Rapid anterograde axonal transport of the cellular prion glycoprotein in the peripheral and central nervous systems.
In prion diseases, the cellular prion protein (PrPc), abundant in neurons, is converted posttranslationally into an amyloid-forming scrapie prion protein (PrPSc), which accumulates in white matter tracts and nerve terminals. The trafficking of PrPc in neurons was investigated in vivo by injecting [35S]methionine into the L4 and L5 dorsal root ganglia and the entorhinal cortices of adult rats and by tracing the movement of radiolabeled PrPc. In both paradigms, labeled 33-35-kDa PrPc was transported, within 4 h, to distal axons and nerve terminals cofractionating with proteins in the fast component. Future studies using these methods may allow us to determine whether PrPc is converted into PrpSc during axonal transport and whether PrPSc is transported in animals with prion diseases.
Borchelt
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Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2196.
Koliatsos
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Guarnieri
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Pardo
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Sisodia
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Price
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D000818:Animals; D001370:Axonal Transport; D001921:Brain / Q000378:metabolism / Q000502:physiology*; D002460:Cell Line; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005727:Ganglia, Spinal / Q000378:metabolism / Q000502:physiology*; D008647:Mesocricetus; D008715:Methionine / Q000378:metabolism; D009411:Nerve Endings / Q000378:metabolism / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D009928:Organ Specificity; D018620:PrPSc Proteins; D011328:Prions / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits / Q000276:immunology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000378:metabolism / Q000502:physiology*; D013462:Sulfur Radioisotopes
D009419:Nerve Tissue Proteins; D018620:PrPSc Proteins; D011328:Prions; D013462:Sulfur Radioisotopes; D008715:Methionine