title: Identification of hair cell progenitors and intermitotic migration of their nuclei in the normal and regenerating avian inner ear.
abstract: Postembryonic production of sensory hair cells occurs in both normal and aminoglycoside-damaged avian inner ears. The cellular source and mechanism that results in new differentiated hair cells were investigated in the avian vestibular epithelia using three distinct cell-cycle-specific labeling methods to identify proliferating sensory epithelial cells. First, immunocytochemical detection of the proliferating cell nuclear antigen, an auxiliary protein of DNA polymerase, allowed labeling of cells in late G1, S, and early G2 phases of the cell cycle. Second, a pulse-fix tritiated thymidine autoradiographic protocol was used to identify cells in S phase of the cell cycle. Finally, Hoechst 33342, a fluorescent DNA stain, was used to identify epithelial cells in mitosis. The distribution of cells active in the cell cycle within the normal and ototoxin-damaged vestibular epithelium suggests that supporting cells within the sensory epithelia are the cellular precursors to the regenerated hair cells. Differences between the proliferation marker densities in control and damaged end organs indicate that the upregulation of mitotic activity observed after streptomycin treatment is due primarily to an increase in the number of dividing progenitor cells. The differences between the extent of ototoxic damage and the level of reparative proliferative response suggest a generalized stimulus, such as a soluble chemical factor, plays a role in initiating regeneration. Finally, after DNA replication is initiated, progenitor cell nuclei migrate from their original location close to the basement membrane to the lumenal surface, where cell division occurs. This pattern of intermitotic nuclear migration is analogous to that observed in the developing inner ear and neural epithelium.
authors.affiliation: Virginia Merrill Bloedel Hearing Research Center, University of Washington, Seattle 98195.
authors.forename: T T
authors.initials: TT
authors.lastname: Tsue
authors.forename: D L
authors.initials: DL
authors.lastname: Watling
authors.forename: P
authors.initials: P
authors.lastname: Weisleder
authors.forename: M D
authors.initials: MD
authors.lastname: Coltrera
authors.forename: E W
authors.initials: EW
authors.lastname: Rubel
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D006690:Bisbenzimidazole; D002467:Cell Nucleus / Q000502:physiology*; D002645:Chickens / Q000502:physiology*; D007758:Ear, Inner / Q000166:cytology / Q000502:physiology*; D006198:Hair Cells, Auditory / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D008938:Mitosis / Q000502:physiology*; D009687:Nuclear Proteins / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen; D012016:Reference Values; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D013936:Thymidine / Q000378:metabolism; D014722:Vestibule, Labyrinth / Q000187:drug effects / Q000473:pathology
chemical_list: D000617:Aminoglycosides; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D006690:Bisbenzimidazole; D013936:Thymidine