Synthetic oligonucleotides with certain palindromes stimulate interferon production of human peripheral blood lymphocytes in vitro.
We studied the ability of synthetic single-stranded 30-mer oligodeoxyribonucleotides (oligoDNAs) with three different kinds of hexamer palindromic sequence to induce interferon (IFN) production of human peripheral blood lymphocytes (PBL). When PBL was cultured with oligoDNA having a palindrome of AACGTT or GACGTC, IFN activity was detected by bioassay in the culture fluid after 8 h, and the amount of IFN reached the maximum after 18 h. IFN-alpha was predominantly produced, and small amounts of IFN-beta and IFN-gamma were also found. OligoDNA with the palindrome ACCGGT had no effect.
Yamamoto
T
T
Department of Bacterial and Blood Products, National Institute of Health, Tokyo.
Yamamoto
S
S
Kataoka
T
T
Komuro
K
K
Kohase
M
M
Tokunaga
T
T
D001483:Base Sequence; D002478:Cells, Cultured; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology*; D016898:Interferon-alpha / Q000096:biosynthesis; D016899:Interferon-beta / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D007372:Interferons / Q000096:biosynthesis*; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000494:pharmacology*; D013997:Time Factors
D007369:Interferon Inducers; D016898:Interferon-alpha; D009838:Oligodeoxyribonucleotides; D016899:Interferon-beta; D007371:Interferon-gamma; D007372:Interferons