title: Osteogenic protein-1 induces dendritic growth in rat sympathetic neurons.
abstract: Sympathetic neurons from perinatal rat pups extend only a single axon when maintained in culture in the absence of glia and serum. Exposure to recombinant osteogenic protein-1 (OP-1) selectively induces the formation of dendrites that correctly segregate and modify cytoskeletal and membrane proteins and form synaptic contacts of appropriate polarity. OP-1 requires nerve growth factor (NGF) as a cofactor, and, in the presence of optimal concentrations of NGF, OP-1-induced dendritic growth from cultured perinatal neurons is comparable to that observed in situ. Sympathetic neuroblasts that had not formed dendrites in situ also responded to OP-1 in culture, indicating that OP-1 can cause de novo formation as well as regeneration of dendrites. These data imply that specific signals can regulate the development of neuronal shape and polarity.
authors.affiliation: Department of Biology, Canisius College, Buffalo, New York 14208, USA.
authors.forename: P
authors.initials: P
authors.lastname: Lein
authors.forename: M
authors.initials: M
authors.lastname: Johnson
authors.forename: X
authors.initials: X
authors.lastname: Guo
authors.forename: D
authors.initials: D
authors.lastname: Rueger
authors.forename: D
authors.initials: D
authors.lastname: Higgins
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D003712:Dendrites / Q000502:physiology* / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D005728:Ganglia, Sympathetic / Q000648:ultrastructure*; D006133:Growth Substances / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D009414:Nerve Growth Factors / Q000008:administration & dosage / Q000494:pharmacology; D009474:Neurons / Q000648:ultrastructure*; D011506:Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D011994:Recombinant Proteins / Q000494:pharmacology; D013569:Synapses / Q000648:ultrastructure; D016212:Transforming Growth Factor beta
chemical_list: C525819:BMP7 protein, human; C525821:Bmp7 protein, rat; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D009414:Nerve Growth Factors; D011506:Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta