Expression of rat brain nitric oxide synthase in baculovirus-infected insect cells and characterization of the purified enzyme.
Rat brain nitric oxide synthase was expressed to a high level in baculovirus-infected insect cells and purified to apparent homogeneity by affinity chromatography. The enzyme had a specific activity of approximately 1 mumol of citrulline.min-1.mg of protein-1 and contained 0.93, 0.45, 0.18 and 0.23 mol of haem, (6R)-5,6,7,8-tetrahydro-L-biopterin (H4biopterin), FAD and FMN per mol of subunit respectively.
Harteneck
C
C
Institut für Pharmakologie, Freie Universität Berlin, Germany.
Klatt
P
P
Schmidt
K
K
Mayer
B
B
D000594:Amino Acid Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D016367:Baculoviridae / Q000201:enzymology* / Q000235:genetics*; D001921:Brain / Q000201:enzymology*; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression; D007700:Kinetics; D019001:Nitric Oxide Synthase; D010053:Ovary / Q000166:cytology / Q000821:virology; D051381:Rats; D018411:Spodoptera / Q000821:virology*
D018076:DNA, Complementary; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases