title: Murine B7-2, an alternative CTLA4 counter-receptor that costimulates T cell proliferation and interleukin 2 production.
abstract: The B7-1 molecule, expressed on antigen presenting cells (APC), provides a crucial costimulatory signal for T cell activation. Recent studies demonstrate the existence of alternative, non-B7-1 CTLA4 counter-receptors in mice and humans. Here, we describe the molecular cloning and demonstrate costimulatory function of the murine B7-2 (mB7-2) gene. Murine B7-2 cDNA encodes a member of the Ig supergene family that binds CTLA4-Ig and stains with the GL1 but not anti-mB7-1 mAb. Murine B7-2 costimulates the proliferation and interleukin 2 production of CD4+ T cells and this costimulation can be inhibited by either CTLA4-Ig or GL1 mAb. Identification of the B7-2 molecule will permit further manipulation of the B7:CD28/CTLA4 costimulatory pathway which has been shown to be involved in the prevention of tolerance, induction of tumor immunity, and most recently, in the pathogenesis of autoimmunity.
authors.affiliation: Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, Boston, MA 02115.
authors.forename: G J
authors.initials: GJ
authors.lastname: Freeman
authors.forename: F
authors.initials: F
authors.lastname: Borriello
authors.forename: R J
authors.initials: RJ
authors.lastname: Hodes
authors.forename: H
authors.initials: H
authors.lastname: Reiser
authors.forename: J G
authors.initials: JG
authors.lastname: Gribben
authors.forename: J W
authors.initials: JW
authors.lastname: Ng
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: J M
authors.initials: JM
authors.lastname: Goldberg
authors.forename: K
authors.initials: K
authors.lastname: Hathcock
authors.forename: G
authors.initials: G
authors.lastname: Laszlo
mesh_terms: D000069594:Abatacept; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000378:metabolism*; D001402:B-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D018122:B7-1 Antigen / Q000235:genetics* / Q000378:metabolism; D051940:B7-2 Antigen; D060908:CTLA-4 Antigen; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018796:Immunoconjugates; D007376:Interleukin-2 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins; D051379:Mice; D008969:Molecular Sequence Data; D011956:Receptors, Cell Surface / Q000378:metabolism*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; C497965:Cd86 protein, mouse; C556707:Ctla4 protein, mouse; D018076:DNA, Complementary; D018796:Immunoconjugates; D007376:Interleukin-2; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; D000069594:Abatacept