Modulation of the growth of hematopoietic human stem cells by growth factors: comparison of umbilical cord blood with bone marrow.
The growth of cord blood and bone marrow myeloid mononuclear cells in response to single and multiple growth factors has been examined. Single factors such as IL-3, IL-6, GM-CSF, and stem cell factor did not optimally stimulate growth of cord blood CFU-GM. However a synergistic stimulation was observed when the factors were combined. This effect was seen in both the presence and absence of added fetal calf serum.
DeBruyn
C
C
Service de Médecine Interne, Clinique Henri Tagnon, Institut J. Bordet, Brussels, Belgium.
Stryckmans
P
P
Bron
D
D
Ley
P
P
Degueldre
M
M
Delforge
A
A
D015703:Antigens, CD; D018952:Antigens, CD34; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D003973:Diatrizoate; D005312:Fetal Blood / Q000166:cytology*; D005362:Ficoll; D005780:Gelatin; D006412:Hematopoietic Stem Cells; D006801:Humans; D007231:Infant, Newborn; D008747:Methylcellulose; D011205:Povidone; D012822:Silicon Dioxide
D015703:Antigens, CD; D018952:Antigens, CD34; D003973:Diatrizoate; D005362:Ficoll; C016039:Percoll; D012822:Silicon Dioxide; D005780:Gelatin; D008747:Methylcellulose; D011205:Povidone