The DA6-147 monoclonal antibody raised against the HLA-DR alpha chain identifies a cryptic epitope on the BoLA-DR alpha chain.
By combining immunoprecipitation of BoLA molecules with monoclonal antibodies raised against 2 different major histocompatibility class II antigens, TH14B and TH81A, and western blotting using the anti-HLA DR alpha monoclonal antibody DA6-147, we characterized an epitope conserved on BoLA- and HLA-DR alpha chains. This epitope, not accessible on intact cells, was revealed after cell lysis. In addition, these results allowed us to define TH14B as an anti-BoLA-DR monoclonal antibody whereas TH81A, raised against the products of a second MHC locus, is probably an anti-BoLA-DQ monoclonal antibody.
Ababou
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URA-INRA, Immunopathologie Cellulaire et Moléculaire, Ecole Nationale Vétérinaire, Alfort, France.
Davis
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WC
Levy
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D006684:HLA-DR Antigens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000737:chemistry / Q000276:immunology*; D008228:Lymphoma, Non-Hodgkin / Q000473:pathology / Q000662:veterinary; D011233:Precipitin Tests; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II