[The diagnosis of hepatocellular carcinoma determined by pattern of AFP bands separated by Con A affinity electrophoresis].
We analyzed the Con A affinity of serum AFP in patients with a serum AFP concentration greater than 50ng/ml by antibody affinity electrophoresis and Western blotting to distinguish hepatocellular carcinoma (HCC) from benign chronic liver diseases (CLD). Of 164 patients with HCC, 48 (29.3%) had a single band, while 116 (70.7%) had multiple bands. All but three of 65 patients with cirrhosis had a single band. All but one of 32 patients with chronic hepatitis had a single band. We concluded that multiple AFP bands are diagnosis of HCC. This method is a useful assay for distinguishing HCC from CLD.
Saito
S
S
Department of Gastroenterology, Toranomon Hospital.
Ikeda
K
K
Koida
I
I
Tsubota
A
A
Arase
Y
Y
Chayama
K
K
Kanda
M
M
Kobayashi
M
M
Kumada
H
H
D000328:Adult; D000368:Aged; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis*; D002908:Chronic Disease; D003208:Concanavalin A; D003937:Diagnosis, Differential; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D005260:Female; D006505:Hepatitis / Q000175:diagnosis; D006801:Humans; D008103:Liver Cirrhosis / Q000175:diagnosis; D008113:Liver Neoplasms / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D000509:alpha-Fetoproteins / Q000032:analysis*
D000509:alpha-Fetoproteins; D003208:Concanavalin A