Behavior of embryonic chick heart cells in culture. 3. Spatial distribution of epidermal growth factor in heart muscle cells.
Muscle cell-enriched primary cell cultures were prepared from 8-day embryonic chick heart ventricles. Immunostaining with an anti-EGF antibody detected the presence of EGF in the heart muscle cells, in a perinuclear distribution. In addition, some of the EGF appeared to be associated with the sarcomeres. Moreover, immunostaining with an anti-EGF receptor antibody also detected perinuclear localization, albeit not among the sarcomeres. Previous studies have shown that the heart muscle and non-muscle cells in culture respond to EGF to increase [3H]thymidine incorporation and cell division (Lau, 1993b). These data suggest the roles of EGF as a natural regulator in the development of the embryonic chick heart.
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Division of Biology, Kansas State University, Manhattan 66506.
D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D002642:Chick Embryo; D004815:Epidermal Growth Factor / Q000032:analysis*; D066246:ErbB Receptors / Q000032:analysis*; D009206:Myocardium / Q000737:chemistry* / Q000166:cytology; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D004815:Epidermal Growth Factor; D066246:ErbB Receptors