DNFB contact sensitivity (CS) in BALB/c and C3H/He mice: requirement for early-occurring, early-acting, antigen-specific, CS-initiating cells with an unusual phenotype (Thy-1+, CD5+, CD3-, CD4-, CD8-, sIg-, B220+, MHC class II-, CD23+, IL-2R-, IL-3R+, Mel-14-, Pgp-1+, J11d+, MAC-1+, LFA-1+, and Fc gamma RII+).
Immunization of mice for contact sensitivity induces two different antigen-specific Thy-1+ cell activities that are required to act in sequence for elicitation of contact sensitivity. In this study, 2,4-dinitro-1-fluorobenzene contact sensitivity responses in BALB/c and C3H/He mice demonstrated the importance of early-acting and antigen-specific contact sensitivity-initiating cells to recruit the classical, late-acting contact sensitivity effector T cells. Employing in vitro treatment of sensitized cells with monoclonal antibodies to cell surface determinants and then incubation in complement, prior to adoptive cell transfer, the contact sensitivity-initiating cells were shown to have a surface phenotype that is quite unusual for antigen-specific cells [Thy-1+, CD5+, CD3-, CD4-, CD8-, sIg-, B220+, major histocompatibility complex class II-, CD23+, IL-2R-, IL-3R+, Mel-14-, CD44+ (Pgp-1+), J11d+ (HSA+), MAC-1+, LFA-1+, and Fc gamma IIR+], and is quite different from the late-acting, contact sensitivity-effector T cells (Thy-1+, CD5+, CD3+, CD4+, CD8-, sIg-, B220-, major histocompatibility complex class II-, CD23-, IL-2R+, IL-3R-, and CD44- (Pgp-1-), J11d-(HSA-), MAC-1-, LFA-1+, Fc gamma IIR-). Contact sensitivity initiation was required for elicitation of late 24-h 2,4-dinitro-1-fluorobenzene contact sensitivity responses, in both BALB/c and C3H/He mice. Moreover, relatively high doses of some monoclonal antibodies [anti-B220 (CD45RA) and anti-CD23 (IgE Fc epsilon II receptor)] were necessary to completely eliminate all contact sensitivity-initiating cells that permitted expression of late contact sensitivity-effector T-cell activity. In contrast, high doses of monoclonal antibody specific for surface determinants of late-acting contact sensitivity effector T cells (anti-CD3 and anti-CD4), when used in high doses similar to anti-B220 and anti-CD23, had no effect on contact sensitivity-initiating cell activity. Our results indicate that two very different antigen-specific Thy-1+ cells are necessary to elicit 2,4-dinitro-1-fluorobenzene contact sensitivity in BALB/c and C3H/He mice.
Ishii
N
N
Department of Dermatology, Yokohama City University School of Medicine, Japan.
Takahashi
K
K
Nakajima
H
H
Tanaka
S
S
Askenase
P W
PW
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000627:therapeutic use; D015703:Antigens, CD / Q000235:genetics*; D000943:Antigens, Differentiation / Q000235:genetics; D000954:Antigens, Surface / Q000235:genetics* / Q000276:immunology; D051927:CD24 Antigen; D017252:CD3 Complex / Q000235:genetics; D015704:CD4 Antigens / Q000235:genetics; D018956:CD5 Antigens; D016827:CD8 Antigens / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics; D003877:Dermatitis, Contact / Q000209:etiology* / Q000276:immunology / Q000473:pathology*; D004139:Dinitrofluorobenzene; D000939:Epitopes / Q000235:genetics*; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D018960:Hyaluronan Receptors; D006968:Hypersensitivity, Delayed / Q000139:chemically induced; D019041:L-Selectin; D017493:Leukocyte Common Antigens; D016169:Lymphocyte Function-Associated Antigen-1 / Q000235:genetics; D016177:Macrophage-1 Antigen / Q000235:genetics; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology*; D008809:Mice, Inbred C3H / Q000276:immunology*; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics; D017455:Receptors, IgE / Q000235:genetics / Q000276:immunology; D017452:Receptors, IgG / Q000235:genetics; D015375:Receptors, Interleukin-2 / Q000235:genetics; D016185:Receptors, Interleukin-3 / Q000235:genetics / Q000276:immunology; D016201:Receptors, Lymphocyte Homing / Q000235:genetics; D018800:Thy-1 Antigens; D013997:Time Factors
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D051927:CD24 Antigen; D017252:CD3 Complex; D015704:CD4 Antigens; D018956:CD5 Antigens; D016827:CD8 Antigens; D002352:Carrier Proteins; C497890:Cd24a protein, mouse; D015815:Cell Adhesion Molecules; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D018960:Hyaluronan Receptors; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; D011947:Receptors, Antigen, B-Cell; D011956:Receptors, Cell Surface; D017455:Receptors, IgE; D017452:Receptors, IgG; D015375:Receptors, Interleukin-2; D016185:Receptors, Interleukin-3; D016201:Receptors, Lymphocyte Homing; D018800:Thy-1 Antigens; D019041:L-Selectin; D004139:Dinitrofluorobenzene; D017493:Leukocyte Common Antigens