title: Expression of cellular fibronectin and tenascin in the rabbit cornea after excimer laser photorefractive keratectomy: a 12 month study.
abstract: An indirect immunohistochemical technique was used to monitor the expression of cellular fibronectin (cFN) and tenascin (TN) in the rabbit cornea after photorefractive keratectomy (PRK) in a 1 year follow up study. Rabbits received a 5.0 D myopic PRK, and were killed 3 days, 1, 3, 6, or 12 months after the operation. In most corneas, secondary epithelial defects appeared after the primary healing (mean 6.3 (SD 1.2) days). Corneal haze appeared a few weeks after PRK and was observed throughout the follow up. Three days after wounding an immunoreaction for cFN was observed as a bright narrow subepithelial line, but no immunoreaction for TN could be seen in the anterior third of the corneal stroma. However, at 1-6 months a similar location of immunoreactions for both cFN and TN was observed. Both were found in the anterior stroma at depths of 30-50 microns. At 12 months, only a trace of cFN immunoreaction but no TN immunoreaction could be discerned. Our results suggest that subepithelial scar tissue contains both cFN and TN up to 12 months.
authors.affiliation: University of Helsinki, Department of Ophthalmology, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Latvala
authors.forename: K
authors.initials: K
authors.lastname: Tervo
authors.forename: R
authors.initials: R
authors.lastname: Mustonen
authors.forename: T
authors.initials: T
authors.lastname: Tervo
mesh_terms: D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis*; D003315:Cornea / Q000378:metabolism / Q000601:surgery*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D005260:Female; D005353:Fibronectins / Q000096:biosynthesis*; D005455:Fluorescent Antibody Technique; D053685:Laser Therapy; D008297:Male; D011817:Rabbits; D019063:Tenascin; D013997:Time Factors; D014945:Wound Healing
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D019063:Tenascin