title: Nitric oxide synthase increases in hypothalamic magnocellular neurons after salt loading in the rat. An immunohistochemical and in situ hybridization study.
abstract: Magnocellular hypothalamic neurons of the paraventricular (PVN) and supraoptic (SON) nuclei have been shown to contain a wide variety of messenger molecules in addition to vasopressin and oxytocin, including the nitric oxide (NO)-synthesizing enzyme (NOS). In this paper we have investigated the effects of salt loading on the expression of NOS by means of immunohistochemistry and in-situ hybridization. The results show an increase in the number of NOS-immunoreactive (IR) neurons both in the PVN and the SON after 5 and 14 days of salt loading. Several of these neurons were double labelled with vasopressin antiserum. In situ hybridization showed a marked increase in the number of neurons expressing NOS mRNA and a stronger signal in individual neurons. The present results suggest a role for NO in the magnocellular hypothalamic system after salt loading.
authors.affiliation: Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
authors.forename: M J
authors.initials: MJ
authors.lastname: Villar
authors.forename: S
authors.initials: S
authors.lastname: Ceccatelli
authors.forename: M
authors.initials: M
authors.lastname: Rönnqvist
authors.forename: T
authors.initials: T
authors.lastname: Hökfelt
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis*; D000818:Animals; D001345:Autoradiography; D007031:Hypothalamus / Q000166:cytology / Q000201:enzymology*; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009474:Neurons / Q000201:enzymology*; D019001:Nitric Oxide Synthase; D010121:Oxytocin / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012462:Saline Solution, Hypertonic; D014667:Vasopressins / Q000276:immunology / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012462:Saline Solution, Hypertonic; D014667:Vasopressins; D010121:Oxytocin; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases