Serotonin and NADPH-diaphorase in the dorsal raphe nucleus of the adult rat.
NADPH-diaphorase histochemistry, employed as a marker for nitric oxide synthase (NOS), was combined with serotonin (5-HT) immunofluorescence to investigate the relationship between NOS and 5-HT in the rat dorsal raphe nucleus. Many NADPH-diaphorase labelled cells and varicose axons were observed in the nucleus. Coexistence between NADPH-diaphorase and 5-HT occurs in cells of the dorsomedial and ventromedial subgroups but not in the lateral subgroups. Coexistence was not observed in axons, but NADPH-diaphorase labelled axons contact 5-HT/NADPH-diaphorase containing cell bodies. These findings have implications for the role of nitric oxide in 5-HT pathways and for the mechanism of action of 5-HT neurotoxins.
Wotherspoon
G
G
Department of Physiology, United Medical School of Guy's Hospital, London, UK.
Albert
M
M
Rattray
M
M
Priestley
J V
JV
D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D000818:Animals; D001369:Axons / Q000276:immunology; D015415:Biomarkers; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009411:Nerve Endings / Q000201:enzymology; D019001:Nitric Oxide Synthase; D011903:Raphe Nuclei / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism*
D015415:Biomarkers; D012701:Serotonin; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase