Use of UV ParaLens adapter for detection of acid-fast organisms.
Auramine-stained mycobacterial smears from 136 clinical specimens were interpreted by using the UV ParaLens adapter (Beckton Dickinson), and results were compared with smear interpretations using a traditional fluorescent microscope and culture. The sensitivity and specificity of the ParaLens were 84 and 93%, respectively. Smears yielding discrepant results were overstained by the Kinyoun method. Overall, the sensitivity of auramine-stained smears interpreted with the UV ParaLens was comparable to that of Kinyoun-stained smears.
Patterson
K V
KV
Department of Pathology, University of South Alabama, Mobile 36617.
McDonald
C L
CL
Miller
B F
BF
Chapin
K C
KC
D001576:Benzophenoneidum; D001826:Body Fluids / Q000382:microbiology; D005456:Fluorescent Dyes; D006801:Humans; D008860:Microscopy, Ultraviolet / Q000295:instrumentation*; D009161:Mycobacterium / Q000166:cytology / Q000254:growth & development; D009164:Mycobacterium Infections / Q000175:diagnosis*; D013183:Sputum / Q000382:microbiology; D013194:Staining and Labeling
D005456:Fluorescent Dyes; D001576:Benzophenoneidum