title: Gene for an extracellular matrix receptor protein from Pneumocystis carinii.
abstract: An initial and crucial step in the establishment of many microbial infections is the attachment of the pathogen to the host cells. Thus, adherence of Pneumocystis carinii (Pc) to type I pneumocytes is believed to be important in the induction of Pc pneumonia. Little is known about the nature of the attachment of Pc to type I cells, although extracellular matrix (ECM) proteins, such as fibronectin and laminin, have been implicated in the process. We report here the isolation of a Pc gene encoding a receptor protein that binds both fibronectin and laminin in vitro. A cDNA clone encoding the Pc ECM receptor was isolated from a Pc cDNA library and identified on the basis of sequence homology to the human colon carcinoma laminin receptor. Southern blot analysis of Pc genomic DNA confirmed that the cDNA was of Pc origin. Northern blot analysis of Pc total RNA showed a predominant mRNA of approximately 1400 nucleotides that hybridized to the ECM receptor gene. The ECM receptor predicted from the cDNA sequence is 295 amino acid residues long, with a molecular mass of 32.8 kDa. The C-terminal third of the polypeptide is highly negatively charged, whereas the N-terminal two-thirds contains hydrophobic segments that may play a role in membrane association. Sequence analysis and alignment of the N terminus with the laminin receptor cDNA sequence of human colon carcinoma support the conclusion that the Pc ECM receptor cDNA clone is a full-length clone. A Western blot of the overexpressed ECM receptor protein bound both laminin and fibronectin in vitro. Antibodies raised to the overexpressed receptor protein interacted with a 33-kDa protein in total Pc cell lysates. These findings raise the possibility that the Pc ECM receptor protein may mediate the organism's attachment to type I pneumocytes and, thus, may play a crucial role in Pc pathogenesis.
authors.affiliation: MacArthur Center for Molecular Parasitology, Yale University School of Medicine, New Haven, CT 06520.
authors.forename: S
authors.initials: S
authors.lastname: Narasimhan
authors.forename: M Y
authors.initials: MY
authors.lastname: Armstrong
authors.forename: K
authors.initials: K
authors.lastname: Rhee
authors.forename: J C
authors.initials: JC
authors.lastname: Edman
authors.forename: F F
authors.initials: FF
authors.lastname: Richards
authors.forename: E
authors.initials: E
authors.lastname: Spicer
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics*; D007797:Laminin / Q000378:metabolism; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics; D011010:Pneumocystis / Q000235:genetics*; D012313:RNA / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism*; D017474:Receptors, Laminin / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface; D017474:Receptors, Laminin; D011994:Recombinant Proteins; C052131:extracellular matrix receptor; D012313:RNA