Xeroderma pigmentosum group E binding factor recognizes a broad spectrum of DNA damage.
Xeroderma pigmentosum complementation group E binding factor (XPE-BF) is a damaged DNA binding protein that is deficient in a subset of patients from complementation group E of xeroderma pigmentosum. The protein recognizes various forms of DNA damage including some cyclobutane pyrimidine dimers, 6-4 photoproducts, cis-diamminedichloroplatinum(II) adducts, and single-stranded DNA. We now show that it also recognizes damage induced by nitrogen mustard; N-methyl-N'-nitro-N-nitrosoguanidine, and depurination, but has no detectable affinity for DNA adducts generated by trans-diamminedichloroplatinum(II), 4-nitroquinoline-N-oxide, 8-methoxypsoralen, or enzymatically methylated cytosine and adenine. The failure to recognize 4-nitroquinoline-N-oxide and 8-methoxypsoralen adducts is consistent with previous reports that XPE cells carry out wild-type levels of repair synthesis after DNA damage by those drugs. These results demonstrate that XPE-BF is a versatile damage recognition protein, but suggest that other proteins must contribute to the recognition of DNA lesions for the human excision repair pathway.
Payne
A
A
Department of Medicine, Stanford University School of Medicine, CA 94305.
Chu
G
G
D015112:4-Nitroquinoline-1-oxide / Q000378:metabolism / Q000633:toxicity; D002945:Cisplatin / Q000378:metabolism / Q000633:toxicity; D004247:DNA / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D008466:Mechlorethamine / Q000378:metabolism / Q000633:toxicity; D008730:Methoxsalen / Q000378:metabolism / Q000633:toxicity; D008769:Methylnitronitrosoguanidine / Q000378:metabolism / Q000633:toxicity; D009153:Mutagens / Q000378:metabolism / Q000633:toxicity; D014466:Ultraviolet Rays; D014983:Xeroderma Pigmentosum / Q000235:genetics / Q000378:metabolism*
C091435:DDB1 protein, human; D004268:DNA-Binding Proteins; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; C034697:transplatin; D008466:Mechlorethamine; D015112:4-Nitroquinoline-1-oxide; D004247:DNA; D002945:Cisplatin; D008730:Methoxsalen