[A method for preparing a monolayer of frog brain cells for the cytophotometric determination of their DNA content].
An improved method for the squashing of the brain cells permitting a good preservation of cell nuclei and their subsequent quantitative cytophotometric evaluation is presented. By this method a monolayer of the brain cells can be easily obtained. The method was tested in cytophotometric study of the DNA content in the Feulgen stained cells of the frog hypothalamic preoptic area. The proposed method can be used for the quantitative evaluation of different histochemical reactions in the brain cells.
Onishchenko
L S
LS
Chetverukhin
V K
VK
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D001923:Brain Chemistry; D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure; D002478:Cells, Cultured / Q000737:chemistry / Q000166:cytology; D004396:Coloring Agents; D003584:Cytological Techniques; D003592:Cytophotometry / Q000379:methods*; D004247:DNA / Q000032:analysis*; D005260:Female; D008297:Male; D009474:Neurons / Q000737:chemistry / Q000648:ultrastructure; D011301:Preoptic Area / Q000737:chemistry / Q000648:ultrastructure; D011896:Rana temporaria; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D004247:DNA