title: Albumin-like glycoprotein from human fetal tissue.
abstract: Albumin-like glycoprotein (Gp66) with a molecular mass of 66 kDa has been isolated from human fetal tissue by size-exclusion, ion-exchange chromatography and reverse-phase HPLC. Reactivity of Gp66 with antiserum raised against the major protein components fraction of human fetal serum was observed. The N-terminal 35 amino acid residues of Gp66 were identical to human serum albumin. Meanwhile Gp66 differed from albumin by a/ the presence of 3-5 Trp residues instead of 1 according to fluorescence and UV-spectra, b/ the glycosylation pattern: bi-, tri-, and tetraantennary sialooligosaccharides of a complex type were present. Isoelectric focusing revealed 4 isoforms (pI ranging within 4.8 to 5.1) of Gp66. Gp66 (but not asialo-Gp66) was able to inhibit the cytotoxic effect of TNF against the tumor cell line L929. Inhibition of WEHI-3 and L929 tumor cells proliferation by Gp66 was similar to that of albumin.
authors.affiliation: Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: A A
authors.initials: AA
authors.lastname: Karelin
authors.forename: M M
authors.initials: MM
authors.lastname: Phylippova
authors.lastname: Blishchenko EYu
authors.forename: N V
authors.initials: NV
authors.lastname: Bovin
authors.forename: V V
authors.initials: VV
authors.lastname: Nasonov
authors.forename: S D
authors.initials: SD
authors.lastname: Shiyan
authors.forename: E E
authors.initials: EE
authors.lastname: Petrova
authors.forename: V A
authors.initials: VA
authors.lastname: Nesmeyanov
authors.forename: L P
authors.initials: LP
authors.lastname: Sashchenko
authors.forename: N V
authors.initials: NV
authors.lastname: Gnuchev
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012709:Serum Albumin / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000633:toxicity; D019096:Vitronectin
chemical_list: D006023:Glycoproteins; D012709:Serum Albumin; D014409:Tumor Necrosis Factor-alpha; D019096:Vitronectin