title: Effects of aprotinin in platelet aggregation and cytosolic free calcium in swine platelets.
abstract: Aprotinin inhibited platelet aggregation induced by thrombin (0.25 U.ml-1) with IC50 200 kIU.ml-1, and inhibited the rise of cytosolic free calcium concentration in platelets stimulated by thrombin (0.1 U.ml-1) in the absence and in the presence of Ca2+ 0.5 mmol.L-1 (IC50 117 and 50 kIU.ml-1, respectively), but had no effect on the amounts of actin and myosin heavy chain associated with cytoskeletons. These suggest that aprotinin is an anti-platelet agent and may exert its action through inhibiting the Ca2+ flux.
authors.affiliation: Department of Medical Biochemistry, Guangdong Medical College, Zhanjiang, China.
authors.forename: C
authors.initials: C
authors.lastname: Huang
authors.forename: Y M
authors.initials: YM
authors.lastname: Qin
authors.forename: N C
authors.initials: NC
authors.lastname: Liang
mesh_terms: D000818:Animals; D007611:Aprotinin / Q000494:pharmacology*; D002118:Calcium / Q000097:blood*; D003598:Cytoskeletal Proteins / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D013552:Swine
chemical_list: D003598:Cytoskeletal Proteins; D010975:Platelet Aggregation Inhibitors; D007611:Aprotinin; D002118:Calcium