Commonly used cat reporter vectors contain a cAMP-inducible, cryptic enhancer that co-operates with NF-kappa B-sites.
Commercially available and widely used cat expression vectors were found to contain a forskolin (Fs)-inducible element capable of co-operation with NF-kappa B-sites in test promoters. An alternative NF-kappa B-dependent reporter system is presented that allows investigation of the effects of Fs and other agents that augment intracellular cyclic AMP.
Ghersa
P
P
GLAXO Institute for Molecular Biology, Geneva, Switzerland.
Whelan
J
J
Pescini
R
R
DeLamarter
J F
JF
Hooft van Huijsduijnen
R
R
D001665:Binding Sites; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics*; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis* / Q000235:genetics; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D019040:E-Selectin; D004727:Endothelium / Q000378:metabolism*; D004742:Enhancer Elements, Genetic; D004790:Enzyme Induction; D005822:Genetic Vectors; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic; D014471:Umbilical Veins
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D016328:NF-kappa B; D005576:Colforsin; D000242:Cyclic AMP; D015500:Chloramphenicol O-Acetyltransferase; D017868:Cyclic AMP-Dependent Protein Kinases