title: Deleted in oral cancer-1 (doc-1), a novel oral tumor suppressor gene.
abstract: We have identified, isolated, and partially characterized doc-1, a novel cDNA sequence whose activity is consistent with a suppressor of hamster oral carcinogenesis. Doc-1 is an evolutionarily conserved gene exhibiting loss of heterozygosity and marked reduction in expression in malignant hamster oral keratinocytes. The full-length doc-1 cDNA encodes an 87 amino acid product that shows a significant homology to one of the seven novel genes induced in mouse fibroblasts by TNF-alpha. Transfection of the full-length doc-1 cDNA into malignant hamster oral keratinocytes alters the behavior of the recipients in terms of morphology, growth rate, and anchorage-independent growth, suggesting reversion of transformation phenotypes. We propose that doc-1 is a novel tumor suppressor gene in oral cancer development.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Boston 02114, USA.
authors.forename: R
authors.initials: R
authors.lastname: Todd
authors.forename: J
authors.initials: J
authors.lastname: McBride
authors.forename: T
authors.initials: T
authors.lastname: Tsuji
authors.forename: R B
authors.initials: RB
authors.lastname: Donoff
authors.forename: M
authors.initials: M
authors.lastname: Nagai
authors.forename: M Y
authors.initials: MY
authors.lastname: Chou
authors.forename: T
authors.initials: T
authors.lastname: Chiang
authors.forename: D T
authors.initials: DT
authors.lastname: Wong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor; D015603:Keratinocytes / Q000502:physiology; D008969:Molecular Sequence Data; D009062:Mouth Neoplasms / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
chemical_list: C096151:CDK2AP1 protein, human; D018076:DNA, Complementary; D011506:Proteins; D025521:Tumor Suppressor Proteins