Modulation of contractile proteins in embryonic and fetal chick cardiac cells by phorbol ester, gamma-interferon, 5-azacytidine and diacylglycerols.
We studied changes in the concentration of tropomyosin, actin, desmin and vimentin in cultured myocardiocytes from Hamburger and Hamilton's stages 29 and 39 chick embryos (HH29 and HH39) (1), treated with 12-o-tetradecanoyl-phorbol-13-acetate (TPA), 5-azacytidine (AZA), gamma interferon (INF) and diacylglycerols (DAG). In embryonic myocardiocytes at HH29, the first three agents modified the intracellular distribution of the thin filament proteins tropomyosin and actin, increasing their cytoplasmic concentration and decreasing their cytoskeletal concentration. The concentration of the intermediate filament proteins desmin and vimentin increased in both subcellular fractions after treatment with these drugs. In fetal myocardiocytes at HH39, total protein content decreased after treatment with these drugs. Cytoplasmic and cytoskeletal concentrations of actin and tropomyosin decreased to different degrees after treatment with TPA, AZA or DAG in HH39 myocardiocytes. TPA, AZA and DAG decreased desmin in the cytoplasmic and cytoskeletal fractions. These findings suggest that the drugs tested alter the normal protein composition in cultured myocardiocytes, and have different effects depending on the developmental stage in which the embryo is treated.
Muros
M A
MA
Department of Morphological Sciences, School of Medicine, University of Granada, Spain.
Aránega
A E
AE
Vélez
C
C
Melguizo
C
C
Alvarez
L
L
Aránega
A
A
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology*; D002642:Chick Embryo; D003593:Cytoplasm / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D004075:Diglycerides / Q000494:pharmacology*; D005333:Fetus / Q000502:physiology; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D007371:Interferon-gamma / Q000494:pharmacology*; D009124:Muscle Proteins / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D009200:Myocardial Contraction / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D004075:Diglycerides; D009124:Muscle Proteins; D012333:RNA, Messenger; D007371:Interferon-gamma; D001374:Azacitidine; D013755:Tetradecanoylphorbol Acetate