Integration site of human papillomavirus type-18 DNA in chromosome band 8q22.1 of C4-I cervical carcinoma: DNase I hypersensitivity and methylation of cellular flanking sequences.
The C4-I cell line derived from a non-keratinizing squamous cell carcinoma of the uterine cervix contains integrated human papillomavirus-18 DNA. Fluorescence in situ hybridization of C4-I cells demonstrated a single viral integration site at 8q22.1 on a derivative chromosome originating from an 8q;12q translocation. 8q22 is a site of chromosome fragility and is also recombinogenic in several human malignancies. DNase I hypersensitivity of the integration site was studied with a cellular flanking probe. A hypersensitive site was detected within 3 kb from viral DNA. The integration sites are undermethylated in C4-I and HeLa cells and fully methylated in tumor cell lines of other origin, such as lymphoid cells.
Gallego
M I
MI
Unidad de Genética Molecular, Centro Nacional de Biología Celular y Retrovirus, Instituto de Salud Carlos III, Majadahonda, Spain.
Zimonjic
D B
DB
Popescu
N C
NC
DiPaolo
J A
JA
Lazo
P A
PA
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000821:virology; D002898:Chromosomes, Human, Pair 8; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000235:genetics*; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology; D016662:Virus Integration / Q000235:genetics*
D004279:DNA, Viral