title: Chromosome analysis of human spermatozoa exposed to antineoplastic agents in vitro.
abstract: We studied in vitro the cytogenetic effects of six antineoplastic agents, bleomycin (BM), cyclophosphamide (CP), daunomycin (DM), methyl methanesulfonate (MMS), mitomycin C (MMC) and triethylenemelamine (TEM) on spermatozoa, using an interspecific in vitro fertilization system between zona-free hamster oocytes and human or bull spermatozoa. In preliminary experiments with bull spermatozoa, clastogenic effects were clearly shown with BM, DM, MMS and TEM, but not with CP and MMC. In main experiments, the effects of the first four chemicals were studied in detail with human spermatozoa. Total numbers of 585 and 512 spermatozoa were karyotyped in the control and the chemical-treated groups respectively. The incidence of spermatozoa with structural chromosome aberrations was 34.5%, 53.0%, 59.3%, and 55.6% in the BM (50 micrograms/ml, 90 min), DM (0.1 microgram/ml, 90 min), MMS (100 micrograms/ml, 120 min) and TEM (0.1 micrograms/ml, 120 min) groups respectively, each showing a significantly higher incidence than the matched controls (10.1-13.5%). Breakage-type aberrations were more frequent than exchange-type aberrations in the BM, MMS and TEM groups, while the exchange-type aberrations were more frequent in the DM group. Exchanges were mainly of the chromatid type in the DM, MMS and TEM groups, while chromosome-type exchanges occurred more frequently in the BM group. These results are discussed in relation to previous data on chemical-induced chromosome aberrations in mammalian somatic cells and in mouse spermatozoa.
authors.affiliation: Department of Biological Sciences, Asahikawa Medical College, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kamiguchi
authors.forename: H
authors.initials: H
authors.lastname: Tateno
authors.forename: Y
authors.initials: Y
authors.lastname: Iizawa
authors.forename: K
authors.initials: K
authors.lastname: Mikamo
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D001761:Bleomycin / Q000633:toxicity; D002417:Cattle; D002869:Chromosome Aberrations; D006224:Cricetinae; D003520:Cyclophosphamide / Q000633:toxicity; D003630:Daunorubicin / Q000633:toxicity; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D008741:Methyl Methanesulfonate / Q000633:toxicity; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D009865:Oocytes; D013094:Spermatozoa / Q000187:drug effects*; D014265:Triethylenemelamine / Q000633:toxicity
chemical_list: D000970:Antineoplastic Agents; D009153:Mutagens; D001761:Bleomycin; D016685:Mitomycin; D003520:Cyclophosphamide; D008741:Methyl Methanesulfonate; D014265:Triethylenemelamine; D003630:Daunorubicin