High performance liquid chromatographic profiling of tryptophan and related indoles in body fluids and tissues of carcinoid patients.
A high performance liquid chromatographic method with quaternary gradient elution and fluorometric detection was developed for profiling of tryptophan (TRP), 5-hydroxytryptophan, serotonin (5-HT) and 5-hydroxyindole-3-acetic acid (5-HIAA) in urine, platelet-rich plasma and (tumour) tissue of patients with carcinoid tumours. Prior to injection, urine samples were diluted and filtered. Platelet-rich plasma and tissue homogenates were prepurified by C18 solid phase extraction. Detection limits were approx. 2 pmol. Results of urinary 5-HT and 5-HIAA compared favourably with those of single component analyses. No consistent diurnal variations were found for TRP, 5-HT and 5-HIAA in 12-h urine samples from 15 healthy adults. Abstinence of 5-HT-rich foods reduced urinary levels of 5-HT and 5-HIAA. C18 extraction of indoles from protein-containing matrices was studied in platelet-rich plasma. Although time-consuming and complicated for daily routine use, the present approach offers particular advantages over single component analyses in the study of TRP metabolism in patients with carcinoid tumours.
Kema
I P
IP
Central Laboratory for Clinical Chemistry, University and University Hospital of Groningen, Netherlands.
Schellings
A M
AM
Hoppenbrouwers
C J
CJ
Rutgers
H M
HM
de Vries
E G
EG
Muskiet
F A
FA
D006916:5-Hydroxytryptophan / Q000032:analysis / Q000097:blood / Q000652:urine; D001792:Blood Platelets / Q000737:chemistry; D001826:Body Fluids / Q000737:chemistry*; D002276:Carcinoid Tumor / Q000097:blood / Q000737:chemistry* / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002940:Circadian Rhythm; D004032:Diet; D006801:Humans; D006897:Hydroxyindoleacetic Acid / Q000032:analysis / Q000097:blood / Q000652:urine; D007211:Indoles / Q000032:analysis* / Q000097:blood / Q000652:urine; D011786:Quality Control; D015203:Reproducibility of Results; D012701:Serotonin / Q000032:analysis / Q000097:blood / Q000652:urine; D014364:Tryptophan / Q000032:analysis* / Q000097:blood / Q000652:urine
D007211:Indoles; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D014364:Tryptophan; D006916:5-Hydroxytryptophan