Production of monoclonal antibodies against epitopes of the main coat protein of filamentous fd phages.
Three monoclonal antibodies (MAbs) were produced which react with epitopes of the main structural coat protein (pVIII) of filamentous fd phages as demonstrated by solid-phase fluorometric enzyme immunoassays and by immunoelectron microscopy. The antibodies are of the IgG1, IgG2a and IgG2b immunoglobulin subclasses. Since they also react with recombinant phages expressing antigen fragments in their pIII region they may be suitable reagents for the demonstration and isolation of filamentous phages used in recombinant protein technology.
Micheel
B
B
Antibody Modification Group, Max Delbrück Centre for Molecular Medicine, Berlin-Buch, Germany.
Heymann
S
S
Scharte
G
G
Böttger
V
V
Vogel
F
F
Dübel
S
S
Breitling
F
F
Little
M
M
Behrsing
O
O
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000378:metabolism; D001666:Binding Sites, Antibody; D002213:Capsid / Q000276:immunology*; D002999:Clone Cells; D000939:Epitopes / Q000276:immunology*; D006822:Hybrid Cells; D017912:Inovirus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes