title: [32P]orthophosphate and [35S]methionine label separate pools of neurofilaments with markedly different axonal transport kinetics in mouse retinal ganglion cells in vivo.
abstract: Newly synthesized neurofilament proteins become highly phosphorylated within axons. Within 2 days after intravitreously injecting normal adult mice with [32P]orthophosphate, we observed that neurofilaments along the entire length of optic axons were radiolabeled by a soluble 32P-carrier that was axonally transported faster than neurofilaments. 32P-incorporation into neurofilament proteins synthesized at the time of injection was comparatively low and minimally influenced the labeling pattern along axons. 32P-incorporation into axonal neurofilaments was considerably higher in the middle region of the optic axons. This characteristic non-uniform distribution of radiolabel remained nearly unchanged for at least 22 days. During this interval, less than 10% of the total 32P-labeled neurofilaments redistributed from the optic nerve to the optic tract. By contrast, newly synthesized neurofilaments were selectively pulse-labeled in ganglion cell bodies by intravitreous injection of [35S]methionine and about 60% of this pool translocated by slow axoplasmic transport to the optic tract during the same time interval. These findings indicate that the steady-state or resident pool of neurofilaments in axons is not identical to the newly synthesized neurofilament pool, the major portion of which moves at the slowest rate of axoplasmic transport. Taken together with earlier studies, these results support the idea that, depending in part on their phosphorylation state, transported neurofilaments can interact for short or very long periods with a stationary but dynamic neurofilament lattice in axons.
authors.affiliation: Laboratories for Molecular Neuroscience, McLean Hospital, Belmont, Massachusetts 02178.
authors.forename: R A
authors.initials: RA
authors.lastname: Nixon
authors.forename: S E
authors.initials: SE
authors.lastname: Lewis
authors.forename: M
authors.initials: M
authors.lastname: Mercken
authors.forename: R K
authors.initials: RK
authors.lastname: Sihag
mesh_terms: D000818:Animals; D001345:Autoradiography; D001370:Axonal Transport; D001369:Axons / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D008715:Methionine / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D016900:Neurofilament Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D010710:Phosphates / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D012165:Retinal Ganglion Cells / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D013997:Time Factors
chemical_list: D016900:Neurofilament Proteins; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D013462:Sulfur Radioisotopes; D008715:Methionine