title: Effects of galanin on amylase secretion from dispersed rat pancreatic acini.
abstract: Dispersed rat pancreatic acini were used to determine the effect of galanin on the exocrine pancreas and on basal and secretagogue-stimulated amylase secretion. Basal amylase secretion and amylase release stimulated by cholecystokinin octapeptide, bombesin, 12-o-tetradecanoyl-phorbol-13-acetate (TPA), secretin, and vasoactive intestinal peptide were not affected by galanin in doses ranging from 10(-12) to 10(-6) M. Galanin, however, significantly inhibited the amylase release stimulated by sub- and supramaximal doses of carbachol. A time course study showed that the inhibition by galanin occurred during the sustained phase of carbachol-stimulated amylase secretion. The inhibitory action of galanin disappeared in acini obtained from animals pretreated with pertussis toxin (PTX). These results suggest that galanin inhibits carbachol-stimulated amylase secretion through a mechanism related to a PTX-sensitive G protein.
authors.affiliation: Third Department of Internal Medicine, Tohoku University School of Medicine, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Kashimura
authors.forename: T
authors.initials: T
authors.lastname: Shimosegawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kikuchi
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Koizumi
authors.forename: T
authors.initials: T
authors.lastname: Mochizuki
authors.forename: N
authors.initials: N
authors.lastname: Yanaihara
authors.forename: T
authors.initials: T
authors.lastname: Toyota
mesh_terms: D000681:Amylases / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001481:Basal Metabolism; D019004:Galanin; D066298:In Vitro Techniques; D008297:Male; D009479:Neuropeptides / Q000494:pharmacology*; D010179:Pancreas / Q000187:drug effects* / Q000201:enzymology; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D009479:Neuropeptides; D010455:Peptides; D019004:Galanin; D000681:Amylases