title: Molecular characterization of FrpB, the 70-kilodalton iron-regulated outer membrane protein of Neisseria meningitidis.
abstract: The structural gene encoding the 70-kDa outer membrane protein FrpB of Neisseria meningitidis was cloned and sequenced. A mutant lacking FrpB was constructed. No difference in iron utilization between the mutant and the parental strain was observed. A minor effect of the mutation on serum resistance was observed. A topology model for FrpB in the outer membrane is proposed.
authors.affiliation: Department of Molecular Cell Biology, Utrecht University, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Pettersson
authors.forename: A
authors.initials: A
authors.lastname: Maas
authors.forename: D
authors.initials: D
authors.lastname: van Wassenaar
authors.forename: P
authors.initials: P
authors.lastname: van der Ley
authors.forename: J
authors.initials: J
authors.lastname: Tommassen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D007501:Iron / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D009345:Neisseria meningitidis / Q000235:genetics / Q000378:metabolism*; D011817:Rabbits
chemical_list: D001425:Bacterial Outer Membrane Proteins; C093120:FrpB protein, bacteria; D007501:Iron