Effect of recombinant human colony-stimulating factor on the course of parasitaemia in non-lethal rodent malaria.
The effect of repeated subcutaneous injections of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on the attenuated Plasmodium berghei XAT infection in CBA mice was examined. When mice were injected with rhG-CSF daily beginning 2 days before infection, the neutrophil count in the peripheral blood increased 5 times higher than that of control mice and the development of parasitaemia was suppressed significantly during the early phase of the infection. This suppressive effect of rhG-CSF was reduced by treatment of the mice with either anti-interferon (IFN)-gamma or anti-tumour necrosis factor (TNF)-alpha immunoglobulins. These results suggest that neutrophils may have a role in immunity against the parasites and that IFN-gamma and TNF-alpha are possibly involved.
Waki
S
S
Department of Parasitology, Gunma University School of Medicine, Maebashi, Japan.
Kurihara
R
R
Nemoto
H
H
Suzuki
M
M
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000187:drug effects*; D007279:Injections, Subcutaneous; D007371:Interferon-gamma / Q000276:immunology; D008288:Malaria / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D010962:Plasmodium berghei / Q000187:drug effects*; D011994:Recombinant Proteins / Q000187:drug effects; D012376:Rodent Diseases / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D000906:Antibodies; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D016179:Granulocyte Colony-Stimulating Factor; D007371:Interferon-gamma