A simple method for isolating alpha 2 macroglobulin-cytokine complexes.
There is the need for a simple, effective procedure for separating alpha 2 macroglobulin-cytokine complexes from free cytokine in order that the nature and possible immunological significance of cytokine-binding by alpha 2 macroglobulin (alpha 2-M) might be further investigated. This presentation describes a method which exploits the presence of zinc-binding sites on alpha 2-M which permit the isolation of complexes from other proteins by zinc-affinity chromatography. Furthermore the method may be used in either a column or batch format.
Cunningham
A J
AJ
Department of Surgery (WGH), University of Edinburgh Medical School, UK.
Elliott
S F
SF
Black
J R
JR
James
K
K
D002846:Chromatography, Affinity / Q000379:methods*; D016207:Cytokines / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D015203:Reproducibility of Results; D012685:Sepharose; D015032:Zinc; D000511:alpha-Macroglobulins / Q000302:isolation & purification* / Q000378:metabolism
D016207:Cytokines; D000511:alpha-Macroglobulins; D012685:Sepharose; D015032:Zinc