The lamin B receptor-associated protein p34 shares sequence homology and antigenic determinants with the splicing factor 2-associated protein p32.
The lamin B receptor (p58) is an inner nuclear membrane protein that forms an in vivo complex with the nuclear lamins, a nuclear envelope kinase, and two other nuclear proteins with apparent M(r) of 18,000 (p18) and 34,000 (p34). We now report the isolation of p34 by partial dissociation of the immunoaffinity-purified p58 protein complex. Determination of the N-terminal amino acid sequence of purified p34 shows that this polypeptide is homologous to p32, a splicing factor 2 (SF2)-associated protein. The relatedness between p34 and p32 can be further established by showing that antibodies raised against N- and C-terminal peptides of p32 cross-react with purified p34. As the amino acid sequence of p58 contains an arginine/serine (RS)-rich region similar to the RS-rich region found in SF 2, we speculate that these domains provide binding sites for p34 and that this protein may be a linker between the nuclear membrane and intranuclear spliceosomal substructures.
Simos
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Programme of Cell Biology, European Molecular Biology Laboratory, Heidelberg, Germany.
Georgatos
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D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D000939:Epitopes / Q000737:chemistry; D004912:Erythrocytes / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009687:Nuclear Proteins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D016601:RNA-Binding Proteins; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D000068103:Serine-Arginine Splicing Factors; D014422:Turkeys / Q000097:blood; D000097682:Lamin B Receptor
D000939:Epitopes; D008565:Membrane Proteins; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D000068103:Serine-Arginine Splicing Factors; D000097682:Lamin B Receptor