Homologous maturase-like proteins are encoded within the group I introns in different mitochondrial genes specifying Yarrowia lipolytica cytochrome c oxidase subunit 3 and Saccharomyces cerevisiae apocytochrome b.
A mitochondrial cox3 gene in the alkane yeast, Yarrowia lipolytica, encodes a subunit-3 protein of cytochrome c oxidase, and contains a 1044 base-pair-long intron, as compared with the corresponding intronless gene in Saccharomyces cerevisiae. The intron belongs to a group I intron as determined by the cDNA sequence for the splicing sites as well as the predicted RNA secondary structure. Remarkably, this intron could code for a protein of 206 amino-acid residues which showed 63% similarity with an RNA maturase encoded by the second intron of the mitochondrial apocytochrome b gene in S. cerevisiae. Both introns occurred within the conserved exon sequence, 5'-TT(G/C)AGGTGC-3', suggesting the possible transposition of a common ancestral intron.
Matsuoka
M
M
Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.
Matsubara
M
M
Kakehi
M
M
Imanaka
T
T
D000595:Amino Acid Sequence; D001059:Apoproteins / Q000235:genetics*; D001483:Base Sequence; D017124:Conserved Sequence; D003573:Cytochrome b Group / Q000235:genetics*; D045303:Cytochromes b; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics*; D003576:Electron Transport Complex IV / Q000235:genetics*; D004722:Endoribonucleases / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D007438:Introns; D046911:Macromolecular Substances; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009713:Nucleotidyltransferases / Q000235:genetics; D016133:Polymerase Chain Reaction; D012313:RNA / Q000096:biosynthesis; D012326:RNA Splicing; D012331:RNA, Fungal / Q000096:biosynthesis / Q000737:chemistry; D000077278:RNA, Mitochondrial; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D004718:Saccharomycetales / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D015003:Yeasts / Q000201:enzymology / Q000235:genetics*
D001059:Apoproteins; D003573:Cytochrome b Group; D017931:DNA Primers; D004272:DNA, Mitochondrial; D005656:Fungal Proteins; D046911:Macromolecular Substances; D012331:RNA, Fungal; D000077278:RNA, Mitochondrial; D012313:RNA; D045303:Cytochromes b; D003576:Electron Transport Complex IV; D009713:Nucleotidyltransferases; C034038:mRNA maturase; D004722:Endoribonucleases