Susceptibility of C5b-9(m) to postmortem changes.
C5b-9(m) is a specific and sensitive marker for myocardial cell necrosis. The diagnostic value of this marker would be considerably limited in forensic practice if its immuno-histochemical demonstration were hampered by putrefaction or autolysis. We could demonstrate C5b-9(m) immunohistochemically in necrotic myocardium due to infarction up to the 11th day of experimentally induced putrefaction and autolysis, when reliable demonstration of myocardial infarction with hematoxylin-eosin was no longer possible. Under the experimental conditions of this study, no false positive immunohistochemical staining occurred.
Thomsen
H
H
Institute of Forensic Medicine, Christian-Albrechts University, Kiel, Germany.
Held
H
H
D015982:Bias; D015415:Biomarkers / Q000032:analysis; D015938:Complement Membrane Attack Complex / Q000032:analysis*; D004801:Eosine Yellowish-(YS); D005069:Evaluation Studies as Topic; D005189:False Positive Reactions; D005554:Forensic Medicine / Q000379:methods; D006416:Hematoxylin; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D009203:Myocardial Infarction / Q000473:pathology*; D009336:Necrosis; D011180:Postmortem Changes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D015415:Biomarkers; D015938:Complement Membrane Attack Complex; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin