Monitoring of treatment outcome in acute promyelocytic leukemia by RT-PCR.
A rearrangement between the PML and RAR-alpha genes underlies the acute promyelocytic leukemia (APL)-specific t(15;17) translocation, leading to the production of a chimeric mRNA. Recent development of a reverse-transcription polymerase chain reaction (RT-PCR) assay for the PML/RAR-alpha hybrid has proven useful for rapid diagnosis and monitoring of minimal residual disease (MRD) in APL patients. Preliminary studies in which the prognostic significance of RT-PCR was evaluated indicate that this test may identify patients at high risk of relapse.
Diverio
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D
Department of Human Biopathology, University La Sapienza, Rome, Italy.
Pandolfi
P P
PP
Rossi
V
V
Biondi
A
A
Pelicci
P G
PG
Lo Coco
F
F
D006402:Hematologic Diseases / Q000235:genetics / Q000628:therapy; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000628:therapy; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000628:therapy*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011379:Prognosis; D012194:RNA-Directed DNA Polymerase; D016896:Treatment Outcome
D012194:RNA-Directed DNA Polymerase