Importance of loops of mitochondrial ADP/ATP carrier for its transport activity deduced from reactivities of its cysteine residues with the sulfhydryl reagent eosin-5-maleimide.
The effects of various compounds such as the transport substrate ADP and the transport inhibitors carboxyatractyloside (CATR) and bongkrekic acid (BKA) on the labeling of cysteine residues in the ADP/ATP carrier of bovine heart submitochondrial particles by the SH reagent eosin-5-maleimide (EMA) were studied. Of the four cysteine residues in the carrier, the labeling of Cys159 by EMA progressed predominantly and rapidly, and those of Cys56 and Cys256 moderately, but Cys128 was not labeled, as we reported previously [Majima, E., et al. (1993) J. Biol. Chem. 268, 22181-22187]. ADP inhibited the labelings of Cys56, Cys159, and Cys256 by EMA. BKA markedly inhibited the labeling of Cys159 by EMA, and also the labeling of Cys256, but did not affect the labeling of Cys56, suggesting that it binds from the matrix side to a region close to Cys159 in the second loop facing the matrix space. CATR completely inhibited the labeling by EMA when added on the cytosolic side, but had no effect when added on the matrix side. From these results, the conformational changes of the carrier induced by CATR, BKA, and ADP are discussed. Furthermore, a mechanism of adenine nucleotide transport through the ADP/ATP carrier in association with change in its conformation is proposed.
Majima
E
E
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Shinohara
Y
Y
Yamaguchi
N
N
Hong
Y M
YM
Terada
H
H
D000227:Adenine Nucleotides / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D001865:Bongkrekic Acid / Q000494:pharmacology; D002417:Cattle; D003545:Cysteine / Q000378:metabolism; D004801:Eosine Yellowish-(YS) / Q000031:analogs & derivatives / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000201:enzymology / Q000378:metabolism; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism*; D008954:Models, Biological; D011487:Protein Conformation; D013367:Submitochondrial Particles / Q000201:enzymology / Q000378:metabolism
D000227:Adenine Nucleotides; D001865:Bongkrekic Acid; D001278:Atractyloside; D000244:Adenosine Diphosphate; C033531:eosin maleimide; D000255:Adenosine Triphosphate; D000226:Mitochondrial ADP, ATP Translocases; D003545:Cysteine; C003853:carboxyatractyloside; D004801:Eosine Yellowish-(YS)