title: Epithelial localization of insulin-like growth factor binding protein 1 in the uterus of the rat during pregnancy, deciduoma-bearing pseudopregnancy and hormone treatment.
abstract: A monospecific antibody was used to determine the immunocytochemical localization of insulin-like growth factor binding protein 1 (IGFBP-1) in the rat uterus. Immunoreactive IGFBP-1 was first detected from day 5 of pregnancy in the luminal and glandular epithelium. However, immunoreactivity was most intense from day 6 in the glandular epithelium, where it was associated with apically located granules. Immunoreactive glands were located only in non-decidualized endometrium, which was limited at the implant site to a thin basal layer by growth of the antimesometrial decidua from day 7. However, glands and associated immunoreactive IGFBP-1 were prominent in the inter-implant sites until day 9, although they were detected throughout pregnancy. Similar reactivity was detected in the glands of the basal endometrium in deciduomata-bearing animals, but these decreased in number from day 7 of pseudopregnancy. No immunoreactivity was detected during the oestrous cycle but could be induced in ovariectomized animals by sequential oestradiol and oestradiol plus progesterone treatment. The observations were consistent with IGFBP-1 representing a secretory product of the glandular epithelium and could either play a role in development of the trophoblastic component of the conceptus during the pre-placental period of anti-mesometrial implantation or in the endometrium acting as an inhibitor of local IGF-I action and in either case by transporting IGF-I from the stromal to the glandular luminal environment.
authors.affiliation: Department of Obstetrics and Gynaecology, Medical School, University of Leicester, UK.
authors.forename: S
authors.initials: S
authors.lastname: Sadek
authors.forename: T G
authors.initials: TG
authors.lastname: Unterman
authors.forename: S C
authors.initials: SC
authors.lastname: Bell
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000032:analysis*; D003656:Decidua / Q000737:chemistry*; D004717:Endometrium / Q000737:chemistry; D004848:Epithelium / Q000737:chemistry / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology; D004971:Estrus / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D018970:Insulin-Like Growth Factor Binding Protein 1; D010052:Ovariectomy; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D011555:Pseudopregnancy / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013002:Somatomedins / Q000032:analysis*; D014599:Uterus / Q000737:chemistry*
chemical_list: D002352:Carrier Proteins; D018970:Insulin-Like Growth Factor Binding Protein 1; D013002:Somatomedins; D011374:Progesterone; D004958:Estradiol