Detection of hepatitis C virus (HCV) by reverse transcription-polymerase chain reaction (RT-PCR) (a note).
Since the majority of post-transfusion hepatitis cases are closely related to the HCV infections, the sensitive and specific detection of HCV-RNA is a central issue both in blood transfusion and in follow-up of hepatitis patients. This leaflet provides the technical details of HCV-RT-PCR.
Falus
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Department of Biology, Semmelweis University Medical School, Budapest, Hungary.
Hajnal
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D001483:Base Sequence; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000097:blood / Q000302:isolation & purification
D012367:RNA, Viral