A mucin-like glycoprotein identified by MAG (mouse ascites Golgi) antibodies. Menstrual cycle-dependent localization in human endometrium.
Human endometrial glands synthesize and secrete a high molecular weight mucin-like glycoprotein in a menstrual cycle-dependent fashion. A novel moiety within this Golgi-associated glycoprotein is strongly reactive with IgG antibodies in numerous murine ascites, and has been termed MAG (mouse ascites Golgi). Immunohistochemical staining of 201 endometrial biopsies revealed the following patterns: MAG first appeared in the Golgi on cycle day 5, peaked on day 15, was present on the surface of the luminal epithelium between days 17 and 19, and was no longer detectable after day 19. MAG was also present in cervical, prostate, seminal vesicle, and lacrimal glands, pancreatic acinar cells, gall bladder and bile duct epithelium, and certain cells of the salivary and sweat glands. Interestingly, only tissues from blood group A individuals exhibited this staining. As a common link among all these cell types is the expression of mucins, we speculated that the MAG epitope could be a mucin-associated blood group A-related epitope. This hypothesis was tested by absorption experiments with a variety of glycoconjugates and erythrocytes and by immunoblots of MAG-rich material. The absorption studies demonstrated that only type III porcine mucin (< 1% sialic acid) and blood type A or AB erythrocytes were able to absorb the anti-MAG antibody. Inasmuch as N-acetyl-galactosamine alone, the terminal blood group A carbohydrate, did not block MAG antibody binding, the MAG epitope appears to involve N-acetylgalactosamine plus other determinants. Immunoblots of endometrial extracts and saliva from blood type A individuals revealed MAG-reactive material with a molecular weight > 200 kd under reducing conditions. Because the MAG epitope appears on the endometrial surface during the purported implantation window, we speculate that mucin-like epitopes could play a role in the earliest apposition phases of conceptus-endometrial interaction.
Kliman
H J
HJ
Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06520-8023.
Feinberg
R F
RF
Schwartz
L B
LB
Feinman
M A
MA
Lavi
E
E
Meaddough
E L
EL
D000017:ABO Blood-Group System / Q000276:immunology; D000328:Adult; D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D001789:Blood Group Antigens / Q000276:immunology; D002240:Carbohydrate Sequence; D004717:Endometrium / Q000378:metabolism*; D000939:Epitopes / Q000276:immunology; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis* / Q000276:immunology*; D006056:Golgi Apparatus / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D037102:Lectins / Q000276:immunology; D008297:Male; D008597:Menstrual Cycle / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009077:Mucins / Q000096:biosynthesis / Q000276:immunology
D000017:ABO Blood-Group System; D001789:Blood Group Antigens; D000939:Epitopes; D006023:Glycoproteins; D037102:Lectins; D009077:Mucins