Hemagglutination activity of Lactobacillus acidophilus group lactic acid bacteria.
The cells of 28 strains of the Lactobacillus acidophilus group were evaluated for hemagglutination (HA) activity. The activity was found in the surface layer (SL) protein fraction extracted by 2 M guanidine hydrochloride. The most SL proteins from the A group strains (L. acidophilus (A1), L. crispatus (A2), L. amylovorus (A3), and L. gallinarum (A4)) showed HA activity, but the proteins from the B group strains (L. gasseri (B1) and L. johnsonii (B2)) showed no activity. The SL proteins from the A group strains were composed in common of a main component having molecular mass of about 40-45 kDa on SDS-PAGE. The SL proteins from JCM 1034 strain that showed the highest HA activity was fractionated by CM-Toyopearl ion-exchange chromatography. The highest HA activity was detected in the major protein of 41 kDa. This protein was purified and shown to be composed of about 50% of hydrophobic amino acids. The HA activity of the protein (1034 lectin) was specifically inhibited by fetuin and bovine lactoferrin at the concentrations of 80 and 160 micrograms/ml, respectively. The removal of N-acetylneuraminic acid from fetuin significantly decreased the inhibitory activity.
Yamada
M
M
Laboratory of Animal Products Chemistry, College of Agriculture, Tohoku University, Sendai, Japan.
Saito
T
T
Toba
T
T
Kitazawa
H
H
Uemura
J
J
Itoh
T
T
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology; D001665:Binding Sites; D001709:Biotechnology; D002240:Carbohydrate Sequence; D002364:Caseins / Q000737:chemistry / Q000378:metabolism; D006020:Glycopeptides / Q000737:chemistry / Q000378:metabolism; D006384:Hemagglutination; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D066298:In Vitro Techniques; D007779:Lactobacillus acidophilus / Q000737:chemistry / Q000145:classification / Q000276:immunology*; D007781:Lactoferrin / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology; D013045:Species Specificity; D000509:alpha-Fetoproteins / Q000737:chemistry / Q000378:metabolism
D000596:Amino Acids; D001426:Bacterial Proteins; D002364:Caseins; D006020:Glycopeptides; D006388:Hemagglutinins; D009844:Oligosaccharides; D000509:alpha-Fetoproteins; D007781:Lactoferrin