Extracellular matrix proteins inhibit proliferation, upregulate migration and induce morphological changes in human glioma cell lines.
The influence of an artificial basement membrane (BM), Matrigel, and four individual extracellular matrix proteins, fibronectin, laminin, collagen I and vitronectin, on cell proliferation, morphology and migration was assessed in four glioma cell lines. Matrigel and individual BM proteins differentially inhibited cell proliferation of all cell lines studied. In addition, Matrigel was found to induce extensive morphological changes in glioma cells. Polycarbonate filters, of 8-microns porosity in modified Boyden chambers, were used to assess the chemoattraction activity of Matrigel and the individual proteins on glioma cells. All these components were found to stimulate cell migration, albeit to different extents but laminin proved to be the most effective chemoattractant for glioma cells in vitro. These data suggest that basement membrane proteins may inhibit proliferation and stimulate migration in order to facilitate invasion.
Koochekpour
S
S
Department of Neuropathology, Institute of Psychiatry, London, U.K.
Merzak
A
A
Pilkington
G J
GJ
D002455:Cell Division / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D003094:Collagen / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology; D005910:Glioma / Q000473:pathology*; D006023:Glycoproteins / Q000494:pharmacology; D006801:Humans; D007797:Laminin / Q000494:pharmacology*; D011509:Proteoglycans / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D019096:Vitronectin
D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D006023:Glycoproteins; D007797:Laminin; D011509:Proteoglycans; D019096:Vitronectin; C059485:matrigel; D003094:Collagen