Increase in bcl-2 oncoprotein and the tolerance to ischemia-induced neuronal death in the gerbil hippocampus.
We studied the expression of bcl-2 oncoprotein in the gerbil hippocampus after transient ischemia. Immunostaining using monoclonal antibody raised against bcl-2 oncoprotein revealed intense immunoreactivity in the CA1 area following 2 min of ischemia, which induced tolerance to subsequent ischemia and prevented delayed neuronal death (DND). Following ischemia for 5 min, however, bcl-2 oncoprotein immunoreactivity was decreased, reflecting neuronal death in the CA1 area. However, pretreatment with ischemia of 2 min that prevented DND due to subsequent ischemia for 5 min, showed increased immunoreactivity. On the other hand, following 1 min of ischemia which failed to induce tolerance, no increase in the bcl-2 oncoprotein was observed. The results evidenced that expression of bcl-2 oncoprotein in the CA1 area following brief ischemia is closely related to the acquisition of resistance to DND.
Shimazaki
K
K
Department of Physiology, Jichi Medical School, Tochigi-ken, Japan.
Ishida
A
A
Kawai
N
N
D000818:Animals; D000911:Antibodies, Monoclonal; D016923:Cell Death; D005849:Gerbillinae; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology*; D002546:Ischemic Attack, Transient / Q000473:pathology*; D009474:Neurons / Q000473:pathology*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2; D013194:Staining and Labeling; D013997:Time Factors
D000911:Antibodies, Monoclonal; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2