[Effects of linesinine on slow action potentials in myocardium and slow inward current in canine cardiac Purkinje fibers].
Standard microelectrode and two-microelectrodes voltage clamp techniques were used to study the effects of linesinine (Lien), an alkaloid extracted from the green seed embryo of Nelumbo nucifera Gaertn, on slow action potentials (AP) and slow inward current (Isi). Lien 10-100 mumol/L was shown to concentration-dependently decrease the action potential amplitude (APA), the maximal velocity of phase 0 depolarization (Vmax) and prolong the sinus cycle length (SCL) of slow AP in isolated sinoatrial node (SAN) pacemaker cells of rabbits. On both slow APs of rabbit SAN cells and guinea pig papillary muscles partly depolarized by high K+, Bay K 8644 0.03 mumol/L markedly increased the APA and Vmax, which were obviously antagonized by Lien 10 mumol/L. Moreover, Lien 1-100 mumol/L was found to inhibit the Isi of canine cardiac Purkinje fiber in concentration-dependent manner. Lien 3 and 100 mumol/L reduced the peak value of Isi by 14% and 88% respectively. The results suggest that Lien possesses calcium antagonistic effects.
Wang
J L
JL
Department of Pharmacology, Tongji Medical University, Wuhan.
Nong
Y
Y
Xia
G J
GJ
Yao
W X
WX
Jiang
M X
MX
D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000037:antagonists & inhibitors; D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D015220:Calcium Channels / Q000187:drug effects; D004285:Dogs; D005260:Female; D006168:Guinea Pigs; D006321:Heart / Q000502:physiology; D007546:Isoquinolines / Q000494:pharmacology*; D008297:Male; D010636:Phenols / Q000494:pharmacology*; D011690:Purkinje Fibers / Q000187:drug effects*; D011817:Rabbits; D051381:Rats
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D007546:Isoquinolines; D010636:Phenols; C080095:liensinine; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester