title: Enzymatic synthesis of milligram quantities of ribozymes in small volumes.
abstract: Milligram quantities of ribozymes (Rzs) can be synthesized in vitro in reaction volumes of 1 mL or less using AmpliScribe T7 RNA polymerase kits to transcribe either linear plasmids or oligodeoxynucleotide templates. Model hammerhead Rzs were synthesized that specifically cleave RNA-encoding chloramphenicol acetyl transferase sequences. Methods are presented for the transcription of Rzs of virtually any length and sequence composition at a fraction of the cost of chemical synthesis.
authors.affiliation: Epicentre Technologies, Madison, WI.
authors.forename: L M
authors.initials: LM
authors.lastname: Hoffman
authors.forename: M G
authors.initials: MG
authors.lastname: Johnson
mesh_terms: D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D008969:Molecular Sequence Data; D012313:RNA / Q000378:metabolism; D016337:RNA, Catalytic / Q000096:biosynthesis*; D011933:Reagent Kits, Diagnostic; D014158:Transcription, Genetic
chemical_list: D016337:RNA, Catalytic; D011933:Reagent Kits, Diagnostic; D012313:RNA; D015500:Chloramphenicol O-Acetyltransferase