Plasma exudation in conscious dogs with experimental heart failure.
The purpose of this study was to determine the effects of bradykinin (BK), substance P (SP) and histamine on plasma exudation in the skin of conscious dogs with and without pacing-induced heart failure. We also determined the role tissue angiotensin I-converting enzyme (ACE) and neutral endopeptidase (NEP) play in modulating these responses. We found that intradermal injection of BK, SP and histamine induced a significant, concentration-dependent Evans blue exudation in normal dogs (p < 0.05). Bradykinin-induced responses were significantly potentiated by captopril (p < 0.05). In contrast, phosphoramidon potentiated BK-induced responses only at low concentrations of BK. Both captopril and phosphoramidon had no significant effects on SP- and histamine-induced Evans blue exudation. BK- and SP-induced responses were significantly attenuated, whereas histamine-induced Evans blue exudation was significantly potentiated in dogs with heart failure. We conclude that heart failure is associated with attenuation of BK- and SP-, but not histamine-induced plasma exudation in the peripheral microcirculation and that these responses are not modulated by tissue ACE and NEP.
Rubinstein
I
I
Department of Medicine, University of Illinois at Chicago.
Müns
G
G
Zucker
I H
IH
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002216:Captopril / Q000494:pharmacology; D004195:Disease Models, Animal; D004285:Dogs; D004347:Drug Interactions; D005070:Evans Blue; D005122:Exudates and Transudates / Q000187:drug effects; D006020:Glycopeptides / Q000494:pharmacology; D006333:Heart Failure / Q000503:physiopathology*; D006632:Histamine / Q000494:pharmacology*; D015260:Neprilysin / Q000037:antagonists & inhibitors / Q000502:physiology; D007703:Peptidyl-Dipeptidase A / Q000502:physiology; D012867:Skin / Q000098:blood supply*; D013373:Substance P / Q000494:pharmacology*
D006020:Glycopeptides; D013373:Substance P; D005070:Evans Blue; D006632:Histamine; D002216:Captopril; D007703:Peptidyl-Dipeptidase A; D015260:Neprilysin; D001920:Bradykinin; C008890:phosphoramidon