Chondroitin sulphate covalently cross-links the three polypeptide chains of inter-alpha-trypsin inhibitor.
Inter-alpha-trypsin inhibitor (ITI) is a tight complex of three different proteins: bikunin and two heavy chains H1 and H2. In order to demonstrate that the three chains are covalently linked by a chondroitin sulphate chain as previously proposed [Enghild, J. J., Salvesen, G., Hefta, S. A., Thogersen, I. B., Rutherford, S. and Pizzo, S. V. (1991) J. Biol. Chem. 266, 747-751], ITI was extensively digested with thermolysin and the glycosaminoglycan-containing fragment was isolated from the digest by ion-exchange chromatography. Its peptide structural determination and mass spectrometry analysis both provide evidence that the different peptide chains constituting ITI are associated by the new cross-link described as the protein-glycosaminoglycan-protein cross-link.
Morelle
W
W
Laboratoire de Biochimie, Faculté de Pharmacie, Lille, France.
Capon
C
C
Balduyck
M
M
Sautiere
P
P
Kouach
M
M
Michalski
C
C
Fournet
B
B
Mizon
J
J
D000510:Alpha-Globulins / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D001665:Binding Sites; D002240:Carbohydrate Sequence; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000737:chemistry; D006801:Humans; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D014360:Trypsin Inhibitor, Kunitz Soybean; D014361:Trypsin Inhibitors / Q000737:chemistry / Q000378:metabolism*
D000510:Alpha-Globulins; D003432:Cross-Linking Reagents; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D010455:Peptides; C109148:SPINT2 protein, human; D014361:Trypsin Inhibitors; C011877:inter-alpha-inhibitor; D002809:Chondroitin Sulfates; D014360:Trypsin Inhibitor, Kunitz Soybean