title: Cell-autonomous Fas (CD95)/Fas-ligand interaction mediates activation-induced apoptosis in T-cell hybridomas.
abstract: A number of murine T-cell hybridomas undergo apoptosis within a few hours of activation by specific antigens, mitogens, antibodies against the T-cell antigen receptor, or a combination of phorbol ester and calcium ionophore. This phenomenon has been extensively studied as a model for clonal deletion in the immune system, in which potentially autoreactive T cells eliminate themselves by apoptosis after activation, either in the thymus or in the periphery. Here we show that the Fas/CD95 receptor, which can transduce a potent apoptotic signal when ligand, is rapidly expressed following activation of T-cell hybridomas, as is its functional, membrane-bound ligand. Interference with the ensuing Fas/Fas-ligand interaction inhibits activation-induced apoptosis. Because T-cell receptor ligation can induce apoptosis in a single T hybridoma cell, we suggest that the Fas/Fas-ligand interaction can induce cell death in a cell-autonomous manner.
authors.affiliation: Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, California 92037.
authors.forename: T
authors.initials: T
authors.lastname: Brunner
authors.forename: R J
authors.initials: RJ
authors.lastname: Mogil
authors.forename: D
authors.initials: D
authors.lastname: LaFace
authors.forename: N J
authors.initials: NJ
authors.lastname: Yoo
authors.forename: A
authors.initials: A
authors.lastname: Mahboubi
authors.forename: F
authors.initials: F
authors.lastname: Echeverri
authors.forename: S J
authors.initials: SJ
authors.lastname: Martin
authors.forename: W R
authors.initials: WR
authors.lastname: Force
authors.forename: D H
authors.initials: DH
authors.lastname: Lynch
authors.forename: C F
authors.initials: CF
authors.lastname: Ware
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000502:physiology*; D017209:Apoptosis / Q000502:physiology*; D001483:Base Sequence; D004247:DNA; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D006825:Hybridomas; D008024:Ligands; D008213:Lymphocyte Activation; D051379:Mice; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology*; D019014:fas Receptor
chemical_list: D000954:Antigens, Surface; D008024:Ligands; D011993:Recombinant Fusion Proteins; D019014:fas Receptor; D004247:DNA; D010450:Endopeptidases