Differential expression of keratin 5 gene in non-tumorigenic and tumorigenic rat bladder cell lines.
In this study, we attempted to find a gene or genes which were differentially expressed between a non-tumorigenic rat bladder cell line and a highly tumorigenic/metastatic bladder carcinoma cell line that was derived from the former after treatment in vitro with N-methyl-N-nitrosourea. We cloned a rat keratin 5 cDNA by a differential hybridization technique and found that all of the non-tumorigenic cells (7/7) and normal bladder tissue expressed keratin 5, but most of the tumorigenic cells (8/10) did not express keratin 5. Furthermore, in a spontaneously transformed cell line, keratin 5 expression was lost during the transformation process. These results suggest that loss of keratin 5 expression is closely associated with acquisition of a tumorigenic phenotype by rat bladder non-tumorigenic cells.
Nan
L
L
Department of Pathology, Northwestern University Medical School, Chicago, IL 60611-3008.
Kawamata
H
H
Tan
X
X
Kameyama
S
S
Oyasu
R
R
D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic; D015723:Gene Library; D007633:Keratins / Q000032:analysis / Q000235:genetics*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D051381:Rats; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000737:chemistry* / Q000235:genetics*
D014408:Biomarkers, Tumor; D017931:DNA Primers; D007633:Keratins