Vascular adhesion molecule expression in viral chronic hepatitis: evidence of neoangiogenesis in portal tracts.
OBJECTIVE
T cell-mediated immune reactions could be crucial for hepatocellular damage in viral chronic hepatitis. The aims of this study were to compare the expression of activation and cell adhesion molecules on peripheral blood and intrahepatic lymphocytes from chronic hepatitis C and to analyze the intrahepatic expression of vascular adhesion molecules in viral chronic hepatitis.


METHODS
Lymphocytes from patients with chronic hepatitis C were studied by flow cytometry. Intrahepatic expression of vascular adhesion molecules was assessed by immunohistochemistry.


RESULTS
Liver-derived T cells showed a high expression of activation and cell adhesion molecules. Interestingly, we observed that vascular cell adhesion molecule 1 was up-regulated on both sinusoidal endothelial and portal dendritic cells. A novel finding was the neoformation of microvessels in inflamed portal tracts. An enhanced expression of endoglin was located on sinusoidal endothelial cells and on portal tracts.


CONCLUSIONS
Activated cytotoxic T cells, which showed an up-regulated expression of cell adhesion molecules, composed the majority of intrahepatic lymphocytes in chronic hepatitis C. The expression of vascular cell adhesion molecule 1 on portal dendritic cells and the microvessels neoformation in portal tracts from viral chronic hepatitis could define the main pathway for the recruitment and priming of liver-infiltrating T cells.
García-Monzón
C
C
Liver Unit, Hospital de la Princesa, Universidad Autónoma de Madrid, Spain.
Sánchez-Madrid
F
F
García-Buey
L
L
García-Arroyo
A
A
García-Sánchez
A
A
Moreno-Otero
R
R
D001773:Blood Cells / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002908:Chronic Disease; D005434:Flow Cytometry; D006525:Hepatitis, Viral, Human / Q000150:complications* / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008213:Lymphocyte Activation; D009389:Neovascularization, Pathologic / Q000209:etiology*; D011168:Portal System; D013601:T-Lymphocytes / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1
D015815:Cell Adhesion Molecules; D019010:Vascular Cell Adhesion Molecule-1