Detection of specific antibodies in human blastomycosis by enzyme immunoassay.
Serologic tests for the diagnosis of blastomycosis have had inadequate sensitivity, but results are improved with enzyme immunoassay (EIA) with A-antigen of Blastomyces dermatitidis. The Premier Blastomyces EIA kit (Meridian Diagnostics Inc, Cincinnati, Ohio) detected antibody against the B dermatitidis A-antigen in sera from 104 of 125 patients (83%) with culture-proven blastomycosis. Semiquantitative index values (positivity multiplied by the dilution) ranged from 1.0 to 423.3; the degree of positivity correlated with disease activity. Immunodiffusion bands were infrequently detected, with positive specimens from only 19 (21%) of 92 patients with blastomycosis. In 8 of 24 patients with histoplasmosis, 24 of 51 specimens (47%) were positive for Blastomyces antibody by EIA; index values were lower, with a mean of 3.8 versus 24.3 for sera from patients with blastomycosis. Only 1 of 13 patients with other fungal infections had positive results with EIA. This EIA system provided better sensitivity than previously available commercial systems for serodiagnosis of blastomycosis.
Bradsher
R W
RW
Department of Medicine, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Pappas
P G
PG
D000908:Antibodies, Fungal / Q000302:isolation & purification*; D001758:Blastomyces / Q000276:immunology*; D001759:Blastomycosis / Q000175:diagnosis*; D003429:Cross Reactions; D006801:Humans; D007124:Immunoenzyme Techniques; D012680:Sensitivity and Specificity
D000908:Antibodies, Fungal