Immunohistochemical detection of substance P and vasoactive intestinal peptide fibres in the auricular lymph nodes of sensitized guinea pigs and mice.
The presence of substance P (SP) and vasoactive intestinal peptide (VIP) in auricular lymph nodes of normal and sensitized guinea pigs and mice has been investigated using polyclonal antibodies directed against these neurotransmitters and the unlabelled peroxidase-antiperoxidase procedure. Positive immunoreactivity could be observed only in draining lymph nodes of sensitized animals. SP-immunoreactivity was found in mice and guinea pigs but a VIP-positive reaction only in mice. The immunopositive sites were always associated with blood vessels. The present study suggests that the neurotransmitters SP and VIP are involved in mechanisms of sensitization taking place in secondary lymphoid organs.
Enzmann
V
V
Department of Biosciences, University of Leipzig, Germany.
Drössler
K
K
D000818:Animals; D004139:Dinitrofluorobenzene / Q000494:pharmacology; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000737:chemistry / Q000187:drug effects / Q000294:innervation*; D051379:Mice; D009412:Nerve Fibers / Q000737:chemistry* / Q000187:drug effects; D013373:Substance P / Q000032:analysis*; D014660:Vasoactive Intestinal Peptide / Q000032:analysis*
D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D004139:Dinitrofluorobenzene