[A method for determining the strength of the disulphide bonds of glycoproteins from gastric mucus].
The author describes a simple and highly informative method for the assessment of disulfide bonds of gastric mucus glycoproteins, based on studies of the mucus rheology before and after incubation with 5% unithiol for an hour at 37 degrees C. Such an exposure was found to induce in normal subjects just a slight reduction of viscous elastic characteristics of the mucus (under 30% of the initial values), whereas in patients with duodenal ulcer this reduction was marked: in 83 +/- 7% of patients the rheologic characteristics dropped by more than 30%, in 1/3 of the patients the mucous gel was found completely dissolved. Such an effect was observed both in the patients with the initially reduced and with normal rheologic parameters of gastric mucus.
Borisov
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D004220:Disulfides / Q000737:chemistry*; D004381:Duodenal Ulcer / Q000378:metabolism; D005260:Female; D005750:Gastric Juice / Q000737:chemistry / Q000187:drug effects; D005752:Gastric Mucins / Q000737:chemistry / Q000187:drug effects; D006801:Humans; D008297:Male; D011485:Protein Binding / Q000187:drug effects; D014494:Unithiol / Q000494:pharmacology; D014783:Viscosity
D004220:Disulfides; D005752:Gastric Mucins; D014494:Unithiol