Quantitative colorimetric microneutralization assay for characterization of adenoviruses.
A microneutralization assay that automates the reading and interpretation of viral infectivity and neutralization data was developed for the characterization of adenoviruses (AV). Virus, serum, and cells were added to 96-well plates, incubated for 7 days, and stained with vital stain. The A550 of solubilized dye was read in a plate reader interfaced to a personal computer which analyzed the results. Correlation of A550 values with visual observation of cytopathic effect was extremely good (r = 0.977625). Clinical isolates of 17 AV from 11 patients were characterized by colorimetric neutralization assay. Prototype AV titers tested were comparable to those determined by tube methods. Prototype homotypic antiserum titers were comparable to or greater than those determined by standard tube neutralization.
Crawford-Miksza
L K
LK
Viral and Rickettsial Disease Laboratory, California Department of Health Services, Berkeley 94704.
Schnurr
D P
DP
D000257:Adenoviridae Infections / Q000382:microbiology*; D000260:Adenoviruses, Human / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D003124:Colorimetry / Q000379:methods*; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D009500:Neutralization Tests / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods*; D013194:Staining and Labeling
D000914:Antibodies, Viral; D000956:Antigens, Viral