title: Expression of glycolipid blood group antigens in single human kidneys: change in antigen expression of rejected ABO incompatible kidney grafts.
abstract: Total neutral glycolipid fractions were separated into molecular species on thin-layer chromatography plates and detected by immunostaining with monoclonal anti-blood group antibodies. Blood group A antigens based on type 1, 2, 3 and 4 carbohydrate core saccharides were present in kidneys of A1 and A1B individuals. Blood group A2 individuals expressed only small amounts of A antigen compared to A1 individuals especially of the type 3 and 4 compounds. Kidneys from non-secretor individuals contained less A antigen compared to secretor individuals, and in both groups a variation in the antigen expression between single individuals was noted. Blood group A type 2 and 3 (which is an extension of A type 2) antigens were present both as basic 6 and 9 sugar structures as well as extended saccharide chains migrating in the 8 to 11 sugar interval. In contrast, the type 1 chain based A and Lewis antigens were only present as their basic 5 to 7 sugar chains, and no elongated structures were found. Four cases of A2 kidneys initially transplanted into O recipients and removed after 5, 12, 21 days and 4 years, respectively, were also analyzed. Two of these kidneys, originating from the same donor, showed a difference in A antigen expression. The kidney functioning for four years (lost due to chronic rejection) completely lacked X antigen with five sugar residues (present in all other individuals) and contained a large amount of A antigens.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Regional Blood Center, Sahlgren's University Hospital, Göteborg, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Ulfvin
authors.forename: A E
authors.initials: AE
authors.lastname: Bäcker
authors.forename: H
authors.initials: H
authors.lastname: Clausen
authors.forename: S
authors.initials: S
authors.lastname: Hakomori
authors.forename: L
authors.initials: L
authors.lastname: Rydberg
authors.forename: B E
authors.initials: BE
authors.lastname: Samuelsson
authors.forename: M E
authors.initials: ME
authors.lastname: Breimer
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D001345:Autoradiography; D001789:Blood Group Antigens / Q000276:immunology; D001787:Blood Group Incompatibility; D002855:Chromatography, Thin Layer; D006017:Glycolipids / Q000378:metabolism*; D006084:Graft Rejection / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D007519:Isoantigens / Q000276:immunology*; D007668:Kidney / Q000378:metabolism*; D007983:Lewis Blood Group Antigens / Q000276:immunology; D013194:Staining and Labeling
chemical_list: D000017:ABO Blood-Group System; D001789:Blood Group Antigens; D006017:Glycolipids; D007519:Isoantigens; D007983:Lewis Blood Group Antigens