Anti-CD36 antibodies in patients with lupus anticoagulant and thrombotic complications.
Six patients with lupus anticoagulant with thrombotic complications, but not exhibiting systemic lupus erythematosus, demonstrated the presence in their plasma of antibodies directed against platelet antigens which were not detectable in two patients presenting with lupus anticoagulant but without thrombotic complications. Protein blotting of separated normal platelet proteins against patient plasma gave up to 18 bands of varying intensity indicative of multiple antiplatelet antibodies; one of these antibodies recognized a component with a mobility identical with CD36 (GPIV; m.w. 88,000) in 4/6 cases. Antibodies to CD36 and one or two other components were identified in 5/6 cases by immunoprecipitation from 125I-labelled control platelets and 6/6 by dot blots against purified CD36. These results suggest that antiplatelet antibodies and, specifically, anti CD36 antibodies, occur frequently in the plasma of patients presenting with lupus anticoagulant and thrombotic complications.
Rock
G
G
Department of Vascular Surgery, Ottawa Civic Hospital, Ontario, Canada.
Chauhan
K
K
Jamieson
G A
GA
Tandon
N N
NN
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D018955:CD36 Antigens; D002648:Child; D005260:Female; D006801:Humans; D015151:Immunoblotting; D016682:Lupus Coagulation Inhibitor / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D010980:Platelet Membrane Glycoproteins / Q000276:immunology*; D011233:Precipitin Tests
D015703:Antigens, CD; D001323:Autoantibodies; D018955:CD36 Antigens; D016682:Lupus Coagulation Inhibitor; D010980:Platelet Membrane Glycoproteins