title: Enhanced MYCN expression and isochromosome 17q in pineoblastoma cell lines.
abstract: We have established two cell lines, PER-452 and PER-453, from an 8-month-old girl with an extensive pineoblastoma. Characterization of these lines revealed that the proto-oncogenes MYC and MYCN were not amplified, but both cell lines showed MYCN expression comparable to a cell line with 200-fold MYCN amplification. Both cell lines contained an i(17q). These results support the concept that pineoblastomas belong to a larger group of primitive neuroectodermal tumors of the central nervous system. These two cell lines provide a unique opportunity to investigate the molecular genetic mechanisms underlying these neoplasms further.
authors.affiliation: Division of Children's Leukemia and Cancer Research, Western Australian Research Institute for Child Health, Princess Margaret Hospital, Perth.
authors.forename: U R
authors.initials: UR
authors.lastname: Kees
authors.forename: J A
authors.initials: JA
authors.lastname: Biegel
authors.forename: J
authors.initials: J
authors.lastname: Ford
authors.forename: P R
authors.initials: PR
authors.lastname: Ranford
authors.forename: S E
authors.initials: SE
authors.lastname: Peroni
authors.forename: L A
authors.initials: LA
authors.lastname: Hallam
authors.forename: A H
authors.initials: AH
authors.lastname: Parmiter
authors.forename: M L
authors.initials: ML
authors.lastname: Willoughby
authors.forename: D
authors.initials: D
authors.lastname: Spagnolo
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002869:Chromosome Aberrations; D002886:Chromosomes, Human, Pair 17 / Q000648:ultrastructure*; D017809:Fatal Outcome; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D006801:Humans; D007223:Infant; D010870:Pineal Gland; D010871:Pinealoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured