Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies.
Monoclonal antibodies (MAbs) to two U.S. isolates of porcine reproductive and respiratory syndrome (PRRS) virus were prepared. Two MAbs specifically recognized a conserved epitope on the putative 15-kDa nucleocapsid protein of U.S. and European isolates of PRRS virus. Four other MAbs recognized epitopes on the 15-kDa protein of U.S. but not European isolates of PRRS virus. Collectively, this indicates that PRRS viruses contain both conserved and divergent epitopes on the 15-kDa viral protein.
Nelson
E A
EA
Department of Veterinary Science, South Dakota State University, Brookings 57007.
Christopher-Hennings
J
J
Drew
T
T
Wensvoort
G
G
Collins
J E
JE
Benfield
D A
DA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000302:isolation & purification; D018018:Arterivirus / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification*; D000939:Epitopes / Q000302:isolation & purification; D005060:Europe; D005260:Female; D005831:Genital Diseases, Female / Q000382:microbiology / Q000662:veterinary*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014481:United States; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D014764:Viral Proteins