Detection of an "epimastigote-like" intracellular stage of Trypanosoma cruzi.
Monoclonal antibody (mAb) DION 5.1b, derived from mice immunized with Trypanosoma dionisii, recognizes a 72/76-kD surface glycoprotein specific to the epimastigote stage of T. dionisii and T. cruzi. None of the three other stages of the T. cruzi life cycle expresses any DION 5.1b-specific epitope. However, mAb DION 5.1b labels an intracellular form with "epimastigote-like" morphology that appears to be late and transient in the intracellular cycle. This result suggests that the morphological similarity between the observed "epimastigote-like" intracellular form in mammals and the epimastigote form in insects may extent to the antigenic pattern.
Faucher
J F
JF
INSERM U394, Bordeaux, France.
Baltz
T
T
Petry
K G
KG
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000913:Antibodies, Protozoan / Q000276:immunology*; D000947:Antigens, Helminth; D000953:Antigens, Protozoan / Q000032:analysis / Q000276:immunology*; D015153:Blotting, Western; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D008562:Membrane Glycoproteins / Q000276:immunology*; D014349:Trypanosoma cruzi / Q000254:growth & development* / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000947:Antigens, Helminth; D000953:Antigens, Protozoan; D000939:Epitopes; D008562:Membrane Glycoproteins