Substance P-like immunoreactivity in the hypothalamic suprachiasmatic nucleus of Phodopus sungorus--relation to daytime, photoperiod, sex and age.
The immunohistochemical distribution of substance P (SP) in the hypothalamic suprachiasmatic nucleus (SCN) was studied in adult male and female Djungarian hamsters (Phodopus sungorus) held under either long or short photoperiods. Intact animals were killed by perfusion with a fixative at the middle of the light or dark periods, respectively. The tissue was processed by routine immunohistochemical methods. Perikarya exhibiting SP-like immunoreactivity (LI) were found in the SCN of animals of all groups. These cell bodies predominantly were restricted to a distinct portion of the nucleus extending less than 150 microns rostrocaudally and were often concentrated in its lateral aspect. SP-LI fibers were rarely observed in the SCN, however, other hypothalamic parts, e.g. anterior and paraventricular regions, exhibit strong SP-LI innervation patterns. Sex-related differences were not observed. Long-term exposure to short days decreased the number of neurons exhibiting SP-LI by approximately 60% when compared to long-day animals at both day- and nighttime. At night, SP-LI neurons were augmented in number by 34% (long-day group) and 56% (short-day group). Further, the numbers of SP-LI perikarya in the SCN of aged hamsters at day- and nighttime were augmented 3- to 4-fold when compared to adult animals. These results suggest that substance P in the SCN is involved in the regulation of circadian and seasonal mechanisms in this highly photoperiodic rodent species.
Reuss
S
S
Department of Anatomy, Johannes Gutenberg-University, Mainz, Germany.
Bürger
K
K
D000375:Aging / Q000502:physiology*; D000818:Animals; D002940:Circadian Rhythm; D006224:Cricetinae; D003624:Darkness; D005260:Female; D007150:Immunohistochemistry; D008027:Light; D008297:Male; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D017111:Phodopus; D012737:Sex Factors; D013373:Substance P / Q000032:analysis / Q000378:metabolism*; D013493:Suprachiasmatic Nucleus / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D013997:Time Factors
D013373:Substance P