[Glutathione S-transferases in carcinogenesis and diagnosis of liver cancer].
We studied the relations between glutathione S-transferases (GSTs) from human liver and hepatic cancer and preneoplastic lesions in high risk area of Qidong city and Beijing. A biotin-avidin enzyme-linked immunoassay (BA-ELISA) for serum level of GSTs was developed. The results showed that the GSTs level of normal controls in Qidong city (0.66 +/- 0.54 ng/ml) was higher than that in Beijing (0.37 +/- 0.27 ng/ml). The members of high cancer families, HBVsAg carries and patients having a low level fluctuating pattern of serum AFP were the population with high risk of liver cancer; their serum level of GSTs was 1.25 +/- 1.46, 1.43 +/- 1.44 and 2.81 +/- 1.76 ng/ml respectively, and it was significantly higher than in the normal controls. The content of GSTs of hepatic cancer patients in Qidong city and Beijing was 3.03 +/- 3.35 and 3.60 +/- 3.70 ng/ml respectively, but the positive rate (97%) of GSTs in Qidong city was higher than that (82%) in Beijing. The results suggested that serum expression of GSTs can be used as an enzyme marker for hepatic cancer and preneoplastic lesions. Possible roles of these forms in hepatic carcinogenesis induced by chemical carcinogenesis were discussed.
Li
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Institute of Basic Medical Sciences of PLA, Beijing.
D014408:Biomarkers, Tumor / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005982:Glutathione Transferase / Q000097:blood*; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000209:etiology; D008297:Male; D000509:alpha-Fetoproteins / Q000378:metabolism
D014408:Biomarkers, Tumor; D007527:Isoenzymes; D000509:alpha-Fetoproteins; D005982:Glutathione Transferase