Effect of hydrogen peroxide and catalase on rat cerebellum nitric oxide synthase.
The effect of H2O2 and catalase on isolated rat cerebellum nitric oxide (NO) synthase activity was determined by measuring the conversion of L-[3H]arginine to L-[3H]citrulline. H2O2 (1-5 mM) markedly increased NO synthase activity in the presence of endogenous catalase (72 +/- 4 U/mL). This effect of H2O2 was further increased by exogenous catalase (200 U/mL). Exogenous catalase (0.1 to 1000 U/mL) by itself had no significant effect on NO synthase activity. Nitroblue tetrazolium chloride, an electron acceptor, inhibited NO synthase activity in a concentration-dependent manner. This study suggests that H2O2 is not directly involved in NO synthesis and that the H2O2/catalase stimulation of NO synthase activity may be due to the excess oxygen produced by the H2O2/catalase system.
Rengasamy
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A
Department of Anesthesiology, University of Virginia Health Sciences Center, Charlottesville 22908.
Johns
R A
RA
D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000378:metabolism; D002374:Catalase / Q000494:pharmacology*; D002531:Cerebellum / Q000187:drug effects* / Q000201:enzymology; D002956:Citrulline / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D066298:In Vitro Techniques; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase; D051381:Rats; D013778:Tetrazolium Salts / Q000494:pharmacology
D013778:Tetrazolium Salts; C042207:4-anisyltetrazolium blue; D002956:Citrulline; D009569:Nitric Oxide; D001120:Arginine; D006861:Hydrogen Peroxide; D002374:Catalase; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases