Diversity and genetic differentiation among subpopulations of Gliricidia sepium revealed by PCR-based assays.
Randomly amplified polymorphic DNA (RAPD), and a mitochondrial marker based on amplification of the V7 region of the mitochondrial small ribosomal RNA (srRNA) gene, were used to partition genetic variation within a single population of Gliricidia sepium sampled from Guatemala. Seventeen per cent of the variation detected with RAPDs was partitioned among subpopulations and indicated a greater level of discrimination than previously detected with isozymes. Cluster analysis indicated a direct relationship between this variation and the geographical distance between subpopulations. A polymorphism identified within the maternally inherited mitochondrial V7 srRNA product, which relied on digestion with restriction endonucleases, confirmed the genetic subdivision identified with RAPDs, and suggested a relatively limited role for seed in gene dispersal.
Dawson
I K
IK
Scottish Crop Research Institute, Invergowrie, Dundee, U.K.
Simons
A J
AJ
Waugh
R
R
Powell
W
W
D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004272:DNA, Mitochondrial / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D006166:Guatemala; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D012313:RNA / Q000235:genetics; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000235:genetics; D014197:Trees / Q000235:genetics*
D017931:DNA Primers; D004272:DNA, Mitochondrial; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; D012313:RNA