Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli.
Reverse transcriptase from feline immunodeficiency virus (FIV) has been cloned and expressed in Escherichia coli. We have purified this recombinant enzyme and shown that it is a 66-kDa protein that is indistinguishable from virion-derived FIV reverse transcriptase in sensitivity to the 5'-triphosphates of 3'-azido-3'-deoxythymidine and the four 2',3'-dideoxynucleosides. The availability of large quantities of the FIV reverse transcriptase will allow more detailed physical and pharmacological studies.
North
T W
TW
Division of Biological Sciences, University of Montana, Missoula 59812.
Hansen
G L
GL
Zhu
Y
Y
Griffin
J A
JA
Shih
C K
CK
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D016089:Immunodeficiency Virus, Feline / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D014771:Virion / Q000201:enzymology
D004279:DNA, Viral; D011994:Recombinant Proteins; D012194:RNA-Directed DNA Polymerase