Modified technique for efficient detection of microsporidia.
Changes in temperature (from room temperature to 50 degrees C) and staining time (from 90 to 10 min) were evaluated as a means of improving the detection of microsporidia from stool specimens. A blinded and independent comparison of 50 known positive matched-specimen pairs by three technologists resulted in consistently easier microscopic detection. The background is clearer, and spores stain more intensely. Staining time is reduced by 80 min.
Kokoskin
E
E
McGill University Centre for Tropical Diseases, Montreal General Hospital, Canada.
Gyorkos
T W
TW
Camus
A
A
Cedilotte
L
L
Purtill
T
T
Ward
B
B
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis*; D000818:Animals; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology; D006801:Humans; D016816:Microsporida / Q000302:isolation & purification*; D016881:Microsporidiosis / Q000175:diagnosis*; D010274:Parasitology / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors