Exogenous expression of human granulocyte colony-stimulating factor receptor in a B-lineage acute lymphoblastic leukemia cell line: a possible model for mixed lineage leukemia.
We report evidence that the granulocyte colony-stimulating factor (G-CSF) receptor is present and may be functioning on blast cells from some patients with myeloid surface antigen positive (My+) acute lymphoblastic leukemia (ALL). In the present study, a human G-CSF receptor expression plasmid was transfected into a newly established B-lineage ALL cell line 'Tanoue' and its subclone 'ST' by lipofection to investigate whether expression of the G-CSF receptor and G-CSF stimulation would induce myeloid characteristics on myeloid surface antigen negative (My-) ALL cells. The G-CSF receptor became detectable on the transfected cells (GR-Tanoue and GR-ST), with dissociation constant values of 50-130 pmol/l, and maximal binding sites (Bmax of 77-6100 sites/cell on receptor binding assays. Short term culture with recombinant human G-CSF induced myeloid differentiation (a two to three-fold increase in CD33 and CD15 expression), and a moderate 3H-thymidine uptake (stimulation index, 1.75) only in the GR-ST clone no. 15 which expressed a high number of G-CSF receptors (Bmax, 6100 sites/cell). Our data show that (a) exogenous expression of the G-CSF receptor and G-CSF stimulation can induce myeloid characteristics on ALL cells; and (b) in the G-CSF receptor-expressing cells, there is a correlation between the number of G-CSF receptors and cell responsiveness to G-CSF in either proliferation or differentiation.
el-Sonbaty
S S
SS
Department of Pediatrics, Kumamoto University School of Medicine, Japan.
Tsuchiya
H
H
Watanabe
M
M
Hochito
K
K
Kunisada
T
T
Shimosaka
A
A
Matsuda
I
I
D002051:Burkitt Lymphoma / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002999:Clone Cells; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D016130:Immunophenotyping; D066298:In Vitro Techniques; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured
D016188:Receptors, Granulocyte Colony-Stimulating Factor; D016179:Granulocyte Colony-Stimulating Factor