title: Ultrastructural localization of NADPH-diaphorase and colocalization of nitric oxide synthase in endothelial cells of the rabbit aorta.
abstract: This is the first report on the ultrastructural pattern of distribution of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) in endothelial cells, using the rabbit aorta, and its colocalization with the neuronal isoform (type I) of nitric oxide synthase. About 30% of the endothelial cells showed a positive reaction for NADPH-d compared to about 6% for nitric oxide synthase immunoreactivity. Simultaneous double histochemical-immunocytochemical labelling procedures indicate that all of the cells displaying nitric oxide synthase-positive reactivity also contained NADPH-d; the remainder of NADPH-d-positive endothelial cells were negative for this isoform of nitric oxide synthase. Nitric oxide synthase-immunogold labelling was mostly associated with free ribosomes, while NADPH-d activity was distributed largely in patches in the cytoplasm and in association with the cell membrane.
authors.affiliation: Department of Anatomy and Developmental Biology, University College London, UK.
authors.forename: A
authors.initials: A
authors.lastname: Loesch
authors.forename: A
authors.initials: A
authors.lastname: Belai
authors.forename: G
authors.initials: G
authors.lastname: Burnstock
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D000818:Animals; D001013:Aorta, Thoracic / Q000201:enzymology* / Q000648:ultrastructure; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron / Q000379:methods; D009252:NADPH Dehydrogenase / Q000032:analysis*; D019001:Nitric Oxide Synthase; D011817:Rabbits
chemical_list: D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase