Beta subunit of DNA polymerase III holoenzyme is induced upon ultraviolet irradiation or nalidixic acid treatment of Escherichia coli.
Exposure of Escherichia coli to UV irradiation or nalidixic acid, which induce both the SOS and heat shock responses, led to a 3-4-fold increase in the amount of the beta subunit of DNA polymerase III holoenzyme, as assayed by Western blot analysis using anti-beta antibodies. Such an induction was observed also in a delta rpoH mutant lacking the heat shock-specific sigma 32 subunit of RNA polymerase, but it was not observed in recA13 or lexA3 mutants, in which the SOS response cannot be induced. Mapping of transcription initiation sites of the dnaN gene, encoding the beta subunit, using the S1 nuclease protection assay showed essentially no induction of transcription upon UV irradiation, indicating that induction is regulated primarily at the post-transcriptional level. Analysis of translational gene fusions of the dnaN gene, encoding the beta subunit, to the lacZ reporter gene showed induction of beta-galactosidase activity upon UV irradiation of cells harboring the fusion plasmids. Elimination of a 5' flanking DNA sequence in which the dnaN promoters P1 and P2 were located, did not affect the UV inducibility of the gene fusions. Thus, element(s) present from P3 downstream were sufficient for the UV induction. The induction of the dnaN-lacZ gene fusions was dependent on the recA and lexA gene products, but not on the rpoH gene product, in agreement with the immunoblot analysis. The dependence of dnaN induction on the SOS regulators was not mediated via classical repression by the LexA repressor, since the dnaN promoter does not contain a sequence homologous to the LexA binding site, and dnaN mRNA was not inducible by UV light. This suggests that SOS control may be imposed indirectly, by a post-transcriptional mechanism. The increased amount of the beta subunit is needed, most likely, for increased replication and repair activities in cells which have been exposed to UV radiation.
Tadmor
Y
Y
Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
Bergstein
M
M
Skaliter
R
R
Shwartz
H
H
Livneh
Z
Z
D001426:Bacterial Proteins / Q000235:genetics; D015153:Blotting, Western; D003001:Cloning, Molecular; D004258:DNA Polymerase III / Q000096:biosynthesis*; D004790:Enzyme Induction; D004926:Escherichia coli / Q000187:drug effects* / Q000528:radiation effects*; D015971:Gene Expression Regulation, Enzymologic; D006360:Heat-Shock Proteins / Q000235:genetics; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology*; D012323:RNA Processing, Post-Transcriptional; D011938:Rec A Recombinases / Q000235:genetics; D012697:Serine Endopeptidases; D012808:Sigma Factor / Q000235:genetics; D014157:Transcription Factors; D014466:Ultraviolet Rays; D000099160:LexA Repressor Protein
D001426:Bacterial Proteins; D004258:DNA Polymerase III; D006360:Heat-Shock Proteins; D009268:Nalidixic Acid; D011938:Rec A Recombinases; D012697:Serine Endopeptidases; D012808:Sigma Factor; D014157:Transcription Factors; D000099160:LexA Repressor Protein; C069774:heat-shock sigma factor 32