Comparative effects of a highly specific protein kinase C inhibitor, calphostin C and calmodulin inhibitors on angiotensin-stimulated aldosterone secretion.
We have examined the relative roles of the calcium-calmodulin system and protein kinase C in angiotensin-mediated aldosterone secretion. We used a highly specific protein kinase C inhibitor, calphostin C and two well-accepted calmodulin inhibitors, W-7 and calmidazolium. Although both types of inhibitors affected angiotensin-induced aldosterone secretion, as judged by the inhibitory doses of these compounds, angiotensin-evoked aldosterone secretion was more sensitive to calmodulin inhibition than protein kinase C inhibition. Manipulation of OFFracellular calcium by dantrolene and thapsigargin also modified aldosterone secretion significantly.
Ganguly
A
A
Department of Medicine, University of South Florida College of Medicine, Tampa.
Waldron
C
C
D000450:Aldosterone / Q000378:metabolism*; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000502:physiology; D002147:Calmodulin / Q000037:antagonists & inhibitors*; D002417:Cattle; D009281:Naphthalenes; D011083:Polycyclic Compounds / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D012636:Secretory Rate / Q000187:drug effects; D013449:Sulfonamides / Q000494:pharmacology; D015384:Zona Glomerulosa / Q000201:enzymology
D002147:Calmodulin; D009281:Naphthalenes; D011083:Polycyclic Compounds; D013449:Sulfonamides; D000804:Angiotensin II; D000450:Aldosterone; C017967:W 7; D011493:Protein Kinase C; C058819:calphostin C; D002118:Calcium