Transplantation of CD34+ hematopoietic progenitor cells.
Sixty-six stage IV breast cancer patients received high dose chemotherapy followed by autologous transplantation of CD34-positive(+) cells obtained from the bone marrow and/or granulocyte colony stimulating factor (G-CSF)-mobilized peripheral blood. Grafts were examined for the presence of tumor using conventional histology and immunocytochemical staining. Patients achieved a granulocyte count of 500 x 10(9)/liter 10-12 days posttransplant, with a platelet count of > 20 x 10(9)/liter in 14-15 days. Enrichment of CD34+ cells from the peripheral blood progenitor cell (PBPC) collections resulted in a 1.3 to 4.0 log depletion of breast cancer cells from the graft.
Shpall
E J
EJ
Bone Marrow Transplant Program, University of Colorado, Denver.
Jones
R B
RB
Bearman
S I
SI
Purdy
M H
MH
Franklin
W A
WA
Heimfeld
S
S
Berenson
R J
RJ
D015703:Antigens, CD / Q000097:blood*; D018952:Antigens, CD34; D001943:Breast Neoplasms / Q000628:therapy*; D002469:Cell Separation; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans
D015703:Antigens, CD; D018952:Antigens, CD34