The (2'-5')oligoadenylate synthetase is present in the lowest multicellular organisms, the marine sponges. Demonstration of the existence and identification of its reaction products.
We have proved the presence of (2'-5')oligoadenylates [(2'-5')An] and oligoadenylate synthetase [(2'-5')An synthetase] in the marine sponge Geodia cydonium. (2'-5')An isolated from sponge crude extract competed with authentic (2'-5')An for binding to polyclonal antiserum against (2'-5')An. HPLC analysis revealed the presence of nucleotides eluting with molecular markers for (2'-5')A oligomers. The biological activity of sponge (2'-5')An was demonstrated by inhibiting the protein biosynthesis in rabbit reticulocyte lysate. The activity of the (2'-5')An synthetase, present in crude sponge extract, was found to be high compared to that in mammalian interferon-treated cell extract. The (2'-5')An synthetase from sponge extract binds to poly(I).poly(C) as does the mammalian enzyme. Western blot analysis with antibodies to recombinant rat 43-kDa (2'-5')An synthetase revealed in sponge immunologically related proteins with molecular masses of approximately 110, 65, 61 and 34 kDa. We conclude, that the (2'-5')An system has evolved from receptors and enzymes involved in cell adhesion and/or growth control.
Kuusksalu
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Institute of Chemical Physics and Biophysics, Tallinn, Estonia.
Pihlak
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Müller
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Kelve
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D015088:2',5'-Oligoadenylate Synthetase / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D000227:Adenine Nucleotides / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D005075:Biological Evolution; D001798:Blood Proteins / Q000096:biosynthesis; D015153:Blotting, Western; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D007120:Immunochemistry; D066298:In Vitro Techniques; D051379:Mice; D008970:Molecular Weight; D009843:Oligoribonucleotides / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D011161:Porifera / Q000201:enzymology* / Q000378:metabolism; D011500:Protein Synthesis Inhibitors / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism; D013045:Species Specificity
D000227:Adenine Nucleotides; D001798:Blood Proteins; D009843:Oligoribonucleotides; D011500:Protein Synthesis Inhibitors; C023505:2',5'-oligoadenylate; D015088:2',5'-Oligoadenylate Synthetase