Microdissection based cloning of a translocation breakpoint in a human malignant melanoma.
Chromosome translocations in human malignancies have identified the genomic location of several important growth-regulatory sequences (e.g., cellular oncogenes and suppressor genes). Melanomas are characterized by recurring chromosome alterations, including deletion or translocations of the long arm of chromosome 6 (6q). This report details our efforts to clone the t(1;6)(q21;q14) breakpoint in a malignant melanoma to further our understanding of the biology of these tumors. The strategy utilized combined microdissection of the translocation chromosome, development and characterization of a DNA microclone library, isolation of cosmids and YACs from the breakpoint region, ordering of clones by two-color metaphase/interphase fluorescence in situ hybridization, and finally, identification of a YAC spanning the translocation breakpoint. By analogy to other tumor systems, molecular examination of the chromosome 6 breakpoint may provide insight into the pathobiology of this important neoplasm.
Zhang
J
J
Laboratory of Cancer Genetics, National Center for Human Genome Research, NIH, Bethesda, Maryland 20892, USA.
Cui
P
P
Glatfelter
A A
AA
Cummings
L M
LM
Meltzer
P S
PS
Trent
J M
JM
D000328:Adult; D001483:Base Sequence; D002869:Chromosome Aberrations / Q000235:genetics*; D025063:Chromosome Disorders; D018244:Chromosomes, Artificial, Yeast; D002878:Chromosomes, Human, Pair 1; D002896:Chromosomes, Human, Pair 6; D003001:Cloning, Molecular; D003360:Cosmids; D017931:DNA Primers / Q000737:chemistry; D004210:Dissection; D005260:Female; D005819:Genetic Markers; D015698:Genomic Library; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008545:Melanoma / Q000235:genetics*; D008969:Molecular Sequence Data; D014178:Translocation, Genetic / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers