title: SITEVIDEO: a computer system for functional site analysis and recognition. Investigation of the human splice sites.
abstract: We developed the computer system SITEVIDEO for analysis and recognition of the functional sites in DNA and RNA molecules. It reveals contextual features essential for site function and thus enable the user to design efficient methods for recognition of the functional sites. We mainly considered only quantitative characteristics reflecting the uneven distribution of oligonucleotides in the sequences of functional sites of interest. The approach suggested makes use of available information about the hierarchical organization of the functional sites, and ensures highly precise prediction of the sites. The present analysis is concerned with the human donor and acceptor splice sites. A method for recognizing these sites in the sequences with an accuracy of approximately 90% was developed.
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk.
authors.forename: A E
authors.initials: AE
authors.lastname: Kel
authors.forename: M P
authors.initials: MP
authors.lastname: Ponomarenko
authors.forename: E A
authors.initials: EA
authors.lastname: Likhachev
authors.lastname: Orlov YuL
authors.forename: I V
authors.initials: IV
authors.lastname: Ischenko
authors.forename: L
authors.initials: L
authors.lastname: Milanesi
authors.forename: N A
authors.initials: NA
authors.lastname: Kolchanov
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D003199:Computer Systems; D016384:Consensus Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015894:Genome, Human; D006801:Humans; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics / Q000378:metabolism; D012326:RNA Splicing / Q000235:genetics*; D012984:Software; D012985:Software Design
chemical_list: D012313:RNA; D004247:DNA