A gene expression vector useful for protein purification and studies of protein-protein interaction.
This report describes the development of a gene expression vector that adds three features to the C-terminus of the putative synthesized protein: (1) a protein kinase A recognition domain allowing the protein to be radio-labeled in vitro, (2) an epitope marker for immunocharacterization of the protein with a commercial monoclonal antibody, and (3) a (His)6 block facilitating purification of the protein by metal chelating affinity chromatography. These features make it convenient to perform biochemical and functional analysis of the protein of interest.
Zhao
L J
LJ
Marion Merrell Dow Foundation, Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66160-7424.
Narayan
O
O
D000595:Amino Acid Sequence; D001483:Base Sequence; D002846:Chromatography, Affinity; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004274:DNA, Recombinant; D000939:Epitopes / Q000235:genetics; D015693:Gene Products, nef / Q000235:genetics* / Q000378:metabolism; D005822:Genetic Vectors; D015497:HIV-1; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011485:Protein Binding / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D054311:nef Gene Products, Human Immunodeficiency Virus
D004274:DNA, Recombinant; D000939:Epitopes; D015693:Gene Products, nef; D011994:Recombinant Proteins; D054311:nef Gene Products, Human Immunodeficiency Virus; D017868:Cyclic AMP-Dependent Protein Kinases