Sequence and expression of a baculovirus protein with antigenic similarity to telokin.
A protein from baculovirus-infected cells reacted with an antibody against the smooth muscle protein telokin. Because of this unusual similarity, the protein, termed telokin-like protein-20 (TLP20), was isolated and characterized. Its M(r) on denaturing polyacrylamide gels was 28K and the protein contained a high proportion of beta structure. A cDNA for TLP20 was isolated and sequenced. The 3' non-coding sequence contained a region of high identity with the 5' end of two other baculovirus genes. The 5' non-coding region contains several baculovirus regulatory elements. Surprisingly, the derived amino acid sequence showed no homologies to telokin. The cDNA was cloned into a bacterial expression vector and the subsequently expressed protein had a slightly lower M(r) than the native protein, but cross-reacted with telokin antibody. This paper reports the characterization of a new baculovirus protein that shares some antigenic similarities to the smooth muscle protein telokin.
Raynes
D A
DA
Department of Animal Sciences, University of Arizona, Tucson 85721.
Hartshorne
D J
DJ
Guerriero
V
V
D000595:Amino Acid Sequence; D000906:Antibodies; D001483:Base Sequence; D003429:Cross Reactions; D018076:DNA, Complementary; D000939:Epitopes / Q000032:analysis*; D005814:Genes, Viral / Q000235:genetics*; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D009219:Myosin-Light-Chain Kinase; D017924:Nucleopolyhedroviruses / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames; D010446:Peptide Fragments; D010455:Peptides; D017433:Protein Structure, Secondary; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics*
C088126:Ac-TLP protein, Autographa californica nucleopolyhedrovirus; D000906:Antibodies; D018076:DNA, Complementary; D000939:Epitopes; D009124:Muscle Proteins; D010446:Peptide Fragments; D010455:Peptides; D014764:Viral Proteins; D015678:Viral Structural Proteins; C060640:telokin; D009219:Myosin-Light-Chain Kinase