title: Antigenic mimicry of Clostridium chauvoei flagella by polyclonal anti-idiotypic antibodies.
abstract: Polyclonal rabbit anti-idiotypic (anti-Id) antibodies against two monoclonal antibodies (MAbs) specific for the flagella of Clostridium chauvoei were produced, purified and characterised. Lack of cross-reactivity with heterologous MAbs indicated that the anti-Id antibodies were highly specific. The surface-exposed epitopes of the flagellar filament recognised with protective MAb were further distinguished by the anti-Id antibodies. Moreover, each anti-Id antibody inhibited the binding of its related MAb to flagellar antigens in a competitive enzyme-linked immunosorbent assay, suggesting that the anti-Id antibodies bore an internal image of the flagellar antigens. The survival rate of mice was increased to nearly twice that of controls by immunisation with anti-Id 41, which had been produced with a protective MAb; in contrast, anti-Id 114, produced with a non-protective MAb, failed to immunise. The results suggest that an anti-Id antibody containing an internal image of C. chauvoei flagella might be used as a vaccine.
authors.affiliation: National Veterinary Assay Laboratory, Tokyo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kijima-Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Tamura
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: N
authors.initials: N
authors.lastname: Nagamine
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis / Q000276:immunology* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D003013:Clostridium / Q000276:immunology* / Q000648:ultrastructure; D003015:Clostridium Infections / Q000401:mortality / Q000517:prevention & control; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D005260:Female; D005407:Flagella / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D015996:Survival Rate
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes