Induction of high SCEs in normal cells by 11-12 kDa plasma protein overexpressed in some sleep deprived volunteers.
The present study confirmed our earlier preliminary observation of induction of high SCEs in 1 day sleep-deprived healthy volunteers and showed that the property of induction of high SCE by after-sleep-deprived (ASD) plasma was retained both in 50% ammonium sulphate precipitates and in the dialysed plasma using a membrane of 12 kDa porocity. Further, nitrocellulose bound 11-12 kDa electrophoretically isolated plasma protein from ASD individuals was found responsible for the induction of high SCE in normal whole blood cultures. A short exposure of 3 h to 5% ASD plasma was enough to induce significantly high SCEs in normal lymphocytes which had undergone 2 replication cycles after the exposure to this factor.
Trehan
N
N
Human Genetics Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Bamezai
R
R
D000328:Adult; D001773:Blood Cells; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002478:Cells, Cultured; D006801:Humans; D008214:Lymphocytes; D008297:Male; D008970:Molecular Weight; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D012892:Sleep Deprivation / Q000502:physiology*
D001798:Blood Proteins