Enhanced adeno-associated virus vector expression by adenovirus protein-cationic liposome complex. A novel and high efficient way to introduce foreign DNA into endothelial cells.
Adeno-associated virus vector (AAV), a non-pathogenic integrating vector, can integrate on chromosome 19 in humans. But its gene transfer efficiency is quite low. In this study we combined adenovirus (Adv-5) capsid protein or the Fiber protein of Adv with liposome, termed adenosome (adenovirus protein-cationic liposome complex). This complex can bring AAV/CMV-LacZ to the endothelial cells, and improve the efficiency of gene transfer. It may be a novel, specific, stable and safe gene delivery system and will be widely used in human gene therapy.
Zhou
H
H
Cardiovascular Research Institute, Beijing Medical University.
Zeng
G
G
Zhu
X
X
Tang
J
J
Chen
G
G
Huang
Q
Q
Peng
T
T
Hu
B
B
D000260:Adenoviruses, Human / Q000235:genetics*; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D008081:Liposomes; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D014162:Transfection; D014764:Viral Proteins
D008081:Liposomes; D014764:Viral Proteins