title: Basic fibroblast growth factor experimentally induced choroidal angiogenesis in the minipig.
abstract: Basic fibroblast growth factor (bFGF), a soluble mitogen, has been isolated and purified from various organs, including the retina. In vivo angiogenic activity of bFGF has been demonstrated with several assays. An experimental model of choroidal neovascularization was developed in the mini pig by perfusion of recombinant human bFGF through an osmotic minipump. Endogenous bFGF and bFGF receptors were localized in the normal pig retina by immunohistochemistry and autoradiography after binding. The perfusion of exogenous bFGF induced well-organized new vessels along the last 3 mm of the catheter in the suprachoroidal space. This neovascularization did not penetrate the normal Bruch's membrane. Vascular cells (identified by von Willebrand factor antibody staining) increased in number and in surface from the proximal part to the end of the intraocular catheter in all bFGF perfused eyes. In eyes perfused with phosphate buffered saline (controls), but not in the bFGF perfused eyes, an inflammatory response occurred (identified by a macrophage specific antibody). These results demonstrate that choroidal angiogenesis can be achieved without an inflammatory response by perfusing an excess of bFGF in the suprachoroidal space.
authors.affiliation: Unite de Recherches INSERM U.118, Paris, France.
authors.forename: G
authors.initials: G
authors.lastname: Soubrane
authors.forename: S Y
authors.initials: SY
authors.lastname: Cohen
authors.forename: T
authors.initials: T
authors.lastname: Delayre
authors.forename: J
authors.initials: J
authors.lastname: Tassin
authors.forename: M P
authors.initials: MP
authors.lastname: Hartmann
authors.forename: G J
authors.initials: GJ
authors.lastname: Coscas
authors.forename: Y
authors.initials: Y
authors.lastname: Courtois
authors.forename: J C
authors.initials: JC
authors.lastname: Jeanny
mesh_terms: D000818:Animals; D002829:Choroid / Q000098:blood supply* / Q000473:pathology; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000378:metabolism; D005169:Factor VIII / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000633:toxicity*; D015918:Infusion Pumps, Implantable; D009389:Neovascularization, Pathologic / Q000209:etiology* / Q000473:pathology; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism; D012160:Retina / Q000378:metabolism; D013552:Swine; D013556:Swine, Miniature
chemical_list: D017468:Receptors, Fibroblast Growth Factor; D016222:Fibroblast Growth Factor 2; D005169:Factor VIII