Deoxyribonuclease inhibitors produced by streptomycetes.
Streptomyces sp. strain No. A-5838 produces three types of inhibitors of DNase II. Two of them, DNI-2 and DNI-3, were distinguished from the previously reported DNase II inhibitors, 5838-DNI and 5923-DNI, by their inhibitory profiles towards phosphodiesterases. DNI-2 has M(r) 654, and is considered to be a coproporphyrin. DNI-3 is an acidic substance with M(r) about 60,000 as estimated by gel filtration. The inhibitory activities of both inhibitors were shown to be temperature-dependent whereas only that of DNI-2 was pH-dependent.
Suzuki
K
K
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Nagao
K
K
Goto
M
M
Uyeda
M
M
D002850:Chromatography, Gel; D003306:Coproporphyrins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000037:antagonists & inhibitors*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D013302:Streptomyces / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature
D003306:Coproporphyrins; D004791:Enzyme Inhibitors; D003851:Deoxyribonucleases