title: Lysine residues in bee venom phospholipase A2 are important for binding to human monoclonal or polyclonal antibodies of the IgG4 isotope.
abstract: Discontinuous antigenic sites on bee venom phospholipase A2 (PLA) have been mapped using human monoclonal antibodies or human polyclonal serum antibodies (hpAbs) of the IgG4 isotype from beekeepers or of the IgE isotype from individuals allergic to PLA. Lysine residues of PLA have been specifically modified by acetylation or acylation by treatment with citraconic anhydride of their epsilon-amino groups to analyze their role in antigen-antibody binding. After the modifications, the binding of PLA to the human monoclonal antibodies is lost, whereas the binding to IgG4 hpAbs is significantly decreased. In contrast, the effect on the binding of PLA to IgE hpAbs appears to be more heterogeneous. The data indicate the importance of lysine residues as being part of B cell epitopes in PLA-specific antibodies of the IgG4 isotype, but less so for those of the IgE isotype.
authors.affiliation: Swiss Institute of Allergy and Asthma Research, Davos.
authors.forename: T
authors.initials: T
authors.lastname: Schneider
authors.forename: T
authors.initials: T
authors.lastname: Dudler
authors.forename: M H
authors.initials: MH
authors.lastname: Gelb
authors.forename: M
authors.initials: M
authors.lastname: Suter
mesh_terms: D000107:Acetylation; D000215:Acylation; D000818:Animals; D000906:Antibodies / Q000378:metabolism; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001402:B-Lymphocytes; D001514:Bee Venoms / Q000201:enzymology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000378:metabolism*; D007132:Immunoglobulin Isotypes / Q000378:metabolism*; D008239:Lysine / Q000737:chemistry / Q000502:physiology*; D051379:Mice; D010741:Phospholipases A / Q000737:chemistry / Q000378:metabolism*; D054467:Phospholipases A2; D013329:Structure-Activity Relationship
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D001514:Bee Venoms; D000939:Epitopes; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D010741:Phospholipases A; D054467:Phospholipases A2; D008239:Lysine