The chemokine, eotaxin, activates guinea-pig eosinophils in vitro and causes their accumulation into the lung in vivo.
Eotaxin is a novel C-C chemokine purified from the bronchoalveolar lavage (BAL) fluid of actively sensitised guinea-pigs after aerosol allergen challenge. In this study we show that eotaxin induced a dose-dependent increase in both intracellular-free calcium concentration ([Ca2+]i) and aggregation of guinea-pig eosinophils in vitro. Intradermally injected eotaxin induced the accumulation of [111In]eosinophils in naive guinea-pig skin in vivo, without oedema-inducing activity; the latter emerging in separate BAL fluid HPLC fractions. Aerosol exposure of naive guinea-pigs to eotaxin in vivo caused a selective increase in eosinophils in BAL fluid. Thus, eotaxin activates guinea-pig eosinophils in vitro and causes a selective eosinophil accumulation in the lung in vivo. Eotaxin and related molecules are potentially important endogenous signalling substances in allergic reactions in vivo.
Griffiths-Johnson
D A
DA
Department of Applied Pharmacology, National Heart and Lung Institute, London, UK.
Collins
P D
PD
Rossi
A G
AG
Jose
P J
PJ
Williams
T J
TJ
D000485:Allergens; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology*; D002118:Calcium / Q000378:metabolism; D002449:Cell Aggregation / Q000187:drug effects; D018946:Chemokine CCL5; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004804:Eosinophils / Q000187:drug effects / Q000502:physiology*; D006168:Guinea Pigs; D006801:Humans; D008168:Lung / Q000187:drug effects / Q000502:physiology*; D008222:Lymphokines / Q000737:chemistry; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D017386:Sequence Homology, Amino Acid
D000485:Allergens; D018946:Chemokine CCL5; D008222:Lymphokines; D002118:Calcium