Cloning and in vivo expression of the pig MyoD gene.
The complete sequence of the pig MyoD gene has been determined through the isolation and characterization of a 17 kb genomic clone. The deduced amino acid sequence shows relative conservation of about 90% with the MyoD sequences determined in other mammalian species, though there are several non-conservative changes scattered throughout the C-terminus. In situ hybridization on pig embryos (20-30 days post coitum) showed that MyoD expression was confined to myotomes and skeletal muscle masses.
Chang
K C
KC
Department of Veterinary Basic Sciences, Royal Veterinary College, University of London, UK.
Fernandes
K
K
Chantler
P D
PD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004622:Embryo, Mammalian; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D017570:MyoD Protein / Q000096:biosynthesis* / Q000235:genetics*; D051381:Rats; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012756:Sheep; D013552:Swine
D017570:MyoD Protein