[On the localization of the binding site(s) of antibodies against TSH receptor using synthetic peptides].
In order to elucidate autoimmune B-cell recognition sites of human thyrotropin receptor (TSHR), nine overlapping synthetic peptides (residues 12-30, 24-44, 151-175, 171-195, 308-328, 324-344, 339-364, 359-380, and 375-399) were synthesized and biological activities of antibodies against each of the peptide were examined. It was shown that antibodies against TSHR 12-30, 24-44, 151-175, 171-195, 324-344, and 359-380 had thyroid stimulating antibody activity (TSAb), whereas antibodies against 339-364, 359-380, and 375-399 had thyroid stimulation blocking activity (TSBAb). From these results it was concluded that epitopes where TSH receptor antibodies with TSAb and TSBAb activities are bound are widely located in N-terminal, mid portion, and C-terminal regions.
Sakata
S
S
Third Department of Internal Medicine, Gifu University School of Medicine.
Matsui
K
K
Sarui
H
H
Takuno
H
H
Yasuda
K
K
D000818:Animals; D001323:Autoantibodies / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D001666:Binding Sites, Antibody; D000939:Epitopes; D006801:Humans; D018828:Immunoglobulins, Thyroid-Stimulating; D010455:Peptides; D011989:Receptors, Thyrotropin / Q000276:immunology*
D001323:Autoantibodies; D000939:Epitopes; D018828:Immunoglobulins, Thyroid-Stimulating; D010455:Peptides; D011989:Receptors, Thyrotropin