title: Induction of chromosomal aberrations with benzon nuclease in Chinese hamster ovary (CHO) cells.
abstract: Benzon nuclease, an endonuclease originating from Serratia marcescens, was tested for its chromosome breaking activity in Chinese hamster ovary cells. Using a permeabilizing method with hypertonic glycerol, benzon nuclease induced chromosomal aberrations in an S-phase independent manner. The frequencies of polycentric chromosomes were correlated with the dose of the enzyme and the intercellular distribution of aberrations was overdispersed.
authors.affiliation: University GH Essen, Department of Genetics, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Johannes
authors.forename: G
authors.initials: G
authors.lastname: Obe
mesh_terms: D000818:Animals; D016466:CHO Cells; D002869:Chromosome Aberrations; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004720:Endonucleases / Q000633:toxicity*; D016196:S Phase; D012706:Serratia marcescens / Q000201:enzymology
chemical_list: D004720:Endonucleases