Mechanism of allergic cross-reactions. V. High incidence of unanticipated cross-stimulation by natural allergens of rat basophilic leukemia cells sensitized with monoclonal IgE antibodies.
The incidence of cross-stimulations by natural allergens was investigated using RBL-2H3 cells sensitized with five different mouse monoclonal anti-DNP IgEs and four mercury-induced rat monoclonal IgEs. Cells sensitized with 3 of the 5 monoclonal anti-DNP IgEs (clones SPE-7, SRT-1, LB4) responded by serotonin release upon stimulation by natural allergens such as Dermatophagoides pteronyssinus, horse dander and mugwort extracts. Serotonin release could be inhibited by monovalent DNP-lysine, indicating the involvement of DNP-binding sites of IgEs. Two of the clones (LO-DNP-30 and LA2) were negative on all tests with allergens. All but one (Hg32) of the mercury-induced rat IgE monoclonal antibodies tested positive with DNP-BSA, and with at least one of the six allergen extracts. IgE clone Hg12 mediated serotonin release with 5 of the 6 allergens tested.
Varga
J M
JM
Department of Dermatology, University of Innsbruck, Austria.
Kalchschmid
G
G
Bellon
B
B
Kuhn
J
J
Druet
P
P
Fritsch
P
P
D000485:Allergens / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D039741:Antigens, Dermatophagoides; D015550:Cell Degranulation; D003429:Cross Reactions; D006023:Glycoproteins / Q000276:immunology; D007073:Immunoglobulin E / Q000276:immunology*; D015471:Leukemia, Basophilic, Acute; D008407:Mast Cells / Q000276:immunology*; D008925:Mites / Q000276:immunology; D051381:Rats; D012701:Serotonin / Q000276:immunology
D000485:Allergens; D000911:Antibodies, Monoclonal; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D012701:Serotonin; D007073:Immunoglobulin E