title: Purification and structural characterization of parathyroid hypertensive factor.
abstract: Parathyroid hypertensive factor (PHF) has been purified from two sources of material: plasma of spontaneously hypertensive rats (SHRs) and culture medium from organ culture of SHR parathyroid glands. Chromatographic characteristics of PHF from these two sources are identical. Biological activity of PHF (assayed as the characteristic delayed hypertensive response in normotensive rats) is sensitive to degradation by treatment in base, and the enzymes trypsin, chymotrypsin, phospholipase C, and phospholipase D. PHF activity may also be extracted from source material with chloroform: methanol (4:1). A hypothetical structure for the active component of PHF is suggested. This is comprised of a peptide liked to a lysophospholipid.
authors.affiliation: Department of Physiology, University of Alberta, Edmonton, Canada.
authors.forename: C G
authors.initials: CG
authors.lastname: Benishin
authors.forename: R Z
authors.initials: RZ
authors.lastname: Lewanczuk
authors.forename: J
authors.initials: J
authors.lastname: Shan
authors.forename: P K
authors.initials: PK
authors.lastname: Pang
mesh_terms: D000818:Animals; D001681:Biological Assay; D001685:Biological Factors / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D002918:Chymotrypsin; D006868:Hydrolysis; D008297:Male; D008745:Methylation; D008970:Molecular Weight; D009924:Organ Culture Techniques; D010084:Oxidation-Reduction; D010280:Parathyroid Glands / Q000737:chemistry / Q000378:metabolism; D010739:Phospholipase D; D010741:Phospholipases A; D051381:Rats; D011918:Rats, Inbred SHR; D017207:Rats, Sprague-Dawley; D014357:Trypsin; D010738:Type C Phospholipases
chemical_list: D001685:Biological Factors; C064505:parathyroid hypertensive factor; D010741:Phospholipases A; D010738:Type C Phospholipases; D010739:Phospholipase D; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; D002918:Chymotrypsin; D014357:Trypsin