title: Expression of human cytokeratin 14 in normal, premalignant and malignant oral tissue following isolation by plaque differential hybridisation.
abstract: Differences in gene transcription between RNA samples extracted from oral normal and squamous cell carcinoma (SCC) tissue were examined using the technique of cDNA library differential plaque screening. A differentially expressed transcript was selected on the basis of it being under-expressed in the cancer tissue and was identified, using DNA sequencing, as cytokeratin 14. The level of cytokeratin 14 transcription in RNA samples extracted from a range of oral SCC and normal tissue, as well as "white patch" lesions, was then investigated. Cytokeratin 14 appeared to be significantly under-expressed in oral cancer specimens studied compared to normal and white-patch tissue (P < 0.01). The trend for higher levels of cytokeratin 14 transcription in the dysplastic "white patch" samples compared to that observed for the malignant tissue (P < 0.05) suggests that the decrease in cytokeratin 14 transcription is a late event in the carcinogenic pathway.
authors.affiliation: Division of Dental Surgery, School of Clinical Dentistry, Queen's University Belfast, U.K.
authors.forename: J J
authors.initials: JJ
authors.lastname: Marley
authors.forename: P A
authors.initials: PA
authors.lastname: Robinson
authors.forename: W J
authors.initials: WJ
authors.lastname: Hume
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000737:chemistry*; D015342:DNA Probes; D005260:Female; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D017403:In Situ Hybridization; D007633:Keratins / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009061:Mouth Mucosa / Q000737:chemistry*; D009062:Mouth Neoplasms / Q000737:chemistry*; D011230:Precancerous Conditions / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic
chemical_list: D015342:DNA Probes; D012333:RNA, Messenger; D007633:Keratins