Suppressing effects of human fetal cell extract on micronuclei induced by cyclophosphamide in mice.
The genotoxicity of human fetal cell extract (HFCE) and its effect on the frequency of micronucleated polychromatic erythrocytes (PCE-MNF) in mice induced by cyclophosphamide (CP) were studied. Statistically significant differences were not found between the control group and each group treated with HFCE (0.3, 3, 30 mg/kg bw). CP (200 mg/kg bw) induced a marked increase in MNF (P < 0.01). Administered together with CP, HFCE suppressed the increase of MNF induced by CP. The reduction effect is dependent on the dose of HFCE. At doses of 3 and 30 mg/kg bw HFCE, MNF decreased markedly (P < 0.05 and < 0.01, respectively). It showed that HFCE did not induce micronucleus formation, while it could suppress the micronucleus formation induced by CP in mice. The results suggested that HFCE might be antimutagenic and have potential value in clinical application.
Chen
S Q
SQ
Cytogenetic Laboratory, Jiangsu Institute of Cancer Research, Nanjing, People's Republic of China.
Xue
K X
KX
Ma
G J
GJ
Wu
J Z
JZ
Wang
H
H
Xiang
L P
LP
Cheng
N
N
D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002457:Cell Extracts / Q000494:pharmacology*; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors / Q000633:toxicity*; D005260:Female; D005333:Fetus / Q000166:cytology*; D006801:Humans; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*
D016587:Antimutagenic Agents; D002457:Cell Extracts; D009153:Mutagens; D003520:Cyclophosphamide