The relationship of efferent projections from the area postrema to vagal motor and brain stem catecholamine-containing cell groups: an axonal transport and immunohistochemical study in the rat.
The area postrema has been implicated as a major station for the processing of visceral sensory information, involved primarily in eliciting rapid homeostatic responses to fluid and nutrient imbalances. Yet the precise relationship of efferent projections from the area postrema to medullary motor and relay nuclei involved in such functions remains unclear. In this study, axonal transport and immunohistochemical techniques were used to investigate the relationship of efferent projections from the area postrema to vagal motor neurons and medullary catecholamine-containing cell groups in the rat. The results may be summarized as follows: (1) The area postrema gives rise to dense inputs to the commissural and medial parts of the nucleus of the solitary tract. Many of these projections are intimately associated with catecholamine-containing neurons in the A2 and C2 cell groups, including a particularly prominent input to a caudally placed cluster of adrenergic neurons (the C2d cell group) in the dorsal aspect of the medial part of the nucleus of the solitary tract. (2) The area postrema provides a dense input to the external lateral part of the parabrachial nucleus. (3) The area postrema does not project significantly to vagal motor neurons in either the dorsal motor nucleus or the nucleus ambiguus, although the possibility for inputs to distal dendrites of dorsal vagal motor neurons cannot be excluded. (4) En route to the parabrachial nucleus, axons of area postrema neurons traverse the regions of the A1, C1 and A5 cell groups, although these fibers make few arborizations, suggesting little functional contact. Together, these results suggest that sensory information received by the area postrema is dispatched to a restricted set of neurons in the commissural, medial, and dorsal parts of the nucleus of the solitary tract, most probably including catecholamine-containing cells in the A2, C2, and C2d cell groups, and to the external lateral portion of the parabrachial nucleus. The targets of area postrema projections are, in turn, in a position to effect adaptive changes in the activities of hypothalamic neurosecretory neurons, vagal motor neurons, and limbic forebrain regions in response to perturbations in fluid and nutrient homeostasis.
Cunningham
E T
ET
Laboratory of Neuronal Structure and Function, Salk Institute for Biological Studies, San Diego, CA 92186.
Miselis
R R
RR
Sawchenko
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PE
D000818:Animals; D001370:Axonal Transport / Q000502:physiology*; D001933:Brain Stem / Q000166:cytology / Q000502:physiology*; D002395:Catecholamines / Q000378:metabolism*; D002552:Cerebral Ventricles / Q000166:cytology / Q000502:physiology*; D002772:Cholera Toxin; D006735:Horseradish Peroxidase; D007150:Immunohistochemistry; D007478:Iontophoresis; D008526:Medulla Oblongata / Q000166:cytology / Q000502:physiology; D009046:Motor Neurons / Q000502:physiology; D009476:Neurons, Efferent / Q000502:physiology*; D010835:Phytohemagglutinins; D011149:Pons / Q000166:cytology / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017552:Solitary Nucleus / Q000166:cytology / Q000502:physiology; D014446:Tyrosine 3-Monooxygenase / Q000276:immunology / Q000378:metabolism; D014630:Vagus Nerve / Q000166:cytology / Q000502:physiology*
D002395:Catecholamines; D010835:Phytohemagglutinins; C052635:cholera toxin, B subunit-horseradish peroxidase; C100156:leukoagglutinins, plants; D002772:Cholera Toxin; D006735:Horseradish Peroxidase; D014446:Tyrosine 3-Monooxygenase