Dephosphorylation of tau protein from Alzheimer's disease patients.
Tau protein-prepared post-mortem from brains of Alzheimer's disease patients was treated with protein phosphatase 1 catalytic subunit, 2A catalytic subunit, and 2B (calcineurin). Dephosphorylation was monitored by immunoblotting with two monoclonal antibodies, TAU-1 and SMI31, which recognize in tau the dephospho- and phospho-states, respectively, of proline-directed protein kinase phosphorylation sites. Out of the three enzymes tested, protein phosphatase 2A was only effective in dephosphorylating tau at these Alzheimer-type epitopes.
Szücs
K
K
Department of Medical Chemistry, University School of Medicine, Debrecen, Hungary.
Ledesma
M D
MD
Dombrádi
V
V
Gergely
P
P
Avila
J
J
Friedrich
P
P
D000544:Alzheimer Disease / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D002540:Cerebral Cortex / Q000201:enzymology / Q000378:metabolism; D000939:Epitopes / Q000276:immunology / Q000378:metabolism; D006801:Humans; D015151:Immunoblotting; D010749:Phosphoprotein Phosphatases / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation; D038461:Proline-Directed Protein Kinases; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2; D017346:Protein Serine-Threonine Kinases / Q000276:immunology / Q000378:metabolism; D016875:tau Proteins / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D016875:tau Proteins; D038461:Proline-Directed Protein Kinases; D017346:Protein Serine-Threonine Kinases; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1; D054648:Protein Phosphatase 2