title: Adhesion and penetration properties of human lymphocytes acting on allogeneic vascular endothelial cells.
abstract: Lymphocyte infiltration through vascular endothelium is one important step in the course of graft rejection. To investigate this process more exactly we established a monolayer invasion assay which enabled us to discriminate between adherent and penetrated cells. Detailed studies of adhesion and penetration kinetics of peripheral blood lymphocytes (PBL) acting on allogeneic human umbilical vein endothelial cells (HUVEC) were carried out by combined phase contrast and reflection interference contrast microscopy. Between 30 and 35% of all PBL attached to HUVEC after 4 hr. Out of these less than 10% penetrated. When HUVEC were prestimulated for 2 hr by interferon (IFN)-alpha,-beta,-gamma or interleukin (IL)-1, PBL adhesion in the early phase of cellular attachment to endothelial cells was accelerated. Overall adhesion however did not increase. Long-term pretreatment of HUVEC for 72 hr with IFN-gamma or IL-1 also modified PBL-HUVEC interactions. However, a 72-hr pretreatment with IFN-alpha or -beta did not influence lymphocyte binding behaviour. PBL penetration was not only accelerated but also enhanced by IFN-alpha,-beta,-gamma, irrespective of whether HUVEC were prestimulated for 2 hr or PBL and cytokines were added simultaneously to HUVEC. On the other hand IL-1 was not able to enhance the amount of penetrated cells but only accelerated the infiltration process. Up-regulation or de novo expression of the adhesion molecules ICAM-1 (intercellular adhesion molecule), ELAM-1 (endothelial leucocyte adhesion molecule) and VCAM-1 (vascular cell adhesion molecule) did not parallel PBL binding kinetics. Therefore an ICAM-, ELAM- and VCAM-independent modulation in the early phase of lymphocyte attachment to endothelium seems likely. The lymphocyte cytoskeleton may have a role in this process.
authors.affiliation: Department of General Surgery, Hospital of J. W. Goethe University, Frankfurt/Main, Germany.
authors.forename: R A
authors.initials: RA
authors.lastname: Blaheta
authors.forename: M
authors.initials: M
authors.lastname: Scholz
authors.forename: N P
authors.initials: NP
authors.lastname: Hailer
authors.forename: J
authors.initials: J
authors.lastname: Bereiter-Hahn
authors.forename: A
authors.initials: A
authors.lastname: Encke
authors.forename: B H
authors.initials: BH
authors.lastname: Markus
mesh_terms: D002448:Cell Adhesion / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000276:immunology*; D006084:Graft Rejection / Q000276:immunology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D007372:Interferons / Q000276:immunology; D007375:Interleukin-1 / Q000276:immunology; D007700:Kinetics; D016131:Lymphocyte Subsets / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D014471:Umbilical Veins / Q000166:cytology; D019010:Vascular Cell Adhesion Molecule-1
chemical_list: D015815:Cell Adhesion Molecules; D016207:Cytokines; D019040:E-Selectin; D007375:Interleukin-1; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D007372:Interferons