Cloning of a putative inhibitory amino acid receptor subunit from the parasitic nematode Haemonchus contortus.
A cDNA, HGl, encoding an inhibitory amino-acid receptor subunit has been cloned from a mixed egg population of the parasitic nematode Haemonchus contortus. The predicted amino-acid sequence of the subunit shows 24% to 32% homology with other vertebrate and invertebrate GABAA and glycine receptor subunits and has all the expected motifs for a member of the ligand-gated ion channel superfamily. When expressed in Xenopus oocytes HGl gives a small response to 1 mM glycine, but not to 1 mM GABA, glutamate, taurine or L-alanine.
Laughton
D L
DL
School of Biology and Biochemistry, University of Bath, Claverton Down, United Kingdom.
Amar
M
M
Thomas
P
P
Towner
P
P
Harris
P
P
Lunt
G G
GG
Wolstenholme
A J
AJ
D000409:Alanine / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D005260:Female; D015870:Gene Expression; D018698:Glutamic Acid / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology; D006189:Haemonchus / Q000378:metabolism*; D007448:Invertebrates; D007473:Ion Channels / Q000096:biosynthesis* / Q000502:physiology; D046911:Macromolecular Substances; D008564:Membrane Potentials / Q000187:drug effects; D008969:Molecular Sequence Data; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010063:Ovum / Q000378:metabolism; D017459:Receptors, Amino Acid / Q000096:biosynthesis* / Q000187:drug effects / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013654:Taurine / Q000494:pharmacology; D014714:Vertebrates; D014981:Xenopus; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D017931:DNA Primers; D018076:DNA, Complementary; D007473:Ion Channels; D046911:Macromolecular Substances; D017459:Receptors, Amino Acid; D013654:Taurine; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D000409:Alanine; D005998:Glycine