Autoimmunity in Chagas disease cardiopathy: biological relevance of a cardiac myosin-specific epitope crossreactive to an immunodominant Trypanosoma cruzi antigen.
Heart tissue destruction in chronic Chagas disease cardiopathy (CCC) may be caused by autoimmune recognition of heart tissue by a mononuclear cell infiltrate decades after Trypanosoma cruzi infection. Indirect evidence suggests that there is antigenic crossreactivity between T. cruzi and heart tissue. As there is evidence for immune recognition of cardiac myosin in CCC, we searched for a putative myosin-crossreactive T. cruzi antigen. T. cruzi lysate immunoblots were probed with anti-cardiac myosin heavy chain IgG antibodies (AMA) affinity-purified from CCC or asymptomatic Chagas disease patient-seropositive sera. A 140/116-kDa doublet was predominantly recognized by AMA from CCC sera. Further, recombinant T. cruzi protein B13--whose native protein is also a 140- and 116-kDa double band--was identified by crossreactive AMA. Among 28 sera tested in a dot-blot assay, AMA from 100% of CCC sera but only 14% of the asymptomatic Chagas disease sera recognized B13 protein (P = 2.3 x 10(-6)). Sequence homology to B13 protein was found at positions 8-13 and 1442-1447 of human cardiac myosin heavy chain. Competitive ELISA assays that used the correspondent myosin synthetic peptides to inhibit serum antibody binding to B13 protein identified the heart-specific AAALDK (1442-1447) sequence of human cardiac myosin heavy chain and the homologous AAAGDK B13 sequence as the respective crossreactive epitopes. The recognition of a heart-specific T. cruzi crossreactive epitope, in strong association with the presence of chronic heart lesions, suggests the involvement of crossreactivity between cardiac myosin and B13 in the pathogenesis of CCC.
Cunha-Neto
E
E
Laboratory of Transplantation Immunology, São Paulo University Hospital, Faculty of Medicine, Brazil.
Duranti
M
M
Gruber
A
A
Zingales
B
B
De Messias
I
I
Stolf
N
N
Bellotti
G
G
Patarroyo
M E
ME
Pilleggi
F
F
Kalil
J
J
D000595:Amino Acid Sequence; D000953:Antigens, Protozoan / Q000097:blood / Q000276:immunology*; D015551:Autoimmunity; D001667:Binding, Competitive; D002598:Chagas Cardiomyopathy / Q000209:etiology / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D000939:Epitopes / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D016056:Immunodominant Epitopes / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood; D008969:Molecular Sequence Data; D009206:Myocardium / Q000276:immunology* / Q000473:pathology; D009218:Myosins / Q000235:genetics / Q000276:immunology*
D000953:Antigens, Protozoan; D000939:Epitopes; D016056:Immunodominant Epitopes; D007074:Immunoglobulin G; D009218:Myosins