Immunohistochemical distribution of CD44 and desmoplakin I & II in Hailey-Hailey's disease and Darier's disease.
The cell-surface glycoprotein CD44 is found on a wide variety of cells including epidermal cells. It is involved in cell to cell adhesion. Desmoplakin I & II are important components of the attachment plaque of desmosomes. In this study, we compared the distribution patterns of anti-CD44 and anti-desmoplakin I & II in Hailey-Hailey's disease and Darier's disease. In the normal skin, anti-CD44 stained the entire periphery of epidermal keratinocytes while anti-desmoplakin I & II produced dotted staining patterns along the periphery of epidermal keratinocytes. In Hailey-Hailey's disease and Darier's disease, the staining pattern of anti-CD44 on acantholized keratinocytes did not change, but anti-desmoplakin I & II lost their peripheral, dotted patterns and stained diffusely in the cytoplasm in most acantholytic cells. These results suggest that, in Hailey-Hailey's disease and Darier's disease, CD44 may be intact even in acantholytic cells but abnormalities of desmoplakin exist in such cells.
Harada
M
M
Department of Dermatology and Syphilology, Wayne State University School of Medicine, Detroit, Michigan 48201.
Hashimoto
K
K
Fujiwara
K
K
D002352:Carrier Proteins / Q000032:analysis*; D015815:Cell Adhesion Molecules / Q000032:analysis; D003598:Cytoskeletal Proteins / Q000032:analysis*; D007644:Darier Disease / Q000276:immunology / Q000378:metabolism*; D051180:Desmoplakins; D005455:Fluorescent Antibody Technique; D006801:Humans; D018960:Hyaluronan Receptors; D016506:Pemphigus, Benign Familial / Q000276:immunology / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000032:analysis*; D016201:Receptors, Lymphocyte Homing / Q000032:analysis*; D012867:Skin / Q000737:chemistry* / Q000276:immunology
D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; C495294:DSP protein, human; D051180:Desmoplakins; D018960:Hyaluronan Receptors; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing