Identification of a peptide recognized by five melanoma-specific human cytotoxic T cell lines.
Of several thousand peptides presented by the major histocompatibility molecule HLA-A2.1, at least nine are recognized by melanoma-specific cytotoxic T lymphocytes (CTLs). Tandem mass spectrometry was used to identify and to sequence one of these peptide epitopes. Melanoma-specific CTLs had an exceptionally high affinity for this nine-residue peptide, which reconstituted an epitope for CTL lines from each of five different melanoma patients tested. Recognition by multiple CTL lines suggests that this may be a promising candidate for use in peptide-based melanoma vaccines.
Cox
A L
AL
Department of Chemistry, University of Virginia, Charlottesville 22908.
Skipper
J
J
Chen
Y
Y
Henderson
R A
RA
Darrow
T L
TL
Shabanowitz
J
J
Engelhard
V H
VH
Hunt
D F
DF
Slingluff
C L
CL
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D000939:Epitopes / Q000276:immunology; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D013058:Mass Spectrometry; D008545:Melanoma / Q000276:immunology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D000939:Epitopes; D015789:HLA-A2 Antigen; D009842:Oligopeptides; C086976:peptide 946