Fibroblast responses to cytokines are maintained during aging.
Impaired wound healing in older individuals may result from a global deficit of fibroblast activity or a decreased response of aged fibroblasts to stimulation by inflammatory cytokines. Twenty-eight fibroblast cell strains were developed from normal human skin aged 3 days to 84 years. Mitogenic response to cytokines, epidermal growth factor, tumor necrosis factor-alpha, platelet-derived growth factor or fetal bovine serum was determined using a 4-hour 3H-thymidine incorporation assay. Synthesis of collagen and noncollagen proteins was determined basally and in response to transforming growth factor-beta using a 6-hour 3H-proline incorporation assay. Neither the mitogenic response to cytokine stimulation (p > 0.3) nor the synthesis of collagen and noncollagen protein after transforming growth factor-beta stimulation (p > 0.4) varied with the age of the cell donor. Individual cell lines' response to cytokine stimulation varied widely, reflecting differences commonly seen in patients' wound-healing abilities. Cellular responses to wound-healing cytokines are preserved as people age. Abnormalities in wound healing in older patients may be the result of altered immune initiation of healing or the cumulative result of concomitant disease.
Freedland
M
M
Department of Surgery, University of Michigan Medical Center, Ann Arbor.
Karmiol
S
S
Rodriguez
J
J
Normolle
D
D
Smith
D
D
Garner
W
W
D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000276:immunology*; D000818:Animals; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D003094:Collagen / Q000096:biosynthesis; D016207:Cytokines / Q000276:immunology*; D005260:Female; D005347:Fibroblasts / Q000276:immunology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D014945:Wound Healing / Q000276:immunology
D016207:Cytokines; D003094:Collagen