title: A previously unidentified choline acetyltransferase transcript in the human foetal brain.
abstract: To identify choline acetyltransferase (ChAT) transcript(s) in human foetal brain we used the reverse polymerase chain reaction including different couples of oligonucleotide primers. The analysis indicated the presence of a new choline acetyltransferase transcript containing at its 5' end an untranslated exon (E1A) of 57 nucleotides in length extending from Nt 3771 to Nt 3828. It differs in the 5'-non coding region from the M-N-R type mRNA previously described in mouse and rat.
authors.affiliation: Laboratoire de génétique moléculaire des eucaryotes, CNRS, U184 INSERM, Faculté de Médecine, Strasbourg, France.
authors.forename: M
authors.initials: M
authors.lastname: Schmitt
authors.forename: J M
authors.initials: JM
authors.lastname: Garnier
authors.forename: P
authors.initials: P
authors.lastname: Simoni
authors.forename: C
authors.initials: C
authors.lastname: Quirin-Stricker
mesh_terms: D001921:Brain / Q000196:embryology* / Q000201:enzymology; D002795:Choline O-Acetyltransferase / Q000235:genetics*; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase; D014158:Transcription, Genetic
chemical_list: D015345:Oligonucleotide Probes; D002795:Choline O-Acetyltransferase; D012194:RNA-Directed DNA Polymerase