title: Fluorinated proteins as potential 19F magnetic resonance imaging and spectroscopy agents.
abstract: Fluorinated proteins have been synthesized and characterized as potential in vivo 19F magnetic resonance imaging (MRI) and spectroscopy (MRS) agents. Proteins labeled with fluorine include bovine serum albumin, gamma-globulin, and purified immunoglobulin (IgG). The amino groups in proteins were selectively trifluoroacetylated using S-ethyl trifluorothioacetate to synthesize fluorinated proteins (TFA-protein; 1-3). In another approach, trifluoroacetamidosuccinic anhydride has been used to prepare corresponding fluorinated derivatives of proteins (TFASA-protein; 4-6). The fluorinated proteins have been purified by exhaustive dialysis and isolated in good yields (55-76%). The fluorinated proteins exhibit useful NMR characteristics and the biocompatibility for in vivo studies. The initial investigations demonstrate the potential of these new fluorinated proteins as in vivo MRI/MRS probes.
authors.affiliation: Department of Radiology, University of Texas Southwestern Medical Center, Dallas 75235.
authors.forename: V D
authors.initials: VD
authors.lastname: Mehta
authors.forename: P V
authors.initials: PV
authors.lastname: Kulkarni
authors.forename: R P
authors.initials: RP
authors.lastname: Mason
authors.forename: A
authors.initials: A
authors.lastname: Constantinescu
authors.forename: P P
authors.initials: PP
authors.lastname: Antich
mesh_terms: D000818:Animals; D002417:Cattle; D005461:Fluorine / Q000097:blood / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D007700:Kinetics; D046911:Macromolecular Substances; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008961:Models, Structural; D015335:Molecular Probes; D011506:Proteins / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D012710:Serum Albumin, Bovine / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D005719:gamma-Globulins / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D007074:Immunoglobulin G; D046911:Macromolecular Substances; D015335:Molecular Probes; D011506:Proteins; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D005461:Fluorine