A rapid staining procedure to demonstrate glycocalyx production and bacterial biofilms.
A novel staining procedure to demonstrate glycocalyx production by clinical isolates is presented. The short times required, specificity and sensitivity suggest that the staining could be applied to routine in vitro diagnostic procedures.
Passariello
C
C
Istituto di Microbiologia, Facoltà di Medicina, Università di Roma La Sapienza, Italy.
Berlutti
F
F
Selan
L
L
Thaller
M C
MC
Pezzi
R
R
D001422:Bacterial Adhesion; D001424:Bacterial Infections; D018441:Biofilms; D006023:Glycoproteins / Q000302:isolation & purification*; D007720:Knee Prosthesis / Q000382:microbiology; D016982:Micrococcus luteus / Q000254:growth & development; D011134:Polysaccharides / Q000302:isolation & purification*; D019736:Prostheses and Implants / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000254:growth & development; D013194:Staining and Labeling / Q000379:methods*
D006023:Glycoproteins; D011134:Polysaccharides