Rilmenidine-induced hypotension in conscious rabbits involves imidazoline-preferring receptors.
The relative contributions of imidazoline-preferring receptors (IPR) and alpha 2-adrenoceptors to hypotensive and bradycardic effects of intracisternal (i.c.) rilmenidine were investigated in conscious rabbits. We compared the antagonist potencies of two alpha 2-adrenoceptor antagonists, 2-methoxy-idazoxan (0.001-10 micrograms/kg i.c.), which has very low affinity for IPR, and idazoxan (0.003-30 micrograms/kg i.c.), which has high affinity for blocking IPR. We also compared the i.c. effects of the antagonists on responses to alpha-methyldopa (alpha-MD), a drug with centrally acting alpha 2-adrenoceptor agonist metabolites that have no affinity for IPR. Rilmenidine (22 micrograms/kg i.c.) and alpha-MD (400 micrograms/kg i.c.) produced similar decreases in mean arterial pressure (MAP) (delta MAP = -23 +/- 2 and -24 +/- 2%, respectively) and in heart rate (HR) (delta HR = -11 +/- 1 and -9 +/- 2%, respectively, n = 30). The hypotension and bradycardia produced by alpha-MD and rilmenidine were completely reversed by 2-methoxy-idazoxan, but 2-methoxy-idazoxan was 16 and 9 times more potent at restoring MAP and HR, respectively, after alpha-MD than after rilmenidine. In contrast, idazoxan was more potent in reversing the hypotension elicited by i.c. injections of rilmenidine than that elicited by alpha-MD. Idazoxan, however, had no effect on rilmenidine-induced bradycardia, but did dose-dependently reverse the decrease in HR produced by alpha-MD. In separate experiments, we observed that the doses of each antagonist drug in themselves did not modify MAP nor HR significantly, but a 10-fold higher dose of idazoxan (300 micrograms/kg) caused immediate although brief hypertension and tachycardia.(ABSTRACT TRUNCATED AT 250 WORDS)
Sannajust
F
F
Baker Medical Research Institute, Department of Neuropharmacology, Prahran, Victoria, Australia.
Head
G A
GA
D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects*; D004146:Dioxanes / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D005260:Female; D006339:Heart Rate / Q000187:drug effects*; D007022:Hypotension / Q000139:chemically induced; D019329:Idazoxan / Q000031:analogs & derivatives; D054755:Imidazoline Receptors; D008297:Male; D008750:Methyldopa / Q000494:pharmacology; D010080:Oxazoles / Q000008:administration & dosage / Q000494:pharmacology*; D011817:Rabbits; D011955:Receptors, Drug / Q000187:drug effects* / Q000378:metabolism; D000077769:Rilmenidine
D000317:Adrenergic alpha-Antagonists; D000959:Antihypertensive Agents; D004146:Dioxanes; D054755:Imidazoline Receptors; D010080:Oxazoles; D011955:Receptors, Drug; D008750:Methyldopa; C058525:2-methoxyidazoxan; D000077769:Rilmenidine; D019329:Idazoxan