Evidence for a structural difference in the CD7 polypeptide on human thymocytes and intraepithelial lymphocytes defined by a new monoclonal antibody, 3D9.
Intestinal intraepithelial lymphocytes (IEL) represent a distinct subpopulation of lymphocytes located on or above the basement membrane and adjacent to the basolateral membrane of enterocytes. Thus IEL are strategically positioned to mediate a response to the uptake of foreign antigens or to the alteration of enterocytes by injury or infection. Because of their unique location, we hypothesized that IEL might selectively express specialized cell surface proteins important in their site-specific localization or function. To identify such proteins, we immunized mice with purified human IEL and identified one monoclonal antibody, 3D9, which was found to react with a majority of IEL but with very few lamina propria lymphocytes (LPL) and weakly with most peripheral blood lymphocytes (PBL). Evaluation of this antibody with two-dimensional gels demonstrated that it reacts with CD7, previously known as an early thymocyte differentiation antigen. Interestingly, unlike all other anti-CD7 monoclonal antibodies, 3D9 identified only occasional thymocytes by immunohistochemistry suggesting that CD7 is structurally different on IEL and thymocytes. However, the CD7 polypeptide immunoprecipitated from IEL and thymocytes appeared identical in SDS-PAGE mobility and in charge by two-dimensional gels, despite being recognized differently by monoclonal antibodies. These studies emphasize the expression of CD7 by IEL T cells and reveal the existence of an undefined structural difference between CD7 molecules on IEL compared to thymocytes.
Russell
G J
GJ
Department of Pediatrics, Massachusetts General Hospital, Boston.
Parker
C M
CM
Cepek
K L
KL
Brenner
M B
MB
Bhan
A K
AK
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D019011:Antigens, CD7; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D002648:Child; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology; D000939:Epitopes / Q000737:chemistry; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology*; D051379:Mice; D015394:Molecular Structure; D010455:Peptides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D019011:Antigens, CD7; D000945:Antigens, Differentiation, T-Lymphocyte; D000939:Epitopes; D010455:Peptides