Reactivity of lung tumors with lung-derived and non-lung-derived monoclonal antibodies.
The reactivity of 74 lung-derived monoclonal antibodies (MAbs) provided by the Third International Workshop on Lung Tumor Antigens and of 41 non-lung-derived commercially available MAbs against sections of 15 lung tumors of various histologic types was investigated by immunohistochemistry. Three MAbs with specificity for human neural-cell adhesion molecule (H-NCAM) and 3 MAbs with specificity for small-cell lung carcinoma (SCLC) were able to distinguish between neuro-endocrine (NE) and non-NE tumors. Fifteen MAbs stained non-small-cell carcinomas (NSCLC) but not SCLC. Neuron-specific enolase (NSE) stained all NE tumors but also some of the non-NE tumors. Two MAbs showed specificity for mesotheliomas. Carcino-embryonic MAb strongly stained all SCLC and NSCLC. Among MAbs with lymphoid-cell specificities, Leu 7 (CD57) stained SCLC, but not NSCLC. LN2 (CD45R), LN3 (HLA-DR), Leu 22 (CD43) and BLA 36 reacted with NSCLC and were non-reactive with SCLC. Some of the lung-derived MAbs showed immune staining of lymphoma and melanoma.
Ioachim
H L
HL
Department of Pathology, Lenox Hill Hospital, New York, NY 10021.
Pambuccian
S
S
Giancotti
F
F
Dorsett
B
B
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D018959:CD57 Antigens; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002276:Carcinoid Tumor / Q000145:classification / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000145:classification / Q000473:pathology*; D018288:Carcinoma, Small Cell / Q000145:classification / Q000473:pathology*; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D051917:Leukosialin; D008168:Lung / Q000166:cytology*; D008175:Lung Neoplasms / Q000145:classification / Q000473:pathology*; D008223:Lymphoma / Q000473:pathology; D008545:Melanoma / Q000473:pathology; D018358:Neuroendocrine Tumors / Q000145:classification / Q000473:pathology*; D010751:Phosphopyruvate Hydratase / Q000032:analysis*; D012795:Sialoglycoproteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018959:CD57 Antigens; D002272:Carcinoembryonic Antigen; D015816:Cell Adhesion Molecules, Neuronal; D006684:HLA-DR Antigens; D051917:Leukosialin; C465014:SPN protein, human; D012795:Sialoglycoproteins; D010751:Phosphopyruvate Hydratase