Improved direct PCR screen for bacterial colonies: wooden toothpicks inhibit PCR amplification.
Lee
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Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA.
Cooper
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D004269:DNA, Bacterial / Q000302:isolation & purification*; D005784:Gene Amplification; D019384:Nucleic Acid Synthesis Inhibitors; D010969:Plastics; D016133:Polymerase Chain Reaction / Q000379:methods*; D018894:Reverse Transcriptase Inhibitors; D019914:Taq Polymerase; D014934:Wood
D004269:DNA, Bacterial; D019384:Nucleic Acid Synthesis Inhibitors; D010969:Plastics; D018894:Reverse Transcriptase Inhibitors; D019914:Taq Polymerase