title: Isozyme-specific inhibition of protein kinase C by RNA aptamers.
abstract: In vitro selection technology has been used to purify RNA aptamers from a random sequence pool that can bind to, and specifically inhibit, protein kinase C beta II. Two of the selected RNA aptamers bind to this isozyme of protein kinase C with nanomolar affinities and inhibit activation with unprecedented selectivity; the highly related, alternatively spliced beta I isozyme, which differs by 23 residues, is inhibited with 1 order of magnitude lower potency; the next most similar isozyme, alpha, shows no detectable inhibition. The production of isozyme-specific inhibitors of protein kinase C opens the possibilities for dissecting the roles of specific protein kinase Cs in the myriad of intracellular signalling pathways.
authors.affiliation: Department of Chemistry, Indiana University, Bloomington 47405.
authors.forename: R
authors.initials: R
authors.lastname: Conrad
authors.forename: L M
authors.initials: LM
authors.lastname: Keranen
authors.forename: A D
authors.initials: AD
authors.lastname: Ellington
authors.forename: A C
authors.initials: AC
authors.lastname: Newton
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D007527:Isoenzymes / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D012326:RNA Splicing
chemical_list: D017931:DNA Primers; D007527:Isoenzymes; D012313:RNA; D011493:Protein Kinase C