Fas-based lymphocyte-mediated cytotoxicity against syngeneic activated lymphocytes: a regulatory pathway?
To investigate the possibility that Fas-based immune regulation and Fas-based T cell-mediated cytotoxicity (F-CMC) are causally related, we explored the latter in activated lymphocyte populations. These were shown to contain effector cells exerting cytotoxicity via an F-CMC mechanism which could be differentially triggered by PMA and ionomycin. F-CMC operated in trans, requiring an lpr (Fas) product on target cells and a gld product on effector cells. Activated lymphocyte populations were also shown to contain F-CMC target cells. Activated lymphocyte populations thus contained both effector and target F-CMC cells, which could lyse each other. Fas-based cytotoxicity can thus lead to the lysis of syngeneic activated lymphocytes, consistent with the possibility of its participation in the down-regulation of immune responses, and more generally offering a model of socially controlled, direct membrane-mediated cell death.
Vignaux
F
F
Centre d'Immunologie, INSERM-CNRS, Marseille-Luminy, France.
Golstein
P
P
D000818:Animals; D000954:Antigens, Surface / Q000502:physiology*; D016923:Cell Death; D003602:Cytotoxicity, Immunologic; D015759:Ionomycin / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D013601:T-Lymphocytes / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D019014:fas Receptor
D000954:Antigens, Surface; D019014:fas Receptor; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate