A Drosophila homolog of the yeast origin recognition complex.
Genes from Drosophila melanogaster have been identified that encode proteins homologous to Orc2p and Orc5p of the Saccharomyces cerevisiae origin recognition complex (ORC). The abundance of the Drosophila Orc2p homolog DmORC2 is developmentally regulated and is greatest during the earliest stages of embryogenesis, concomitant with the highest rate of DNA replication. Fractionation of embryo nuclear extracts revealed that DmORC2 is found in a tightly associated complex with five additional polypeptides, much like the yeast ORC. These studies will enable direct testing of the initiator-based model of replication in a metazoan.
Gossen
M
M
Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
Pak
D T
DT
Hansen
S K
SK
Acharya
J K
JK
Botchan
M R
MR
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004261:DNA Replication; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D004331:Drosophila melanogaster / Q000737:chemistry / Q000196:embryology / Q000235:genetics*; D004625:Embryo, Nonmammalian / Q000737:chemistry; D017344:Genes, Insect; D008969:Molecular Sequence Data; D008970:Molecular Weight; D051738:Origin Recognition Complex; D018741:Replication Origin; D012097:Repressor Proteins / Q000032:analysis / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017385:Sequence Homology
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D051738:Origin Recognition Complex; D012097:Repressor Proteins