Characterization of vasocortin-like proteins induced by dexamethasone in endothelial cells.
In this study we have shown that dexamethasone induces vasocortin-like proteins in bovine endothelial cells as well as in the bovine aortic endothelial cell line, GM 7373. Vasocortin-like proteins have been characterized by their ability to mimic the glucocorticoid inhibition of rat dextran oedema and histamine release induced by concanavalin-A in rat mast cells. Following partial purification of these proteins by gel filtration, vasocortin activity was found to be associated to proteins with molecular weight between 20-35 kD. This study showed that dexamethasone induces vasocortin-like proteins in endothelial cells and suggests that endothelial cells are target cells of glucocorticoid activity in vascular tissue.
Iuvone
T
T
Department of Experimental Pharmacology, University of Naples, Federico II, Italy.
Carnuccio
R
R
Di Rosa
M
M
D000818:Animals; D017302:Annexins; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001564:Benzo(a)pyrene / Q000494:pharmacology; D001681:Biological Assay; D002417:Cattle; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology*; D003911:Dextrans; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006636:Histamine Release / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects; D011247:Pregnancy; D014176:Protein Biosynthesis; D011506:Proteins / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D017302:Annexins; D000893:Anti-Inflammatory Agents; D003911:Dextrans; D011506:Proteins; C052038:vasocortin; D001564:Benzo(a)pyrene; D003907:Dexamethasone