Mepacrine inhibits amylase secretion mediated by a guanylnucleotide binding protein.
1. In intact rat pancreatic acini, 10 nM bombesin, 100 nM phorbol ester TPA and 10 mM fluoroaluminate increased amylase secretion 5-, 3- and 4-fold respectively. 2. Mepacrine dose-dependently inhibited the response to bombesin and fluoroaluminate but did not affect the response to TPA. 3. In permeabilized acini, mepacrine inhibited the secretory response to GTP gamma S without modifying the response to TPA.
Grosfils
K
K
Department of General and Human Biochemistry, Free University of Brussels, Belgium.
Métioui
M
M
Dehaye
J P
JP
D000535:Aluminum / Q000494:pharmacology*; D000681:Amylases / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D001839:Bombesin / Q000494:pharmacology*; D004789:Enzyme Activation; D005461:Fluorine / Q000494:pharmacology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000037:antagonists & inhibitors*; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D010539:Permeability; D011493:Protein Kinase C / Q000187:drug effects; D011796:Quinacrine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012636:Secretory Rate / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
C053629:fluoroaluminum; D005461:Fluorine; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D000535:Aluminum; D011493:Protein Kinase C; D000681:Amylases; D011796:Quinacrine; D013755:Tetradecanoylphorbol Acetate; D001839:Bombesin