Afferents to the nucleus reticularis parvicellularis of the cat medulla oblongata: a tract-tracing study with cholera toxin B subunit.
The aim of this study was to examine anatomical evidence in cats of whether the nucleus reticularis parvicellularis (Pc) is part of the circuit responsible for the inhibition of brainstem motoneurons during paradoxical sleep. For this purpose, we made iontophoretic injections of the retrograde and anterograde tracer cholera toxin B subunit (CTb) in the Pc. After CTb injections in the Pc, a large number of retrogradely labeled neurons were seen in the central nucleus of the amygdala, the lateral part of the bed nucleus of the stria terminalis, the posterior hypothalamic areas, the mesencephalic reticular formation, the nucleus locus subcoeruleus, the nucleus pontis caudalis, other portions of the Pc, the nucleus reticularis dorsalis, the trigeminal sensory complex, and the nucleus of the solitary tract. We further found that the Pc receives 1) serotoninergic afferents from the raphe dorsalis, magnus, and obscurus nuclei; 2) noradrenergic inputs from the dorsolateral pontine tegmentum; 3) cholinergic afferents from the lateral medullary reticular formation; 4) substance P-like afferents from the central nucleus of the amygdala, bed nucleus of the stria terminalis, periaqueductal gray, and nucleus of the solitary tract; and 5) methionine-enkephalin-like projections from the periaqueductal gray, the nucleus of the solitary tract, the lateral pontine and medullary reticular formation, and the spinal trigeminal nucleus. We further found that the Pc do not receive afferents from brainstem structures responsible for muscle atonia, such as the ventromedial medulla and the dorsomedial pontine tegmentum, and therefore may not be part of the circuit inhibiting the brainstem motoneurons during paradoxical sleep.
Fort
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Département de Médecine Expérimentale, U52 INSERM, URA 1195 CNRS, Université Claude Bernard, Lyon, France.
Luppi
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PH
Jouvet
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D000818:Animals; D002395:Catecholamines / Q000378:metabolism; D002415:Cats; D002772:Cholera Toxin / Q000276:immunology; D004744:Enkephalin, Methionine / Q000378:metabolism; D005260:Female; D007150:Immunohistochemistry; D007478:Iontophoresis; D008297:Male; D008526:Medulla Oblongata / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D009434:Neural Pathways / Q000166:cytology / Q000502:physiology; D009475:Neurons, Afferent / Q000378:metabolism / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000276:immunology / Q000378:metabolism; D010275:Parasympathetic Nervous System / Q000166:cytology / Q000502:physiology; D011149:Pons / Q000166:cytology / Q000502:physiology; D012154:Reticular Formation / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D012895:Sleep, REM / Q000502:physiology; D013373:Substance P / Q000378:metabolism
D002395:Catecholamines; D018377:Neurotransmitter Agents; D013373:Substance P; D004744:Enkephalin, Methionine; D002772:Cholera Toxin