Calcium currents recorded from a neuronal alpha 1C L-type calcium channel in Xenopus oocytes.
Xenopus oocytes expressing neuronal alpha 1C, alpha 2 and beta 1b calcium channel subunit cDNAs were used in this study. During two-electric voltage clamp recording the oocyte was injected with 10-20 nl of a 100 mM BAPTA solution. Under these conditions, the endogenous Ca-activated Cl current was completely suppressed resulting in an alpha 1C Ba current free from Cl current contamination. BAPTA injection also allowed alpha 1C currents with different permeating ions, including Ca, to be examined. Compared to Ba and Sr, alpha 1C whole cell Ca currents were smaller in magnitude and showed kinetic and voltage-dependent properties more similar to those for L-type Ca currents recorded in native cells. That Ca-dependent inactivation occurs in BAPTA-buffered cells suggests that the Ca-binding site involved in this type of inactivation is very close to the pore of the channel.
Charnet
P
P
CRBM-CNRS UPR 9008, INSERM U249, Montpellier, France.
Bourinet
E
E
Dubel
S J
SJ
Snutch
T P
TP
Nargeot
J
J
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D001464:Barium / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D015220:Calcium Channels / Q000235:genetics / Q000502:physiology*; D018118:Chloride Channels / Q000187:drug effects / Q000502:physiology; D018076:DNA, Complementary / Q000235:genetics; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D018014:Gene Transfer Techniques; D007700:Kinetics; D009474:Neurons / Q000502:physiology*; D009865:Oocytes / Q000502:physiology*; D011994:Recombinant Proteins; D014982:Xenopus laevis
D015220:Calcium Channels; D018118:Chloride Channels; D018076:DNA, Complementary; D011994:Recombinant Proteins; D001464:Barium; D004533:Egtazic Acid; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; C025603:1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid; D002118:Calcium