title: The conformation of the sialyl Lewis X ligand changes upon binding to E-selectin.
abstract: High-field NMR spectroscopy has been used to study the complex formed by the tetrasaccharide sialyl Lewis X and its receptor, E-selectin. Transferred NOEs demonstrate a specific interaction between the protein and ligand and enable measurement of the dissociation constant for the complex to be between approximately 1.1 and 2.0 mM. Differences between Overhauser spectra for free and bound sialyl Lewis X highlight a conformational change upon binding. This can be pinpointed to a change in the torsion angle of the glycosidic link between the sialyl and galactosyl residues and used to select a likely "bound" conformation from four low-energy species. Docking the bound form of sialyl Lewis X onto a model of the lectin domain of E-selectin suggests that the conformational change upon binding results primarily from steric interactions.
authors.affiliation: Department of Biomolecular Structure, Glaxo Research and Development, Greenford, Middlesex, U.K.
authors.forename: R M
authors.initials: RM
authors.lastname: Cooke
authors.forename: R S
authors.initials: RS
authors.lastname: Hale
authors.forename: S G
authors.initials: SG
authors.lastname: Lister
authors.forename: G
authors.initials: G
authors.lastname: Shah
authors.forename: M P
authors.initials: MP
authors.lastname: Weir
mesh_terms: D002240:Carbohydrate Sequence; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D003198:Computer Simulation; D019040:E-Selectin; D006801:Humans; D066298:In Vitro Techniques; D016256:Lewis X Antigen / Q000737:chemistry*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D011994:Recombinant Proteins
chemical_list: D015815:Cell Adhesion Molecules; D019040:E-Selectin; D016256:Lewis X Antigen; D008024:Ligands; D011994:Recombinant Proteins