Immunolocalization of stratum corneum chymotryptic enzyme in human skin and oral epithelium with monoclonal antibodies: evidence of a proteinase specifically expressed in keratinizing squamous epithelia.
Stratum corneum chymotryptic enzyme (SCCE) is a recently discovered serine proteinase, which has been purified from human plantar stratum corneum. Evidence has been presented that it may play a role in the terminal stages of epidermal turnover, especially in desquamation. Two mouse monoclonal antibodies (MAb) were raised, TE4b and TE9b, that reacted specifically with SCCE in immunoprecipitation, immunoblotting, and gel-exclusion chromatography. When used in immunohistochemical experiments with the peroxidase-anti-peroxidase method, both MAb detected an antigen located in high suprabasal keratinocytes of the epidermis in normal human skin and at the vermilion border of the lip, with maximal staining of the stratum granulosum. In the hair follicles the MAb reacted with the inner root sheet only. In human oral mucosa the MAb stained the high suprabasal epithelial cells of the hard palate. This is a site where the epithelium forms an orthokeratotic stratum corneum. There was no specific staining of the epithelium of the lip mucosa or the buccal mucosa, where the epithelium does not form a stratum corneum under non-pathological conditions. A correlation therefore seems to exist between the presence of SCCE in high suprabasal cells and the ability of the epithelium to form an orthokeratotic cornified layer. We suggest that SCCE is specifically expressed in keratinizing squamous epithelia and that its expression may be part of the terminal differentiation program of this type of epithelium. These results also give further support to the idea that SCCE may play a role in the turnover and/or formation of the stratum corneum.
Sondell
B
B
Department of Dermatology, University of Umeå, Sweden.
Thornell
L E
LE
Stigbrand
T
T
Egelrud
T
T
D002454:Cell Differentiation; D004848:Epithelium / Q000201:enzymology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007610:Kallikreins; D015603:Keratinocytes / Q000201:enzymology; D007633:Keratins / Q000096:biosynthesis*; D008297:Male; D009061:Mouth Mucosa / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000276:immunology / Q000302:isolation & purification*; D012867:Skin / Q000201:enzymology*; D014018:Tissue Distribution
D007633:Keratins; C082472:KLK7 protein, human; D007610:Kallikreins; D012697:Serine Endopeptidases