title: Purification and properties of a novel enzyme from Bacillus spp. T-3040, which catalyzes the conversion of dextran to cyclic isomaltooligosaccharides.
abstract: A novel enzyme, cycloisomaltooligosaccharide glucanotransferase (CITase), catalyzes the conversion of dextran to cyclic isomaltooligosaccharides by intramolecular transglucosylation (cyclization reaction). CITase was purified to homogeneity from the culture filtrate of Bacillus sp. T-3040 isolated from soil. The Mr of the enzyme was estimated to be 98,000 by SDS-PAGE. The enzyme catalyzed the cyclization reaction and gave three cyclic isomaltooligosaccharides (cycloisomalto-heptaose, -octase, and -nonaose) at a total yield of about 20%. Coupling and disproportionation reactions were also observed. These results showed that this enzyme is a multi-functional enzyme which catalyzes intramolecular and intermolecular transglucosylation.
authors.affiliation: Noda Institute for Scientific Research, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Oguma
authors.forename: K
authors.initials: K
authors.lastname: Tobe
authors.forename: M
authors.initials: M
authors.lastname: Kobayashi
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003911:Dextrans / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005964:Glucosyltransferases / Q000302:isolation & purification* / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D009844:Oligosaccharides / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D003911:Dextrans; D009844:Oligosaccharides; C021705:maltooligosaccharides; D005964:Glucosyltransferases; C087517:cycloisomaltooligosaccharide glucanotransferase