title: Differences in Fluorogold and wheat germ agglutinin-horseradish peroxidase labelling of bladder afferent neurons.
abstract: Rat urinary bladder afferent neurons were significantly smaller (34%) when labelled with Fluorogold (FG) than when labelled with wheat germ agglutinin-horseradish peroxidase (WGA-HRP). This study showed that this difference was due to an artifact of tissue processing (ethanol dehydration) and was not due to uptake and transport of the two tracers by two different subpopulations of bladder afferents.
authors.affiliation: Department of Pharmacology, University of Pittsburgh, PA 15261.
authors.forename: M N
authors.initials: MN
authors.lastname: Kruse
authors.forename: S L
authors.initials: SL
authors.lastname: Erdman
authors.forename: G
authors.initials: G
authors.lastname: Puri
authors.forename: W C
authors.initials: WC
authors.lastname: de Groat
mesh_terms: D000344:Afferent Pathways / Q000166:cytology* / Q000502:physiology; D000818:Animals; D016477:Artifacts; D001370:Axonal Transport; D000431:Ethanol; D005260:Female; D005456:Fluorescent Dyes; D006652:Histological Techniques; D006735:Horseradish Peroxidase; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013266:Stilbamidines; D001743:Urinary Bladder / Q000294:innervation*; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; D000431:Ethanol; D006735:Horseradish Peroxidase