No requirement of reactive oxygen intermediates in Fas-mediated apoptosis.
Fas is a cell surface molecule that mediates apoptosis, but the intracellular mechanisms leading to apoptosis are not well understood. It is known that diethylmaleate (DEM)-induced cell death can be blocked by substances with antioxidant activity. Here we have studied whether antioxidants have any effect on Fas-mediated apoptosis and show that they are not able to block Fas-mediated apoptosis. Therefore, it seems that reactive oxygen intermediate (ROI)-dependent and -independent mechanisms which lead to apoptosis do exist. Fas-mediated apoptosis probably proceeds via a ROI-independent pathway.
Hug
H
H
Osaka Bioscience Institute, Japan.
Enari
M
M
Nagata
S
S
D000818:Animals; D000906:Antibodies; D000954:Antigens, Surface / Q000276:immunology / Q000502:physiology*; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D008298:Maleates / Q000494:pharmacology; D051379:Mice; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D019014:fas Receptor
D000906:Antibodies; D000954:Antigens, Surface; D000975:Antioxidants; D008298:Maleates; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor; C014476:diethyl maleate