Respiratory syncytial virus nucleocapsid protein (N) expressed in insect cells forms nucleocapsid-like structures.
The gene coding for the N protein of RSV strain Long has been cloned and sequenced. It was introduced behind the polyhedrin promoter of the shuttle vector pVL941 and baculoviruses containing the N gene were constructed by homologous recombination. Infection of Spodoptera frugiperda 9 cells resulted in the production of large amounts of a protein similar in size and antigenicity to the authentic N protein. The baculovirus expressed N protein was concentrated in the cytoplasm of the insect cells and could be extracted at low salt concentration. Nucleocapsid structures similar to those purified from RSV-infected cells could be observed by electron microscopy after negative staining of cellular extracts.
Méric
C
C
Pasteur Mérieux Sérums et Vaccins, Marcy L'Etoile, France.
Spehner
D
D
Mazarin
V
V
D000818:Animals; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D003001:Cloning, Molecular; D000939:Epitopes; D005814:Genes, Viral / Q000235:genetics*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry / Q000382:microbiology / Q000648:ultrastructure; D012136:Respiratory Syncytial Viruses / Q000737:chemistry* / Q000235:genetics; D013997:Time Factors; D014758:Viral Core Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology
D000939:Epitopes; D014758:Viral Core Proteins