Inhibitory effect of fatty acids on 8-hydroxydeoxyguanosine formation in calf thymus DNA treated with bleomycin-Fe(II).
The effect of fatty acids on 8-hydroxydeoxyguanosine (8-OH-dG) formation in calf thymus DNA treated with bleomycin (BLM)-Fe(II) in vitro was studied. The formation of 8-OH-dG was greatly reduced in the presence of stearic acid (18:0) or oleic acid (18:1), however, it increased to the control level with an increase in the number of unsaturated bonds of fatty acids (18:2, 18:3). This increase in 8-OH-dG formation may have resulted from the indirect oxidation of DNA by the peroxidized unsaturated fatty acids formed by BLM-Fe(II). The inhibitory effect of saturated fatty acids on 8-OH-dG formation was observed with those possessing more than 12 methylene chains. Stearic acid also inhibited peroxidation of unsaturated fatty acids (18:2, 18:3) treated with BLM-Fe(II), however, it had no effect on ribose degradation of DNA treated with BLM-Fe(II). These results suggest that different active oxygen species are probably involved in DNA degradation and in formation of 8-OH-dG in DNA and of peroxidized products in fatty acids.
Kasamatsu
T
T
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Nakagawa
T
T
Kohda
K
K
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D002417:Cattle; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000494:pharmacology; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D010084:Oxidation-Reduction; D010545:Peroxides / Q000737:chemistry; D017392:Thiobarbituric Acid Reactive Substances / Q000737:chemistry; D013950:Thymus Gland / Q000737:chemistry*
D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D016859:Lipoxygenase Inhibitors; D010545:Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D001761:Bleomycin; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA; D003849:Deoxyguanosine