Inhibitory effect of interleukin-2 on histamine release from rat mast cells.
Interleukin-2 (IL-2) inhibited histamine release from rat mast cells induced by compound 48/80 in a concentration-dependent manner. The inhibitory effect of IL-2 on histamine release was also dependent on the length of the incubation period; the maximum inhibition was achieved at 8 h after IL-2 addition. Furthermore, IL-2 inhibited not only IP3 production but also 45Ca uptake in mast cells stimulated by compound 48/80. Since IL-2 enhanced [3H]-leucine uptake into mast cells, this suggests that protein synthesis may be related in some way with the inhibition of histamine release. IL-2 treatment augmented the synthesis of a protein having a molecular weight of approximately 35 kDa. From Western blotting analysis, it became clear that the production of lipocortin-I was augmented in rat mast cells by IL-2 treatment. The present study shows that IL-2 induces the synthesis of lipocortin-I in mast cells and that lipocortin-I may play some role in inhibiting histamine release from mast cells.
Tasaka
K
K
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Hamada
M
M
Mio
M
M
D000818:Animals; D017305:Annexin A1 / Q000096:biosynthesis; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D016207:Cytokines / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D017305:Annexin A1; D016207:Cytokines; D007376:Interleukin-2; D003189:p-Methoxy-N-methylphenethylamine; D002118:Calcium