title: Effective tumor vaccine generated by fusion of hepatoma cells with activated B cells.
abstract: Fusion of BERH-2 rat hepatocellular carcinoma cells with activated B cells produced hybrid cells that lost their tumorigenicity and became immunogenic. Syngeneic rats injected with BERH-2-B hybrid cells became resistant to challenge with parental BERH-2 cells, and rats with established BERH-2 hepatomas were cured by subsequent injection of BERH-2-B cells. Both CD4+ and CD8+ cells were essential for the induction of protective immunity; however, only CD8+ cells were required for the eradication of BERH-2 tumors. The generation of hybrid tumor cells that elicit antitumor immune responses may be a useful strategy for cancer immunotherapy.
authors.affiliation: Tumor Immunology and Biotherapy Center, Eastern Institute of Hepatobiliary Surgery, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.forename: M
authors.initials: M
authors.lastname: Wu
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Liu
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Ma
authors.forename: M S
authors.initials: MS
authors.lastname: Sy
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D018122:B7-1 Antigen / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002459:Cell Fusion; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006822:Hybrid Cells / Q000276:immunology*; D016233:Immunotherapy, Active; D008114:Liver Neoplasms, Experimental / Q000276:immunology* / Q000517:prevention & control / Q000628:therapy; D008213:Lymphocyte Activation; D009368:Neoplasm Transplantation; D051381:Rats; D017208:Rats, Wistar; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014407:Tumor Cells, Cultured; D014611:Vaccination; D014612:Vaccines / Q000276:immunology*
chemical_list: D018122:B7-1 Antigen; D000949:Histocompatibility Antigens Class II; D014612:Vaccines