Biological implications of estrogen and androgen effects on androgen receptor and its mRNA levels in human uterine endometrium.
It has been shown that some effects of testosterone are different from those of its 5 alpha-reduced metabolite, dihydrotestosterone. Briefly, activities of testosterone might be related to cellular differentiation, whereas dihydrotestosterone acts on cellular proliferation. The number of testosterone binding sites in the uterine endometrium was increased by estradiol dipropionate, and this increase was down-regulated by testosterone cypionate. Dihydrotestosterone-specific binding sites in the endometrium were not modulated by estradiol dipropionate and testosterone cypionate. The dissociation constants of the binding sites for testosterone and dihydrotestosterone were not altered by these steroids. Estradiol dipropionate with or without testosterone cypionate induced androgen receptor mRNA expression in the endometrium. In conclusion, testosterone might predominantly affect cellular differentiation in the endometrium.
Fujimoto
J
J
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
Nishigaki
M
M
Hori
M
M
Ichigo
S
S
Itoh
T
T
Tamaya
T
T
D000328:Adult; D000728:Androgens / Q000494:pharmacology*; D001483:Base Sequence; D015139:Blotting, Southern; D002454:Cell Differentiation / Q000187:drug effects; D017931:DNA Primers / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D013196:Dihydrotestosterone / Q000378:metabolism; D004717:Endometrium / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D011944:Receptors, Androgen / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D013739:Testosterone / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology
D000728:Androgens; D017931:DNA Primers; D004967:Estrogens; D012333:RNA, Messenger; D011944:Receptors, Androgen; D013196:Dihydrotestosterone; D013739:Testosterone; D004958:Estradiol; C016131:testosterone 17 beta-cypionate; C023482:estradiol dipropionate