title: Isolation and characterization of two new N-glycosidase type-1 ribosome-inactivating proteins, unrelated in amino-acid sequence, from Petrocoptis species.
abstract: Two new N-glycosidase type-1 ribosome-inactivating proteins (RIPs), denoted petroglaucin 1 and petrograndin, respectively, were isolated from the plants Petrocoptis glaucifolia (Lag.) Boiss sp. viscosa (Rothm.) Lainz and Petrocoptis grandiflora Rothm. These new RIPs do not share H2N-terminal amino-acid sequence homology with petroglaucin (now denoted as petroglaucin 2), the only other type-1 RIP to be isolated from P. glaucifolia (Arias et al. (1992) Planta 186, 532-540). Petroglaucin 1 shares amino-acid sequence homology with RIPs from Cucurbitaceae while petroglaucin 2 and petrograndin do so with saporins and dianthin 30 (Caryophyllaceae). The new RIPs strongly inhibited protein synthesis at subnanomolar concentrations in rabbit reticulocyte lysates and other eukaryotic cell-free systems, but they were inactive on bacterial ribosomes.
authors.affiliation: Departamento de Bioquímica, Biología Molecular y Fisiología, Facultad de Ciencias, Universidad de Valladolid, Spain.
authors.forename: F J
authors.initials: FJ
authors.lastname: Arias
authors.forename: M A
authors.initials: MA
authors.lastname: Rojo
authors.forename: J M
authors.initials: JM
authors.lastname: Ferreras
authors.forename: R
authors.initials: R
authors.lastname: Iglesias
authors.forename: R
authors.initials: R
authors.lastname: Muñoz
authors.forename: F
authors.initials: F
authors.lastname: Soriano
authors.forename: E
authors.initials: E
authors.lastname: Méndez
authors.forename: L
authors.initials: L
authors.lastname: Barbieri
authors.forename: T
authors.initials: T
authors.lastname: Girbés
mesh_terms: D000595:Amino Acid Sequence; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010940:Plant Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D010944:Plants / Q000737:chemistry*; D014176:Protein Biosynthesis / Q000187:drug effects; D012313:RNA / Q000187:drug effects; D012270:Ribosomes / Q000187:drug effects*; D017386:Sequence Homology, Amino Acid
chemical_list: D010940:Plant Proteins; D012313:RNA; D006026:Glycoside Hydrolases; D009699:N-Glycosyl Hydrolases; C091231:petroglaucin 1 protein, Petrocoptis glaucifolia; C091232:petrograndin