Effects of spermidine synthase inhibition on cytoskeletal organization in cultured chick embryo fibroblasts.
The administration of bis-cyclohexylammonium sulphate (BCHS), an inhibitor of spermidine synthase, to cultured chick embryo fibroblasts provoked alterations in cell morphology, a marked disorganization of microfilaments and changes in microtubule network structure. In addition, the rate of microtubule reappearance, after disrupting them with colchicine, was impaired by BCHS. These responses to BCHS were prevented by spermidine addition, which thus suggests an involvement of spermidine in microtubule and microfilament organization.
Caruso
A
A
Institute of Histology and General Embryology, University of Ferrara, Italy.
Pellati
A
A
Bosi
G
G
Arena
N
N
Stabellini
G
G
D008841:Actin Cytoskeleton / Q000648:ultrastructure; D000199:Actins / Q000648:ultrastructure; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology; D003514:Cyclohexylamines / Q000494:pharmacology*; D003599:Cytoskeleton / Q000648:ultrastructure*; D005347:Fibroblasts / Q000187:drug effects* / Q000648:ultrastructure*; D008870:Microtubules / Q000648:ultrastructure; D011436:Spermidine Synthase / Q000037:antagonists & inhibitors*; D014404:Tubulin / Q000648:ultrastructure
D000199:Actins; D003514:Cyclohexylamines; D014404:Tubulin; C076758:bis(cyclohexylammonium)sulfate; D011436:Spermidine Synthase; D003078:Colchicine