Analysis of asparagine-linked oligosaccharides by sequential lectin affinity chromatography.
Lectins are proteins that specifically bind to a particular carbohydrate structure. Affinity chromatography with immobilized lectins is a quite effective technique not only for the fractionation of glycoproteins or oligosaccharides but also their structural assessment. In this article, we focus on the separation of glycopeptides and oligosaccharides derived from glycoproteins by affinity chromatography on immobilized lectin columns.
Yamamoto
K
K
Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Tsuji
T
T
Osawa
T
T
D000373:Agglutinins / Q000737:chemistry; D000606:Amino Sugars / Q000737:chemistry; D001216:Asparagine / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D002852:Chromatography, Ion Exchange; D003208:Concanavalin A / Q000737:chemistry; D003911:Dextrans / Q000737:chemistry; D007887:Fabaceae / Q000737:chemistry; D006020:Glycopeptides / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D018532:Pisum sativum / Q000737:chemistry; D037121:Plant Lectins; D010946:Plants, Medicinal; D010947:Plants, Toxic; D012278:Ricinus / Q000737:chemistry; D012794:Sialic Acids / Q000737:chemistry; D014908:Triticum / Q000737:chemistry
D000373:Agglutinins; D000606:Amino Sugars; D002241:Carbohydrates; D003911:Dextrans; D006020:Glycopeptides; D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; D012794:Sialic Acids; D003208:Concanavalin A; D001216:Asparagine; C025614:sephadex; D008358:Mannose