title: The recognition of E. coli glutamine tRNA by glutaminyl-tRNA synthetase.
abstract: A variety of genetic, biochemical and structural studies have been used to determine factors ensuring the accuracy of recognition by aminoacyl-tRNA synthetases for tRNA. The identity elements of Escherichia coli tRNA(Gln) are located mainly in the anticodon and acceptor stem, and ensure the accurate recognition of the tRNA by glutaminyl-tRNA synthetase. We summarize a number of experimental techniques to define the accuracy of aminoacylation in vivo and in vitro.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
authors.forename: M J
authors.initials: MJ
authors.lastname: Rogers
authors.forename: I
authors.initials: I
authors.lastname: Weygand-Durasević
authors.forename: E
authors.initials: E
authors.lastname: Schwob
authors.forename: J M
authors.initials: JM
authors.lastname: Sherman
authors.forename: K C
authors.initials: KC
authors.lastname: Rogers
authors.forename: H U
authors.initials: HU
authors.lastname: Thomann
authors.forename: L A
authors.initials: LA
authors.lastname: Sylvers
authors.forename: E
authors.initials: E
authors.lastname: Ohtsuka
authors.forename: H
authors.initials: H
authors.lastname: Inokuchi
authors.forename: D
authors.initials: D
authors.lastname: Söll
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000926:Anticodon; D001483:Base Sequence; D004252:DNA Mutational Analysis; D004926:Escherichia coli / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism*; D012352:RNA, Transfer, Glu / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000926:Anticodon; D012329:RNA, Bacterial; D012352:RNA, Transfer, Glu; D000604:Amino Acyl-tRNA Synthetases; C020659:glutaminyl-tRNA synthetase