Mapping the lipoyl groups of the pyruvate dehydrogenase complex by use of gold cluster labels and scanning transmission electron microscopy.
This paper describes the organization of lipoyl moieties within the pyruvate dehydrogenase (PDH) complex from Escherichia coli as studied in the scanning transmission electron microscope (STEM). The PDH complex is a multienzyme complex consisting of E1, pyruvate dehydrogenase, E2, dihydrolipoyl transacetylase, and E3, dihydrolipoyl dehydrogenase. The core of the complex is the cubic 24-subunit E2 component, which contains the lipoyl moieties bonded to lipoyl-bearing domains. E1 and E3 are associated along the edges (E1) and on the faces (E3) of the core. The lipoyl moieties were reduced with NADH and alkylated with a p-maleimidobenzoyl undecagold cluster complex. The gold labels were found to be bound very nearly specifically by dihydrolipoyl transacetylase (E2). Undecagold clusters were imaged directly by the STEM and also digitally mapped by radial mass analysis. The mass of the E2E3 subcomplex is about half that of the PDH complex. The PDH complex and GC-PDH are both about 420 A in diameter, as determined by radial mass analysis, and the E2E3 subcomplex and GC-E2E3 are 320 and 350 A, respectively. The outer boundary of the E2E3 subcomplex was clearly shown in STEM micrographs by the undecagold labels in GC-E2E3. Data obtained from radial mass analysis of GC-E2E3 and the unlabeled E2E3 subcomplex also showed that the size of the subcomplex is extended by the lipoyl-bearing domains surrounding the central E2 core. The capabilities of lipoyl moieties to undergo translocation over long distances through structural mobility in the lipoyl-bearing domains was confirmed by the observation that many of the lipoyl groups in E2E3 subcomplexes relax outward into space vacated by the removal of E1 during the preparation of the subcomplex from PDH complex. Radial mass analysis of the PDH complex and GC-PDH indicates that lipoyl groups are distributed over a large region of the PDH complex, extending from the central core to 170-180 A from the center of the complex, with the highest density at about 75 A from the particle centers, near the interface between E2 and the associated components E1 and E3.
Yang
Y S
YS
Institute for Enzyme Research, Graduate School, University of Wisconsin, Madison 53705.
Datta
A
A
Hainfeld
J F
JF
Furuya
F R
FR
Wall
J S
JS
Frey
P A
PA
D004926:Escherichia coli / Q000201:enzymology*; D006046:Gold; D007091:Image Processing, Computer-Assisted; D017348:Microscopy, Electron, Scanning Transmission; D015335:Molecular Probes; D050607:Organogold Compounds; D009942:Organometallic Compounds; D011768:Pyruvate Dehydrogenase Complex / Q000737:chemistry / Q000648:ultrastructure*; D013194:Staining and Labeling; D008063:Thioctic Acid / Q000737:chemistry / Q000302:isolation & purification*
D015335:Molecular Probes; D050607:Organogold Compounds; D009942:Organometallic Compounds; D011768:Pyruvate Dehydrogenase Complex; C035797:undecagold; D008063:Thioctic Acid; D006046:Gold