Deregulated c-Fos/AP-1 modulates expression of the cyclin and the cdk gene in splenic B cells stimulated with lipopolysaccharide.
Overexpression of c-Fos/AP-1 augments proliferation of splenic B cells stimulated with lipopolysaccharide (LPS). To elucidate mechanisms of the augmentation by c-Fos/AP-1, a cell cycle of the LPS-activated B cells from c-fos transgenic mice was analyzed. Cell cycle progression into the S phase was accelerated in the c-fos B cells. Expression of genes related to the cell cycle progression was examined in these B cells. Amount of cyclin D3 and cdk4 mRNA increased in the c-fos B cells at 6 h earlier than that in the control B cells, indicating that the kinetics of these mRNA expressions correlate with the acceleration of cell cycle progression. Furthermore, cyclin D1 and cyclin E mRNA were detected in the c-fos B cells but not in the control B cells. These results indicate that deregulated c-Fos/AP-1 modulates expression of the cyclin and the cdk gene in splenic B cells stimulated with LPS. These modulations may accelerate cell cycle progression and augment proliferation of the B cells.
Phuchareon
J
J
Division of Developmental Genetics, Chiba University School of Medicine, Japan.
Tokuhisa
T
T
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D001483:Base Sequence; D015152:Blotting, Northern; D018844:Cyclin-Dependent Kinases / Q000235:genetics*; D016213:Cyclins / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000502:physiology*; D012313:RNA / Q000032:analysis / Q000235:genetics; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013268:Stimulation, Chemical; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000502:physiology*
D016213:Cyclins; D008070:Lipopolysaccharides; D016760:Proto-Oncogene Proteins c-fos; D018808:Transcription Factor AP-1; D012313:RNA; D018844:Cyclin-Dependent Kinases