title: Monitoring of treatment outcome in acute promyelocytic leukemia by RT-PCR.
abstract: A rearrangement between the PML and RAR-alpha genes underlies the acute promyelocytic leukemia (APL)-specific t(15;17) translocation, leading to the production of a chimeric mRNA. Recent development of a reverse-transcription polymerase chain reaction (RT-PCR) assay for the PML/RAR-alpha hybrid has proven useful for rapid diagnosis and monitoring of minimal residual disease (MRD) in APL patients. Preliminary studies in which the prognostic significance of RT-PCR was evaluated indicate that this test may identify patients at high risk of relapse.
authors.affiliation: Department of Human Biopathology, University La Sapienza, Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Diverio
authors.forename: P P
authors.initials: PP
authors.lastname: Pandolfi
authors.forename: V
authors.initials: V
authors.lastname: Rossi
authors.forename: A
authors.initials: A
authors.lastname: Biondi
authors.forename: P G
authors.initials: PG
authors.lastname: Pelicci
authors.forename: F
authors.initials: F
authors.lastname: Lo Coco
mesh_terms: D006402:Hematologic Diseases / Q000235:genetics / Q000628:therapy; D006801:Humans; D007938:Leukemia / Q000235:genetics / Q000628:therapy; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics* / Q000628:therapy*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011379:Prognosis; D012194:RNA-Directed DNA Polymerase; D016896:Treatment Outcome
chemical_list: D012194:RNA-Directed DNA Polymerase