Clonogenic potential of myeloid leukaemia cells in vitro is restricted to leukaemia cells expressing the CD34 antigen.
Cells from patients with acute myeloid leukaemia (AML) or chronic myeloid leukaemia (CML) were separated into CD34-enriched and CD34-depleted subpopulations. The clonogenic capacities of these two subpopulations were then compared to each other and to the original unseparated cell population. In every study, the CD34-enriched subpopulation demonstrated a substantial increase in clonogenicity in vitro in comparison with the original cell population, while the reverse was the case for the CD34-depleted subpopulations. For reasons not clear at present, the enrichment for clonogenic cells far exceeded the enrichment for cells expressing the CD34 antigen. Additionally, the clonogenic potential was found to be unrelated to the level of myc expression in the various cell populations.
Yin
M
M
University of Cincinnati, Ohio.
Silvestri
F F
FF
Banavali
S D
SD
Gopal
V
V
Hulette
B C
BC
Kuvelkar
R B
RB
Young
A N
AN
Mayers
G
G
Preisler
H D
HD
D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D002455:Cell Division; D002469:Cell Separation; D015870:Gene Expression; D016259:Genes, myc; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000276:immunology; D007951:Leukemia, Myeloid / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D014410:Tumor Stem Cell Assay
D015703:Antigens, CD; D018952:Antigens, CD34