The important role of PKC in controlling polyploidy formation in cultured fibrosarcoma cell line.
Induction of polyploidization by colcemid in cultured fibrosarcoma cells (Meth-A cells) was examined. Activators of protein kinase C (PKC), phorbol 12-myristate 13-acetate (PMA) and ATP, inhibited colcemid-induced polyploidization, but not colcemid-induced cell proliferation cessation. These findings suggest that a down-regulation of PKC activity results in checkpoint "dysfunction" which induces polyploidization and that inhibition of polyploidization induction by PMA and ATP is not a result of the inhibition of colcemid-induced depolymerization of tubulin.
Zong
Z P
ZP
Division of Basic Science, Kanazawa Medical University, Ishikawa, Japan.
Fujikawa-Yamamoto
K
K
Tanino
M
M
Teraoka
K
K
Yamagishi
H
H
Gai
X D
XD
Odashima
S
S
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003703:Demecolcine / Q000037:antagonists & inhibitors / Q000633:toxicity; D004789:Enzyme Activation; D005354:Fibrosarcoma / Q000139:chemically induced / Q000235:genetics / Q000473:pathology*; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D008938:Mitosis / Q000187:drug effects; D009374:Neoplasms, Experimental / Q000139:chemically induced; D011123:Polyploidy; D011493:Protein Kinase C / Q000187:drug effects / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D008748:Methylcholanthrene; D000255:Adenosine Triphosphate; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D003703:Demecolcine