Differential responses of hormone-sensitive lipase gene to nutritional transition in adipose tissue, liver, and skeletal muscle of pigs.
To study the regulation of hormone-sensitive lipase gene in pigs, we amplified and sequenced partial porcine hormone-sensitive lipase cDNA. Nucleotide analysis indicated that porcine hormone-sensitive lipase cDNA was 86% homologous with the rat. In agreement with the rat, a 3.3 kb mRNA transcript was detected in adipose tissue but not in skeletal muscle of pigs by Northern hybridization. With more sensitive PCR method, hormone-sensitive lipase mRNA was found in adipose tissue, liver, heart, skeletal muscle, testis, and spleen. The gene expression in adipose tissue and liver was elevated after 2 days of fasting, and refeeding for another 2 days decreased the mRNA abundance. In contrast, the levels in skeletal muscle were not altered during identical nutritional transition. Combined evidences suggest that differential control may occur in porcine hormone-sensitive lipase gene in a tissue-specific fashion.
Liu
C Y
CY
Department of Comparative Medicine, Pig Research Institute Taiwan, Miaoli, R.O.C.
Liang
L C
LC
Chang
L C
LC
D000273:Adipose Tissue / Q000201:enzymology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004032:Diet; D015971:Gene Expression Regulation, Enzymologic; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D002787:Sterol Esterase / Q000096:biosynthesis / Q000235:genetics*; D013552:Swine
D012333:RNA, Messenger; D002787:Sterol Esterase