title: Trisomy 7: a potential cytogenetic marker of human prostate cancer progression.
abstract: We used the fluorescence in situ hybridization (FISH) method to show that chromosome 7 trisomy is associated with the progression of human prostate cancer. Thirty-six specimens including 15 primary prostate carcinomas, 16 metastatic lesions, and 5 normal prostate tissues, as well as 2 prostate carcinoma cell lines of different tumorigenic potential, were examined for chromosome 7 aneuploidy. Our results showed that the androgen-unresponsive tumorigenic cell line PC-3 exhibited a significantly higher ratio of chromosome 7 to total chromosome number than the androgen-responsive nontumorigenic cell line LNCaP (P = 0.001). In prostate specimens, the frequency of trisomy 7 cells was significantly increased (P < 0.05) in the advanced stage tumors (C and DI) but not in the early (B) stage tumors or normal prostatic tissue. Furthermore, metastases showed a higher frequency of trisomy 7 cells than primary tumors (P = 0.005). In 2 patients with paired primary and metastatic tumors, trisomy 7 cells increased from 4-7% in the primary tumors to 42-45% in the metastatic tumor cells in the bone marrow. Therefore, our data suggest that trisomy 7 may be a common feature associated with local and metastatic progression and serve as a novel marker for human prostate cancer progression.
authors.affiliation: Department of Urology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
authors.forename: M G
authors.initials: MG
authors.lastname: Bandyk
authors.forename: L
authors.initials: L
authors.lastname: Zhao
authors.forename: P
authors.initials: P
authors.lastname: Troncoso
authors.forename: L L
authors.initials: LL
authors.lastname: Pisters
authors.forename: J L
authors.initials: JL
authors.lastname: Palmer
authors.forename: A C
authors.initials: AC
authors.lastname: von Eschenbach
authors.forename: L W
authors.initials: LW
authors.lastname: Chung
authors.forename: J C
authors.initials: JC
authors.lastname: Liang
mesh_terms: D002897:Chromosomes, Human, Pair 7; D002899:Chromosomes, Human, Pair 9; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D014314:Trisomy; D014407:Tumor Cells, Cultured
chemical_list: D005819:Genetic Markers