title: Enhanced human IgE production results from exposure to the aromatic hydrocarbons from diesel exhaust: direct effects on B-cell IgE production.
abstract: Epidemiologic and experimental studies suggest that air pollution, and particularly diesel exhaust particles (DEPs) may play a role in the increasing prevalence and severity of airway allergic disease. We show that the extract of polyaromatic hydrocarbons (PAHs) from DEPs (PAH-DEP) enhances human IgE production from purified B cells. Interleukin-4 plus CD40 monoclonal antibody-stimulated IgE production was enhanced 20% to 360% by the addition of PAH-DEP over a period of 10 to 14 days. This effect was increased when PAH-DEP was added 2 to 5 days after cultures were initiated. PAH-DEP itself did not induce IgE production or synergize with interleukin-4 alone to induce IgE from purified B cells, suggesting that it was enhancing ongoing IgE production rather than inducing germline transcription or isotype switching. The prototype nonmetabolized aromatic hydrocarbon 2,3,7,8 tetracholorodibenzo-p-dioxin, which functions solely through activation of the cytosolic aromatic hydrocarbon receptor complex, also increased IgE production. Additionally, the pattern of mRNAs coding for distinct isoforms of the epsilon chain was altered by PAH-DEP, and B-cell expression of the low-affinity IgE receptor was upregulated by PAH-DEP. Enhanced IgE production in the human airway, resulting from exposure to PAH-DEP, may be an important factor in the increase in airway allergic disease.
authors.affiliation: Hart and Louise Lyon Laboratory, Department of Medicine, UCLA School of Medicine 90024-1680.
authors.forename: H
authors.initials: H
authors.lastname: Takenaka
authors.forename: K
authors.initials: K
authors.lastname: Zhang
authors.forename: D
authors.initials: D
authors.lastname: Diaz-Sanchez
authors.forename: A
authors.initials: A
authors.lastname: Tsien
authors.forename: A
authors.initials: A
authors.lastname: Saxon
mesh_terms: D000328:Adult; D001402:B-Lymphocytes / Q000737:chemistry / Q000187:drug effects* / Q000276:immunology; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects*; D015847:Interleukin-4 / Q000494:pharmacology; D008875:Middle Aged; D008969:Molecular Sequence Data; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology; D011083:Polycyclic Compounds / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis / Q000187:drug effects / Q000235:genetics; D013997:Time Factors; D001335:Vehicle Emissions / Q000009:adverse effects*
chemical_list: D017931:DNA Primers; D000072317:Polychlorinated Dibenzodioxins; D011083:Polycyclic Compounds; D012333:RNA, Messenger; D001335:Vehicle Emissions; D015847:Interleukin-4; D007073:Immunoglobulin E