Interactions of atrial natriuretic peptide with A7r5 cells.
The interactions of Atrial Natriuretic Peptide with its receptors were investigated with the vascular smooth muscle cells from the A7r5 cell line. Displacement experiments of [125I]rat-Atrial Natriuretic Peptide by rat-Atrial Natriuretic Peptide revealed a single class of receptors with a Kd = 0.21 +/- 0.08 nM and a Bmax = of 35 +/- 16 fmol/mg of protein. Furthermore, the complex [125I]r-Atrial Natriuretic Peptide was internalised by a heatsensitive process. Finally, Atrial Natriuretic Peptide increased cyclic Guanosyl 3',5' Mono Phosphate in a time dependent and dose dependent way, a concentration of 0.1 microM increasing cyclic Guanosyl 3',5' Mono Phosphate level by a factor of 8.3 times when compared to basal level.
Aptel
H B
HB
Sheffield University, Department of Animal and Plant Sciences, Western Bank, England.
Deacon
C F
CF
Henderson
I W
IW
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism* / Q000494:pharmacology*; D001414:Bacitracin / Q000494:pharmacology; D001667:Binding, Competitive; D002460:Cell Line; D006152:Cyclic GMP / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor / Q000378:metabolism*; D013997:Time Factors
D007457:Iodine Radioisotopes; D001414:Bacitracin; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP; D015056:1-Methyl-3-isobutylxanthine