Binding of native and [homoserine lactone-52]-52,53-seco-bovine basic pancreatic trypsin inhibitor (Kunitz inhibitor) to porcine pancreatic beta-kallikrein-B and bovine alpha-chymotrypsin: thermodynamic study.
Values of the association equilibrium constant (Ka) for the binding of the native and of the cyanogen bromide-cleaved bovine basic pancreatic trypsin inhibitor (native BPTI and [Hse lactone-52]-52,53-seco-BPTI, respectively) to neuraminidase-treated porcine pancreatic beta-kallikrein-B (kallikrein) and bovine alpha-chymotrypsin (chymotrypsin) have been determined between pH 4.0 and 9.0, at 20.0 degrees C. Over the whole pH range explored, native BPTI and [Hse lactone-52]-52,53-seco-BPTI show the same affinity for kallikrein. On the other hand, the affinity of [Hse lactone-52]-52,53-seco-BPTI for chymotrypsin is higher, around neutrality, than that found for native BPTI by about one order of magnitude, converging in the acidic pH limb. The simplest mechanism accounting for the observed data implies that, on lowering the pH from 9.0 to 4.0, (i) the decrease in affinity for the binding of native BPTI to kallikrein and chymotrypsin, as well as for the association of [Hse lactone-52]-52,53-seco-BPTI to kallikrein, reflects the acidic pK shift, upon inhibitor association, of a single ionizing group; and (ii) the decrease of Ka values for [Hse lactone-52]-52,53-seco-BPTI binding to chymotrypsin appears to be modulated by the acidic pK shift, upon inhibitor association, of two non-equivalent proton-binding residues. On the basis of the stereochemistry of the serine proteinase/inhibitor contact region(s), these data indicate that long-range structural changes in [Hse lactone-52]-52,53-seco-BPTI are energetically linked to the chymotrypsin:inhibitor complex formation. This observation represents an important aspect for the mechanism of molecular recognition and regulation in BPTI.
Oddone
R
R
CNR, Center for Molecular Biology, University of Rome La Sapienza, Italy.
Barra
D
D
Amiconi
G
G
Ascenzi
P
P
Tarricone
C
C
Bolognesi
M
M
Bortolotti
F
F
Menegatti
E
E
D000818:Animals; D007611:Aprotinin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D002918:Chymotrypsin / Q000737:chemistry / Q000378:metabolism*; D006714:Homoserine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007610:Kallikreins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008958:Models, Molecular; D009439:Neuraminidase / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D013552:Swine; D013816:Thermodynamics
C090808:(homoserine lactone-52)-52,53-seco-BPTI; D006714:Homoserine; D007611:Aprotinin; D009439:Neuraminidase; D007610:Kallikreins; D002918:Chymotrypsin