Construction of a new teichuronopeptide-defective derivative from alkaliphilic Bacillus sp. C-125 by cell fusion.
Cell walls of facultative alkaliphilic Bacillus sp. C-125 are composed of peptidoglycan, teichuronic acid, and teichuronopeptide. A mutant lacking teichuronic acid, or defective in both teichuronic acid and teichuronopeptide has been isolated from the organism. We now constructed another type of a cell-wall defective mutant that was defective in teichuronopeptide but had teichuronic acid, by cell fusion using protoplasts prepared from a wild-type strain and a mutant defective in teichuronopeptide and teichuronic acid. This mutant grew more poorly than wild type or a teichuronic acid-defective strain of C-125. The growth, however, was faster than that of the parental strain defective in both teichuronic acid and teichuronopeptide.
Ito
M
M
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Tabata
K
K
Aono
R
R
D001407:Bacillus / Q000254:growth & development / Q000502:physiology*; D005965:Glucuronates / Q000737:chemistry*; D009154:Mutation; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D011523:Protoplasts / Q000502:physiology
D005965:Glucuronates; C065664:teichuronopeptide; D011099:Polyglutamic Acid