title: Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity.
abstract: A method is described for detecting yeast cells with plasma membrane damage, based on cell staining with bromocresol purple (BCP) which has a convenient fluorescence after entering the cells at pH below 5.2. The method was used to determine the activity of Saccharomyces cerevisiae pore-forming killer toxin K1 in commonly used lethal units. The BCP test is rapid and as precise as the plating test.
authors.affiliation: Institute of Microbiology, Czech Academy of Sciences, Prague.
authors.forename: H
authors.initials: H
authors.lastname: Kurzweilová
authors.forename: K
authors.initials: K
authors.lastname: Sigler
mesh_terms: D001962:Bromcresol Purple; D002462:Cell Membrane / Q000187:drug effects; D015169:Colony Count, Microbial / Q000379:methods*; D005456:Fluorescent Dyes; D055646:Killer Factors, Yeast; D009183:Mycotoxins / Q000032:analysis* / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000187:drug effects; D029701:Saccharomyces cerevisiae Proteins; D013194:Staining and Labeling / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D029701:Saccharomyces cerevisiae Proteins; D001962:Bromcresol Purple