pp60c-src expression in rat spermatogenesis.
A fractionation of the membrane extract of rat testes revealed the existence of pp60c-src kinase activity. The expression of pp60c-src was examined in the developing rat testes. The immunecomplex kinase assay using a monoclonal antibody specific to pp60c-src (mAb327) showed that the expression of pp60c-src kinase activity increased during the development of rat testes and declined in the adult. The increase in pp60c-src kinase activity observed during the development of rat testes was accompanied with an increase in the amount of pp60c-src protein. The peak period in the increase of pp60c-src kinase activity well coincided with the timing, when the spermatogenesis by meiosis just began. The immunohistochemical staining of pp60c-src in rat testes demonstrated that pp60c-src is most abundantly expressed in the spermatids which are the spermatogenic cells in the post-meiotic phase of the spermatogenesis. These findings strongly suggest that pp60c-src is a developmentally regulated gene product which is involved in rat spermatogenesis.
Nishio
H
H
First Department of Physiology, Kagawa Medical School, Japan.
Tokuda
M
M
Itano
T
T
Matsui
H
H
Takeuchi
Y
Y
Hatase
O
O
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002462:Cell Membrane / Q000201:enzymology; D015870:Gene Expression; D007150:Immunohistochemistry; D008297:Male; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis; D011505:Protein-Tyrosine Kinases / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D051381:Rats; D012741:Sexual Maturation / Q000502:physiology*; D013091:Spermatogenesis; D013737:Testis / Q000254:growth & development / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D010455:Peptides; D011505:Protein-Tyrosine Kinases; D016392:Proto-Oncogene Proteins pp60(c-src)