Non-sialate inhibitor of influenza A/WSN/33 neuraminidase.
An N1 strain of influenza A virus neuraminidase (A/WSN/33 NA) was purified and used to screen for inhibitors. As a result, a well-known tuberculostatic, 4'-formylacetanilide thiosemicarbazone (or thiacetazone), was identified. Thiacetazone is a non-sialate compound and inhibits the enzyme in a noncompetitive manner with respect to the substrate sialic acid. Mechanistic studies indicate that the inhibition was due to the competition of thiacetazone with Ca2+, which maintains N1 neuraminidase in an active conformation. The Ki for the inhibition was estimated to be about 4 microM. Equilibrium exchange experiments revealed that when purified A/WSN/33 NA was incubated with 5 microM 45CaCl2, 2 mol of 45Ca2+ ion was exchanged into each mole of NA tetramer and subsequently displaced from the enzyme upon the introduction of the inhibitor. Inhibition of plaque formation by thiacetazone in an MDCK cell culture that had been infected with the influenza A/WSN/33 virus was demonstrated. Thiacetazone was highly specific for A/WSN/33 neuraminidase, since little effect was noted when it was tested against NAs from the other strains of influenza virus or from bacteria. This compound might represent a group of non-sialate inhibitors of influenza NA that bind to a noncatalytic or an allosteric site on the enzyme.
Wu
J C
JC
Boehringer Ingelheim Pharmaceuticals, Inc., Ridgefield, Connecticut 06877, USA.
Peet
G W
GW
Coutts
S J
SJ
Eckner
R J
RJ
Griffin
J A
JA
Farina
P R
PR
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004285:Dogs; D009980:Influenza A virus / Q000201:enzymology* / Q000502:physiology; D009439:Neuraminidase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013828:Thioacetazone / Q000494:pharmacology*; D010948:Viral Plaque Assay
D009439:Neuraminidase; D013828:Thioacetazone; D002118:Calcium