Purified protein products of rDNA technology expressed in animal cell culture.
During the past 7 years, 14 versions of 7 rDNA proteins have been licensed which are derived from animal cell culture expression systems. These medically useful products have included hormones, coagulation factors, enzymes and a vaccine. Aspects of the molecular complexity, manufacture, control and utilization of these products are discussed. In contrast to previous generations of biological production technology, the technology for production of rDNA-derived proteins in animal cells appears to be safe.
Lubiniecki
A S
AS
SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406.
Lupker
J H
JH
D000818:Animals; D001688:Biological Products / Q000235:genetics / Q000302:isolation & purification*; D001709:Biotechnology; D002478:Cells, Cultured; D004274:DNA, Recombinant / Q000235:genetics*; D003850:Deoxyribonuclease I / Q000235:genetics / Q000302:isolation & purification; D004921:Erythropoietin / Q000235:genetics / Q000302:isolation & purification; D005169:Factor VIII / Q000235:genetics / Q000302:isolation & purification; D016179:Granulocyte Colony-Stimulating Factor / Q000235:genetics / Q000302:isolation & purification; D013006:Growth Hormone / Q000235:genetics / Q000302:isolation & purification; D017325:Hepatitis B Vaccines / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification*; D012449:Safety; D010959:Tissue Plasminogen Activator / Q000235:genetics / Q000302:isolation & purification; D014614:Vaccines, Synthetic / Q000235:genetics / Q000302:isolation & purification
D001688:Biological Products; D004274:DNA, Recombinant; D017325:Hepatitis B Vaccines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor; D005169:Factor VIII; D013006:Growth Hormone; D003850:Deoxyribonuclease I; D010959:Tissue Plasminogen Activator