title: Stimulatory effect of pervanadate on calcium signals and histamine secretion of RBL-2H3 cells.
abstract: We examined the effect of pervanadate on the activation of rat basophilic leukaemia (RBL-2H3) cells. The pervanadate, generated from a combination of H2O2 and vanadate (Vi), induced concomitantly protein tyrosine phosphorylation, formation of inositol 1,4,5-trisphosphate (IP3), an increase in [Ca2+]i, and histamine secretion in RBL-2H3 cells. These effects were clearly dependent on the ratio of H2O2/Vi. The secretion of histamine, IP3 formation, and sustained increase in [Ca2+]i were effectively induced by treatment of the cells with the pervanadate produced from 1 mM H2O2 and 1 mM Vi. These effects mimic the stimulatory effects of an antigen (dinitrophenylated BSA) on Ca2+ signals, histamine secretion and morphological changes. Protein tyrosine phosphorylation, formation of IP3 and transient increase in [Ca2+]i were markedly induced by the pervanadate produced from 3 mM H2O2 and 1 mM Vi. However, histamine secretion induced by the pervanadate was very low. After the pervanadate from 3 mM H2O2 and 1 mM Vi was treated with catalase, it was able to induce the [Ca2+]i increase and histamine secretion as much as the antigen did. This indicates that pervanadate from a lower H2O2 concentration (1 mM H2O2/1 mM Vi) and catalase-treated pervanadate from a higher H2O2 concentration (3 mM H2O2/1 mM Vi) are able to mimic the activity that was caused by cross-linking of IgE receptors with antigen. The present results also demonstrate that protein tyrosine phosphorylation seems to have a crucial role in Ca2+ entry from the external medium, and that a sustained [Ca2+]i increase is an important step for histamine secretion in RBL-2H3 cells.
authors.affiliation: National Institute of Health Sciences, Tokyo, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Teshima
authors.forename: H
authors.initials: H
authors.lastname: Ikebuchi
authors.forename: M
authors.initials: M
authors.lastname: Nakanishi
authors.forename: J
authors.initials: J
authors.lastname: Sawada
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D015471:Leukemia, Basophilic, Acute / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation / Q000187:drug effects; D019000:Phosphotyrosine; D017027:Protein Tyrosine Phosphatases / Q000037:antagonists & inhibitors*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism; D014638:Vanadates / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C076715:pervanadate; D019000:Phosphotyrosine; D014638:Vanadates; D014443:Tyrosine; D015544:Inositol 1,4,5-Trisphosphate; D006861:Hydrogen Peroxide; D002374:Catalase; D017027:Protein Tyrosine Phosphatases; D002118:Calcium