A common T-cell epitope between human thyroglobulin and human thyroid peroxidase is related to murine experimental autoimmune thyroiditis.
We have investigated functional common T-cell epitopes between human thyroglobulin (hTg) and human thyroid peroxidase (hTPO) in mice. Four hTg peptides, Tg-P1, Tg-P2, Tg-P3 and Tg-P4, in which 5 amino acid residues are identical to those of hTPO, and 1 hTPO peptide, TPO-P4 relevant to Tg-P4, were prepared. Among these peptides, only Tg-P4 (residues 2730-2743) and TPO-P4 (residues 118-131) were highly antigenic and both peptides shared the common T-cell epitope. In addition, when the spleen cells from mice immunized with mouse Tg (mTg) were restimulated in vitro by Tg-P4 or TPO-P4 as well as by mTg, these cells transferred thyroiditis to naive recipient mice. These findings indicate that this common T-cell epitope between hTg and hTPO is immunogenic and related to the development of murine experimental autoimmune thyroiditis.
Hoshioka
A
A
Department of Pediatrics, School of Medicine, Chiba University, Japan.
Kohno
Y
Y
Katsuki
T
T
Shimojo
N
N
Maruyama
N
N
Inagaki
Y
Y
Yokochi
T
T
Tarutani
O
O
Hosoya
T
T
Niimi
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D004195:Disease Models, Animal; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006801:Humans; D016219:Immunotherapy, Adoptive; D007453:Iodide Peroxidase / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013954:Thyroglobulin / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology*; D013967:Thyroiditis, Autoimmune / Q000276:immunology*
D000939:Epitopes; D010455:Peptides; D013954:Thyroglobulin; D007453:Iodide Peroxidase