Effects of nitric oxide-related agents on rat testicular function.
The effects of nitric oxide (NO)-related agents on testicular function were examined in male rats with measurements of serum luteinizing hormone, serum testosterone, testicular interstitial fluid (TIF) testosterone, and TIF volumes. Serum and TIF testosterone levels and luteinizing hormone secretion were significantly decreased by the NO donor, isosorbide dinitrate (ISDN), and the NO synthase (NOS) substrate, L-arginine methyl ester, a source for the endogenous production of NO. The effects of ISDN on TIF volumes were inconsistent, but L-arginine methyl ester decreased TIF formation in a dose-dependent manner. In addition, ISDN dose dependently suppressed testosterone secretion stimulated by human chorionic gonadotropin treatment, suggesting that the effects on testosterone secretion were independent of changes in secretion of the endogenous gonadotropin luteinizing hormone. ISDN, L-arginine methyl ester, and the endogenous NOS substrate L-arginine completely blocked testosterone secretion stimulated by the NOS inhibitor NG-nitro-L-arginine methyl ester (NAME), whereas the relatively inactive NOS substrate, D-arginine, only partially blocked NAME-stimulated testosterone secretion. Hydralazine and nicardipine, two vasodilators that do not exhibit prominent NO-related effects, also blocked basal testosterone secretion and testosterone secretion stimulated by the vasoconstrictor NAME.(ABSTRACT TRUNCATED AT 250 WORDS)
Adams
M L
ML
Washington University School of Medicine, Department of Psychiatry, St. Louis, Missouri.
Meyer
E R
ER
Sewing
B N
BN
Cicero
T J
TJ
D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D004347:Drug Interactions; D006830:Hydralazine / Q000494:pharmacology; D007548:Isosorbide Dinitrate / Q000494:pharmacology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester; D009529:Nicardipine / Q000494:pharmacology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000494:pharmacology* / Q000502:physiology; D019001:Nitric Oxide Synthase; D051381:Rats; D017207:Rats, Sprague-Dawley; D013256:Steroids / Q000096:biosynthesis; D013268:Stimulation, Chemical; D013737:Testis / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D013739:Testosterone / Q000097:blood / Q000378:metabolism; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology
D013256:Steroids; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; C026512:arginine methyl ester; D006830:Hydralazine; D009569:Nitric Oxide; D013739:Testosterone; D001120:Arginine; D009529:Nicardipine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D007548:Isosorbide Dinitrate; D019331:NG-Nitroarginine Methyl Ester