NMR evidence for the RNA stem-loop structure involved in the transcription attenuation of E. coli trp operon.
High field 1H-NMR studies of a synthetic 21-mer RNA fragment, corresponding to residues +114 to +134 within the trp leader mRNA transcript, have been carried out. Seven well resolved imino proton resonances corresponding to six C-G and one A-U hydrogen bonded base pairs, together with their characteristic NOE patterns can be identified in the NMR spectrum. This experimental result provides direct evidence for the postulated stem-loop secondary structure, 3:4, which has been reported to act as a transcription termination signal for RNA polymerase.
Ramesh
V
V
Department of Biochemistry, University of Leicester, UK.
D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000737:chemistry; D009876:Operon; D012329:RNA, Bacterial / Q000235:genetics / Q000648:ultrastructure; D012333:RNA, Messenger / Q000235:genetics* / Q000648:ultrastructure; D014158:Transcription, Genetic
D009843:Oligoribonucleotides; D012329:RNA, Bacterial; D012333:RNA, Messenger