title: Enhanced detection of antibodies to hepatitis C virus by use of a third-generation recombinant immunoblot assay.
abstract: Fifty-seven sera with indeterminate results by the second generation RIBA (RIBA 2) for confirmation of hepatitis C virus (HCV) enzyme linked immunoassay (ELISA) reactivity were tested by the new third generation RIBA (RIBA 3). Thirty three (57.9%) displayed reactivity for at least one other band and were therefore classified as positive; two became negative and 22 (38%) remained indeterminate. The incidence of HCV viremia, as determined by the RNA polymerase chain reaction (PCR), was 75% for the latter sera. The data show that it is important to subject RIBA 3 indeterminate samples to PCR.
authors.affiliation: Service des Maladies due Foie et de L'Appareil Digestif, Hôpital de Bicêtre, Le Kremlin Bicêtre, France.
authors.forename: C
authors.initials: C
authors.lastname: Buffet
authors.forename: N
authors.initials: N
authors.lastname: Charnaux
authors.forename: P
authors.initials: P
authors.lastname: Laurent-Puig
authors.forename: S
authors.initials: S
authors.lastname: Chopineau
authors.forename: J P
authors.initials: JP
authors.lastname: Quichon
authors.forename: M J
authors.initials: MJ
authors.lastname: Briantais
authors.forename: E
authors.initials: E
authors.lastname: Dussaix
mesh_terms: D000293:Adolescent; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D006508:Hepatitis Antibodies / Q000097:blood*; D006526:Hepatitis C / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D018937:Hepatitis C Antibodies; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D008297:Male; D016133:Polymerase Chain Reaction; D012698:Serologic Tests; D014766:Viremia / Q000175:diagnosis / Q000276:immunology / Q000821:virology
chemical_list: D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies