title: Functional studies on MEL-14+ and MEL-14- T cells in peripheral lymphoid tissues.
abstract: Functions of MEL-14+ T cells and MEL-14- T cells in peripheral lymphoid tissues were analyzed and compared. The MEL-14- T cells, representing a minor subpopulation of spleen and lymph node T cells, generated considerably higher mixed lymphocyte reaction and mitogen responses than the MEL-14+ T cells in any lymphoid tissues studied. Furthermore, upon stimulation with ConA the MEL-14- CD8+ T cells produced significantly larger amounts of IL-2 and IFN-gamma than MEL-14+ CD8+ T cells did. A similar but less marked observation was obtained with the CD4+ T cell population. Furthermore, when B10.BR mice were immunized with AKR (Mls-1a) spleen cells, the proportion of the Mls-1a reactive V beta 6+ T cells from draining lymph nodes increased and a substantial proportion of the increasing V beta 6+ T cells was shown to be MEL-14-. The present findings on the whole indicate that MEL-14- T cells in the peripheral lymphoid tissues are at functionally high levels and may represent memory cells which have been previously stimulated in vivo.
authors.affiliation: Section of Pathology, Hokkaido University, Sapporo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Ohgama
authors.forename: M
authors.initials: M
authors.lastname: Katoh
authors.forename: M
authors.initials: M
authors.lastname: Hirano
authors.forename: H
authors.initials: H
authors.lastname: Arase
authors.forename: N
authors.initials: N
authors.lastname: Arase-Fukushi
authors.forename: M
authors.initials: M
authors.lastname: Mishima
authors.forename: K
authors.initials: K
authors.lastname: Iwabuchi
authors.forename: K
authors.initials: K
authors.lastname: Ogasawara
authors.forename: K
authors.initials: K
authors.lastname: Onoé
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D019041:L-Selectin; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology*; D008285:Major Histocompatibility Complex / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D016201:Receptors, Lymphocyte Homing / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D017252:CD3 Complex; D015815:Cell Adhesion Molecules; D007376:Interleukin-2; D016201:Receptors, Lymphocyte Homing; D019041:L-Selectin; D007371:Interferon-gamma