title: Identification of tumour-specific translocations in archival material.
abstract: A reverse transcriptase nested polymerase reaction (RT-nPCR) method has been developed to detect the presence of a tumour-specific translocation in archival formalin-fixed, paraffin-embedded tissue. This technique can be applied to the large numbers of tumours in pathology departments' archives, allowing correlation of translocations with morphological diagnosis, response to therapy, and survival.
authors.affiliation: Department of Pathology, Cambridge University, Addenbrookes Hospital, U.K.
authors.forename: G H
authors.initials: GH
authors.lastname: Williams
authors.forename: E D
authors.initials: ED
authors.lastname: Williams
mesh_terms: D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis; D002291:Carcinoma, Papillary / Q000235:genetics* / Q000473:pathology; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase; D015996:Survival Rate; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D014178:Translocation, Genetic; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D017931:DNA Primers; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase