Inducible nitric oxide synthase in human lymphomononuclear cells activated by synthetic peptides derived from extracellular matrix proteins.
Synthetic peptides with sequences present in extracellular matrix proteins are capable of causing the expression of the inducible form of nitric oxide synthase (iNOS), detected by immunocytochemistry, and the release of NO by human lymphomononuclear cells incubated in their presence. Active peptides are 15-mers containing a characteristic 2-6-11 motif in which the amino acid residue at position 2 is Leu, Ile, Val, Gly, Ala or Lys; the residue at position 6 is always Pro; and residue 11 is Glu or Asp. The induction of iNOS in human monocytes and macrophages could be involved in the cytotoxicity against tumor cell lines also elicited by these peptides.
Pérez-Mediavilla
L A
LA
Department of Biochemistry, University of Navarra, Pamplona, Spain.
López-Zabalza
M J
MJ
Calonge
M
M
Montuenga
L
L
López-Moratalla
N
N
Santiago
E
E
D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis*; D000595:Amino Acid Sequence; D002478:Cells, Cultured; D004790:Enzyme Induction; D016326:Extracellular Matrix Proteins / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000502:physiology; D013205:Staphylococcal Protein A / Q000494:pharmacology
D016326:Extracellular Matrix Proteins; D010446:Peptide Fragments; D013205:Staphylococcal Protein A; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases