title: A new mock circulatory loop and its application to the study of chemical additive and aortic pressure effects on hemolysis in the Penn State electric ventricular assist device.
abstract: A new mock circulatory loop was developed for hemolysis studies associated with the Penn State electric ventricular assist device (EVAD). This flow loop has several advantages over previously designed loops. It is small enough to accommodate experiments in which only single units of blood are available, it is made out of biocompatible materials, it incorporates good geometry, and it provides normal physiological pressures and flows to both the aortic outlet and the venous inlet of the pumping device. Experiments with reduced aortic pressure but normal cardiac output showed that hemolysis in a loop with normal aortic blood pressure was significantly higher than that in a loop with lowered aortic pressure, thereby illustrating the importance of maintaining loop pressures as close as possible to those found in vivo. This data also imply that blood traveling through the left ventricle in an artificial heart may be subject to higher hemolysis rates than that traversing the right ventricle. Another set of experiments to determine the effects of 4 hemolysis or drag-reducing agents (Pluronic F-68, Dextran-40, Polyox WSR-301, and Praestol 2273TR) on blood trauma due to the EVAD and associated valves was performed. Results indicated that none of the additives significantly reduced hemolysis under the conditions found in the mock loop. Finally, a compilation of data gathered in these experiments showed that the index of hemolysis (IH) is dependent on hematocrit (HCT), which suggests that another parameter, IH/HCT, may be more suited to the quantification of hemolysis.
authors.affiliation: Bioengineering and Chemical Engineering Departments, Pennsylvania State University, University Park 16802.
authors.forename: L A
authors.initials: LA
authors.lastname: Garrison
authors.forename: J A
authors.initials: JA
authors.lastname: Frangos
authors.forename: D B
authors.initials: DB
authors.lastname: Geselowitz
authors.forename: T C
authors.initials: TC
authors.lastname: Lamson
authors.forename: J M
authors.initials: JM
authors.lastname: Tarbell
mesh_terms: D000180:Acrylic Resins; D001011:Aorta / Q000502:physiology*; D001794:Blood Pressure; D003911:Dextrans; D006353:Heart-Assist Devices / Q000009:adverse effects; D006400:Hematocrit; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008961:Models, Structural; D011060:Poloxalene; D011092:Polyethylene Glycols
chemical_list: D000180:Acrylic Resins; D003911:Dextrans; C054303:separan AP 273; D011092:Polyethylene Glycols; D011060:Poloxalene