title: Myelin basic protein purification in non denaturing conditions.
abstract: The utilization of denaturing methods for protein purification causes the irreversible loss of quaternary and tertiary structure together with consistent changes in the secondary structure. These modifications reflect on protein antigenicity. MBP is a myelin protein which is bound to membrane-phospholipids. Its tertiary structure is specific for this kind of interaction which determines its native conformation. MBP was obtained in two forms: denatured and non denatured. The latter has been purified using the non-ionic detergent beta-octil-D-glucopyranoside which is able to preserve protein tertiary structure separating it from the bilayer phospholipids. Non denaturated MBP could be useful in antibody and/or lymphocyte activity detection studies in various human pathological processes.
authors.affiliation: Neurological Institute, IRCCS Foundation C. Mondino.
authors.forename: G
authors.initials: G
authors.lastname: Giardini
authors.forename: L
authors.initials: L
authors.lastname: Lorusso
authors.forename: L
authors.initials: L
authors.lastname: Barletta
authors.forename: A
authors.initials: A
authors.lastname: Malaspina
authors.forename: W
authors.initials: W
authors.lastname: Bolzani
authors.forename: F
authors.initials: F
authors.lastname: Savoldi
authors.forename: M
authors.initials: M
authors.lastname: Ceroni
mesh_terms: D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation; D002853:Chromatography, Liquid; D003902:Detergents; D017886:Durapatite; D004591:Electrophoresis, Polyacrylamide Gel; D005960:Glucosides; D004676:Myelin Basic Protein / Q000737:chemistry / Q000302:isolation & purification*; D016548:Prosencephalon / Q000737:chemistry; D011489:Protein Denaturation; D017434:Protein Structure, Tertiary
chemical_list: D003902:Detergents; D005960:Glucosides; D004676:Myelin Basic Protein; C018619:octyl-beta-D-glucoside; D017886:Durapatite