Identification of channel-forming activity in the cell wall of Corynebacterium glutamicum.
The cell wall of the gram-positive Corynebacterium glutamicum was prepared. It contained an ion-permeable channel with a single-channel conductance of about 6 nS in 1 M KCl. The mobility sequence of the ions in the channel is similar to that in the aqueous phase, suggesting that it is a water-filled channel wide enough to allow unhindered diffusion of ions. The results indicate that we have identified the hydrophilic pathway through the mycolic acid layer of C. glutamicum.
Niederweis
M
M
Lehrstuhl für Biotechnologie, Biozentrum der Universität Würzburg, Germany.
Maier
E
E
Lichtinger
T
T
Benz
R
R
Krämer
R
R
D002458:Cell Fractionation; D002473:Cell Wall; D003352:Corynebacterium / Q000502:physiology*; D004553:Electric Conductivity; D015640:Ion Channel Gating; D007473:Ion Channels / Q000502:physiology*
D007473:Ion Channels