RNA/DNA hybrid duplexes with identical nearest-neighbor base-pairs have identical stability.
Energetic behaviors of eight pairs of RNA/DNA hybrid duplexes with identical nearest neighbors have been investigated by UV melting analysis. In the pairs with identical nearest-neighbor pairs, the melting curve traces at the same strand concentration were very similar. The average difference in stabilization energy of these pairs was 4%, which was about expected within experimental error. These results indicate that the nearest-neighbor model is valid for predicting the stability of RNA/DNA hybrid duplexes as well as RNA/RNA and DNA/DNA duplexes.
Sugimoto
N
N
Department of Chemistry, Faculty of Science, Konan University, Kobe, Japan.
Katoh
M
M
Nakano
S
S
Ohmichi
T
T
Sasaki
M
M
D001482:Base Composition / Q000235:genetics*; D001483:Base Sequence; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry* / Q000235:genetics; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D009843:Oligoribonucleotides / Q000138:chemical synthesis; D012313:RNA / Q000737:chemistry* / Q000235:genetics; D013816:Thermodynamics
D009692:Nucleic Acid Heteroduplexes; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D012313:RNA; D004247:DNA