title: Generation and characterization of a human monoclonal IgG antibody specific for HLA-B12.
abstract: We describe here the generation and characterization of a human monoclonal IgG antibody (UL/F14) specific for HLA-B12. The antibody is suitable for complement-dependent lysis on lymphoblastoid cell lines and stimulated peripheral blood mononuclear cells. UL/F14 competes for antigen binding with an HLA-B12 human monoclonal IgM antibody and with a specific alloantiserum. Protein chemistry shows that the monoclonal antibody UL/F14 can precipitate solubilized HLA-B12 molecules.
authors.affiliation: Department of Transplantation Immunology, Red Cross Blood Bank, Ulm, FRG.
authors.forename: K
authors.initials: K
authors.lastname: Siemoneit
authors.forename: A
authors.initials: A
authors.lastname: Wölpl
authors.forename: S
authors.initials: S
authors.lastname: Wegener
authors.forename: M
authors.initials: M
authors.lastname: Ernst
authors.forename: H H
authors.initials: HH
authors.lastname: Sonneborn
authors.forename: S F
authors.initials: SF
authors.lastname: Goldmann
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D001667:Binding, Competitive; D002461:Cell Line, Transformed; D003165:Complement System Proteins / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D015235:HLA-B Antigens / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000276:immunology; D008213:Lymphocyte Activation; D011233:Precipitin Tests
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D015235:HLA-B Antigens; C035015:HLA-B12 antigen; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003165:Complement System Proteins