Extracellular levels of serotonin in the medial pontine reticular formation in acute decerebrate cats with a microdialysis technique.
Extracellular levels of serotonin (5-HT) and 5-hydroxyindolacetic acid (5-HIAA) were measured in the medial pontine reticular formation of acute decerebrate cats. The mean basal levels of 5-HT and 5-HIAA were 26 fmol/20 microliters and 15 pmol/20 microliters. Perfusion of the dialysis probe with high K+ and Ca(2+)-free Ringer solution for 60 min resulted in 4.8-8.5 x increase and 25-48% decrease in the extracellular levels of 5-HT, respectively, in comparison to the basal 5-HT levels. Perfusion with TTX-added Ringer solution for 60 min resulted in a consistent decrease in the extracellular levels of 5-HT.
Iwakiri
H
H
National Institute for Physiological Sciences, Okazaki, Japan.
Takakusaki
K
K
Nonaka
S
S
Mori
S
S
D000818:Animals; D002118:Calcium / Q000502:physiology; D002415:Cats; D003655:Decerebrate State / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D005110:Extracellular Space / Q000737:chemistry; D005260:Female; D006719:Homovanillic Acid / Q000032:analysis; D006897:Hydroxyindoleacetic Acid / Q000032:analysis*; D008297:Male; D017551:Microdialysis; D010477:Perfusion; D011188:Potassium / Q000494:pharmacology; D012154:Reticular Formation / Q000737:chemistry*; D012701:Serotonin / Q000032:analysis*; D015222:Sodium Channels / Q000187:drug effects; D013779:Tetrodotoxin / Q000494:pharmacology
D015222:Sodium Channels; D012701:Serotonin; D013779:Tetrodotoxin; D006897:Hydroxyindoleacetic Acid; D004492:Edetic Acid; D011188:Potassium; D002118:Calcium; D006719:Homovanillic Acid