Nature of the reactive epitopes in Paracoccidioides brasiliensis polysaccharide antigen.
Fava Nettos' polysaccharide antigen (FNPA), shown to detect humoral and cellular responses in paracoccidioidomycosis, was investigated. Skin tests with FNPA were negative after alkaline hydrolysis or depletion of gp43 peptide epitopes. Purified antibodies to FNPA or gp43 from paracoccidioidomycosis patients cross-reacted, showing common epitopes and FNPA-specific ones. Normal human sera, in contrast to paracoccidioidomycosis sera, were unreactive with gp43 but recognized epitopes of FNPA susceptible to alkaline hydrolysis and pronase treatment. Histoplasmosis patients sera strongly reacted with FNPA carbohydrate epitopes.
Rodrigues
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Disciplina de Biologia Celular, Escola Paulista de Medicina, São Paulo, Brazil.
Travassos
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LR
D000818:Animals; D000908:Antibodies, Fungal; D000946:Antigens, Fungal / Q000276:immunology* / Q000302:isolation & purification; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D006168:Guinea Pigs; D006660:Histoplasmosis / Q000276:immunology; D006801:Humans; D008297:Male; D010228:Paracoccidioides / Q000276:immunology*; D010229:Paracoccidioidomycosis / Q000276:immunology*; D011134:Polysaccharides / Q000276:immunology* / Q000302:isolation & purification; D012882:Skin Tests
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D000939:Epitopes; D011134:Polysaccharides