title: Aeromonas salmonicida resistance to complement-mediated killing.
abstract: The resistance of Aeromonas salmonicida to complement-mediated killing was investigated by using different strains and their isogenic mutants that had been previously characterized for their surface components. We found that the classical complement pathway is involved in serum killing of susceptible A. salmonicida strains, while the alternative complement pathway seems not to be involved. All of the A. salmonicida strains are able to activate complement, but the smooth strains (with or without the A-layer) are resistant to complement-mediated killing. The reasons for this resistance are that C3b may be bound far from the cell membrane and that it is rapidly degraded; therefore, the lytic final complex C5b-9 (membrane attack complex) is not formed. Isogenic rough mutants are serum sensitive because they bind more C3b than the smooth strains, and if C3b is not completely degraded, then the lytic complex (C5b-9) is formed.
authors.affiliation: Departamento de Microbiología, Universidad de Barcelona, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Merino
authors.forename: S
authors.initials: S
authors.lastname: Albertí
authors.forename: J M
authors.initials: JM
authors.lastname: Tomás
mesh_terms: D000333:Aeromonas / Q000276:immunology* / Q000648:ultrastructure; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001770:Blood Bactericidal Activity; D003167:Complement Activation; D003179:Complement C3b / Q000378:metabolism; D003182:Complement C5 / Q000378:metabolism; D050776:Complement C5b; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology; D016253:Microscopy, Immunoelectron; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000276:immunology; D011817:Rabbits; D014337:Trout
chemical_list: D000942:Antigens, Bacterial; D003182:Complement C5; D015938:Complement Membrane Attack Complex; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D003179:Complement C3b; D050776:Complement C5b