title: Pharmacological effects of recombinant human granulocyte colony-stimulating factor modified by polyethylene glycol on anticancer drug-induced neutropenia in mice.
abstract: 1. To clarify the pharmacological effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) conjugated to polyethylene glycol (PEG), its effects on the number of circulating neutrophils in mice made neutropenic by cyclophosphamide (CPA) or 5-fluorouracil (5-FU) were compared with rhG-CSF lacking PEG. 2. In normal mice, PEG-conjugated rhG-CSF (PEG-rhG-CSF, 10 micrograms protein/kg) induced an increase in neutrophils which lasted for 72 h after injection whereas the effect of rhG-CSF (10 micrograms protein/kg) disappeared by 24 h after injection. 3. In CPA or 5-FU-induced neutropenic mice, PEG-rhG-CSF inhibited neutropenia or accelerated recovery from neutropenia and its potency was higher than that of rhG-CSF. 4. These results indicate that PEG-rhG-CSF has a longer duration of action than rhG-CSF and is more effective in the recovery from neutropenia.
authors.affiliation: Pharmaceutical Research Laboratory, Kirin Brewery Co. Ltd., Gunma, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: R
authors.initials: R
authors.lastname: Satake-Ishikawa
authors.forename: M
authors.initials: M
authors.lastname: Kakitani
authors.forename: M
authors.initials: M
authors.lastname: Kawagishi
authors.forename: S
authors.initials: S
authors.lastname: Matsuki
authors.forename: M
authors.initials: M
authors.lastname: Kusaka
authors.forename: K
authors.initials: K
authors.lastname: Asano
mesh_terms: D000818:Animals; D003520:Cyclophosphamide / Q000009:adverse effects* / Q000037:antagonists & inhibitors; D004353:Drug Evaluation, Preclinical; D005260:Female; D005472:Fluorouracil / Q000009:adverse effects*; D016179:Granulocyte Colony-Stimulating Factor / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008815:Mice, Inbred Strains; D009503:Neutropenia / Q000139:chemically induced / Q000188:drug therapy*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000494:pharmacology
chemical_list: D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D011092:Polyethylene Glycols; D003520:Cyclophosphamide; D005472:Fluorouracil