Expression of Mycoplasma gallisepticum F-strain surface epitope.
The expression of a Mycoplasma gallisepticum (MG) F-strain surface epitope was studied using monoclonal antibody 6F10. Different F strain colonies were cloned based on positive or negative expression of the 6F10-recognized epitope. Clones selected for the lack of expression of this epitope (negative clones) reverted rapidly to positive expression. On the other hand, negative clones derived from F-strain-vaccinated chickens expressed the 6F10-recognized epitope variably. After in vitro passages, two groups of cultures were observed. One group reverted rapidly to positive expression. The other group exhibited a very slow rate of reversion, with 30% or fewer of their colonies expressing the 6F10-recognized epitope. These results indicate that the 6F10-recognized surface epitope of F-strain is variably expressed under in vivo and in vitro conditions.
García
M
M
Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602-4875.
Kleven
S H
SH
D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000378:metabolism*; D000954:Antigens, Surface / Q000378:metabolism; D001428:Bacterial Vaccines / Q000494:pharmacology; D002645:Chickens / Q000276:immunology* / Q000382:microbiology*; D000939:Epitopes / Q000378:metabolism; D009174:Mycoplasma / Q000145:classification / Q000254:growth & development / Q000276:immunology*; D009175:Mycoplasma Infections / Q000517:prevention & control / Q000662:veterinary; D011201:Poultry Diseases / Q000517:prevention & control
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001428:Bacterial Vaccines; D000939:Epitopes