[Simplified vibriocidal antibody titer performed on a drop of blood].
We report a simplified technique for vibriocidal antibody test in underprivileged people in tropical area. The test is performed on a drop of blood sampled on a disc of blotting paper. It avoid taking of large quantities of blood in timid underfed people who are often solicited. Its reading is rapid and easy by the naked eye owing to staining viable germs violet by the neotetrazolium succinate. Tested in mice and humans, the simplified method gave data well correlated to those obtained with the standard test on serum for vibriocidal titres higher than (or equal to) 1/40.
Bougoudogo
F
F
Unité du Choléra et des Vibrions, Institut Pasteur, Paris.
Fournier
J M
JM
Guillou
M
M
Dodin
A
A
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002771:Cholera / Q000175:diagnosis; D006801:Humans; D051379:Mice; D010209:Paper; D013194:Staining and Labeling; D013778:Tetrazolium Salts; D014734:Vibrio cholerae / Q000276:immunology*
D000907:Antibodies, Bacterial; D013778:Tetrazolium Salts; C011314:neotetrazolium