Structural comparison of the O4-specific polysaccharides from E. coli O4:K6 and E. coli O4:K52.
Two distinct forms of the O4 antigen (LPS) from E. coli were analysed by 1H and 13C NMR spectroscopy. Both consisted of D-glucose, L-rhamnose, 2-acetamido-2,6-dideoxy-L-galactose (L-FucNAc), and 2-acetamido-2-deoxy-D-glucose. Their structures were found to be [formula: see text]. In the O4-specific polysaccharide from E. coli O4:K3, O4:K6, and O4:K12, X is alpha-D-Glcp. In the O4 specific polysaccharide from E. coli O4:K52, the rhamnose residue is not substituted (X = H).
Jann
B
B
Max-Planck-Institut für Immunobiologie, Freiburg, Germany.
Shashkov
A S
AS
Kochanowski
H
H
Jann
K
K
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D004926:Escherichia coli / Q000737:chemistry* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D013045:Species Specificity
D002241:Carbohydrates; D019081:O Antigens; D011135:Polysaccharides, Bacterial