Effect of minoxidil on the mobility and differentiation of cultivated human keratinocytes.
The mode of topical action of minoxidil (U-10,858, CAS 38304-91-5) ist not entirely clear. The influence of minoxidil on the ultimate differentiation of keratinocytes was investigated. Human keratinocytes (HaCaT-cells) were incubated with minoxidil containing culture medium (10-250 micrograms/ml). Minoxidil inhibited in a concentration dependent manner cell mobility whereas the adhesion area and the cell circumference were increased. The minoxidil treated cultures had a higher desmosome density compared to parallel control cultures and they expressed the suprabasal keratins 1 and 10 (indicating progress in differentiation) earlier and to a larger extent than the controls. Keratins were revealed immunohistochemically and by two-dimensional polyacrylamide gel electrophoresis. The results suggest that minoxidil supports the differentiation of human keratinocytes.
Bernd
A
A
Abteilung I, Johann Wolfgang Goethe-Universität, Frankfurt/Main, Fed. Rep. of Germany.
Jackel
C
C
Dold
K
K
Görmar
F
F
Theilig
C
C
Breuer
M
M
Ramirez-Bosca
A
A
Bereiter-Hahn
J
J
Holzmann
H
H
D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D003896:Desmosomes / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000187:drug effects* / Q000648:ultrastructure; D007633:Keratins / Q000096:biosynthesis; D008914:Minoxidil / Q000494:pharmacology*
D008914:Minoxidil; D007633:Keratins