The effect of nonionic detergents on the activity and/or stability of rat brain nitric oxide synthase.
The results of this study indicate that the addition of low concentrations of a nonionic detergent such as those represented by the Tween, Brij, or Triton classes causes an apparent activation of nitric oxide synthase. It is possible that this apparent activation is due to the ability of these detergents to stabilize the protein. The stabilizing influence of the detergents may be a result of inhibiting the dissociation of the dimeric protein into monomers or the dissociation of an essential cofactor or prosthetic group from the active enzyme. Regardless of the mechanism of action, the addition of low concentrations of nonionic detergents results in longer and increased nitric oxide synthase activity and may be an important tool for those involved in enzymological studies of nitric oxide synthase.
Komori
Y
Y
Department of Pharmacology, UCLA School of Medicine 90024-1735.
Chiang
K T
KT
Fukuto
J M
JM
D000594:Amino Acid Oxidoreductases / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002531:Cerebellum / Q000201:enzymology*; D003902:Detergents / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability / Q000187:drug effects; D019001:Nitric Oxide Synthase; D011092:Polyethylene Glycols / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D051381:Rats
D003902:Detergents; D011136:Polysorbates; D011092:Polyethylene Glycols; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases