Monoclonal antibody 1D8 detects an activation-related molecule on human B lymphocytes and on a minor T-lymphocyte subpopulation.
A novel murine monoclonal antibody (MAb) 1D8 was produced after immunization with the nylon wool-adherent fraction of human peripheral blood mononuclear cells (PBMNC). Its reactivity pattern was studied on a panel of hemopoetic normal cells, cell lines and malignancies. Mab 1D8 detects a lymphocyte-specific surface antigen expressed on a major subpopulation of mature B lymphocytes as well as on a minor T-cell subset. Activation-related changes in the expression of 1D8 molecule were observed on both B and T lymphocytes. As compared with the pattern of known activation-associated antigens, 1D8 seems to play a role in the early stages of lymphocyte activation. The antigen could not be immunoprecipitated by the conventional methods for glycoproteins.
Nikolova
M
M
National Center of Infectious and Parasitic Disease, Sofia, Bulgaria.
Taskov
H
H
D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D011233:Precipitin Tests; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000939:Epitopes