title: Immunohistochemical studies with skin mast cells.
abstract: Using different histochemical and immunohistochemical methods we demonstrate that isolated skin mast cells used for different pharmacological and biochemical studies exhibit the same staining pattern compared to skin mast cells in situ. Surface structures and enzyme content of the cells appear not to be influenced by the isolation technique. We also observed no differences in the staining pattern of mast cells in neurofibroma, a cutaneous disorder characterized by an increase of mast cell numbers.
authors.affiliation: Department of Dermatology, Medical University of Lübeck, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Schmolke
authors.forename: U
authors.initials: U
authors.lastname: Amon
authors.forename: N
authors.initials: N
authors.lastname: Zemcke
authors.forename: H H
authors.initials: HH
authors.lastname: Wolff
mesh_terms: D053818:Chymases; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008407:Mast Cells / Q000201:enzymology / Q000502:physiology*; D017253:Neurofibromatoses / Q000473:pathology; D010447:Peptide Hydrolases / Q000378:metabolism; D012697:Serine Endopeptidases / Q000378:metabolism; D012867:Skin / Q000166:cytology*; D013194:Staining and Labeling
chemical_list: D007073:Immunoglobulin E; D010447:Peptide Hydrolases; C023073:tosylarginine methyl ester hydrolase; D012697:Serine Endopeptidases; D053818:Chymases