title: The nad6 gene and the exon d of nad1 are co-transcribed in wheat mitochondria.
abstract: The exon d of nad1 is located 993 bp upstream of the nad6 gene in the wheat mitochondrial genome. Transcription analyses of both sequences (nad1 exon d and the nad6 gene) were done by Northern hybridization using RNA from wheat seedlings and tissue cultures derived from immature embryos. A complicated pattern was generated with a probe including exon d of nad1 and the whole nad6 gene. An 0.71-kb transcript is specific to nad1 exon d whereas a 1.2-kb transcript is specific to the nad6 gene. Three larger transcripts hybridize to both probes suggesting that nad1 exon d and nad6 are co-transcribed. This co-transcription has been directly demonstrated by cDNA synthesis on mtRNAs and sequencing of the PCR amplification product.
authors.affiliation: Laboratoire de Biologie Moléculaire Végétale associé au CNRS (UA 1128), Université Paris Sud, Orsay, France.
authors.forename: N
authors.initials: N
authors.lastname: Haouazine
authors.forename: A
authors.initials: A
authors.lastname: Takvorian
authors.forename: M F
authors.initials: MF
authors.lastname: Jubier
authors.forename: F
authors.initials: F
authors.lastname: Michel
authors.forename: B
authors.initials: B
authors.lastname: Lejeune
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D005091:Exons; D015870:Gene Expression; D017343:Genes, Plant; D007438:Introns; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000096:biosynthesis / Q000235:genetics*; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012313:RNA / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D012326:RNA Splicing; D000077278:RNA, Mitochondrial; D014158:Transcription, Genetic; D014908:Triticum / Q000201:enzymology* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D000077278:RNA, Mitochondrial; D012313:RNA; D016660:NAD(P)H Dehydrogenase (Quinone)