A cationic protein from a urate-calcium oxalate stone: isolation and purification of a shared protein.
A protein extracted from a urate-calcium oxalate stone by electrodialysis is also excreted in the urine which served as the source material for its purification by FPLC after separation on an ACA44 column. It has an amino acid composition appropriate for a cationic protein. One peptide obtained by cyanogen bromide cleavage has significant (approximately 60%) homology with CD59 protein (protectin). Both proteins have wide distribution, the unknown having been found in bile, cholesterol gallstones, and the wall of the aorta. However, the two proteins appear to be immunologically different.
Binette
J P
JP
Veterans Administration Medical Center, Buffalo, NY 14215.
Binette
M B
MB
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000737:chemistry / Q000276:immunology; D018957:CD59 Antigens; D002129:Calcium Oxalate / Q000032:analysis*; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007473:Ion Channels / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000652:urine; D007526:Isoelectric Point; D007669:Kidney Calculi / Q000737:chemistry*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014527:Uric Acid / Q000032:analysis*
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018957:CD59 Antigens; D007473:Ion Channels; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; C086481:cationic protein, urate-calcium oxalate stone, human; D002129:Calcium Oxalate; D014527:Uric Acid