High-performance liquid chromatographic determination of benzalkonium and naphazoline or tetrahydrozoline in nasal and ophthalmic solutions.
A high-performance liquid chromatographic method is described for the determination of benzalkonium chloride homologues and naphazoline nitrate or tetrahydrozoline hydrochloride in ophthalmic and nasal solutions. The technique involves a one-step dilution (1:5) for sample preparation and direct injection onto a 5 mm RP C-8 column. The mobile phase is acetonitrile-diluted acetic acid (80:20, v/v) with 6 mM tetramethylammonium bromide. Detection is carried out at 260 nm with a diode array. The method is rapid, specific, reproducible and is especially useful for quality control procedures.
Santoni
G
G
Stabilimento Chimico Farmaceutico Militare, Firenze, Italy.
Medica
A
A
Gratteri
P
P
Furlanetto
S
S
Pinzauti
S
S
D001548:Benzalkonium Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D007093:Imidazoles / Q000032:analysis*; D009278:Naphazoline / Q000032:analysis*; D014663:Nasal Decongestants / Q000032:analysis*; D009883:Ophthalmic Solutions; D012996:Solutions
D001548:Benzalkonium Compounds; D007093:Imidazoles; D014663:Nasal Decongestants; D009883:Ophthalmic Solutions; D012996:Solutions; D009278:Naphazoline; C005810:tetrahydrozoline