[The determination of the antigenic activity of recombinant virus-specific polypeptides from the herpes simplex virus types 1 and 2 and their use in immunoenzyme analysis].
The structural and nonstructural recombinant proteins of HSV type 1 and type 2 were expressed in E. coli by fusion with cro-beta-galactosidase proteins. These proteins containing amino acid sequences of ICP4, ICP27, ICP47, ICP22, major capsid protein and major DNA-binding proteins of HSV types 1 and 2 reacted in immunoblotting with the corresponding anti-HSV sera from hyperimmune rabbits. The nonstructural recombinant proteins can be used for enzyme immunoassay detection of HSV1 or HSV2 type-specific antibodies in sera from patients with acute HSV infection.
Zvonarev
A Iu
AIu
Klichko
V I
VI
Shatalin
K Iu
KIu
Kuliakina
M N
MN
Karaseva
E V
EV
D000208:Acute Disease; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis*; D015704:CD4 Antigens / Q000494:pharmacology*; D003937:Diagnosis, Differential; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000032:analysis*; D006561:Herpes Simplex / Q000175:diagnosis; D018259:Herpesvirus 1, Human / Q000276:immunology*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D010455:Peptides / Q000032:analysis*; D011994:Recombinant Proteins / Q000032:analysis
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015704:CD4 Antigens; D000939:Epitopes; D010455:Peptides; D011994:Recombinant Proteins