Expression of CZ-1: a CD45RB epitope on progenitors of natural killer and other haematopoietic cells.
CZ-1 is a novel sialic acid-dependent epitope of the murine CD45RB molecule which is expressed on cells that proliferate when cultured in IL-2. Because IL-2 appears to be important in the differentiation of NK cells, the authors examined the expression of CZ-1 on immature NK-lineage cells within the bone marrow. All mature NK1.1+ cells as well as their NK1.1- IL-2 responsive precursors were CZ-1+. Furthermore, IL-2 unresponsive transplantable NK progenitor cells expressed CZ-1 also. To examine expression of CZ-1 on other immature lymphoid progenitor cells, CZ-1+ and CZ-1- marrow cells were transplanted into lightly irradiated scid mice. Transfer of CZ-1+ cells resulted in rapid and sustained generation of thymocytes and splenic B cells, whereas CZ-1- cells caused delayed repopulation. This suggested that the slowly repopulating pluripotent stem cells lacked CZ-1. Therefore, expression of CZ-1 on Ly6+ Lin- c-kit+ cells, highly enriched for pluripotent stem cells, was examined. This population appeared to be homogeneously CZ-1dull. Thus, it appears that expression of CZ-1 is developmentally regulated, with differentiation associated with increased expression. Since CZ-1 is expressed on a protein tyrosine phosphatase, it is likely that this molecule regulates differentiation of NK and other lymphoid cells.
Moore
T A
TA
Graduate Program in Immunology, University of Texas Southwestern Medical Center, Dallas 75235-9072.
Vargas-Cortes
M
M
Welsh
R M
RM
Bennett
M
M
Kumar
V
V
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D017690:Cell Transplantation / Q000502:physiology; D000939:Epitopes / Q000187:drug effects / Q000276:immunology*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D007376:Interleukin-2 / Q000502:physiology; D007694:Killer Cells, Natural / Q000166:cytology* / Q000637:transplantation; D017493:Leukocyte Common Antigens / Q000276:immunology; D008297:Male; D051379:Mice; D016513:Mice, SCID; D010641:Phenotype; D012794:Sialic Acids / Q000494:pharmacology; D013234:Stem Cells / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007376:Interleukin-2; D012794:Sialic Acids; D017493:Leukocyte Common Antigens