Enhanced immunogenicity of leucine enkephalin following coupling to anti-immunoglobulin and anti-CD3 antibodies.
Leucine enkephalin (Leu-enk) was coupled to both T and B cell antibodies in order to investigate the possibility of enhanced immunogenicity via targeted immunization. The two antibodies used were Hm x Mo CD3 and Gt x Mo Ig, respectively. The data indicate that while both antibody carriers enhanced the immunogenicity of Leu-enk, the use of the Hm x Mo CD3 antibody resulted in a greater number of mice with positive Leu-enk specific serum titers. 12 Leu-enk cell lines were produced and one, LE4H8, was chosen for characterization.
Hendrickson
T L
TL
Department of Chemistry, University of Kansas, Lawrence 66045.
Wilson
G S
GS
Frazer
J M
JM
Liu
R S
RS
McCormack
R T
RT
Wolfert
R L
RL
D000595:Amino Acid Sequence; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D000915:Antibody Affinity; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D017252:CD3 Complex / Q000276:immunology* / Q000378:metabolism; D004743:Enkephalin, Leucine / Q000276:immunology* / Q000378:metabolism*; D000939:Epitopes / Q000032:analysis; D005260:Female; D007700:Kinetics; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008969:Molecular Sequence Data; D013268:Stimulation, Chemical; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D017252:CD3 Complex; D000939:Epitopes; C048844:anti-IgG; C044203:anti-IgM; D004743:Enkephalin, Leucine