title: Regulatory action of prolactin on the in vitro growth of CD34+ve human hemopoietic progenitor cells.
abstract: The pituitary hormone prolactin (Prl) is known to act as a local regulator of immune cell function, and Prl-binding receptors (Prl-R) have been described to share distinctive features with the members of the newly described cytokine/hemopoietin receptor superfamily. Here we show that the hormone can functionally interact with lineage-specific hemopoietic factors. When highly purified progenitor cells (CD34+ve) were seeded in semisolid methylcellulose cultures in the presence of interleukin (IL)-3, granulocyte-macrophage colony stimulating factor (GM-CSF), and erythropoietin (Epo), a selective enhancing effect of Prl on the formation of colony forming unit-granulocyte (CFU-G) and burst forming unit-erythroid (BFU-E) colonies was observed. The effect of the hormone was plotted as a bell shaped curve, with the optimal response at the supraphysiological concentration of 50 ng/ml. Limiting dilution analysis showed that Prl acted directly on hemopoietic progenitors. This was confirmed by the observation on the CD34+ve cells of Prl-binding sites reacting with the specific monoclonal antibodies (mAbs), U5 and PrR-7A. Immunoprecipitation of the metabolically labeled CD34+ve cells with the PrR-7A mAb revealed a structure of 43 kD under reducing conditions. Analysis of the early events associated with the Prl/Prl-R interaction showed an increased number of cells engaged in DNA and hemoglobin synthesis. Enhanced erythroid differentiation of CD34+ve cells in the presence of Prl was secondary to upmodulation of receptors for the lineage-specific factor Epo. Together these data demonstrate the existence of a functional interplay between Prl and hemopoietic factors.
authors.affiliation: Department of Clinical Physiopathology, University of Torino, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Bellone
authors.forename: M
authors.initials: M
authors.lastname: Geuna
authors.forename: A
authors.initials: A
authors.lastname: Carbone
authors.forename: S
authors.initials: S
authors.lastname: Silvestri
authors.forename: R
authors.initials: R
authors.lastname: Foa
authors.forename: G
authors.initials: G
authors.lastname: Emanuelli
authors.forename: L
authors.initials: L
authors.lastname: Matera
mesh_terms: D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000187:drug effects; D004921:Erythropoietin / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D011388:Prolactin / Q000276:immunology* / Q000494:pharmacology* / Q000502:physiology; D011981:Receptors, Prolactin / Q000276:immunology
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018952:Antigens, CD34; D003115:Colony-Stimulating Factors; D011981:Receptors, Prolactin; D004921:Erythropoietin; D011388:Prolactin