Clinical application of reverse transcription-polymerase chain reaction for HIV infection.
This article describes the current knowledge of the various RT-PCR assays and their clinical application in HIV disease. The importance of assay precision and sample handling is emphasized. A review of quantification techniques is also presented. Finally, the relevant literature describing the application of RT-PCR for both virion RNA in plasma and cellular mRNA is reviewed.
Holodniy
M
M
Division of Infectious Diseases, Department of Veterans Affairs Medical Center, Palo Alto, California.
D006678:HIV / Q000235:genetics; D015658:HIV Infections / Q000175:diagnosis / Q000821:virology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D012367:RNA, Viral / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid; D012194:RNA-Directed DNA Polymerase; D012680:Sensitivity and Specificity
D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase