Predominant induction of kinetochore-containing micronuclei by extracts of diesel exhaust particulates in cultured human lymphocytes.
The aneuploidy-inducing activity of extracts of diesel exhaust particulates from light duty (LD) and heavy duty (HD) engines was investigated in cultured peripheral blood lymphocytes of 8 healthy donors using the cytokinesis-block micronucleus test with the kinetochore labelling modification. A majority of the subjects tested showed a significant kinetochore-positive micronucleus induction after treatment with the highest dose (150 micrograms/ml) of LD extract, although some subjects also showed induction of kinetochore-negative micronuclei. Only one subject had significantly increased numbers of kinetochore-positive micronuclei at a dose of 400 micrograms/ml of HD extract. These results suggest that diesel extract, at least LD extract, possesses the ability to induce whole chromosome loss (aneuploidy) preferentially, although there are also chromosome breaks.
Odagiri
Y
Y
Department of Public Health, Saitama Medical School, Japan.
Zhang
J X
JX
Uchida
H
H
Kawamura
K
K
Adachi
S
S
Takemoto
K
K
D000328:Adult; D000974:Antibodies, Antinuclear; D002455:Cell Division / Q000187:drug effects; D002503:Centromere / Q000187:drug effects*; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D015162:Micronucleus Tests / Q000379:methods; D016296:Mutagenesis; D009153:Mutagens / Q000633:toxicity*; D008941:Spindle Apparatus / Q000187:drug effects; D001335:Vehicle Emissions / Q000633:toxicity*
D000974:Antibodies, Antinuclear; D009153:Mutagens; D001335:Vehicle Emissions