title: Solution conformation of mu-selective dermorphin and delta-selective deltorphin-I in phospholipid micelles, studied by NMR spectroscopy and molecular dynamics simulations.
abstract: Complete proton resonance assignments of the naturally occurring mu-selective dermorphin (H-Tyr-D-Ala-Phe-Gly-Tyr-Pro-Ser-NH2) and delta-selective deltorphin-I (H-Tyr-D-Ala-Phe-Asp-Val-Val-Gly-NH2) were carried out by two-dimensional 1H-NMR techniques to investigate the conformational features in the membrane-mimetic micelles of perdeuterated dodecylphosphocholine. Fifty possible three-dimensional structures for respective peptides were generated by means of distance geometry calculations, all of which satisfy the proton-proton distances derived from NOE measurements within the allowable range, and 25 of them were subjected to the molecular dynamics simulations for 10 ps, in which the NOE distances were included as the energetic constraints. Although conformers simulated for dermorphin showed relatively large conformational variations because of the limited NOE data, most of them were characterized as an entirely folded structure bent at the Gly4 residue, where each of the N- and C-terminal tetrapeptides took an extended conformation. On the other hand, most conformations of deltorphin-I showed the common feature that the N-terminal Tyr-D-Ala-Phe-Asp and C-terminal Val-Val-Gly-NH2 sequences took respective folded conformations, and these were almost at right angles on the border of the Asp-Val sequence. These conformational characteristics are discussed in terms of the possible relationship with the mu/delta-opioid receptor selectivity.
authors.affiliation: Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Segawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ohno
authors.forename: M
authors.initials: M
authors.lastname: Doi
authors.forename: T
authors.initials: T
authors.lastname: Ishida
authors.forename: T
authors.initials: T
authors.lastname: Iwashita
mesh_terms: D000595:Amino Acid Sequence; D008081:Liposomes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry*; D018847:Opioid Peptides; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D011487:Protein Conformation; D011522:Protons; D011957:Receptors, Opioid / Q000378:metabolism; D012996:Solutions
chemical_list: D008081:Liposomes; D008823:Micelles; D009842:Oligopeptides; D018847:Opioid Peptides; D011522:Protons; D011957:Receptors, Opioid; D012996:Solutions; D010767:Phosphorylcholine; C060208:deltorphin I, Ala(2)-; C030058:dermorphin; C028810:dodecylphosphocholine