Productive re-arrangement at both alleles of the T-cell receptor beta-chain locus in CD4 T-cell clones specific for influenza haemagglutinin.
T-cell receptor (TcR) beta-chain usage, and VDJ junctional region sequences thereof in a panel of CD4+ T-cell clones Ad-, or Ak- or Ek-restricted for major antigenic sites of influenza haemagglutinin (H3 subtype), were investigated. Direct sequencing of cDNA, obtained by polymerase chain reaction, revealed that the majority of T-cell clones contained both productive and non-productive rearranged transcripts. Moreover, T-cell clones specific for p206-227 (Ad) or p245-265 (AK) contained double-productive re-arranged transcripts (V beta 6 J beta 2.6, V beta 4, J beta 1.2) and (V beta 6 J beta 1.3, V beta 8.2, J beta 1.5) respectively. However, FACS analysis with V beta-specific monoclonal antibodies established that, for each of these T-cell clones, only a single beta-chain was expressed at the cell surface, thereby indicating post-transcriptional editing.
Smith
C A
CA
National Institute for Medical Research, London, U.K.
Graham
C M
CM
Thomas
D B
DB
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D004279:DNA, Viral / Q000737:chemistry; D000939:Epitopes / Q000276:immunology; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / Q000276:immunology*; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis
D004279:DNA, Viral; D000939:Epitopes; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D016693:Receptors, Antigen, T-Cell, alpha-beta