Isolation and sequence analysis of peptides from the venom of Protonectarina sylveirae (Hymenoptera-Vespidae).
Three new venom peptides were isolated from the Brazilian wasp; Protonectarina sylveirae and their complete amino acid sequences were determined by Edman degradation as well as by FAB-mass spectrometry. One (P-8) of them was a new analog of mastoparan family; therefore, we named it Protonectarina mastoparan. Another peptide (P-10) had an amino acid sequence homology with Ves-CP-T, a chemotactic peptide of wasp venom, and bombolitin-V, the venom peptide from bumblebee. The third peptide (P-6) was structurally unique, possessing an intramolecular disulfide bond. Not only Protonectarina mastoparan (P-8) but also P-6 and P-10 caused histamine release from rat peritoneal mast cells as potently as mastoparan (EC50s were about 1 x 10(-6) M for P-6 and 2 x 10(-6) M for P-10). In addition, P-10 in a concentration higher than 1 x 10(-5) M induced hemolysis though whose hemolytic activity was about a half potency of that of mastoparan. However, P-6 did not cause hemolysis up to the concentration of 10 microM. We have named them sylverin for the peptide P-6 and protonectin for the peptide P-10.
Dohtsu
K
K
Research Center, Daicel Chemical Industries Ltd., Himeji, Japan.
Okumura
K
K
Hagiwara
K
K
Palma
M S
MS
Nakajima
T
T
D000595:Amino Acid Sequence; D000818:Animals; D019204:GTP-Binding Proteins / Q000502:physiology; D006636:Histamine Release / Q000187:drug effects; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D014862:Wasp Venoms / Q000032:analysis*; D014863:Wasps
D010455:Peptides; D014862:Wasp Venoms; D019204:GTP-Binding Proteins