title: Investigation of enterohemorrhagic Escherichia coli O157:H7 adherence characteristics and invasion potential reveals a new attachment pattern shared by intestinal E. coli.
abstract: In this study, the interactions of enterohemorrhagic Escherichia coli (EHEC) O157 strains with human ileocecal (HCT-8) epithelial cells and HEp-2 cells were examined. EHEC adhered to, but did not invade, HCT-8 cells by the localized adherence mechanism and a heretofore unrecognized pattern which we called log jam. The log jam formation was (i) not observed on HEp-2 cells, (ii) independent of the EHEC eaeA gene required for localized adherence, and (iii) shared by pathogenic and nonpathogenic E. coli strains but not K-12 strains. The log jam phenotype may represent a basal means by which E. coli bacteria attach to the human intestine.
authors.affiliation: Department of Microbiology and Immunology, Uniformed Service University of the Health Sciences, F. Edward Hébert School of Medicine, Bethesda, Maryland 20814-4799, USA.
authors.forename: M L
authors.initials: ML
authors.lastname: McKee
authors.forename: A D
authors.initials: AD
authors.lastname: O'Brien
mesh_terms: D001422:Bacterial Adhesion / Q000276:immunology*; D002432:Cecum / Q000166:cytology; D002478:Cells, Cultured; D004926:Escherichia coli / Q000145:classification / Q000472:pathogenicity*; D006470:Hemorrhage; D006801:Humans; D007082:Ileum / Q000166:cytology; D007422:Intestines / Q000382:microbiology*; D007830:Larynx / Q000166:cytology; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D012703:Serotyping
chemical_list: D019081:O Antigens; D011135:Polysaccharides, Bacterial