Bell palsy and herpes simplex virus: identification of viral DNA in endoneurial fluid and muscle.
OBJECTIVE
To determine whether herpes simplex virus type 1 (HSV-1) causes Bell palsy.


METHODS
Prospective study.


METHODS
University inpatient service.


METHODS
14 patients with Bell palsy, 9 patients with the Ramsay-Hunt syndrome, and 12 other controls.


METHODS
Viral genomes of HSV-1, varicella-zoster virus, and Epstein-Barr virus were analyzed in clinical samples of facial nerve endoneurial fluid and posterior auricular muscle using polymerase chain reaction (PCR) followed by hybridization with Southern blot analysis.


RESULTS
Herpes simplex virus type 1 genomes were detected in 11 of 14 patients (79%) with Bell palsy but not in patients with the Ramsay-Hunt syndrome or in other controls. The nucleotide sequences of the PCR fragments were identical to those of the HSV-1 genome.


CONCLUSIONS
Herpes simplex virus type 1 is the major etiologic agent in Bell palsy.
Murakami
S
S
Ehime University School of Medicine, Japan.
Mizobuchi
M
M
Nakashiro
Y
Y
Doi
T
T
Hato
N
N
Yanagihara
N
N
D000328:Adult; D000368:Aged; D001483:Base Sequence; D015139:Blotting, Southern; D001826:Body Fluids / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000302:isolation & purification*; D005158:Facial Paralysis / Q000821:virology*; D005260:Female; D016679:Genome, Viral; D016697:Herpes Zoster Oticus / Q000503:physiopathology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000821:virology*; D016133:Polymerase Chain Reaction; D011446:Prospective Studies
D004279:DNA, Viral