Tissue-specific, temporally regulated expression mediated by the proximal ovine beta-lactoglobulin promoter in transgenic mice.
The ovine beta-lactoglobulin gene is expressed abundantly in the mammary gland. This study determines whether the ovine beta-lactoglobulin promoter is sufficient for targeting tissue-specific expression in transgenic mice. To address this, the expression profile of an ovine beta-lactoglobulin promoter driven bacterial chloramphenicol acetyltransferase reporter construct was analysed. Comparison of the expression frequency of this hybrid transgene to that of a genomic beta-lactoglobulin transgene indicates that additional sequences, downstream of the promoter, are required for position-independent expression in transgenic mice. Nevertheless, the hybrid transgene was expressed specifically in the mammary gland. Furthermore, the hybrid transgene was expressed in the appropriate temporal pattern during pregnancy and lactation. Thus, the proximal promoter of the ovine beta-lactoglobulin gene contains sufficient sequence information to target expression to the mammary. This construct constitutes the basis for a compact mammary expression vector.
Webster
J
J
Division of Molecular Biology, Roslin Institute (Edinburgh), Scotland, UK.
Wallace
R M
RM
Clark
A J
AJ
Whitelaw
C B
CB
D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D007774:Lactation / Q000378:metabolism; D007782:Lactoglobulins / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008321:Mammary Glands, Animal / Q000254:growth & development / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D009928:Organ Specificity; D011247:Pregnancy; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D012756:Sheep / Q000235:genetics; D013997:Time Factors
D007782:Lactoglobulins; D011993:Recombinant Fusion Proteins; D015500:Chloramphenicol O-Acetyltransferase