Neutrophil activation after percutaneous transluminal coronary angioplasty.
We investigated whether percutaneous transluminal coronary angioplasty (PTCA) would induce neutrophil activation in patients with coronary artery disease. Blood samples were taken from the coronary sinus in 14 patients who underwent PTCA and in 9 control subjects who underwent coronary arteriography (CAG). Flow cytometry was used to measure membrane surface expression of beta 2 integrin (CD11b) and the generation of hydrogen peroxide in neutrophils after ex vivo phorbol myristate acetate stimulation by 2,'7'-dichlorofluorescein. Neutrophil elastase was measured by an immunoenzymatic method. Surface expression of CD11b increased significantly, approximately twofold, after PTCA but not after CAG. Mean fluorescence intensity of 2',7'-dichlorofluorescein in stimulated neutrophils decreased significantly after PTCA, suggesting a previous in vivo activation, but not after CAG. Neutrophil elastase increased significantly after PTCA but not after CAG. These data indicate that PTCA induces neutrophil activation and suggest that neutrophils may contribute to the ischemic injury.
Ikeda
H
H
Third Department of Internal Medicine, Kurume University School of Medicine, Japan.
Nakayama
H
H
Oda
T
T
Kuwano
K
K
Yamaga
A
A
Ueno
T
T
Yoh
M
M
Hiyamuta
K
K
Koga
Y
Y
Toshima
H
H
D000328:Adult; D000368:Aged; D015906:Angioplasty, Balloon, Coronary / Q000009:adverse effects; D018821:CD18 Antigens; D002462:Cell Membrane / Q000378:metabolism; D017023:Coronary Angiography; D003327:Coronary Disease / Q000000981:diagnostic imaging / Q000276:immunology / Q000628:therapy*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D016023:Integrins / Q000032:analysis; D019272:Leukocyte Elastase; D016177:Macrophage-1 Antigen / Q000032:analysis; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000209:etiology / Q000276:immunology*; D018375:Neutrophil Activation / Q000502:physiology*; D009504:Neutrophils / Q000378:metabolism; D010196:Pancreatic Elastase / Q000097:blood
D018821:CD18 Antigens; D016023:Integrins; D016177:Macrophage-1 Antigen; D006861:Hydrogen Peroxide; D010196:Pancreatic Elastase; D019272:Leukocyte Elastase