Encephalitogenicity of myelin basic protein exon-2 peptide in mice.
Immunization with a synthetic peptide with an amino acid sequence corresponding to mouse myelin basic protein exon-2 induced mild experimental allergic encephalitis (EAE) in B10.RIII mice, very mild disease in SJL/J mice and no disease in (SJL x PL)F1 hybrid mice. In contrast, adoptive transfer of an exon-2 peptide-specific T cell line from SJL mice induced severe relapsing EAE in syngeneic recipients. The T cell line was specific for exon-2 peptide and did not cross-react appreciably with an MBP preparation consisting of the 18.5 and 14-kDa isoforms. mRNA for exon-2 containing isoforms could be demonstrated in the spinal cord of SJL/J and B10.RIII mice by amplification using exon-2 and exon-4 oligonucleotide primers. On a relative basis, the level of exon-2 cDNA was lower than that of exon-1 cDNA in the same spinal cord preparations from both strains of mice.
Fritz
R B
RB
Department of Microbiology, Medical College of Wisconsin, Milwaukee 53226.
Zhao
M L
ML
D000595:Amino Acid Sequence; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D000939:Epitopes; D005091:Exons; D006824:Hybridization, Genetic; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000235:genetics* / Q000276:immunology*; D010446:Peptide Fragments / Q000235:genetics* / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014158:Transcription, Genetic
D018076:DNA, Complementary; D000939:Epitopes; D004676:Myelin Basic Protein; D010446:Peptide Fragments