Preservation and contrast without osmication or section staining.
Conventional treatment of tissues for sectioning and transmission electron microscopy uses aldehyde fixation and osmium tetroxide postfixation. Although the result is aesthetically pleasing, osmication destroys some cell components and reduces the chemical activity of others, such as reaction with antibodies and lectins. We have found that aldehyde fixation followed by uranyl acetate preserves and contrasts most structures and visualizes some that are not easily seen after osmication. Aldehyde/UA treated tissues have enough contrast to be observed without section staining while retaining some of the chemical activity that is lost through osmication. Sections of tissues with good preservation and contrast can be used for immunogold and lectin-gold labelling of at least some molecules.
Locke
M
M
Department of Zoology, University of Western Ontario, London, Canada.
D000447:Aldehydes; D000818:Animals; D005404:Fixatives; D007150:Immunohistochemistry; D037102:Lectins; D007915:Lepidoptera; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods*; D009942:Organometallic Compounds; D009993:Osmium Tetroxide; D013194:Staining and Labeling; D016707:Tissue Fixation / Q000379:methods; D014021:Tissue Preservation / Q000379:methods*
D000447:Aldehydes; D005404:Fixatives; D037102:Lectins; D009942:Organometallic Compounds; C005460:uranyl acetate; D009993:Osmium Tetroxide