Hispidospermidin, a novel phospholipase C inhibitor produced by Chaetosphaeronema hispidulum (Cda) Moesz NR 7127. I. Screening, taxonomy, and fermentation.
A novel phospholipase C inhibitor, hispidospermidin, was discovered from a fungal culture broth. The producing fungus, NR 7127, formed abundant pycnidia on banana leaf agar under near UV light. The ostiolate pycnidia were dark colored with a short beak possessing numerous protruding setae. The conidiogeneous cells were phialidic. The conidia were hyaline, 1 septate, smooth and spindle-shaped. From these distinctive characteristics, this strain was identified as Chaetosphaeronema hispidulum (Cda) Moesz of the Coelomycetes. Hispidospermidin was produced in a 50-liter jar fermentor containing 2% glucose, 2% potato starch, 2% Toast soya, 0.5% yeast extract, 0.25% NaCl, 0.0005% ZnSO4.7H2O, 0.0005% CuSO4.5H2O, 0.0005% MnSO4.4H2O, 0.32% CaCO3, and 0.3% Nissan disfoam CA-115. Fermentation was conducted at 27 degrees C at an aeration rate of 30 liters/minute and agitated at 500 rpm for 95 hours. Maximum production yield of hispidospermidin was observed after 72 hours. Hispidospermidin inhibited rat brain phospholipase C at 16 microM of IC50. This is the first recorded discovery of a secondary metabolite from the genus Chaetosphaeronema.
Yanagisawa
M
M
Nippon Roche Research Center, Kanagawa, Japan.
Sakai
A
A
Adachi
K
K
Sano
T
T
Watanabe
K
K
Tanaka
Y
Y
Okuda
T
T
D000818:Animals; D002597:Chaetomium / Q000145:classification / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D005285:Fermentation; D011083:Polycyclic Compounds / Q000302:isolation & purification* / Q000494:pharmacology*; D051381:Rats; D013095:Spermidine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D010738:Type C Phospholipases / Q000037:antagonists & inhibitors*
D011083:Polycyclic Compounds; C086148:hispidospermidin; D010738:Type C Phospholipases; D013095:Spermidine