Matrix-assisted laser desorption mass spectrometric analysis of a pegylated recombinant protein.
Matrix-assisted laser desorption ionization mass spectrometry (MALDI) has been investigated as a technique for the characterization of recombinant stem cell factor that had been covalently modified with polyethylene glycol (PEG) chains. The attachment of PEG chains produces a heterogeneous mixture of protein species that differ by 6000 Da. These differences in molecular weight could be readily determined by MALDI. The potential of MALDI as a general strategy for characterizing PEG-modified proteins is discussed.
Watson
E
E
Amgen, Thousand Oaks, CA.
Shah
B
B
DePrince
R
R
Hendren
R W
RW
Nelson
R
R
D001709:Biotechnology; D016298:Hematopoietic Cell Growth Factors / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D019089:Stem Cell Factor
D016298:Hematopoietic Cell Growth Factors; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D011092:Polyethylene Glycols