[Immunobiologic characteristics of hybrid proteins consisting of tumor necrosis factor alpha and thymosin alpha 1].
The preparations of alpha 1-thymosin (T), alpha-tumor necrotic factor (TNF) and their based hybrid proteins: T-TNF, TNF-T, and T-TNF-T were studied by using a wide spectrum of immunobiological tests. In the L-929 cells, T-TNF preserved cytotoxicity unique to TNF; TNF-T preserved it 10 times less, and T-NTF-T was completely inactive. TNF-T inhibited the growth of Molt-4, Jm-9, Raji cells by 63 = 84%, and TNF suppressed only Raji cells by 50%. Hybrid proteins caused cell proliferation of the thymus, spleen, and lymph nodes; H-2K, CD4, and CD8 differently increased the expression of thymocytic antigens. The authors found the different effects of the drugs on phagocytosis, the production of antibody-forming cells, delayed reaction, and activation of natural killer cells. The protein T-TNF-T produced the most pronounced action.
Shmelev
V A
VA
Khromykh
L M
LM
Dudich
E I
EI
Sheviakova
L Ia
LIa
Gorbatova
E A
EA
Galaktionov
V G
VG
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects / Q000276:immunology; D014408:Biomarkers, Tumor / Q000276:immunology*; D016516:CD4-CD8 Ratio; D002455:Cell Division; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D000077596:Thymalfasin; D013947:Thymosin / Q000031:analogs & derivatives* / Q000276:immunology / Q000494:pharmacology; D013950:Thymus Gland / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology* / Q000494:pharmacology
D014408:Biomarkers, Tumor; D014409:Tumor Necrosis Factor-alpha; D013947:Thymosin; D000077596:Thymalfasin