Chemiluminescence immunosorbent assay (CLISA) and a possibility of the specific detection of soluble antigens of Clostridium botulinum type A.
A double antibody version of CLISA was demonstrated to be a rapid method (1 h) for detection and quantitative determination of Clostridium botulinum toxin antigens in biological samples. The sensitivity of this assay is about ten-fold higher than both ELISA and passive hemagglutination test. Thus, the double antibody version of CLISA appeared to be useful for the control of food products contaminated with Cl. botulinum type A bacteria.
Ligieza
J
J
Department of Microbiology, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
Reiss
J
J
Michalik
M
M
D000942:Antigens, Bacterial / Q000032:analysis*; D001904:Botulinum Antitoxin / Q000276:immunology; D001905:Botulinum Toxins / Q000032:analysis* / Q000276:immunology; D003014:Clostridium botulinum / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005504:Food Analysis / Q000379:methods; D006386:Hemagglutination Tests; D007074:Immunoglobulin G; D007163:Immunosorbent Techniques; D000922:Immunotoxins / Q000276:immunology; D008163:Luminescent Measurements; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial; D001904:Botulinum Antitoxin; D007074:Immunoglobulin G; D000922:Immunotoxins; D001905:Botulinum Toxins