Functional and physical interactions of components of the yeast mitochondrial inner-membrane import machinery (MIM).
The essential mitochondrial inner-membrane protein, Mim44, is involved in the translocation of preproteins across the mitochondrial inner membrane. Two other putative components of this protein-translocation system are the integral inner-membrane proteins, Mim23 and Mim17. Here, we present genetic evidence for functional co-operation of all three proteins. Furthermore, we show that Mim23 and Mim17 are associated in a protein complex that also contains two proteins of 55 kDa and 20 kDa. We speculate that this subcomplex forms the proteinaceous import channel of the inner-membrane which transiently interacts with a less abundant peripheral complex of Mim44 and mitochondrial heat-shock protein Hsp70.
Blom
J
J
Department of Molecular Cell Biology, BioCentrum Amsterdam, The Netherlands.
Dekker
P J
PJ
Meijer
M
M
D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
D002352:Carrier Proteins; D008565:Membrane Proteins; D033681:Mitochondrial Membrane Transport Proteins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D029701:Saccharomyces cerevisiae Proteins; C077016:TIM44 protein, S cerevisiae