Requirement of vascular integrin alpha v beta 3 for angiogenesis.
Angiogenesis depends on the adhesive interactions of vascular cells. The adhesion receptor integrin alpha v beta 3 was identified as a marker of angiogenic vascular tissue. Integrin alpha v beta 3 was expressed on blood vessels in human wound granulation tissue but not in normal skin, and it showed a fourfold increase in expression during angiogenesis on the chick chorioallantoic membrane. In the latter assay, a monoclonal antibody to alpha v beta 3 blocked angiogenesis induced by basic fibroblast growth factor, tumor necrosis factor-alpha, and human melanoma fragments but had no effect on preexisting vessels. These findings suggest that alpha v beta 3 may be a useful therapeutic target for diseases characterized by neovascularization.
Brooks
P C
PC
Department of Immunology, Scripps Research Institute, La Jolla, CA 92037.
Clark
R A
RA
Cheresh
D A
DA
D000818:Animals; D000911:Antibodies, Monoclonal; D001808:Blood Vessels / Q000378:metabolism; D002642:Chick Embryo; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006097:Granulation Tissue / Q000098:blood supply* / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D007797:Laminin / Q000032:analysis; D008545:Melanoma / Q000098:blood supply / Q000378:metabolism; D009389:Neovascularization, Pathologic / Q000378:metabolism*; D016024:Receptors, Cytoadhesin / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D019030:Receptors, Vitronectin; D012867:Skin / Q000098:blood supply / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014841:von Willebrand Factor / Q000032:analysis
D000911:Antibodies, Monoclonal; D016023:Integrins; D007797:Laminin; D016024:Receptors, Cytoadhesin; D019030:Receptors, Vitronectin; D014409:Tumor Necrosis Factor-alpha; D014841:von Willebrand Factor; D016222:Fibroblast Growth Factor 2