Growth hormone receptors in avian epiphyseal growth-plate chondrocytes.
Growth hormone receptor (GH-R) gene expression was evaluated in avian growth-plate cartilage by Northern blot and hybridization using the avian GH-R probe. A single transcript of approximately 5.2 kb was demonstrated in cultured growth-plate chondrocytes as well as in growth-plate extracts. GH receptor gene expression was inhibited by chicken GH (cGH) in a dose- and time-dependent manner. Chicken GH was more potent in down-regulating the GH-R gene expression than hGH, but on the other hand cGH exhibited a lower affinity to avian chondrocytes receptor than did the human hormone. Addition of ascorbic acid to the culture media caused cell differentiation: induction of alkaline phosphatase activity and attenuation of collagen type II gene expression. No differences in the GH-R gene expression were observed in the nondifferentiated cells compared with the differentiated cells. Chicken GH did not form any complex with the purified hGH binding protein (hGHBP), did not bind to human lymphocytes GH receptor, and did not affect Nb2 cell proliferation. These systems represent somatogenic and lactogenic types of GH receptors, respectively. In summary, avian growth-plate chondrocytes in situ and in culture exhibit GH-R and these receptors are capable of binding GH. Thus, the failure of GH to affect avian chondrocytes' proliferation was not due to either the absence of receptors on the cell membrane or to a lack in its binding activity, but rather may be due to events farther downstream.
Monsonego
E
E
Institute of Animal Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Halevy
O
O
Gertler
A
A
Volokita
M
M
Schickler
M
M
Hurwitz
S
S
Pines
M
M
D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001717:Birds / Q000378:metabolism*; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D002645:Chickens; D015870:Gene Expression / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology; D006132:Growth Plate / Q000166:cytology* / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis; D011986:Receptors, Somatotropin / Q000096:biosynthesis*
D002352:Carrier Proteins; D011986:Receptors, Somatotropin; D012313:RNA; D013006:Growth Hormone; D000469:Alkaline Phosphatase; C051815:somatotropin-binding protein