Studies of the structure and binding properties of hamster female protein.
We report here the characterization of hamster female protein (FP), a member of the pentraxin family of plasma proteins, as a molecule composed of glycosylated subunits of 25,655 MW containing a single intrachain disulphide bridge. In the presence of EDTA the subunits are non-covalently associated as pentamers of mass approximately 128,000 MW, and in the presence of calcium they aggregate further, probably to form decamers. This pentamer-decamer transition at physiological ionic strength has not been described in other pentraxins. As previously reported, FP shares the capacity of C-reactive protein (CRP) in other species to bind phosphocholine and we show here that it also resembles human CRP in binding only weakly to agarose, to human AA amyloid fibrils in vitro, and to mouse AA amyloid deposits in vivo. It thus differs markedly from human and mouse serum amyloid P component (SAP) but it is nevertheless deposited in hamster AA amyloid in vivo and clearly is the hamster counterpart of SAP in other species. These results illustrate the subtle diversity among members of the otherwise conserved pentraxin family of vertebrate plasma proteins.
Tennent
G A
GA
Department of Medicine, Royal Postgraduate Medical School, London, U.K.
Baltz
M L
ML
Osborn
G D
GD
Butler
P J
PJ
Noble
G E
GE
Hawkins
P N
PN
Pepys
M B
MB
D000510:Alpha-Globulins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D000686:Amyloidosis / Q000378:metabolism; D000818:Animals; D002097:C-Reactive Protein; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D008647:Mesocricetus / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D000685:Serum Amyloid A Protein / Q000378:metabolism; D000683:Serum Amyloid P-Component / Q000378:metabolism
D000510:Alpha-Globulins; D000685:Serum Amyloid A Protein; D000683:Serum Amyloid P-Component; C030974:female protein, hamster; D002097:C-Reactive Protein