title: Mechanisms of vancomycin-induced histamine release from rat peritoneal mast cells.
abstract: The mechanisms of vancomycin (VCM)-induced histamine release were studied with rat peritoneal mast cells. VCM (> 1 x 10(-3) M) released histamine from the isolated mast cells in a dose-dependent and noncytotoxic manner. In the absence of extracellular Ca2+, the histamine release was reduced markedly. When the intracellular Ca2+ was depleted, it was further decreased. The Fura-2-loaded single mast cells showed a biphasic increase in the intracellular Ca2+ concentration ([Ca2+]i) by VCM: the first transient and the second sustained components. In the absence of extracellular Ca2+, the transient component was unchanged, while the sustained component was eliminated completely. The IP3 content in the mast cells increased within 10 s after the application of VCM. These results suggest that VCM release histamine from rat peritoneal mast cells via an IP3 production and increase in [Ca2+]i.
authors.affiliation: Second Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Horinouchi
authors.forename: K
authors.initials: K
authors.lastname: Abe
authors.forename: K
authors.initials: K
authors.lastname: Kubo
authors.forename: M
authors.initials: M
authors.lastname: Oka
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D006632:Histamine / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000096:biosynthesis; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D010716:Phosphatidylinositols / Q000378:metabolism; D011112:Polymyxin B / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013050:Spectrometry, Fluorescence; D014640:Vancomycin / Q000494:pharmacology*
chemical_list: D010716:Phosphatidylinositols; D014640:Vancomycin; D006632:Histamine; D015544:Inositol 1,4,5-Trisphosphate; D007770:L-Lactate Dehydrogenase; D011112:Polymyxin B; D002118:Calcium