Enzymatic synthesis of milligram quantities of ribozymes in small volumes.
Milligram quantities of ribozymes (Rzs) can be synthesized in vitro in reaction volumes of 1 mL or less using AmpliScribe T7 RNA polymerase kits to transcribe either linear plasmids or oligodeoxynucleotide templates. Model hammerhead Rzs were synthesized that specifically cleave RNA-encoding chloramphenicol acetyl transferase sequences. Methods are presented for the transcription of Rzs of virtually any length and sequence composition at a fraction of the cost of chemical synthesis.
Hoffman
L M
LM
Epicentre Technologies, Madison, WI.
Johnson
M G
MG
D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D008969:Molecular Sequence Data; D012313:RNA / Q000378:metabolism; D016337:RNA, Catalytic / Q000096:biosynthesis*; D011933:Reagent Kits, Diagnostic; D014158:Transcription, Genetic
D016337:RNA, Catalytic; D011933:Reagent Kits, Diagnostic; D012313:RNA; D015500:Chloramphenicol O-Acetyltransferase