Cytoskeleton-associated antigens from African trypanosomes are recognized by self-reactive antibodies of uninfected mice.
Serum from uninfected mice of different strains, as well as from germ-free animals, contains antibodies which react specifically with at least two trypanosomal proteins, I/6 and MARP1. These antibody populations are highly specific for the respective proteins, are of similar affinity as hyperimmune antibodies, and consist of IgM as well as IgG isotypes. Hyperimmune antibody raised against the cross-reacting trypanosomal protein I/6 detects a 60 kDa protein in mouse 3T6 cells, which is a component of the fibroblast cytoskeleton.
Müller
N
N
Institut für Allgemeine Mikrobiologie, University of Bern, Switzerland.
Imboden
M
M
Detmer
E
E
Mansfield
J M
JM
Seebeck
T
T
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000917:Antibody Formation; D000953:Antigens, Protozoan / Q000276:immunology*; D001323:Autoantibodies / Q000276:immunology*; D003429:Cross Reactions; D003598:Cytoskeletal Proteins / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005407:Flagella / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005856:Germ-Free Life; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D051379:Mice; D008815:Mice, Inbred Strains; D008869:Microtubule-Associated Proteins / Q000276:immunology; D015800:Protozoan Proteins / Q000276:immunology; D016833:Trypanosoma brucei rhodesiense / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D001323:Autoantibodies; D003598:Cytoskeletal Proteins; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C073903:MARP-1 protein, Trypanosoma brucei; D008869:Microtubule-Associated Proteins; D015800:Protozoan Proteins