title: Induction of surfactin production in Bacillus subtilis by gsp, a gene located upstream of the gramicidin S operon in Bacillus brevis.
abstract: The deduced amino acid sequence of the gsp gene, located upstream of the 5' end of the gramicidin S operon (grs operon) in Bacillus brevis, showed a high degree of similarity to the sfp gene product, which is located downstream of the srfA operon in B. subtilis. The gsp gene complemented in trans a defect in the sfp gene (sfpO) and promoted production of the lipopeptide antibiotic surfactin. The functional homology of Gsp and Sfp and the sequence similarity of these two proteins to EntD suggest that the three proteins represent a new class of proteins involved in peptide secretion, in support of a hypothesis published previously (T. H. Grossman, M. Tuckman, S. Ellestad, and M. S. Osburne, J. Bacteriol. 175:6203-6211, 1993).
authors.affiliation: Fachbereich Chemie/Biochemie, Philipps-Universität Marburg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Borchert
authors.forename: T
authors.initials: T
authors.lastname: Stachelhaus
authors.forename: M A
authors.initials: MA
authors.lastname: Marahiel
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics*; D001483:Base Sequence; D015870:Gene Expression / Q000502:physiology; D005798:Genes, Bacterial / Q000502:physiology*; D005816:Genetic Complementation Test; D006096:Gramicidin / Q000096:biosynthesis; D055666:Lipopeptides; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D010456:Peptides, Cyclic; D017385:Sequence Homology; D017855:Transferases (Other Substituted Phosphate Groups)
chemical_list: D001426:Bacterial Proteins; D055666:Lipopeptides; D010456:Peptides, Cyclic; C082662:phosphopantetheinyl transferase; D006096:Gramicidin; C009365:surfactin peptide; D017855:Transferases (Other Substituted Phosphate Groups)