Dioxin-receptor ligands in urban air and vehicle exhaust.
The ability of extracts of urban air and vehicle exhaust particulates to bind to the dioxin receptor has been determined. It was shown that such extracts do contain significant amounts of dioxin-receptor binding activity. The level of dioxin-receptor binding found in ambient air reflects its pollution level as determined by mutagenic activity. Furthermore, it was shown that the extracts of both urban air and vehicle exhaust particulates could provoke the induction of cytochrome P450IA1 in cultured rat hepatoma cells. Chemical fractionation of the extracts revealed that the majority of the dioxin-receptor binding activity from urban air and gasoline vehicle samples fractionated with the polycyclic aromatic compounds. However, unknown polycyclic aromatic compounds were responsible for the majority of the binding activity measured. In the case of diesel vehicle exhausts, the majority of the dioxin-receptor binding activity was found to be associated with nitro-polycyclic aromatic compounds. Studies with a variety of diesel fuels showed that the amount of dioxin-receptor ligands present in exhaust emissions are fuel-dependent and that substantial amounts of dioxin-receptor ligands are present in the semivolatile phase of exhaust emissions.
Mason
G G
GG
Department of Medical Nutrition, Karolinska Institute, Huddinge Hospital, Sweden.
D000393:Air Pollutants / Q000032:analysis*; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000096:biosynthesis; D005591:Chemical Fractionation; D008024:Ligands; D051381:Rats; D018336:Receptors, Aryl Hydrocarbon / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014504:Urban Health; D001335:Vehicle Emissions / Q000032:analysis*
D000393:Air Pollutants; D008024:Ligands; D018336:Receptors, Aryl Hydrocarbon; D001335:Vehicle Emissions; D001189:Aryl Hydrocarbon Hydroxylases