title: Comparison of the binding of nicotinic agonists to receptors from human and rat cerebral cortex and from chick brain (alpha 4 beta 2) transfected into mouse fibroblasts with ion channel activity.
abstract: (-)-Nicotine, cytisine and carbachol evoked 86Rb efflux from mouse fibroblasts stably transfected with alpha 4 beta 2 chick brain nicotinic subunits. This response to (-)-nicotine was inhibited by mecamylamine and dihydro-beta-erythroidine and was mirrored by a rise in intracellular Ca2+ measured by microspectrofluorimetry. Lobeline and isoarecolone methiodide evoked no significant 86Rb from cells and unlike the above agonists displayed significantly different IC50 values for the displacement of [3H]nicotine from mammalian (rat and human cerebral cortex) and transfected fibroblast membranes.
authors.affiliation: MRC Neurochemical Pathology Unit, Newcastle General Hospital, Newcastle upon Tyne, UK.
authors.forename: J A
authors.initials: JA
authors.lastname: Court
authors.forename: E K
authors.initials: EK
authors.lastname: Perry
authors.forename: D
authors.initials: D
authors.lastname: Spurden
authors.forename: S
authors.initials: S
authors.lastname: Lloyd
authors.forename: J I
authors.initials: JI
authors.lastname: Gillespie
authors.forename: P
authors.initials: P
authors.lastname: Whiting
authors.forename: R
authors.initials: R
authors.lastname: Barlow
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D002642:Chick Embryo; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism; D016257:Fura-2; D006801:Humans; D007473:Ion Channels / Q000502:physiology; D051379:Mice; D018722:Nicotinic Agonists / Q000378:metabolism*; D051381:Rats; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000378:metabolism*; D012414:Rubidium Radioisotopes
chemical_list: D007473:Ion Channels; D018722:Nicotinic Agonists; D011978:Receptors, Nicotinic; D012414:Rubidium Radioisotopes; D002118:Calcium; D016257:Fura-2