1C5 antigen: cancer-associated protein expressed by cervical adenocarcinomas and beta-casein.
The monoclonal antibody 1C5 reacts with an antigenic determinant present in 90% of the cases of adenocarcinoma of the uterine cervix. The antigen defined by the 1C5 antibody exhibits immunological characteristics similar to those of skim milk (bovine buttermilk). 1C5-defined antigen obtained from tumor extract and skim milk binds specifically to wheat germ agglutinin (WGA) lectin. The 1C5-defined antigenic activity of a WGA lectin-bound fraction was eluted at 0.7-0.8 M NaCl off a Mono Q column. Use of an inhibition assay and a dot immunobinding assay revealed that the antigenic epitope defined by the 1C5 MoAb from skim milk exists within the first 28 amino acids of the beta-casein peptide.
Horimoto
E
E
Department of Obstetrics and Gynecology, Sapporo Medical University School of Medicine, Japan.
Uede
T
T
Koizumi
M
M
Kikuchi
K
K
Kudo
R
R
D000230:Adenocarcinoma / Q000276:immunology*; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology; D002364:Caseins / Q000737:chemistry*; D000939:Epitopes / Q000032:analysis*; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D002364:Caseins; D000939:Epitopes