Control of cell behavior during vertebrate development by Slug, a zinc finger gene.
Slug, a vertebrate gene encoding a zinc finger protein of the Snail family, is expressed in the neural crest and in mesodermal cells emigrating from the primitive streak. Early chick embryos were incubated with antisense oligonucleotides to chick Slug. These oligonucleotides specifically inhibit the normal change in cell behavior that occurs at the two sites in the emerging body plan in which the gene is expressed. This change, which is the transition from epithelial to mesenchymal character, occurs at the formation of mesoderm during gastrulation and on emigration of the neutral crest from the neural tube.
Nieto
M A
MA
Instituto Cajal, Madrid, Spain.
Sargent
M G
MG
Wilkinson
D G
DG
Cooke
J
J
D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001483:Base Sequence; D001756:Blastoderm / Q000166:cytology; D018959:CD57 Antigens; D002465:Cell Movement; D002490:Central Nervous System / Q000196:embryology*; D002642:Chick Embryo; D005775:Gastrula / Q000166:cytology*; D015870:Gene Expression; D017403:In Situ Hybridization; D008648:Mesoderm / Q000166:cytology*; D008969:Molecular Sequence Data; D009432:Neural Crest / Q000166:cytology / Q000276:immunology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D014158:Transcription, Genetic; D016335:Zinc Fingers / Q000235:genetics*
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018959:CD57 Antigens; D016376:Oligonucleotides, Antisense; D000071250:Snail Family Transcription Factors; D014157:Transcription Factors