Analysis of streptomycin and dihydrostreptomycin in milk by liquid chromatography.
A method developed for the determination of the aminoglycoside antibiotics streptomycin and dihydrostreptomycin in tissues was applied to the analysis of fluid milk. Samples are extracted with 3.6% perchloric acid, and then injected onto a trace enrichment column, from which they are eluted onto a reversed-phase analytical column. The analytes are detected by fluorescence following postcolumn derivatization with 1,2-naphthoquinone-4-sulfonic acid. Recovery of analytes was in the range of 50-65% for skim or partially defatted fluid milk, while recoveries for homogenized whole milk were lower. Limits of quantitation were 10 ppb for streptomycin and 20 ppb for dihydrostreptomycin.
Gerhardt
G C
GC
Agriculture Canada, Health of Animals Laboratory, Saskatoon, SK.
Salisbury
C D
CD
MacNeil
J D
JD
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods* / Q000706:statistics & numerical data; D004096:Dihydrostreptomycin Sulfate / Q000032:analysis*; D008055:Lipids / Q000032:analysis; D008892:Milk / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013307:Streptomycin / Q000032:analysis*
D008055:Lipids; D004096:Dihydrostreptomycin Sulfate; D013307:Streptomycin