title: Coxsackievirus B3 adapted to growth in RD cells binds to decay-accelerating factor (CD55).
abstract: A coxsackievirus B3 (CB3) isolate adapted to growth in RD cells shows an alteration in cell tropism as a result of its capacity to bind a 70-kDa cell surface molecule expressed on these cells. We now show that this molecule is the complement regulatory protein, decay-accelerating factor (DAF) (CD55). Anti-DAF antibodies prevented CB3 attachment to the cell surface. Radiolabeled CB3 adapted to growth in RD cells bound to CHO cells transfected with human DAF, whereas CB3 (strain Nancy), the parental strain, did not bind to DAF transfectants. These results indicate that growth of CB3 in RD cells selected for a virus strain that uses DAF for cell surface attachment.
authors.affiliation: Laboratory of Infectious Diseases, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
authors.forename: J M
authors.initials: JM
authors.lastname: Bergelson
authors.forename: J G
authors.initials: JG
authors.lastname: Mohanty
authors.forename: R L
authors.initials: RL
authors.lastname: Crowell
authors.forename: N F
authors.initials: NF
authors.lastname: St John
authors.forename: D M
authors.initials: DM
authors.lastname: Lublin
authors.forename: R W
authors.initials: RW
authors.lastname: Finberg
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D018958:CD55 Antigens; D029822:Enterovirus B, Human / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D066298:In Vitro Techniques; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D017384:Sequence Deletion; D013329:Structure-Activity Relationship
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018958:CD55 Antigens; D008562:Membrane Glycoproteins; D011991:Receptors, Virus; D011994:Recombinant Proteins