title: CD14 is expressed and functional in human B cells.
abstract: The human B cell line RPMI 8226 exhibits variable staining with the CD14 antibody My4. We have isolated three stable clones from this line with clones 1 and 2 being My4 positive and clone 3 My4 negative. Similar to previous results in monocytes, immunoprecipitation with the My4 antibody revealed a 54-kDa cell surface molecule, analysis of supernatants showed soluble CD14, and Northern blotting demonstrated a 1.4-kb transcript in clones 1 and 2, but not in clone 3, which suggests that the My4 antibody detects CD14 in clones 1 and 2. This CD14 molecule was functional in that lipopolysaccharide stimulation induced interleukin (IL)-6 and IL-10 in clones 1 and 2 but not in clone 3. Furthermore, the My4 antibody was capable of blocking these responses at the transcript and protein levels. Finally, peripheral blood B cells were highly purified by cell sorting (> 98% CD19 positive). These cells produced IL-6 in response to lipopolysaccharide, and this response was blocked by anti-CD14 antiserum. Thus, our findings demonstrated that human B cells can express functionally active CD14.
authors.affiliation: Institute for Immunology, University of Munich, Germany.
authors.forename: H W
authors.initials: HW
authors.lastname: Ziegler-Heitbrock
authors.forename: H
authors.initials: H
authors.lastname: Pechumer
authors.forename: I
authors.initials: I
authors.lastname: Petersmann
authors.forename: J J
authors.initials: JJ
authors.lastname: Durieux
authors.forename: N
authors.initials: N
authors.lastname: Vita
authors.forename: M O
authors.initials: MO
authors.lastname: Labeta
authors.forename: M
authors.initials: M
authors.lastname: Ströbel
mesh_terms: D015703:Antigens, CD / Q000096:biosynthesis* / Q000502:physiology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000096:biosynthesis* / Q000502:physiology*; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D006801:Humans; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides / Q000494:pharmacology*; D011233:Precipitin Tests; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D011971:Receptors, Immunologic