title: Patterns of differentiation in extraosseous Ewing's sarcoma cells. An in vitro study.
abstract: BACKGROUND
  In vitro, tissue culture-associated differentiation assays have facilitated the identification of multiple tumor-cell types.
  
  
  METHODS
  We have investigated the capability of differentiation of three extraosseous Ewing's sarcoma cell lines toward a neural and muscular direction by in vitro stimulation with dibutyryl cyclic adenosine-monophosphate (db cAMP) and 5-azacytidine, respectively.
  
  
  RESULTS
  Elongation of cytoplasmic processes and increase of neural markers chromogranin, S-100 protein, and glial fibrillary acidic protein were observed after db cAMP treatment of these lines and neurosecretory granules as well as myelin figures were demonstrated ultrastructurally. These results support the existence of several pathways of neural differentiation in vitro--neuroblastic, Schwannian, and central glial--in stages of maturation more advanced than those previously reported in Ewing's sarcoma of bone. The cell lines showed no definitive myoblastic differentiation after 5-azacytidine treatment.
  
  
  CONCLUSIONS
  These data suggest that these three extraosseous Ewing's sarcoma cell lines configurate a heterogeneous group of tumors with respect to capability of differentiation into the neural lineage, arrested at more advanced stages of neural crest development than Ewing's sarcoma of bone and without capability of myoblastic differentiation with 5-azacytidine.
authors.affiliation: Department of Pathology, Medical School, University of Valencia, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Noguera
authors.forename: S
authors.initials: S
authors.lastname: Navarro
authors.forename: A
authors.initials: A
authors.lastname: Peydró-Olaya
authors.forename: A
authors.initials: A
authors.lastname: Llombart-Bosch
mesh_terms: D000008:Abdominal Neoplasms / Q000473:pathology; D000293:Adolescent; D000328:Adult; D001374:Azacitidine / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008297:Male; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D012512:Sarcoma, Ewing / Q000473:pathology* / Q000648:ultrastructure; D012983:Soft Tissue Neoplasms / Q000473:pathology* / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
chemical_list: D003994:Bucladesine; D001374:Azacitidine