Analysis of an initial step of T cell adhesion to endothelial monolayers under flow conditions.
We analyzed an initial step of T cell adhesion to endothelial cells under flow conditions. An initial step in T cell-endothelial cell interactions was characterized either by a rolling phase, or by an immediate arrest without rolling. Anti-E- and P-selectin Abs inhibited T cell rolling on endothelial cells under flow conditions. The rolling velocity of T cells on endothelial cells increased after treatment of endothelial cells with anti-E- or P-selectin Abs. Also, rolling of T cells was observed on E-selectin-transfected CHO cells but not on ICAM-1-transfected CHO cells. Thus, rolling of T cells under flow condition was dependent on E-selectin as well as P-selectin expressed on endothelial cells. In contrast, neither anti-E- nor P-selectin Abs inhibited the immediate arrest of T cells on endothelial cells significantly and anti-ICAM-1 Abs also failed to inhibit the immediate arrest of T cells. Therefore, other adhesion molecules may play an important role in the immediate arrest of T cells under flow conditions. The number of CD45RA- T cells rolling on E-selectin-transfected CHO cells was much higher than that of CD45RA+ T cells.
Yago
T
T
Department of Immunology and Parasitology, Yokohama City University, School of Medicine, Japan.
Tsukuda
M
M
Yamazaki
H
H
Nishi
T
T
Amano
T
T
Minami
M
M
D000818:Animals; D016466:CHO Cells; D002448:Cell Adhesion / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D002478:Cells, Cultured; D006224:Cricetinae; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection; D014470:Umbilical Cord / Q000166:cytology
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D018799:Intercellular Adhesion Molecule-1; D017493:Leukocyte Common Antigens