title: Functional conservation of the neutralizing domains on the external envelope glycoprotein of cosmopolitan and melanesian strains of human T cell leukemia/lymphoma virus type I.
abstract: To determine if the immunodominant neutralizing epitopes on the external envelope glycoprotein of the recently identified sequence variants of human T cell leukemia/lymphoma virus type I (HTLV-I) from Melanesia are functionally conserved, sera from Japanese patients with adult T cell leukemia and from HTLV-I-infected Melanesians of Papua New Guinea and the Solomon Islands were tested for neutralizing antibodies by use of vesicular stomatitis virus pseudotypes bearing envelope antigens of Japanese and Melanesian HTLV-I strains. Neutralizing antibody titers of the Japanese and Melanesian sera and of monoclonal and polyclonal antibodies directed against a known neutralizing epitope on the external envelope glycoprotein of HTLV-I were equivalent against the Japanese and Melanesian HTLV-I pseudotypes. The demonstrated two-way cross-neutralization between Japanese and Melanesian strains of HTLV-I indicates that their antigenic determinants for neutralization are functionally indistinguishable and that HTLV-I exists as a single serotype worldwide.
authors.affiliation: Department of Hygiene and Virology, Gunma University School of Medicine, Maebashi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hoshino
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: I
authors.initials: I
authors.lastname: Miyoshi
authors.forename: R
authors.initials: R
authors.lastname: Yanagihara
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002415:Cats; D002460:Cell Line; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D015686:Gene Products, env / Q000276:immunology*; D015486:HTLV-I Antigens / Q000276:immunology*; D015368:Human T-lymphotropic virus 1 / Q000145:classification / Q000276:immunology*; D006801:Humans; D007564:Japan; D008542:Melanesia; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction; D015682:Retroviridae Proteins, Oncogenic / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology
chemical_list: D000939:Epitopes; D015686:Gene Products, env; D015486:HTLV-I Antigens; D015682:Retroviridae Proteins, Oncogenic; C055266:gp46 protein, Human T-cell leukemia virus type I