pH and temperature modulate norepinephrine-dependent changes in endothelial permeability.
To evaluate the role of pH and temperature in norepinephrine (NE)-mediated decreases in endothelial permeability, we studied bovine pulmonary arterial endothelial monolayers at pH values of 4-9 and at temperatures of 35-39 degrees C. Only extremes of pH-modulated endothelial permeability and temperature had no effect. Although both NE and 3-isobutyl-1-methylxanthine decreased endothelial permeability, their effects were diminished by low pH and high temperature. Fever and acidosis may contribute to the edema seen in septic shock by a novel mechanism: attenuation of the barrier-improving function of NE.
Griffin
M P
MP
Department of Pediatrics, University of Virginia, Charlottesville 22901.
Moorman
J R
JR
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000418:Albumins / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D009638:Norepinephrine / Q000502:physiology*; D010539:Permeability; D011651:Pulmonary Artery / Q000166:cytology / Q000502:physiology; D013696:Temperature
D000418:Albumins; D015056:1-Methyl-3-isobutylxanthine; D009638:Norepinephrine