title: Short consensus repeat-3 domain of recombinant decay-accelerating factor is recognized by Escherichia coli recombinant Dr adhesin in a model of a cell-cell interaction.
abstract: A bacterial pathogen that is important in both urinary tract and intestinal infections is Escherichia coli which expresses Dr or related adhesins. In this report, we present a model for testing cell-cell interaction, using both molecularly characterized laboratory cells that express recombinant molecules of human decay-accelerating factor (DAF), and recombinant bacterial Dr colonization factors. Dr adhesin ligand was identified as DAF (CD55), a membrane protein that protects autologous tissues from damage due to the complement system. Structure-function studies mapped the adhesin-binding site on the DAF molecule. A single-point substitution in the third short consensus repeat domain, Ser165 to Leu, corresponding to the Dra to Drb allelic polymorphism, caused complete abolition of adhesin binding to DAF.
authors.affiliation: Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston 77550.
authors.forename: B
authors.initials: B
authors.lastname: Nowicki
authors.forename: A
authors.initials: A
authors.lastname: Hart
authors.forename: K E
authors.initials: KE
authors.lastname: Coyne
authors.forename: D M
authors.initials: DM
authors.lastname: Lublin
authors.forename: S
authors.initials: S
authors.lastname: Nowicki
mesh_terms: D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial / Q000378:metabolism*; D015703:Antigens, CD / Q000737:chemistry / Q000378:metabolism*; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001665:Binding Sites; D018958:CD55 Antigens; D002448:Cell Adhesion; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins; D013329:Structure-Activity Relationship
chemical_list: D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial; D015703:Antigens, CD; D001425:Bacterial Outer Membrane Proteins; D018958:CD55 Antigens; D008024:Ligands; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins