Comparison of the enzymatic activities of human galactokinase GALK1 and a related human galactokinase protein GK2.
The GALK1 cDNA encoding human galactokinase was recently cloned and its cognate GALK1 gene shown to be involved in galactokinase deficient galactosemia. Previously, a separate human galactokinase cDNA, GK2, was cloned by complementation of a galactokinase deficient yeast mutant; however, the galactokinase activity of GK2 was not demonstrated in mammalian cells. To compare the relative galactokinase activity of GALK1 and GK2, their corresponding cDNAs were expressed in COS cells. Northern blot analysis indicated that both cDNAs were transcribed into mRNA transcripts of the expected size; however, only the GALK1 cDNA produced high levels of galactokinase activity. This result would suggest that GALK1 is the major enzyme for galactose metabolism while the role of GK2 remains uncertain.
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Department of Ophthalmology, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA.
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Bergsma
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Stambolian
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D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005686:Galactokinase / Q000235:genetics / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D014162:Transfection
D018076:DNA, Complementary; D012333:RNA, Messenger; D005686:Galactokinase; D012194:RNA-Directed DNA Polymerase; D005690:Galactose