title: Expression of the estrogen receptor in human thyroid neoplasms.
abstract: The expression and quantitation of the estrogen receptor (ER) in human thyroid tumors were examined by biochemical, immunohistochemical, and reverse transcriptase-polymerase chain reaction (RT-PCR) techniques. For this study, neoplasms, adenomatous goiters and adjacent normal thyroid tissues were obtained from 35 patients which included 10 cases of papillary carcinomas, 17 cases of adenomas and 8 cases of adenomatous goiters. Regardless of the histopathological subtype, ER was detected in 19% (5/27) of the neoplastic tissues with the mean value of ER content of 5.0 +/- 1.3 fmol/mg protein and the mean Kd value of 0.38 +/- 0.28 nM. ER was also detected, but at a lower concentration (2.8 +/- 1.6 fmol/mg protein), in the surrounding normal tissues. There was no significant difference between the neoplasms and adenomatous goiters with respect to the incidence of ER positivity and ER content. Furthermore, ER-positive specimens, as determined by both biochemical and immunohistochemical techniques, also showed the expression of ER mRNA detected by RT-PCR method. These results demonstrate that both ER mRNA as well as ER protein are expressed in thyroid neoplasms. This suggests the possibility that estrogen may affect the tumorigenesis or the progression of some thyroid neoplasms.
authors.affiliation: Department of Otorhinolaryngology, Nara Medical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yane
authors.forename: Y
authors.initials: Y
authors.lastname: Kitahori
authors.forename: N
authors.initials: N
authors.lastname: Konishi
authors.forename: K
authors.initials: K
authors.lastname: Okaichi
authors.forename: T
authors.initials: T
authors.lastname: Ohnishi
authors.forename: H
authors.initials: H
authors.lastname: Miyahara
authors.forename: T
authors.initials: T
authors.lastname: Matsunaga
authors.forename: J C
authors.initials: JC
authors.lastname: Lin
authors.forename: Y
authors.initials: Y
authors.lastname: Hiasa
mesh_terms: D000236:Adenoma / Q000737:chemistry / Q000648:ultrastructure; D000328:Adult; D000368:Aged; D001483:Base Sequence; D002291:Carcinoma, Papillary / Q000737:chemistry / Q000648:ultrastructure; D002606:Charcoal; D003911:Dextrans; D005260:Female; D006042:Goiter / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis; D012194:RNA-Directed DNA Polymerase; D011960:Receptors, Estrogen / Q000032:analysis*; D013964:Thyroid Neoplasms / Q000737:chemistry / Q000648:ultrastructure*
chemical_list: D003911:Dextrans; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D002606:Charcoal; D012194:RNA-Directed DNA Polymerase