Isolation of a cDNA encoding the chicken p50B/p97 (Lyt-10) transcription factor.
NF-kappa B is a transcription factor composed of the p50 and p65 subunits. Recent works identified another human gene which encodes a molecule related to the p50 subunit, termed p50B, p49 or lyt-10. Here, we isolated the cDNA clones encoding chicken p50B/p97 (Lyt-10). The deduced amino acid sequence of the precursor protein, p97, shows conservation of the overall structure, and 86% identity in the Rel homology domain (RHD) and 56% identity in the ankyrin repeat domain (ARD) to human p50B/p97. Expression of this gene is highest in the chicken spleen.
Ikeda
T
T
National Institute of Animal Health, Ibaraki, Japan.
Hirota
Y
Y
Onodera
T
T
D000595:Amino Acid Sequence; D000818:Animals; D017487:Ankyrins / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D002645:Chickens / Q000235:genetics*; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000302:isolation & purification; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics*; D052003:NF-kappa B p52 Subunit; D011061:Poly A / Q000032:analysis / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000235:genetics; D020569:Proto-Oncogene Proteins c-rel; D012313:RNA / Q000032:analysis / Q000378:metabolism; D012333:RNA, Messenger; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000378:metabolism
D017487:Ankyrins; D017931:DNA Primers; D018076:DNA, Complementary; D016328:NF-kappa B; D052003:NF-kappa B p52 Subunit; D011498:Protein Precursors; D011518:Proto-Oncogene Proteins; D020569:Proto-Oncogene Proteins c-rel; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA