Comparative biochemical study of coated vesicles purified from cock brains.
Cock brain coated vesicles (CBCVs) were purified and compared with porcine brain coated vesicles (PBCVs) from several biochemical aspects. Clathrin heavy and light chains of CBCVs were immunologically similar to those of PBCVs. Coat proteins (CPs) of CBCVs behaved in almost the same manner as those of PBCVs for limited proteolysis. Dissociation of CPs from CBCVs by treatment with 10 mM Tris-Cl, pH 8.5, or 2M urea resembled that of CPs from PBCVs. pH dependency of dissociation of CPs from CBCVs was slightly different from those of PBCVs.
Yora
T
T
Department of Medical Technology, Saitama Medical School Junior College, Japan.
Sakagishi
Y
Y
Nakamura
H
H
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle / Q000276:immunology / Q000378:metabolism; D002645:Chickens / Q000276:immunology / Q000378:metabolism*; D002966:Clathrin / Q000032:analysis / Q000276:immunology; D003034:Coated Pits, Cell-Membrane / Q000737:chemistry*; D004705:Endocytosis; D000939:Epitopes / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology; D010196:Pancreatic Elastase / Q000378:metabolism; D010449:Peptide Mapping; D011817:Rabbits; D013045:Species Specificity; D013552:Swine / Q000276:immunology / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
D002966:Clathrin; D000939:Epitopes; D007106:Immune Sera; D008565:Membrane Proteins; D010196:Pancreatic Elastase; D014357:Trypsin