title: Halophilic protein stabilization by the mild solubilizing agents nondetergent sulfobetaines.
abstract: In this work, experiments performed on pig heart and halophilic malate dehydrogenase as well as halophilic elongation factor Tu demonstrate a protein stabilization property from the recently described mild solubilizing agents nondetergent sulfobetaines. A practical application is given by the separation of halophilic bacteria elongation factor Tu and halophilic malate dehydrogenase by high-performance ion-exchange chromatography achieved at reduced salt levels without significant loss of activity.
authors.affiliation: Institut Laue-Langevin, Grenoble, France.
authors.forename: L
authors.initials: L
authors.lastname: Vuillard
authors.forename: D
authors.initials: D
authors.lastname: Madern
authors.forename: B
authors.initials: B
authors.lastname: Franzetti
authors.forename: T
authors.initials: T
authors.lastname: Rabilloud
mesh_terms: D000818:Animals; D001622:Betaine / Q000494:pharmacology*; D004355:Drug Stability; D004795:Enzyme Stability; D008291:Malate Dehydrogenase / Q000737:chemistry*; D010444:Peptide Elongation Factor Tu / Q000737:chemistry*; D013552:Swine
chemical_list: D001622:Betaine; D008291:Malate Dehydrogenase; D010444:Peptide Elongation Factor Tu