Evaluation of a rodent peroxisome proliferator in two species of freshwater fish: rainbow trout (Onchorynchus mykiss) and Japanese medaka (Oryzias latipes)
Rainbow trout (Onchorynchus mykiss) and Japanese medaka (Oryzias latipes) were exposed to the hypolipidemic drug gemfibrozil, a known rodent peroxisome proliferator. Trout were injected (i.p.) daily for 2 weeks at doses of 0, 46, 87, or 152 mg/kg/day. Medaka were exposed to the nominal concentrations of 0, 1.25, 2.5, or 5 ppm in water for 2 weeks in a static-renewal system. Peroxisome proliferation was assessed by measuring fatty acyl-CoA oxidase (FAO) activity, peroxisomal bifunctional enzyme (PBE) quantity, and changes in liver-to-body weight ratios (LWR). Results indicate that a mild peroxisome proliferative response was observed in rainbow trout (significant increases in FAO activity at all dose levels and in LWR at the highest dose level). Medaka demonstrated a significant increase in PBE at the highest dose level, while nonsignificant increases in FAO activity were observed at the mid- and high-dose levels.
Scarano
L J
LJ
Environ Corporation, Arlington, Virginia 22203.
Calabrese
E J
EJ
Kostecki
P T
PT
Baldwin
L A
LA
Leonard
D A
DA
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D005260:Female; D015248:Gemfibrozil / Q000494:pharmacology; D007274:Injections, Intraperitoneal; D008099:Liver / Q000187:drug effects; D008297:Male; D008830:Microbodies / Q000187:drug effects* / Q000502:physiology; D017686:Oncorhynchus mykiss; D009929:Organ Size / Q000187:drug effects; D009990:Oryzias; D012377:Rodentia; D014874:Water Pollutants, Chemical / Q000494:pharmacology
D014874:Water Pollutants, Chemical; D015248:Gemfibrozil