Design and fabrication of biodegradable polymer devices to engineer tubular tissues.
Engineering new tissues by transplanting cells on polymeric delivery devices is one approach to alleviate the vast shortage of donor tissue. However, it will be necessary to fabricate cell delivery devices that deliver cells to a given location and promote the formation of specific tissue structures from the transplanted cells and the host tissue. This report describes the design and fabrication of a polymeric device for guiding the development of tubular vascularized tissues, which may be useful for engineering a variety of tissues including intestine, blood vessels, tracheas, and ureters. Porous films of poly (D, L-lactic-co-glycolic acid) have been formed and fabricated into tubes capable of resisting compressional forces in vitro and in vivo. These devices promote the ingrowth of fibrovascular tissue following implantation into recipient animals, resulting in a vascularized, tubular tissue. To investigate the utility of these devices as cell delivery devices, enterocytes (intestinal epithelial cells) were seeded onto the devices in vitro. Enterocytes were found to attach to these devices and form an organized epithelial cell layer. These results suggest that these devices may be an appropriate delivery vehicle for transplanting cells and engineering new tubular tissues.
Mooney
D J
DJ
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139.
Organ
G
G
Vacanti
J P
JP
Langer
R
R
D000818:Animals; D001704:Biopolymers; D004848:Epithelium / Q000637:transplantation; D007422:Intestines / Q000637:transplantation*; D019344:Lactic Acid; D008855:Microscopy, Electron, Scanning; D009389:Neovascularization, Pathologic; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011108:Polymers; D051381:Rats; D011917:Rats, Inbred Lew; D013314:Stress, Mechanical; D013997:Time Factors; D014180:Transplantation / Q000379:methods*; D014185:Transplantation, Isogeneic / Q000379:methods*
D001704:Biopolymers; D011108:Polymers; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid