title: Mechanism of initial attachment of cells derived from human bone to commonly used prosthetic materials during cell culture.
abstract: The suitability of polymeric biomaterials as surfaces for the attachment and growth of cells has often been investigated in cell culture. In this study the contribution that serum fibronectin (Fn) or vitronectin (Vn) make to the attachment and spreading of cells cultured from explanted human bone (bone-derived cells) during the first 90 min of culture was determined for metallic and ceramic surfaces. The requirement for Fn or Vn for attachment and spreading of bone-derived cells onto stainless steel 316 (SS), titanium (Ti) and alumina (Al2O3) and to polyethyleneterephthalate (PET) was directly tested by selective removal of Fn or Vn from the serum prior to addition to the culture medium. Attachment and spreading of bone-derived cells onto SS, Ti and Al2O3 surfaces were reduced by 73-83% when the cells were seeded in medium containing serum from which the Vn had been removed. Cell attachment and spreading on these surfaces when seeded in medium containing Fn-depleted serum (which contained Vn) were not reduced to the same extent as in the medium containing Vn-depleted serum. The bone-derived cells failed to attach to the surfaces to the same extent when seeded in medium containing serum depleted of both Vn and Fn. Our results show that for human bone-derived cells, the attachment and spreading of cells onto SS, Ti and Al2O3 as well as PET during the first 90 min of a cell culture attachment assay are a function of adsorption of serum Vn onto the surface.
authors.affiliation: School of Pathology, University of New South Wales, Kensington, Australia.
authors.forename: C R
authors.initials: CR
authors.lastname: Howlett
authors.forename: M D
authors.initials: MD
authors.lastname: Evans
authors.forename: W R
authors.initials: WR
authors.lastname: Walsh
authors.forename: G
authors.initials: G
authors.lastname: Johnson
authors.forename: J G
authors.initials: JG
authors.lastname: Steele
mesh_terms: D000537:Aluminum Oxide / Q000737:chemistry; D001672:Biocompatible Materials; D001842:Bone and Bones / Q000166:cytology*; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000097:blood / Q000494:pharmacology*; D005353:Fibronectins / Q000097:blood / Q000494:pharmacology; D006023:Glycoproteins / Q000097:blood / Q000494:pharmacology; D006801:Humans; D008856:Microscopy, Fluorescence; D011093:Polyethylene Terephthalates / Q000737:chemistry; D013232:Steel / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D019096:Vitronectin
chemical_list: D001672:Biocompatible Materials; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D006023:Glycoproteins; D011093:Polyethylene Terephthalates; D019096:Vitronectin; D013232:Steel; D014025:Titanium; D000537:Aluminum Oxide