title: Fc gamma RI and Fc gamma RIII on polymorphonuclear leucocytes in cord blood.
abstract: Fc gamma RI and Fc gamma RIII expression on polymorphonuclear leucocytes in cord blood from seven normal infants was investigated by flow cytometry. Fc gamma R expression on fresh polymorphonuclear leucocytes in whole blood samples and in blood samples incubated with or without interferon gamma (IFN-gamma) for 48 hours was also studied. The percentage of Fc gamma RIII positive polymorphonuclear leucocytes in cord blood (73.3%) was significantly lower than that in adult controls (95.9%). The mean fluorescence intensity of Fc gamma RIII was significantly increased on cord polymorphonuclear leucocytes by the incubation with IFN-gamma. Fresh cord polymorphonuclear leucocytes expressed only a small amount of Fc gamma RI as adult polymorphonuclear leucocytes. The percentage of Fc gamma RI positive polymorphonuclear leucocytes induced with IFN-gamma was significantly higher in cord blood (62.3%) than in adult controls (30.3%). It is possible that decreased expression of Fc gamma RIII is a factor in the susceptibility of newborns to infection. High expression of Fc gamma RI stimulated with IFN-gamma in neonates could have a compensatory role against decreased immunological function.
authors.affiliation: Laboratory and Institute, Tokyo Women's Medical College, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Nishida
authors.forename: T
authors.initials: T
authors.lastname: Kuratsuji
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D007231:Infant, Newborn; D019012:Integrin beta1; D007371:Interferon-gamma / Q000276:immunology; D018950:Lipopolysaccharide Receptors; D009504:Neutrophils / Q000737:chemistry*; D017452:Receptors, IgG / Q000032:analysis*; D013997:Time Factors
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D019012:Integrin beta1; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG; D007371:Interferon-gamma