title: Use of computer graphic filters for the nuclear grading of hematoxylin and eosin-stained specimens from prostatic lesions.
abstract: An inexpensive workstation is being developed to assist pathologists in diagnosing routine hematoxylin and eosin-stained slides. A linear discriminant model was applied to karyometric features of prostate lesions, and a grade according to Mostofi was determined from the discriminant values. Twenty cases, five of hyperplasia and five each of carcinoma Mostofi grades I, II and III, for a total of 600 nuclei, were selected to train the model. Computer graphic filters were constructed from the discriminant values. Each segmented nucleus has a colored frame (the graphic filter) displayed around it. The color, determined from discriminant values and correlated with the grades, ranges from green for hyperplasia, yellow for low grade, orange for medium grade and red for high grade. An additional 20 cases, 5 of hyperplasia and 5 each of the Mostofi grades, for a total of 538 nuclei, were selected to test the graphic filter. Ninety-six percent of the hyperplasia nuclei were framed in green, 84% of low grade nuclei were framed in yellow, 90% of medium grade nuclei were framed in orange, and 89% of high grade nuclei were framed in red. These results indicate the potential of the color graphic filter to show the pathologist immediate and accurate visual information about the grade of a nucleus. This method may help with the difficult diagnosis of borderline lesions and may help in making the diagnosis from scanty biopsy material.
authors.affiliation: Department of Pathology and Cell Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania 19107-5244.
authors.forename: M
authors.initials: M
authors.lastname: Bibbo
authors.forename: J
authors.initials: J
authors.lastname: Xiao
authors.forename: R
authors.initials: R
authors.lastname: Christen
authors.forename: B
authors.initials: B
authors.lastname: Fitzpatrick
authors.forename: H
authors.initials: H
authors.lastname: Galera-Davidson
authors.forename: P H
authors.initials: PH
authors.lastname: Bartels
authors.forename: C
authors.initials: C
authors.lastname: Minimo
mesh_terms: D002467:Cell Nucleus / Q000473:pathology*; D003196:Computer Graphics; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008297:Male; D011470:Prostatic Hyperplasia / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000473:pathology*
chemical_list: D004801:Eosine Yellowish-(YS); D006416:Hematoxylin