title: [Study of the structural organization of OSCP--subunits of H+-ATPase of porcine heart mitochondria].
abstract: Unmodified and citraconilated OSCP of the pig heart mitochondrial H(+)-ATPase were hydrolysed by proteinase from Staphylococcus aureus V8 and trypsin, respectively. To purify the individual peptides, various types of HPLC and covalent chromatography on SH-Sepharose were used. By the automatic Edman method complete or partial amino acid sequences of the peptides obtained were determined, thus allowing for the reconstruction of the primary structure of pig OSCP. A linear antigenic determinant recognizable by A1 monoclonal antibody against bovine OSCP, was localized. Studies showed Gly43 residue (bovine OSCP) to be replaced by Ala43 (pig OSCP), which is responsible for a decrease of the affinity of the monoclonal antibody A1 to pig OSCP. Comparative analysis of primary structures of bovine and pig OSCP was carried out.
authors.forename: V A
authors.initials: VA
authors.lastname: Grinkevich
authors.forename: V G
authors.initials: VG
authors.lastname: Zaĭtsev
authors.forename: P F
authors.initials: PF
authors.lastname: Pavlov
authors.forename: I V
authors.initials: IV
authors.lastname: Nazimov
authors.forename: E F
authors.initials: EF
authors.lastname: Il'ina
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins; D002417:Cattle; D000939:Epitopes / Q000032:analysis; D008565:Membrane Proteins / Q000737:chemistry*; D008929:Mitochondria, Heart / Q000201:enzymology*; D025261:Mitochondrial Proton-Translocating ATPases; D008969:Molecular Sequence Data; D009840:Oligomycins / Q000494:pharmacology; D011487:Protein Conformation; D006180:Proton-Translocating ATPases / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D013552:Swine
chemical_list: D002352:Carrier Proteins; D000939:Epitopes; D008565:Membrane Proteins; D009840:Oligomycins; D000251:Adenosine Triphosphatases; D025261:Mitochondrial Proton-Translocating ATPases; D006180:Proton-Translocating ATPases; C020439:oligomycin sensitivity-conferring protein