The two types of mRNAs for neurofibromin isoforms produced by von Recklinghausen neurofibromatosis (NF1) gene: analysis in human astrocytic tumors.
Two types of mRNAs for neurofibromin isoforms, which are produced by Von Recklinghausen neurofibromatosis (NF1) gene, have so far been identified. In the present study, we analyzed the differential expression of the two NF1 mRNAs in 16 cases of human astrocytic tumors and in surrounding normal tissues by the RNA-polymerase chain reaction. Astrocytic tumors predominantly expressed type II NF1 mRNA, whereas it was type I isoform that was predominantly expressed in the normal tissues. These results suggested that the increased type II NF1 protein might play an important role in the growth of astrocytic tumors.
Tokuyama
T
T
Department of Biochemistry, Hamamatsu University School of Medicine, Japan.
Uemura
K
K
Fujita
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000235:genetics*; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics*; D002648:Child; D005260:Female; D016514:Genes, Neurofibromatosis 1; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D025542:Neurofibromin 1; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*
D025542:Neurofibromin 1; D011506:Proteins; D012333:RNA, Messenger