title: Increased expression of alpha 4 beta 1 and alpha 5 beta 1 integrins on HTLV-I-infected lymphocytes.
abstract: T cells interact with the extracellular matrix via integrin receptors and these interactions affect both cellular localization and proliferation. The importance of these interactions in retrovirus-induced diseases, however, remains less clear. In the present study, we investigated changes in T cell adhesion to extracellular matrix proteins by HTLV-I expressing cell lines and human peripheral blood lymphocytes infected with HTLV-I by cocultivation. Cell lines and acutely infected primary peripheral blood lymphocytes demonstrated enhanced adhesion to fibronectin. Acute infection of peripheral blood lymphocytes increased the expression of alpha 5 beta 1 and alpha 4 beta 1 integrins. Antibodies to the alpha 4, alpha 5, and beta 1 subunits inhibited attachment of infected cells to fibronectin. We conclude that HTLV-I infection is associated with an increase in the expression of both the classical fibronectin receptor and the receptor for the alternatively spliced domain of fibronectin on peripheral blood lymphocytes. HTLV-I-related alterations in cell surface adhesion molecules may contribute to the abnormal proliferation of T cells in adult T cell leukemia (ATL) or to the abnormal localization of activated or infected T cells to the central nervous system of patients with tropical spastic paraparesis/HTLV-I-associated myelopathy (TSP/HAM).
authors.affiliation: Laboratory of Molecular Virology, Food and Drug Administration, Rockville, Maryland 20852.
authors.forename: S
authors.initials: S
authors.lastname: Dhawan
authors.forename: B S
authors.initials: BS
authors.lastname: Weeks
authors.forename: F
authors.initials: F
authors.lastname: Abbasi
authors.forename: H R
authors.initials: HR
authors.lastname: Gralnick
authors.forename: A L
authors.initials: AL
authors.lastname: Notkins
authors.forename: M E
authors.initials: ME
authors.lastname: Klotman
authors.forename: K M
authors.initials: KM
authors.lastname: Yamada
authors.forename: P E
authors.initials: PE
authors.lastname: Klotman
mesh_terms: D002448:Cell Adhesion; D002461:Cell Line, Transformed; D005353:Fibronectins; D015368:Human T-lymphotropic virus 1 / Q000254:growth & development*; D006801:Humans; D039041:Integrin alpha4beta1; D016023:Integrins / Q000096:biosynthesis*; D007797:Laminin; D008214:Lymphocytes / Q000382:microbiology*; D015493:Paraparesis, Tropical Spastic / Q000209:etiology; D017469:Receptors, Fibronectin; D016029:Receptors, Very Late Antigen / Q000096:biosynthesis*; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis
chemical_list: D005353:Fibronectins; D039041:Integrin alpha4beta1; D016023:Integrins; D007797:Laminin; D017469:Receptors, Fibronectin; D016029:Receptors, Very Late Antigen; D015682:Retroviridae Proteins, Oncogenic; C060954:p24 protein, Human T-lymphotropic virus 1