Rapid microscopical diagnosis of deep-seated mycoses following maceration of fresh specimens and staining with optical brighteners.
A fluorescent staining procedure, which used to be recommended for the detection of fungi in skin and nail scrapings, can be adapted for the rapid detection of fungal elements in non-sectioned biopsies or respiratory secretions. The specimens are directly stained with an optical brightener (Blankophor P flüssig, Cellufluor, Uvitex 2B) preferentially after exposure to 20% aqueous potassium hydroxide solution. The procedure can also be applied to Gram-stained microscopic mounts. The high intensity of the fluorescence elicited allows rapid microscopic screening at low magnifications.
Rüchel
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R
Abteilung Medizinische Mikrobiologie, Universität Göttingen, Germany.
Margraf
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S
D005456:Fluorescent Dyes; D005658:Fungi / Q000166:cytology / Q000302:isolation & purification*; D006801:Humans; D006878:Hydroxides; D008856:Microscopy, Fluorescence / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis*; D009930:Organic Chemicals; D017680:Potassium Compounds; D013048:Specimen Handling; D013194:Staining and Labeling; D013267:Stilbenes; D014227:Triazines
D005456:Fluorescent Dyes; D006878:Hydroxides; D009930:Organic Chemicals; D017680:Potassium Compounds; D013267:Stilbenes; D014227:Triazines; C061166:fungiqual; C048787:cellufluor; C029943:potassium hydroxide