PMN adherence to cat ischemic-reperfused mesenteric vascular endothelium under flow: role of P-selectin.
We studied polymorphonuclear leukocyte (PMN) adherence to cat ischemic-reperfused mesenteric artery and vein endothelia under conditions of flow in vitro. Under physiological shear rates, only a few PMNs adhered to non-ischemic-reperfused arterial and venous endothelia (11 +/- 2 and 20 +/- 3 PMN/mm2, respectively). However, after 60 min of ischemia and 20 or 120 min of reperfusion, a significant increase in PMN adherence to arterial endothelium was observed. At 20 min of reperfusion, 44 +/- 4 PMN/mm2 adhered, and at 120 min postreperfusion, 63 +/- 12 PMN/mm2 adhered (P < 0.01 from control). Moreover, a greater degree of PMN adherence occurred on the venous than on the arterial endothelium. Thus, 159 +/- 10 and 198 +/- 12 PMN/mm2 adhered to mesenteric venous endothelium isolated after 20 and 120 min reperfusion, respectively (P < 0.01 vs. arteries). Furthermore, addition of PB 1.3 (20 micrograms/ml), a monoclonal antibody against P-selectin, 5 min before perfusion with PMNs significantly attenuated the increase in PMN adherence to both arterial and venous endothelia (P < 0.01). These results indicate that PMN-endothelial interaction also occurs in conduit vessels after ischemia and reperfusion, although a more profound PMN adherence occurs in veins.
Lefer
A M
AM
Department of Physiology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107-6799.
Ma
X L
XL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002415:Cats; D002448:Cell Adhesion / Q000502:physiology; D015816:Cell Adhesion Molecules, Neuronal / Q000276:immunology / Q000502:physiology*; D004730:Endothelium, Vascular / Q000502:physiology*; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D007511:Ischemia / Q000503:physiopathology; D008297:Male; D008638:Mesenteric Arteries / Q000502:physiology; D008642:Mesenteric Veins / Q000502:physiology; D009504:Neutrophils / Q000502:physiology*; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000276:immunology / Q000502:physiology*; D015424:Reperfusion; D013152:Splanchnic Circulation / Q000502:physiology*
D000911:Antibodies, Monoclonal; D015816:Cell Adhesion Molecules, Neuronal; D005456:Fluorescent Dyes; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins