Structural studies of the O-polysaccharide from the lipopolysaccharide of Moraxella (Branhamella) catarrhalis serotype A (strain ATCC 25238).
The polysaccharide of the Moraxella (Branhamella) catarrhalis serotype A lipopolysaccharide was prepared by mild acid hydrolysis followed by gel permeation chromatography. The structure was established by methylation analysis, mass spectrometry, and NMR spectroscopy. It is concluded that the O-antigenic polysaccharide has the following structure. [formula see text] Methylation analysis of the intact lipopolysaccharide showed that the lipid A portion consisted of 6-substituted glucosamine residues. Methylation followed by methanolysis showed that two Kdo residues were present, one terminal and one 4,5-substituted residue. A terminal Kdo thus substitutes the branch-point Kdo in the 4-position.
Edebrink
P
P
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
Jansson
P E
PE
Rahman
M M
MM
Widmalm
G
G
Holme
T
T
Rahman
M
M
Weintraub
A
A
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D005944:Glucosamine / Q000032:analysis; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D001936:Moraxella catarrhalis / Q000737:chemistry* / Q000145:classification / Q000276:immunology; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D012703:Serotyping; D013400:Sugar Acids / Q000032:analysis
D007202:Indicators and Reagents; D019081:O Antigens; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; D005944:Glucosamine