title: Insulin-like growth factor and growth hormone receptor in nephrotic rats.
abstract: In an attempt to elucidate the mechanism of growth retardation in the nephrotic syndrome, specific serum and hepatic growth factors were measured in Sprague-Dawley rats in which nephrotic syndrome was produced by administration of puromycin (1.5 mg.100 g body wt-1.day-1) for 12 days. On the 13th day, the results of these nephrotic animals were compared with those of an equal number of pair-fed and control animals: the mean dietary intake of the nephrotic group was 71% that of the control group (P < 0.001). Serum insulin-like growth factor (IGF) binding protein-3 was significantly reduced (P < 0.005) in the nephrotic rats, compared with the pair-fed and the control groups. Hepatic IGF-I mRNA in the nephrotic rats averaged 36% that of control (P < 0.001) and 46% that of the pair-fed animals (P < 0.001). Hepatic growth hormone receptor (GHr) mRNA in the nephrotic rats averaged 19% of that of the control (P < 0.001) and 27% of that of the pair-fed rats (P < 0.001). These results indicate that the growth retardation of the nephrotic rats may be associated with the significant decrease in IGF-I mRNA and reduction in GHr mRNA.
authors.affiliation: Department of Pediatrics, Children's Medical Center, Medical College of Virginia, Richmond.
authors.forename: M A
authors.initials: MA
authors.lastname: Thabet
authors.forename: A
authors.initials: A
authors.lastname: Challa
authors.forename: W
authors.initials: W
authors.lastname: Chan
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.forename: R L
authors.initials: RL
authors.lastname: Hintz
authors.forename: J C
authors.initials: JC
authors.lastname: Chan
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism; D005260:Female; D018969:Insulin-Like Growth Factor Binding Proteins; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D008099:Liver / Q000378:metabolism; D009404:Nephrotic Syndrome / Q000097:blood* / Q000139:chemically induced / Q000378:metabolism; D011691:Puromycin; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011986:Receptors, Somatotropin / Q000235:genetics / Q000378:metabolism*; D013002:Somatomedins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D011986:Receptors, Somatotropin; D013002:Somatomedins; D011691:Puromycin; D007334:Insulin-Like Growth Factor I