The structure of the O-specific polysaccharide of Salmonella arizonae O62.
The O-specific polysaccharide was liberated by mild acid hydrolysis of the lipopolysaccharide (LPS) isolated from S. arizonae O62 by phenol-water extraction. The branched hexasaccharide repeating-unit of the O-specific chain of the O62 LPS contained L-rhamnose, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-2-deoxy-D-galacturonic acid in molar ratios of 4:1:1. On the basis of methylation analysis, 1H and 13C NMR spectroscopy, including 2D shift-correlated (COSY) and 1D NOE spectroscopy, the following structure for the repeating unit of the O-specific polysaccharide was established: [formula: see text]
Vinogradov
E V
EV
M.M. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Knirel
Y A
YA
Kochetkov
N K
NK
Schlecht
S
S
Mayer
H
H
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D008401:Gas Chromatography-Mass Spectrometry; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012476:Salmonella arizonae / Q000737:chemistry / Q000276:immunology*
D019081:O Antigens; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D006859:Hydrogen