title: Structures of DNA and RNA polymerases and their interactions with nucleic acid substrates.
abstract: DNA and RNA polymerases are enzymes that are primarily responsible for copying genetic material in all living systems. The four polymerases whose structures have been determined by X-ray crystallographic methods have significant similarities at the polymerase active site that are indicative of common requirements for polynucleotide synthesis. Structural studies of complexes of the Klenow fragment of Escherichia coli DNA polymerase I, HIV type 1 reverse transcriptase, and rat DNA polymerase beta with DNA are leading to generalized models for catalysis.
authors.affiliation: Center for Advanced Biotechnology and Medicine and Rutgers University Chemistry Department, Piscataway, NJ 08854-5638, USA.
authors.forename: E
authors.initials: E
authors.lastname: Arnold
authors.forename: J
authors.initials: J
authors.lastname: Ding
authors.forename: S H
authors.initials: SH
authors.lastname: Hughes
authors.forename: Z
authors.initials: Z
authors.lastname: Hostomsky
mesh_terms: D000818:Animals; D004256:DNA Polymerase I / Q000737:chemistry / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D009696:Nucleic Acids / Q000737:chemistry* / Q000378:metabolism*; D011487:Protein Conformation; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism; D051381:Rats
chemical_list: D009696:Nucleic Acids; D012194:RNA-Directed DNA Polymerase; D012321:DNA-Directed RNA Polymerases; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase