title: Mutagenicity of ethanolic extracts of used acrylic dentures.
abstract: The in vivo physicochemical sorption of mutagenic substances onto acrylic polymers was investigated in worn acrylic dentures. Thus, ethanolic extracts of acrylic dentures from 41 of a total of 69 human donors (60%), were found mutagenic in the standard plate incorporation Salmonella mutagenicity test against either TA98 or TA100 strains. Denture extracts from smokers produced mutagenicity more often than the ones from non-smokers (75% vs. 45%, P 0.01). Mutagenicity was preferentially directed against TA98 (TA98:TA100 = 2.9:1, P < 0.0005). Predilection for TA98 was more pronounced in denture extracts from non-smokers (4.7:1) than from smokers (2.0:1). When direct mutagenicity was observed, it was reduced by the rat-liver S9. Induced mutant yields were 6.1 +/- 3.9 and 7.0 +/- 8.9 times higher than the spontaneous for TA98 and TA100 respectively (smokers, 50-cm2 denture surface area eq./plate+S9). Denture extracts from smokers induced higher levels of mutation than the ones from non-smokers (TA98 + S9, smoker:non-smoker = 2:1, P < 0.01). Mutagenicity was associated with longer periods of denture usage (P 0.007). Thus, denture poly(methyl methacrylate) base material can adsorb mutagenic substances, possibly from diet and tobacco, which are extractable by ethanol. Theoretically, the in situ alcoholic desorption and recirculation of carcinogenic mutagens may have a contributory role in certain cases of intra-oral and upper alimentary tract carcinogenesis.
authors.affiliation: Department of Periodontics, Biochemistry and Microbiology, Loyola University, School of Dentistry, Maywood, IL.
authors.forename: D M
authors.initials: DM
authors.lastname: Parisis
authors.forename: N L
authors.initials: NL
authors.lastname: Eskoz
authors.forename: W G
authors.initials: WG
authors.lastname: Henderson
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D003069:Coffee; D003764:Dental Materials / Q000737:chemistry*; D003778:Dentures; D000431:Ethanol; D006801:Humans; D009152:Mutagenicity Tests; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D012907:Smoking; D013662:Tea
chemical_list: D000180:Acrylic Resins; D003069:Coffee; D003764:Dental Materials; D009153:Mutagens; D013662:Tea; D000431:Ethanol