Studies on calcium oxalate monohydrate crystallization: influence of inhibitors.
A simple model to study calcium oxalate monohydrate (COM) crystallization on different substrates is presented and the action of different potential inhibitors is evaluated and discussed. COM heterogeneous nucleation was assayed on solid surfaces as calcium phosphate, mixtures of mucin with calcium phosphate, and wax. In the presence of a non-protected non-renewed solid surface in contact with normal urine, COM crystal formation could be detected at short intervals (3 h). The most active heterogeneous nucleation capacity corresponded to calcium phosphate. In the presence of 10% mucin, owing to the renewal of the surface layer no COM crystal were detected on the pellet's surface. The study of citrate and pentosan polysulphate (a semisynthetic polysaccharide) on COM heterogeneous nucleation demonstrated some important inhibitory effects when concentration increased and time decreased. Maximum effects were selectively manifested on calcium phosphate surfaces. Only phytic acid at adequate concentration exhibited a total inhibitory capacity of COM formation, even during longer intervals (15 h).
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Department of Chemistry, University of the Balearic Islands, Palma de Mallorca, Spain.
Kroupa
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M
Costa-Bauzá
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A
D002129:Calcium Oxalate / Q000037:antagonists & inhibitors* / Q000737:chemistry*; D002130:Calcium Phosphates; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D003460:Crystallization; D004338:Drug Combinations; D008855:Microscopy, Electron, Scanning; D009077:Mucins; D009994:Osmolar Concentration; D010426:Pentosan Sulfuric Polyester / Q000494:pharmacology; D010833:Phytic Acid / Q000494:pharmacology; D013499:Surface Properties; D013997:Time Factors; D014885:Waxes
D002130:Calcium Phosphates; D002951:Citrates; D004338:Drug Combinations; D009077:Mucins; D014885:Waxes; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; D002129:Calcium Oxalate; D019343:Citric Acid; D010426:Pentosan Sulfuric Polyester; C485838:calcium phosphate, monobasic, anhydrous; D010833:Phytic Acid; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous