Lack of chromosomal aberration and micronucleus induction in human lymphocytes exposed to pulsed magnetic fields.
We exposed human peripheral blood lymphocyte cultures to 50 Hz pulsed magnetic fields (PMFs) in order to evaluate a possible genotoxic effect of such non-ionizing radiation. The genotoxic effect was evaluated in terms of both micronucleus (MN) induction and classical chromosomal aberrations (CA); the mitotic index (MI) was also calculated. Khalil and Qassem (1991) found chromosomal and chromatid breaks and mitotic delay in human lymphocytes exposed for 24, 48 and 72 h to a field with characteristics similar to those used in our laboratory. These data are in contrast with our results previously reported in terms of MN induction using the cytokinesis block method (Scarfi et al., 1991). In this study lymphocytes from five healthy human donors were examined with the above mentioned tests. No genotoxic effects and increased MI were found in exposed samples compared to the control ones, in agreement with our previous results.
Scarfì
M R
MR
CNR-IRECE, Naples, Italy.
Lioi
M B
MB
Zeni
O
O
Franceschetti
G
G
Franceschi
C
C
Bersani
F
F
D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D008280:Magnetics; D015162:Micronucleus Tests; D008940:Mitotic Index