title: cAMP-activated chloride channels in a CFTR-transfected pancreatic adenocarcinoma-derived cell line, pANS6.
abstract: Pancreatic adenocarcinoma cell lines rarely express the CFTR gene, despite the high levels of CFTR protein that are present in primary pancreatic duct cells. We have attempted to generate a non-CF pancreatic adenocarcinoma cell line that stably produces high levels of CFTR mRNA and protein by transfecting a vector containing the CFTR cDNA, driven by a strong mammalian promoter, into the poorly differentiated pancreatic adenocarcinoma cell line, Panc-1. The pANS6 pancreatic duct cell line expresses substantial levels of CFTR mRNA, but little CFTR protein. Despite this we were able to detect low conductance chloride channels in 40% of patches, stimulated with cAMP, that have similar biophysical properties to CFTR.
authors.affiliation: Institute of Molecular Medicine, Oxford University, John Radcliffe Hospital, UK.
authors.forename: A N
authors.initials: AN
authors.lastname: Smith
authors.forename: C J
authors.initials: CJ
authors.lastname: Wardle
authors.forename: J P
authors.initials: JP
authors.lastname: Winpenny
authors.forename: B
authors.initials: B
authors.lastname: Verdon
authors.forename: M A
authors.initials: MA
authors.lastname: Gray
authors.forename: B E
authors.initials: BE
authors.lastname: Argent
authors.forename: A
authors.initials: A
authors.lastname: Harris
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics; D002460:Cell Line; D018118:Chloride Channels / Q000737:chemistry / Q000187:drug effects*; D000242:Cyclic AMP / Q000494:pharmacology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005822:Genetic Vectors; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D010190:Pancreatic Neoplasms / Q000235:genetics; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D018118:Chloride Channels; D008565:Membrane Proteins; D012333:RNA, Messenger; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D000242:Cyclic AMP