title: Effect of abasic sites on bacteriophage T7 protein synthesis.
abstract: We have examined protein synthesis directed by bacteriophage T7 which had been alkylated with methyl methanesulfonate so as to produce apurinic sites in its DNA in vivo. Both repair-proficient and repair-deficient (xth nfo mutant) strains of Escherichia coli served as host cells. In repair-proficient cells, all three classes of phage proteins were synthesized, although with significant delays. In mutant cells, only class I proteins were produced and their synthesis was delayed and reduced, demonstrating a perturbation of protein synthesis and providing the first in vivo indication that transcription is inhibited by abasic sites. However, the proposed effects of abasic sites on transcription appear to be weaker than those on replication.
authors.affiliation: Département de biochimie, Université de Montréal, Quebec, Canada.
authors.forename: G
authors.initials: G
authors.lastname: Sanchez
authors.forename: J F
authors.initials: JF
authors.lastname: Racine
authors.forename: M D
authors.initials: MD
authors.lastname: Mamet-Bratley
mesh_terms: D000478:Alkylation; D001080:Apurinic Acid / Q000378:metabolism*; D001345:Autoradiography; D017123:Bacteriophage T7 / Q000187:drug effects / Q000235:genetics*; D004249:DNA Damage; D004260:DNA Repair; D004279:DNA, Viral / Q000187:drug effects / Q000378:metabolism; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004706:Endodeoxyribonucleases / Q000172:deficiency; D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins; D005090:Exodeoxyribonucleases / Q000172:deficiency; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D008741:Methyl Methanesulfonate / Q000633:toxicity; D016296:Mutagenesis; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication / Q000187:drug effects
chemical_list: C041424:0.3 protein, Enterobacteria phage T7; D004279:DNA, Viral; D029968:Escherichia coli Proteins; D014764:Viral Proteins; D001080:Apurinic Acid; D008741:Methyl Methanesulfonate; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases; D004706:Endodeoxyribonucleases; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III; D043223:Deoxyribonuclease IV (Phage T4-Induced); C468972:endonuclease IV, E coli; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase