Production of human cloned antibodies specific for hepatitis D virus-encoded small and large protein.
Cloned antibodies to specific epitopes of hepatitis D virus were produced by transformation with Epstein-Barr virus and subsequent cloning of peripheral blood B lymphocytes from a patient with chronic hepatitis D virus infection. Several stable cloned B cell lines, derived from two parent cultures, produced hepatitis D-virus-specific IgG antibodies. Some cloned IgG antibodies detected hepatitis D virus-associated antigen in hepatitis D virus-infected woodchuck liver tissue sections by indirect immunofluorescence staining and some reacted in an inhibition ELISA test detecting hepatitis D virus antibodies; most cloned IgG lines detected hepatitis D antigen both in immunofluorescence tests and in inhibition ELISA. Cloned antibodies to hepatitis D antigen detected by ELISA and/or immunofluorescence staining recognized the two major specific native and denatured polypeptides, p27 and p29, in Western blot analysis. Such cloned antibodies for hepatitis D virus are potentially useful for clinical diagnosis and research.
Liu
F
F
Max v. Pettenkofer-Institute for Hygiene and Medical Microbiology, Munich, Germany.
Roggendorf
M
M
Rasshofer
R
R
Zachoval
R
R
Deinhardt
F
F
D000914:Antibodies, Viral / Q000276:immunology* / Q000378:metabolism; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D015153:Blotting, Western; D002461:Cell Line, Transformed; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D003699:Hepatitis D / Q000473:pathology; D003698:Hepatitis Delta Virus / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D008099:Liver / Q000737:chemistry; D014759:Viral Envelope Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D007074:Immunoglobulin G; D014759:Viral Envelope Proteins