Increased eosinophil oxidative metabolism by treatment with soluble intercellular adhesion molecule-1.
Adhesion molecules may play an important role not only in adherence of inflammatory cells (particularly eosinophils) to an inflamed focus but also in activation of these cells. It is therefore of interest to evaluate eosinophil activation via intercellular adhesion molecule-1 (ICAM-1) and the beta 2-integrin family, namely CR3 (Mac-1), lymphocyte function-associated antigen (LFA)-1 alpha and LFA-1 beta, which are ligands for ICAM-1. Reactive oxygen species generated by eosinophils have also been considered capable of causing airway injury at the inflamed focus. This study examined the effect of recombinant soluble ICAM-1 and its ligands on eosinophil-induced radical oxygen products in terms of luminol-dependent chemiluminescence. Recombinant soluble ICAM-1 augmented eosinophil oxidative metabolism. It was concluded that signaling via adhesion molecules might play an important role in the pathogenesis of allergic inflammation through activation of eosinophils, e.g. an increase in oxidative metabolism.
Chihara
J
J
Fourth Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.
Kakazu
T
T
Higashimoto
I
I
Yamamoto
T
T
Kurachi
D
D
Nakajima
S
S
D001249:Asthma / Q000503:physiopathology; D002448:Cell Adhesion; D004804:Eosinophils / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology; D016897:Respiratory Burst; D015398:Signal Transduction; D012995:Solubility
D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1