Effect of toluene inhalation on extracellular striatal acetylcholine release studied with microdialysis.
Intracerebral microdialysis was performed on awake, freely moving rats in order to record effect of toluene exposure on acetylcholine release in striatum. Acetylcholine release decreased during (about 20%) and after (about 60%) toluene exposure (2 hr, 2000 p.p.m.) Striatal acetylcholine release is thought to be mediated by dopamine. In a previous work we found that extracellular dopamine levels increase during toluene exposure. A dopamine uptake inhibitor (LU 19-005, 2 mg/kg) was therefore injected subcutaneously and the effect of increased extracellular dopamine on acetylcholine release within the striatum was monitored in the absence of toluene exposure. LU 19-005 increased striatal dopamine levels six times and the acetylcholine levels increased to about 145% of basal value. The present study shows that toluene exposure decrease acetylcholine release while an injection of a dopamine uptake inhibitor fails to decrease acetylcholine release. Indicating that acute exposure of toluene decreases striatal acetylcholine release by a mechanism that is not mediated by increased extracellular dopamine levels. Our data suggest that toluene decrease acetylcholine release within the striatum and that this effect not is mediated by increased extracellular dopamine levels.
Stengård
K
K
Department of Physiology and Pharmacology, Karolinska Institute, Stockholm, Sweden.
D000109:Acetylcholine / Q000378:metabolism*; D000280:Administration, Inhalation; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology; D007189:Indans / Q000494:pharmacology; D008297:Male; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014050:Toluene / Q000008:administration & dosage / Q000633:toxicity*
D018765:Dopamine Uptake Inhibitors; D007189:Indans; D014050:Toluene; C045117:Lu 19005; D000109:Acetylcholine; D004298:Dopamine