title: Antigenic differences between a field isolate and vaccine strains of bovine viral diarrhea virus.
abstract: Antigenic diversity among the bovine viral diarrhea virus (BVDV) cytopathic strains 87-2552 (field isolate) and NADL and Singer (prototype strains) was demonstrated with monoclonal antibodies (MAbs) in enzyme-linked immunosorbent, immunofluorescence, virus neutralization, and immunoprecipitation assays. Two MAbs against BVDV strain 87-2552, designated D11 and B7, strongly neutralized this field strain and were specific for the 48-kDa glycoprotein of the virus. These two MAbs have different subisotypes, immunoglobulin G1 for D11 and immunoglobulin G3 for B7. MAbs against BVDV strains 87-2552 and NADL were specific for their respective strains in virus neutralization assays. The results indicated significant antigenic differences between BVDV strain 87-2552 and the NADL and Singer strains.
authors.affiliation: Department of Diagnostic Medicine/Pathology, Kansas State University, Manhattan 66506, USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Reddy
authors.forename: W
authors.initials: W
authors.lastname: Xue
authors.forename: S
authors.initials: S
authors.lastname: Rivera
authors.forename: H C
authors.initials: HC
authors.lastname: Minocha
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000940:Antigenic Variation; D000956:Antigens, Viral; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000821:virology; D002417:Cattle; D003588:Cytopathogenic Effect, Viral / Q000276:immunology; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D051379:Mice; D009500:Neutralization Tests; D011233:Precipitin Tests; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D014765:Viral Vaccines