Antigen-specific development of primary and memory T cells in vivo.
The expansion and contraction of specific helper T cells in the draining lymph nodes of normal mice after injection with antigen was followed. T cell receptors from purified primary and memory responder cells had highly restricted junctional regions, indicating antigen-driven selection. Selection for homogeneity in the length of the third complementarity-determining region (CDR3) occurs before selection for some of the characteristic amino acids, indicating the importance of this parameter in T cell receptor recognition. Ultimately, particular T cell receptor sequences come to predominate in the secondary response and others disappear, showing the selective preservation or expansion of specific T cell clones.
McHeyzer-Williams
M G
MG
Department of Microbiology and Immunology, Stanford University School of Medicine, CA 94305, USA.
Davis
M M
MM
D000276:Adjuvants, Immunologic; D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology*; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D018960:Hyaluronan Receptors; D007156:Immunologic Memory / Q000276:immunology*; D019041:L-Selectin; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000096:biosynthesis / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000096:biosynthesis; D016201:Receptors, Lymphocyte Homing / Q000096:biosynthesis; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000941:Antigens; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D016693:Receptors, Antigen, T-Cell, alpha-beta; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D019041:L-Selectin