title: The effect of exercise on basophil histamine release in patients with bronchial asthma.
abstract: There is increasing evidence for the role of basophils in allergic bronchial asthma. We studied the potential role of basophils in the pathogenesis of post-exercise-induced bronchoconstriction by measuring the histamine release from basophils both spontaneously and following ConA, FMLP, anti-IgE and TPA treatment. Two groups of patients with allergic asthma were studied: group I consisted of 8 patients with an exercise-induced fall in FEV1 of 20% or more, and group II had 7 patients with bronchial asthma who had less than a 5% fall in FEV1 following exercise. The mean spontaneous histamine release (SHR%) from basophils for group I was significantly larger than that of group II both before as well as at 5-10 and 60 min following exercise. The SHR% at baseline was 25 +/- 10 in group I (mean +/- SD) and 15 +/- 5 in group II (mean +/- SD). At 5-10 min following exercise it was 24 +/- 6 in group I and 11 +/- 2 in group II, while at 60 min following exercise it was 24 +/- 6 in group I and 17 +/- 2 in group II. There was no significant difference between the two groups in the effect of ConA, FMLP, anti-IgE or TPA treatment on basophil histamine release. The enhanced bronchoconstriction by exercise did not affect histamine release either spontaneously or following those 4 stimuli. It was concluded that, although patients with exercise-induced asthma have a greater degree of spontaneous histamine release, this is not affected by induced bronchoconstriction, a finding which does not support a role for basophils in exercise-induced asthma.
authors.affiliation: Institute of Pulmonary and Allergic Diseases, Tel-Aviv-Sourasky Medical Center, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Kivity
authors.forename: I
authors.initials: I
authors.lastname: Schwarz
authors.forename: O
authors.initials: O
authors.lastname: Agami
authors.forename: M
authors.initials: M
authors.lastname: Topilsky
authors.forename: E
authors.initials: E
authors.lastname: Fireman
mesh_terms: D000293:Adolescent; D000328:Adult; D001249:Asthma / Q000276:immunology* / Q000503:physiopathology; D001491:Basophils / Q000276:immunology*; D015444:Exercise / Q000502:physiology*; D005260:Female; D005541:Forced Expiratory Volume; D006632:Histamine / Q000032:analysis; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis; D008297:Male
chemical_list: D007073:Immunoglobulin E; D006632:Histamine