Target cells modulate dopamine transporter gene expression during brain development.
We have analysed the expression of the dopamine transporter (DAT) gene and compared it with that of tyrosine hydroxylase, neuronal GABA transporter and synaptic vesicle monoamine transporter genes during pre- and post-natal development of rat mesencephalic dopaminergic (DA) neurones. Our results show that DAT transcripts are not detectable until embryonic day (E) 15, whilst those of the other genes analysed are already present at E12. In vitro, the level of DAT gene transcription in mesencephalic E13 DA neurones is increased in coculture with target striatal cells. Thus striatal targets cells regulate, at the transcriptional level, a key step of dopaminergic neurotransmission during DA neurone development.
Perrone-Capano
C
C
International Institute of Genetics and Biophysics, CNR, Naples, Italy.
Tino
A
A
di Porzio
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U
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000166:cytology* / Q000254:growth & development* / Q000502:physiology; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D004298:Dopamine / Q000378:metabolism*; D050483:Dopamine Plasma Membrane Transport Proteins; D004563:Electrochemistry; D015870:Gene Expression / Q000502:physiology*; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D008636:Mesencephalon / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D016133:Polymerase Chain Reaction; D006180:Proton-Translocating ATPases / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D051381:Rats; D009435:Synaptic Transmission / Q000502:physiology; D014446:Tyrosine 3-Monooxygenase / Q000276:immunology / Q000378:metabolism
D002352:Carrier Proteins; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; C493216:Slc6a3 protein, rat; D014446:Tyrosine 3-Monooxygenase; D012194:RNA-Directed DNA Polymerase; D006180:Proton-Translocating ATPases; D004298:Dopamine