Calcium mobilisation controls tyrosine protein phosphorylation independently of the activation of protein kinase C in human platelets.
We have investigated the regulation of tyrosine proteins phosphorylation by intracellular Ca2+ level ([Ca2+]i) and protein kinase C (PKC) during platelet stimulation. We found that chelation of extracellular calcium completely prevented phosphorylation of tyrosine proteins induced by thapsigargin and phorbol 12-myristate 13-acetate (PMA), whereas, when induced by thrombin, it prevented a subset of tyrosine proteins. The selective inhibition of PKC by GF 109203X did not abolish tyrosine protein phosphorylation when induced by thrombin and thapsigargin. The results suggest that in human platelets tyrosine protein phosphorylation is dependent on [Ca2+]i, although direct PKC activation can also induce phosphorylation of tyrosine proteins.
Falet
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H
INSERM CJF 91-01, Université René Descartes, Paris, France.
Rendu
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F
D001792:Blood Platelets / Q000502:physiology*; D002118:Calcium / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D004789:Enzyme Activation; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D008301:Maleimides / Q000494:pharmacology; D010766:Phosphorylation; D019000:Phosphotyrosine; D015539:Platelet Activation; D010974:Platelet Aggregation; D011493:Protein Kinase C / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D015398:Signal Transduction; D013729:Terpenes / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D019284:Thapsigargin; D013917:Thrombin / Q000494:pharmacology; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000096:biosynthesis
D007211:Indoles; D008301:Maleimides; D013729:Terpenes; D019000:Phosphotyrosine; D014443:Tyrosine; D019284:Thapsigargin; D004492:Edetic Acid; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D013917:Thrombin; C070515:bisindolylmaleimide I; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium