Neuronal survival is associated with 72-kDa heat shock protein expression after transient middle cerebral artery occlusion in the rat.
Induction of the 72-kDa heat shock protein expression is thought to protect neurons against the subsequent effects of ischemia. However, it is not clear whether the induction of 72-kDa heat shock protein expression by an ischemic event improves neuronal survival. To address this question, we outlined the temporal profile of neuronal induction and expression of the 72-kDa heat shock protein in a model of transient focal ischemia in the rat. Fifty two adult Wistar rats were subjected to middle cerebral artery occlusion of 2 h duration. At 0.5, 3, 6, 9, 12, 24, 48, 96 and 168 h after reopening the artery, coronal brain sections were analyzed using both immunohistochemical methods and hematoxylin and eosin staining to determine the topographic and cellular distribution of the 72-kDa heat shock protein, as well as the extent of neuronal damage. Immunoreactivity to the 72-kDa heat shock protein was not detected in neurons that were destined to become necrotic, and were located in the ischemic core of the brain lesions. However, 72-kDa heat shock protein expression was evident in morphologically intact neurons located in the peripheral zone. The earliest neuronal expression of 72-kDa heat shock protein was detected in animals in which the 2 h occlusion of the middle cerebral artery was followed by 6 h recirculation; the intensity of the 72-kDa heat shock protein immunoreactivity peaked at 48 h, and progressively disappeared 7 days after the ischemic reperfusion event.(ABSTRACT TRUNCATED AT 250 WORDS)
Li
Y
Y
Department of Neurology, Henry Ford Health Science Center, Detroit, MI 48202.
Chopp
M
M
Zhang
Z G
ZG
Zhang
R L
RL
Garcia
J H
JH
D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D017536:Carotid Artery, Common / Q000502:physiology; D002342:Carotid Artery, External / Q000502:physiology; D002343:Carotid Artery, Internal / Q000502:physiology; D002470:Cell Survival; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D009336:Necrosis / Q000473:pathology; D009474:Neurons / Q000502:physiology*; D016612:Paraffin Embedding; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling
D006360:Heat-Shock Proteins