Neutrophils roll on E-selectin.
Using flow conditions that simulate those in post capillary venules, we have found that neutrophils attach and roll on a substrate bearing purified E-selectin. E-selectin resembles P-selectin (CD62) with regard to the dependence of attachment efficiency on wall shear stress and selectin density. In contrast, once attached, neutrophils form rolling adhesions on E-selectin that are much stronger than those on P-selectin. Rolling velocities on E-selectin are slower and have less variance than on P-selectin. With increasing shear stress, rolling velocities reach a plateau level that is dependent on E-selectin density, suggesting that the number of receptor-ligand bonds and the bond dissociation rate limit rolling velocity, and that the bonds are not broken by the applied force.
Lawrence
M B
MB
Center for Blood Research, Harvard Medical School, Boston, MA 02115.
Springer
T A
TA
D000255:Adenosine Triphosphate / Q000502:physiology; D000818:Animals; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000502:physiology*; D019040:E-Selectin; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1; D051379:Mice; D009504:Neutrophils / Q000502:physiology*; D013696:Temperature
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D018799:Intercellular Adhesion Molecule-1; D000255:Adenosine Triphosphate; D004492:Edetic Acid