A transient increase of phenylalanine ammonia-lyase transcript in kinetin-treated tobacco callus.
In tobacco cell culture (Nicotiana tabacum L. "Bright Yellow" T-13), phenylalanine ammonia-lyase (PAL) activity was induced in response to an exogenously added kinetin. RNA blot hybridization analysis showed that a single species of PAL transcript 2.9-kb in size was detected using the PAL cDNA cloned from kinetin-treated cells. The cellular content of this transcript increased transiently (2.5-fold) 4 h after the addition of kinetin followed by an increase in PAL enzyme activity at 16 h.
Nagai
N
N
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Kitauchi
F
F
Okamoto
K
K
Kanda
T
T
Shimosaka
M
M
Okazaki
M
M
D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D017403:In Situ Hybridization; D007701:Kinetin; D008969:Molecular Sequence Data; D010650:Phenylalanine Ammonia-Lyase / Q000096:biosynthesis / Q000235:genetics*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010947:Plants, Toxic; D012313:RNA / Q000032:analysis; D014026:Nicotiana / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*
D010937:Plant Growth Regulators; D012313:RNA; D010650:Phenylalanine Ammonia-Lyase; D000225:Adenine; D007701:Kinetin