Delivery of growth factor to wounds using a genetically engineered biological bandage.
Increasing the rate of wound healing of acute wounds and promoting the closure of chronic ulcers is an important goal in wound therapy. Growth factors have been shown to facilitate this process; however, the systems described for growth factor delivery are not ideal. In the present report we demonstrate the feasibility of a new method of delivering growth factors to the wound site using a genetically engineered biological bandage. The bandage consists of keratinocytes (SCC-13 cells) that are engineered by gene transfer to produce high levels of bovine growth hormone (bGH). bGH was selected for these studies because it can be easily distinguished from rat and human growth hormone in wound fluids and culture medium. The bGH-producing cells are contained and maintained in serum-free medium inside an envelope composed of a low protein binding, 0.2 micron pore size, polysulfone membrane. The genetically engineered cells cannot escape from the bandage, but the bGH is freely released into the surrounding culture medium. When placed onto a full-thickness, surgically generated wound on rats, the cells within the bandage continue to produce and release bGH into the wound for at least 3 days. This system is a safe and reliable way of providing real-time delivery of any desired biomolecule into the wound site.
Andreatta-van Leyen
S
S
Department of Physiology/Biophysics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4970.
Smith
D J
DJ
Bulgrin
J P
JP
Schafer
I A
IA
Eckert
R L
RL
D000818:Animals; D001684:Biological Dressings; D002417:Cattle; D002460:Cell Line; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D013006:Growth Hormone / Q000096:biosynthesis / Q000235:genetics / Q000627:therapeutic use*; D006801:Humans; D015603:Keratinocytes; D051379:Mice; D008819:Mice, Nude; D010957:Plasmids; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured; D014947:Wounds and Injuries / Q000378:metabolism / Q000628:therapy*
D012313:RNA; D013006:Growth Hormone