title: Entrance and survival of Salmonella typhimurium and Yersinia enterocolitica within human B- and T-cell lines.
abstract: Lymphocytes, located within the Peyer's patches, might be involved in the dissemination of enteropathogenic Salmonella typhimurium and Yersinia enterocolitica bacteria. To test this hypothesis, we have investigated the susceptibility of human B- and T-cell lines to bacterial adhesion and invasion. The two S. typhimurium strains analyzed were highly invasive, while the two Y. enterocolitica (O:8) strains adhered to the B- and T-cell lines but did not enter the cell lines in significant amounts. We hypothesize that the incapability of the Y. enterocolitica (O:8) strains to enter the human B- and T-cell lines is most probably due to the bacterial inability to induce the internalization process upon adhesion to both cell lines. Although immortalized B- and T-cell lines were used in this study, the results presented suggest the possibility that both cell types could play a role in the dissemination of intracellularly residing S. typhimurium in vivo.
authors.affiliation: Department of Ophthalmo-Immunology, The Netherlands Ophthalmic Research Institute, Amsterdam.
authors.forename: G M
authors.initials: GM
authors.lastname: Verjans
authors.forename: J H
authors.initials: JH
authors.lastname: Ringrose
authors.forename: L
authors.initials: L
authors.lastname: van Alphen
authors.forename: T E
authors.initials: TE
authors.lastname: Feltkamp
authors.forename: J G
authors.initials: JG
authors.lastname: Kusters
mesh_terms: D001402:B-Lymphocytes / Q000382:microbiology*; D001422:Bacterial Adhesion; D002460:Cell Line; D006801:Humans; D019012:Integrin beta1; D016023:Integrins / Q000032:analysis; D012486:Salmonella typhimurium / Q000254:growth & development*; D013601:T-Lymphocytes / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000254:growth & development*
chemical_list: D019012:Integrin beta1; D016023:Integrins