title: The neuritogenic effect of myenteric plexus on striatal neurones in co-culture involves nitric oxide.
abstract: We reported previously that myenteric plexus explants promoted striatal neurite elongation in co-culture and that this effect was abolished by tetrodotoxin (TTX). Here we demonstrate that the nitric oxide synthase blocker N-nitro-L-arginine methyl ester significantly reduced the neuritogenic effect of the myenteric plexus whereas the nitric oxide donor, sodium nitroprusside (SNP), partially reversed the blocking effect of TTX. 2-Chloroadenosine (2-CA), a stable analogue of adenosine, which is produced following release of ATP from enteric neurones, further enhanced the effect of SNP. Basic fibroblast growth factor or neurotrophin-3 in combination with 2-CA and SNP were only marginally neuritogenic in striatal cultures alone. These results suggest that NO is involved in the trophic effects of myenteric plexus explants on striatal neurones.
authors.affiliation: Department of Anatomy and Developmental Biology, University College London, UK.
authors.forename: V H
authors.initials: VH
authors.lastname: Höpker
authors.forename: M J
authors.initials: MJ
authors.lastname: Saffrey
authors.forename: G
authors.initials: G
authors.lastname: Burnstock
mesh_terms: D015762:2-Chloroadenosine / Q000494:pharmacology; D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors*; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002478:Cells, Cultured; D003342:Corpus Striatum / Q000166:cytology* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D009197:Myenteric Plexus / Q000166:cytology* / Q000378:metabolism; D019331:NG-Nitroarginine Methyl Ester; D016501:Neurites / Q000187:drug effects* / Q000502:physiology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019001:Nitric Oxide Synthase; D051381:Rats; D017207:Rats, Sprague-Dawley; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D015762:2-Chloroadenosine; D009569:Nitric Oxide; D013779:Tetrodotoxin; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D019331:NG-Nitroarginine Methyl Ester