title: Membrane-associated interleukin-1 on macrophages stimulated with Actinobacillus actinomycetemcomitans lipopolysaccharide induces osteoclastic bone resorption in vivo.
abstract: The effect of paraformaldehyde-fixed murine macrophage P388D1 cells stimulated with Actinobacillus actinomycetemcomitans Y4 lipopolysaccharide (membrane-associated interleukin-1) on osteoclastic bone resorption was investigated. Both the number of osteoclasts and the bone resorption surfaces increased considerably along the inside of the marrow space of mouse calvaria when membrane-associated interleukin-1 mediated by A. actinomycetemcomitans Y4 lipopolysaccharide were injected into the subcutaneous tissues overlying the central calvaria of 4-week-old C3H/HeJ mice. Membrane-associated interleukin-1 caused hypercalcaemia when injected into C3H/HeJ mice twice a day for 2 days. The calvaria of C3H-HeJ mice injected with membrane-associated interleukin-1 contained less mineral than those of the control mice. These results suggest that membrane-associated interleukin-1 on macrophages stimulated with A. actinomycetemcomitans Y4 lipopolysaccharide plays an important role in inflammatory osteoclastic bone resorption in periodontal diseases.
authors.affiliation: Department of Oral Science, National Institute of Health, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nishihara
authors.forename: Y
authors.initials: Y
authors.lastname: Ishihara
authors.forename: T
authors.initials: T
authors.lastname: Koseki
authors.forename: E A
authors.initials: EA
authors.lastname: Boutsi
authors.forename: H
authors.initials: H
authors.lastname: Senpuku
authors.forename: N
authors.initials: N
authors.lastname: Hanada
mesh_terms: D016976:Aggregatibacter actinomycetemcomitans / Q000276:immunology*; D000818:Animals; D001862:Bone Resorption; D002118:Calcium / Q000097:blood; D002460:Cell Line; D004396:Coloring Agents; D007279:Injections, Subcutaneous; D007375:Interleukin-1 / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008262:Macrophage Activation; D008264:Macrophages / Q000502:physiology*; D051379:Mice; D008809:Mice, Inbred C3H; D010010:Osteoclasts / Q000502:physiology*; D012886:Skull / Q000473:pathology
chemical_list: D004396:Coloring Agents; D007375:Interleukin-1; D008070:Lipopolysaccharides; D002118:Calcium