Nitric oxide modulates prostacyclin biosynthesis in the lung of endotoxin-treated rats.
In this study we have investigated the correlation between prostaglandin generation and the L-arginine:nitric oxide (NO) pathway in the lung of rats challenged with lipopolysaccharide. We found that in rats treated with L-arginine, the amount of prostacyclin, measured as 6-keto-PGF1 alpha, was significantly increased. Conversely in rats treated with NG-nitro-L-arginine methyl ester the production of 6-keto-PGF1 alpha by lung was significantly decreased. Chopped lungs that had been removed from rats challenged with lipopolysaccharide and were incubated overnight with L-arginine or nitric oxide inhibitors, NG-nitro-L-arginine methyl ester or NG-monomethyl-L-arginine, released respectively increased or decreased amount of 6-keto-PGF1 alpha. These results suggest that, in the rat, lung endogenous nitric oxide may modulate prostanoid production.
Sautebin
L
L
Department of Experimental Pharmacology, University of Naples Federico II, Italy.
Di Rosa
M
M
D015121:6-Ketoprostaglandin F1 alpha / Q000096:biosynthesis; D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D000818:Animals; D001120:Arginine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004790:Enzyme Induction; D011464:Epoprostenol / Q000096:biosynthesis*; D066298:In Vitro Techniques; D007279:Injections, Subcutaneous; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D008168:Lung / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019001:Nitric Oxide Synthase; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D019323:omega-N-Methylarginine
D008070:Lipopolysaccharides; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D015121:6-Ketoprostaglandin F1 alpha; D001120:Arginine; D011464:Epoprostenol; D019001:Nitric Oxide Synthase; D011451:Prostaglandin-Endoperoxide Synthases; D000594:Amino Acid Oxidoreductases; D019331:NG-Nitroarginine Methyl Ester