The presence of CD5LOW+NK cells in normal controls and patients with pulmonary tuberculosis.
CD5 antigen is present on all normal alpha beta T cells and some B cells. Human NK cells do not usually express CD5 antigen, but we found a subset of CD5LOW+ (low density of CD5) NK cells in some patients with pulmonary tuberculosis. Unlike CD5-NK cells, most CD5LOW+NK cells had HLA-DR. We observed few CD5LOW+NK cells in the normal controls and some in the large granular lymphocyte (LGL) population purified by Percoll density centrifugation. Sorted CD5LOW+NK populations were LGL. The CD5LOW+NK cells had high lytic activity on K562 cells in a 4-h 51chromium release assay. Our results indicate that there is a previously unidentified subset of NK cells.
Ishiyama
T
T
Department of Hematology, Showa University School of Medicine, Tokyo, Japan.
Watanabe
K
K
Fukuchi
K
K
Yajima
K
K
Koike
M
M
Tomoyasu
S
S
Tsuruoka
N
N
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis*; D018956:CD5 Antigens; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology; D008875:Middle Aged; D017452:Receptors, IgG / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
D015703:Antigens, CD; D018956:CD5 Antigens; D006684:HLA-DR Antigens; D017452:Receptors, IgG