Thrombolytic activity of YM866, a novel modified tissue-type plasminogen activator, in a photochemically induced platelet-rich thrombosis model.
We compared the thrombolytic activity of a novel modified tissue-type plasminogen activator (t-PA; del 92-173, 275Arg-->Glu), YM866, with that of t-PA in a platelet-rich thrombosis model. Thrombus was induced in guinea pig mesenteric artery by irradiation with filtered light in combination with intravenous (i.v.) administration of fluorescent dye. When occlusion by the thrombus extended to 99% of the luminal area of the vessel, test drug (YM866, t-PA, or saline) was administered by i.v. bolus injection under heparinization. Both YM866 and t-PA exhibited dose-dependent thrombolytic activity; however, the improvement in occlusion rate and the incidence of successful thrombolysis induced by YM866 were three times higher than those induced by t-PA. With YM866 1 mg/kg, alpha 2-plasmin inhibitor levels decreased significantly to 58% of saline group values, but no change was noted in fibrinogen levels. YM866 antigen levels at this dose were seven times higher than those of t-PA. These results suggest that YM866 in single bolus injection is a thrombolytic agent superior to t-PA in platelet-rich thrombi without systemic fibrinolytic activation and that this efficacy is due to the prolonged half-life (t1/2) of the drug.
Kawasaki
T
T
Cardiovascular Laboratory, Yamanouchi Pharmaceutical, Ibaraki, Japan.
Kaku
S
S
Takenaka
T
T
Yanagi
K
K
Ohshima
N
N
D000818:Animals; D000941:Antigens / Q000097:blood; D001792:Blood Platelets / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D005342:Fibrinolysis / Q000187:drug effects; D006168:Guinea Pigs; D010777:Photochemistry; D010974:Platelet Aggregation / Q000187:drug effects / Q000528:radiation effects; D010976:Platelet Count / Q000187:drug effects; D011994:Recombinant Proteins / Q000097:blood / Q000494:pharmacology; D013927:Thrombosis / Q000188:drug therapy* / Q000209:etiology / Q000473:pathology; D010959:Tissue Plasminogen Activator / Q000097:blood / Q000494:pharmacology*
D000941:Antigens; D011994:Recombinant Proteins; D010959:Tissue Plasminogen Activator; C085027:pamiteplase