Production and selection of mutants of Leuconostoc mesenteroides constitutive for glucansucrases.
After chemical mutagenesis using ethyl methane sulfonate, we isolated mutants constitutive for glucansucrases from Leuconostoc mesenteroides NRRL B-512FM, B-1142, and B-1355. Those mutants produced glucansucrases when grown on D-glucose as well as on sucrose. They produced higher glucansucrase activities (3 to 22 times) when grown on D-glucose than the parent strains grown on sucrose. Glucansucrases from mutants B-1355C and B-1142C grown on glucose formed glucans that were highly resistant to Penicillium dextranase hydrolysis. Mutant B-512FMC dextransucrase formed the same kind of dextran as the parent strain; however, it showed higher thermal stability, even when dextran was absent.
Kim
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Department of Biochemistry and Biophysics, Iowa State University, Ames 50011.
Robyt
J F
JF
D001709:Biotechnology; D003911:Dextrans / Q000096:biosynthesis; D004795:Enzyme Stability; D005936:Glucans / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D016695:Glycosyltransferases; D006863:Hydrogen-Ion Concentration; D007934:Leuconostoc / Q000201:enzymology* / Q000235:genetics* / Q000254:growth & development; D009154:Mutation; D013395:Sucrose / Q000378:metabolism; D013696:Temperature
D003911:Dextrans; D005936:Glucans; D013395:Sucrose; D016695:Glycosyltransferases; C033837:alternansucrase; D006026:Glycoside Hydrolases; D005947:Glucose