Canine galanin: sequence, expression and pancreatic effects.
To determine if dog galanin is a potent inhibitor of dog insulin secretion we determined its primary structure from its cloned cDNA, evaluated its expression in celiac ganglia and determined its effect on islet hormone secretion. The predicted amino acid sequence differs from the other known species of galanin by three to six amino acids in the C-terminal half of the molecule. In situ hybridization revealed the presence of dog progalanin mRNA in every neuronal cell body in the dog celiac ganglion. The predicted dog galanin peptide was synthesized and infused i.v. at 0.25, 2.5, 25 or 250 pmol/kg/min. It potently inhibited insulin secretion, less potently inhibited pancreatic somatostatin release and stimulated glucagon secretion, similar to the effects of porcine galanin in the dog. In summary, dog galanin is expressed in the neuronal cell bodies that innervate the pancreas and the sequence of the dog galanin preserves the potent insulin inhibitory part of the galanin molecule. These data support the hypothesis that galanin is a sympathetic neurotransmitter in dog pancreas.
Boyle
M R
MR
Department of Medicine, University of Washington, Seattle 98108.
Verchere
C B
CB
McKnight
G
G
Mathews
S
S
Walker
K
K
Taborsky
G J
GJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001786:Blood Glucose / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry; D004285:Dogs; D019004:Galanin; D005728:Ganglia, Sympathetic / Q000378:metabolism*; D005934:Glucagon / Q000378:metabolism; D017403:In Situ Hybridization; D007328:Insulin / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology; D015345:Oligonucleotide Probes; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism; D010452:Peptide Biosynthesis; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D016133:Polymerase Chain Reaction; D013004:Somatostatin / Q000378:metabolism
D001786:Blood Glucose; D018076:DNA, Complementary; D007328:Insulin; D009479:Neuropeptides; D015345:Oligonucleotide Probes; D010455:Peptides; D013004:Somatostatin; D019004:Galanin; D005934:Glucagon