[A new liver support system composed of functional human cells and a radial-flow bioreactor].
An artificial liver will be useful for the treatment of acute hepatic failure and a bridge of liver transplantation. The current reports suggest that the hybrid type of artificial liver composed of functional human liver cells and a bioreactor is practical for clinical use. In the present study, we succeeded high density culture on a large-scale of human functional hepatoma (JHH-7) using a newly developed radial flow packed-bed bioreactor. Since the shear stress of this bioreactor is lower than the other type, high density culture without cell damage is possible. JHH-7 cells produced large amounts of human albumin and other liver specific proteins, and then have the function of ammonia metabolism in the system. This study suggests that a radial flow bioreactor will be developed as a new type of artificial liver.
Kawada
M
M
Department of Internal Medicine (I), Jikei University School of Medicine, Tokyo, Japan.
Nagamori
S
S
Aizaki
H
H
Fukaya
K
K
Niiya
M
M
Matsuura
T
T
Sujino
H
H
Hasumura
S
S
Shimizu
K
K
Yoshida
H
H
D000418:Albumins / Q000096:biosynthesis; D000641:Ammonia / Q000378:metabolism; D001187:Artificial Organs; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008113:Liver Neoplasms / Q000473:pathology; D010101:Oxygen Consumption; D014407:Tumor Cells, Cultured; D014508:Urea / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000096:biosynthesis
D000418:Albumins; D000509:alpha-Fetoproteins; D000641:Ammonia; D014508:Urea; D005947:Glucose