Expression of ICAM-1 on isolated human nasal, auricular and costal chondrocytes.
Expression of intercellular adhesion molecule-1 (ICAM-1) on targets has been reported to be a relevant factor for leukocyte migration, adhesion and function. Because stimulated chondrocytes have been shown to express molecules of immunological import (like HLA class II antigens) and because rejected or resorbed cartilage grafts used in the field of ENT are often characterized by adjacent infiltrating leukocytes, the presence of ICAM-1 on human nasal, auricular and costal cartilage was investigated. For this study, cartilage tissue sections and chondrocytes in suspension as well as cultured chondrocytes were prepared. Specific monoclonal antibodies (mAb) were used for immunocyto- and immunohistochemical Alkaline-Phosphatase-anti-Alkaline-Phosphatase staining (APAAP staining) as well as for flow cytometry analysis. ICAM-1 on healthy cartilage tissue sections was not found. On the other hand, both chondrocytes freed from matrix and cultured chondrocytes showed strongly positive staining patterns for ICAM-1. This result was obtained for chondrocytes from nasal, auricular as well as costal cartilage. This observed expression of ICAM-1 on chondrocytes with defective extracellular matrix demonstrates that cartilage cells are able to synthesize ICAM-1 without any paracrine stimulus from non-chondrocyte cells. It suggests that ICAM-1 plays a role in processes where tissue damage leads to the exposure of chondrocyte surfaces. Therefore, ICAM-1 expression on chondrocytes may also be a factor in destructive cartilage graft resorption.
Pitzke
P
P
Department of Otorhinolaryngology, Ludwig-Maximilians-University, Munich, Germany.
Bujía
J
J
Wilmes
E
E
Hammer
C
C
D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis* / Q000235:genetics; D000954:Antigens, Surface / Q000032:analysis; D002356:Cartilage / Q000737:chemistry* / Q000166:cytology; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000235:genetics; D002478:Cells, Cultured; D004425:Ear Cartilage / Q000737:chemistry* / Q000166:cytology; D004727:Endothelium / Q000737:chemistry / Q000166:cytology; D005347:Fibroblasts / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D009300:Nasal Septum / Q000737:chemistry* / Q000166:cytology; D012272:Ribs / Q000737:chemistry* / Q000166:cytology; D012867:Skin / Q000737:chemistry / Q000166:cytology; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1