Role of CD40 antigen and interleukin-2 in T cell-dependent human B lymphocyte growth.
In the present study, we examined the participation of CD40 ligand (L)-CD40 interaction in T cell-dependent B cell responses. To this end, purified B lymphocytes were cultured over irradiated CD4+ cloned T cells activated with immobilized anti-CD3 antibody. The anti-CD40 mAb 89 strongly blocked, in a specific fashion, both proliferation and Ig secretion of tonsil B cells. Interestingly, proliferation of surface (s)IgD+ B cell was significantly less inhibited by anti-CD40 than that of sIgD- cells. Preactivated T cells induced B cells to grow and secrete immunoglobulins preferentially in response to IL-2. This contrasts with the CD40 system where B cells are essentially responsive to IL-4 and IL-10 but not to IL-2 alone. Collectively, these data indicate that CD40L-CD40 interaction plays an important role in IL-2 mediated T cell-dependent B cell responses. However, the activation of a subset of sIgD+ cells may be independent of this interaction.
Blanchard
D
D
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Gaillard
C
C
Hermann
P
P
Banchereau
J
J
D015703:Antigens, CD / Q000502:physiology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000502:physiology*; D016175:B-Lymphocyte Subsets / Q000166:cytology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D019013:CD40 Antigens; D023201:CD40 Ligand; D006801:Humans; D007072:Immunoglobulin D / Q000276:immunology; D007376:Interleukin-2 / Q000502:physiology*; D007378:Interleukins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008211:Lymphocyte Cooperation; D008562:Membrane Glycoproteins / Q000502:physiology
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D019013:CD40 Antigens; D007072:Immunoglobulin D; D007376:Interleukin-2; D007378:Interleukins; D008562:Membrane Glycoproteins; D023201:CD40 Ligand