Detection of the turkey pathogens Mycoplasma meleagridis and M. iowae by amplification of genes coding for rRNA.
PCR-based diagnostic tests using oligonucleotides specific to 16S rRNA were designed for the specific detection of the turkey pathogens Mycoplasma meleagridis and M. iowae. This method of detection was shown to be rapid, species specific, and unaffected by strain variation or the presence of other organisms. Detection of M. meleagridis in clinical samples by PCR was achieved and later confirmed by culture and growth inhibition. Definitive identification by culture and growth inhibition required up to 3 weeks, whereas positive results from PCR testing were obtained within a day and negative samples were confirmed within 4 days.
Boyle
J S
JS
Victorian Institute of Animal Science, Department of Agriculture, Attwood, Australia.
Good
R T
RT
Morrow
C J
CJ
D000818:Animals; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005784:Gene Amplification; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D009175:Mycoplasma Infections / Q000175:diagnosis / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods / Q000706:statistics & numerical data; D011201:Poultry Diseases / Q000175:diagnosis; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D014422:Turkeys / Q000382:microbiology*
D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S