title: Isolation of human CD34+ peripheral blood stem cells using an immunomagnetic positive selection system: prior monocyte depletion using a nylon-wool column.
abstract: An immunomagnetic separation system has been used to collect CD34+ cells in mobilized blood after treatment with a nylon-wool column. Cell purities were increased from 2.6% preseparation to 94.6% postseparation, with a mean yield 45.2% (n = 4). Forty percent of CD34+ cells separated by the immunomagnetic procedure formed colonies in the presence of hematopoietic growth factors in a limiting dilution assay. Eleven percent of these clones proliferated to over 10(5) cells and contained megakaryocytes.
authors.affiliation: Blood Transfusion Center, Tokai University Hospital, Kanagawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: M
authors.initials: M
authors.lastname: Noma
authors.forename: M
authors.initials: M
authors.lastname: Kidokoro
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Takei
authors.forename: S
authors.initials: S
authors.lastname: Kurashima
authors.forename: T
authors.initials: T
authors.lastname: Mukaiyama
authors.forename: S
authors.initials: S
authors.lastname: Kato
mesh_terms: D015703:Antigens, CD / Q000097:blood*; D018952:Antigens, CD34; D002454:Cell Differentiation / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D002999:Clone Cells; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation*; D007201:Indicator Dilution Techniques; D007958:Leukocyte Count; D009000:Monocytes / Q000166:cytology; D009757:Nylons
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D009757:Nylons