Structural studies of the O-antigenic polysaccharides of Escherichia coli O3 and the enteroaggregative Escherichia coli strain 17-2.
The polysaccharide part of the lipopolysaccharide isolated from an enteroaggregative Escherichia coli isolated from a young child with diarrhoea in Santiago, Chile (strain 17-2), has been investigated. Sugar and methylation analyses of native and partially degraded polysaccharide together with 1H-NMR and 13C-NMR spectroscopies revealed that the polysaccharide is composed of pentasaccharide repeating units. The structure of the repeating unit of E. coli strain 17-2 O-polysaccharide is: [formula: see text] The structure of the O-polysaccharide from E. coli O3 was shown to be identical to that of E. coli strain 17-2 by sugar and methylation analyses and by 1H-NMR and 13C-NMR spectroscopies.
Medina
E C
EC
Department of Immunology, Microbiology, Pathology and Infectious Diseases, Karolinska Institute, Sweden.
Widmalm
G
G
Weintraub
A
A
Vial
P A
PA
Levine
M M
MM
Lindberg
A A
AA
D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry*
D019081:O Antigens; D011135:Polysaccharides, Bacterial