title: Comparison of three immunoassays for the determination of N-acetylprocainamide. Determination of false elevations by a polyclonal antibody-based method.
abstract: The performance of the ROCHE FP NAPA reagent, a newly reformulated monoclonal antibody-based fluorescence polarization immunoassay (FPIA) for N-acetylprocainamide, was compared with two commercially available polyclonal antibody-based immunoassays, the Abbott TDx and Syva EMIT. Although excellent correlation was observed between the ROCHE FP NAPA reagent and the Syva EMIT reagent, a positive bias was observed in the correlation between the ROCHE FP NAPA reagent and the Abbott TDx reagent. The bias in the TDx method was investigated further by the concurrent high-performance liquid chromatography (HPLC) analysis of patient samples. Insignificant differences (p < 0.05) were observed between the HPLC and ROCHE FP values. The TDx system, however, yielded significantly higher (p < 0.0005) results than did HPLC.
authors.affiliation: Department of Pathology, Brown University, Providence, Rhode Island.
authors.forename: J P
authors.initials: JP
authors.lastname: Laurino
authors.forename: J
authors.initials: J
authors.lastname: McKay
authors.forename: E
authors.initials: E
authors.lastname: Fischberg
authors.forename: K
authors.initials: K
authors.lastname: Schwenzer
authors.forename: R
authors.initials: R
authors.lastname: Kaufman
mesh_terms: D000120:Acecainide / Q000097:blood* / Q000276:immunology; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid; D016231:Fluorescence Polarization Immunoassay; D005615:Freezing; D006801:Humans; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D000120:Acecainide