[Analysis of membrane expression of the CD63 human basophil activation marker. Applications to allergologic diagnosis].
On the basis of the CD 63 bi-modal expression on the membrane of activated basophils, we set up a flow cytometric method for the analysis of human basophils activation by an anti-IgE and anti-CD 63 double labelling. We demonstrated that the statistical characteristics of the percentages of activation obtained by an anti-IgE stimulation allowed the use of this method for pharmacological studies. The percentages of activation were of the same order of magnitude than those obtained by histamine release. CD 63 expression was also observed for a low affinity allergen such as the sulfonyl-HSA conjugate used for sulfites hypersensibility diagnosis, healthy donors being negative. This method, which can be automatized may represent an interesting candidate in the field of hapten hypersensitivity which lacks of reliable diagnostical methods.
Sainte-Laudy
J
J
Département des maladies respiratoires, Hôpital de la Croix-Rousse, Lyon.
Vallon
C
C
Guérin
J C
JC
D000423:Alcian Blue; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D015549:Basophil Degranulation Test / Q000379:methods*; D001491:Basophils / Q000276:immunology*; D005434:Flow Cytometry / Q000379:methods*; D006636:Histamine Release; D006801:Humans; D010980:Platelet Membrane Glycoproteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000276:immunology; D000075462:Serum Albumin, Human; D013194:Staining and Labeling; D060149:Tetraspanin 30
D000888:Antibodies, Anti-Idiotypic; D015703:Antigens, CD; D000954:Antigens, Surface; C554586:CD63 protein, human; D010980:Platelet Membrane Glycoproteins; D012709:Serum Albumin; D060149:Tetraspanin 30; C026408:anti-IgE antibodies; C088342:sulfonyl-human serum albumin; D000423:Alcian Blue; D000075462:Serum Albumin, Human