[Selective determination of different types of gastrointestinal mucins:use of histochemical reagents].
A method, transposed from the mucin histochemical stainings, was proposed to evaluate neutral, acidic and sulphated mucins by spectrocolorimetry. Stainings used were periodic acid/Schiff (PAS), Alcian blue (AB) pH 2.5, and Alcian blue (AB) pH 0.5. Mucin samples were extracted from mucosal scrapings, from intestinal contents of germ-free rats or from commercial pig gastric mucin. A mucin-enriched fraction was obtained and lyophilised and the protein content was determined. Each mucin type was spectrophotometrically analysed after staining and precipitation by slightly modified Carnoy fixative. The histochemical stainings used here, after modification by Carnoy fixative treatment, were, as shown by electrophoretic controls, specific for mucin types contained in these mucins without protein contaminant interference.
Fontaine
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INRA, laboratoire de nutrition et sécurité alimentaire, Jouy-en-Josas, France.
Meslin
J C
JC
D000423:Alcian Blue; D000818:Animals; D004064:Digestive System / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008297:Male; D009077:Mucins / Q000032:analysis*; D010503:Periodic Acid-Schiff Reaction; D051381:Rats; D011916:Rats, Inbred F344; D013053:Spectrophotometry; D013194:Staining and Labeling; D013552:Swine
D007202:Indicators and Reagents; D009077:Mucins; D000423:Alcian Blue