Renin mRNA is synthesized locally in rat ocular tissues.
Components of the Renin Angiotensin System (RAS) have been detected in ocular tissues and fluids. The source of the ocular RAS proteins is unknown but possibilities include diffusion or leakage from the systemic circulation, specific uptake from the blood, or local synthesis. We have used RT-PCR and in situ hybridization (ISH) to show that renin mRNA is present in ocular tissues from 3 strains of rats. By RT-PCR, we found 10 of 15 ciliary body samples, 13 of 16 iris samples, and 1 of 3 retina samples were positive for renin mRNA. Also, 6 of 6 brain and 7 of 8 kidney samples were positive. Using ISH, we found renin mRNA in the ciliary muscle adjacent to the sclera extending into the choroid. Tissue near the outflow channels of the anterior chamber angle also labeled. Retinal labeling was weak but present in the nerve fiber layer. Clusters of grains, possibly representing blood vessels, were also seen in the ciliary body, iris, and retina using ISH. These results suggest the presence of a local ocular RAS.
Brandt
C R
CR
Department of Ophthalmology and Visual Sciences, University of Wisconsin Medical School, Madison 53706-1532.
Pumfery
A M
AM
Micales
B
B
Bindley
C D
CD
Lyons
G E
GE
Sramek
S J
SJ
Wallow
I H
IH
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D017931:DNA Primers; D005123:Eye / Q000378:metabolism*; D017403:In Situ Hybridization; D007668:Kidney / Q000378:metabolism; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley; D012083:Renin / Q000096:biosynthesis* / Q000235:genetics
D017931:DNA Primers; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D012083:Renin