Membrane topology of the GluR1 glutamate receptor subunit: epitope mapping by site-directed antipeptide antibodies.
In order to define the membrane topology of the GluR1 glutamate receptor subunit, we have examined the location of epitopes. Antibodies were produced against peptides corresponding to putative extracellular and intracellular segments of the rat brain GluR1 glutamate receptor subunit. Immunocytochemistry at the electron microscopic level in the dentate gyrus of the hippocampal formation showed that epitopes for the antiserum to the N-terminal part of the subunit are located at the extracellular face of the plasma membrane, whereas the antigenic determinants for the antiserum to the C-terminal part are found at the intracellular face of the postsynaptic membrane. Furthermore, antibodies to the N-terminal residues 253-267 reacted similarly with both intact and permeabilized synaptosomes, whereas the binding of antibodies to the C-terminal residues 877-889 increased about 1.6-fold following permeabilization. Our data suggest that the N- and C-terminal regions are located on the opposite side of the membrane and, therefore, the GluR1 subunit probably has an odd number of membrane spanning segments. The antibody cross-reactivities in different species and their effect on ligand binding activity were also established.
Molnár
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E
Medical Research Council, Anatomical Neuropharmacology Unit, University of Oxford, England.
McIlhinney
R A
RA
Baude
A
A
Nusser
Z
Z
Somogyi
P
P
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001921:Brain / Q000378:metabolism*; D010856:Columbidae; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D005260:Female; D005289:Ferrets; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007608:Kainic Acid / Q000378:metabolism; D046911:Macromolecular Substances; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D017470:Receptors, Glutamate / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D013045:Species Specificity; D013574:Synaptosomes / Q000378:metabolism* / Q000648:ultrastructure; D014316:Tritium; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000378:metabolism
D000906:Antibodies; D000939:Epitopes; D046911:Macromolecular Substances; D010455:Peptides; D017470:Receptors, Glutamate; D014316:Tritium; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D007608:Kainic Acid