Melanocyte lineage-specific antigens recognized by monoclonal antibodies NKI-beteb, HMB-50, and HMB-45 are encoded by a single cDNA.
The glycoproteins recognized by monoclonal antibody (MAb) NKI-beteb are among the best diagnostic markers for human melanoma. MAb NKI-beteb reacts with melanoma cells throughout tumor development and does not cross-react with other tumor or normal cells, except for cells of the melanocytic lineage. Two other melanocyte lineage-specific MAbs, HMB-50 and HMB-45, show a specificity and staining pattern strikingly similar to the ones observed for NKI-beteb. Herein, we demonstrate that all three MAbs recognize protein products encoded by a single cDNA. Expression of this cDNA in BLM cells results in immunoreactivity with all three MAbs. In addition, we demonstrate co-distribution of the RNA species detected by the cDNA with the proteins recognized by the MAbs in tissue sections.
Adema
G J
GJ
Division of Immunology, The Netherlands Cancer Institute, Antoni van Leeuwenhoek Huis, Amsterdam.
de Boer
A J
AJ
van 't Hullenaar
R
R
Denijn
M
M
Ruiter
D J
DJ
Vogel
A M
AM
Figdor
C G
CG
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000032:analysis; D015152:Blotting, Northern; D002460:Cell Line; D018076:DNA, Complementary / Q000032:analysis* / Q000235:genetics; D000939:Epitopes / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008544:Melanocytes / Q000166:cytology* / Q000276:immunology*; D008545:Melanoma / Q000276:immunology* / Q000473:pathology*; D012313:RNA / Q000032:analysis / Q000235:genetics; D012334:RNA, Neoplasm / Q000032:analysis / Q000235:genetics; D012867:Skin / Q000166:cytology / Q000276:immunology / Q000473:pathology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D000939:Epitopes; D012334:RNA, Neoplasm; D012313:RNA