title: Decreased expression of the alpha subunit of Ca2+/ calmodulin-dependent protein kinase type II mRNA in the adult rat CNS following recurrent limbic seizures.
abstract: Calcium/calmodulin-dependent protein kinase type II (CamKII) is a ubiquitous brain enzyme implicated in a wide variety of neuronal processes. Understanding CamKII has become increasingly complicated with the recent identification of multiple gene transcripts coding for separate subunits. Previous studies have shown that mRNA for the alpha subunit of CamKII can be increased by reduction of afferent input. In this study we have examined the regulation of alpha CamKII mRNA following increased activity due to seizures. Using in situ hybridization with a cRNA probe against the rat alpha CamKII sequence we found reduced levels of hybridization following limbic seizures induced by lesions of the hilus of the dentate gyrus. Hybridization was most dramatically reduced in the granule cells of the dentate gyrus and the pyramidal cells of hippocampal region CA1. There were also significant reductions in hybridization in the superficial layers of neocortex and piriform cortex. In each of these region hybridization was decreased in the molecular layers which is consistent with the reported dendritic localization of alpha CamKII mRNA. All changes in mRNA content were transient, with maximal reductions at 24 h following lesion placement and a return to control levels by 96 h. These findings demonstrate the negative regulation of alpha CamKII mRNA by seizure activity and raise the possibility that synthesis of this kinase may be regulated by normal physiological activity.
authors.affiliation: Department of Biochemistry and Molecular Biology, Mayo Clinic Jacksonville, FL, USA.
authors.forename: K D
authors.initials: KD
authors.lastname: Murray
authors.forename: C M
authors.initials: CM
authors.lastname: Gall
authors.forename: D L
authors.initials: DL
authors.lastname: Benson
authors.forename: E G
authors.initials: EG
authors.lastname: Jones
authors.forename: P J
authors.initials: PJ
authors.lastname: Isackson
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002147:Calmodulin / Q000235:genetics*; D002490:Central Nervous System / Q000378:metabolism*; D018891:Dentate Gyrus / Q000378:metabolism; D015870:Gene Expression; D017403:In Situ Hybridization; D008297:Male; D011494:Protein Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures
chemical_list: D002147:Calmodulin; D012333:RNA, Messenger; D011494:Protein Kinases; D002118:Calcium