Small-scale magnetic isolation of mRNA and synthesis of cDNA in 96-well plates.
Multiple small biological samples can be simultaneously prepared for RT-PCR by magnetically isolating mRNA and synthesizing cDNA in 96-well plates. The resulting accuracy, high throughput and ease of use facilitate commercial and clinical applications.
Oaklander
A L
AL
Department of Neurology, Johns Hopkins Medical Institutions, Baltimore, Maryland.
Ekenberg
S J
SJ
D018076:DNA, Complementary / Q000138:chemical synthesis*; D008280:Magnetics; D011061:Poly A; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase
D018076:DNA, Complementary; D012333:RNA, Messenger; D011061:Poly A; D012194:RNA-Directed DNA Polymerase