Expression of human endogenous retrovirus (HERV-K) in chronic myeloid leukemia.
We have previously demonstrated the presence of a reverse transcriptase-like enzyme in retroviral particles from patients with essential thrombocythemia, polycythemia vera, and chronic myelogenous leukemia. It was subsequently shown that the human genome contains 50 copies of HERV-K. HERV-K is a human endogenous class I retroviral element that contains gag, pol, and env open reading frames. Using both reverse transcriptase-polymerase chain reaction and ribonuclease protection assays, it is demonstrated that the HERV-K pol is expressed in human blood leukocytes. The data indicates that this expression is restricted in CML white cells and is the result of gene regulation.
Brodsky
I
I
Department of Neoplastic Diseases, Hahnemann University Hospital, Philadelphia, Pennsylvania 19102.
Foley
B
B
Gillespie
D
D
D000595:Amino Acid Sequence; D015415:Biomarkers / Q000032:analysis; D016026:Bone Marrow Transplantation; D015973:Gene Expression Regulation, Leukemic; D015967:Gene Expression Regulation, Viral; D015751:Genes, pol; D016679:Genome, Viral; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000382:microbiology*; D007953:Leukemia, Radiation-Induced / Q000453:epidemiology; D007962:Leukocytes / Q000382:microbiology; D008969:Molecular Sequence Data; D009857:Oncogenes; D011087:Polycythemia Vera / Q000382:microbiology; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase / Q000032:analysis / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D012190:Retroviridae / Q000235:genetics / Q000302:isolation & purification*; D012191:Retroviridae Proteins / Q000032:analysis / Q000235:genetics
D015415:Biomarkers; D012191:Retroviridae Proteins; D012194:RNA-Directed DNA Polymerase