[Study of the transmembrane organization of the C-terminal domain of the alpha subunit of Na+,K+-ATPase using polyclonal antibodies to its perimembrane fragments].
The fragments 825-842, 868-886, 928-945, 946-967, 962-978 of the alpha-subunit of the porcine Na+,K(+)-ATPase were expressed in E. coli as fusion proteins with human tumour necrosis factor. Polyclonal antibodies were obtained to these fragments. The location of the chosen fragments according to the plasma membrane was determined with the polyclonal antibodies by ELISA on intact, lysed or solubilized pig kidney embryo cells. The fragments 825-842, 928-945, and 962-978 contained intracellular epitopes and did not contain extracellular ones; the fragments 868-886 and 946-967 did not contain any exposed epitopes.
Larin
D I
DI
Shakhparonov
M I
MI
Kostina
M B
MB
Modianov
N N
NN
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000276:immunology; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry* / Q000235:genetics / Q000276:immunology
D000906:Antibodies; D000939:Epitopes; D008565:Membrane Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D000254:Sodium-Potassium-Exchanging ATPase