Magnetic resonance microscopy of embryonic cell lineages and movements.
Key events in vertebrate embryogenesis are difficult to observe in many species. High-resolution magnetic resonance imaging was used to follow cell movements and lineages in developing frog embryos. A single cell was injected at the 16-cell stage with a contrast agent, based on the gadolinium chelate gadolinium-diethylenetriamine pentaacetic acid-dextran. The labeled progeny cells could be followed uniquely in three-dimensional magnetic resonance images, acquired from the embryo over several days. The results show that external ectodermal and internal mesodermal tissues extend at different rates during amphibian gastrulation and neurulation.
Jacobs
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Division of Biology, Beckman Institute, California Institute of Technology, Pasadena 91125.
Fraser
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D000818:Animals; D001755:Blastocyst / Q000166:cytology; D001757:Blastomeres / Q000166:cytology*; D003911:Dextrans; D004475:Ectoderm / Q000166:cytology; D004625:Embryo, Nonmammalian / Q000166:cytology*; D004626:Embryology / Q000379:methods*; D005682:Gadolinium; D019786:Gadolinium DTPA; D005775:Gastrula / Q000166:cytology*; D008279:Magnetic Resonance Imaging; D008648:Mesoderm / Q000166:cytology; D008853:Microscopy; D009024:Morphogenesis; D004369:Pentetic Acid / Q000031:analogs & derivatives; D014982:Xenopus laevis / Q000196:embryology
D003911:Dextrans; C075783:dextran-(gadolinium-DTPA)15; D004369:Pentetic Acid; D005682:Gadolinium; D019786:Gadolinium DTPA