[Spectrophotometric complexes of nucleic acids with pyronine G dyes as a test for radiation damage of DNA].
The spectrophotometric titration curves of the complexes of polynucleotide matrix with pyronine G are highly specific depending on the type and conformation of polyelectrolyte (native and denatured DNA, polyribonucleotides, tRNA). It is found that DNA from the liver of experimental animals of different age, permanent inhabitants of the Chernobyl zone, forms complexes with RNA. A method for testing the defects in a DNA secondary structure have been developed based on comparative analysis of the spectra for the complexes of different nucleic acids with dyes and relative curves of spectrophotometric titration.
Kruglova
E B
EB
Krasnitskaia
A A
AA
Maleev
V Ia
VIa
D000818:Animals; D015415:Biomarkers; D004247:DNA / Q000737:chemistry / Q000528:radiation effects*; D004249:DNA Damage; D008099:Liver / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009696:Nucleic Acids / Q000737:chemistry*; D011210:Power Plants; D011754:Pyronine / Q000737:chemistry*; D012313:RNA / Q000737:chemistry; D011833:Radiation Injuries, Experimental / Q000235:genetics; D018788:Radioactive Hazard Release; D051381:Rats; D014455:Ukraine
D015415:Biomarkers; D009696:Nucleic Acids; D012313:RNA; D004247:DNA; D011754:Pyronine