Dexamethasone inhibits the expression of an inducible nitric oxide synthase in infarcted rabbit myocardium.
Infarcted areas of rabbit myocardium show relatively higher inducible nitric oxide synthase activity, measured by the conversion of L-[14C]arginine to L-[14C]citrulline. The principal finding in this study is that dexamethasone (2 mg/kg) prevents the induction of inducible nitric oxide synthase in heart muscle when given before, or even 3 hr after coronary artery ligation. Additionally cyclic GMP levels remain unchanged following treatment with dexamethasone. It is possible that the enhanced production of nitric oxide by inducible nitric oxide synthase accounts, at least in part, for the depression of myocardial contractility seen in myocardial infarction and in other clinical conditions.
Dudek
R R
RR
Department of Experimental Cardiology, Huntington Medical Research Institutes, Pasadena, California 91101.
Wildhirt
S
S
Pinto
V
V
Giesler
G
G
Bing
R J
RJ
D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000097:blood; D000818:Animals; D001120:Arginine / Q000378:metabolism; D002956:Citrulline / Q000378:metabolism; D003331:Coronary Vessels / Q000601:surgery; D006152:Cyclic GMP / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D008026:Ligation; D008297:Male; D009203:Myocardial Infarction / Q000201:enzymology* / Q000209:etiology; D009206:Myocardium / Q000201:enzymology; D019001:Nitric Oxide Synthase; D011817:Rabbits
D002956:Citrulline; D003907:Dexamethasone; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D006152:Cyclic GMP