title: Usefulness of the AMeX method for immunostaining with antikeratin antibodies.
abstract: We performed an immunohistochemical study with 11 antikeratin antibodies using the newly developed AMeX (acetone-methylbenzoate-xylene) tissue processing method. Specimens processed with this method showed almost as good preservation and morphological detail as routinely processed, formalin-fixed and paraffin-embedded tissue specimens, and as good preservation of antigenicity as fresh frozen tissue specimens. Thus, we propose the wide use of this method in dermatology.
authors.affiliation: Department of Dermatology, Yamagata University School of Medicine, Japan.
authors.forename: S I
authors.initials: SI
authors.lastname: Ansai
authors.forename: S
authors.initials: S
authors.lastname: Koseki
authors.forename: Y
authors.initials: Y
authors.lastname: Hozumi
authors.forename: S
authors.initials: S
authors.lastname: Kondo
mesh_terms: D000096:Acetone; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001565:Benzoates; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000276:immunology*; D008297:Male; D012867:Skin; D014021:Tissue Preservation / Q000379:methods*; D014992:Xylenes
chemical_list: D000911:Antibodies, Monoclonal; D001565:Benzoates; D014992:Xylenes; D000096:Acetone; C044605:methyl benzoate; D007633:Keratins