Nonselective cation channels in cells of the crypt-base of rat distal colon.
Cells in the base of isolated intact crypts of rat distal colon were investigated with the slow whole-cell patch-clamp technique with nystatin in the patch pipette. Addition of either prostaglandin E2 or forskolin to the bath depolarized the cell from -74 mV to -27 mV. This depolarization was reversed when bath Na+ was replaced by N-methyl-D-glucamine (NMDG+), or when flufenamic acid (50 microM) was added to the bath. In cell-attached and cell-excised patches of the basolateral membrane nonselective cation channels (gamma = 38 pS, 35 degrees C) were recorded. It is concluded that nonselective cation channels are activated by PGE2 and forskolin. The channels could be involved in cell proliferation.
Siemer
C
C
Max-Planck-Institut für Biophysik, Frankfurt, FRG.
Gögelein
H
H
D000818:Animals; D002412:Cations / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D005576:Colforsin / Q000494:pharmacology; D003106:Colon / Q000166:cytology / Q000502:physiology*; D015232:Dinoprostone / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D005439:Flufenamic Acid / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D008536:Meglumine / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D009130:Muscle, Smooth / Q000166:cytology / Q000502:physiology*; D051381:Rats; D012964:Sodium / Q000494:pharmacology
D002412:Cations; D007473:Ion Channels; D005576:Colforsin; D005439:Flufenamic Acid; D008536:Meglumine; D012964:Sodium; D015232:Dinoprostone