title: Detection of more than 50 different CFTR mutations in a large group of German cystic fibrosis patients.
abstract: We have conducted a comprehensive study of the molecular basis of cystic fibrosis (CF) in 350 German CF patients. A screening approach based on single-strand conformation analysis and direct sequencing of genomic polymerase chain reaction products has allowed us to detect the molecular defects on 95.4% of the CF chromosomes within the coding region and splice sites of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. The spectrum of sequence changes comprises 54 different mutations, including 17 missense mutations, 14 nonsense mutations, 11 frameshift mutations, 10 splice site variants and two amino acid deletions. Eleven of these mutations have not previously been described. Our results reflect the marked mutational heterogeneity of CF in a large sample of patients from a non-isolated population.
authors.affiliation: Klinische Forschergruppe, Molekulare Pathologie der Mukoviszidose OE 6711, Medizinische Hochschule Hannover, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Dörk
authors.forename: F
authors.initials: F
authors.lastname: Mekus
authors.forename: K
authors.initials: K
authors.lastname: Schmidt
authors.forename: J
authors.initials: J
authors.lastname: Bosshammer
authors.forename: R
authors.initials: R
authors.lastname: Fislage
authors.forename: T
authors.initials: T
authors.lastname: Heuer
authors.forename: V
authors.initials: V
authors.lastname: Dziadek
authors.forename: T
authors.initials: T
authors.lastname: Neumann
authors.forename: N
authors.initials: N
authors.lastname: Kälin
authors.forename: U
authors.initials: U
authors.lastname: Wulbrand
mesh_terms: D000328:Adult; D001483:Base Sequence; D002648:Child; D002675:Child, Preschool; D003550:Cystic Fibrosis / Q000208:ethnology / Q000235:genetics*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D004252:DNA Mutational Analysis; D005260:Female; D018740:Genetic Heterogeneity; D005820:Genetic Testing; D005858:Germany; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: C505032:CFTR protein, human; D008565:Membrane Proteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator