title: A conserved epitope on nuclear pore phosphoproteins reflects cell division status.
abstract: The nuclear pore complexes mediate the selective nuclear import of proteins in a signal- and energy-dependent process. We have earlier reported the characterization of a monoclonal antibody, Mab E2, that recognizes a novel class of nuclear pore phosphoproteins involved in signal-binding and protein transport. In the present study, we have analyzed the pattern of immunoreactivity of Mab E2 in cultured rat fibroblasts and have observed significant differences in the expression of epitopes in proliferating and quiescent cells. Furthermore, the common epitope recognized by Mab E2 is conserved across species, consistent with its essential role in nuclear protein import.
authors.affiliation: Centre for Cellular and Molecular Biology, Hyderabad.
authors.forename: K
authors.initials: K
authors.lastname: Mishra
authors.forename: K
authors.initials: K
authors.lastname: Divi
authors.forename: V K
authors.initials: VK
authors.lastname: Parnaik
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology*; D008969:Molecular Sequence Data; D009685:Nuclear Envelope / Q000737:chemistry; D010750:Phosphoproteins / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000939:Epitopes; D008565:Membrane Proteins; D010750:Phosphoproteins