title: Migration of Thy-1+ dendritic epidermal cells (Thy-1+DEC): Ly48 and TNF-alpha are responsible for the migration of Thy-1+DEC to the epidermis.
abstract: Thy-1+ dendritic epidermal cells (Thy-1+DEC) are mainly T cells that express T-cell receptor gamma and delta chains with limited diversity of gamma delta, mainly gamma 3 delta 1; such gamma 3 delta 1 TCR-bearing Thy-1+DEC originate from day 16 fetal thymic cells. To understand the migratory capability of Thy-1+DEC, we developed an in vitro model, using skin organ culture. First, emigration of Thy-1+DEC from the epidermis was examined. Ear skin from C3H/He mice was separated into two parts and incubated for 3 d with dermal side down. Thy-1+DEC emigrated from the epidermis into the dermis and then migrated out of the skin into the culture medium. Next, immigration of Thy-1+DEC into the epidermis was examined. Thy-1+DEC were depleted in vivo by daily application of clobetazole propionate solution topically onto the ears of C3H/He mice. Seven days later, ear skin was harvested, separated, and cultured with the dermal side up with syngeneic epidermal cell suspensions with a migration chamber for 3 d. It was found that 1) Thy-1+DEC immigrated into the Thy-1+DEC depleted epidermis as well as into untreated epidermis, and 2) the migratory capability of Thy-1+DEC was directly proved by a biolabeling technique with PKH-26. Blocking studies with various antibodies revealed that leukosialin (S11 monoclonal antibodies) and TNF alpha were important for Thy-1+DEC migration. Thus, Thy-1+DEC retain the potential for migration in vitro, and leukosialin and TNF alpha are partially responsible for the migration of Thy-1+DEC into the epidermis.
authors.affiliation: Department of Dermatology, Yamanashi Medical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Tamaki
authors.forename: A
authors.initials: A
authors.lastname: Saitoh
authors.forename: A A
authors.initials: AA
authors.lastname: Gaspari
authors.forename: N
authors.initials: N
authors.lastname: Yasaka
authors.forename: M
authors.initials: M
authors.lastname: Furue
mesh_terms: D000818:Animals; D015703:Antigens, CD; D000954:Antigens, Surface / Q000032:analysis*; D002465:Cell Movement; D002990:Clobetasol / Q000031:analogs & derivatives / Q000494:pharmacology; D003713:Dendritic Cells / Q000276:immunology* / Q000502:physiology*; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects; D000939:Epitopes; D005456:Fluorescent Dyes; D051917:Leukosialin; D008562:Membrane Glycoproteins / Q000032:analysis*; D051379:Mice; D008809:Mice, Inbred C3H; D009924:Organ Culture Techniques; D009930:Organic Chemicals; D012016:Reference Values; D012795:Sialoglycoproteins / Q000502:physiology*; D012867:Skin / Q000166:cytology; D018800:Thy-1 Antigens; D014409:Tumor Necrosis Factor-alpha / Q000502:physiology*
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D000939:Epitopes; D005456:Fluorescent Dyes; D051917:Leukosialin; D008562:Membrane Glycoproteins; D009930:Organic Chemicals; C070080:PKH 26; D012795:Sialoglycoproteins; C497863:Spn protein, mouse; D018800:Thy-1 Antigens; D014409:Tumor Necrosis Factor-alpha; D002990:Clobetasol