Septide but not substance P stimulates inhibitory neurons in guinea-pig ileum.
Recent evidence suggests that [pGlu6,Pro9]substance P-(6-11) (septide) may act on unusual tachykinin receptors. Here, we investigated the mechanisms of action of septide and other agonists acting on tachykinin NK1 receptors, in the guinea-pig ileum. Responses to septide but not to substance P or [Sar9,Met(O2)11]substance P were significantly (P < 0.01) potentiated by 1 microM tetrodotoxin. The data suggest that septide not only acts directly on smooth muscle tachykinin receptors to produce contraction, but also stimulates another receptor subtype on myenteric neurons to release inhibitory substance(s).
Burcher
E
E
School of Physiology and Pharmacology, University of New South Wales, Sydney, Australia.
Stamatakos
C
C
D000818:Animals; D006168:Guinea Pigs; D007082:Ileum / Q000166:cytology / Q000187:drug effects*; D066298:In Vitro Techniques; D009474:Neurons / Q000187:drug effects*; D010446:Peptide Fragments / Q000494:pharmacology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D018040:Receptors, Neurokinin-1 / Q000187:drug effects* / Q000378:metabolism; D013373:Substance P / Q000031:analogs & derivatives* / Q000494:pharmacology*
D010446:Peptide Fragments; D018040:Receptors, Neurokinin-1; C062618:substance P, Sar(9)-Met(O2)(11)-; D013373:Substance P; C054725:septide; D011761:Pyrrolidonecarboxylic Acid