Localization of four antigenic sites involved in Venezuelan equine encephalomyelitis virus protection.
Stable neutralization and protection escape variants of a virulent strain (Trinidad Donkey) of the VEE virus were selected by monoclonal antibodies (MAbs). Determination of nucleotide sequences of nine variants revealed a clustering of single mutations in four regions of the E1 and E2 glycoproteins. Involvement of amino acid residues 206 (site E1-1), 57 and 59 (site E2-2), 180, 182, 213, 214 and 216 (site E2-6) and 232 (site E2-3) in protective epitopes was demonstrated.
Agapov
E V
EV
Institute of Molecular Biology, State Research Center of Virology and Biotechnology Vector, Koltsovo, Russia.
Razumov
I A
IA
Frolov
I V
IV
Kolykhalov
A A
AA
Netesov
S V
SV
Loktev
V B
VB
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D002522:Chlorocebus aethiops; D003062:Codon / Q000235:genetics; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D000939:Epitopes / Q000032:analysis*; D014644:Genetic Variation; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D017354:Point Mutation; D014709:Vero Cells; D014774:Virulence
D000911:Antibodies, Monoclonal; D003062:Codon; D000939:Epitopes