Simultaneous culture for adenovirus, cytomegalovirus, and herpes simplex virus in same shell vial by using three-color fluorescence.
A simultaneous triple culture for adenovirus, cytomegalovirus, and herpes simplex virus, using three different fluorochromes for virus detection and differentiation in the same shell vial, was developed. In evaluations the triple culture performed comparably to separate cultures, required less specimen volume, and was less expensive to perform.
Brumback
B G
BG
Virology Department, American Medical Laboratories, Inc., Chantilly, Virginia 22021.
Wade
C D
CD
D000257:Adenoviridae Infections / Q000175:diagnosis* / Q000821:virology; D000260:Adenoviruses, Human / Q000254:growth & development / Q000302:isolation & purification*; D003587:Cytomegalovirus / Q000254:growth & development / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000821:virology; D005456:Fluorescent Dyes; D006561:Herpes Simplex / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D009928:Organ Specificity; D011237:Predictive Value of Tests; D018139:Simplexvirus / Q000254:growth & development / Q000302:isolation & purification*; D013194:Staining and Labeling; D014776:Virus Cultivation / Q000191:economics / Q000295:instrumentation / Q000379:methods*
D005456:Fluorescent Dyes