Antibody response to a Babesia bigemina rhoptry-associated protein 1 surface-exposed and neutralization-sensitive epitope in immune cattle.
Protective immunity against Babesia bigemina is hypothesized to involve antibodies directed against merozoite surface-exposed epitopes. Levels of antibody against a rhoptry-associated protein 1 (RAP-1) B-lymphocyte epitope, defined by surface-reactive and inhibitory monoclonal antibodies, in immune cattle sera were determined. All cattle produced antibodies to the epitope; however, there was limited correlation between immune protection induced by infection or RAP-1 immunization and the level of antibody to the neutralization-sensitive B-lymphocyte epitope examined.
Ushe
T C
TC
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040.
Palmer
G H
GH
Sotomayor
L
L
Figueroa
J V
JV
Buening
G M
GM
Perryman
L E
LE
McElwain
T F
TF
D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000276:immunology*; D001403:Babesia / Q000145:classification; D001404:Babesiosis / Q000276:immunology* / Q000517:prevention & control; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control; D000939:Epitopes / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology; D009500:Neutralization Tests; D015800:Protozoan Proteins / Q000276:immunology*; D013045:Species Specificity; D014611:Vaccination
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000939:Epitopes; D008565:Membrane Proteins; D015800:Protozoan Proteins; C065352:rhoptry associated protein, Plasmodium