title: Molecular and biological characterization of a non-glycosylated isolate of St Louis encephalitis virus.
abstract: The glycosylation patterns of the envelope (E) glycoprotein of several naturally occurring strains of St Louis encephalitis (SLE) virus were investigated. SLE viruses were found that contained both glycosylated and non-glycosylated E proteins, and one isolate (Tr 9464) that lacks N-linked glycosylation sites on its E protein was identified. SLE virus monoclonal antibodies that define E protein B cell epitopes and demonstrate biological activities reacted essentially to the same extent with glycosylated and non-glycosylated virions. These results indicate that glycosylation is not essential for epitope conformation or recognition. However, failure to glycosylate the E protein was associated with possible morphogenetic differences as manifested by reduced virus yields and differences in specific infectivity.
authors.affiliation: Center for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, San Juan, Puerto Rico 00921-3200.
authors.forename: V
authors.initials: V
authors.lastname: Vorndam
authors.forename: J H
authors.initials: JH
authors.lastname: Mathews
authors.forename: A D
authors.initials: AD
authors.lastname: Barrett
authors.forename: J T
authors.initials: JT
authors.lastname: Roehrig
authors.forename: D W
authors.initials: DW
authors.lastname: Trent
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D004665:Encephalitis Virus, St. Louis / Q000737:chemistry / Q000276:immunology* / Q000472:pathogenicity; D000939:Epitopes; D014644:Genetic Variation; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology*; D006031:Glycosylation; D006385:Hemagglutination Inhibition Tests; D051379:Mice; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D011487:Protein Conformation; D011499:Protein Processing, Post-Translational; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D013045:Species Specificity; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology*; D014774:Virulence
chemical_list: D000939:Epitopes; D006023:Glycoproteins; D014759:Viral Envelope Proteins