title: Expression and analysis of the NS2 protein of influenza A virus.
abstract: Influenza NS2 protein was expressed in Saccharomyces cerevisiae using a copper-inducible promoter. The protein produced had a molecular weight of 13 kDa, was reactive with anti-NS2 antiserum and was localised to the yeast cell nucleus. Two-hybrid analysis identified a direct protein-protein interaction between NS2 and the M2 protein of the virus, involving the C-terminal 163 residues of M1. A filter-binding assay localised the M1 binding region to the C-terminal 70 amino acids of NS2.
authors.affiliation: Biomolecular Research Institute, Parkville, Victoria, Australia.
authors.forename: A C
authors.initials: AC
authors.lastname: Ward
authors.forename: L A
authors.initials: LA
authors.lastname: Castelli
authors.forename: A C
authors.initials: AC
authors.lastname: Lucantoni
authors.forename: J F
authors.initials: JF
authors.lastname: White
authors.forename: A A
authors.initials: AA
authors.lastname: Azad
authors.forename: I G
authors.initials: IG
authors.lastname: Macreadie
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D009980:Influenza A virus / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011485:Protein Binding; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae; D014763:Viral Matrix Proteins / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D000086082:Viroporin Proteins
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins; D017361:Viral Nonstructural Proteins; C031540:M-protein, influenza virus; C488936:M1 protein, Influenza A virus; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins