Inducible stable DNA replication of Escherichia coli tolerates unexcised pyrimidine dimers in an uvr-dependent manner.
Damage-inducible DNA replication (iSDR) was followed in UV-irradiated E. coli uvr+ and uvr B5 cells. Owing to the inhibition of dimer excision in the former (caused by the metabolic treatment), both contained similar amounts of unexcised dimers. Since the iSDR took place in uvr+ but not in uvr B5 cells, it is concluded that the uvr system can tolerate unexcised dimers through the recombinogenic iSDR.
Sedliaková
M
M
Department of Molecular Genetics, Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Slezáriková
V
V
Masek
F
F
Pirsel
M
M
D004249:DNA Damage; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics* / Q000528:radiation effects; D011740:Pyrimidine Dimers; D011836:Radiation Tolerance; D013941:Thymine / Q000378:metabolism; D013997:Time Factors; D014466:Ultraviolet Rays
D011740:Pyrimidine Dimers; D013941:Thymine