title: Differential host-dependent expression of alpha-galactosyl epitopes on viral glycoproteins: a study of eastern equine encephalitis virus as a model.
abstract: The carbohydrate epitope Gal alpha 1-3Gal beta 1-4GlcNAc-R (alpha-galactosyl) is abundantly expressed on cells of non-primate mammals, prosimians and New World monkeys, where it is synthesized by the enzyme alpha 1,3-galactosyltransferase (alpha 1,3GT). Old World monkeys, apes and humans lack alpha 1,3GT and hence do not synthesize alpha-galactosyl epitopes. Instead, these species produce a natural antibody, anti-Gal, which interacts specifically with alpha-galactosyl epitopes and which constitutes up to 1% of circulating immunoglobulins in humans. We have used eastern equine encephalitis (EEE) virus as a model to examine the differential expression of alpha-galactosyl epitopes on the glycoproteins of virus propagated in cells that either produce or lack alpha 1,3GT. As predicted, virus propagated in Vero cells (derived from the African green monkey, an Old World monkey) did not express alpha-galactosyl epitopes. In contrast, virus propagated in mouse 3T3 cells (EEE3T3) expressed approximately 80 alpha-galactosyl epitopes per virion on both the E1 and the E2 envelope glycoproteins. Thus, expression of the alpha-galactosyl epitope on virions paralleled that on host cells. The binding of anti-Gal antibody to these epitopes on EEE3T3 virions partially neutralized virus infectivity, raising the possibility that anti-Gal production in hosts may influence the initial infectious stage of viruses expressing alpha-galactosyl epitopes.
authors.affiliation: Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia 19129.
authors.forename: P M
authors.initials: PM
authors.lastname: Repik
authors.forename: J M
authors.initials: JM
authors.lastname: Strizki
authors.forename: U
authors.initials: U
authors.lastname: Galili
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology*; D002240:Carbohydrate Sequence; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004663:Encephalitis Virus, Eastern Equine / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D005697:Galactosides / Q000276:immunology*; D005700:Galactosyltransferases / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000276:immunology; D011863:Radioimmunoassay; D013045:Species Specificity; D015678:Viral Structural Proteins / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D000939:Epitopes; D005697:Galactosides; D009844:Oligosaccharides; D015678:Viral Structural Proteins; D005700:Galactosyltransferases; C037959:beta-D-galactosyl-N-acetylglucosaminylglycopeptide alpha-1,3-galactosyltransferase