Determination of the synthesis and uptake of alpha 2-macroglobulin by cultured human glioma cells.
Using immunological techniques, the synthesis of alpha 2-macroglobulin was studied in established cell lines derived from human glioblastomas multiforme. alpha 2-Macroglobulin was detected in cytoplasm and in the culture medium of the analyzed cell lines. Radioimmunoprecipitation revealed a protein with M(r) corresponding to alpha 2-macroglobulin in the medium conditioned by U-118MG and U-343MG cells. On the other hand, using immunoblot analysis, alpha 2-macroglobulin was detected in all of the analyzed lines. In immunofluorescence test, alpha 2-macroglobulin was determined also in all four cell lines, but with different staining pattern. Conditioned culture medium (CCM) of U-536MG cells with the lowest level of alpha 2-macroglobulin exerted the lowest mitogenic activity for human fibroblasts.
Drusková
E
E
Department of Physiology, School of Medicine, Comenius University, Bratislava, Slovakia.
Bízik
J
J
Grófová
M
M
D015153:Blotting, Western; D017077:Culture Media, Conditioned; D003593:Cytoplasm / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005909:Glioblastoma / Q000378:metabolism*; D006801:Humans; D008938:Mitosis; D011233:Precipitin Tests; D014407:Tumor Cells, Cultured; D000511:alpha-Macroglobulins / Q000096:biosynthesis*
D017077:Culture Media, Conditioned; D000511:alpha-Macroglobulins