Effect of oat saponins and different types of dietary fibre on the digestion of carbohydrates.
The effects of oat saponins (a mixture of avenacosides A and B) and dietary fibre (cellulose and guar gum) on the disaccharidase activities in the proximal small intestine of the rat were investigated. The influence of avenacosides A and B on the activity of disaccharidases and alpha-amylase (EC 3.2.1.1) was also studied in vitro. In vivo, oat diets with three avenacoside contents (negligible, normal and twice normal) were used. No significant differences in sucrase (EC 3.2.1.48), maltase (EC 3.2.1.20), trehalase (EC 3.2.1.28) and lactase (EC 3.2.1.21) activities were found between the oat groups after 19 d feeding. The rats that were given cellulose tended to have higher disaccharidase activities compared with the other groups. The avenacosides inhibited the lactase activity significantly in vitro while no or small effects on the other disaccharidases were found. In contrast, the in vitro hydrolysis of starch by alpha-amylase was increased in the presence of saponins, probably due to their detergent effect. Thus, the in vitro studies showed that the avenacosides could influence the enzyme activities. In vivo, these effects are probably minor due to the low avenacoside concentrations found in oats.
Onning
G
G
Department of Applied Nutrition and Food Chemistry, Lund University, Sweden.
Asp
N G
NG
D000818:Animals; D018554:Avena; D004040:Dietary Carbohydrates / Q000378:metabolism*; D004043:Dietary Fiber / Q000494:pharmacology*; D004063:Digestion / Q000187:drug effects*; D004186:Disaccharidases / Q000187:drug effects / Q000378:metabolism; D007413:Intestinal Mucosa / Q000201:enzymology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000494:pharmacology*; D000516:alpha-Amylases / Q000187:drug effects / Q000378:metabolism
D004040:Dietary Carbohydrates; D004043:Dietary Fiber; D012503:Saponins; D004186:Disaccharidases; D000516:alpha-Amylases