title: Hemagglutination activity of Lactobacillus acidophilus group lactic acid bacteria.
abstract: The cells of 28 strains of the Lactobacillus acidophilus group were evaluated for hemagglutination (HA) activity. The activity was found in the surface layer (SL) protein fraction extracted by 2 M guanidine hydrochloride. The most SL proteins from the A group strains (L. acidophilus (A1), L. crispatus (A2), L. amylovorus (A3), and L. gallinarum (A4)) showed HA activity, but the proteins from the B group strains (L. gasseri (B1) and L. johnsonii (B2)) showed no activity. The SL proteins from the A group strains were composed in common of a main component having molecular mass of about 40-45 kDa on SDS-PAGE. The SL proteins from JCM 1034 strain that showed the highest HA activity was fractionated by CM-Toyopearl ion-exchange chromatography. The highest HA activity was detected in the major protein of 41 kDa. This protein was purified and shown to be composed of about 50% of hydrophobic amino acids. The HA activity of the protein (1034 lectin) was specifically inhibited by fetuin and bovine lactoferrin at the concentrations of 80 and 160 micrograms/ml, respectively. The removal of N-acetylneuraminic acid from fetuin significantly decreased the inhibitory activity.
authors.affiliation: Laboratory of Animal Products Chemistry, College of Agriculture, Tohoku University, Sendai, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: T
authors.initials: T
authors.lastname: Toba
authors.forename: H
authors.initials: H
authors.lastname: Kitazawa
authors.forename: J
authors.initials: J
authors.lastname: Uemura
authors.forename: T
authors.initials: T
authors.lastname: Itoh
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000276:immunology; D001665:Binding Sites; D001709:Biotechnology; D002240:Carbohydrate Sequence; D002364:Caseins / Q000737:chemistry / Q000378:metabolism; D006020:Glycopeptides / Q000737:chemistry / Q000378:metabolism; D006384:Hemagglutination; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D066298:In Vitro Techniques; D007779:Lactobacillus acidophilus / Q000737:chemistry / Q000145:classification / Q000276:immunology*; D007781:Lactoferrin / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry / Q000494:pharmacology; D013045:Species Specificity; D000509:alpha-Fetoproteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D002364:Caseins; D006020:Glycopeptides; D006388:Hemagglutinins; D009844:Oligosaccharides; D000509:alpha-Fetoproteins; D007781:Lactoferrin