Infection with Nippostrongylus brasiliensis induces invasion of mast cell precursors from peripheral blood to small intestine.
Precursors of mast cells were defined as cells that formed mast-cell colonies in methylcellulose culture (CFU-mast). Mononuclear cells (MNC) were obtained from the bone marrow, peripheral blood, and small intestine of Ws/Ws rats with a small deletion at the tyrosine kinase domain of c-kit and of control normal (+/+) rats. In the culture containing concanavalin A-stimulated spleen cell conditioned medium (ConA-SCM) alone, the numbers of mast-cell colonies produced by Ws/Ws MNC were comparable with those of +/+ MNC. In the culture containing both ConA-SCM and stem cell factor (a ligand of c-kit), however, the numbers of mast-cell colonies produced by +/+ blood MNC were 107 times as great as that of Ws/Ws blood MNC. Using this culture condition, we investigated changes in concentration of CFU-mast in the marrow, blood, and intestine of +/+ rats after infection with Nippostrongylus brasiliensis (NB), which induced marked mast-cell accumulation in the small intestine. The concentration of CFU-mast in blood dropped to 21% of preinfection levels 1 week after the NB infection. In contrast, a sevenfold increase of CFU-mast occurred in the small intestine. The proportion of CFU-mast in S phase of the cell cycle remained at low levels in the marrow and blood after NB infection, but it increased significantly in the small intestine. The present result suggests that NB infection induces the invasion of CFU-mast into the intestine from blood and their subsequent proliferation in the tissue site.
Kasugai
T
T
Department of Pathology, Osaka University Medical School, Japan.
Tei
H
H
Okada
M
M
Hirota
S
S
Morimoto
M
M
Yamada
M
M
Nakama
A
A
Arizono
N
N
Kitamura
Y
Y
D000818:Animals; D001772:Blood Cell Count; D001853:Bone Marrow / Q000473:pathology; D002452:Cell Count; D002465:Cell Movement; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D007411:Intestinal Diseases, Parasitic / Q000097:blood / Q000473:pathology*; D007421:Intestine, Small / Q000473:pathology*; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D009559:Nippostrongylus; D011518:Proto-Oncogene Proteins / Q000172:deficiency / Q000235:genetics; D019009:Proto-Oncogene Proteins c-kit; D051381:Rats; D011914:Rats, Inbred BN; D011922:Rats, Mutant Strains; D020794:Receptor Protein-Tyrosine Kinases / Q000172:deficiency / Q000235:genetics; D016184:Receptors, Colony-Stimulating Factor / Q000172:deficiency / Q000235:genetics; D017384:Sequence Deletion; D019089:Stem Cell Factor; D017206:Strongylida Infections / Q000097:blood / Q000473:pathology*; D013601:T-Lymphocytes / Q000378:metabolism
D017077:Culture Media, Conditioned; D016298:Hematopoietic Cell Growth Factors; D011518:Proto-Oncogene Proteins; D016184:Receptors, Colony-Stimulating Factor; D019089:Stem Cell Factor; D003208:Concanavalin A; D019009:Proto-Oncogene Proteins c-kit; D020794:Receptor Protein-Tyrosine Kinases