Detection and characterization of antibodies to bacterial heat-shock protein 60 in sera of patients with primary biliary cirrhosis.
The enzyme-linked immunosorbent assay (ELISA) with bacterial heat-shock protein 60 (HSP60) purified from Yersinia enterocolitica (Ye) revealed that the antibodies directed against YeHSP60 existed in sera of patients with primary biliary cirrhosis (PBC). To characterize the epitope specificity of the antibodies in patients, the epitope mapping of HSP60 by means of the antibodies was performed. The results have suggested that the epitope recognized with anti-HSP60 antibodies in PBC relates to the amino acid sequence of YeHSP60 molecule as follows: DLGQAKRVVINKDTTIIIDGVGDEAAIQGRLAQIRQQIEEATSDYDKEK.
Yamaguchi
H
H
Department of Microbiology, Kyorin University School of Medicine, Tokyo, Japan.
Miura
H
H
Ohsumi
K
K
Ishimi
N
N
Taguchi
H
H
Ishiyama
N
N
Shiraishi
Y
Y
Yamamoto
T
T
Ogata
S
S
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D018834:Chaperonin 60 / Q000235:genetics / Q000276:immunology*; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008105:Liver Cirrhosis, Biliary / Q000097:blood* / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D000911:Antibodies, Monoclonal; D018834:Chaperonin 60; D017931:DNA Primers; D000939:Epitopes; D007074:Immunoglobulin G