Application of dual parameter analysis in flow cytometric DNA measurements of paraffin-embedded samples.
In a comparison of flow cytometric DNA measurements on fresh and paraffin-embedded material from primary squamous cell carcinomas of the head and neck region, we discovered that previously undetected aneuploid clones could be detected by dual parameter analysis of cytokeratin and DNA applied to disintegrated cells from paraffin sections. Using this new approach the correlation coefficient between DNA-indices from fresh and paraffin-embedded material increased from 0.423 to 0.904.
Nylander
K
K
Department of Oral Pathology, University of Umeå, Sweden.
Stenling
R
R
Gustafsson
H
H
Roos
G
G
D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis*; D004848:Epithelium / Q000737:chemistry; D005404:Fixatives; D005434:Flow Cytometry / Q000379:methods; D005557:Formaldehyde; D016193:G1 Phase; D006258:Head and Neck Neoplasms / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D007633:Keratins / Q000032:analysis*; D016612:Paraffin Embedding; D016192:Resting Phase, Cell Cycle
D004273:DNA, Neoplasm; D005404:Fixatives; D005557:Formaldehyde; D007633:Keratins