Restoring and immunohistological examination of Feulgen stained avian embryonic tissues using iron hematoxylin and endothelial cell specific antibody.
The immunohistological method described here permits re-examination of previously Feulgen stained quail-chick chimera tissues for vascular development using the monoclonal antibody QH1 which specifically recognizes quail hemangioblastic cells. Weigert's iron hematoxylin has been used to restore faded or bleached Feulgen stained chimeric avian tissues. Species-specific differences in nuclear morphology are as evident with iron hematoxylin staining as they are with Feulgen staining.
Himmelmann
S
S
Department of Anatomy and Embryology, Ruhr-University Bochum, Germany.
Hake
H
H
Jacob
H J
HJ
D000818:Animals; D000918:Antibody Specificity; D002467:Cell Nucleus / Q000648:ultrastructure; D002642:Chick Embryo; D002678:Chimera; D004396:Coloring Agents; D004625:Embryo, Nonmammalian / Q000166:cytology*; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D006416:Hematoxylin; D007150:Immunohistochemistry; D011784:Quail / Q000196:embryology*; D012394:Rosaniline Dyes; D013045:Species Specificity; D013194:Staining and Labeling
D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes; D006416:Hematoxylin