title: Functional complementation of the radiation-sensitive mutant M10 cell line by human chromosome 5.
abstract: The mouse lymphoma (L5178Y) cell mutant M10 is defective in rejoining DNA double-strand breaks and is hypersensitive to ionizing radiation. The introduction of human chromosome 5 into M10 cells by microcell mediated chromosome transfer complemented the ionizing-radiation hypersensitivity defect of this cell line. The presence of chromosome 5 in the microcell hybrids was shown using PCR with chromosome-specific primers and fluorescence in situ hybridization. From this data we conclude that the gene that corrects the radiation hypersensitivity of M10 cells is located on chromosome 5 and tentatively assigned to the 5q14 to 5pter region. We designate this gene XRCC4L.
authors.affiliation: Life Sciences Division, MS-M888, Los Alamos National Laboratory, NM 87545.
authors.forename: M A
authors.initials: MA
authors.lastname: Stackhouse
authors.forename: J B
authors.initials: JB
authors.lastname: Ortiz
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: D J
authors.initials: DJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D002895:Chromosomes, Human, Pair 5; D004260:DNA Repair / Q000235:genetics; D005816:Genetic Complementation Test; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D009154:Mutation; D016133:Polymerase Chain Reaction; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured