title: Influence of atrial natriuretic peptide on cyclic nucleotides and amylase release in rat parotid salivary gland in vitro.
abstract: Atrial natriuretic peptide (ANP), sodium nitroprusside and hydroxylamine increased cGMP accumulation in rat parotid acinar cells both in the presence and absence of forskolin but in a different manner. On the other hand, ANP decreased forskolin-stimulated cAMP accumulation, although sodium nitroprusside and hydroxylamine had no effect on cAMP accumulation. Amylase release stimulated by forskolin, dibutyryl-cAMP or isoproterenol was depressed by ANP, whereas sodium nitroprusside and hydroxylamine did not evoke the inhibition of forskolin-stimulated amylase release. These results suggest that the inhibition of cAMP accumulation and of amylase release by ANP were not mediated via cGMP produced by guanylate cyclase-A.
authors.affiliation: Department of Oral Biochemistry, Nippon Dental University, School of Dentistry, Niigata, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nashida
authors.forename: A
authors.initials: A
authors.lastname: Imai
authors.forename: H
authors.initials: H
authors.lastname: Shimomura
mesh_terms: D000681:Amylases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000502:physiology*; D003994:Bucladesine / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism* / Q000502:physiology; D004789:Enzyme Activation / Q000187:drug effects; D006162:Guanylate Cyclase / Q000378:metabolism; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000494:pharmacology; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009599:Nitroprusside / Q000494:pharmacology; D010306:Parotid Gland / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013268:Stimulation, Chemical
chemical_list: D006898:Hydroxylamines; D009599:Nitroprusside; D005576:Colforsin; D019811:Hydroxylamine; D003994:Bucladesine; D009320:Atrial Natriuretic Factor; D002217:Carbachol; D000242:Cyclic AMP; D000681:Amylases; D006162:Guanylate Cyclase; D006152:Cyclic GMP; D007545:Isoproterenol