The suppressor factor of T suppressor cells induced by tolerogenic conjugates of ovalbumin and monomethoxypolyethylene glycol is serologically and physicochemically related to the alpha beta heterodimer of the T cell receptor.
Ovalbumin-specific, H-2Kd restricted, CD8+ Ts cells of clone 17.2 were shown to produce an OVA-specific Ts cell factor (TsF17.2) possessing the same Ag specificity and MHC restriction as those of the intact Ts cells. The Ts cell clone was generated from a single cell of the spleen of a mouse which had been immunosuppressed by injection of tolerogenic OVA(mPEG)12 conjugate. For the elucidation of the nature of TsF17.2, it was characterized by serologic, physicochemical, and Western blot analyses. It was found that 1) the OVA-specific suppression of in vitro antibody production by TsF17.2 could be blocked by mAb H28-710 which binds to an epitope of the constant region of the alpha-chain of TCR; 2) the TsF17.2 could be sequestered by, and eluted from, immunosorbents prepared by coupling to Affi-Gel Hz the H28-710 mAb or the mAb H57-597 and F23.1 which are specific, respectively, for an epitope of the constant region of the beta-chain and an epitope of the V beta 8 region of the TCR; and 3) the TsF17.2 had a pl of 7.0, m.w. of 84,000, and consisted of two disulfide-linked subunits of 42,000 each. After electroelution from the SDS-PAGE gel, the m.w. 84,000 molecule retained its capacity to suppress in vitro antibody production in an OVA-specific manner. From all these results it was concluded that this Ts cell factor may represent a soluble form of the alpha beta heterodimer of TCR of cloned Ts cells.
Chen
Y
Y
Department of Immunology, University of Manitoba, Winnipeg, Canada.
Takata
M
M
Maiti
P K
PK
Mohapatra
S
S
Mohapatra
S S
SS
Sehon
A H
AH
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002999:Clone Cells; D000939:Epitopes / Q000032:analysis; D005260:Female; D006183:H-2 Antigens; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D010047:Ovalbumin / Q000276:immunology; D011092:Polyethylene Glycols; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry* / Q000276:immunology; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D000939:Epitopes; D006183:H-2 Antigens; C024755:H-2K(K) antigen; D016693:Receptors, Antigen, T-Cell, alpha-beta; D013491:Suppressor Factors, Immunologic; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D010047:Ovalbumin