Dielectrophoretic separation and enrichment of CD34+ cell subpopulation from bone marrow and peripheral blood stem cells.
Dielectrophoresis has been used to enrich selected cell subpopulations in a mixed cell population by exploiting differential dielectric properties. Six-fold enrichment of stem cells expressing the CD34+ antigen has been achieved for bone marrow samples and peripheral blood, without the requirement for initial chemical treatment associated with immunoadsorption techniques.
Talary
M S
MS
Institute of Molecular and Biomolecular Electronics, University of Wales, Gwynedd, UK.
Mills
K I
KI
Hoy
T
T
Burnett
A K
AK
Pethig
R
R
D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D001854:Bone Marrow Cells; D002469:Cell Separation / Q000379:methods*; D004586:Electrophoresis / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans
D015703:Antigens, CD; D018952:Antigens, CD34