Infusion of large quantities of autologous blood monocyte-derived macrophages in two cancer patients did not induce increased concentration of IL-6, TNF-alpha, soluble CD14 and nitrate in blood plasma.
In an attempt to increase the number of macrophages available for reinfusion in immunotherapy trials, GM-CSF was injected in vivo to mobilize circulating blood monocytes in 2 cancer patients. Subsequently mononuclear cells were collected by apheresis, cultured in the presence of GM-CSF and activated with IFN-gamma. This procedure resulted in the harvesting of 1.3 to 3.1 x 10(9) (mean 2 x 10(9)) macrophages per apheresis, product which was very well tolerated at autologous reinfusion. These infusions did not induce increased levels of TNF-alpha, IL-6, soluble CD14 nor nitrates in blood plasma (or urine). The lack of TNF-alpha and IL-6 release in blood plasma could explain the good tolerance of these infusions. No in vivo anti-tumoural activity of these high numbers of infused macrophages could be observed.
Lopez
M
M
Inserm U76-CNTS, Paris, France.
Louvet
C
C
Martinache
C
C
Bony
V
V
Scotto
F
F
Barelaud
S
S
Jiang
R
R
Drapier
J C
JC
Smadja
V
V
De Gramont
A
A
D015703:Antigens, CD / Q000097:blood*; D015214:Antigens, Differentiation, Myelomonocytic / Q000097:blood*; D001772:Blood Cell Count / Q000187:drug effects; D016913:Blood Component Transfusion; D001804:Blood Transfusion, Autologous; D002478:Cells, Cultured; D015179:Colorectal Neoplasms / Q000628:therapy*; D016207:Cytokines / Q000097:blood*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D018950:Lipopolysaccharide Receptors; D008264:Macrophages / Q000187:drug effects; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology*; D009566:Nitrates / Q000097:blood*; D012995:Solubility; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D016207:Cytokines; D015850:Interleukin-6; D018950:Lipopolysaccharide Receptors; D009566:Nitrates; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor