title: [Derivatives of ddUTP, modified at the 5-position of uridine, as substrate terminators of reverse transcriptase. Hydrolysis of oligonucleotides, terminated by these analogs, by phosphodiesterase I].
abstract: Synthesis of 2',3'-dideoxyuridine 5'-triphosphate analogues with fluorescent and biotin residues at C5 of uracil base was carried out. The substrate properties of these analogues were studied with AMV, M-MLV, and HIV reverse transcriptase. All 5-derivatives studied were shown to be incorporated into the 3'-terminus of oligonucleotide. The stability of oligodeoxyribonucleotides terminated with ddUTP analogues modified at the 5-position of the pyrimidine residue to the exonuclease action of phosphodiesterase I and Klenow enzyme was more than 1000 times higher than that of nonterminated oligonucleotides.
authors.forename: L B
authors.initials: LB
authors.lastname: D'iachenko
authors.forename: A A
authors.initials: AA
authors.lastname: Chenchik
authors.forename: M A
authors.initials: MA
authors.lastname: Lukin
authors.forename: L A
authors.initials: LA
authors.lastname: Aleksandrova
authors.forename: A A
authors.initials: AA
authors.lastname: Kraevskiĭ
authors.forename: R Sh
authors.initials: RSh
authors.lastname: Bibilashvili
mesh_terms: D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001483:Base Sequence; D004256:DNA Polymerase I / Q000378:metabolism; D054306:Dideoxynucleotides; D006678:HIV / Q000201:enzymology; D006868:Hydrolysis; D008324:Mammary Tumor Virus, Mouse / Q000201:enzymology; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D013379:Substrate Specificity; D013728:Terminator Regions, Genetic; D014500:Uracil Nucleotides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D014529:Uridine / Q000737:chemistry*
chemical_list: D054306:Dideoxynucleotides; D009838:Oligodeoxyribonucleotides; D014500:Uracil Nucleotides; C063034:2',3'-dideoxyuridine-5'-triphosphate; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; D014529:Uridine