title: The characterization of chromosomal rearrangements by a combined micro-FISH approach in a patient with myelodysplastic syndrome.
abstract: We report the application of a combined strategy: chromosome microdissection, degenerate oligonucleotide primed-PCR, and reverse chromosome painting (micro-FISH), as well as forward chromosome painting, for the characterization of chromosomal rearrangements in a MDS patient with the karyotype 46,XX, -11, +r analyzed by G-banding. The karyotype was refined as 46,XX,der(2)t(2;11) (q35;?p13),der(11)dic r(11)(:p13-->q13::p13-->q13:). Our study demonstrated that the chromosome composition of a neoplasia can be identified more systematically and accurately using these combined molecular cytogenetic approaches. The DOP-PCR methodology modified is suitable for the practical application of micro-FISH on specimens prepared for routine banding analysis.
authors.affiliation: Department of Pediatrics, University of Rochester School of Medicine, New York, USA.
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: E
authors.initials: E
authors.lastname: Cedrone
authors.forename: M
authors.initials: M
authors.lastname: Roberts
authors.forename: G
authors.initials: G
authors.lastname: Wu
authors.forename: S
authors.initials: S
authors.lastname: Gershagen
authors.forename: N
authors.initials: N
authors.lastname: Wang
mesh_terms: D000368:Aged; D001483:Base Sequence; D002869:Chromosome Aberrations / Q000235:genetics; D005260:Female; D015321:Gene Rearrangement / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008969:Molecular Sequence Data; D009190:Myelodysplastic Syndromes / Q000235:genetics*