The granulocyte colony-stimulating factor produced in the human lung and its effect on liquid movement in the rabbit lung.
Levels of the granulocyte colony-stimulating factor (G-CSF) were determined in the plasma and resected lung tissue from patients who underwent pulmonary resection. Moreover, the effect of recombinant human (rh) G-CSF on the permeability of pulmonary endothelium and on liquid clearance from the alveolar spaces was investigated in rabbits. The plasma levels of G-CSF increased from 30 pg/ml preoperatively to 409 +/- 236 pg/ml 3 h postoperatively (P < 0.05), while the levels of G-CSF in the resected lung tissue were increased in the alveolar fluid, to 1,834 +/- 1,054 pg/ml, and in the pulmonary blood, to 5,466 +/- 2,019 pg/ml. It was found that rh G-CSF 25 micrograms administered into the subcutaneous tissue of rabbits increased extravascular lung water to 3.45 +/- 0.26 vs 2.98 +/- 0.20 in control experiments (P < 0.05); however, rhG-CSF 0.75 microgram/kg administered into the alveolar spaces did not affect liquid clearance from the alveolar spaces. The findings of this study led us to conclude that G-CSF is synthesized in the human lung and increases the permeability of pulmonary endothelium, but not liquid clearance across the alveolar epithelium.
Sakuma
T
T
Department of Surgery, Sendai Kosei Hospital, Japan.
Nakada
T
T
Nishimura
T
T
Hoshikawa
Y
Y
Fujimura
S
S
D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D003627:Data Interpretation, Statistical; D004727:Endothelium / Q000378:metabolism; D015633:Extravascular Lung Water / Q000378:metabolism / Q000502:physiology*; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis* / Q000097:blood / Q000502:physiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007958:Leukocyte Count; D008168:Lung / Q000378:metabolism*; D009504:Neutrophils; D010539:Permeability; D011013:Pneumonectomy; D011184:Postoperative Period; D011650:Pulmonary Alveoli / Q000378:metabolism / Q000502:physiology; D011817:Rabbits
D016179:Granulocyte Colony-Stimulating Factor