title: Mast cell tryptase levels in normal canine tissues.
abstract: Levels of canine tryptase from various tissues were quantified using a competition enzyme-linked immunosorbent assay (ELISA). The assay utilises an affinity-purified rabbit anti-tryptase antibody in the solid phase and alkaline-phosphatase conjugated tryptase together with unlabelled tryptase in the fluid phase. The assay will rapidly quantify 40-5000 ng ml-1 of tryptase in tissue extracts. Tissues from the skin, gut, liver and lung were studied, of which canine gut appeared to contain the highest levels of tryptase per milligram wet weight, which may suggest an important role for this enzyme at this site. This assay may prove valuable in assessing the role of mast cells in various disease states in the dog.
authors.affiliation: Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, UK.
authors.forename: A D
authors.initials: AD
authors.lastname: Myles
authors.forename: R E
authors.initials: RE
authors.lastname: Halliwell
authors.forename: B
authors.initials: B
authors.lastname: Ballauf
authors.forename: H R
authors.initials: HR
authors.lastname: Miller
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002846:Chromatography, Affinity / Q000662:veterinary; D053818:Chymases; D004283:Dog Diseases / Q000201:enzymology; D004285:Dogs / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015151:Immunoblotting / Q000662:veterinary; D018836:Inflammation Mediators / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D007421:Intestine, Small / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008407:Mast Cells / Q000201:enzymology*; D012515:Mast-Cell Sarcoma / Q000201:enzymology / Q000662:veterinary; D012697:Serine Endopeptidases / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D012867:Skin / Q000201:enzymology; D053802:Tryptases
chemical_list: D018836:Inflammation Mediators; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases