title: Effect of a derivatized dextran on human osteoblast growth and phenotype expression.
abstract: Water soluble derivatized dextran named E9 with a molecular weight of 45,000 g l-1 containing 58% methyl carboxylic acid unit, 19% benzylamide unit, and 26% sulfonate with a specific anticoagulant activity of 0.29 IU mg-1 was studied for its effects on human osteoblast growth and phenotype expression for short-term treatment. At concentrations between 1 ng ml-1 and 1 microgram ml-1 E9 has no effect on DNA synthesis whereas at higher concentrations DNA synthesis is inhibited in a dose related fashion (87% for 400 micrograms ml-1). For concentrations which do not modify osteoblast growth, E9 promotes alkaline phosphatase activity, type I collagen and osteocalcin synthesis with a maximum effect for 0.1-1 microgram ml-1. It has a synergistic effect with hPTH increasing AMPc. Moreover, osteonectin synthesis was enhanced in a dose-dependent manner between 0.1 and 5 micrograms ml-1. These results seem to indicate that E9 is able to stimulate human osteoblast phenotype expression and could be useful in clinical applications.
authors.affiliation: INSERM-U, 306, Université de Bordeaux II, France.
authors.forename: S
authors.initials: S
authors.lastname: Berrada
authors.forename: J
authors.initials: J
authors.lastname: Amedee
authors.forename: T
authors.initials: T
authors.lastname: Avramoglou
authors.forename: J
authors.initials: J
authors.lastname: Jozefonvicz
authors.forename: M F
authors.initials: MF
authors.lastname: Harmand
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D000242:Cyclic AMP / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis*; D003911:Dextrans / Q000494:pharmacology*; D006801:Humans; D008875:Middle Aged; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015675:Osteocalcin / Q000032:analysis / Q000378:metabolism; D015676:Osteonectin / Q000032:analysis / Q000378:metabolism; D010641:Phenotype; D011392:Proline / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
chemical_list: D001672:Biocompatible Materials; D003911:Dextrans; D015676:Osteonectin; D014316:Tritium; D015675:Osteocalcin; D003094:Collagen; D004247:DNA; D011392:Proline; D000242:Cyclic AMP; D000469:Alkaline Phosphatase; D013936:Thymidine