title: A human telomeric protein.
abstract: Telomeres are multifunctional elements that shield chromosome ends from degradation and end-to-end fusions, prevent activation of DNA damage checkpoints, and modulate the maintenance of telomeric DNA by telomerase. A major protein component of human telomeres has been identified and cloned. This factor, TRF, contains one Myb-type DNA-binding repeat and an amino-terminal acidic domain. Immunofluorescent labeling shows that TRF specifically colocalizes with telomeric DNA in human interphase cells and is located at chromosome ends during metaphase. The presence of TRF along the telomeric TTAGGG repeat array demonstrates that human telomeres form a specialized nucleoprotein complex.
authors.affiliation: Laboratory for Cell Biology and Genetics, Rockefeller University, New York, NY 10021, USA.
authors.forename: L
authors.initials: L
authors.lastname: Chong
authors.forename: B
authors.initials: B
authors.lastname: van Steensel
authors.forename: D
authors.initials: D
authors.lastname: Broccoli
authors.forename: H
authors.initials: H
authors.lastname: Erdjument-Bromage
authors.forename: J
authors.initials: J
authors.lastname: Hanish
authors.forename: P
authors.initials: P
authors.lastname: Tempst
authors.forename: T
authors.initials: T
authors.lastname: de Lange
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002467:Cell Nucleus / Q000737:chemistry; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D006367:HeLa Cells; D006801:Humans; D007399:Interphase; D008677:Metaphase; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D016615:Telomere / Q000737:chemistry*; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins