title: Identification of seroreactive epitopes of human papillomavirus type 18 E7 protein by synthetic peptides.
abstract: Nine everlapping peptides covering the entire sequence of early protein E7 of human papillomavirus type 18 (HPV-18) were synthesized and tested as antigens with pools of selected human sera in ELISA. Peptides denoted 18/E7-2, 18/E7-3, and 18/E7-5 (amino acid positions 11-33, 21-40, and 41-60, respectively) were reactive with pooled sera originating from HPV-18 DNA-positive cervical cancer patients but not with sera from HPV-16 DNA-positive cervical cancer patients or from condyloma acuminata patients. This suggested that the epitopes contained in these peptides were HPV-18 type-specific, relative to HPV types 16, 11, and 6. On the other hand, 18/E7-1 (aa 1-23) and 18/E7-6 (aa 51-70) peptides were cross-reactive. The prevalence of antibodies reactive with 18/E7-2, 18/E7-3, and 18/E7-5 peptides in cervical carcinoma patients was very low. Thus, the utilization of these peptides for monitoring HPV-18 infection seems to be rather limited.
authors.affiliation: Research Institute for Feed Supplements and Veterinary Drugs, Jílové near Prague, Czech Republic.
authors.forename: V
authors.initials: V
authors.lastname: Krchnák
authors.forename: T
authors.initials: T
authors.lastname: Pistĕk
authors.forename: J
authors.initials: J
authors.lastname: Vágner
authors.forename: A
authors.initials: A
authors.lastname: Suchánková
authors.forename: J
authors.initials: J
authors.lastname: Kañka
authors.forename: L
authors.initials: L
authors.lastname: Ritterová
authors.forename: V
authors.initials: V
authors.lastname: Vonka
mesh_terms: D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics*; D003218:Condylomata Acuminata / Q000276:immunology / Q000382:microbiology; D004268:DNA-Binding Proteins; D000939:Epitopes / Q000235:genetics; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000276:immunology*; D027383:Papillomaviridae / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000276:immunology / Q000382:microbiology; D010455:Peptides / Q000138:chemical synthesis / Q000235:genetics* / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D014412:Tumor Virus Infections / Q000276:immunology / Q000382:microbiology; D002583:Uterine Cervical Neoplasms / Q000276:immunology / Q000382:microbiology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D004268:DNA-Binding Proteins; C052633:E7 protein, Human papillomavirus type 18; D000939:Epitopes; D009856:Oncogene Proteins, Viral; D010455:Peptides