title: Correlations between changes in membrane capacitance induced by changes in ionic environment and the conductance of channels incorporated into bilayer lipid membranes.
abstract: The action of metal polycations and pH on ionic channels produced in bilayer lipid membranes (BLM) by three different toxins was studied by measuring membrane capacitance and channel conductance. Here, we show that critical concentrations of Cd2+, La3+ or Tb3+ induce complex changes in membrane capacitance. The time course of capacitance changes is similar to the time course of channel blocking by these ions at low concentration. No changes in BLM capacitance or conductance were observed in the range of pH 5.8-9.0. A pH shift from 7.4 to 3-4 or 11-12 induced large changes in BLM capacitance and channel conductance. For all studied channel-forming proteins, the initial capacitance increase preceded the conductance decrease caused by addition of polycations or by a change in pH. A close relationship between membrane lipid packing and ion channel protein is suggested.
authors.affiliation: Department of Neurochemistry, A.V. Palladin Biochemistry Institute, Kiev, Ukraine.
authors.forename: A N
authors.initials: AN
authors.lastname: Chanturiya
authors.forename: H V
authors.initials: HV
authors.lastname: Nikoloshina
mesh_terms: D002104:Cadmium / Q000494:pharmacology; D003064:Cnidarian Venoms / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000737:chemistry* / Q000187:drug effects / Q000502:physiology*; D007811:Lanthanum / Q000494:pharmacology; D008051:Lipid Bilayers / Q000737:chemistry*; D008564:Membrane Potentials / Q000502:physiology*; D008567:Membranes, Artificial; D013111:Spider Venoms / Q000032:analysis*; D013725:Terbium / Q000494:pharmacology; D013997:Time Factors
chemical_list: D003064:Cnidarian Venoms; D007473:Ion Channels; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D013111:Spider Venoms; D002104:Cadmium; D013725:Terbium; C032104:toxin III (Anemonia sulcata); C020763:alpha-latrotoxin; D007811:Lanthanum