Purification and characterization of a specific Fasciola antigen.
Specific Fasciola antigen was prepared from homogenates of Fasciola hepatica adult worms. The homogenate was ultracentrifuged and the supernatant containing crude Fasciola antigen was then passed over a cyanogen bromide activated sepharose 4B column coupled with antiserum against Schistosoma mansoni adult worm surface antigen. The specific, Schistosoma-free Fasciola antigen was tested for its specificity by immunodiffusion. Characterization of the specific Fasciola antigen was done by gradient poly-acrylamide gel electrophoresis and immunoblotting technique. The electrophoresis migration pattern of specific Fasciola antigen, stained with Coomassie blue, showed 7 bands in the 12-54 kDa regions. Using the immunoblotting technique, a batch of positive fascioliasis sera recognized two specific bands at the 33 and 54 kDa regions.
Shaker
Z A
ZA
Department of Immunology, Theodor Bilharz Research Institute, Cairo, Egypt.
Demerdash
Z A
ZA
Mansour
W A
WA
Hassanein
H I
HI
el Mohandes
M
M
el Gindy
H I
HI
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D000947:Antigens, Helminth / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D005210:Fasciola hepatica / Q000276:immunology*; D015151:Immunoblotting; D005779:Immunodiffusion
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D000939:Epitopes