Observations on the contraction and movement of fat-storing cells in culture.
The Wistar fat-storing cells were isolated by perfusion with collagenase and centrifugation with metrizamide density cushion technique and cultured in vitro. The fat-storing cells were confirmed by the presentation of the lipid drop in the cytoplasm and the visualization of dismin with anti-dismin antibody by using indirect immunofluorescence method. By the videotape recorder (VIR) and the imagine analysis system, we observed the wandering immigration, the abrupt contraction of fat-storing cells with spike, then becoming a ball-like shape in its division phase. After that the cell began to extend and the contraction of these cells can be induced by the presence of 10(-2) mmol/L endothelin-1, 1 mmol/L of substance P and 2 x 10(-5) mmol/L noradrenalin. After removal of these agents the contracted cells would become extended. All these findings indicate that the fat-storing cells have ability of contraction and movement.
Wang
X E
XE
Institute of Integrated Traditional Chinese and Western Medicine, Tongji Medical University, Wuhan.
Li
M Z
MZ
Ye
W Y
WY
D017667:Adipocytes / Q000166:cytology*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D016232:Endothelins / Q000494:pharmacology*; D008099:Liver / Q000166:cytology*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013373:Substance P / Q000494:pharmacology*
D016232:Endothelins; D013373:Substance P; D009638:Norepinephrine