Human retinal pigmented epithelial cells produce nitric oxide in response to cytokines.
The present study demonstrates that human retinal pigmented epithelial cells produce nitric oxide (NO) upon co-treatment with interferon gamma (IFN gamma) and interleukin-1 beta (IL-1 beta). The biosynthesis of NO, which was measured by the accumulation of the stable end-product nitrite, requires an induction period of approximately 12 hours and continues for at least three days. The synthesis was abolished by the stereoselective inhibitors of NO synthase (NOS), NG-monomethyl-L-arginine, N omega-nitro-L-arginine and N omega-nitro-L-arginine methyl ester and by cycloheximide. Transforming growth factor beta suppressed cytokine-induced NOS. The results indicate that cytokines such as IFN gamma and IL-1 beta are capable of inducing NOS, while TGF beta prevents this induction, in subcultured pigmented epithelial cells from the human retina.
Goureau
O
O
Unité de Recherches Gérontologiques, INSERM U 118, Association Claude Bernard, Paris, France.
Hicks
D
D
Courtois
Y
Y
D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D016207:Cytokines / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D007700:Kinetics; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase; D009573:Nitrites / Q000378:metabolism; D019335:Nitroarginine; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D019323:omega-N-Methylarginine
D016207:Cytokines; D007375:Interleukin-1; D009573:Nitrites; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D016222:Fibroblast Growth Factor 2; D019335:Nitroarginine; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D007371:Interferon-gamma; D001120:Arginine; D003513:Cycloheximide; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D019331:NG-Nitroarginine Methyl Ester