title: [Protein determination by binding with the dye Coomassie brilliant blue G-250].
abstract: Possible applications of the Coomassie brilliant blue G-250 dye method for protein assay are reviewed. The physico-chemical and optical properties of the dye are considered in detail. The data on the protein-binding mechanism are generalized. Modifications of the Coomassie blue G-250 quantitative protein assays are critically considered. The applications of the method in medicine, biochemistry and biotechnology are discussed.
authors.forename: V S
authors.initials: VS
authors.lastname: Gasparov
authors.forename: V G
authors.initials: VG
authors.lastname: Degtiar'
mesh_terms: D000818:Animals; D001665:Binding Sites; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D011506:Proteins / Q000378:metabolism*; D012015:Reference Standards; D012394:Rosaniline Dyes / Q000737:chemistry / Q000378:metabolism*; D013057:Spectrum Analysis; D013194:Staining and Labeling
chemical_list: D011506:Proteins; D012394:Rosaniline Dyes; C004692:coomassie Brilliant Blue