title: Separation of origin recognition complex functions by cross-species complementation.
abstract: Transcriptional silencing at the HMRa locus of Saccharomyces cerevisiae requires the function of the origin recognition complex (ORC), the replication initiator of yeast. Expression of a Drosophila melanogaster Orc2 complementary DNA in the yeast orc2-1 strain, which is defective for replication and silencing, complemented the silencing defect but not the replication defect; this result indicated that the replication and silencing functions of ORC were separable. The orc2-1 mutation mapped to the region of greatest homology between the Drosophila and yeast proteins. The silent state mediated by DmOrc2 was epigenetic; it was propagated during mitotic divisions in a relatively stable way, whereas the nonsilent state was metastable. In contrast, the silent state was erased during meiosis.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
authors.forename: A E
authors.initials: AE
authors.lastname: Ehrenhofer-Murray
authors.forename: M
authors.initials: M
authors.lastname: Gossen
authors.forename: D T
authors.initials: DT
authors.lastname: Pak
authors.forename: M R
authors.initials: MR
authors.lastname: Botchan
authors.forename: J
authors.initials: J
authors.lastname: Rine
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology; D005786:Gene Expression Regulation; D005800:Genes, Fungal; D017344:Genes, Insect; D005816:Genetic Complementation Test; D009154:Mutation; D051738:Origin Recognition Complex; D012045:Regulatory Sequences, Nucleic Acid; D018741:Replication Origin; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature; D014170:Transformation, Genetic
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005656:Fungal Proteins; C497215:ORC2 protein, S cerevisiae; C497216:Orc2 protein, Drosophila; D051738:Origin Recognition Complex; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins