Rat contrapsins are the type II acute phase proteins: regulation by interleukin 6 on the mRNA level.
Three highly homologous serine protease inhibitors, SPI-1, SPI-2 and SPI-3 (contrapsins), are synthesized in rat liver. Their expression is regulated differently in healthy and inflamed animals. We found that interleukin 6 (IL-6), a major acute phase cytokine, and to a lesser extent leukemia inhibitory factor (LIF), both together with glucocorticoids, are responsible for the regulation of expression of the contrapsins in rat hepatocytes in primary culture. The effect of IL-6 is time- and dose-dependent. IL-1, TGF beta 1, HGF, PMA and IL-8 did not have any effect on contrapsin mRNA levels. We postulate that SPI-1, SPI-2 and SPI-3 belong to the class II acute phase proteins. Additionally, we show induction of SPI-3 mRNA in rat liver by in situ hybridization using a specific oligonucleotide probe.
Kordula
T
T
Jagiellonian University, Institute of Molecular Biology, Krakow, Poland.
Bugno
M
M
Lason
W
W
Przewlocki
R
R
Koj
A
A
D000209:Acute-Phase Proteins / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D016207:Cytokines / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D017403:In Situ Hybridization; D015850:Interleukin-6 / Q000494:pharmacology*; D008099:Liver / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D015843:Serpins; D014361:Trypsin Inhibitors / Q000235:genetics*
D000209:Acute-Phase Proteins; D016207:Cytokines; D018076:DNA, Complementary; D015850:Interleukin-6; D009838:Oligodeoxyribonucleotides; D015843:Serpins; D014361:Trypsin Inhibitors; C033691:contrapsin