Differentiation of Pseudomonas aeruginosa pili based on sequence and B-cell epitope analyses.
The nucleotide sequences of three previously undescribed Pseudomonas aeruginosa pilin structural genes are presented. Comparisons of deduced pilin primary structure and flanking DNA sequence allowed placement of these and six previously published sequences into one of two groups. Epitope mapping, using overlapping immobilized peptides representing the pilin primary structure, with antipilin monoclonal antibodies revealed several B-cell determinants grouped near the carboxyl terminus of P. aeruginosa 1244 pilin. One determinant was found to reside near the pilin constant region. These determinants were found associated with the pili of 31 of 95 P. aeruginosa clinical isolates.
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Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282.
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D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000235:genetics*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D000939:Epitopes / Q000235:genetics; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000276:immunology*; D005798:Genes, Bacterial; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; D000939:Epitopes; D036781:Fimbriae Proteins