title: Peripheral blood CD5+ B cell subset in the remission phase of systemic connective tissue diseases.
abstract: OBJECTIVE
  To get a better insight into the level of circulating CD5+ B cells as related to the systemic connective tissue disease activity.
  
  
  METHODS
  Peripheral blood CD5+CD19+ cells of patients in the remission phase of systemic lupus erythematosus (SLE) (n = 28), Sjögren's syndrome (SS) (n = 20), rheumatoid arthritis (RA) (n = 26), and 19 control healthy subjects were analyzed by 2-color flow cytometry.
  
  
  RESULTS
  In comparison to control group, the patients with SLE had a significant increase in the relative CD19+CD5+ blood cell count (p < 0.0005); this count was also different from the finding in both RA (p < 0.005) and patients with SS (p < 0.05). In contrast, the proportion of B cells expressing CD5 (within an individual B cell population) was significantly increased in all the 3 diseases compared to healthy subjects (SLE, p < 0.0001; SS, p < 0.05; and RA, p < 0.01). In the multivariate discriminant analysis, a discriminant function defined by the CD19+CD5+ subset strongly discriminated SLE, SS and RA from the control, but also SLE from both SS and RA.
  
  
  CONCLUSIONS
  Our findings demonstrated that, in relation to healthy control subjects, the blood CD5+ B subset tended to be elevated in the patients in the remission phase of systemic connective tissue diseases, particularly in SLE.
authors.affiliation: Department of Internal Medicine, Zagreb University Clinical Hospital, Croatia.
authors.forename: J
authors.initials: J
authors.lastname: Markeljević
authors.forename: D
authors.initials: D
authors.lastname: Batinić
authors.forename: B
authors.initials: B
authors.lastname: Uzarević
authors.forename: J
authors.initials: J
authors.lastname: Bozikov
authors.forename: N
authors.initials: N
authors.lastname: Cikes
authors.forename: D
authors.initials: D
authors.lastname: Babić-Naglić
authors.forename: Z
authors.initials: Z
authors.lastname: Horvat
authors.forename: M
authors.initials: M
authors.lastname: Marusić
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000097:blood*; D018941:Antigens, CD19; D000944:Antigens, Differentiation, B-Lymphocyte / Q000097:blood; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D018956:CD5 Antigens; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D012859:Sjogren's Syndrome / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D018941:Antigens, CD19; D000944:Antigens, Differentiation, B-Lymphocyte; D018956:CD5 Antigens