Production of monoclonal antibodies against Xanthomonas campestris pv. mangiferaeindicae and their use to investigate differences in virulence.
Four Xanthomonas campestris pv. mangiferaeindicae isolates from mango black spot lesions were grouped according to differences in virulence and used to raise monoclonal antibodies (mAbs). Two immunization approaches were followed. In the first, four groups of mice were immunized, each with a different isolate and the spleens from each group homogenized together for cell fusion. The second approach entailed immunization of a single group of mice with bacteria pooled from all four isolates. The resultant mAbs were characterized with regard to the antigen binding specificity and antibody class. A relationship between mAb binding specificity and virulence of the bacteria was shown by Western blot analysis.
Sanders
G M
GM
Department of Microbiology and Plant Pathology, University of Pretoria, South Africa.
Verschoor
J A
JA
van Wyngaard
S
S
Korsten
L
L
Kotzé
J M
JM
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005638:Fruit / Q000382:microbiology*; D007074:Immunoglobulin G / Q000145:classification; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010935:Plant Diseases / Q000382:microbiology*; D014774:Virulence; D016959:Xanthomonas campestris / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G