Use of the receptor globulin technology to search for ligands for glycosylphosphatidylinositol-linked cell surface antigens.
The ability of monoclonal antibodies to several proteins coupled to the membrane by a glycosylphosphatidylinositol anchor to induce T cell activation has suggested that these proteins interact with cellular ligands or counter-receptors. The murine Ly-6 antigens and the closely-related human CD59 antigen play important roles in T cell activation, B cell activation and cell-cell interactions. Receptor globulins containing the external domains of Ly-6A.2 or CD59 coupled to hinge-CH2-CH3 regions of human IgG1 were generated. When these fusion proteins were used to stain lymphoid tissues, they selectively reacted with B lymphocytes. On mouse B cells, their target antigen is membrane IgM, while their target on human B cells has not been fully defined. These results suggest that Ly-6/CD59-IgM may be a receptor-counter-receptor pair and may play a role in T-B cell interactions.
Shevach
E M
EM
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.
Korty
P E
PE
D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D000950:Antigens, Ly / Q000276:immunology*; D018801:CD2 Antigens; D018957:CD59 Antigens; D017261:Glycosylphosphatidylinositols / Q000502:physiology*; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D011971:Receptors, Immunologic / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D000950:Antigens, Ly; D018801:CD2 Antigens; D018957:CD59 Antigens; D017261:Glycosylphosphatidylinositols; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins