title: Molecular subtyping scheme for serotypes HS1 and HS4 of Campylobacter jejuni.
abstract: We describe a molecular subtyping scheme for two principal O (heat-stable [HS]) serotypes of Campylobacter jejuni, HS1 and the HS4 complex. A 16S rRNA gene-specific probe confirmed that almost all the C. jejuni strains had three copies of this gene, and strains could be assigned with complete typeability to 1 of 16 combined (Pst1 and HaeIII) 16S ribotypes. Macrorestriction profiles (mrps) consisting of up to 10 SmaI fragments from approximately 40 to approximately 480 kbp were resolved by pulsed-field gel electrophoresis (PFGE). There were 11 mrps among the HS1 strains and 9 mrps among HS4 strains which corresponded to valid types--they occurred in multiple isolates, hosts, places, and times. There were 14 additional single-strain mrp fingerprints in HS1 and 20 in HS4. PFGE exhibited complete typeability when formaldehyde fixation of cells was employed, and PFGE was generally more differential than ribotyping. The data presented elucidate a high-resolution genotypic subtyping scheme for these common subspecific phenotypes of C. jejuni, which is both coherent and efficient for epidemiological purposes.
authors.affiliation: National Collection of Type Cultures, Central Public Health Laboratory, London, United Kingdom.
authors.forename: R J
authors.initials: RJ
authors.lastname: Owen
authors.forename: K
authors.initials: K
authors.lastname: Sutherland
authors.forename: C
authors.initials: C
authors.lastname: Fitzgerald
authors.forename: J
authors.initials: J
authors.lastname: Gibson
authors.forename: P
authors.initials: P
authors.lastname: Borman
authors.forename: J
authors.initials: J
authors.lastname: Stanley
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D016123:Campylobacter jejuni / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D002417:Cattle; D004269:DNA, Bacterial / Q000235:genetics; D004285:Dogs; D005784:Gene Amplification; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012703:Serotyping; D006113:United Kingdom
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S