Inhibition of the bacterial mutagenicity of N-methyl-N'-nitro-N-nitrosoguanidine by ascorbic acid and ascorbyl palmitate.
The mechanism of antimutagenic activity of ascorbic acid (AA) and its derivatives was studied using the Salmonella typhimurium TA100 bacterial test system. All substances studied inhibited N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced mutagenesis. Ascorbyl palmitate (AP) markedly decreased the numbers of his+ revertants, behaving as a membrane-active antimutagen. A comparative study of the antioxidative activity of the investigated substances in the methyl oleate (MO) system has demonstrated that AA and its derivatives have pro-oxidant properties within the limits of the concentrations studied. The results obtained do not agree with the common view of the mode of action of these antimutagens, including both inhibition of free radical processes and MNNG reductive inactivation.
Tyrsina
E G
EG
Branch of Kurchatov Atomic Energy Institute, Moscow Region, Russia.
Rossikhina
O G
OG
Abilev
S K
SK
Tyrsin YuA
D016587:Antimutagenic Agents / Q000378:metabolism / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D001711:Biotransformation; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals; D008563:Membrane Lipids / Q000378:metabolism; D008769:Methylnitronitrosoguanidine / Q000378:metabolism / Q000633:toxicity*; D009152:Mutagenicity Tests; D009829:Oleic Acids / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism; D010084:Oxidation-Reduction; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013489:Suppression, Genetic
D016587:Antimutagenic Agents; D000975:Antioxidants; D005609:Free Radicals; D008563:Membrane Lipids; D009829:Oleic Acids; D016877:Oxidants; D008769:Methylnitronitrosoguanidine; C005576:methyl oleate; D001205:Ascorbic Acid; C031226:6-O-palmitoylascorbic acid