Potentiation of bleomycin by the aminothiol WR-1065 in assays for chromosomal damage in G0 human lymphocytes.
The aminothiol radioprotector WR-1065 potentiates the induction of chromosome aberrations and micronuclei by the chemotherapy drug bleomycin in G(0) human lymphocytes. Potentiation by 5 mM WR-1065 was observed at bleomycin concentrations from 0.1 to 100 micrograms/ml in a 2-h treatment. The frequencies of micronuclei induced by bleomycin in the presence of WR-1065 reached that of 500-fold higher concentrations of bleomycin alone. The potential therapeutic implications of these findings are discussed.
Hoffmann
G R
GR
Department of Biology, College of the Holy Cross, Worcester, MA 01610.
Sayer
A M
AM
Littlefield
L G
LG
D000328:Adult; D001761:Bleomycin / Q000633:toxicity*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D004249:DNA Damage; D004357:Drug Synergism; D006801:Humans; D007399:Interphase; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D008624:Mercaptoethylamines / Q000633:toxicity*; D015162:Micronucleus Tests; D011837:Radiation-Protective Agents / Q000633:toxicity*
D008624:Mercaptoethylamines; D011837:Radiation-Protective Agents; C020174:N-(2-mercaptoethyl)-1,3-diaminopropane; D001761:Bleomycin