title: Z-RNA. The synthesis of 2'-O-[13C]methyl- and 5-methyl-analogs of ribo-CGCGCG.
abstract: Chemical synthesis of 2'-O-[13C]methyl-rCGCGCG and 5-methyl-rCGCGCG using support-aided phosphoramidite method is presented. 2'-O-Methyl guanosine derivative was separated from its 3'-O-methyl counterpart using transient 5',3'-O-silylation with 1,3-dichloro-1,1,3,3-tetraisopropyldisiloxane (Markiewicz reagent). The hexamers were obtained in a purity suitable for NMR studies.
authors.affiliation: Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.
authors.forename: E
authors.initials: E
authors.lastname: Biała
authors.forename: J
authors.initials: J
authors.lastname: Milecki
authors.forename: A
authors.initials: A
authors.lastname: Kowalewski
authors.forename: M
authors.initials: M
authors.lastname: Popenda
authors.forename: W Z
authors.initials: WZ
authors.lastname: Antkowiak
authors.forename: R W
authors.initials: RW
authors.lastname: Adamiak
mesh_terms: D001483:Base Sequence; D002247:Carbon Isotopes; D008722:Methods; D008745:Methylation; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D009843:Oligoribonucleotides; D012313:RNA