title: The functioning of the SRP receptor FtsY in protein-targeting in E. coli is correlated with its ability to bind and hydrolyse GTP.
abstract: In this study, we have established that FtsY, the E. coli homolog of the mammalian signal recognition particle (SRP) receptor, is a GTP-binding protein which displays intrinsic GTPase activity. GTP was found to influence the protease sensitivity of FtsY indicative of a conformational change. FtsY mutated in the 4th GTP-binding consensus element displayed reduced GTP-binding and -hydrolysis which correlated with a reduced ability to interact with SRP. Overexpression of the mutant proteins had a stronger inhibitory effect on protein translocation than overexpression of wild-type FtsY. These observations suggest that in E. coli GTP is important for proper functioning of FtsY in protein-targeting.
authors.affiliation: Department of Molecular Microbiology, Institute of Molecular Biological Sciences, Amsterdam, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Kusters
authors.forename: G
authors.initials: G
authors.lastname: Lentzen
authors.forename: E
authors.initials: E
authors.lastname: Eppens
authors.forename: A
authors.initials: A
authors.lastname: van Geel
authors.forename: C C
authors.initials: CC
authors.lastname: van der Weijden
authors.forename: W
authors.initials: W
authors.lastname: Wintermeyer
authors.forename: J
authors.initials: J
authors.lastname: Luirink
mesh_terms: D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism*; D006868:Hydrolysis; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D018000:Receptors, Peptide / Q000235:genetics / Q000378:metabolism*
chemical_list: D018160:Receptors, Cytoplasmic and Nuclear; D018000:Receptors, Peptide; C036318:signal peptide receptor; D006160:Guanosine Triphosphate; D019204:GTP-Binding Proteins