title: Construction and first characterization of two reciprocal hybrids between LamB from Escherichia coli K12 and Klebsiella pneumoniae.
abstract: The LamB proteins from Klebsiella pneumoniae and Escherichia coli K12 were previously shown to be highly homologous. The most conserved parts correspond to the N-proximal third and to the transmembranous portions of the molecule, while the variability occurred essentially within regions exposed to the cell surface or to the periplasm. Since the two proteins displayed identical in vitro trimer stability and in vivo pore properties, we tested whether the N-terminal parts of the two proteins could be exchanged and still allow the formation of stable and functional maltoporins. For that purpose, we expressed the LamB protein from K. pneumoniae in E. coli K12, and constructed two reciprocal hybrids between LamB from E. coli K12 and LamB from K. pneumoniae. The first hybrid (LamBE.c.-K.p.) is composed of residues 1 to 183 from LamBE.c. followed by residues 184 to 404 from LamBK.p. The second one comprises residues 1 to 183 from LamBK.p., followed by residues 184 to 421 from LamBE.c. (LamBK.p.-E.c.). Both hybrid proteins were correctly incorporated in the outer membrane of E. coli K12. Like the two parental LamB proteins, the two hybrids could be purified by affinity chromatography on a starch-sepharose column. The LamBE.c.-K.p. hybrid formed highly stable trimers, but was strongly impaired in its in vivo maltose transport function (15% of the wild-type level). The trimers formed by LamBK.p.-E.c. hybrid were less stable, but could be detected on the surface of intact cells by four anti-LamBE.c. monoclonal antibodies. This hybrid was also affected in its in vivo maltose transport function (30% of the wild-type level). As expected from the location of the residues critical for phage adsorption, both proteins had lost the phage receptor activity of the E. coli K12 LamB protein. We also examined whether LamBE.c. could form heterotrimers with LamBK.p., LamBK.p.-E.c., and LamBE.c.-K.p. In no case were heterotrimers detected, indicating that both terminal parts of the LamB protein are involved in homotrimer formation. All these data suggest that trimer formation and activity involve rare variable residues in the conserved regions and/or variable regions.
authors.affiliation: Unité de Programmation moléculaire et Toxicologie génétique, CNRS URA1444, Institut Pasteur, Paris.
authors.forename: C
authors.initials: C
authors.lastname: Werts
authors.forename: A
authors.initials: A
authors.lastname: Charbit
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D010582:Bacteriophage lambda / Q000235:genetics; D001693:Biological Transport, Active; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D066298:In Vitro Techniques; D007711:Klebsiella pneumoniae / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008320:Maltose / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology
chemical_list: D001426:Bacterial Proteins; D000939:Epitopes; D011994:Recombinant Proteins; D008320:Maltose