Presence of the intermediate filaments cytokeratins and vimentin in the rat corpus luteum during luteal life-span.
The presence of the intermediate filament (IF) proteins cytokeratins and vimentin in corpus luteum (CL) and other parts of the ovary from adult pseudopregnant rats was investigated using immunohistochemistry and immunoblot analysis. To induce pseudopregnancy, female rats were mated with sterile male rats. The mating procedure induces a prolonged luteal life-span of 13 +/- 1 days. Positive staining for cytokeratin could be seen in CL, in the theca layer of follicles, and the ovarian surface epithelium with the broad-spectrum monoclonal antibody cocktail AE1/AE3. Weak staining was also seen in CL with antibodies against cytokeratins 8 and 18. A similar distribution was also seen for vimentin, which furthermore was detected in blood vessels. No changes in staining intensity was seen in CL of different luteal age. The strong staining for vimentin in CL was confirmed by immunoblot analysis, where one main band of 57 kDa was observed from day 1 to day 19 of pseudopregnancy. Expression of the IF proteins investigated seems to start in the newly formed CL and the continuous expression indicates that they are not directly regulated by luteal steroids.
Nilsson
I
I
Department of Physiology, University of Umeå, Sweden.
Mattsson
M O
MO
Selstam
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D003338:Corpus Luteum / Q000737:chemistry* / Q000502:physiology*; D005260:Female; D015151:Immunoblotting; D007633:Keratins / Q000032:analysis*; D008297:Male; D010053:Ovary / Q000737:chemistry / Q000502:physiology; D011374:Progesterone / Q000097:blood; D011555:Pseudopregnancy / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling / Q000379:methods; D014746:Vimentin / Q000032:analysis*
D000911:Antibodies, Monoclonal; D014746:Vimentin; D011374:Progesterone; D007633:Keratins