Synergistic stimulation of human B lymphocytes by anti-CD40 monoclonal antibodies and synthetic lipopeptide analogues from Escherichia coli lipoprotein.
Human tonsillar B lymphocytes were stimulated with synthetic lipopeptide analogues of Escherichia coli lipoprotein alone or together with anti-CD40 and/or interleukin-4 (IL-4). While lipopeptides alone or lipopeptides plus IL-4 did not include proliferation of B lymphocytes, synergistic stimulation was observed when anti-CD40 antibodies were added. Proliferation was even more pronounced in the presence of Fc receptor type II (FcRII)-transfected L cells. Compared to the stimulus anti-CD40 plus IL-4 plus FcRII-transfected fibroblasts exerted, the addition of lipopeptides induced a more rapid maximal response which peaked on day 4. Antibody production could also be enhanced by lipopeptides. Our data provide evidence that lipopeptides, which act as mitogens toward murine B lymphocytes, also stimulate human B lymphocytes, provided that co-signals are added.
Edinger
M
M
Institut für Immunbiologie, Universität Freiburg, Germany.
Bessler
W G
WG
Kleine
B
B
D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology; D019013:CD40 Antigens; D002478:Cells, Cultured; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D015847:Interleukin-4 / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008074:Lipoproteins / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D014066:Palatine Tonsil / Q000276:immunology; D010455:Peptides / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; D001426:Bacterial Proteins; D019013:CD40 Antigens; D008070:Lipopolysaccharides; D008074:Lipoproteins; D010455:Peptides; D015847:Interleukin-4