title: Novel endothelial cell activation factor(s) released from activated platelets which induce E-selectin expression and tumor cell adhesion to endothelial cells: a preliminary note.
abstract: Activation of endothelial cells (ECs) by tumor cells (TCs) to elicit expression of adhesion receptors such as selectins and intercellular adhesion molecules (ICAMs) has been considered to facilitate TC-EC adhesion, the initial step in TC metastasis. Studies reported here indicate that: (i) TC lines tested did not activate ECs; (ii) tested TCs activated native (but not inert) platelets, which in turn activated ECs to express E-selectin, leading to TC-EC adhesion; (iii) auto-aggregation of TCs eventually resulted in adhesion of large TC aggregates to ECs; (iv) process ii is inhibited by H7 and calphostin, indicating involvement of a signaling process mediated by protein kinases in expression of E-selectin.
authors.affiliation: Biomembrane Institute, Seattle, WA 98119.
authors.forename: S
authors.initials: S
authors.lastname: Hakomori
mesh_terms: D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D000470:Alkaloids / Q000494:pharmacology; D000818:Animals; D001685:Biological Factors / Q000097:blood* / Q000502:physiology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002450:Cell Communication; D002478:Cells, Cultured; D003110:Colonic Neoplasms; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000502:physiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000502:physiology; D007546:Isoquinolines / Q000494:pharmacology; D007700:Kinetics; D008175:Lung Neoplasms; D008546:Melanoma, Experimental; D051379:Mice; D008954:Models, Biological; D009281:Naphthalenes; D009369:Neoplasms / Q000503:physiopathology*; D010879:Piperazines / Q000494:pharmacology; D015539:Platelet Activation; D010974:Platelet Aggregation; D011083:Polycyclic Compounds / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000502:physiology; D019311:Staurosporine; D013274:Stomach Neoplasms; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins
chemical_list: D000470:Alkaloids; D001685:Biological Factors; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D007546:Isoquinolines; D009281:Naphthalenes; D010879:Piperazines; D011083:Polycyclic Compounds; D047428:Protein Kinase Inhibitors; C061465:calphostin complex; D018799:Intercellular Adhesion Molecule-1; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D011494:Protein Kinases; D019311:Staurosporine