title: Aneuploidy and aneusomy of chromosome 7 detected by fluorescence in situ hybridization are markers of poor prognosis in prostate cancer.
abstract: Fluorescence in situ hybridization is a new methodology which can be used to detect cytogenetic anomalies within interphase tumor cells. We used this technique to identify nonrandom numeric chromosomal alterations in tumor specimens from the poorest prognosis patients with pathological stages T2N0M0 and T3N0M0 prostate carcinomas. Among 1368 patients treated by radical prostatectomy, 25 study patients were ascertained who died most quickly from progressive prostate carcinoma within 3 years of diagnosis and surgery. Tumors from 25 control patients who survival for more than 5 years and who were matched for age, tumor histological grade, and pathological stage also were evaluated. The tumors from all 25 (100%) poor prognosis patients and from 11 of 25 (44%) control patients were found to be aneuploid by fluorescence in situ hybridization (P < 0.0001). Alterations of chromosome 7 were observed in 24 of the tumors (96%) from the poor prognosis patients versus 3 tumors (12%) from the control group (P < 0.0001). Moreover, a characteristic aneuploidy pattern with multiple abnormal chromosomes and a hypertetrasomic population was generally found in tumors from the poor prognosis patients. This preliminary study suggests that fluorescence in situ hybridization studies of prostate cancer specimens may help to identify those patients at highest risk for early cancer death.
authors.affiliation: Department of Urology, Mayo Clinic, Rochester, Minnesota 55905.
authors.forename: A
authors.initials: A
authors.lastname: Alcaraz
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: J A
authors.initials: JA
authors.lastname: Brown
authors.forename: J F
authors.initials: JF
authors.lastname: Herath
authors.forename: E J
authors.initials: EJ
authors.lastname: Bergstralh
authors.forename: J J
authors.initials: JJ
authors.lastname: Larson-Keller
authors.forename: M M
authors.initials: MM
authors.lastname: Lieber
authors.forename: R B
authors.initials: RB
authors.lastname: Jenkins
mesh_terms: D000368:Aged; D000782:Aneuploidy; D016022:Case-Control Studies; D002886:Chromosomes, Human, Pair 17; D002897:Chromosomes, Human, Pair 7; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D011003:Ploidies; D011379:Prognosis; D011470:Prostatic Hyperplasia / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000401:mortality; D012016:Reference Values; D012730:Sex Chromosomes
chemical_list: D005819:Genetic Markers