title: Crystallization and preliminary X-ray crystallographic studies on Type III antifreeze protein.
abstract: Type III antifreeze protein, more specifically the recombinant QAE-Sephadex-binding isoform, has been crystallized in 50-55% saturated ammonium sulfate, 0.1 M sodium acetate, pH 4.0-4.5. The resultant crystals belong to the orthorhombic space group P212121 with a = 32.60 A, b = 39.00 A, and c = 46.57 A and diffract to at least 1.7 A. A set of 1.7-A native data has been collected, with completeness 93.4% and Rsym of 0.069. Initial screening for heavy-atom derivatives has yielded a Pt-bound derivative.
authors.affiliation: Laboratory of Molecular Biophysics, University of Oxford, United Kingdom.
authors.forename: Z
authors.initials: Z
authors.lastname: Jia
authors.forename: C I
authors.initials: CI
authors.lastname: DeLuca
authors.forename: P L
authors.initials: PL
authors.lastname: Davies
mesh_terms: D000818:Animals; D021343:Antifreeze Proteins, Type III; D003460:Crystallization; D018360:Crystallography, X-Ray; D004524:Eels; D005615:Freezing; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D021343:Antifreeze Proteins, Type III; D011506:Proteins; C092813:RD3 protein, eel; D011994:Recombinant Proteins