Neuritogenic Lewis rat T cells use Tcrb chains that include a new Tcrb-V8 family member.
The P2 protein obtained from Schwann cells induces a population of T cells which, upon adoptive transfer, causes the disease experimental allergic neuritis (EAN), an animal model for Guillain-Barre syndrome. In this report, a truncated peptide, FR22, derived from a previously reported neuritogenic T-cell determinant, was used to generate from Lewis rats T cells that were shown to cause EAN. Since our previous studies showed that Tcrb-V8 was used by a majority of T-cell hybridomas specific for the neuritogenic peptide P26, which contains the FR22 sequence, we sequenced the Tcrb-V8+ mRNA from FR22-specific T-cell lines, and compared the sequences obtained with those obtained from similarly generated myelin basic protein (MBP) 68-88-specific Lewis rat T-cell lines. We found that in the EAN lines, several members of the Tcrb-V8 family were used, including a new family member, Tcrb-V8E. This was more diverse than the MBP-68-88-specific response in which only a single Tcrb-V8 family member was used. Also, in the EAN lines, the beta chain sequences did not show the same conserved junctional regions seen in the MBP lines. Thus, T-cell receptor beta chain usage in the response to this dominant neuritogenic peptide appears to be less restricted than the response to the dominant encephalitogenic determinant of MBP both in V region usage and in CDR3 usage.
Zhang
X M
XM
Wistar Institute, Philadelphia, PA 19104.
Esch
T R
TR
Clark
L
L
Gregorian
S
S
Rostami
A
A
Otvos
L
L
Heber-Katz
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D005796:Genes; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000276:immunology*; D018996:Myelin P2 Protein; D016501:Neurites; D009444:Neuritis, Autoimmune, Experimental / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D004676:Myelin Basic Protein; D018996:Myelin P2 Protein; D010446:Peptide Fragments; D010455:Peptides; D016693:Receptors, Antigen, T-Cell, alpha-beta; C076290:myelin P2 peptide SP26