Cyclic regulation of B220 antigen expression in immature B cell lines.
Three independent immature B cell lines transformed with temperature-sensitive mutants of Abelson murine leukaemia virus (A-MuLV) persistently expressed B220 antigen detected by both of the anti-B220 antibodies RA3-6B2 and 14.8 during culture at the permissive temperature (35 degrees C). RA3-6B2 recognizes the B220 eptiope and that 14.8 is a CD45RA antibody. However, when the culture temperature was shifted up to the non-permissive temperature (39 degrees C) and the culture was continued for 2-3 weeks, a population of the cells (RA3-6B2-14.8+ cells) that lost RA3-6B2 antigen appeared in all three cell lines. From these populations, RA3-6B2-14.8+ subclones were independently isolated by limiting dilution and the phenotype was stable during culture at the permissive temperature. When the culture temperature of the RA3-6B2-14.8+ subclones was shifted up to the non-permissive temperature and the culture was continued for 2-3 weeks, RA3-6B2 antigen was re-expressed in a subpopulation of the RA3-6B2-14.8+ cells. These results demonstrated cyclic regulation of B220 antigen expression during proliferation and/or differentiation of immature B cell lines.
Oka
Y
Y
Department of Medicine III, Osaka University Medical School, Japan.
Shimizu
Y
Y
Tsukada
S
S
Sugiyama
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology*; D002453:Cell Cycle; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D005434:Flow Cytometry; D009052:Leukemia Virus, Murine; D017493:Leukocyte Common Antigens; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D017493:Leukocyte Common Antigens