title: Members of the 70 kDa heat shock protein family specifically recognize sulfoglycolipids: role in gamete recognition and mycoplasma-related infertility.
abstract: We have previously shown that several mycoplasma species associated with infertility bind specifically to sulfated glycolipids isolated from the mammalian reproductive tract. We now show that a germ cell-specific sulfoglycolipid binding protein (SLIP 1), which is a potent inhibitor of sperm/egg binding in vitro, is immunologically related to the heat shock protein(Hsp) 70 family of stress proteins and that Hsps are surface antigens in male germ cells. Our present data demonstrate that several mycoplasma and mammalian Hsps share this glycolipid binding specificity in vitro, and suggest that surface Hsps can function as adhesins which mediate sulfoglycolipid recognition in infectious disease and normal reproductive physiology.
authors.affiliation: Department of Microbiology, Hospital for Sick Children, Toronto, Ontario, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Boulanger
authors.forename: D
authors.initials: D
authors.lastname: Faulds
authors.forename: E M
authors.initials: EM
authors.lastname: Eddy
authors.forename: C A
authors.initials: CA
authors.lastname: Lingwood
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion; D018797:Cell Cycle Proteins; D006017:Glycolipids / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D066298:In Vitro Techniques; D007246:Infertility / Q000378:metabolism; D008297:Male; D009174:Mycoplasma / Q000737:chemistry; D009175:Mycoplasma Infections / Q000150:complications; D051381:Rats; D013094:Spermatozoa / Q000166:cytology / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D006017:Glycolipids; D018840:HSP70 Heat-Shock Proteins; C051337:Slip1 protein, rat; C011117:sulfoglycolipids