title: Univariate tolerance regions for fibrinogen, antithrombin III, protein C, protein S, plasminogen and alpha 2-antiplasmin in children using the new Automated Coagulation Laboratory (ACL) method.
abstract: To avoid misclassification of lowered or enhanced coagulation proteins in childhood the purpose of this study was to establish functional normal ranges for healthy children aged 6 months to 16 years. PT, aPTT, fibrinogen, antithrombin III, protein C, protein S, plasminogen and alpha 2-antiplasmin were tested using the new Automated Coagulation Laboratory (ACL) method. Values for PT, aPTT, fibrinogen, antithrombin III, plasminogen and alpha 2-antiplasmin were closely comparable in all children although we found a minimum range of 61% in children aged 8 to 16 years in plasma concentrations of alpha 2-antiplasmin. Children < 2.5 years showed reduced lower boundaries encompassing 95% of the population for protein C and protein S activity, although medians for protein S activity were similar in all children. The rapid and automatic determination of functional coagulation proteins using chromogenic substrates on the ACL 300 (25-50 microliters citrated plasma/test) renders the possibility to realize a screening program for inherited thrombotic syndromes in a routine laboratory.
authors.affiliation: Dept. Haematology and Oncology, University Hospital Frankfurt/Main.
authors.forename: U
authors.initials: U
authors.lastname: Nowak-Göttl
authors.forename: M
authors.initials: M
authors.lastname: Funk
authors.forename: G
authors.initials: G
authors.lastname: Mosch
authors.forename: B
authors.initials: B
authors.lastname: Wegerich
authors.forename: B
authors.initials: B
authors.lastname: Kornhuber
authors.forename: H K
authors.initials: HK
authors.lastname: Breddin
mesh_terms: D000293:Adolescent; D000328:Adult; D000990:Antithrombin III / Q000032:analysis; D001331:Automation; D001777:Blood Coagulation / Q000502:physiology*; D001780:Blood Coagulation Tests / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D006801:Humans; D007223:Infant; D008297:Male; D010958:Plasminogen / Q000032:analysis; D011486:Protein C / Q000032:analysis; D017293:Protein S / Q000032:analysis; D012016:Reference Values; D000979:alpha-2-Antiplasmin / Q000032:analysis
chemical_list: D011486:Protein C; D017293:Protein S; D000979:alpha-2-Antiplasmin; D000990:Antithrombin III; D005340:Fibrinogen; D010958:Plasminogen