title: Molecular fingerprinting of Pasteurella multocida associated with progressive atrophic rhinitis in swine herds.
abstract: Ninety-six nasal isolates of Pasteurella multocida from swine herds with progressive atrophic rhinitis were characterized by restriction endonuclease analysis (REA) of whole-cell DNA, ribotyping, and plasmid analysis. For REA, bacterial DNA was digested with SmaI and electrophoresed in 0.7% agarose, and fragments were visualized with UV light. For ribotyping, EcoRI-digested and electrophoresed restriction fragments of whole-cell DNA were transferred to nitrocellulose membranes, hybridized with gamma-32P-labeled Escherichia coli ribosomal RNA, and visualized by autoradiography. Phenotypes of isolates were toxigenic capsular type D (n = 51), nontoxigenic type D (n = 28), nontoxigenic type A (n = 16), and toxigenic type A (n = 1). Plasmids of various sizes were evident in 92.2% and 17.9% of toxigenic and nontoxigenic D strains, respectively, but were absent from all type A strains. Among the 4 phenotypes, there were 17 REA profiles and 6 ribotypes. For 3 of 17 REA patterns, multiple ribotypes were evident, and several REA types were evident in 5 of 6 ribotypes. Thirty-seven isolates of toxigenic capsular type D from Australian herds were either SmaI type B or C and ribotype 2, whereas 14 toxigenic D isolates from the USA and other countries were more heterogeneous (7 REA types and 6 ribotypes). The fingerprinting results provided evidence in support of the hypothesis of a single source infection in Australia associated with the introduction of breeding pigs from overseas.
authors.affiliation: Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis 95616.
authors.forename: I A
authors.initials: IA
authors.lastname: Gardner
authors.forename: R
authors.initials: R
authors.lastname: Kasten
authors.forename: G J
authors.initials: GJ
authors.lastname: Eamens
authors.forename: K P
authors.initials: KP
authors.lastname: Snipes
authors.forename: R J
authors.initials: RJ
authors.lastname: Anderson
mesh_terms: D000818:Animals; D001315:Australia; D016667:Bacterial Capsules / Q000145:classification; D015139:Blotting, Southern / Q000662:veterinary; D016172:DNA Fingerprinting / Q000662:veterinary; D004262:DNA Restriction Enzymes; D004196:Disease Outbreaks / Q000662:veterinary; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D010326:Pasteurella Infections / Q000382:microbiology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000235:genetics*; D012329:RNA, Bacterial / Q000145:classification; D012222:Rhinitis, Atrophic / Q000382:microbiology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D012329:RNA, Bacterial; D004262:DNA Restriction Enzymes