title: Birth dates and survival after axotomy of neurochemically defined subsets of trigeminal ganglion cells.
abstract: Trigeminal (V) ganglion cells with different neurochemical phenotypes or different birth dates are affected differently by neonatal axonal transection. The aim of the present study was to determine if V ganglion cell birth date and neurochemical phenotype were correlated and if these two variables could be related to responses to neonatal axonal transection. Immunocytochemistry, histochemistry, and [3H]thymidine labelling were used to determine the birth dates of V ganglion cells recognized by antibodies directed against neurofilament protein (NF), calcitonin gene-related peptide (CGRP), and substance P (SP) and those that bound the lectin Bandierea simplicifolia-I (BS-I). All V ganglion cells were born between embryonic days (E-) 9.5 and 14.5. All ganglion cells were born between E-9.5 and E-14.5. In a normalized population (percentages normalized to equal 100%), over 90% of NF-positive V ganglion cells were born between E-10.5 and E-12.5. The majority of CGRP-positive and SP-positive ganglion cells (> 90%) were generated from E-13.5 to E-14.5 and E-12.5 through E-14.5, respectively. Almost 85% of BS-I-positive ganglion cells were generated on E-12.5 through E-14.5. Previous results and additional data from this study indicated that NF- and BS-I-positive ganglion cells are proportionally more likely to be lost after neonatal axotomy and that SP-positive cells are more likely to remain. The percentage of CGRP-positive cells in the V ganglion was not significantly altered by neonatal infraorbital nerve transection. Overall, these findings do not indicate a strong relationship between cell birth date and the probability of survival after neonatal axonal damage for all V ganglion cell phenotypes.
authors.affiliation: Department of Anatomy, Medical College of Ohio, Toledo 43699, USA.
authors.forename: F A
authors.initials: FA
authors.lastname: White
authors.forename: N L
authors.initials: NL
authors.lastname: Chiaia
authors.forename: G J
authors.initials: GJ
authors.lastname: Macdonald
authors.forename: R W
authors.initials: RW
authors.lastname: Rhoades
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D001345:Autoradiography; D001369:Axons / Q000502:physiology*; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D007150:Immunohistochemistry; D037102:Lectins; D016900:Neurofilament Proteins / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology; D010641:Phenotype; D037121:Plant Lectins; D051381:Rats; D013373:Substance P / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D012668:Trigeminal Ganglion / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism
chemical_list: C045475:Griffonia simplicifolia lectins; D037102:Lectins; D016900:Neurofilament Proteins; D037121:Plant Lectins; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide; D013936:Thymidine