title: [Oocyte maturity and quality: value of intracytoplasmic sperm injection. Fertility of microinjected oocytes after in vitro maturation].
abstract: Many studies in IVF practice, have tried to assess the maturity and quality of oocytes prior to insemination and relate it to IVF efficiency and to the pattern of ovarian stimulation. They were based on the indirect evaluation of the aspect of the cumulus-corona-cell complex (CCC) which was rapidly shown to be poorly correlated to the oocyte nuclear and cytoplasmic maturity in stimulated cycles. For sperm microinjection procedures, oocytes need to be peeled off from any follicular cell through hyaluronidase action in order to gain an easy access to the zona pellucida and the ooplasm. If therefore becomes possible to precisely known at recovery the nuclear status of the oocyte cohort as well as the rate of degenerative gametes. Immature oocytes can be further matured in vitro. Moreover, the ICSI procedure (intracytoplasmic sperm injection) allows a direct assessment of the cytoplasmic maturation, whatever the maturity of the ZP and its receptors and of the plasma membrane. On a preliminary evaluation of 70 ICSI cycles performed in our collaborative group from september to november 1994 and leading to a 24 % pregnancy rate per oocyte pick-up we focused on the true maturation of the oocyte cohort, its outcome and correlation with ICSI efficiency and stimulation protocol. On the 760 oocytes, 13.9% were atretic, 9% at the germinal vesicle stage (GV), 3.4% in metaphase 1 and 73.7% in metaphase 2 at recovery.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Hpital Necker, Paris.
authors.forename: A M
authors.initials: AM
authors.lastname: Junca
authors.forename: J
authors.initials: J
authors.lastname: Mandelbaum
authors.forename: J
authors.initials: J
authors.lastname: Belaïsch-Allart
authors.forename: J
authors.initials: J
authors.lastname: Salat-Baroux
authors.forename: M
authors.initials: M
authors.lastname: Plachot
authors.forename: J M
authors.initials: JM
authors.lastname: Antoine
authors.forename: J M
authors.initials: JM
authors.lastname: Mayenga
authors.forename: D
authors.initials: D
authors.lastname: Delafontaine
authors.forename: J
authors.initials: J
authors.lastname: Cohen
mesh_terms: D000328:Adult; D015331:Cohort Studies; D003593:Cytoplasm; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008677:Metaphase; D008845:Microinjections / Q000379:methods; D009865:Oocytes / Q000254:growth & development*; D011247:Pregnancy; D011256:Pregnancy Outcome