title: The dynamics of beta 1 integrin expression during peripheral nerve regeneration.
abstract: The aim of the present study was to examine the expression of beta 1 integrin subunit after peripheral nerve transection. After sciatic nerve transection two experimental procedures were used; changes in the freely regenerating rat sciatic nerve were compared to a situation in which spontaneous regeneration was prevented by suturing both ends of the nerve to the muscle next to the point of transection. Specimens for morphological analysis were collected 6 h, 1, 3, 5, 7 days and 2, 4, 6 and 8 weeks after the axotomy. Sections from the proximal (two zones) and distal (three zones) stumps next to the point of transection were stained with antibodies against beta 1 integrin subunit, macrophages, collagen types I and III, and S-100 protein. The control nerves showed beta 1 integrin-stained cells in the perineurium and vasa nervorum but the endoneurium was negative. Positively stained endoneurial fibroblast-like cells could be seen in the proximal part of the nerve already at 24 h after transection. The number of these positively stained cells increased steadily; they were most numerous 4 weeks after transection in the distal zone 2. Subsequently, the number of positively stained endoneural cells declined sharply and 8 weeks after transection no positively stained cells could be found. The morphological appearance and the immunohistochemical properties of the cells suggest that the majority of beta 1 integrin-positive cells are endoneurial fibroblast-like cells. Thus, the process appeared to be dynamic, starting from the proximal part and continuing to the distal parts, and was similar in both experimental groups.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pathology, University of Turku, Finland.
authors.forename: H S
authors.initials: HS
authors.lastname: Taskinen
authors.forename: J
authors.initials: J
authors.lastname: Heino
authors.forename: M
authors.initials: M
authors.lastname: Röyttä
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D003094:Collagen / Q000502:physiology; D019012:Integrin beta1; D016023:Integrins / Q000378:metabolism / Q000502:physiology*; D008297:Male; D009416:Nerve Regeneration / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012584:Sciatic Nerve / Q000166:cytology / Q000293:injuries / Q000502:physiology*
chemical_list: D019012:Integrin beta1; D016023:Integrins; D003094:Collagen