title: Detection of anti-human immunodeficiency virus type 1 (HIV-1) immunoglobulin G in urine by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) with recombinant reverse transcriptase as an antigen.
abstract: Anti-human immunodeficiency virus type 1 immunoglobulin G in urine was detected by an immunoassay with reverse transcriptase as the antigen and beta-D-galactosidase as the label; this immunoassay was 30-fold more sensitive than the previous immunoassay with peroxidase as the label. The sensitivity and specificity were both 100%. The lowest signal for asymptomatic carriers was 20-fold higher than the highest signal for seronegative subjects.
authors.affiliation: Department of Biochemistry, Medical College of Miyazaki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hashinaka
authors.forename: S
authors.initials: S
authors.lastname: Hashida
authors.forename: K
authors.initials: K
authors.lastname: Hirota
authors.forename: A
authors.initials: A
authors.lastname: Saitoh
authors.forename: A
authors.initials: A
authors.lastname: Nakata
authors.forename: H
authors.initials: H
authors.lastname: Shinagawa
authors.forename: S
authors.initials: S
authors.lastname: Oka
authors.forename: K
authors.initials: K
authors.lastname: Shimada
authors.forename: E
authors.initials: E
authors.lastname: Ishikawa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000941:Antigens; D005069:Evaluation Studies as Topic; D015483:HIV Antibodies / Q000652:urine*; D006679:HIV Seropositivity / Q000175:diagnosis / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data; D007074:Immunoglobulin G / Q000652:urine; D008875:Middle Aged; D012194:RNA-Directed DNA Polymerase / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D001616:beta-Galactosidase
chemical_list: D000941:Antigens; D015483:HIV Antibodies; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D012194:RNA-Directed DNA Polymerase; D001616:beta-Galactosidase