Cell cycle dynamics of microcarrier cultures.
In anchorage-dependent cell microcarrier cultures knowledge of the cell's growth kinetics is necessary in order to design and successfully operate bioreactors, particularly on a large scale. However, in addition to growth kinetics, an understanding of the physiological state of the culture is also important. In this paper the cell cycle progression of Vero and MRC-5 microcarrier cultures have been observed utilizing a flow cytometer. Flow cytometry analysis enabled the differentiation of the various phases of the cell cycle as the culture moved from initial inoculation to the stationary, or confluent stage. Not only was the flow cytometer able to distinguish contact inhibited cells from noncontact inhibited cells, but the measured fraction of contact inhibition cells were found to be in agreement with fractions predicted from a previously developed cellular automation model for microcarrier cultures. Further, the data from the stationary phase was used to quantify the death rate in microcarrier cultures.
Hawboldt
K A
KA
Pharmaceutical Production Research Facility (PPRF), Faculty of Engineering, University of Calgary, Alberta, Canada.
Linardos
T I
TI
Kalogerakis
N
N
Behie
L A
LA
D000818:Animals; D001331:Automation; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis; D005333:Fetus; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007700:Kinetics; D008168:Lung; D012313:RNA / Q000032:analysis; D013997:Time Factors; D014709:Vero Cells
D012313:RNA; D004247:DNA