title: Differentiation of Salmonella serovar infantis isolates from human and animal sources by fingerprinting IS200 and 16S rrn loci.
abstract: We genotyped Salmonella serovar infantis (referred to as S. infantis), which is the most widespread serovar among animals and the third most common cause of human salmonellosis in Finland. Molecular fingerprinting of the 16S rrn locus and the Salmonella-specific insertion sequence IS200 was used to type the 131 isolates originating from the main sources of S. infantis infection. The number of IS200 elements in S. infantis varied from zero to seven; three or more copies were present in 97% of the isolates, and 71% had four copies. There were four conserved chromosomal positions of IS200, which allowed us to group the isolates into three major clonal groups. We defined 11 unique IS200 profiles and five different ribotypes which, in combination, generated 15 genotypes highly restricted to the infection sources: 8 genotypes were typical of isolates from broiler chickens and cattle and seven genotypes were typical of isolates from humans. The eight genotypes of isolates from chickens represented two clonal groups which were differentially associated with chicken-producing companies. The typing scheme allows efficient discrimination between isolates from various infection sources and within sources and, therefore, provides a unique molecular tool for use in the study of the epidemiology of S. infantis infection.
authors.affiliation: Regional Laboratory of Kuopio, National Veterinary and Food Research Institute, Finland.
authors.forename: S
authors.initials: S
authors.lastname: Pelkonen
authors.forename: E L
authors.initials: EL
authors.lastname: Romppanen
authors.forename: A
authors.initials: A
authors.lastname: Siitonen
authors.forename: J
authors.initials: J
authors.lastname: Pelkonen
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000382:microbiology; D002418:Cattle Diseases / Q000382:microbiology; D002645:Chickens / Q000382:microbiology; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D004196:Disease Outbreaks; D005387:Finland / Q000453:epidemiology; D006801:Humans; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000382:microbiology; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012475:Salmonella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012478:Salmonella Food Poisoning / Q000453:epidemiology / Q000382:microbiology; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012680:Sensitivity and Specificity; D012703:Serotyping
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S