Importance of the fiberoptic endoscope cleaning procedure for detection of Helicobacter pylori in gastric biopsy specimens by PCR.
A 16S ribosomal DNA-based PCR appeared to be a sensitive test for the detection of infection by Helicobacter pylori in 31 patients when compared with culturing and histological and serological techniques. For five patients, PCR was the only test with a positive result. H. pylori DNA was also found in gastrointestinal equipment even after standard intensive combined manual and machine cleaning. We therefore conclude that a reliable validation of PCR for the detection of H. pylori in gastric biopsy specimens is possible only when the cleaning and disinfection method used has been proven to remove all H. pylori DNA from gastrointestinal equipment. An adequate cleaning and disinfection method for the removal of H. pylori DNA from fiberoptic endoscopes is described.
Roosendaal
R
R
Department of Clinical Microbiology, Free University Hospital, Amsterdam, The Netherlands.
Kuipers
E J
EJ
van den Brule
A J
AJ
Peña
A S
AS
Uyterlinde
A M
AM
Walboomers
J M
JM
Meuwissen
S G
SG
de Graaff
J
J
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004203:Disinfection / Q000379:methods*; D005189:False Positive Reactions; D005336:Fiber Optic Technology / Q000295:instrumentation; D005756:Gastritis / Q000175:diagnosis / Q000382:microbiology; D020699:Gastroscopes; D016481:Helicobacter Infections / Q000175:diagnosis / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013270:Stomach / Q000382:microbiology
D017931:DNA Primers; D015342:DNA Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S