Morphology, growth, and gene expression in five newly isolated murine hepatocellular tumor cell lines.
Five murine hepatocellular tumor cell lines (HepM-1-5) were isolated and grown in a synthetic medium added with hormones, growth factors and/or serum. The morphology of these lines ranged from a nearly homogeneous epithelial-like shape (HepM-2) to a stromal appearance (HepM-1). The remaining lines displayed a mixed morphology. For their proliferation all of the cell lines retained a clear dependence on the extracellular calcium level and hormonal and/or serum growth factors and, rather homogeneously, they did not express the albumin, alpha-fetoprotein (with the exception of HepM-2 cells), tyrosine aminotransferase, and ornithine transcarbamylase genes, whereas they all exhibited discrete levels of the ornithine aminotransferase mRNA. Only HepM-3 and HepM-5 lines expressed the procollagen type I gene.
Dragani
T A
TA
Division of Experimental Oncology A, Istituto Nazionale Tumori, Milan, Italy.
Ribecco
M
M
Manenti
G
G
Pierotti
M A
MA
Testolin
L
L
Guerriero
C
C
Menapace
L
L
Armato
U
U
D000818:Animals; D002455:Cell Division; D003094:Collagen / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D066298:In Vitro Techniques; D008099:Liver / Q000502:physiology; D008114:Liver Neoplasms, Experimental / Q000503:physiopathology*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D009954:Ornithine Carbamoyltransferase / Q000235:genetics; D009953:Ornithine-Oxo-Acid Transaminase / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics*; D012709:Serum Albumin / Q000235:genetics; D014407:Tumor Cells, Cultured; D014444:Tyrosine Transaminase / Q000235:genetics; D000509:alpha-Fetoproteins / Q000235:genetics
D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012709:Serum Albumin; D000509:alpha-Fetoproteins; D003094:Collagen; D009954:Ornithine Carbamoyltransferase; D009953:Ornithine-Oxo-Acid Transaminase; D014444:Tyrosine Transaminase