title: Sequence and functional expression of an amphibian water channel, FA-CHIP: a new member of the MIP family.
abstract: A new member of the family of water channel proteins (aquaporin-CHIP) related to the major intrinsic protein (MIP) family is described. The cDNA coding for this amphibian CHIP was cloned from frog (Rana esculenta) urinary bladder, a model for the kidney collecting duct, using a RT-PCR cloning strategy. The encoded protein, designated FA-CHIP (frog aquaporin-CHIP), shows 77.4%, 42.4% and 35.6% identity with the three proteins now referred to as the aquaporins of the MIP family, i.e., human CHIP28, WCH-CD and gamma-TIP, respectively. Xenopus leavis injected with FA-CHIP cRNA exhibited a marked increase of the osmotic water permeability.
authors.affiliation: Département de Biologie Cellulaire et Moléculaire, CEA Saclay, Gif-sur-Yvette, France.
authors.forename: L
authors.initials: L
authors.lastname: Abrami
authors.forename: M
authors.initials: M
authors.lastname: Simon
authors.forename: G
authors.initials: G
authors.lastname: Rousselet
authors.forename: V
authors.initials: V
authors.lastname: Berthonaud
authors.forename: J M
authors.initials: JM
authors.lastname: Buhler
authors.forename: P
authors.initials: P
authors.lastname: Ripoche
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D051398:Aquaporin 1; D020346:Aquaporins; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification* / Q000378:metabolism; D007473:Ion Channels / Q000235:genetics*; D008969:Molecular Sequence Data; D011893:Rana esculenta; D001743:Urinary Bladder / Q000378:metabolism
chemical_list: D020346:Aquaporins; D018076:DNA, Complementary; D007473:Ion Channels; D051398:Aquaporin 1