title: Lack of binding of peptides carrying the human platelet antigen 1 (HPA-1) dimorphism to purified HLA-DRw52a molecules.
abstract: The strong association between anti-HPA-1a alloimmunization and DR3, DRw52a phenotype in HPA-1b homozygous women suggests that these class II molecules play a crucial role in the immune response against HPA-1a. The diallelic system HPA-1 results in a single amino acid polymorphism at the residue 33 of the glycoprotein IIIa. So, we tested the binding of peptides from the 25-42 region of the GPIIIa to purified HLA-DR3 and -DRw52a molecules, using a solid phase assay and a liquid phase peptide binding assay. No binding was demonstrated, indicating that either the crucial region for binding to class II molecules is not the 25-42 region, or that other events only occurring "in vivo" are required for binding. These results may also suggest an indirect role of the residue 33 for T-cell stimulation.
authors.affiliation: Centre Régional de Transfusion Sanguine, Nantes.
authors.forename: V
authors.initials: V
authors.lastname: Braud
authors.forename: N
authors.initials: N
authors.lastname: Valentin
authors.forename: J
authors.initials: J
authors.lastname: Choppin
authors.forename: A
authors.initials: A
authors.lastname: Cesbron
authors.forename: J D
authors.initials: JD
authors.lastname: Bignon
authors.forename: D
authors.initials: D
authors.lastname: Blanchard
authors.forename: J Y
authors.initials: JY
authors.lastname: Muller
mesh_terms: D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D016824:Antigens, Human Platelet / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D018955:CD36 Antigens; D002850:Chromatography, Gel; D005260:Female; D006684:HLA-DR Antigens / Q000302:isolation & purification / Q000378:metabolism*; D059810:HLA-DR Serological Subtypes; D006801:Humans; D007114:Immunization; D007231:Infant, Newborn; D039661:Integrin beta3; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000378:metabolism*; D010641:Phenotype; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011485:Protein Binding; D013601:T-Lymphocytes / Q000276:immunology; D013921:Thrombocytopenia / Q000151:congenital / Q000453:epidemiology
chemical_list: D015703:Antigens, CD; D016824:Antigens, Human Platelet; D018955:CD36 Antigens; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C051104:HLA-DR52 antigen; C061248:ITGB3 protein, human; D039661:Integrin beta3; D010446:Peptide Fragments; C083451:human platelet antigen 1b