Biocytin: a neuronal tracer compatible with rapid decalcification procedures.
The compatibility of neuronal tract-tracing and decalcification procedures was examined in salamander nasal chemosensory systems. Biocytin, but not horseradish peroxidase, retained its labeling capacity following rapid decalcification of the cranial bone. The combination of biocytin tract-tracing and decalcification procedures allows the visualization of labeled neurons and/or their projections within bony regions of intact specimens.
Wirsig-Wiechmann
C R
CR
Department of Neurobiology, Bowman Gray School of Medicine, Winston-Salem, NC 27157-1010.
D000557:Ambystoma; D000818:Animals; D001370:Axonal Transport; D002628:Chemoreceptor Cells / Q000648:ultrastructure; D003648:Decalcification Technique; D006735:Horseradish Peroxidase; D008239:Lysine / Q000031:analogs & derivatives*; D009132:Muscles / Q000294:innervation; D009296:Nasal Cavity / Q000294:innervation; D009297:Nasal Mucosa / Q000294:innervation; D009833:Olfactory Pathways / Q000033:anatomy & histology*; D014562:Caudata
D006735:Horseradish Peroxidase; C013411:biocytin; D008239:Lysine