Detection of insulin-like growth factor binding proteins (IGFBPs) by ligand blotting in breast cancer tissues.
Eighty breast cancer specimens were examined for insulin-like growth factor binding protein (IGFBP) expression by ligand blotting. Five distinct IGFBP species were found: a doublet at 48 and 44 kDa was IGFBP-3, the 34-kDa band was IGFBP-2, and a band at 24 kDa was IGFBP-4. A 32-kDa band was compatible with the migration position reported for IGFBP-5. IGFBP-3 was inversely correlated with ER expression, while IGFBP-4 was positively correlated with both ER and PgR. IGFBP-4 was also inversely correlated with S-phase fraction. Thus, IGFBP expression correlates with other parameters of breast cancer biology and may play a role in regulating tumor growth.
McGuire
S E
SE
Department of Medicine, University of Texas Health Science Center at San Antonio 78284-7884.
Hilsenbeck
S G
SG
Figueroa
J A
JA
Jackson
J G
JG
Yee
D
D
D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D002352:Carrier Proteins / Q000032:analysis*; D002455:Cell Division; D005260:Female; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D013002:Somatomedins / Q000378:metabolism*
D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D013002:Somatomedins