[Cloning, determination of primary structure, and expression of the C-terminal segment of human cholesterol-esterase/lipase, containing the antigenic determinant of the protein, in Escherichia coli].
Clone pHICE0.9 was selected from human insulinoma cDNA library by immunoscreening with antibodies against total human insulinoma proteins. This clone contains a 0.9 kb cDNA insert and expresses a fusion protein with beta-galactosidase. Nucleotide sequences of 5'- and 3'-terminal regions of this cDNA insert show that clone pHICE0.9 expresses a protein which is identical to the C-terminal fragment (amino acids 483 to 745) of human pancreatic cholesterol esterase and Homo sapiens bile-acid-salt-stimulated lipase from milk. It is concluded that the protein fragment contains the antigenic determinant of human cholesterol esterase/lipase, and can be used for lipase determination in blood.
Chekhranova
M K
MK
Il'ina
E N
EN
Shuvalova
E R
ER
Pankov
D Iu
DIu
Pankov
Iu A
IuA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D000939:Epitopes / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics*; D006801:Humans; D007340:Insulinoma / Q000378:metabolism; D008049:Lipase / Q000235:genetics* / Q000276:immunology; D008895:Milk, Human / Q000201:enzymology; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000276:immunology; D010179:Pancreas / Q000201:enzymology; D002787:Sterol Esterase / Q000235:genetics* / Q000276:immunology
D018076:DNA, Complementary; D000939:Epitopes; D009363:Neoplasm Proteins; D002787:Sterol Esterase; D008049:Lipase