title: Complete removal of mycoplasma from viral preparations using solvent extraction.
abstract: Mycoplasma contamination of two alphavirus isolates and adenovirus 5 was completely removed using a very simple, rapid protocol based on solvent extraction. Treated virus preparations remained free of mycoplasma after 6 months in culture.
authors.affiliation: Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.
authors.forename: M
authors.initials: M
authors.lastname: La Linn
authors.forename: A J
authors.initials: AJ
authors.lastname: Bellett
authors.forename: P G
authors.initials: PG
authors.lastname: Parsons
authors.forename: A
authors.initials: A
authors.lastname: Suhrbier
mesh_terms: D000260:Adenoviruses, Human / Q000302:isolation & purification / Q000502:physiology*; D001431:Bacteriological Techniques; D006690:Bisbenzimidazole; D006367:HeLa Cells; D006801:Humans; D009174:Mycoplasma / Q000302:isolation & purification*; D012398:Ross River virus / Q000302:isolation & purification / Q000502:physiology*; D012997:Solvents; D014773:Virology / Q000379:methods
chemical_list: D012997:Solvents; D006690:Bisbenzimidazole