Rooster comb and wattle tissues contain an anti-keratan sulfate monoclonal antibody epitope.
Glycosaminoglycan fractions isolated from either comb or wattle tissue were examined using ELISA for their antigenicities to AH12, a monoclonal antibody recognizing keratan sulfate. The results showed a positive antibody binding to a sulfated glycosaminoglycan fraction from either tissue. The treatment of the same fraction with keratan sulfate degrading enzyme, keratanase, or endo-beta-galactosidase resulted in a decrease in its antigenicity. These findings indicated the presence of AH12 epitope (keratan sulfate disaccharide) in both tissues.
Nakano
T
T
Department of Animal Science, University of Alberta, Edmonton, Canada.
Sim
J S
JS
D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens / Q000276:immunology*; D003129:Comb and Wattles / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis / Q000737:chemistry*; D006595:Hexosamines / Q000032:analysis; D006820:Hyaluronic Acid / Q000032:analysis; D007632:Keratan Sulfate / Q000032:analysis*; D008297:Male
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006025:Glycosaminoglycans; D006595:Hexosamines; D006820:Hyaluronic Acid; D007632:Keratan Sulfate