Adipogenic activities in commercial preparations of fetuin.
Fetuin is frequently in use as a serum substitute especially during the culture of differentiating preadipocyte cell lines. We show here, that the commercially available crude Pedersen fetuin contains a factor permissive for adipose conversion. It can be separated from fetuin by dialysis at acid pH. When 3T3-L1 cells are cultured in the presence of purified Pedersen fetuin, they fail to undergo adipose conversion unless this permissive adipogenic factor is added to the culture medium. Insulin-like growth factor I (IGF-I) and growth hormone (GH) are not able to replace the factor.
Bachmeier
M
M
Institut für Biochemie, Genetik und Mikrobiologie, Lehrstuhl Biochemie III Universität Regensburg, Germany.
Löffler
G
G
D017667:Adipocytes / Q000187:drug effects*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003345:Corticosterone / Q000494:pharmacology; D004340:Drug Contamination; D013006:Growth Hormone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D051379:Mice; D013234:Stem Cells / Q000187:drug effects*; D000509:alpha-Fetoproteins / Q000302:isolation & purification / Q000494:pharmacology*
D007328:Insulin; D000509:alpha-Fetoproteins; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D003345:Corticosterone