[Studies on immunoenzymatic staining technique using ultrasonicated dry egg section on PVC membrane for diagnosis of schistosomiasis japonica].
Immunoenzymatic staining technique (IEST) using PVC-membrane coated with Schistosoma japonicum ultrasonicated dry egg (UDE) and liver egg PVC-IEST was carried out to detect specific antibodies in sera from 159 cases with schistosomiasis japonica, the positive rates being 98.1% (156/159) and 97.5% (155/159), respectively. The sera from 63 healthy individuals showed false positive reaction in 2 cases and 1 case, respectively, suggesting that the sensitivity and specificity of the two tests were similar. However, the positive intensity was higher in UDE section than in liver egg section (P > 0.05). As the UDE section could be stained more obvious and the PVC membranes are cheap and could be easily brought so that this method might be applicable for practical purposes.
Gu
Q Z
QZ
Hangzhou Branch, Zhejiang Medical University.
He
J G
JG
Chen
H
H
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth; D006801:Humans; D007124:Immunoenzyme Techniques; D010063:Ovum / Q000276:immunology; D012549:Schistosoma japonicum / Q000276:immunology*; D012554:Schistosomiasis japonica / Q000175:diagnosis*; D013194:Staining and Labeling / Q000379:methods; D014465:Ultrasonics
D000909:Antibodies, Helminth; D000947:Antigens, Helminth