Mutagenicity of heterocyclic amines when activated by pancreas tissue.
The heterocyclic amines (HA) 2-aminodipyrido[1,2-a:3',2-d]imidazole (Glu-P-2), 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) were mutagenic in V79 cells (Chinese hamster lung fibroblasts) using 6-thioguanine resistance as the marker of mutagenicity. Pancreas duct epithelial cells (DEC) from untreated hamsters, homogenates of pancreas ducts from untreated hamsters and those fed a high fat diet and human DEC were used to activate the heterocyclic amines. When hamster cells and tissues were used the optimum mutation frequencies (mutants/10(6) survivors) measured were: Glu-P-2, 10 +/- 1; MeIQ, 28 +/- 2 (DEC), 12 +/- 2 (control, duct homogenate), and 21 +/- 2 (high fat diet fed, duct homogenate); PhIP, 61 +/- 5. When human DEC were used the optimum mutation frequencies were: MeIQ, 32 +/- 4; PhIP, 35 +/- 3. 3,8-Dimethylimidazo[4,5-f]quinoxaline, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole and 3-amino-1-methyl-5H-pyrido[4,3-b]indole were not mutagenic in this assay.
Lawson
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Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68198-6805.
Kolar
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D000293:Adolescent; D000328:Adult; D000588:Amines / Q000494:pharmacology*; D000818:Animals; D002243:Carbolines / Q000494:pharmacology; D002478:Cells, Cultured; D006224:Cricetinae; D006571:Heterocyclic Compounds / Q000494:pharmacology*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D008168:Lung / Q000166:cytology; D008297:Male; D009153:Mutagens / Q000494:pharmacology*; D010183:Pancreatic Ducts / Q000166:cytology / Q000187:drug effects*; D011804:Quinolines / Q000494:pharmacology
D000588:Amines; D002243:Carbolines; D006571:Heterocyclic Compounds; D007093:Imidazoles; D009153:Mutagens; D011804:Quinolines; C019133:3-amino-1-methyl-5H-pyrido(4,3-b)indole; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine; C036989:2-amino-3,4-dimethylimidazo(4,5-f)quinoline; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole; C023847:Glu-P-2