Excision of chromosomal DNA loops by treatment of permeabilised cells with Bal 31 nuclease.
The specificity of long-range fragmentation of human nucleolar genes by Bal 31 nuclease was studied using fractionation of cleavage products by pulsed field gel electrophoresis, followed by Southern hybridization. It was found that limited treatment of permeabilised cells with this nuclease results in accumulation of DNA scissions in matrix attachment areas. Consequently, chromosomal DNA loops and their oligomers are released from the genome.
Lagarkova
M A
MA
Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Iarovaia
O V
OV
Razin
S V
SV
D002460:Cell Line; D002463:Cell Membrane Permeability; D002466:Cell Nucleolus / Q000378:metabolism; D002877:Chromosomes, Human; D004273:DNA, Neoplasm / Q000302:isolation & purification / Q000378:metabolism*; D004275:DNA, Ribosomal / Q000302:isolation & purification / Q000378:metabolism*; D004706:Endodeoxyribonucleases / Q000378:metabolism*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D004275:DNA, Ribosomal; D004706:Endodeoxyribonucleases; C038407:exonuclease Bal 31