Ion transport across the isolated intestinal mucosa of Anguilla anguilla (Pisces).
The posterior intestine of freshwater-adapted Anguilla anguilla has a serosa negative transepithelial potential difference (TPD), and a current corresponding to the flow of negative current towards serosa. The TPD and the short-circuit current (SCC) were inhibited by Na+ and K+ withdrawal, and Cl- substitution or BA2+ addition inverts TPD and SCC, suggesting a Cl- and Na+ current mucosa-serosa, a K(+)-Cl- cotransport and a K+ channel in the mucosal side. The TPD and SCC were inhibited by ouabain, DIDS, furosemide and amiloride, indicating the presence of a Na(+)-K(+)-ATPase, and Cl-/HCO3-,Na(+)-K(+)-Cl- and Na+/H+ cotransporters.
Baldisserotto
B
B
Departamento de Fisiologia, Universidade Federal de Santa Maria, RS, Brazil.
Mimura
O M
OM
D000811:Anguilla; D000818:Animals; D017609:Barium Compounds / Q000378:metabolism; D001692:Biological Transport / Q000502:physiology; D002712:Chlorides / Q000378:metabolism; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism*; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
D017609:Barium Compounds; D002712:Chlorides; D007473:Ion Channels; C024986:barium chloride; D012964:Sodium; D011188:Potassium