Anticardiolipin antibodies recognize beta 2-glycoprotein I structure altered by interacting with an oxygen modified solid phase surface.
Anticardiolipin antibodies (aCL) derived from the sera of individuals exhibiting the antiphospholipid syndrome (APS) directly bind to beta 2-glycoprotein I (beta 2-GPI), which is adsorbed to an oxidized polystyrene surface. Oxygen atoms were introduced on a polystyrene surface by irradiation with electron or gamma-ray radiation. X-ray photoelectron spectroscopy revealed the irradiated surfaces were oxidized to generate C-O and C = O moieties. aCL derived from either APS patients or (NZW x BXSB)F1 mice bound to beta 2-GPI coated on the irradiated plates, depending on the radiation dose. Antibody binding to beta 2-GPI on the irradiated plates was competitively inhibited by simultaneous addition of cardiolipin (CL)-coated latex beads mixed together with beta 2-GPI but were unaffected by addition of excess beta 2-GPI, CL micelles, or CL-coated latex beads alone. There was a high correlation between binding values of aCL in sera from 40 APS patients obtained by the anti-beta 2-GPI enzyme-linked immunosorbent assay (ELISA) using the irradiated plates and those by the beta 2-GPI-dependent aCL ELISA. Therefore, aCL have specificity for an epitope on beta 2-GPI. This epitope is expressed by a conformational change occurring when beta 2-GPI interacts with an oxygen-substituted solid phase surface.
Matsuura
E
E
Immunology Laboratory, Yamasa Corporation, Choshi, Japan.
Igarashi
Y
Y
Yasuda
T
T
Triplett
D A
DA
Koike
T
T
D000818:Animals; D000918:Antibody Specificity; D016736:Antiphospholipid Syndrome / Q000276:immunology; D002308:Cardiolipins / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology*; D006801:Humans; D051379:Mice; D010100:Oxygen / Q000737:chemistry*; D011137:Polystyrenes; D053482:beta 2-Glycoprotein I
D002308:Cardiolipins; D000939:Epitopes; D006023:Glycoproteins; D011137:Polystyrenes; D053482:beta 2-Glycoprotein I; D010100:Oxygen