title: Transcription of the MRP RNA gene in frog stage I oocytes requires a novel cis-element.
abstract: The RNA component of the mitochondrial RNA processing (MRP) enzyme is related to both replication of mitochondrial DNA and processing of 5.8S rRNA, which are accelerated in the frog earliest stage (stage I) of frog oocytes. Microinjection of the deleted genes into the stage I oocytes showed positive cis-elements in the upstream region of the gene. The specific binding of protein(s) to this region was detected in cell extracts from stage I oocytes and liver but not in extracts of stage II-IV oocytes and the concentration of this protein was 40 times higher in the extract of stage I oocytes than that in liver.
authors.affiliation: Department of Biochemistry, Jichi Medical School, Tochigi-ken, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Koike
authors.forename: N
authors.initials: N
authors.lastname: Inohara
authors.forename: I
authors.initials: I
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Tamada
authors.forename: Y
authors.initials: Y
authors.lastname: Kagawa
authors.forename: S
authors.initials: S
authors.lastname: Ohta
mesh_terms: D000818:Animals; D001483:Base Sequence; D002846:Chromatography, Affinity; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D017931:DNA Primers; D004261:DNA Replication; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism; D004722:Endoribonucleases / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D017353:Gene Deletion; D015971:Gene Expression Regulation, Enzymologic; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D009865:Oocytes / Q000201:enzymology / Q000502:physiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D012313:RNA / Q000096:biosynthesis; D000077278:RNA, Mitochondrial; D012340:RNA, Ribosomal, 5.8S / Q000096:biosynthesis; D011898:Ranidae; D015183:Restriction Mapping; D012685:Sepharose / Q000031:analogs & derivatives; D014158:Transcription, Genetic; D014982:Xenopus laevis
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D000077278:RNA, Mitochondrial; D012340:RNA, Ribosomal, 5.8S; C037405:heparin-sepharose; D012313:RNA; D004247:DNA; D012685:Sepharose; D004722:Endoribonucleases; C058330:mitochondrial RNA-processing endoribonuclease