The myotonin-protein kinase phosphorylates tyrosine residues in normal human skeletal muscle.
As a first approach to study the cellular events involved in myotonic dystrophy, we have produced a polyclonal antibody against a peptide sequence of the predicted gene product. This antibody specifically recognizes a 54 kDa protein in human skeletal muscle. This protein phosphorylates a co-polymer Glu/Tyr but not Myelin Basic Protein. This indicates that the myotonin-protein kinase has a tyrosine kinase activity in human skeletal muscle. This is the first demonstration of the kinase activity of the myotonin-protein kinase.
Etongué-Mayer
P
P
Department of Medicine and Molecular Genetics, CHU Laval Research Center, Ste-Foy, Quebec, Canada.
Faure
R
R
Bouchard
J P
JP
Thibault
M C
MC
Puymirat
J
J
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D006801:Humans; D007158:Immunologic Techniques; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000378:metabolism; D009223:Myotonic Dystrophy / Q000201:enzymology; D065636:Myotonin-Protein Kinase; D010455:Peptides / Q000737:chemistry; D019000:Phosphotyrosine; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
C585517:DMPK protein, human; D009124:Muscle Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; D019000:Phosphotyrosine; D014443:Tyrosine; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases