Functional coupling of the 5-HT2C serotonin receptor to G proteins in Xenopus oocytes.
The serotonin 2c (5-HT2C) receptor mediates its cellular effects by interacting with heterotrimeric guanine nucleotide binding proteins (G proteins). To characterize which G proteins are involved in functional coupling to the receptor, a mouse 5-HT2C receptor was expressed in Xenopus oocytes, and antisense oligoncleotides complementary to the mRNA sequence of the endogenous Xenopus G protein alpha subunits were used to inhibit G protein synthesis. Antisense oligonucleotide against the Xenopus G(o) alpha subunit inhibited the 5-HT2C receptor function, and coexpression of a rat G(o) alpha subunit reversed the inhibition by the anti-Xenopus G(o) oligonucleotide. Furthermore, antisense oligonucleotides against both the G(o) and Gi1 alpha subunits inhibited the electrophysiologic response induced by stimulation of the 5-HT2C receptor. These data suggest that both G(o) and Gi1 are involved in functional coupling of the 5-HT2C receptor to phospholipase C in Xenopus oocytes.
Chen
Y
Y
Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis 46202.
Baez
M
M
Yu
L
L
D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry / Q000502:physiology; D004594:Electrophysiology; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D009865:Oocytes / Q000201:enzymology / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D051381:Rats; D011985:Receptors, Serotonin / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism; D014981:Xenopus
D016376:Oligonucleotides, Antisense; D011985:Receptors, Serotonin; D012313:RNA; D010738:Type C Phospholipases; D019204:GTP-Binding Proteins