Identification of differentially expressed mRNAs during neuronal differentiation of P19 embryonal carcinoma cells.
To understand the basic mechanisms underlying neuronal differentiation, we have attempted to isolate differentially expressed genes, which may play a key role in this complex process, from neuronal differentiating P19 embryonal carcinoma cells. RNA fingerprinting by the arbitrarily primed PCR (RAP) method was adapted to detect such differentially expressed genes during P19 neuronal differentiation. Using this method with some modifications, we successfully cloned seven cDNA fragments which were expressed differentially within the first 48 h after 1 microM retinoic acid (RA) treatment, which ultimately induces neuronal differentiation. Comparison of the partial nucleotide sequences of these clones with sequences in DNA databases indicated that one of these clones was identical to a region of the mouse Oct-3 gene, which has been shown to be dramatically repressed by RA. Two clones were highly homologous to the human profilinII and leucine-rich protein genes. The other four clones were not closely related to any sequences in the databases. Except for the Oct-3 gene, the other six genes isolated here have not been reported previously as RA-regulated genes. RAP is, thus, a promising method for identification of novel and potentially important genes which are differentially regulated during neuronal differentiation.
Suzuki
Y
Y
Department of Molecular Neurobiology (Tanabe), Osaka University Medical School, Japan.
Wanaka
A
A
Tohyama
M
M
Takagi
T
T
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000378:metabolism; D009710:Nucleotide Mapping; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D012333:RNA, Messenger