title: Papillomavirus L1 capsids agglutinate mouse erythrocytes through a proteinaceous receptor.
abstract: Virus-like particles (VLPs) composed of L1 derived from bovine papillomavirus type 1 (BPV-1), several human papillomavirus types, or cottontail rabbit papillomavirus (CRPV) agglutinated mouse but not human or rat erythrocytes. Treatment of mouse erythrocytes with trypsin prevented hemagglutination (HA) by BPV-1. Sera from rabbits immunized with native CRPV VLPs, which protect against experimental CRPV infection, exhibited high titers of antibodies that inhibited CRPV VLP HA activity, while sera from rabbits immunized with denatured CRPV VLPs or native BPV VLPs, which do not protect against CRPV infection, were not inhibitory. Testing for HA inhibition is a rapid and simple method for examining the serological relatedness of papillomaviruses and measuring protective antibody titers after VLP vaccination.
authors.affiliation: Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland 20892, USA.
authors.forename: R B
authors.initials: RB
authors.lastname: Roden
authors.forename: N L
authors.initials: NL
authors.lastname: Hubbert
authors.forename: R
authors.initials: R
authors.lastname: Kirnbauer
authors.forename: F
authors.initials: F
authors.lastname: Breitburd
authors.forename: D R
authors.initials: DR
authors.lastname: Lowy
authors.forename: J T
authors.initials: JT
authors.lastname: Schiller
mesh_terms: D000818:Animals; D052688:Bovine papillomavirus 1 / Q000276:immunology / Q000502:physiology*; D002213:Capsid / Q000502:physiology*; D036022:Capsid Proteins; D002460:Cell Line; D012777:Cottontail rabbit papillomavirus / Q000276:immunology; D000939:Epitopes; D004912:Erythrocytes; D006384:Hemagglutination; D006801:Humans; D007106:Immune Sera; D051379:Mice; D009500:Neutralization Tests; D027383:Papillomaviridae / Q000276:immunology; D018411:Spodoptera
chemical_list: D036022:Capsid Proteins; D000939:Epitopes; D007106:Immune Sera; C085217:L1 protein, Bovine papillomavirus