Immunofluorescence analysis of B-1 cell ontogeny in the mouse.
In order to further understand the developmental aspects of B-1 cells, we characterized the ontogeny of this B cell population in the spleen and peritoneal cavity of BALB/c mice. Although there are B-1 cells in the spleen within the first 1-3 weeks after birth, they do not at any stage represent the majority of splenic B cells. Splenic B-1 cells reach peak levels at approximately 9 days after birth. The mesenteric lining that covers the small intestine of 7-day-old mice contains a population of IgM+ B cells, while at the same age, there are few lymphoid cells in the peritoneal cavity. Between 7 and 8 days after birth there is an influx of B cells into the peritoneal cavity. At 8 days, the first detectable peritoneal B cells appear to be of the B-1 type based on expression of IL-5 receptor and CD5. However, these peritoneal B-1 cells do not express Mac-1. This antigen is not expressed by the majority of peritoneal B-1 cells until 3 weeks. This study indicates that the majority of early splenic B cells are not B-1 cells and it suggests that the mesenteric tissues surrounding the gut contain B lymphocytes which traffic into the peritoneal cavity where they then reside.
Hamilton
A M
AM
Department of Microbiology, University of Alabama at Birmingham 35294.
Lehuen
A
A
Kearney
J F
JF
D000375:Aging / Q000276:immunology; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000096:biosynthesis; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D018956:CD5 Antigens; D005455:Fluorescent Antibody Technique; D007075:Immunoglobulin M / Q000032:analysis; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D008643:Mesentery / Q000166:cytology* / Q000254:growth & development; D051379:Mice; D008807:Mice, Inbred BALB C; D010529:Peritoneal Cavity / Q000166:cytology* / Q000254:growth & development; D010533:Peritoneal Lavage; D018123:Receptors, Interleukin / Q000096:biosynthesis; D053649:Receptors, Interleukin-5; D013154:Spleen / Q000166:cytology* / Q000254:growth & development
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018956:CD5 Antigens; D007075:Immunoglobulin M; D016177:Macrophage-1 Antigen; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5