title: Peptide YY inhibits exocrine pancreatic secretion in isolated perfused rat pancreas by Y1 receptors.
abstract: Peptide YY (PYY) inhibits exocrine pancreatic secretion in several species. Two receptors, Y1 and Y2, are known to mediate PYY actions. While PYY 1-36 binds equally to both receptor subtypes, a second endogenous form of PYY, PYY 3-36, selectively activates Y2 receptors. The importance of Y receptor subtypes for inhibition of exocrine pancreatic secretion by PYY is unknown. We studied the effects of PYY 1-36 on cholecystokinin octapeptide (CCK-8)-stimulated amylase secretion in an isolated perfused rat pancreas model. To characterize functionally the receptors involved we determined the effects of a Y1-selective agonist, [Pro34]PYY; a Y2 selective agonist, PYY 3-36; and neuropeptide Y (NPY) in this model. PYY 1-36 significantly inhibited stimulated amylase secretion in the denervated rat pancreas. [Pro34]PYY and NPY both inhibited exocrine pancreatic secretion as potently as PYY 1-36. Contrary to that, the Y2 selective agonist, PYY 3-36, was inactive. We conclude that PYY inhibits exocrine pancreatic secretion in this extrinsically denervated rat pancreas model by Y1 receptors.
authors.affiliation: Department of Gastroenterology, University of Essen, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Grandt
authors.forename: J
authors.initials: J
authors.lastname: Siewert
authors.forename: B
authors.initials: B
authors.lastname: Sieburg
authors.forename: O
authors.initials: O
authors.lastname: al Tai
authors.forename: M
authors.initials: M
authors.lastname: Schimiczek
authors.forename: H
authors.initials: H
authors.lastname: Goebell
authors.forename: P
authors.initials: P
authors.lastname: Layer
authors.forename: V E
authors.initials: VE
authors.lastname: Eysselein
authors.forename: J R
authors.initials: JR
authors.lastname: Reeve
authors.forename: M K
authors.initials: MK
authors.lastname: Müller
mesh_terms: D000681:Amylases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D019894:Peptide YY; D010455:Peptides / Q000494:pharmacology*; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D017476:Receptors, Neuropeptide Y / Q000187:drug effects*; D012844:Sincalide / Q000187:drug effects*
chemical_list: D010455:Peptides; D017476:Receptors, Neuropeptide Y; D019894:Peptide YY; D000681:Amylases; D012844:Sincalide