title: Modified technique for efficient detection of microsporidia.
abstract: Changes in temperature (from room temperature to 50 degrees C) and staining time (from 90 to 10 min) were evaluated as a means of improving the detection of microsporidia from stool specimens. A blinded and independent comparison of 50 known positive matched-specimen pairs by three technologists resulted in consistently easier microscopic detection. The background is clearer, and spores stain more intensely. Staining time is reduced by 80 min.
authors.affiliation: McGill University Centre for Tropical Diseases, Montreal General Hospital, Canada.
authors.forename: E
authors.initials: E
authors.lastname: Kokoskin
authors.forename: T W
authors.initials: TW
authors.lastname: Gyorkos
authors.forename: A
authors.initials: A
authors.lastname: Camus
authors.forename: L
authors.initials: L
authors.lastname: Cedilotte
authors.forename: T
authors.initials: T
authors.lastname: Purtill
authors.forename: B
authors.initials: B
authors.lastname: Ward
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis*; D000818:Animals; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology; D006801:Humans; D016816:Microsporida / Q000302:isolation & purification*; D016881:Microsporidiosis / Q000175:diagnosis*; D010274:Parasitology / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors