A study on cell proliferation in cultured human tendons--time dependence, and labeling of 5-bromodeoxyuridine.
Cells which proliferated on cultured human tendon were studied using scanning and transmission electron microscopy, and by immunohistochemical staining. Cell proliferation was continuously observed after 3, 6, 9 and 14 days in culture. Electron microscopy revealed the proliferation and differentiation of fibroblasts and macrophages on the cut surface of the tendon. Immunohistochemical staining by 5-bromodeoxyuridine demonstrated increased cellular activity of the proliferation in the epitenon and endotenon, and also in some mature tenocytes. This activity reached a peak on the 9th day of incubation.
Matsuda
S
S
Department of Orthopaedic Surgery, Shinshu University School of Medicine, Nagano, Japan.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D002648:Child; D003470:Culture Media; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008875:Middle Aged; D016196:S Phase; D013194:Staining and Labeling; D013710:Tendons / Q000166:cytology* / Q000648:ultrastructure; D013997:Time Factors
D003470:Culture Media; D001973:Bromodeoxyuridine