Production of hematopoietic stem cell-chemotactic factor by bone marrow stromal cells.
Chemotactic factors produced by stromal cells in the bone marrow are characterized. Two kinds of factors produced by stromal cell lines are identified using blind-well Boyden's Chambers; one is a neutrophil-chemotactic factor and the other a hematopoietic stem cell (HSC)-chemotactic factor. The latter attracts blastic cells in a low-density fraction, which are Thy1lo, wheat germ agglutinin (WGA)hi, H-2Khi, Ly-1-, Ly-2-, L3T4-, Ly5-, and slg-. The molecular weight of this HSC-chemotactic factor is estimated to be more than 200 kD. Putative cytokines and growth factors, such as granulocyte colony-stimulating factor (CSF), macrophage CSF, granulocyte-macrophage CSF, stem cell factor (SCF), interleukin (IL)-6, and IL-3, do not possess HSC-chemotactic activity. These findings strongly suggest that bone marrow stromal cells produce a new factor that attracts HSCs.
Cherry
Department of Pathology, Kansai Medical University, Osaka, Japan.
Yasumizu
R
R
Toki
J
J
Asou
H
H
Nishino
T
T
Komatsu
Y
Y
Ikehara
S
S
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis / Q000096:biosynthesis; D001853:Bone Marrow / Q000378:metabolism*; D001854:Bone Marrow Cells; D002460:Cell Line; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000032:analysis / Q000096:biosynthesis*; D002633:Chemotaxis / Q000187:drug effects; D003114:Colony-Forming Units Assay; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000494:pharmacology*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology; D015850:Interleukin-6 / Q000494:pharmacology; D007700:Kinetics; D016173:Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008809:Mice, Inbred C3H; D011994:Recombinant Proteins / Q000494:pharmacology; D019089:Stem Cell Factor; D018800:Thy-1 Antigens
D000954:Antigens, Surface; D002630:Chemotactic Factors; D017077:Culture Media, Conditioned; D016207:Cytokines; D006133:Growth Substances; D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D015850:Interleukin-6; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D018800:Thy-1 Antigens; C086022:hematopoietic stem cell-chemotactic factor; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor