title: [Proliferation of pulmonary fibroblasts obtained from rats with bleomycin-induced pulmonary fibrosis and effects of rat rIL-1 alpha on this proliferation].
abstract: To clarify the mechanism of pulmonary fibrosis, the growth rate of fibroblasts obtained from bleomycin (BLM)-treated rats was compared with that of fibroblasts obtained from control rats. Proliferation of fibroblasts obtained from rats at 4 days after BLM instillation (BRF) was significantly more rapid than that of fibroblasts obtained from rats at 4 days after saline instillation (CRF), as assessed by cell counting and 3H-TdR incorporation. CRF and BRF were separated by the method of discontinuous Percoll gradients. Three fibroblast fractions of specific gravity (S.G.), S.G. < 1.036, 1.036 < or = S.G. < 1.050, 1.050 < or = S.G. < 1.062, were obtained. Percentages of the densest fibroblast fraction were 46.2 +/- 5.2 in BRF and 6.4 +/- 2.8 in CRF. The densest fibroblasts in BRF proliferated more rapidly than the least dense fibroblasts. Rat rIL-1 alpha suppressed the proliferation of CRF and BRF, dose dependently. These results suggest that an increase in the densest fibroblasts in the lung might correlate with BLM-induced pulmonary fibrosis and that IL-1 alpha suppresses the proliferation of pulmonary fibroblasts.
authors.affiliation: Third Department of Internal Medicine, University of Tokushima, School of Medicine.
authors.forename: K
authors.initials: K
authors.lastname: Asada
authors.forename: F
authors.initials: F
authors.lastname: Ogushi
authors.forename: H
authors.initials: H
authors.lastname: Takehara
authors.forename: T
authors.initials: T
authors.lastname: Endo
authors.forename: K
authors.initials: K
authors.lastname: Tani
authors.forename: S
authors.initials: S
authors.lastname: Miki
authors.forename: M
authors.initials: M
authors.lastname: Masuda
authors.forename: T
authors.initials: T
authors.lastname: Ogura
mesh_terms: D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D002455:Cell Division; D005347:Fibroblasts / Q000473:pathology; D007370:Interferon Type I / Q000494:pharmacology*; D008168:Lung / Q000473:pathology*; D008297:Male; D011658:Pulmonary Fibrosis / Q000139:chemically induced / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins
chemical_list: D007370:Interferon Type I; D011994:Recombinant Proteins; D001761:Bleomycin