Intermediate filament proteins and epithelial differentiation in the embryonic ovary of the rat.
The development and sexual differentiation of gonads in female rat embryos and fetuses between the ages of 11 and 17 days was studied by immunocytochemical analysis of intermediate filament proteins and laminin by light and electron microscopy. In the 11-day-old pregonadal embryo, the surface epithelial cells in the ventral cortex of the mesonephros contained desmin but not cytokeratin or vimentin. The development of the gonad began on the following day by proliferative growth of the mesonephric surface cells, which like the subepithelial cells soon expressed vimentin in addition to desmin. The differentiation continued by formation of separate epithelial cell clusters, which joined into cords, irregular in shape and size. Desmin disappeared from the cord cells and cytokeratins appeared while vimentin remained in all somatic cell types. Desmin was especially abundant in some stromal cells adjacent to the epithelial tissues. After the segration of the basic ovarian tissues, vimentin and desmin decreased and cytokeratins appeared in the surface epithelial cells. New changes in cytokeratin expression appeared with the differentiation of the embryonic cords in a sex-specific manner with gradual decrease of reactivity for cytokeratin 18. No immunoreaction to the neurofilament proteins was found at the present ages, and the germ cells were negative for intermediate filaments. The results show that desmin is expressed in several primitive ovarian and mesonephric cells even though they are not myogenic.(ABSTRACT TRUNCATED AT 250 WORDS)
Fröjdman
K
K
Laboratory of Electron Microscopy, University of Turku, Finland.
Paranko
J
J
Virtanen
I
I
Pelliniemi
L J
LJ
D000818:Animals; D002454:Cell Differentiation; D003893:Desmin / Q000032:analysis / Q000502:physiology; D004622:Embryo, Mammalian / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000502:physiology; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry; D005260:Female; D007150:Immunohistochemistry; D007381:Intermediate Filament Proteins / Q000032:analysis* / Q000502:physiology; D007633:Keratins / Q000032:analysis / Q000502:physiology; D007797:Laminin / Q000032:analysis / Q000502:physiology; D008297:Male; D010053:Ovary / Q000737:chemistry* / Q000166:cytology / Q000196:embryology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012727:Sex Characteristics; D013737:Testis / Q000737:chemistry / Q000166:cytology / Q000196:embryology; D014746:Vimentin / Q000032:analysis / Q000502:physiology
D003893:Desmin; D007381:Intermediate Filament Proteins; D007797:Laminin; D014746:Vimentin; D007633:Keratins