Interleukin-10 inhibits B7 and intercellular adhesion molecule-1 expression on human monocytes.
There is evidence that interleukin -10(IL-10) interferes with the costimulatory properties of antigen-presenting cells and, thereby, inhibits their ability to induce T cell activation. To determine whether this effect might involve modulation of the expression of accessory molecules, we analyzed by flow cytometry the influence of human IL-10 on the basal expression of intercellular adhesion molecule 1 (ICAM-1) as well as on the interferon gamma (IFN-gamma)-induced up-regulation of ICAM-1 and B7 at the surface of human monocytes. IL-10 inhibited both the basal expression and the IFN-gamma-induced ICAM-1 up-regulation. IL-10 also reduced B7 up-regulation on IFN-gamma-stimulated monocytes. The inhibitory effect of IL-10 both on ICAM-1 and B7 expression was shown to be dose dependent. We conclude that the ability of IL-10 to decrease both ICAM-1 and B7 expression on monocytes might contribute to its immunosuppressive properties.
Willems
F
F
Department of Immunology, Hôpital Erasme, Université Libre de Bruxelles, Belgium.
Marchant
A
A
Delville
J P
JP
Gérard
C
C
Delvaux
A
A
Velu
T
T
de Boer
M
M
Goldman
M
M
D018122:B7-1 Antigen / Q000032:analysis*; D015815:Cell Adhesion Molecules / Q000032:analysis*; D002478:Cells, Cultured; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma / Q000494:pharmacology; D016753:Interleukin-10 / Q000494:pharmacology*; D009000:Monocytes / Q000737:chemistry / Q000187:drug effects*; D011994:Recombinant Proteins / Q000494:pharmacology
D018122:B7-1 Antigen; D015815:Cell Adhesion Molecules; D006684:HLA-DR Antigens; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1; D016753:Interleukin-10; D007371:Interferon-gamma