Cloning and characterization of cDNA coding for a new allergen from the house dust mite, Dermatophagoides farinae.
A cDNA clone encoding a new allergen from the Dermatophagoides farinae cDNA lambda gt11 library was isolated and sequenced. There was no amino acid sequence homology with other known allergens. The gene product, beta-galactosidase fusion protein, of the truncated cDNA on blot reacted with IgE in 13 of 43 sera from patients allergic to mites. The affinity-purified fusion protein had a potent ability to release histamine from washed blood cells of the mite-allergic patients. Human specific IgE eluted from the fusion protein band on blots recognized a 39-kD component on blots of a mite body extract.
Aki
T
T
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
Ono
K
K
Paik
S Y
SY
Wada
T
T
Jyo
T
T
Shigeta
S
S
Murooka
Y
Y
Oka
S
S
D000485:Allergens / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D039741:Antigens, Dermatophagoides; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004391:Dust; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology; D006636:Histamine Release; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology; D008925:Mites / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology
D000485:Allergens; D039741:Antigens, Dermatophagoides; D018076:DNA, Complementary; D004391:Dust; D006023:Glycoproteins; D011994:Recombinant Proteins; D007073:Immunoglobulin E