Activation of macrophages for cytolysis of virally infected cells by monoclonal antibody to the 73-kDa lipopolysaccharide receptor.
The abilities of lipopolysaccharide (LPS) and mAb5D3 (a monoclonal antibody specific for the 73 kDa LPS receptor) to activate bone marrow culture derived macrophages (BMCM) for cytolysis of vesicular stomatitis virus infected BALB/c3T3 (VSV-3T3) and the tumor target, P815, were compared. Activation for cytolysis of VSV-3T3 cells occurred at a lower level of either LPS or mAb5D3 than activation for cytolysis of P815. With both targets and under several stimulation conditions there was a constant differential between the two activating stimuli. These data indicate that stimulation of the 73 kDa LPS receptor at low levels of either mAb5D3 or LPS can activate BMCM for cytolysis of VSV-3T3 but not P815. Further, the constant relative efficiency of mAb5D3 to LPS in both target systems suggests that there is a common receptor for both LPS mediated effects.
LeBlanc
P A
PA
Department of Biological Sciences, University of Alabama, Tuscaloosa 35487-0334.
D016475:3T3 Cells; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011971:Receptors, Immunologic / Q000276:immunology*; D014721:Vesicular stomatitis Indiana virus
D000911:Antibodies, Monoclonal; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D011971:Receptors, Immunologic