[Releases of bradykinin and substance P by heating hind paw of rat].
Contribution of kallikrein-kinin system to heat-induced substance P (SP) release into the periphery was studied by using plasma kininogens-deficient strain Brown Norway Katholiek (B/N-Ka) and normal strain Brown Norway Kitasato (B/N-Ki) rats. Bradykinin (BK) and SP levels in the sc perfusates of the hind instep were measured by radioimmunoassay. In B/N-Ki rat, immersion of hind paw into hot water (47 degrees C) for 20 min led to an increase of BK (43 +/- s 34 fmol.min-1) and SP (11.1 +/- 9.7 fmol.min-1) in the perfusate, whereas those in B/N-Ka rat (BK 1.3 +/- 1.0 fmol.min-1 (P < 0.01), SP 5.5 +/- 3.5 fmol.min-1 (P < 0.05)) were remarkably less. Heat-induced extravasation (leakage of Evans blue) in B/N-Ka rat was also less than that in B/N-Ki rat (P < 0.05). Results suggest that kallikrein-kinin system is involved in the release of SP into the periphery, ie, BK released into the extravascular space by noxious heat stimulation intervenes in SP release.
Tang
F D
FD
Department of Pharmacology, Faculty of Dentistry, Osaka University, Japan.
Yonehara
N
N
Imai
Y
Y
Takiuchi
S
S
Inoki
R
R
Bian
R L
RL
D000818:Animals; D001920:Bradykinin / Q000378:metabolism*; D003677:Deficiency Diseases / Q000378:metabolism; D005260:Female; D006358:Hot Temperature; D007704:Kininogens / Q000172:deficiency*; D008297:Male; D010477:Perfusion; D051381:Rats; D011914:Rats, Inbred BN; D013373:Substance P / Q000378:metabolism*
D007704:Kininogens; D013373:Substance P; D001920:Bradykinin