Common distribution of antigenic determinants and complementation activity on matrix proteins of two vesicular stomatitis virus serotypes.
To compare the antigenic and functional domains of the matrix (M) proteins of vesicular stomatitis virus (VSV) serotypes Indiana (VSV-Ind) and New Jersey (VSV-NJ), deletion mutants and chimeras were cloned in pBSM13 and expressed as in-frame lacZ fusion proteins in Escherichia coli. Non-cross-reactive monoclonal antibodies directed to the two antigenically distinct M proteins were tested by Western blot analysis to map three epitopes of VSV-Ind M protein and four epitopes of VSV-NJ M protein. Epitope 1 of the VSV-Ind M protein and epitope II of the VSV-NJ M protein both mapped to the highly basic N-terminal 34 amino acids of each homotypic M protein. Epitopes 2 and 3 of the VSV-Ind M protein and epitopes III and IV of the VSV-NJ M protein mapped to a region spanning amino acids 35 to 74. Epitope I of the VSV-NJ M protein mapped to a region between amino acid 75 and the C terminus. The similarity in location of the serotypically unique antigenic determinants of the two M proteins suggested that they may have a common functional domain. This hypothesis was substantiated by the finding that the two M proteins and various chimeras expressed in CV-1 cells by a recombinant vaccinia virus system were able to rescue M gene temperature-sensitive mutants of both VSV serotypes.
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Department of Microbiology, University of Virginia School of Medicine, Charlottesville 22908.
Huang
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Wagner
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D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D003001:Cloning, Molecular; D000939:Epitopes / Q000276:immunology*; D005814:Genes, Viral / Q000235:genetics; D005816:Genetic Complementation Test; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D017384:Sequence Deletion / Q000235:genetics; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics / Q000276:immunology*; D018116:Vesiculovirus; D014763:Viral Matrix Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D011993:Recombinant Fusion Proteins; D014763:Viral Matrix Proteins