Large platelets continue to circulate in an activated state after myocardial infarction.
This study was intended to investigate the actual platelet activation status after an acute coronary event. The activation status of circulating platelets was assayed directly by measuring the membrane activation markers CD62 and CD63 with the Düsseldorf III flow cytometry test in 22 patients with the diagnosis of acute myocardial infarction during the 48-h observation period following the acute event. The number of activated, marker-positive sample platelets was significantly increased in the post-MI patients: CD62: 5.8% x 2.25 +/- 1 vs. 3.5% x 2.32 +/- 1, P < or = 0.05; CD63: J8.7% x 1.77 +/- 1 vs. 4.6% x 2.16 +/- 1, P < or = 0.00.1. The platelet volume and count were concomitantly increased (12.1 +/- 2.4 fl/ 236 +/- 90 x 10(3) microliters-1 compared to 8.3 +/- 1.6 fl/ 187 +/- 42 x 10(3) microliters-1) in the control group. Particularly large platelets were identified as being activated documented by the exponential increase in the difference in CD63-binding sites per sample platelet above the 90%-percentile and below the 10%-percentile of the volume distribution: delta + 1341 +/- 903 (MI patients) vs. delta + 276 +/- 126 (controls), P < or = 0.00.1. Significant creatine kinase elevation and decrease in platelet count was found in the non-survivor subset (n = 5). We conclude that predominantly large platelets continue to circulate in an activated state after MI. This study provides direct evidence that the assumption of an increased thrombotic potential becomes operative in vivo in MI patients.(ABSTRACT TRUNCATED AT 250 WORDS)
Schultheiss
H P
HP
Diabetes Research Institute, Heinrich Heine University, Duesseldorf, Germany.
Tschoepe
D
D
Esser
J
J
Schwippert
B
B
Roesen
P
P
Nieuwenhuis
H K
HK
Schmidt-Soltau
C
C
Strauer
B
B
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D001665:Binding Sites; D001792:Blood Platelets / Q000473:pathology*; D048429:Cell Size; D003402:Creatine Kinase / Q000097:blood; D003430:Cross-Sectional Studies; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood* / Q000503:physiopathology; D019007:P-Selectin; D015539:Platelet Activation / Q000502:physiology*; D010980:Platelet Membrane Glycoproteins / Q000032:analysis; D012189:Retrospective Studies; D060149:Tetraspanin 30
D015703:Antigens, CD; C554586:CD63 protein, human; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D060149:Tetraspanin 30; D003402:Creatine Kinase