Germ-line and somatic mutations of the APC gene in patients with Turcot syndrome and analysis of APC mutations in brain tumors.
The Turcot syndrome (TS) is a rare, probably autosomal recessive, disorder characterized by development of primary neuroepithelial tumors of the central nervous system (CNS) and numerous adenomatous colorectal polyps. To examine the possible involvement of mutations of the APC gene, which is responsible for familial adenomatous polyposis (FAP), in Turcot syndrome, we examined DNAs from TS patients for alterations in this gene by means of ribonuclease protection analysis. Germ-line APC mutations were detected in each of three unrelated cases of TS, and additional (somatic) mutations were observed in colonic adenomas that had developed in one of these patients. However, no somatic mutations in APC were found among 91 neuroepithelial tumors (medulloblastoma, glioblastoma, astrocytoma, and oligodendroglioma), whether sporadic or associated with TS. These results suggest that the APC gene is associated with pathogenesis of one feature of TS, but that at least one other gene is responsible for the genesis of neuroepithelial tumors in the CNS.
Mori
T
T
Department of Biochemistry, Cancer Institute, Tokyo, Japan.
Nagase
H
H
Horii
A
A
Miyoshi
Y
Y
Shimano
T
T
Nakatsuru
S
S
Aoki
T
T
Arakawa
H
H
Yanagisawa
A
A
Ushio
Y
Y
D011125:Adenomatous Polyposis Coli / Q000235:genetics*; D000328:Adult; D001254:Astrocytoma / Q000235:genetics*; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics; D002675:Child, Preschool; D005260:Female; D017491:Genes, APC; D005909:Glioblastoma / Q000235:genetics; D006801:Humans; D008297:Male; D008527:Medulloblastoma / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016609:Neoplasms, Second Primary / Q000235:genetics*; D009386:Neoplastic Syndromes, Hereditary / Q000235:genetics*; D009837:Oligodendroglioma / Q000235:genetics; D010051:Ovarian Neoplasms / Q000235:genetics; D016133:Polymerase Chain Reaction; D017384:Sequence Deletion; D013577:Syndrome