A polymerase chain reaction-based enzyme-linked immunosorbent assay for relative quantitation of T cell receptor beta-gene expression. Its application to the analysis of T cell receptor repertoire usage in alloreactive responses.
Shirwan
H
H
Department of Surgery, Cedars-Sinai Research Institute, Cedars-Sinai Medical Center, Los Angeles, California 90048.
Leamer
M
M
Makowka
L
L
Cramer
D V
DV
D000818:Animals; D002478:Cells, Cultured; D015342:DNA Probes; D006825:Hybridomas; D007124:Immunoenzyme Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D012313:RNA / Q000032:analysis; D051381:Rats; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics* / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
D015342:DNA Probes; D016693:Receptors, Antigen, T-Cell, alpha-beta; D012313:RNA