Electroporation and streptolysin O--a comparison of poration techniques.
CHO (Chinese hamster ovary), xrs5 (X-ray sensitive Chinese hamster) and HF19 (untransformed human fibroblast) cells, were exposed to a lethal dose of the restriction enzyme Pvu II during electroporation or poration with the bacterial toxin streptolysin O. The uptake of the exclusion dye trypan blue was used as a measure of poration and compared with survival as measured by subsequent colony formation. It was assumed that any surviving cells had not been permeabilized and therefore did not receive any restriction enzyme. Electroporation alone proved to be more cytotoxic to the cells, whilst streptolysin O was more efficient at permeabilizing both hamster and human cells.
Harvey
A N
AN
MRC Radiobiology Unit, Chilton, Didcot, UK.
Costa
N D
ND
Savage
J R
JR
D000818:Animals; D001426:Bacterial Proteins; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D015252:Deoxyribonucleases, Type II Site-Specific; D018274:Electroporation / Q000379:methods*; D006801:Humans; D013301:Streptolysins / Q000737:chemistry*
D001426:Bacterial Proteins; D013301:Streptolysins; C025964:streptolysin O; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific