Peptides from multiple regions of the lectin domain of P-selectin inhibiting neutrophil adhesion.
The selectins are a family of three structurally related glycoproteins that are integral components of leukocyte adhesion to the vascular endothelium. Their involvement in the recruitment and extravasation of neutrophils is critical in mounting an inflammatory reaction. The carbohydrate nature of the selectin ligands suggests that the binding regions of the selectins are contained within the lectin-like domains of the selectins. The synthesis and evaluation for inhibition of selectin binding of overlapping peptides of the lectin and adjacent EGF-like domains of P-selectin have been used to identify small peptides that completely inhibit P-selectin-dependent neutrophil adhesion. These peptides span a region of more than 100 amino acids and may define the carbohydrate recognition domain of P-selectin.
Heavner
G A
GA
Centocor, Inc., Malvern, Pennsylvania.
Falcone
M
M
Kruszynski
M
M
Epps
L
L
Mervic
M
M
Riexinger
D
D
McEver
R P
RP
D000595:Amino Acid Sequence; D015703:Antigens, CD; D002241:Carbohydrates / Q000737:chemistry; D004815:Epidermal Growth Factor / Q000737:chemistry; D006801:Humans; D037102:Lectins / Q000737:chemistry*; D007957:Leukocyte Adherence Inhibition Test; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000187:drug effects*; D019007:P-Selectin; D010455:Peptides / Q000737:chemistry*; D010980:Platelet Membrane Glycoproteins / Q000737:chemistry*; D017434:Protein Structure, Tertiary
D015703:Antigens, CD; D002241:Carbohydrates; D037102:Lectins; D019007:P-Selectin; D010455:Peptides; D010980:Platelet Membrane Glycoproteins; D004815:Epidermal Growth Factor