Polycos vectors: a system for packaging filamentous phage and phagemid vectors using lambda phage packaging extracts.
A new class of hybrid vectors, 'polycos' vectors, incorporate a phage lambda cos site and filamentous phage origin to allow high-efficiency cloning via in vitro lambda packaging extracts. The head-filling mechanism of cos site recognition by lambda packaging proteins permits concatemers of several of these small cos-containing vectors to be packaged per phage particle. Excision of vector monomers after infection is accomplished via a lambda ZAP-like M13 excision process. This system has the advantage of adapting high-efficiency lambda packaging extracts to M13 and phagemid cloning vectors.
Alting-Mees
M A
MA
Stratagene, La Jolla, CA 92037.
Short
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JM
D017104:Bacteriophage M13; D010582:Bacteriophage lambda; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D003360:Cosmids; D004262:DNA Restriction Enzymes; D000939:Epitopes; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins
D000939:Epitopes; D011993:Recombinant Fusion Proteins; D004262:DNA Restriction Enzymes