Expression and analysis of the NS2 protein of influenza A virus.
Influenza NS2 protein was expressed in Saccharomyces cerevisiae using a copper-inducible promoter. The protein produced had a molecular weight of 13 kDa, was reactive with anti-NS2 antiserum and was localised to the yeast cell nucleus. Two-hybrid analysis identified a direct protein-protein interaction between NS2 and the M2 protein of the virus, involving the C-terminal 163 residues of M1. A filter-binding assay localised the M1 binding region to the C-terminal 70 amino acids of NS2.
Ward
A C
AC
Biomolecular Research Institute, Parkville, Victoria, Australia.
Castelli
L A
LA
Lucantoni
A C
AC
White
J F
JF
Azad
A A
AA
Macreadie
I G
IG
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D009980:Influenza A virus / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae; D014763:Viral Matrix Proteins / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D000086082:Viroporin Proteins
D017931:DNA Primers; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins; C031540:M-protein, influenza virus; C488936:M1 protein, Influenza A virus; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins