Biochemical characterization of three non-lineage antigens defined by workshop antibodies.
Monoclonal antibodies (MAbs) from three temporary clusters recognized antigens expressed on platelets and a wide variety of other tissues. To confirm the clustering of the mAbs, we biochemically characterized the antigens recognized by each cluster. TC9 (BoCD44) contained mAbs to a 95 kDa ruminant antigen, homologous to human CD44. TC11 (BoWC9) defined a 25 kDa antigen and TC7 (BoWC11) defined an antigen composed of two subunits of 49 and 79 kDa.
Naessens
J
J
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Nthale
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology; D015703:Antigens, CD / Q000737:chemistry* / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry / Q000662:veterinary; D016650:Fluorescein-5-isothiocyanate; D008970:Molecular Weight; D011233:Precipitin Tests / Q000662:veterinary
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000939:Epitopes; D016650:Fluorescein-5-isothiocyanate