title: Cellular and hormonal mechanisms associated with malignant bone resorption.
abstract: BACKGROUND
  To obtain a better understanding of the cellular and hormonal mechanisms responsible for the malignant bone resorption associated with metastatic carcinoma, we sought to identify whether tumor cells or tumor infiltrating macrophages were capable of lacunar bone resorption.
  
  
  METHODS
  Tumor cells and tumor-infiltrating macrophages (TIMS), (nonspecific esterase and F4/80 positive: cytokeratin and tartrate-resistant acid phosphatase and calcitonin response negative), were isolated from carcinomas that developed after subcutaneous implantation of human breast, colon, and cervical carcinoma cell lines into MFI athymic nude mice. These cells were cultured alone or with stromal cells on bone slices and evidence of lacunar resorption sought by scanning electron microscopy.
  
  
  RESULTS
  After 7 to 14 days in co-culture with UMR106 osteoblast-like cells in the presence of 1,25-dihydroxy vitamin D3, only cells of the TIM population differentiated into osteoclast-like cells (nonspecific esterase-negative: tartrate-resistant acid phosphatase-positive) capable of extensive lacunar bone resorption.
  
  
  CONCLUSIONS
  Cells within the TIM population but not tumor cells are capable of differentiation into osteoclast-like cells which can resorb bone extensively. Both 1,25 dihydroxyvitamin D3 and bone stromal cells are necessary for this to occur. TIM differentiation into cells capable of lacunar resorption could account for a component of the extensive osteolysis associated with carcinomatous skeletal metastases.
authors.affiliation: University of Oxford, Nuffield Department of Pathology and Bacteriology, John Radcliffe Hospital.
authors.forename: J M
authors.initials: JM
authors.lastname: Quinn
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumura
authors.forename: D
authors.initials: D
authors.lastname: Tarin
authors.forename: J O
authors.initials: JO
authors.lastname: McGee
authors.forename: N A
authors.initials: NA
authors.lastname: Athanasou
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D000818:Animals; D001862:Bone Resorption / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D002116:Calcitonin / Q000032:analysis / Q000378:metabolism; D002117:Calcitriol / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D002454:Cell Differentiation; D002478:Cells, Cultured; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D008264:Macrophages / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D009368:Neoplasm Transplantation; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D010010:Osteoclasts / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology
chemical_list: D007633:Keratins; D002116:Calcitonin; D002117:Calcitriol