Reversal of selenium and zinc deficiencies in chronic hemodialysis patients by intravenous sodium selenite and zinc gluconate supplementation. Time-course of glutathione peroxidase repletion and lipid peroxidation decrease.
In six chronic dialyzed uremic patients, an intravenous sodium selenite (Se 50 micrograms during 5 wk and then 100 micrograms) and zinc gluconate (Zn 5 mg) supplementation was performed during 20 wk at each dialysis session three times weekly. Before supplementation, plasma Se and Zn, plasma and erythrocytes (RBC) antioxidant metallo-enzymes glutathione peroxidase (GPX), and superoxide dismutase (SOD) were significantly decreased, whereas lipid peroxidation (as thiobarbituric acid reactants TBARs) was increased. To obtain a significative change in plasma selenium, we had to use an Se dose of 100 micrograms/dialysis session. Then, treatment-increased plasma Se (from 0.58 +/- 0.09 to 0.89 +/- 0.16 mumol/L) led to a repletion of RBC-GPX (from 29.6 +/- 6 to 43 +/- 5.8 U/g Hb) and increased plasma GPX levels (from 62 +/- 13 to 151 +/- 43 U/L). Plasma Zn and RBC-SOD did not vary significantly. The change of TBARs was not observed between wk 1 and 4. They decreased significantly between wk 4 (4.80 +/- 0.21 mumol/L) and wk 20 (4.16 +/- 0.26 mumol/L). We noted a low correlation between TBARs and plasma GPX. A strong correlation was observed between Se and plasma GPX. The reversal of Se deficiencies should reduce oxidative damage observed in these patients.
Richard
M J
MJ
Laboratoire de Biochimie C, A.G.D.U.C. Hopital A. Michallon, Grenoble, France.
Ducros
V
V
Forêt
M
M
Arnaud
J
J
Coudray
C
C
Fusselier
M
M
Favier
A
A
D000368:Aged; D000704:Analysis of Variance; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D005942:Gluconates / Q000008:administration & dosage / Q000494:pharmacology*; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D007275:Injections, Intravenous; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis / Q000009:adverse effects*; D012643:Selenium / Q000097:blood / Q000172:deficiency*; D018038:Sodium Selenite / Q000008:administration & dosage / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000097:blood; D014511:Uremia / Q000378:metabolism; D015032:Zinc / Q000097:blood / Q000172:deficiency*
D005942:Gluconates; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium; D018038:Sodium Selenite; D015032:Zinc; C030691:gluconic acid