Safety and immunogenicity of a polyvalent Escherichia coli vaccine in human volunteers.
Since a limited number of O serogroups account for nearly 70% of bacteremic and meningitic Escherichia coli isolates, a polyvalent vaccine was made by conjugating a Pseudomonas aeruginosa exotoxin A carrier protein to the O polysaccharide of 12 serogroups of E. coli (O1, O2, O4, O6-O8, O12, O15, O16, O18, O25, O75). No serious reactions occurred in 88 vaccinees. Four-fold or greater increases in ELISA antibody levels over baseline were greatest (> 60% of vaccinees) for O1, O2, O6-O8 and O15; intermediate (approximately 50%) for O18 and O75, and poorest (> or = 45%) for O4, O12, O16, and O25. Responses with functionally active opsonophagocytic antibody generally paralleled ELISA antibody responses. With the availability of a safe, immunogenic E. coli vaccine, active and passive immunization strategies merit further development as adjunctive treatment for E. coli bacteremia and neonatal meningitis.
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Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, DC 20307-5100.
Artenstein
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Que
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Fredeking
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Furer
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Sadoff
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JC
Cryz
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D036002:ADP Ribose Transferases; D000907:Antibodies, Bacterial / Q000097:blood; D000917:Antibody Formation; D001427:Bacterial Toxins; D001428:Bacterial Vaccines / Q000276:immunology* / Q000633:toxicity*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000276:immunology* / Q000517:prevention & control; D005098:Exotoxins; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology*; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000633:toxicity; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D005098:Exotoxins; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D011993:Recombinant Fusion Proteins; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A