title: Effects of lindane exposure on rainbow trout (Oncorhynchus mykiss) immunity. III. Effect on nonspecific immunity and B lymphocyte functions.
abstract: The effect of the organochlorine insecticide lindane (gamma-hexachlorocyclohexane) was examined on some major immune functions of rainbow trout (Oncorhynchus mykiss) on the chemiluminescent response of pronephric cells (PMA-induced) in phagocytosis, on the proliferation of lymphocytes with B and T mitogens, and on the number of B lymphocytes analyzed by cytofluorometry. Two different methods of exposure were tried via food (first protocol) and via a single intraperitoneal injection (second protocol). After the oral contamination at a daily body dose of 1 mg/kg for 30 days, a decreased chemiluminescent response was observed with persisted for two more weeks and disappeared over 1.5 months. No effect was observed on lymphocyte proliferation and on the number of circulating B lymphocytes. In the second protocol lindane was administered intraperitoneally at 10, 50, or 100 mg/kg body wt. After 45 days the lymphocyte proliferation of B cells was depressed but not the T cell one. The B cells number in head kidney as measured by cytofluorometry was not significantly modified. Some nonspecific immunity parameters in sera were significantly modified.
authors.affiliation: Laboratoire de Toxicologie Métabolique et Ecotoxicologie, INRA-ENVL, Marcy l'Etiole, France.
authors.forename: M
authors.initials: M
authors.lastname: Dunier
authors.forename: A K
authors.initials: AK
authors.lastname: Siwicki
authors.forename: J
authors.initials: J
authors.lastname: Scholtens
authors.forename: S
authors.initials: S
authors.lastname: Dal Molin
authors.forename: C
authors.initials: C
authors.lastname: Vergnet
authors.forename: M
authors.initials: M
authors.lastname: Studnicka
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D002570:Ceruloplasmin / Q000276:immunology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004350:Drug Residues / Q000032:analysis; D001556:Hexachlorocyclohexane / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects; D009113:Muramidase / Q000097:blood / Q000276:immunology; D009504:Neutrophils / Q000187:drug effects; D017686:Oncorhynchus mykiss / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects
chemical_list: D001556:Hexachlorocyclohexane; D002570:Ceruloplasmin; D009113:Muramidase