The effects of intraoperative vasodilators and angiographic contrast medium on the endothelium and smooth muscle cells of vein grafts.
Cellular injury is a major cause of intimal hyperplasia in vein grafts. The effects of exposure of vein samples to iopamidol, papaverine and iloprost were studied in vitro in an organ chamber to determine whether these agents cause endothelial and/or smooth muscle cell injury. Smooth muscle cell function was assessed by eliciting a dose response curve to noradrenaline. Endothelial cell function was assessed by measuring the degree of endothelial-dependent relaxation of sub-maximally contracted vein samples. Iopamidol and iloprost did not have any deleterious effect on endothelial or smooth muscle cell function. Papaverine did not affect endothelial-dependent relaxation but did produce a significant decrease in smooth muscle contraction. The use of these intraoperative agents during femorodistal bypass does not appear to cause functional injury to the endothelial or smooth muscle cells.
Sayers
R D
RD
Department of Surgery, Leicester Royal Infirmary, U.K.
Watt
P A
PA
Bell
P R
PR
Thurston
H
H
D000368:Aged; D001807:Blood Vessel Prosthesis; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects*; D006083:Graft Occlusion, Vascular / Q000517:prevention & control; D006801:Humans; D016285:Iloprost / Q000494:pharmacology*; D066298:In Vitro Techniques; D007479:Iopamidol / Q000494:pharmacology*; D008875:Middle Aged; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D009638:Norepinephrine / Q000494:pharmacology; D010208:Papaverine / Q000494:pharmacology*; D012501:Saphenous Vein / Q000187:drug effects / Q000637:transplantation
D010208:Papaverine; D016285:Iloprost; D007479:Iopamidol; D009638:Norepinephrine