The role of calcium and alpha-adrenoceptors in contractile response of chick expansor secundariorum muscle to field stimulation.
1. The effects of some alpha-adrenergic agonists and antagonists on electrically-evoked contractions and tension of chick expansor secundariorum muscle (ESM), and dependence of these events on extracellular calcium was investigated. 2. Both train and continuous electrical stimulation can produce regular contractions in preparations obtained from 40-60 day old chicks. 3. Clonidine had a biphasic action on the contractions produced by train electrical stimulation. In concentrations ranging from 10(-8) to 3 x 10(-7) M, clonidine decreased the contraction amplitude, but in higher concentrations, it caused an increase in both the muscle tension and the contraction amplitude. These effects were reversed by application of yohimbine although yohimbine by itself had no effect on the contractions. 4. Introduction of calcium free isotonic high potassium medium decreased muscle tone which was followed by further dose-dependent increase in tension, along with the addition of cumulative doses of CaCl2 (ED50 = 2.8 x 10(-3) M). 5. Nifedipine reduced the amplitude of ESM contractions produced by continuous electrical stimulation in a dose dependent manner (IC50 = 6.7 x 10(-7) M). 6. Methoxamine induced a completely dose dependent increase in muscle tension which was dependent on extracellular calcium and was inhibited by nifedipine. In the presence of 10(-8) M nifedipine, ED50 of methoxamine stimulatory effect increased from the control value of 2.2 x 10(-7) to 8.4 x 10(-7) M).(ABSTRACT TRUNCATED AT 250 WORDS)
Dehpour
A R
AR
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Iran.
Tajkhorshid
E
E
Behbahani
N R
NR
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology*; D002122:Calcium Chloride / Q000494:pharmacology; D002645:Chickens; D003000:Clonidine / Q000494:pharmacology; D004558:Electric Stimulation; D000431:Ethanol / Q000494:pharmacology; D066298:In Vitro Techniques; D008729:Methoxamine / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects; D009132:Muscles / Q000294:innervation / Q000502:physiology*; D009543:Nifedipine / Q000494:pharmacology; D011942:Receptors, Adrenergic, alpha / Q000187:drug effects / Q000502:physiology*; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology; D015016:Yohimbine / Q000494:pharmacology
D011942:Receptors, Adrenergic, alpha; D015016:Yohimbine; D000431:Ethanol; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D008729:Methoxamine; D009543:Nifedipine; D002122:Calcium Chloride; D003000:Clonidine; D002118:Calcium