title: cis-acting elements in 5'-flanking region of rat alpha-fetoprotein mediating retinoic acid responsiveness.
abstract: A distal RA responsive cis-acting element has been identified in the 5'-flanking region of the alpha-fetoprotein gene by transfection of different deletion mutants of AFP-CAT fusion gene. The retinoic acid receptor specifically binds to this RA responsive cis-acting element in mobility shift assays. Furthermore, this cis-acting element functions in exogenous TK promoter in transient cotransfection assays. This study suggests a role for the RA responsive cis-acting element in the RA induction of alpha-fetoprotein gene expression.
authors.affiliation: Department of Biochemistry, College of Medicine, University of Vermont, Burlington 05405.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: J M
authors.initials: JM
authors.lastname: Dong
authors.forename: J F
authors.initials: JF
authors.lastname: Chiu
mesh_terms: D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D051381:Rats; D018168:Receptors, Retinoic Acid / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000235:genetics*
chemical_list: D009838:Oligodeoxyribonucleotides; D018168:Receptors, Retinoic Acid; D000509:alpha-Fetoproteins; D014212:Tretinoin; D015500:Chloramphenicol O-Acetyltransferase