Interleukin-8 production by CD16-CD56bright natural killer cells in the human early pregnancy decidua.
Decidual CD16-CD56bright natural killer (NK) cells were sorted from the decidual mononuclear cells (MNC) at the early pregnancy using a fluorescence activated cell sortor. The CD16-CD56bright NK cell population occupies a major population in the decidual MNC, in contrast to a very small population (< 1%) in the peripheral blood MNC. These decidual CD16-CD56bright NK cells produced a large amount of IL-8, i. e., mean of 96.7 +/- 19.8 ng/ml in the 24 hr-cultured supernatants without any stimulant, which was comparable to the IL-8 production by LPS-stimulated peripheral blood MNC. Most of the IL-8 was ascribable to the production from decidual CD16-CD56bright NK cells. Intracytoplasmic IL-8 in the decidual CD56bright NK cells was also detected by flow cytometry. RT-PCR methods confirmed IL-8 mRNA expression in this population, while no or very scarce expression of IL-1 alpha and IL-1 beta mRNA was observed. The present study is a first observation revealing that decidual CD16-CD56bright NK cells express IL-8 mRNA and produce IL-8.
Saito
S
S
Department of Obstetrics and Gynecology, Nara Medical University, Japan.
Kasahara
T
T
Sakakura
S
S
Enomoto
M
M
Umekage
H
H
Harada
N
N
Morii
T
T
Nishikawa
K
K
Narita
N
N
Ichijo
M
M
D015703:Antigens, CD / Q000032:analysis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D001483:Base Sequence; D019002:CD56 Antigen; D017931:DNA Primers; D003656:Decidua / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy / Q000276:immunology*; D011261:Pregnancy Trimester, First; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D017452:Receptors, IgG / Q000032:analysis*
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D019002:CD56 Antigen; D017931:DNA Primers; D007375:Interleukin-1; D016209:Interleukin-8; D012333:RNA, Messenger; D017452:Receptors, IgG