Three-dimensional solution structure of the extracellular region of the complement regulatory protein CD59, a new cell-surface protein domain related to snake venom neurotoxins.
The cell surface antigen CD59 is an inhibitor of complement-mediated lysis and a member of the Ly6 superfamily (Ly6SF) of cysteine-rich cell-surface molecules whose sequences are related to those of snake venom neurotoxins. The three-dimensional solution structure of a recombinant form of the extracellular region of the molecule (residues 1-70 of the mature protein; sCD59) has been solved by 2D NMR methods. sCD59 is a relatively flat, disk-shaped molecule consisting of a two-standed beta-sheet finger loosely packed against a protein core formed by a three-stranded beta-sheet and a short helix. Structure calculations allowed an unambiguous assignment of the disulfide-bonded cysteine pairs as 3-26, 6-13, 19-39, 45-63, and 64-69. The topology of sCD59 is similar to that of the snake venom neurotoxins and consistent with an evolutionary relationship existing between the Ly6SF and the neurotoxins.
Kieffer
B
B
Department of Biochemistry, University of Oxford, U.K.
Driscoll
P C
PC
Campbell
I D
ID
Willis
A C
AC
van der Merwe
P A
PA
Davis
S J
SJ
D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000737:chemistry*; D018957:CD59 Antigens; D003460:Crystallization; D004220:Disulfides / Q000737:chemistry; D004563:Electrochemistry; D005110:Extracellular Space / Q000737:chemistry; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009498:Neurotoxins / Q000737:chemistry*; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry; D012910:Snake Venoms / Q000737:chemistry*; D012996:Solutions
D015703:Antigens, CD; D018957:CD59 Antigens; D004220:Disulfides; D008562:Membrane Glycoproteins; D009498:Neurotoxins; D011994:Recombinant Proteins; D012910:Snake Venoms; D012996:Solutions