title: Excitatory amino acid-induced excitation of dopamine-containing neurons in the rat substantia nigra: modulation by kynurenic acid.
abstract: Kynurenic acid (KYNA), an endogenous antagonist of ionotropic excitatory amino acid (EAA) receptors, was tested for its ability to modulate N-methyl-D-aspartate (NMDA)- and alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)-induced excitation of dopamine (DA)-containing neurons in the zona compacta of the rat substantia nigra (SNc). Experiments were conducted using extracellular recording techniques in conjunction with an in vitro brain slice preparation. Bath application of NMDA (1-20 microM) or AMPA (0.5-10 microM) produced a concentration-dependent increase in the firing rate of SNc DA neurons but had no effect on firing pattern. The highest concentration of both agonists produced a rapid and reversible cessation of activity that was attributed to acute induction of depolarization block. Addition of glycine (GLY) (up to 100 microM) to the bathing solution had no effect on either basal firing rate or the increase in activity produced by NMDA. KYNA (10 microM-1 mM) antagonized the excitatory effects of both NMDA (15 microM) and AMPA (3 microM) in a concentration-dependent fashion (IC50:102 microM and 64 microM, respectively) without affecting basal firing rate. Perfusion of tissue slices with a modified Ringer's solution containing low Mg2+ (0.12 mM) increased NMDA-induced excitation but did not affect the antagonist properties of KYNA. D-serine (100 microM) reversed the ability of KYNA to block the excitatory effects of NMDA, suggesting that KYNA attenuates NMDA-induced excitation of SNc DA neurons via blockade of the GLY allosteric site on the NMDA receptor. The ability of KYNA to modulate the excitatory effects of both NMDA and non-NMDA agonists implies that endogenous KYNA may play a physiological role in regulating DA cell excitability.
authors.affiliation: Maryland Psychiatric Research Center, University of Maryland School of Medicine, Baltimore 21228.
authors.forename: H Q
authors.initials: HQ
authors.lastname: Wu
authors.forename: R
authors.initials: R
authors.lastname: Schwarcz
authors.forename: P D
authors.initials: PD
authors.lastname: Shepard
mesh_terms: D000818:Animals; D004298:Dopamine / Q000378:metabolism*; D004594:Electrophysiology; D005110:Extracellular Space / Q000502:physiology; D066298:In Vitro Techniques; D007736:Kynurenic Acid / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D016202:N-Methylaspartate / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013378:Substantia Nigra / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
chemical_list: D016202:N-Methylaspartate; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D007736:Kynurenic Acid; D008274:Magnesium; D004298:Dopamine