The in vitro action of FK 565, bleomycin and cyclosporin A on different cell populations, with respect to the release of GM-CSF in the mouse.
The experimental immunostimulatory tripeptide FK 565, the anti-tumor antibiotic Bleomycin and the immunosuppressive agent Cyclosporin A were examined for their in vitro effects on granulocyte-macrophage colony stimulating factor (GM-CSF) production utilizing different cell populations from three distinct murine stains. FK 565 produced an increase in GM-CSF release in all three experimental models used, providing evidence that FK 565 is among other things, a macrophage stimulating agent. Bleomycin increased GM-CSF release in the BALB/c and the 'Scid' mouse. Paradoxically with cells from the 'nude' mouse a significant decrease was observed, indicating that Bleomycin may induce a suppressive effect on B-cells. Cyclosporin A was found to inhibit the release of GM-CSF from BALB/c mice, however, no effect was observed in either 'nude' or 'Scid' mice.
Blaney
B A
BA
Department of Pathology, Royal College of Surgeons of England, London.
Hasan
S I
SI
Koe
L A
LA
Turk
J L
JL
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D016513:Mice, SCID; D009842:Oligopeptides / Q000494:pharmacology*; D013154:Spleen / Q000187:drug effects / Q000378:metabolism*
D000276:Adjuvants, Immunologic; D009842:Oligopeptides; D001761:Bleomycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D016572:Cyclosporine; C039537:heptanoyl-gamma-D-glutamyl-L-meso-diaminopimelyl-D-alanine