High endothelial venules and cell adhesion molecules in B-cell chronic lymphocytic leukaemia and related low grade B-cell lymphoma/leukaemia: II. Expression of cell adhesion molecules in lymph nodes biopsies.
The expression of cell adhesion molecules (LECAM-1, LFA-1, VLA-4, ICAM-1 and CD44) of lymph nodes from B-cell chronic lymphocytic leukaemia (B-CLL; mature: 16 cases, immature: 8), lymphocytic lymphoma (LL; M:5, IM:2) and reactive lymph nodes (10) were investigated in frozen tissue sections. In order to assess the B- and T-cell compartments of the lymph nodes some additional markers CD3, CD20, CD45 RO were used, and for follicular dendritic reticulum cells (FDCs) CD35. The expression of the LECAM-1 molecule was correlated to the expression of activation (CD23, CD25) and proliferation (Ki67) markers. Findings, in accordance with the relevant data of the literature, indicate that B-CLL and LL show and identical adhesion profile to the mantle zone of the germinal centres of reactive lymph nodes; namely, they were CD44+/LECAM-1+/VLA-4+. The T-zones of the reactive lymph nodes were characterized by an LFA-1+ and the follicles by an ICAM-1+ pattern, while in B-CLL and LL cases the LFA-1+ and ICAM-1+ were detected without coexpression of these molecules in only a small number of cases. The ratio and location of CD3+ and LFA-1+ cells was very similar in lymph nodes from B-CLL and LL, and from this fact may arise the suspicion that LFA-1+ reported in LL cases derive from the sometimes significant T-cell compartment of the diseased lymph nodes. The LECAM-1 molecule did not show any correlation with the investigated activation/proliferation markers.
Csanaky
G
G
Department of Pathology, University Medical School of Pécs, Hungary.
D001706:Biopsy; D002352:Carrier Proteins / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis*; D006801:Humans; D018960:Hyaluronan Receptors; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D019041:L-Selectin; D015448:Leukemia, B-Cell / Q000473:pathology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology; D008198:Lymph Nodes / Q000098:blood supply*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D016393:Lymphoma, B-Cell / Q000473:pathology*; D011956:Receptors, Cell Surface / Q000032:analysis; D016201:Receptors, Lymphocyte Homing / Q000032:analysis; D016029:Receptors, Very Late Antigen / Q000032:analysis
D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D016169:Lymphocyte Function-Associated Antigen-1; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D016029:Receptors, Very Late Antigen; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin