Influence of the conformation of a macromolecule on the generation of T-cell proliferative response. A study with model polypeptides.
To study the influence of the conformation of polypeptidic macromolecules on the generation of T-cell epitopes, sequential polypeptides with an octamer repeat unit were designed and synthesized. They adopt mainly unordered and alpha-helical conformations. Among these polypeptides, those containing proline are fully or partly unordered, and are more effective at inducing T-cell proliferation than a proline-free very stable alpha-helical polypeptide. This extremely stable alpha-helical conformation, probably stabilized by aggregation, would enhance its stability against proteolytic processing.
Lacassie
E
E
Centre de Biophysique Moléculaire, CNRS, Orléans, France.
Delmas
A
A
Trudelle
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D000939:Epitopes / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000276:immunology*; D011392:Proline / Q000276:immunology; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D009842:Oligopeptides; D011392:Proline