title: Gene expression and phosphorylation of mouse osteopontin.
abstract: Osteopontin is expressed in many different cell types and has been proposed to play several functions. Distinct forms of the protein have been detected. Various tissues and cell lines from mouse, however, exhibit two classes of transcripts with different 5'-untranslated ends but with an identical coding region (exons II through VII). These transcripts do not arise from the alternative splicing of coding exons. These results suggest that posttranslational modifications of osteopontin, such as phosphorylation, are a major mechanism to generate different forms of the protein. Mouse osteopontin was expressed in E. coli and used as a model to study its phosphorylation.
authors.affiliation: Department of Orthopaedic Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: R A
authors.initials: RA
authors.lastname: Saavedra
authors.forename: S K
authors.initials: SK
authors.lastname: Kimbro
authors.forename: D N
authors.initials: DN
authors.lastname: Stern
authors.forename: J
authors.initials: J
authors.lastname: Schnuer
authors.forename: S
authors.initials: S
authors.lastname: Ashkar
authors.forename: M J
authors.initials: MJ
authors.lastname: Glimcher
authors.forename: C I
authors.initials: CI
authors.lastname: Ljubetic
mesh_terms: D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D017931:DNA Primers / Q000737:chemistry; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008969:Molecular Sequence Data; D053495:Osteopontin; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine; D012333:RNA, Messenger / Q000235:genetics; D012795:Sialoglycoproteins / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D017931:DNA Primers; D010750:Phosphoproteins; D012333:RNA, Messenger; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D053495:Osteopontin; D019000:Phosphotyrosine; D014443:Tyrosine