Separation of hematopoietic progenitor cells from human umbilical cord blood.
Human umbilical cord blood (HUCB) is a rich source of hematopoietic progenitor cells (HPC). Nevertheless, it has been previously reported that attempts to enrich for HPC resulted in substantial loss of progenitor cells. We have developed a cell processing technique for HUCB using 3% gelatin sedimentation and the AIS MicroCELLectorSBA and CD34. By this technique, we were able to deplete red blood cells (RBC) and obtain a partial T cell depletion, while maintaining 70-90% of the HPC. The results indicate that it may be possible to manipulate cord blood without significant loss of HPC, thus enhancing its utility for transplantation.
Nagler
A
A
Department of Bone Marrow Transplantation, Hadassah University, Jerusalem, Israel.
Peacock
M
M
Tantoco
M
M
Lamons
D
D
Okarma
T B
TB
Okrongly
D A
DA
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018952:Antigens, CD34; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D005312:Fetal Blood / Q000166:cytology*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation; D007231:Infant, Newborn; D019089:Stem Cell Factor
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018952:Antigens, CD34; D016298:Hematopoietic Cell Growth Factors; D019089:Stem Cell Factor