title: E-cadherin peptide sequence recognition by anti-E-cadherin antibody.
abstract: Cadherins are calcium dependent glycoproteins involved in homophilic cell-cell adhesion. To further elucidate the interaction between cadherins we have developed an immobilized peptide ELISA to quickly scan the extracellular domains of E-cadherin. Peptides displaying antigenic reactivity to anti-E-cadherin antibody are presumably on the surface and thus may be involved in cadherin-cadherin interaction. We have found three peptides from the EC-1 domain which are recognized by the anti-E-cadherin antibody. These peptides are in the two important regions of the EC-1 domain that was deduced from its secondary structure.
authors.affiliation: Department of Pharmaceutical Chemistry, University of Kansas, Lawrence 66045, USA.
authors.forename: K L
authors.initials: KL
authors.lastname: Lutz
authors.forename: T J
authors.initials: TJ
authors.lastname: Siahaan
mesh_terms: D000409:Alanine; D000595:Amino Acid Sequence; D001666:Binding Sites, Antibody; D015153:Blotting, Western; D015820:Cadherins / Q000737:chemistry* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis / Q000737:chemistry*; D006735:Horseradish Peroxidase; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology
chemical_list: D015820:Cadherins; D000939:Epitopes; D010446:Peptide Fragments; D011994:Recombinant Proteins; D006735:Horseradish Peroxidase; D000409:Alanine