Detection of Aeromonas salmonicida, causal agent of furunculosis in salmonid fish, from the tank effluent of hatchery-reared Atlantic salmon smolts.
The fish pathogen, Aeromonas salmonicida, could be detected only by bacteriological culture from the kidney of dead or moribund fish in one tank in a hatchery rearing Atlantic salmon (Salmo salar L.) smolts. However, by using a DNA probe specific for this species, allied to a PCR assay, the pathogen could be detected in water, feces and effluent samples taken from this fish tank. Also, the presence of the pathogen was found in effluent samples from two fish tanks containing apparently healthy fish. Subsequently, the presence of pathogen in these tanks was confirmed by an increase in the daily mortality rate and by a plate culture from moribund fish.
O'Brien
D
D
Recombinant DNA Group, University College Galway, Ireland.
Mooney
J
J
Ryan
D
D
Powell
E
E
Hiney
M
M
Smith
P R
PR
Powell
R
R
D000333:Aeromonas / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D000818:Animals; D001483:Base Sequence; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005393:Fish Diseases / Q000175:diagnosis / Q000209:etiology / Q000382:microbiology; D005667:Furunculosis / Q000209:etiology / Q000382:microbiology / Q000662:veterinary; D016905:Gram-Negative Bacterial Infections / Q000209:etiology / Q000382:microbiology / Q000662:veterinary; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012474:Salmon / Q000382:microbiology*
D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S