title: Lipopolysaccharide O-antigen biosynthesis in Shigella dysenteriae serotype 1: analysis of the plasmid-carried rfp determinant.
abstract: The O-antigen polysaccharide of the lipopolysaccharide of Shigella dysenteriae serotype 1 is encoded by determinants located on a 9 kb plasmid (rfp) and on the chromosome near the his locus (rfb). Molecular genetic and biochemical studies of the rfp determinant reported here show that the rfp region contains two genes, rfpA and rfpB, lying in an operon. rfpB was demonstrated to encode a membrane-bound galactosyl-transferase. The low G+C content of rfp DNA suggests that it did not originate in Shigella.
authors.affiliation: Department of Microbiology, GBF-National Research Centre for Biotechnology, Braunschweig, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Göhmann
authors.forename: P A
authors.initials: PA
authors.lastname: Manning
authors.forename: C A
authors.initials: CA
authors.lastname: Alpert
authors.forename: M J
authors.initials: MJ
authors.lastname: Walker
authors.forename: K N
authors.initials: KN
authors.lastname: Timmis
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002240:Carbohydrate Sequence; D005700:Galactosyltransferases / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D019081:O Antigens; D010957:Plasmids / Q000235:genetics; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012703:Serotyping; D012762:Shigella dysenteriae / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic
chemical_list: D019081:O Antigens; D011135:Polysaccharides, Bacterial; D011994:Recombinant Proteins; D005700:Galactosyltransferases