Alkaline phosphatase staining of pig and sheep epiblast cells in culture.
To define better the characteristics of pig and sheep epiblast cells in culture, the cells were tested for the presence of alkaline phosphatase (AP), a biochemical marker characteristic of mouse embryonic stem cells. Pig and sheep epiblast cells were positive for AP staining both at isolation from the blastocyst and after primary in vitro culture. The innermost portion of the attendant endoderm surrounding the epiblast was also positive for AP staining during primary culture. AP staining was lost upon differentiation or senescence of the epiblast cells. Also, all differentiated epiblast-derived cell cultures were negative for AP staining, with the exception of neuron-like cultures. Epiblast-like cells were cultured from day 10 (pig) and day 13 (sheep) embryonic discs, and these cells were also AP positive until they differentiated. Trophectoderm-endoderm-like cells from embryonic discs were AP negative or weakly positive. AP is a convenient marker for undifferentiated pig and sheep epiblast cells in culture when used in conjunction with cell morphology analysis.
Talbot
N C
NC
U.S. Department of Agriculture, Beltsville Agricultural Research Center, MD 20705.
Rexroad
C E
CE
Pursel
V G
VG
Powell
A M
AM
D000469:Alkaline Phosphatase / Q000032:analysis*; D000818:Animals; D015415:Biomarkers; D001755:Blastocyst / Q000166:cytology* / Q000201:enzymology; D002454:Cell Differentiation; D002478:Cells, Cultured; D016922:Cellular Senescence; D003979:Diazonium Compounds; D004475:Ectoderm / Q000166:cytology / Q000201:enzymology; D004707:Endoderm / Q000166:cytology / Q000201:enzymology; D009284:Naphthols; D009928:Organ Specificity; D010755:Organophosphates; D012756:Sheep / Q000196:embryology*; D013045:Species Specificity; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology* / Q000201:enzymology; D013552:Swine / Q000196:embryology*; D014327:Trophoblasts / Q000166:cytology / Q000201:enzymology
D015415:Biomarkers; D003979:Diazonium Compounds; D009284:Naphthols; D010755:Organophosphates; C084845:naphthol AS-MX phosphate; C027766:Fast Red TA; D000469:Alkaline Phosphatase