Affinity gel electrophoresis of nucleic acids. Nucleobase-selective separation of DNA and RNA on agarose-poly(9-vinyladenine) conjugated gel.
Poly(9-vinyladenine) (PVAd) was immobilized within an agarose gel matrix to produce a novel affinity gel for the base-specific separation of nucleic acids by electrophoresis. The shape (single- or double-stranded) and base content of nucleic acids were specifically recognized by the affinity gel. Only single stranded DNA, of which the sequence is not regular enough to form a stable duplex hairpin structure, was selectively absorbed over double-stranded DNA. Among five polynucleotides having different bases such as poly(A), poly(G), poly(C), poly(U) and poly(I), poly(U) and poly(I) were base-specifically adsorbed by PVAd, probably by hydrogen bond formation. The effect of the molecular mass and size of poly(9-vinyladenine) was also examined.
Yashima
E
E
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Japan.
Suehiro
N
N
Miyauchi
N
N
Akashi
M
M
D000225:Adenine / Q000031:analogs & derivatives* / Q000737:chemistry; D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D011145:Polyvinyls / Q000737:chemistry*; D012313:RNA / Q000302:isolation & purification*; D012685:Sepharose / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D011145:Polyvinyls; C012002:poly(9-vinyladenine); D012313:RNA; D004247:DNA; D012685:Sepharose; D000225:Adenine