Plasmodium falciparum: CD36 dependent cytoadherence or rosetting of infected erythrocytes is modulated by knobs.
A knobless (K-) line of the FCR-3 isolate of Plasmodium falciparum was obtained by gelatin flotation. Immunofluorescent staining and immunoblots indicated that both the K- line and the K+ (knobby) line from which it was derived contained similar forms of potentially adhesive modified band 3 protein. When the K+ and K- lines were assayed for their cytoadherent and rosetting abilities the K+ line showed a high level of CD36 dependent cytoadherence, whereas the K- line demonstrated a marked pH dependent increase in rosetting. Rosetting was inhibited by the addition of peptides based on band 3 motifs, suggesting that cytoadherence and rosetting involve the same adhesin but that the presence of knobs affects whether the adherent preference of the infected erythrocyte is uninfected red cells or endothelial/C32 amelanotic melanoma cells.
Crandall
I
I
Department of Biology, University of California, Riverside 92521, USA.
Guthrie
N
N
Demers
D
D
Sherman
I W
IW
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000502:physiology*; D018955:CD36 Antigens; D002448:Cell Adhesion / Q000502:physiology; D004910:Erythrocyte Membrane / Q000648:ultrastructure; D004912:Erythrocytes / Q000469:parasitology* / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D010641:Phenotype; D010963:Plasmodium falciparum / Q000276:immunology / Q000472:pathogenicity* / Q000502:physiology*; D016024:Receptors, Cytoadhesin / Q000502:physiology*; D012397:Rosette Formation
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018955:CD36 Antigens; D016024:Receptors, Cytoadhesin