title: Novel assay for the influenza virus M2 channel activity.
abstract: The influenza A M2 ion channel was expressed and activity characterized in Xenopus oocytes. Based on the activation properties of the channels, a high throughput, non-electrophysiological screening assay was developed in order to identify novel inhibitors of the channel. This will facilitate discovery of novel agents to treat influenza viral infections.
authors.affiliation: Department of Protein Biochemistry, Searle Research and Development, St. Louis, MO.
authors.forename: K
authors.initials: K
authors.lastname: Giffin
authors.forename: R K
authors.initials: RK
authors.lastname: Rader
authors.forename: M H
authors.initials: MH
authors.lastname: Marino
authors.forename: R W
authors.initials: RW
authors.lastname: Forgey
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000032:analysis*; D008564:Membrane Potentials; D009865:Oocytes; D011994:Recombinant Proteins / Q000032:analysis; D014763:Viral Matrix Proteins / Q000032:analysis* / Q000235:genetics; D014982:Xenopus laevis; D000086082:Viroporin Proteins
chemical_list: D007473:Ion Channels; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins; C031540:M-protein, influenza virus; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins