Influence of Tołpa Peat Preparation on the IgE-induced anaphylactic reactions in mice.
The ability of Tołpa Peat Preparation (TPP) to affect anaphylactic sensitization and mast cell secretory function was tested in BALB/c mice treated with TPP orally for 12 days. TPP in the doses of 20 and 50 mg/kg/day reduced histamine release from mouse peritoneal mast cells challenged with anti-IgE or concanavalin A in vitro. The treatment of mice with TPP from day 1 to day 12 of immunization with Ovalbumin (OA) absorbed on aluminium hydroxide gel resulted in a decrease of antigen-induced histamine release from mast cells of these mice in vitro and in decreased IgE antibody level in their sera. TPP introduced into OA-immunized mice showing developed IgE antibody response was less effective in decreasing anaphylactic histamine release from mast cells of these mice. In all experiments low doses of TPP used for oral treatment were more effective than high doses in inhibiting anaphylactic events in the mice.
Wyczółkowska
J
J
Department of Biogenic Amines, Polish Academy of Sciences, Lódź.
Michoń
T
T
Slusarczyk
A
A
Kolago
B
B
Maśliński
C
C
D000596:Amino Acids / Q000494:pharmacology*; D000707:Anaphylaxis / Q000517:prevention & control*; D000818:Animals; D002241:Carbohydrates / Q000494:pharmacology*; D004338:Drug Combinations; D005260:Female; D006636:Histamine Release; D006812:Humic Substances / Q000494:pharmacology*; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar; D012987:Soil; D014574:Uronic Acids / Q000494:pharmacology*
D000596:Amino Acids; D002241:Carbohydrates; D004338:Drug Combinations; D006812:Humic Substances; D012987:Soil; C084029:Tolpa; D014574:Uronic Acids; D007073:Immunoglobulin E; D010047:Ovalbumin