Soluble E-selectin is increased in inflammatory synovial fluid.
OBJECTIVE
To investigate the hypothesis that soluble E-selectin (sE-selectin) may be detected in synovial fluid (SF) and play a role in inflammatory arthritis.


METHODS
We used a sandwich ELISA to measure sE-selectin in the SF of 58 patients with rheumatoid arthritis (RA), 9 with psoriatic arthritis (PsA), 30 with osteoarthritis (OA), 13 with gout, and 9 with calcium pyrophosphate dihydrate crystal deposition disease (CPPD).


RESULTS
SF sE-selectin values in RA (mean 1.49 ng/ml, 0.18-3.90) and PsA (mean 1.36 ng/ml, 0.88-2.31) were significantly higher than those with OA (mean 0.83 ng/ml, 0.00-1.83), gout (mean 1.04 ng/ml, 0.11-3.42), or CPPD (mean 0.80 ng/ml, 0.20-1.47). Elevated SF sE-selectin was associated with elevated serum sE-selectin, erythrocyte sedimentation rate, and SF white blood cell count.


CONCLUSIONS
Our findings suggest that endothelial cell activation and E-selectin may contribute to the development of inflammatory processes.
Carson
C W
CW
Department of Medicine, Mayo Clinic/Foundation, Rochester, MN.
Beall
L D
LD
Hunder
G G
GG
Johnson
C M
CM
Newman
W
W
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001168:Arthritis / Q000209:etiology / Q000378:metabolism*; D015210:Arthritis, Gouty / Q000378:metabolism; D015535:Arthritis, Psoriatic / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000097:blood / Q000378:metabolism*; D002805:Chondrocalcinosis / Q000378:metabolism; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010003:Osteoarthritis / Q000378:metabolism; D012995:Solubility; D013582:Synovial Fluid / Q000378:metabolism*
D015815:Cell Adhesion Molecules; D019040:E-Selectin