Enumeration of Vibrio vulnificus on membrane filters with a fluorescently labeled oligonucleotide probe specific for kingdom-level 16S rRNA sequences.
Vibrio vulnificus was enumerated on membrane filters after hybridization with a fluorescent oligonucleotide eubacterial probe. Cells were hybridized in liquid buffer or directly on membrane filters. There was no significant difference between fluorescent oligonucleotide direct counts and acridine orange direct counts (P > 0.05). Liquid buffer hybridization was preferable to direct filter hybridization.
Heidelberg
J F
JF
Marine-Estuarine-Environmental Science Program, University of Maryland, College Park 20742.
O'Neill
K R
KR
Jacobs
D
D
Colwell
R R
RR
D000165:Acridine Orange; D001483:Base Sequence; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics; D019793:Fluorescein; D005452:Fluoresceins; D008851:Micropore Filters; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014733:Vibrio / Q000235:genetics* / Q000302:isolation & purification*
D004269:DNA, Bacterial; D005452:Fluoresceins; D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D000165:Acridine Orange; D019793:Fluorescein