A higher plant extracellular vitronectin-like adhesion protein is related to the translational elongation factor-1 alpha.
Higher plant proteins immunologically related to the animal substrate adhesion molecule vitronectin have recently been observed and implicated in a variety of biological processes, such as plasma membrane-cell wall adhesion, pollen tube extension, and bacterium-plant interaction. We provide evidence that, similar to vitronectin, one of these proteins, PVN1 (plant vitronectin-like 1), isolated from 428 mM NaCl-adapted tobacco cells binds to glass surfaces an heparin. PVN1 was isolated by glass bead affinity chromatography. Isolated PVN1 has adhesive activity based on results from a baby hamster kidney cell-spreading assay. This plant adhesion protein was detected in all tissues examined but was most abundant in roots and salt-adapted cultured cells. Immunogold labeling indicated that PVN1 is localized in the cell wall of cortical and transmitting tissue cells of pollinated mature styles. A partial amino acid sequence of PVN1 revealed no similarity with vitronectin but, instead, was nearly identical to the translational elongation factor-1 alpha (EF-1 alpha). A clone isolated by screening a tobacco cDNA expression library with anti-PVN1 encoded a protein with greater than 93% identity to sequences of EF-1 alpha from plants of numerous species. Immunological cross-reactivity between tobacco PVN1 and EF-1 alpha as well as the reaction between the EF-1 alpha antibody and the 65- and 75-kD vitronectin-like proteins of a fucoidal alga supported the conclusion that the plant extracellular adhesion protein PVN1 is related to EF-1 alpha.
Zhu
J K
JK
Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, Indiana 47907-1165.
Damsz
B
B
Kononowicz
A K
AK
Bressan
R A
RA
Hasegawa
P M
PM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000302:isolation & purification; D002451:Cell Compartmentation; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D006023:Glycoproteins / Q000235:genetics; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000235:genetics; D000459:Phaeophyceae / Q000737:chemistry; D010940:Plant Proteins; D010947:Plants, Toxic; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000737:chemistry / Q000235:genetics*; D019096:Vitronectin
D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D006023:Glycoproteins; C087261:PVN1 protein, Nicotiana tabacum; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D010940:Plant Proteins; D019096:Vitronectin