Dissolve and capture: a strategy for analysing mRNA in blood.
Messenger RNA (mRNA) sequences were amplified from whole blood prepared in guanidine thiocyanate (GuSCN) after capture of the mRNA with an affinity membrane.
Gillespie
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Department of Neoplastic Diseases, Hahnemann University, Philadelphia, Pennsylvania 19102-1192.
Cuddy
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Kolbe
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D006146:Guanidines; D006801:Humans; D008567:Membranes, Artificial; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000097:blood*; D012194:RNA-Directed DNA Polymerase; D012260:Ribonucleases / Q000037:antagonists & inhibitors; D013861:Thiocyanates
D006146:Guanidines; D008567:Membranes, Artificial; D012333:RNA, Messenger; D013861:Thiocyanates; C054436:guanidine thiocyanate; D012194:RNA-Directed DNA Polymerase; D012260:Ribonucleases