Species and age-dependent differences of functional coupling between opioid delta-receptor and G-proteins and possible involvement of protein kinase C in striatal membranes.
[D-Ser2,Leu5]-enkephalin-Thr6 (DSLET) and [D-Pen2,5]-enkephalin (DPDPE), both delta-agonists, stimulated the high affinity GTPase in the rat striatal membranes in a naltrindole-reversible manner. Similar stimulation was also observed in the striatal membrane preparations of 16-week-old guinea pigs, while not in those preparations of 4-week-old ones. When calphostin C, a protein kinase C inhibitor, was added to the reaction mixture, DSLET showed a marked stimulation in this activity in 4-week guinea pig striatal membranes. There was no effect of KT5720, a cyclic AMP-dependent protein kinase inhibitor, on such delta-opioid-mediated responses. These findings suggest that protein kinase C is locally involved in the functional uncoupling of delta-receptors to G-proteins in the striatum of young guinea pigs.
Fukushima
N
N
Department of Pharmacology, Yokohama City University School of Medicine, Japan.
Ueda
H
H
Hayashi
C
C
Katayama
T
T
Miyamae
T
T
Misu
Y
Y
D000375:Aging / Q000378:metabolism*; D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D002227:Carbazoles; D002462:Cell Membrane / Q000378:metabolism; D003342:Corpus Striatum / Q000254:growth & development / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D020881:Enkephalin, D-Penicillamine (2,5)-; D004743:Enkephalin, Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D004745:Enkephalins / Q000494:pharmacology; D020558:GTP Phosphohydrolases / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006168:Guinea Pigs; D007211:Indoles / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D009271:Naltrexone / Q000031:analogs & derivatives / Q000494:pharmacology; D009281:Naphthalenes; D011083:Polycyclic Compounds / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011758:Pyrroles / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017465:Receptors, Opioid, delta / Q000378:metabolism*; D013045:Species Specificity
D000700:Analgesics; D002227:Carbazoles; D004745:Enkephalins; D007211:Indoles; D009281:Naphthalenes; D011083:Polycyclic Compounds; D011758:Pyrroles; D017465:Receptors, Opioid, delta; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D004743:Enkephalin, Leucine; C057416:KT 5720; D009271:Naltrexone; C034318:enkephalin, Ser(2), Leu(5), Thr(6)-; D020881:Enkephalin, D-Penicillamine (2,5)-; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; C055382:naltrindole; C058819:calphostin C