In vitro translation of fractionated virus-specific RNA isolated from plasma of chicken infected by avian myeloblastosis virus. Unprocessed and processed myeloblastosis-associated virus env-polypeptide precursors.
The 60 S viral RNA complex isolated from leukaemic plasma of chicken infected by avian myeloblastosis virus (AMV) was denatured, the poly(A)-RNA selected and centrifuged in a linear sucrose density gradient. RNA from each fraction was translated in vitro and the products were analyzed by slab polyacrylamide gel electrophoresis (PAGE). Unprocessed primary translation product (p64env) of MAV env gene from 21 S RNA fraction was immunoprecipitated by anti-gp85 serum. If, however, this RNA was translated in the presence of dog pancreas microsomal membranes (DPM), the processed 92 K MAV glycoprotein precursor (p92env) was immunoprecipitated by anti-gp85 serum. This precursor, unlike p64env was resistant to exogenous protease.
Malý
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Institute of Molecular Genetics, Czech Academy of Sciences, Prague.
D000818:Animals; D001353:Avian Leukosis / Q000382:microbiology; D009189:Avian Myeloblastosis Virus / Q000235:genetics*; D002474:Cell-Free System; D002645:Chickens; D015686:Gene Products, env / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D015752:Genes, env; D006031:Glycosylation; D011061:Poly A / Q000235:genetics; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012313:RNA / Q000235:genetics; D012322:RNA Precursors / Q000097:blood / Q000235:genetics*; D012333:RNA, Messenger; D012367:RNA, Viral / Q000097:blood / Q000235:genetics; D012192:Retroviridae Infections / Q000382:microbiology; D012191:Retroviridae Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism
D015686:Gene Products, env; D011498:Protein Precursors; D012322:RNA Precursors; D012333:RNA, Messenger; D012367:RNA, Viral; D012191:Retroviridae Proteins; D011061:Poly A; D012313:RNA