Antisense effects of cholesterol-oligodeoxynucleotide conjugates associated with poly(alkylcyanoacrylate) nanoparticles.
Oligonucleotides covalently attached to a cholesteryl moiety are more stable in biological media and better taken up by eukaryotic cells. However, their anchoring in hydrophobic cellular membranes and endosomes after endocytosis restricts their access to cellular nucleic acids. New methods of cellular delivery and the biological activity of the conjugates were studied. The cholesteryl residue was conjugated via disulfide bond to the 5' or 3' terminal phosphate group of two oligodeoxyribonucleotide dodecamers complementary to the mutated region of Ha-ras oncogene mRNA. The conjugates were able to form complementary duplexes with the mutated 27-b target fragment of mRNA but not with the wild-type sequence. Efficient sequence-specific RNase H cleavage of complementary mRNA was induced with low (< or = 500 nM) concentrations of the conjugates. At higher concentrations, this cleavage was progressively inhibited, probably due to an interaction between RNase H and the cholesterol residue. The hydrophobic conjugates could be adsorbed onto poly(isohexylcyanoacrylate) nanoparticles via their cholesteryl moieties and delivered to eukaryotic cells. Cholesterol-conjugated oligonucleotides were able to sequence-specifically inhibit the proliferation of T24 human bladder carcinoma cells in culture.
Godard
G
G
Laboratoire de Biophysique, INSERM U201, CNRS URA481, Muséum National d'Histoire Naturelle, Paris, France.
Boutorine
A S
AS
Saison-Behmoaras
E
E
Hélène
C
C
D000327:Adsorption; D001483:Base Sequence; D001665:Binding Sites; D002784:Cholesterol / Q000008:administration & dosage* / Q000378:metabolism; D003487:Cyanoacrylates; D016503:Drug Delivery Systems; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D010316:Particle Size; D014677:Pharmaceutical Vehicles; D011108:Polymers; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016914:Ribonuclease H; D014407:Tumor Cells, Cultured
D003487:Cyanoacrylates; D016376:Oligonucleotides, Antisense; D014677:Pharmaceutical Vehicles; D011108:Polymers; D012333:RNA, Messenger; D002784:Cholesterol; D016914:Ribonuclease H