Inhibition of plasma extravasation by abruquinone A, a natural isoflavanquinone isolated from Abrus precatorius.
Polymyxin B-induced hind-paw edema was suppressed by abruquinone A, an isoflavanquinone isolated from Abrus precatorius, in normal as well in adrenalectomized mice. Unlike dexamethasone, abruquinone A did not increase the liver glycogen content in fasting adrenalectomized mice. The volume of exuded plasma was significantly reduced by abruquinone A in neurogenic inflammation, passive cutaneous anaphylactic reaction and compound 48/80-induced ear edema. Histamine-, serotonin-, bradykinin- and substance P-induced plasma extravasation in ear edema was also suppressed by abruquinone A. Abruquinone A, like isoproterenol, significantly reduced the bradykinin- and substance P-induced plasma extravasation in normal as well as in compound 48/80-pretreated mice. In addition, abruquinone A suppressed the bradykinin- and substance P-induced ear edema to a significantly greater extent than diphenhydramine/methysergide did. In the in vitro experiments, abruquinone A suppressed the compound 48/80-induced histamine and beta-glucuronidase released from isolated rat peritoneal mast cell preparations. These results suggest that the anti-inflammatory effect of abruquinone A is mediated partly via the suppression of the release of chemical mediators from mast cells and partly via the prevention of vascular permeability changes caused by mediators. The glucocorticoid activity and the release of glucocorticoid hormones from the adrenal gland are probably not involved.
Wang
J P
JP
Department of Medical Research, Taichung Veterans General Hospital, Taiwan.
Hsu
M F
MF
Chang
L C
LC
Kuo
J S
JS
Kuo
S C
SC
D000315:Adrenalectomy; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal; D001578:Benzopyrans / Q000494:pharmacology*; D016227:Benzoquinones / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects*; D015550:Cell Degranulation / Q000187:drug effects; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D005938:Glucocorticoids / Q000096:biosynthesis; D005966:Glucuronidase / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects; D007249:Inflammation / Q000473:pathology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D051379:Mice; D008813:Mice, Inbred ICR; D010323:Passive Cutaneous Anaphylaxis / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001578:Benzopyrans; D016227:Benzoquinones; D005938:Glucocorticoids; D003189:p-Methoxy-N-methylphenethylamine; C093391:abruquinone A; D005966:Glucuronidase