Immunomagnetic positive selection and colony culture of CD34+ cells from blood.
Immunomagnetic separation has been used to enrich CD34-positive cells in umbilical cord blood. Cell purities were increased from 0.59% preseparation to 92.7% postseparation (n = 16) with a mean yield of 75.7%. CFU were enriched 127 fold by immunomagnetic separation. Addition of combinations of recombinant growth factors resulted in cloning efficiencies of greater than 50%.
Law
P
P
Baxter Healthcare Corporation, Immunotherapy Division, Santa Ana, CA 92705.
Ishizawa
L
L
van de Ven
C
C
Burgess
J
J
Hardwick
A
A
Plunkett
M
M
Gee
A P
AP
Cairo
M
M
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018952:Antigens, CD34; D002452:Cell Count; D003114:Colony-Forming Units Assay; D005312:Fetal Blood / Q000166:cytology*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation; D016130:Immunophenotyping; D007231:Infant, Newborn; D019089:Stem Cell Factor
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018952:Antigens, CD34; D016298:Hematopoietic Cell Growth Factors; D019089:Stem Cell Factor