Combination treatments of Chinese hamster ovary cells with various restriction endonucleases result in chromosomal aberrations whose frequencies are additive or less than additive.
Chinese hamster ovary cells were treated with combinations of different restriction endonucleases (RE). The frequencies of chromosomal aberrations after combination treatments were additive or less than additive when compared with the effects of the single RE. These data indicate that DNA double-strand breaks (DSB) induced by different types of RE in combination treatments lead to chromosomal aberrations in the same way as DSB induced by single RE.
Johannes
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University GH Essen, Department of Genetics, Germany.
Obe
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D000818:Animals; D016466:CHO Cells; D002463:Cell Membrane Permeability; D002869:Chromosome Aberrations; D006224:Cricetinae; D004249:DNA Damage; D004262:DNA Restriction Enzymes / Q000633:toxicity*; D015246:Deoxyribonuclease EcoRI / Q000633:toxicity; D015252:Deoxyribonucleases, Type II Site-Specific / Q000633:toxicity; D004347:Drug Interactions; D006982:Hypertonic Solutions
D006982:Hypertonic Solutions; D004262:DNA Restriction Enzymes; D015246:Deoxyribonuclease EcoRI; C024669:endodeoxyribonuclease AluI; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C040158:GTAC-specific type II deoxyribonucleases