Species differences in lectin binding to pulmonary cells: Soybean agglutinin (SBA) as a marker of type I alveolar epithelial cells and alveolar macrophages in mini pigs.
We compared lectin staining patterns in rat and mini pig tissues of normal and fibrotic (irradiation-induced) lungs. Two lectins were studied: Dolichos biflorus (DBA) and Soybean (SBA). Both lectins strongly stained a subpopulation of alveolar macrophages. In the rat, DBA positive macrophages were a subpopulation of the SBA binding cells. In mini pig lungs, a further specific binding of DBA and SBA was observed: DBA reacted with endothelia, and SBA stained the alveolar type I cells. Double immunofluorescence experiments using a type II cell-specific cytokeratin antibody confirmed the selective reactivity of SBA with type I cells, which was also present in fibrotic areas with epithelial cell proliferation.
Kasper
M
M
Institute of Pathology, Medical Academy Carl Gustav Carus, Dresden, Germany.
Haroske
G
G
Müller
M
M
D000818:Animals; D001665:Binding Sites; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D007633:Keratins / Q000032:analysis / Q000276:immunology; D037102:Lectins / Q000378:metabolism*; D008168:Lung / Q000473:pathology*; D016676:Macrophages, Alveolar / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D037121:Plant Lectins; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D011658:Pulmonary Fibrosis / Q000473:pathology*; D051381:Rats; D011916:Rats, Inbred F344; D030262:Soybean Proteins; D013025:Glycine max; D013552:Swine; D013556:Swine, Miniature; D010835:Phytohemagglutinins
D007633:Keratins; D037102:Lectins; D037121:Plant Lectins; D030262:Soybean Proteins; C036554:dolichos biflorus agglutinin; C028824:soybean lectin; D010835:Phytohemagglutinins