Standardization of HeLa cells preparations as the source of snRNA's for preliminary study on the effectors of pre-mRNA splicing.
Physical, chemical and biological parameters of HeLa cell cultures, which ensure obtaining standard nuclear material to be used as a source of snRNP and snRNA, have been determined. The secondary structure of U1 snRNA as the result of the conformational studies performed using S1 nuclease, A and T1 ribonuclease and Pb(II) ions, and reproducibility of the results obtained after 6-48 h exposure of the cells to actinomycin D at high concentration permit to accept the proposed method of standardization as satisfactory for investigation into snRNP and snRNA.
Hładoń
B
B
Department of Pharmacology, Medical Academy, Poznań, Poland.
Krajewski
J
J
Sloderbach
A
A
Radosh
P
P
D001483:Base Sequence; D002455:Cell Division / Q000502:physiology; D006367:HeLa Cells / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012326:RNA Splicing; D012333:RNA, Messenger / Q000502:physiology*; D012342:RNA, Small Nuclear / Q000096:biosynthesis / Q000302:isolation & purification* / Q000502:physiology
D012333:RNA, Messenger; D012342:RNA, Small Nuclear