Effects of high levels of selenite and Escherichia coli on enrichment of Salmonella and detection of Salmonella by polymyxin-cloth enzyme immunoassay.
The effect of various concentrations of selenite on the recovery of Salmonella typhimurium and Escherichia coli was examined in a rich medium containing 0.5% cholate. For heat-injured S. typhimurium, longer preincubation periods were required for recovery in higher concentrations of selenite. When 0.7% selenite was added to 6-h preincubated cultures of heat-injured cells, S. typhimurium was fully recovered while E. coli, whether heat-injured or uninjured, did not recover when present at numbers at less than 2 x 10(7) colony forming units (CFU). The polymyxin-cloth enzyme immunoassay detected S. typhimurium grown from a small number (as low as 1 CFU) of cells in the presence of a large excess of E. coli.
Chen
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Department of Biology, Carleton University, Ottawa, Ontario, Canada.
Hayashi
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Yamazaki
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H
D019826:Cholic Acid; D002793:Cholic Acids; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification*; D006358:Hot Temperature; D007124:Immunoenzyme Techniques; D012475:Salmonella / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification*; D018038:Sodium Selenite / Q000494:pharmacology*; D013997:Time Factors
D002793:Cholic Acids; D003470:Culture Media; D019826:Cholic Acid; D018038:Sodium Selenite