Pathogenesis of ascites tumor growth: angiogenesis, vascular remodeling, and stroma formation in the peritoneal lining.
In the accompanying papers, we demonstrated that two murine ascites tumors (MOT and TA3/St) induced peritoneal lining blood vessels to become hyperpermeable to plasma proteins, leading to extravasation of fibrinogen and its clotting to cross-linked fibrin in peritoneal lining tissues (peritoneal wall, mesentery, and diaphragm). In solid tumors, vascular hyperpermeability and fibrin deposition lead to the generation of vascularized connective tissue. In order to determine whether fibrin had similar consequences in ascites tumors, the vasculature and stroma of peritoneal lining tissues were analyzed at successive intervals after i.p. tumor cell injection. In both MOT and TA3/St ascites tumors, the size and number of peritoneal lining microvessels increased significantly by 5-8 days. Subsequently, peritoneal lining vessels increased in cross-sectional area by as much as 15-fold and peritoneal vascular frequency increased by up to 11-fold. Incorporation of [3H]thymidine by mesenteric blood vessels was negligible in control animals but came to involve 20 and 40% of endothelial cells lining mesenteric vessels in MOT and TA3/St ascites tumor-bearing mice, respectively. After an early dramatic increase in cross-sectional area, peritoneal lining microvessels subsequently underwent a novel form of remodeling to smaller average size as the result of transvascular bridging by endothelial cell cytoplasmic processes. Thus, both of the ascites tumors studied here induced angiogenesis and stroma similar to that elicited when these same tumors were grown in solid form. However, stroma developed more slowly in ascites than in solid tumors and was entirely confined to a compartment (peritoneal lining tissues) that was distinct from that (peritoneal cavity) containing the majority of tumor cells and ascites fluid. These findings are consistent with the hypothesis that vascular hyperpermeability, induced in both solid and ascites tumors by tumor cell-secreted vascular permeability factor, is a common early step in tumor angiogenesis, resulting in fibrinogen extravasation, fibrin deposition, and likely other alterations of the extracellular matrix that together stimulate new vessel and fibroblast ingrowth.
Nagy
J A
JA
Department of Pathology, Beth Israel Hospital, Boston, Massachusetts 02215.
Morgan
E S
ES
Herzberg
K T
KT
Manseau
E J
EJ
Dvorak
A M
AM
Dvorak
H F
HF
D000818:Animals; D001202:Ascitic Fluid / Q000209:etiology* / Q000378:metabolism; D002199:Capillary Permeability; D003964:Diaphragm / Q000098:blood supply; D005260:Female; D015674:Mammary Neoplasms, Animal / Q000473:pathology; D008643:Mesentery / Q000098:blood supply / Q000473:pathology*; D051379:Mice; D008809:Mice, Inbred C3H; D008833:Microcirculation / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000209:etiology*; D010051:Ovarian Neoplasms / Q000473:pathology; D010537:Peritoneum / Q000098:blood supply* / Q000473:pathology