Usefulness of gram staining of blood collected from total parenteral nutrition catheter for rapid diagnosis of catheter-related sepsis.
The accuracy of Gram staining of blood drawn from catheters used to administer total parenteral nutrition was compared with paired quantitative blood cultures for the diagnosis of catheter-related sepsis. Gram staining was positive in 11 of 18 episodes of catheter-related sepsis documented by quantitative culture (sensitivity, 61%) but in none of the 5 episodes of fever unrelated to catheter infection. Thus, this procedure enabled the rapid presumptive diagnosis and guidance of antimicrobial therapy for total parenteral nutrition catheter sepsis, with a positive predictive value of 100% and a negative predictive value of 42%.
Moonens
F
F
Laboratoire de Microbiologie, Hôpital Erasme, Bruxelles, Belgium.
el Alami
S
S
Van Gossum
A
A
Struelens
M J
MJ
Serruys
E
E
D000328:Adult; D000368:Aged; D001800:Blood Specimen Collection; D002405:Catheterization, Central Venous / Q000009:adverse effects*; D002408:Catheters, Indwelling; D003937:Diagnosis, Differential; D005069:Evaluation Studies as Topic; D005334:Fever / Q000209:etiology*; D016469:Fungemia / Q000175:diagnosis; D005840:Gentian Violet; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis; D016908:Gram-Positive Bacterial Infections / Q000175:diagnosis* / Q000209:etiology / Q000382:microbiology; D006801:Humans; D008875:Middle Aged; D010289:Parenteral Nutrition, Total / Q000295:instrumentation*; D010619:Phenazines; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D018805:Sepsis / Q000175:diagnosis / Q000209:etiology / Q000382:microbiology*; D013194:Staining and Labeling; D014680:Veins
C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet