Mapping the bovine homolog of the human cystic fibrosis gene.
Human cDNA probe H1.6 (clone 10-1) encoding cystic fibrosis transmembrane conductance regulator (CFTR) was used to map the bovine homolog of CFTR. Using a panel of bovine x rodent hybrid somatic cells, the homolog was mapped to bovine syntenic group U13 which corresponds to chromosome 4. CFTR is 97.7% concordant with syntenic markers T-cell receptor beta (TCRB) and P-glycoprotein 3 (PGY3). The comparative gene maps of CFTR, TCRB, and PGY3 on human chromosome 7, bovine chromosome 4, and mouse chromosome 5 and 6 indicate conservation of synteny, although internal rearrangements relative to HSA 7 are present in both cattle and mice.
Wallis
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Texas A&M University, College Station 77843.
Womack
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JE
D000818:Animals; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000662:veterinary*; D003550:Cystic Fibrosis / Q000235:genetics / Q000662:veterinary*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D015342:DNA Probes; D018076:DNA, Complementary; D006801:Humans; D006822:Hybrid Cells; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice
C505032:CFTR protein, human; D015342:DNA Probes; D018076:DNA, Complementary; D008565:Membrane Proteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator