Transglycosylation reaction of maltotriose-forming amylase from Streptomyces griseus.
A maltotriose-forming amylase from Streptomyces griseus produced predominantly p-nitrophenyl alpha-maltotetraoside through a transglycosylation reaction from maltotetraose as a donor and p-nitrophenyl alpha-D-glucopyranoside as an acceptor in an organic co-solvent. With p-nitrophenyl beta-D-glucopyranoside acceptor, the enzyme catalyzed the formation of an alpha-(1-->3)-linked tetrasaccharide (p-nitrophenyl 3(1)-O-alpha-maltotriosyl-beta-D-glucopyranoside) in a yield of 16%, based on the acceptor added with its isomer p-nitrophenyl beta-maltotetraoside. This was also the case for the formation of o-chloro-p-nitrophenyl 3(1)-O-alpha-maltotriosyl-beta-D-glucopyranoside with o-chloro-p-nitrophenyl beta-D-glucopyranoside acceptor. The results shows that the anomeric configuration of the aryl group in the glucosyl acceptors had an effect on the position of transglycosylation.
Usui
T
T
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Japan.
Murata
T
T
Yabuuchi
Y
Y
Ogawa
K
K
D000681:Amylases / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002384:Catalysis; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis*; D012997:Solvents; D013305:Streptomyces griseus / Q000201:enzymology*; D013696:Temperature
D009844:Oligosaccharides; D012997:Solvents; C037063:4-nitrophenyl-alpha-maltotetraose; D000681:Amylases; C086530:maltotriose-forming amylase