title: Loss and gain of chromosomes 1, 18, and Y in prostate cancer.
abstract: Nuclear suspensions of 42 prostate carcinoma specimens obtained at surgery were used to investigate loss and gain chromosomes 1, 18, and Y by fluorescence in situ hybridization (FISH) with centromere-specific probes. The outcome of FISH analysis was correlated with clinical parameters and the relationship between DNA-FCM (ploidy at cellular level) and FISH (ploidy of individual chromosomes) was assessed. Significant loss of chromosomes 1 and 18 was infrequent (respectively, three and five cases), but 53% of the tested specimens showed loss of Y. Loss was not correlated with DNA ploidy. Significant gain occurred in 36% (chromosome 1), 63% (chromosome 18), and 28% (Y) of the specimens. Gain of chromosome 18 was shown in DNA diploid (7/14) and aneuploid tumors (18/26), while gain of chromosomes 1 and Y was nearly restricted to DNA aneuploid specimens. Significant unbalance between these chromosomes occurred in 11 cases. Most cases which had significant gain of chromosome 1 or 18 showed trisomic as well as tetrasomic cells. Simultaneous loss of some and gain of other investigated chromosomes is suggestive of clonal heterogeneity and/or multiclonality. This was observed in eight tumors. Correlation between DNA-FCM and FISH was best for the Y chromosome. DNA-FCM showed more aberrant histograms with increasing stage and grade of tumors. The presence of numerical aberrations of the investigated chromosomes however, seemed independent of clinical grade or stage.
authors.affiliation: Department of Urology, Erasmus University, Rotterdam, The Netherlands.
authors.forename: J J
authors.initials: JJ
authors.lastname: König
authors.forename: W
authors.initials: W
authors.lastname: Teubel
authors.forename: J W
authors.initials: JW
authors.lastname: van Dongen
authors.forename: J C
authors.initials: JC
authors.lastname: Romijn
authors.forename: A
authors.initials: A
authors.lastname: Hagemeijer
authors.forename: F H
authors.initials: FH
authors.lastname: Schröder
mesh_terms: D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1; D002887:Chromosomes, Human, Pair 18; D004273:DNA, Neoplasm; D006801:Humans; D008297:Male; D011003:Ploidies; D011470:Prostatic Hyperplasia / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics*; D014998:Y Chromosome
chemical_list: D004273:DNA, Neoplasm