Airborne dissemination of Burkholderia (Pseudomonas) cepacia from adult patients with cystic fibrosis.
BACKGROUND
Burkholderia (Pseudomonas) cepacia is an increasingly important pathogen in patients with cystic fibrosis but it is unclear how it spreads from patient to patient. A study was undertaken to determine whether B cepacia could be recovered from room air occupied by colonised adult patients with cystic fibrosis.


METHODS
Air samples were obtained consecutively from an enclosed room or isolation cubicle before, during, and after occupation by six patients on nine occasions using a surface air sampler incorporating contact plates with selective medium. Settle plates were also used and sputum from five patients was cultured.


RESULTS
B cepacia was recovered from room air during occupation by five of six patients, the number of bacteria ranging from 1 to 158 cfu/m3 (mean 32 cfu/m3). The number of bacteria isolated was greater when patients were coughing. B cepacia persisted in room air on four occasions after the patient left the room, on one occasion for up to 45 minutes.


CONCLUSIONS
The isolation of B cepacia from the air of rooms occupied by colonised patients suggests that dissemination might occur by aerosol as well as by direct physical contact with patients or contaminated environmental sites.
Humphreys
H
H
Department of Microbiology/Public Health Laboratory, University Hospital Nottingham.
Peckham
D
D
Patel
P
P
Knox
A
A
D000328:Adult; D000391:Air Microbiology; D016956:Burkholderia cepacia / Q000302:isolation & purification*; D003371:Cough / Q000150:complications; D003428:Cross Infection / Q000635:transmission; D003550:Cystic Fibrosis / Q000382:microbiology*; D018562:Disease Transmission, Infectious; D005260:Female; D006801:Humans; D008297:Male; D009894:Opportunistic Infections / Q000635:transmission*; D011552:Pseudomonas Infections / Q000635:transmission*; D013183:Sputum / Q000382:microbiology