Okadaic acid blocks PDGF-induced proliferation of AKR-2B fibroblasts at the transition from G1- to S-phase.
Okadaic acid (OA) at 100 ng/ml completely inhibited platelet-derived growth factor (PDGF)-BB-induced DNA synthesis but had no effect on early signals, i.e., PDGF receptor autophosphorylation or stimulation of inositolphosphate turnover. A detailed analysis using synchronized cells showed that OA acts at the transition from G1-phase to S-phase. These observations were confirmed by flow cytometric DNA analysis of asynchronously grown cells. Here cells were specifically arrested in the G1-phase.
Simm
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Theodor-Boveri-Institut für Biowissenschaften (Biozentrum) der Universität, Physiologische Chemie II, Würzburg, Germany.
Karenberg
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TA
Hoppe
V
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Hoppe
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J
D000818:Animals; D000077214:Becaplermin; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004988:Ethers, Cyclic / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016193:G1 Phase / Q000187:drug effects; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008806:Mice, Inbred AKR; D019319:Okadaic Acid; D019000:Phosphotyrosine; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors*; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins / Q000494:pharmacology; D016196:S Phase / Q000187:drug effects; D015398:Signal Transduction / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism
D004988:Ethers, Cyclic; D007295:Inositol Phosphates; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D000077214:Becaplermin; D019319:Okadaic Acid; D019000:Phosphotyrosine; D014443:Tyrosine; D004247:DNA; D017027:Protein Tyrosine Phosphatases; D013936:Thymidine