Human cytomegalovirus replication correlates with differentiation in a hematopoietic progenitor cell line and can be modulated by HIV-1.
Human cytomegalovirus (HCMV) infection of a CD34+ hematopoietic progenitor cell line (TF1) was studied before and after TPA differentiation. TF1 cells were found to be infected but the virus does not replicate, while differentiated TF1 cells can be infected and allow HCMV complete replication. In the same system we studied the interaction between HCMV and HIV and found that while contact between HIV gp 120 and the HCMV-infected cell has an inhibitory effect, exogenous Tat protein stimulates HCMV replication. The interaction between HCMV and HIV in hematopoietic progenitor cells is complex and depends on several factors that can have opposite effects.
Lazzarotto
T
T
Institute of Microbiology, University of Bologna, Italy.
Furlini
G
G
Re
M C
MC
Ramazzotti
E
E
Campisi
B
B
Landini
M P
MP
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D000956:Antigens, Viral / Q000032:analysis; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000502:physiology*; D004279:DNA, Viral / Q000032:analysis; D005455:Fluorescent Antibody Technique; D015696:Gene Products, tat / Q000378:metabolism; D016679:Genome, Viral; D016655:HIV Core Protein p24 / Q000032:analysis; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006412:Hematopoietic Stem Cells; D006801:Humans; D007700:Kinetics; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014779:Virus Replication; D054322:tat Gene Products, Human Immunodeficiency Virus
D015703:Antigens, CD; D018952:Antigens, CD34; D000956:Antigens, Viral; D004279:DNA, Viral; D015696:Gene Products, tat; D016655:HIV Core Protein p24; D015699:HIV Envelope Protein gp120; D011994:Recombinant Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus; D013755:Tetradecanoylphorbol Acetate