Differential induction of adult and fetal globin gene expression in the human CML cell subline KU-812F/33.
Various chemicals which are known to have positive effects on differentiation of some erythroid cell lines were tested on a human chronic myelogenous leukemia cell line, KU-812F. Succinic acid, 5-azacytidine, daunomycin, and hemin showed a positive effect. Among them, hemin and 5-azacytidine were the most effective inducers for erythroid differentiation of KU-812F cells. Dimethylsulfoxide, cytosine arabinofuranoside, and sodium n-butyrate showed no effect. In addition, subclone KU-812F/33 derived from the KU-812F cell line showed differential expression of the beta- and gamma-globin genes in the presence of either 2 microM 5-azacytidine or 40 microM hemin. Hemoglobin synthesis in differentiated KU-812F/33 cells was analyzed by isoelectric focusing gel electrophoresis, and S1 mapping analysis of beta- and gamma-globin mRNA was performed. After treatment with 5-azacytidine, the beta-globin gene expression was predominantly enhanced (18.75-fold higher level of beta-globin mRNA). After treatment with hemin, the most notable increase was in the gamma-globin gene expression (1.83-fold higher level of gamma-globin mRNA), while no increment of beta-globin was observed.
Endo
T
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Department of School-Nurse Training, Kyushu Women's Jr. College, Fukuoka.
Ishibashi
Y
Y
Shiokawa
S
S
Fukumaki
Y
Y
Okano
H
H
D001374:Azacitidine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D003630:Daunorubicin / Q000494:pharmacology; D004357:Drug Synergism; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000166:cytology* / Q000187:drug effects; D005319:Fetal Hemoglobin / Q000096:biosynthesis; D005914:Globins / Q000096:biosynthesis / Q000235:genetics*; D006427:Hemin / Q000494:pharmacology; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D007525:Isoelectric Focusing; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive; D013386:Succinates / Q000494:pharmacology; D019802:Succinic Acid; D014407:Tumor Cells, Cultured
D006454:Hemoglobins; D013386:Succinates; D006427:Hemin; D005914:Globins; D005319:Fetal Hemoglobin; D019802:Succinic Acid; D001374:Azacitidine; D003630:Daunorubicin