title: Expression of alpha 4 integrin mRNA and protein and fibronectin in the early chicken embryo.
abstract: alpha 4 integrins (alpha 4 beta 1 and alpha 4 beta 7) have been shown to mediate both cell-matrix adhesion to fibronectin and cell-cell adhesion to VCAM-1. These interactions have been suggested to contribute to hematopoiesis, lymphocyte homing, recruitment of inflammatory cells, neural crest cell migration and myogenesis. We report here the cloning of chicken alpha 4 cDNA and its use to define the patterns of expression of alpha 4 mRNA and protein in early chicken embryos (19-22 somite pairs), a stage at which neural crest cells can be examined at various points in their migration and somitic development and differentiation can also be observed at various stages. We observe widespread expression of both alpha 4 mRNA and protein, although the patterns of steady state expression do not conform precisely. Many neural crest cells contain significant levels of alpha 4 mRNA. Some neural crest cells express alpha 4 protein but its expression is transient and/or limited to a subset of these cells. alpha 4 is strongly expressed at both mRNA and protein levels by somitic cells and their derivatives in the sclerotome, dermatome and myotome and is also expressed in neural tube, otic placode, heart, gut endoderm and some other tissues. Comparison with the distributions of fibronectin shows that, although some alpha 4 expression occurs in locations consistent with a role in cell-matrix adhesion to fibronectin, alpha 4 is also expressed in other places where fibronectin is low or absent and a role for alpha 4 in cell-cell interactions appears more likely.
authors.affiliation: Center for Cancer Research, Massachusetts Institute of Technology, Cambridge.
authors.forename: M A
authors.initials: MA
authors.lastname: Stepp
authors.forename: L A
authors.initials: LA
authors.lastname: Urry
authors.forename: R O
authors.initials: RO
authors.lastname: Hynes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D018959:CD57 Antigens; D002642:Chick Embryo; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005353:Fibronectins / Q000096:biosynthesis*; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology; D006801:Humans; D017403:In Situ Hybridization; D039441:Integrin alpha4; D016023:Integrins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009432:Neural Crest / Q000378:metabolism; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018959:CD57 Antigens; D018076:DNA, Complementary; D005353:Fibronectins; D016023:Integrins; D009842:Oligopeptides; D012333:RNA, Messenger; D039441:Integrin alpha4