title: Effects of interleukin-4 or stem cell factor on mast cell mediator release and cytokine gene expression.
abstract: We have investigated the capacity of interleukin (IL)-4 or stem cell factor (SCF) to induce direct mediator release from rodent peritoneal mast cells, and also to induce or regulate cytokine gene expression in the human HMC-1 mast cell line. SCF, but not IL-4, induced low levels of serotonin release from mouse or rat peritoneal mast cells; rat mast cells acquired enhanced responsiveness to SCF during culture. IL-4, but not SCF, enhanced ionomycin-induced transcription and secretion of several genes, including the cytokines IL-3, IL-4, granulocyte/macrophage-colony-stimulating factor, IL-8 and the receptor for IL-6 in the human HMC-1 mast cell line.
authors.affiliation: Department of Pharmacology and Therapeutics, University of Liverpool, UK.
authors.forename: J W
authors.initials: JW
authors.lastname: Coleman
authors.forename: M G
authors.initials: MG
authors.lastname: Buckley
authors.forename: A M
authors.initials: AM
authors.lastname: Taylor
authors.forename: E M
authors.initials: EM
authors.lastname: Banks
authors.forename: C M
authors.initials: CM
authors.lastname: Williams
authors.forename: M R
authors.initials: MR
authors.lastname: Holliday
authors.forename: J
authors.initials: J
authors.lastname: Thompson
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics; D015759:Ionomycin / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D018123:Receptors, Interleukin / Q000096:biosynthesis* / Q000235:genetics; D019947:Receptors, Interleukin-6; D012701:Serotonin / Q000378:metabolism; D019089:Stem Cell Factor
chemical_list: D016298:Hematopoietic Cell Growth Factors; D007378:Interleukins; D018123:Receptors, Interleukin; D019947:Receptors, Interleukin-6; D019089:Stem Cell Factor; D015847:Interleukin-4; D012701:Serotonin; D015759:Ionomycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor