Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase.
Crystallization of membrane proteins, a prerequisite for their X-ray crystallographic analysis, remains difficult. Here, we demonstrate that the crystallization of the cytochrome c oxidase from Paracoccus denitrificans can be mediated by co-crystallization with an antibody Fv fragment. The crystals obtained contain all four subunits of this membrane protein complex and the Fv fragment. The approach of co-crystallizing membrane proteins with antibody fragments should be useful in obtaining well-ordered crystals of membrane proteins in general.
Ostermeier
C
C
Max-Planck-Institut für Biophysik, Heinrich-Hoffmann, Frankfurt/Main, Germany.
Iwata
S
S
Ludwig
B
B
Michel
H
H
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins / Q000737:chemistry; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods*; D003576:Electron Transport Complex IV / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D010231:Paracoccus denitrificans / Q000201:enzymology; D014961:X-Ray Diffraction
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D007128:Immunoglobulin Fragments; D008565:Membrane Proteins; C055966:immunoglobulin Fv; D003576:Electron Transport Complex IV