Screening anti-HIV Chinese materia medica with HIV and equine infectious anemic virus reverse transcriptase.
The inhibitory activities of human immunodeficiency virus reverse transcriptase (HIV-RT) inhibitors PFA and Suramin against HIV-RT and equine infectious anemic virus reverse transcriptase (EIAV-RT) were studied in this paper. The 50% inhibitory concentration (IC50) of HIV-RT and EIAV-RT treated by PFA and Suramin were 0.2 mumol, 9.8 mumol and 17 mumol, 19.9 mumol, respectively. More than thirty Chinese medicines, including recipes, herbs, extracts of traditional materia medica and isolated compounds were tested using HIV-RT and EIAV-RT as target enzymes. It was found that 5 crude extracts such as Rhizoma Polygoni Cuspidati, and Radix Notoginseng, 7 isolated compounds like Flavone of Ramulus Visci, Flavone of Ajuga Decumbens Thumb, and Aristolochic acid, as well as the extract of the complex prescription Xiao Chai Hu Tang have shown various inhibitory actions on these two enzymes. The activities of the two enzymes were comparable, but HIV-RT was more sensitive, suggesting that HIV-RT can be used as index for the screening of anti-aids drugs.
Tang
X
X
Institute of Medical Biotechnology, CAMS, Beijing.
Chen
H
H
Zhang
X
X
Quan
K
K
Sun
M
M
D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006678:HIV / Q000187:drug effects* / Q000201:enzymology; D054303:HIV Reverse Transcriptase; D004860:Infectious Anemia Virus, Equine / Q000187:drug effects* / Q000201:enzymology; D012194:RNA-Directed DNA Polymerase / Q000187:drug effects* / Q000378:metabolism; D013498:Suramin
D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D013498:Suramin; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase