Fluorescent differential display: arbitrarily primed RT-PCR fingerprinting on an automated DNA sequencer.
We established robust, reliable protocols for 'Differential Display (DD),' an RNA fingerprinting method originally developed by Liang and Pardee [(1992) Science 257, 967-971] using RT-PCR with arbitrary primers. Our protocols are optimized so that reliable DD analysis can be performed on a fluorescent DNA sequencer to ensure high throughput as well as improved operational safety, compared with the original one using radioactive compounds. Such 'Fluorescent Differential Display (FDD)' techniques will accelerate the identification of differentially expressed as well as polymorphic transcripts to address various biological questions.
Ito
T
T
Human Genome Center, Institute of Medical Science, University of Tokyo, Japan.
Kito
K
K
Adati
N
N
Mitsui
Y
Y
Hagiwara
H
H
Sakaki
Y
Y
D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers; D005453:Fluorescence; D005821:Genetic Techniques / Q000295:instrumentation*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012313:RNA / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000295:instrumentation*; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D012333:RNA, Messenger; D012313:RNA