Expression of cytokeratin 8 and low molecular weight cytokeratins in human basal cell carcinoma.
The expression of low molecular weight cytokeratins (Cks) 7,8,18, 18 and high molecular weight cytokeratin 10 in 23 basal cell carcinomas (BCCs) was investigated using a panel of 14 different commercially available monoclonal antibodies (MoAbs) with specific anti-cytokeratin activity. Four of these MoAbs were directed against Ck 8. The results showed that Ck 8 was detected in all 23 BCCs using MoAb 4.1.18. Two of the MoAbs showed inconsistent staining for Ck 8 and one of them did not show any staining at all. Cytokeratins 7 and 19 were detected incosistently. Cytokeratins 18 and 10 were not detected in any of the 23 BCCs that were examined. The incosistent observations on the expression of Ck 8 in BCCs in this study could have been due to different epitopes of the different cytokeratins that were detected by the different MoAbs. The results of this study lead to recommendation that whenever possible a panel of different MoAbs directed against the same Ck(s) should be used in order to minimize the risk of obtaining incorrect experimental results.
Kooy
A J
AJ
Department of Dermato-Venereology, Erasmus University, Rotterdam, The Netherlands.
Tank
B
B
Vuzevski
V D
VD
Van Joost
T
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000276:immunology; D002280:Basal Cell Carcinoma / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007633:Keratins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D012878:Skin Neoplasms / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D009363:Neoplasm Proteins; D007633:Keratins