Micronuclei and mitotic index in B-, T4- and T8-cells treated with mitomycin C and gamma-irradiation.
Lymphocytes were treated in vitro with mitomycin C and gamma-radiation at different doses (0-250 nmol/l and 0-2 Gy, respectively). After incubation in RPMI 1640 medium and stimulation with phytohemagglutinin for 72 h, the lymphocyte subgroups T4 (CD4), T8 (CD8) and B (CD19) were separated by an immunomagnetic method and analyzed for the presence of micronuclei. With mitomycin C the highest levels were found in T4- and B-cells. When micronuclei were induced by irradiation the T4-cells showed the highest frequencies and the B-cells the lowest. The outcome of B-cells with gamma-irradiation was probably due to a pronounced cytotoxic reaction in this cell type, which could be measured as a decrease in mitotic index.
Holmén
A
A
Department of Occupational Medicine, Central Hospital, Halmstad, Sweden.
Karlsson
A
A
Bratt
I
I
Högstedt
B
B
D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D005720:Gamma Rays; D006801:Humans; D016131:Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D015162:Micronucleus Tests; D016685:Mitomycin / Q000494:pharmacology*; D008940:Mitotic Index / Q000187:drug effects / Q000528:radiation effects; D009154:Mutation; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects
D010835:Phytohemagglutinins; D016685:Mitomycin