Comparison of apo A-I-containing subpopulations of dog plasma and prenodal peripheral lymph: evidence for alteration in subpopulations in the interstitial space.
To study in vivo reverse cholesterol transport, dog plasma and lymph apo A-I-containing subpopulations were compared by two-dimensional electrophoresis. Charge and size of subpopulations were similar in plasma and lymph, but the distribution of subpopulations varied considerably. An increase in pre-beta and pre-alpha particles in lymph suggests these changes are a reflection of in vivo reverse cholesterol transport.
Asztalos
B F
BF
Department of Physiology, Louisiana State University Medical Center, New Orleans 70112, USA.
Sloop
C H
CH
Wong
L
L
Roheim
P S
PS
D000818:Animals; D016632:Apolipoprotein A-I / Q000032:analysis*; D001692:Biological Transport; D002784:Cholesterol / Q000378:metabolism; D004285:Dogs; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005110:Extracellular Space / Q000737:chemistry*; D008075:Lipoproteins, HDL / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D008196:Lymph / Q000737:chemistry*
D016632:Apolipoprotein A-I; D008075:Lipoproteins, HDL; D002784:Cholesterol