Monoclonal antibodies reactive with specific amino acid sequences of the 126 K protein of tobacco mosaic virus.
The 126 K protein of tobacco mosaic virus (TMV) is an NTP binding protein that has guanylyl transferase activity and is predicted to be an ATPase/helicase. In this paper we report the generation of monoclonal antibodies (Mabs) that react with specific amino acid sequences of the 126K protein. The Mabs were generated after immunizing mice with a partially purified preparation of the 126 K protein (native) obtained by centrifugal fractionation of the infected tissue extracts. The Mabs were assayed for specific reactivity by western blotting and by their reactivity with non-overlapping decapeptides corresponding to the entire amino acid sequence of the 126 K protein of TMV. A total of 11 Mabs reactive with specific peptides and three other Mabs that did not react with any peptide but reacted with the 126 K protein were identified.
Das
P
P
Department of Biological Sciences, Wayne State University, Detroit, Michigan.
Hari
V
V
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D011817:Rabbits / Q000276:immunology; D014026:Nicotiana / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000276:immunology*; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology*
C071899:130k protein, Tobacco mosaic virus; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes; D014764:Viral Proteins