Binding characteristics of recombinant human endothelin receptors ETA and ETB expressed in baby hamster kidney cells.
Expression constructs for human endothelin (ET) receptors ETA and ETB were made by subcloning the corresponding coding sequences into the pMPSV/CMV vector. They were used together with plasmids bearing resistances to puromycin and hygromycin for transfection of baby hamster kidney cells. Culture in the presence of both antibiotics allowed the selection of cell lines stably expressing one receptor or the other. Competitive binding experiments using ET-1 and ET-3 showed the typical isopeptide-selective and non-isopeptide-selective profiles for recombinant ETA and ETB, respectively.
Hechler
U
U
Research Laboratories of Schering AG, Berlin, Germany.
Becker
A
A
Haendler
B
B
Schleuning
W D
WD
D000818:Animals; D001667:Binding, Competitive / Q000187:drug effects; D002460:Cell Line; D002934:Cinnamates; D006224:Cricetinae; D005057:Eukaryotic Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D007457:Iodine Radioisotopes; D007668:Kidney / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D017466:Receptors, Endothelin / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection
D002934:Cinnamates; D007457:Iodine Radioisotopes; D017466:Receptors, Endothelin; D011994:Recombinant Proteins; D006921:Hygromycin B; C026273:hygromycin A; D011691:Puromycin