title: Measurement by 32P-postlabeling of (+/-)anti-benzo[a]pyrene-diolepoxide-N2-deoxyguanosine adduct persistence in unstimulated human peripheral blood lymphocytes.
abstract: In order to study the relative importance of endogenous and environmental factors for the individual relation between DNA damage and DNA excision repair, a method was developed for measuring quantitatively the persistence of N2-deoxyguanosine adducts formed in non-stimulated isolated human peripheral blood lymphocytes after in vitro incubation with 0.2 microM (+/-)anti-BPDE, applying 32P-postlabeling. Total binding of radiolabeled (+/-)anti-BPDE to DNA and its removal has been studied previously in human peripheral blood lymphocytes, but the method presented here enables the direct investigation of repair of the main (+/-)anti-BPDE-DNA adduct, which is implicated in benzo[a]pyrene-induced mutagenesis. Using this method, it was found that in lymphocytes, obtained from 5 individuals, most (+/-)anti-BPDE-N2-dG adducts are removed within the first 24 h after treatment, while interindividual differences appear to exist in both adduct formation and rate and extent of removal.
authors.affiliation: Department of Health Risk Analysis and Toxicology, University of Limburg, Maastricht, Netherlands.
authors.forename: R H
authors.initials: RH
authors.lastname: Stierum
authors.forename: M H
authors.initials: MH
authors.lastname: van Herwijnen
authors.forename: L M
authors.initials: LM
authors.lastname: Maas
authors.forename: G J
authors.initials: GJ
authors.lastname: Hageman
authors.forename: J C
authors.initials: JC
authors.lastname: Kleinjans
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000031:analogs & derivatives* / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D018736:DNA Adducts; D004249:DNA Damage; D004252:DNA Mutational Analysis / Q000379:methods; D004260:DNA Repair / Q000502:physiology*; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D010761:Phosphorus Radioisotopes
chemical_list: D018736:DNA Adducts; D010761:Phosphorus Radioisotopes; C043931:benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; C040060:7,8-dihydroxy-9,10-epoxide-7,8,9,10-tetrahydrobenzo(a)pyrene-10-deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine