Release of histamine from isolated rat hearts during reperfusion is not dependent on length of ischemic insult, or contents of histamine in cardiac tissue.
Release of histamine (H) by ischemia-reperfusion injury was investigated in isolated rat hearts (Langendorff model). The effect of 10, 15, 20, 25, 30, 40 and 60 min ischemia (n = 10 each) on H in the coronary effluent and in cardiac tissue was studied after 4 min reperfusion. Release of creatine kinase and lactate dehydrogenase in the coronary effluent increased with time of ischemia. Tissue H increased from 95 +/- 10 ng/g rat heart (mean +/- SEM) before ischemia to max 148 +/- 10 ng/g after 20 min ischemia (p < 0.002), and increased also after 15 (p < 0.01), 25 (p < 0.01), and 30 min (p < 0.045). H in the coronary effluent increased after 15 (from 16 +/- 3 to 26 +/- 2 pmol/min, p < 0.044), 30 (26 +/- 6 pmol/min, p < 0.027), and 60 min ischemia (47 +/- 6 pmol/min, p < 0.0044). Release of H during ischemia-reperfusion is neither dependent on the severity of the ischemic insult, nor on the level of tissue H.
Valen
G
G
Department of Thoracic Surgery, Karolinska Hospital, Stockholm, Sweden.
Kaszaki
J
J
Szabo
I
I
Nagy
S
S
Vaage
J
J
D000818:Animals; D015415:Biomarkers; D003402:Creatine Kinase / Q000378:metabolism; D006339:Heart Rate / Q000502:physiology; D006439:Hemodynamics / Q000502:physiology; D006632:Histamine / Q000378:metabolism*; D006636:Histamine Release / Q000502:physiology*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D017202:Myocardial Ischemia / Q000378:metabolism* / Q000503:physiopathology; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D017725:Ventricular Pressure / Q000502:physiology
D015415:Biomarkers; D006632:Histamine; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase