Modulation by fatty acids of Ca2+ fluxes in sarcoplasmic-reticulum vesicles.
The fatty acids palmitic (C16:0), stearic (C18:0), arachidic (C20:0) and arachidonic (C20:4) acids inhibit Ca2+ uptake and enhance Ca2+ efflux measured in vesicles derived from the sarcoplasmic reticulum of skeletal muscle. These effects of the fatty acids are impaired by the Ca(2+)-ATPase ligands Mg2+, Ca2+ and K+, and by drugs that block the leakage of Ca2+ through the Ca(2+)-ATPase such as Ruthenium Red, spermine [de Meis (1991) J. Biol. Chem. 266, 5736-5742] and thapsigargin [de Meis and Inesi (1992) FEBS Lett. 299, 33-35].
Cardoso
C M
CM
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brasil.
De Meis
L
L
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004537:Eicosanoic Acids / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D007700:Kinetics; D008094:Lithium / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D009132:Muscles / Q000378:metabolism*; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011817:Rabbits; D012430:Ruthenium Red / Q000494:pharmacology; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism*; D013096:Spermine / Q000494:pharmacology; D013229:Stearic Acids / Q000494:pharmacology; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin
D004537:Eicosanoic Acids; D005230:Fatty Acids, Nonesterified; D010169:Palmitic Acids; D013229:Stearic Acids; D013729:Terpenes; D012430:Ruthenium Red; D016718:Arachidonic Acid; D013096:Spermine; D019308:Palmitic Acid; C031183:stearic acid; D019284:Thapsigargin; D000255:Adenosine Triphosphate; D008094:Lithium; D000252:Calcium-Transporting ATPases; D008274:Magnesium; D011188:Potassium; D002118:Calcium