Detection of antigen-specific immune complexes in sera of gastric and esophageal cancer patients.
Using the murine monoclonal antibodies against human gastric cancer cell antigens designated MG7 and MGd1, we developed a sandwich ELISA to detect the levels of antigen-specific immune complexes (IC) in human sera. By this assay, only 6.7%(6/90) of healthy blood donors and 9.2%(6/65) of patients with chronic gastritis had antigen-specific IC in sera exceeding the cut-off value. Whereas 21.4%(6/28) of patients with colorectal cancer, 18.2%(4/22) of patients with hepatocellular cancer, 11.4%(4/35) of patients with lung cancer and 9.5%(2/21) of patients with breast cancer had elevated levels of the antigen-specific IC. In contrast, 58.7%(54/92) of patients with gastric cancer and 53.3%(24/45) of patients with esophageal cancer were positive for this antigen-specific IC. Determination of levels of antigen-specific IC in sera may be useful in serologic diagnosis of patients with gastric and esophageal cancer.
Li
G
G
Department of Digestive Disease, Xijing Hospital, Fourth Military Medical University, Xi'an.
Zhang
X Y
XY
Fan
D M
DM
Hu
J L
JL
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex / Q000097:blood*; D000951:Antigens, Neoplasm / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes; D004938:Esophageal Neoplasms / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm; D000939:Epitopes