Induction of transcription factor AP2 mRNA expression in rat primary afferent neurons during acute inflammation.
We have examined immediate early gene mRNA expression, using in situ hybridisation in innervating dorsal root ganglion (DRG) neurons following peripheral adjuvant injection. Neuronal expression of mRNAs encoding NGFI-A (nerve growth factor-induced), NGFI-B, c-jun, jun D and jun B was undetectable in untreated controls and was unchanged following adjuvant injection. AP-2 mRNA was expressed in the majority of DRG neurons in untreated controls and was significantly increased (217 +/- 43% control) 1 h after adjuvant injection. AP2 mRNA levels returned to control values by 2 h post-injection. AP-2 may form part of the early transcriptional response that induces neuropeptide gene expression in DRG after adjuvant injection.
Donaldson
L F
LF
Department of Medicine, Western General Hospital, University of Edinburgh, UK.
McQueen
D S
DS
Seckl
J R
JR
D000208:Acute Disease; D000818:Animals; D001169:Arthritis, Experimental / Q000378:metabolism* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000235:genetics*; D005727:Ganglia, Spinal / Q000378:metabolism / Q000473:pathology; D017781:Genes, Immediate-Early; D008297:Male; D009475:Neurons, Afferent / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D050656:Transcription Factor AP-2; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D050656:Transcription Factor AP-2; D014157:Transcription Factors