title: Interactions of atrial natriuretic peptide with A7r5 cells.
abstract: The interactions of Atrial Natriuretic Peptide with its receptors were investigated with the vascular smooth muscle cells from the A7r5 cell line. Displacement experiments of [125I]rat-Atrial Natriuretic Peptide by rat-Atrial Natriuretic Peptide revealed a single class of receptors with a Kd = 0.21 +/- 0.08 nM and a Bmax = of 35 +/- 16 fmol/mg of protein. Furthermore, the complex [125I]r-Atrial Natriuretic Peptide was internalised by a heatsensitive process. Finally, Atrial Natriuretic Peptide increased cyclic Guanosyl 3',5' Mono Phosphate in a time dependent and dose dependent way, a concentration of 0.1 microM increasing cyclic Guanosyl 3',5' Mono Phosphate level by a factor of 8.3 times when compared to basal level.
authors.affiliation: Sheffield University, Department of Animal and Plant Sciences, Western Bank, England.
authors.forename: H B
authors.initials: HB
authors.lastname: Aptel
authors.forename: C F
authors.initials: CF
authors.lastname: Deacon
authors.forename: I W
authors.initials: IW
authors.lastname: Henderson
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism* / Q000494:pharmacology*; D001414:Bacitracin / Q000494:pharmacology; D001667:Binding, Competitive; D002460:Cell Line; D006152:Cyclic GMP / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D051381:Rats; D017461:Receptors, Atrial Natriuretic Factor / Q000378:metabolism*; D013997:Time Factors
chemical_list: D007457:Iodine Radioisotopes; D001414:Bacitracin; D009320:Atrial Natriuretic Factor; D017461:Receptors, Atrial Natriuretic Factor; D006152:Cyclic GMP; D015056:1-Methyl-3-isobutylxanthine