cDNA cloning of a putative protochordate FK506-binding protein.
A tunicate (Botryllus schlossert) cDNA library was screened with a microsatellite probe. Five positive clones were sequenced, each with a 5' truncated microsatellite. One (Bs.6) revealed striking similarity to FK506 and rapamycin-binding proteins (FKBPs). Clone Bs.6 is 500 base pairs long and encodes for a putative protein of 134 amino acids. The predicted protein features the two FKBP-type peptidyl-prolyl cis-trans isomerase (PPIase) signatures and an endoplasmic reticulum retention signal. This protochordate protein is substantially similar to 12-13 kDa FKBPs, most remarkably to one of the receptors that had been proposed to mediate the immunosuppressive actions of FK506, the human FKBP-13 (62% amino acid identity and 74% similarity).
Pancer
Z
Z
Israel Oceanographic and Limnological Research, National Institute of Oceanography, Dept. of Marine Biology, Haifa, Israel.
Gershon
H
H
Rinkevich
B
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism*; D016208:Databases, Factual; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D016559:Tacrolimus / Q000378:metabolism*; D022021:Tacrolimus Binding Proteins; D014561:Urochordata / Q000378:metabolism*
D002352:Carrier Proteins; D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D022021:Tacrolimus Binding Proteins; D016559:Tacrolimus