title: Use of exfoliative cytology in the diagnosis of oral hairy leukoplakia.
abstract: The possibility of diagnosing oral hairy leukoplakia by means of exfoliative cytology and the Papanicolaou stain was investigated. Exfoliative cytology and punch biopsy specimens were obtained from 10 lesions that demonstrated clinical features of hairy leukoplakia. All biopsy specimens demonstrated the characteristic histopathologic features of hairy leukoplakia whereas all Papanicolaou-stained cytologic smears demonstrated condensation and margination of the nuclear chromatin (nuclear beading). All biopsy specimens and cytologic smears displayed positive Epstein-Barr virus deoxyribonucleic acid in situ hybridization. We conclude that routine exfoliative cytology may be a reliable, noninvasive, and inexpensive technique for the diagnosis of hairy leukoplakia.
authors.affiliation: Department of Oral Diagnostic Sciences, University of Florida College of Dentistry.
authors.forename: C A
authors.initials: CA
authors.lastname: Migliorati
authors.forename: A C
authors.initials: AC
authors.lastname: Jones
authors.forename: P A
authors.initials: PA
authors.lastname: Baughman
mesh_terms: D000328:Adult; D001706:Biopsy; D002843:Chromatin / Q000473:pathology; D003581:Cytodiagnosis / Q000379:methods; D004279:DNA, Viral / Q000032:analysis; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D017733:Leukoplakia, Hairy / Q000175:diagnosis* / Q000382:microbiology / Q000473:pathology; D008297:Male; D014779:Virus Replication
chemical_list: D002843:Chromatin; D004279:DNA, Viral; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin