Cathepsin B inhibition in two models of acute pancreatitis.
The possible role of cathepsin B in the pathogenesis of two forms of acute pancreatitis was studied using the cathepsin B inhibitor known as E-64. In an edematous, nonfatal pancreatitis induced by supramaximal doses of cerulein, increases in the serum amylase and lipase levels were less pronounced in rats pretreated with E-64. Other parameters of pancreatic injury were unaffected by inhibition of cathepsin B. In a necrohemorrhagic type of pancreatic injury induced by retrograde infusion of bile salts into the pancreatic duct, E-64 partially attenuated increases in serum levels of amylase and lipase, and in addition, reduced the activation of trypsinogen. However, the high mortality in this model of pancreatitis was not modified.
Korsten
M A
MA
Alcohol Research Center, Bronx VA Medical Center, NY 10468.
Długosz
J W
JW
D000208:Acute Disease; D000681:Amylases / Q000097:blood; D000818:Animals; D002401:Cathepsin B / Q000037:antagonists & inhibitors / Q000502:physiology*; D002108:Ceruletide; D004195:Disease Models, Animal; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D008049:Lipase / Q000097:blood; D008297:Male; D010195:Pancreatitis / Q000201:enzymology / Q000209:etiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D002108:Ceruletide; D008049:Lipase; D000681:Amylases; D002401:Cathepsin B; D007930:Leucine; C024974:E 64