HIV-1 gp41 shares a common immunologic determinant with human T, B and monocyte cell lines.
Several examples of molecular mimicry between HIV-1 and human proteins are reported in the literature. Here we report on yet another example. The monoclonal antibody 3D6 recognized a 17-amino-acid region in HIV-1 gp41 (amino acids 602-618) and could bind to the human T-cell lines H9 and Molt4, B cell lines Raji and Bjab, and monocyte cell lines U937 and HL60. By Western blot using 3D6, a strong band of 43 kDa and a very weak band of 80 kDa were detected in lysates of H9, Molt4, Raji and Bjab cell lines, but only a strong band of 43 kDa was observed in case of U937 and HL60 cells, under reducing or non-reducing conditions. The results indicate the presence of a common immunologic determinant between HIV-1 gp41 and membrane proteins of these human T, B and monocyte cells.
Chen
Y H
YH
Ludwig-Boltzmann-Institute for AIDS-Research, Innsbruck, Austria.
Susanna
A
A
Böck
G
G
Steindl
F
F
Katinger
H
H
Dierich
M P
MP
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D015700:HIV Envelope Protein gp41