Immune response related to the molecular structure of a peptide from the cholera toxin B subunit.
Three forms of a peptide P50-75 from the cholera toxin B subunit in the absence of carrier or adjuvant were administered orally or intraperitoneally to C57Bl/6J mice. Mice were given P50-75 as the free monomer, as an octamer synthesized on the seven polylysine core proposed by Tam (S), or as an octamer synthesized on the epsilon-amino groups of a chain of eight Lys-Gly-Gly units (C). P50-75, presented to the immune system, as monomer or polymers, generated similar serum titres of anti-cholera toxin (CT) antibodies. However, mice immunized orally with the polymers S and C were better protected against the intestinal effects of the toxin than mice immunized with the free monomer P50-75. S and C are more effective than P50-75 or the B subunit in increasing the amounts of total IgA secreted into the intestine and, moreover, the anti-CT IgA neutralized toxin activity. The amounts of anti-CT subclasses (IgG1, IgG2a, IgG2b, and IgG3 plus IgM) produced by the antigens depended on how the peptide P50-75 was presented for priming to the mice boosted thereafter with the B subunit.
Halimi
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URA 163 CNRS, CHU Saint-Antoine, Paris, France.
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D000284:Administration, Oral; D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D000941:Antigens / Q000276:immunology / Q000494:pharmacology; D002772:Cholera Toxin / Q000138:chemical synthesis / Q000276:immunology / Q000494:pharmacology*; D000939:Epitopes / Q000276:immunology*; D007114:Immunization; D007070:Immunoglobulin A / Q000502:physiology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology / Q000378:metabolism; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology / Q000494:pharmacology*; D011487:Protein Conformation; D012995:Solubility
D000941:Antigens; D000939:Epitopes; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D046911:Macromolecular Substances; D010446:Peptide Fragments; C084765:cholera toxin B subunit (50-75); D002772:Cholera Toxin