GLUT-3 (brain-type) glucose transporter polypeptides in human blood platelets.
Antiserum directed against a C-terminal peptide of the human GLUT-3 (brain type) equilibrative glucose transporter isoform reacted with polypeptides M(r) 46,000-48,000 on immunoblots of human platelets. Photoirradiation of human platelets in the presence of 3H-cytochalasin B led to radiolabeling of polypeptides of identical mobility. This labeling was substantially reduced by preincubation the cells with 440 mM D-glucose, but not 440 mM L-glucose, consistent with glucose transporter function. Only traces of GLUT-1 (erythrocyte type) glucose transporter polypeptides were detected, and there was no evidence of GLUT-2 (liver type) transporter on immunoblots of platelet proteins.
Craik
J D
JD
Chemistry Department, Bishop's University, Lennoxville, Quebec, Canada.
Stewart
M
M
Cheeseman
C I
CI
D000818:Animals; D000918:Antibody Specificity; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism*; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D001923:Brain Chemistry / Q000502:physiology*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000737:chemistry / Q000378:metabolism; D051274:Glucose Transporter Type 3; D006801:Humans; D066298:In Vitro Techniques; D009004:Monosaccharide Transport Proteins / Q000097:blood* / Q000737:chemistry; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism*; D010455:Peptides / Q000097:blood*; D051381:Rats; D012016:Reference Values
D001798:Blood Proteins; D051274:Glucose Transporter Type 3; D009004:Monosaccharide Transport Proteins; D009419:Nerve Tissue Proteins; D010455:Peptides; C495687:SLC2A3 protein, human; C495690:Slc2a3 protein, rat