title: Direct evidence for SAA deposition in tissues during murine amyloidogenesis.
abstract: To study the mechanism of amyloid deposition, the nature of amyloid proteins formed in experimental murine amyloidosis, was examined. Spleen specimens, 15-60 mg, were homogenized and extracted using aqueous acidic acetonitrile, in a recently developed procedure, making it possible to obtain amyloid proteins from minute amounts of tissue. The extracted material, 1.5-4 mg, was analysed by Western blotting and ELISA using antibodies recognizing differentially proteins AA and SAA. Two immunoreactive proteins of 8 and 12 KDa were isolated and subjected to amino acid analysis and N-terminal sequence determination. The results of immunochemical and chemical examination showed that the 8 and 12 KDa proteins represented proteins AA and SAA, respectively. The data obtained provide new direct evidence for SAA in tissues during murine amyloidogenesis.
authors.affiliation: Heller Institute of Medical Research, Chaim Sheba Medical Center, Tel-Hashomer, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Yakar
authors.forename: B
authors.initials: B
authors.lastname: Kaplan
authors.forename: A
authors.initials: A
authors.lastname: Livneh
authors.forename: B
authors.initials: B
authors.lastname: Martin
authors.forename: K
authors.initials: K
authors.lastname: Miura
authors.forename: Z
authors.initials: Z
authors.lastname: Ali-Khan
authors.forename: S
authors.initials: S
authors.lastname: Shtrasburg
authors.forename: M
authors.initials: M
authors.lastname: Pras
mesh_terms: D000595:Amino Acid Sequence; D000686:Amyloidosis / Q000378:metabolism*; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis; D000685:Serum Amyloid A Protein / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism*
chemical_list: D000685:Serum Amyloid A Protein; D012313:RNA