The superiority of UW solution for maintaining ATP concentrations during pulmonary preservation.
We evaluated three solutions used for preserving lungs, namely, University of Wisconsin (UW), Euro-Collins (E-C), and low potassium dextran (LPD), by measuring the high energy phosphates in the preserved lung tissue. The left lungs of Sprague-Dawley rats were excised and flushed with 5 ml of one of the solutions at 10 degrees C through the pulmonary artery, after which they were deflated and immersed in the solution at 10 degrees C for 24 h. The tissue adenosine triphosphate (ATP) concentration in mumol/g tissue wet weight after 24 h of storage was 2.55 +/- 0.48 (n = 7) in the UW lungs, 1.98 +/- 0.25 (n = 6) in the E-C lungs, and 1.53 +/- 0.32 (n = 4) in the LPD lungs, being significantly higher in the UW lungs than in either the E-C or LPD lungs (P < 0.05). The histopathological findings of the E-C lungs were more deteriorated, with marked interstitial edema, septal hypertrophy, and perivascular hyaline degeneration, than either the UW or LPD lungs. Thus, the findings of this study indicate the superiority of UW solution for lung preservation.
Omote
Y
Y
First Department of Surgery, Asahikawa Medical College, Japan.
Ikeda
K
K
Yamazaki
K
K
Kubo
Y
Y
D000227:Adenine Nucleotides / Q000032:analysis; D000241:Adenosine; D000255:Adenosine Triphosphate / Q000032:analysis*; D000493:Allopurinol; D000818:Animals; D003911:Dextrans; D005978:Glutathione; D006982:Hypertonic Solutions; D007328:Insulin; D008168:Lung / Q000737:chemistry* / Q000473:pathology; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D011887:Raffinose; D051381:Rats; D017207:Rats, Sprague-Dawley
D000227:Adenine Nucleotides; D003911:Dextrans; C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D000255:Adenosine Triphosphate; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose