The detection of a breast cancer antigen on MCF-7 cells reactive with the TCR (alpha) of a specific killer T cell line.
We established several immortalized human T cell lines by cotransfecting primary lymphocytes derived from breast cancer patients with human c-myc and human c-Ha-ras oncogenes. A CD8 positive (CD8+) killer T cell line, FT-8, exhibited in vitro specific cytotoxicity to a human breast cancer cell line, MCF-7. The FT-8 cells suppressed the growth of MCF-7 cells transplanted to athymic mice. The cytotoxic reaction was caused via T cell antigen receptor (TCR) on MCF-7 cells, because monoclonal antibodies against the TCR inhibited the cytotoxicity of FT-8 cells. The TCR (alpha) cDNA of FT-8 was cloned by using a PCR amplification technique and expressed by a cell-free in vitro translation system. The TCR (alpha) protein recognized a target antigen of 32 KDa on MCF-7 cells.
Okano
F
F
Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.
Tachibana
H
H
Akiyama
K
K
Murakami
H
H
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology*; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000276:immunology* / Q000473:pathology / Q000628:therapy; D016827:CD8 Antigens / Q000276:immunology; D002460:Cell Line; D002461:Cell Line, Transformed; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D005434:Flow Cytometry; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009857:Oncogenes / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000032:analysis / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000951:Antigens, Neoplasm; D016827:CD8 Antigens; D000939:Epitopes; D011948:Receptors, Antigen, T-Cell