title: Twenty-four-hour ice storage of rabbit heart.
abstract: Although cardioplegia is limited to 4 hours of ice storage, University of Wisconsin solution has successfully extended this period to approximately 12 hours. In this study we have substituted polyethylene glycol for hydroxyethyl starch in a simplified University of Wisconsin solution (Cardiosol). Rabbit hearts were ice stored for 24 hours at 0 degrees C in either University of Wisconsin solution or Cardiosol (containing either 5% or 10% polyethylene glycol). Fresh control hearts were tested immediately after cardiectomy. Function was evaluated in an in vitro working heart model for 1 hour with aortic afterload at 100 cm H2O. Total cardiac output or the proportion of hearts reaching 100 cm H2O were compared. Hearts stored in University of Wisconsin solution for 24 hours functioned at 6% of control levels at 15 minutes of observation. None reached 100 cm H2O or deteriorated further with time (p < 0.05). By contrast, hearts stored in 5% Cardiosol showed progressive recovery during the 1-hour observation. Of the 13 hearts, 11 reached 100 cm H2O with a mean cardiac output of 51% of the control value. Increasing the concentration of polyethylene glycol to 10% improved cardiac output at all observation times, reaching 80% of control heart performance at 1 hour (control > 10% > 5% > University of Wisconsin solution [p < 0.05]). We concluded that 10% polyethylene glycol significantly improved 24-hour ice storage and, hence, viability to a functional level that matched our previously reported microperfusion results.
authors.affiliation: Research Institute, California Pacific Medical Center, San Francisco 94115.
authors.forename: W N
authors.initials: WN
authors.lastname: Wicomb
authors.forename: D J
authors.initials: DJ
authors.lastname: Hill
authors.forename: G M
authors.initials: GM
authors.lastname: Collins
mesh_terms: D000241:Adenosine / Q000008:administration & dosage / Q000627:therapeutic use; D000493:Allopurinol / Q000008:administration & dosage / Q000627:therapeutic use; D000818:Animals; D001011:Aorta / Q000502:physiology; D001794:Blood Pressure / Q000502:physiology; D002302:Cardiac Output / Q000502:physiology; D002314:Cardioplegic Solutions / Q000008:administration & dosage / Q000627:therapeutic use*; D003326:Coronary Circulation / Q000502:physiology; D015925:Cryopreservation; D005978:Glutathione / Q000008:administration & dosage / Q000627:therapeutic use; D016027:Heart Transplantation / Q000502:physiology*; D006895:Hydroxyethyl Starch Derivatives / Q000627:therapeutic use; D007053:Ice; D007328:Insulin / Q000008:administration & dosage / Q000627:therapeutic use; D008955:Models, Cardiovascular; D015425:Myocardial Reperfusion; D019188:Organ Preservation Solutions; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000627:therapeutic use*; D011817:Rabbits; D011887:Raffinose / Q000008:administration & dosage / Q000627:therapeutic use; D013997:Time Factors; D014021:Tissue Preservation
chemical_list: D002314:Cardioplegic Solutions; D006895:Hydroxyethyl Starch Derivatives; D007053:Ice; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D011092:Polyethylene Glycols; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose