Pretreatment with interleukin-1 enhances survival of sympathetic ganglionic neuron grafts.
The effects of pretreatment with interleukin-1 (IL-1) on neurite growth and cell survival of rat superior cervical ganglion (SCG) explants were examined. Neonate rat SCG explants were cultured with serum-containing growth media. Pretreatment with IL-1 beta (30 U/ml) for 3 hours stimulated neurite outgrowth of the SCG explant, which was inhibited by the addition of anti-nerve growth factor (NGF) antibody to the growth medium. Adult rat autologous SCG with the same pretreatment and non-treated ganglia were transplanted into the lateral ventricle. Histological evaluation of the grafts 2 weeks after transplantation revealed that the pretreated SCG cells survived better. Pretreatment with IL-1 may achieve the NGF-mediated neurotrophic effect in sympathetic ganglionic neurons resulting in enhanced graft survival.
Nakao
N
N
Department of Neurological Surgery, Wakayama Medical College.
Itakura
T
T
Uematsu
Y
Y
Nakai
M
M
Komai
N
N
Furukawa
S
S
D000818:Animals; D000831:Animals, Newborn; D016380:Brain Tissue Transplantation; D046508:Culture Techniques; D005728:Ganglia, Sympathetic / Q000187:drug effects*; D007375:Interleukin-1 / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D009414:Nerve Growth Factors / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D010300:Parkinson Disease / Q000188:drug therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014022:Tissue Survival; D014182:Transplantation, Autologous
D007375:Interleukin-1; D009414:Nerve Growth Factors