Preparation of metabolically labeled protein samples for quantitative analysis in SDS-polyacrylamide gel electrophoresis.
Liu
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Department of Medical Physics, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
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D000818:Animals; D001345:Autoradiography / Q000379:methods; D002250:Carbon Radioisotopes; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005347:Fibroblasts / Q000378:metabolism; D007930:Leucine / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats; D012394:Rosaniline Dyes; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling; D013462:Sulfur Radioisotopes
D002250:Carbon Radioisotopes; D011506:Proteins; D012394:Rosaniline Dyes; D013462:Sulfur Radioisotopes; D012967:Sodium Dodecyl Sulfate; C048139:Coomassie blue; D008715:Methionine; D007930:Leucine