title: A specifically apical sub-membrane intermediate filament cytoskeleton in non-brush-border epithelial cells.
abstract: Although many pieces of evidence support the notion of a role for the cytoskeleton in epithelial polarization, no cytoskeletal component has been found to be specifically apical, except for some actin-binding proteins. Here we report the apical distribution of a 53 kDa cytokeratin. Furthermore, this cytokeratin co-purified with biotinylated apical plasma membrane proteins in high density complexes. Differential biotinylation of the basolateral domain showed that the 53 kDa protein is mainly attached to the apical membrane, although a companion 58 kDa protein attaches to both apical and basolateral membrane proteins. Immunoprecipitation experiments indicated that a number of apical components are directly or indirectly linked to the 53 kDa protein. These results indicate the existence of a terminal web-like structure in non-brush-border cells, which attaches to the apical domain and may play a role in apical polarization, especially during the acquisition of polarity from non-polarized cellular stages.
authors.affiliation: Instituto de Investigaciones Bioquímicas, Fundación Campomar, Buenos Aires, Argentina.
authors.forename: M L
authors.initials: ML
authors.lastname: Rodríguez
authors.forename: M
authors.initials: M
authors.lastname: Brignoni
authors.forename: P J
authors.initials: PJ
authors.lastname: Salas
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D016764:Cell Polarity; D003599:Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure*; D004285:Dogs; D004848:Epithelium / Q000378:metabolism* / Q000648:ultrastructure*; D007382:Intermediate Filaments / Q000378:metabolism / Q000648:ultrastructure; D007633:Keratins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism / Q000648:ultrastructure; D008970:Molecular Weight
chemical_list: D008565:Membrane Proteins; D007633:Keratins