Modulation of protectin (CD59 antigen) cell surface expression on human neoplastic cell lines.
The ability of cytokines (IFN alpha, IFN gamma, TNF alpha, IL-1 alpha, IL-6), all-trans retinoic acid, 1,25(OH)2-vitamin D3 and the tumor promoting phorbol ester TPA to regulate cell surface expression of protectin (CD59 antigen) on human hematopoietic and non-hematopoietic neoplastic cell lines was examined with the aid of immunocytofluorometric measurements. The tumor promoting phorbol ester TPA induced a marked up-regulation of protectin in all examined cell lines with the exception of promyelocytic leukemia HL-60, where TPA significantly decreased protectin cell surface expression. All-trans retinoic acid weakly down-regulated cell surface protectin on K-562, while 1,25(OH)2-vitamin D3 produced such effect on HL-60 cells. None of the examined cytokines induced a significant protectin down-regulation in the examined cell lines.
Sedlák
J
J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Hunáková
L
L
Duraj
J
J
Grófová
M
M
Chorváth
B
B
D015703:Antigens, CD / Q000096:biosynthesis*; D001943:Breast Neoplasms / Q000276:immunology / Q000473:pathology; D018957:CD59 Antigens; D002117:Calcitriol / Q000494:pharmacology; D002277:Carcinoma / Q000276:immunology* / Q000473:pathology; D002462:Cell Membrane / Q000276:immunology; D016207:Cytokines / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007951:Leukemia, Myeloid / Q000276:immunology* / Q000473:pathology; D015473:Leukemia, Promyelocytic, Acute / Q000276:immunology* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D010051:Ovarian Neoplasms / Q000276:immunology / Q000473:pathology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects
D015703:Antigens, CD; D018957:CD59 Antigens; D016207:Cytokines; D008562:Membrane Glycoproteins; D014212:Tretinoin; D002117:Calcitriol; D013755:Tetradecanoylphorbol Acetate