[Protein determination by binding with the dye Coomassie brilliant blue G-250].
Possible applications of the Coomassie brilliant blue G-250 dye method for protein assay are reviewed. The physico-chemical and optical properties of the dye are considered in detail. The data on the protein-binding mechanism are generalized. Modifications of the Coomassie blue G-250 quantitative protein assays are critically considered. The applications of the method in medicine, biochemistry and biotechnology are discussed.
Gasparov
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Degtiar'
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D000818:Animals; D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011506:Proteins / Q000378:metabolism*; D012015:Reference Standards; D012394:Rosaniline Dyes / Q000737:chemistry / Q000378:metabolism*; D013057:Spectrum Analysis; D013194:Staining and Labeling
D011506:Proteins; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue