Induction of cellular follicle-stimulating hormone in the hamster adenohypophysis requires intermittent stimulation by luteinizing hormone releasing hormone.
We investigated the effectiveness of continuous vs intermittent LHRH stimulation of the neonatal female anterior pituitary gland on inducing cellular FSH immunoreactivity in the Golden Syrian hamster. Neonatal female pituitary glands were grafted beneath the right renal capsules of hypophysectomized-ovariectomized adult hosts with a catheter implanted in the external jugular vein. In experiment 1, vehicle or LHRH (6 ng/h) was infused continuously or LHRH was pulsed at 1 h (6 ng) or 12 h (72 ng) intervals through the catheters for 8 days. Hamsters were decapitated for collection of trunk blood shortly after the end of treatment, and grafts were prepared for immunocytochemical staining for LH and FSH. Anterior pituitary glands removed from neonatal (day 1) and day 9 female pups also were stained for LH and FSH. The mean percentage of adenohypophysial cells staining for LH increased from 11% in neonatal pups to mean percentages (24-28%) that were similar in day 9 pups and in all groups with grafts. The mean percentage of adenohypophysial cells staining for FSH increased from 1% in neonatal pups to percentages (16-21%) that were similar in day 9 pups and in grafts in hosts administered 6 or 72 ng LHRH pulses. By contrast, the mean percentage of FSH cells did not increase in grafts in hosts administered vehicle or LHRH by continuous infusion. Serum LH concentration was low in hosts given vehicle or LHRH by continuous infusion but elevated in hosts given 72 ng LHRH pulses and in all but one host given 6 ng LHRH pulses.(ABSTRACT TRUNCATED AT 250 WORDS)
Woller
M J
MJ
Department of Cell Biology and Neuroscience, University of South Carolina School of Medicine, Columbia 29208, USA.
Campbell
G T
GT
Blake
C A
CA
D000818:Animals; D000831:Animals, Newborn; D006224:Cricetinae; D005260:Female; D005640:Follicle Stimulating Hormone / Q000032:analysis / Q000096:biosynthesis*; D007987:Gonadotropin-Releasing Hormone / Q000008:administration & dosage* / Q000494:pharmacology; D007016:Hypophysectomy; D007150:Immunohistochemistry; D007668:Kidney; D007986:Luteinizing Hormone / Q000032:analysis; D008647:Mesocricetus; D010052:Ovariectomy; D010902:Pituitary Gland / Q000637:transplantation; D010903:Pituitary Gland, Anterior / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D016042:Transplantation, Heterotopic
D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone