Evaluation of the sensitivity, specificity, and predictive value of monoclonal antibody 3F6 for the detection of Pneumocystis carinii pneumonia in bronchoalveolar lavage specimens and induced sputum.
The sensitivity and specificity of different staining procedures for the detection of Pneumocystis carinii organisms were compared. Three conventional stains (Papanicolaou, Giemsa and Grocott's) and one immunocytochemical stain using 3F6 antibody were used on smears prepared from the same specimen. Bronchoalveolar lavage (BAL) and induced sputum (IS) specimens were used for this purpose. One hundred and sixty-five episodes from 142 patients were investigated by the four different staining techniques. Cysts of P. carinii were detected in 64 episodes from 63 patients. Immunocytochemical staining with 3F6 was found to be slightly more sensitive at detecting the cysts than Grocott's, Giemsa, or Papanicolaou stain.
Wazir
J F
JF
Department of Cytopathology, Saint Mary's Hospital, London, UK.
Macrorie
S G
SG
Coleman
D V
DV
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis; D000911:Antibodies, Monoclonal; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D005069:Evaluation Studies as Topic; D006801:Humans; D007150:Immunohistochemistry; D011010:Pneumocystis / Q000302:isolation & purification; D011020:Pneumonia, Pneumocystis / Q000175:diagnosis*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology*; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal