GDNF: existence of a second transcript in the brain.
The detection of the glial cell-line derived neurotrophic factor (GDNF) mRNA by RT-PCR in dissociated cell culture of rat embryonic or post-natal brain allowed the amplification of a doublet. The major band corresponded to the expected size and the minor one to a shorter product. We cloned and sequenced the latter product, and thus identified a mRNA potentially encoding for an isoform of the initially described precursor protein involved in GDNF synthesis.
Cristina
N
N
Laboratoire de Physiologie, Inserm U318, C.H.U. de Grenoble, France.
Chatellard-Causse
C
C
Manier
M
M
Feuerstein
C
C
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000502:physiology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D051100:Glial Cell Line-Derived Neurotrophic Factor; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D017421:Sequence Analysis; D014158:Transcription, Genetic / Q000235:genetics*
C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger