title: CD9 antigen is an accessory subunit of the VLA integrin complexes.
abstract: The CD9 antigen is a cell surface glycoprotein of unknown function which belongs to the tetraspans family. We demonstrate here, by precipitation, Western blotting and co-capping experiments, that this molecule is associated with a large fraction of beta 1 integrins in two cell lines, the pre-B cell line NALM-6 and the megakaryocytic cell line HEL. In HEL cells, CD9 antigen is only associated with VLA-4. In contrast, in NALM-6 cells, CD9 antigen is associated with both VLA-4 and VLA-5. On the other hand, only the beta 1 chain is co-precipitated with the CD9 antigen in transfected L cells. These data show that the CD9 antigen is associated with the beta 1 chain rather than with a particular integrin. CD9 monoclonal antibodies (mAb) did not modify the binding of HEL and NALM-6 cells to fibronectin, laminin or collagen. The association of CD9 antigen to VLA integrins is strengthened by the fact that both CD9 and anti-VLA mAb induce aggregation of the two cell lines and inhibit their migration in Transwell chambers. Because the aggregating effect, but not the inhibition of migration, is observed in CEM or CD9-transfected CEM cells, these two effects are likely to be mediated by different mechanisms.
authors.affiliation: INSERM U268, Hôpital Paul Brousse, Villejuif, France.
authors.forename: E
authors.initials: E
authors.lastname: Rubinstein
authors.forename: F
authors.initials: F
authors.lastname: Le Naour
authors.forename: M
authors.initials: M
authors.lastname: Billard
authors.forename: M
authors.initials: M
authors.lastname: Prenant
authors.forename: C
authors.initials: C
authors.lastname: Boucheix
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002449:Cell Aggregation; D016384:Consensus Sequence; D007152:Immunologic Capping; D066298:In Vitro Techniques; D019012:Integrin beta1; D016023:Integrins / Q000378:metabolism*; D007739:L Cells; D008562:Membrane Glycoproteins; D051379:Mice; D008969:Molecular Sequence Data; D017469:Receptors, Fibronectin / Q000378:metabolism*; D016029:Receptors, Very Late Antigen / Q000378:metabolism*; D060245:Tetraspanin 29; D014162:Transfection
chemical_list: D015703:Antigens, CD; C554870:Cd9 protein, mouse; D015815:Cell Adhesion Molecules; D019012:Integrin beta1; D016023:Integrins; D008562:Membrane Glycoproteins; D017469:Receptors, Fibronectin; D016029:Receptors, Very Late Antigen; D060245:Tetraspanin 29