Isolation of a monoclonal antibody which blocks vaccinia virus infection.
We have isolated a monoclonal antibody, B2, that neutralizes vaccinia virus infection. B2 reacts with a trypsin-sensitive cell surface epitope. B2 does not neutralize infection of herpes simplex virus, suggesting that the B2-reactive epitope is specifically involved in vaccinia virus entry. A survey of 12 different cell lines reveals a correlation between B2 reactivity and susceptibility to vaccinia virus infection. In addition, B2 interferes with vaccinia virus adsorption to target cells. Taken together, the B2-reactive epitope is part of a receptor that appears important for vaccinia virus entry.
Chang
W
W
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
Hsiao
J C
JC
Chung
C S
CS
Bair
C H
CH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000939:Epitopes / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009500:Neutralization Tests; D014615:Vaccinia / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes