Galanin message-associated polypeptide (GMAP) does not affect insulin secretion from isolated islets.
Galanin message-associated polypeptide (GMAP) is processed from preprogalanin. Recently, GMAP-like immunoreactivity was demonstrated in insulin cells in the endocrine pancreas. We therefore examined whether synthetic rat GMAP, like galanin, inhibits glucose-stimulated insulin secretion from isolated rat and mouse islets. We found, however, that over a wide dose range (0.1 nM to 1 microM) GMAP did not affect insulin secretion stimulated by 8.3 or 11.1 mM glucose during a 60-min incubation of single rat or mouse islets. In contrast, rat galanin, as expected, completely abolished glucose-stimulated insulin secretion at 100 nM. Thus, in contrast to galanin, GMAP does not affect insulin secretion in isolated rodent islets.
Gregersen
S
S
Department of Medicine, Lund University, Malmö, Sweden.
Langel
U
U
Bartfai
T
T
Ahrén
B
B
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D019004:Galanin; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000494:pharmacology*; D011498:Protein Precursors / Q000494:pharmacology*; D051381:Rats
D007328:Insulin; D010455:Peptides; D011498:Protein Precursors; C078371:galanin message-associated peptide; D019004:Galanin