title: Polycos vectors: a system for packaging filamentous phage and phagemid vectors using lambda phage packaging extracts.
abstract: A new class of hybrid vectors, 'polycos' vectors, incorporate a phage lambda cos site and filamentous phage origin to allow high-efficiency cloning via in vitro lambda packaging extracts. The head-filling mechanism of cos site recognition by lambda packaging proteins permits concatemers of several of these small cos-containing vectors to be packaged per phage particle. Excision of vector monomers after infection is accomplished via a lambda ZAP-like M13 excision process. This system has the advantage of adapting high-efficiency lambda packaging extracts to M13 and phagemid cloning vectors.
authors.affiliation: Stratagene, La Jolla, CA 92037.
authors.forename: M A
authors.initials: MA
authors.lastname: Alting-Mees
authors.forename: J M
authors.initials: JM
authors.lastname: Short
mesh_terms: D017104:Bacteriophage M13; D010582:Bacteriophage lambda; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D003360:Cosmids; D004262:DNA Restriction Enzymes; D000939:Epitopes; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins
chemical_list: D000939:Epitopes; D011993:Recombinant Fusion Proteins; D004262:DNA Restriction Enzymes