Genotoxicity testing of five compounds in three Drosophila short-term somatic assays.
To provide further background data for the somatic mutation and/or recombination tests in Drosophila melanogaster, we have evaluated the response in 3 assays (zeste-white, white-ivory and wing spot) of 5 chemicals classified by the U.S. National Toxicology Program (NTP) as genotoxic non-carcinogens (or ambiguous). The selected compounds were 2-chloromethylpyridine, 1-nitronaphthalene, 4-nitro-o-phenylenediamine, 3-nitropropionic acid and p-phenylenediamine. Our results show that all the compounds tested produce significant increases in the frequency of mutant clones, in at least one of the assays, p-phenylenediamine being the compound which presents a clearer mutagenic activity, and the wing spot test, the assay that detects more genotoxic compounds (4/5).
Batiste-Alentorn
M
M
Departament de Genètica i de Microbiologia, Facultat de Ciències, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Xamena
N
N
Creus
A
A
Marcos
R
R
D000818:Animals; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D005260:Female; D008297:Male; D009152:Mutagenicity Tests / Q000592:standards*; D009153:Mutagens / Q000633:toxicity*; D009281:Naphthalenes / Q000633:toxicity; D009574:Nitro Compounds; D010655:Phenylenediamines / Q000633:toxicity; D010847:Picolines / Q000633:toxicity*; D011422:Propionates / Q000633:toxicity
D009153:Mutagens; D009281:Naphthalenes; D009574:Nitro Compounds; D010655:Phenylenediamines; D010847:Picolines; D011422:Propionates; C091423:2-chloromethylpyridine; C012277:1,2-diamino-4-nitrobenzene; C016614:1-nitronaphthalene; C015392:3-nitropropionic acid; C029728:4-phenylenediamine