[Identification and immunopathogenicity of a common T cell epitope between human thyroglobulin and human thyroid peroxidase].
Human thyroglobulin (hTg) and human thyroid peroxidase (hTPO) share common B cell epitopes recognized by autoantibodies in patients with chronic autoimmune thyroiditis. Furthermore, we have demonstrated a functional common T cell epitope between hTg and hTPO in mice. Four hTg peptides in which 5 amino acid residues were identical to those of hTPO, and one hTPO peptide were prepared. Among these peptides, Tg-P4 (residues 2730-2743) and TPO-P4 (residues 118-131) shared the common T cell epitope and both peptides were highly antigenic. In addition, when the spleen cells from mice immunized with mouse Tg (mTg) were restimulated in vitro by Tg-P4 or TPO-P4, as well as by mTg, these cells transferred thyroiditis to naive recipient mice. These findings indicate that this common T cell epitope is immunogenic and related to the development of murine experimental autoimmune thyroiditis. The common T cell epitope between hTg and hTPO may play a role in the pathogenesis of human autoimmune thyroid disease.
Hoshioka
A
A
Department of Pediatrics, School of Medicine, Chiba University.
Kohno
Y
Y
Niimi
H
H
D000595:Amino Acid Sequence; D000818:Animals; D000939:Epitopes; D006801:Humans; D007453:Iodide Peroxidase / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000276:immunology*; D013954:Thyroglobulin / Q000737:chemistry / Q000276:immunology*; D013967:Thyroiditis, Autoimmune / Q000209:etiology* / Q000276:immunology
D000939:Epitopes; D013954:Thyroglobulin; D007453:Iodide Peroxidase