The E. coli recA gene can restore the defect in mutagenesis of the pso4-1 mutant of S. cerevisiae.
The E. coli recA gene was introduced into the pso4-1 mutant of S. cerevisiae and transformants were treated with 8-MOP+UVA and 254-nm UV light. The results showed that the recA gene increased the resistance to the toxic effect of 8-MOP+UVA and restored the frequency of reversion of the pso4-1 mutants after both treatments. The presence of the recA gene stimulated expression of the small subunit of the ribonucleotide reductase (Rnr2) in the pso4-1 mutants. Thus the E. coli recA gene is functional in yeast. Moreover, it was shown that the pso4-1 mutant is epistatic to pso1-1 and rad6-1, which belong to a mutagenic repair pathway. We propose here that the PSO4 gene has some role in the control of mutagenic repair in yeast.
Morais Júnior
M A
MA
Departamento de Biofísica e Centro de Biotecnologia, UFRGS, Porto Alegre, RS, Brazil.
Brozmanová
J
J
Benfato
M S
MS
Duraj
J
J
Vlcková
V
V
Henriques
J A
JA
D004260:DNA Repair; D004843:Epistasis, Genetic; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics; D008730:Methoxsalen / Q000494:pharmacology; D016296:Mutagenesis; D009154:Mutation; D011938:Rec A Recombinases / Q000032:analysis / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D014170:Transformation, Genetic; D014466:Ultraviolet Rays
D011938:Rec A Recombinases; D008730:Methoxsalen