Identification of the GABAA receptor subtype mRNA in human pancreatic tissue.
Evidence suggests a physiological role of the GABAA receptor in the pancreas. Clinically, an autoimmune reaction involving the GABA biosynthesizing enzyme, glutamic acid decarboxylase has been implicated in the development of insulin-dependent diabetes mellitus. To determine the subtypes of GABAA receptor expressed in human pancreas, we analyzed, with the use of the reverse-transcription/polymerase chain reaction technique human pancreatic tissue for the presence of GABAA receptor subunits alpha 1-6, beta 1-3, and gamma 1-2 transcripts. Unlike brain tissue, pancreatic tissue only expresses the alpha 2, beta 3 and gamma 1 subunits. Our results provide evidence of a specific GABAA receptor subtype expressed in human pancreatic tissue.
Yang
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W
Department of Molecular Biology, CoCensys Inc., Irvine, CA 92718.
Reyes
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AA
Lan
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D001483:Base Sequence; D001569:Benzodiazepines / Q000494:pharmacology; D002540:Cerebral Cortex / Q000737:chemistry; D003001:Cloning, Molecular; D004357:Drug Synergism; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010179:Pancreas / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase; D018079:Receptors, GABA / Q000737:chemistry / Q000187:drug effects / Q000235:genetics*; D017421:Sequence Analysis; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D046911:Macromolecular Substances; D012333:RNA, Messenger; D018079:Receptors, GABA; D001569:Benzodiazepines; D005680:gamma-Aminobutyric Acid; D012313:RNA; D012194:RNA-Directed DNA Polymerase