Heterogeneity of human anti-Golgi auto-antibodies: reactivity with components from 35 to 260 kDa.
The Golgi auto-antigens recognized by five human autoimmune sera were characterized with anti-Golgi auto-antibodies. The five sera showed strong anti-Golgi reactivity, together with weak anti-nuclear reactivity, as assessed by indirect immunofluorescence using the human HEp2 epithelial cell line. The Golgi auto-antigens recognized by the autoimmune sera were identified by immunoprecipitation and immunoblotting of post-nuclear supernatants. These sera reacted with at least 14 components of molecular mass (M(r)) 35-260 kDa; three components were detected only by immunoprecipitation, six components detected only by immunoblotting and five components appear to be detected by both techniques. We have previously shown that a patient with Sjögren's syndrome has auto-antibodies specific for a 230 kDa Golgi auto-antigens; a bacterial fusion protein containing auto-epitope(s) of this Golgi auto-antigen is not recognized by the other four autoimmune sera. Taken together, this study shows that the five anti-Golgi autoimmune sera recognize distinct sets of auto-antigens which include both conformational and/or sequential epitopes.
Kooy
J
J
Department of Pathology and Immunology, Monash University Medical School, Alfred Hospital, Melbourne, Victoria, Australia.
Toh
B H
BH
Gleeson
P A
PA
D001323:Autoantibodies / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium; D000939:Epitopes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006056:Golgi Apparatus / Q000276:immunology*; D006367:HeLa Cells; D006801:Humans; D015151:Immunoblotting; D008970:Molecular Weight; D010391:Pemphigoid, Bullous / Q000276:immunology; D012859:Sjogren's Syndrome / Q000276:immunology
D001323:Autoantibodies; D001324:Autoantigens; D000939:Epitopes