Inhibition of interleukin 8 attenuates angiogenesis in bronchogenic carcinoma.
We investigated the role of interleukin 8 (IL-8) in mediating angiogenesis in human bronchogenic carcinoma. Increased quantities of IL-8 were detected in tumor tissue as compared with normal lung tissue. Immunohistochemical staining of tumors revealed primary localization of IL-8 to individual tumor cells and demonstrated the capacity of tumor to elaborate IL-8. Functional studies that used tissue homogenates of tumors demonstrated the induction of both in vitro endothelial cell chemotaxis and in vivo corneal neovascularization. It is important to note that the addition of neutralizing antisera to IL-8 to these assays resulted in the marked and specific attenuation of these responses. Our observations definitively establish IL-8 as a primary mediator of angiogenesis in bronchogenic carcinoma and offer a potential target for immunotherapies against solid malignancies.
Smith
D R
DR
Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109-0360.
Polverini
P J
PJ
Kunkel
S L
SL
Orringer
M B
MB
Whyte
R I
RI
Burdick
M D
MD
Wilke
C A
CA
Strieter
R M
RM
D000818:Animals; D002283:Carcinoma, Bronchogenic / Q000098:blood supply*; D002633:Chemotaxis; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000502:physiology*; D008168:Lung / Q000737:chemistry; D008175:Lung Neoplasms / Q000098:blood supply*; D009389:Neovascularization, Pathologic / Q000209:etiology*; D051381:Rats; D011916:Rats, Inbred F344
D016209:Interleukin-8