In vitro assessment of compounds for anti-HIV activity.
Human immunodeficiency virus types 1 and 2 (HIV-1, -2), the etiological agents of AIDS, are retroviruses that replicate in CD4+ T lymphocytes, monocytes, and macrophages. Early anti-HIV therapies were directed at the step in the virus life cycle that was considered the most readily blocked: the transcription of viral RNA into its DNA copy by the viral enzyme reverse transcriptase (RT). Unfortunately, to date, patient therapies have been relatively unsuccessful and hampered by toxicological problems. This has promoted further research into inhibitors of HIV replication, which may act at alternative stages in the viral replicative cycle as well as more effective RT inhibitors. In order to facilitate such research, simple and accurate in vitro assays are highly desirable. Here we describe such assays that measure components of the HIV replicative cycle and are suitable for use within antiviral experiments.
Patience
C
C
Chester Beatty Labs, Institute of Cancer Research, London, UK.
Moore
J
J
Boyd
M
M
D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000494:pharmacology*; D001709:Biotechnology; D002460:Cell Line; D003124:Colorimetry / Q000379:methods; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D016655:HIV Core Protein p24 / Q000032:analysis / Q000235:genetics; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D015498:HIV-2 / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D008826:Microbial Sensitivity Tests / Q000379:methods*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D018894:Reverse Transcriptase Inhibitors; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D016655:HIV Core Protein p24; D010446:Peptide Fragments; D018894:Reverse Transcriptase Inhibitors; D012194:RNA-Directed DNA Polymerase