Cloning and analysis of a locus (mcr) involved in mitomycin C resistance in Streptomyces lavendulae.
Two genes (mcrA and mcrB) from Streptomyces lavendulae that together confer resistance to mitomycin C were identified. This DNA appears to comprise a polycistronic operon with a drug-inducible leaderless mRNA. The deduced amino acid sequence of mcrA shows similarity to sequences of a special class of bacterial, plant, and animal oxygen oxidoreductases.
August
P R
PR
Department of Microbiology, University of Minnesota, St. Paul 55108.
Flickinger
M C
MC
Sherman
D H
DH
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D050544:L-Gulonolactone Oxidase; D016685:Mitomycin / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009876:Operon; D010088:Oxidoreductases; D010089:Oxidoreductases, N-Demethylating / Q000737:chemistry; D012329:RNA, Bacterial / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics*; D013401:Sugar Alcohol Dehydrogenases / Q000737:chemistry; D015534:Trans-Activators
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D015534:Trans-Activators; C088146:mcrB protein, Streptomyces lavendulae; D016685:Mitomycin; D010088:Oxidoreductases; D013401:Sugar Alcohol Dehydrogenases; D050544:L-Gulonolactone Oxidase; C071690:reticuline oxidase; D010089:Oxidoreductases, N-Demethylating; C088145:mcrA protein, Streptomyces lavendulae