title: Hypertonic saline/dextran for cardiopulmonary bypass reduces gut tissue water but does not improve mucosal perfusion.
abstract: Gut mucosal ischemia has been associated with cardiopulmonary bypass (CPB) and may contribute to postoperative systemic inflammatory response and multiorgan dysfunction. Hypertonic saline/dextran (HSD) has been previously shown to selectively increase mucosal blood flow in circulatory shock. To determine whether adding HSD to the prime solution for CPB improves gut mucosal blood flow and oxygenation, we performed normothermic, non-cross-clamped CPB in pigs with 1 ml/kg of HSD (25% NaCl/24% dextran 70) (HSD group, n = 9) or lactated Ringer's solution (LRS group, n = 9) as control added to a standard prime. Animals were instrumented with ultrasonic flow probes on the superior mesenteric artery (SMA), laser Doppler mucosal flow probes in the ileum, and indwelling portal vein catheters and tonometers for mucosal hydrogen ion measurements and pH calculations in the stomach, ileum, and rectum. The total infused volume and net fluid balance was significantly lower in the HSD than in the LRS group (649 +/- 171 ml vs 2075 +/- 385 ml and 502 +/- 182 ml vs 1891 +/- 363 ml, respectively, P < 0.01). SMA flow in the LRS group increased to 110-123% of baseline during CPB and was significantly higher than that in the HSD group which remained unchanged. Ileal mucosal blood flow decreased significantly to 70-50% of baseline in both groups with no difference between groups. Gut oxygen (O2) delivery decreased during CPB in both groups, but O2 consumption remained unchanged. Gastric, ileal, and rectal mucosal pH decreased progressively, and portal venous blood pH also decreased in both groups, but there was no significant difference between groups.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, University of Texas Medical Branch, Galveston 75555-0528.
authors.forename: W
authors.initials: W
authors.lastname: Tao
authors.forename: J B
authors.initials: JB
authors.lastname: Zwischenberger
authors.forename: T T
authors.initials: TT
authors.lastname: Nguyen
authors.forename: R A
authors.initials: RA
authors.lastname: Vertrees
authors.forename: L K
authors.initials: LK
authors.lastname: Nutt
authors.forename: L B
authors.initials: LB
authors.lastname: McDaniel
authors.forename: G C
authors.initials: GC
authors.lastname: Kramer
mesh_terms: D000818:Animals; D001834:Body Water; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D003911:Dextrans / Q000627:therapeutic use*; D004064:Digestive System / Q000098:blood supply* / Q000737:chemistry* / Q000503:physiopathology; D004386:Duodenum / Q000098:blood supply / Q000737:chemistry / Q000503:physiopathology; D005260:Female; D006863:Hydrogen-Ion Concentration; D006982:Hypertonic Solutions; D007082:Ileum / Q000098:blood supply / Q000737:chemistry / Q000503:physiopathology; D007413:Intestinal Mucosa / Q000098:blood supply / Q000737:chemistry / Q000503:physiopathology*; D007511:Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D007583:Jejunum / Q000098:blood supply / Q000737:chemistry / Q000503:physiopathology; D010101:Oxygen Consumption / Q000502:physiology; D012007:Rectum / Q000098:blood supply / Q000737:chemistry / Q000503:physiopathology; D012039:Regional Blood Flow; D012965:Sodium Chloride / Q000627:therapeutic use*; D013270:Stomach / Q000098:blood supply / Q000737:chemistry / Q000503:physiopathology; D013552:Swine
chemical_list: D003911:Dextrans; D006982:Hypertonic Solutions; D012965:Sodium Chloride