title: Effect of minoxidil on the mobility and differentiation of cultivated human keratinocytes.
abstract: The mode of topical action of minoxidil (U-10,858, CAS 38304-91-5) ist not entirely clear. The influence of minoxidil on the ultimate differentiation of keratinocytes was investigated. Human keratinocytes (HaCaT-cells) were incubated with minoxidil containing culture medium (10-250 micrograms/ml). Minoxidil inhibited in a concentration dependent manner cell mobility whereas the adhesion area and the cell circumference were increased. The minoxidil treated cultures had a higher desmosome density compared to parallel control cultures and they expressed the suprabasal keratins 1 and 10 (indicating progress in differentiation) earlier and to a larger extent than the controls. Keratins were revealed immunohistochemically and by two-dimensional polyacrylamide gel electrophoresis. The results suggest that minoxidil supports the differentiation of human keratinocytes.
authors.affiliation: Abteilung I, Johann Wolfgang Goethe-Universität, Frankfurt/Main, Fed. Rep. of Germany.
authors.forename: A
authors.initials: A
authors.lastname: Bernd
authors.forename: C
authors.initials: C
authors.lastname: Jackel
authors.forename: K
authors.initials: K
authors.lastname: Dold
authors.forename: F
authors.initials: F
authors.lastname: Görmar
authors.forename: C
authors.initials: C
authors.lastname: Theilig
authors.forename: M
authors.initials: M
authors.lastname: Breuer
authors.forename: A
authors.initials: A
authors.lastname: Ramirez-Bosca
authors.forename: J
authors.initials: J
authors.lastname: Bereiter-Hahn
authors.forename: H
authors.initials: H
authors.lastname: Holzmann
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D003896:Desmosomes / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000187:drug effects* / Q000648:ultrastructure; D007633:Keratins / Q000096:biosynthesis; D008914:Minoxidil / Q000494:pharmacology*
chemical_list: D008914:Minoxidil; D007633:Keratins