One-hour downward capillary blotting of RNA at neutral pH.
This report describes a setup for the downward capillary blotting of RNA with the use of 10 x SSC as a transfer solution. The setup is composed of a stack of blotting papers, hybridization membrane, and agarose gel. Two layers of blotting paper connect the stack with two reservoirs containing transfer solution. Using this setup, blotting of RNA fragments (< 7.5 kb) can be completed in 1 h. If necessary, the blotting time can be expanded from 1 to 18 h without decrease in hybridization efficiency of RNA.
Chomczynski
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P
Division of Endocrinology, College of Medicine, University of Cincinnati, Ohio 45267-0547.
Mackey
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K
D000199:Actins / Q000378:metabolism; D002197:Capillary Action; D018076:DNA, Complementary; D004587:Electrophoresis, Agar Gel / Q000295:instrumentation / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008751:Methylene Blue; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012313:RNA / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism; D013997:Time Factors
D000199:Actins; D018076:DNA, Complementary; D012333:RNA, Messenger; D012313:RNA; D008751:Methylene Blue