Characterization of homogeneously staining regions in a small cell lung cancer cell line, using in situ hybridization with an MYCN probe.
The cell line CIPL38 was derived from the pleural effusion of a patient with small cell lung cancer. The karyotype was hyperdiploid and complex with a variable number of marker chromosomes. Two of the markers had large homogeneously staining regions (hsr), which were shown to consist of amplified MYCN by in situ hybridization. One hsr bearing a marker chromosome could not be identified with G-banding, but the other was situated on a der(14). This was elucidated further with FISH analysis, which enabled the identification of sequences of chromosome i involved in a complex rearrangement with chromosome 14 and the hsr.
Dietzsch
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University of Melbourne Department of Medicine, Australia.
Lukeis
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RE
Vrazas
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Hasthorpe
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Garson
O M
OM
D000368:Aged; D015139:Blotting, Southern; D018288:Carcinoma, Small Cell / Q000235:genetics*; D002871:Chromosome Banding; D005260:Female; D016259:Genes, myc; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics*; D015345:Oligonucleotide Probes; D014407:Tumor Cells, Cultured
D015345:Oligonucleotide Probes