Sustained c-kit expression in a human erythroleukemia cell line (HEL) after megakaryocytic differentiation induced by 12-O-tetradecanoylphorbol-13-acetate (TPA).
Changes in c-kit proto-oncogene expression were examined in a human erythroleukemia cell line, HEL, during 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced megakaryocytic differentiation. When HEL cells were treated with 10(-7) M TPA, glycophorin A expression and hemoglobin synthesis were reduced, while the expression of GP IIb/IIIa was induced in association with the morphological changes. Northern blot analysis showed that, during this megakaryocytic differentiation of HEL cells, c-kit mRNA expression persisted even after there was an apparent reduction in c-myc mRNA. This finding supports the idea that the expression of c-kit, a marker of primitive hematopoietic progenitors, may persist along with differentiation toward a megakaryocytic lineage.
Bakić
M
M
Department of Hematology-Oncology, University of Tokyo, Japan.
Ozawa
K
K
Uemura
N
N
Nishikawa
M
M
Tojo
A
A
Takahashi
K
K
Tani
K
K
Zaike
Y
Y
Ikebuchi
K
K
Asano
S
S
D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D017931:DNA Primers; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006021:Glycophorins / Q000096:biosynthesis; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D008533:Megakaryocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis; D016133:Polymerase Chain Reaction; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000096:biosynthesis*; D019009:Proto-Oncogene Proteins c-kit; D011519:Proto-Oncogenes / Q000187:drug effects; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis / Q000096:biosynthesis*; D016184:Receptors, Colony-Stimulating Factor / Q000032:analysis / Q000096:biosynthesis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D006021:Glycophorins; D006454:Hemoglobins; C000715470:MAS1 protein, human; D016376:Oligonucleotides, Antisense; D010980:Platelet Membrane Glycoproteins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D016184:Receptors, Colony-Stimulating Factor; D019009:Proto-Oncogene Proteins c-kit; D020794:Receptor Protein-Tyrosine Kinases; D013755:Tetradecanoylphorbol Acetate