title: Characterization of the substance P (NK-1) receptor in tunicamycin-treated transfected cells using a photoaffinity analogue of substance P.
abstract: Chinese hamster ovary cells expressing the N-glycosylated substance P (NK-1) receptor were treated with the glycosylation inhibitor tunicamycin and photolabeled with 125I-Bolton-Hunter-p-benzoyl-L-phenylalanine8-substance P. Two radioactive proteins of M(r) 80,000 and 46,000, representing the glycosylated and nonglycosylated substance P (NK-1) receptor, respectively, were observed. The IC50 for the inhibition of photolabeling of both receptor forms was 0.3 +/- 0.1 nM for substance P and 30 +/- 5 nM for neurokinin A (substance K). Thus, glycosylation of the substance P (NK-1) receptor has no detectable effect on the affinity of the substance P (NK-1) receptor for substance P or neurokinin A (substance K).
authors.affiliation: Department of Pharmacology and Experimental Therapeutics, Boston University School of Medicine, MA 02118.
authors.forename: R
authors.initials: R
authors.lastname: Kage
authors.forename: A D
authors.initials: AD
authors.lastname: Hershey
authors.forename: J E
authors.initials: JE
authors.lastname: Krause
authors.forename: N D
authors.initials: ND
authors.lastname: Boyd
authors.forename: S E
authors.initials: SE
authors.lastname: Leeman
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D006031:Glycosylation; D008027:Light; D015288:Neurokinin A; D009844:Oligosaccharides / Q000032:analysis; D018040:Receptors, Neurokinin-1 / Q000032:analysis* / Q000378:metabolism*; D013373:Substance P / Q000031:analogs & derivatives* / Q000378:metabolism*; D014162:Transfection; D014415:Tunicamycin / Q000494:pharmacology*
chemical_list: D009844:Oligosaccharides; D018040:Receptors, Neurokinin-1; D014415:Tunicamycin; D013373:Substance P; D015288:Neurokinin A