T cell epitope analysis with peptides simultaneously synthesized on cellulose membranes: fine mapping of two DQ dependent epitopes.
Several MHC class II restricted, CD4+ human T cell clones from three donors were induced with soluble matrix protein of influenza virus. The epitopes recognized by these clones were mapped using a complete set of overlapping 15-mer peptides synthesized with the newly developed SPOT method which allows the simple simultaneous synthesis of numerous peptides on cellulose membranes. Fine analysis of two clones by truncation of the stimulatory peptide by single subsequent amino acids from either the NH2- or the COOH-terminus revealed the minimal stimulatory determinants of two DQ dependent T cell epitopes.
Adler
S
S
GBF, Braunschweig, Germany.
Frank
R
R
Lanzavecchia
A
A
Weiss
S
S
D000595:Amino Acid Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002482:Cellulose; D018604:Epitope Mapping / Q000379:methods*; D000939:Epitopes / Q000276:immunology*; D006683:HLA-DQ Antigens / Q000276:immunology; D006801:Humans; D009980:Influenza A virus; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*; D014763:Viral Matrix Proteins
D000939:Epitopes; D006683:HLA-DQ Antigens; C031540:M-protein, influenza virus; D010455:Peptides; D014763:Viral Matrix Proteins; D002482:Cellulose