title: Isolation of a Puumala-like virus from Mus musculus captured in Yugoslavia and its association with severe hemorrhagic fever with renal syndrome.
abstract: An outbreak of severe hemorrhagic fever with renal syndrome (HFRS) occurred in 1988 in Pozarevac, Serbia, Yugoslavia. The disease was diagnosed in 4 children and 1 adult, and 1 of the children died. Rodents were captured from the same area and virus isolation attempted. A hantavirus, POZ-M1, was isolated from lung tissues of hantavirus antigen-positive Mus musculus. Serology and restriction enzyme digestion of polymerase chain reaction-amplified segments from this virus showed that it was a strain of Puumala (PUU) virus, the causative agent of nephropathia epidemica. While Clethrionomys glareolus is the major rodent host for PUU virus, these results suggest that M. musculus may also play an important role in harboring and transmitting PUU-like viruses. The serologic association of this virus with patients with severe HFRS reaffirms that PUU-like viruses may cause severe disease in addition to the generally mild form normally associated with nephropathia epidemica.
authors.affiliation: US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland.
authors.forename: G
authors.initials: G
authors.lastname: Diglisic
authors.forename: S Y
authors.initials: SY
authors.lastname: Xiao
authors.forename: A
authors.initials: A
authors.lastname: Gligic
authors.forename: M
authors.initials: M
authors.lastname: Obradovic
authors.forename: R
authors.initials: R
authors.lastname: Stojanovic
authors.forename: D
authors.initials: D
authors.lastname: Velimirovic
authors.forename: V
authors.initials: V
authors.lastname: Lukac
authors.forename: C A
authors.initials: CA
authors.lastname: Rossi
authors.forename: J W
authors.initials: JW
authors.lastname: LeDuc
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000096:biosynthesis; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D004197:Disease Reservoirs; D005260:Female; D006476:Orthohantavirus / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D006480:Hemorrhagic Fever with Renal Syndrome / Q000453:epidemiology* / Q000382:microbiology / Q000635:transmission; D006801:Humans; D008168:Lung / Q000382:microbiology; D008297:Male; D051379:Mice / Q000382:microbiology*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012367:RNA, Viral / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D011920:Rats, Inbred WF; D014709:Vero Cells; D015022:Yugoslavia / Q000453:epidemiology
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase