Simple device for continuous measurement of fluorescent anions and cations in the rat kidney in situ.
A simple device for fluorescence measurements on kidney surface in situ (FKS) is described. The device consists of (1) an illuminating unit, (2) a detector, (3) an evaluation unit. The device was developed for measurement of tissue content of anionic sulfofluorescein and cationic 4(4-dimethylaminostyryl)-N-methylpyridinium (ASP) during its secretion by proximal renal tubular cells.
Rohlicek
V
V
Institute of Physiology AVCR, Prague, Czech Republic.
Ullrich
K J
KJ
D000818:Animals; D000838:Anions / Q000032:analysis*; D002412:Cations / Q000032:analysis*; D005456:Fluorescent Dyes / Q000032:analysis; D005470:Fluorometry / Q000295:instrumentation; D007668:Kidney / Q000737:chemistry* / Q000378:metabolism; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D011726:Pyridinium Compounds / Q000032:analysis; D051381:Rats; D010130:p-Aminohippuric Acid / Q000032:analysis
C032315:4-(4-dimethylaminostyryl)-1-methylpyridinium; D000838:Anions; D002412:Cations; D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; D010130:p-Aminohippuric Acid