cis-acting elements in 5'-flanking region of rat alpha-fetoprotein mediating retinoic acid responsiveness.
A distal RA responsive cis-acting element has been identified in the 5'-flanking region of the alpha-fetoprotein gene by transfection of different deletion mutants of AFP-CAT fusion gene. The retinoic acid receptor specifically binds to this RA responsive cis-acting element in mobility shift assays. Furthermore, this cis-acting element functions in exogenous TK promoter in transient cotransfection assays. This study suggests a role for the RA responsive cis-acting element in the RA induction of alpha-fetoprotein gene expression.
Liu
Y
Y
Department of Biochemistry, College of Medicine, University of Vermont, Burlington 05405.
Chen
H
H
Dong
J M
JM
Chiu
J F
JF
D000818:Animals; D001483:Base Sequence; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D051381:Rats; D018168:Receptors, Retinoic Acid / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D000509:alpha-Fetoproteins / Q000235:genetics*
D009838:Oligodeoxyribonucleotides; D018168:Receptors, Retinoic Acid; D000509:alpha-Fetoproteins; D014212:Tretinoin; D015500:Chloramphenicol O-Acetyltransferase