Regional expression of epithelial dipeptidyl peptidase IV in the human intestines.
The expression of DPP IV/CD26 was studied in epithelial cells from different regions of the human small intestine and colon. The following data were obtained: 1) DPP IV/CD26 activity was highest in ileum and jejunum, low in duodenum and not detectable in colon; 2) The amount of DPP IV, as analyzed by Western blotting, was very low in duodenum and increased up to the ileum. No DPP IV protein was detected in colon; 3) RT-PCR analysis showed the expected 315-bp product in the small intestine with the highest signal in ileum and jejunum and a low signal in duodenum; a faint PCR signal could be detected in colon. Similar data were obtained by Northern blot analysis which revealed a 3.6-kb transcript only in small intestine. It is concluded that DPP IV/CD26 gene expression in human intestine is highest in the distal small intestine, and that is regulated at the mRNA level.
Darmoul
D
D
Unité de Neuroendocrinologie et Biologie Cellulaire Digestives, INSERM U 410, Faculté de Médecine X. Bichat, Paris, France.
Voisin
T
T
Couvineau
A
A
Rouyer-Fessard
C
C
Salomon
R
R
Wang
Y
Y
Swallow
D M
DM
Laburthe
M
M
D000328:Adult; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D003106:Colon / Q000201:enzymology; D018819:Dipeptidyl Peptidase 4; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000235:genetics*; D004386:Duodenum / Q000201:enzymology; D004848:Epithelium / Q000201:enzymology; D015870:Gene Expression; D006801:Humans; D007082:Ileum / Q000201:enzymology; D007422:Intestines / Q000201:enzymology*; D007583:Jejunum / Q000201:enzymology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase
D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases; D018819:Dipeptidyl Peptidase 4