title: Transcription regulation of ribosomal protein genes at different growth rates in continuous cultures of Kluyveromyces yeasts.
abstract: We have investigated the relationship between the growth rate of two Kluyveromyces strains that differ in their maximum growth rate, namely K. lactis (mumax = 0.5 h-1) and K. marxianus (mumax = 1.1 h-1), and the transcription rate of ribosomal protein (rp) genes in these strains. The growth rate of either strain was varied by culturing the cells in a chemostat under conditions of glucose limitation at different dilution rates. Although the steady-state levels of transcription of the rp-genes of both Kluyveromyces strains were tightly coupled to the cellular growth rate, no clear relationship between the level of rp-gene transcription and the amount of in vitro binding of the RAP1- and ABF1-like proteins to the promoters of these rp-genes was observed. Upon a sudden increase in the growth rate of a steady-state culture, the transcription of rp-genes of K. lactis showed a different response from that in K. marxianus. Whereas a substantial overexpression of the K. lactis rp-genes was found during at least 4-5 h, the level of expression of the K. marxianus rp-genes was almost immediately adjusted to the new growth rate.
authors.affiliation: Department of Biochemistry and Molecular Biology, Vrije Universiteit Amsterdam, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Hoekstra
authors.forename: P
authors.initials: P
authors.lastname: Groeneveld
authors.forename: H W
authors.initials: HW
authors.lastname: Van Verseveld
authors.forename: A H
authors.initials: AH
authors.lastname: Stouthamer
authors.forename: R J
authors.initials: RJ
authors.lastname: Planta
mesh_terms: D015152:Blotting, Northern; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D005809:Genes, Regulator; D007716:Kluyveromyces / Q000235:genetics* / Q000254:growth & development; D010101:Oxygen Consumption; D012313:RNA / Q000032:analysis; D012097:Repressor Proteins / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D034501:Telomere-Binding Proteins; D014157:Transcription Factors
chemical_list: C061886:ABF1 protein, S cerevisiae; D015342:DNA Probes; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D012097:Repressor Proteins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D034501:Telomere-Binding Proteins; D014157:Transcription Factors; C074461:RIF1 protein, S cerevisiae; D012313:RNA