title: Association of the GPI-anchored leucocyte surface glycoproteins with ganglioside GM3.
abstract: The major glycolipid co-immunopurifying with the glycosylphosphatidylinositol-anchored leucocyte surface glycoprotein CD59 from detergent lysates of human T cell lines HPB ALL, Jurkat and myeloid line HL-60 was identified as the glycosphingolipid GM3. Monoclonal antibodies to GM3 immunoprecipitated the same large detergent-resistant, protein-tyrosine kinase containing "GPI-complexes" as antibodies to several GPI-anchored proteins. Therefore GM3 is another component of these large membrane complexes potentially involved in signalling through GPI-anchored receptors or through some glycolipids.
authors.affiliation: GBF, Immunobiology Group, Braunschweig, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Kniep
authors.forename: T
authors.initials: T
authors.lastname: Cinek
authors.forename: P
authors.initials: P
authors.lastname: Angelisová
authors.forename: V
authors.initials: V
authors.lastname: Horejsí
mesh_terms: D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D018957:CD59 Antigens; D005679:G(M3) Ganglioside / Q000032:analysis / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols / Q000032:analysis / Q000378:metabolism*; D006098:Granulocytes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007163:Immunosorbent Techniques; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000737:chemistry / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018957:CD59 Antigens; D005679:G(M3) Ganglioside; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins