High-performance liquid chromatographic determination of 3-hydroxy-3-methylglutaryl coenzyme A as diphenacyl ester of 3-hydroxy-3-methylglutarate.
A method for derivatization of (S)-3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) by phenacylation in tetrahexylammonium hydrogensulphate as phase-transfer reagent and high-performance liquid chromatography of the HMG-diphenacyl ester is described. To avoid interference with buffers and other ions used to dissolve biological material, the alkaline hydrolysate of CoA esters has to be neutralized with phosphoric rather than hydrochloric acid. The latter reacts with phenacyl bromide. The pH during phenacylation has to be maintained in the range 6-7 to provide sufficient dissociation of HMG.
Heintze
A
A
Botanisches Institut, Tierärztliche Hochschule, Hannover, Germany.
Schultz
G
G
D000214:Acyl Coenzyme A / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007202:Indicators and Reagents; D015093:Meglutol / Q000032:analysis*; D010944:Plants / Q000737:chemistry
D000214:Acyl Coenzyme A; D007202:Indicators and Reagents; C008047:3-hydroxy-3-methylglutaryl-coenzyme A; D015093:Meglutol