title: The nature of the epithelium in acquired cholesteatoma: Part 3--Cytokeratin patterns in aural epithelial and cholesteatoma cells grown in cell culture.
abstract: The nature and origin of the epithelial layers in acquired cholesteatoma is still unclear. Although previous morphological studies comparing external meatal and cholesteatoma epithelium have shown no significant difference, bone resorption is generally much more severe with cholesteatoma than with chronic otitis media without cholesteatoma. It is possible that cholesteatoma epithelium has undergone transformation leading to its enhanced bone destroying role. In this study the cytokeratin patterns of aural and cholesteatoma epithelia grown in cell culture were compared using monoclonal antibodies. No significant difference in staining patterns were found suggesting that there has been no change in cell phenotype which maintains that of external auditory meatus epithelium. This study therefore supports the immigration theory of cholesteatoma genesis.
authors.affiliation: Department of Otolaryngology, Kettering General Hospital, UK.
authors.forename: R J
authors.initials: RJ
authors.lastname: Lee
authors.forename: C
authors.initials: C
authors.lastname: Sidey
authors.forename: A A
authors.initials: AA
authors.lastname: Narula
authors.forename: R F
authors.initials: RF
authors.lastname: James
mesh_terms: D000911:Antibodies, Monoclonal; D001862:Bone Resorption / Q000378:metabolism / Q000473:pathology; D002465:Cell Movement; D002478:Cells, Cultured; D018424:Cholesteatoma, Middle Ear / Q000378:metabolism* / Q000473:pathology; D002908:Chronic Disease; D004424:Ear Canal / Q000378:metabolism / Q000473:pathology; D004848:Epithelium / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D010033:Otitis Media / Q000378:metabolism / Q000473:pathology; D010641:Phenotype; D012867:Skin / Q000378:metabolism / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D007633:Keratins