title: Ionic channel activity induced by fusion of Rhodospirillum rubrum chromatophores with a planar bilayer lipid membrane.
abstract: The present work concerns mechanisms of ionic conductivity of photosynthetic membranes. It is shown that reconstitution of vesicles of photosynthetic membranes (chromatophores) of purple bacteria Rhodospirillum rubrum into a planar bilayer lipid membrane leads to fluctuations of current showing the existence of a channel with a predominant conductance of approximately 230 pS in the presence of 100 mM KCl. Measurements under the conditions of KCl gradient prove that this channel is cation selective (PK/PCl = 7.2). Voltage inactivation of the channel is demonstrated which is prevented by treatment with trypsin.
authors.affiliation: A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russian Federation.
authors.forename: Y N
authors.initials: YN
authors.lastname: Antonenko
authors.forename: T I
authors.initials: TI
authors.lastname: Rokitskaya
authors.forename: E A
authors.initials: EA
authors.lastname: Kotova
authors.forename: A S
authors.initials: AS
authors.lastname: Taisova
mesh_terms: D020130:Bacterial Chromatophores / Q000648:ultrastructure*; D002462:Cell Membrane / Q000502:physiology*; D004553:Electric Conductivity; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000502:physiology*; D008051:Lipid Bilayers; D008561:Membrane Fusion; D010788:Photosynthesis; D011189:Potassium Chloride / Q000494:pharmacology; D012247:Rhodospirillum rubrum / Q000648:ultrastructure*; D014357:Trypsin / Q000494:pharmacology
chemical_list: D007473:Ion Channels; D008051:Lipid Bilayers; D011189:Potassium Chloride; D014357:Trypsin