title: 31P NMR investigation of energy metabolism in perifused MMQ cells.
abstract: The MMQ cell line is a unique prolactin-secreting rat pituitary cell line. MMQ cells entrapped in agarose gel threads are metabolically active, as determined by the uptake and phosphorylation of creatine and the maintenance of high energy phosphates for over 15 h. Forskolin activates the catalytic subunit of adenylyl cyclase and, in MMQ cells, elevates the level of cAMP and stimulates prolactin secretion. 31P NMR spectroscopy was used to investigate the energy metabolism of the MMQ cells during stimulation by forskolin. The ability to measure small changes in the energy status of these cells was enhanced by increasing the PCr levels in the cells. Administration of forskolin to the perifused MMQ cells resulted in acute, reversible, and dose-dependent changes in the 31P NMR spectra of the cells within 12 to 24 min of the beginning of forskolin exposure. Several lines of evidence indicate that the changes observed in the MMQ cells are the composite result of the interaction of forskolin with adenylyl cyclase and the plasma membrane glucose transporter. Also, preincubation of the MMQ cells with the dopamine agonist, bromocriptine, attenuates the forskolin-stimulated decrease in the PCr resonance by approximately 50%. This attenuation indicates that the forskolin-stimulated changes in energy metabolism are probably related to the prolactin secretion process.
authors.affiliation: Department of Chemistry, University of Virginia, Charlottesville 22901.
authors.forename: M J
authors.initials: MJ
authors.lastname: Goger
authors.forename: I S
authors.initials: IS
authors.lastname: Login
authors.forename: E J
authors.initials: EJ
authors.lastname: Fernandez
authors.forename: C M
authors.initials: CM
authors.lastname: Grisham
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology; D002460:Cell Line; D005576:Colforsin / Q000008:administration & dosage / Q000494:pharmacology*; D003401:Creatine / Q000096:biosynthesis; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects*; D004789:Enzyme Activation; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000494:pharmacology; D007473:Ion Channels / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D010759:Phosphorus Isotopes; D010902:Pituitary Gland / Q000187:drug effects* / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism; D051381:Rats; D011954:Receptors, Dopamine / Q000187:drug effects
chemical_list: D007473:Ion Channels; D010710:Phosphates; D010759:Phosphorus Isotopes; D011954:Receptors, Dopamine; D010725:Phosphocreatine; D005973:Glutamine; D005576:Colforsin; D001971:Bromocriptine; D011388:Prolactin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D005947:Glucose; D003401:Creatine