title: Maternal and umbilical serum concentrations of granulocyte colony-stimulating factor and its messenger RNA during clinical chorioamnionitis.
abstract: OBJECTIVE
  To determine whether women with chorioamnionitis developed elevated serum granulocyte colony-stimulating factor (G-CSF) concentrations and, if so, whether the G-CSF crossed to the fetus.
  
  
  METHODS
  We quantified G-CSF before, during, and after delivery and in the cord blood of 12 women with clinical chorioamnionitis and 12 matched controls. We also measured G-CSF messenger RNA (mRNA) transcripts in mononuclear cells isolated from maternal and cord blood at delivery.
  
  
  RESULTS
  At study entry, G-CSF concentrations were much higher in women with chorioamnionitis (1397 +/- 950 pg/mL, mean +/- standard error of mean) than in controls (17 +/- 5 pg/mL, P < .05). At delivery, concentrations had increased in both groups (2752 +/- 1100 pg/mL in the chorioamnionitis group, 165 +/- 61 pg/mL in controls; P < .05). After delivery, G-CSF concentrations had diminished to 839 +/- 594 pg/mL in women with chorioamnionitis and to 83 +/- 16 pg/mL in controls (P < .05). Concentrations in the cord blood were 2729 +/- 974 pg/mL for the chorioamnionitis group and 51 +/- 17 pg/mL for controls (P < .05). Granulocyte colony-stimulating factor mRNA transcripts were more abundant in women with chorioamnionitis than in controls but were scarce in all matched cord blood samples tested.
  
  
  CONCLUSIONS
  Serum G-CSF concentrations were elevated during clinical chorioamnionitis, and similar levels were found in maternal and cord serum. Because G-CSF mRNA levels were very low in cord mononuclear cells, the G-CSF in cord serum might be maternal in origin.
authors.affiliation: Department of Pediatrics, University of Florida College of Medicine, Gainesville, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: R K
authors.initials: RK
authors.lastname: Ohls
authors.forename: C
authors.initials: C
authors.lastname: Rosa
authors.forename: M
authors.initials: M
authors.lastname: Shah
authors.forename: D S
authors.initials: DS
authors.lastname: Richards
authors.forename: R D
authors.initials: RD
authors.lastname: Christensen
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002821:Chorioamnionitis / Q000097:blood / Q000276:immunology*; D036861:Delivery, Obstetric; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood* / Q000235:genetics; D006801:Humans; D016555:Matched-Pair Analysis; D008431:Maternal-Fetal Exchange; D049590:Postpartum Period; D011247:Pregnancy; D012333:RNA, Messenger / Q000097:blood*
chemical_list: D012333:RNA, Messenger; D016179:Granulocyte Colony-Stimulating Factor