Generation and characterization of a human monoclonal IgG antibody specific for HLA-B12.
We describe here the generation and characterization of a human monoclonal IgG antibody (UL/F14) specific for HLA-B12. The antibody is suitable for complement-dependent lysis on lymphoblastoid cell lines and stimulated peripheral blood mononuclear cells. UL/F14 competes for antigen binding with an HLA-B12 human monoclonal IgM antibody and with a specific alloantiserum. Protein chemistry shows that the monoclonal antibody UL/F14 can precipitate solubilized HLA-B12 molecules.
Siemoneit
K
K
Department of Transplantation Immunology, Red Cross Blood Bank, Ulm, FRG.
Wölpl
A
A
Wegener
S
S
Ernst
M
M
Sonneborn
H H
HH
Goldmann
S F
SF
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D001667:Binding, Competitive; D002461:Cell Line, Transformed; D003165:Complement System Proteins / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D015235:HLA-B Antigens / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G / Q000276:immunology* / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000276:immunology; D008213:Lymphocyte Activation; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015235:HLA-B Antigens; C035015:HLA-B12 antigen; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003165:Complement System Proteins