Nucleotide sequence statistical analysis of pauses in RNA elongation by Escherichia coli RNA polymerase.
A convenient motif-searching program has been developed, based on a double correlation algorithm, for analysis of the pulse character of Escherichia coli transcription. Activity in the zone of minimal pause formation (-1,2 bp) is precisely determined. Oligonucleotides (di-, tri- and tetranucleotides) are randomized by their pause-generating activity. 'CATG' and 'CATGC' are detected which coincide with the primary structure of RNA associated with distinctive delays in biologically meaningful situations.
Fedoseyeva
V B
VB
Institute of Molecular Genetics, Russian Academy of Science, Moscow.
Otenko
S A
SA
Alexndrov
A A
AA
D000465:Algorithms; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005069:Evaluation Studies as Topic; D012329:RNA, Bacterial / Q000235:genetics* / Q000378:metabolism; D017423:Sequence Analysis, RNA / Q000379:methods* / Q000706:statistics & numerical data; D012984:Software; D014158:Transcription, Genetic
D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases