Reduced antigen-presenting function of human Epstein-Barr virus (EBV)-B cells and monocytes after UVB radiation is accompanied by decreased expression of B7, intercellular adhesion molecule-1 (ICAM-1) and LFA-3.
In this study, the effect of ultraviolet-B (UVB) radiation on antigen-presenting function was studied, to investigate whether antigen-presenting cells (APC) are inhibited by UVB through a common mechanism. Two types of human APC were used: EBV-B cells and monocytes, and these were irradiated in vitro with single low doses of UVB (range 0-200 J/m2). Irradiation of EBV-B cells or monocytes resulted in similar dose-dependent reduction in APC function, when determined by the allogeneic mixed leucocyte reaction (MLR) or Candida albicans- or tetanus toxoid-specific T cell response. Our study shows that the reduced APC function was not likely to be caused by alterations in antigen processing or cytokine production. However, UVB-irradiated APC displayed marked changes in adhesion molecule expression. Irradiated EBV-B cells showed reduced expression of ICAM-1 (30%), LFA-3 (25%) and B7-1 (35%), while expression of HLA-DR, CD19 and LFA-1 was not affected. UVB irradiation of monocytes did result in reduction in the expression of HLA-DR (30%), LFA-3 (40%), ICAM-1 (65%) AND B7-1 and B7-3 (90%), but had no effect on CD14, LFA-1 and ICAM-3 expression. Addition of non-irradiated cells (but not the supernatant of these cells) or CD28 antibodies partly restored T cell activation, indicating that UVB-induced reduction in APC function is at least partly mediated via impairment of co-stimulatory molecule expression.
Kremer
I B
IB
Department of Dermatology, University of Amsterdam, The Netherlands.
Bos
J D
JD
Teunissen
B M
BM
D017951:Antigen Presentation / Q000276:immunology / Q000528:radiation effects*; D015703:Antigens, CD / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology / Q000528:radiation effects* / Q000821:virology; D018122:B7-1 Antigen / Q000096:biosynthesis*; D018968:CD58 Antigens; D002461:Cell Line, Transformed; D002470:Cell Survival; D004854:Herpesvirus 4, Human; D006801:Humans; D016130:Immunophenotyping; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D009000:Monocytes / Q000276:immunology / Q000528:radiation effects*; D014466:Ultraviolet Rays
D015703:Antigens, CD; D018122:B7-1 Antigen; D018968:CD58 Antigens; D008562:Membrane Glycoproteins; D018799:Intercellular Adhesion Molecule-1