title: Expression of c-kit ligand in human keratinocytes.
abstract: The c-kit ligand is expressed on tissue-anchored stromal cells. It plays an important role in the development of c-kit-bearing cells, such as haematopoietic cells, germ cells, mast cells and melanocytes. In the present study, we used the reverse transcriptase-mediated polymerase chain reaction (PCR) technique to investigate whether human keratinocytes are able to express c-kit ligand mRNA. Two sets of primers were designed to distinguish two types of c-kit ligand mRNA (full-length type and spliced type). One set was used to amplify an 882-bp DNA fragment from the full-length type, and a 798-bp DNA fragment from the spliced type. Another set was used to amplify a 375-bp DNA fragment from the full-length type only. A cDNA fragment corresponding to the full-length type mRNA was amplified from a cDNA preparation of cultured human keratinocytes as well as from epidermis obtained by the suction blister technique. This result indicates the spontaneous transcription of full-length type mRNA of the c-kit ligand in human keratinocytes.
authors.affiliation: Department of Dermatology, Hiroshima University School of Medicine, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Morita
authors.forename: D G
authors.initials: DG
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Sugiyama
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D004817:Epidermis / Q000378:metabolism; D016298:Hematopoietic Cell Growth Factors / Q000096:biosynthesis*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D019089:Stem Cell Factor
chemical_list: D016298:Hematopoietic Cell Growth Factors; D012333:RNA, Messenger; D019089:Stem Cell Factor