title: [A new liver support system composed of functional human cells and a radial-flow bioreactor].
abstract: An artificial liver will be useful for the treatment of acute hepatic failure and a bridge of liver transplantation. The current reports suggest that the hybrid type of artificial liver composed of functional human liver cells and a bioreactor is practical for clinical use. In the present study, we succeeded high density culture on a large-scale of human functional hepatoma (JHH-7) using a newly developed radial flow packed-bed bioreactor. Since the shear stress of this bioreactor is lower than the other type, high density culture without cell damage is possible. JHH-7 cells produced large amounts of human albumin and other liver specific proteins, and then have the function of ammonia metabolism in the system. This study suggests that a radial flow bioreactor will be developed as a new type of artificial liver.
authors.affiliation: Department of Internal Medicine (I), Jikei University School of Medicine, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kawada
authors.forename: S
authors.initials: S
authors.lastname: Nagamori
authors.forename: H
authors.initials: H
authors.lastname: Aizaki
authors.forename: K
authors.initials: K
authors.lastname: Fukaya
authors.forename: M
authors.initials: M
authors.lastname: Niiya
authors.forename: T
authors.initials: T
authors.lastname: Matsuura
authors.forename: H
authors.initials: H
authors.lastname: Sujino
authors.forename: S
authors.initials: S
authors.lastname: Hasumura
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
mesh_terms: D000418:Albumins / Q000096:biosynthesis; D000641:Ammonia / Q000378:metabolism; D001187:Artificial Organs; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008113:Liver Neoplasms / Q000473:pathology; D010101:Oxygen Consumption; D014407:Tumor Cells, Cultured; D014508:Urea / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000096:biosynthesis
chemical_list: D000418:Albumins; D000509:alpha-Fetoproteins; D000641:Ammonia; D014508:Urea; D005947:Glucose