Functional characterization of rat chemokine macrophage inflammatory protein-2.
Expression of mRNA for the C-X-C chemokine, macrophage inflammatory protein-2 (MIP-2), is induced during acute inflammation in rat models of disease. We have characterized the phlogistic potential of rat recombinant MIP-2 (rMIP-2) protein in vitro and in vivo. Recombinant MIP-2 caused marked PMN chemotaxis in vitro, with peak chemotactic activity at 10 nM. Incubation of whole blood with rMIP-2 caused a significant loss of L-selectin and a significant increase in Mac-1 expression on the PMN surface. Under similar conditions rMIP-2 also caused a modest respiratory burst in PMNs. The intratracheal instillation of 10 and 50 micrograms of rMIP-2 caused a significant influx of PMNs into the airspace of the lungs. Rat MIP-2 is a potent neutrophil chemotactic factor capable of causing neutrophil activation and is likely to function in PMN recruitment during acute inflammation in rat disease models.
Frevert
C W
CW
Physiology Program, Harvard School of Public Health, Boston, Massachusetts.
Farone
A
A
Danaee
H
H
Paulauskis
J D
JD
Kobzik
L
L
D000818:Animals; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology; D002478:Cells, Cultured; D054426:Chemokine CXCL2; D002630:Chemotactic Factors / Q000502:physiology; D016207:Cytokines / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D019041:L-Selectin; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D008969:Molecular Sequence Data; D015846:Monokines / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology; D015345:Oligonucleotide Probes / Q000235:genetics; D051381:Rats; D016897:Respiratory Burst
D015815:Cell Adhesion Molecules; D054426:Chemokine CXCL2; D002630:Chemotactic Factors; D016207:Cytokines; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; D015846:Monokines; D015345:Oligonucleotide Probes; D019041:L-Selectin