[The determination of optimal regimens for producing Klebsiella antigenic erythrocytic immunoreagents].
The optimum conditions for obtaining sensitive K- and D-serovar-specific Klebsiella antigenic erythrocyte diagnosticum have been established. The methods of choice are sensitization with lipopolysaccharide antigens at pH 5.0 and in a neutral medium at a temperature of 45 degrees C for an hour. The reagents thus obtained have proved to be specific when tested in the passive hemagglutination test with antisera to Shigella, Salmonella and Escherichia.
Denisov
G I
GI
Aleshin
A I
AI
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000276:immunology / Q000302:isolation & purification*; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology*; D006386:Hemagglutination Tests; D007114:Immunization / Q000379:methods; D007202:Indicators and Reagents; D007710:Klebsiella Infections / Q000175:diagnosis; D007711:Klebsiella pneumoniae / Q000276:immunology*; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D012680:Sensitivity and Specificity
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D000939:Epitopes; D007202:Indicators and Reagents; C030659:K antigens; D019081:O Antigens; D011135:Polysaccharides, Bacterial