title: Pre-stimulation of the human bone marrow CD34+ cells before storage at 4 degrees C with the early acting cytokines enhance their survival and increase proliferative potential. Transplantological implications.
abstract: The aim of this study was to evaluate whether short prestimulation of the human bone marrow CD34+ cells before storage at 4 degrees C with early acting cytokines would improve their subsequent survival and clonogenecity. To address this question the cells were placed in iscove medium supplemented with 20% BCS and stimulated for 24 hours in 37 degrees C incubator with different combination of the early acting cytokines (KL, IL-1 beta, IL-3, IL-6). Subsequently the cells were shifted to 4 degrees C. On day 6 the cells were plated in methylcelulose cultures and stimulated to growth CFU-GM colonies. It had been found that 24 hours long pre-stimulation with early acting cytokines significantly enhances the survival of the CD34+ clonogeneic progenitors stored subsequently at 4 degrees C. The CD34+ cell prestimulated for 24 hours at 37 degrees C with KL + IL-3 and than shifted to 4 degrees C formed more than twice as much CFU-GM colonies in comparison to the cells stored whole time at 4 degrees C even in the presence of KL + IL-3. Therefore we suggest, that if there is a need to store bone marrow cells before transplantation for short time they should be prestimulated with early acting cytokines for 24 hours in liquid culture at 37 degrees C first and than placed at 4 degrees C. The data presented in this paper should be in the future confirmed in in vivo SCID animal model to evaluate the repopulation ability of marrow cells pre-stimulated before short term storage at 4 degrees C with early acting cytokines to enhance the proliferative potential of the transplant and to shorten the dangerous aplastic phase in the patients after transplantation.
authors.affiliation: Department of Immunology CSK WAM, Warsaw, Poland.
authors.forename: M Z
authors.initials: MZ
authors.lastname: Ratajczak
authors.forename: W I
authors.initials: WI
authors.lastname: Kuczyński
authors.forename: T
authors.initials: T
authors.lastname: Skórski
authors.forename: J
authors.initials: J
authors.lastname: Ratajczak
mesh_terms: D015703:Antigens, CD; D018952:Antigens, CD34; D001853:Bone Marrow / Q000187:drug effects / Q000276:immunology*; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D015925:Cryopreservation; D016207:Cytokines / Q000494:pharmacology*; D006801:Humans; D014181:Transplantation Immunology
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D016207:Cytokines