Differential display identifies developmentally regulated genes during somatic embryogenesis in eggplant (Solanum melongena L.).
Changes in gene expression patterns during early somatic embryogenesis of eggplant (Solanum melongena L.) were characterized by means of differential display. Eight products of polymerase chain reaction (PCR) derived from newly expressed mRNAs after four days of culture were identified and cloned. One clone, pTM002, was found to contain a 416 bp insert and identical to the proximal arbitrary primer at both ends. Based on the terminal sequences, reverse-transcription PCR (RT-PCR) was carried out to monitor the expression level of pTM002. The results indicated that the message of pTM002 was increased to correlate with the intensities obtained in the differential display. The significance of this method in investigation of differentially expressed genes is also discussed.
Momiyama
T
T
Department of Cell Biology, National Institute of Agrobiological Resources, Tsukuba, Japan.
Afele
J C
JC
Saito
T
T
Kayano
T
T
Tabei
Y
Y
Takaiwa
F
F
Takayanagi
K
K
Nishimura
S
S
D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D008969:Molecular Sequence Data; D010944:Plants / Q000196:embryology* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D018076:DNA, Complementary; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase