title: Expression of two types of nitric oxide synthase mRNA in human neuroblastoma cell lines.
abstract: Expression of nitric oxide synthase (NOS) was studied in nine human neuroblastoma and two human glioblastoma cell lines. Neuronal NOS (n-NOS) mRNA of approximately 10 kb was detected in four of the nine neuroblastoma cell lines by northern blot analysis using human n-NOS cDNA as a probe. Expression of the n-NOS mRNA was also detected in another neuroblastoma cell line in a subsequent reverse transcriptase polymerase chain reaction (RT-PCR) study, but no n-NOS mRNA expression was observed in the other four neuroblastoma cell lines or in the glioblastoma cell lines. The level of NOS activity correlated well with that of n-NOS mRNA expression in neuroblastoma cell lines expressing n-NOS mRNA. Western blot analysis showed that the n-NOS expressed in neuroblastoma cells was a 160-kDa protein reacted with anti-n-NOS antibody. By using the RT-PCR method, a short n-NOS (n-NOS-2) mRNA with a 315-bp inframe deletion from the entire n-NOS (n-NOS-1) mRNA was detected in the human neuroblastoma cells. The structural diversity of human n-NOS mRNA was demonstrated for the first time.
authors.affiliation: Biochemistry Division, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Fujisawa
authors.forename: T
authors.initials: T
authors.lastname: Ogura
authors.forename: Y
authors.initials: Y
authors.lastname: Kurashima
authors.forename: T
authors.initials: T
authors.lastname: Yokoyama
authors.forename: J
authors.initials: J
authors.lastname: Yamashita
authors.forename: H
authors.initials: H
authors.lastname: Esumi
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D005909:Glioblastoma / Q000737:chemistry / Q000201:enzymology / Q000473:pathology; D006801:Humans; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000737:chemistry* / Q000201:enzymology / Q000473:pathology*; D019001:Nitric Oxide Synthase; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases