Rapid detection of methicillin resistance in coagulase-negative staphylococci by commercially available fluorescence test.
A commercially available fluorescence test (Crystal MRSA ID System; BBL) for the rapid detection of methicillin resistance in Staphylococcus aureus was evaluated for the detection of methicillin resistance in coagulase-negative staphylococci. The assay was compared with the agar dilution method and mecA detection by PCR. Provided that one uses an inoculum equivalent to a no. 2 McFarland standard instead of a no. 0.5 McFarland standard, which is used for S. aureus, the Crystal MRSA ID System detects methicillin resistance among coagulase-negative staphylococci with sensitivity, specificity, and positive and negative predictive values of 98.5, 95.4, 96.3, and 98.1%, respectively.
Ieven
M
M
Laboratory for Microbiology, University Hospital Antwerp, Edegem, Belgium.
Jansens
H
H
Ursi
D
D
Verhoeven
J
J
Goossens
H
H
D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D003030:Coagulase / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005453:Fluorescence; D005798:Genes, Bacterial; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013210:Staphylococcus / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics
D003030:Coagulase