title: Autoantibodies against a novel DNA-binding protein: DNA-protein interaction as a requisite for expression of antigenic reactivity.
abstract: A novel DNA-binding protein complex, the HB complex, has been characterized by means of autoantibodies. Three proteins of 9000, 7500, and 7000 Da constitute the HB complex. The 9000- and 7500-Da proteins are phosphorylated. Autoantibodies recognize the 7000-Da protein when it is bound to DNA. No reactivity against any protein was observed when the complex was dissociated from DNA. The three proteins are acidic (pI 5-6.2), and the complex was able to bind to synthetic double-stranded DNAs of different composition.
authors.affiliation: Department of Immunology, Hospital de Sant Pau, Universitat Autonoma de Barcelona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Juarez
authors.forename: J L
authors.initials: JL
authors.lastname: Vila
authors.forename: M
authors.initials: M
authors.lastname: Agusti
authors.forename: C
authors.initials: C
authors.lastname: Gelpi
authors.forename: M J
authors.initials: MJ
authors.lastname: Amengual
authors.forename: S
authors.initials: S
authors.lastname: Cardona
authors.forename: J L
authors.initials: JL
authors.lastname: Rodriguez-Sanchez
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000276:immunology*; D002417:Cattle; D005591:Chemical Fractionation; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000276:immunology* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans
chemical_list: D001323:Autoantibodies; D004268:DNA-Binding Proteins; D000939:Epitopes; D004247:DNA