Expression of the VP3-VP1 sequence of foot-and-mouth disease virus in Escherichia coli.
1. cDNA recombinants containing the VP3 and VP1 sequences of foot-and-mouth disease virus were isolated and the VP3-VP1 sequence was reconstructed. 2. The reconstructed VP3-VP1 sequence was subcloned into expression vector pEX31b and a fusion protein of about 62,000 Da was expressed. 3. When injected into mice, the fusion protein was able to elicit the production of antibodies that recognized viral VP1 and VP3. 4. Antibodies present in sera from mice immunized with VP3-VP1 protein did not neutralize the foot-and-mouth disease virus in vitro.
Rossetti
M L
ML
Departamento de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
Raupp
R M
RM
Farias
S E
SE
Ferreira
L F
LF
Zaha
A
A
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D005537:Aphthovirus / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D002213:Capsid / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D036022:Capsid Proteins; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics*; D051379:Mice; D009500:Neutralization Tests; D014760:Viral Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral; D036022:Capsid Proteins; D000939:Epitopes; D014760:Viral Fusion Proteins