title: Isolation of rfb gene clusters directing the synthesis of O polysaccharides consisting of mannose homopolymers and serological analysis of lipopolysaccharides.
abstract: Four serotypes of two genera, Escherichia coli O8 and O9 and Klebsiella O3 and O5, produce the O polysaccharides consisting of mannose homopolymers. Previously, we reported the isolation and expression of E. coli O9 rfb in E. coli K-12 strains (Kido et al, J. Bacteriol., 171: 3629-3633, 1989). In this study, R' plasmids carrying his-rfb region of the other three strains were isolated and expressed in E. coli K-12 strain. Serological study of lipopolysaccharides (LPS) synthesized in E. coli K-12 strain was carried out. His-linked rfb genes from E. coli O9 and Klebsiella O3 directed the synthesis of O polysaccharides with the same antigenicity as those of the parental strains in E. coli K-12 strain. On the other hand, rfb genes from E. coli O8 and Klebsiella O5 directed the synthesis of O polysaccharides which were antigenically not identical but partially common to those of the parental strains. A rough strain derived from E. coli O8 synthesized LPS which showed the identical antigenicity as the wild strain when the his-rfb region of E. coli O8 was introduced. The results suggest that some genes located distantly from his are additionally required to complete the synthesis of O polysaccharides of E. coli O8 and Klebsiella O5.
authors.affiliation: Department of Bacteriology, Nagoya University School of Medicine, Aichi, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Saeki
authors.forename: N
authors.initials: N
authors.lastname: Kido
authors.forename: T
authors.initials: T
authors.lastname: Sugiyama
authors.forename: M
authors.initials: M
authors.lastname: Ohta
authors.forename: T
authors.initials: T
authors.lastname: Iwashita
authors.forename: K
authors.initials: K
authors.lastname: Uchiya
authors.forename: N
authors.initials: N
authors.lastname: Kato
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000276:immunology; D005798:Genes, Bacterial; D007709:Klebsiella / Q000145:classification / Q000235:genetics / Q000276:immunology; D008070:Lipopolysaccharides / Q000097:blood* / Q000737:chemistry; D008358:Mannose; D008969:Molecular Sequence Data; D005810:Multigene Family; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000235:genetics*
chemical_list: D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D008358:Mannose