title: A 48-kilodalton Mycoplasma fermentans membrane protein induces cytokine secretion by human monocytes.
abstract: Mycoplasma fermentans is one of several Mycoplasma species that have been reported to stimulate tumor necrosis factor (TNF) secretion from monocytes. This activity has been associated primarily with the mycoplasma membrane fraction. In this article, we have characterized a membrane protein that stimulates TNF and interleukin 1 beta secretion. The TNF-releasing activity partitioned into the Triton X-114 detergent phase, suggesting that the molecules is hydrophobic. The secretion of TNF is elevated in the presence of serum, which suggests that a serum component may play a role in the interaction between this mycoplasma protein and monocytes. Treatment of monocytes with monoclonal anti-CD14 antibody had no effect on the levels of TNF-releasing activity. By using the monocyte Western blot (immunoblot) technique, we have determined the molecular mass of the active molecule to be 48 kDa. This molecule appears to be distinct from the recently described family of variable lipoproteins of M. fermentans. Mycoplasma particulate material treated with proteinase K lost all inducing activity, whereas lipoprotein lipase-treated samples retained some level of activity.
authors.affiliation: Laboratory of Macrophage Biology, Guthrie Foundation for Medical Research, Sayre, Pennsylvania 18840.
authors.forename: D A
authors.initials: DA
authors.lastname: Kostyal
authors.forename: G H
authors.initials: GH
authors.lastname: Butler
authors.forename: D H
authors.initials: DH
authors.lastname: Beezhold
mesh_terms: D015703:Antigens, CD / Q000502:physiology; D015214:Antigens, Differentiation, Myelomonocytic / Q000502:physiology; D001426:Bacterial Proteins / Q000502:physiology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D018950:Lipopolysaccharide Receptors; D008565:Membrane Proteins / Q000502:physiology*; D008970:Molecular Weight; D009000:Monocytes / Q000378:metabolism*; D016858:Mycoplasma fermentans / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D001426:Bacterial Proteins; D016207:Cytokines; D018950:Lipopolysaccharide Receptors; D008565:Membrane Proteins; D014409:Tumor Necrosis Factor-alpha