A behaviorally active dose of lipopolysaccharide increases sensory neuropeptides levels in mouse spinal cord.
To assess whether peripheral immune stimuli activate sensory afferents at behaviorally active doses, we measured the effects of lipopolysaccharide (LPS) on the levels of sensory neuropeptides in the spinal cord. LPS (10 micrograms/mouse i.p.) increased the levels of substance P, neurokinin A, and calcitonin gene-related peptide in the spinal cord, the maximum being observed 1 hr post-injection. Pretreatment with indomethacin at a dose (5 mg/kg i.p.) which completely blocked the decrease in food-motivated behavior induced by LPS abrogated this effect.
Bret-Dibat
J L
JL
INSERM U394, Bordeaux, France.
Kent
S
S
Couraud
J Y
JY
Creminon
C
C
Dantzer
R
R
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D015740:Calcitonin Gene-Related Peptide / Q000276:immunology / Q000378:metabolism; D005247:Feeding Behavior / Q000187:drug effects; D005502:Food; D007118:Immunoassay; D007213:Indomethacin / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008297:Male; D051379:Mice; D015288:Neurokinin A / Q000276:immunology / Q000378:metabolism; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013373:Substance P / Q000276:immunology / Q000378:metabolism
D008070:Lipopolysaccharides; D009479:Neuropeptides; D013373:Substance P; D015288:Neurokinin A; D015740:Calcitonin Gene-Related Peptide; D007213:Indomethacin