title: Purification and characterization of an acid phosphatase from Arachis hypogaea.
abstract: An acid phosphatase from Arachis hypogaea (peanuts) has been purified. The electrophoretically homogeneous enzyme preparation is free of any phophodiesterase activity. The enzyme has a molecular weight of 120,000. Among the various phosphomonoesters tested, p-nitrophenylphosphate was found to be its most effective substrate. The Km for p-nitrophenylphosphate was 1.21 mM at pH 5.0 and 25 degrees C. The enzyme was thermostable and did not loose activity after 1 hr at 50 degrees C.
authors.affiliation: Chemistry Department I.I.T. Delhi, India.
authors.forename: R
authors.initials: R
authors.lastname: Srivastava
authors.forename: Y S
authors.initials: YS
authors.lastname: Rajput
authors.forename: S K
authors.initials: SK
authors.lastname: Khare
authors.forename: R
authors.initials: R
authors.lastname: Tyagi
authors.forename: M N
authors.initials: MN
authors.lastname: Gupta
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry / Q000302:isolation & purification* / Q000502:physiology; D010367:Arachis / Q000737:chemistry / Q000201:enzymology*; D005591:Chemical Fractionation; D002845:Chromatography / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D004586:Electrophoresis; D005459:Fluorides / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009596:Nitrophenols / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D009596:Nitrophenols; D009943:Organophosphorus Compounds; C008644:nitrophenylphosphate; D000135:Acid Phosphatase; D005459:Fluorides