The use of quartz patch pipettes for low noise single channel recording.
Quartz has a dissipation factor of approximately 10(-4), which is an order of magnitude less than that of the best glasses previously used to fabricate patch pipettes; it's dielectric constant of 3.8 is also lower than that of other glasses. On the basis of these electrical characteristics it is expected that patch pipettes pulled from quartz tubing will produce significantly less noise than pipettes made from other glasses. Our work confirms these expectations and we describe theoretical and practical aspects of the use of quartz pipettes for single channel patch voltage clamp measurements. Methods for pulling quartz pipettes with a laser-based puller and coating them with low-loss elastomers are discussed, as are precautions that are necessary to achieve low noise recordings. We have shown that quartz pipettes can be pulled from tubing with outer diameter to inner diameter ratios as large as 3 and a method of applying heavy elastomer coatings all the way to the tip of pipettes is presented. Noise sources arising from the pipette and its holder are described theoretically, and it is shown that measured noise is in good agreement with such predictions. With low noise capacitive feedback electronics, small geometry holders, and thick-walled quartz pipettes coated with low-loss elastomers we have been routinely able to achieve noise of 100 fA rms or less in a 5-kHz bandwidth with real cell patches and a pipette immersion depth of approximately 2 mm. On occasion we have achieved noise as low as 60 fA rms in this bandwidth.
Levis
R A
RA
Department of Physiology, Rush Medical College, Chicago, Illinois 60612.
Rae
J L
JL
D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D017097:Electric Impedance; D004563:Electrochemistry / Q000295:instrumentation; D005069:Evaluation Studies as Topic; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism*; D008839:Microelectrodes; D008962:Models, Theoretical; D011791:Quartz; D012408:Rubber
D007473:Ion Channels; D011791:Quartz; D012408:Rubber