Enzyme immunoassay of elcatonin in human plasma.
A sensitive and specific double-antibody enzyme immunoassay (EIA) for detecting an elcatonin-like immunoreactive substance (ECT-IS) in human plasma has been developed. In competitive reactions, the ECT antibody was incubated with a plasma sample (or ECT standard) and beta-D-galactosidase-linked synthetic ECT. Free and antibody-bound enzymes were separated using an anti-rabbit IgG-coated immunoplate. Enzyme activity on the plate was determined by fluorescence analysis. This immunoassay allows the detection of 20 to 300 fmol/ml (67 to 1000 pg/ml) of ECT. The EIA was applied to determine the pharmacokinetic behavior of ECT after a single intramuscular administration (20 IU). The maximum level was achieved 30 min after administration, at approximately 30 pg ECT/ml of plasma.
Takeyama
M
M
Department of Clinical Pharmacy, Oita Medical University, Japan.
Ikawa
K
K
Eto
I
I
Mori
K
K
D000328:Adult; D002116:Calcitonin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000201:enzymology; D005456:Fluorescent Dyes; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives; D007124:Immunoenzyme Techniques; D007273:Injections, Intramuscular; D008297:Male; D008875:Middle Aged; D001616:beta-Galactosidase / Q000276:immunology
D005456:Fluorescent Dyes; D006923:Hymecromone; C034888:4-methylumbelliferyl glucuronide; D002116:Calcitonin; D001616:beta-Galactosidase; C012755:elcatonin