title: Local proliferation of fibroblast-like synoviocytes contributes to synovial hyperplasia. Results of proliferating cell nuclear antigen/cyclin, c-myc, and nucleolar organizer region staining.
abstract: OBJECTIVE
  To test the hypothesis that local proliferation contributes significantly to the hyperplasia of rheumatoid synovium.
  
  
  METHODS
  Immunohistologic and chemical staining was used to identify 3 markers of cell proliferation: proliferating cell nuclear antigen, c-myc proto-oncogene, and nucleolar organizer regions. Synovium from 21 patients with rheumatoid arthritis, 34 with degenerative joint disease, and 7 with joint trauma was examined.
  
  
  RESULTS
  All 3 markers indicated substantial, active proliferation of synovial lining cells in synovium with hyperplasia. Proliferating cells showed type I procollagen immunoreactivity but were negative for CD68, a monocyte/macrophage marker. Proliferation was greater in rheumatoid arthritis than in the other conditions evaluated.
  
  
  CONCLUSIONS
  In situ proliferation of fibroblast-like synoviocytes in the synovium lining contributes considerably to the increase in cell numbers in rheumatoid synovium.
authors.affiliation: Department of Cell Biology and Anatomy, Oregon Health Sciences University, Casey Eye Institute, Portland 97201.
authors.forename: Z
authors.initials: Z
authors.lastname: Qu
authors.forename: C H
authors.initials: CH
authors.lastname: Garcia
authors.forename: L M
authors.initials: LM
authors.lastname: O'Rourke
authors.forename: S R
authors.initials: SR
authors.lastname: Planck
authors.forename: M
authors.initials: M
authors.lastname: Kohli
authors.forename: J T
authors.initials: JT
authors.lastname: Rosenbaum
mesh_terms: D000328:Adult; D000368:Aged; D001324:Autoantigens / Q000378:metabolism; D002455:Cell Division; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000378:metabolism*; D009697:Nucleolus Organizer Region / Q000648:ultrastructure*; D018809:Proliferating Cell Nuclear Antigen; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D013194:Staining and Labeling; D013583:Synovial Membrane / Q000378:metabolism* / Q000473:pathology*
chemical_list: D001324:Autoantigens; C000715470:MAS1 protein, human; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc