title: Polymorphism in the human E-selectin gene detected by PCR-SSCP.
abstract: By using the non-isotopic single-strand conformation polymorphism (SSCP) technique to analyse products of the polymerase chain reaction (PCR), we detected a 561-adenine to cytosine substitution resulting in an amino acid exchange from serine to arginine at position 128 of the E-selectin gene. If this amino acid substitution has an effect on the adhesion of blood cells to the endothelium, the polymorphism could be of interest with respect to association studies in a number of pathological conditions, such as cardiovascular diseases.
authors.affiliation: Medizinische Klinik, Universitätsklinikum Charité, Humboldt-Universität, Berlin, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Wenzel
authors.forename: R
authors.initials: R
authors.lastname: Hanke
authors.forename: A
authors.initials: A
authors.lastname: Speer
mesh_terms: D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000235:genetics*; D019040:E-Selectin; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D018807:Polymorphism, Single-Stranded Conformational
chemical_list: D015815:Cell Adhesion Molecules; D019040:E-Selectin