Combination effects of gamma-interferon with pinyanmycin or psoralen on tongue cancer HSC3 cell line in vitro and in vivo.
The inhibitory effects of gamma-interferon (gamma-IFN), pinyanmycin (PYM), and psoralen (PSO) on human tongue cancer HSC3 cells were tested with MTT assay (tetrazolium-based colorimetric assay). The IC50 (drug concentration which causes 50% inhibition of the cell line) values of gamma-IFN, PYM and PSO were 371,535 U/ml, 224 ng/ml and 25,119 ng/ml, respectively. The CI50 (combination index) values of the combinations of PYM with gamma-IFN at the concentrations of 10(2), 10(3), 10(4) and 10(5) U/ml were 0.12, 0.04, 0.05, and 0.29, respectively; and those of PSO with gamma-IFN at the concentrations of 10(2), 10(3), 10(4) and 10(5) U/ml were 1.00, 0.66, 0.30 and 0.28, respectively. The growth inhibition (%) of the HSC3 cells transplanted tumor in nude mice by gamma-IFN, PYM, gamma-IFN plus PYM, PSO, and gamma-IFN plus PSO were 67.6, 103.5, 110.0, 83.1 and 102.9, respectively; the body weight loss (%) of the treated mice were 1.2, 6.9, 0.8, 5.0 and 1.8 respectively, and that of the control was 12.5. The results indicated that the combination effects of gamma-IFN with PYM or gamma-IFN with PSO are synergic and beneficial to the general health of the victim mice.
Wu
J
J
College of Stomatology, Fourth Military Medical University, Xi'an.
Situ
Z
Z
Wang
W
W
Chen
J
J
Liu
B
B
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology* / Q000031:analogs & derivatives; D004357:Drug Synergism; D005363:Ficusin / Q000494:pharmacology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014062:Tongue Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D001761:Bleomycin; C021771:zhengguangmycin; D007371:Interferon-gamma; D005363:Ficusin