title: Structural studies of the O-antigenic polysaccharides of Escherichia coli O3 and the enteroaggregative Escherichia coli strain 17-2.
abstract: The polysaccharide part of the lipopolysaccharide isolated from an enteroaggregative Escherichia coli isolated from a young child with diarrhoea in Santiago, Chile (strain 17-2), has been investigated. Sugar and methylation analyses of native and partially degraded polysaccharide together with 1H-NMR and 13C-NMR spectroscopies revealed that the polysaccharide is composed of pentasaccharide repeating units. The structure of the repeating unit of E. coli strain 17-2 O-polysaccharide is: [formula: see text] The structure of the O-polysaccharide from E. coli O3 was shown to be identical to that of E. coli strain 17-2 by sugar and methylation analyses and by 1H-NMR and 13C-NMR spectroscopies.
authors.affiliation: Department of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institute, Sweden.
authors.forename: E C
authors.initials: EC
authors.lastname: Medina
authors.forename: G
authors.initials: G
authors.lastname: Widmalm
authors.forename: A
authors.initials: A
authors.lastname: Weintraub
authors.forename: P A
authors.initials: PA
authors.lastname: Vial
authors.forename: M M
authors.initials: MM
authors.lastname: Levine
authors.forename: A A
authors.initials: AA
authors.lastname: Lindberg
mesh_terms: D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry*
chemical_list: D019081:O Antigens; D011135:Polysaccharides, Bacterial