The structure of the O-specific polysaccharide from Thiobacillus ferrooxidans IFO 14262.
Lipopolysaccharide (LPS) was isolated from Thiobacillus ferrooxidans IFO 14262 by the hot phenol-water extraction procedure. The O-specific polysaccharide, liberated from LPS by mild acetic acid hydrolysis, had a branched pentasaccharide repeating-unit composed of D-glucose, L-rhamnose, D-rhamnose, and 3-O-methyl-L-rhamnose in approximate molar ratios of 2:1:1:1. On the basis of methylation analysis, 1H and 13C NMR spectroscopy, including 2D shift-correlated (COSY) and 1D NOE spectroscopy, the structure for the repeating unit of the O-specific polysaccharide was established, and the assumed biological repeating unit indicated.
Vinogradov
E V
EV
M.M. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Campos-Portuguez
S
S
Yokota
A
A
Mayer
H
H
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002247:Carbon Isotopes; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D013855:Thiobacillus / Q000737:chemistry* / Q000276:immunology
D002241:Carbohydrates; D002247:Carbon Isotopes; D019081:O Antigens; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D006859:Hydrogen