title: Evaluation of different serological tests for detection of antibodies against Serpulina hyodysenteriae in pig sera.
abstract: Swine dysentery is a mucohemorrhagic diarrheal disease caused by S. hyodysenteriae. The detection of asymptomatic carriers in herds is possible by serological tests. However, cross-reactions between S. hyodysenteriae and S. innocens pose a major problem in serological diagnosis. Several serological tests were evaluated for detection of antibodies to S. hyodysenteriae such as: indirect hemagglutination, passive hemolysis, conglutination and microagglutination tests. Among the tests used, only the microagglutination test was able to detect antibodies to S. hyodysenteriae. 70 to 95% of the pigs were invariably seropositive in a single dilution of 1:10 in actively infected herds whereas the number of seropositives did not exceed 10% in presumably non-infected herds. The test was found to be simple, and reliable to be used with confidence for detection of herd infection using boiled cell suspension as an antigen.
authors.affiliation: Groupe de Recherche sur les maladies infectieuses du porc (GREMIP), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.
authors.forename: A T
authors.initials: AT
authors.lastname: Diarra
authors.forename: M
authors.initials: M
authors.lastname: Achacha
authors.forename: K R
authors.initials: KR
authors.lastname: Mittal
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D017401:Brachyspira hyodysenteriae / Q000276:immunology*; D003168:Complement Fixation Tests; D003429:Cross Reactions; D004403:Dysentery / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D005069:Evaluation Studies as Topic; D006386:Hemagglutination Tests / Q000379:methods; D006461:Hemolysis; D012698:Serologic Tests / Q000379:methods*; D013145:Spirochaetales Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial