Enhanced secretion of endothelin by endothelial cells in response to hemoglobin.
Confluent cultures of bovine aortic endothelium in serum-free medium were exposed to increasing concentrations (10(-6)-10(-4) M) of freshly prepared erythrocyte lysates (primarily hemoglobin). Hemoglobin increased endothelin-1 secretion into the medium in a dose-dependent manner after 24 hours. The enhanced secretion of endothelin-1 in response to hemoglobin was sustained for 72 hours, suggesting active production and secretion of endothelin-1 rather than release from intracellular pools. Secreted endothelin-1 in the medium was characterized by high-performance liquid chromatography coupled with radioimmunoassay. Endothelin-1, a potent and long-lasting vasoconstrictor, may be one of the causative factors of cerebral vasospasm after subarachnoid hemorrhage. Oxyhemoglobin, derived from periarterial clot, may play an important role in the secretion of endothelin-1 in cerebral vasospasm.
Fuwa
I
I
Department of Neurosurgery, University of Washington, Seattle.
Mayberg
M
M
Gadjusek
C
C
Harada
T
T
Luo
Z
Z
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D016232:Endothelins / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D006454:Hemoglobins / Q000502:physiology*; D006801:Humans; D010108:Oxyhemoglobins / Q000502:physiology
D016232:Endothelins; D006454:Hemoglobins; D010108:Oxyhemoglobins