A carboxylesterase from the parasitic nematode Ascaris suum homologous to the intestinal-specific ges-1 esterase of Caenorhabditis elegans.
We have identified a carboxylesterase in A. suum that appears to be the homolog of the gut-specific C. elegans ges-1 enzyme. The A. suum esterase was purified and its N-terminal sequence found to be 50% identical to the C. elegans ges-1 protein. We have used isoelectric focusing analysis to demonstrate that, unlike the C. elegans ges-1 esterase, the A. suum enzyme is not restricted to the gut but is expressed in a wide range of tissues.
Azzaria
M
M
Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
Henzel
W J
WJ
McGhee
J D
JD
D000595:Amino Acid Sequence; D000818:Animals; D017165:Ascaris suum / Q000201:enzymology*; D017173:Caenorhabditis elegans / Q000201:enzymology*; D029742:Caenorhabditis elegans Proteins; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry*; D005260:Female; D007422:Intestines / Q000201:enzymology*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D017386:Sequence Homology, Amino Acid
D029742:Caenorhabditis elegans Proteins; D002265:Carboxylic Ester Hydrolases; C095990:ges-1 protein, C elegans; D043182:Carboxylesterase