Retinoblastoma protein monoclonal antibodies with novel characteristics.
We have developed a family of monoclonal antibodies directed against the retinoblastoma gene product (p110RB). One of these monoclonal antibodies, 3C8, binds p110RB near the C-terminal end of the protein (aa886-aa905). It was characterized by immunoblotting, ELISA, fluorescence-activated flow cytometry and immunohistostaining. It was shown to be useful for the detection of p110RB in formalin-fixed and paraffin-embedded tissue sections. Because 3C8 binds outside of regions shown to be involved in p110RB interactions with other cellular proteins, it may be an especially useful reagent for the reliable detection of p110RB in tumor cells, and for the isolation by affinity chromatography of p110RB complexes with other cellular proteins.
Wen
S F
SF
Department of Assay Development, Canji Inc., San Diego, CA 92121.
Nodelman
M
M
Nared-Hood
K
K
Duncan
J
J
Geradts
J
J
Shepard
H M
HM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D014408:Biomarkers, Tumor; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D016161:Genes, Retinoblastoma / Q000276:immunology; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D012175:Retinoblastoma / Q000175:diagnosis; D016160:Retinoblastoma Protein / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D000939:Epitopes; D016160:Retinoblastoma Protein