The structure of the O-specific polysaccharide of Hafnia alvei strain 1216.
The O-specific polysaccharide of Hafnia alvei strain 1216 is composed of D-galactose, D-glucuronic acid, 2-acetamido-2-deoxy-D-glucose, 3,6-dideoxy-3-[(R)-3-hydroxybutyramido]-D-glucose, and O-acetyl groups in the ratios 1:1:2:1:1. On the basis of sugar and methylation analyses of the intact and chemically degraded (O-deacetylated, carboxyl-reduced, Smith-degraded) polysaccharide and 1H and 13C NMR spectroscopy, including 2D shift-correlated (COSY, relayed COSY, 13C, 1H-COSY) and 1D NOE spectroscopy, it was concluded that the O-antigen is built up of linear pentasaccharide units having the following structure: [formula: see text]
Katzenellenbogen
E
E
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw.
Romanowska
E
E
Shashkov
A S
AS
Kocharova
N A
NA
Knirel
Y A
YA
Kochetkov
N K
NK
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D004755:Enterobacteriaceae / Q000737:chemistry* / Q000276:immunology; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000032:analysis; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D002247:Carbon Isotopes; D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D009005:Monosaccharides; D019081:O Antigens; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial