Divergence of a flagellin protein in Serratia marcescens.
A gene (hag) encoding the flagellin (Fla) protein was cloned from Serratia marcescens (Sm) 8000, the wild-type strain of Sr41. The hag gene codes for a 348-amino-acid (aa) protein of 36.7 kDa. The predicted aa sequence showed 79% homology compared with the Fla of Sm 274 which has been reported previously [Harshey et al., Gene 79 (1989) 1-8]. Dot-matrix analysis of the Sm 8000 Fla showed that the N- and C-terminal regions of this protein were highly similar to those of other bacterial Fla. However, the aa sequence of the middle portion was quite different from that of a variant strain of the same species, Sm 274.
Akatsuka
H
H
Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
Kawai
E
E
Omori
K
K
Shibatani
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D005408:Flagellin / Q000737:chemistry / Q000235:genetics*; D005798:Genes, Bacterial; D014644:Genetic Variation; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D012706:Serratia marcescens / Q000235:genetics*
D017931:DNA Primers; D011994:Recombinant Proteins; D005408:Flagellin