ColE1 multimer formation triggers inhibition of Escherichia coli cell division.
Multimer formation and consequent copy number depression are acknowledged causes of multicopy plasmid instability. Multimer resolution sites (among which ColE1 cer is best-characterized) have been identified in a variety of plasmids. They participate in the conversion of multimers to monomers, maximizing the number of independently segregating molecules and minimizing the frequency of plasmid loss. We show that multimer resolution alone is insufficient to ensure stable maintenance of ColE1-like plasmids in a recombination-proficient host. The expression of Rcd, a transcript encoded within cer and expressed in multimer-containing cells, is also required. The appearance of Rcd correlates with the inhibition of division of multimer-containing cells, presumably allowing time for the conversion of multimers to monomers by site-specific recombination.
Patient
M E
ME
Department of Genetics, Cambridge, UK.
Summers
D K
DK
D001426:Bacterial Proteins / Q000096:biosynthesis*; D001483:Base Sequence; D015152:Blotting, Northern; D002455:Cell Division; D003087:Colicins / Q000037:antagonists & inhibitors*; D003198:Computer Simulation; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015345:Oligonucleotide Probes; D009876:Operon; D010957:Plasmids; D012329:RNA, Bacterial / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D013997:Time Factors; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D003087:Colicins; D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012333:RNA, Messenger; C007104:colicin immunity proteins