Preparation of sterically stabilized human serum albumin nanospheres using a novel Dextranox-MPEG crosslinking agent.
Human serum albumin (HSA) nanospheres with a size less than 200 nm in diameter were prepared using a modified coacervation method and crosslinking with methyl polyethylene glycol modified oxidized Dextram (Dextranox-MPEG) which created a sterically stabilizing polyethylene oxide surface layer surrounding the nanospheres. The crosslinking efficiency and the surface characteristics of glutaraldehyde and Dextranox-MPEG crosslinked HSA nanospheres were determined and compared. The zeta potential of the Dextranox-MPEG crosslinked particles was significantly lower than that of glutaraldehyde stabilized particles. The existence of a hydrated steric barrier surrounding the nanospheres was confirmed by an electrolyte and pH induced flocculation test. The Dextranox-MPEG crosslinked nanospheres showed a significantly reduced plasma protein adsorption on the particle surface compared with glutaraldehyde crosslinked nanospheres.
Lin
W
W
Department of Pharmaceutical Sciences, University of Nottingham, University Park, UK.
Coombes
A G
AG
Garnett
M C
MC
Davies
M C
MC
Schacht
E
E
Davis
S S
SS
Illum
L
L
D002626:Chemistry, Pharmaceutical; D003432:Cross-Linking Reagents; D003911:Dextrans; D006801:Humans; D008863:Microspheres; D010316:Particle Size; D011092:Polyethylene Glycols; D011487:Protein Conformation; D012709:Serum Albumin / Q000737:chemistry*; D013499:Surface Properties
D003432:Cross-Linking Reagents; D003911:Dextrans; D012709:Serum Albumin; C092277:dextranox-methyl polyethylene glycol; D011092:Polyethylene Glycols