title: Lindane affects phosphoinositide turnover through a different mechanism of the phosphatidylinositol synthesis inhibition in rat renal proximal tubule cell culture.
abstract: The ability of lindane to change the metabolism of inositol phospholipids was investigated using rat renal proximal tubular cell cultures labelled with [3H]inositol. Lindane addition to the culture medium caused labelling of inositol trisphosphate, inositol bisphosphate and inositol tetrakisphosphate to decrease and that of the inositol monophosphate pool to increase. A depletion of radioactivity in phosphatidylinositols was also observed after lindane addition. Most strikingly, the addition of lindane considerably increased the levels of glycerophosphoinositol in a dose-dependent manner. The effect of lindane follows a different pattern from that of bradykinin, and it is suggested to act by stimulating phospholipase A activity(ies).
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, Madrid, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Senar
authors.forename: M N
authors.initials: MN
authors.lastname: Recio
authors.forename: M A
authors.initials: MA
authors.lastname: Pérez-Albarsanz
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D001556:Hexachlorocyclohexane / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000096:biosynthesis* / Q000378:metabolism*; D051381:Rats
chemical_list: D003470:Culture Media; D010716:Phosphatidylinositols; D001556:Hexachlorocyclohexane