Growth hormone promotes human T cell adhesion and migration to both human and murine matrix proteins in vitro and directly promotes xenogeneic engraftment.
Recombinant human growth hormone (rhGH) promotes human T cell engraftment in mice with severe combined immunodeficiency, suggesting that rhGH may have effects on T cell adhesion and migration in vivo. The ability of rhGH to directly affect the adhesion capacity of human T cells to a variety of human or murine adhesion molecules and extracellular matrix proteins was examined. rhGH induced significant human T cell adherence to both human and murine substrates via either beta 1 or beta 2 integrin molecules. rhGH was capable of inducing significant migration of resting and activated human T cells and their subsets. Most of the migratory response to rhGH was chemokinetic rather than chemotactic. In vivo engraftment studies in severe combined immunodeficiency mice receiving human T cells revealed that treatment with rhGH resulted in improved thymic engraftment, whereas treatment with non-human-reactive ovine GH demonstrated no significant effects. These data demonstrate that rhGH directly augments human T cell trafficking to peripheral murine lymphoid tissues. rhGH appears to be capable of directly altering the adhesive and migratory capacity of human T cells to molecules of either murine or human origin. Therefore, GH may, under either isogeneic or xenogeneic conditions, play a role in normal lymphocyte recirculation.
Taub
D D
DD
Clinical Services Program, Program Resources, Inc./DynCorp, Frederick, Maryland 21702-1201.
Tsarfaty
G
G
Lloyd
A R
AR
Durum
S K
SK
Longo
D L
DL
Murphy
W J
WJ
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D005353:Fibronectins / Q000502:physiology; D013006:Growth Hormone / Q000494:pharmacology*; D006801:Humans; D019012:Integrin beta1; D016023:Integrins / Q000502:physiology; D018799:Intercellular Adhesion Molecule-1; D051379:Mice; D016513:Mice, SCID; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000502:physiology / Q000637:transplantation; D014183:Transplantation, Heterologous; D019010:Vascular Cell Adhesion Molecule-1
D015815:Cell Adhesion Molecules; D005353:Fibronectins; D019012:Integrin beta1; D016023:Integrins; D011994:Recombinant Proteins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D013006:Growth Hormone