Differential steroidogenic response of subpopulations of porcine granulosa cells to insulin-like growth factor-1 (IGF-1) or IGF-1 analogs.
Two experiments were conducted to examine responses of porcine granulosa cells to insulin-like growth factor-1 (IGF-1) or IGF-1 analogs (des [1-3] and Long R3) that have reduced affinity for IGF-binding proteins (IGFBP). Both experiments evaluated estradiol and IGFBP production by granulosa cells after separation of cells into subpopulations that maintain long-term estradiol production in vitro (tightly bound) and those that do not (weakly associated). Granulosa cells were obtained from medium-sized follicles (4-6 mm) at random stages of the estrous cycle in experiment 1 and from the 10 largest follicles per ovary at 0 or 48 h after weaning in experiment 2. Follicle diameter and follicular fluid estradiol concentrations increased with time after weaning (p < 0.05). Tightly bound cells produced more estradiol than weakly associated cells at 24-120 h of culture in experiment 1 and from 0 to 48 h in experiment 2 (p < 0.05). In tightly bound but not weakly associated cells, IGF-1 stimulated estradiol production. The IGF analogs were more potent stimulators than IGF-1 in experiment 1 (p < 0.05); and in experiment 2, this response was restricted to cells collected at 48 h after weaning. Conversely, tightly bound cells obtained at 0 h after weaning responded similarly to IGF-1 and des (1-3). During the final 48 h of culture, weakly associated cells produced greater quantities of 28-30-kDa IGFBP than did tightly bound cells in response to IGF-1 or analogs (both experiments; p < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
Howard
H J
HJ
U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933-0166.
Ford
J J
JJ
D000818:Animals; D002352:Carrier Proteins / Q000096:biosynthesis; D002469:Cell Separation; D002478:Cells, Cultured; D004958:Estradiol / Q000096:biosynthesis*; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018969:Insulin-Like Growth Factor Binding Proteins; D007334:Insulin-Like Growth Factor I / Q000031:analogs & derivatives* / Q000494:pharmacology*; D007700:Kinetics; D006080:Ovarian Follicle / Q000033:anatomy & histology; D013552:Swine
D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D004958:Estradiol; D007334:Insulin-Like Growth Factor I