Heterogeneity in calbindin-D28k expression in oxytocin-containing magnocellular neurons of the rat hypothalamus.
We have used a double-labeling immunofluorescence method to examine whether oxytocin-containing magnocellular neurons possess a calcium-binding protein, calbindin-D28k, in the hypothalamus of the rat. In the supraoptic nucleus, most oxytocin-immunoreactive cells were also stained for calbindin-D28k. However, in the magnocellular part of the paraventricular nucleus nearly all oxytocin-labeled cells were devoid of calbindin-D28k. In the anterior commissural nucleus, approximately one-third of oxytocin-stained cells were also calbindin-D28k-immunoreactive, but the other cells were negative for calbindin-D28k. This study indicates that there may be distinct chemical features between oxytocin-containing magnocellular neurons of the supraoptic nucleus compared to those of the paraventricular nucleus.
Arai
R
R
Department of Anatomy, Fujita Health University, School of Medicine, Aichi, Japan.
Jacobowitz
D M
DM
Deura
S
S
D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D005455:Fluorescent Antibody Technique; D007031:Hypothalamus / Q000737:chemistry* / Q000166:cytology; D008297:Male; D009474:Neurons / Q000737:chemistry*; D010121:Oxytocin / Q000032:analysis* / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods; D013495:Supraoptic Nucleus / Q000737:chemistry
C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D064030:S100 Calcium Binding Protein G; D010121:Oxytocin