title: Matrix-assisted laser desorption mass spectrometric analysis of a pegylated recombinant protein.
abstract: Matrix-assisted laser desorption ionization mass spectrometry (MALDI) has been investigated as a technique for the characterization of recombinant stem cell factor that had been covalently modified with polyethylene glycol (PEG) chains. The attachment of PEG chains produces a heterogeneous mixture of protein species that differ by 6000 Da. These differences in molecular weight could be readily determined by MALDI. The potential of MALDI as a general strategy for characterizing PEG-modified proteins is discussed.
authors.affiliation: Amgen, Thousand Oaks, CA.
authors.forename: E
authors.initials: E
authors.lastname: Watson
authors.forename: B
authors.initials: B
authors.lastname: Shah
authors.forename: R
authors.initials: R
authors.lastname: DePrince
authors.forename: R W
authors.initials: RW
authors.lastname: Hendren
authors.forename: R
authors.initials: R
authors.lastname: Nelson
mesh_terms: D001709:Biotechnology; D016298:Hematopoietic Cell Growth Factors / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D007834:Lasers; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D019089:Stem Cell Factor
chemical_list: D016298:Hematopoietic Cell Growth Factors; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D011092:Polyethylene Glycols