Synergism between platelet activating factor and C-C chemokines for arachidonate release in human monocytes.
Monocyte Chemotactic Protein(MCP)-1 and other members of the C-C branch of the chemokine superfamily (RANTES, MIP1 alpha/LD78, and MCP-3) induced, at chemotactic concentrations, the release of [3H]arachidonic acid in prelabeled human monocytes. Arachidonate release was potentiated (2 to 4 fold) in the presence of platelet activating factor (PAF). PAF effect was blocked by a specific receptor antagonist, WEB 2187, and was not present when inactive analogs, PAF inactive enantiomer, or lysoPAF were used. Thus, the synergistic action of PAF with C-C chemokines, in terms of arachidonate release and chemotaxis, is specific for this alkylphospholipid and is receptor mediated. Reciprocal potentiation of PAF and C-C chemokines may be important at sites of inflammation.
Sozzani
S
S
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Rieppi
M
M
Locati
M
M
Zhou
D
D
Bussolino
F
F
Proost
P
P
Van Damme
J
J
Mantovani
A
A
D016718:Arachidonic Acid / Q000097:blood*; D018932:Chemokine CCL2; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D054410:Chemokine CCL7; D002630:Chemotactic Factors / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D016207:Cytokines / Q000494:pharmacology*; D004357:Drug Synergism; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008222:Lymphokines / Q000494:pharmacology; D019402:Macrophage Inflammatory Proteins; D018945:Monocyte Chemoattractant Proteins; D015846:Monokines / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000494:pharmacology*
C515450:CCL7 protein, human; D018932:Chemokine CCL2; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D054410:Chemokine CCL7; D002630:Chemotactic Factors; D016207:Cytokines; D008222:Lymphokines; D019402:Macrophage Inflammatory Proteins; D018945:Monocyte Chemoattractant Proteins; D015846:Monokines; D010972:Platelet Activating Factor; D016718:Arachidonic Acid