Expression of the Alcaligenes eutrophus phbA gene in Escherichia coli using a positive selection vector based on phage Lambda lysis genes.
A new positive selection vector, pGS23, based on the Lambda lysis cassette has been designed for efficient expression of homologous and heterologous genes in Escherichia coli. The plasmid permits controlled expression of a gene of interest under transcriptional control of the lac promoter with translation initiation of coding sequences directed by the phage T7 gene 10 ribosome binding site. The application of the vector system was tested for high level expression of the heterologous phbA gene of Alcaligenes eutrophus in E. coli.
Kalousek
S
S
Institute of Microbiology and Genetics, University of Vienna, Austria.
Schrot
G
G
Lubitz
W
W
Bläsi
U
U
D000102:Acetyl-CoA C-Acyltransferase / Q000235:genetics; D000421:Alcaligenes / Q000201:enzymology / Q000235:genetics*; D010582:Bacteriophage lambda / Q000235:genetics; D001483:Base Sequence; D001709:Biotechnology; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D005814:Genes, Viral; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D000102:Acetyl-CoA C-Acyltransferase