Detection of microsporidial spores in fecal specimens from patients diagnosed with cryptosporidiosis.
Patients infected with Cryptosporidium parvum may have concurrent infections with microsporidia. Two modified trichrome stains and a polyclonal indirect fluorescent-antibody procedure were used for the detection of microsporidia; the Merifluor Cryptosporidium-Giardia monoclonal direct immunofluorescence detection kit was used for the detection of C. parvum. Formalinized stool specimens from 60 immunocompromised patients strongly suspected of having or previously diagnosed with cryptosporidiosis or microsporidiosis were examined. All patients were positive for one or both parasites, 18 (30%) with C. parvum only, 25 (42%) with microsporidia only, and 17 (28%) with both C. parvum and microsporidia. These findings emphasize the importance of considering both organisms as potential causative agents of diarrhea in compromised patients.
Garcia
L S
LS
Department of Pathology, UCLA Medical Center 90024-1713.
Shimizu
R Y
RY
Bruckner
D A
DA
D000818:Animals; D001391:Azo Compounds; D003457:Cryptosporidiosis / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D004801:Eosine Yellowish-(YS); D005243:Feces / Q000469:parasitology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008739:Methyl Green; D016816:Microsporida / Q000302:isolation & purification*; D013170:Spores / Q000302:isolation & purification
D001391:Azo Compounds; C017768:trichrome stain; D008739:Methyl Green; D004801:Eosine Yellowish-(YS)