title: HPV16 E7 oncoprotein induces expression of a 110 kDa heat shock protein.
abstract: Heat shock protein genes are induced by various kinds of stress. Besides stress, the heat shock family gene hsp70 has been shown to be induced by growth-stimulating agents such as the DNA virus oncoproteins and serum. Here, we report cloning of a novel cDNA that encodes a 100 kDa heat shock protein-related polypeptide as a human papillomavirus oncoprotein E7-inducible gene. E7 induces expression of this heat shock protein at the level of RNA synthesis. Moreover, the induction of this heat shock protein-mRNA was dependent on the conserved region 2 of the E7 protein, which is essential for binding to the proteins of the retinoblastoma family.
authors.affiliation: Department of Biochemistry (M/C 536), University of Illinois at Chicago 60612, USA.
authors.forename: A
authors.initials: A
authors.lastname: Morozov
authors.forename: J
authors.initials: J
authors.lastname: Subjeck
authors.forename: P
authors.initials: P
authors.lastname: Raychaudhuri
mesh_terms: D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D050957:HSP110 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000235:genetics*; D018869:Heat-Shock Response; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D050957:HSP110 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C097205:HSPH1 protein, human; C490783:Hsp105 protein, mouse; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C059731:oncogene protein E7, Human papillomavirus type 16