Cloning and characterization of a cluster of genes coding for 5S rRNA and three tRNAs from Azospirillum lipoferum.
A genomic library was constructed from a HindIII digest of Azospirillum lipoferum chromosomal DNA in the HindIII site of pUC19. From the library, a clone, pALH64, which showed strong hybridization with 3' end labeled A. lipoferum total tRNAs and which contains a 2.9 kb insert was isolated and restriction map of the insert established. The nucleotide sequence of a 490 bp HindIII-HincII subfragment containing a cluster of genes coding for 5S rRNA, tRNA(Val)(UAC), tRNA(Thr)(UGA) and tRNA(Lys)(UUU) has been determined. The gene organization is 5S rRNA (115 bp), spacer (10 bp), tRNA(Val) (76 bp), spacer (3 bp), tRNA(Thr) (76 bp), spacer (7 bp) and tRNA(Lys) (76 bp). Hybridization experiments using A. lipoferum total tRNAs and 5S rRNA with the cloned DNA probes revealed that all three tRNA genes and the 5S rRNA gene are expressed in vivo in the bacterial cells.
Jayabaskaran
C
C
Department of Biochemistry, Indian Institute of Science, Bangalore.
Sivakamasundari
R
R
D016949:Azospirillum / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009690:Nucleic Acid Conformation; D012313:RNA / Q000096:biosynthesis; D012329:RNA, Bacterial / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000235:genetics*; D012343:RNA, Transfer / Q000235:genetics*; D012357:RNA, Transfer, Lys / Q000235:genetics; D012363:RNA, Transfer, Thr / Q000235:genetics; D012366:RNA, Transfer, Val / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D012329:RNA, Bacterial; D012341:RNA, Ribosomal, 5S; D012357:RNA, Transfer, Lys; D012363:RNA, Transfer, Thr; D012366:RNA, Transfer, Val; C039919:RNA, recombinant; D012313:RNA; D012343:RNA, Transfer