title: Quantization of CD34+ peripheral blood hematopoietic progenitors for autografting in cancer patients.
abstract: After myeloablative regimens, combined reinfusion of peripheral blood hematopoietic circulating progenitor cells (CPC) and bone marrow, yields a very rapid hematopoietic recovery. Therefore, based on the knowledge that CPC express the CD34 and CD33 differentiation antigen, we have developed a direct immunofluorescence flow cytometry assay to detect the peak of CPC in the peripheral blood of patients treated with high dose chemotherapy and growth factors. This assay, compared to CFU-GM assay, has the following advantages: 1) easy to do 2) standardized method 3) real time information on CPC number. This work illustrates the practical aspects of this assay and substantiate the widespread use of the CD34/33 flow cytometry assay to guide harvesting of circulating hematopoietic progenitors for autologous transplantation.
authors.affiliation: Cristina Gandini Unit, Istituto Nazionale Tumori, Milano, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Di Nicola
authors.forename: S
authors.initials: S
authors.lastname: Siena
authors.forename: M
authors.initials: M
authors.lastname: Bregni
authors.forename: F
authors.initials: F
authors.lastname: Peccatori
authors.forename: M
authors.initials: M
authors.lastname: Magni
authors.forename: F
authors.initials: F
authors.lastname: Ravagnani
authors.forename: L
authors.initials: L
authors.lastname: Zorzino
authors.forename: G
authors.initials: G
authors.lastname: Bonadonna
authors.forename: A M
authors.initials: AM
authors.lastname: Gianni
mesh_terms: D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D000970:Antineoplastic Agents / Q000627:therapeutic use; D003114:Colony-Forming Units Assay; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D007937:Leukapheresis; D009369:Neoplasms / Q000628:therapy*; D014182:Transplantation, Autologous
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D000970:Antineoplastic Agents