Epitope discovery using peptide libraries displayed on phage.
Peptides displayed on phage, which mimic continuous and discontinuous epitopes, can be selected using purified antibodies or preparations of polyclonal serum. This review describes recent advances in this field, discusses the application of phage-display technology to the diagnosis of human diseases, and presents new ideas for the preparation of vaccines directed against specific epitopes on a pathogen.
Cortese
R
R
Istituto di Ricerche di Biologia Molecolare P. Angeletti, Pomezia, Roma, Italy.
Felici
F
F
Galfre
G
G
Luzzago
A
A
Monaci
P
P
Nicosia
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001435:Bacteriophages / Q000235:genetics*; D000939:Epitopes / Q000032:analysis* / Q000235:genetics; D015723:Gene Library; D006801:Humans; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014612:Vaccines / Q000276:immunology
D000939:Epitopes; D011994:Recombinant Proteins; D014612:Vaccines