Sodium ion binding in the gramicidin A channel. Solid-state NMR studies of the tryptophan residues.
Gramicidin A analogs, labeled with 13C in the backbone carbonyl groups and the C-2 indole carbons of the tryptophan-11 and tryptophan-13 residues, were synthesized using t-Boc-protected amino acids. The purified analogs were incorporated into phosphatidylcholine bilayers at a 1:15 molar ratio and macroscopically aligned between glass coverslips. The orientations of the labeled groups within the channel were investigated using solid-state NMR and the effect of a monovalent ion (Na+) on the orientation of these groups determined. The presence of sodium ions did not perturb the 13C spectra of the tryptophan carbonyl groups. These results contrast with earlier results in which the Leu-10, Leu-12, and Leu-14 carbonyl groups were found to be significantly affected by the presence of sodium ions and imply that the tryptophan carbonyl groups are not directly involved in ion binding. The channel form of gramicidin A has been demonstrated to be the right-handed form of the beta 6.3 helix: consequently, the tryptophan carbonyls would be directed away from the entrance to the channel and take part in internal hydrogen bonding, so that the presence of cations in the channel would have less effect than on the outer leucine residues. Sodium ions also had no effect on the C-2 indole resonance of the tryptophan side chains. However, a small change was observed in Trp-11 when the ether lipid, ditetradecylphosphatidylcholine, was substituted for the ester lipid, dimyristoylphosphatidylcholine, indicating some sensitivity of the gramicidin side chains to the surrounding lipid.
Separovic
F
F
CRC for Molecular Engineering and Technology, CSIRO Division of Food Science and Technology, North Ryde, NSW, Australia.
Gehrmann
J
J
Milne
T
T
Cornell
B A
BA
Lin
S Y
SY
Smith
R
R
D000595:Amino Acid Sequence; D001665:Binding Sites; D002247:Carbon Isotopes; D004134:Dimyristoylphosphatidylcholine; D006096:Gramicidin / Q000737:chemistry*; D008051:Lipid Bilayers; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012964:Sodium; D014364:Tryptophan
D002247:Carbon Isotopes; D008051:Lipid Bilayers; D006096:Gramicidin; D014364:Tryptophan; D012964:Sodium; D004134:Dimyristoylphosphatidylcholine