Identification of MIP-1 alpha/LD78 as a monocyte chemoattractant released by the HTLV-I-transformed cell line MT4.
It is known that the HTLV-I-transformed cell line MT4 releases chemotactic activity for monocytes spontaneously. The MT4 monocyte chemoattractant was purified to homogeneity and sequencing of 25 amino acids revealed identity with the C-C chemokine macrophage inflammatory protein-1 alpha (MIP-1 alpha/LD78). An anti-MIP-1 alpha/LD78 rabbit antiserum substantially inhibited chemotaxis of the MT4 chemoattractant. MT4 cells constitutively expressed MIP-1 alpha/LD78 but not the C-C chemokines MCP-1, RANTES, and MIP-1 beta/Act2 and the C-X-C chemokines IL-8, gro alpha, and gro beta. MT4-derived MIP-1 alpha/LD78 was active on monocytes but was a weak chemoattractant for polymorphonuclear leukocytes. Thus, MIP-1 alpha/LD78 is a major monocyte chemoattractant released by HTLV-I-transformed T cells. Expression of MIP-1 alpha/LD78, a leukocyte chemotactic and myelosuppressive molecule, may play an important role in the manifestations of HTLV-I-related diseases.
Bertini
R
R
Laboratories of Biotechnology, Research Center Dompé S.p.A., L'Aquila, Italy.
Luini
W
W
Sozzani
S
S
Bottazzi
B
B
Ruggiero
P
P
Boraschi
D
D
Saggioro
D
D
Chieco-Bianchi
L
L
Proost
P
P
van Damme
J
J
D000595:Amino Acid Sequence; D002461:Cell Line, Transformed; D054407:Chemokine CCL4; D002630:Chemotactic Factors / Q000235:genetics* / Q000302:isolation & purification; D016207:Cytokines / Q000235:genetics*; D015490:HTLV-I Infections / Q000378:metabolism; D006801:Humans; D019402:Macrophage Inflammatory Proteins; D008969:Molecular Sequence Data; D018945:Monocyte Chemoattractant Proteins; D015846:Monokines / Q000235:genetics*; D012313:RNA / Q000032:analysis; D017421:Sequence Analysis
D054407:Chemokine CCL4; D002630:Chemotactic Factors; D016207:Cytokines; D019402:Macrophage Inflammatory Proteins; D018945:Monocyte Chemoattractant Proteins; D015846:Monokines; D012313:RNA