Detection of anti-human immunodeficiency virus type 1 (HIV-1) immunoglobulin G in urine by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) with recombinant reverse transcriptase as an antigen.
Anti-human immunodeficiency virus type 1 immunoglobulin G in urine was detected by an immunoassay with reverse transcriptase as the antigen and beta-D-galactosidase as the label; this immunoassay was 30-fold more sensitive than the previous immunoassay with peroxidase as the label. The sensitivity and specificity were both 100%. The lowest signal for asymptomatic carriers was 20-fold higher than the highest signal for seronegative subjects.
Hashinaka
K
K
Department of Biochemistry, Medical College of Miyazaki, Japan.
Hashida
S
S
Hirota
K
K
Saitoh
A
A
Nakata
A
A
Shinagawa
H
H
Oka
S
S
Shimada
K
K
Ishikawa
E
E
D000293:Adolescent; D000328:Adult; D000368:Aged; D000941:Antigens; D005069:Evaluation Studies as Topic; D015483:HIV Antibodies / Q000652:urine*; D006679:HIV Seropositivity / Q000175:diagnosis / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data; D007074:Immunoglobulin G / Q000652:urine; D008875:Middle Aged; D012194:RNA-Directed DNA Polymerase / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D001616:beta-Galactosidase
D000941:Antigens; D015483:HIV Antibodies; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D012194:RNA-Directed DNA Polymerase; D001616:beta-Galactosidase