Organization and transcription of the mitochondrial ATP synthase genes in the yeast Yarrowia lipolytica.
Mitochondrial gene organization was studied in a dimorphic yeast, Yarrowia lipoltica. The gene order in a sequenced 6.6-kilobase region closely resembles that of the human mitochondrial genome in that ATP synthase subunit 8 and 6 genes are followed by genes for cytochrome c oxidase subunit 3 (which contains an intron), NADH-ubiquinone oxidoreductase subunit 4, and ATP synthase subunit 9. This region also contains tRNA genes decoding AUA, UGA, CUN and CCN codons, suggesting a unique mitochondrial translation. All the above genes are transcribed from the same DNA strand into multigenic RNAs, starting from a nonanucleotide sequence, 5'-ATATAAATA-3', similar to other yeast mitochondrial promoters.
Matsuoka
M
M
Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.
Matsubara
M
M
Inoue
J
J
Kakehi
M
M
Imanaka
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000302:isolation & purification / Q000378:metabolism; D005091:Exons; D005800:Genes, Fungal; D007438:Introns; D046911:Macromolecular Substances; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides; D006180:Proton-Translocating ATPases / Q000096:biosynthesis* / Q000235:genetics*; D012313:RNA / Q000096:biosynthesis / Q000302:isolation & purification; D000077278:RNA, Mitochondrial; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics; D015183:Restriction Mapping; D004718:Saccharomycetales / Q000201:enzymology* / Q000235:genetics*; D014158:Transcription, Genetic; D015003:Yeasts / Q000201:enzymology* / Q000235:genetics*
D003062:Codon; D017931:DNA Primers; D004272:DNA, Mitochondrial; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D000077278:RNA, Mitochondrial; D012313:RNA; D012343:RNA, Transfer; D006180:Proton-Translocating ATPases