title: Dissociation of immune determinants of outer membrane proteins of Chlamydia psittaci strain guinea pig inclusion conjunctivitis.
abstract: Chlamydia trachomatis is an important human pathogen. Research to develop a Chlamydia vaccine has focused on the major outer membrane protein (MOMP). Determinants of this protein elicit serovar-specific neutralizing antibodies which are thought to play a critical role in protective immunity. MOMP-specific antibody responses are highly variable in the polymorphic population. Genetic factors which might influence the MOMP-specific immune response are consequently of particular interest. The C. psittaci strain guinea pig inclusion conjunctivitis (GPIC) is a natural pathogen of the guinea pig that causes both ocular and genital tract infections that closely resemble those caused by C. trachomatis in humans. As such, it provides an excellent model for disease. In this report, we explore the influence of major histocompatibility complex-linked genes on the MOMP-specific antibody response in mice immunized with either whole GPIC elementary bodies or recombinant GPIC MOMP. Our results indicate that the MOMP-specific antibody response is major histocompatibility complex linked such that mice of the H-2d haplotype are high responders while mice of the H-2k haplotype are low responders. We demonstrate that MOMP-specific B cells are present in H-2k strains which are, however, deficient in MOMP-specific helper T cells. Although immunization of low-MOMP-responder strains with whole chlamydial elementary bodies induces high levels of immunoglobulin G antibody specific for Omp2, the cysteine-rich outer membrane protein, MOMP-specific B cells are unable to receive help from Omp2-specific T cells. The failure of intermolecular help from Omp2-specific T cells and related observations raise important issues regarding the processing and presentation of chlamydial antigens and the design of optimal subunit vaccines.
authors.affiliation: Department of Microbiology, University of Rochester Medical Center, New York 14642.
authors.forename: T D
authors.initials: TD
authors.lastname: Westbay
authors.forename: C C
authors.initials: CC
authors.lastname: Dascher
authors.forename: R C
authors.initials: RC
authors.lastname: Hsia
authors.forename: P M
authors.initials: PM
authors.lastname: Bavoil
authors.forename: M
authors.initials: M
authors.lastname: Zauderer
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D002691:Chlamydophila psittaci / Q000145:classification / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006168:Guinea Pigs; D006183:H-2 Antigens / Q000235:genetics*; D006239:Haplotypes; D016056:Immunodominant Epitopes / Q000276:immunology; D008285:Major Histocompatibility Complex / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D018272:Porins; D011994:Recombinant Proteins / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D014611:Vaccination
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D006183:H-2 Antigens; D016056:Immunodominant Epitopes; C063931:Omp2 protein, bacteria; D010446:Peptide Fragments; D018272:Porins; D011994:Recombinant Proteins; C071677:omp1 protein, Chlamydia trachomatis