Mast cell activation induces P-selectin-dependent leukocyte rolling and adhesion in postcapillary venules in vivo.
As studied by intravital microscopy, local challenge with the mast cell secretagogue compound 48/80 was found to increase the leukocyte rolling fraction, decrease rolling velocity and induce firm leukocyte adhesion in postcapillary venules of the rat mesentery. These effects of compound 48/80 were inhibited by a monoclonal anti-P-selectin antibody, but not by combined treatment with H1 and H2 histamine-receptor antagonists. Moreover, the response to compound 48/80 was not mimicked by exogenous histamine or 5-hydroxytryptamine (5-HT). These novel findings indicate that mediator(s) other than histamine and 5-HT evoke P-selectin-dependent leukocyte rolling and thereby promote firm leukocyte adhesion in mast cell-dependent inflammation.
Thorlacius
H
H
Department of Physiology & Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Raud
J
J
Rosengren-Beezley
S
S
Forrest
M J
MJ
Hedqvist
P
P
Lindbom
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D006632:Histamine / Q000494:pharmacology; D007958:Leukocyte Count; D007962:Leukocytes / Q000502:physiology*; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D008642:Mesenteric Veins / Q000166:cytology; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000276:immunology / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000494:pharmacology; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D012701:Serotonin; D003189:p-Methoxy-N-methylphenethylamine; D006632:Histamine