Apoptosis and functional Fas antigen in rheumatoid arthritis synoviocytes.
OBJECTIVE
To determine whether apoptosis occurs in rheumatoid arthritis (RA) synoviocytes, and if this phenomenon is dependent on the Fas/Apo-1 pathway.


METHODS
Apoptotic change in vivo was examined in RA synovial cells by several standard methods. The ability of cells to undergo Fas-induced apoptosis was determined in vitro.


RESULTS
Typical apoptotic change was demonstrated in RA synovial cells by each method. Anti-Fas antibody induced apoptotic synovial cell death in vitro.


CONCLUSIONS
This is the first reported study to demonstrate apoptosis in RA synovial cells. The findings indicate that rheumatoid synoviocytes undergo Fas-mediated apoptosis.
Nakajima
T
T
Division of Rheumatology and Molecular Imunology, St. Marianna University, Kawasaki, Japan.
Aono
H
H
Hasunuma
T
T
Yamamoto
K
K
Shirai
T
T
Hirohata
K
K
Nishioka
K
K
D000906:Antibodies / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis* / Q000276:immunology; D017209:Apoptosis; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000473:pathology*; D005821:Genetic Techniques; D006801:Humans; D008854:Microscopy, Electron; D013583:Synovial Membrane / Q000276:immunology* / Q000473:pathology*; D019014:fas Receptor
D000906:Antibodies; D000954:Antigens, Surface; D019014:fas Receptor