title: Isolation and culture of brain endothelial cells and establishment of in vitro blood-brain barrier model.
authors.affiliation: Division of Neurosurgery, Tohoku University School of Medicine, Sendai, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kondo
authors.forename: S
authors.initials: S
authors.lastname: Imaizumi
authors.forename: I
authors.initials: I
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Yoshimoto
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D002463:Cell Membrane Permeability; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D046508:Culture Techniques / Q000379:methods; D003911:Dextrans; D004553:Electric Conductivity; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D008954:Models, Biological; D011205:Povidone; D016548:Prosencephalon / Q000098:blood supply*; D051381:Rats; D012822:Silicon Dioxide; D013997:Time Factors
chemical_list: D003911:Dextrans; C015219:fluorescein isothiocyanate dextran; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone; D016650:Fluorescein-5-isothiocyanate