Identification of subspecies- and serotype 1-specific epitopes on the 80- to 90-kilodalton protein region of Chlamydia psittaci that may be useful for diagnosis of chlamydial induced abortion.
Genus-, subspecies-, and serotype 1-specific antigens of Chlamydia psittaci were characterized by immunoblot analysis, using monoclonal antibodies that recognize 2 C psittaci strains: AB7 isolated from an ewe that had aborted, and iB1 isolated from feces of a healthy ewe. Genus-specific epitopes were detected on lipopolysaccharide, on a 47-kd protein, and on a 27- to 30-kd doublet. Subspecies-specific epitopes were located on a 30-kd protein, and a 80- to 90-kd protein region was identified, which bore subspecies- and serotype 1-specific epitopes. These 80- to 90-kd proteins were highly reactive with serum from ewes that had aborted and could be a useful antigen for diagnosis of chlamydial induced abortion of ruminants.
Souriau
A
A
INRA Nouzilly, France.
Salinas
J
J
De Sa
C
C
Layachi
K
K
Rodolakis
A
A
D000034:Abortion, Veterinary / Q000175:diagnosis* / Q000382:microbiology; D000818:Animals; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000276:immunology*; D002690:Chlamydia Infections / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D002691:Chlamydophila psittaci / Q000145:classification / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D011247:Pregnancy; D012703:Serotyping; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000382:microbiology; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D000939:Epitopes