title: Localization of perforin in viral vesicles and erythema multiforme.
abstract: BACKGROUND
  Perforin (Pf), a pore-forming protein, is a cytolytic protein of killer cells. Its deposition in lesioned skin has not been studied.
  
  
  OBJECTIVE
  The purpose of this study is to show Pf deposition in the lesioned skin and Pf expression in the dermal infiltrates of various inflammatory skin diseases.
  
  
  METHODS
  Frozen specimens obtained from 29 patients with 5 different diseases were immunohistochemically stained.
  
  
  RESULTS
  Granular deposition of Pf was found in the lesioned skin in 2 out of 5 cases of viral vesicles and in 3 out of 7 cases of erythema multiforme. In the cases with Pf deposition, the percentages of Pf+ cells in the dermis were higher than in those without deposition.
  
  
  CONCLUSIONS
  Pf released from natural killer cells or cytotoxic T lymphocytes may play a role in tissue damage.
authors.affiliation: Department of Dermatology, Ehime University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sayama
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: M
authors.initials: M
authors.lastname: Tohyama
authors.forename: Y
authors.initials: Y
authors.lastname: Miki
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016516:CD4-CD8 Ratio; D002648:Child; D003877:Dermatitis, Contact / Q000378:metabolism / Q000473:pathology; D004892:Erythema Multiforme / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006562:Herpes Zoster / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007694:Killer Cells, Natural / Q000737:chemistry / Q000473:pathology; D017512:Lichenoid Eruptions / Q000378:metabolism / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis*; D008875:Middle Aged; D010391:Pemphigoid, Bullous / Q000378:metabolism / Q000473:pathology; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D012867:Skin / Q000737:chemistry / Q000473:pathology; D017193:Skin Diseases, Viral / Q000378:metabolism / Q000473:pathology*; D013194:Staining and Labeling; D013602:T-Lymphocytes, Cytotoxic / Q000737:chemistry / Q000473:pathology*
chemical_list: D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D054353:Perforin