[Inhibition of tumor cell metastasis in chick embryo by beta 1 integrin antisense oligonucleotide].
The antimetastatic effect of integrin beta 1 subunit antisense oligonucleotide on human fibrosarcoma cell (HT-1080) in chick embryo was examined. Phosphorothioate modified antisense oligonucleotide (B1-1) was designed to hybridize specifically to integrin beta 1 subunit mRNA. Pretreatment of HT-1080 cells with B1-1 decreased the metastatic ability in chick embryonic liver and lung, while B1-1 showed no toxicity to HT-1080 nor chick embryo. Western blot analysis showed a reduction of the amount of cell surface integrin beta 1 subunit. B1-1 also inhibited HT-1080 attachment to laminin and fibronectin. These results indicate that the inhibition of tumor cell attachment by antisense oligonucleotide might be a good approach to the prevention of cancer metastasis.
Ninomiya
I
I
Department of Surgery II, School of Medicine, Kanazawa University.
Endo
Y
Y
Yonemura
Y
Y
Sasaki
T
T
D000818:Animals; D002642:Chick Embryo; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019012:Integrin beta1; D016023:Integrins / Q000235:genetics*; D009362:Neoplasm Metastasis / Q000473:pathology*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D012333:RNA, Messenger
D019012:Integrin beta1; D016023:Integrins; D016376:Oligonucleotides, Antisense; D012333:RNA, Messenger