title: Proteolysis of insulin-like growth factor binding protein-5 by pregnancy serum and amniotic fluid.
abstract: Incubation of iodinated recombinant human insulin-like growth factor binding protein (rhIGFBP)-5 with pregnancy serum or amniotic fluid resulted in the formation of 22- and 15 kDa fragments. Non-pregnancy serum did not contain IGFBP-5 proteolytic activity. Size fractionation revealed the proteolytic activity both in serum and amniotic fluid in a > 100 kDa fraction which co-eluted in gel filtration with proteins of approx. 200 kDa. The IGFBP-5 protease activity was inhibited by EDTA, phenanthroline and PMSF. The formation of proteolytic fragments was also observed using 125I labeled rhIGFBP-3 and -4 but not with rhIGFBP-1 or -6 as substrate. The data demonstrate that pregnancy serum and amniotic fluid contain a very similar cation-dependent serine protease which degrades IGFBP-3, -4 and -5.
authors.affiliation: Institute for Biochemistry II, University of Göttingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Claussen
authors.forename: J
authors.initials: J
authors.lastname: Zapf
authors.forename: T
authors.initials: T
authors.lastname: Braulke
mesh_terms: D000653:Amniotic Fluid / Q000378:metabolism*; D002352:Carrier Proteins / Q000097:blood* / Q000378:metabolism*; D005260:Female; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins; D010447:Peptide Hydrolases / Q000378:metabolism*; D011247:Pregnancy / Q000378:metabolism*; D013002:Somatomedins / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D018969:Insulin-Like Growth Factor Binding Proteins; D013002:Somatomedins; D010447:Peptide Hydrolases