Fourth isoform of preprotachykinin messenger RNA encoding for substance P in the rat intestine.
Three different isoforms of preprotachykinin mRNA (PPT mRNA) encode for substance P and related neuropeptides (1). Here we report a fourth isoform of PPT mRNA which is generated by alternative exclusion of exon-7 and exon-6 from the PPT mRNA. It was present mainly in ileal smooth muscle and mucosa, colon, heart and brain and low level of this mRNA was detected in the jejunal smooth muscle and mucosa. This was not detected in the kidney or uterus. The level of this PPT mRNA was enhanced significantly by 60% during colitis in rat induced by trinitro benzene sulphonic acid.
Khan
I
I
Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
Collins
S M
SM
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D005260:Female; D007082:Ileum / Q000737:chemistry; D007413:Intestinal Mucosa / Q000737:chemistry; D007422:Intestines / Q000737:chemistry*; D008297:Male; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013373:Substance P / Q000235:genetics*; D015320:Tachykinins / Q000235:genetics*; D014018:Tissue Distribution
D011498:Protein Precursors; D012333:RNA, Messenger; D015320:Tachykinins; C044206:preprotachykinin; D013373:Substance P