Interferon caused alterations of human cell response to bleomycin in vitro.
HeLa cells were exposed to recombinant interferon-alpha (rIFN-alpha) in combination with bleomycin (BLM) concentrations known to exert single as well as double-strand breakages of cellular DNA. rIFN-alpha is added before or/and after cell treatment with BLM. The sensitivity of HeLa cells to BLM or/and rIFN was assessed from the number and the size of cell colonies formed. rIFN-alpha alone at concentrations of 250 IU/ml had no effect on the development of HeLa cell colonies, in combination with BLM, rIFN-alpha increased the already marked inhibition of HeLa cell colony formation caused by 1 microgram/ml BLM. At BLM concentrations of 5 and 10 micrograms/ml the inhibition of HeLa cell colony formation was almost complete and no overlapped effect of BLM and rIFN-alpha can be distinguished. However, rIFN-alpha afforded protection of HeLa cells colony formation when it was added before BLM treatment or it was present continuously thereafter.
Giannoylaki
H
H
Institute of Biology, National Centre for Scientific Research Demokritos, Athens, Greece.
Havredaki
M
M
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000494:pharmacology*; D001761:Bleomycin / Q000008:administration & dosage; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I / Q000008:administration & dosage; D011994:Recombinant Proteins
D007370:Interferon Type I; D011994:Recombinant Proteins; D001761:Bleomycin