title: Phylogenic homogeneity of Coxiella burnetii strains as determinated by 16S ribosomal RNA sequencing.
abstract: DNA coding for the 16S rRNA of six strains of the obligate intracellular bacterium Coxiella burnetii was directly amplified from lysed host cells using the polymerase chain reaction. The amplification product was sequenced using a linear-PCR procedure and compared with other published 16S rRNA sequences. The results of this analysis confirm the position of C. burnetii in the gamma subgroup of the proteobacteria. The data show that all of the C. burnetii strains are highly related (> 99%) on the basis of 16S rRNA sequences although they had different geographic origins and phenotypic characteristics. The data support a phylogenetic homogeneity of the genus Coxiella with only one species which is C. burnetii.
authors.affiliation: Faculté de Médecine, Unité des Rickettsies, Marseille, France.
authors.forename: A
authors.initials: A
authors.lastname: Stein
authors.forename: N A
authors.initials: NA
authors.lastname: Saunders
authors.forename: A G
authors.initials: AG
authors.lastname: Taylor
authors.forename: D
authors.initials: D
authors.lastname: Raoult
mesh_terms: D001483:Base Sequence; D016997:Coxiella burnetii / Q000235:genetics*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000737:chemistry; D005843:Geography; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S