title: Immunohistochemical analysis of tissues regenerated from within periodontal defects treated with expanded polytetrafluoroethylene membranes.
abstract: Immunocytochemical analysis was carried out on samples of 5-, 6-, and 9-week old regenerated soft tissue taken from healing periodontal defects treated by guided tissue regeneration using expanded polytetrafluorethylene (ePTFE) membranes. A panel of monoclonal and polyconal antibodies to cytokeratins, vimentin, and collagen was used to label cells and collagen types I, III, and IV. Epithelium was identified in 7 of the 9 samples examined, in addition to mesenchymal cells staining positively for vimentin and co-distribution of collagen types I, III, and IV in all samples. Clinical observations indicated that exposure of the ePTFE membranes during healing was a frequent occurrence, and the presence and quantity of epithelium found within the healing defect beneath the membrane may be related to the extent to which this occurs.
authors.affiliation: Department of Periodontology, United Medical School, Guy's Hospital, London, UK.
authors.forename: S
authors.initials: S
authors.lastname: Pritlove-Carson
authors.forename: R M
authors.initials: RM
authors.lastname: Palmer
authors.forename: P D
authors.initials: PD
authors.lastname: Floyd
authors.forename: P M
authors.initials: PM
authors.lastname: Morgan
mesh_terms: D000328:Adult; D003094:Collagen / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D016557:Guided Tissue Regeneration, Periodontal; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000378:metabolism; D010510:Periodontal Diseases / Q000378:metabolism*; D011138:Polytetrafluoroethylene; D014746:Vimentin / Q000378:metabolism
chemical_list: D014746:Vimentin; D007633:Keratins; D005340:Fibrinogen; D011138:Polytetrafluoroethylene; D003094:Collagen