title: Identification of hyaluronan binding proteins using a biotinylated hyaluronan probe.
abstract: A method for detecting hyaluronan (HA)-binding proteins in transblot assays using biotinylated HA (BHA) is described. Some of the binding characteristics of a novel HA receptor termed RHAMM (Receptor for HA-Mediated Motility) are characterized using this assay. The method is also used to detect other HA-binding proteins in tissue homogenates. This method is semiquantitative, rapid, reproducible, sensitive and therefore of potential use in identifying the levels of HA-binding proteins in different cells and tissues.
authors.affiliation: Department of Pediatrics and Child Health, Manitoba Institute of Cell Biology, Winnipeg, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Hoare
authors.forename: R C
authors.initials: RC
authors.lastname: Savani
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.forename: B
authors.initials: B
authors.lastname: Yang
authors.forename: E A
authors.initials: EA
authors.lastname: Turley
mesh_terms: D000818:Animals; D001710:Biotin / Q000378:metabolism; D002352:Carrier Proteins / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid / Q000032:analysis; D008722:Methods; D015335:Molecular Probes / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface / Q000032:analysis*; D016201:Receptors, Lymphocyte Homing / Q000032:analysis*
chemical_list: D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D015335:Molecular Probes; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D001710:Biotin; D006820:Hyaluronic Acid