NMR solution structure of a peptide nucleic acid complexed with RNA.
Peptide nucleic acids (PNA) incorporating nucleic acid bases into an achiral polyamide backbone bind to DNA in a sequence-dependent manner. The structure of a PNA-ribonucleic acid (RNA) complex was determined with nuclear magnetic resonance methods. A hexameric PNA formed a 1:1 complex with a complementary RNA that is an antiparallel, right-handed double helix with Watson-Crick base pairing similar to the "A" form structure of RNA duplexes. The achiral PNA backbone assumed a distinct conformation upon binding that differed from previously proposed models and provides a basis for further structure-based design of antisense agents.
Brown
S C
SC
Glaxo Research Institute, Research Triangle Park, NC 27709.
Thomson
S A
SA
Veal
J M
JM
Davis
D G
DG
D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*
D009838:Oligodeoxyribonucleotides; D010455:Peptides; D012313:RNA