Expression of adhesion molecules in endothelial cells during allogeneic bone marrow transplantation.
Endothelial cell activation during allogeneic bone marrow transplantation, mainly in acute graft-versus-host disease (aGvHD) was studied in 23 recipients and 5 controls using anti-von Willebrand factor (vWF) antibody, antibodies to endothelial leukocyte adhesion molecule-1 (ELAM-1), vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1), and anti-HLA-DQ antibody, by immunohistological staining of skin. vWF extravasation, ELAM-1 and VCAM-1 expression were present in most recipients with a cutaneous rash which was confirmed as an aGvHD by histological examination (documented aGvHD) (p = 0.005 for vWF extravasation and ELAM-1 expression and p = 0.03 for VCAM-1 expression in comparison with the controls). In recipients with a rash, the cases displaying vWF extravasation and ELAM-1 expression were significantly more numerous in those with a documented aGvHD than in those without histological features of aGvHD (p = 0.01). vWF extravasation and ELAM-1 occurred concomitantly (p < 0.01). This study demonstrates that, during the course of skin aGvHD following bone marrow transplantation, there is transient expression of ELAM-1 and VCAM-1 by endothelial cells and simultaneous vWF extravasation, indicating an intense inflammation with endothelial cell participation.
Shen
N
N
Haemostasis Laboratory, Pasteur Institute of Lyon, France.
Ffrench
P
P
Guyotat
D
D
Ffrench
M
M
Fiere
D
D
Bryon
P A
PA
Dechavanne
M
M
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D019040:E-Selectin; D004727:Endothelium / Q000166:cytology / Q000276:immunology; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology*; D006683:HLA-DQ Antigens / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D008297:Male; D008875:Middle Aged; D012871:Skin Diseases / Q000276:immunology*; D019010:Vascular Cell Adhesion Molecule-1; D014841:von Willebrand Factor / Q000378:metabolism
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D006683:HLA-DQ Antigens; D019010:Vascular Cell Adhesion Molecule-1; D014841:von Willebrand Factor; D018799:Intercellular Adhesion Molecule-1