Role of alpha 3 beta 1 and alpha 2 beta 1 integrins in melanoma cell migration.
Recent findings indicate that variable expression of beta 1 integrins may play a role in differential melanoma cell motility. Primary melanoma (PM) and metastatic melanoma (MM) cultures, derived from the same patient, were tested for their beta 1, alpha 2, alpha 3 and alpha 6 integrin subunit expression and cell migration on type IV collagen (CN IV) or laminin (LN). The MM cell line expressed markedly increased levels of the beta 1, alpha 2 and alpha 3, but not alpha 6 subunit compared to the PM cell line. The MM cell migration rate was significantly higher than that of the PM cell line on LN- or CN IV-coated substrates. Furthermore, the cell migration rate of both lines was significantly higher (p < 0.001) on these substrates than on the control substrates. The MM and PM cell migration was significantly inhibited by function-blocking anti-beta 1 and anti-alpha 3 MAbs, but not by the anti-alpha 6 MAb tested. In contrast, the anti-alpha 2 MAb significantly inhibited MM but not PM cell migration. These data show that the alpha 3 subunit plays a significant role in melanoma cell motility on CN IV and LN and that the alpha 2 subunit has a significant contribution to the motility of the MM cell line.
Yoshinaga
I G
IG
Department of Pathology, Harvard Medical School, Massachusetts General Hospital, Boston.
Vink
J
J
Dekker
S K
SK
Mihm
M C
MC
Byers
H R
HR
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD / Q000502:physiology*; D002448:Cell Adhesion; D002465:Cell Movement; D003094:Collagen; D006801:Humans; D039201:Integrin alpha3beta1; D019012:Integrin beta1; D016023:Integrins / Q000037:antagonists & inhibitors / Q000502:physiology*; D007797:Laminin; D008207:Lymphatic Metastasis / Q000473:pathology*; D008545:Melanoma / Q000473:pathology*; D009363:Neoplasm Proteins / Q000037:antagonists & inhibitors / Q000502:physiology*; D012878:Skin Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D039201:Integrin alpha3beta1; D019012:Integrin beta1; D016023:Integrins; D007797:Laminin; D009363:Neoplasm Proteins; D003094:Collagen