Antibodies specific for the neuronal form of the Src protein elicited by an antigenized antibody.
To elicit antibodies directed specifically against the neuron-specific form of the c-src gene product, pp60c-src(+), we used an antigenized antibody comprising a decamer containing the amino acid sequence specific to pp60c-src(+) inserted into the third hypervariable loop of the heavy (H)-chain variable (V)-region. This was used to raise anti-idiotype antibodies reacting with the peptide epitope in rabbits. The antisera reacted with pp60c-src(+), as judged by immune blotting, immunoprecipitation, immune complex kinase assay, and indirect immunofluorescence staining, but did not react with the fibroblast form of the c-src gene product, pp60c-src. Antigenized antibody is a useful approach for producing antibodies able to distinguish between isoforms of the same gene product and specific for the neuronal form of the Src protein.
Rigaudy
P
P
Molecular Biology and Virology Laboratory, Salk Institute, San Diego, CA 92186.
Simon
S
S
Hunter
T
T
Sollazzo
M
M
Billetta
R
R
Zanetti
M
M
Eckhart
W
W
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000235:genetics / Q000276:immunology*; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000918:Antibody Specificity; D001483:Base Sequence; D002460:Cell Line; D004247:DNA; D000939:Epitopes; D005260:Female; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000276:immunology*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D011994:Recombinant Proteins; D004247:DNA; D016392:Proto-Oncogene Proteins pp60(c-src)