title: Selective RNA editing and subunit assembly of native glutamate receptors.
abstract: RNA editing and subunit assembly of ionotropic glutamate receptors (GluRs) were examined in an oligodendrocyte progenitor cell line, CG4, which expresses GluR2-GluR4, GluR6, GluR7, KA1, and KA2. AMPA-evoked currents rapidly desensitize, whereas kainate-evoked currents contain a steady-state component with a nearly linear current-voltage relation and a fast desensitizing component that is inwardly rectifying. The Q/R site is edited > 95% to the arginine codon in GluR2(Q607) mRNA, and < 5% in GluR6(Q621) mRNA. Immunoprecipitation experiments demonstrate that GluR6 and/or GluR7 subunits assemble with KA2, but not with GluR2-GluR4. These results indicate that oligodendrocyte progenitor cells selectively edit and assemble glutamate receptors into at least two functionally and structurally distinct heteromeric channels.
authors.affiliation: Molecular Neurobiology Laboratory, Salk Institute, La Jolla, California 92037.
authors.forename: R B
authors.initials: RB
authors.lastname: Puchalski
authors.forename: J C
authors.initials: JC
authors.lastname: Louis
authors.forename: N
authors.initials: N
authors.lastname: Brose
authors.forename: S F
authors.initials: SF
authors.lastname: Traynelis
authors.forename: J
authors.initials: J
authors.lastname: Egebjerg
authors.forename: V
authors.initials: V
authors.lastname: Kukekov
authors.forename: R J
authors.initials: RJ
authors.lastname: Wenthold
authors.forename: S W
authors.initials: SW
authors.lastname: Rogers
authors.forename: F
authors.initials: F
authors.lastname: Lin
authors.forename: T
authors.initials: T
authors.lastname: Moran
mesh_terms: D000818:Animals; D001120:Arginine; D001483:Base Sequence; D002460:Cell Line; D002540:Cerebral Cortex / Q000166:cytology; D003062:Codon; D004553:Electric Conductivity; D005971:Glutamates / Q000494:pharmacology; D018698:Glutamic Acid; D006801:Humans; D007163:Immunosorbent Techniques; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology; D007608:Kainic Acid / Q000494:pharmacology; D008969:Molecular Sequence Data; D009836:Oligodendroglia / Q000187:drug effects / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000737:chemistry; D017393:RNA Editing; D012326:RNA Splicing; D051381:Rats; D017470:Receptors, Glutamate / Q000235:genetics* / Q000502:physiology; D013234:Stem Cells / Q000502:physiology; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
chemical_list: D003062:Codon; D005971:Glutamates; D007473:Ion Channels; D017470:Receptors, Glutamate; D018698:Glutamic Acid; D012313:RNA; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; D001120:Arginine; D007608:Kainic Acid