Structure of the murine VCAM1 gene.
The architecture of the murine VCAM1 gene, encoding vascular cell adhesion molecule-1, was determined. its 10 exons span approximately 20 kb. Exon 1 encodes the 5' untranslated region and the signal peptide; exons 2-4 and 6-9, the C2 or H-type immunoglobulin domains; and exon 10, the transmembrane and cytoplasmic domains followed by the entire 3' untranslated region. All immunoglobulin-like domains are encoded by separate exons, and exon splice junctions occur after the first nucleotide of amino acid codons (type 1). Exon 5 encodes a novel domain unique to murine VCAM-1 and inclusion of this exon by alternative splicing results in a truncated three-immunoglobulin-like domain form, which is bound to the cell membrane by a phosphatidylinositol linkage at its carboxy terminus (encoded by exon 5). The murine VCAM1 core promoter contains a high degree of homology to the human, including conserved consensus binding sites for NF-kappa B, the Ets class, and the GATA family of transcription factors, suggesting that the murine and human VCAM1 genes may be under similar transcriptional control.
Cybulsky
M I
MI
Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Allan-Motamed
M
M
Collins
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics*; D004247:DNA; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D019010:Vascular Cell Adhesion Molecule-1
D015815:Cell Adhesion Molecules; D019010:Vascular Cell Adhesion Molecule-1; D004247:DNA