title: Changes in annexin I and II levels during the postnatal development of rat pancreatic islets.
abstract: The expression patterns and the dynamic changes in content of both annexin I and annexin II in the rat pancreatic islets during postnatal development were investigated by both western blot analysis and immunohistochemistry. Immunohistochemical methods clearly demonstrated the presence of annexins I and II exclusively in pancreatic islets, while exocrine tissues were not stained by anti-annexin antibodies. Pancreatic islets were diffusely stained with no specific differences in distribution between different cell types. The expression of annexin I in pancreatic islets gradually increased with postnatal development. A developmental study of annexins I and II by western blot analysis essentially supported the results obtained by immunohistochemistry. In addition, the increasing expression of two protein tyrosine kinases, epidermal growth factor-receptor/kinase and pp60src, which phosphorylate annexin I and annexin II, respectively, and of protein kinase C, which phosphorylates both proteins, was also shown during postnatal development in rat pancreatic islets. Thus, a relationship between the expression of annexins I and II and the maturation of islet cell function is suggested.
authors.affiliation: Department of Physiology, Kagawa Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohnishi
authors.forename: M
authors.initials: M
authors.lastname: Tokuda
authors.forename: T
authors.initials: T
authors.lastname: Masaki
authors.forename: T
authors.initials: T
authors.lastname: Fujimura
authors.forename: Y
authors.initials: Y
authors.lastname: Tai
authors.forename: H
authors.initials: H
authors.lastname: Matsui
authors.forename: T
authors.initials: T
authors.lastname: Itano
authors.forename: T
authors.initials: T
authors.lastname: Ishida
authors.forename: J
authors.initials: J
authors.lastname: Takahara
authors.forename: R
authors.initials: R
authors.lastname: Konishi
mesh_terms: D000199:Actins / Q000302:isolation & purification; D000818:Animals; D017305:Annexin A1 / Q000302:isolation & purification*; D017306:Annexin A2 / Q000302:isolation & purification*; D015153:Blotting, Western; D002469:Cell Separation; D066246:ErbB Receptors; D005934:Glucagon / Q000302:isolation & purification; D007150:Immunohistochemistry; D007328:Insulin / Q000302:isolation & purification; D007515:Islets of Langerhans / Q000737:chemistry* / Q000254:growth & development*; D008297:Male; D011494:Protein Kinases; D016392:Proto-Oncogene Proteins pp60(c-src); D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000199:Actins; D017305:Annexin A1; D017306:Annexin A2; D007328:Insulin; D005934:Glucagon; D011494:Protein Kinases; D066246:ErbB Receptors; D016392:Proto-Oncogene Proteins pp60(c-src)