title: Conduction abnormalities are restricted to the central nervous system in experimental autoimmune encephalomyelitis induced by inoculation with proteolipid protein but not with myelin basic protein.
abstract: Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disease of the central nervous system (CNS) and can be induced by inoculation of animals with homogenized CNS tissue or highly purified myelin proteins such as myelin basic protein (MBP) or proteolipid protein (PLP). It is widely studied as a possible animal model of multiple sclerosis. We performed the present neurophysiological study to define the location of nerve conduction abnormalities in EAE induced by immunization with PLP (PLP-EAE) and in EAE induced by immunization with MBP (MBP-EAE) in the Lewis rat. In rats with tail weakness due to acute PLP-EAE, conduction was normal in the spinal nerve roots and peripheral nerves but there was evidence of conduction block in a high proportion of the fibres in the dorsal columns of the lumbosacral spinal cord. In contrast, in acute MBP-EAE, there was conduction block in a high proportion of fibres in the sacral dorsal and ventral roots of the peripheral nervous system (PNS) and in the dorsal columns of the lumbosacral spinal cord. The distribution of nerve conduction abnormalities is consistent with previous histological studies showing that inflammation and primary demyelination are restricted to the CNS in PLP-EAE, but are present in the CNS and in the spinal roots of the PNS in MBP-EAE. The restriction of functional abnormalities to the CNS in PLP-EAE but not in MBP-EAE may have implications for the human inflammatory demyelinating diseases, including multiple sclerosis.
authors.affiliation: Department of Medicine, University of Queensland, Royal Brisbane Hospital, Australia.
authors.forename: J B
authors.initials: JB
authors.lastname: Chalk
authors.forename: P A
authors.initials: PA
authors.lastname: McCombe
authors.forename: M P
authors.initials: MP
authors.lastname: Pender
mesh_terms: D000200:Action Potentials; D000818:Animals; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D009431:Neural Conduction; D051381:Rats; D013116:Spinal Cord / Q000473:pathology / Q000503:physiopathology; D013126:Spinal Nerve Roots / Q000503:physiopathology*
chemical_list: D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein