title: Pharmacology of the SV channel in the vacuolar membrane of Chenopodium rubrum suspension cells.
abstract: Single channel performance and deactivation currents have been analyzed in the presence of cation channel blockers to reveal pharmacological properties of the slow-activating (SV) cation-selective ion channel in the vacuolar membrane (tonoplast) isolated from suspension cells of Chenopodium rubrum L. At a holding potential of -100 mV, the SV channel showed half-maximal inhibition with 20 mM tetraethylammonium (TEA), 7 microM 9-amino-acridine, 6 microM (+)-tubocurarine, 300 nM quinacrine, and 35 microM quinine, respectively. The SV channel is also blocked by charybdotoxin (20 nM at -80 mV) but not by apamine. 9-Amino-acridine, (+)-tubocurarine and quinacrine act in a voltage-dependent fashion, binding to the open channel and to different sites along the transmembrane voltage profile according to Woodhull (J. Gen. Physiol. 61:687-708, 1973). No binding site could be specified for charybdotoxin, which binds to the closed channel, and for quinine. Except for quinine, all tested blockers were effective only if added to the cytoplasmic side of the tonoplast. A structural relationship between the SV channel and Maxi-K channels in animal systems is inferred.
authors.affiliation: Institut für Allgemeine Botanik und Pfanzenphysiologie, Justus-Liebig-Universität, Giessen, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Weiser
authors.forename: F W
authors.initials: FW
authors.lastname: Bentrup
mesh_terms: D000818:Animals; D002412:Cations; D018999:Charybdotoxin; D007425:Intracellular Membranes / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D007473:Ion Channels / Q000032:analysis / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D059828:Plant Cells; D018521:Plant Physiological Phenomena; D011796:Quinacrine / Q000494:pharmacology; D011803:Quinine / Q000494:pharmacology; D012604:Scorpion Venoms / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014403:Tubocurarine / Q000494:pharmacology
chemical_list: D002412:Cations; D007473:Ion Channels; D012604:Scorpion Venoms; D018999:Charybdotoxin; D011803:Quinine; D011796:Quinacrine; D013755:Tetradecanoylphorbol Acetate; D014403:Tubocurarine