Potentiation of GM-CSF or G-CSF induced mobilization of circulating progenitor cells by pretreatment with IL-3 and harvest by apheresis.
Recombinant human colony-stimulating factors (CSFs) are increasingly used for mobilizing progenitor cells in the peripheral blood (PB). 7 patients were treated with GM-CSF or G-CSF at a dose of 5 micrograms/kg/d for 5 days and after a treatment free interval received another cycle of the same cytokine after pretreatment with IL-3. Pretreatment with 5 micrograms/kg/d of IL-3 for 7 days did not mobilize by itself but significantly potentiated GM-CSF or G-CSF induced mobilization of PB progenitor cells in all 5 patients tested. As compared to the mobilization capacity of GM-CSF or G-CSF alone, 7 day IL-3 pretreatment potentiated maximum values of CFU-GM by 3.7-6.3 fold in patients receiving GM-CSF (n = 2) and by 1.8-3.3 fold in patients receiving G-CSF leading to a 10-23 fold increase in numbers of circulating CFU-GM by the combination IL-3/GM-CSF, and a 26-101 fold increase by IL-3/G-CSF, respectively. Stem cell apheresis in patients treated with IL-3 + G-CSF or GM-CSF yielded enough PB-stem cells to ensure complete engraftment after ablative chemotherapy.
Höcker
P
P
Department of Transfusionmedicine, University Hospital, Vienna, Austria.
Geissler
K
K
Kurz
M
M
Wagner
A
A
Gerhartl
K
K
D001781:Blood Component Removal; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007377:Interleukin-3 / Q000008:administration & dosage* / Q000009:adverse effects; D009369:Neoplasms / Q000628:therapy*; D014182:Transplantation, Autologous
D007377:Interleukin-3; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor