title: Stimulation of protein kinase C activity by compactin in vascular smooth muscle cells.
abstract: The effect of compactin (a lovastatin analogue) on vascular smooth muscle cells was studied at the level of cell proliferation and protein kinase C. It was observed: a) an inhibition of cell proliferation by compactin at a micromolar range, which was prevented by simultaneous addition of mevalonate; b) a stimulation of DNA synthesis with a shift in the cell cycle kinetics, either in the presence or absence of fetal calf serum and c) an increase in protein kinase C activity in compactin-treated cells in the G1 phase of the cycle. This increase was similar to the one elicited by calyculin A, an inhibitor of protein phosphatases of type PP-1 and PP-2A. It is suggested that compactin behaves as a PP-1/PP-2A protein phosphatase inhibitor, inhibiting proliferation of smooth muscle cells by a block of the cell cycle after the S-phase.
authors.affiliation: Institut für Biochemie und Molekularbiologie, Universität Bern, Switzerland.
authors.forename: N
authors.initials: N
authors.lastname: Latruffe
authors.forename: D
authors.initials: D
authors.lastname: Boscoboinik
authors.forename: A
authors.initials: A
authors.lastname: Azzi
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000960:Hypolipidemic Agents / Q000494:pharmacology; D008148:Lovastatin / Q000031:analogs & derivatives* / Q000494:pharmacology; D008387:Marine Toxins; D008798:Mevalonic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000201:enzymology*; D010080:Oxazoles / Q000494:pharmacology; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats
chemical_list: D004791:Enzyme Inhibitors; D000960:Hypolipidemic Agents; D008387:Marine Toxins; D010080:Oxazoles; C012258:mevastatin; C015367:mevalonolactone; C059041:calyculin A; D008148:Lovastatin; D011493:Protein Kinase C; D010749:Phosphoprotein Phosphatases; D008798:Mevalonic Acid