Antigen-specific stimulation of histamine releasing factors in diisocyanate-induced occupational asthma.
Diisocyanate-induced asthma differs from occupational asthma (OA) caused by protein allergens in that specific IgE antibody responses are rarely identified. To investigate the immunopathogenesis of diisocyanate asthma, diisocyanate-exposed workers were evaluated for in vitro production of antigen-specific mononuclear cell-derived histamine releasing factor (HRF). The mean HRF response to diisocyanate-HSA antigens was significantly greater in patients with OA than in diisocyanate-exposed asymptomatic subjects (p < 0.05). No association was found between HRF and diisocyanate-specific antibodies. Analysis of HRF production by subpopulations of peripheral blood mononuclear cells (PBMC) showed that lymphocytes and adherent cells were major sources of both spontaneous and antigen-stimulated HRF. The results suggest that antigen-specific HRF produced by PBMCs are an important biomarker for diisocyanate-induced asthma. This is the first report of hapten-specific stimulation of PBMCs resulting in HRF production.
Herd
Z L
ZL
Department of Internal Medicine, University of Cincinnati College of Medicine, Ohio.
Bernstein
D I
DI
D000485:Allergens / Q000009:adverse effects*; D000918:Antibody Specificity / Q000187:drug effects / Q000276:immunology; D001249:Asthma / Q000139:chemically induced* / Q000453:epidemiology / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D014408:Biomarkers, Tumor; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes / Q000187:drug effects* / Q000276:immunology; D005260:Female; D006636:Histamine Release / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D017953:Isocyanates / Q000009:adverse effects*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008222:Lymphokines / Q000032:analysis / Q000187:drug effects* / Q000276:immunology; D008297:Male; D009784:Occupational Diseases / Q000139:chemically induced* / Q000453:epidemiology / Q000276:immunology; D018709:Statistics, Nonparametric; D014051:Toluene 2,4-Diisocyanate / Q000009:adverse effects*; D000090324:Tumor Protein, Translationally-Controlled 1; D001559:Benzhydryl Compounds
D000485:Allergens; D015415:Biomarkers; D014408:Biomarkers, Tumor; D000939:Epitopes; D017953:Isocyanates; D008222:Lymphokines; D014051:Toluene 2,4-Diisocyanate; D000090324:Tumor Protein, Translationally-Controlled 1; C005969:4,4'-diphenylmethane diisocyanate; D001559:Benzhydryl Compounds