Characterization of the Spodoptera frugiperda TATA-binding protein: nucleotide sequence and response to baculovirus infection.
A cDNA clone containing a 921 bp open-reading frame (307 amino acids; 34 kDa) homologous to the TATA-binding protein (TBP) was isolated and sequenced from a Spodoptera frugiperda cell line that is commonly used in the baculovirus expression system. Analysis of the S. frugiperda TBP (SfTBP) sequence showed that the amino-terminal portion of SfTBP diverged significantly from that of other TBP sequences including Drosophila melanogaster whereas the carboxy-terminal sequence was highly conserved. Southern blot analysis indicated that SfTBP was encoded by a single gene in the S. frugiperda genome. Northern blot analysis indicated that steady-state levels of the 1.3 kb SfTBP transcript declined by 24 h post-infection corresponding to the time of virus-induced inhibition of host-cell transcription. Corresponding western blot analysis showed that TBP protein levels remain constant up to 72 h post-infection.
Rasmussen
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Department of Agricultural Chemistry, Oregon State University, Corvallis 97331-7301.
Rohrmann
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GF
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000502:physiology*; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics / Q000378:metabolism; D014644:Genetic Variation; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D016366:Open Reading Frames; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D016385:TATA Box; D035181:TATA-Box Binding Protein; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
D017931:DNA Primers; D004268:DNA-Binding Proteins; D035181:TATA-Box Binding Protein; D014157:Transcription Factors; D012313:RNA