title: Single channel properties of the 5-HT3 subtype of serotonin receptor in primary cultures of rodent hippocampus.
abstract: Whole cell and outside-out patch clamp recordings were obtained from primary cultures of rat and mouse hippocampal neurons. Serotonin (5-HT) evoked short-latency fast inward currents in about 5% of neurons. These currents reversed near 0 mV, showed inward rectification, and were inhibited by the 5-HT3 receptor antagonists ICS 205-930, ondansetron and tubocurare. 5-HT activated single channel currents in 14 of 24 membrane patches obtained from neurons which showed 5-HT3-activated whole cell currents; mean amplitude of channel openings was 0.95 +/- 0.09 pA at -100 mV. Chord conductances measured at -80 and -160 mV were 8.3 and 10.5 pS, respectively. 5-HT-induced unitary currents were blocked reversibly by tubocurare (1 microM), ICS 205-930 (30 nM) and ondansetron (100 nM). Thus, single-channel properties of 5-HT3 receptors in hippocampal neurons are similar to those present in peripheral neurons and do not exhibit solely the sub-picosiemen conductance characteristic of the neuroblastoma and neuroblastoma-derived cloned 5-HT3 receptor.
authors.affiliation: Glaxo Institute for Molecular Biology, Geneva, Switzerland.
authors.forename: K A
authors.initials: KA
authors.lastname: Jones
authors.forename: A
authors.initials: A
authors.lastname: Surprenant
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015640:Ion Channel Gating / Q000187:drug effects; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D051379:Mice; D018408:Patch-Clamp Techniques; D051381:Rats; D011985:Receptors, Serotonin / Q000187:drug effects / Q000378:metabolism*; D012701:Serotonin / Q000494:pharmacology; D012702:Serotonin Antagonists / Q000494:pharmacology
chemical_list: D007473:Ion Channels; D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D012701:Serotonin