Angiogenic activity of the recombinant hst-1 protein.
The hst-1 transforming gene encodes a protein which belongs to the FGF family of growth factors. We showed previously that a human hst-1 protein produced in silkworm cells has in vitro mitogenic activity to vascular endothelial cells. Here we report effective synthesis of an unfused human hst-1 protein in E. coli and a potent in vivo angiogenic activity of this hst-1 protein by two in vivo assays for angiogenesis, chick chorioallantoic membrane assay and rat cornea assay. The NIH3T3 transformant transfected with the hst-1 gene appeared to develop a highly-vascularized tumor on nude mice. These data showed that the hst-1 protein has an angiogenic activity in vivo as well as in vitro.
Yoshida
T
T
Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.
Ishimaru
K
K
Sakamoto
H
H
Yokota
J
J
Hirohashi
S
S
Igarashi
K
K
Sudo
K
K
Terada
M
M
D000818:Animals; D002642:Chick Embryo; D003315:Cornea / Q000098:blood supply; D051519:Fibroblast Growth Factor 4; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D066298:In Vitro Techniques; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000098:blood supply; D009389:Neovascularization, Pathologic; D011518:Proto-Oncogene Proteins / Q000494:pharmacology*; D051381:Rats; D011994:Recombinant Proteins
C496446:Fgf4 protein, mouse; C496447:Fgf4 protein, rat; D051519:Fibroblast Growth Factor 4; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D005346:Fibroblast Growth Factors