title: Physical mapping of the human hprt chromosomal region (Xq26).
abstract: The human hprt chromosomal region (Xq26) was physical-mapped using pulsed field gel electrophoresis (PFGE). This work involved: (i) the recovery of three new genomic DNA markers (DXS1327, DXS1328, and DXS1329), (ii) the ordering of new markers relative to 11 previously available hprt-linked markers by deletion mapping, and (iii) the completion of human T-lymphocyte PFGE Southern blots using the 14 Xq26 markers. A contiguous 1.5-Mb physical map of the region telomeric to hprt was determined. As this map identifies clusters of in vivo unmethylated rare-cutter restriction sites, potential CpG islands are revealed.
authors.affiliation: University of Vermont, VCC Genetics Laboratory, Burlington 05401.
authors.forename: M J
authors.initials: MJ
authors.lastname: Lippert
authors.forename: R J
authors.initials: RJ
authors.lastname: Albertini
authors.forename: J A
authors.initials: JA
authors.lastname: Nicklas
mesh_terms: D000328:Adult; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D015342:DNA Probes; D004262:DNA Restriction Enzymes; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D008214:Lymphocytes; D008297:Male; D014960:X Chromosome
chemical_list: D015342:DNA Probes; D007041:Hypoxanthine Phosphoribosyltransferase; D004262:DNA Restriction Enzymes