Distribution of dopamine-immunoreactive neurons and their relationships to transmitter and hypothalamic hormone-immunoreactive neuronal systems in the rat mediobasal hypothalamus. A morphometric and microdensitometric analysis.
A morphometric and microdensitometric characterization of the dopamine neurons of the mediobasal hypothalamus and their relationships with several other chemically identified systems, including putative tyrosine hydroxylase-positive/dopamine-negative neurons, was carried out after visualization of dopamine content by both immunocytochemistry and the Falck-Hillarp technique. Quantitative assessment of co-existence demonstrated that more than 95% of dopamine-immunoreactive neurons also contained tyrosine hydroxylase immunoreactivity and more than 90% of growth hormone-releasing factor-immunoreactive neurons also contained tyrosine hydroxylase immunoreactivity. Morphometric and densitometric analysis of dopamine, tyrosine hydroxylase and growth hormone-releasing factor-immunoreactive neurons in the arcuate nucleus showed that dopamine/tyrosine hydroxylase-containing and growth hormone-releasing factor/tyrosine hydroxylase-containing neuronal populations are two largely segregated cell groups with specific localization in the arcuate region, rostrocaudal extension and tyrosine hydroxylase-immunoreactivity content. Morphometric characteristics of dopamine-immunoreactive neurons were shown to be equivalent to those of catecholamine fluorescent cell bodies in the arcuate region. In addition, a cell group lacking detectable catecholamine fluorescence in normal animals but accumulating L-DOPA after peripheral loading was identified and characterized from a morphometric standpoint in the ventral premammillary nucleus. Quantitative analysis of nerve terminal co-distribution in the median eminence revealed significant correlations between dopamine and other transmitter or neurohormone systems, such as gamma-aminobutyric acid, galanin, luteinizing hormone-releasing hormone, in specific subregions of the palissade zone. These data point to discrete subregions of the median eminence, which have been called 'medianosomes', as main sites of interactions between transmitter-identified nerve terminal systems in the control of hypothalamic hormone release.
Zoli
M
M
Institute of Human Physiology, University of Modena, Italy.
Agnati
L F
LF
Tinner
B
B
Steinbusch
H W
HW
Fuxe
K
K
D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000737:chemistry* / Q000473:pathology; D002395:Catecholamines / Q000032:analysis; D003720:Densitometry; D004298:Dopamine / Q000032:analysis*; D019004:Galanin; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis*; D013007:Growth Hormone-Releasing Hormone / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008297:Male; D008846:Micromanipulation; D009474:Neurons / Q000737:chemistry* / Q000473:pathology; D009479:Neuropeptides / Q000032:analysis; D018377:Neurotransmitter Agents / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis
D002395:Catecholamines; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D010455:Peptides; D007987:Gonadotropin-Releasing Hormone; D019004:Galanin; D013007:Growth Hormone-Releasing Hormone; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine