Z-RNA. The synthesis of 2'-O-[13C]methyl- and 5-methyl-analogs of ribo-CGCGCG.
Chemical synthesis of 2'-O-[13C]methyl-rCGCGCG and 5-methyl-rCGCGCG using support-aided phosphoramidite method is presented. 2'-O-Methyl guanosine derivative was separated from its 3'-O-methyl counterpart using transient 5',3'-O-silylation with 1,3-dichloro-1,1,3,3-tetraisopropyldisiloxane (Markiewicz reagent). The hexamers were obtained in a purity suitable for NMR studies.
Biała
E
E
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.
Milecki
J
J
Kowalewski
A
A
Popenda
M
M
Antkowiak
W Z
WZ
Adamiak
R W
RW
D001483:Base Sequence; D002247:Carbon Isotopes; D008722:Methods; D008745:Methylation; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
D002247:Carbon Isotopes; D009843:Oligoribonucleotides; D012313:RNA