Structural studies of telomeric DNA, RNA and DNA-RNA hybrid oligonucleotides by non-denaturing polyacrylamide gel electrophoresis.
Five kinds of DNA, RNA and DNA-RNA hybrid oligomers(T1-T5) containing Tetrahymena and human telomeric sequences were synthesized. T1 is d(T2G4). T2, T3, T4 and T5 are d(T2AG3), h(U2dG4), h(T2rG4) and r(U2G4) in sequences, respectively. Their structural changes were monitored by gel mobility shift on non-denaturing PAGE. The susceptibility of dG in telomeric structure was tested by methylation reaction by DMS. Our results show that telomeric DNA structure is dependent on dG to form intramolecular hydrogen bonding in the hairpin structure.
Lee
B L
BL
College of Pharmacy, Pusan National University, Korea.
Lee
K M
KM
D000818:Animals; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008745:Methylation; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000737:chemistry*; D016615:Telomere / Q000737:chemistry*; D013768:Tetrahymena / Q000235:genetics
D012313:RNA; D004247:DNA