A potassium channel beta subunit related to the aldo-keto reductase superfamily is encoded by the Drosophila hyperkinetic locus.
Genetic and physiological studies of the Drosophila Hyperkinetic (Hk) mutant revealed defects in the function or regulation of K+ channels encoded by the Shaker (Sh) locus. The Hk polypeptide, determined from analysis of cDNA clones, is a homologue of mammalian K+ channel beta subunits (Kv beta). Coexpression of Hk with Sh in Xenopus oocytes increases current amplitudes and changes the voltage dependence and kinetics of activation and inactivation, consistent with predicted functions of Hk in vivo. Sequence alignments show that Hk, together with mammalian Kv beta, represents an additional branch of the aldo-keto reductase superfamily. These results are relevant to understanding the function and evolutionary origin of Kv beta.
Chouinard
S W
SW
Laboratory of Genetics, University of Wisconsin, Madison 53706, USA.
Wilson
G F
GF
Schlimgen
A K
AK
Ganetzky
B
B
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000235:genetics; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D004594:Electrophysiology; D015723:Gene Library; D017344:Genes, Insect / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009865:Oocytes; D010088:Oxidoreductases / Q000235:genetics; D015221:Potassium Channels / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D012313:RNA; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D051637:Shaker Superfamily of Potassium Channels; D014981:Xenopus
D018076:DNA, Complementary; D029721:Drosophila Proteins; C094813:Hk (hyperkinetic) protein, Drosophila; D015221:Potassium Channels; C039919:RNA, recombinant; D011994:Recombinant Proteins; C496929:Sh protein, Drosophila; D051637:Shaker Superfamily of Potassium Channels; D012313:RNA; D010088:Oxidoreductases