Gene PSO5 of Saccharomyces cerevisiae, involved in repair of oxidative DNA damage, is allelic to RAD16.
The pos5-1 mutation renders Saccharomyces cerevisiae cells sensitive to DNA-damaging agents. We have isolated plasmids from a S. cerevisiae genomic library capable of restoring wild-type levels of 254-nm ultraviolet light sensitivity of the pso5-1 mutant. DNA sequence analysis revealed that the complementing activity resides in RAD16, a gene involved in excision repair. Tetrad analysis showed that PSO5, like RAD16, is tightly linked to LYS2 on chromosome II. Moreover, allelism between the pso5-1 and rad16 mutants was demonstrated by the comparison of mutagen sensitivity phenotypes, complementation tests, and by meiotic analysis. The cloned RAD16 gene was capable of restoring wild-type resistance of the pso5-1 mutant to H2O2 and photoactivated 3-carbethoxypsoralen, both treatments generating oxidative stress-related DNA damage. This indicates that RAD16/PSO5 might also participate in the repair of oxidative base damage.
Paesi-Toresan
S O
SO
Departamento de Biofísica, Universidade Federal do Rio Grando do Sul, Porto Alegre, Brasil.
Pich
C T
CT
Grey
M
M
Keszenman-Pereyra
D
D
Brendel
M
M
Henriques
J A
JA
D000483:Alleles; D004249:DNA Damage; D004260:DNA Repair / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D010084:Oxidation-Reduction; D011836:Radiation Tolerance / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays
D004271:DNA, Fungal