Effects of arginine butyrate and tributyrylxylitol on cultured human sarcoma cells.
The effects of arginine butyrate and tributyrylxylitol were studied comparatively in a human sarcoma cell line. Both induced important structural and functional modifications suggestive of cell differentiation, as shown by the dose-dependent increase of alkaline phosphatase activity and total protein content, at concentrations ranging from 1mM to 5 mM expressed in butyrate equivalents. hCG beta-subunit present in the culture medium increased with differentiation. Our results show that most of the differentiation changes previously reported for sodium butyrate in cancer cell lines are also produced by both drugs. Tributyrylxylitol appears to be the more potent and effective inducer of differentiation but its use is limited << in vitro >> on account of its relative toxicity at concentrations above 3 mM butyrate equivalents.
Vincent-Fiquet
O
O
Laboratoire d'Hormonologie, CHR, Amiens, France.
Rogez
J C
JC
Boitte
F
F
Brazier
M
M
Desmet
G
G
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001120:Arginine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000378:metabolism; D018997:Chorionic Gonadotropin, beta Subunit, Human; D003470:Culture Media; D006801:Humans; D046911:Macromolecular Substances; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D012509:Sarcoma / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014993:Xylitol / Q000031:analogs & derivatives* / Q000494:pharmacology
D000970:Antineoplastic Agents; D002087:Butyrates; D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D003470:Culture Media; D046911:Macromolecular Substances; D009363:Neoplasm Proteins; D010446:Peptide Fragments; C091856:tributyrylxylitol; D001120:Arginine; D000469:Alkaline Phosphatase; C033018:arginine butyrate; D014993:Xylitol