title: Interaction of amiloride with rat parotid muscarinic and alpha-adrenergic receptors.
abstract: 1. In rat parotid acini, amiloride inhibited the secretion of amylase and the efflux of calcium and rubidium in response to carbamylcholine and to norepinephrine. 2. Amiloride competitively inhibited the binding of [3H]N-methylscopolamine and [3H] is thus a competitive antagonist of muscarinic and norepinephrine alpha-adrenergic receptors. 3. Amiloride did not affect the response to substance P with respect to secretion or ion movements. 4. Thus the Na+/H+ antiporter is not involved in the short-term regulation of amylase secretion and calcium and potassium movements in rat parotid gland function.
authors.affiliation: Department of Biochemistry, Université libre de Bruxelles, Belgium.
authors.forename: J P
authors.initials: JP
authors.lastname: Dehaye
authors.forename: V
authors.initials: V
authors.lastname: Verhasselt
mesh_terms: D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology*; D000584:Amiloride / Q000494:pharmacology*; D000681:Amylases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D008297:Male; D018727:Muscarinic Antagonists / Q000494:pharmacology*; D010306:Parotid Gland / Q000187:drug effects* / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013373:Substance P / Q000494:pharmacology
chemical_list: D000317:Adrenergic alpha-Antagonists; D018727:Muscarinic Antagonists; D013373:Substance P; D000584:Amiloride; D011433:Propranolol; D000681:Amylases; D011188:Potassium; D002118:Calcium