Inhibitory effects of enrichment media on the Accuprobe test for Listeria monocytogenes.
During an evaluation of the Accuprobe kit for the detection of Listeria monocytogenes, some of the enrichment media used were found to interfere with the test. Microscopic examination during the lysis step of the test revealed that media containing high salt greatly reduced or prevented cell lysis. This prevented the probe from binding to the cellular RNA, resulting in false-negative results.
Partis
L
L
Australian Government Analytical Laboratories, Pymble, New South Wales.
Newton
K
K
Murby
J
J
Wells
R J
RJ
D001431:Bacteriological Techniques; D001433:Bacteriolysis; D003470:Culture Media; D005188:False Negative Reactions; D008089:Listeria monocytogenes / Q000302:isolation & purification*; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D015347:RNA Probes; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D011933:Reagent Kits, Diagnostic
D003470:Culture Media; D015347:RNA Probes; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D011933:Reagent Kits, Diagnostic