A reduced level of multiple mutation in a shuttle vector passaged in Sjögren's syndrome cells.
Sjögren's syndrome is a systemic disorder with unknown etiology, displaying many signs of autoimmunity. Although the basic mechanism of this disease is unknown, a defect in somatic mutagenesis of antibody genes has been suggested. Using a shuttle vector plasmid, we here show that the number of vectors with multiple base changes in a marker gene was reduced in B cell lines from two patients with Sjögren's syndrome (8% in both), as compared with values reported for cell lines from normal human donors (16-27%). This finding suggests that a reduction of the rate of somatic mutagenesis may influence the development of symptoms in Sjögren patients.
Henriksson
G
G
Department of Medical Microbiology, Lund University, Malmö General Hospital, Sweden.
Sandor
Z
Z
Aponyi
I
I
Manthorpe
R
R
Bredberg
A
A
D001402:B-Lymphocytes / Q000528:radiation effects; D001483:Base Sequence; D002478:Cells, Cultured; D004252:DNA Mutational Analysis; D004260:DNA Repair; D004279:DNA, Viral / Q000235:genetics; D018274:Electroporation; D005798:Genes, Bacterial / Q000235:genetics; D005802:Genes, MHC Class II / Q000235:genetics* / Q000276:immunology; D016153:Genes, Suppressor / Q000235:genetics; D005819:Genetic Markers; D005822:Genetic Vectors / Q000235:genetics / Q000528:radiation effects; D006801:Humans; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids / Q000528:radiation effects; D017354:Point Mutation; D011836:Radiation Tolerance; D012859:Sjogren's Syndrome / Q000235:genetics*; D014466:Ultraviolet Rays
D004279:DNA, Viral; D005819:Genetic Markers