title: Role of Ca2+ in alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-mediated polyphosphoinositide turnover in primary neuronal cultures.
abstract: Excitatory amino acid (EAA) receptors and EAA-mediated stimulation of polyphosphoinositide (poly-PI) turnover were studied in cultured neurons at different days in vitro (DIV). Six main observations have emerged from these studies: (a) Neurons increased their sensitivity to EAAs as a function of time in culture, indicated by increasing EAA-mediated poly-PI turnover. (b) Extracellular Ca2+ concentration played an important role in alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-stimulated poly-PI turnover in cells at 4 DIV, whereas poly-PI turnover mediated by L-glutamate and trans-1-amino-cyclopentane-1,3-dicarboxylic acid was not Ca(2+)-dependent. (c) A marked stimulation of poly-PI turnover by AMPA was seen in the cultured neurons at 4 DIV, but not at 17 DIV, suggesting that a distinct EAA receptor sensitive to AMPA is transiently expressed. (d) The Ca2+ ionophore A23187 increased poly-PI turnover in cultured neurons, suggesting that Ca2+ entry is involved in stimulating poly-PI turnover. (e) Stimulation of poly-PI turnover by carbachol was greater in neurons at 17 DIV as compared with 4 DIV, and appeared to be Ca(2+)-dependent across DIV. (f) 6-Cyano-7-nitroquinoxaline-2,3-dione, an antagonist for non-N-methyl-D-aspartate ionotropic EAA receptors, inhibited 100% and 35% of AMPA- and quisqualate-induced poly-PI turnover, respectively, suggesting an involvement of ionotropic AMPA/quisqualate receptors in stimulating poly-PI turnover.
authors.affiliation: Department of Physiology and Cell Biology, University of Kansas, Lawrence 66045-2106.
authors.forename: Y H
authors.initials: YH
authors.lastname: Lee
authors.forename: D L
authors.initials: DL
authors.lastname: Deupree
authors.forename: S C
authors.initials: SC
authors.lastname: Chen
authors.forename: L S
authors.initials: LS
authors.lastname: Kao
authors.forename: J Y
authors.initials: JY
authors.lastname: Wu
mesh_terms: D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D002217:Carbachol / Q000494:pharmacology; D002478:Cells, Cultured; D003035:Cobalt / Q000494:pharmacology; D005110:Extracellular Space / Q000378:metabolism; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D009474:Neurons / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D051381:Rats; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D005971:Glutamates; D018129:Phosphatidylinositol Phosphates; D000001:Calcimycin; D003035:Cobalt; D018698:Glutamic Acid; D013779:Tetrodotoxin; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D002217:Carbachol; D002118:Calcium