Pristane retards clearance of particulate materials from the peritoneal cavity of laboratory mice.
The effect of intraperitoneal pristane on the movement of India ink particles and water-based radio-opaque dye injected into the peritoneal cavity of mice was examined. There was a marked difference between pristane-treated mice and unmanipulated controls in terms of particle retention; unmanipulated mice cleared India ink particles via lymphatic drainage within 24 h following injection, whereas pristane-treated mice retained particles for as long as 22 days post-injection. In contrast, all mice displayed similar kinetics of removal of radio-opaque dye. We conclude that the movement of particulate matter from the peritoneal cavity is retarded in pristane-treated mice whereas removal of water-based materials is unaffected. The implications for generation of ascites using hybridoma cells in pristane-primed mice is discussed.
Moore
J M
JM
Department of Pathology, University of Connecticut Health Center, Farmington 06032.
Rajan
T V
TV
D000818:Animals; D001201:Ascites / Q000139:chemically induced*; D002244:Carbon; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D004396:Coloring Agents / Q000378:metabolism; D003973:Diatrizoate / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008208:Lymphatic System / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity; D013194:Staining and Labeling; D013729:Terpenes / Q000008:administration & dosage / Q000633:toxicity*
D002273:Carcinogens; D004396:Coloring Agents; D013729:Terpenes; C028433:chinese ink; D003973:Diatrizoate; C009042:pristane; D002244:Carbon