title: Intrinsic transcript cleavage activity of RNA polymerase.
abstract: The GreA and GreB transcript cleavage factors of Escherichia coli suppress elongation arrest and may have a proofreading role in transcription. With the use of E. coli greA-greB- mutant, RNA polymerase is demonstrated to possess substantial intrinsic transcript cleavage activity. Mildly alkaline pH mimics the effect of the Gre proteins by inducing transcript cleavage in ternary complexes and antagonizing elongation arrest through a cleavage-and-restart reaction. Thus, transcript cleavage constitutes the second enzymological activity of RNA polymerase along with polymerization/pyrophosphorolysis of RNA, whereas the Gre proteins merely enhance this intrinsic property.
authors.affiliation: Public Health Research Institute, New York, NY 10016, USA.
authors.forename: M
authors.initials: M
authors.lastname: Orlova
authors.forename: J
authors.initials: J
authors.lastname: Newlands
authors.forename: A
authors.initials: A
authors.lastname: Das
authors.forename: A
authors.initials: A
authors.lastname: Goldfarb
authors.forename: S
authors.initials: S
authors.lastname: Borukhov
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D017353:Gene Deletion; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010445:Peptide Elongation Factors / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000096:biosynthesis*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D035602:Transcriptional Elongation Factors
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C079667:GreB protein, E coli; D009838:Oligodeoxyribonucleotides; D010445:Peptide Elongation Factors; D012329:RNA, Bacterial; D014157:Transcription Factors; D035602:Transcriptional Elongation Factors; C075886:GREA protein, Rickettsia prowazekii; D012321:DNA-Directed RNA Polymerases