12-lipoxygenase mRNA expression by cultured neurons.
12-hydroxy 5,8,14-cis 10-trans eicosatetraenoic acid (12-HETE) and its derivatives are the principal lipoxygenase (Lox) products of the mammalian brain. These metabolites have been proposed to play a key role as second messengers in synaptic transmission and might function as retrograde messengers in learning and memory processes: the long-term potentiation. The exact source(s) of 12-HETE and neuronal implication have not been definitively established. The present work was therefore designed to study 12-Lox mRNA expression in neural cell cultures. Detection of this mRNA from cellular extract was obtained by reverse transcriptase-polymerase chain reaction (RT-PCR) and localization in neurons by in situ RT-PCR. These results argue for 12-Lox neuronal production.
Palluy
O
O
URA CNRS 1485, Faculté de Médecine et de Pharmacie de Limoges, France.
Bendani
M
M
Vallat
J M
JM
Rigaud
M
M
D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000235:genetics*; D002478:Cells, Cultured; D009474:Neurons / Q000166:cytology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase; D051381:Rats
D012333:RNA, Messenger; D001092:Arachidonate 12-Lipoxygenase; D012194:RNA-Directed DNA Polymerase