Gene regulation by the 5'-untranslated region of the platelet-derived growth factor A-chain.
Platelet-derived growth factor (PDGF) A-chain gene contains a long 5'-untranslated region (5'-UTR) of 850 bp. We evaluated the role of the 5'-UTR by chloramphenicol acetyltransferase (CAT) assay. CAT activity appeared when the fragment +99 bp downstream from the initiation site (+1) was present but disappeared in the fragment to +184 bp. It appeared again at +338 bp but disappeared again to +609 bp. The fragment from +99 to +184 inhibited CAT activity by a post-transcriptional mechanism, as RNA of CAT was observed but CAT activity was not.
Takimoto
Y
Y
Department of Internal Medicine, Hiroshima University, Japan.
Kuramoto
A
A
D001483:Base Sequence; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D012313:RNA / Q000032:analysis; D014158:Transcription, Genetic; D014162:Transfection
D010982:Platelet-Derived Growth Factor; D012313:RNA; D015500:Chloramphenicol O-Acetyltransferase