T cell responses in macaques after vaginal immunization with particulate SIV p27 antigen.
Rhesus monkeys were immunized by the vaginal and oral routes using a recombinant particulate simian immunodeficiency virus (SIV) antigen. Augmenting vaginal by oral immunization in macaques elicits proliferative CD4+ T cells in the circulation which are specific to the immunizing p27 antigen. Reconstitution of enriched CD4+ T cells, B cells and macrophages from circulating mononuclear cells help B cells in specific IgA anti-p27 antibody synthesis. The results suggest that augmented vaginal immunization induces systemic CD4+ T and B cell responses which may play a part in the protective immunity against SIV (HIV) infection.
Panagiotidi
C
C
Department of Immunology, UMDS, Guy's Hospital, London, United Kingdom.
Bergmeier
L A
LA
Gearing
A J
AJ
Adams
S E
SE
Lehner
T
T
D000282:Administration, Intravaginal; D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000008:administration & dosage*; D001402:B-Lymphocytes / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D015683:Gene Products, gag / Q000008:administration & dosage* / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control / Q000635:transmission; D006801:Humans; D018928:Immunity, Mucosal; D007114:Immunization; D008213:Lymphocyte Activation; D008252:Macaca fascicularis; D008253:Macaca mulatta; D018449:SAIDS Vaccines / Q000008:administration & dosage; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014621:Vagina / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; C066594:Gag protein p27, Simian immunodeficiency virus; D015683:Gene Products, gag; D018449:SAIDS Vaccines