Cell swelling activates separate taurine and chloride channels in Ehrlich mouse ascites tumor cells.
The taurine efflux from Ehrlich ascites tumor cells is stimulated by hypotonic cell swelling. The swelling-activated taurine efflux is unaffected by substitution of gluconate for extracellular Cl- but inhibited by addition of MK196 (anion channel blocker) and 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS; anion channel and anion exchange blocker) and by depolarization of the cell membrane. This is taken to indicate that taurine does not leave the osmotically swollen Ehrlich cells in exchange for extracellular Cl-, i.e., via the anion exchanger but via a MK196- and DIDS-sensitive channel that is potential dependent. An additional stimulation of the swelling-activated taurine efflux is seen after addition of arachidonic acid and oleic acid. Cell swelling also activates a "Mini Cl- channel." The Cl- efflux via this Cl- channel, in contrast to the swelling-activated taurine efflux, is unaffected by DIDS and inhibited by arachidonic acid and oleic acid. It is suggested that the swelling-activated "Mini Cl- channel" and the swelling-activated taurine channel in the Ehrlich cell represent two distinct types of channels.
Lambert
I H
IH
August Krogh Institute, Biochemical Department, University of Copenhagen, Denmark.
Hoffmann
E K
EK
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D002286:Carcinoma, Ehrlich Tumor; D048429:Cell Size; D018118:Chloride Channels / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism*; D006096:Gramicidin / Q000494:pharmacology; D007189:Indans / Q000494:pharmacology; D007473:Ion Channels / Q000378:metabolism*; D007700:Kinetics; D008564:Membrane Potentials; D051379:Mice; D009994:Osmolar Concentration; D013654:Taurine / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D018118:Chloride Channels; D002712:Chlorides; D007189:Indans; D007473:Ion Channels; D006096:Gramicidin; D013654:Taurine; D016718:Arachidonic Acid; C013352:indacrinone; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid