title: Activity of the yeast FLP recombinase in Arabidopsis.
abstract: The coding sequence for FLP recombinase, originally from the 2 mu plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked by a pair of FRT target sites in inverted repeat. FLP-dependent Gus activity was observed in both transient assays and transgenic plants. The FLP system will be useful for a variety of in planta genetic manipulations.
authors.affiliation: Department of Biology, Massachusetts Institute of Technology, Cambridge 02139-4307, USA.
authors.forename: R V
authors.initials: RV
authors.lastname: Sonti
authors.forename: A F
authors.initials: AF
authors.lastname: Tissier
authors.forename: D
authors.initials: D
authors.lastname: Wong
authors.forename: J F
authors.initials: JF
authors.lastname: Viret
authors.forename: E R
authors.initials: ER
authors.lastname: Signer
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D007446:Chromosome Inversion; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology
chemical_list: D005656:Fungal Proteins; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase