title: Invariance of textural features in image cytometry under variation of size and pixel magnitude.
abstract: Textural features of the granular structure of stained cell nuclei and nuclear sections derived from co-occurrence and run length matrices are often used for correlation with external (clinical) parameters. The representation of cell nuclei and nuclear sections vary considerably under changes of preparation and fixation conditions. Most obvious are changes in size e.g. by fixation as well as changes in the amount of bound stain. Computer-simulated variations in size and pixel magnitude of a set of images of cell nuclei stained with Feulgen were featured and compared. Resulting feature dependencies are used for the setting of parameters of feature extraction procedures as well as for the selection of invariant features for texture quantification of material with variations examined.
authors.affiliation: GSF Forschungszentrum für Umwelt und Gesundheit mbH, Institut für Pathologie, Oberschleissheim, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Rodenacker
mesh_terms: D002467:Cell Nucleus / Q000737:chemistry / Q000648:ultrastructure*; D004396:Coloring Agents; D007091:Image Processing, Computer-Assisted; D012394:Rosaniline Dyes; D013194:Staining and Labeling
chemical_list: D004396:Coloring Agents; C007722:Feulgen stain; D012394:Rosaniline Dyes