Recent evolutionary history of American Onchocerca volvulus, based on analysis of a tandemly repeated DNA sequence family.
Polymerase chain reaction (PCR) products were characterized for a repeated sequence family (designated "O-150") of the human filarial parasite Onchocerca volvulus. In phylogenetic inferences, the O-150 sequences clustered into closely related groups, suggesting that concerted evolution maintains sequence homology in this family. Using a novel mathematical model based on a nested application of an analysis of variance, we demonstrated that African rainforest and savannah strain parasite populations are significantly different. In contrast, parasites collected in the New World are indistinguishable from African savannah strains of O. volvulus. This finding supports the hypothesis that onchocerciasis was recently introduced into the New World, possibly as a result of the slave trade.
Zimmerman
P A
PA
Department of Medicine, University of Alabama at Birmingham 35294.
Katholi
C R
CR
Wooten
M C
MC
Lang-Unnasch
N
N
Unnasch
T R
TR
D000569:Americas; D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA; D006801:Humans; D008433:Mathematics; D015233:Models, Statistical; D008969:Molecular Sequence Data; D017181:Onchocerca volvulus / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D012689:Sequence Homology, Nucleic Acid; D013698:Templates, Genetic
D004247:DNA