Nucleotide sequence and expression of a ripening and water stress-related cDNA from tomato with homology to the MIP class of membrane channel proteins.
The nucleotide sequence and derived amino acid sequence were determined for a full-length version of the tomato cDNA clone, pTOM75, the mRNA for which has previously been shown to accumulate in roots, ripening fruit and senescing leaves. Computer analysis of the predicted protein product, which we have named tomato ripening-associated membrane protein (TRAMP) indicates strong homology to known transmembrane channel proteins from other organisms. Northern analysis showed that this gene was induced by waterstress and that this induction was unaffected in an ABA-deficient genetic background.
Fray
R G
RG
AFRC Research Group in Plant Gene Regulation, University of Nottingham, Loughborough, UK.
Wallace
A
A
Grierson
D
D
Lycett
G W
GW
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D020104:Immunophilins; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D019696:Peptidylprolyl Isomerase; D010940:Plant Proteins; D012313:RNA / Q000032:analysis / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D014675:Vegetables / Q000235:genetics*
D001426:Bacterial Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D010940:Plant Proteins; C086265:TRAMP protein, Lycopersicon esculentum; D012313:RNA; D020104:Immunophilins; D019696:Peptidylprolyl Isomerase