title: A neutral sugar is responsible for serovar specificity of the antigenic determinant of Leptospira interrogans serovar canicola.
abstract: To provide information on the chemical structures of antigenic determinants of leptospira, glycolipids of Leptospira interrogans serovar canicola strain Hond Utrecht IV (Ut-IV) and its antigenic variant selected in the presence of a serovar-specific monoclonal antibody were compared physicochemically. Gas-liquid chromatography-mass spectrometry analysis revealed that the glycolipid of Ut-IV contained 6 neutral sugar species; rhamnose, mannose, galactose, glucose, and unknown sugars III and IV, in addition to unknown sugars I and II that had been previously reported. On the other hand, the glycolipid of the variant lacked unknown sugar III, suggesting that this sugar is responsible for the serovar-specific antigenic determinant.
authors.affiliation: Department of Hygiene and Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kashiwase
authors.forename: E
authors.initials: E
authors.lastname: Ono
authors.forename: R
authors.initials: R
authors.lastname: Yanagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Shimizu
authors.forename: H
authors.initials: H
authors.lastname: Kida
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology; D002241:Carbohydrates / Q000032:analysis; D000939:Epitopes / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D014644:Genetic Variation; D006017:Glycolipids / Q000737:chemistry* / Q000276:immunology; D007921:Leptospira interrogans / Q000145:classification / Q000276:immunology*; D051379:Mice / Q000276:immunology; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D002241:Carbohydrates; D000939:Epitopes; D006017:Glycolipids; D009844:Oligosaccharides