title: Immunostaining for Leu-7 in the diagnosis of thyroid carcinoma.
abstract: The morphologic distinction between benign and malignant thyroid lesions can be difficult. Immunostaining for the Leu-7 antigen has been reported to be useful in the diagnosis of thyroid carcinoma. To test the specificity of immunostaining for Leu-7 in the diagnosis of thyroid carcinoma, we studied formalin-fixed, paraffin-embedded tissue specimens obtained from 66 thyroid lesions using the HNK-1 monoclonal antibody to Leu-7 and an avidin-biotin-complex technique. Positive reactivity for Leu-7 was seen in 11 (33%) of 33 benign thyroid conditions and in 27 (82%) of 33 thyroid carcinomas. We conclude that immunostaining for Leu-7 is not specific in the diagnosis of thyroid carcinoma.
authors.affiliation: Department of Pathology, University of Missouri Medical School, Columbia.
authors.forename: T S
authors.initials: TS
authors.lastname: Loy
authors.forename: G V
authors.initials: GV
authors.lastname: Darkow
authors.forename: L E
authors.initials: LE
authors.lastname: Spollen
authors.forename: A A
authors.initials: AA
authors.lastname: Diaz-Arias
mesh_terms: D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000378:metabolism*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism*; D001360:Avidin; D001710:Biotin; D018959:CD57 Antigens; D002277:Carcinoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007158:Immunologic Techniques; D013194:Staining and Labeling; D013964:Thyroid Neoplasms / Q000378:metabolism / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018959:CD57 Antigens; D001360:Avidin; D001710:Biotin