Molecular cloning and characterization of G-CSF induced gene cDNA.
G-CSF (granulocyte colony-stimulating factor) is known to specifically stimulate the production and the functional activation of neutrophils. To investigate the intracellular signaling pathway of myeloid cells stimulated by G-CSF, we isolated new genes whose expression was induced by G-CSF. First of all, we constructed lambda gt10 cDNA library from G-CSF-stimulated mononuclear cells (MNC) of a chronic myelogenous leukemia (CML) patient (CML-MNC) and screened the cDNA library by a differential hybridization method. The 24 candidate clones which specifically hybridized with G-CSF-stimulated CML-MNC cDNA probes, but not with unstimulated CML-MNC cDNA probes, were obtained after 8 x 10(4) individual clones had been screened. One of these clones, GIG-1 (G-CSF-induced gene-1), was further characterized. The size of the GIG-1 mRNA was about 0.9kb. The GIG-1 mRNA was expressed mainly in the myeloid leukemic cell lines.
Shimane
M
M
Institute of Medical Science, University of Tokyo, Japan.
Tani
K
K
Maruyama
K
K
Takahashi
S
S
Ozawa
K
K
Asano
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D005796:Genes; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000473:pathology; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D039102:Poly(A)-Binding Proteins; D011506:Proteins; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D015183:Restriction Mapping; D012995:Solubility; D000074019:T-Cell Intracellular Antigen-1
D017931:DNA Primers; D008565:Membrane Proteins; D039102:Poly(A)-Binding Proteins; D011506:Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D000074019:T-Cell Intracellular Antigen-1; C071558:TIA1 protein, human; D016179:Granulocyte Colony-Stimulating Factor