Application of cationic probes for the ultrastructural localization of proteoglycans in basement membranes.
The application of cationic probes for the ultrastructural detection of proteoglycans in basement membranes is reviewed. Proteoglycans are highly negatively charged macromolecules due to their glycosaminoglycan side chains. The interaction of cationic probes with proteoglycans is of an electrostatic nature. Methods are discussed to increase the specificity of probes for proteoglycans. The use of phthalocyanin-like dyes such as Cuprolinic blue, according to the critical electrolyte concentration method, results in a selective staining of proteoglycans. Enzymatic or chemical digestions, however, should be done to validate the proteoglycan nature of the dye-positive granules/filaments, and to establish the class of proteoglycan. The value of cationic probes in basement membrane research on development and pathology is discussed. The potential for deducting molecular information from the ultrastructural appearance of stained proteoglycans is indicated.
van Kuppevelt
T H
TH
Department of Biochemistry, University of Nijmegen, The Netherlands.
Veerkamp
J H
JH
D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure*; D002412:Cations; D004396:Coloring Agents; D006801:Humans; D011509:Proteoglycans / Q000648:ultrastructure*; D013194:Staining and Labeling / Q000379:methods
D002412:Cations; D004396:Coloring Agents; D011509:Proteoglycans