Evolution of host cell RNA into efficient template RNA by Q beta replicase: the origin of RNA in untemplated reactions.
Q beta replicase can replicate a single molecule of certain species of RNA to 10(14) copies in minutes. This replication ability has been used for in vitro studies of molecular evolution and is currently being utilized as a method of amplifying RNAs that contain probe sequences. It has been observed that Q beta replicase can produce replicatable RNA even in the absence of exogenously added template RNA. The origin of this RNA has been ascribed either to contamination with replicatable RNA or to an ability of Q beta replicase to synthesize RNA de novo from the nucleotides present in the reaction. Technologies that employ Q beta replicase require a thorough understanding of the conditions that lead to this so-called spontaneous RNA production. We have created an expression system and purification method with which we produce gram quantities of highly purified Q beta replicase, and we have identified reaction conditions that prevent the amplification of RNA in assays that do not contain added RNA. However, when these reaction conditions are relaxed, spontaneous RNA replication is seen in up to 100% of the assays. To understand the origin of this RNA, we have cloned several spontaneously produced RNAs. Sequence analysis of one of these RNAs shows that it arose by the evolution of Escherichia coli tRNA into a replicatable template and not by de novo synthesis from nucleoside triphosphates in the reaction.
Moody
M D
MD
Gene-Trak, Incorporated, Framingham, Massachusetts 01701.
Burg
J L
JL
DiFrancesco
R
R
Lovern
D
D
Stanick
W
W
Lin-Goerke
J
J
Mahdavi
K
K
Wu
Y
Y
Farrell
M P
MP
D017908:Allolevivirus / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011777:Q beta Replicase / Q000302:isolation & purification / Q000378:metabolism*; D015347:RNA Probes; D012329:RNA, Bacterial / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D013698:Templates, Genetic
D017931:DNA Primers; D004269:DNA, Bacterial; D008670:Metals; D015347:RNA Probes; D012329:RNA, Bacterial; D012367:RNA, Viral; D012343:RNA, Transfer; D011777:Q beta Replicase