Inhibitory effects of 2'-deoxy-L-thymidine 5'-triphosphate on human immunodeficiency virus reverse transcriptase and eukaryotic DNA polymerases.
Inhibitory effects of 2'-deoxy-L-thymidine 5'-triphosphate (L-dTTP) which is an enantiomer of the natural substrate (D-dTTP) on the activity of mammalian DNA polymerases, Escherichia coli DNA polymerase I and HIV-1 reverse transcriptase were examined. Interestingly, L-dTTP showed remarkable inhibitory effect on HIV-1 reverse transcriptase in competitive fashion with respect to the substrate dTTP. In contrast, eukaryotic cell nuclear DNA polymerases alpha and beta were not or slightly inhibited by L-dTTP.
Yamaguchi
T
T
Department of Biological Sciences, Nishi-Tokyo University, Yamanashi, Japan.
Saneyoshi
M
M
Shudo
K
K
D004256:DNA Polymerase I / Q000037:antagonists & inhibitors; D004926:Escherichia coli / Q000201:enzymology; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology*; D019384:Nucleic Acid Synthesis Inhibitors; D018894:Reverse Transcriptase Inhibitors; D013379:Substrate Specificity; D013942:Thymine Nucleotides / Q000378:metabolism / Q000494:pharmacology*
D019384:Nucleic Acid Synthesis Inhibitors; D018894:Reverse Transcriptase Inhibitors; D013942:Thymine Nucleotides; D054303:HIV Reverse Transcriptase; D004256:DNA Polymerase I; C024157:thymidine 5'-triphosphate