Pseudomonas flavescens sp. nov., isolated from walnut blight cankers.
Two pseudomonad strains that produce a yellow cellular pigment, in addition to a diffusible fluorescent pigment on Kings medium B, were isolated from cankers on walnut trees. Biochemical properties, such as a positive oxidase reaction, a negative arginine dihydrolase reaction, and the production of a fluorescent pigment, in addition to the results of an extensive nutritional characterization study and DNA-DNA hybridization experiments, indicated that these strains belong to a new Pseudomonas rRNA group I species. This conclusion was supported by the results of a determination of the sequence of the PCR-amplified 16S rRNA gene and a comparison with the 16S rRNA genes of other bacterial species. The genomic DNAs of the strains had a base composition of 63 mol% G+C. The name Pseudomonas flavescens sp. nov. is proposed. Strain B62 (= NCPPB 3063) is the type strain of the species.
Hildebrand
D C
DC
Department of Plant Pathology, University of California, Berkeley 94720.
Palleroni
N J
NJ
Hendson
M
M
Toth
J
J
Johnson
J L
JL
D004269:DNA, Bacterial / Q000235:genetics; D005407:Flagella / Q000648:ultrastructure; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D010860:Pigments, Biological / Q000032:analysis; D011549:Pseudomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D009626:Terminology as Topic; D014197:Trees / Q000382:microbiology*
D004269:DNA, Bacterial; D010860:Pigments, Biological; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S