title: Structure of the murine CD40 ligand gene.
abstract: The mouse CD40 ligand (CD40L) gene was cloned, sequenced and characterized. DNA sequence analysis showed that the CD40L gene comprises five exons and four intervening introns, spread over 13-14 kb of genomic DNA. The putative site for initiation of mRNA transcription was identified at 67 bp upstream of the translation initiation (ATG) codon. The nucleotide sequence of the 5'-flanking region of this gene revealed the presence of several regulatory regions including a TATA-like box, an Sp1-like box and six potential NF-AT-like motifs. The 3'-untranslated region of the murine CD40L gene contained two ATTTA-elements which are thought to confer instability to the mRNA of many cytokines and two adjacent dinucleotide repeates, (CT)25 and (CA)45. These elements may play a role in the post-transcriptional regulation of CD40L gene expression.
authors.affiliation: Division of Immunology, Children's Hospital, Boston, MA 02115.
authors.forename: E N
authors.initials: EN
authors.lastname: Tsitsikov
authors.forename: N
authors.initials: N
authors.lastname: Ramesh
authors.forename: R S
authors.initials: RS
authors.lastname: Geha
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D001483:Base Sequence; D019013:CD40 Antigens; D023201:CD40 Ligand; D003001:Cloning, Molecular; D005091:Exons; D005786:Gene Expression Regulation; D007438:Introns; D008024:Ligands; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012045:Regulatory Sequences, Nucleic Acid
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D019013:CD40 Antigens; D008024:Ligands; D008562:Membrane Glycoproteins; D023201:CD40 Ligand