[Dermal reactions in children with atopic dermatitis--changes in histamine/tryptase levels in skin chambers. Report 1].
In the present study, we used the skin chamber method to determine histamine and tryptase released by continuous loading of a single antigen on the skin in order to evaluate dermal reactions produced and thereby obtain greater insight into the role of type I allergic reactions in children with atopic dermatitis. The subjects were 46 children, 23 males and 23 females aged 10.6 +/- 4.3 years on the average, who were being treated at the National Children's Hospital for moderate or severer atopic dermatitis. Mite antigen stimulation was carried out by the skin chamber method upon admission and histamine and tryptase levels in collected antigen solution were determined. Histamine levels in the chamber were increased significantly at 6, 12 and 24 hrs after stimulation compared to the control levels (p < 0.01). Tryptase levels were increased 2 hrs after stimulation but decreased with time thereafter. Histamine and tryptase levels were significantly correlated 2 hrs after stimulation, with a correlation coefficient of 0.954 (p < 0.01). No significant correlation was observed 24 hrs after stimulation. These findings indicate that children with severe atopic dermatitis have great skin reactivity and very sensitive to stimulation by antigens. Mast cells and basophils are thought to be involved in immediate and delayed type reactions, respectively.
Tsubaki
T
T
Department of Pediatrics, Jikei University School of Medicine.
Saito
H
H
Iikura
Y
Y
D000293:Adolescent; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001491:Basophils / Q000276:immunology; D002648:Child; D053818:Chymases; D003876:Dermatitis, Atopic / Q000276:immunology*; D005260:Female; D006636:Histamine Release; D006801:Humans; D006968:Hypersensitivity, Delayed; D006969:Hypersensitivity, Immediate; D008297:Male; D008407:Mast Cells / Q000276:immunology; D008925:Mites / Q000276:immunology*; D012697:Serine Endopeptidases / Q000378:metabolism*; D012867:Skin / Q000276:immunology*; D012882:Skin Tests / Q000379:methods*; D053802:Tryptases
D000941:Antigens; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases