title: Quantitative and qualitative analysis of exogenous gene expression by the S1 nuclease protection assay.
abstract: A method for determining the beginning and end of gene transcripts along with the position of intron/exon boundaries by S1 nuclease protection is described. Probe strategies and hybridization conditions are considered in detail. Under conditions of probe excess the method is quantitative.
authors.affiliation: Developmental Biology Research Centre, King's College, University of London, UK.
authors.forename: M E
authors.initials: ME
authors.lastname: Walmsley
authors.forename: R K
authors.initials: RK
authors.lastname: Patient
mesh_terms: D001709:Biotechnology; D015342:DNA Probes / Q000302:isolation & purification; D005091:Exons; D015870:Gene Expression; D005821:Genetic Techniques; D007438:Introns; D015336:Molecular Probe Techniques; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
chemical_list: D015342:DNA Probes; D012313:RNA; D015719:Single-Strand Specific DNA and RNA Endonucleases