title: Circulating soluble adhesion molecules in systemic vasculitis.
abstract: The plasma levels of soluble intercellular adhesion molecule-1 (sICAM-1), E-selectin (sE-selectin), and vascular cell adhesion molecule-1 (sVCAM-1), might reflect endothelial activation and injury and would therefore be useful markers of disease activity in vasculitis. To investigate this we measured the levels of sICAM-1, sE-selectin, and sVCAM-1 by two-site ELISAs in the plasma of patients with (a) active vasculitis (n = 16), (b) vasculitis in remission (n = 15), (c) chronic renal failure (CRF) (n = 10), and (d) normal healthy controls (n = 10). Plasma sICAM-1 levels were significantly higher in patients with active vasculitis, 323 ng/ml (193-607) compared with patients with inactive vasculitis, 199 ng/ml (131-297); P = 0.0006 and healthy controls, 188 ng/ml (138-259); P = 0.0002. Plasma sE-selectin levels were also significantly higher in the patients with active vasculitis, 45 ng/ml (15-65) compared with patients with inactive vasculitis, 25 ng/ml (15-55); P = 0.027 but not when compared with healthy controls, 35 ng/ml (20-55); P = 0.16. There was no difference in plasma sVCAM-1 levels between patients with active vasculitis, OD 0.56 (0.45-0.85) and inactive disease, OD 0.58 (0.47-0.79) (P = 0.12) or with healthy controls OD 0.49 (0.42-0.68) (P = 0.48). There were no significant differences between the plasma levels of any of the soluble adhesion molecules between patients with active vasculitis and patients with chronic failure.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Renal Research Laboratories, Queen Elizabeth Hospital, Edgbaston, Birmingham, University of Birmingham, Birmingham, UK.
authors.forename: A A
authors.initials: AA
authors.lastname: Pall
authors.forename: D
authors.initials: D
authors.lastname: Adu
authors.forename: M
authors.initials: M
authors.lastname: Drayson
authors.forename: C M
authors.initials: CM
authors.lastname: Taylor
authors.forename: N T
authors.initials: NT
authors.lastname: Richards
authors.forename: J
authors.initials: J
authors.lastname: Michael
mesh_terms: D002097:C-Reactive Protein / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000097:blood*; D003404:Creatinine / Q000097:blood; D019040:E-Selectin; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood*; D007676:Kidney Failure, Chronic / Q000097:blood; D007958:Leukocyte Count; D009391:Nephelometry and Turbidimetry; D012217:Rheumatoid Factor / Q000032:analysis; D012995:Solubility; D019010:Vascular Cell Adhesion Molecule-1; D014657:Vasculitis / Q000097:blood*; D014841:von Willebrand Factor / Q000032:analysis
chemical_list: D015815:Cell Adhesion Molecules; D019040:E-Selectin; D019010:Vascular Cell Adhesion Molecule-1; D014841:von Willebrand Factor; D018799:Intercellular Adhesion Molecule-1; D002097:C-Reactive Protein; D012217:Rheumatoid Factor; D003404:Creatinine