3-isobutyl-1-methylxanthine inhibits the mutagenic activity of 7,12-dimethylbenz[a]anthracene in epithelial liver cells in culture.
The mutagenic activity of 7,12-dimethylbenz[a]anthracene in epithelial liver cells (CHEL) in culture was unaffected by the enhancement of intracellular cAMP induced to different extents and with different mechanisms by forskolin and 3-isobutyl-1-methylxanthine. However, the latter compound exerted antimutagenic effects (> 60%), which may be tentatively ascribed to inhibition of the inducible monooxygenase isoform(s) responsible for the specific biotransformation of 7,12-dimethylbenz[a]anthracene to highly mutagenic metabolites in CHEL cells.
Bertacca
A
A
Institute of Mutagenesis and Differentiation of CNR, Pisa, Italy.
Cini
M
M
Sbrana
M
M
Turchi
G
G
D015056:1-Methyl-3-isobutylxanthine / Q000378:metabolism* / Q000494:pharmacology; D015127:9,10-Dimethyl-1,2-benzanthracene / Q000378:metabolism*; D000818:Animals; D016587:Antimutagenic Agents / Q000378:metabolism*; D001711:Biotransformation / Q000187:drug effects; D002478:Cells, Cultured / Q000378:metabolism; D005576:Colforsin / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D000242:Cyclic AMP / Q000096:biosynthesis; D004790:Enzyme Induction; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D007527:Isoenzymes / Q000037:antagonists & inhibitors; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D016296:Mutagenesis / Q000187:drug effects*; D010105:Oxygenases / Q000037:antagonists & inhibitors*
D016587:Antimutagenic Agents; D007527:Isoenzymes; D005576:Colforsin; D015127:9,10-Dimethyl-1,2-benzanthracene; D000242:Cyclic AMP; D010105:Oxygenases; D007041:Hypoxanthine Phosphoribosyltransferase; D015056:1-Methyl-3-isobutylxanthine