Serological responses to different genotypes of hepatitis C virus in France.
The relationship between hepatitis C virus (HCV) genotypes and antibody status was studied in 104 chronic non-A, non-B hepatitis patients and asymptomatic HCV-infected blood donors. On the basis of amplification of the nonstructural protein 3 (NS3) coding region by PCR and hybridization with specific probes, 55 and 42 patients were identified as being infected with type I and type II, respectively, according to the classification by H. Okamoto, K. Kurai, S. Okada, K. Yamamoto, H. Lizuka, T. Tanaka, S. Fukuda, F. Tsudaand, and S. Mishiro (Virology 188:331-341, 1992). All samples were tested for antibodies to 5.1.1, C-100, C-33, and C-22 proteins by a second-generation recombinant immunoblot assay. Among 97 patients with known HCV genotypes, 31 of 42 patients infected with type II and 24 of 55 infected with type I had antibodies against all four antigens (P < 0.01). In the type II-infected group, more patients had detectable antibodies to 5.11, C-33, and C-22 proteins than in the type I group (P < 0.05). No difference was found in the serological response to C-100 between the two groups.
Alonso
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Unité de Recherche sur les Hepatites, le SIDA et les Retrovirus Humains, Institut National de la Santé et de la Recherche Médicale, Lyon, France.
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Lamelin
J P
JP
de Sanjosé
S
S
Vitvitski
L
L
Li
J
J
Berby
F
F
Lambert
V
V
Cortey
M L
ML
Trépo
C
C
D001483:Base Sequence; D001782:Blood Donors; D002353:Carrier State / Q000276:immunology; D002908:Chronic Disease; D005602:France / Q000453:epidemiology; D005838:Genotype; D016174:Hepacivirus / Q000235:genetics*; D006508:Hepatitis Antibodies / Q000096:biosynthesis / Q000097:blood*; D006526:Hepatitis C / Q000453:epidemiology / Q000235:genetics / Q000276:immunology*; D018937:Hepatitis C Antibodies; D006801:Humans; D015151:Immunoblotting; D008969:Molecular Sequence Data; D017361:Viral Nonstructural Proteins / Q000235:genetics; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases