title: DNA variation in the genes of the Na,K-adenosine triphosphatase and its relation with resting metabolic rate, respiratory quotient, and body fat.
abstract: The aim of this study was to investigate in 261 subjects from 58 families the association between DNA variation at the genes coding for the Na,K-ATPase peptides and resting metabolic rate (RMR), respiratory quotient (RQ), and percent body fat (%FAT). Five restriction fragment length polymorphisms (RFLP) at three Na,K-ATPase genes were determined: one at the alpha 1 locus (BglII), and two at the beta locus (beta MspI and beta PvuII). Haplotypes were determined from the two variable sites of the alpha 2 gene (alpha 2 haplotypes) and the beta gene (beta haplotypes). There was a strong trend for %FAT to be related to the RFLP generated by BglII at the alpha 2 exons 21-22 in males (P = 0.06) and females (P = 0.05). RQ was (a) associated with the BglII RFLP at the alpha 2 exon 1 (P = 0.02) and with the alpha 2 8.0 kb/4.3 kb haplotype (P = 0.04) and (b) linked with the beta gene MspI marker (P = 0.04) and with the beta 5.3 kb/5.1 kb haplotype (P = 0.008) based on sib-pair analysis. The present study suggests that the genes encoding Na,K-ATPase may be associated or linked with RQ and perhaps with %FAT but not with RMR.
authors.affiliation: Physical Activity Sciences Laboratory, Laval University, Ste-Foy, Québec, Canada.
authors.forename: O
authors.initials: O
authors.lastname: Dériaz
authors.forename: F
authors.initials: F
authors.lastname: Dionne
authors.forename: L
authors.initials: L
authors.lastname: Pérusse
authors.forename: A
authors.initials: A
authors.lastname: Tremblay
authors.forename: M C
authors.initials: MC
authors.lastname: Vohl
authors.forename: G
authors.initials: G
authors.lastname: Côté
authors.forename: C
authors.initials: C
authors.lastname: Bouchard
mesh_terms: D000273:Adipose Tissue / Q000033:anatomy & histology*; D000328:Adult; D000367:Age Factors; D000483:Alleles; D000704:Analysis of Variance; D001426:Bacterial Proteins; D001481:Basal Metabolism; D015342:DNA Probes; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D005091:Exons; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D010101:Oxygen Consumption; D012150:Polymorphism, Restriction Fragment Length; D012737:Sex Factors; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D015342:DNA Probes; D019003:Deoxyribonuclease HpaII; C024404:BglII endonuclease; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D000254:Sodium-Potassium-Exchanging ATPase