Transient immunohistochemical labelling of rat retinal axons during Wallerian degeneration by a monoclonal antibody to neurofilaments.
Immunohistochemical labelling with the monoclonal antibody SMI32 to non-phosphorylated epitopes on neurofilament proteins of high molecular weight class was low in rat central optic fibers of controls. After unilateral transection of optic nerve, a strong, transient increase of labelling with SMI32 occurred in degenerating fibers of optic tract at 2 and 4 days, which then declined at 8 and remained low at 21 days. Consequently, immunostaining with SMI32 may serve as a positive marker for degenerating fibers in rat optic system.
Meller
D
D
Department of Neurophysiology, Medical Faculty, Ruhr-Universität Bochum, Germany.
Eysel
U T
UT
Schmidt-Kastner
R
R
D000818:Animals; D000911:Antibodies, Monoclonal; D001369:Axons / Q000378:metabolism* / Q000502:physiology / Q000648:ultrastructure; D000939:Epitopes / Q000276:immunology; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D016900:Neurofilament Proteins / Q000276:immunology*; D009900:Optic Nerve / Q000502:physiology; D051381:Rats; D012160:Retina / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D014855:Wallerian Degeneration / Q000187:drug effects / Q000502:physiology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D016900:Neurofilament Proteins