Amino acid sequence of human pregnancy-associated plasma protein-A derived from cloned cDNA.
The amino acid sequence of human pregnancy-associated plasma protein-A (PAPP-A), a component of the circulating complex with the proform of eosinophil major basic protein (proMBP), has been determined from partial protein sequencing and from sequencing of cloned cDNA. The PAPP-A monomer contains 1547 amino acid residues, but is derived from a larger precursor of placental origin. PAPP-A contains 82 Cys residues, which are all bridged, 14 putative sites for N-glycosylation, and 7 putative sites for attachment of glycosaminoglycan groups. The C-terminal part of PAPP-A contains 5 approximately 60-residue motifs related to the short consensus repeats of complement proteins and selectins. The SCRs presently known can be grouped into three classes: complement-type, class I; selectin-type, class II; PAPP-A-type, class III. PAPP-A further contains three approximately 26-residue motifs, related to the lin-notch motifs of proteins regulating early tissue differentiation, and, in addition, a putative Zn2+ binding site similar to that found in many metalloproteinases has been identified. Apart from these features, the PAPP-A sequence is not related to other known protein sequences.
Kristensen
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T
Department of Chemistry, Aarhus University, Denmark.
Oxvig
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C
Sand
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O
Møller
N P
NP
Sottrup-Jensen
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L
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003062:Codon; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000737:chemistry*; D005260:Female; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D011247:Pregnancy; D011266:Pregnancy-Associated Plasma Protein-A / Q000737:chemistry* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012091:Repetitive Sequences, Nucleic Acid; D014018:Tissue Distribution; D015032:Zinc / Q000378:metabolism
D003062:Codon; D018076:DNA, Complementary; D012333:RNA, Messenger; D011266:Pregnancy-Associated Plasma Protein-A; D015032:Zinc