title: Rapid GC-MS confirmation of urinary amphetamine and methamphetamine as their propylchloroformate derivatives.
abstract: Amphetamine and methamphetamine are extracted from 200 microL of urine into an organic solvent containing propylchloroformate. The amines immediately react to form propylcarbamate derivatives. The aqueous phase is removed, and a portion of the organic phase is analyzed by gas chromatography-mass spectrometry. Either the deuterated analogues or N-propylamphetamine internal standards give acceptable results. For day-to-day precision, coefficients of variation in the range of 6-10% are found. The method is linear to 10,000 ng/mL. Limits of quantitation and detection are 50 and 5 ng/mL, respectively, when using N-propylamphetamine as the internal standard. Extracts are stable at room temperature for at least 60 days. There is no interference from other amphetamine drugs.
authors.affiliation: Department of Biochemistry, St. Boniface General Hospital, Winnipeg, MB, Canada.
authors.forename: R
authors.initials: R
authors.lastname: Meatherall
mesh_terms: D000661:Amphetamine / Q000652:urine*; D004809:Ephedrine / Q000652:urine; D005563:Formic Acid Esters / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008694:Methamphetamine / Q000031:analogs & derivatives* / Q000652:urine*; D010645:Phentermine / Q000652:urine
chemical_list: D005563:Formic Acid Esters; D008694:Methamphetamine; D010645:Phentermine; D000661:Amphetamine; D004809:Ephedrine