Structure of the O-specific polysaccharide of the O22-antigen (LPS) from Escherichia coli O22:K13.
The polysaccharide moiety of the O22-antigen (lipopolysaccharide, LPS) consists of 2-acetamido-2-deoxy-D-galactose, D-glucuronic acid, D-glucose, and D-galactose in the molar ratios 2:1:1:1. Methylation analysis as well as 1D and 2D NMR spectroscopy showed that the O22 polysaccharide has the primary structure [formula: see text]
Bartelt
M
M
Max-Planck-Institut für Immonbiologie, Freiburg, Germany.
Shashkov
A S
AS
Kochanowski
H
H
Jann
B
B
Jann
K
K
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry*; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial