Transient mitochondrial transcript level decay in oxidative stressed chondrocytes.
Steady-state levels of 12S rRNA and NADH dehydrogenase subunit 4 mRNA (ND4) mitochondrial transcripts were measured on rabbit articular chondrocyte in culture. In pseudosynchronized chondrocytes, changes of mitochondrial RNA levels were observed during the progression of the cells in the cell cycle. Oxidative stress generated by the hypoxanthine-xanthine oxidase system (HX-XO) induced a transient decrease in the levels of both ND4 and 12S rRNA. Mitochondrial RNA levels recovered 24 h after the oxidative stress. These RNA level changes were not associated with modifications in the structure or the copy number of the mitochondrial genome. Furthermore, the decrease in the amount of the mitochondrial transcripts observed may be related to a transient inhibition of mitochondrial transcription since the treatment of cells with ethidium bromide (a mitochondrial transcription inhibitor) resulted in the same decrease in 12S rRNA level as HX-XO treatment alone.
Vincent
F
F
Laboratoire de Pharmacologie Cellulaire de l'E.P.H.E., Centre de Recherches Biomédicales des Cordeliers, Paris, France.
Corral-Debrinski
M
M
Adolphe
M
M
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002453:Cell Cycle; D002478:Cells, Cultured; D004249:DNA Damage; D004272:DNA, Mitochondrial / Q000378:metabolism; D019271:Hypoxanthine; D007042:Hypoxanthines / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism; D010084:Oxidation-Reduction; D012313:RNA / Q000378:metabolism*; D000077278:RNA, Mitochondrial; D011817:Rabbits; D013312:Stress, Physiological / Q000378:metabolism*; D013997:Time Factors; D014969:Xanthine Oxidase / Q000494:pharmacology
D004272:DNA, Mitochondrial; D007042:Hypoxanthines; D000077278:RNA, Mitochondrial; D019271:Hypoxanthine; D012313:RNA; D014969:Xanthine Oxidase