title: Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli.
abstract: Reverse transcriptase from feline immunodeficiency virus (FIV) has been cloned and expressed in Escherichia coli. We have purified this recombinant enzyme and shown that it is a 66-kDa protein that is indistinguishable from virion-derived FIV reverse transcriptase in sensitivity to the 5'-triphosphates of 3'-azido-3'-deoxythymidine and the four 2',3'-dideoxynucleosides. The availability of large quantities of the FIV reverse transcriptase will allow more detailed physical and pharmacological studies.
authors.affiliation: Division of Biological Sciences, University of Montana, Missoula 59812.
authors.forename: T W
authors.initials: TW
authors.lastname: North
authors.forename: G L
authors.initials: GL
authors.lastname: Hansen
authors.forename: Y
authors.initials: Y
authors.lastname: Zhu
authors.forename: J A
authors.initials: JA
authors.lastname: Griffin
authors.forename: C K
authors.initials: CK
authors.lastname: Shih
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D016089:Immunodeficiency Virus, Feline / Q000201:enzymology* / Q000235:genetics*; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D014771:Virion / Q000201:enzymology
chemical_list: D004279:DNA, Viral; D011994:Recombinant Proteins; D012194:RNA-Directed DNA Polymerase