Estradiol-17 beta regulates the induction of VCAM-1 mRNA expression by interleukin-1 beta in human umbilical vein endothelial cells.
We examined the effect of estradiol-17 beta on the expression of vascular cell adhesion molecule-1 (VCAM-1), an adhesion molecule, in human umbilical vascular endothelial cells. After preincubation with estradiol-17 beta for 24 hours, cells were treated for 4 h with 0.5 micrograms/ml recombinant human interleukin-1 beta. The RNase protection assay was performed using an [alpha-32P]-labeled 121 base pair VCAM-1 cRNA probe. Preincubation with estradiol-17 beta (250 or 500 pg/ml) suppressed the induction of VCAM-1 mRNA expression by interleukin-1 beta. VCAM-1 staining with a monoclonal antibody decreased when cells were incubated with estradiol-17 beta at 250 and 500 pg/ml, while staining was detectable when cells were treated with interleukin-1 beta at 0.5 micrograms/ml. In conclusion, estradiol-17 beta regulates the induction of VCAM-1 gene expression by interleukin-1 beta in human umbilical vein endothelial cells.
Nakai
K
K
Second Department of Internal Medicine, Iwate Medical University, Morioka, Japan.
Itoh
C
C
Hotta
K
K
Itoh
T
T
Yoshizumi
M
M
Hiramori
K
K
D000911:Antibodies, Monoclonal; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000235:genetics*; D002478:Cells, Cultured; D004347:Drug Interactions; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D007700:Kinetics; D008969:Molecular Sequence Data; D010761:Phosphorus Radioisotopes; D012313:RNA / Q000032:analysis / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D014471:Umbilical Veins / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D019010:Vascular Cell Adhesion Molecule-1
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D007375:Interleukin-1; D010761:Phosphorus Radioisotopes; D015347:RNA Probes; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; D004958:Estradiol; D012313:RNA