title: Lymphocyte function-associated antigen 1 overexpression and T cell autoreactivity.
abstract: OBJECTIVE
  To determine if DNA methylation inhibitors make T cells autoreactive by inducing lymphocyte function-associated antigen type 1 (LFA-1) (CD11a/CD18) overexpression.
  
  
  METHODS
  T cell clones were treated with 3 distinct DNA methylation inhibitors or were stably transfected with a CD18 cDNA in a mammalian expression vector, and the effects on LFA-1 expression and activation requirements were examined.
  
  
  RESULTS
  LFA-1 overexpression, caused by DNA methylation inhibitors or by transfection, correlates with the development of autoreactivity.
  
  
  CONCLUSIONS
  LFA-1 overexpression may contribute to T cell autoreactivity.
authors.affiliation: University of Michigan, Ann Arbor.
authors.forename: B
authors.initials: B
authors.lastname: Richardson
authors.forename: D
authors.initials: D
authors.lastname: Powers
authors.forename: F
authors.initials: F
authors.lastname: Hooper
authors.forename: R L
authors.initials: RL
authors.lastname: Yung
authors.forename: K
authors.initials: K
authors.lastname: O'Rourke
mesh_terms: D001374:Azacitidine / Q000494:pharmacology; D018821:CD18 Antigens / Q000235:genetics; D004247:DNA / Q000378:metabolism; D018076:DNA, Complementary; D006801:Humans; D008213:Lymphocyte Activation; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D008745:Methylation / Q000187:drug effects; D010957:Plasmids / Q000502:physiology; D011342:Procainamide / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D014162:Transfection; D014466:Ultraviolet Rays
chemical_list: D018821:CD18 Antigens; D018076:DNA, Complementary; D016169:Lymphocyte Function-Associated Antigen-1; D004247:DNA; D011342:Procainamide; D001374:Azacitidine