VLA-2 mediates the interaction of collagen with endothelium during in vitro vascular tube formation.
A confluent endothelial monolayer can be induced to form vascular tubes in response to collagen. We investigated possible mechanisms of collagen-induced tube formation by using antibodies to the VLA-2 integrin receptor and protein kinase C inhibitors. Pre-incubation of cells with anti-VLA-2 (which recognises both the alpha 2 and beta 1 chains) and AK7 (which recognises only the alpha 2 chain) showed a dose-dependent inhibition of tube formation. At 50 micrograms/ml, anti-VLA-2 completely inhibited collagen-induced tube formation, whereas AK7 caused only partial inhibition. Both chlorpromazine and trifluoperazine, at concentrations of 10 microM, prevented tube formation (> 40% inhibition). In summary, the VLA-2 integrin receptor plays a role in the induction of tube formation by type I collagen. Protein kinase C may be activated during this process.
Jackson
C J
CJ
Department of Medicine, University of Sydney, NSW, Australia.
Knop
A
A
Giles
I
I
Jenkins
K
K
Schrieber
L
L
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000502:physiology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002746:Chlorpromazine / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000254:growth & development*; D006801:Humans; D019012:Integrin beta1; D016023:Integrins / Q000502:physiology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D038981:Receptors, Collagen; D012867:Skin / Q000166:cytology; D014268:Trifluoperazine / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D019012:Integrin beta1; D016023:Integrins; D009842:Oligopeptides; D038981:Receptors, Collagen; D014268:Trifluoperazine; C047981:arginyl-glycyl-aspartic acid; D003094:Collagen; D011493:Protein Kinase C; D002746:Chlorpromazine