Expression of the linear DNA plasmid pRS64 in the plant pathogenic fungus Rhizoctonia solani.
The plant pathogenic isolate RI-64 of anastomosis group 4 of Rhizoctonia solani possesses three linear DNA plasmids (pRS64-1, -2, and -3). Unique poly(A)- RNA, 0.5 kb in length and hybridizable with the pRS64 DNAs was found in mycelial cells of the isolate RI-64. The overall homology at the nucleotide level between pRS64-1, -2, and -3, and the cDNA prepared from the poly(A)- RNA was 100%, 73%, and 84%, respectively. The open reading frames found in pRS64-1, -2, and -3 (ORF1-1, ORF2-1, and ORF3-1) are 68 amino acids long. The amino acids sequence showed no significant homology with known proteins. Extracts from Escherichia coli cells expressing ORF1-1 contain a specific protein of 7 kDa. Antisera raised against the ORF1-1 product obtained from E. coli cells cross-reacted with the specific proteins found in the mycelia. The results indicate that the DNA plasmids found in R. solani contain a sequence that encodes a specific protein which may be involved in determination of plant pathogenicity.
Hongo
M
M
Miyasaka
A
A
Suzuki
F
F
Hashiba
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000096:biosynthesis*; D004926:Escherichia coli; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames; D010455:Peptides / Q000235:genetics; D010957:Plasmids; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012232:Rhizoctonia / Q000235:genetics*
D004271:DNA, Fungal; D010455:Peptides; D011061:Poly A; D012313:RNA