title: Bartonella (Rochalimaea) quintana endocarditis in three homeless men.
abstract: BACKGROUND
  Bartonella (Rochalimaea) quintana is the agent of trench fever and is transmitted by the body louse. We searched for this organism in three alcoholic homeless men with endocarditis.
  
  
  METHODS
  Blood samples were cultured on a human endothelial cell line and on blood agar. Bacteria were identified by sequencing the amplified 16S ribosomal RNA gene. The presence of bartonella in tissue was assessed by Gram's staining, immunostaining, and polymerase-chain-reaction amplification. Serologic studies for antibodies to bartonella species were performed by indirect immunofluorescence and Western immunoblotting.
  
  
  RESULTS
  B. quintana was isolated from one patient in the blood-agar culture and from the other two patients in the endothelial-cell culture. The organism was also identified by both immunostaining and molecular techniques in the valvular vegetations from the three patients and in a cervical lymph node from one patient. The 16S ribosomal RNA gene sequences of the three isolates were almost identical to that of the prototype strain of B. quintana. High titers of antibodies to B. quintana were detected in all three patients, but so were cross-reacting antibodies to chlamydia species. In all three patients studies were repeatedly negative for antibodies to the human immunodeficiency virus.
  
  
  CONCLUSIONS
  B. quintana is a cause of endocarditis in homeless patients and may be serologically misdiagnosed as a chlamydial infection.
authors.affiliation: Unité des Rickettsies, Faculté de Médecine, Marseilles, France.
authors.forename: M
authors.initials: M
authors.lastname: Drancourt
authors.forename: J L
authors.initials: JL
authors.lastname: Mainardi
authors.forename: P
authors.initials: P
authors.lastname: Brouqui
authors.forename: F
authors.initials: F
authors.lastname: Vandenesch
authors.forename: A
authors.initials: A
authors.lastname: Carta
authors.forename: F
authors.initials: F
authors.lastname: Lehnert
authors.forename: J
authors.initials: J
authors.lastname: Etienne
authors.forename: F
authors.initials: F
authors.lastname: Goldstein
authors.forename: J
authors.initials: J
authors.lastname: Acar
authors.forename: D
authors.initials: D
authors.lastname: Raoult
mesh_terms: D000328:Adult; D000437:Alcoholism / Q000150:complications; D001473:Bartonella; D018413:Bartonella quintana / Q000302:isolation & purification; D004697:Endocarditis, Bacterial / Q000382:microbiology*; D006703:Ill-Housed Persons; D006801:Humans; D008297:Male; D008875:Middle Aged; D014205:Trench Fever / Q000175:diagnosis / Q000382:microbiology