title: In vitro structural and functional relationships between preosteoclastic and bone endothelial cells: a juxtacrine model for migration and adhesion of osteoclast precursors.
abstract: The role of vascularization in the process of bone resorption has not been clarified. The interactions between vascular endothelium and osteoclast progenitors were analyzed using clonal cell lines of bone-derived endothelial and preosteoclastic cells. Insulin-like growth factor I is a major chemotactic stimulator of preosteoclastic cell migration mediated by bone endothelial cells. Osteoclast precursors rapidly adhered to bone endothelial monolayers. This phenomenon appeared to be cell-specific and mediated through the binding of vitronectin and fibronectin receptors to fibronectin. In addition, direct contact with bone endothelial cells induced osteoclast progenitors to differentiate into more mature elements, with the tendency to cluster together to form large multinucleated cells. These findings demonstrated specific in vitro interactions between bone endothelial cells and osteoclast progenitors, offering a new model for understanding the molecular mechanisms which direct the processes of osteoclast recruitment and ontogeny.
authors.affiliation: Department of Anatomy, University of Firenze, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Formigli
authors.forename: S Z
authors.initials: SZ
authors.lastname: Orlandini
authors.forename: S
authors.initials: S
authors.lastname: Benvenuti
authors.forename: L
authors.initials: L
authors.lastname: Masi
authors.forename: A
authors.initials: A
authors.lastname: Pinto
authors.forename: V
authors.initials: V
authors.lastname: Gattei
authors.forename: P A
authors.initials: PA
authors.lastname: Bernabei
authors.forename: P G
authors.initials: PG
authors.lastname: Robey
authors.forename: P
authors.initials: P
authors.lastname: Collin-Osdoby
authors.forename: M L
authors.initials: ML
authors.lastname: Brandi
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000166:cytology*; D002417:Cattle; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis; D005260:Female; D005353:Fibronectins / Q000502:physiology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D019012:Integrin beta1; D039661:Integrin beta3; D016023:Integrins / Q000096:biosynthesis / Q000502:physiology; D010010:Osteoclasts / Q000166:cytology / Q000502:physiology*; D016024:Receptors, Cytoadhesin / Q000502:physiology; D017469:Receptors, Fibronectin / Q000502:physiology; D019030:Receptors, Vitronectin
chemical_list: D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D019012:Integrin beta1; D039661:Integrin beta3; D016023:Integrins; D016024:Receptors, Cytoadhesin; D017469:Receptors, Fibronectin; D019030:Receptors, Vitronectin; D007334:Insulin-Like Growth Factor I