Certain anti-CD34 monoclonal antibodies induce homotypic adhesion of leukemic cell lines in a CD18-dependent and a CD18-independent way.
CD34 is a highly glycosylated type I membrane protein expressed by early hematopoietic progenitor cells as well as by endothelial cells and a subset of bone marrow stromal cells. CD34 is thought to play an important role during early hematopoiesis, although its function is unknown. We demonstrate that triggering of CD34 results in a rapid and vigorous homotypic adhesion in CD34+ cell lines, thereby providing evidence for a cell-cell adhesion function of CD34. The cellular adhesion event, induced by only two anti-CD34 mAb, (Immu-133 and QBend-10) was dependent on metabolic energy, an intact cytoskeleton and the presence of divalent cations. Analysis of antibody inhibition experiments indicated that the aggregation process partially involved the CD18 molecule.
Traoré
Y
Y
Department of Monoclonal Antibodies, Marseille, France.
Hirn
J
J
D000911:Antibodies, Monoclonal / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D018952:Antigens, CD34; D018821:CD18 Antigens / Q000502:physiology*; D002448:Cell Adhesion; D002449:Cell Aggregation; D003599:Cytoskeleton / Q000502:physiology; D004734:Energy Metabolism; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D066298:In Vitro Techniques; D007938:Leukemia / Q000276:immunology* / Q000473:pathology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018952:Antigens, CD34; D018821:CD18 Antigens; D007140:Immunoglobulin Fab Fragments; D016169:Lymphocyte Function-Associated Antigen-1