Fusion of the EWS gene to a DNA segment from 9q22-31 in a human myxoid chondrosarcoma.
Southern blot analyses revealed a rearrangement of the EWS gene in a skeletal human myxoid chondrosarcoma. Interphase fluorescence in situ hybridization (FISH) studies, using cosmid clones F7 and G9 that flank the EWS locus on 22q12, confirmed the presence of this EWS gene abnormality. Cloning the rearranged EWS DNA fragment and mapping by FISH demonstrated that the EWS gene is joined to DNA sequences localised in 9q22-31. These findings are consistent with previous cytogenetic reports of a recurrent t(9;22)(q22-31;q11-12) in the myxoid variant of chondrosarcoma and reveal involvement of the EWS gene in a fourth type of human sarcoma.
Gill
S
S
Section of Molecular Carcinogenesis, Haddow Laboratories, Sutton, Surrey, United Kingdom.
McManus
A P
AP
Crew
A J
AJ
Benjamin
H
H
Sheer
D
D
Gusterson
B A
BA
Pinkerton
C R
CR
Patel
K
K
Cooper
C S
CS
Shipley
J M
JM
D000328:Adult; D000368:Aged; D002813:Chondrosarcoma / Q000235:genetics* / Q000473:pathology; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000378:metabolism; D005260:Female; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D034802:RNA-Binding Protein EWS; D016601:RNA-Binding Proteins / Q000235:genetics*; D015183:Restriction Mapping; D012261:Ribonucleoproteins / Q000235:genetics*; D014178:Translocation, Genetic
D004273:DNA, Neoplasm; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D034802:RNA-Binding Protein EWS; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins