Protective cross-reactive epitope on the nonstructural protein NS1 of influenza A virus.
We reported previously that adoptive immunization with an influenza A virus NS1-specific H-2Ld-restricted, cross-reactive, CTL clone A-11 established by stimulation with A/PR/8/34 virus (H1N1) reduced lung virus titers in mice challenged with virus in vivo (Virology 178:174-179, 1990). Using a set of recombinant vaccinia virus constructs containing truncated portions of the NS gene we have localized this cross-protective CTL epitope to the N-terminal region of the NS1 protein. This region of NS1 is active in inducing CD8+ CTL in vivo because virus-stimulated BALB/c immune spleen cells in bulk cultures also recognized the N-terminal region of the NS1 protein.
Saikh
K U
KU
Department of Medicine, University of Massachusetts Medical School, Worcester.
Tamura
M
M
Kuwano
K
K
Dai
L C
LC
West
K
K
Ennis
F A
FA
D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014407:Tumor Cells, Cultured; D017361:Viral Nonstructural Proteins / Q000276:immunology*
D000956:Antigens, Viral; D000939:Epitopes; C085820:INS1 protein, influenza virus; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins