Continuous epitopes of human lymphotoxin and their topographies.
Human lymphotoxin (hLT or TNF-beta) is a lymphokine that is structurally and functionally related to tumor necrosis factor alpha (TNF-alpha). The continuous epitopes of hLT were located by examining the cross-reaction between rabbit anti-hLT antibody and peptides derived from proteolytic digestion and chemical synthesis. Three antigenic sites, corresponding to residues 40-48, 83-94 and 139-147, of the protein sequence, were located by this approach. Since residues 49-57 also exhibited trace antigenicity, but residues 45-52 displayed no reaction, the whole peptide fragment consisting of residues 40-57 might be necessary for antigenicity. A comparison of the antigenic determinants with the loop structures obtained from X-ray crystallographic studies of hLT showed that all of the epitopes are found on or adjacent to functionally important domains.
Yao
Z J
ZJ
Bioprocessing Technology Unit, National University of Singapore, Republic of Singapore.
Kao
M C
MC
Loh
K C
KC
Chung
M C
MC
D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D000939:Epitopes / Q000737:chemistry*; D006801:Humans; D008233:Lymphotoxin-alpha / Q000276:immunology*; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011994:Recombinant Proteins / Q000276:immunology; D012995:Solubility; D013329:Structure-Activity Relationship; D014867:Water / Q000737:chemistry
D000939:Epitopes; D008233:Lymphotoxin-alpha; D011994:Recombinant Proteins; D014867:Water