title: Arabidopsis assay for mutagenicity.
abstract: Four laboratories, two in the Czech Republic (Brno and Prague) and two in the CIS (Moscow and Duschanbe), participated in the International Programme on Chemical Safety's (IPCS) collaborative study to evaluate the utility of the most commonly used plant test systems, including the Arabidopsis thaliana assay, for assessing the mutagenic potential of environmental agents. Out of the five compounds evaluated in the Arabidopsis assay, three compounds, i.e., ethyl methanesulfonate, N-methyl-N-nitrosourea, and azidoglycerol, were reported to be mutagenic by all four participating laboratories. Sodium azide (NaN3) demonstrated a negative response in all four laboratories, whereas maleic hydrazide was reported to be weakly mutagenic by one laboratory and nonmutagenic by the other three laboratories.
authors.affiliation: Institute of Experimental Botony, Prague, Czech Republic.
authors.forename: T
authors.initials: T
authors.lastname: Gichner
authors.forename: S A
authors.initials: SA
authors.lastname: Badayev
authors.forename: S I
authors.initials: SI
authors.lastname: Demchenko
authors.forename: J
authors.initials: J
authors.lastname: Relichová
authors.forename: S S
authors.initials: SS
authors.lastname: Sandhu
authors.forename: P D
authors.initials: PD
authors.lastname: Usmanov
authors.forename: O
authors.initials: O
authors.lastname: Usmanova
authors.forename: J
authors.initials: J
authors.lastname: Velemínský
mesh_terms: D017360:Arabidopsis / Q000196:embryology / Q000235:genetics*; D001386:Azides / Q000633:toxicity; D001681:Biological Assay / Q000379:methods; D004784:Environmental Monitoring / Q000379:methods; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D007391:International Cooperation; D008300:Maleic Hydrazide / Q000633:toxicity; D008770:Methylnitrosourea / Q000633:toxicity; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000032:analysis / Q000633:toxicity; D011409:Propylene Glycols / Q000633:toxicity; D015203:Reproducibility of Results; D019810:Sodium Azide
chemical_list: D001386:Azides; D009153:Mutagens; D011409:Propylene Glycols; D008770:Methylnitrosourea; C052086:azidoglycerol; D019810:Sodium Azide; D005020:Ethyl Methanesulfonate; D008300:Maleic Hydrazide