In vivo platelet deposition on polytetrafluoroethylene coated with fibrin glue containing fibroblast growth factor 1 and heparin in a canine model.
BACKGROUND
We previously reported that the coating of expanded polytetrafluoroethylene (ePTFE) with fibrin glue containing fibroblast growth factor 1 (FGF-1) and heparin accelerates endothelial coverage of grafts implanted into animals. We report here the effect of this surface modification on early platelet deposition.


METHODS
Nine dogs received 7-cm ePTFE grafts, 60-microns internodal distance, 4-mm internal diameter, as bilateral aortoiliac implants, one coated (luminal cross section and abluminal surface) with fibrin glue (fibrinogen 32.1 mg/mliters, thrombin 0.32 U/mliters) containing FGF-1 (11 ng/mliters and heparin (250 U/mliters), the other uncoated. After 5, 30, or 120 minutes of circulation with blood containing autologous platelets radiolabelled with indium 111, gamma emissions were quantitated on explants and correlated to surface areas measured by computerized planimetry.


RESULTS
Both global and segmental comparisons showed significantly (P < 0.05, Student's t-test) less platelet deposition on coated than on uncoated grafts after 120 minutes of circulation, but no difference at 5 and 30 minutes.


CONCLUSIONS
In this model, ePTFE coating with fibrin glue containing FGF-1 and heparin shows no adverse effect on early platelet deposition.
Gosselin
C
C
Department of Surgery, Loyola University Medical Center, Maywood, Illinois.
Ren
D
D
Ellinger
J
J
Greisler
H P
HP
D000818:Animals; D001807:Blood Vessel Prosthesis; D004285:Dogs; D015718:Fibrin Tissue Adhesive; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011138:Polytetrafluoroethylene
D015718:Fibrin Tissue Adhesive; D016220:Fibroblast Growth Factor 1; D011138:Polytetrafluoroethylene; D006493:Heparin