title: Splenic lymphoma with villous lymphocytes: diagnosis by flow cytometry and immunocytochemistry.
abstract: Splenic lymphoma with villous lymphocytes (SLVL) is a recently described low-grade non-Hodgkin's lymphoma that may be confused with chronic lymphocytic leukemia and hairy cell leukemia. Herein we report a case of SLVL and show how clinical features, cellular morphology, and flow cytometric analysis are used to distinguish among these three disorders. A newly developed immunocytochemical technique that allows detection of tartrate-resistant acid phosphatase on formalin-fixed, paraffin-embedded specimens was useful in refining our diagnosis of SLVL. Establishing the precise diagnosis is important because treatment is different for each of the three chronic lymphoproliferative malignancies.
authors.affiliation: Department of Internal Medicine, University of Louisville, KY, USA.
authors.forename: M B
authors.initials: MB
authors.lastname: Brune
authors.forename: D L
authors.initials: DL
authors.lastname: Cain
authors.forename: E M
authors.initials: EM
authors.lastname: Cardwell
authors.forename: A J
authors.initials: AJ
authors.lastname: Janckila
mesh_terms: D000368:Aged; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000175:diagnosis* / Q000473:pathology; D008297:Male