Neostriatal GABAergic interneurones contain NOS, calretinin or parvalbumin.
To reveal functional heterogeneity among GABAergic interneurones in rat neostriatum, we investigated: (1) whether nitric oxide synthase (NOS)- and calretinin-immunoreactive cells, like parvalbumin-immunoreactive cells, were also immunoreactive for 67 kD glutamic acid decarboxylase (GAD67) and GABA; (2) whether NOS cells, calretinin cells and parvalbumin cells belonged to separate populations or not. NOS cells, calretinin cells and parvalbumin cells all showed immunoreactivity for GAD67 in colchicine-treated rats. Calretinin cells and parvalbumin cells were also immunoreactive for GABA. Only a few cells (0-3%) showed immunoreactivity for two antigens. These results suggest that the neostriatum has at least three GABAergic interneuronal systems.
Kubota
Y
Y
Laboratory for Neural Systems, RIKEN, Wako, Japan.
Mikawa
S
S
Kawaguchi
Y
Y
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D064032:Calbindin 2; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D007395:Interneurons / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D008297:Male; D017072:Neostriatum / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D019001:Nitric Oxide Synthase; D010320:Parvalbumins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D064030:S100 Calcium Binding Protein G / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
C576512:Calb2 protein, rat; D064032:Calbindin 2; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; D005680:gamma-Aminobutyric Acid; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases