title: [Isolation and characteristics of an ordered library of transcribed sequences of human chromosome 19 from hybrid human-hamster cells].
abstract: A new technique based on Alu-PCR amplification of hn-RNA is described for the extraction of human-specific transcribed sequences from a hybrid cell line. Arrayed library of hn-cDNA was constructed and characterized by sequencing about 80 individual clones. A high enrichment by human-specific sequences (about 95%) was demonstrated.
authors.forename: D
authors.initials: D
authors.lastname: Obradovich
authors.forename: A M
authors.initials: AM
authors.lastname: Borodin
authors.forename: E P
authors.initials: EP
authors.lastname: Kopantsev
authors.forename: L L
authors.initials: LL
authors.lastname: Vagner
authors.forename: S V
authors.initials: SV
authors.lastname: Volik
authors.forename: O D
authors.initials: OD
authors.lastname: Ermolaeva
authors.forename: Iu B
authors.initials: IuB
authors.lastname: Lebedev
authors.forename: G S
authors.initials: GS
authors.lastname: Monastyrskaia
authors.forename: E D
authors.initials: ED
authors.lastname: Sverdlov
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D006224:Cricetinae; D017931:DNA Primers; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D012313:RNA