Solid basal cell epithelioma (BCE) possibly originates from the outer root sheath of the hair follicle.
The presence and distribution of several cytokeratins (CKs) in 20 solid basal cell epitheliomas (BCEs) were investigated and compared with the pattern of CKs in normal epidermis, perilesional skin and in the outer root sheath (ORS) of the human hair follicle. Tissue samples were stained with monoclonal antibodies (MoAbs) against human CKs, using the APAAP technique. Additionally, CK profiles were assessed by gel electrophoresis and immunoblot technique. Cells of BCE and ORS were positively stained with the MoAb KL1, whereas the basal layer of normal epidermis remained negative. Six out of 20 BCEs were partially stained with the MoAb RPN1165, which also stained the lower ORS, but not the epidermal basal layer. Using SDS-PAGE and immunoblot, the CK profiles of BCE and ORS were almost identical, showing the presence of CKs 5, 6, 14, 16 and 17; the CK pattern of normal epidermis, however, showed the presence of CKs 1, 5, 10 and 14. Perilesional skin (< 5 mm) showed keratin changes similar to the BCE pattern; the basal layer was stained with the MoAb KL1 and the suprabasal layer was negative to MoAb CK1, in contrast to normal epidermis. Keratin analysis revealed a CK pattern of perilesional skin different from that of normal epidermis (CKs 1, 5, 6, 10, 14, 16 and 17). Our immunohistochemical and biochemical investigations underline the possible role of the lower ORS as a cellular pool for the generation of BCE.
Asada
M
M
Department of Dermatology, University Medical Center Steglitz, Free University of Berlin, Germany.
Schaart
F M
FM
de Almeida
H L
HL
Korge
B
B
Kurokawa
I
I
Asada
Y
Y
Orfanos
C E
CE
D000911:Antibodies, Monoclonal; D002280:Basal Cell Carcinoma / Q000737:chemistry* / Q000473:pathology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006197:Hair / Q000737:chemistry* / Q000166:cytology; D006801:Humans; D007633:Keratins / Q000032:analysis*; D012535:Scalp; D012878:Skin Neoplasms / Q000737:chemistry* / Q000473:pathology; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D007633:Keratins