Comparison of protein synthesis profiles in chronic lymphocytic leukaemia cells and B-lymphocytes from peripheral blood, cord blood and tonsil.
2D-gel electrophoresis was used to investigate protein synthesis in leukaemic cells from a series of 15 chronic lymphocytic leukaemia (CLL) patients, and in non-malignant B-cell populations from different sources. The protein synthesis profiles of CD5+ B-cells from umbilical cord blood and from tonsil were determined, and the levels of expression of their proteins were observed to be similar to the CLL cells. The CD5- cells from cord blood resembled peripheral blood B-lymphocytes, and the protein synthesis profile of CD5- cells from tonsils was very complex. One protein was also identified which consistently appeared to be synthesised at a low level in CD5+ B-cells from tonsil but which was always more prominent in CLL cells and other non-malignant B-lymphocytes. On the basis of these data it is possible that the closest non-malignant counterpart to CLL is the CD5+ B-lymphocyte from cord blood.
Saunders
F K
FK
Institute for Cancer Studies, University Medical School, Sheffield, U.K.
Lawry
J
J
Winfield
D A
DA
Goepel
J R
JR
Hancock
B W
BW
Sharrard
R M
RM
Goyns
M H
MH
D015703:Antigens, CD / Q000032:analysis / Q000276:immunology; D001345:Autoradiography; D016175:B-Lymphocyte Subsets / Q000378:metabolism; D001402:B-Lymphocytes / Q000378:metabolism*; D018956:CD5 Antigens; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005312:Fetal Blood / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D014066:Palatine Tonsil / Q000276:immunology / Q000378:metabolism; D011247:Pregnancy; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D013462:Sulfur Radioisotopes
D015703:Antigens, CD; D018956:CD5 Antigens; D009363:Neoplasm Proteins; D011506:Proteins; D013462:Sulfur Radioisotopes; D008715:Methionine