[Cloning and characterization of a specific cytokeratin-8 cDNA from rat prostatic epithelium].
In this study, we report the cloning and sequencing of a full-length cytokeratin-8 (CK-8) cDNA from a rat prostatic epithelial cDNA library. The effects of androgen on CK-8 expression in rat prostate were also studied. The data indicated that the steady-state level of CK-8 mRNA was elevated by about 5- to 10-fold in both prostate and seminal vesicles in castrated rats, and the elevated levels persisted during a 23-day experimental period. Androgen but not estrogen administration repressed the expression of CK-8 mRNA. This effect could be antagonized by the simultaneous administration of an antiandrogen, flutamide, indicating that CK-8 is a new class of androgen-repressed genes whose regulation is presumably mediated by androgen receptor mechanisms.
Wang
X
X
Guangzhou General Hospital of PLA.
Hsieh
J T
JT
Zhau
H E
HE
D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D004847:Epithelial Cells; D005485:Flutamide / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D007633:Keratins / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D011467:Prostate / Q000166:cytology*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D000728:Androgens; D018076:DNA, Complementary; D012333:RNA, Messenger; D007633:Keratins; D005485:Flutamide