title: Differential localization of lectin binding sites and neuropeptides in human dorsal root ganglia.
abstract: The subpopulations were compared of neurons in human dorsal root ganglia (DRG), as substance P, identified by somatostatin, Glycine max lectin (SBA) specific to terminal N-acetylgalactosamine, and Ulex europaeus I agglutinin (UEA-I) specific to L-fucose. The lectins and neuropeptides all bound to neurons of small diameter. Furthermore, the majority of the SBA binding neurons or somatostatin positive neurons were also UEA-I binding neurons. However, SBA binding neurons were not colocalized with somatostatin or substance P. Less than 20% of substance P positive neurons showed colocalization with L-fucosyl residues, and approximately 10% of L-fucosyl residues showed colocalization with substance P. Our results suggest that both L-fucose and terminal N-acetylgalactosamine containing neurons in the human DRG are subjected to different subpopulations from substance P or somatostatin positive neurons.
authors.affiliation: Department of Neurology, Kyoto University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nagao
authors.forename: N
authors.initials: N
authors.lastname: Oka
authors.forename: H
authors.initials: H
authors.lastname: Kamo
authors.forename: I
authors.initials: I
authors.lastname: Akiguchi
authors.forename: J
authors.initials: J
authors.lastname: Kimura
mesh_terms: D000116:Acetylgalactosamine / Q000032:analysis*; D000368:Aged; D000369:Aged, 80 and over; D005643:Fucose / Q000032:analysis*; D005727:Ganglia, Spinal / Q000737:chemistry*; D006801:Humans; D037102:Lectins; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000737:chemistry; D013004:Somatostatin / Q000032:analysis*; D013116:Spinal Cord / Q000737:chemistry*; D013373:Substance P / Q000032:analysis*
chemical_list: D037102:Lectins; D005643:Fucose; D013373:Substance P; D013004:Somatostatin; D000116:Acetylgalactosamine