title: A simple method for isolating alpha 2 macroglobulin-cytokine complexes.
abstract: There is the need for a simple, effective procedure for separating alpha 2 macroglobulin-cytokine complexes from free cytokine in order that the nature and possible immunological significance of cytokine-binding by alpha 2 macroglobulin (alpha 2-M) might be further investigated. This presentation describes a method which exploits the presence of zinc-binding sites on alpha 2-M which permit the isolation of complexes from other proteins by zinc-affinity chromatography. Furthermore the method may be used in either a column or batch format.
authors.affiliation: Department of Surgery (WGH), University of Edinburgh Medical School, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Cunningham
authors.forename: S F
authors.initials: SF
authors.lastname: Elliott
authors.forename: J R
authors.initials: JR
authors.lastname: Black
authors.forename: K
authors.initials: K
authors.lastname: James
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D016207:Cytokines / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D015203:Reproducibility of Results; D012685:Sepharose; D015032:Zinc; D000511:alpha-Macroglobulins / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D016207:Cytokines; D000511:alpha-Macroglobulins; D012685:Sepharose; D015032:Zinc