A novel IL-4 responsive element of the E alpha MHC class II promoter that binds to an inducible factor.
Interleukin-4 (IL-4) is a lymphokine with important role in the growth and differentiation of T and B lymphocytes. In the latter, IL-4 induces transcriptionally MHC class II gene expression. Using the M12 mouse lymphoid cell line, we have determined an IL-4 response sequence (ILRS) in the proximal promoter region of the E alpha class II gene. The ILRS extends from -80 to -111 and includes the MHC class II X motif and 19 bp of additional 5' sequence. In mouse lymphoid cells, IL-4 activates a complex (Nuclear Factor-IL-4, NFIL-4), that binds to a novel element within the ILRS. Similar IL-4 inducible complexes bind to the interferon-gamma response element of the Fc gamma receptor (GRR), the acute phase response element (APRE) of the alpha 2 macroglobulin promoters and an INF beta promoter site, overlapping the PRDII/NF kappa B element. The factor contacts all these elements through their common GGAA motif. NFIL-4 is immunologically unrelated to NF kappa B or STAT 1 proteins that also recognize the above elements. Activation of NFIL-4 requires tyrosine phosphorylation, occurs within 2 min and persists as long as IL-4 is present. NFIL-4 has an apparent molecular weight of 75 kDa as determined by sedimentation through glycerol gradients.
Kretsovali
A
A
Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, Heraklion, Crete, Greece.
Papamatheakis
J
J
D000818:Animals; D001483:Base Sequence; D002457:Cell Extracts; D002467:Cell Nucleus; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D005802:Genes, MHC Class II / Q000235:genetics*; D016899:Interferon-beta / Q000235:genetics; D015847:Interleukin-4 / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016328:NF-kappa B / Q000378:metabolism; D010766:Phosphorylation; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017452:Receptors, IgG / Q000235:genetics; D050794:STAT1 Transcription Factor; D015534:Trans-Activators / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000235:genetics
D002457:Cell Extracts; D004268:DNA-Binding Proteins; D016328:NF-kappa B; D017452:Receptors, IgG; D050794:STAT1 Transcription Factor; C494080:Stat1 protein, mouse; D015534:Trans-Activators; D000511:alpha-Macroglobulins; D015847:Interleukin-4; D016899:Interferon-beta; D004247:DNA