title: The transposable element mariner can excise in non-drosophilid insects.
abstract: Plasmid-based excision assays performed in embryos of two non-drosophilid species using the mariner transposable element from Drosophila mauritiana resulted in empty excision sites identical to those observed after the excision of mariner from D. mauritiana chromosomes. In the presence of the autonomous mariner element Mos1, excision products were recovered from D. melanogaster, D. mauritiana and the blowfly Lucilia cuprina. When a hsp82 heat shock promoter-Mos1 construct was used to supply mariner transposase, excision products were also recovered from the Queensland fruitfly Bactrocera tryoni. Analysis of DNA sequences at empty excision sites led us to hypothesise that the mariner excision/repair process involves the formation of a heteroduplex at the excision breakpoint. The success of these assays suggests that they will provide a valuable tool for assessing the ability of mariner and mariner-like elements to function in non-drosophilid insects and for investigating the basic mechanisms of mariner excision and repair.
authors.affiliation: Commonwealth Scientific and Industrial Research Organisation, Division of Entomology, Canberra, Australia.
authors.forename: C J
authors.initials: CJ
authors.lastname: Coates
authors.forename: C L
authors.initials: CL
authors.lastname: Turney
authors.forename: M
authors.initials: M
authors.lastname: Frommer
authors.forename: D A
authors.initials: DA
authors.lastname: O'Brochta
authors.forename: W D
authors.initials: WD
authors.lastname: Warren
authors.forename: P W
authors.initials: PW
authors.lastname: Atkinson
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004260:DNA Repair; D004251:DNA Transposable Elements; D004175:Diptera / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic
chemical_list: D004251:DNA Transposable Elements