Potential, pH, and arachidonate gate hydrogen ion currents in human neutrophils.
Indirect evidence indicates that a proton-selective conductance is activated during the respiratory burst in neutrophils. A voltage- and time-dependent H(+)-selective conductance, gH, in human neutrophils is demonstrated here directly by the whole-cell patch-clamp technique. The gH is extremely low at large negative potentials, increases slowly upon membrane depolarization, and does not inactivate. It is enhanced at high external pH or low internal pH and is inhibited by Cd2+ and Zn2+. Arachidonic acid, which plays a pivotal role in inflammatory reactions, amplifies the gH. The properties of the gH described here are compatible with its activation during the respiratory burst in stimulated neutrophils, in which it may facilitate sustained superoxide anion release by dissipating metabolically generated acid.
DeCoursey
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TE
Department of Physiology, Rush Presbyterian St. Luke's Medical Center, Chicago, Illinois 60612.
Cherny
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VV
D016718:Arachidonic Acid / Q000494:pharmacology; D055592:Biophysical Phenomena; D001703:Biophysics; D002104:Cadmium / Q000494:pharmacology; D004553:Electric Conductivity; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000187:drug effects; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D011522:Protons; D016897:Respiratory Burst; D015032:Zinc / Q000494:pharmacology
D007473:Ion Channels; D011522:Protons; D002104:Cadmium; D016718:Arachidonic Acid; D015032:Zinc