On the mechanism of thyroid hormone-induced respiratory burst activity in rat polymorphonuclear leukocytes.
Administration of single doses of 0.1 mg L-triiodothyronine (T3)/kg for 3 consecutive days to fed rats produced a drastic increase in the respiratory burst activity of isolated polymorphonuclear leukocytes (PMN), stimulated with serum-opsonized zymosan. This effect was evidenced by the 3.8-fold increment in the integrated chemiluminescence, and seems to be primarily related to the enhanced activity of NADPH oxidase elicited by T3 treatment, with the observed higher myeloperoxidase activity playing a contributory role. In these conditions, hyperthyroidism determines a net enhancement in the oxidant capacity of PMN, as the increased rate of O2.- generation found occurs in the absence of changes in the activity of superoxide dismutase.
Fernández
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V
Department of Biochemistry, Faculty of Medicine, University of Chile, Santiago.
Videla
L A
LA
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D066298:In Vitro Techniques; D008163:Luminescent Measurements; D009247:NADH, NADPH Oxidoreductases / Q000097:blood; D019255:NADPH Oxidases; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016897:Respiratory Burst / Q000187:drug effects* / Q000502:physiology; D013481:Superoxides / Q000097:blood; D014284:Triiodothyronine / Q000494:pharmacology*; D015054:Zymosan / Q000494:pharmacology
D014284:Triiodothyronine; D013481:Superoxides; D015054:Zymosan; D009247:NADH, NADPH Oxidoreductases; D019255:NADPH Oxidases