Human testis cDNAs identified by sera from infertile patients: a molecular biological approach to immunocontraceptive development.
Sera from patients with known or suspected immunological infertility were used to screen a human testis cDNA library. A total of 59 sera detected 38 unique cDNA inserts of which four were testis specific by Northern blot analyses. One of these is a testis-specific isoform of calpastatin. Five additional clones, although not testis specific, were found to be testis abundant. The number and type of clones identified by these human sera suggests a possible aetiology for immunologic infertility. The testis-specific clones will be further characterized to establish their usefulness as contraceptive vaccine candidates.
Liang
Z G
ZG
Center for Recombinant Gamete Contraceptive Vaccinogens, Northwestern University, Evanston, IL 60208.
O'Hern
P A
PA
Yavetz
B
B
Yavetz
H
H
Goldberg
E
E
D000941:Antigens / Q000235:genetics / Q000302:isolation & purification*; D002999:Clone Cells; D003269:Contraception, Immunologic; D004262:DNA Restriction Enzymes; D004251:DNA Transposable Elements / Q000235:genetics; D018076:DNA, Complementary / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006801:Humans; D007248:Infertility, Male / Q000097:blood / Q000276:immunology*; D008297:Male; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000276:immunology*
D000941:Antigens; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D000939:Epitopes; D004262:DNA Restriction Enzymes