Gold sodium thiomalate down-regulates intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression on vascular endothelial cells.
We examined whether antirheumatic drugs alter cytokine- or lipopolysaccharide-induced expression of adhesion molecules on vascular endothelial cells. Human umbilical cord vein endothelial cells were co-cultured with various antirheumatic drugs in the presence of inflammatory cytokines, and adhesion molecule expression was measured by cell enzyme-linked immunosorbent assay and Northern blot analysis. Among these antirheumatic drugs, gold sodium thiomalate significantly inhibited intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression on vascular endothelial cells and suppressed cellular binding between human monocytic cell lines, including U937 and HL-60 cells, and interleukin-1 beta-stimulated vascular endothelial cells. It is speculated that down-regulation of adhesion molecules might be one of the novel mechanisms of action of gold sodium thiomalate.
Koike
R
R
First Department of Medicine, School of Medicine, Tokyo Medical and Dental Univeristy, Japan.
Miki
I
I
Otoshi
M
M
Totsuka
T
T
Inoue
H
H
Kase
H
H
Saito
I
I
Miyasaka
N
N
D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002460:Cell Line; D015536:Down-Regulation / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005982:Glutathione Transferase / Q000494:pharmacology; D006052:Gold Sodium Thiomalate / Q000494:pharmacology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007375:Interleukin-1 / Q000494:pharmacology; D019010:Vascular Cell Adhesion Molecule-1
D000305:Adrenal Cortex Hormones; D015815:Cell Adhesion Molecules; D007166:Immunosuppressive Agents; D007375:Interleukin-1; D019010:Vascular Cell Adhesion Molecule-1; D006052:Gold Sodium Thiomalate; D018799:Intercellular Adhesion Molecule-1; D005982:Glutathione Transferase