Association of RNA with human papillomavirus E7 protein of type 16 but not type 6b.
Nonfused human papillomavirus (HPV) type 16 and 6b E7 proteins were expressed in E. coli and fractionated by ion exchange and gel filtration chromatography. The E7 protein of type 6b was purified, but that of type 16 was found to be associated with RNA molecules which could not be excluded by repeated chromatography. The type 16 E7 protein in CaSki cells was also associated with RNA molecules of the same size.
Chinami
M
M
Department of Virology, Kurume University School of Medicine, Japan.
Moriyama
K
K
Fukumaki
Y
Y
Terada
M
M
Shingu
M
M
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002460:Cell Line; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004926:Escherichia coli / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G; D051379:Mice / Q000276:immunology; D009856:Oncogene Proteins, Viral / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D027383:Papillomaviridae / Q000378:metabolism*; D050725:Papillomavirus E7 Proteins; D011485:Protein Binding; D012329:RNA, Bacterial / Q000302:isolation & purification / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012329:RNA, Bacterial; C059731:oncogene protein E7, Human papillomavirus type 16