title: Expression of myelin-associated glycoprotein isoforms after sciatic nerve crush injury in mice.
abstract: The large myelin-associated glycoprotein isoform (L-MAG) protein and small myelin-associated glycoprotein isoform (S-MAG) protein were demonstrated after sciatic nerve crush injury in mice by an immunoblotting technique using specific antibodies to the L-MAG protein and the S-MAG protein, respectively. Immunoblots indicated a rapid decrease in expression of both isoform proteins in the crushed sciatic nerves to < 10% of the control side. By 13 d after injury, L-MAG protein expression had quickly recovered to 100% of the control level. Following the increase in L-MAG protein expression, S-MAG protein expression recovered to 100% by 20 d after injury. It has been reported that the developmental maximum expression of L-MAG protein precedes that of S-MAG protein in both central and peripheral nervous system (CNS and PNS). Our previous work demonstrated that L-MAG mRNA was characteristically induced at the time of most active myelination, including remyelination in the CNS. We here have shown the expression of L-MAG protein precedes that of S-MAG protein during active remyelination in the PNS. This suggests that it plays an important role in the early stage of myelin formation.
authors.affiliation: Department of Neurology, Niigata University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Inuzuka
authors.forename: S
authors.initials: S
authors.lastname: Sato
authors.forename: N
authors.initials: N
authors.lastname: Fujita
authors.forename: H
authors.initials: H
authors.lastname: Baba
authors.forename: R
authors.initials: R
authors.lastname: Nakano
authors.forename: H
authors.initials: H
authors.lastname: Ishiguro
authors.forename: T
authors.initials: T
authors.lastname: Miyatake
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007536:Isomerism; D051379:Mice; D008815:Mice, Inbred Strains; D009185:Myelin Proteins / Q000096:biosynthesis* / Q000276:immunology; D009186:Myelin Sheath / Q000378:metabolism / Q000502:physiology; D018992:Myelin-Associated Glycoprotein; D009409:Nerve Crush; D017933:Peripheral Nervous System / Q000378:metabolism / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism*
chemical_list: D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D012333:RNA, Messenger