title: Retrovirus tests of human leukemia/lymphoma cell lines at DSM.
abstract: Permanently established human cell lines can produce several retroviruses. It is important to routinely test such cell lines for human T cell lymphotropic virus (HTLV) type I and II, and for human immunodeficiency virus (HIV) type 1 and 2 in order to exclude any potential biohazard from cell lines producing human retroviruses. Reverse transcriptase assay, polymerase chain reaction, and dot-blot hybridization of in-vitro amplified DNA with virus-specific probes are used.
authors.affiliation: German Collection of Microorganisms and Cell Cultures (DSM), Braunschweig, Germany.
authors.forename: B G
authors.initials: BG
authors.lastname: Häne
authors.forename: H G
authors.initials: HG
authors.lastname: Drexler
mesh_terms: D003264:Containment of Biohazards; D004279:DNA, Viral / Q000032:analysis; D017977:Deltaretrovirus / Q000302:isolation & purification*; D006678:HIV / Q000302:isolation & purification*; D006801:Humans; D007938:Leukemia / Q000382:microbiology*; D008223:Lymphoma / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D004279:DNA, Viral; D012194:RNA-Directed DNA Polymerase