title: Whole body and splanchnic leucine, phenylalanine, and glucose kinetics during endotoxemia in humans.
abstract: To examine the whole body and splanchnic tissue substrate handling during endotoxemia, an intravenous bolus of endotoxin was given to six healthy volunteers during primed, continuous infusions of [1-13C]leucine, [ring-2H5]phenylalanine, and [6,6-2H2]glucose. Whole body protein breakdown, based on whole body Leu and Phe appearance rates (Ra), increased in response to endotoxin given at time 0 (RaLeu 77 +/- 2 mol.kg-1 x h-1, t = 0 h; 88 +/- 6, t = 4 h; P < 0.05) (RaPhe 39 +/- 2 mol.kg-1 x h-1, t = 0; 46 +/- 3, t = 4 h; P < 0.05). Splanchnic amino acid balance (Bal) increased (BalLeu 7 +/- 4 mol.kg-1 x h-1, t = 0; 21 +/- 5, t = 2 h; P < 0.05) (BalPhe 3 +/- 2 mol.kg-1 x h-1, t = 0; 16 +/- 4, t = 2 h; P < 0.05) and can be accounted for by increased splanchnic uptake (Rd) of Phe and Leu (RdLeu 21 +/- 3 mol.kg-1 x h -1, t = 0; 37 +/- 7, t = 120 min; P < 0.05) (RdPhe 10 +/- 3 mol.kg-1 x h-1, t = 0; 24 +/- 5, t = 120 min; P < 0.05). Splanchnic conversion of Leu to ketoisocaproate increased with endotoxin administration (0.7 +/- 0.6 mol.kg-1 x h-1, t = 0; 8 +/- 3, t = 360 min; P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, New York Hospital-Cornell Medical Center, New York 10021.
authors.forename: Y
authors.initials: Y
authors.lastname: Fong
authors.forename: D E
authors.initials: DE
authors.lastname: Matthews
authors.forename: W
authors.initials: W
authors.lastname: He
authors.forename: M A
authors.initials: MA
authors.lastname: Marano
authors.forename: L L
authors.initials: LL
authors.lastname: Moldawer
authors.forename: S F
authors.initials: SF
authors.lastname: Lowry
mesh_terms: D000209:Acute-Phase Proteins / Q000378:metabolism; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002247:Carbon Isotopes; D003903:Deuterium; D004731:Endotoxins / Q000633:toxicity*; D004926:Escherichia coli; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D007930:Leucine / Q000097:blood / Q000378:metabolism*; D008297:Male; D010649:Phenylalanine / Q000097:blood / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*; D013152:Splanchnic Circulation; D014115:Toxemia / Q000097:blood / Q000378:metabolism*
chemical_list: D000209:Acute-Phase Proteins; D001786:Blood Glucose; D002247:Carbon Isotopes; D004731:Endotoxins; D011506:Proteins; D010649:Phenylalanine; D003903:Deuterium; D007930:Leucine; D005947:Glucose