title: Lymphocytes possess an electrogenic H(+)-transporting pathway in their plasma membrane.
abstract: The existence of an electrogenic H(+)-transporting pathway similar to that described in the plasma membrane of granulocytes and macrophages is reported in pig peripheral lymphocytes. The function of the H(+)-transport pathway can only be detected when free movement of charge-compensating cations is allowed. H+ transport is stimulated by arachidonic acid and various unsaturated fatty acids, and inhibited by bivalent cations, with the following sequence of efficiency: Zn2+ > Cd2+ = Co2+ = Ni2+ > Mn2+ > Ba2+ = Ca2+ = Mg2+. The transport pathway is activated by intracellular acidification and by NN'-dicyclohexylcarbodiimide, but it is not influenced by phorbol 12-myristate 13-acetate. As pig peripheral lymphocytes are not able to produce O2-., it is suggested that the operation of the electrogenic H+ conductance does not require the assembly of a functional NADPH oxidase.
authors.affiliation: Department of Physiology, Semmelweis Medical University, Budapest, Hungary.
authors.forename: K
authors.initials: K
authors.lastname: Káldi
authors.forename: K
authors.initials: K
authors.lastname: Szászi
authors.forename: K
authors.initials: K
authors.lastname: Suszták
authors.forename: A
authors.initials: A
authors.lastname: Kapus
authors.forename: E
authors.initials: E
authors.lastname: Ligeti
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D002104:Cadmium / Q000494:pharmacology; D002413:Cations, Divalent; D002462:Cell Membrane / Q000378:metabolism*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000097:blood* / Q000187:drug effects; D008214:Lymphocytes / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000097:blood; D019255:NADPH Oxidases; D011493:Protein Kinase C / Q000378:metabolism; D011522:Protons; D013552:Swine; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D002413:Cations, Divalent; D005231:Fatty Acids, Unsaturated; D007473:Ion Channels; D011522:Protons; D002104:Cadmium; D016718:Arachidonic Acid; D004024:Dicyclohexylcarbodiimide; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate