title: Human cysteine dioxygenase type I: primary structure derived from base sequencing of cDNA.
abstract: The base sequence of cDNA encoding the complete human liver cysteine dioxygenase type I (CDO-I; EC 1.13.11.20) message, and the derived amino-acid sequence are reported. CDO-I is encoded on a single mRNA species (approx. 1.5 kb). Human CDO-I clones were identified by screening a liver cDNA library, and inserts were isolated and sequenced. In addition, human liver total RNA was reverse-transcribed and CDO-I cDNA amplified by PCR using a modified poly-T primer and specific CDO-I primers to give a second source of sequencing template. The CDO-I message encodes a 200 amino-acid residue protein, the sequence of which has greater than 90% homology with the equivalent rat enzyme. The message was not expressed in a human hepatoma cell line (Hep G2).
authors.affiliation: Department of Clinical Neurology, University of Birmingham, Queen Elizabeth Hospital, UK.
authors.forename: K P
authors.initials: KP
authors.lastname: McCann
authors.forename: M T
authors.initials: MT
authors.lastname: Akbari
authors.forename: A C
authors.initials: AC
authors.lastname: Williams
authors.forename: D B
authors.initials: DB
authors.lastname: Ramsden
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D050558:Cysteine Dioxygenase; D015342:DNA Probes; D018076:DNA, Complementary; D049308:Dioxygenases; D015723:Gene Library; D006801:Humans; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
chemical_list: D015342:DNA Probes; D018076:DNA, Complementary; D012333:RNA, Messenger; D010105:Oxygenases; D049308:Dioxygenases; D050558:Cysteine Dioxygenase; D012194:RNA-Directed DNA Polymerase