title: Continuous network of endoplasmic reticulum in cerebellar Purkinje neurons.
abstract: Purkinje neurons in rat cerebellar slices injected with an oil drop saturated with 1,1'-dihexadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate [DiIC16(3) or DiI] to label the endoplasmic reticulum were observed by confocal microscopy. DiI spread throughout the cell body and dendrites and into the axon. DiI spreading is due to diffusion in a continuous bilayer and is not due to membrane trafficking because it also spreads in fixed neurons. DiI stained such features of the endoplasmic reticulum as densities at branch points, reticular networks in the cell body and dendrites, nuclear envelope, spines, and aggregates formed during anoxia nuclear envelope, spines, and aggregates formed during anoxia in low extracellular Ca2+. In cultured rat hippocampal neurons, where optical conditions provide more detail, DiI labeled a clearly delineated network of endoplasmic reticulum in the cell body. We conclude that there is a continuous compartment of endoplasmic reticulum extending from the cell body throughout the dendrites. This compartment may coordinate and integrate neuronal functions.
authors.affiliation: Laboratory of Neurobiology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
authors.forename: M
authors.initials: M
authors.lastname: Terasaki
authors.forename: N T
authors.initials: NT
authors.lastname: Slater
authors.forename: A
authors.initials: A
authors.lastname: Fein
authors.forename: A
authors.initials: A
authors.lastname: Schmidek
authors.forename: T S
authors.initials: TS
authors.lastname: Reese
mesh_terms: D000818:Animals; D002232:Carbocyanines; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology*; D004058:Diffusion; D004721:Endoplasmic Reticulum / Q000648:ultrastructure*; D005456:Fluorescent Dyes; D006624:Hippocampus / Q000166:cytology; D006651:Histocytochemistry; D066298:In Vitro Techniques; D011689:Purkinje Cells / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C007392:3,3'-dihexyl-2,2'-oxacarbocyanine