Retrovirus tests of human leukemia/lymphoma cell lines at DSM.
Permanently established human cell lines can produce several retroviruses. It is important to routinely test such cell lines for human T cell lymphotropic virus (HTLV) type I and II, and for human immunodeficiency virus (HIV) type 1 and 2 in order to exclude any potential biohazard from cell lines producing human retroviruses. Reverse transcriptase assay, polymerase chain reaction, and dot-blot hybridization of in-vitro amplified DNA with virus-specific probes are used.
Häne
B G
BG
German Collection of Microorganisms and Cell Cultures (DSM), Braunschweig, Germany.
Drexler
H G
HG
D003264:Containment of Biohazards; D004279:DNA, Viral / Q000032:analysis; D017977:Deltaretrovirus / Q000302:isolation & purification*; D006678:HIV / Q000302:isolation & purification*; D006801:Humans; D007938:Leukemia / Q000382:microbiology*; D008223:Lymphoma / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014407:Tumor Cells, Cultured
D004279:DNA, Viral; D012194:RNA-Directed DNA Polymerase