Deletions induced in the white and vermilion genes of Drosophila melanogaster by the antitumor drug cis-dichlorodiammineplatinum(II).
This paper describes the analysis of cisplatin induced mutations at the white (w) and vermilion (v) loci located on the X chromosome of Drosophila melanogaster. Twenty-eight w and eight v mutants have been found in a male genetic context and 42 w mutants in a female genetic context. In these latter experiments, genetic analysis showed the presence of multi-locus deficiencies in 18 out of 42 w mutants. Eighteen w and three v intragenic mutations were analyzed at the molecular level. Seventeen w and three v mutants carry deletions within the gene, ranging in size from 4 to 109 base pairs. Sequence analysis of the mutants indicates that most of them were produced by non-homologous recombinational events occurring between short (2-5 bp) sequence repeats on both sides of the deletion, one repeat being retained at the new junction. These results differ largely from those obtained in prokaryotic and other eukaryotic cells.
Cizeau
J
J
CBM CNRS, Orléans, France.
Decoville
M
M
Leng
M
M
Locker
D
D
D018528:ATP-Binding Cassette Transporters; D000818:Animals; D001483:Base Sequence; D002945:Cisplatin / Q000494:pharmacology*; D017931:DNA Primers; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005136:Eye Proteins; D005260:Female; D017353:Gene Deletion; D017344:Genes, Insect; D007301:Insect Hormones / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D014960:X Chromosome
D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D029721:Drosophila Proteins; D005136:Eye Proteins; D007301:Insect Hormones; C059254:w protein, Drosophila; D002945:Cisplatin