Comparative investigation of anticlastogenic effects in cell cultures of healthy donors and patients with nettle-rash.
In cultures of lymphocytes from 12 healthy donors and 12 patients with nettle-rash (NR) the anticlastogenic effect of the antimutagens WR-2721 (WR), bemitil (BM), tomerzol (TM) and interferon (IF) on the induction of chromosomal aberrations by photrin (PT) and dioxidine (DX) was investigated. There were no statistically significant differences between healthy donors and patients with NR in the levels of chromosomal aberrations that were spontaneous or induced by PT or DX. Statistically significant protective effects of BM, WR, TM and IF were demonstrated in cells of healthy donors after treatment with PT or DX, and after modification of the clastogenic action of PT in lymphocytes of NR patients. There was no protective effect of any of the anticlastogens after treatment of the lymphocyte cultures from NR patients with DX. That observation allows us to suggest the test of anticlastogenic action as a measure of sensitivity of the chromosomal apparatus in groups with different genetic risks.
Arutyunyan
R M
RM
Laboratory of Cytogenetics, State University, Yerevan, Armenia.
Sarkisyan
T F
TF
Oganesyan
G G
GG
Durnev
A D
AD
D004999:Amifostine / Q000494:pharmacology; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001388:Aziridines / Q000633:toxicity; D001562:Benzimidazoles / Q000494:pharmacology; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003872:Dermatitis / Q000235:genetics / Q000473:pathology*; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects*; D009153:Mutagens / Q000633:toxicity; D009154:Mutation / Q000187:drug effects*; D011810:Quinoxalines / Q000633:toxicity
D016587:Antimutagenic Agents; D001388:Aziridines; D001562:Benzimidazoles; D009153:Mutagens; D011810:Quinoxalines; C001077:dioxidine; C007779:fotrin; C049825:bemethyl; D007372:Interferons; D004999:Amifostine