Signal transduction in T lymphocytes of SLE patients: lectin-activated phosphorylation on tyrosine.
A comparative study of signal transduction through tyrosine phosphorylation process in peripheral blood lymphocytes from SLE patients and healthy subjects reveal some modifications in the phosphorylation pattern of SLE T lymphocytes. Thus, the level of constitutive tyrosine phosphorylation in resting SLE T lymphocytes is higher than in lymphocytes from healthy subjects. In SLE T lymphocytes, a cellular proteic substrate with an apparent molecular weight of about 37 kDa is constitutively phosphorylated. Some differences in the pattern of phosphorylation are obvious in lectin (Con A, PHA)-activated T lymphocytes. Thus, Con A activation enhances the phosphorylation of cellular substrates with molecular weight in the range of 55-80 kDa from SLE T lymphocytes. Moreover, the 21 kDa substrate is also hyperphosphorylated after PHA activation of SLE lymphocytes.
Matache
C
C
Cantacuzino Institute, Bucharest, Romania.
Stefănescu
M
M
Onu
A
A
Mureşan
D
D
Szegli
G
G
D001327:Autoimmune Diseases / Q000097:blood*; D001345:Autoradiography; D002469:Cell Separation; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008180:Lupus Erythematosus, Systemic / Q000097:blood*; D010748:Phosphopeptides / Q000097:blood / Q000302:isolation & purification; D010766:Phosphorylation / Q000187:drug effects; D019000:Phosphotyrosine; D011233:Precipitin Tests; D015398:Signal Transduction / Q000187:drug effects*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000097:blood / Q000187:drug effects* / Q000302:isolation & purification
D037102:Lectins; D010748:Phosphopeptides; D019000:Phosphotyrosine; D014443:Tyrosine