Characterization of the 16S-23S rRNA intergenic spacer of Bartonella bacilliformis.
The 16S-23S intergenic spacer region from the ribosomal RNA (rRNA) operon of Bartonella bacilliformis was cloned and characterized. The spacer is 906 nucleotides (nt) in length and contains the genes encoding isoleucine-tRNA (tRNA(Ile)) and alanine-tRNA (tRNA(Ala)). The tRNA-encoding genes are separated by 122 nt and are centrally located in the 16S-23S spacer region, with approx. 300 flanking nt. Genes encoding tRNA(Ile) and tRNA(Ala) have 88.3 and 93.4% sequence identity, respectively, to the homologous genes of Rhodobacter sphaeroides.
Minnick
M F
MF
Division of Biological Sciences, University of Montana, Missoula 59812-1002.
Strange
J C
JC
Williams
K F
KF
D001473:Bartonella / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D009876:Operon; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D012344:RNA, Transfer, Ala / Q000235:genetics; D012355:RNA, Transfer, Ile / Q000235:genetics; D015183:Restriction Mapping; D012242:Rhodobacter sphaeroides / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D012344:RNA, Transfer, Ala; D012355:RNA, Transfer, Ile