Carboxymethyldextran lactone: a preactivated polymer for amine conjugations.
The linking of amino haptens to carboxymethyldextran (CMD) requires carboxyl activation, for example, via carbodiimdes. We have discovered that substantial N-acylurea, derived from these carbodiimides, can be trapped on the CMD backbone. As an alternative, CMD can be conveniently lactonized by heating in inert solvents, and this carboxymethyldextran lactone (CLD) can be employed directly for amine conjugation.
Heindel
N D
ND
Department of Chemistry, Lehigh University, Bethlehem, Pennsylvania 18105.
Kauffman
M A
MA
Akyea
E K
EK
Engel
S A
SA
Frey
M F
MF
Lacey
C J
CJ
Egolf
R A
RA
D000588:Amines / Q000737:chemistry*; D003911:Dextrans / Q000138:chemical synthesis*; D004337:Drug Carriers / Q000138:chemical synthesis; D007783:Lactones / Q000138:chemical synthesis; D011108:Polymers / Q000138:chemical synthesis
D000588:Amines; D003911:Dextrans; D004337:Drug Carriers; D007783:Lactones; D011108:Polymers; C014392:carboxymethyl dextran