A general purification procedure for chemically synthesized oligoribonucleotides.
A general procedure for the purification of chemically synthesized oligoribonucleotides is reported. Purification based on the use of a single reverse-phase HPLC column with buffer systems of differing ion-pairing capacity is described. These methods have been applied to the preparation of a series of RNAs which range in size from 10 to 46 nucleotides. The yields obtained are high, up to 53% (based on isolated product compared to those obtained from final trityl assay). The purity of the isolated material is 96-99%. Thus with this general procedure, milligram quantities of extremely pure RNA can be efficiently obtained.
Murray
J B
JB
Department of Genetics, University of Leeds, United Kingdom.
Collier
A K
AK
Arnold
J R
JR
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D018360:Crystallography, X-Ray; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000302:isolation & purification*; D012313:RNA / Q000138:chemical synthesis / Q000302:isolation & purification*
D009843:Oligoribonucleotides; D012313:RNA