title: Induction of autologous mixed lymphocyte culture responses by myelin basic protein-reactive T cell clones.
abstract: Myelin basic protein is an autoantigen present in the central nervous system suspected to be the target of destruction in multiple sclerosis. In the present study, we have demonstrated that T cell clones specific for myelin basic protein have the ability to induce proliferative responses in resting T lymphocytes in the autologous mixed lymphocyte culture (AMLC). T cell recognition of the AMLC stimulatory determinants on the clones required the presence of autologous monocytes. T lymphocytes primed against an autologous myelin basic protein-specific T cell clone displayed specific memory responses against the original stimulating clone and failed to exhibit secondary reactivity to 'sister' myelin basic protein-reactive clones and to autologous T cell clones specific for another antigen. Monoclonal antibodies specific for class II HLA-DR antigens inhibited secondary AMLC responses. Modulation of the T cell receptor from the surface of the clones decreased their AMLC stimulatory ability. These results indicate that idiotype-like determinants on the T cell receptor of autoantigen-specific T cell clones are capable of triggering anti-idiotypic T cell responses.
authors.affiliation: Department of Neurosciences, University of Medicine & Dentistry of New Jersey, New Jersey Medical School, Newark 07103.
authors.forename: C
authors.initials: C
authors.lastname: Rohowsky-Kochan
authors.forename: D
authors.initials: D
authors.lastname: Eiman
authors.forename: T
authors.initials: T
authors.lastname: Denny
authors.forename: J
authors.initials: J
authors.lastname: Oleske
authors.forename: S D
authors.initials: SD
authors.lastname: Cook
mesh_terms: D002478:Cells, Cultured; D002999:Clone Cells; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D004676:Myelin Basic Protein / Q000276:immunology*; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000032:analysis; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D006684:HLA-DR Antigens; D004676:Myelin Basic Protein; D017260:Receptor-CD3 Complex, Antigen, T-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta