Analysis of mono- and oligosaccharide isomers derivatized with 9-aminopyrene-1,4,6-trisulfonate by capillary electrophoresis with laser-induced fluorescence.
A method of analysis of isomers of mono- and oligosaccharides derivatized with 9-aminopyrene-1,4,6-trisulfonate (APTS) by capillary electrophoresis (CE) in simple buffer systems is presented. The derivatization chemistry is performed at 75 degrees C for 1 h for most mono- and oligosaccharides. Sialyllactose was derivatized at 37 degrees C for 15 h without significant desialylation. Most of the APTS derivatized isomers of mono- and oligosaccharides are resolved in buffers such as acetate (pH 5.0), 3-(N-morpholino)-propanesulfonic acid (pH 7.0), and phosphate (pH 7.4). The mechanism of CE separation using the above buffers is based on the differences in hydrodynamic volume of the derivatized species and is different from that in borate or high pH buffer.
Chen
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Advanced Technology Center, Beckman Instruments Inc., Fullerton, California 92634, USA.
Evangelista
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D019075:Electrophoresis, Capillary; D006863:Hydrogen-Ion Concentration; D009005:Monosaccharides / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis*
D009005:Monosaccharides; D009844:Oligosaccharides