Glutamate, GABA, calbindin-D28k and parvalbumin immunoreactivity in the pulvinar-lateralis posterior complex of the cat: relation to the projection to the Clare-Bishop area.
Neurons of the pulvinar-lateralis posterior complex (Pul-LP) containing glutamate (Glu) and GABA, as presumed neurotransmitters, and calbindin- D28k (calbindin) and parvalbumin (PV), as Ca-binding proteins, were identified in the cat by using immunohistochemical methods. In vibratome sections, neurons immunoreactive (IR) to each of the four antibodies were observed throughout the Pul-LP. In semithin sections, GABA-IR neurons were also PV-IR but not calbindin-IR and some of them also co-localized Glu. The Glu-IR neurons which were negative for GABA co-localized calbindin but not PV. The neurons of the Pul-LP projecting to the Clare-Bishop area (CB) in the suprasylvian gyrus were identified with a retrogradely transported tracer and the sections were then immunostained for Glu, GABA, calbindin and PV. Only Glu- and calbindin-IR neurons were retrogradely labeled. These results show that, if calbindin and PV have a Ca-binding role, the presumably excitatory Glu-IR neurons projecting to the CB are use calbindin whereas the presumably inhibitory GABA-IR neurons are intrinsic and use PV. This relationship implies that these proteins probably have other roles specifically related to the kind of agonist to be released at the neuron.
Palestini
M
M
Departamento de Ciencias Preclinicas, Facultad de Medicina, Universidad de Chile, Santiago.
Guegan
M
M
Saavedra
H
H
Thomasset
M
M
Batini
C
C
D000818:Animals; D064026:Calbindins; D002415:Cats; D002540:Cerebral Cortex / Q000166:cytology / Q000378:metabolism*; D005971:Glutamates / Q000276:immunology / Q000378:metabolism; D018698:Glutamic Acid; D017979:Gold Colloid; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D009411:Nerve Endings / Q000276:immunology / Q000378:metabolism; D009412:Nerve Fibers / Q000276:immunology / Q000378:metabolism; D009434:Neural Pathways / Q000166:cytology; D010320:Parvalbumins / Q000276:immunology / Q000378:metabolism; D064030:S100 Calcium Binding Protein G / Q000276:immunology / Q000378:metabolism; D013787:Thalamic Nuclei / Q000166:cytology / Q000378:metabolism*; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; D005680:gamma-Aminobutyric Acid / Q000276:immunology / Q000378:metabolism
D064026:Calbindins; D005971:Glutamates; D017979:Gold Colloid; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D006735:Horseradish Peroxidase