Detection of Bartonella (Rochalimaea) quintana by routine acridine orange staining of broth blood cultures.
Bartonella quintana was isolated from 34 BACTEC nonradiometric aerobic resin blood cultures for 10 adults. Nine patients were initially diagnosed by routine acridine orange staining of routine cultures that had been incubated for 8 days. All subcultures grew on chocolate agar within 3 to 12 days (median, 6 days). The PLUS 26 high-volume aerobic resin medium, combined with acridine orange stain and subculture, is an effective system for detection and isolation of B. quintana from blood.
Larson
A M
AM
Department of Laboratory Medicine, Harborview Medical Center, University of Washington, Seattle 98104.
Dougherty
M J
MJ
Nowowiejski
D J
DJ
Welch
D F
DF
Matar
G M
GM
Swaminathan
B
B
Coyle
M B
MB
D000165:Acridine Orange; D000328:Adult; D000332:Aerobiosis; D016470:Bacteremia / Q000382:microbiology*; D001426:Bacterial Proteins / Q000032:analysis; D001431:Bacteriological Techniques; D003470:Culture Media; D005227:Fatty Acids / Q000032:analysis; D006801:Humans; D012287:Rickettsiaceae / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D013045:Species Specificity; D013194:Staining and Labeling; D013997:Time Factors; D014205:Trench Fever / Q000382:microbiology*
D001426:Bacterial Proteins; D003470:Culture Media; D005227:Fatty Acids; D000165:Acridine Orange