Diet effects and ontogeny of alterations of circulating insulin-like growth factor binding proteins in newborn dairy calves.
Insulin-like growth factors (IGF) are important growth regulators in many species, and their effects are influenced by their association with IGF-binding proteins (IGFBP). The objectives of this study were to characterize the ontogeny of the blood plasma IGFBP in calves and to determine the effect of dietary IGF-I neonatal plasma IGFBP. Plasma from newborn and 7-d-old male calves fed milk replacer, milk replacer + recombinant human IGF-I (rhIGF-I), or colostrum for 2 d followed by milk replacer was analyzed for IGFBP by ligand blot analysis. In addition, plasma samples from 1-, 12-, 24-, and 45-wk-old male calves were analyzed for IGFBP and IGF-I. Newborn and 7-d-old calf plasma contained IGFBP with M(r) of 26, 34, and 42 to 48 kDa. These profiles were not affected by the dietary treatments; however, a slight increase in the 34-kDa IGFBP and a slight decrease in the 26- and 42- to 48-kDa IGFBP were detected from birth to 7 d of age. The 34-kDa was confirmed to be bovine IGFBP-2 by immunoblot and the 42- to 48-kDa is likely IGFBP-3. The 29-, 31-, and 42- to 48-kDa IGFBP increased between 1 and 45 wk of age. Similarly, plasma IGF-I concentrations were increased from 49.7 to 449.7 ng/mL in plasma from calves from 1 to 45 wk of age. In contrast, the 34-kDa IGFBP increased from 1 to 12 wk but then gradually decreased from 12 to 45 wk, whereas the 26-kDa IGFBP did not change.(ABSTRACT TRUNCATED AT 250 WORDS)
Skaar
T C
TC
Department of Dairy and Animal Science, Pennsylvania State University, University Park 16802.
Baumrucker
C R
CR
Deaver
D R
DR
Blum
J W
JW
D000821:Animal Feed; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism*; D002352:Carrier Proteins / Q000097:blood*; D002417:Cattle / Q000254:growth & development / Q000378:metabolism*; D003126:Colostrum; D004032:Diet; D018969:Insulin-Like Growth Factor Binding Proteins; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D013002:Somatomedins / Q000378:metabolism*
D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D011994:Recombinant Proteins; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I