Isolation of peritoneal precursors of B-1 cells in the adult mouse.
Two weeks of daily peritoneopheresis of adult mice result in the selective depletion of B-1 cells, followed by the appearance of a population of B220+IgM-lymphocytes in the peritoneal cavity. These cells share with bone marrow (BM) pre-B cells expression of lambda 5, VpreB, and RAG-1 genes and a higher fraction of unrearranged V to DJ heavy (H) chain immunoglobulin (Ig) gene segments, when compared with mature B lymphocytes. Upon transfer to SCID recipients, sorted peritoneal B220+IgM- cells fail to colonize the BM, repopulate very few B cells in the spleen, but entirely reconstitute the B-1 cell compartment in the peritoneal and pleuropericardial cavities. In contrast, parallel transfers of sorted BM and pleuropericardial cavities. In contrast, parallel transfers of sorted BM B220+IgM- cells result in reconstitution of the BM and spleen B lineage cell compartments, but in no coelomic B cell repopulation. Both types of pre-B cells reconstitute splenic plasma cells of donor origin, but with markedly distinct efficiencies: the ratio of IgM-plasma cell/B cell numbers in the spleens of peritoneal pre-B cell recipients is more than 500-fold higher than that of recipients reconstituted by BM pre-B cells. We take these data to indicate that (1) differentiative commitment to the B-1 cell population occurs before selection events on mature cells; (2) B-1 precursors exist or may be locally produced in the adult mouse; (3) there is a lineage-related differential ability of mature B cells to undergo terminal differentiation to high-rate Ig secretion.
Marcos
M A
MA
Unité d'Immunobiologie, CNRS URA 359, Institut Pasteur, Paris.
Gaspar
M L
ML
Malenchere
E
E
Coutinho
A
A
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D016175:B-Lymphocyte Subsets / Q000637:transplantation; D001483:Base Sequence; D002469:Cell Separation; D005434:Flow Cytometry; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D007075:Immunoglobulin M / Q000032:analysis; D007937:Leukapheresis / Q000379:methods*; D017493:Leukocyte Common Antigens; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D016513:Mice, SCID; D008969:Molecular Sequence Data; D010533:Peritoneal Lavage; D016133:Polymerase Chain Reaction; D012313:RNA / Q000302:isolation & purification
D000954:Antigens, Surface; D007075:Immunoglobulin M; D012313:RNA; D017493:Leukocyte Common Antigens