New modified substrates for discriminating between human DNA polymerases alpha and epsilon.
Two 2'-deoxynucleoside 5'-alpha-methylenephosphonyl-beta, gamma-diphosphates were synthesized. They were incorporated into the DNA chain by DNA polymerase alpha from human placenta. Meanwhile, they were not recognized by DNA polymerase epsilon and beta of the same origin as well as by reverse transcriptases from human immunodeficiency virus and avian myeloblastosis virus.
Jasko
M V
MV
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Semizarov
D G
DG
Victorova
L S
LS
Mozzherin DJu
Krayevsky
A A
AA
Kukhanova
M K
MK
D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004257:DNA Polymerase II / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006678:HIV / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013379:Substrate Specificity
D009711:Nucleotides; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase