The influence of antigen digestion on orally induced immunity and tolerance.
1. Pepsin-digested form of OVA has approximately the same molecular weight as native OVA, but differs in charge and isoelectric focusing point. 2. Pepsin-treated OVA retains most of the native OVA T- and B-cell determinants. 3. Delayed-type hypersensitivity response is suppressed when pepsin-treated OVA is administered i.p. to naive mice. 4. Cimetidine treatment of mice prior to ingestion of OVA shows decreased tolerance, while oral administration of pepsin-treated OVA to cimetidine-treated mice leads to a complete immune unresponsiveness; this suggests an important role for gastric digestion in orally induced immune tolerance.
Jain
S L
SL
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, OH 45267-0524, USA.
Michael
J G
JG
D000284:Administration, Oral; D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000378:metabolism*; D002927:Cimetidine / Q000494:pharmacology; D004063:Digestion; D005260:Female; D005750:Gastric Juice / Q000187:drug effects / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006968:Hypersensitivity, Delayed; D007108:Immune Tolerance; D018928:Immunity, Mucosal; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D010434:Pepsin A; D010581:Peyer's Patches / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D002927:Cimetidine; D010047:Ovalbumin; D010434:Pepsin A