Breakpoints of Burkitt's lymphoma t(8;22) translocations map within a distance of 300 kb downstream of MYC.
The variant translocation t(8;22) in Burkitt's lymphoma (BL) cells joins band q24 of chromosome 8 distal to the proto-oncogene MYC to the immunoglobulin lambda locus. The distribution of breakpoints on chromosome 8 of 11 cell lines with t(8;22) has been investigated by in situ fluorescence hybridization and pulsed-field gel electrophoresis. We show that these chromosomal breakpoints generally fall within a region of about 300 kb 3' of MYC and that at least 8 out of 11 affect the previously characterized transcriptional unit PVT1. Comparable results were obtained in earlier experiments analyzing the variant t(2;8). Recently, in a series of BL cells carrying t(8;14), breakpoints upstream of MYC have been described at a similar distance. Therefore, our results suggest that deregulation of MYC by the immunoglobulin loci can occur at a distance of up to about 350 kbp of MYC.
Zeidler
R
R
Institut für Biochemie, Ludwig-Maximilians-Universität, München, Germany.
Joos
S
S
Delecluse
H J
HJ
Klobeck
G
G
Vuillaume
M
M
Lenoir
G M
GM
Bornkamm
G W
GW
Lipp
M
M
D002051:Burkitt Lymphoma / Q000235:genetics* / Q000473:pathology; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22 / Q000648:ultrastructure*; D002898:Chromosomes, Human, Pair 8 / Q000648:ultrastructure*; D004742:Enhancer Elements, Genetic; D015972:Gene Expression Regulation, Neoplastic; D005803:Genes, Immunoglobulin; D016259:Genes, myc; D006801:Humans; D007146:Immunoglobulin lambda-Chains / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D011401:Promoter Regions, Genetic; D000090063:Proto-Oncogene Mas; D014158:Transcription, Genetic; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D007146:Immunoglobulin lambda-Chains; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas