The jellyfish green fluorescent protein: a new tool for studying ion channel expression and function.
Two methods are described for using the jellyfish green fluorescent protein (GFP) as a reporter gene for ion channel expression. GFP fluorescence can be used to identify the transfected cells, and to estimate the relative levels of ion channel expression, in cotransfection experiments. A GFP-NMDAR1 chimera can be constructed that produces a functional, fluorescent receptor subunit. These methods should facilitate studies of ion channel expression, localization, and processing.
Marshall
J
J
Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520.
Molloy
R
R
Moss
G W
GW
Howe
J R
JR
Hughes
T E
TE
D000818:Animals; D002460:Cell Line; D005071:Evoked Potentials; D049452:Green Fluorescent Proteins; D006801:Humans; D015640:Ion Channel Gating; D007473:Ion Channels / Q000096:biosynthesis* / Q000502:physiology; D007668:Kidney; D008164:Luminescent Proteins / Q000032:analysis / Q000096:biosynthesis*; D046911:Macromolecular Substances; D008856:Microscopy, Fluorescence / Q000379:methods; D018408:Patch-Clamp Techniques; D016133:Polymerase Chain Reaction; D016194:Receptors, N-Methyl-D-Aspartate / Q000096:biosynthesis* / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D037401:Scyphozoa; D014162:Transfection / Q000379:methods
D007473:Ion Channels; D008164:Luminescent Proteins; D046911:Macromolecular Substances; D016194:Receptors, N-Methyl-D-Aspartate; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins