Mapping of the mAb73 epitope on Ad2 E1A proteins with random peptide libraries and deletion mutants.
mAb73, a monoclonal antibody against adenoviruses type 2 and 5 E1A, recognises an epitope within the C-terminal part of this protein. To identify the epitope we used random peptide libraries expressed on the surface of filamentous phages (Fd, M13). We found a consensus sequence homologous to the nuclear transport signal KRPRP at the C-terminus of Ad2 and Ad5 E1A. An E1A mutant deleted for these residues failed to be immunoprecipitated by mAb73, confirming that the nuclear transport signal of E1A is the epitope recognised by mAb73.
Arsenault
H
H
Department of Microbiology, Faculty of Medicine, University of Sherbrooke, Quebec, Canada.
Weber
J M
JM
D000256:Adenoviridae / Q000276:immunology; D017500:Adenovirus E1A Proteins / Q000235:genetics* / Q000276:immunology*; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology*; D016384:Consensus Sequence; D000939:Epitopes / Q000276:immunology; D015723:Gene Library; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009842:Oligopeptides / Q000235:genetics; D010455:Peptides / Q000235:genetics / Q000276:immunology; D015531:Radioimmunoprecipitation Assay; D016415:Sequence Alignment
D017500:Adenovirus E1A Proteins; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D009842:Oligopeptides; D010455:Peptides