Micro-encapsulation of MDCK-ras-e cells prevents loss of E-cadherin invasion-suppressor function in vivo.
The invasion-suppressor molecule E-cadherin mediates Ca(2+)-dependent cell aggregation and prevents invasion. E-cadherin-positive Madin-Darby canine kidney (MDCK) cells that were non-invasive in vitro formed, upon i.p. injection, tumors that were invasive. Differentiated tubular tumor areas showed an intense immuno-signal for E-cadherin at intercellular contacts, whereas undifferentiated structures did not. Cell lines derived from such tumors turned out to be invasive in vitro and showed decreased Ca(2+)-dependent cell aggregation but no change in E-cadherin immunopositivity. This combination of phenotypes indicated a loss of the E-cadherin invasion-suppressor function. Micro-encapsulation of i.p.-injected cells prevented the loss of the E-cadherin invasion-suppressor function. We concluded that this loss in vivo was dependent upon immediate contacts between tumor cells and host cells or upon host factors that could not cross the capsule membrane.
Vandenbossche
G M
GM
Laboratory of Pharmaceutical Technology, University of Ghent, Belgium.
De Bruyne
G K
GK
Bruyneel
E A
EA
Clemminck
G
G
Vleminckx
K
K
Van Roy
F M
FM
Mareel
M M
MM
D000818:Animals; D015820:Cadherins / Q000032:analysis / Q000502:physiology*; D002449:Cell Aggregation / Q000502:physiology; D002450:Cell Communication / Q000502:physiology*; D002460:Cell Line; D002461:Cell Line, Transformed; D002463:Cell Membrane Permeability; D004285:Dogs; D004339:Drug Compounding; D007686:Kidney Tubules, Distal / Q000166:cytology* / Q000502:physiology*; D008175:Lung Neoplasms / Q000473:pathology / Q000556:secondary; D051379:Mice; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009374:Neoplasms, Experimental / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D013194:Staining and Labeling / Q000379:methods
D015820:Cadherins