A simple and inexpensive chromatographic method for the purification of gamma-globulin from human serum.
Inexpensive cation- and anion-exchangers based on continuous polymer beds were prepared directly in a plastic syringe. These beds are unique in the sense that they are synthesized by a very simple and cost-effective procedure; for instance, no preparation of beads is required as in conventional methods. Highly purified gamma-globulin could be prepared easily by step-wise elution accomplished by forcing buffers of increasing salt concentration through the column with the aid of the plunger.
Mohammad
J
J
Department of Biochemistry, University of Uppsala, Sweden.
Hjertén
S
S
D002852:Chromatography, Ion Exchange / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D003362:Cost-Benefit Analysis; D006801:Humans; D005719:gamma-Globulins / Q000302:isolation & purification*
D005719:gamma-Globulins