Assessment of occupational exposure to diesel exhaust. The use of an immunoassay for the determination of urinary metabolites of nitroarenes and polycyclic aromatic hydrocarbons.
In a repair shop for train engines a pilot study was conducted to investigate occupational exposure to diesel exhaust. 1-Nitropyrene was determined in stationary sampled total suspended particulate matter collected on 2 consecutive workdays. Air concentrations of particulate associated 1-nitropyrene varied from non-detectable to 5.6 ng/m3. In spot urine samples collected on Sunday, Monday and Tuesday urinary metabolites of polycyclic aromatic hydrocarbons and their nitro-substituted derivatives were determined using an immunoassay. In the urine samples of 3 diesel mechanics both cumulative and average excretion of urinary metabolites over 48 and 72 h were significantly enhanced (P < 0.05) as compared to the excreted levels in urine samples from 2 office clerks.
Scheepers
P T
PT
Toxicology Department, University of Nijmegen, The Netherlands.
Thuis
H J
HJ
Martens
M H
MH
Bos
R P
RP
D000906:Antibodies; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007118:Immunoassay; D008297:Male; D009574:Nitro Compounds / Q000378:metabolism / Q000652:urine*; D016273:Occupational Exposure; D011083:Polycyclic Compounds / Q000378:metabolism / Q000652:urine*; D011721:Pyrenes / Q000378:metabolism; D001335:Vehicle Emissions / Q000009:adverse effects*
D000906:Antibodies; D009574:Nitro Compounds; D011083:Polycyclic Compounds; D011721:Pyrenes; D001335:Vehicle Emissions; C004482:1-aminopyrene; C032668:1-nitropyrene