title: 32P-postlabelling analysis of DNA adducts in humans: adduct distribution and method improvement.
abstract: 32P-Postlabelling was applied to study the distribution of adducts in white blood cells of foundry workers exposed to polycylic aromatic hydrocarbons. The distribution of the adducts among 63 workers followed an apparently trimodal pattern, which could relate to polymorphism in PAH metabolism. A modified postlabelling method is described and some parameters were tested for optimal labelling. The total volume of the polynucleotide kinase reaction is 2 microliters, which decreases exposure to radioactivity and costs of isotopes.
authors.affiliation: Center for Nutrition and Toxicology, Karolinska Institute, Novum, Huddinge, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Szyfter
authors.forename: J
authors.initials: J
authors.lastname: Krüger
authors.forename: P
authors.initials: P
authors.lastname: Ericson
authors.forename: C
authors.initials: C
authors.lastname: Vaca
authors.forename: A
authors.initials: A
authors.lastname: Försti
authors.forename: K
authors.initials: K
authors.lastname: Hemminki
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D018736:DNA Adducts / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D016273:Occupational Exposure; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism; D011865:Radioisotope Dilution Technique
chemical_list: D018736:DNA Adducts; D010761:Phosphorus Radioisotopes; D001564:Benzo(a)pyrene; D011116:Polynucleotide 5'-Hydroxyl-Kinase