Bluetongue virus: production and study of viral antigen for serological diagnosis.
A soluble antigen, produced from the culture supernatant of VERO cells infected with bluetongue virus serotype 4 (BTV-S4) and concentrated by sequential ultrafiltration with membranes with cut-off values 10(3) and 25 x 10(3) NMWP, showed complete identity to standard antigens when compared by agar gel immunodiffusion (AGID) and SDS-PAGE profiles, revealing that the main protein component responsible for the AGID reaction has a molecular weight of about 60 kDa corresponding probably to the NS1 protein.
Arita
G M
GM
Laboratorio de Referência Animal, LARA/Campinas, MARA, São Paulo, Brazil.
Gatti
M S
MS
Deak
J G
JG
de Castro
A F
AF
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000737:chemistry / Q000276:immunology*; D001819:Bluetongue / Q000175:diagnosis*; D001820:Bluetongue virus / Q000737:chemistry / Q000254:growth & development / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005779:Immunodiffusion; D012756:Sheep; D014709:Vero Cells; D014776:Virus Cultivation
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes