Identification of sperm antigen targets for immunocontraception: B-cell epitope analysis of Sp17.
The selection of immunological targets present on gametes is an important first step in the successful production of animal or human vaccines for immunocontraception. One strategy with regard to sperm antigens is to select antigens with physiological roles in gamete interaction, obtain the mRNA sequence for such an antigen, and then determine which region or domain of the molecule is available to the immune system when spermatozoa are presented to the female reproductive system. To illustrate this strategy, we have used the rabbit sperm antigen Sp17 (RSA-3), which has been cloned and sequenced. The peptide pin-block method of Chiron Mimotopes, Australia, has been used to analyse the 146 amino acids of Sp17 with various homologous and heterologous antisera. This study demonstrates that such an analysis can lead to the identification of a B-cell epitope with strong immunocontraceptive potential.
O'Rand
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MG
Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599.
Widgren
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EE
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D003269:Contraception, Immunologic; D000939:Epitopes / Q000737:chemistry*; D005260:Female; D007301:Insect Hormones / Q000276:immunology*; D019476:Insect Proteins; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011817:Rabbits; D013094:Spermatozoa / Q000276:immunology*; D014612:Vaccines
D000939:Epitopes; D007301:Insect Hormones; D019476:Insect Proteins; C085315:Sp17 protein, Chironomus tentans; D014612:Vaccines