Ribosomes of Leishmania are a target for the aminoglycosides.
Ribosomes of Leishmania, a parasitic protozoan (member of the order of Kinetoplastidae), were purified on a sucrose density gradient. Two different types of ribosomes were isolated from the promastigotes: cytoplasmic (88S and 91S from L. tropica and L. donovani, respectively) and mitochondrial (75S in both species). Both types of ribosome dissociated into their subunits at low Mg2+ concentration (1-2 mM) as follows: 67S and 49S for the 91S cytoplasmic ribosomes of L. donovani and 61S and 43S for the 88S cytoplasmic ribosome of L. tropica; 55S and 34S for L. tropica and 60S and 39S for L. donovani mitochondrial ribosomes, respectively. Paromomycin (aminosidine), an aminoglycoside aminocyclitol antibiotic, interacted with the ribosomes to promote the association of the subunits. Under similar experimental conditions, spermidine and pentamidine were inactive.
Maarouf
M
M
Institut de Chimie des Substances Naturelles, C.N.R.S., Gif-Yvette, France.
Lawrence
F
F
Croft
S L
SL
Robert-Gero
M
M
D000818:Animals; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm; D005601:Framycetin / Q000494:pharmacology; D007893:Leishmania donovani / Q000187:drug effects* / Q000254:growth & development; D007895:Leishmania tropica / Q000187:drug effects* / Q000254:growth & development; D008274:Magnesium / Q000494:pharmacology; D008928:Mitochondria; D010303:Paromomycin / Q000378:metabolism / Q000494:pharmacology*; D010419:Pentamidine / Q000494:pharmacology; D012270:Ribosomes / Q000737:chemistry / Q000187:drug effects*; D013095:Spermidine / Q000494:pharmacology
D005601:Framycetin; D010303:Paromomycin; D010419:Pentamidine; D008274:Magnesium; D013095:Spermidine