title: Expression of adhesion molecules in poststreptococcal glomerulonephritis.
abstract: Because adhesion properties of leukocytes are important for the influx and localization of leukocytes in sites of inflammation, we studied, the expression of intercellular adhesion molecule 1 (ICAM-1), lymphocyte-function-associated antigen 1 (LFA-1), vascular cell adhesion molecule- 1 (VCAM-1) and CD 11b in 14 kidney biopsies of PSGN patients, arbitrarily divided into early biopsies (less than 15 days after onset of PSGN) and late biopsies (17-90 days). In PSGN, intraglomerular ICAM-1 expression was increased in early biopsies (score 3.1 +/- SEM 0.2; P < 0.005) and decreased with time; in late biopsies the score (2.0 +/- 0.2) was similar to that of normal kidney (1.3 +/- 0.3). In the interstitium ICAM-1 was increased (early PSGN = 836 +/- 56 positive cells/mm2, late = 552 +/- 60.0; versus normal = 364 +/- 12.4; P < 0.05). LFA-1 expressing cells in glomeruli were also increased in early biopsies (10.0 +/- 2.1 positive cells per glomerular cross-section (gcs), versus normal 2.9 +/- 1.4; P < 0.05). In the interstitium, LFA-1 positive cells were increased (early PSGN = 221 +/- 79.6 cells/mm2, late PSGN = 134.5 +/- 45.1, normal = 21 +/- 8.7; P < 0.05). VCAM-1 in glomeruli and interstitium was not increased in PSGN. Our studies demonstrate increased expression of adhesion molecules ICAM-1 and LFA-1 in the kidney of PSGN patients, and these findings were more pronounced in early biopsies; adhesion molecules are probably involved in the inflammatory infiltration of this disease.
authors.affiliation: Renal Service and Laboratory, Hospital Universitario de Maracaibo, Venezuela.
authors.forename: G
authors.initials: G
authors.lastname: Parra
authors.forename: M
authors.initials: M
authors.lastname: Romero
authors.forename: C
authors.initials: C
authors.lastname: Henriquez-La Roche
authors.forename: R
authors.initials: R
authors.lastname: Pineda
authors.forename: B
authors.initials: B
authors.lastname: Rodríguez-Iturbe
mesh_terms: D000293:Adolescent; D000911:Antibodies, Monoclonal; D018845:CD11 Antigens / Q000032:analysis; D015815:Cell Adhesion Molecules / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D015870:Gene Expression; D005921:Glomerulonephritis / Q000276:immunology* / Q000382:microbiology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D007668:Kidney / Q000276:immunology* / Q000382:microbiology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D008297:Male; D009000:Monocytes / Q000276:immunology; D013290:Streptococcal Infections / Q000150:complications*; D013297:Streptococcus pyogenes; D013601:T-Lymphocytes / Q000276:immunology; D019010:Vascular Cell Adhesion Molecule-1
chemical_list: D000911:Antibodies, Monoclonal; D018845:CD11 Antigens; D015815:Cell Adhesion Molecules; D016169:Lymphocyte Function-Associated Antigen-1; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1