Neutrophil adhesion molecules in chronic hemodialysis patients.
The expression of the adhesion molecules LFA-1 (CD11a-CD18), Mac-1 (CD11b-CD18), and LAM-1 on predialysis and intradialysis neutrophils (PMN) was analyzed by flow cytometry in 10 chronic hemodialysis patients (CHD) and compared with age-matched normal controls. All patients were dialyzed either with a polyacrylonitrile, or a polysulfone membrane. To appreciate the influence of the interdialytic time, we compared the samples of Monday (3 days without dialysis), with those of Wednesday (2 days without dialysis). A patient group not treated with recombinant human erythropoietin (rHuEPO) was also included to analyze the influence of r-HuEPO. We found on the predialysis and intradialysis samples that the expression of CD11a was not different in the CHD patients and in the normal controls. Hemodialysis was associated with a rapid and significant reduction in LAM-1 on the 15- and 30-min samples (p < 0.05). This reduction was only transient, and returned to near predialysis levels after 120 min dialysis. The MAC-1 increased significantly after 30 min dialysis (p < 0.01), and remained at the end of the dialysis procedure still substantially above the predialysis levels (p < 0.01). On the other hand, we have found a significant (p < 0.005) up-regulating of the MAC-1, and a down-regulating of the LAM-1 in the predialysis samples. We noticed further a significantly lower expression (82 +/- 9.6%) for LAM-1 in these predialysis samples (p < 0.005). These results demonstrate that 'high MAC-1, low LAM-1' neutrophils were not only a dialysis-related phenomenon, but that they were already present before the hemodialysis session, nor was there any difference between the interdialytic times or compared with the r-HuEPO treatment.
Zachée
P
P
Department of Hematology, University Hospital Gasthuisberg, Leuven, Belgium.
Daelemans
R
R
Pollaris
P
P
Boogaerts
M A
MA
Lins
R L
RL
D000328:Adult; D000368:Aged; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000097:blood*; D004921:Erythropoietin / Q000627:therapeutic use; D005260:Female; D006801:Humans; D019041:L-Selectin; D016169:Lymphocyte Function-Associated Antigen-1 / Q000097:blood; D016177:Macrophage-1 Antigen / Q000097:blood; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*; D011994:Recombinant Proteins / Q000627:therapeutic use; D006435:Renal Dialysis / Q000009:adverse effects*; D013997:Time Factors
D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D011994:Recombinant Proteins; D004921:Erythropoietin; D019041:L-Selectin