Fermentation of radiolabeled carbohydrates by a reconstructed continuous-flow culture effective against Salmonella in broiler chicks.
An 81-d-old continuous-flow (CF) culture of broiler cecal bacteria was maintained in a lactose-based broth. The culture had been previously proven effective against Salmonella colonization in young chicks, especially when the chicks were provided dietary lactose. Portions of the CF culture were batch-cultured in glucose- and lactose-based broths containing 14C-labeled lactose, glucose, galactose, or lactic acid to determine the effect of media carbohydrate on fermentation products. Acetic and propionic acids were the major 14C-labeled fermentation products. 14C-Carbohydrates were fermented to lactic acid and then to acetic and propionic acids. Distribution of radiolabeled fermentation products was effected by the broth carbohydrate and the time postinoculation.
Hume
M E
ME
USDA, Food Animal Protection Research Laboratory, College Station, Texas 77845, USA.
Nisbet
D J
DJ
Scanlan
C M
CM
Corrier
D E
DE
Deloach
J R
JR
D000085:Acetates / Q000378:metabolism; D019342:Acetic Acid; D000818:Animals; D000898:Antibiosis; D050260:Carbohydrate Metabolism; D002432:Cecum / Q000382:microbiology*; D002645:Chickens / Q000382:microbiology*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D011422:Propionates / Q000378:metabolism; D012475:Salmonella / Q000254:growth & development*
D000085:Acetates; D003470:Culture Media; D011422:Propionates; C029658:propionic acid; D019342:Acetic Acid