Potassium and calcium channel dependence of bursting in cultured neuronal networks.
Increases in extracellular potassium concentrations reliably increase burst rates in cultured fetal murine spinal cord networks. This effect could be mimicked by either blocking voltage-gated potassium conductances or facilitating excitatory synaptic interactions, but not by blocking specific calcium-dependent potassium conductances or tonic depolarization. Spontaneous bursting in cultured networks is apparently dependent on potassium currents and intracellular calcium levels, but not on the pharmacologically characterized calcium-dependent potassium conductances.
Rhoades
B K
BK
Center for Network Neuroscience, University of North Texas, Denton 76203.
Gross
G W
GW
D015761:4-Aminopyridine / Q000494:pharmacology; D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D001030:Apamin / Q000494:pharmacology; D017609:Barium Compounds / Q000494:pharmacology; D001640:Bicuculline / Q000031:analogs & derivatives / Q000494:pharmacology; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D002586:Cesium / Q000494:pharmacology; D018999:Charybdotoxin; D002712:Chlorides / Q000494:pharmacology; D002794:Choline / Q000494:pharmacology; D004110:Diltiazem / Q000494:pharmacology; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D005333:Fetus; D015636:Magnesium Chloride / Q000494:pharmacology; D051379:Mice; D009116:Muscarine / Q000494:pharmacology; D016202:N-Methylaspartate / Q000494:pharmacology; D009415:Nerve Net / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques; D010042:Ouabain / Q000494:pharmacology; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D011189:Potassium Chloride / Q000494:pharmacology; D012604:Scorpion Venoms / Q000494:pharmacology; D013116:Spinal Cord / Q000502:physiology*; D019789:Tetraethylammonium; D013757:Tetraethylammonium Compounds / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology
D017609:Barium Compounds; D015220:Calcium Channels; D002712:Chlorides; D015221:Potassium Channels; D012604:Scorpion Venoms; D013757:Tetraethylammonium Compounds; D015636:Magnesium Chloride; C024986:barium chloride; D018999:Charybdotoxin; D002586:Cesium; D015124:8-Bromo Cyclic Adenosine Monophosphate; D001030:Apamin; C017069:bicuculline methiodide; D010042:Ouabain; D016202:N-Methylaspartate; D019789:Tetraethylammonium; D011189:Potassium Chloride; D014701:Veratridine; D009116:Muscarine; D015761:4-Aminopyridine; D004110:Diltiazem; C028019:cesium chloride; D002794:Choline; D000109:Acetylcholine; D001640:Bicuculline