[Analysis of lipopolysaccharide (LPS) from impermeability-type drug resistant Pseudomonas aeruginosa using new, highly-sensitive LPS staining method].
The specific ladder pattern on polyacrylamide gel electropholesis of lipopolysaccharide (LPS) extracted from Pseudomonas aeruginosa was clearly shown by using the complex methods of the PAS staining, re-periodate oxidation and then Ag-staining method. Accordingly, it was concluded that the new method was greatly useful for a detail analysis of LPS changes in Gram-negative bacteria. And it was shown by this method that no changes in LPS occurred between the impermeability-type drug resistant P. aeruginosa mediated by R plasmid and a drug susceptible strain. The absence of changes indicated that the LPS of P. aeruginosa K-Ps102 had not role in the mechanism of the high drug resistance.
O'Hara
K
K
Department of Microbiology, School of Pharmaceutical Sciences, Tokyo College of Pharmacy.
Fukuda
H
H
Nakahara
H
H
Bryan
L E
LE
D004352:Drug Resistance, Microbial; D008070:Lipopolysaccharides / Q000032:analysis*; D010539:Permeability; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000187:drug effects; D013194:Staining and Labeling / Q000379:methods
D008070:Lipopolysaccharides