title: Cloning and chromosomal localization of the human A2b adenosine receptor gene (ADORA2B) and its pseudogene.
abstract: To determine the chromosomal localization of the human A2b adenosine receptor, the corresponding genomic clone was isolated and used as a probe for fluorescence in situ hybridization to metaphase chromosomes. Partial sequence analysis of the A2b gene (AD-ORA2B) revealed an intron that interrupted the coding region corresponding to the second intracellular loop similar to that reported for A1 and A2a adenosine receptor genes. A pseudogene for the A2b receptor was also identified; it exhibited 79% identity to the A2b adenosine receptor cDNA coding sequence and contained multiple deletions, point mutations, and frame shifts and two in-frame stops. These changes would result in the inability to encode a functional receptor. The genomic clones were utilized to localize the A2b receptor to chromosome 17p12 and the A2b pseudogene to chromosome 1q32.
authors.affiliation: Department of Pharmacology, Merck Research Labs, West Point, Pennsylvania 19486, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Jacobson
authors.forename: R G
authors.initials: RG
authors.lastname: Johnson
authors.forename: C J
authors.initials: CJ
authors.lastname: Luneau
authors.forename: C A
authors.initials: CA
authors.lastname: Salvatore
mesh_terms: D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17; D003001:Cloning, Molecular; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D011544:Pseudogenes; D018047:Receptors, Purinergic P1 / Q000235:genetics*
chemical_list: D018047:Receptors, Purinergic P1