Neuropeptides and interleukin-6 in human joint inflammation relationship between intraarticular substance P and interleukin-6 concentrations.
Plasma and synovial fluid concentrations of interleukin-6 (IL-6), using an enzyme-linked immunosorbent assay, as well as immunoreactive levels of calcitonin gene-related peptide (CGRP), substance P and vasoactive intestinal peptide (VIP) were measured in 18 patients with rheumatoid arthritis and 20 with osteoarthritis of the knee. The concentrations of IL-6 were elevated in both plasma and synovial fluids from patients with rheumatoid arthritis whereas higher levels of substance P-, CGRP- and VIP-like immunoreactivities were found in the synovial fluid, but not in plasma, from patients with rheumatoid arthritis when compared with those in osteoarthritis. Furthermore, IL-6 and substance P levels in synovial fluid were significantly correlated both in rheumatoid arthritis and osteoarthritis patients. Our data seem to support the idea of an important role shared by neuropeptides and IL-6 in the pathogenesis of human inflammatory joint disease.
Arnalich
F
F
Department of Medicine, Autonomous University, Madrid, Spain.
de Miguel
E
E
Perez-Ayala
C
C
Martinez
M
M
Vazquez
J J
JJ
Gijon-Banos
J
J
Hernanz
A
A
D001172:Arthritis, Rheumatoid / Q000097:blood / Q000378:metabolism*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D015850:Interleukin-6 / Q000378:metabolism*; D007719:Knee Joint / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009479:Neuropeptides / Q000378:metabolism*; D009994:Osmolar Concentration; D010003:Osteoarthritis / Q000097:blood / Q000378:metabolism*; D011863:Radioimmunoassay; D013373:Substance P / Q000378:metabolism*
D015850:Interleukin-6; D009479:Neuropeptides; D013373:Substance P