title: Eosinophil granule proteins in cardiopulmonary bypass with and without heparin coating.
abstract: Extracorporeal circulation with exposure of blood to foreign surfaces causes activation of different defense systems, eg, white cells. Several potent mediators are released into plasma, capable of causing harmful effects to different organs, contributing to postoperative morbidity after operations using cardiopulmonary bypass. The eosinophil granulocyte has not previously been investigated in this respect. We studied two of its activation products, eosinophil cationic protein and eosinophil protein X in coronary bypass patients. In 17 control patients, plasma levels of eosinophil cationic protein and eosinophil protein X increased considerably during cardiopulmonary bypass. In 19 patients with heparin-coated cardiopulmonary bypass equipment the levels were significantly reduced, indicating improved biocompatibility of the cardiopulmonary bypass circuit. The heparin-coated surface causes less activation of eosinophils; also released eosinophil cationic protein is bound to the heparinized surface.
authors.affiliation: Department of Thoracic Surgery, University Hospital, Uppsala, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Nilsson
authors.forename: C
authors.initials: C
authors.lastname: Peterson
authors.forename: P
authors.initials: P
authors.lastname: Venge
authors.forename: J W
authors.initials: JW
authors.lastname: Borowiec
authors.forename: S
authors.initials: S
authors.lastname: Thelin
mesh_terms: D001798:Blood Proteins / Q000187:drug effects* / Q000378:metabolism; D002315:Cardiopulmonary Bypass / Q000379:methods*; D002846:Chromatography, Affinity; D003911:Dextrans; D047091:Eosinophil Granule Proteins; D047208:Eosinophil-Derived Neurotoxin; D005782:Gels; D006493:Heparin / Q000008:administration & dosage*; D006801:Humans; D008297:Male; D012260:Ribonucleases; D013997:Time Factors; D014914:Whole Blood Coagulation Time
chemical_list: D001798:Blood Proteins; D003911:Dextrans; D047091:Eosinophil Granule Proteins; D005782:Gels; D006493:Heparin; C025614:sephadex; D047208:Eosinophil-Derived Neurotoxin; D012260:Ribonucleases