Evaluation of monoclonal anti-glycophorin B as an unusual anti-S.
BACKGROUND
Monoclonal antibody 148 is a murine monoclonal anti-glycophorin B that preferentially reacts with S+ human red cells.


METHODS
Serologic and immunochemical studies were performed using red cells with various phenotypes.


RESULTS
These studies reveal that this monoclonal antibody is unusual in that it fails to agglutinate S+ TSEN+ red cells and agglutinates S- St(a+) and S- Dantu+ red cells.


CONCLUSIONS
These results allow the prediction of the glycophorin composition of GP.Hop (Mi.IV) red cells.
Hemming
N J
NJ
International Blood Group Reference Laboratory, SW Regional Transfusion Centre, Bristol, UK.
Reid
M E
ME
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000939:Epitopes; D004910:Erythrocyte Membrane / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D006021:Glycophorins / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D015151:Immunoblotting; D007519:Isoantigens / Q000276:immunology*; D008951:MNSs Blood-Group System / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006021:Glycophorins; D007519:Isoantigens; D008951:MNSs Blood-Group System