Changes in radiation sensitivity of human osteosarcoma cells after p53 introduction.
Human osteosarcoma SAOS-2 cells, which have a deletion in p53 gene, were transfected with plasmid pMSVneop53 containing human p53 cDNA and neomycin-resistance gene. Three clones (SAOS-MC10, SAOS-MC11 and SAOS-MC43) among 60 clones expressed p53 mRNA. No p53 protein was observed in SAOS-MC10, while SAOS-MC11 and SAOS-MC43 produced p53 protein. The molecular weight of p53 protein in SAOS-MC43 was lower than that in SAOS-MC11, SAOS-MC11 and SAOS-MC43 were more sensitive and more resistant, respectively, to ionizing radiation than the parental SAOS-2. We suggest that exogenous p53 protein might be one of the factors determining cellular radiosensitivity.
Miyakoshi
J
J
Department of Radiation Genetics, Faculty of Medicine, Kyoto University.
Yamagishi
N
N
Ohtsu
S
S
Takebe
H
H
D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000532:radiotherapy; D016158:Genes, p53 / Q000502:physiology*; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology* / Q000532:radiotherapy; D011836:Radiation Tolerance; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000528:radiation effects