Uses of flow cytometry in virology.
This article reviews some of the published applications of flow cytometry for in vitro and in vivo detection and enumeration of virus-infected cells. Sample preparation, fixation, and permeabilization techniques for a number of virus-cell systems are evaluated. The use of flow cytometry for multiparameter analysis of virus-cell interactions for simian virus 40, herpes simplex viruses, human cytomegalovirus, and human immunodeficiency virus and its use for determining the effect of antiviral compounds on these virus-infected cells are reviewed. This is followed by a brief description of the use of flow cytometry for the analysis of several virus-infected cell systems, including blue tongue virus, hepatitis C virus, avian reticuloendotheliosis virus, African swine fever virus, woodchuck hepatitis virus, bovine viral diarrhea virus, feline leukemia virus, Epstein-Barr virus, Autographa californica nuclear polyhedrosis virus, and Friend murine leukemia virus. Finally, the use of flow cytometry for the rapid diagnosis of human cytomegalovirus and human immunodeficiency virus in peripheral blood cells of acutely infected patients and the use of this technology to monitor patients on antiviral therapy are reviewed. Future prospects for the rapid diagnosis of in vivo viral and bacterial infections by flow cytometry are discussed.
McSharry
J J
JJ
Department of Microbiology, Immunology and Molecular Genetics, Albany Medical College, New York 12208.
D000914:Antibodies, Viral / Q000032:analysis / Q000302:isolation & purification; D000956:Antigens, Viral / Q000302:isolation & purification; D005434:Flow Cytometry; D005544:Forecasting; D006801:Humans; D013048:Specimen Handling; D013194:Staining and Labeling; D014773:Virology / Q000379:methods; D018406:Virus Physiological Phenomena; D014780:Viruses / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral; D000956:Antigens, Viral