Antiplatelet granule membrane protein (GMP-140) autoantibodies detected in plasma from patients with idiopathic thrombocytopenic purpura.
Plasma from 92 patients with idiopathic thrombocytopenic purpura (ITP) was tested for autoantibodies against granule membrane protein 140 (GMP-140) using an antigen specific immunoassay. Results showed that autoantibodies to GMP-140 existed in 17 of these patients (18.5%). Incubation of patient plasma with purified GMP-140, immobilized on nitrocellulose strips by SDS-PAGE and Western blotting, revealed a staining band at the level of GMP-140 in two of 8 plasmas. Autoantibodies to GMP-140 often coexisted with other autoantibodies, in particular towards GP Ib/IX (3 cases), GP IIb-IIIa (5 cases) or both (3 cases). This study provides direct evidence for the presence of anti-platelet GMP-140 autoantibodies in some patients with ITP.
Jiang
H
H
Jiangsu Institute of Haematology, Suzhou Medical College, China.
Wu
G
G
Zhang
W
W
Zhao
Y
Y
Xi
X
X
Ruan
C
C
D000328:Adult; D001323:Autoantibodies / Q000097:blood*; D002648:Child; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D019007:P-Selectin; D010976:Platelet Count; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D016553:Purpura, Thrombocytopenic, Idiopathic / Q000097:blood / Q000209:etiology / Q000276:immunology*
D001323:Autoantibodies; D007074:Immunoglobulin G; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins