[Analysis of ovalbumin-specific T cell lines established from patients with hen egg allergy].
Ten ovalbumin (OVA)-specific T cell lines (TCLs) were established from peripheral blood mononuclear cells of 6 patients with hen egg allergy, and the antigen recognition of these TCLs was characterized. Two OVA epitopes were determined by use of 3 synthetic OVA peptides which have been known as murine T cell epitopes. Blocking of antigen-specific T cell proliferation by anti-HLA class II monoclonal antibodies suggest that all 3 HLA class II molecules could act as restriction elements for T cell recognition of OVA. This is the first demonstration of OVA epitopes recognized by T cells in patients with hen egg allergy, as far as we know.
Katsuki
T
T
Department of Pediatrics, Chiba University School of Medicine.
Shimojo
N
N
Honma
K
K
Kohno
Y
Y
Niimi
H
H
Coligan
J E
JE
Tsunoo
H
H
D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D004531:Eggs; D000939:Epitopes / Q000276:immunology; D005260:Female; D005512:Food Hypersensitivity / Q000276:immunology*; D006801:Humans; D007223:Infant; D008297:Male; D010047:Ovalbumin / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D000939:Epitopes; D010047:Ovalbumin