title: Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa.
abstract: Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.
authors.affiliation: Department of Molecular Biology, Odense University, Denmark.
authors.forename: F
authors.initials: F
authors.lastname: Kirpekar
authors.forename: E
authors.initials: E
authors.lastname: Nordhoff
authors.forename: K
authors.initials: K
authors.lastname: Kristiansen
authors.forename: P
authors.initials: P
authors.lastname: Roepstorff
authors.forename: A
authors.initials: A
authors.lastname: Lezius
authors.forename: S
authors.initials: S
authors.lastname: Hahner
authors.forename: M
authors.initials: M
authors.lastname: Karas
authors.forename: F
authors.initials: F
authors.lastname: Hillenkamp
mesh_terms: D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D012313:RNA / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry; D017423:Sequence Analysis, RNA
chemical_list: D012313:RNA; D012321:DNA-Directed RNA Polymerases; D010727:Phosphoric Diester Hydrolases