title: Identifying members of the domain Archaea with rRNA-targeted oligonucleotide probes.
abstract: Two 16S rRNA-targeted oligonucleotide probes were designed for the archaeal kingdoms Euryachaeota and Crenarchaeota. Probe specificities were evaluated by nonradioactive dot blot hybridization against selected reference organisms. The successful application of fluorescent-probe derivatives for whole-cell hybridization required organism-specific optimizations of fixation and hybridization conditions to assure probe penetration and morphological integrity of the cells. The probes allowed preliminary grouping of three new hyperthermophilic isolates. Together with other group-specific rRNA-targeted oligonucleotide probes, these probes will facilitate rapid in situ monitoring of the populations present in hydrothermal systems and support cultivation attempts.
authors.affiliation: Lehrstuhl für Mikrobiologie, Universität Regensburg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Burggraf
authors.forename: T
authors.initials: T
authors.lastname: Mayer
authors.forename: R
authors.initials: R
authors.lastname: Amann
authors.forename: S
authors.initials: S
authors.lastname: Schadhauser
authors.forename: C R
authors.initials: CR
authors.lastname: Woese
authors.forename: K O
authors.initials: KO
authors.lastname: Stetter
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D015342:DNA Probes / Q000235:genetics; D017753:Ecosystem; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D015342:DNA Probes; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S