title: Antigen-presenting capacity in normal human dermis is mainly subserved by CD1a+ cells.
abstract: A proposed role for antigen-presenting dermal dendrocytes in the pathogenesis of many dermal inflammatory skin diseases remains speculative. We therefore sought to determine the phenotype and functional characteristics of antigen-presenting cells isolated from normal human dermis. Normal adult human skin was incubated overnight with dispase at 4 degrees C, the epidermis was removed, and the residual dermal preparation was then minced and digested with a mixture of hyaluronidase, collagenase, and DNAase at 37 degrees C, prior to filtration through mesh. Dermal cell suspensions thus obtained were stained using specific monoclonal antibodies, and analysed by fluorescence microscopy or flow cytometry. Mean values were as follows: CD45+ leucocytes 39%, HLA-DR+ cells 39%, Ulex europaeus agglutinin I+ endothelial cells 26%, CD1a+ cells 3.9%, CD11b+ cells 16%, CD11c+ cells 6%. Mitomycin C-treated crude dermal cell suspensions induced allostimulation of peripheral blood mononuclear cells in a 7-day culture, as assessed by 3H-TdR incorporation. Depletion of CD1a+ Langerhans-like cells from the dermal cell preparation, by 95, 74 and 90% in three separate experiments using immunomagnetic beads, reduced 3H-TdR incorporation at optimal responder-to-stimulator cell ratios by 90, 64, and 87%, respectively. Our findings suggest that, in normal human dermis, the great majority of the alloantigen-presenting capacity resides in the CD1a+ Langerhans cell-like dendritic antigen-presenting cell population, and not to any great extent in either CD1a- macrophage-like cells, or HLA-DR+ endothelial cells. The relationship of the CD1a+ dermal antigen-presenting cells to the Langerhans cell lineage remains to be determined.
authors.affiliation: St John's Institute of Dermatology, London, U.K.
authors.forename: C
authors.initials: C
authors.lastname: Sepulveda-Merrill
authors.forename: S
authors.initials: S
authors.lastname: Mayall
authors.forename: A S
authors.initials: AS
authors.lastname: Hamblin
authors.forename: S M
authors.initials: SM
authors.lastname: Breathnach
mesh_terms: D000328:Adult; D017951:Antigen Presentation / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D018949:Antigens, CD1; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D004727:Endothelium / Q000276:immunology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D007202:Indicators and Reagents; D007801:Langerhans Cells / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000276:immunology
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D006684:HLA-DR Antigens; D007202:Indicators and Reagents