Absence of bcr/abl gene in single hemopoietic progenitors in some patients with chronic myelogenous leukemia.
CD34+DR- and CD34+DR+ cells were isolated from the marrow mononuclear cells of five patients with chronic myelogenous leukemia (CML) carrying the Philadelphia (Ph) chromosome. Analysis of bcr/abl hybrid mRNA in individual colonies from a single cell using reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated the presence or absence of the hybrid mRNA. For patient 1 in the chronic phase of CML, the hybrid mRNA was detected in all colonies derived from CD34+DR+ and CD34+DR- hemopoietic progenitors. In contrast, for patient 2 in the chronic phase of CML, the mRNA was detected in all individual colonies from CD34+DR+ progenitors but not in any from CD34+DR- progenitors. For patient 3 in the chronic phase of CML, the mRNA was detected in all individual colonies from CD34+DR+ but in only some of the colonies from CD34+DR- progenitors. For patients 4 and 5 in the acute crisis of CML, the mRNA was found in a portion of colonies from CD34+DR+ and CD34+DR- progenitors. These results indicated that normal clones can persist in CD34+DR- progenitors in some patients with CML, even when chromosome analysis detects the Ph chromosome in all metaphases of bone marrow cells.
Misawa
M
M
Department of Blood Transfusion, Hyogo College of Medicine, Japan.
Maeda
H
H
Hara
H
H
Yamamoto
Y
Y
Furuyama
J
J
D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D000951:Antigens, Neoplasm / Q000032:analysis; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis; D001752:Blast Crisis / Q000235:genetics* / Q000473:pathology; D001853:Bone Marrow / Q000473:pathology; D002648:Child; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000032:analysis / Q000235:genetics*; D016313:Genes, abl; D006684:HLA-DR Antigens / Q000032:analysis; D006412:Hematopoietic Stem Cells / Q000737:chemistry*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000473:pathology; D015466:Leukemia, Myeloid, Chronic-Phase / Q000235:genetics* / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells / Q000737:chemistry*; D009857:Oncogenes; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D012194:RNA-Directed DNA Polymerase
D015703:Antigens, CD; D018952:Antigens, CD34; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D006684:HLA-DR Antigens; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D016044:Fusion Proteins, bcr-abl; D012194:RNA-Directed DNA Polymerase