[Specific antibody assay for genotyping of hepatitis C virus].
Based on their nucleotide sequences and the deduced amino acid sequences, most hepatitis C virus (HCV) isolates have been classified into two genotypes, type 1 (subtype 1a and 1b) and type 2 (subtype 2a and 2b). Using part of the putative NS4 protein region (amino acids No. 1676-1760) as antigens, an enzyme-linked immunosorbent assay (ELISA) was developed. The results of genotyping of HCVs in chronic hepatitis patients by this ELISA system were almost completely consistent with those by PCR. The genotype 1 HCV infected patients showed a poor response to interferon (IFN) therapy. In contrast, genotype 2 HCV infected patients showed good response. The results suggest that genotype 1 and 2 HCVs possess different biological properties.
Kohara
M
M
Department of Microbiology, Tokyo Metropolitan Institute of Medical Science.
D000595:Amino Acid Sequence; D000918:Antibody Specificity; D005838:Genotype; D016174:Hepacivirus / Q000145:classification* / Q000235:genetics / Q000276:immunology; D006508:Hepatitis Antibodies / Q000032:analysis; D006526:Hepatitis C / Q000382:microbiology / Q000628:therapy; D018937:Hepatitis C Antibodies; D006801:Humans; D007372:Interferons / Q000627:therapeutic use; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D014759:Viral Envelope Proteins; D007372:Interferons