Simultaneous demonstration of infecting fungi and DNA-synthesizing epithelial cells in the skin.
An excellent staining method to demonstrate in vivo dermatophytes and DNA-synthesizing cells in the skin is described. Deparaffinized sections of guinea pig skin, infected with dermatophytes and given bromodeoxyuridine (BrdU) by intraperitoneal injection, were used. Periodic acid-Schiff (PAS) stain in combination with an immunohistochemical stain using anti-BrdU monoclonal antibody allowed simultaneous visualization of fungi and DNA-synthesizing epithelial cells in the skin. Many epithelial cells near the infecting fungi in follicles and interfollicular skin were labelled with BrdU. Only a few BrdU-positive cells were observed in the skin of uninfected animals.
Fujita
S
S
Department of Dermatology, Niigata University School of Medicine, Japan.
Tezuka
M
M
Kaise
A
A
Ito
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine / Q000276:immunology; D004247:DNA / Q000096:biosynthesis; D003881:Dermatomycoses / Q000175:diagnosis*; D004847:Epithelial Cells; D004848:Epithelium / Q000254:growth & development; D006168:Guinea Pigs; D008297:Male; D010503:Periodic Acid-Schiff Reaction / Q000379:methods*; D012867:Skin / Q000382:microbiology* / Q000473:pathology; D013194:Staining and Labeling; D014249:Trichophyton / Q000276:immunology / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D004247:DNA; D001973:Bromodeoxyuridine