Effect of oestradiol on the carbohydrate metabolism of immature rat uterus: the role of fructose-2, 6-bis-phosphate and of phosphoribosyl pyrophosphate.
Enzymes and metabolic intermediates of glycolysis, pentose phosphate pathway and the tricarboxylic acid cycle were measured in immature rat uterus after treatment with oestradiol. The flux of glucose through alternative pathways was examined. Fructose-2,6-bis-phosphate, the well known regulator of glycolytic pathway, increased after the injection of oestradiol and remained elevated. This increase was accompanied by raised levels of most of glycolytic intermediates and by increase in glycolytic flux. The key enzymes of glycolysis and all the enzymes of pentose phosphate pathway showed a gradual increase in the activity with administration of oestradiol up to 48 hours. Phosphoribosyl pyrophosphate, the metabolite required in nucleotide synthesis, was also elevated. Marked changes in the levels of key metabolic intermediates and the enzyme activities are correlated with the increased nucleic acid, protein and lipid synthesis occurring following oestradiol treatment.
Baquer
N Z
NZ
School of Life Sciences, J. Nehru University, New Delhi, India.
Sochor
M
M
Kunjara
S
S
McLean
P
P
D000818:Animals; D002952:Citric Acid Cycle / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005635:Fructosediphosphates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects; D008055:Lipids / Q000096:biosynthesis; D009696:Nucleic Acids / Q000096:biosynthesis; D010427:Pentose Phosphate Pathway / Q000187:drug effects; D010754:Phosphoribosyl Pyrophosphate / Q000502:physiology*; D010766:Phosphorylation; D014176:Protein Biosynthesis; D051381:Rats; D014599:Uterus / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism
D005635:Fructosediphosphates; D008055:Lipids; D009696:Nucleic Acids; D004958:Estradiol; D010754:Phosphoribosyl Pyrophosphate; C027652:fructose 2,6-diphosphate; D005947:Glucose