Structure of the complex between the Fab fragment of a neutralizing antibody for type 1 poliovirus and its viral epitope.
The crystal structure of the complex between the Fab fragment of C3, a neutralizing antibody for poliovirus, and a peptide corresponding to the viral epitope has been determined at 3.0 A resolution. Although this antibody was originally raised to heat inactivated (noninfectious) virus particles, it strongly neutralizes the Mahoney strain of type 1 poliovirus. Eleven peptide residues are well-defined in the electron-density map and form two type I beta-turns in series. At the carboxyl end, the peptide is bound snugly in the antibody-combining site and adopts a conformation that differs significantly from the structure of the corresponding residues in the virus. Structural comparisons between the peptide in the complex and the viral epitope suggests that on binding to infectious virions, this antibody may induce structural changes important for neutralization.
Wien
M W
MW
Committee for Higher Degrees in Biophysics, Harvard University, Cambridge, Massachusetts 02138, USA.
Filman
D J
DJ
Stura
E A
EA
Guillot
S
S
Delpeyroux
F
F
Crainic
R
R
Hogle
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000235:genetics; D000914:Antibodies, Viral / Q000737:chemistry* / Q000235:genetics; D000936:Antigen-Antibody Complex / Q000737:chemistry*; D000956:Antigens, Viral / Q000737:chemistry*; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D000939:Epitopes / Q000737:chemistry; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000235:genetics; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009500:Neutralization Tests; D017955:Poliovirus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D018076:DNA, Complementary; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments