Enhanced MYCN expression and isochromosome 17q in pineoblastoma cell lines.
We have established two cell lines, PER-452 and PER-453, from an 8-month-old girl with an extensive pineoblastoma. Characterization of these lines revealed that the proto-oncogenes MYC and MYCN were not amplified, but both cell lines showed MYCN expression comparable to a cell line with 200-fold MYCN amplification. Both cell lines contained an i(17q). These results support the concept that pineoblastomas belong to a larger group of primitive neuroectodermal tumors of the central nervous system. These two cell lines provide a unique opportunity to investigate the molecular genetic mechanisms underlying these neoplasms further.
Kees
U R
UR
Division of Children's Leukemia and Cancer Research, Western Australian Research Institute for Child Health, Princess Margaret Hospital, Perth.
Biegel
J A
JA
Ford
J
J
Ranford
P R
PR
Peroni
S E
SE
Hallam
L A
LA
Parmiter
A H
AH
Willoughby
M L
ML
Spagnolo
D
D
D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002869:Chromosome Aberrations; D002886:Chromosomes, Human, Pair 17 / Q000648:ultrastructure*; D017809:Fatal Outcome; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D006801:Humans; D007223:Infant; D010870:Pineal Gland; D010871:Pinealoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured