title: In vitro proliferation by cells mobilized into the peripheral blood for collection and autologous transplantation.
abstract: We have investigated the properties of mobilized, cryopreserved, peripheral blood stem cells (PBSC), collected by leukapheresis over a period of 5 days, from eight myeloma patients in clinical remission. Cells were mobilized by treatment with cyclophosphamide and granulocyte colony-stimulating factor (G-CSF), and each day's collection was evaluated for its content of CD34+ cells, colony-forming units granulocyte/macrophage (CFU-GM), and plastic-adherent pre-CFU-GM. Peak values for these three parameters were observed at different times in different patients. There was no correlation between CD34+ content and CFU-GM, but there was some (r = 0.65) between CD34 numbers and colonies generated from a delta assay initiated using plastic-adherent pre-CFU-GM. In suspension cultures, the cells grew exponentially for 50 days. Thereafter, they did not divide, although they remained viable in culture for up to 1 month longer. Suspension cultures of PBSC grown with interleukin-3 (IL-3) displayed a predominantly myelomonocytic phenotype, but some megakaryocytes and erythroid cells were observed consistently. These results indicate that pre-CFU-GM in PBSC collections are capable of generating large numbers of clonogenic progeny in liquid culture and are capable of producing multiple lineages of differentiation.
authors.affiliation: Leukaemia Research Fund Centre, Chester Beatty Laboratories, London, UK.
authors.forename: L E
authors.initials: LE
authors.lastname: Healy
authors.forename: N
authors.initials: N
authors.lastname: Nirsimloo
authors.forename: M
authors.initials: M
authors.lastname: Scott
authors.forename: J F
authors.initials: JF
authors.lastname: Apperley
authors.forename: M Y
authors.initials: MY
authors.lastname: Gordon
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D002448:Cell Adhesion; D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003520:Cyclophosphamide / Q000494:pharmacology; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D016130:Immunophenotyping; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000494:pharmacology; D007937:Leukapheresis; D009101:Multiple Myeloma / Q000097:blood; D010969:Plastics
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D007377:Interleukin-3; D010969:Plastics; D016179:Granulocyte Colony-Stimulating Factor; D003520:Cyclophosphamide