Interaction of hyperthermia and chemotherapy agents; cell lethality and oncogenic potential.
Hyperthermia was combined with bleomycin, melphalan and cis-platinum in order to examine cell lethality and oncogenic transformation in C3H10T1/2 cells from the adjuvant use of heat with chemotherapy agents. When cells were exposed concurrently to 42.5 degrees C and each of the three chemotherapy agents, heat enhanced both the cytotoxic and oncogenic potential of the drugs. Hyperthermia-enhanced ratios were largest for bleomycin-treated cells. Examination of transformation incidences expressed as a function of surviving fraction, i.e. the cytotoxicity of treatment and therefore drug-heat efficacy, showed that for a given level of cell killing the combination of heat and cis-platinum resulted in fewer transformants per surviving cell than for cis-platinum alone. Hyperthermia appears to reduce the oncogenic potential of low concentrations of melphalan but has no effect on bleomycin-induced oncogenic transformation.
Miller
R C
RC
Center for Radiological Research, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
Richards
M
M
Baird
C
C
Martin
S
S
Hall
E J
EJ
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000633:toxicity; D001761:Bleomycin / Q000633:toxicity; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002945:Cisplatin / Q000633:toxicity; D003131:Combined Modality Therapy; D006358:Hot Temperature; D006801:Humans; D006979:Hyperthermia, Induced; D008558:Melphalan / Q000633:toxicity; D051379:Mice; D009369:Neoplasms / Q000188:drug therapy / Q000628:therapy
D000970:Antineoplastic Agents; D001761:Bleomycin; D002945:Cisplatin; D008558:Melphalan