Distribution of integrin subunits in normal human kidney.
We evaluated on serial sections the distribution of a large number of integrin alpha and beta chains in normal adult human kidney: 1) the beta 1 chain and its corresponding alpha subunits (alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, alpha 6), 2) alpha v and beta 3 chains, 3) the beta 2 chain and its corresponding alpha chains (alpha X, alpha M, alpha L), and 4) the beta 4 chain. We also evaluated ICAM-1, VCAM and ELAM and the major extracellular matrix components (ECM). A three step immunoperoxidase technique was used on frozen sections. Each cell of the kidney shows a specific distribution of these molecules. The relation with ECM and some of their ligands was evaluated. This immunohistochemical study shows that there is no strict colocalisation of a given ECM component with its specific receptor.
Patey
N
N
Department of Nephrology, Hôpital Necker, Paris, France.
Halbwachs-Mecarelli
L
L
Droz
D
D
Lesavre
P
P
Noel
L H
LH
D000328:Adult; D000911:Antibodies, Monoclonal; D001485:Basement Membrane / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D019040:E-Selectin; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D007124:Immunoenzyme Techniques; D016023:Integrins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1; D007668:Kidney / Q000378:metabolism*; D007678:Kidney Glomerulus / Q000378:metabolism; D007684:Kidney Tubules / Q000378:metabolism; D008727:Methotrexate / Q000378:metabolism; D011487:Protein Conformation; D014018:Tissue Distribution; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D016023:Integrins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D008727:Methotrexate