[Analysis of effectiveness of cDNA synthesis, induced using complementary primers and primers containing a noncomplementary base matrix].
We have studied the efficiency of DNA synthesis catalyzed by M-MLV reverse transcriptase or Thermus aquaticus DNA polymerase for primers (4-17 nucleotides long) either completely matched or possessing a single mismatched base pair at all possible positions in the primer. It has been shown that DNA synthesis efficiency depends not only on the position of mismatched base pair but on the length and primary structure of the primer. The enzyme, template, and primer concentrations determine the relative level of mismatched DNA synthesis.
D'iachenko
L B
LB
Chenchik
A A
AA
Khaspekov
G L
GL
Tatarenko
A O
AO
Bibilashvili
R Sh
RSh
D001482:Base Composition; D001483:Base Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000138:chemical synthesis*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D008324:Mammary Tumor Virus, Mouse / Q000201:enzymology; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D019914:Taq Polymerase; D013698:Templates, Genetic
D017931:DNA Primers; D018076:DNA, Complementary; D019914:Taq Polymerase; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase