[Separation of hemoglobins F, Fac, S, C, A1c and determination of hemoglobin F using high performance liquid chromatography].
Separation of hemoglobins F, Fac, S, C and A1c was performed using high performance liquid chromatography with a cation exchange Polycat A column in a 15-minute assay. HbF titration results were well correlated with those of the reference alkali denaturation technique for values below 12% (r = 0.95; P < 0.001). This technique may be used as a confirmation test for neonatal screening of sickle cell disease.
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Laboratoire d'hématologie, hôpital Necker, Paris, France.
Ducrocq
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Girot
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D000755:Anemia, Sickle Cell / Q000517:prevention & control; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005319:Fetal Hemoglobin / Q000032:analysis* / Q000302:isolation & purification; D006442:Glycated Hemoglobin / Q000302:isolation & purification; D006444:Hemoglobin C / Q000302:isolation & purification; D006451:Hemoglobin, Sickle / Q000302:isolation & purification; D006454:Hemoglobins / Q000302:isolation & purification*; D006801:Humans; D007231:Infant, Newborn; D008403:Mass Screening
D005319:Fetal Hemoglobin; D006442:Glycated Hemoglobin; D006444:Hemoglobin C; D006451:Hemoglobin, Sickle; D006454:Hemoglobins