title: Association and coexpression of fatty-acid-binding protein and glycoprotein CD36 in the bovine mammary gland.
abstract: The involvement of glycoprotein CD36 and fatty-acid-binding protein (FABP) in cellular growth, differentiation, lipid transport and metabolism led us to examine the possible biochemical and physiological relationship(s) between these two proteins. We investigated three aspects of this relationship. We first attempted to identify any physical complex formed between CD36 and FABP in bovine milk fat globule membranes. These membranes are the product of mammary gland secretory epithelial cells. The second aspect studied was the effect of synthetic peptide analogs to the C-terminus (amino acid residues 121-131) of bovine mammary gland FABP on cell proliferation, as a result of the interaction of these peptides with the ectodomain of CD36. Finally, mammary gland CD36 and FABP coexpression was defined at different stages of lactation and during involution. Immunoprecipitation, Western immunoblotting with anti-FABP and anti-CD36, Northern-blot analysis and a mammary epithelial cell proliferation assay demonstrated that: (a) bovine milk fat globule membranes contain the complex of CD36 and FABP, and that this complex is, most likely, formed as a result of FABP binding to the cytoplasmic segments of CD36; (b) synthetic analog of the C-terminus of FABP with the sequence Val-Thr-Cys, identical to the sequence found in the CD36-binding domain of thrombospondin, was a more potent inhibitor of bovine mammary gland epithelial cell proliferation than a synthetic peptide with the Val-Cys-Thr sequence; (c) the expression of FABP and CD36 is related to the state of mammary cell differentiation, since it reaches its maximum during lactation and declines during the involutionary period.
authors.affiliation: Department of Animal Science, Cornell University, Ithaca, NY 14853, USA.
authors.forename: V L
authors.initials: VL
authors.lastname: Spitsberg
authors.forename: E
authors.initials: E
authors.lastname: Matitashvili
authors.forename: R C
authors.initials: RC
authors.lastname: Gorewit
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology; D015153:Blotting, Western; D018955:CD36 Antigens; D002352:Carrier Proteins / Q000032:analysis* / Q000276:immunology / Q000494:pharmacology; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D050556:Fatty Acid-Binding Proteins; D005260:Female; D008321:Mammary Glands, Animal / Q000737:chemistry* / Q000166:cytology / Q000187:drug effects; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D010446:Peptide Fragments / Q000494:pharmacology; D011233:Precipitin Tests
chemical_list: D015703:Antigens, CD; D018955:CD36 Antigens; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D009363:Neoplasm Proteins; D010446:Peptide Fragments