Enolase and the arsonomethyl analogue of 2-phosphoglycerate.
(RS)-3-Arsono-2-(hydroxymethyl)propionic acid was synthesized by the action of alkaline arsenite on 3-bromo-2-(bromomethyl)propionic acid. It is a substrate for yeast enolase (EC 4.2.1.11) with a Km of 6.5 mM (for 2-phospho-D-glycerate Km = 0.08 mM). The catalytic constant of the enzyme with the arsonomethyl analogue is 230 times lower than with 2-phosphoglycerate.
Chawla
S
S
Department of Biochemistry, University of Cambridge, UK.
Dixon
H B
HB
D001152:Arsenicals / Q000138:chemical synthesis* / Q000378:metabolism; D005988:Glyceric Acids / Q000138:chemical synthesis* / Q000378:metabolism; D007700:Kinetics; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013056:Spectrophotometry, Ultraviolet
C094717:3-arsono-2-(hydroxymethyl)propanoic acid; D001152:Arsenicals; D005988:Glyceric Acids; C008885:2-phosphoglycerate; D010751:Phosphopyruvate Hydratase