DNA variation in the genes of the Na,K-adenosine triphosphatase and its relation with resting metabolic rate, respiratory quotient, and body fat.
The aim of this study was to investigate in 261 subjects from 58 families the association between DNA variation at the genes coding for the Na,K-ATPase peptides and resting metabolic rate (RMR), respiratory quotient (RQ), and percent body fat (%FAT). Five restriction fragment length polymorphisms (RFLP) at three Na,K-ATPase genes were determined: one at the alpha 1 locus (BglII), and two at the beta locus (beta MspI and beta PvuII). Haplotypes were determined from the two variable sites of the alpha 2 gene (alpha 2 haplotypes) and the beta gene (beta haplotypes). There was a strong trend for %FAT to be related to the RFLP generated by BglII at the alpha 2 exons 21-22 in males (P = 0.06) and females (P = 0.05). RQ was (a) associated with the BglII RFLP at the alpha 2 exon 1 (P = 0.02) and with the alpha 2 8.0 kb/4.3 kb haplotype (P = 0.04) and (b) linked with the beta gene MspI marker (P = 0.04) and with the beta 5.3 kb/5.1 kb haplotype (P = 0.008) based on sib-pair analysis. The present study suggests that the genes encoding Na,K-ATPase may be associated or linked with RQ and perhaps with %FAT but not with RMR.
Dériaz
O
O
Physical Activity Sciences Laboratory, Laval University, Ste-Foy, Québec, Canada.
Dionne
F
F
Pérusse
L
L
Tremblay
A
A
Vohl
M C
MC
Côté
G
G
Bouchard
C
C
D000273:Adipose Tissue / Q000033:anatomy & histology*; D000328:Adult; D000367:Age Factors; D000483:Alleles; D000704:Analysis of Variance; D001426:Bacterial Proteins; D001481:Basal Metabolism; D015342:DNA Probes; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D005091:Exons; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D010101:Oxygen Consumption; D012150:Polymorphism, Restriction Fragment Length; D012737:Sex Factors; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*
D001426:Bacterial Proteins; D015342:DNA Probes; D019003:Deoxyribonuclease HpaII; C024404:BglII endonuclease; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D000254:Sodium-Potassium-Exchanging ATPase