[CD14-positive and nonspecific esterase-positive neutrophils in a patient with refractory anemia with excess of blasts in transformation].
A 34-year-old man was admitted with lumbago and anemia in November 1992. Hematological examination revealed an Hb 9.2g/dl, WBC count 13,500 microliters (33% blasts), and monocyte count 3,400/microliters. Bone marrow examination showed hyperplasia with dysplasia in trilineage blood cells and increased blasts (21.8%). A diagnosis of refractory anemia with excess of blasts in transformation (RAEB in T) was made. Cytochemical examination revealed the neutrophils in the peripheral blood were 66.5% positive for alpha-naphthyl butyrate esterase inhibited by sodium fluoride, 4.0% positive for peroxidase and 75% positive for alkaline phosphatase. The results of immuno-alkaline phosphatase stainings (avidin biotin alkaline phosphatase complex method) of neutrophils were as follows; CD16 (94.5%), CD24 (91.0%), CD13 (93.0%), CD14 (52.5%), CD33 (39.0%), CD36 (16.5%), HLA-DR (17.0%). These neutrophils exhibited monocyte-specific features and failed to show characteristics of neutrophils.
Kobayashi
S
S
Division of Clinical Laboratory, Kanagawa Cancer Center.
Yamamoto
T
T
Akiba
C
C
Katayama
N
N
Sakai
K
K
Yamaguchi
M
M
Maruta
A
A
Ogawa
K
K
Fujisawa
S
S
Sakai
R
R
D000328:Adult; D000754:Anemia, Refractory, with Excess of Blasts / Q000201:enzymology / Q000276:immunology*; D015703:Antigens, CD / Q000097:blood*; D015214:Antigens, Differentiation, Myelomonocytic / Q000097:blood*; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D006801:Humans; D018950:Lipopolysaccharide Receptors; D008297:Male; D009504:Neutrophils / Q000201:enzymology / Q000276:immunology*
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D018950:Lipopolysaccharide Receptors; D002265:Carboxylic Ester Hydrolases; C021855:naphthylbutyrate esterase