Discrimination of Mycobacterium avium-Mycobacterium intracellulare strains by genomic DNA fingerprinting with a 16S rRNA gene probe.
Ribotyping was investigated as a means of distinguishing ten different serotyped reference strains and seven epidemiologically unrelated isolates of Mycobacterium avium-Mycobacterium intracellulare using a labelled 16S rDNA probe. Thirteen restriction enzymes were screened towards an accurate discrimination of strains. Two selected restriction enzymes (SacI and ClaI) enabled us to classify the 17 strains into ten ribotypes with an index of discrimination of 0.897. Typeability and reproducibility of the method reached 100%. The patterns obtained exhibited polymorphism of RE fragments within and outside the 16S rRNA gene and may be useful for epidemiological studies.
Peillon
R
R
Unité d'infectiologie, Institut Pasteur de Lyon, France.
Drouet
E B
EB
Bruneau
S
S
Panteix
G
G
Denoyel
G A
GA
De Montclos
H P
HP
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific; D008969:Molecular Sequence Data; D009162:Mycobacterium avium / Q000145:classification / Q000235:genetics*; D015269:Mycobacterium avium Complex / Q000145:classification / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015342:DNA Probes; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C024408:endodeoxyribonuclease SacI; C032824:ATCGAT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific