(E)4-[4-N,N-dimethylaminophenyl]but-3-en-2-one (DMAP) treatment inhibits the radiation-induced micronucleus formation in bone marrow of BALB/c mice.
The frequency of micronucleated polychromatic (MPCE) and normochromatic erythrocytes (MNCE) and polychromatic/normochromatic erythrocyte ratio (P/N ratio) was studied in the bone marrow of BALB/c mice administered with 20 mg/kg body weight of (E)4-[4-N,N-dimethylaminophenyl]but-3-en-2-one (DMAP), a phenylbutenone derivative, 30 min before exposure to different doses of 60Co gamma-radiation. A dose-dependent increase in the frequency of MPCE and MNCE was observed in double-distilled water (DDW) or oil + irradiated and DMAP + irradiated groups. The frequency of MPCE and MNCE was significantly less in the DMAP + irradiated group when compared to the DDW or oil + irradiated groups at all the irradiation doses studied. The P/N ratio declined with increasing radiation dose in DDW or oil + irradiated and DMAP + irradiated groups. The inhibitory effect of irradiation on the P/N ratio was less in the DMAP + irradiated group as compared to the DDW or oil + irradiated groups, as evidenced by the higher P/N ratio in the former than the latter. The P/N ratio was significantly higher in the DMAP + irradiated group from 1 Gy irradiation onwards. The dose-response relationship was linear-quadratic for MPCE, MNCE and P/N ratio for all the groups studied.
Jagetia
G C
GC
Department of Radiobiology, Kasturba Medical College, Manipal, India.
Jacob
P S
PS
Rao
M N
MN
D000814:Aniline Compounds / Q000494:pharmacology*; D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects* / Q000648:ultrastructure; D002074:Butanones / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D016166:Free Radical Scavengers; D005978:Glutathione / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects / Q000528:radiation effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology*
D000814:Aniline Compounds; D002074:Butanones; D016166:Free Radical Scavengers; D011837:Radiation-Protective Agents; C086624:4-(4-N,N-dimethylaminophenyl)but-3-en-2-one; D005978:Glutathione