In vitro primary responses of human T cells to soluble protein antigens.
The optimum culture conditions to support in vitro proliferative responses to conventional soluble protein antigens by human CD4+ T cells from healthy non-immunised donors have been determined. These responses were blocked by anti-HLA-DR monoclonal antibodies (mAbs) and were strictly dependent on the presence of antigen-presenting cells (APCs). Primary responses showed a reproducible pattern of proliferation kinetics which distinguished them from secondary in vitro T cells reactions. T cells specific for the sensitising antigen were recovered from primary cultures.
Plebanski
M
M
Department of Pathology and Microbiology, Medical School, University of Bristol, UK.
Burtles
S S
SS
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D000938:Antigen-Presenting Cells / Q000276:immunology; D000941:Antigens / Q000276:immunology; D002478:Cells, Cultured; D006684:HLA-DR Antigens / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D017493:Leukocyte Common Antigens / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D015203:Reproducibility of Results; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology
D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D000941:Antigens; D006684:HLA-DR Antigens; D014373:Tuberculin; D005719:gamma-Globulins; D006433:Hemocyanins; D017493:Leukocyte Common Antigens; C032808:keyhole-limpet hemocyanin