Characterization of cDNA for a dehydration-inducible gene that encodes a CLP A, B-like protein in Arabidopsis thaliana L.
Sequence was obtained from a cDNA clone, designated ERD1, isolated from a cDNA library of 1-hour-dehydrated plants of Arabidopsis thaliana L. The clone (3150 bp) contains an open reading frame of 946 amino acid residues with greater than 34% sequence identity to the regulatory subunit of the Clp ATP-dependent protease in Escherichia coli and contains a putative chloroplast-targeting signal at the N-terminus. Southern blot analysis suggested the presence of additional ERD1-related genes in A. thaliana. The expression of ERD1 gene was strongly induced by dehydration-stress but not by heat-, cold-, or heavy-metal-stress. In addition ERD1 gene-expression was not strongly affected by treatment with plant growth regulators, such as auxin, cytokinin, abscisic acid, and gibberellic acid, or by starvation-stress for 10 hours.
Kiyosue
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T
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Yamaguchi-Shinozaki
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Shinozaki
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D049069:ATP-Dependent Proteases; D000251:Adenosine Triphosphatases; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins; D001483:Base Sequence; D060365:Chloroplast Proteins; D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis / Q000378:metabolism; D003890:Desiccation; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015723:Gene Library; D017343:Genes, Plant; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*
D029681:Arabidopsis Proteins; D060365:Chloroplast Proteins; D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D012313:RNA; D004247:DNA; D049069:ATP-Dependent Proteases; D012697:Serine Endopeptidases; D000251:Adenosine Triphosphatases; C000629270:ClpD protein, Arabidopsis