title: Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies.
abstract: Monoclonal antibodies (MAbs) to two U.S. isolates of porcine reproductive and respiratory syndrome (PRRS) virus were prepared. Two MAbs specifically recognized a conserved epitope on the putative 15-kDa nucleocapsid protein of U.S. and European isolates of PRRS virus. Four other MAbs recognized epitopes on the 15-kDa protein of U.S. but not European isolates of PRRS virus. Collectively, this indicates that PRRS viruses contain both conserved and divergent epitopes on the 15-kDa viral protein.
authors.affiliation: Department of Veterinary Science, South Dakota State University, Brookings 57007.
authors.forename: E A
authors.initials: EA
authors.lastname: Nelson
authors.forename: J
authors.initials: J
authors.lastname: Christopher-Hennings
authors.forename: T
authors.initials: T
authors.lastname: Drew
authors.forename: G
authors.initials: G
authors.lastname: Wensvoort
authors.forename: J E
authors.initials: JE
authors.lastname: Collins
authors.forename: D A
authors.initials: DA
authors.lastname: Benfield
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000302:isolation & purification; D018018:Arterivirus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification*; D000939:Epitopes / Q000302:isolation & purification; D005060:Europe; D005260:Female; D005831:Genital Diseases, Female / Q000382:microbiology / Q000662:veterinary*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014481:United States; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D014764:Viral Proteins