[The isolation of an antigen, species-specific in immunochemical tests, from the culture broth of yeastlike fungi in the genus Candida].
In this work an antigen obtained from the culture fluid of C. tropicalis strain 928 is described. The strain was cultivated in a synthetic culture medium in fermenters. The culture fluid was concentrated in cartridges and then on membranes. The concentrate was electrophoretically separated in an agar block. The isolated fraction was purified on Con-A Sepharose. The antigen thus obtained proved to be homogeneous in immunophoresis and in immunodiffusion with homologous serum to the culture fluid. In immunodiffusion and immunophoresis this purified fraction did not precipitate with sera to antigens obtained from the culture fluids of strains of other Candida species.
Dolgikh
M S
MS
Kravtsov
E G
EG
D000818:Animals; D000946:Antigens, Fungal / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D002175:Candida / Q000276:immunology*; D003470:Culture Media; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D007114:Immunization; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D011817:Rabbits; D013045:Species Specificity; D013997:Time Factors
D000946:Antigens, Fungal; D003470:Culture Media; D000939:Epitopes