Quantitative characterization of the CD5 bearing lymphocyte population in the peripheral blood of normal sheep.
Quantitative characterization of the circulating CD5+ lymphocyte population in sheep was performed using monoclonal antibodies against ovine CD5, ovine CD2, and ovine sIgM. A total of fifteen sheep were examined biweekly over a period of 2.5 months. The total CD5+ population was measured using single color flow cytometry, while CD5+ cells co-expressing sIgM or CD2 were determined by two color flow cytometric analysis. The total CD5+ cell population comprised 31.5-65.4% of gated lymphocytes with a mean value of 44.9%. Absolute CD5+ cell numbers varied from 1075 to 8007 cells mm-3, with a mean value of 3149 cells mm-3. The mean proportion of CD5+ B-cells was 4.0%, with a mean absolute value of 270 cells mm-3. The mean proportion of CD5+/CD2+ cells was 12.1% while that of CD5+/CD2- cells was 28.6%, corresponding to mean absolute values of 727 and 1794 cells mm-3 respectively. Significant variation in CD5+ lymphocyte numbers in all individual animals occurred over the time span of the study. The results of this study provide a baseline from which to accurately assess CD5+ cell populations in neoplastic and infectious diseases.
Birkebak
T A
TA
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman 99164-7040.
Palmer
G H
GH
Davis
W C
WC
McElwain
T F
TF
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D018801:CD2 Antigens; D018956:CD5 Antigens; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D007075:Immunoglobulin M / Q000032:analysis; D007958:Leukocyte Count; D008214:Lymphocytes / Q000166:cytology*; D011971:Receptors, Immunologic / Q000032:analysis; D012756:Sheep / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018801:CD2 Antigens; D018956:CD5 Antigens; D007075:Immunoglobulin M; D011971:Receptors, Immunologic