title: The distribution of hypothalamic nitric oxide synthase mRNA in relation to gonadotrophin-releasing hormone neurons.
abstract: Using probes for rat neural nitric oxide synthase (NOS) mRNA and GnRH mRNA, we performed in situ hybridization to survey NOS mRNA distribution within the hypothalamus of the male and female rat and sought evidence for its expression in GnRH neurons. The NOS cRNA probe was radiolabelled with 35S, and a digoxigenin-labeled rat GnRH cRNA probe was used for double-label studies. NOS mRNA was localized in discrete hypothalamic areas, in grain clusters suggestive of individual neurons. NOS mRNA-positive cells were located mainly in the supraoptic and paraventricular nucleus, particularly overlying the magnocellular division. Rostrally, cells expressing NOS mRNA were especially prominent in the diagonal band of Broca, in a distribution very similar to GnRH neurons. Nevertheless, only one of 370 cells labeled for GnRH mRNA appeared to be positive for NOS mRNA. We conclude that NOS mRNA is located prominently in regions where CRH, AVP and oxytocin cells are located. NOS mRNA-positive cells are located in close proximity to GnRH neurons, but rarely do such neurons express NOS mRNA.
authors.affiliation: Department of Endocrinology, St. Bartholomew's Hospital, London, UK.
authors.forename: A B
authors.initials: AB
authors.lastname: Grossman
authors.forename: W G
authors.initials: WG
authors.lastname: Rossmanith
authors.forename: E B
authors.initials: EB
authors.lastname: Kabigting
authors.forename: G
authors.initials: G
authors.lastname: Cadd
authors.forename: D
authors.initials: D
authors.lastname: Clifton
authors.forename: R A
authors.initials: RA
authors.lastname: Steiner
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000235:genetics*; D000818:Animals; D015342:DNA Probes; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D009474:Neurons / Q000378:metabolism*; D019001:Nitric Oxide Synthase; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D007987:Gonadotropin-Releasing Hormone; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases