title: Production of hematopoietic stem cell-chemotactic factor by bone marrow stromal cells.
abstract: Chemotactic factors produced by stromal cells in the bone marrow are characterized. Two kinds of factors produced by stromal cell lines are identified using blind-well Boyden's Chambers; one is a neutrophil-chemotactic factor and the other a hematopoietic stem cell (HSC)-chemotactic factor. The latter attracts blastic cells in a low-density fraction, which are Thy1lo, wheat germ agglutinin (WGA)hi, H-2Khi, Ly-1-, Ly-2-, L3T4-, Ly5-, and slg-. The molecular weight of this HSC-chemotactic factor is estimated to be more than 200 kD. Putative cytokines and growth factors, such as granulocyte colony-stimulating factor (CSF), macrophage CSF, granulocyte-macrophage CSF, stem cell factor (SCF), interleukin (IL)-6, and IL-3, do not possess HSC-chemotactic activity. These findings strongly suggest that bone marrow stromal cells produce a new factor that attracts HSCs.
authors.affiliation: Department of Pathology, Kansai Medical University, Osaka, Japan.
authors.lastname: Cherry
authors.forename: R
authors.initials: R
authors.lastname: Yasumizu
authors.forename: J
authors.initials: J
authors.lastname: Toki
authors.forename: H
authors.initials: H
authors.lastname: Asou
authors.forename: T
authors.initials: T
authors.lastname: Nishino
authors.forename: Y
authors.initials: Y
authors.lastname: Komatsu
authors.forename: S
authors.initials: S
authors.lastname: Ikehara
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis / Q000096:biosynthesis; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells; D002460:Cell Line; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000032:analysis / Q000096:biosynthesis*; D002633:Chemotaxis / Q000187:drug effects; D003114:Colony-Forming Units Assay; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000494:pharmacology*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D007700:Kinetics; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008809:Mice, Inbred C3H; D011994:Recombinant Proteins / Q000494:pharmacology; D019089:Stem Cell Factor; D018800:Thy-1 Antigens
chemical_list: D000954:Antigens, Surface; D002630:Chemotactic Factors; D017077:Culture Media, Conditioned; D016207:Cytokines; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D018800:Thy-1 Antigens; C086022:hematopoietic stem cell-chemotactic factor; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor