title: Secretion of prostaglandins and endothelin-1 by decidual endothelial cells from normal and preeclamptic pregnancies: comparison with human umbilical vein endothelial cells.
abstract: OBJECTIVE
  An increasing amount of circumstantial evidence points to the maternal endothelial cell as centrally involved in the syndrome of preeclampsia. The purposes of this study were (1) to compare the secretion of vasoactive substances by maternal decidual endothelial cells with that of umbilical vein endothelial cells, widely used as a surrogate for endothelial cells in general, and (2) to compare secretion of the same vasoactive substances by decidual endothelial cells from normal and preeclamptic pregnancies.
  
  
  METHODS
  Endothelial cells were isolated from umbilical veins and from decidual biopsy specimens collected at cesarean section delivery from both normal and preeclamptic women. Cells were maintained in culture until passage 2, when secretion by the three endothelial cell populations of the vasodilators prostaglandin E2 and prostacyclin and the vasoconstrictor endothelin-1 was examined. In addition to control incubations, their responses to stimulation and suppression of secretion were compared.
  
  
  RESULTS
  In control incubations normal decidual endothelial cells secreted lower amounts of prostacyclin, prostaglandin E2, and endothelin than did human umbilical vein endothelial cells. All cell types had qualitatively similar responses to the stimuli used, but quantitatively different responses were noted between human umbilical vein endothelial cells and normal decidual endothelial cells for all metabolites examined. Preeclamptic decidual endothelial cells secreted significantly more prostaglandin E2 than did normal decidual endothelial cells in response to stimulation.
  
  
  CONCLUSIONS
  We have delineated levels of secretion of vasoactive substances by human late pregnancy decidual endothelial cells and their responses to manipulation of secretory pathways. There are differences between this endothelial cell population and human umbilical vein endothelial cells (which are widely used as a surrogate for maternal endothelial cells). The differences between normal and preeclamptic decidual endothelial cells in prostaglandin E2 secretion may point to altered regulation of arachidonic acid metabolic pathways in preeclampsia.
authors.affiliation: Department of Renal Medicine, Royal North Shore Hospital, St. Leonard's, New South Wales, Australia.
authors.forename: E D
authors.initials: ED
authors.lastname: Gallery
authors.forename: J
authors.initials: J
authors.lastname: Rowe
authors.forename: S
authors.initials: S
authors.lastname: Campbell
authors.forename: T
authors.initials: T
authors.lastname: Hawkins
mesh_terms: D002478:Cells, Cultured; D002585:Cesarean Section; D003656:Decidua / Q000098:blood supply*; D016232:Endothelins / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D011464:Epoprostenol / Q000378:metabolism*; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D012016:Reference Values; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014471:Umbilical Veins
chemical_list: D016232:Endothelins; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D011464:Epoprostenol; D006854:Hydrocortisone; D007213:Indomethacin