DNA strandness changes during in situ hybridization conditions.
DNA strandness changes occurring during in situ hybridization were monitored using monoclonal antibodies. Our results show that: 1) RNase induces formation of single stranded DNA, 2) after denaturation-renaturation, single stranded DNA is found principally in centromeric areas, and 3) double stranded DNA is still observed after each step.
Peretti
D
D
Istituto di Biologia, Facoltà di Medicina, Università di Cagliari, Italy.
Mezzanotte
R
R
D004247:DNA / Q000737:chemistry*; D006801:Humans; D017403:In Situ Hybridization; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D012260:Ribonucleases
D004247:DNA; D012260:Ribonucleases