Human monocyte chemotactic proteins-2 and 3 (MCP-2 and MCP-3) attract human eosinophils and desensitize the chemotactic responses towards RANTES.
When synthetic monocyte chemotactic proteins (MCPs) were tested in a Boyden chamber assay system for eosinophil-chemotactic properties using human eosinophils, MCP-3 was found to be a potent and efficient (percentage input migrating cells) attractant with an ED50 near 2-3 nM. MCP-2 was less potent, exhibiting half maximal chemotaxis at 40 nM. Cross desensitization experiments of eosinophil-chemotaxis revealed that both MCP-3 and MCP-2 desensitized autologous responses. In addition, pretreatment of human eosinophils with natural RANTES desensitized chemotaxis towards MCP-3 as well as MCP-2, whereas pretreatment of eosinophils with MCP-3 desensitized, apart from responses to MCP-3, also responses against RANTES and MCP-2. These findings indicate that possibly both MCP-3 and MCP-2 elicit chemotactic responses via the RANTES-receptor on eosinophils.
Noso
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N
Department of Dermatology, University of Kiel, Germany.
Proost
P
P
Van Damme
J
J
Schröder
J M
JM
D018946:Chemokine CCL5; D054410:Chemokine CCL7; D054412:Chemokine CCL8; D002630:Chemotactic Factors / Q000502:physiology*; D002634:Chemotaxis, Leukocyte; D016207:Cytokines; D004804:Eosinophils / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008222:Lymphokines / Q000502:physiology*; D018945:Monocyte Chemoattractant Proteins; D019713:Receptors, CCR5; D019707:Receptors, Chemokine; D011971:Receptors, Immunologic / Q000502:physiology*
C515450:CCL7 protein, human; C515453:CCL8 protein, human; D018946:Chemokine CCL5; D054410:Chemokine CCL7; D054412:Chemokine CCL8; D002630:Chemotactic Factors; D016207:Cytokines; D008222:Lymphokines; D018945:Monocyte Chemoattractant Proteins; D019713:Receptors, CCR5; D019707:Receptors, Chemokine; D011971:Receptors, Immunologic