Recruitment of pathogenic T cells to synovial tissues of rats injected intraarticularly with nonspecific agents.
To study a role for T cells in nonspecifically induced arthritis, rats were injected intraarticularly into ankle joints with mineral oils including 2,6,10,15,19,23-hexamethyltetracosane (squalene) and incomplete Freund's adjuvant. The results showed that moderate joint inflammation had developed by Day 6 after the nonspecific stimulation. This was then followed by induction of severe arthritis, reaching a peak on Day 21. Histologically, the early phase of arthritis was associated with edema of synovial tissues containing many polymorphs and monocytes/macrophages and the late phase of the joint inflammation with marked hyperplasia of synovial membrane with dense infiltration of CD5+ and alpha beta+ T cells. Depletion of alpha beta+ T cells by a monoclonal antibody against TCR alpha beta suppressed both induction and progression of the late phase of arthritis. Thus, pathogenic T cells appear to be recruited to joint tissues following nonspecific stimulation.
Yoshino
S
S
Rheumatology Unit, Royal Adelaide Hospital, Australia.
Yoshino
J
J
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D015703:Antigens, CD / Q000276:immunology; D001169:Arthritis, Experimental / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D018956:CD5 Antigens; D003094:Collagen / Q000276:immunology; D005260:Female; D007124:Immunoenzyme Techniques; D007270:Injections, Intra-Articular; D051381:Rats; D011919:Rats, Inbred Strains; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013185:Squalene / Q000276:immunology; D013583:Synovial Membrane / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018956:CD5 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013185:Squalene; D003094:Collagen