[Role of neutrophils in warm renal ischemia and reperfusion injury in the rat].
This study attempts to determine whether the circulating neutrophils play a role in mediating injury resulting from renal ischemia, using rats treated with cyclophosphamide (CY) and/or granulocyte colony-stimulating factor (G-CSF). The neutrophil counts immediately before 60-minute ischemia decreased by 89% in the CY-treated rats in comparison with the untreated animals (p < 0.01). In the rats given G-CSF with CY, the neutrophil counts significantly increased over that of the CY-treated rats (p < 0.01). The serum creatinine level 24 hours after reperfusion amounted to 4.42 +/- 0.37 mg/dl in the control rats and 2.63 +/- 0.29 mg/dl in the CY-treated rats (p < 0.01). In 19 rats, 10 controls and the 9 CY-treated, the neutrophil counts immediately before ischemia correlated well with the serum creatinine levels 24 hours after reperfusion (r = 0.597; p < 0.01). Our results appear to indicate that neutrophils play an important role in warm ischemia and reperfusion injury of the kidney.
Tomikawa
S
S
Department of Surgery and Organ Transplantation, University of Tokyo, Japan.
Akiyama
N
N
Nagao
T
T
Uchida
H
H
D000818:Animals; D001831:Body Temperature; D003404:Creatinine / Q000097:blood; D003520:Cyclophosphamide / Q000494:pharmacology; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D007511:Ischemia / Q000097:blood*; D007668:Kidney / Q000098:blood supply*; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood*
D016179:Granulocyte Colony-Stimulating Factor; D003520:Cyclophosphamide; D003404:Creatinine