title: Induction of interleukin-2 receptor alpha chain expression of immature acute myelocytic leukemia cells.
abstract: Leukemic cells from 27 adult patients with acute myelocytic leukemia (AML) were investigated to determine the cell surface inducibility of interleukin-2 receptor (IL-2R) in in vitro culture with and without interleukin-1 beta (IL-1 beta). IL-2R alpha chain (IL-2R alpha) was induced on leukemic cells in 11 of 27 cases. Most of the cases could induce IL-2R alpha spontaneously without IL-1 beta, while the IL-2R beta chain (IL-2R beta) did not appear on leukemic cells from any of the cases tested. AML cases expressing CD7 or HLA-DR antigen could induce IL-2R alpha more frequently than any other type of AML. Among interleukin-3 (IL-3), granulocyte-macrophage colony stimulating factor (GM-CSF) and G-CSF, IL-3 showed a more prominent effect on DNA synthesis in IL-2R alpha inducible cases than in its uninducible cases. These results suggest that IL-2R alpha but not IL-2R beta was easily inducible on AML cells with immature characteristics.
authors.affiliation: Department of Internal Medicine, Yamada Red Cross Hospital, Misono, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nakase
authors.forename: K
authors.initials: K
authors.lastname: Kita
authors.forename: H
authors.initials: H
authors.lastname: Anazawa
authors.forename: K
authors.initials: K
authors.lastname: Hoshino
authors.forename: S
authors.initials: S
authors.lastname: Shirakawa
authors.forename: I
authors.initials: I
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Tsudo
mesh_terms: D000208:Acute Disease; D000328:Adult; D002455:Cell Division; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D016130:Immunophenotyping; D066298:In Vitro Techniques; D007375:Interleukin-1 / Q000494:pharmacology; D007377:Interleukin-3 / Q000494:pharmacology; D007951:Leukemia, Myeloid / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D015375:Receptors, Interleukin-2 / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
chemical_list: D007375:Interleukin-1; D007377:Interleukin-3; D015375:Receptors, Interleukin-2; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor