An in vitro system for the study of slow axonal transport.
Here we present a model system which for the first time permits studies of slow axonal transport in vitro. Axonally transported proteins of rat vagus nerves were radiolabelled with [35S]methionine in the nodose ganglion in vitro and were incubated for up to 3 days in culture medium. Slowly transported proteins were analyzed by one- and two-dimensional polyacrylamide gel electrophoresis and identified on Western blots of two-dimensional gels with antibodies to actin and alpha-tubulin. The system will be valuable for pharmacological analysis of the mechanisms of slow transport.
McLean
W G
WG
Department of Pharmacology and Therapeutics, University of Liverpool, UK.
Kanje
M
M
Remgård
P
P
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001370:Axonal Transport; D001369:Axons / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D066298:In Vitro Techniques; D008715:Methionine / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013462:Sulfur Radioisotopes; D014630:Vagus Nerve / Q000502:physiology*
D009419:Nerve Tissue Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine