title: Fibroblast growth factor-1 induces phosphofructokinase, fatty acid synthase and Ca(2+)-ATPase mRNA expression in NIH 3T3 cells.
abstract: Polypeptide growth factors act in part by inducing the expression of specific proteins that perform functions critical to cell cycle progression. We have used a differential display technique to identify genes that are expressed at higher levels following fibroblast growth factor (FGF)-1 (acidic FGF) stimulation of quiescent murine NIH 3T3 fibroblasts. Three such genes--liver (B-type) phosphofructokinase (PFK), fatty acid synthase (FAS) and sarco(endo)plasmic reticulum Ca(2+)-ATPase type 2 (SERCA2)--are described in this report. The level of FAS and SERCA2 mRNA expression is increased rapidly after FGF-1 addition; in contrast, PFK mRNA is induced with kinetics more typical of delayed-early genes. These results indicate that enhanced expression of the PFK, FAS and SERCA2 proteins may be important for FGF-1-stimulated cell proliferation.
authors.affiliation: Department of Molecular Biology, Holland Laboratory, American Red Cross, Rockville, Maryland 20855.
authors.forename: D K
authors.initials: DK
authors.lastname: Hsu
authors.forename: P J
authors.initials: PJ
authors.lastname: Donohue
authors.forename: G F
authors.initials: GF
authors.lastname: Alberts
authors.forename: J A
authors.initials: JA
authors.lastname: Winkles
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D000252:Calcium-Transporting ATPases / Q000096:biosynthesis*; D017931:DNA Primers; D004790:Enzyme Induction / Q000187:drug effects; D064429:Fatty Acid Synthases / Q000096:biosynthesis*; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D051379:Mice; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D010732:Phosphofructokinase-1 / Q000096:biosynthesis*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D016220:Fibroblast Growth Factor 1; D064429:Fatty Acid Synthases; D010732:Phosphofructokinase-1; D000252:Calcium-Transporting ATPases