Identification and characterization of Schistosoma mansoni antigens recognized by T and B lymphocytes of humans with early active intestinal and/or urinary schistosomiasis.
Schistosome antigens selected as vaccine candidates should induce in the majority of humans T and B cell-mediated immunity that results in protection against infection. As a first step towards the identification of such antigens, we attempted to define and characterize the soluble adult Schistosoma mansoni worm antigen (SAWA) bands that are recognized by serum antibodies and/or peripheral blood mononuclear cells of Egyptian children with early active S. mansoni and/or S. haematobium infection. Considerable inter-subject variation was observed in the SAWA bands recognized by antibodies and T lymphocytes, as demonstrated by Western blotting and T cell Western assays, respectively. The humoral response rate for the separated SAWA bands varied between 0% and 88% of infected subjects. The bands of 153, 144, 38 and 32 kDa reacted with the sera of 60 to 88% of infected subjects but not with the sera of uninfected controls. The bands of 144, 38, 32 and 18 kDa elicited proliferative responses in the lymphocytes of 42-63% of infected subjects. It was thus concluded that the SAWA bands of 144, 38 and 32 kDa are likely to carry T and B cell epitopes that could stimulate immune responses in a majority of individuals. The selected bands (144, 38 and 32 kDa) were found to include glycoproteins containing D-mannopyranosyl or glycosyl residues, and respectively 62.5, 46 and 55% amino acids by weight. The amino acid molar ratios of these bands were completely different.
al-Sherbiny
M
M
Zoology Department, Faculty of Science, Cairo University, Egypt.
el Ridi
R
R
Guirguis
N I
NI
Dean
D A
DA
D000293:Adolescent; D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry / Q000302:isolation & purification*; D001402:B-Lymphocytes / Q000276:immunology; D002648:Child; D000939:Epitopes / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology; D015801:Helminth Proteins / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D008297:Male; D051379:Mice; D008970:Molecular Weight; D012550:Schistosoma mansoni / Q000276:immunology*; D012553:Schistosomiasis haematobia / Q000276:immunology* / Q000517:prevention & control; D012555:Schistosomiasis mansoni / Q000276:immunology* / Q000517:prevention & control; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology; D014612:Vaccines / Q000302:isolation & purification
D000947:Antigens, Helminth; D000939:Epitopes; D006023:Glycoproteins; D015801:Helminth Proteins; D014612:Vaccines