Molecular cloning and expression of a delta-opioid receptor from rat brain.
We have isolated and characterized a rat delta-opioid receptor. The deduced amino acid sequence (372 aa) closely resembles the murine delta-opioid receptor, DOR-1. In fact, 97% of the amino acid residues are conserved between the two species, while 93% of the nucleic acid residues are identical. A 6 kb mRNA was detected in rat cortex using rat DOR-1 as a probe. When expressed in COS cells, the clone shows high-affinity opioid binding with selectivity for delta-opioids. The rat delta-opioid receptor cDNA clone will be a useful tool for studying the function of delta-opioid receptor in rats.
Abood
M E
ME
Department of Pharmacology and Toxicology, Medical College of Virginia, Richmond.
Noel
M A
MA
Farnsworth
J S
JS
Tao
Q
Q
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001923:Brain Chemistry / Q000502:physiology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015342:DNA Probes; D018076:DNA, Complementary / Q000096:biosynthesis; D004174:Diprenorphine / Q000493:pharmacokinetics; D015723:Gene Library; D008969:Molecular Sequence Data; D009830:Olfactory Bulb / Q000378:metabolism; D012313:RNA / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017465:Receptors, Opioid, delta / Q000096:biosynthesis* / Q000235:genetics; D014162:Transfection
D015342:DNA Probes; D018076:DNA, Complementary; D017465:Receptors, Opioid, delta; D004174:Diprenorphine; D012313:RNA