Lindane affects phosphoinositide turnover through a different mechanism of the phosphatidylinositol synthesis inhibition in rat renal proximal tubule cell culture.
The ability of lindane to change the metabolism of inositol phospholipids was investigated using rat renal proximal tubular cell cultures labelled with [3H]inositol. Lindane addition to the culture medium caused labelling of inositol trisphosphate, inositol bisphosphate and inositol tetrakisphosphate to decrease and that of the inositol monophosphate pool to increase. A depletion of radioactivity in phosphatidylinositols was also observed after lindane addition. Most strikingly, the addition of lindane considerably increased the levels of glycerophosphoinositol in a dose-dependent manner. The effect of lindane follows a different pattern from that of bradykinin, and it is suggested to act by stimulating phospholipase A activity(ies).
Senar
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Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, Madrid, Spain.
Recio
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Pérez-Albarsanz
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D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D001556:Hexachlorocyclohexane / Q000494:pharmacology*; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010716:Phosphatidylinositols / Q000096:biosynthesis* / Q000378:metabolism*; D051381:Rats
D003470:Culture Media; D010716:Phosphatidylinositols; D001556:Hexachlorocyclohexane