title: Antibodies specific for the neuronal form of the Src protein elicited by an antigenized antibody.
abstract: To elicit antibodies directed specifically against the neuron-specific form of the c-src gene product, pp60c-src(+), we used an antigenized antibody comprising a decamer containing the amino acid sequence specific to pp60c-src(+) inserted into the third hypervariable loop of the heavy (H)-chain variable (V)-region. This was used to raise anti-idiotype antibodies reacting with the peptide epitope in rabbits. The antisera reacted with pp60c-src(+), as judged by immune blotting, immunoprecipitation, immune complex kinase assay, and indirect immunofluorescence staining, but did not react with the fibroblast form of the c-src gene product, pp60c-src. Antigenized antibody is a useful approach for producing antibodies able to distinguish between isoforms of the same gene product and specific for the neuronal form of the Src protein.
authors.affiliation: Molecular Biology and Virology Laboratory, Salk Institute, San Diego, CA 92186.
authors.forename: P
authors.initials: P
authors.lastname: Rigaudy
authors.forename: S
authors.initials: S
authors.lastname: Simon
authors.forename: T
authors.initials: T
authors.lastname: Hunter
authors.forename: M
authors.initials: M
authors.lastname: Sollazzo
authors.forename: R
authors.initials: R
authors.lastname: Billetta
authors.forename: M
authors.initials: M
authors.lastname: Zanetti
authors.forename: W
authors.initials: W
authors.lastname: Eckhart
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000235:genetics / Q000276:immunology*; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000918:Antibody Specificity; D001483:Base Sequence; D002460:Cell Line; D004247:DNA; D000939:Epitopes; D005260:Female; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000276:immunology*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000235:genetics / Q000276:immunology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology
chemical_list: D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D011994:Recombinant Proteins; D004247:DNA; D016392:Proto-Oncogene Proteins pp60(c-src)