Effects of hormones, cyclic AMP analogues and growth factors on steel factor (SF) production in mouse Sertoli cell cultures.
Regulation of steel factor (SF) production in Sertoli cells from postnatal mouse testes was studied in a mast cell-Sertoli cell coculture system. Treatment of Sertoli cells with dibutyryl cAMP (50-1000 mumol l-1), forskolin (1-25 mmol l-1), and cholera toxin (10 micrograms ml-1) increased SF production, whereas FSH and theophylline had no significant effect. Furthermore, growth factors and testosterone, which would play some roles in spermatogenesis, were also tested, but none of these stimulated SF production. The constitutive production of SF may, rather, reflect the physiological condition of Sertoli cells in vivo.
Tajima
Y
Y
Research Institute for Microbial Diseases, Osaka University, Japan.
Nishina
Y
Y
Koshimizu
U
U
Jippo
T
T
Kitamura
Y
Y
Nishimune
Y
Y
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology*; D005576:Colforsin / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D016298:Hematopoietic Cell Growth Factors / Q000096:biosynthesis*; D008297:Male; D008407:Mast Cells / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D019089:Stem Cell Factor; D013268:Stimulation, Chemical; D013806:Theophylline / Q000494:pharmacology
D016298:Hematopoietic Cell Growth Factors; D019089:Stem Cell Factor; D005576:Colforsin; D003994:Bucladesine; D005640:Follicle Stimulating Hormone; D002772:Cholera Toxin; D013806:Theophylline