Immunostaining for Leu-7 in the diagnosis of thyroid carcinoma.
The morphologic distinction between benign and malignant thyroid lesions can be difficult. Immunostaining for the Leu-7 antigen has been reported to be useful in the diagnosis of thyroid carcinoma. To test the specificity of immunostaining for Leu-7 in the diagnosis of thyroid carcinoma, we studied formalin-fixed, paraffin-embedded tissue specimens obtained from 66 thyroid lesions using the HNK-1 monoclonal antibody to Leu-7 and an avidin-biotin-complex technique. Positive reactivity for Leu-7 was seen in 11 (33%) of 33 benign thyroid conditions and in 27 (82%) of 33 thyroid carcinomas. We conclude that immunostaining for Leu-7 is not specific in the diagnosis of thyroid carcinoma.
Loy
T S
TS
Department of Pathology, University of Missouri Medical School, Columbia.
Darkow
G V
GV
Spollen
L E
LE
Diaz-Arias
A A
AA
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D001360:Avidin; D001710:Biotin; D018959:CD57 Antigens; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007158:Immunologic Techniques; D013194:Staining and Labeling; D013964:Thyroid Neoplasms / Q000378:metabolism / Q000473:pathology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018959:CD57 Antigens; D001360:Avidin; D001710:Biotin