title: X chromosome retains the memory of its parental origin in murine embryonic stem cells.
abstract: A cytogenetic and biochemical study of balloon-like cystic embryoid bodies, formed by newly established embryonic stem (ES) cell lines having a cytogenetically or genetically marked X chromosome, revealed that the paternally derived X chromosome was inactivated in the majority of cells in the yolk sac-like mural region consisting of the visceral endoderm and mesoderm. The nonrandomness was less evident in the more solid polar region containing the ectodermal vesicle, mesoderm and visceral endoderm. Since the same was true in embryoid bodies derived from ES cells at the 30th subculture generation, it was concluded that the imprinting responsible for the preferential inactivation of the paternal X chromosome that was limited to non-epiblast cells of the female mouse embryos, was stably maintained in undifferentiated ES cells. Differentiating epiblast cells should be able to erase or avoid responding to the imprint.
authors.affiliation: Research Center for Molecular Genetics, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tada
authors.forename: M
authors.initials: M
authors.lastname: Tada
authors.forename: N
authors.initials: N
authors.lastname: Takagi
mesh_terms: D000818:Animals; D001374:Azacitidine / Q000494:pharmacology; D002460:Cell Line; D004303:Dosage Compensation, Genetic; D005260:Female; D005821:Genetic Techniques; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology*; D014960:X Chromosome
chemical_list: D001374:Azacitidine