Is asparagine-linked protein glycosylation an obligatory requirement for angiogenesis?
Dependence of protein N-glycosylation on capillary endothelial cell proliferation has been studied. Amphomycin, a potent N-glycosylation inhibitor, inhibited capillary endothelial cell proliferation in a dose-dependent manner. beta-Agonist isoproterenol as well as other intracellular cAMP enhancing agents, viz. cholera toxin, prostaglandin E1 and 8Br-cAMP, also enhanced capillary endothelial cell proliferation. In addition to cell proliferation, isoproterenol also enhanced protein glycosylation in these cells. Isoproterenol effect was mediated by beta-adrenoreceptors, as it got reduced on pre-treatment of cells with either atenolol or ICI 118, 551 or propranolol. Furthermore, isoproterenol stimulation of protein glycosylation by exogenous dolichyl monophosphate and its inhibition by tunicamycin (GlcNAc-1P transferase inhibitor) supported the concept that isoproterenol specifically stimulated protein N-glycosylation event(s) in the cell.
Banerjee
D K
DK
Department of Biochemistry, School of Medicine, University of Puerto Rico, San Juan 00936-5067.
Vendrell-Ramos
M
M
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000313:Adrenal Medulla / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000527:Alprostadil / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001216:Asparagine; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D006031:Glycosylation / Q000187:drug effects; D007545:Isoproterenol / Q000494:pharmacology; D055666:Lipopeptides; D009389:Neovascularization, Pathologic; D009842:Oligopeptides / Q000494:pharmacology; D011499:Protein Processing, Post-Translational / Q000187:drug effects*
D000319:Adrenergic beta-Antagonists; D000900:Anti-Bacterial Agents; D055666:Lipopeptides; D009842:Oligopeptides; D015124:8-Bromo Cyclic Adenosine Monophosphate; C004423:amphomycin; D001216:Asparagine; D002772:Cholera Toxin; D000242:Cyclic AMP; D000527:Alprostadil; D007545:Isoproterenol