Enhancing effect of the 3'-untranslated region of tobacco mosaic virus RNA on protein synthesis in vitro.
In order to test the enhancing effect of the 3'-terminal untranslated region (3'-UTR) of tobacco mosaic virus (TMV) RNA on protein synthesis in vitro we used a chimeric mRNA construct containing TMV 5'-UTR (omega) and firefly luciferase mRNA. The addition of the TMV 3'-UTR to the chimeric mRNA construct results in a more than 3-fold stimulation of the synthesis of the functionally active protein in the wheat germ cell-free translation system. We have demonstrated that the proper length of the TMV 3'-terminal part is important for efficient translation; elongation of the TMV tail by 160 vector-derived nucleotides fully abolishes the stimulation effect of the TMV 3'-UTR in vitro.
Zeyenko
V V
VV
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow region.
Ryabova
L A
LA
Gallie
D R
DR
Spirin
A S
AS
D001483:Base Sequence; D002474:Cell-Free System; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004355:Drug Stability; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012313:RNA / Q000494:pharmacology; D012333:RNA, Messenger / Q000494:pharmacology*; D012367:RNA, Viral / Q000494:pharmacology*; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014764:Viral Proteins
D012333:RNA, Messenger; D012367:RNA, Viral; C039919:RNA, recombinant; D014764:Viral Proteins; D012313:RNA; D008156:Luciferases; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases