title: The evaluation of PCNA/cyclin expression in cervical intraepithelial lesions.
abstract: This study aims at evaluating the expression of PCNA/cyclin in the cells of cervical intraepithelial lesions. The material constituted 121 colposcopic biopsies from patients with cytological and/or colposcopical evidence of condyloma (HPV) or CIN. The cases were classified as follows: 20 as koilocytosis, 31 as CIN I and HPV, 30 as CIN II and HPV, 1 as CIN III and HPV, and 7 as CIN III. Immunohistochemical detection of PCNA/cyclin was performed on paraffin sections by the avidin-extravidin method using the monoclonal antibody PC10 (DAKO). According to our results a statistically significant difference was observed between all groups of our material (P < 0.01). The expression of PC10 may thus offer useful information on the proliferation capacity of a CIN lesion with regard to the histological pattern. Moreover, this method is simple and reproducible, requiring no specialized equipment or medical staff.
authors.affiliation: Department of Clinical Cytology and Cytogenetics, Laiko Hospital, Athens, Greece.
authors.forename: P
authors.initials: P
authors.lastname: Karakitsos
authors.forename: A
authors.initials: A
authors.lastname: Kyroudes
authors.forename: C
authors.initials: C
authors.lastname: Apostolaki
authors.forename: P
authors.initials: P
authors.lastname: Paizi
authors.forename: Z
authors.initials: Z
authors.lastname: Voulgaris
authors.forename: G
authors.initials: G
authors.lastname: Alekou
authors.forename: K
authors.initials: K
authors.lastname: Kyrkou
mesh_terms: D000911:Antibodies, Monoclonal; D002584:Cervix Uteri / Q000378:metabolism / Q000473:pathology; D003218:Condylomata Acuminata / Q000378:metabolism* / Q000473:pathology; D016213:Cyclins / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D013194:Staining and Labeling; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D016213:Cyclins; D018809:Proliferating Cell Nuclear Antigen