The role of ribonuclease H in multicopy single-stranded DNA synthesis in retron-Ec73 and retron-Ec107 of Escherichia coli.
Bacterial reverse transcriptase is responsible for the synthesis of multicopy single-stranded DNA (msDNA). Reverse transcriptases from retron-Ec73 and retron-Ec107 do not contain an RNase H domain. Cellular RNase H is therefore considered to be required to make the mature form of msDNA. We found that RNase HI, but not RNase HII, is required for the production of the mature form of both msDNAs.
Shimamoto
T
T
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854.
Shimada
M
M
Inouye
M
M
Inouye
S
S
D001483:Base Sequence; D004269:DNA, Bacterial / Q000096:biosynthesis*; D018076:DNA, Complementary / Q000096:biosynthesis; D004277:DNA, Single-Stranded / Q000096:biosynthesis*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D018626:Retroelements / Q000235:genetics*; D016914:Ribonuclease H / Q000378:metabolism*
D004269:DNA, Bacterial; D018076:DNA, Complementary; D004277:DNA, Single-Stranded; D018626:Retroelements; D012194:RNA-Directed DNA Polymerase; C091139:ribonuclease HII; D016914:Ribonuclease H; C078856:ribonuclease HI