CD40 expression in Hodgkin's disease.
CD40 is a transmembrane protein that belongs to a superfamily of proteins related to nerve growth factor receptor. CD40 is expressed on B cells and some B cell malignancies. It appears to be involved in B cell proliferation and the prevention of apoptosis in germinal center cells, which is accompanied by expression of bcl-2. Its expression is up-regulated by the EBV protein latent membrane protein-1 and cytokines interleukin-4 and interferon-gamma. The expression of CD40 in 37 cases of Hodgkin's disease and 23 cases of non-Hodgkin's lymphoma (11 T cell lymphomas and 12 B cell lymphomas) was examined by paraffin section immunohistochemistry using the BB-20 monoclonal antibody. In 26 of 37 cases of Hodgkin's disease the Reed-Sternberg cells showed strong membrane or cytoplasmic expression of CD40. Only 3 of 23 non-Hodgkin's lymphomas showed any expression of CD40 and then only weakly. There was no correlation between expression of bcl-2 or latent membrane protein-1 with CD40 expression. These results show that there is probable hyperexpression of CD40 in Hodgkin's disease and suggest that dysregulation of CD40 expression may play a role in the pathogenesis of Hodgkin's disease.
O'Grady
J T
JT
Department of Pathology, University of Edinburgh, United Kingdom.
Stewart
S
S
Lowrey
J
J
Howie
S E
SE
Krajewski
A S
AS
D015703:Antigens, CD / Q000032:analysis*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis; D019013:CD40 Antigens; D006689:Hodgkin Disease / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008221:Lymphoid Tissue / Q000276:immunology*; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology; D016539:Reed-Sternberg Cells / Q000276:immunology / Q000378:metabolism; D012016:Reference Values; D013194:Staining and Labeling; D014763:Viral Matrix Proteins / Q000032:analysis
D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D000956:Antigens, Viral; D019013:CD40 Antigens; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins