Is endothelin-1 release at reperfusion of the ischaemic human heart due to cold-induced displacement of endothelin from binding sites?
Following reperfusion of ischaemic human hearts subjected to cold (4 degrees C) cardioplegia during coronary bypass surgery, there was an increase in cardiac outflow of endothelin-1 but not the pro-peptide big endothelin-1. Furthermore, specific endothelin-1 binding in human lung membrane preparations was displaced by incubation in buffer medium at 4 degrees C. The present results thus indicate that cold-induced displacement of endothelin-1 binding, rather than increased synthesis, may explain the cardiac release of endothelium-1 following ischaemia during heart surgery in which cold cardioplegia has been used.
Franco-Cereceda
A
A
Department of Thoracic Surgery, Karolinska Hospital, Stockholm, Sweden.
Barr
G
G
Owall
A
A
Liska
J
J
Lundberg
J M
JM
D000368:Aged; D003080:Cold Temperature; D001026:Coronary Artery Bypass; D019332:Endothelin-1; D016232:Endothelins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000378:metabolism*; D015425:Myocardial Reperfusion; D011498:Protein Precursors / Q000378:metabolism; D017466:Receptors, Endothelin / Q000378:metabolism*
D019332:Endothelin-1; D016232:Endothelins; D011498:Protein Precursors; D017466:Receptors, Endothelin