Genetic safety evaluation of pesticides in different short-term tests.
Cyanazine, cyhexatin, dicamba and DNOC are pesticides commonly and broadly used in agriculture pest control. However, there is little information on their toxicity and mutagenicity in human cells and in whole animals. Therefore, UDS assay and SCE assay in human peripheral lymphocytes, and chromosome aberration analysis in bone marrow of rats have been used to assess the DNA-damaging activity of the above pesticides. Cyanazine proved non-genotoxic in all the test systems. Cyhexatin showed only weakly positive results for SCE induction in human lymphocytes, providing no concern for genotoxicological hazard. While dicamba did not show clastogenic effects in rodents, DNOC gave significant dose-related increases of structural chromosome aberrations in rat bone marrow cells. Female animals showed increased sensitivity to the toxic effects by DNOC at the highest dose. The results provide further information on the intrinsic genotoxic activity of the tested pesticides, which may contribute to the toxicological assessment of the risk associated with human exposure.
Hrelia
P
P
Department of Pharmacology, University of Bologna, Italy.
Vigagni
F
F
Maffei
F
F
Morotti
M
M
Colacci
A
A
Perocco
P
P
Grilli
S
S
Cantelli-Forti
G
G
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D002869:Chromosome Aberrations; D003996:Dicamba / Q000633:toxicity; D004138:Dinitrocresols / Q000633:toxicity; D005069:Evaluation Studies as Topic; D005260:Female; D006540:Herbicides / Q000633:toxicity; D006801:Humans; D007306:Insecticides / Q000633:toxicity; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*; D010575:Pesticides / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D014220:Trialkyltin Compounds / Q000633:toxicity; D014227:Triazines / Q000633:toxicity
D004138:Dinitrocresols; D006540:Herbicides; D007306:Insecticides; D009153:Mutagens; D010575:Pesticides; D014220:Trialkyltin Compounds; D014227:Triazines; C024132:4,6-dinitro-o-cresol; C001765:cyhexatin; D003996:Dicamba; C007168:cyanazine