Monoclonal antibodies against Schistosoma mekongi surface tegumental antigens.
Monoclonal antibodies were produced from naturally infected BALB/c mice. Thirteen hybridomas which were found to produce monoclonal antibodies against surface tegumental antigens of Schistosoma mekongi by ELISA assay were used in this study. The antigen specificities of hybridomas reactive with surface tegumental antigens were characterized and localized by immunoblotting analysis and Avidin-Biotin method. Of the 13 hybridomas, only three produced monoclonal antibodies to the single epitopes in the surface tegumental antigens. These epitopes (125 kDa, 97 kDa and 38 kDa) have been found to be the major antigenic components of the surface tegument of S. mekongi. The 38 kDa antigen was found to associate with the surface tegumental layers, the muscular layers lying just beneath the tegument, as well as in the gut surface. The 97 and 125 kDa antigens were detectable only in the surface tegumental area. The biochemical identity of these proteins or glycoproteins is unknown. However, these antigens have also been described in S. japonicum and S. mansoni.
Viyanant
V
V
Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Gajanadana
O
O
Upatham
E S
ES
Sobhon
P
P
Kruatrachue
M
M
Ahmed
S
S
Ardseungnoen
P
P
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D001360:Avidin; D001710:Biotin; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006825:Hybridomas; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D012547:Schistosoma / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000939:Epitopes; D001360:Avidin; D001710:Biotin