[The cause-effect relationships between different indices of radiation lesions of the thymocytes].
The dependence of the kinetics of different thymocyte injuries of the experimental conditions was investigated. It was shown that the rate of cell death detecting by cell staining decreases with the increase of the dye molecular size. Different organic substances decrease the staining rate of the dead cells with trypan blue but increase the rate of DNA fragmentation. They change the kinetics of cell injuries development only after but not during irradiation. Development of pycnoses forestalls the DNA fragmentation. Basing on these findings the conclusion was made that the time-course of different thymocyte injuries development cannot be used for a statement what type of injuries--of plasmic membrane or cell nucleus--is initial.
Eĭdus
L Kh
LKh
Shaposhnikova
V V
VV
Dobrovinskaia
O P
OP
Korystov
Iu N
IuN
D000818:Animals; D016923:Cell Death / Q000528:radiation effects; D002478:Cells, Cultured / Q000166:cytology / Q000528:radiation effects; D003470:Culture Media; D004247:DNA / Q000528:radiation effects; D007399:Interphase / Q000528:radiation effects; D008297:Male; D011833:Radiation Injuries, Experimental / Q000209:etiology* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling; D013950:Thymus Gland / Q000166:cytology / Q000528:radiation effects*
D003470:Culture Media; D004247:DNA