Small cytoplasmic RNA of Bacillus brevis: transcriptional and phylogenetic analysis.
Using a DNA fragment of Bacillus subtilis scRNA as a probe, a Bacillus brevis gene encoding the small cytoplasmic RNA was cloned and characterized. B. brevis scRNA consists of 273 nucleotides; the sequence has comparatively low homology (approximately 70%) with other Bacillus sequences. Phylogenetic analysis indicated that B. brevis forms a line of descent distinct from other Bacillus species. However, despite the low overall homology, both functional nucleotide sequence and secondary structural features defined among signal recognition particle (SRP) RNA family members were well conserved.
Nakamura
K
K
Institute of Biological Sciences, University of Tsukuba, Ibarak, Japan.
Nishiguchi
M
M
Minemura
M
M
Honda
K
K
Yamane
K
K
D001407:Bacillus / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012329:RNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D013045:Species Specificity; D014158:Transcription, Genetic
D017931:DNA Primers; D015342:DNA Probes; D012329:RNA, Bacterial