title: GDNF: existence of a second transcript in the brain.
abstract: The detection of the glial cell-line derived neurotrophic factor (GDNF) mRNA by RT-PCR in dissociated cell culture of rat embryonic or post-natal brain allowed the amplification of a doublet. The major band corresponded to the expected size and the minor one to a shorter product. We cloned and sequenced the latter product, and thus identified a mRNA potentially encoding for an isoform of the initially described precursor protein involved in GDNF synthesis.
authors.affiliation: Laboratoire de Physiologie, Inserm U318, C.H.U. de Grenoble, France.
authors.forename: N
authors.initials: N
authors.lastname: Cristina
authors.forename: C
authors.initials: C
authors.lastname: Chatellard-Causse
authors.forename: M
authors.initials: M
authors.lastname: Manier
authors.forename: C
authors.initials: C
authors.lastname: Feuerstein
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D051100:Glial Cell Line-Derived Neurotrophic Factor; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D017421:Sequence Analysis; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger