Cross-linking of a sequential epitope within the beta-chain of HLA-DR/DP molecules suppressing B lymphocyte growth and inducing homotypic cell aggregation.
A monoclonal antibody (SU2) directed against HLA class II antigens has been produced by immunizing BALB/c mice with a lymphoblastoid cell line, RPMI-8866 cells. The specificity of this mAb has been determined using a panel of HLA class II transfectants. SU2 stained all HLA-DR and HLA-DP transfectants tested, but reacted with only one HLA-DQ (HLA-DQw2). From comparison of the available data sequences of known HLA class II molecules, it appeared that the epitope recognized by the SU2 mAb contains a sequence of 6 amino acids (DSDVGE) at position 41-46 of the beta-chain of HLA-DR/DP. Functional studies indicated that SU2 mAb strongly induced cell aggregation and large clumping in resting tonsillar B cells. SU2 mAb inhibited the spontaneous growth and proliferation of B lymphoblastoid cell lines and drastically inhibited [3H]thymidine uptake by phorbol 12-myristate 13-acetate (PMA)-activated resting B cell. Results are discussed in relation to the dual recognition of HLA-DR/DP by SU2 mAb.
Ghaderi
A A
AA
Department of Immunology and Microbiology, Medical School, Shiraz University of Medical Science, Iran.
Gaudernack
G
G
Stanworth
D R
DR
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001402:B-Lymphocytes / Q000502:physiology*; D002449:Cell Aggregation / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D000939:Epitopes / Q000276:immunology*; D006682:HLA-DP Antigens / Q000276:immunology*; D006684:HLA-DR Antigens / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D014066:Palatine Tonsil / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate
D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D000939:Epitopes; D006682:HLA-DP Antigens; D006684:HLA-DR Antigens; D013755:Tetradecanoylphorbol Acetate