Galanin immunoreactivity in autonomic innervation of the cat eye.
In an immunohistochemical study, we find that galanin is much more widely distributed in the peripheral innervation of the cat eye than in other animals so far examined. Previous studies of rat and pig eyes have revealed sparse galanin-positive nerves that presumably originate in the trigeminal ganglion. In contrast, the cat has a rich supply of galanin-containing nerve fibers throughout the uvea. Galanin-positive varicose nerves concentrate densely in iris muscles and distribute more sparsely in the ciliary muscle. The ciliary processes have a plexus of galanin-positive nerves underlying the ciliary epithelium at their base and positive nerve fibers coursing within their stroma. The ciliary artery and its branch vessels in the uvea are invested with a dense plexus of galanin-positive nerves. All autonomic ganglia supplying the eye contain cells that express galanin. It is present in 97% of superior cervical ganglion cells, coexisting with both tyrosine hydroxylase and neuropeptide Y; in 80% of pterygopalatine ganglion cells, most of which also contain vasoactive intestinal peptide; and in approximately 25% of ciliary ganglion cells. After unilateral superior cervical ganglionectomy, galanin-positive nerves almost totally disappear from the iris muscles, demonstrating that they are predominantly of sympathetic origin. Galanin-positive nerves investing the ciliary artery and choroidal blood vessels are not detectably reduced by sympathectomy, indicating that perivascular parasympathetic nerves from the pterygopalatine ganglion also express galanin. Other galanin-containing nerves in the eye can originate from the trigeminal and ciliary ganglia. The prominence of galanin in the ocular autonomic innervation of the cat provides an opportunity to explore the physiological effects of this neuropeptide in the eye.
Grimes
P A
PA
Department of Ophthalmology, University of Pennsylvania, Philadelphia 19104.
McGlinn
A M
AM
Koeberlein
B
B
Stone
R A
RA
D000818:Animals; D001341:Autonomic Nervous System / Q000166:cytology / Q000378:metabolism*; D002415:Cats / Q000502:physiology*; D003714:Denervation; D005123:Eye / Q000294:innervation*; D005260:Female; D019004:Galanin; D005725:Ganglia, Autonomic / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D009479:Neuropeptides / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D012016:Reference Values
D009479:Neuropeptides; D010455:Peptides; D019004:Galanin