title: Urinary alpha-amylase and serum macroamylase activities in dogs with proteinuria.
abstract: Activities of urinary alpha-amylase and serum macroamylase; concentrations of serum creatinine, immunocomplexes, and urinary protein; and patterns of proteinuria were determined in 35 dogs with proteinuria. Urinary alpha-amylase activity ranged from 37 to 4,031 U/L. Macroamylasemia was detected in 77.14% of dogs and the percentage of alpha-amylase precipitated ranged from 4.68 to 61.63. Serum alpha-amylase activity after immunoglobulin precipitation ranged from 654 to 6,390 U/L in 51.42% of the dogs; the values were higher than the reference limits. Concentrations of serum creatinine and immunocomplexes were higher than reference limits for 25.71 and 60% of dogs, respectively. Urinary protein concentrations ranged from 0.1 to 8.9 g/L. All the patterns of proteinuria were represented. Linear regression indicated correlations between urinary alpha-amylase activities, serum creatinine concentrations (P < 0.01), and concentration of immunocomplexes (P < 0.05). Mann-Whitney test indicated significantly higher urinary alpha-amylase activity (P < 0.01) and percentage of alpha-amylase precipitated (P < 0.05) in dogs with renal insufficiency.
authors.affiliation: Facolta di Medicina Veterinaria, Istituto di Patologia, Speciale e Clinica Medica, Pisa, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Corazza
authors.forename: R
authors.initials: R
authors.lastname: Tognetti
authors.forename: G
authors.initials: G
authors.lastname: Guidi
authors.forename: A
authors.initials: A
authors.lastname: Buonaccorsi
mesh_terms: D000681:Amylases / Q000097:blood*; D000818:Animals; D000936:Antigen-Antibody Complex / Q000097:blood; D003404:Creatinine / Q000097:blood; D004283:Dog Diseases / Q000201:enzymology*; D004285:Dogs; D005260:Female; D016014:Linear Models; D046911:Macromolecular Substances; D008297:Male; D011507:Proteinuria / Q000201:enzymology / Q000662:veterinary*; D000516:alpha-Amylases / Q000097:blood / Q000652:urine*
chemical_list: D000936:Antigen-Antibody Complex; D046911:Macromolecular Substances; D003404:Creatinine; D000681:Amylases; C001663:macroamylase; D000516:alpha-Amylases