title: The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen.
abstract: T-cell activation is initiated after T-cell receptor binding to antigen, but also requires interactions between costimulatory molecules expressed on antigen-presenting cells. An important costimulatory molecule expressed by monocytes and activated B lymphocytes has been recently identified and termed B7-2 or B70. Independently, a new Cluster of Differentiation was defined in the Fifth International Leukocyte Differentiation Antigen Workshop as CD86, a molecule predominantly expressed by monocytes and activated B lymphocytes. In this study, the two monoclonal antibodies that defined CD86, FUN-1 and BU-63, were shown to bind to cDNA transfected cells expressing B7-2/B70. The FUN-1 monoclonal antibody also completely blocked the costimulatory activity of B7-2/B70 in functional assays. Therefore, the serologically defined CD86 differentiation antigen is the B7-2/B70 molecule.
authors.affiliation: Department of Immunology, Duke University Medical Center, Durham, NC 27710.
authors.forename: P
authors.initials: P
authors.lastname: Engel
authors.forename: J G
authors.initials: JG
authors.lastname: Gribben
authors.forename: G J
authors.initials: GJ
authors.lastname: Freeman
authors.forename: L J
authors.initials: LJ
authors.lastname: Zhou
authors.forename: Y
authors.initials: Y
authors.lastname: Nozawa
authors.forename: M
authors.initials: M
authors.lastname: Abe
authors.forename: L M
authors.initials: LM
authors.lastname: Nadler
authors.forename: H
authors.initials: H
authors.lastname: Wakasa
authors.forename: T F
authors.initials: TF
authors.lastname: Tedder
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D001402:B-Lymphocytes / Q000276:immunology*; D018122:B7-1 Antigen / Q000032:analysis / Q000096:biosynthesis* / Q000097:blood; D051940:B7-2 Antigen; D016466:CHO Cells; D002455:Cell Division / Q000187:drug effects; D006224:Cricetinae; D000939:Epitopes / Q000032:analysis; D005455:Fluorescent Antibody Technique; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007074:Immunoglobulin G; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins; D016853:Muromonab-CD3 / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014162:Transfection
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; C497964:CD86 protein, human; D000939:Epitopes; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D016853:Muromonab-CD3; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine