Receptors for peptide YY and neuropeptide Y on guinea pig pancreatic acini.
We examined the effects of peptide YY (PYY), neuropeptide Y (NPY), and their analogues on dispersed pancreatic acini. Binding of [125I]PYY to acini was saturable, reversible, and specific, and PYY binding was best fit with a two-site model. The relative potencies for inhibiting [125I]PYY binding were PYY > or = NPY > NPY(13-36). There was no inhibition of binding with [Leu31,Pro34]NPY, PYX-2, or pancreatic polypeptide. Both PYY and NPY (0.1 microM) inhibited amylase release stimulated by vasoactive intestinal polypeptide (VIP) (0.3 nM) and forskolin (1 microM) by about 30%, but not that stimulated by cholecystokinin-8 or bombesin. The relative potencies for inhibiting VIP-stimulated amylase release were PYY > or = NPY > NPY(13-36), the same as those for inhibiting VIP-stimulated cAMP increase in acini. No inhibition was detected with [Leu31,Pro34]NPY. This work demonstrates Y2 receptors on guinea pig pancreatic acini mediating inhibitory actions of PYY and NPY on pancreatic enzyme secretion.
Huang
S C
SC
Department of Physiology, national Taiwan University, Taipei.
Tsai
M F
MF
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis / Q000378:metabolism*; D005768:Gastrointestinal Hormones / Q000494:pharmacology; D006168:Guinea Pigs; D008297:Male; D009478:Neuropeptide Y / Q000494:pharmacology; D010179:Pancreas / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D019894:Peptide YY; D010455:Peptides / Q000494:pharmacology; D011869:Radioligand Assay; D011964:Receptors, Gastrointestinal Hormone / Q000032:analysis*; D017476:Receptors, Neuropeptide Y / Q000032:analysis*; D014660:Vasoactive Intestinal Peptide / Q000037:antagonists & inhibitors
D005768:Gastrointestinal Hormones; D009478:Neuropeptide Y; D010455:Peptides; D011964:Receptors, Gastrointestinal Hormone; D017476:Receptors, Neuropeptide Y; C082172:peptide YY receptor; D019894:Peptide YY; D014660:Vasoactive Intestinal Peptide; D000242:Cyclic AMP; D000681:Amylases