title: Promoter of the mouse gene encoding calcium-independent nitric oxide synthase confers inducibility by interferon-gamma and bacterial lipopolysaccharide.
abstract: We have proved that iNOS is regulated by induction of transcription. The induction of iNOS mRNA requires synthesis of an intermediary protein(s) and probably involves action of a protein kinase or kinases. We have cloned and sequenced a 1.7-kilobase pair fragment from the 5'-flanking region of the iNOS gene and proved that this region contains a promoter which confers inducibility of iNOS by LPS and IFN-gamma.
authors.affiliation: Department of Medicine, Cornell University Medical College, New York, NY.
authors.forename: Q W
authors.initials: QW
authors.lastname: Xie
authors.forename: C
authors.initials: C
authors.lastname: Nathan
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology; D003001:Cloning, Molecular; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000235:genetics; D004790:Enzyme Induction / Q000187:drug effects; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D019001:Nitric Oxide Synthase; D011401:Promoter Regions, Genetic / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007371:Interferon-gamma; D004247:DNA; D003513:Cycloheximide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D002118:Calcium