title: Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity.
abstract: Two molecular mechanisms of T cell-mediated cytotoxicity, one perforin-based, the other Fas-based, have been demonstrated. To determine the extent of their contribution to T cell-mediated cytotoxicity, a range of effector cells from normal control or perforin-deficient mice were tested against a panel of target cells with various levels of Fas expression. All cytotoxicity observed was due to either of these mechanisms, and no third mechanism was detected. Thus, the perforin- and Fas-based mechanisms may account for all T cell-mediated cytotoxicity in short-term in vitro assays.
authors.affiliation: Department of Pathology, University of Zürich, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Kägi
authors.forename: F
authors.initials: F
authors.lastname: Vignaux
authors.forename: B
authors.initials: B
authors.lastname: Ledermann
authors.forename: K
authors.initials: K
authors.lastname: Bürki
authors.forename: V
authors.initials: V
authors.lastname: Depraetere
authors.forename: S
authors.initials: S
authors.lastname: Nagata
authors.forename: H
authors.initials: H
authors.lastname: Hengartner
authors.forename: P
authors.initials: P
authors.lastname: Golstein
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D015759:Ionomycin / Q000494:pharmacology; D007939:Leukemia L1210; D007959:Lymphocyte Culture Test, Mixed; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D019014:fas Receptor
chemical_list: D000954:Antigens, Surface; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D019014:fas Receptor; D003208:Concanavalin A; D054353:Perforin; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate