RNA expression of complement regulatory proteins in human brain tumors.
The expression of complement regulatory proteins (CRP) on the surface of neoplastic cells has been proposed as a mechanism by which these cells evade immune surveillance. We have examined the RNA expression of the genes that encode 5 kinds of CRP in various human brain tumors to determine whether CRP expression might play a role in the malignant progression of these tumors. The benign and atypical meningiomas, and the astrocytomas showed high expression of SP-40,40, low expression of CD59, and barely detectable expression of CD46, CD55 and S-protein. The benign and atypical menigiomas showed significantly greater expression of SP-40,40 at the RNA level when compared to malignant meningiomas. This study describes the mRNA expression of meningiomas, astrocytomas, tumor cell lines and normal human tissues.
Shinoura
N
N
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, OH 45267-0524.
Heffelfinger
S C
SC
Miller
M
M
Shamraj
O I
OI
Miura
N H
NH
Larson
J J
JJ
DeTribolet
N
N
Warnick
R E
RE
Tew
J J
JJ
Menon
A G
AG
D015703:Antigens, CD / Q000235:genetics; D001254:Astrocytoma / Q000235:genetics; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics*; D018958:CD55 Antigens; D018957:CD59 Antigens; D051152:Clusterin; D003169:Complement Inactivator Proteins / Q000235:genetics*; D006023:Glycoproteins; D006801:Humans; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins / Q000235:genetics; D008579:Meningioma / Q000235:genetics; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis*; D014407:Tumor Cells, Cultured; D019096:Vitronectin
D015703:Antigens, CD; D018958:CD55 Antigens; D018957:CD59 Antigens; D051152:Clusterin; D003169:Complement Inactivator Proteins; D006023:Glycoproteins; D051916:Membrane Cofactor Protein; D008562:Membrane Glycoproteins; D018832:Molecular Chaperones; D012313:RNA; D019096:Vitronectin; C497857:CD46 protein, human; C495242:CLU protein, human