Cloning of FRK, a novel human intracellular SRC-like tyrosine kinase-encoding gene.
We report the cloning of a novel tyrosine kinase (TyK)-encoding gene (TYK) from the human hepatoma cell line Hep3B. Using the polymerase chain reaction (PCR) and oligodeoxyribonucleotide primers based on conserved TYK motifs, a 180-bp fragment was cloned and used to obtain full-length cDNA clones of 2.9 kb, with an open reading frame of 505 amino acids (aa). Restricted expression was detected by Northern blotting or reverse-transcribed PCR in a broad range of cell lines. The predicted aa sequence contains characteristic TyK motifs without a transmembrane region, suggesting an intracellular localization. There was 49% aa sequence identity with human FYN product and 47% with human SRC product; however, several structural differences distinguish this clone from other SRC subfamily members. This clone, FYN-related kinase or FRK, is a novel member of the intracellular TYK gene family.
Lee
J
J
Genentech Inc., South San Francisco, CA 94080.
Wang
Z
Z
Luoh
S M
SM
Wood
W I
WI
Scadden
D T
DT
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000378:metabolism; D016391:Genes, src; D006801:Humans; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D005810:Multigene Family; D009363:Neoplasm Proteins; D011061:Poly A / Q000032:analysis; D016133:Polymerase Chain Reaction; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics*; D012313:RNA / Q000032:analysis; D012333:RNA, Messenger; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D018076:DNA, Complementary; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA; D011505:Protein-Tyrosine Kinases; C092830:FRK protein, human