title: Expression of intercellular adhesion molecule-1 during inner ear inflammation.
abstract: This study was designed to investigate the expression of intercellular adhesion molecule-1 (ICAM-1) on the spiral modiolar vein (SMV) with its collecting venules (CVs) and the venules of the endolymphatic sac during inner ear inflammation. These data will further elucidate the role of adhesion molecules in extravasation of inflammatory cells from blood vessels during an inner ear immune response. Labyrinthitis was induced in rats by inoculation of keyhole limpet hemocyanin into the scala tympani of animals who had been systemically sensitized to it. Expression of ICAM-1 was examined with a mouse monoclonal antibody to rat ICAM-1 by immunohistochemistry. ICAM-1 was found weakly on the epithelium of SMVs and CVs as early as 6 hours postchallenge, reaching a maximum by day 2 and then fading away gradually. The maximum influx of immunocompetent cells into the cochlea was seen between days 3 and 7. Staining for ICAM-1 was observed on the epithelium of the endolymphatic sac and perisaccular region at 12 and 24 hours, respectively, and this was associated with infiltration of cells into these areas 3 days postchallenge. By day 28, the inner ear had developed endolymphatic hydrops, but at this time it showed almost no significant staining with anti-ICAM-1. The molecule was also expressed in the mesothelium of perilymph, the perineurium of cochlear nerves, the spiral ligament, and the basal cells of the stria vascularis following immunization. Our data provide evidence that endothelial cells of the SMV and its CVs, as well as other inner sites, have the potential to express ICAM-1. This expression precedes the influx of immune cells; therefore, it is possible that this ligand plays a pivotal role in the onset of inflammation in the inner ear. This study also confirmed that the immune response results in endolymphatic hydrops as a long-term consequence.
authors.affiliation: Division of Otolaryngology-Head and Neck Surgery, University of California, San Diego.
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: J P
authors.initials: JP
authors.lastname: Harris
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D004712:Endolymphatic Sac / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D004730:Endothelium, Vascular / Q000378:metabolism / Q000473:pathology; D005260:Female; D007108:Immune Tolerance; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007762:Labyrinthitis / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011917:Rats, Inbred Lew; D013194:Staining and Labeling; D014680:Veins / Q000378:metabolism / Q000473:pathology; D014699:Venules / Q000378:metabolism / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D018799:Intercellular Adhesion Molecule-1