A specific immunoradiometric assay of cationic trypsin(ogen) that does not recognize trypsin-alpha-1-proteinase inhibitor complex.
An immunoradiometric assay using two monoclonal antibodies directed to human trypsin 1 was developed for measuring trypsin(ogen) in biological fluids. The assay is different from other assays in that it is specific for cationic trypsinogen and does not recognize the alpha-1-proteinase inhibitor-trypsin complex. It can be used as a complement to classical immunoassays to characterize trypsinogen activation in pathological cases. The evaluation and the specificity of the assay are presented.
Lafont
P
P
Centre hospitalier Lyon-Sud, Pierre-Bénite, France.
Guy-Crotte
O
O
Paulin
C
C
Galvain
D
D
Mertani
S
S
Figarella
C
C
Barbier
Y
Y
D000510:Alpha-Globulins / Q000032:analysis*; D000653:Amniotic Fluid / Q000201:enzymology; D000818:Animals; D006801:Humans; D015592:Immunoradiometric Assay / Q000379:methods*; D010189:Pancreatic Juice / Q000201:enzymology; D011863:Radioimmunoassay; D051381:Rats; D013552:Swine; D014357:Trypsin / Q000032:analysis* / Q000097:blood; D014361:Trypsin Inhibitors / Q000032:analysis* / Q000097:blood; D014362:Trypsinogen / Q000032:analysis* / Q000097:blood
D000510:Alpha-Globulins; D014361:Trypsin Inhibitors; C011877:inter-alpha-inhibitor; D014362:Trypsinogen; D014357:Trypsin