The segmental precision of the motor projection to the intercostal muscles in the developing chicken embryo. A differential labelling study using fluorescent tracers.
Each skeletal muscle in the vertebrate is innervated by a group of motoneurons called a motoneuron pool. Retrograde labelling of single motoneuron pools has suggested that the arrangement of motoneuron pools innervating different limb muscles does not change during the embryonic period when more than 50% of the motoneurons die. In this study we retrogradely labelled neighbouring intercostal motoneuron pools differentially with latex microspheres or dextran amines coupled to fluorescent dyes. We then mapped the positions of the differentially labelled motoneurons in whole-mount preparations using a computer-aided drawing system. While the intercostal motoneuron pools are clearly segregated even at early stages, there is some intermingling at the rostral and caudal ends. We used a logistic regression to determine the extent of segmental overlap, and to facilitate a quantitative comparison of the overlap at different stages. Statistical analysis shows that the overlap (expressed as the percentage of the length of the overlapping motoneuron pools) decreases modestly during the period of motoneuron death. Computer simulations suggest that this decrease does not result from random motoneuron death alone; one alternative possibility is selective death of motoneurons in the overlap zone. Occasional "rogue" motoneurons, that is, motoneurons of one pool that scatter into the neighbouring pool, are still present at the end of the period of cell death, representing a potential source of "noise" in the establishment of segmental patterns of connectivity.
Stirling
R V
RV
Department of Biology, Open University, Milton Keynes.
Liestøl
K
K
Summerbell
D
D
Glover
J C
JC
D000818:Animals; D002642:Chick Embryo / Q000033:anatomy & histology / Q000254:growth & development* / Q000294:innervation; D003198:Computer Simulation; D003911:Dextrans; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007366:Intercostal Muscles / Q000196:embryology / Q000294:innervation*; D007840:Latex; D008863:Microspheres; D009046:Motor Neurons / Q000648:ultrastructure*; D012235:Rhodamines
D003911:Dextrans; D005452:Fluoresceins; D005456:Fluorescent Dyes; D007840:Latex; D012235:Rhodamines; C052526:fluorescein-dextran; C051311:rhodamine dextran