The anti-oxidant activity of turmeric (Curcuma longa).
The turmeric anti-oxidant protein (TAP) had been isolated from the aqueous extract of turmeric. The anti-oxidant principle was found to be a heat stable protein. Trypsin treatment abolished the anti-oxidant activity. The anti-oxidant principle had an absorbance maximum at 280 nm. After gel filtration, the protein showed a 2-fold increase in anti-oxidant activity and showed 2 bands in the SDS-PAGE with approximate molecular weight range of 24,000 Da. The protein showed a concentration-dependent inhibitory effect on the promoter induced lipid peroxidation. A 50% inhibitory activity of lipid peroxidation was observed at a protein concentration of 50 micrograms/ml. Ca(2+)-ATPase of rat brain homogenate was protected to nearly 50% of the initial activity from the lipid peroxidant induced inactivation by this protein. This protection of Ca(2+)-ATPase activity was found to be associated with the prevention of loss of -SH groups.
Selvam
R
R
Department of Medical Biochemistry, Dr. A.L. Mudaliar Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, India.
Subramanian
L
L
Gayathri
R
R
Angayarkanni
N
N
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002850:Chromatography, Gel; D003060:Cod Liver Oil / Q000378:metabolism; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008970:Molecular Weight; D010938:Plant Oils / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010940:Plant Proteins / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013056:Spectrophotometry, Ultraviolet; D013438:Sulfhydryl Compounds / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism / Q000494:pharmacology
D000975:Antioxidants; D010938:Plant Oils; D010940:Plant Proteins; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D003060:Cod Liver Oil; D014357:Trypsin; D000252:Calcium-Transporting ATPases; D003474:Curcumin