title: Co-existence of PACAP and nitric oxide synthase in the rat hypothalamus.
abstract: The possible co-existence of pituitary adenylate cyclase-activating polypeptide (PACAP)-38 and nitric oxide synthase (NOS) in the rat hypothalamus was examined by a combination of PACAP-immunocytochemistry and NADPH-diaphorase histochemistry. Virtually all PACAP-38-immunoreactive neurones in the paraventricular and supraoptic nuclei exhibited NADPH-diaphorase activity. Since NADPH-diaphorase activity was identical to NOS-immunoreactivity in the magnocellular neurosecretory neurones, this finding indicates that the PACAP neurones synthesize NO.
authors.affiliation: Department of Anatomy, Kyoto Prefectural University of Medicine, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Okamura
authors.forename: A
authors.initials: A
authors.lastname: Miyagawa
authors.forename: H
authors.initials: H
authors.lastname: Takagi
authors.forename: H
authors.initials: H
authors.lastname: Esumi
authors.forename: N
authors.initials: N
authors.lastname: Yanaihara
authors.forename: Y
authors.initials: Y
authors.lastname: Ibata
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001479:Basal Ganglia / Q000201:enzymology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D007031:Hypothalamus / Q000201:enzymology / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D009479:Neuropeptides / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D019001:Nitric Oxide Synthase; D010286:Paraventricular Hypothalamic Nucleus / Q000201:enzymology / Q000378:metabolism; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D051381:Rats; D017208:Rats, Wistar; D013495:Supraoptic Nucleus / Q000201:enzymology / Q000378:metabolism
chemical_list: C495403:Adcyap1 protein, rat; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases