Evaluation of indeterminate sera for hepatitis C virus by assays using synthetic peptides.
The development of new immunodiagnostic systems to study antibodies anti-HCV based on the use of synthetic peptides are potentially very important in the evaluation of indeterminate samples. We have compared two immunodiagnostic tests, the 3-RIBA and LIA-HCV test system, to examine the samples rated as indeterminate by the 2-RIBA test. The results showed that the specificity of the new tests has improved, in fact 29/40 of the indeterminate samples studied became positive with both tests. However, the 3-RIBA test showed higher sensitivity, since two samples negative by LIA-HCV, were positive with this system.
Rivanera
D
D
Istituto di Microbiologia, Facoltà di Medicina e Chirurgia La Sapienza, Rome, Italy.
Lorino
G
G
Lilli
D
D
Dicuonzo
G
G
Mancini
C
C
D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D016174:Hepacivirus / Q000276:immunology*; D006508:Hepatitis Antibodies / Q000097:blood*; D006526:Hepatitis C / Q000097:blood* / Q000453:epidemiology; D018937:Hepatitis C Antibodies; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D010455:Peptides; D012680:Sensitivity and Specificity
D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D010455:Peptides