Identification of hyaluronan binding proteins using a biotinylated hyaluronan probe.
A method for detecting hyaluronan (HA)-binding proteins in transblot assays using biotinylated HA (BHA) is described. Some of the binding characteristics of a novel HA receptor termed RHAMM (Receptor for HA-Mediated Motility) are characterized using this assay. The method is also used to detect other HA-binding proteins in tissue homogenates. This method is semiquantitative, rapid, reproducible, sensitive and therefore of potential use in identifying the levels of HA-binding proteins in different cells and tissues.
Hoare
K
K
Department of Pediatrics and Child Health, Manitoba Institute of Cell Biology, Winnipeg, Canada.
Savani
R C
RC
Wang
C
C
Yang
B
B
Turley
E A
EA
D000818:Animals; D001710:Biotin / Q000378:metabolism; D002352:Carrier Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid / Q000032:analysis; D008722:Methods; D015335:Molecular Probes / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000032:analysis*; D016201:Receptors, Lymphocyte Homing / Q000032:analysis*
D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D015335:Molecular Probes; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D001710:Biotin; D006820:Hyaluronic Acid