A previously unidentified choline acetyltransferase transcript in the human foetal brain.
To identify choline acetyltransferase (ChAT) transcript(s) in human foetal brain we used the reverse polymerase chain reaction including different couples of oligonucleotide primers. The analysis indicated the presence of a new choline acetyltransferase transcript containing at its 5' end an untranslated exon (E1A) of 57 nucleotides in length extending from Nt 3771 to Nt 3828. It differs in the 5'-non coding region from the M-N-R type mRNA previously described in mouse and rat.
Schmitt
M
M
Laboratoire de génétique moléculaire des eucaryotes, CNRS, U184 INSERM, Faculté de Médecine, Strasbourg, France.
Garnier
J M
JM
Simoni
P
P
Quirin-Stricker
C
C
D001921:Brain / Q000196:embryology* / Q000201:enzymology; D002795:Choline O-Acetyltransferase / Q000235:genetics*; D005333:Fetus / Q000378:metabolism*; D006801:Humans; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D002795:Choline O-Acetyltransferase; D012194:RNA-Directed DNA Polymerase