Analysis of the mouse and rat CFTR promoter regions.
To gain insights into the regulation of the mouse and rat cystic fibrosis transmembrane conductance regulator (CFTR) genes, we cloned and sequenced their respective upstream promoter regions. DNA sequence analysis from either side of exon 1 revealed a complete divergence from the human DNA sequence except for three DNA motifs which consist of a 34 bp stretch and two intron specific elements. Highlights of both the rat and mouse promoter sequences include an extensive purine rich stretch, a Y-box motif and putative Sp1, AP1 sites. Transfection of mouse promoter deletional constructs into expressing and non-expressing CFTR murine cell lines revealed that the Pu.Py stretch and the Y-box act, respectively, as negative and positive elements of basal transcription that confer no apparent tissue specificity.
Denamur
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Department of Laboratory Medicine, University of California, San Francisco 94143-0134.
Chehab
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D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D016384:Consensus Sequence; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005091:Exons / Q000235:genetics; D005260:Female; D005796:Genes; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice / Q000235:genetics*; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats / Q000235:genetics*; D013045:Species Specificity
C505032:CFTR protein, human; D008565:Membrane Proteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator