2-Chloro-2'-deoxyadenosine (2-CdA) combined with cyclosporine A successfully prevents rejection of fetal brain stem allograft in rabbits.
Allografts of brain stem from 20-day-old fetuses to nucleus caudatus of adult rabbits were performed. To prevent graft rejection immunosuppression with 2-CdA and cyclosporine A was transiently induced. Graft survival were assessed by histological and electrophysiological techniques. Both morphological (synaptogenesis, myelinization) and functional (generation of rhythmic neuronal activity) signs of graft maturation were found after nine weeks. The data suggest that transient immunosuppression used is sufficient to induce tolerance to neural graft, and no interference with maturation of implanted fetal tissue occurs.
Grieb
P
P
Department of Neurophysiology, Polish Academy of Sciences, Warsaw.
Ryba
M
M
Janczewski
W
W
Sawicki
J
J
Jagielski
J
J
Andrychowski
J
J
D000818:Animals; D001933:Brain Stem / Q000196:embryology / Q000502:physiology / Q000637:transplantation*; D016380:Brain Tissue Transplantation / Q000276:immunology*; D017338:Cladribine / Q000627:therapeutic use*; D016572:Cyclosporine / Q000627:therapeutic use*; D004594:Electrophysiology; D005260:Female; D016332:Fetal Tissue Transplantation / Q000276:immunology*; D006084:Graft Rejection / Q000517:prevention & control*; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D008297:Male; D011247:Pregnancy; D011817:Rabbits
D007166:Immunosuppressive Agents; D017338:Cladribine; D016572:Cyclosporine