title: Pasteurella haemolytica leukotoxin induces histamine release from bovine pulmonary mast cells.
abstract: Pasteurella haemolytica A1 leukotoxic culture supernatant was evaluated for its ability to induce histamine release from bovine pulmonary mast cells isolated by enzymatic dispersion of lung tissue. Histamine was measured by a radioimmunoassay technique. Leukotoxic culture supernatant of P. haemolytica significantly released histamine in a time and concentration-related manner. This effect was lost when culture supernatant was heat-inactivated or preincubated with leukotoxin neutralizing rabbit serum. Preincubation of the mast cells with propranolol or p-bromophenacyl bromide reduced the histamine-releasing effect of leukotoxin, while verapamil enhanced release. Experimental infection of calves with P. haemolytica A1 reduced the total histamine content of pulmonary mast cells recovered at postmortem. Histamine release induced by P. haemolytica leukotoxin is likely an important factor in the pathogenesis of bovine pneumonic pasteurellosis.
authors.affiliation: Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph.
authors.forename: T E
authors.initials: TE
authors.lastname: Adusu
authors.forename: P D
authors.initials: PD
authors.lastname: Conlon
authors.forename: P E
authors.initials: PE
authors.lastname: Shewen
authors.forename: W D
authors.initials: WD
authors.lastname: Black
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D005098:Exotoxins / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D006854:Hydrocortisone / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008168:Lung / Q000187:drug effects* / Q000502:physiology; D016978:Mannheimia haemolytica; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology; D011433:Propranolol / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
chemical_list: D001427:Bacterial Toxins; D005098:Exotoxins; C018579:leukotoxin; D005576:Colforsin; D000001:Calcimycin; D011433:Propranolol; D014700:Verapamil; D007545:Isoproterenol; D006854:Hydrocortisone