title: Detection of Pseudomonas pseudomallei antigen in urine for the diagnosis of melioidosis.
abstract: An enzyme-linked immunosorbent assay using a fluorescein isothiocyanate (FITC)-anti-FITC amplification system, has been developed to detect Pseudomonas pseudomallei antigen in urine. The assay was evaluated in 135 patients with acute melioidosis, 194 hospitalized patients with other disorders, and 40 healthy controls. Antigen was detected in the urine of 123 (91%) patients with melioidosis. Urinary antigen was found in 85 (96%) of 89 patients with septicemic melioidosis, all six patients with P. pseudomallei urinary tract infection, and 32 (80%) of 40 patients with other localized infections. Antigen was not detected in the urine of 40 healthy individuals, but the urine of 16 (8%) of 194 hospitalized patients with diagnoses other than melioidosis gave a positive result. Of the false-positive results, 13 of 16 were associated with bacteriuria > or = 10(4) colony-forming units/ml. At a cutoff titer of 1:10, the sensitivity and specificity of the test were 81% and 96%, respectively. Enzyme immunoassay detection of urinary antigen is a valuable and rapid laboratory test for the early diagnosis of acute melioidosis.
authors.affiliation: Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: V
authors.initials: V
authors.lastname: Desakorn
authors.forename: M D
authors.initials: MD
authors.lastname: Smith
authors.forename: V
authors.initials: V
authors.lastname: Wuthiekanun
authors.forename: D A
authors.initials: DA
authors.lastname: Dance
authors.forename: H
authors.initials: H
authors.lastname: Aucken
authors.forename: P
authors.initials: P
authors.lastname: Suntharasamai
authors.forename: A
authors.initials: A
authors.lastname: Rajchanuwong
authors.forename: N J
authors.initials: NJ
authors.lastname: White
mesh_terms: D000208:Acute Disease; D000818:Animals; D000942:Antigens, Bacterial / Q000652:urine*; D016470:Bacteremia / Q000175:diagnosis; D001437:Bacteriuria / Q000175:diagnosis; D016957:Burkholderia pseudomallei / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D008554:Melioidosis / Q000175:diagnosis* / Q000453:epidemiology; D011446:Prospective Studies; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013785:Thailand / Q000453:epidemiology; D014552:Urinary Tract Infections / Q000175:diagnosis
chemical_list: D000942:Antigens, Bacterial; D016650:Fluorescein-5-isothiocyanate