alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionate and kainate differently affect neuronal cytoarchitecture of rat cerebellar granule cells.
Rat cerebellar granule cells cultured in media containing 12 mM KCl showed short life-span, did not branch, and died after 10 days in vitro. The cell exposure to N-methyl-D-aspartate (NMDA) or to kainate promoted both neuron survival and branching, reproducing the viability and the neurite extension routinely observed in cultures maintained in media containing 25 mM KCl. Exposure of neurons to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) resulted in an increased survival not associated with neuritic arborization. These results suggest that the glutamate ionotropic receptor subtypes differently contribute in elaborating neuronal morphogenesis.
Pizzi
M
M
Department of Biomedical Science and Biotechnologies, School of Medicine, University of Brescia, Italy.
Fallacara
C
C
Consolandi
O
O
Memo
M
M
Spano
P F
PF
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology* / Q000187:drug effects; D007608:Kainic Acid / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017459:Receptors, Amino Acid / Q000187:drug effects; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology*
D017459:Receptors, Amino Acid; D011189:Potassium Chloride; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D007608:Kainic Acid