Effect of glycerol on intracellular virus survival: implications for the clinical use of glycerol-preserved cadaver skin.
Glycerol has long been used for the preservation of skin allografts. The antimicrobial activity of glycerol has not been fully documented. This paper reports the results of an investigation of a model studying the effect of glycerol on the inactivation of intracellular viruses. Two viruses--herpes simplex type I (HSV-1) and poliovirus--were cultured within human dermal fibroblasts. These intracellular viruses were incubated with 50 per cent, 85 per cent and 98 per cent glycerol at 4 degrees C and 20 degrees C for 4 weeks. Each week, the cultures in glycerol and controls in fibroblast maintenance medium were assayed for virus infectivity by examining the ability of harvested viruses to infect further fibroblasts. At 4 degrees C, 85 per cent glycerol could not fully inactivate intracellular HSV-I or poliovirus even after 4 weeks; 98 per cent glycerol inactivated intracellular HSV-I (after 3 weeks) but could not fully inactivate intracellular poliovirus after 4 weeks. At 20 degrees C, 85 per cent glycerol inactivated intracellular HSV-I (within 1 week) but could not fully inactivate intracellular poliovirus after 4 weeks; 98 per cent glycerol inactivated intracellular HSV-I (within 1 week) and inactivated intracellular poliovirus (after 2 weeks). It is suggested that, on the basis of this study, glycerol can reduce intracellular virus infectivity but that its effects are very dependent on concentration, time and temperature such that we would recommend that allograft skin be exposed to 98 per cent glycerol for a minimum of at least 4 weeks at a minimum temperature of 20 degrees C before clinical use.
Marshall
L
L
Department of Virology, Northern General Hospital, Sheffield, UK.
Ghosh
M M
MM
Boyce
S G
SG
MacNeil
S
S
Freedlander
E
E
Kudesia
G
G
D002102:Cadaver; D002478:Cells, Cultured; D005347:Fibroblasts / Q000821:virology; D005990:Glycerol / Q000494:pharmacology*; D018259:Herpesvirus 1, Human / Q000187:drug effects / Q000254:growth & development*; D006801:Humans; D017955:Poliovirus / Q000187:drug effects / Q000254:growth & development*; D012867:Skin / Q000821:virology*; D016038:Skin Transplantation; D013696:Temperature; D014021:Tissue Preservation
D005990:Glycerol