Effects of exogenous FGFs on growth, differentiation, and survival of chick neural retina cells.
Fibroblast growth factors (FGFs) are known to play important roles in various processes including development and differentiation. Chick embryo neural retina cells, capable of transdifferentiation into lentoid bodies and pigmented cells, were used in vitro to examine the effects of exogenous acidic (aFGF) and basic FGF (bFGF) on proliferation and protein accumulation. We demonstrate that both factors increase the proliferation of glial cells and modulate the survival of neurons without affecting protein accumulation within these cells. Moreover, FGFs stimulate the differentiation of the photoreceptors. The rate of proliferation varies over the period of culture, with a maximum occurring after 2 weeks, followed by a decrease concommitant with the appearance of lentoid bodies. The concentration of aFGF was measured using an enzyme immuno assay and showed an accumulation of this protein only in bFGF-treated cultures, suggesting that bFGF positively modulates aFGF synthesis in neural retina cell cultures.
Tcheng
M
M
Unité de Recherches Gérontologiques, U.118 INSERM, affiliée CNRS, Association Claude Bernard, Paris, France.
Oliver
L
L
Courtois
Y
Y
Jeanny
J C
JC
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002642:Chick Embryo; D003459:Crystallins / Q000302:isolation & purification; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005346:Fibroblast Growth Factors / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology; D014176:Protein Biosynthesis; D012160:Retina / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D017299:Rod Opsins / Q000302:isolation & purification
D003459:Crystallins; D017299:Rod Opsins; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D005346:Fibroblast Growth Factors