[Activity of enzymes of xenobiotics metabolism in peripheral blood lymphocytes in man].
The activity of NADPH-cytochrome c-reductase, benzpyrene hydroxylase, epoxy-hydratase and glutathione-S-transferase in human peripheral blood lymphocytes was studied. In the presence of NADPH, native lymphocytes were unable to reduce cytochrome c. In order to improve the availability of substrates for enzymes, lymphocytes were degraded by single-stage freezing-melting. At the same time, the activities of NADPH-cytochrome c-reductase, epoxide hydratase, and glutathione-S-transferase were 1.7 +/- 0.6, 49.0 +/- 18.0, and 30.0 +/- 6.0 nmol/min per mg protein, respectively. The lymphocytic levels of cytochrome P-450 were approximately 0.1-0.2 nmol per mg microsomal protein, while those of cytochrome b5 were nearly 0.5 nmol/mg microsomal protein in the lymphocytes.
Reshetniak
V I
VI
Sheremet
O K
OK
Fedorova
L V
LV
Izotov
M V
MV
Devichenskiĭ
V M
VM
D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D015786:Cytochromes b5 / Q000378:metabolism; D004851:Epoxide Hydrolases / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D009245:NADH Dehydrogenase / Q000378:metabolism; D015262:Xenobiotics / Q000378:metabolism*
D015262:Xenobiotics; D015786:Cytochromes b5; D003577:Cytochrome P-450 Enzyme System; D001579:Benzopyrene Hydroxylase; D009245:NADH Dehydrogenase; D005982:Glutathione Transferase; D004851:Epoxide Hydrolases