title: Phosphorylation of insulin-like growth factor binding protein-1 in patients with insulin-dependent diabetes mellitus and severe trauma.
abstract: We have determined the level of phosphorylated insulin-like growth factor binding protein-1 (pIGFBP-1) in serum during two catabolic states: diabetes mellitus and trauma. Human sera were incubated with [125I]IGF-I for 2 h followed by non-denaturing PAGE. [125I]IGF-I/IGFBP-1 complexes from serum co-migrated with a pure p4IGFBP-1 standard. Complex formation was specifically inhibited by unlabeled IGF-I. The migration of IGF-I/pIGFBP-1 complexes was retarded by IGFBP-1 antibodies, but not by antibodies against IGFBP-2 or IGFBP-3. Sera from three severely traumatized patients had up to 12-fold more pIGFBP-1 than sera from age-matched controls. The level of pIGFBP-1 was reduced in all three patients upon hospital discharge. Sera from three patients with insulin dependent diabetes mellitus (IDDM) and severe ketoacidosis (DKA) had more pIGFBP-1 than controls. Administration of insulin to DKA patients lowered the level of pIGFBP-1. The present study shows that IGFBP-1 exists as a free, high affinity, phosphorylated form in vivo during two catabolic states.
authors.affiliation: Department of Medicine, State University of New York at Stony Brook 11794.
authors.forename: R A
authors.initials: RA
authors.lastname: Frost
authors.forename: A
authors.initials: A
authors.lastname: Bereket
authors.forename: T A
authors.initials: TA
authors.lastname: Wilson
authors.forename: M M
authors.initials: MM
authors.lastname: Wojnar
authors.forename: C H
authors.initials: CH
authors.lastname: Lang
authors.forename: M C
authors.initials: MC
authors.lastname: Gelato
mesh_terms: D000063:Accidents, Traffic; D000293:Adolescent; D000328:Adult; D001786:Blood Glucose / Q000032:analysis; D002352:Carrier Proteins / Q000097:blood* / Q000302:isolation & purification; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D016883:Diabetic Ketoacidosis / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007328:Insulin / Q000097:blood; D018970:Insulin-Like Growth Factor Binding Protein 1; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007657:Ketone Bodies / Q000097:blood; D010766:Phosphorylation; D011485:Protein Binding; D014947:Wounds and Injuries / Q000097:blood*
chemical_list: D001786:Blood Glucose; D002352:Carrier Proteins; D007328:Insulin; D018970:Insulin-Like Growth Factor Binding Protein 1; D007657:Ketone Bodies; D007334:Insulin-Like Growth Factor I; D006854:Hydrocortisone