"Stimulation of dihydropyridine-sensitive Ca2+ influx in cultured myoblasts by 1,25(OH)2-vitamin D3".
The rapid, non-genomic effects of 1,25(OH)2-vitamin D3 [1,25(OH)2D3] on calcium influx in cultured neonate rat and embryonic chick myoblasts were investigated. The hormone stimulated 45Ca uptake in a time (1-10 min) and dose (10(-11)-10(-7) M)-dependent manner. The effects of 1,25(OH)2D3 on both rat and chick myoblasts were mimicked by the Ca(2+)-channel agonist BAY K8644 and effectively suppressed by the antagonist nifedipine. 1,25(OH)2D3 induction of Ca2+ uptake was dependent on the presence of extracellular calcium, since it was abolished by prior addition of EGTA and was restored upon the addition of 1 mM Ca2+ Cell membrane depolarization with high K+, similarly to the hormone, elicited a rapid increase in Ca2+ uptake (+75% above control). These results suggest the modulation of Ca2+ influx through competent calcium channels in rat and chick embryo myoblasts by 1,25(OH)2D3.
Vazquez
G
G
Depto. Biologia, Universidad Nacional del Sur, Bahia Blanca, Argentina.
de Boland
A R
AR
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D002117:Calcitriol / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects*; D002478:Cells, Cultured; D002642:Chick Embryo; D004095:Dihydropyridines / Q000494:pharmacology*; D004533:Egtazic Acid / Q000494:pharmacology; D009132:Muscles / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009543:Nifedipine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D015220:Calcium Channels; D004095:Dihydropyridines; D004533:Egtazic Acid; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; C038806:1,4-dihydropyridine; D002117:Calcitriol; D009543:Nifedipine