Myelin protein transcripts increase in experimental diabetic neuropathy.
A Northern blot analysis of P0 and MBP myelin protein transcripts in the sciatic nerve from rats with alloxan-induced diabetes at two different time points is described. After 5 weeks of diabetes induction, only P0 mRNA is significantly increased by 39%, while at 14 weeks both P0 and MBP mRNA contents are markedly higher than controls. Insulin treatment normalizes glycemia levels, partially counteracts P0 mRNA increase at both stages of diabetes and delays MBP mRNA increase present only in chronic animals. We suggest that increased transcript levels of P0 and MBP in Schwann cells may represent a higher turnover of myelin sheath specific proteins in diabetic syndrome, as attempt to repair the hyperglycemia-induced nerve damage, which is partially prevented by insulin treatment.
Conti
A M
AM
Institute of Neurology, Dino Ferrari Center, University of Milan, Italy.
Malosio
M L
ML
Scarpini
E
E
Di Giulio
A M
AM
Scarlato
G
G
Mantegazza
P
P
Gorio
A
A
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015152:Blotting, Northern; D001835:Body Weight / Q000502:physiology; D018076:DNA, Complementary / Q000096:biosynthesis; D003720:Densitometry; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D007328:Insulin / Q000494:pharmacology; D008297:Male; D004676:Myelin Basic Protein / Q000096:biosynthesis / Q000276:immunology; D009185:Myelin Proteins / Q000096:biosynthesis* / Q000276:immunology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000378:metabolism / Q000473:pathology; D014158:Transcription, Genetic; D015854:Up-Regulation / Q000187:drug effects
D001786:Blood Glucose; D018076:DNA, Complementary; D007328:Insulin; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D012333:RNA, Messenger