Tumor cell-enhanced sensitivity of vascular endothelial cells to photodynamic therapy.
Effective antitumor photodynamic therapy (PDT) may be related to damage of vasculature within the tumor. The purpose of this study was to determine if tumor cells secrete factors that stimulate proliferation of human umbilical vein endothelial cells (HUVEC) and result in enhanced sensitivity of HUVEC to aluminum-sulfonated phthalocyanine (AlSPc)-PDT. Three human tumor cell lines--pharyngeal squamous carcinoma, colonic carcinoma, and mammary carcinoma--were used in this study. Co-culture of HUVEC and either squamous carcinoma or colonic carcinoma, but not mammary carcinoma, significantly increased HUVEC proliferation and AlSPc-PDT mediated cell damage. In addition, supernatant from squamous carcinoma and colonic carcinoma cultures also stimulated HUVEC proliferation and sensitivity to AlSPc-PDT. Both supernatant and cell lysate from squamous carcinoma cells contained angiogenic factors consistent with basic and acidic fibroblast growth factors, as evidenced by Western blot analysis and BALB/c 3T3 fibroblast cell proliferation assays. Collectively, these results suggest that selected tumor cell lines produce angiogenic factors that induce HUVEC proliferation and subsequently enhance sensitivity to AlSPc-PDT.
Yang
Z
Z
Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, Knoxville 37901.
Lu
X
X
Frazier
D L
DL
Panjehpour
M
M
Breider
M A
MA
D016475:3T3 Cells / Q000187:drug effects; D000535:Aluminum / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D002277:Carcinoma / Q000378:metabolism* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology; D016220:Fibroblast Growth Factor 1 / Q000378:metabolism / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D007834:Lasers; D051379:Mice; D008807:Mice, Inbred BALB C; D009389:Neovascularization, Pathologic; D009942:Organometallic Compounds / Q000494:pharmacology; D010778:Photochemotherapy; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins / Q000166:cytology
D007211:Indoles; D009942:Organometallic Compounds; D011838:Radiation-Sensitizing Agents; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; C064331:aluminum phthalocyanine disulfonate; D000535:Aluminum