Halophilic protein stabilization by the mild solubilizing agents nondetergent sulfobetaines.
In this work, experiments performed on pig heart and halophilic malate dehydrogenase as well as halophilic elongation factor Tu demonstrate a protein stabilization property from the recently described mild solubilizing agents nondetergent sulfobetaines. A practical application is given by the separation of halophilic bacteria elongation factor Tu and halophilic malate dehydrogenase by high-performance ion-exchange chromatography achieved at reduced salt levels without significant loss of activity.
Vuillard
L
L
Institut Laue-Langevin, Grenoble, France.
Madern
D
D
Franzetti
B
B
Rabilloud
T
T
D000818:Animals; D001622:Betaine / Q000494:pharmacology*; D004355:Drug Stability; D004795:Enzyme Stability; D008291:Malate Dehydrogenase / Q000737:chemistry*; D010444:Peptide Elongation Factor Tu / Q000737:chemistry*; D013552:Swine
D001622:Betaine; D008291:Malate Dehydrogenase; D010444:Peptide Elongation Factor Tu