Analysis of benzo[a]pyrene induced mutations by the use of Restriction-Site Mutation assays in aquatic species.
Larval and toadlet stages of the clawed toad Xenopus laevis were exposed to benzo[a]pyrene in aquatic media. Mutations were analysed in a variety of restriction enzyme recognition sequences of the adult alpha 1-globin gene. Mutations were detected in the BsiLi recognition site (CCTGG) at early sampling times (6 h) in larval stages and at 8-days sampling time in post-metamorphosis toadlets. The predominant mutation detected was a G-->T base transversion at the 5th base of the CCTGG sequence. The data presented indicates that the Restriction-Site Mutation methodology has considerable potential for development as a technique for monitoring the genotoxic potential of water-borne toxins.
Kennerley
G A
GA
School of Biological Sciences, University of Wales, Swansea, Singleton Park, UK.
Parry
J M
JM
D000818:Animals; D001483:Base Sequence; D001564:Benzo(a)pyrene / Q000633:toxicity*; D004247:DNA; D005240:Feasibility Studies; D008969:Molecular Sequence Data; D009152:Mutagenicity Tests / Q000379:methods*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014867:Water; D014982:Xenopus laevis
D014867:Water; D001564:Benzo(a)pyrene; D004247:DNA