Mast cells, eosinophils and IgE-positive cells in the nasal mucosa of patients with vasomotor rhinitis. An immunohistochemical study.
Vasomotor rhinitis (VMR) is a disorder of unknown pathogenesis. Forty patients with VMR were carefully selected on the basis of inclusion and exclusion criteria proposed by Mygind and Weeke. Nasal biopsy specimens were taken in the patient group as well as in a group of ten controls. Brush cytology was also taken in the VMR group. Inflammatory cells were identified and counted in the nasal mucosa, with the use of immunohistochemical techniques and a panel of monoclonal antibodies. Eosinophils were studied with the use of BMK13, EG2, and Giemsa. Mast cells were studied with anti-chymase (B7), anti-tryptase (G3) and toluidine blue. Sections were stained with IgE as well. There was no significant difference in the number of eosinophils, mast cells and IgE-positive cells between the two groups. Additionally, in contrast with other reports, in sections that were double-stained with anti-chymase and anti-tryptase, single chymase-positive cells were found.
Blom
H M
HM
Department of Otorhinolaryngology, University Hospital Dijkzigt, Rotterdam, The Netherlands.
Godthelp
T
T
Fokkens
W J
WJ
Klein Jan
A
A
Holm
A F
AF
Vroom
T M
TM
Rijntjes
E
E
D000293:Adolescent; D000328:Adult; D000368:Aged; D000469:Alkaline Phosphatase / Q000032:analysis; D000921:Antibody-Producing Cells / Q000473:pathology; D001798:Blood Proteins / Q000032:analysis; D002452:Cell Count; D053818:Chymases; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000473:pathology*; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000032:analysis; D008407:Mast Cells / Q000473:pathology*; D008875:Middle Aged; D009297:Nasal Mucosa / Q000276:immunology / Q000473:pathology*; D012223:Rhinitis, Vasomotor / Q000276:immunology / Q000473:pathology*; D012260:Ribonucleases; D012697:Serine Endopeptidases / Q000032:analysis; D013194:Staining and Labeling; D014048:Tolonium Chloride; D053802:Tryptases
D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D018836:Inflammation Mediators; D014048:Tolonium Chloride; D007073:Immunoglobulin E; D012260:Ribonucleases; D000469:Alkaline Phosphatase; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases