title: Impact of erythrocytes on mouse embryonal development in vitro.
abstract: To elucidate the role of reactive oxygen species (ROS) in the development of mouse embryo, effects of superoxide dismutase (SOD), catalase and erythrocytes were studied in vitro. Oocytes were fertilized and 2-cell-cleaved embryos were cultured in the presence or absence of either erythrocytes, SOD or catalase. Under standard culture conditions, the fertilization and cleavage rates were 77.4 and 12.5%, respectively. In the presence of xanthine and xanthine oxidase, those rates decreased to 28.2 and 4.5%, respectively. The hazardous effect of ROS was completely inhibited by erythrocytes. These results suggested that small amounts of erythrocytes might effectively degrade ROS during the development of cultured embryos.
authors.affiliation: Department of Obstetrics and Gynecology, Osaka City University Medical School, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Matsuoka
authors.forename: Y
authors.initials: Y
authors.lastname: Fujino
authors.forename: S
authors.initials: S
authors.lastname: Ogita
authors.forename: M
authors.initials: M
authors.lastname: Inoue
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D046508:Culture Techniques; D005314:Embryonic and Fetal Development / Q000502:physiology*; D004912:Erythrocytes / Q000502:physiology*; D005260:Female; D006131:Growth Inhibitors / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000502:physiology; D019820:Xanthine; D014969:Xanthine Oxidase / Q000378:metabolism; D014970:Xanthines / Q000494:pharmacology
chemical_list: D006131:Growth Inhibitors; D017382:Reactive Oxygen Species; D014970:Xanthines; D019820:Xanthine; D002374:Catalase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase