Genotoxicity evaluation of pyrazinamide in mice.
Pyrazinamide, an antituberculosis drug, was investigated for genotoxicity in mice, an in vivo rodent system. Three doses (125, 250 and 500 mg/kg bw corresponding to 5, 10 and 20 times the therapeutic dose respectively) were tested. The mitotic index and the frequency of chromosomal aberrations were analysed at three sample times (3, 6 and 24 h) after a single intraperitoneal treatment. The frequency of sperm shape abnormalities was also examined. The mitotic index showed a decrease in drug-treated animals compared with that recorded in the control set. The cells with chromosomal aberrations ranged from 4% to 8%. The maximum frequency of aberrations was found at the 3-h sample time. The frequency of sperm shape abnormalities showed a dose-related increase. These observations suggest pyrazinamide to be a weak genotoxicant at the doses tested.
Anitha
B
B
Department of Zoology, University of Madras, India.
Sudha
S
S
Gopinath
P M
PM
Durairaj
G
G
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D008940:Mitotic Index; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D011718:Pyrazinamide / Q000633:toxicity*; D012044:Regression Analysis; D016196:S Phase / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*
D009153:Mutagens; D011718:Pyrazinamide