Substance P receptors on human astrocytoma cells are linked to glycogen breakdown.
In this study we report that substance P stimulated [3H]glycogen breakdown and elevation of intracellular Ca2+ concentration in the human astrocytoma cell line UC-11MG. Both effects were dose dependent, and completely blocked by CP-96,345 suggesting the involvement of an NK1 receptor. Our previous studies indicated that norepinephrine and histamine stimulate glycogenolysis via cAMP and Ca2+ respectively. Combined stimulation with substance P and norepinephrine or histamine resulted in additive effects suggesting that there is no interaction between these neurotransmitters in regulating glycogenolysis in these cells. These results confirm that UC-11MG cells are a useful model system to investigate the functional role of neurotransmitter receptors in astroglial cells.
Medrano
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S
Department of Emergency Medicine, University of Cincinnati, College of Medicine, OH 45267.
Gruenstein
E
E
Dimlich
R V
RV
D001254:Astrocytoma / Q000378:metabolism* / Q000473:pathology; D001713:Biphenyl Compounds / Q000494:pharmacology; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D006003:Glycogen / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology; D006801:Humans; D009638:Norepinephrine / Q000494:pharmacology; D018040:Receptors, Neurokinin-1 / Q000378:metabolism*; D013373:Substance P / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D001713:Biphenyl Compounds; D004338:Drug Combinations; D018040:Receptors, Neurokinin-1; D013373:Substance P; D006632:Histamine; D006003:Glycogen; C066892:CP 96345; D009638:Norepinephrine