Short consensus repeat-3 domain of recombinant decay-accelerating factor is recognized by Escherichia coli recombinant Dr adhesin in a model of a cell-cell interaction.
A bacterial pathogen that is important in both urinary tract and intestinal infections is Escherichia coli which expresses Dr or related adhesins. In this report, we present a model for testing cell-cell interaction, using both molecularly characterized laboratory cells that express recombinant molecules of human decay-accelerating factor (DAF), and recombinant bacterial Dr colonization factors. Dr adhesin ligand was identified as DAF (CD55), a membrane protein that protects autologous tissues from damage due to the complement system. Structure-function studies mapped the adhesin-binding site on the DAF molecule. A single-point substitution in the third short consensus repeat domain, Ser165 to Leu, corresponding to the Dra to Drb allelic polymorphism, caused complete abolition of adhesin binding to DAF.
Nowicki
B
B
Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston 77550.
Hart
A
A
Coyne
K E
KE
Lublin
D M
DM
Nowicki
S
S
D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial / Q000378:metabolism*; D015703:Antigens, CD / Q000737:chemistry / Q000378:metabolism*; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D001665:Binding Sites; D018958:CD55 Antigens; D002448:Cell Adhesion; D006801:Humans; D066298:In Vitro Techniques; D008024:Ligands; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins; D013329:Structure-Activity Relationship
D018830:Adhesins, Escherichia coli; D000942:Antigens, Bacterial; D015703:Antigens, CD; D001425:Bacterial Outer Membrane Proteins; D018958:CD55 Antigens; D008024:Ligands; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins