Effects of calcium-binding proteins on histamine release from permeabilized rat peritoneal mast cells.
Among the calcium-binding proteins from chicken gizzard smooth muscle, smooth muscle calcium-binding protein 11 (SMCaBP 11), which is an EF-hand type calcium-binding protein, can prevent Ca2+ dependent histamine release from permeabilized rat peritoneal mast cells. This activity appears to be specific since other calcium-binding proteins (CaBPs), including h-calcimedin, l-calcimedin, calmodulin and S100, had no inhibitory action. Approximately 40% of the histamine release was inhibited by SMCaBP 11 at 48 nM and the half-maximal inhibition occurred at 20 nM. Immunological techniques revealed that permeabilized mast cells elicited by Ca2+ also released actin into the medium, but not tubulin. The addition of SMCaBP 11 prevented the secretion of actin from the mast cells, though the distribution of F-actin was only slightly affected. These findings suggest that SMCaBP 11 may modulate Ca2+ and actin-mediated exocytosis from permeabilized mast cells.
Fujii
T
T
Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Kuzumaki
N
N
Ogoma
Y
Y
Kondo
Y
Y
D000199:Actins / Q000378:metabolism; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D018797:Cell Cycle Proteins; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel; D004815:Epidermal Growth Factor / Q000378:metabolism*; D006636:Histamine Release / Q000187:drug effects*; D015151:Immunoblotting; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D000075383:S100 Calcium Binding Protein A6; D009418:S100 Proteins; C489133:S100A6 protein, Gallus gallus; C489132:S100a6 protein, rat; D014404:Tubulin; D000001:Calcimycin; D004815:Epidermal Growth Factor; D002118:Calcium