Standardization of immunoassays for antiphospholipid antibodies with beta 2GPI and role of other phospholipid cofactors.
Presence of beta 2 Glycoprotein I (beta 2GPI), in addition to phospholipids, is an absolute requirement for binding APA. This binding is frequently observed with beta 2GPI coated alone, however many APA react only with beta 2GPI complexed to phospholipids, but not with phospholipids alone. We demonstrate that a subgroup of rabbit polyclonal antibodies to human beta 2GPI binds to this protein only when it is coated on a solid surface, but not if it is in solution. In addition, beta 2GPI present in goat serum is strongly fixed by the coated phospholipids and the complexes formed bind as well APA as the rabbit antibodies to beta 2GPI. The diluent used for testing APA, has a strong incidence on APA's reactivity as it can be a source of beta 2GPI. Antibody binding to beta 2GPI, Prothrombin, Protein S, and Annexin V, coated in the presence or in the absence of phospholipids, was tested in 55 patients with the antiphospholipid syndrome. The strongest binding of antibodies was observed in 39 plasma to a mixture of phospholipids and purified human beta 2GPI, however 17 samples also presented a significant reactivity to beta 2GPI alone. Nine plasmas contained antibodies to Prothrombin, 4 to Protein S, 3 to Annexin V, and 1 to Protein C. We conclude that most of the APA are directed to a complex of beta 2GPI and phospholipids although in some patients antibodies to beta 2GPI alone or to other phospholipid binding proteins are present.
Amiral
J
J
Serbio Research Laboratory, Stago Group, Gennevilliers, France.
Larrivaz
I
I
Cluzeau
D
D
Adam
M
M
D000818:Animals; D017304:Annexin A5 / Q000276:immunology / Q000378:metabolism; D017152:Antibodies, Antiphospholipid / Q000032:analysis*; D000918:Antibody Specificity; D016736:Antiphospholipid Syndrome / Q000097:blood / Q000276:immunology; D001327:Autoimmune Diseases / Q000097:blood / Q000276:immunology; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards*; D006023:Glycoproteins / Q000378:metabolism*; D006041:Goats / Q000097:blood; D006801:Humans; D009185:Myelin Proteins / Q000378:metabolism; D018991:Myelin Proteolipid Protein; D011485:Protein Binding; D011486:Protein C / Q000276:immunology / Q000378:metabolism; D017293:Protein S / Q000276:immunology / Q000378:metabolism; D011516:Prothrombin / Q000276:immunology / Q000378:metabolism; D011817:Rabbits; D012015:Reference Standards; D012710:Serum Albumin, Bovine / Q000378:metabolism; D053482:beta 2-Glycoprotein I
D017304:Annexin A5; D017152:Antibodies, Antiphospholipid; D006023:Glycoproteins; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D011486:Protein C; D017293:Protein S; D053482:beta 2-Glycoprotein I; D012710:Serum Albumin, Bovine; D011516:Prothrombin