ileum with 10(-3) M L-arginine, but not D-arginine. Nitroprusside (5 X 10(-7) M) reversed the frequency increase. In the presence of NG-nitro-L-arginine, peristaltic propulsion occurred at a smaller distension of the ileal wall and the ileum constricted to a smaller diameter at the completion of propulsion. The rate of shortening of longitudinal muscle during distension was raised by NG-nitro-L-arginine, although the peak magnitudes were not changed. Consistent with these effects of NG-nitro-L-arginine on peristalsis, NG-nitro-L-arginine at 10(-5) M increased the contractions of circular muscle in response to electrical field stimulation, but not those of longitudinal muscle. These results suggest that endogenous nitric oxide modulates peristalsis by limiting the contractile activity of the circular muscle of the guinea-pig ileum.
authors.affiliation: Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Mizuno
authors.forename: Y
authors.initials: Y
authors.lastname: Gomi
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D004558:Electric Stimulation; D006168:Guinea Pigs; D007082:Ileum / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D008954:Models, Biological; D009119:Muscle Contraction / Q000187:drug effects; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase; D019335:Nitroarginine; D010528:Peristalsis / Q000502:physiology*
chemical_list: D016210:Methacholine Chloride; D019335:Nitroarginine; D009569:Nitric Oxide; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases