Tyrosine phosphorylation of Shc is induced by IL-3, IL-5 and GM-CSF.
The receptors for IL-3, IL-5 and GM-CSF belong to the hematopoietic receptor superfamily and have no intrinsic tyrosine kinase activity but nevertheless indirectly induce protein tyrosine phosphorylation. In order to directly compare the effects of IL-3, IL-5 and GM-CSF on protein tyrosine phosphorylation we analyzed the murine cell line FDCP-1 which proliferates equally well in response to IL-3 and GM-CSF and the cell line B13 which responds to both IL-3 and IL-5. The protein tyrosine phosphorylation pattern induced by IL-3, IL-5 and GM-CSF in these cell lines was shown to be remarkably similar and all three cytokines induced tyrosine phosphorylation of Shc, a src homology domain-2 containing protein which has been shown to be involved in Ras activation by tyrosine kinase receptors.
Dorsch
M
M
Institut für Immunologie, Universitätsklinikum Steglitz, Freie Universität Berlin, Germany.
Hock
H
H
Diamantstein
T
T
D000818:Animals; D015153:Blotting, Western; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004790:Enzyme Induction; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D015848:Interleukin-5 / Q000494:pharmacology*; D007700:Kinetics; D051379:Mice; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism*; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D013936:Thymidine / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
D007377:Interleukin-3; D015848:Interleukin-5; D010750:Phosphoproteins; D011994:Recombinant Proteins; D019000:Phosphotyrosine; D014443:Tyrosine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D011505:Protein-Tyrosine Kinases; D013936:Thymidine