Radioimmunoassay versus flow cytometric assay to quantify LPS-binding protein (LBP) concentrations in human plasma.
LPS-binding protein (LBP) is an acute phase protein present in plasma, that has been shown to play a major role in sensitizing monocytes to LPS. We describe here two assays to quantify LBP in plasma. The first assay made use of monophosphoryl lipid A to capture LBP in human plasma, and LBP was detected by radiolabeled anti-LBP IgG. The second assay measured LBP by flow cytometry, using LBPs ability to present LPS to the CD14 receptor present on monocytes. Both assays had a similar level of sensitivity, that allowed the quantification of LBP in human plasma.
Heumann
D
D
Department of Internal Medicine, BH-19,1011-CHUV-Lausanne, Switzerland.
Gallay
P
P
Le Roy
D
D
Landmann
R
R
Glauser
M P
MP
D000209:Acute-Phase Proteins / Q000032:analysis*; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000097:blood*; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G; D008050:Lipid A / Q000031:analogs & derivatives; D008562:Membrane Glycoproteins; D011863:Radioimmunoassay; D012680:Sensitivity and Specificity; D000099231:Lipopolysaccharide-Binding Protein
D000209:Acute-Phase Proteins; D002352:Carrier Proteins; D007074:Immunoglobulin G; D008050:Lipid A; D008562:Membrane Glycoproteins; D000099231:Lipopolysaccharide-Binding Protein; C048436:monophosphoryl lipid A