title: Isolation and ex vivo expansion of CD34+ cells from cord blood using dextran sedimentation and avidin column selection.
abstract: Human umbilical cord bloods were fractionated by unit gravity sedimentation in 1% (v/v) dextran, followed by immunoaffinity selection for CD34+ stem and progenitor cells. Dextran sedimentation alone enabled recovery of more than 80% of the nucleated cells present and 90% of the CD34+ cells, as determined by flow cytometry. The addition of an immunoaffinity selection step for CD34+ cells resulted in a 134-fold enrichment for CD34+ cells, with a mean yield of 64 +/- 15%. The resultant CD34+ population contained almost half the CFU-GM activity initially present in the cord bloods and could be expanded ex vivo in liquid culture.
authors.affiliation: CellPro, Inc., Bothell, WA 98021.
authors.forename: S
authors.initials: S
authors.lastname: Heimfeld
authors.forename: D F
authors.initials: DF
authors.lastname: Kalamasz
authors.forename: B L
authors.initials: BL
authors.lastname: Fogarty
authors.forename: R
authors.initials: R
authors.lastname: Fei
authors.forename: Z N
authors.initials: ZN
authors.lastname: Tsui
authors.forename: H M
authors.initials: HM
authors.lastname: Jones
authors.forename: R J
authors.initials: RJ
authors.lastname: Berenson
mesh_terms: D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D001360:Avidin; D001710:Biotin; D001772:Blood Cell Count; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D002846:Chromatography, Affinity / Q000295:instrumentation; D003911:Dextrans; D005312:Fetal Blood / Q000166:cytology*; D005427:Flocculation; D006412:Hematopoietic Stem Cells; D006801:Humans; D007231:Infant, Newborn
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D003911:Dextrans; D001360:Avidin; D001710:Biotin