Immunofluorescent localization of platelet-activating factor (PAF) in the rat.
An immunofluorescent staining method for detecting platelet-activating factor (PAF) is described. This method employs a polyclonal anti-PAF rabbit antibody. When rat brain, heart, lung, liver or kidney tissue was stained using this method, the heart, lung and kidney exhibited PAF-specific staining. Analysis of the amount of PAF in different organs, either by immunofluorescence or by bioassay, showed that kidney tissue contains the greatest amount of PAF.
Hirashima
Y
Y
Department of Neurosurgery, Toyama Medical and Pharmaceutical University, Japan.
Kato
R
R
Endo
S
S
Takaku
A
A
Karasawa
K
K
Nojima
S
S
D000818:Animals; D001923:Brain Chemistry; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes / Q000737:chemistry; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry; D008168:Lung / Q000737:chemistry*; D008297:Male; D008856:Microscopy, Fluorescence; D009206:Myocardium / Q000737:chemistry*; D010972:Platelet Activating Factor / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D010972:Platelet Activating Factor