Renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmonid fish, detected by nested reverse transcription-PCR of 16S rRNA sequences.
An assay based on reverse transcription and nested PCR amplification of hypervariable regions within the 16S rRNA sequence was used to specifically detect Renibacterium salmoninarum, the slowly growing causative agent of bacterial kidney disease in salmonid fish. This assay detected 1 to 10 bacteria per sample and took 1 to 2 days to perform. The assay was used to detect R. salmoninarum in ovarian fluid obtained from naturally infected fish. The assay was unreliable when it was used to examine kidney tissue.
Magnússon
H B
HB
Institute for Experimental Pathology, University of Iceland, Reykjavík.
Fridjónsson
O H
OH
Andrésson
O S
OS
Benediktsdóttir
E
E
Gudmundsdóttir
S
S
Andrésdóttir
V
V
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D005393:Fish Diseases / Q000382:microbiology*; D005798:Genes, Bacterial / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000302:isolation & purification*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D007668:Kidney / Q000382:microbiology; D007674:Kidney Diseases / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D010053:Ovary / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012194:RNA-Directed DNA Polymerase; D012474:Salmon / Q000382:microbiology*; D012680:Sensitivity and Specificity
D012336:RNA, Ribosomal, 16S; D012194:RNA-Directed DNA Polymerase