Chitinolytic activity of the anaerobic rumen fungus Piromyces communis OTS1.
The anaerobic rumen fungus, Piromyces communis OTS1, was isolated from a fistulated goat. Its chitinolytic activity in the supernatant of media containing different types of chitin was studied. The fungus grew well in our basal medium, with and without colloidal chitin and chitin powder. N-Acetyl-beta-glucosaminidase activity was not detected in any of the culture media. Chitinase activity, however, was detected in the basal medium with and without colloidal chitin and chitin powder. The extracellular chitinase concentrated from the fungal culture's supernatant by ammonium sulfate (80% saturation) showed highest activity at 40 degrees C and at pH 5.5. In the other cell fractions of P. communis OTS1, N-acetyl-beta-glucosaminidase was not detected, but chitinase activity was detected by 4-methylumbelliferyl reagents. Thus, it was found that the rumen fungus P. communis OTS1 has chitinase activity. Chitinases from the extracellular, cytosolic, and the microsomal fractions were mainly of the endo type of chitinase activity.
Sakurada
M
M
Laboratory of Animal Nutrition and Biochemistry, Faculty of Agriculture, Miyazaki University, Japan.
Morgavi
D P
DP
Tomita
Y
Y
Onodera
R
R
D000818:Animals; D002688:Chitinases / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005658:Fungi / Q000201:enzymology*; D006041:Goats; D006863:Hydrogen-Ion Concentration; D012417:Rumen / Q000382:microbiology*; D013347:Subcellular Fractions / Q000201:enzymology; D013696:Temperature
D003470:Culture Media; D002688:Chitinases