[Distribution pattern of urothelial associated glycoproteins in human bladder cancer cell lines in in vitro culture].
The distribution of four antibodies against urothelial-associated glycoproteins (UAGAb: Uro1, -5, -9, -10, SIGNET) was studied by dual parameter flow-cytometry (UAGAb and DNA staining) in normal non-urothelial cancer cell lines (2), normal urothelial cell lines (2) and bladder cell lines (5). When cell lines were compared as plateau monolayers, high proportions of Uro1 and Uro10 were found in all cell lines, in contrast to Uro5 and Uro9, both showing specificity for urothelial cells. Though being described as a pan-urothelial marker, Uro5 is not found in all cell lines. In two cell lines (RT4 and J82) three different growth states (exponential and plateau monolayer and multicellular spheroids were compared, and dependence on spatial configuration could be shown for Uro9. Cell cycle relation of expression was found for Uro1 and Uro10.
Knuechel
R
R
Universität Regensburg.
Meckl
S
S
Masters
J R
JR
Hofstaedter
F
F
D002460:Cell Line; D004247:DNA / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis; D004848:Epithelium; D005434:Flow Cytometry; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
D004273:DNA, Neoplasm; D006023:Glycoproteins; D004247:DNA