The groE gene products of Escherichia coli are dispensable for mucA+B(+)-dependent UV mutagenesis.
UV mutagenesis in Escherichia coli requires the groES+EL+ chaperonins as well as the umuD+C+ SOS-regulated genes. GroES and GroEL appear to be required to stabilize UmuC. The mucA+B+ genes, which are encoded on a broad host range plasmid, are functionally analogous and structurally similar to the umuD+C+ genes of E. coli. While these gene pairs are quite similar, differences have been reported in the functioning of these gene products. We tested whether mucA+B+ function requires the groE+ gene products as well. We show that mucA+B(+)-induced UV mutagenesis, UV resistance, phage reactivation and cold sensitivity do not require the groE+ heat shock genes. These findings suggest that the requirement of UmuC for groES+EL+ function is not shared by its analog, MucB.
Donnelly
C E
CE
Department of Biology 56-621, Massachusetts Institute of Technology, Cambridge 02139.
Murli
S
S
Walker
G C
GC
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001435:Bacteriophages / Q000235:genetics / Q000528:radiation effects; D018835:Chaperonin 10; D018834:Chaperonin 60; D003080:Cold Temperature; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D005798:Genes, Bacterial; D005838:Genotype; D006360:Heat-Shock Proteins / Q000378:metabolism*; D016296:Mutagenesis; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D018835:Chaperonin 10; D018834:Chaperonin 60; D006360:Heat-Shock Proteins; C063757:MucA protein, Bacteria