Detection of tumor-specific antigens in Philadelphia chromosome positive leukemias.
In chronic myeloid leukemia (CML) and acute lymphoblastic leukemia (ALL) the Ph1 chromosome (22q-) is the most frequent chromosomal aberration encountered. At the molecular level the c-abl gene from chr. 9 is translocated to the bcr gene on chr. 22. As a result, a chimeric bcr-abl gene is generated, which encodes chimeric proteins. Since these proteins are only expressed in Ph1 positive cells, they are per definition tumor-specific. In this report we describe the reactivity of polyvalent antisera raised against synthetic peptides corresponding to the tumor-specific bcr-abl junctions. Native chimeric proteins were specifically recognized by these junction-specific antisera. Therefore we conclude that the bcr-abl junctions are antigenically exposed on the chimeric proteins. We discuss the relevance of these antisera for CML and ALL diagnosis.
van Denderen
J
J
Department of Immunology, Erasmus University, Rotterdam, The Netherlands.
ten Hacken
P
P
Berendes
P
P
van Ewijk
W
W
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D016044:Fusion Proteins, bcr-abl / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D016313:Genes, abl; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D010677:Philadelphia Chromosome; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000145:classification / Q000175:diagnosis / Q000235:genetics / Q000276:immunology*; D012680:Sensitivity and Specificity
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D000939:Epitopes; D010446:Peptide Fragments; D016044:Fusion Proteins, bcr-abl