Naphthalene black staining of granules of eosinophilic granulocytes: proposed mechanism of action using chemical blockade and computer graphics.
Histochemical staining of the granules of eosinophilic granulocytes and subsequent blockade of the reaction by alkaline benzyl was strongly suggestive that in its purified form, the diazo dye naphthalene black reacts with tissue sites containing high concentrations of arginine residues. Computer graphics modelling indicated that the sulfonate group of the dye reacts electrostatically with the guanidino functional group of arginine. This acid-base type reaction likely has a stoichiometric ratio of 2:1 in favor of the amino acid.
Cairns
D
D
University of Sunderland, School of Health Sciences, United Kingdom.
Hay
J
J
D000580:Amido Black; D001120:Arginine / Q000032:analysis; D003196:Computer Graphics; D003594:Cytoplasmic Granules / Q000737:chemistry; D004801:Eosine Yellowish-(YS); D006098:Granulocytes / Q000737:chemistry* / Q000648:ultrastructure; D006651:Histocytochemistry; D006801:Humans; D008954:Models, Biological; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods
D001120:Arginine; D000580:Amido Black; D004801:Eosine Yellowish-(YS)