[Establishment and application of transplantable models of human esophageal carcinoma in nude mice].
We have successfully established two human esophageal carcinoma models in nude mice by transplantation of surgical specimen from patients with esophageal cancer. Histopathological study revealed that one was squamous cell carcinoma (HEC2) and the other adeno-squamous cell carcinoma (HEC6). Light and electron microscopy showed that their morphology was similar to that of the original tumors. The transplanted tumors have been maintained for more than four years through more than 50 passages. The tumors grew steadily and fast. The rate of successful inoculation was 100%. Bleomycin A5 and 5-Fluorouracil (5-Fu) were used for treatment of HEC6. Results showed that Bleomycin A5 exhibited a remarkable growth inhibition on the HEC6. The inhibition rates of two separate experiments were 56.5% and 87.8% respectively (compared with the control group, P < 0.01). The inhibition rates of 5-Fu were 6.5% and 36.6% respectively. Compared with the control group, there was no significant difference statistically HEC2 was treated by X-ray or X-ray combined with hematoporphyrin derivative (HPD), the tumor growth inhibition was 57.7% and 85.4% respectively.
Cai
H Y
HY
Cancer Center, Sun Yet-sen University of Medical Sciences, Guangzhou, Guangdong.
D000818:Animals; D001761:Bleomycin / Q000008:administration & dosage / Q000031:analogs & derivatives; D018196:Carcinoma, Adenosquamous / Q000188:drug therapy / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000473:pathology*; D004195:Disease Models, Animal; D004938:Esophageal Neoplasms / Q000188:drug therapy / Q000473:pathology*; D005472:Fluorouracil / Q000008:administration & dosage; D017324:Hematoporphyrin Derivative / Q000627:therapeutic use; D006414:Hematoporphyrin Photoradiation; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation
D001761:Bleomycin; C025703:bleomycetin; D017324:Hematoporphyrin Derivative; D005472:Fluorouracil