NusA changes the conformation of Escherichia coli RNA polymerase at the binding site for the 3' end of the nascent RNA.
A conformational change in Escherichia coli RNA polymerase induced by NusA was detected by utilizing photocrosslinking. A change in the binding site for the 3' end of the RNA occurred, and NusA increased interactions of the RNA with the beta subunit of the polymerase. NusA was not contacted by the 3' end of the RNA.
Zhang
Y
Y
Department of Botany-Microbiology, University of Oklahoma, Norman 73019.
Hanna
M M
MM
D001426:Bacterial Proteins / Q000502:physiology*; D001483:Base Sequence; D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D010445:Peptide Elongation Factors; D011487:Protein Conformation; D012329:RNA, Bacterial / Q000737:chemistry* / Q000378:metabolism; D014157:Transcription Factors / Q000502:physiology*; D035602:Transcriptional Elongation Factors
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D010445:Peptide Elongation Factors; D012329:RNA, Bacterial; D014157:Transcription Factors; D035602:Transcriptional Elongation Factors; C068727:nusA protein, E coli; D012321:DNA-Directed RNA Polymerases