[16S rRNA reverse transcription-polymerase chain reaction of leptospires].
We amplified the leptospiral RNAs of Leptospira interrogans serovar lai strain Lai and L. biflexa serovar patoc strain Patoc I, extracted by the method of SiO2-high concentration salt solution (8 mol/L GuHCl) absorption, with the 16S rRNA gene primers of Leptospira interrogans by reverse transcription-polymerase chain reaction (RT-PCR). It demonstrated that the detecting sensitivity by naked eye after electrophoresis could be 100 times higher when we amplified DNA and RNA of leptospires at the same time by RT-PCR.
Wu
W
W
Dai
B
B
D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D007919:Leptospira / Q000235:genetics; D007921:Leptospira interrogans / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase
D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012194:RNA-Directed DNA Polymerase