The effect of ethanol and extracellular matrix on induction of p36 protein kinase substrate expression in rat hepatocytes.
p36 plays a direct role in DNA synthesis and is overexpressed in transformed cells. It is also an important component linking the cell membrane to intracellular cytoskeletal components. Experiments were performed in primary rat hepatocyte culture stimulated with epidermal growth factor (EGF) to determine if p36 expression was related to DNA synthesis or to the effect of the extracellular matrix on hepatocyte differentiation; ethanol was employed as an agent to inhibit hormone stimulated hepatocyte DNA synthesis. It was found that hepatocyte p36 expression was highly dependent on the type of extracellular matrix and the time in culture. There was no correlation of p36 expression with DNA synthesis and, therefore, p36 levels appeared more closely related to the differentiated phenotype, induced by the extracellular matrix interactions rather than cellular proliferation.
Bhavani
K
K
Molecular Hepatology Laboratory, Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown 02129.
Brown
N V
NV
Carlson
R I
RI
Rhoads
D
D
Wands
J R
JR
D000818:Animals; D017306:Annexin A2 / Q000096:biosynthesis*; D015152:Blotting, Northern; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D004338:Drug Combinations; D004815:Epidermal Growth Factor / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000502:physiology*; D005260:Female; D007700:Kinetics; D007797:Laminin / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism*; D011509:Proteoglycans / Q000494:pharmacology; D012313:RNA / Q000032:analysis; D051381:Rats; D013379:Substrate Specificity; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D017306:Annexin A2; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D000431:Ethanol; D004815:Epidermal Growth Factor; D012313:RNA; D003094:Collagen; D004247:DNA; D011494:Protein Kinases; D013936:Thymidine