title: Inactivation of bleomycin by an N-acetyltransferase in the bleomycin-producing strain Streptomyces verticillus.
abstract: Bleomycin-producing Streptomyces verticillus ATCC15003 possesses a bleomycin acetyltransferase which inactivates the drug in the presence of acetyl coenzyme A. The site of acylation in enzymically prepared acetylbleomycin A2 was determined by nuclear magnetic resonance analysis; the primary amino group of the beta-aminoalanine moiety of bleomycin was acetylated Acetylbleomycin A2 had no detectable antibacterial activity and did not induce in vitro DNA degradation.
authors.affiliation: Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sugiyama
authors.forename: T
authors.initials: T
authors.lastname: Kumagai
authors.forename: M
authors.initials: M
authors.lastname: Shionoya
authors.forename: E
authors.initials: E
authors.lastname: Kimura
authors.forename: J E
authors.initials: JE
authors.lastname: Davies
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D001761:Bleomycin / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology* / Q000031:analogs & derivatives; D009682:Magnetic Resonance Spectroscopy; D013302:Streptomyces / Q000378:metabolism*
chemical_list: C026658:acetylbleomycin A2; D001761:Bleomycin; D000123:Acetyltransferases