Expression of an outwardly rectifying K+ channel in rat microglia cultivated on teflon.
Membrane currents of cultured human monocytes and rat microglia were recorded with the whole-cell patch clamp technique. Freshly isolated monocytes or resting (proliferating) microglia express only inwardly rectifying K+ channels. However, incubation in teflon bags leads to the expression of additional, outwardly rectifying K+ channels. The outward K+ conductance of microglial cells was inhibited by intracellular Cs+ and extracellular 4-aminopyridine or tetraethylammonium. Functional similarities with the microglial outwardly rectifying K+ channel were found in the Kn-channel of lymphocytes which has recently been cloned (RGK5). The polymerase chain reaction (PCR) was used to demonstrate the presence of RGK5-like mRNA in microglia.
Nörenberg
W
W
Department of Pharmacology, University of Freiburg, FRG.
Appel
K
K
Bauer
J
J
Gebicke-Haerter
P J
PJ
Illes
P
P
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000378:metabolism; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D017628:Microglia / Q000378:metabolism*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000378:metabolism; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011138:Polytetrafluoroethylene; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism*; D012313:RNA / Q000032:analysis / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar
D018076:DNA, Complementary; D008070:Lipopolysaccharides; D015345:Oligonucleotide Probes; D015221:Potassium Channels; D012313:RNA; D011138:Polytetrafluoroethylene