[Single channel analysis on calcium channel blockade action of panaxadiol and panaxatriol saponins on cultured rat ventricular myocytes].
Wistar rat ventricular myocytes were isolated. Panaxadiol saponins 1500 micrograms.ml-1, panaxatriol saponins 300 micrograms.ml-1, verapamil 37.5 micrograms.ml-1, or BAY k 8644 5 mumol.L-1 were added into the bath solution separately. The single channel activities of L, T, and B type calcium channels were recorded before and after the administration, using voltage patch-clamp technique in cell-attached configuration. The calcium channel blockade effect of these 2 groups of ginsenosides was authenticated verified. The mechanism existed in the decrease in both the open time and the open-state probability of the calcium channel.
Zhang
W J
WJ
Department of Physiology, Norman Bethune University of Medical Sciences, Changchun, China.
Zhong
G G
GG
Jiang
Y
Y
Wang
X M
XM
Wang
Z F
ZF
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D015220:Calcium Channels / Q000187:drug effects*; D002478:Cells, Cultured; D036145:Ginsenosides; D009206:Myocardium / Q000166:cytology*; D005894:Panax; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D014315:Triterpenes / Q000494:pharmacology*; D014700:Verapamil / Q000494:pharmacology
D015220:Calcium Channels; D036145:Ginsenosides; D014315:Triterpenes; C004564:panaxadiol; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; C004563:panaxatriol; D014700:Verapamil