Use of inexpensive O antisera as the detecting antibodies for Salmonella antigens in the polymyxin-cloth enzyme immunoassay.
The application of O antigen-specific antisera to the detection of Salmonella lipopolysaccharide (LPS) antigens was examined in an enzyme immunoassay using polymyxin-coated polyester cloth. The LPS antigens were extracted with deoxycholate and captured on polymyxin-cloth. A mixture of rabbit antisera to Salmonella O antigens was allowed to react with the captured antigens, and the reacted antibodies were detected by an anti-rabbit IgG-peroxidase conjugate. The assay gave positive results with 40 different Salmonella serotypes which represented more than 99.9% of the serogroups isolated from over 122,000 food, feed and environmental samples analysed at Laboratory Services Division, Agriculture Canada, from 1975 to 1990. Strong cross-reactivity with Staphylococcus aureus was eliminated by pregrowth of the organisms in the presence of sodium deoxycholate. The O antisera were commercially available and are more economical than monoclonal or affinity purified polyclonal antibodies.
Blais
B W
BW
Laboratory Services Division, Agriculture Canada, Ottawa, Ontario.
Ong
H Y
HY
Yamazaki
H
H
D000907:Antibodies, Bacterial / Q000276:immunology*; D000918:Antibody Specificity; D002455:Cell Division / Q000187:drug effects; D003429:Cross Reactions; D003470:Culture Media; D003840:Deoxycholic Acid / Q000494:pharmacology; D005516:Food Microbiology; D007106:Immune Sera / Q000276:immunology; D007124:Immunoenzyme Techniques; D019081:O Antigens; D011113:Polymyxins; D011135:Polysaccharides, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D012475:Salmonella / Q000187:drug effects / Q000254:growth & development / Q000276:immunology*; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D003470:Culture Media; D007106:Immune Sera; D019081:O Antigens; D011113:Polymyxins; D011135:Polysaccharides, Bacterial; D003840:Deoxycholic Acid