Cloning and expression kinetics of porcine vascular cell adhesion molecule.
Human vascular cell adhesion molecule is believed to play a key role in recruiting leukocytes to sites of injury. Here we report the identification of a homologous molecule in the pig which has five Ig domains and an overall 77% homology with the human protein. The expression of this protein was also characterised on porcine endothelial cells. Like the human adhesion molecule, the porcine protein could be induced by LPS, TNF and IL-1 alpha, although differences were noted in the kinetics of expression.
Tsang
Y T
YT
Celltech Research, Celltech Ltd., Slough, UK.
Haskard
D O
DO
Robinson
M K
MK
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice / Q000276:immunology; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins; D019010:Vascular Cell Adhesion Molecule-1; D014682:Vena Cava, Inferior
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D007375:Interleukin-1; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1