An immortalised murine hypothalamic neuronal cell, GT1-7, expresses functional histamine H1 receptors.
Histamine, acting via H1 receptors, dose-dependently stimulated [3H]inositol phosphate production in GT1-7 neuronal cells. GT1-7 cells also responded to Substance P but not to other neuroactive drugs tested. Acute histamine pretreatment desensitised the histamine-induced response, resulting in a reduction in the maximal response and a slower time-course of [3H]-inositol phosphate production. The desensitisation phenomenon was reversible, with full recovery by 2 h.
Zamani
M R
MR
School of Biological Sciences, Division of Neuroscience, University of Manchester, UK.
Dupere
J R
JR
Bristow
D R
DR
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002461:Cell Line, Transformed; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology*; D006633:Histamine Antagonists; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D007031:Hypothalamus; D007295:Inositol Phosphates / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D009478:Neuropeptide Y / Q000494:pharmacology; D010880:Piperidines / Q000494:pharmacology; D011744:Pyrimidinones / Q000494:pharmacology; D011899:Ranitidine / Q000494:pharmacology; D011969:Receptors, Histamine H1 / Q000096:biosynthesis / Q000502:physiology*; D012701:Serotonin / Q000494:pharmacology; D013373:Substance P / Q000494:pharmacology*; D014316:Tritium
D006633:Histamine Antagonists; D006634:Histamine H1 Antagonists; D007295:Inositol Phosphates; D009478:Neuropeptide Y; D010880:Piperidines; D011744:Pyrimidinones; D011969:Receptors, Histamine H1; D014316:Tritium; D012701:Serotonin; D013373:Substance P; D018698:Glutamic Acid; D006632:Histamine; D011899:Ranitidine; D002217:Carbachol; C049054:temelastine; C052075:thioperamide