Transformation of NIH3T3 cells with ras oncogenes abrogates the retinoic acid induction of tissue transglutaminase.
Retinoic acid greatly increases enzyme activity and mRNA expression of the tissue-type transglutaminase enzyme in NIH3T3 cells. This response is blocked in cells transformed with activated H-ras, K-ras or N-ras oncogenes, but not in pSVneo vector transfected cells. Lack of induction by RA of the tissue-type TGase in these ras-transformed fibroblasts suggests intersecting pathways between retinoid action and the ras oncogene.
Kosa
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Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, Maryland 20892.
Meyers
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K
De Luca
L M
LM
D016475:3T3 Cells; D000818:Animals; D015152:Blotting, Northern; D002471:Cell Transformation, Neoplastic; D003001:Cloning, Molecular; D004790:Enzyme Induction; D015870:Gene Expression; D011905:Genes, ras; D005822:Genetic Vectors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis; D007700:Kinetics; D007708:Kirsten murine sarcoma virus; D051379:Mice; D011061:Poly A / Q000032:analysis; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection; D011503:Transglutaminases / Q000096:biosynthesis*; D014212:Tretinoin / Q000494:pharmacology*
D012333:RNA, Messenger; D011061:Poly A; D014212:Tretinoin; D012313:RNA; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D011503:Transglutaminases