title: Localization of four antigenic sites involved in Venezuelan equine encephalomyelitis virus protection.
abstract: Stable neutralization and protection escape variants of a virulent strain (Trinidad Donkey) of the VEE virus were selected by monoclonal antibodies (MAbs). Determination of nucleotide sequences of nine variants revealed a clustering of single mutations in four regions of the E1 and E2 glycoproteins. Involvement of amino acid residues 206 (site E1-1), 57 and 59 (site E2-2), 180, 182, 213, 214 and 216 (site E2-6) and 232 (site E2-3) in protective epitopes was demonstrated.
authors.affiliation: Institute of Molecular Biology, State Research Center of Virology and Biotechnology Vector, Koltsovo, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Agapov
authors.forename: I A
authors.initials: IA
authors.lastname: Razumov
authors.forename: I V
authors.initials: IV
authors.lastname: Frolov
authors.forename: A A
authors.initials: AA
authors.lastname: Kolykhalov
authors.forename: S V
authors.initials: SV
authors.lastname: Netesov
authors.forename: V B
authors.initials: VB
authors.lastname: Loktev
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D002522:Chlorocebus aethiops; D003062:Codon / Q000235:genetics; D004666:Encephalitis Virus, Venezuelan Equine / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D000939:Epitopes / Q000032:analysis*; D014644:Genetic Variation; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D017354:Point Mutation; D014709:Vero Cells; D014774:Virulence
chemical_list: D000911:Antibodies, Monoclonal; D003062:Codon; D000939:Epitopes