Structure and induction of a lysozyme gene from the tobacco hornworm, Manduca sexta.
Lysozyme is hypothesized to play a central role in initiating and maintaining the antibacterial defense response of Manduca sexta. We isolated a cDNA clone encoding a M. sexta lysozyme. Results of Northern blot analyses using this cDNA as a probe indicated that the abundance of lysozyme transcripts increased in seven tissues following treatment with peptidoglycan, with the highest level of accumulation occurring in the fat body. An analysis of the kinetics of accumulation of the transcripts in the fat body demonstrated low levels of transcripts in the naive larvae which increased rapidly after treatment and remained elevated over several days. A genomic fragment containing a lysozyme gene was also isolated and the nucleotide sequence and transcription start site of the gene was determined.
Mulnix
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Department of Entomology, Purdue University, West Lafayette, IN 47907-1158.
Dunn
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PE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic; D007700:Kinetics; D008969:Molecular Sequence Data; D009036:Moths / Q000201:enzymology / Q000235:genetics*; D009113:Muramidase / Q000096:biosynthesis* / Q000235:genetics*; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D010457:Peptidoglycan / Q000494:pharmacology; D012313:RNA / Q000032:analysis / Q000378:metabolism; D015183:Restriction Mapping; D014158:Transcription, Genetic / Q000187:drug effects
D018076:DNA, Complementary; D015345:Oligonucleotide Probes; D010457:Peptidoglycan; D012313:RNA; D009113:Muramidase