Involvement of antipain-sensitive protease activity in suppression of UV-mutagenicity by human interferon-alpha.
To study the relationship between the transient elevation of protease activity and hypomutability observed in hypermutable human RSa cells pretreated with human interferon (HuIFN)-alpha and then irradiated with far-ultraviolet light (UV), protease inhibitors capable of specifically inhibiting the activity were investigated. Of ten inhibitors tested, antipain showed the greatest inhibitory effect. Antipain also prevented the suppression of UV-mutagenicity by HuIFN-alpha in RSa and xeroderma pigmentosum-derived fibroblast cells, as shown by culturing cells in medium containing antipain immediately after UV exposure and evaluating the generation of clones resistant to ouabain- or 6-thioguanine-mediated cytotoxicity. Thus, an antipain-sensitive protease may be involved in the hypomutability induced by HuIFN-alpha.
Isogai
E
E
Department of Biochemistry, School of Medicine, Chiba University, Japan.
Suzuki
N
N
D000976:Antipain / Q000494:pharmacology; D002460:Cell Line; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D009154:Mutation / Q000187:drug effects; D010447:Peptide Hydrolases / Q000378:metabolism*; D011480:Protease Inhibitors / Q000494:pharmacology; D014466:Ultraviolet Rays
D016898:Interferon-alpha; D011480:Protease Inhibitors; D000976:Antipain; D010447:Peptide Hydrolases