title: Plasmodium falciparum: parasites defective in early stages of gametocytogenesis.
abstract: Some molecular characteristics of Plasmodium falciparum lines which do not produce gametocytes are described. Parasites carrying a subtelomerically deleted chromosome 9 cannot form even the earliest forms of gametocytes, detectable with antibodies against the gametocyte-specific antigen Pfg27. In a parasite culture of clone HB3, in which both intact and deleted forms of chromosome 9 are present, full-length chromosome 9 molecules are retained mainly in gametocytes. These data suggest that the subtelomeric portion of chromosome 9 is required at an early stage of gametocytogenesis. Parasite subclones derived from gametocyte producing clone 3D7, which completely lost ability to produce gametocytes, are also described. Unlike the previous gametocyteless lines, these parasites stably maintain a full-length chromosome 9 and the ability to cytoadhere to C32 melanoma cells after prolonged asexual propagation. Their defect in sexual development is therefore genetically and functionally distinct from that of parasites carrying a deleted chromosome 9. Gametocyteless subclones derived from 3D7 do not produce any Pfg27 mRNA, while this gene is anomalously expressed in asexual stage parasites of two lines of a different genetic background, 1776sel8 and C10, one able and the other unable to produce gametocytes.
authors.affiliation: Laboratorio di Biologia Cellulare, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Alano
authors.forename: L
authors.initials: L
authors.lastname: Roca
authors.forename: D
authors.initials: D
authors.lastname: Smith
authors.forename: D
authors.initials: D
authors.lastname: Read
authors.forename: R
authors.initials: R
authors.lastname: Carter
authors.forename: K
authors.initials: K
authors.lastname: Day
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002869:Chromosome Aberrations; D002872:Chromosome Deletion; D005455:Fluorescent Antibody Technique; D005718:Gametogenesis; D010641:Phenotype; D010963:Plasmodium falciparum / Q000235:genetics* / Q000254:growth & development*; D017384:Sequence Deletion; D016615:Telomere