mRNA levels and methylation patterns of the 2-5A synthetase gene in control and Alzheimer's disease (AD) fibroblasts.
We have examined the mRNA levels and methylation patterns of the interferon-inducible 2',5'-oligoadenylate (2-5A) synthetase gene in skin fibroblasts derived from AD and age/sex-matched control subjects. Northern or slot hybridization analysis of total RNA showed a 63% and 46% decrease in the steady state level of 2-5A synthetase mRNA in AD cells as compared to controls, following a 24 h and 48 h treatment with IFN-beta ser. The 2-5A synthetase gene as studied by Southern analysis using the methylation-sensitive restriction enzymes HpaII and Msp I was found to be hypomethylated in AD cells. No difference in methylation patterns of the actin gene existed between control and AD fibroblasts.
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Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla 10595.
Khanna
K K
KK
Wu
J M
JM
D015088:2',5'-Oligoadenylate Synthetase / Q000235:genetics*; D000367:Age Factors; D000544:Alzheimer Disease / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology; D016555:Matched-Pair Analysis; D008745:Methylation; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012737:Sex Factors; D012867:Skin / Q000473:pathology
D012333:RNA, Messenger; D016899:Interferon-beta; D004247:DNA; D015088:2',5'-Oligoadenylate Synthetase; D019003:Deoxyribonuclease HpaII; D015252:Deoxyribonucleases, Type II Site-Specific