title: Histochemical and ultrastructural studies of mast cells in the intestinal mucosa and skin of the opossum Didelphis albiventris.
abstract: The mast cells located in the intestinal mucosa of a non-eutherian mammal were studied in comparison with those in the skin of the ear. In the intestinal mucosa, the mast cells exhibited a more variable shape, larger cytoplasmic granules and greater sensitivity to fixatives regarding their staining towards Toluidine Blue. Ultrastructurally, these granules were more heterogeneous but lacked the crystalline content found in granules of skin mast cells; lipid body-like organelles were found only in the mucosa-located mast cells. Histochemically, they differed from skin mast cells by the absence of periodic acid-Schiff (PAS)-positive granules. Unlike the mucosal mast cells of the rat, they fluoresced brilliant yellow after berberine treatment, which is evidence of the presence of heparin.
authors.affiliation: Departamento de Morfologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: A A
authors.initials: AA
authors.lastname: Santos
authors.forename: C R
authors.initials: CR
authors.lastname: Machado
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D048429:Cell Size; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004423:Ear; D006651:Histocytochemistry; D007413:Intestinal Mucosa / Q000166:cytology*; D008297:Male; D008407:Mast Cells / Q000737:chemistry / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009893:Opossums; D015388:Organelles / Q000648:ultrastructure; D012545:Schiff Bases; D012867:Skin / Q000166:cytology*; D013194:Staining and Labeling; D014048:Tolonium Chloride
chemical_list: D012545:Schiff Bases; D014048:Tolonium Chloride