Binding and specificity of major immunoglobulin classes of preformed human anti-pig heart antibodies.
Preformed human anti-pig antibodies isolated from perfused pig hearts were used to analyze the binding of various immunoglobulin classes to cultured pig kidney cells. All anti-pig immunoglobulins (i.e., IgG, IgA, and IgM) were localized on the cell surface by the use of an indirect immunofluorescence technique. Anti-pig immunoglobulins also competed for the pig cell surface epitopes with Griffonia simplicifolia lectin (GS-I-B4), which is specific for alpha-galactosyl residues. This study provides further evidence that preformed human antibodies recognizing alpha-galactosyl-containing epitopes (anti-gal antibodies) could be an important factor in hyperacute rejection of pig organs.
Koren
E
E
Oklahoma Medical Research Foundation, Oklahoma City 73104.
Neethling
F A
FA
Richards
S
S
Koscec
M
M
Ye
Y
Y
Zuhdi
N
N
Cooper
D K
DK
D000818:Animals; D000918:Antibody Specificity; D001667:Binding, Competitive; D002478:Cells, Cultured; D000939:Epitopes; D005690:Galactose; D006801:Humans; D007136:Immunoglobulins / Q000145:classification / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D037102:Lectins; D009206:Myocardium / Q000276:immunology*; D013552:Swine
D000939:Epitopes; D007136:Immunoglobulins; D037102:Lectins; D005690:Galactose