title: CD1-restricted T cell recognition of microbial lipoglycan antigens.
abstract: It has long been the paradigm that T cells recognize peptide antigens presented by major histocompatibility complex (MHC) molecules. However, nonpeptide antigens can be presented to T cells by human CD1b molecules, which are not encoded by the MHC. A major class of microbial antigens associated with pathogenicity are lipoglycans. It is shown here that human CD1b presents the defined mycobacterial lipoglycan lipoarabinomannan (LAM) to alpha beta T cell receptor-bearing lymphocytes. Presentation of these lipoglycan antigens required internalization and endosomal acidification. The T cell recognition required mannosides with alpha(1-->2) linkages and a phosphotidylinositol unit. T cells activated by LAM produced interferon gamma and were cytolytic. Thus, an important class of microbial molecules, the lipoglycans, is a part of the universe of foreign antigens recognized by human T cells.
authors.affiliation: Division of Dermatology, University of California at Los Angeles (UCLA) School of Medicine 90095, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Sieling
authors.forename: D
authors.initials: D
authors.lastname: Chatterjee
authors.forename: S A
authors.initials: SA
authors.lastname: Porcelli
authors.forename: T I
authors.initials: TI
authors.lastname: Prigozy
authors.forename: R J
authors.initials: RJ
authors.lastname: Mazzaccaro
authors.forename: T
authors.initials: T
authors.lastname: Soriano
authors.forename: B R
authors.initials: BR
authors.lastname: Bloom
authors.forename: M B
authors.initials: MB
authors.lastname: Brenner
authors.forename: M
authors.initials: M
authors.lastname: Kronenberg
authors.forename: P J
authors.initials: PJ
authors.lastname: Brennan
mesh_terms: D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D018949:Antigens, CD1; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002460:Cell Line; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007918:Leprosy / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D009166:Mycobacterium leprae / Q000276:immunology; D010716:Phosphatidylinositols / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013045:Species Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D008070:Lipopolysaccharides; D010716:Phosphatidylinositols; D016693:Receptors, Antigen, T-Cell, alpha-beta; C050016:lipoarabinomannan; C034154:phosphatidylinositol mannoside; D015847:Interleukin-4; D007371:Interferon-gamma