title: Ctk: a protein-tyrosine kinase related to Csk that defines an enzyme family.
abstract: We used the polymerase chain reaction with degenerate oligonucleotide primers to search for Csk-related kinases. A cDNA coding for a Csk-like protein-tyrosine kinase was cloned from mouse brain and was designated ctk, for csk-type protein-tyrosine kinase. The 1.9-kb ctk mRNA was found to be expressed predominantly in brain and capable of encoding a 52-kDa protein-tyrosine kinase. The amino acid sequence of Ctk was found to possess 53% identity with mouse Csk, shared all the predicted structural features of Csk, and was capable of phosphorylating the carboxyl-terminal conserved tyrosine of Src family members. Our results thereby indicate that ctk represents a gene that defines a family of structurally and functionally related Csk-like protein-tyrosine kinases.
authors.affiliation: Department of Molecular Biology, Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543.
authors.forename: S
authors.initials: S
authors.lastname: Klages
authors.forename: D
authors.initials: D
authors.lastname: Adam
authors.forename: K
authors.initials: K
authors.lastname: Class
authors.forename: J
authors.initials: J
authors.lastname: Fargnoli
authors.forename: J B
authors.initials: JB
authors.lastname: Bolen
authors.forename: R C
authors.initials: RC
authors.lastname: Penhallow
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D000081247:CSK Tyrosine-Protein Kinase; D003001:Cloning, Molecular; D051379:Mice; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000145:classification* / Q000235:genetics*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014018:Tissue Distribution; D019061:src-Family Kinases
chemical_list: C086603:Matk protein, mouse; D011505:Protein-Tyrosine Kinases; D000081247:CSK Tyrosine-Protein Kinase; D016392:Proto-Oncogene Proteins pp60(c-src); D019061:src-Family Kinases