Identification of beta-1,6-glucosylated cell wall proteins in yeast and hyphal forms of Candida albicans.
Several cell wall proteins released from yeast and hyphal cells of Candida albicans by laminarinase reacted with an affinity-purified antiserum raised against beta-1,6-glucan. Binding of the antiserum was competitively inhibited by beta-1,6-glucan, but not by beta-1,3-glucan or isolated N-chains. Immunodetection was completely abolished when the proteins were treated with periodate. These results demonstrate that the laminarinase-released wall proteins of C. albicans possess an epitope consisting of beta-1,6-linked glucose residues. The yeast form of C. albicans contained four beta-1,6-glucosylated wall proteins, an Endo H-resistant protein of 125 kDa and three glycoproteins which became only detectable after Endo H digestion and had a molecular mass of 320, 170 and 44 kDa, respectively. As for the hyphal form, a different set of beta-1,6-glucosylated wall proteins was found consisting of two Endo H-resistant glycoproteins of 125 and 80 kDa, respectively, and two glycoproteins that after Endo H digestion had a molecular mass of 320 and 38 kDa, respectively. Sodium dodecyl sulfate-extractable wall proteins and medium proteins did not react with the beta-1,6-glucan antiserum. The beta-1,6-glucan epitope could be removed by aqueous hydrofluoric acid indicating that the epitope is phosphodiester-linked to the cell wall proteins. It is speculated that the epitope forms part of a GPI-anchor and might be involved in the anchoring of mannoproteins into the cell wall.
Kapteyn
J C
JC
Institute of Molecular Cell Biology, University of Amsterdam, The Netherlands.
Montijn
R C
RC
Dijkgraaf
G J
GJ
Klis
F M
FM
D002176:Candida albicans / Q000737:chemistry* / Q000648:ultrastructure; D002473:Cell Wall / Q000737:chemistry; D000939:Epitopes / Q000032:analysis; D005656:Fungal Proteins / Q000032:analysis*; D005936:Glucans / Q000378:metabolism*; D006031:Glycosylation; D008562:Membrane Glycoproteins / Q000032:analysis*; D047071:beta-Glucans
D000939:Epitopes; D005656:Fungal Proteins; D005936:Glucans; D008562:Membrane Glycoproteins; D047071:beta-Glucans; C025363:mannoproteins; C064197:beta-1,6-glucan