title: Characterization of cytokine producing T cells, TCR expression, and IgA plasma cells in salivary gland-associated tissues.
abstract: In this study, SMG, PGLN, and CLN from murine SGAT were employed in order to understand immunological characteristics of mononuclear cells that reside in these tissues for IgA responses. Based on our results, SMG harbor characteristics of IgA effector tissues. Further, PGLN has immunological features of both IgA effector tissue and systemic LN. On the other hand, CLN possesses classical characteristics of an organized systemic LN. Several important and unique features of T cells in SMG were demonstrated by this study. For example, approximately 40-50% of MC in SMG were CD3+ T cells which consisted of an equal distribution of CD4+, CD8- and CD4-, CD8+ T cell subsets. This can be considered as a unique feature of SMG since the other effector tissues usually have 2 to 3 times higher frequencies of CD4+ T cells over CD8+ T cells. Most interestingly, CD4-, CD8+ T cell subset contains both alpha beta TCR+ and gamma delta TCR+ T cells. Other than SMG, IELs possess significant number of gamma delta TCR bearing CD4-, CD8+ T cells. In terms of cytokine producing T cells, SMG contain significant numbers of CD4+ T cells which are capable of producing IL-5 and IFN-gamma. In addition, higher numbers of IL-5 producing Th 2-type cells was always seen in SMG when compared with IFN-gamma secreting Th1-type cells. Therefore, the occurrence of predominant IgA producing cells is associated with the appearance of a higher frequency of IL-5 secreting Th 2-type cells.
authors.affiliation: Department of Oral Biology, University of Alabama at Birmingham 35294, USA.
authors.forename: J
authors.initials: J
authors.lastname: Mega
authors.forename: J R
authors.initials: JR
authors.lastname: McGhee
authors.forename: H
authors.initials: H
authors.lastname: Kiyono
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells / Q000166:cytology / Q000276:immunology; D015704:CD4 Antigens / Q000378:metabolism; D016827:CD8 Antigens / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis*; D018928:Immunity, Mucosal; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D015848:Interleukin-5 / Q000096:biosynthesis; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051379:Mice; D010950:Plasma Cells / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D012469:Salivary Glands / Q000166:cytology / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D015704:CD4 Antigens; D016827:CD8 Antigens; D016207:Cytokines; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015848:Interleukin-5; D011948:Receptors, Antigen, T-Cell; D007371:Interferon-gamma