The effect of a single intraperitoneal dose of hrIL-1 alpha on substance P-, neurokinin A-, calcitonin gene-related peptide- and neuropeptide Y-like immunoreactivity in cerebrospinal fluid, plasma and knee joint synovial fluid in the rat.
Substance P (SP)-, neurokinin A (NKA)-, calcitonin gene-related peptide (CGRP)- and neuropeptide Y (NPY)-like immunoreactivity (-LI) was studied in rats' cerebrospinal fluid (CSF), plasma and synovial fluid (SF) from both knee joints at 2 and 24 h following an intraperitoneal administration of 0.05 ml human recombinant interleukin-1 alpha (i.p. hrIL-1 alpha) or saline. Increased or decreased levels of SP-, NKA and CGRP-LI were detected in CSF and plasma, whereas NPY-LI was unaffected. In SF only CGRP-LI increased bilaterally. There was a correlation in CGRP-LI content between plasma and CSF following i.p. hrIL-1 alpha but not between plasma and SF or CSF and SF. It can be concluded that (1) i.p. hrIL-1 alpha activates somatosensory afferents thereby increasing SP- and CGRP-LI content in CSF and plasma and NKA-LI in CSF; 2) i.p. hrIL-1 alpha induces a bilateral increase of CGRP-LI in SF which is not mediated through systemic circulation and is possibly a part of the general host defensive reaction.
Bileviciute
I
I
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Lundeberg
T
T
Ekblom
A
A
Theodorsson
E
E
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000008:administration & dosage / Q000494:pharmacology*; D007719:Knee Joint; D008297:Male; D015288:Neurokinin A / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D009478:Neuropeptide Y / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D010529:Peritoneal Cavity; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D013373:Substance P / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*; D013582:Synovial Fluid / Q000378:metabolism*
D007375:Interleukin-1; D009478:Neuropeptide Y; D011994:Recombinant Proteins; D013373:Substance P; D015288:Neurokinin A; D015740:Calcitonin Gene-Related Peptide