title: Serological and genomic characterization of equine rotavirus VP4 proteins identifies three different P serotypes.
abstract: A series of viral reassortants was prepared between equine rotaviruses H1 (G5), H2 (G3), and L338 (G13) and human rotavirus ST3 (G4). All contained the VP4 cognate gene segment 4 from the equine parental virus and the VP7 cognate gene segment 9 from ST3. Using these viruses and antisera prepared to them, it was shown that each of the three equine viruses possessed a serologically distinct VP4 or P serotype with a > or = 16-fold difference in reciprocal cross-neutralization titers. H1 VP4 was closely related to that of porcine virus OSU, i.e., P7. L338 gene 4 was sequenced, and the sequence and serological data indicated that it constituted a novel P serotype L338. P serotype H2 was predominant among our cell culture-adapted equine rotavirus strains, but showed some serological cross-reactivity.
authors.affiliation: Moredun Research Institute, Edinburgh, United Kingdom.
authors.forename: P
authors.initials: P
authors.lastname: Isa
authors.forename: D R
authors.initials: DR
authors.lastname: Snodgrass
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000145:classification* / Q000235:genetics / Q000276:immunology; D036022:Capsid Proteins; D004279:DNA, Viral; D000939:Epitopes / Q000276:immunology; D005814:Genes, Viral; D006801:Humans; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D012400:Rotavirus Infections / Q000382:microbiology; D017386:Sequence Homology, Amino Acid; D012703:Serotyping
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D000939:Epitopes; C060339:VP4 protein, Rotavirus