title: Paranuclear blue inclusions: an aid in the cytopathologic diagnosis of primary and metastatic pulmonary small-cell carcinoma.
abstract: Accurate diagnosis of small-cell carcinoma of the lung (SCLC) is clinically important because of the therapeutic implications. SCLC must be distinguished from non-small-cell carcinoma (NSCLC) and lymphoma. Paranuclear blue inclusions (PBIs) were recently described as a feature of metastatic SCLC on air-dried Wright-stained bone marrow aspirate smears. To determine the utility of PBIs in distinguishing SCLC from NSCLC and lymphoma, we evaluated air-dried Diff-Quik-stained smears from 103 fine-needle aspiration (FNA) specimens and 14 touch imprint specimens. PBIs were identified in 24 (89%) of 27 cases of SCLC, in 6 (9%) of 64 non-small-cell carcinomas (P < 0.00001), and in two (8%) of the 26 lymphoma cases (P < 0.00001). No PBIs were seen on any of the alcohol-fixed Papanicolaou or hematoxylin-eosin (H&E) stained smears examined. In conclusion, PBIs appear to be a feature of SCLC on air-dried cytologic material stained with Romanowsky type stains. In the presence of cytologic features of SCLC, the identification of PBIs provides a useful diagnostic feature for differentiating between SCLC and NSCLC carcinomas, and between SCLC and lymphomas in FNA specimens and touch imprints from surgical specimens.
authors.affiliation: Pathology and Laboratory Medicine Service, Veterans Affairs Medical Center, Johnson City, TN.
authors.forename: R K
authors.initials: RK
authors.lastname: Mullins
authors.forename: S K
authors.initials: SK
authors.lastname: Thompson
authors.forename: P S
authors.initials: PS
authors.lastname: Coogan
authors.forename: M S
authors.initials: MS
authors.lastname: Shurbaji
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000473:pathology; D018288:Carcinoma, Small Cell / Q000473:pathology* / Q000556:secondary; D003581:Cytodiagnosis / Q000379:methods; D003937:Diagnosis, Differential; D006801:Humans; D002479:Inclusion Bodies / Q000473:pathology*; D008175:Lung Neoplasms / Q000473:pathology*; D008223:Lymphoma / Q000473:pathology; D012189:Retrospective Studies; D013194:Staining and Labeling