Increased DT-diaphorase activity in transformed and tumorigenic pancreatic acinar cells.
Pancreatic acinar cells from rats treated in vitro with 5-azacytidine and/or transfected with an activated c-H-ras demonstrated transformation and tumorigenic phenotypes. DT-diaphorase (NAD(P)H:quinone oxidoreductase) activity was determined in these non-tumorigenic (3AP) and tumorigenic cells (T3AP and T5AM). T5AM cells were those treated with 5-azacytidine and further treated with N'-methyl-N'-nitro-nitrosoguanidine. Higher levels of enzyme activity were found in transformed cells when compared to that in control cells (> 15-fold, 3AP cells; > 40-fold, T3AP cells; > 20-fold T5AM cells). In contrast, NADPH-cytochrome c reductase activity was decreased in transformed cells (> 10-fold, 3AP cells; > 20-fold, T3AP cells; > 10-fold, T5AM cells). These studies demonstrated that pancreatic acinar cells are capable of undergoing alterations in enzyme activity patterns when transformed and that DT-diaphorase may be a good marker for malignant transformation.
Hammons
G J
GJ
Office of Research, National Center for Toxicological Research, Jefferson, AR 72079, USA.
Warren
G J
GJ
Blann
E
E
Nichols
J
J
Lyn-Cook
B D
BD
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D001374:Azacitidine / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002478:Cells, Cultured; D011905:Genes, ras; D066298:In Vitro Techniques; D008745:Methylation; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344
D000964:Antimetabolites, Antineoplastic; D016660:NAD(P)H Dehydrogenase (Quinone); D009245:NADH Dehydrogenase; D001374:Azacitidine