title: Inhibition of serum-induced proliferation of bovine tracheal smooth muscle cells in culture by heparin and related glycosaminoglycans.
abstract: 1. The effect of heparin and related glycosaminoglycans on bovine airway smooth muscle proliferation has been investigated. 2. Foetal bovine serum stimulated division of bovine trachealis smooth muscle cells in a concentration-dependent fashion at concentrations between 1 and 30%. 3. Heparin (0.1-100 micrograms ml-1), heparan sulphate (0.1-100 micrograms ml-1) and fragmin (0.1-100 micrograms ml-1) inhibited smooth muscle division in a concentration-dependent fashion between 0.1-100 micrograms ml-1. A heparin disaccharide did not exhibit inhibition of division at 100 micrograms ml-1. 4. Dextran sulphate at molecular weights of 5 x 10(3) and 5 x 10(5) concentration-dependently inhibited division between 0.1-100 micrograms ml-1. Dextran without sulphation did not exhibit inhibition of division at 100 micrograms ml-1. 5. The magnitude of inhibition of proliferation did not reach 100% for any compounds examined at concentrations up to 100 micrograms ml-1 during incubations for 5 and 14 days. IC50 values for inhibition of proliferation ranged between 1-5 micrograms ml-1. 6. These findings suggest that heparin and related glycosaminoglycans inhibit bovine airway smooth muscle cell division.
authors.affiliation: Sackler Institute of Pulmonary Pharmacology, Department of Thoracic Medicine, Kings College School of Medicine and Dentistry, London.
authors.forename: S A
authors.initials: SA
authors.lastname: Kilfeather
authors.forename: S
authors.initials: S
authors.lastname: Tagoe
authors.forename: A C
authors.initials: AC
authors.lastname: Perez
authors.forename: K
authors.initials: K
authors.lastname: Okona-Mensa
authors.forename: R
authors.initials: R
authors.lastname: Matin
authors.forename: C P
authors.initials: CP
authors.lastname: Page
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology; D002417:Cattle; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division; D002478:Cells, Cultured / Q000187:drug effects; D003911:Dextrans / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000187:drug effects*; D014132:Trachea / Q000187:drug effects*
chemical_list: D001798:Blood Proteins; D003911:Dextrans; D006025:Glycosaminoglycans; D006493:Heparin