title: Binding and specificity of major immunoglobulin classes of preformed human anti-pig heart antibodies.
abstract: Preformed human anti-pig antibodies isolated from perfused pig hearts were used to analyze the binding of various immunoglobulin classes to cultured pig kidney cells. All anti-pig immunoglobulins (i.e., IgG, IgA, and IgM) were localized on the cell surface by the use of an indirect immunofluorescence technique. Anti-pig immunoglobulins also competed for the pig cell surface epitopes with Griffonia simplicifolia lectin (GS-I-B4), which is specific for alpha-galactosyl residues. This study provides further evidence that preformed human antibodies recognizing alpha-galactosyl-containing epitopes (anti-gal antibodies) could be an important factor in hyperacute rejection of pig organs.
authors.affiliation: Oklahoma Medical Research Foundation, Oklahoma City 73104.
authors.forename: E
authors.initials: E
authors.lastname: Koren
authors.forename: F A
authors.initials: FA
authors.lastname: Neethling
authors.forename: S
authors.initials: S
authors.lastname: Richards
authors.forename: M
authors.initials: M
authors.lastname: Koscec
authors.forename: Y
authors.initials: Y
authors.lastname: Ye
authors.forename: N
authors.initials: N
authors.lastname: Zuhdi
authors.forename: D K
authors.initials: DK
authors.lastname: Cooper
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001667:Binding, Competitive; D002478:Cells, Cultured; D000939:Epitopes; D005690:Galactose; D006801:Humans; D007136:Immunoglobulins / Q000145:classification / Q000378:metabolism*; D007668:Kidney / Q000166:cytology / Q000378:metabolism*; D037102:Lectins; D009206:Myocardium / Q000276:immunology*; D013552:Swine
chemical_list: D000939:Epitopes; D007136:Immunoglobulins; D037102:Lectins; D005690:Galactose