Selective expression of the glutamate receptor channel delta 2 subunit in cerebellar Purkinje cells.
The primary structure of a putative subunit of the mouse glutamate receptor channel, designated as the delta 2 subunit, has been deduced by cloning and sequencing the cDNA. The delta 2 subunit has four putative transmembrane segments characteristic for neurotransmitter-gated ion channels, and shares 56% amino acid sequence identity with the delta 1 subunit of the mouse glutamate receptor channel and 14-24% identity with the subunits of the AMPA-, kainate- or NMDA-selective glutamate receptor channel. RNA blot and in situ hybridization analyses show that the delta 2 subunit mRNA is localized in cerebellar Purkinje cells. Furthermore, immunoblot and immunohistochemical analyses suggest that the delta 2 subunit protein is actually expressed in vivo in Purkinje neurons. The selective localization of the delta 2 subunit in Purkinje cells may imply a role of the delta 2 subunit in Purkinje cell-specific function such as the cerebellar LTD.
Araki
K
K
Department of Neuropharmacology, Niigata University, Japan.
Meguro
H
H
Kushiya
E
E
Takayama
C
C
Inoue
Y
Y
Mishina
M
M
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002462:Cell Membrane / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D015151:Immunoblotting; D017403:In Situ Hybridization; D007473:Ion Channels / Q000096:biosynthesis* / Q000302:isolation & purification; D046911:Macromolecular Substances; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016548:Prosencephalon / Q000378:metabolism*; D011689:Purkinje Cells / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D017470:Receptors, Glutamate / Q000096:biosynthesis* / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D007473:Ion Channels; D046911:Macromolecular Substances; D012333:RNA, Messenger; D017470:Receptors, Glutamate