title: Evaluation of antibody against AR142, a synthetic peptide derived from the core sequence of hepatitis C virus, in the diagnosis of non-A, non-B chronic liver disease.
abstract: We evaluated serum anti-hepatitis C virus (HCV) using a synthetic peptide (AR142) which includes an epitope in the core region of HCV. The incidence of anti-AR142 in 98 patients with type non-A, non-B chronic liver diseases (NANB-CLD) was 89.8%, while all the 28 patients with non-type C chronic liver diseases were negative for anti-AR142. Among 98 NANB-CLD patients, 74 were positive for both anti-AR142 and anti-C100-3, 23 showed discordant results, and one was positive for neither. Eighty-one NANB-CLD patients underwent reverse transcription-polymerase chain reaction assay to detect viremia and 76 (93.8%) had a detectable level of HCV-RNA. Titers of anti-AR142 were not different among groups of different disease activities, genotypes of HCV, nor amount of serum HCV-RNA. These observations suggest that anti-AR142 could be a useful marker for chronic HCV infection.
authors.affiliation: First Department of Internal Medicine, Okayama University Medical School.
authors.forename: L
authors.initials: L
authors.lastname: Du
authors.forename: H
authors.initials: H
authors.lastname: Shimomura
authors.forename: H
authors.initials: H
authors.lastname: Nakagawa
authors.forename: T
authors.initials: T
authors.lastname: Hasui
authors.forename: H
authors.initials: H
authors.lastname: Tsuji
authors.forename: M
authors.initials: M
authors.lastname: Wato
authors.forename: J
authors.initials: J
authors.lastname: Kondo
authors.forename: M
authors.initials: M
authors.lastname: Ukida
authors.forename: G
authors.initials: G
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics; D005260:Female; D006508:Hepatitis Antibodies / Q000097:blood*; D006526:Hepatitis C / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D018937:Hepatitis C Antibodies; D018936:Hepatitis C Antigens; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology; D012367:RNA, Viral / Q000097:blood; D014758:Viral Core Proteins / Q000235:genetics / Q000276:immunology*; D014766:Viremia / Q000175:diagnosis / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D006508:Hepatitis Antibodies; D018937:Hepatitis C Antibodies; D018936:Hepatitis C Antigens; D010455:Peptides; D012367:RNA, Viral; D014758:Viral Core Proteins