title: A gene responsible for vegetative incompatibility in the fungus Podospora anserina encodes a protein with a GTP-binding motif and G beta homologous domain.
abstract: The het-e-1 gene of the fungus Podospora anserina is responsible for vegetative incompatibility through specific interactions with different alleles of the unlinked gene, het-c. Coexpression of two incompatible genes triggers a cell death reaction that prevents heterokaryon formation. The het-e1 allele has been cloned to get information on the function of the locus. It encodes a putative 1356-amino-acid polypeptide that displays two sequence motifs that have not yet been reported to be present on a single polypeptide. They are a GTP-binding domain and a repeated region that shares similarity with that of the beta-transducin. Contrary to other members of the beta-transducin family, sequence conservation between the repeated units is very strong and the number of repeats is different in wild-type het-e alleles.
authors.affiliation: Laboratoire de Génétique, UPR CNRS 9026, Université de Bordeaux II, Talence, France.
authors.forename: S
authors.initials: S
authors.lastname: Saupe
authors.forename: B
authors.initials: B
authors.lastname: Turcq
authors.forename: J
authors.initials: J
authors.lastname: Bégueret
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001203:Ascomycota / Q000235:genetics* / Q000254:growth & development; D005656:Fungal Proteins / Q000235:genetics*; D019204:GTP-Binding Proteins; D005800:Genes, Fungal; D015698:Genomic Library; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D012098:Reproduction / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015293:Transducin / Q000031:analogs & derivatives* / Q000235:genetics
chemical_list: D005656:Fungal Proteins; C095738:HET-E1 protein, Podospora anserina; D019204:GTP-Binding Proteins; D015293:Transducin