Lectin staining of sheep microglia.
The B4-isolectin from Griffonia simplicifolia is known to stain microglial cells in a variety of species. The present report describes a lectin staining method that has been modified to facilitate staining of resting microglia, as well as perivascular cells, in vibratome sections of normal sheep brain. This modified method employs tissue fixed in formaldehyde or paraformaldehyde and requires incubating sections with Triton X-100 prior to staining.
Pennell
N A
NA
Department of Neuroscience, JHMHC, University of Florida, Gainesville 32610.
Hurley
S D
SD
Streit
W J
WJ
D000818:Animals; D005404:Fixatives; D037102:Lectins; D008867:Microtomy; D009457:Neuroglia / Q000166:cytology*; D012756:Sheep / Q000033:anatomy & histology*; D013194:Staining and Labeling / Q000379:methods
D005404:Fixatives; D037102:Lectins