IL-5 enhances in vitro and in vivo antigen-specific IgA production in MHC genetically determined low IL-5 responder mice.
Lymphonode cells from BALB/k mice, but not from BALB/c mice, immunized with picryl chloride (PCl) produce IL-5 when stimulated with the specific antigen in vitro and this correlates with picryl-specific IgA levels in vivo, which are 6 to 10 times higher in BALB/k mice. B lymphocytes from BALB/k mice cultured with PCl-immune T cells from BALB/k produce in vivo anti-PCl-IgA, while B lymphocytes from BALB/c mice, cultured with T cells from BALB/c mice, fail to produce appreciable amounts of anti-PCl IgA, unless IL-5 is added to cultures. B lymphocytes from both strains of mice produce similar amounts of total IgA antibodies when stimulated in vitro with lipopolysaccharide. In vivo administration of IL-5 to BALB/c mice increases significantly PCl-specific IgA levels to those observed in BALB/k mice and a dose-response analysis reveals that 500 units of IL-5 was the minimal effective dose, although a small increase in PCl-specific IgA levels was observed with 100 units of IL-5. Total IgA levels were increased in both strains of mice following in vivo injection of IL-5, but no significant difference in the values was observed. Our results therefore indicate that IL-5 in vivo enhances antigen-specific IgA production in MHC-determined low IL-5 responder mice and suggest an explanation for IgA deficiency in humans.
Dieli
F
F
Institute of General Pathology, University of Palermo, Italy.
Asherson
G L
GL
Sireci
G
G
Lio
D
D
Bonanno
C T
CT
Salerno
A
A
D000818:Animals; D002478:Cells, Cultured; D000939:Epitopes / Q000187:drug effects; D007070:Immunoglobulin A / Q000096:biosynthesis* / Q000187:drug effects*; D007274:Injections, Intraperitoneal; D015848:Interleukin-5 / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008817:Mice, Mutant Strains; D010853:Picryl Chloride / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
D000939:Epitopes; D007070:Immunoglobulin A; D015848:Interleukin-5; D011994:Recombinant Proteins; D010853:Picryl Chloride