title: Poliovirus neutralization by antibodies to internal epitopes of VP4 and VP1 results from reversible exposure of these sequences at physiological temperature.
abstract: Antisera were raised against peptide sequences that are normally internal in the poliovirus virion. These antisera contain neutralizing activity, but this neutralizing activity is dependent on coincubation of the virus and antisera at 37 degrees C. Immunoprecipitation analyses demonstrate that the neutralization is due to exposure of these normally internal sequences at 37 degrees C and subsequent antibody binding. Exposure of these sequences is reversible. These data demonstrate that the poliovirus particle is a dynamic entity that is capable of undergoing conformational alterations at physiological temperatures. This conformational flexibility provides an explanation for earlier observations of virus neutralization by antibodies to internal epitopes which can be accommodated within the framework of existing models for antibody-mediated neutralization of viral infectivity. Analogies between the sequences which are reversibly exposed at 37 degrees C with those which are irreversibly exposed upon receptor binding suggest that the observed conformational dynamics also may play a role in cell entry.
authors.affiliation: Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.forename: A G
authors.initials: AG
authors.lastname: Yafal
authors.forename: Y M
authors.initials: YM
authors.lastname: Lee
authors.forename: J
authors.initials: J
authors.lastname: Hogle
authors.forename: M
authors.initials: M
authors.lastname: Chow
mesh_terms: D000914:Antibodies, Viral; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000737:chemistry; D002213:Capsid / Q000737:chemistry / Q000276:immunology*; D036022:Capsid Proteins; D000939:Epitopes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D009500:Neutralization Tests; D017955:Poliovirus / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D011487:Protein Conformation; D013696:Temperature
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; C442540:VP1 protein, Poliovirus; C060339:VP4 protein, Rotavirus