title: Expression of pokeweed lectin binding in murine intestinal Paneth cells.
abstract: Pokeweed (Phytolacca americana) lectin was found to stain the secretory granules in epithelial Paneth cells of small intestine in mice and rats. The distribution of Paneth cells stained with this lectin was identical to that obtained by another immunohistochemical marker for lysozyme. However, in comparison with other immunohistochemical markers, Pokeweed lectin is a more robust method for identifying Paneth cells in histological sections and for studying their secretory granules. Co-expression of the Pokeweed lectin binding sites in some oligomucous cells within the crypts suggested a close developmental link between these two cell types. Only one other non-epithelial cell type was stained by this lectin, and these were migratory lymphocytes found within the villus epithelium and lamina propria. Approximately 20% of these lymphocyte cells were also positive for the expression of CD3+. Pokeweed lectin was therefore used to study changes in the frequency of Paneth cells and intra-epithelial lymphocytes in normal and immunologically compromised animals (following infection with a parasite worm Trichuris muris and in a model of graft-versus-host rejection). This study confirmed that the population of Paneth cells turns over slowly even during conditions of inflammation.
authors.affiliation: CRC Department of Epithelial Biology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
authors.forename: G S
authors.initials: GS
authors.lastname: Evans
authors.forename: S
authors.initials: S
authors.lastname: Chwalinski
authors.forename: G
authors.initials: G
authors.lastname: Owen
authors.forename: C
authors.initials: C
authors.lastname: Booth
authors.forename: A
authors.initials: A
authors.lastname: Singh
authors.forename: C S
authors.initials: CS
authors.lastname: Potten
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002454:Cell Differentiation; D003594:Cytoplasmic Granules / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology / Q000473:pathology; D006651:Histocytochemistry; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry / Q000166:cytology* / Q000473:pathology; D007421:Intestine, Small / Q000737:chemistry / Q000166:cytology* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011043:Pokeweed Mitogens / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling / Q000379:methods; D014257:Trichuriasis / Q000276:immunology / Q000473:pathology
chemical_list: D011043:Pokeweed Mitogens