Existence of an optimum expression level for secretion of foreign proteins in yeast.
Wittrup
K D
KD
Department of Chemical Engineering, University of Illinois, Urbana 61801.
Robinson
A S
AS
Parekh
R N
RN
Forrester
K J
KJ
D007611:Aprotinin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D001692:Biological Transport; D003001:Cloning, Molecular / Q000379:methods*; D004721:Endoplasmic Reticulum; D004921:Erythropoietin / Q000096:biosynthesis / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005813:Genes, Synthetic; D005822:Genetic Vectors; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis / Q000378:metabolism; D017510:Protein Folding; D011499:Protein Processing, Post-Translational; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013997:Time Factors
D010982:Platelet-Derived Growth Factor; D011993:Recombinant Fusion Proteins; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor; D007611:Aprotinin