title: Transabdominal chorionic villus sampling in the second and third trimesters of pregnancy: chromosome quality, reporting time, and feto-maternal bleeding.
abstract: Transabdominal chorionic villus sampling (TA-CVS) was performed in 210 pregnancies from 13 to 38 weeks using a double-needle technique. The sampling success was comparable to first-trimester TA-CVS and the diagnostic success rate was 98.2 per cent for the short-term technique and 99.3 per cent for cultured villi. Two fetuses could not be karyotyped. We found the chromosome quality to be similar to that in the first trimester, comparing the number of G-bands and other chromosome attributes. There were no unintended losses in a group (n = 142) with no sonographic abnormality, except for one death in utero at 38 weeks, 20 weeks after sampling. Chromosomal aberrations were seen in 19 per cent of cases with abnormal sonograms (n = 58). One cases of a discordant karyotype was found (false-negative prediction of Down's syndrome by the short-term preparation). There were no cases of fetal demise due to feto-maternal bleeding. It is suggested that double-needle TA-CVS in advanced pregnancies combines the advantages of rapid karyotyping of chromosomes of good quality and low risk for the fetus, and seems to be easier to practise and is probably safer than cordocentesis.
authors.affiliation: Department of Obstetrics and Gynecology, Rigshospitalet, University of Copenhagen, Denmark.
authors.forename: S
authors.initials: S
authors.lastname: Smidt-Jensen
authors.forename: C
authors.initials: C
authors.lastname: Lundsteen
authors.forename: A M
authors.initials: AM
authors.lastname: Lind
authors.forename: K
authors.initials: K
authors.lastname: Dinesen
authors.forename: J
authors.initials: J
authors.lastname: Philip
mesh_terms: D015193:Chorionic Villi Sampling; D002869:Chromosome Aberrations / Q000175:diagnosis*; D002871:Chromosome Banding; D025063:Chromosome Disorders; D002875:Chromosomes / Q000648:ultrastructure*; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis*; D005331:Fetomaternal Transfusion / Q000209:etiology*; D006801:Humans; D007621:Karyotyping / Q000379:methods; D011247:Pregnancy / Q000097:blood; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D000509:alpha-Fetoproteins