A comparison of human and murine monoclonal IgGs specific for the P1.7 PorA protein of Neisseria meningitidis.
Monoclonal human IgG SS269 reacts with Neisseria meningitidis expressing the P1.7 PorA protein and with linear peptides containing NGGAS, which accounts for the P1.7 specificity. Murine monoclonal antibody to P1.7 reacts with peptides containing the overlapping epitope, ASGQ. The human and murine antibodies have similar affinities. The low avidity human antibody is very inefficient at stimulating complement-mediated bactericidal killing while the high avidity murine antibody efficiently kills bacteria. However, efficient opsonophagocytosis was mediated even at low concentrations of the human antibody and in the absence of complement, suggesting that low avidity antibodies might be protective against disease.
Delvig
A
A
Max Planck Institut für molekulare Genetik, Berlin, Germany.
Jahn
S
S
Kusecek
B
B
Heckels
J E
JE
Rosenqvist
E
E
Høiby
E A
EA
Michaelsen
T E
TE
Achtman
M
M
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006801:Humans; D006825:Hybridomas; D007074:Immunoglobulin G / Q000276:immunology*; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000276:immunology*; D009895:Opsonin Proteins / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D010587:Phagocytosis; D018272:Porins / Q000276:immunology*; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007074:Immunoglobulin G; D009895:Opsonin Proteins; D010446:Peptide Fragments; D018272:Porins; C057699:porin protein, Neisseria