Direct detection of the Philadelphia chromosome in CD20-positive lymphocytes in chronic myeloid leukemia by tri-color immunophenotyping/FISH.
Six patients with previously diagnosed chronic myelogenous leukemia (CML) were studied by a tri-color immunophenotyping/FISH method for direct determination of the Philadelphia (Ph) chromosome in B and T lymphocytes. Two patients had involvement of CD20-positive lymphocytes. CD3-positive lymphocytes in all patients were negative for the Ph chromosome.
Torlakovic
E
E
Department of Laboratory Medicine and Pathology, University of Minnesota Hospital and Clinics, Minneapolis.
Litz
C E
CE
McClure
J S
JS
Brunning
R D
RD
D015703:Antigens, CD / Q000032:analysis; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000175:diagnosis* / Q000276:immunology; D008214:Lymphocytes / Q000201:enzymology; D009195:Peroxidase / Q000378:metabolism
D015703:Antigens, CD; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte; D017252:CD3 Complex; D009195:Peroxidase; D004253:DNA Nucleotidylexotransferase