Effects of prostaglandin E2 on the micronucleus formation in mouse bone marrow cells by various mutagens.
The effects of prostaglandin E2 (PGE2), as a trigger of erythroid progenitor cells into the cell cycle, were studied on the induction of micronuclei by various mutagens; with mitomicin C (MMC) the optimal protocol was established. PGE2 itself did not induce any micronuclei in this experiment. The highest frequency of micronuclei and dose-response relationship between PGE2 doses and micronucleus frequency were observed 30 h after injection of MMC to mice administered PGE2 24 h previously. Sensitization by PGE2 pretreatment was also found for other mutagens, such as vincristine, 5-fluorouracil, benzo[a]pyrene, 1,1-dimethylhydrazine and 2-naphthylamine. These results support the hypothesis that accelerating the erythropoiesis increases the frequency of micronuclei induced by mutagens.
Suzuki
Y
Y
Department of Public Health and Environmental Medicine, Jikei University School of Medicine, Tokyo, Japan.
Shimizu
H
H
Ishikawa
T
T
Sakaba
H
H
Fukumoto
M
M
Okonogi
H
H
Kadokura
M
M
D015081:2-Naphthylamine / Q000633:toxicity; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D001772:Blood Cell Count; D001853:Bone Marrow / Q000187:drug effects* / Q000378:metabolism; D001854:Bone Marrow Cells; D004127:Dimethylhydrazines / Q000633:toxicity; D015232:Dinoprostone / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004900:Erythroblasts; D004920:Erythropoiesis / Q000187:drug effects*; D004921:Erythropoietin / Q000096:biosynthesis; D005472:Fluorouracil / Q000633:toxicity; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015162:Micronucleus Tests; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D013997:Time Factors; D014750:Vincristine / Q000633:toxicity
D004127:Dimethylhydrazines; D009153:Mutagens; D004921:Erythropoietin; D001564:Benzo(a)pyrene; C013958:dimazine; D016685:Mitomycin; D014750:Vincristine; D015081:2-Naphthylamine; D015232:Dinoprostone; D005472:Fluorouracil