Biological and molecular studies on Syrian hamster intracisternal R-type particles.
Retrovirus-like intracisternal R-type particles (IRP) are structures present in Syrian hamster (Mesocricetus auratus) cells cultured in vitro where they appear either spontaneously or under chemical induction conditions. We have tested several chemical inducers and ten different cell lines, looking for the best IRP induction conditions. BHK21 cl. 13 showed the highest inducibility one day after a 24 h treatment with 1 microgram/ml of 5-aza-2'-deoxycytidine. Using detergent treatments and sucrose gradients, we obtained semi-purified IRP cores. A 7.2 kb RNA associated with the core fraction was revealed by hybridization with total Syrian hamster genomic DNA, but not with Syrian hamster intracisternal A particle (IAP) specific probes. This suggests that the IRP genes are distinct from IAP ones.
Lesser
J
J
UPR A0043, CNRS Rétrovirus et Rétrotransposons des Vertébrés, Hôpital Saint-Louis, Paris, France.
Bittoun
P
P
Emanoil-Ravier
R
R
Peries
J
J
D000818:Animals; D001374:Azacitidine / Q000031:analogs & derivatives / Q000494:pharmacology; D002460:Cell Line; D006224:Cricetinae; D000077209:Decitabine; D005786:Gene Expression Regulation / Q000187:drug effects; D008647:Mesocricetus / Q000821:virology*; D008854:Microscopy, Electron; D015335:Molecular Probes; D012367:RNA, Viral / Q000235:genetics; D012190:Retroviridae / Q000235:genetics* / Q000302:isolation & purification
D015335:Molecular Probes; D012367:RNA, Viral; D000077209:Decitabine; D001374:Azacitidine