T cell subset distribution and B cell hyperreactivity in mice expressing interleukin-4 under the control of major histocompatibility complex class I regulatory sequences.
Transgenic mice in which interleukin-4 (IL-4) is expressed under the control of the major histocompatibility complex (MHC) class I regulatory sequences show low level expression of IL-4 in all organs investigated. Several weeks after birth the animals develop thymus hypoplasia with a loss of CD4+CD8+ double-positive cells and a relative increase in the mature population, especially, and in contrast to previously published lines, the CD4+ single-positive cells. In the periphery, T lymphocytes eventually decline, CD8+ cells being more strongly affected. Many of the residual T cells exhibit the CD44highMel-14low phenotype of antigenically experienced T cells. B cells also show an activated phenotype with respect to size, MHC class II and CD23 expression, are more readily stimulated by anti-mu F(ab')2 antibodies than are B cells from control littermates, and show a higher spontaneous and antigen-induced production of IgG1 and IgE.
Erb
K J
KJ
Institut für Virologie and Immunbiologie, Universität Würzburg, Germany.
Holtschke
T
T
Muth
K
K
Horak
I
I
Schimpl
A
A
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000502:physiology*; D002352:Carrier Proteins / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis; D002478:Cells, Cultured; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D018960:Hyaluronan Receptors; D016130:Immunophenotyping; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000235:genetics*; D019041:L-Selectin; D051379:Mice; D008822:Mice, Transgenic; D011956:Receptors, Cell Surface / Q000032:analysis; D016201:Receptors, Lymphocyte Homing / Q000032:analysis; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology; D016176:T-Lymphocyte Subsets / Q000502:physiology*
D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D015395:Histocompatibility Antigens Class I; D018960:Hyaluronan Receptors; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D019041:L-Selectin; D015847:Interleukin-4