On the frequency of chromosome exchanges in a control population measured by chromosome painting.
Chromosome painting has been shown to be a valid and rapid method for quantifying structural chromosome rearrangements in human lymphocytes. The method is particularly useful for detecting stable aberrations which are difficult and expensive to quantify with classical methods. The inherent stability of translocations has enabled them to be used as a biodosimeter for chronic and temporally displaced exposure to radiation. Translocations may also be useful for quantifying chronic exposure to other environmental agents which may result in an accumulation of cytogenetic damage with age. Most exposures are chronic and occur at low rates, and conventional cytogenetic methods such as dicentric analysis are not expected to be informative. To understand the extent to which age and lifestyle factors impact the frequency of stable aberrations, we have performed chromosome painting on metaphase-arrested lymphocytes cultured from 47 healthy adults ranging in age from 19 to 77 years, and from umbilical cord blood obtained from eight healthy full-term infants. All subjects had previously been screened to eliminate those who had received significant occupational or accidental exposure to radiation or chemicals, and none had received chemo- or radiotherapy. Due to the infrequent occurrence of stable aberrations in peripheral lymphocytes, we analyzed the equivalent of more than 1100 metaphase cells from each of these 55 people. An average of one cell in 130 (0.77%) was observed to have a translocation or a stable insertion. A significant relationship between stable aberrations and the square of the age is apparent (R2 = 0.69, Y = 0.0615 + 0.000304 age2; p < 0.00001). These results support the hypothesis that stable aberrations accumulate with time, and are likely to integrate adverse environmental exposure.
Tucker
J D
JD
Biology and Biotechnology Research Program, L-452 Lawrence Livermore National Laboratory, Livermore, CA 94551.
Lee
D A
DA
Ramsey
M J
MJ
Briner
J
J
Olsen
L
L
Moore
D H
DH
D000328:Adult; D000367:Age Factors; D000368:Aged; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000648:ultrastructure*; D004781:Environmental Exposure; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D015321:Gene Rearrangement; D006801:Humans; D007231:Infant, Newborn; D008214:Lymphocytes / Q000166:cytology*; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D012044:Regression Analysis; D014178:Translocation, Genetic