Possible involvement of adenylylation in the modification of a 26 kDa protein in rat parotid acinar cells.
1. Adenylylation, a posttranslational modification of proteins, was investigated in saponin-permeabilized acinar cells of the rat parotid gland. 2. When cells were incubated with [2,8-3H]ATP, several proteins, including a 26 kDa protein in the particulate fraction, were labeled. 3. Upon incubation of cells with [alpha-32P]ATP in the presence of cAMP and 3-isobutyl-1-methylxanthine, 32P-labeling of the 26 kDa protein was observed. 4. After treatment with snake venom phosphodiesterase, [32P]AMP was released from the 26 kDa protein. Such release was not observed when cells were labeled with [gamma-32P]ATP. 5. The 32P-labeling pattern of proteins with [alpha-32P]ATP was clearly different from that with [adenylate-32P]NAD+. 6. The results suggest that the 26 kDa protein is one of the adenylylation substrates in rat parotid acinar cells.
Hara-Yokoyama
M
M
Department of Physiology, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Sugiya
H
H
Furuyama
S
S
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D002463:Cell Membrane Permeability; D000242:Cyclic AMP / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007553:Isotope Labeling; D008970:Molecular Weight; D010306:Parotid Gland / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology; D010761:Phosphorus Radioisotopes; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D012910:Snake Venoms / Q000201:enzymology; D013379:Substrate Specificity
D010761:Phosphorus Radioisotopes; D011506:Proteins; D012910:Snake Venoms; D000246:Adenosine Diphosphate Ribose; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D015056:1-Methyl-3-isobutylxanthine