Identification of a novel neutrophil membrane protein involved in modulation of oxidative burst.
On the basis of selective recognition by antibodies directed against neutrophil membrane determinants, a new neutrophil protein (molecular mass 82 kDa) has been identified, and shown to be functionally correlated with the oxidative response evoked in these cells by agonist stimulation. The protein is present in neutrophil membrane fraction but only upon activation it becomes accessible to recognition by a specific monoclonal antibody. In these conditions a complete and selective inhibition of O2- production occurs. The presence of a new protein antigen in neutrophil membranes linked to the activation of the O2- producing multienzyme complex that becomes external to the cell surface in primed or activated cells, might be important for future approaches aiming at the control of neutrophil response and at the identification of the activated forms of these cells.
Michetti
M
M
Institute of Biochemistry, University of Genoa, Italy.
De Tullio
R
R
Melloni
E
E
Pontremoli
S
S
D000911:Antibodies, Monoclonal; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D009504:Neutrophils / Q000378:metabolism*; D016897:Respiratory Burst; D013481:Superoxides / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000939:Epitopes; D008565:Membrane Proteins; D013481:Superoxides