Fast inducible repair of microinjected UV-irradiated SV40 DNA in monkey kidney cells.
In monkey kidney cells (TC-7), microinjected with UV-irradiated (103-362 J/m2) SV40 DNA, the expression of viral antigens decreases in a UV-dose-dependent manner and the viral genes are not repaired constitutively. When the viral DNA is microinjected 4 h after UV-irradiation (40 J/m2) of host cells, the expression of viral antigens is restored in all cells. The time course of restoration of viral gene expression function shows that in UV-irradiated cells the repair is induced rapidly and fully within 2 h and the induced state is maintained for 24 h. Intact viral DNA molecules, microinjected during the period of induction of cellular UV repair, are expressed less efficiently than UV-irradiated viral genomes.
Ghaskadbi
S
S
Department of Zoology, University of Poona, Pune, India.
Apte
V
V
Modak
S P
SP
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis* / Q000276:immunology; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology / Q000378:metabolism; D004249:DNA Damage; D004260:DNA Repair; D004267:DNA Viruses / Q000378:metabolism* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005455:Fluorescent Antibody Technique; D015967:Gene Expression Regulation, Viral; D016679:Genome, Viral; D000882:Haplorhini; D006918:Hydroxyurea / Q000494:pharmacology; D007668:Kidney; D008845:Microinjections; D013539:Simian virus 40 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D014466:Ultraviolet Rays
D000956:Antigens, Viral; D006918:Hydroxyurea