Evaluation of possible histamine release from human peripheral blood cells using an enzyme immunoassay (HRT) with components of intravenous catheters.
The HRT Assay was evaluated for its capacity to measure histamine release from blood basophils following the introduction of extracts from catheters made of Aquavene or silicone. Blood samples were collected from twenty-one volunteers of the University of California, Davis campus and from seven individual who had experienced a systemic event during the insertion of catheters made from Aquavene. None of the blood samples released histamine in quantities that would be anticipated in an anaphylactic or anaphylactoid reaction when challenged with the extracts; all released histamine when challenged with polymyxin B, used as a positive control. Based upon these results, none of the components of either the Aquavene-based or silicone-based catheters are thought to cause a histamine-associated reaction in subjects. This assay proved to be both expedient and reliable in its determination of the release of histamine from blood basophils.
Leung
P S
PS
Department of Internal Medicine, University of California Davis School of Medicine 95616.
Halpern
G M
GM
Gershwin
M E
ME
D000707:Anaphylaxis / Q000209:etiology*; D001491:Basophils / Q000187:drug effects / Q000378:metabolism*; D001672:Biocompatible Materials / Q000009:adverse effects / Q000494:pharmacology; D002406:Catheterization, Peripheral / Q000009:adverse effects* / Q000295:instrumentation; D004797:Enzyme-Linked Immunosorbent Assay; D005782:Gels / Q000009:adverse effects / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D020100:Hydrogels; D011112:Polymyxin B / Q000494:pharmacology; D012828:Silicones / Q000009:adverse effects / Q000494:pharmacology
C085051:Aquavene; D001672:Biocompatible Materials; D005782:Gels; D020100:Hydrogels; D012828:Silicones; D011112:Polymyxin B