In vitro and in vivo genotoxicity evaluation of hormonal drugs v. mestranol.
Genotoxicity of a widely used estrogen, Mestranol, was undertaken using in vitro, in vivo and host-mediated assay with bacteria as indicator organism. Analyses of chromosome aberrations and sister chromatid exchanges (SCEs) in human lymphocytes and chromosome aberrations, micronuclei and sister chromatid exchanges (SCEs) in bone-marrow cells of mice showed the drug to be capable of attacking the genetic material. However, both Ames Salmonella/S9 assay with and without S9 mix and host-mediated assay using same tester strains of Salmonella, did not show any significant increase/decrease in the His+ revertants.
Dhillon
V S
VS
Department of Human Genetics, Guru Nanak Dev University, Amritsar, India.
Singh
J R
JR
Singh
H
H
Kler
R S
RS
D000818:Animals; D001853:Bone Marrow / Q000648:ultrastructure; D002869:Chromosome Aberrations; D006801:Humans; D008297:Male; D008656:Mestranol / Q000633:toxicity*; D051379:Mice; D015162:Micronucleus Tests; D009152:Mutagenicity Tests / Q000379:methods; D009153:Mutagens; D012486:Salmonella typhimurium / Q000235:genetics; D012854:Sister Chromatid Exchange
D009153:Mutagens; D008656:Mestranol