Association of the GPI-anchored leucocyte surface glycoproteins with ganglioside GM3.
The major glycolipid co-immunopurifying with the glycosylphosphatidylinositol-anchored leucocyte surface glycoprotein CD59 from detergent lysates of human T cell lines HPB ALL, Jurkat and myeloid line HL-60 was identified as the glycosphingolipid GM3. Monoclonal antibodies to GM3 immunoprecipitated the same large detergent-resistant, protein-tyrosine kinase containing "GPI-complexes" as antibodies to several GPI-anchored proteins. Therefore GM3 is another component of these large membrane complexes potentially involved in signalling through GPI-anchored receptors or through some glycolipids.
Kniep
B
B
GBF, Immunobiology Group, Braunschweig, Germany.
Cinek
T
T
Angelisová
P
P
Horejsí
V
V
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D018957:CD59 Antigens; D005679:G(M3) Ganglioside / Q000032:analysis / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols / Q000032:analysis / Q000378:metabolism*; D006098:Granulocytes / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007163:Immunosorbent Techniques; D008562:Membrane Glycoproteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000737:chemistry / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018957:CD59 Antigens; D005679:G(M3) Ganglioside; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins