Subcellular distribution and immunological detection of retrograde axonally transported proteins in acrylamide and diabetic neuropathies.
Neuropathies produced by both acrylamide- and streptozotocin-induced diabetes in the rat are accompanied by a deficit in the retrograde axonal transport of a defined group of proteins that can be visualized on two-dimensional polyacrylamide gels. In this work, these proteins are identified as being primarily soluble and being absent from rat brain. They are not immunologically related to the major retrogradely transported protein synaptophysin. Polyclonal antiserum to the proteins was produced in mice and used to confirm the reduction in their retrograde transport in sciatic nerve of diabetic and acrylamide-treated rats.
Hargreaves
A J
AJ
Department of Pharmacology and Therapeutics, University of Liverpool, UK.
McLean
W G
WG
D020106:Acrylamide; D000178:Acrylamides; D000818:Animals; D001370:Axonal Transport; D015153:Blotting, Western; D001921:Brain / Q000503:physiopathology*; D002458:Cell Fractionation; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D003929:Diabetic Neuropathies / Q000503:physiopathology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D008928:Mitochondria / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000503:physiopathology*; D013347:Subcellular Fractions / Q000378:metabolism; D013570:Synaptic Membranes / Q000378:metabolism
D000178:Acrylamides; D009419:Nerve Tissue Proteins; D020106:Acrylamide