title: Immunohistochemistry with keratin, vimentin, desmin, and alpha-smooth muscle actin monoclonal antibodies in canine mammary gland: normal mammary tissue.
abstract: Normal canine mammary gland tissue was studied immunohistochemically with monoclonal antibodies (MoAbs) directed against various human keratin types, vimentin, desmin, and alpha-smooth muscle actin. Both ductal and alveolar luminal cells were immunoreactive with MoAbs recognizing respectively human keratins no. 7, 8, 18 and 19. In addition, some ductal luminal cells were labelled with a keratin 4 and a keratin 10 MoAb. Basal/myoepithelial cells were immunoreactive only with MoAbs directed against keratin 14, keratins 14 and 17, and alpha-smooth muscle actin. The vimentin MoAb merely labelled solitary loose intraluminal cells representing macro-phages or sloughed epithelial cells. These findings correspond largely to observations made in human breast tissue.
authors.affiliation: Department of Pathology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
authors.forename: J H
authors.initials: JH
authors.lastname: Vos
authors.forename: T S
authors.initials: TS
authors.lastname: van den Ingh
authors.forename: W
authors.initials: W
authors.lastname: Misdorp
authors.forename: R F
authors.initials: RF
authors.lastname: Molenbeek
authors.forename: F N
authors.initials: FN
authors.lastname: van Mil
authors.forename: G R
authors.initials: GR
authors.lastname: Rutteman
authors.forename: D
authors.initials: D
authors.lastname: Ivanyi
authors.forename: F C
authors.initials: FC
authors.lastname: Ramaekers
mesh_terms: D000199:Actins / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D003893:Desmin / Q000032:analysis / Q000276:immunology; D004285:Dogs / Q000033:anatomy & histology*; D007124:Immunoenzyme Techniques / Q000662:veterinary; D007381:Intermediate Filament Proteins / Q000032:analysis* / Q000276:immunology; D007633:Keratins / Q000032:analysis / Q000276:immunology; D008321:Mammary Glands, Animal / Q000033:anatomy & histology* / Q000737:chemistry; D014746:Vimentin / Q000032:analysis / Q000276:immunology
chemical_list: D000199:Actins; D000911:Antibodies, Monoclonal; D003893:Desmin; D007381:Intermediate Filament Proteins; D014746:Vimentin; D007633:Keratins