A hyperpolarization-activated cation conductance in lobster olfactory receptor neurons.
1. The current underlying inward rectification in lobster olfactory receptor neurons was investigated with the use of whole-cell patch-clamp techniques. Inward rectification could most likely result from an inwardly rectifying potassium conductance or a hyperpolarization-activated cation conductance. To distinguish between these possibilities, the current underlying inward rectification was examined with respect to its sensitivity to extracellular Cs+ and Ba2+, time course of activation, and reversal potential. 2. In current clamp, injection of negative current led to a hyperpolarization followed by a partial return (sag) toward the initial holding potential. The rate and magnitude of the sag depended on the magnitude of the hyperpolarizing current with larger currents leading to larger, faster depolarizing sags. In voltage clamp, hyperpolarizing steps elicited a slowly activating, noninactivating inward current clamp. Both the sag and the slow inward current were blocked reversibly by extracellular application of 5 mM CsCl but were unaffected by 2 mM BaCl2. 3. The rate of inward current activation was best approximated by a single exponential function with time constants that were voltage dependent, ranging from 7.8 s at -69 mV to 248 ms at -114 mV. 4. Cells normally exhibited an average input resistance of 0.99 G omega over the range of -69 to -114 mV. With the hyperpolarization-activated inward current blocked by 5 mM CsCl, the average input resistance increased to 2.12 G omega over the same range. 5. Analysis of tail currents revealed that the average predicted reversal potential of the hyperpolarization-activated inward current was 1.7 mV and was not affected significantly by a shift in ECl.(ABSTRACT TRUNCATED AT 400 WORDS)
Corotto
F S
FS
Department of Physiology, University of Utah School of Medicine, Salt Lake City 84108.
Michel
W C
WC
D000818:Animals; D001464:Barium / Q000502:physiology; D002412:Cations / Q000378:metabolism*; D002478:Cells, Cultured; D002586:Cesium / Q000494:pharmacology; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008121:Nephropidae / Q000502:physiology*; D018034:Olfactory Receptor Neurons / Q000187:drug effects / Q000502:physiology*; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology*
D002412:Cations; D007473:Ion Channels; D002586:Cesium; D001464:Barium