Micronuclei and 3AB index in X-irradiated human lymphocytes in G0 and G1 phases.
We applied the cytokines-block micronucleus assay to observe the radiobiological response of human lymphocytes after X-ray treatment in the G0 and G1 phases. In addition, we used 3-aminobenzamide (3AB) to measure the 3AB index in the two phases. The experimental results show that at 2 Gy the MN yield and the 3AB index are dependent on the cell phase and show considerable inter-individual variability. The radiation-induced MN frequency obtained for 33 subjects is 0.470 +/- 0.063 for the G0 phase and 0.689 +/- 0.139 for the G1 phase; the 3AB index values are 0.326 +/- 0.144 and 0.067 +/- 0.058 for G0 and G1 phases, respectively. At the individual level, the 3AB index for the G1 phase correlates inversely with the cytogenetic effects observed in that phase. We discuss the possibility of applying the MN test combined with the 3AB index to lymphocytes at different phases to study the individual response to radiation (individual radiosensitivity).
Catena
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C
AMB-EFF, ENEA CRE Casaccia, Rome, Italy.
Villani
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P
Conti
D
D
Righi
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E
D000328:Adult; D001549:Benzamides / Q000633:toxicity*; D004260:DNA Repair / Q000187:drug effects; D004307:Dose-Response Relationship, Radiation; D016193:G1 Phase / Q000187:drug effects / Q000528:radiation effects; D014644:Genetic Variation; D006801:Humans; D007399:Interphase / Q000187:drug effects / Q000528:radiation effects*; D008214:Lymphocytes / Q000187:drug effects / Q000528:radiation effects*; D015162:Micronucleus Tests; D008875:Middle Aged; D011836:Radiation Tolerance / Q000235:genetics*; D011838:Radiation-Sensitizing Agents / Q000633:toxicity*; D016192:Resting Phase, Cell Cycle / Q000187:drug effects / Q000528:radiation effects
D001549:Benzamides; D011838:Radiation-Sensitizing Agents; C025160:3-aminobenzamide