[Effects of 764-3 on the organization and function of microtubules and microfilaments in bleomycin A6 activated rat alveolar macrophages].
Using bleomycin A6 (BLM A6) to stimulate normal and 764-3 pretreated alveolar macrophages (AM) in vitro, the distributions of microtubules (MTs) and microfilaments (MFs) were examined with an immunofluorescence microscope. Fluorescent strength was determined with a spectrophotometer and fluorospectrophotometer, and the adhering and spreading rates were determined. The results showed that BLM A6-stimulated AM spread fully and MTs increased markedly, forming a thick network in the cytoplasm. Much punctate and linear fluorescence was visible beneath the plasma membrane, these fluorescing structures were MFs. In the experimental cells MT and MF fluorescence was stronger than that in the control group. However, most of the above changes were reduced in the 764-3 pretreatment group. These results suggest that BLM A6 may promote the assembly and regulate reorganization of MTs and MFs, while 764-3 can stabilize these structures.
Zhang
H
H
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing.
D008841:Actin Cytoskeleton / Q000187:drug effects*; D000818:Animals; D001761:Bleomycin / Q000494:pharmacology* / Q000031:analogs & derivatives; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016676:Macrophages, Alveolar / Q000187:drug effects; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; C079053:IH 764-3; C052656:bleomycin A6; D001761:Bleomycin