title: Hepatitis C virus particle detected by immunoelectron microscopic study.
abstract: To clarify the morphology of hepatitis C virus (HCV), an indirect immunogold electron microscopic study was carried out on two plasma samples with high HCV RNA titres using polyclonal and monoclonal antibodies specific to the putative HCV envelope protein. Spherical virus-like particles, 55 to 65 nm in diameter with spike-like projections, were found in 1.14 to 1.16 g/ml fractions after sucrose density gradient centrifugation. These particles were found only in HCV-infected blood donors and had morphological features similar to those of flaviviruses. Moreover, these particles specifically reacted with the polyclonal and monoclonal antibodies to the putative HCV envelope protein. This is the first known report in which the morphology of the HCV particle is clearly shown.
authors.affiliation: Third Department of Internal Medicine, Mie University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kaito
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Tsukiyama-Kohara
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Konishi
authors.forename: M
authors.initials: M
authors.lastname: Yokoi
authors.forename: S
authors.initials: S
authors.lastname: Ishida
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Kohara
mesh_terms: D000906:Antibodies; D000911:Antibodies, Monoclonal; D016174:Hepacivirus / Q000648:ultrastructure*; D006508:Hepatitis Antibodies / Q000097:blood; D018937:Hepatitis C Antibodies; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D012367:RNA, Viral / Q000097:blood; D014616:Vaccinia virus / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D014771:Virion / Q000648:ultrastructure
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D012367:RNA, Viral; D014759:Viral Envelope Proteins