Three-dimensional mammalian cell growth on nonwoven polyester fabric disks.
Small disks of nonwoven polyester fabric (NWPF), similar to Fibra-Cel carriers, were surface treated with strong acid and used as a high surface area support matrix for in vitro culture of anchorage dependent MRC-5 cells. The disks can be autoclaved, and require inocula concentrations of only 2-5% of the final cell densities, which have reached 10(8) cells/mL of disk bed volume. Scanning electron microscopy photographs shown herein reveal that cells grow in a multilayered fashion between the randomly arrayed fibers of the nonwoven fabric, emulating in vivo growth.
Petti
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Department of Chemical Engineering, Tufts University, Medford, Massachusetts 02155.
Lages
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Sussman
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MV
D002448:Cell Adhesion; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D005347:Fibroblasts / Q000166:cytology*; D006416:Hematoxylin; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D013194:Staining and Labeling
D003470:Culture Media; D011091:Polyesters; D006416:Hematoxylin