Interferon-gamma regulation of interleukin 6 in monocytic cells.
The regulation of interleukin 6 (IL-6) is dependent on many factors that include numerous stimuli such as lipopolysaccharide (LPS), viruses, and other cytokines. These studies demonstrate the ability of interferon-gamma (IFN-gamma) to significantly enhance IL-6 mRNA and protein production in LPS-stimulated monocytes and THP-1 cells. IL-6 protein production was increased sevenfold in LPS-stimulated cells with the addition of IFN-gamma. IL-6 mRNA production was increased in a dose-dependent fashion up to 15-fold in LPS-stimulated cells with the addition of IFN-gamma. The enhanced production of IL-6 mRNA that occurs with the addition of IFN-gamma to LPS-stimulated monocytic cells was due to increased transcription of IL-6 mRNA. The ability of IFN-gamma to enhance IL-6 production may play an important role in many inflammatory processes.
Biondillo
D E
DE
Department of Medicine, Indiana University School of Medicine, Indianapolis 46202.
Konicek
S A
SA
Iwamoto
G K
GK
D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D006207:Half-Life; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides / Q000494:pharmacology; D009000:Monocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015850:Interleukin-6; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007371:Interferon-gamma