Potential interaction between annexin VI and a 56-kDa protein kinase in T cells.
Annexins belong to a large family of calcium-dependent phospholipid binding proteins known to undergo post-translational modifications such as phosphorylation. Physiological function of each annexin is still unclear since they may participate in signal transduction. We have tested the presence of annexins in a T cell line (Jurkat) and studied their phosphorylation by protein tyrosine kinases of the src family. Among annexins I, II, V and VI found in Jurkat cells, annexin VI was shown to be phosphorylated in vitro by p56lck and annexins I and II by p60src. We could not detect the phosphorylation of A-VI in vivo, even after cell stimulation. However, a 56-kDa phosphoprotein was found to be associated with A-VI after T cell activation. This 56-kDa protein shares some characteristics with p56lck.
Dubois
T
T
INSERM U332, Institut Cochin de Génétique Moléculaire, Université René Descartes, Paris, France.
Soula
M
M
Moss
S E
SE
Russo-Marie
F
F
Rothhut
B
B
D017317:Annexin A6 / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D001345:Autoradiography; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D010449:Peptide Mapping; D010748:Phosphopeptides / Q000302:isolation & purification; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D013601:T-Lymphocytes; D014407:Tumor Cells, Cultured
D017317:Annexin A6; D010748:Phosphopeptides; D010750:Phosphoproteins; D013462:Sulfur Radioisotopes; D008715:Methionine; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D016392:Proto-Oncogene Proteins pp60(c-src)