Splenic lymphoma with villous lymphocytes: diagnosis by flow cytometry and immunocytochemistry.
Splenic lymphoma with villous lymphocytes (SLVL) is a recently described low-grade non-Hodgkin's lymphoma that may be confused with chronic lymphocytic leukemia and hairy cell leukemia. Herein we report a case of SLVL and show how clinical features, cellular morphology, and flow cytometric analysis are used to distinguish among these three disorders. A newly developed immunocytochemical technique that allows detection of tartrate-resistant acid phosphatase on formalin-fixed, paraffin-embedded specimens was useful in refining our diagnosis of SLVL. Establishing the precise diagnosis is important because treatment is different for each of the three chronic lymphoproliferative malignancies.
Brune
M B
MB
Department of Internal Medicine, University of Louisville, KY, USA.
Cain
D L
DL
Cardwell
E M
EM
Janckila
A J
AJ
D000368:Aged; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000473:pathology; D008297:Male