Function of bone marrow stromal cell lines derived from nude mice.
We previously reported that in double deficient nude.xid mice B cells failed to develop and their bone marrow did not produce mature B cells in vitro. However, when progenitors from nude.xid bone marrow were placed on a preestablished normal stromal cell line (AC6) they differentiated into surface IgM+ cells. This raised the possibility of a deficiency of nude and nude.xid stromal cells such that they were incapable of supporting the maturation of X-linked immune deficiency (xid) B cells. Here we ask whether bone marrow stromal cells from nude and nude.xid mice have the ability to support xid B cell lymphopoiesis. A primary stromal cell layer derived from nude mice supported xid B cell differentiation in vitro. We derived panels of stromal cell lines by transfection of primary stromal cell layers with a retrovirus encoding SV40 large T Ag. Several bone marrow stromal cell lines derived from nude and nude.xid mice supported xid B cell differentiation from CD43+/CD45 (B220-) to CD45 (B220+) and from CD45 (B220+)/surface IgM- to surface IgM+. Supporting cell lines expressed both IL-7 and insulin-like growth factor I. The frequencies of bone marrow stromal cells capable of supporting xid B cell differentiation were similar in normal, xid, nude, and nude.xid mice. These results demonstrate that nude and nude.xid mice have bone marrow stromal cells with normal abilities to support B cell maturation.
Dong
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Department of Pathology, Tufts University School of Medicine, Boston, MA.
Wortis
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D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000502:physiology*; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D015870:Gene Expression; D006410:Hematopoiesis; D007153:Immunologic Deficiency Syndromes / Q000503:physiopathology; D066298:In Vitro Techniques; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D015851:Interleukin-7 / Q000235:genetics; D017493:Leukocyte Common Antigens / Q000032:analysis; D051379:Mice; D008808:Mice, Inbred CBA; D008817:Mice, Mutant Strains; D008819:Mice, Nude / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics
D000954:Antigens, Surface; D015851:Interleukin-7; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D017493:Leukocyte Common Antigens