Triton channels are sensitive to divalent cations and protons.
Addition of Triton X-100 to planar bilayers composed of dioleoyl phosphatidyl choline, diphytanoyl phosphatidyl choline or mono-oleoyl glycerol induces single channel-like events when electrical conductivity across the bilayer is measured. Addition of divalent cations or protons causes channels to disappear; single channel conductance of remaining channels is not significantly altered; addition of EDTA or alkali (respectively) reverses the effect. It is concluded that sensitivity to divalent cations and protons need not be dependent on specific channel proteins or pore-forming toxins, but may be a feature of any aqueous pore across a lipid milieu.
Rostovtseva
T K
TK
Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, London, United Kingdom.
Bashford
C L
CL
Lev
A A
AA
Pasternak
C A
CA
D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology; D007473:Ion Channels / Q000737:chemistry* / Q000187:drug effects; D008567:Membranes, Artificial; D017830:Octoxynol / Q000494:pharmacology*; D011522:Protons
D002413:Cations, Divalent; D007473:Ion Channels; D008567:Membranes, Artificial; D011522:Protons; D017830:Octoxynol; D002118:Calcium