Surimi and native codfish contain a common allergen identified as a 63-kDa protein.
We have compared the allergenicity of codfish and surimi (prepared from codfish) by skin testing, specific IgE-RIA, and leukocyte histamine release (LHR) in six fish-allergic patients. Prick tests were positive for codfish and, to a lesser extent, surimi. The percentages of labeled anti-IgE bound to surimi-Sepharose were 1.55 +/- 0.19% and 3-6% with control and patient sera, respectively. Inhibition of the surimi protein-Sepharose IgE-RIA was greatest (80%) at protein concentrations of 13.4 and 408.5 micrograms/ml for codfish and surimi extract, respectively. The allergenic protein was isolated by gel filtration and subjected to SDS-PAGE. The eluate from codfish contained several proteins ranging from 13 to 63 kDa, while the eluate from surimi contained a single 63-kDa protein. It was concluded that surimi contained a single allergenic protein.
Mata
E
E
Laboratoire de Biochimie Nutritionnelle, INSERM U 308, Faculté de Médicine de Nancy, France.
Favier
C
C
Moneret-Vautrin
D A
DA
Nicolas
J P
JP
Han Ching
L
L
Guéant
J L
JL
D000293:Adolescent; D000328:Adult; D000368:Aged; D000485:Allergens / Q000032:analysis* / Q000276:immunology; D000818:Animals; D002648:Child; D002675:Child, Preschool; D002850:Chromatography, Gel; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005396:Fish Products / Q000009:adverse effects / Q000032:analysis; D005399:Fishes / Q000276:immunology*; D005512:Food Hypersensitivity / Q000276:immunology; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D011852:Radioallergosorbent Test; D011863:Radioimmunoassay; D012882:Skin Tests
D000485:Allergens; D007073:Immunoglobulin E