title: Numerical chromosomal aberrations in thyroid tumors detected by double fluorescence in situ hybridization.
abstract: Double fluorescence in situ hybridization with DNA probes specific for the (peri)centromeric regions of chromosomes 3, 7, 9, 11, 12, 18, and X was performed on fresh isolated nuclei and frozen tissue sections prepared from 2 nodular hyperplasias, 2 adenomas, and 7 papillary carcinomas of the thyroid in order to detect numerical chromosomal changes. Numerical chromosomal aberrations were found in all malignant specimens examined. A consistent presence of at least two trisomies was detected in most cases, especially in the follicular variant specimens; the highest degree of trisomy was observed for chromosome 12. Isolated monosomies of moderate degree for different chromosomes were found in 1 adenoma and 2 papillary carcinomas. Severe monosomy of chromosome 9 was the only significant feature observed in the single metastatic papillary carcinoma.
authors.affiliation: Department of Genetics, Yale University Medical School, New Haven, Connecticut.
authors.forename: D
authors.initials: D
authors.lastname: Taruscio
authors.forename: M L
authors.initials: ML
authors.lastname: Carcangiu
authors.forename: T
authors.initials: T
authors.lastname: Ried
authors.forename: D C
authors.initials: DC
authors.lastname: Ward
mesh_terms: D000328:Adult; D000782:Aneuploidy; D002291:Carcinoma, Papillary / Q000235:genetics; D018265:Carcinoma, Papillary, Follicular / Q000235:genetics; D002467:Cell Nucleus / Q000648:ultrastructure; D002648:Child; D002877:Chromosomes, Human; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D008875:Middle Aged; D013964:Thyroid Neoplasms / Q000235:genetics*; D016606:Thyroid Nodule / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm