A neurofilament polypeptide and the glial fibrillary acidic protein share common epitopes in the variable region.
A polyclonal antibody against the high molecular weight neurofilament polypeptide (NF-H) obtained from cytoskeletal extracts of bovine spinal cord reacted with NF-H, with the middle molecular weight neurofilament polypeptide (NF-M) and with a M(r) 51,000 polypeptide, but not with the low molecular weight neurofilament polypeptide (NF-L). The M(r) 51,000 polypeptide corresponded to the glial fibrillary acidic protein (GFAP), which forms the intermediate filaments of astrocytes. The polyclonal antibody affinity-purified with GFAP, reacted with both purified GFAP and NF-H. Digestion of NF-H with alpha-chymotrypsin was used to determine the recognition site of the affinity-purified antibodies. Only fragments of the tail domain of NF-H reacted with the antibody. We propose that common epitopes exist between the variable C-terminal domains of NF-H and GFAP.
Krauss
R Y
RY
Departamento de Biologia Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago.
Alvarez
J
J
Inestrosa
N C
NC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002918:Chymotrypsin; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis* / Q000276:immunology; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis* / Q000276:immunology; D006868:Hydrolysis; D008970:Molecular Weight; D016900:Neurofilament Proteins / Q000032:analysis* / Q000276:immunology; D013116:Spinal Cord / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005904:Glial Fibrillary Acidic Protein; D016900:Neurofilament Proteins; D002918:Chymotrypsin