Structure of the O-specific side chain of the lipopolysaccharide from Escherichia coli O126.
The polysaccharide isolated from Escherichia coli O126 lipopolysaccharide contains D-galactose, D-mannose, L-fucose, and 2-acetamido-2-deoxy-D-glucose in the molar ratios 1:1:1:1. The structure of the O-antigen of the lipopolysaccharide from E. coli O126 was established by compositional analysis, partial degradation, methylation analysis, and nuclear magnetic resonance spectroscopy. These studies revealed that the O-antigen is branched and built up of tetrasaccharide repeating units having the following structure: [formula: see text]
Bhattacharyya
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Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Calcutta.
Basu
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D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006868:Hydrolysis; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification
D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial