Function of the peripheral serotoninergic pathways in migraine: a proposal for an experimental model.
We propose a model for assessing the function of 5HT receptors in migraine by evaluating their expression on monocytes by means of double-labeling fluorocytometry (CD14-positive cells FITC-labeled). This model demonstrates that during headache induced in migraine without aura sufferers given isosorbide dinitrate followed by sumatriptan treatment 5HT expression on monocytes progressively increases. This increase may be due to the activation of 5HT turnover and to the increased availability of 5HT displaced by sumatriptan from cerebrovascular receptors during head pain.
Martelletti
P
P
Headache Center, University La Sapienza, Rome, Italy.
Stirparo
G
G
Rinaldi
C
C
Fusco
B M
BM
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D001667:Binding, Competitive; D005260:Female; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D007548:Isosorbide Dinitrate / Q000009:adverse effects; D018950:Lipopolysaccharide Receptors; D008297:Male; D008881:Migraine Disorders / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy / Q000503:physiopathology*; D009000:Monocytes / Q000737:chemistry* / Q000187:drug effects; D011985:Receptors, Serotonin / Q000187:drug effects / Q000502:physiology*; D012701:Serotonin / Q000378:metabolism / Q000502:physiology*; D018170:Sumatriptan / Q000378:metabolism / Q000627:therapeutic use*; D015854:Up-Regulation / Q000187:drug effects
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D018950:Lipopolysaccharide Receptors; D011985:Receptors, Serotonin; D012701:Serotonin; D018170:Sumatriptan; D016650:Fluorescein-5-isothiocyanate; D007548:Isosorbide Dinitrate