Modulation of APRT transcription by altering spacing between cis-regulatory elements.
Three regulatory regions on the promoter of CHO adenine phosphoribosyltransferase gene (APRT) were identified. Spacing constraints between these regions were analyzed. With normal spacing, region II (-33 to +19), which separates regions I (-101 to -53) and III (+56 to +85), is critical for APRT transcription. However, when regions I and III are artificially placed in proximity to each other (region II deleted), they are able to drive transcription as efficiently as the wild-type APRT promoter. Neither region I nor III alone is sufficient for efficient transcription. As the spacing between the two regions increases, the transcription decreases. Region I may activate transcription in two ways: through a stringent sequence-specific manner (as in the transcription mediated by regions I and III) and through a manner with relaxed requirement for sequence specificity (as in the transcription from the wild-type APRT promoter).
She
B R
BR
Department of Biology, Indiana University, Bloomington 47405, USA.
Schaley
J E
JE
Taylor
M W
MW
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics*; D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D006224:Cricetinae; D017353:Gene Deletion; D005809:Genes, Regulator / Q000235:genetics*; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics
D012313:RNA; D000228:Adenine Phosphoribosyltransferase