Increased tyrosine-phosphorylation of 55KDa proteins in beta-actin/Tec transgenic mice.
Protein-tyrosine kinases are considered to play important roles in cell proliferation and differentiation. Tec is a cytoplasmic protein-tyrosine kinase expressed in liver and hematopoietic tissues. To better understand Tec function in vivo, we generated transgenic mice expressing tec driven by the cytomegarovirus enhancer and beta-actin promoter. Among six transgenic lines generated, a particular line, named 2-11, expressed tec transgene product more widely and abundantly than the other lines. In the tissues of 2-11, the kinase activity of Tec was enhanced in accordance with the high expression of tec transgene product. Interestingly, tyrosine-phosphorylation of approximately 55KDa proteins in the tissues was induced. These results suggest that cellular proteins of 55KDa might be potential substrates of Tec in vivo.
Honda
H
H
Department of Molecular Biology, Jichi Medical School, Tochigi-ken, Japan.
Mano
H
H
Katsuki
M
M
Yazaki
Y
Y
Hirai
H
H
D000199:Actins / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D015153:Blotting, Western; D002645:Chickens; D003587:Cytomegalovirus; D004742:Enhancer Elements, Genetic; D015870:Gene Expression; D005914:Globins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008970:Molecular Weight; D010766:Phosphorylation; D019000:Phosphotyrosine; D011401:Promoter Regions, Genetic; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D011817:Rabbits; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism
D000199:Actins; D019000:Phosphotyrosine; D014443:Tyrosine; D005914:Globins; C089848:Tec protein-tyrosine kinase; D011505:Protein-Tyrosine Kinases