title: Characterization of a voltage-dependent anionic channel in fused synaptosomes isolated from rat hippocampi.
abstract: The inside out configuration of the patch-clamp technique was used to study single-channel anionic currents from purified hippocampal synaptosomes fused into liposomes to form giant proteoliposomes. At least six different anionic channels with unitary conductances of 22-150 pS were found. The most frequently observed was the 32-pS conductance channel. This was voltage-dependent; the open probability increased from 0.20 at -40 mV to 0.46 at 40 mV. This channel may be involved in the repolarization of nerve terminal membranes after an action potential, thus, limiting the duration of the spike and the transmitter release.
authors.affiliation: Biophysics Laboratory, International School for Advanced Studies, Trieste, Italy.
authors.forename: Y
authors.initials: Y
authors.lastname: Hosokawa
authors.forename: G
authors.initials: G
authors.lastname: Sandri
authors.forename: E
authors.initials: E
authors.lastname: Panfili
authors.forename: E
authors.initials: E
authors.lastname: Cherubini
mesh_terms: D000818:Animals; D000838:Anions / Q000378:metabolism; D004594:Electrophysiology; D006624:Hippocampus / Q000378:metabolism* / Q000648:ultrastructure; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000378:metabolism*
chemical_list: D000838:Anions; D007473:Ion Channels; D008081:Liposomes