Partial cDNA cloning and NGF regulation of a rat 5-HT3 receptor subunit.
Nerve growth factor (NGF) stimulates 5-HT3 receptor expression although the mechanism(s) responsible for this effect are unknown. We report the nucleotide and deduced amino acid sequences of a nearly complete rat 5-HT3 receptor subunit and use the sequence to develop a reverse transcription-polymerase chain reaction (RT-PCR) assay that measures 5-HT3 mRNA. Exposure of PC12 cells to NGF results in increasing steady-state levels of 5-HT3 mRNA. The four-fold increase in mRNA correlates with and is sufficient to account for increases in receptor measured by agonist induction of whole cell currents.
Isenberg
K E
KE
Department of Psychiatry, Washington University School of Medicine, St Louis, MO 63110.
Ukhun
I A
IA
Holstad
S G
SG
Jafri
S
S
Uchida
U
U
Zorumski
C F
CF
Yang
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D016716:PC12 Cells / Q000187:drug effects / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D011985:Receptors, Serotonin / Q000096:biosynthesis / Q000235:genetics*; D017783:Superior Cervical Ganglion / Q000737:chemistry; D015854:Up-Regulation / Q000187:drug effects
D018076:DNA, Complementary; D009363:Neoplasm Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011985:Receptors, Serotonin; D012194:RNA-Directed DNA Polymerase