The production of granulocyte colony-stimulating factor and interleukin 6 by human bone marrow stromal cells in aplastic anemia.
Stromal cells are known to produce granulocyte colony-stimulating factor (G-CSF) and interleukin 6 (IL-6). To assess the function of bone marrow stromal cells in the pathological state, we studied their in vitro ability to produce G-CSF and IL-6 in 9 patients with aplastic anemia and 5 normal volunteers, using an enzyme immunoassay method. Constitutive production of IL-6, but not G-CSF, was detected in almost all cases. Interestingly, the inducible production of G-CSF and IL-6 by stromal cells after stimulation with interleukin-1 and/or lipopolysaccharides was severely depressed in 3 patients with aplastic anemia. Our results may reflect poor hematopoietic activity in the bone marrow in some cases of aplastic anemia.
Tani
K
K
Department of Hematology/Oncology, University of Tokyo, Japan.
Ozawa
K
K
Ogura
H
H
Takahashi
S
S
Takahashi
K
K
Tsuruta
T
T
Okano
A
A
Akiyama
Y
Y
Yoshikubo
T
T
Shimane
M
M
D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000378:metabolism* / Q000473:pathology; D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D001854:Bone Marrow Cells; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D007085:Ilium / Q000378:metabolism; D007124:Immunoenzyme Techniques; D015850:Interleukin-6 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D017154:Stromal Cells / Q000378:metabolism*
D015850:Interleukin-6; D008070:Lipopolysaccharides; D016188:Receptors, Granulocyte Colony-Stimulating Factor; D016179:Granulocyte Colony-Stimulating Factor