title: Tyrosine kinase JAK1 is associated with the granulocyte-colony-stimulating factor receptor and both become tyrosine-phosphorylated after receptor activation.
abstract: Granulocyte-colony-stimulating factor (G-CSF) stimulates the proliferation and differentiation of cells of the neutrophil lineage by interaction with a specific receptor. Early signal transduction events following G-CSF receptor activation were studied. We detected tyrosine phosphorylation of both the G-CSF receptor and the protein tyrosine kinase JAK1 following G-CSF binding to the human G-CSF receptor. In vitro, the kinase activity of JAK1 was increased by G-CSF stimulation. Coimmunoprecipitation of JAK1 with the G-CSF receptor suggested a physical association which existed prior to G-CSF stimulation.
authors.affiliation: Melbourne Tumour Biology Branch, Ludwig Institute for Cancer Research, Victoria, Australia.
authors.forename: S E
authors.initials: SE
authors.lastname: Nicholson
authors.forename: A C
authors.initials: AC
authors.lastname: Oates
authors.forename: A G
authors.initials: AG
authors.lastname: Harpur
authors.forename: A
authors.initials: A
authors.lastname: Ziemiecki
authors.forename: A F
authors.initials: AF
authors.lastname: Wilks
authors.forename: J E
authors.initials: JE
authors.lastname: Layton
mesh_terms: D000818:Animals; D016466:CHO Cells; D002455:Cell Division; D006224:Cricetinae; D006801:Humans; D053613:Janus Kinase 1; D010766:Phosphorylation; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D016188:Receptors, Granulocyte Colony-Stimulating Factor; D019000:Phosphotyrosine; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases; C507904:JAK1 protein, human; D053613:Janus Kinase 1