Influence of lindane and paraquat on oxidative stress-related parameters of erythrocytes in vitro.
1. The influence of lindane and paraquat on oxidative stress-related parameters of the red blood cell was studied in vitro. 2. Lindane addition did not modify either the t-butyl hydroperoxide-induced oxygen uptake of the erythrocytes and the induction time preceding it, or the activity of catalase, superoxide dismutase, glutathione peroxidase and glucose 6-phosphate dehydrogenase, in conditions of comparable levels of haemoglobin and methaemoglobin. 3. Red blood cells exposed to paraquat exhibited a concentration-dependent decrease in the t-butyl hydroperoxide-induced oxygen consumption and increments in either the induction period or in the activity of catalase and glucose 6-phosphate dehydrogenase, with no changes in superoxide dismutase activity and a small decrement in that of glutathione peroxidase. 4. These data indicate that lindane does not interfere with the oxidant status of the erythrocyte, while paraquat addition leads to an increment in the anti-oxidant capacity of the red blood cell.
Bainy
A C
AC
Instituto de Quimica, Universidade de São Paulo, Brasil.
Silva
M A
MA
Kogake
M
M
Videla
L A
LA
Junqueira
V B
VB
D000818:Animals; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D006454:Hemoglobins / Q000187:drug effects; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D066298:In Vitro Techniques; D008297:Male; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D010269:Paraquat / Q000633:toxicity*; D010545:Peroxides / Q000494:pharmacology; D051381:Rats; D020122:tert-Butylhydroperoxide
D006454:Hemoglobins; D016877:Oxidants; D010545:Peroxides; D001556:Hexachlorocyclohexane; D020122:tert-Butylhydroperoxide; D010269:Paraquat