title: Study of the genotoxic activity of five chlorinated propanones using the SOS chromotest, the Ames-fluctuation test and the newt micronucleus test.
abstract: Three short-term assays (the SOS chromotest, the Ames-fluctuation test and the newt micronucleus test) were carried out to evaluate the genotoxicity of five chlorinated propanones identified in several chlorinated waters (monochloropropanone, 1,1-dichloropropanone, 1,3-dichloropropanone, 1,1,1-trichloropropanone and 1,1,3-trichloropropanone). In the SOS chromotest, all the compounds except monochloropropanone were found to induce primary DNA damage in Escherichia coli. With the fluctuation test, all five chloropropanones showed mutagenic activity on Salmonella typhimurium strain TA100. The newt micronucleus assay detected a clastogenic effect on the peripheral blood erythrocytes of Pleurodeles waltl larvae only for 1,3-dichloropropanone and 1,1,3-trichloropropanone. Moreover, two structure-activity relationships are noticeable: (1) chloropropanones with chlorine substituents on both carbon positions (1,3-DCP and 1,1,3-TCP) are by far more genotoxic than chloropropanones substituted only on one carbon position (1,1-DCP and 1,1,1-TCP); (2) the increase of the number of chlorine substituents decreases the mutagenic activity (fluctuation test) of the chlorinated propanones studied.
authors.affiliation: Laboratory of Genetic Toxicology, Pasteur Institute of Lille, France.
authors.forename: F
authors.initials: F
authors.lastname: Le Curieux
authors.forename: D
authors.initials: D
authors.lastname: Marzin
authors.forename: F
authors.initials: F
authors.lastname: Erb
mesh_terms: D000096:Acetone / Q000031:analogs & derivatives / Q000633:toxicity*; D000498:Allyl Compounds / Q000633:toxicity; D000818:Animals; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D006843:Hydrocarbons, Chlorinated; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D010999:Pleurodeles / Q000235:genetics; D013014:SOS Response, Genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D014881:Water Supply
chemical_list: D000498:Allyl Compounds; D006843:Hydrocarbons, Chlorinated; D000096:Acetone; C023891:1,3-dichloro-1-propene