The fasciovascular flap: a new vehicle for islet transplantation.
In order to determine whether pancreatic islets could be neovascularized by a fasciovascular flap (FVP), islet transplant studies were conducted in Lewis rats. Islets from two donors were isolated by collagenase digestion and discontinuous gradient centrifugation on Ficoll. These islets were injected in syngeneic recipients either into random groin SC fat as a control, or into a flap composed of fascia and fat elevated from the groin based on the superficial inferior epigastric vessels. After two wk, islet viability was assessed by histological analysis. The degree of neovascularization of the islet tissue was evaluated with India ink injection through the vascular pedicle. Whereas control islets degenerated and did not show clear signs of neovascularization, FVP-islets showed rich neovascularization and viability as a large sheet of islet clusters. These results have demonstrated that the FVP-flap is a novel recipient site which can support a large quantity of islet tissue. This model constitutes a unique neo-endocrine pancreas flap, which can be subsequently transplanted at will to transfer the established neo-endocrine pancreas to a desired site using microvascular surgical technique.
Borud
L J
LJ
Department of Surgery, University of California, Los Angeles.
Shaw
W W
WW
Passaro
E
E
Brunicardi
F C
FC
Mullen
Y
Y
D000273:Adipose Tissue; D000818:Animals; D001012:Aorta, Abdominal; D002244:Carbon; D002469:Cell Separation; D002470:Cell Survival; D002498:Centrifugation; D004396:Coloring Agents; D005362:Ficoll; D007515:Islets of Langerhans / Q000166:cytology; D016381:Islets of Langerhans Transplantation / Q000379:methods* / Q000473:pathology / Q000502:physiology; D051381:Rats; D011917:Rats, Inbred Lew; D013194:Staining and Labeling; D013270:Stomach / Q000098:blood supply; D013524:Surgical Flaps / Q000379:methods; D016042:Transplantation, Heterotopic; D014185:Transplantation, Isogeneic
D004396:Coloring Agents; C028433:chinese ink; D005362:Ficoll; D002244:Carbon