Design of degenerate oligonucleotide primers for cloning of G-protein alpha subunits.
Wilkie
T M
TM
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235.
Aragay
A M
AM
Watson
A J
AJ
Simon
M I
MI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000138:chemical synthesis*; D018076:DNA, Complementary / Q000096:biosynthesis; D019204:GTP-Binding Proteins / Q000096:biosynthesis*; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis; D016133:Polymerase Chain Reaction / Q000379:methods*; D012194:RNA-Directed DNA Polymerase; D011994:Recombinant Proteins / Q000096:biosynthesis*; D015183:Restriction Mapping
D017931:DNA Primers; D018076:DNA, Complementary; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D016376:Oligonucleotides, Antisense; D011994:Recombinant Proteins; D012194:RNA-Directed DNA Polymerase; D019204:GTP-Binding Proteins