Anti-(glioma surface antigen) monoclonal antibody G-22 recognizes overexpressed CD44 in glioma cells.
We raised an anti-glioma monoclonal antibody, named G-22, that specifically recognizes a human glioma-associated surface antigen. Proven to be useful for target imaging of malignant gliomas after radioisotope labeling and cerebrospinal fluid diagnosis by enzyme-linked immunospecific assay, G-22 was found to immunoprecipitate an 85-kDa glycoprotein of the human glioma U-251MG cell. We purified this antigen by G-22-coupled cyanogenbromide-activated Sepharose affinity chromatography, and sequence analysis demonstrated that the 54 amino acid residues were identical to positions 55-108 of human CD44. The results show that the smallest spliced form (85 kDa) of CD44 is strongly expressed in glioma cells.
Okada
H
H
Department of Neurosurgery, Nagoya University School of Medicine, Japan.
Yoshida
J
J
Seo
H
H
Wakabayashi
T
T
Sugita
K
K
Hagiwara
M
M
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis*; D002352:Carrier Proteins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005910:Glioma / Q000276:immunology*; D006801:Humans; D018960:Hyaluronan Receptors; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000032:analysis*; D016201:Receptors, Lymphocyte Homing / Q000032:analysis*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing