title: Porcine leukocyte interferon exhibits close antigenic relatedness to human interferon alpha 2, but not to human interferon alpha 1.
abstract: As reported by others using polyclonal antisera, natural human and porcine interferons (IFN)-alpha are antigenically related. Using monoclonal antibodies (mAb) in neutralization and ELISA experiments, we found differences in the subtype/antigenic composition between virus-induced porcine and human leukocyte preparations. Human leukocyte IFN-alpha contains two major antigenically distinct subtypes, IFN-alpha 1 and IFN-alpha 2. However, swine leukocytes produced only a single predominant species of IFN-alpha with high antigenic homology to human IFN-alpha 2. Moreover, we were unable to detect close antigenic relatedness between recombinant porcine and human IFN-alpha 1 subtypes.
authors.affiliation: Institute of Virology, Slovak Academy of Sciences, Bratislava.
authors.forename: G
authors.initials: G
authors.lastname: Karayianni-Vasconcelos
authors.forename: L
authors.initials: L
authors.lastname: Borecký
authors.forename: P
authors.initials: P
authors.lastname: Kontsek
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002417:Cattle; D002460:Cell Line; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007369:Interferon Inducers; D007370:Interferon Type I / Q000276:immunology; D016898:Interferon-alpha / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D009522:Newcastle disease virus; D011994:Recombinant Proteins; D013552:Swine / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007369:Interferon Inducers; D007370:Interferon Type I; D016898:Interferon-alpha; D011994:Recombinant Proteins