title: Translocation of an SH2-containing protein tyrosine phosphatase (SH-PTP1) to the cytoskeleton of thrombin-activated platelets.
abstract: A significant protein tyrosine phosphatase (PTP) activity was found to be associated with the cytoskeleton of thrombin-stimulated platelets. Translocation of the enzyme became maximal within 1-2 min of thrombin stimulation and was suppressed by cytochalasin D or upon inhibition of aggregation. Immunoblotting as well as immunoprecipitation revealed that a PTP with two SH2 domains (SH-PTP1) displayed the same behaviour, translocation to the cytoskeleton showing the same time course as that observed for pp60c-src. We conclude that SH-PTP1 might represent a critical enzyme in the complex interplay between the various proteins regulating protein tyrosine phosphorylation in the cytoskeletal matrix.
authors.affiliation: INSERM Unité 326, Phospholipides Membranaires, Signalisation Cellulaire et Lipoprotéines, Université Paul Sabatier, Hôpital Purpan, Toulouse, France.
authors.forename: R Y
authors.initials: RY
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Gaits
authors.forename: A
authors.initials: A
authors.lastname: Ragab
authors.forename: J M
authors.initials: JM
authors.lastname: Ragab-Thomas
authors.forename: H
authors.initials: H
authors.lastname: Chap
mesh_terms: D001692:Biological Transport; D001792:Blood Platelets / Q000187:drug effects* / Q000201:enzymology; D003599:Cytoskeleton / Q000201:enzymology*; D006801:Humans; D066298:In Vitro Techniques; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology*
chemical_list: D016392:Proto-Oncogene Proteins pp60(c-src); D017027:Protein Tyrosine Phosphatases; D013917:Thrombin