Immunocytochemical co-localization of substance P and calcitonin gene-related peptide in afferent renal nerve soma of the rat.
Substance P, calcitonin gene-related peptide and somatostatin immunoreactivities have been demonstrated in putative afferent renal nerve fibers in the rat. Utilizing retrograde-tracing and immunohistochemistry, we labeled afferent renal nerve soma throughout dorsal root ganglia T9 to L1. Most (85%) of afferent renal nerve perikarya were immunoreactive for calcitonin gene-related peptide, 21% had substance P immunoreactivity and none had somatostatin immunoreactivity. All renal afferents immunoreactive for substance P also contained calcitonin gene-related peptide. These results provide evidence that calcitonin gene-related peptide and substance P are present and co-localized in afferent renal nerves, and therefore, mediate transmission of afferent renal input to the spinal cord in the rat.
Burg
M
M
Department of Pharmacological and Physiological Science, St. Louis University School of Medicine, MO 63104.
Zahm
D S
DS
Knuepfer
M M
MM
D000578:Amidines; D000818:Animals; D000918:Antibody Specificity; D015740:Calcitonin Gene-Related Peptide / Q000276:immunology / Q000378:metabolism*; D002232:Carbocyanines; D005456:Fluorescent Dyes; D005727:Ganglia, Spinal / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D007668:Kidney / Q000294:innervation*; D008297:Male; D009475:Neurons, Afferent / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013266:Stilbamidines; D013373:Substance P / Q000276:immunology / Q000378:metabolism*
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D000578:Amidines; D002232:Carbocyanines; D005456:Fluorescent Dyes; D013266:Stilbamidines; D013373:Substance P; C024286:3,3'-dioctadecylindocarbocyanine; C037945:diamidino yellow; D015740:Calcitonin Gene-Related Peptide