Characterization of the 5' region of the CD10/neutral endopeptidase 24.11 gene.
We have characterized the 5' region of the CALLA/CD10 gene which has been shown to be identical to the membrane-associated enzyme neutral endopeptidase 24.11 (NEP). There is no CAAT or TATA box in the 5' flanking region, upstream of exon 1, but a GC rich region with several Sp1 binding sites. We have detected several putative initiation transcription sites by primer extension and by nuclease S1 analysis. Moreover by reverse transcriptase-polymerase chain reaction, we demonstrated the existence of a new exon: exon 1bis. This exon can be alternatively spliced as has already been described for exon 1 and exon 2.
Haouas
H
H
INSERM U268, Hôpital Paul Brousse, Villejuif, Paris, France.
Morello
D
D
Lavenu
A
A
Billard
M
M
Jasmin
C
C
Boucheix
C
C
D001483:Base Sequence; D015246:Deoxyribonuclease EcoRI; D005091:Exons; D006801:Humans; D008969:Molecular Sequence Data; D015260:Neprilysin / Q000235:genetics*; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D015719:Single-Strand Specific DNA and RNA Endonucleases; D016385:TATA Box; D014158:Transcription, Genetic
D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D015246:Deoxyribonuclease EcoRI; D015719:Single-Strand Specific DNA and RNA Endonucleases; D015260:Neprilysin