title: Oligodendrocyte development and differentiation in the rumpshaker mutation.
abstract: The jimpy rumpshaker (jprsh) mutation is an amino acid substitution in exon 4 (Ile186-->Thr) of the proteolipid protein (PLP) gene on the X chromosome. Affected mice show moderate hypomyelination of the central nervous system (CNS) with increased numbers of oligodendrocytes in the white matter of the spinal cord, a feature distinguishing them from other PLP mutations such as jp, in which premature cell death occurs with reduced numbers of oligodendrocytes. Myelin sheaths of jprsh immunostain for myelin basic protein (MBP) and DM-20, but very few contain PLP. This study examines the differentiation of oligodendrocytes cultured from the spinal cords of young mutant and wild type mice using various surface and cytoplasmic antigenic markers to define the stage of development. The majority of oligodendrocytes from mutant mice progress normally to express MBP; approximately 30%, relative to wild type, contain DM-20 at the in vivo age of 16 days, but very few immunostain for PLP or the O10 and O11 markers. The morphology of mutant cells in respect to membrane sheets and processes appears similar to normal. The jprsh oligodendrocyte is, therefore, characterized by a failure to express the markers indicative of the most mature cell; however, it is probably able to achieve a normal period of survival. These data, taken in conjunction with previous results, suggest that the PLP gene has at least two functions; one, probably involving PLP, is concerned with a structural role in normal myelin compaction; the other, perhaps related to DM-20 (or another lower molecular weight proteolipid), is essential for cell survival. The mutation in jprsh at residue 186 suggests that this region, which is common to PLP and DM-20, is not critical for this latter function.
authors.affiliation: Department of Veterinary Surgery, University of Glasgow, Scotland.
authors.forename: M L
authors.initials: ML
authors.lastname: Fanarraga
authors.forename: I U
authors.initials: IU
authors.lastname: Sommer
authors.forename: I R
authors.initials: IR
authors.lastname: Griffiths
authors.forename: P
authors.initials: P
authors.lastname: Montague
authors.forename: N P
authors.initials: NP
authors.lastname: Groome
authors.forename: K A
authors.initials: KA
authors.lastname: Nave
authors.forename: A
authors.initials: A
authors.lastname: Schneider
authors.forename: P J
authors.initials: PJ
authors.lastname: Brophy
authors.forename: P G
authors.initials: PG
authors.lastname: Kennedy
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D005455:Fluorescent Antibody Technique; D007150:Immunohistochemistry; D051379:Mice; D008816:Mice, Jimpy / Q000235:genetics*; D008817:Mice, Mutant Strains; D009154:Mutation; D009185:Myelin Proteins / Q000032:analysis / Q000235:genetics*; D018991:Myelin Proteolipid Protein; D009186:Myelin Sheath / Q000378:metabolism; D009419:Nerve Tissue Proteins; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D011510:Proteolipids / Q000032:analysis; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism; D014960:X Chromosome
chemical_list: D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; C047516:Plp1 protein, mouse; D011510:Proteolipids