Expression of inducible nitric oxide synthase in cytomegalovirus-infected glial cells of retinas from AIDS patients.
The inducible isoform of nitric oxide synthase has been detected in cytomegalovirus (CMV)-infected retinas from acquired immunodeficiency syndrome (AIDS) patients by immunohistochemistry and NADPH-diaphorase staining. Subsequent immunohistochemistry using antibodies against CMV antigens and glial fibrillary acidic protein (GFAP) demonstrated that inducible NOS was localized in CMV-infected glial cells, particularly Müller cells. These findings indicate that inducible NOS is expressed in vivo in the human retina as a result of viral infection, and suggest that high levels of NO production might be involved in CMV-induced retinitis.
Dighiero
P
P
Unité de Recherches Gérontologiques, INSERM U118, Association Claude Bernard, Paris, France.
Reux
I
I
Hauw
J J
JJ
Fillet
A M
AM
Courtois
Y
Y
Goureau
O
O
D000163:Acquired Immunodeficiency Syndrome / Q000201:enzymology*; D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis*; D017726:Cytomegalovirus Retinitis / Q000201:enzymology*; D004790:Enzyme Induction / Q000502:physiology; D005904:Glial Fibrillary Acidic Protein / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009252:NADPH Dehydrogenase / Q000276:immunology / Q000378:metabolism; D009457:Neuroglia / Q000201:enzymology*; D019001:Nitric Oxide Synthase; D012160:Retina / Q000166:cytology / Q000201:enzymology*
D005904:Glial Fibrillary Acidic Protein; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase