Total parenteral nutrition decreases luminal mucous gel and increases permeability of small intestine.
The distribution of fluorescein isothiocyanate dextran 70,000 (FITC-dextran) and mucous gel across the lumen of small intestine was observed as an investigation into the role of mucous gel on permeability in total parenteral nutrition (TPN). Thirty-two rats were randomly divided into two groups fed with either TPN or oral rat food. On day 4 or 7, FITC-dextran (750 mg/kg body weight) was given through the gastroduodenal tube. After 1 hour, blood samples were taken by aortic puncture to analyze plasma FITC-dextran by fluorescence spectrometry. Samples of small intestine with luminal contents were frozen and sectioned in a cryostat for fluorescence microscopy; the same sections were placed in a 0.2% celloidin solution for 3 minutes to preserve mucous gel and stained by periodic acid-Schiff reaction for light microscopy. The plasma level of FITC-dextran after 1 hour of this marker injection showed a significant increase (p < .01) in the TPN group compared with the rat food group on days 4 and 7. Morphologic findings on days 4 and 7 were similar in both the jejunum and ileum: The mucous gel filled the spaces between villi and FITC-dextran centered in the lumen in the rat food group, whereas the mucous gel decreased and FITC-dextran filled the spaces between villi in the TPN group. FITC-dextran and mucous gel showed complementary distributions in both groups. These data suggest that TPN decreases luminal mucous gel and increases permeability of small intestine in rats.
Iiboshi
Y
Y
Department of Pediatric Surgery, Osaka University Medical School, Japan.
Nezu
R
R
Kennedy
M
M
Fujii
M
M
Wasa
M
M
Fukuzawa
M
M
Kamata
S
S
Takagi
Y
Y
Okada
A
A
D000818:Animals; D003911:Dextrans; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D007082:Ileum / Q000033:anatomy & histology; D007413:Intestinal Mucosa / Q000378:metabolism*; D007421:Intestine, Small / Q000033:anatomy & histology / Q000378:metabolism*; D007583:Jejunum / Q000033:anatomy & histology; D008297:Male; D008856:Microscopy, Fluorescence; D009093:Mucus / Q000502:physiology*; D010289:Parenteral Nutrition, Total / Q000009:adverse effects*; D010539:Permeability; D051381:Rats; D017207:Rats, Sprague-Dawley; D013050:Spectrometry, Fluorescence
D003911:Dextrans; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate