title: Alcohol dehydrogenase of class I: kiwi liver enzyme, parallel evolution in separate vertebrate lines, and correlation with 12S rRNA patterns.
abstract: Alcohol dehydrogenase class I from kiwi liver has been purified, analyzed, and compared with that of other alcohol dehydrogenases. The results show that several avian and mammalian forms of the enzyme exhibit parallel evolutionary patterns in two independent lineages of a single protein, establishing a pattern in common. Furthermore, the data correlate the enzyme evolutionary pattern with that of 12S rRNA. Biologically, the patterns complement those on ratite and other avian relationships. Functionally, the enzyme has a low Km with ethanol and a branched-chain residue at position 141, like the mammalian enzymes but in contrast to the other characterized ratite enzyme (with Ala-141 and a higher Km). This pattern of natural variability suggests a frequent but not fully complete correlation between a large residue size at position 141 and tight ethanol binding.
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Hjelmqvist
authors.forename: M
authors.initials: M
authors.lastname: Metsis
authors.forename: H
authors.initials: H
authors.lastname: Persson
authors.forename: J O
authors.initials: JO
authors.lastname: Höög
authors.forename: J
authors.initials: J
authors.lastname: McLennan
authors.forename: H
authors.initials: H
authors.lastname: Jörnvall
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000145:classification / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D005075:Biological Evolution; D001717:Birds / Q000235:genetics*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012313:RNA / Q000737:chemistry; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; D012313:RNA; D000426:Alcohol Dehydrogenase