title: Identification of adenylyl cyclases by amplification using degenerate primers.
authors.affiliation: Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: R T
authors.initials: RT
authors.lastname: Premont
mesh_terms: D000262:Adenylyl Cyclases / Q000032:analysis* / Q000096:biosynthesis* / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D018076:DNA, Complementary / Q000096:biosynthesis; D004023:Dictyostelium / Q000201:enzymology; D004330:Drosophila / Q000201:enzymology; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D008322:Mammals; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D016133:Polymerase Chain Reaction / Q000379:methods*; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012194:RNA-Directed DNA Polymerase; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases