Two classes of proteins and mRNAs in Lilium longiflorum L. indentified by human vitronectin probes.
Vitronectin (VN) is a substrate adhesion molecule, an extracellular matrix glycoprotein that facilitates cell adhesion and cell movement in animals. We have reported the cross-reactivity of a 55-kD protein in plants with rabbit anti-human VN antibodies and the presence of VN-like sequences in plant genomes using a human VN cDNA probe. We have extended these studies by using human VN riboprobes to detect VN-like mRNAs in lily (Lilium longiflorum L.) and soybean. In both species, two mRNAs were detected. We have also identified a new cross-reactive protein (41 kD) using a different preparation of human VN antiserum. In lily roots five 41-kD isoforms were observed, whereas only three of these isoforms accumulated in leaves. Monospecific antibodies prepared against the plant proteins cross-reacted with the human VN protein and vice versa. We have purified the 41-kD protein using two-dimensional gel electrophoresis, and amino acid composition analysis indicates that it is similar in composition to human VN.
Wang
C S
CS
Department of Botany and Plant Sciences, University of California, Riverside 92521-0124.
Walling
L L
LL
Gu
Y Q
YQ
Ware
C F
CF
Lord
E M
EM
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies / Q000276:immunology; D003429:Cross Reactions; D015342:DNA Probes; D006023:Glycoproteins / Q000235:genetics* / Q000276:immunology; D006801:Humans; D010940:Plant Proteins / Q000032:analysis* / Q000145:classification / Q000235:genetics / Q000276:immunology; D010944:Plants; D012333:RNA, Messenger / Q000032:analysis* / Q000145:classification / Q000235:genetics; D011817:Rabbits; D013025:Glycine max; D019096:Vitronectin
D000596:Amino Acids; D000906:Antibodies; D015342:DNA Probes; D006023:Glycoproteins; D010940:Plant Proteins; D012333:RNA, Messenger; D019096:Vitronectin