Quantitative assay of trypsinogen by measurement of cleaved activation peptide after activation with enterokinase.
Measurement of trypsinogen by quantitative immunosorbent assay of the highly specific and inert trypsinogen activation peptide following complete activation of trypsinogen by enterokinase offers a simple and sensitive method that provides reliable results over a wide range of trypsinogen concentrations. This method offers a significant advantage in being applicable to complex biological tissues.
Mithöfer
K
K
Surgical Laboratories, Massachusetts General Hospital, Boston 02114-3139, USA.
Fernández-del Castillo
C
C
Mandavilli
U
U
Rattner
D W
DW
Warshaw
A L
AL
D000818:Animals; D002417:Cattle; D004765:Enteropeptidase / Q000494:pharmacology*; D010446:Peptide Fragments / Q000032:analysis*; D011817:Rabbits; D014362:Trypsinogen / Q000032:analysis*
D010446:Peptide Fragments; D014362:Trypsinogen; D004765:Enteropeptidase