Failure of genotoxic carcinogens to produce tumors in human skin xenografts transplanted to SCID mice.
Chemical carcinogenesis of human skin was investigated using human skin xenografts (16 full thickness and 48 split thickness skin grafts) transplanted to CB-17-scid (SCID) mice. Topical application of a carcinogen, i.e. 7,12-dimethylbenz[a]anthracene (DMBA), benzo[a]pyrene, methylcholanthrene or N-methyl-N'-nitro-N-nitrosoguanidine, to the human skin xenografts once a week for 25-30 weeks failed to produce skin tumors. Both DMBA application plus UV-B irradiation and alternate applications of the above four carcinogens in combination with UV-B irradiation also failed to produce tumors. All of these treatments induced skin papillomas in skins of host SCID mice. DMBA induced skin papillomas in allogenic CD-1 mouse skin grafts transplanted to SCID mice. These results indicate that susceptibility of human skin to these carcinogenic stimuli is much lower than that of mouse skin.
Urano
K
K
Central Institute for Experimental Animals, Kawasaki, Japan.
Katakai
Y
Y
Tokuda
Y
Y
Ueyama
Y
Y
Nomura
T
T
Yamamoto
S
S
D000818:Animals; D001711:Biotransformation; D002273:Carcinogens / Q000493:pharmacokinetics / Q000633:toxicity*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D010212:Papilloma / Q000139:chemically induced; D011084:Polycyclic Aromatic Hydrocarbons / Q000493:pharmacokinetics / Q000633:toxicity; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012878:Skin Neoplasms / Q000139:chemically induced*; D016038:Skin Transplantation; D014183:Transplantation, Heterologous
D002273:Carcinogens; D011084:Polycyclic Aromatic Hydrocarbons