Immunohistochemical analysis of tissues regenerated from within periodontal defects treated with expanded polytetrafluoroethylene membranes.
Immunocytochemical analysis was carried out on samples of 5-, 6-, and 9-week old regenerated soft tissue taken from healing periodontal defects treated by guided tissue regeneration using expanded polytetrafluorethylene (ePTFE) membranes. A panel of monoclonal and polyconal antibodies to cytokeratins, vimentin, and collagen was used to label cells and collagen types I, III, and IV. Epithelium was identified in 7 of the 9 samples examined, in addition to mesenchymal cells staining positively for vimentin and co-distribution of collagen types I, III, and IV in all samples. Clinical observations indicated that exposure of the ePTFE membranes during healing was a frequent occurrence, and the presence and quantity of epithelium found within the healing defect beneath the membrane may be related to the extent to which this occurs.
Pritlove-Carson
S
S
Department of Periodontology, United Medical School, Guy's Hospital, London, UK.
Palmer
R M
RM
Floyd
P D
PD
Morgan
P M
PM
D000328:Adult; D003094:Collagen / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D016557:Guided Tissue Regeneration, Periodontal; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D010510:Periodontal Diseases / Q000378:metabolism*; D011138:Polytetrafluoroethylene; D014746:Vimentin / Q000378:metabolism
D014746:Vimentin; D007633:Keratins; D005340:Fibrinogen; D011138:Polytetrafluoroethylene; D003094:Collagen