title: Localisation of secretory leucocyte proteinase inhibitor mRNA in nasal mucosa.
abstract: Human secretory leucocyte proteinase inhibitor (SLPI) is a low-molecular weight, acid-stable inhibitor of polymorph-nuclear granulocyte elastase and cathepsin G. Previous reports have demonstrated the existence of SLPI in the respiratory tract, salivary glands and cervical mucosa. Positive staining for SLPI using immunohistochemical techniques has been reported in serous glands in nasal mucosa. We now confirm this observation and show, using in situ hybridization, that the pattern of expression of mRNA corresponds to the distribution of the encoded protein, SLPI. This, together with the high concentration of SLPI in nasal secretions, confirms the hypothesis of a local production of SLPI in the mucous membranes.
authors.affiliation: Department of Otolaryngology and Head and Neck Surgery, Malmö General Hospital, University of Lund, Sweden.
authors.forename: U
authors.initials: U
authors.lastname: Westin
authors.forename: U
authors.initials: U
authors.lastname: Fryksmark
authors.forename: A
authors.initials: A
authors.lastname: Polling
authors.forename: K
authors.initials: K
authors.lastname: Ohlsson
mesh_terms: D056649:Cathepsin G; D002403:Cathepsins / Q000037:antagonists & inhibitors; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007962:Leukocytes / Q000166:cytology / Q000201:enzymology*; D009297:Nasal Mucosa / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors; D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis*; D053481:Secretory Leukocyte Peptidase Inhibitor; D012697:Serine Endopeptidases; D015842:Serine Proteinase Inhibitors / Q000032:analysis / Q000235:genetics*; D013194:Staining and Labeling
chemical_list: D053491:Proteinase Inhibitory Proteins, Secretory; D011506:Proteins; D015347:RNA Probes; D012333:RNA, Messenger; C506814:SLPI protein, human; D053481:Secretory Leukocyte Peptidase Inhibitor; D015842:Serine Proteinase Inhibitors; D002403:Cathepsins; D012697:Serine Endopeptidases; C534094:CTSG protein, human; D056649:Cathepsin G; D010196:Pancreatic Elastase