Evaluation of magnetic alginate beads as a solid support for positive selection of CD34+ cells.
Paramagnetic alginate beads with a 10-100 microns size range have been developed. These beads, when activated with chloroacetic anhydride and covalently coupled to avidin (30 micrograms/mg beads), were able to bind biotinylated goat anti-mouse (B-GAM) antibody (Ab). The beads with immobilized antibody were then used as a model system for the capture and non-enzymatic release of CD34+ (KG1a) cells. A maximum of 82% KG1a (average = 65 +/- 16.1%) cell capture, and 57% (average = 51 +/- 5.9%) cell release has been attained using this model system. Optimization of the system in terms of further bead size reduction, and in terms of developing a system to recover released cells with high purity, will make an excellent system for cell capture and nonenzymatic release.
Pope
N M
NM
Baxter Healthcare Corporation, Biotech Group-Immunotherapy Division, Santa Ana, California 92705.
Alsop
R C
RC
Chang
Y A
YA
Smith
A K
AK
D000464:Alginates / Q000737:chemistry*; D000818:Animals; D000937:Antigen-Antibody Reactions; D015703:Antigens, CD / Q000737:chemistry*; D018952:Antigens, CD34; D001360:Avidin; D001710:Biotin; D002460:Cell Line; D005069:Evaluation Studies as Topic; D006041:Goats / Q000276:immunology; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D008280:Magnetics; D051379:Mice / Q000276:immunology; D008863:Microspheres; D010316:Particle Size
D000464:Alginates; D015703:Antigens, CD; D018952:Antigens, CD34; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D001360:Avidin; D001710:Biotin