Subverting lymph node trafficking by treatment with the Mel-14 monoclonal antibody to L-selectin does not prevent an effective host response to Sendai virus.
A single 250-micrograms dose of the Mel-14 mAb to L-selectin greatly diminished the extent of L-selectin expression on lymphocytes and decreased (60 to 90%) the massive cellular recruitment to the cervical and mediastinal lymph nodes that follows intranasal infection of naive C57BL/6 mice with Sendai virus. The numbers of CD8+ CTL precursors in the mediastinal lymph nodes were considerably reduced on day 7, when compared with virus-infected mice given a control rat IgG2a, but potent CTL effectors were present in the lungs of both groups by day 10 after infection, and the overall magnitude of CTL precursor generation was not obviously compromised. The early dominance of Sendai virus-specific IgM Ab-forming cells was prolonged in the Mel-14-treated mice, whereas plasma cells producing virus-specific IgA were abnormally prominent in the lymph nodes but not in the spleen. The kinetics of virus-specific Ab-forming cells generation and the serum Ab response for the various IgG isotypes were also delayed. Thus, though L-selectin is clearly important for the localization of naive lymphocytes to regional lymph nodes, the Mel-14-treated mouse can still deal effectively with a virus that causes productive infection only in the respiratory tract. The spleen, where L-selectin does not determine lymphocyte trafficking, is a major site for the compensatory T cell and B cell responses.
Hou
S
S
Department of Biology, University of California San Diego, La Jolla 92093, USA.
Hyland
L
L
Bradley
L M
LM
Watson
S R
SR
Doherty
P C
PC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000166:cytology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000821:virology; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D005260:Female; D019041:L-Selectin; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D018184:Paramyxoviridae Infections / Q000517:prevention & control; D013154:Spleen / Q000166:cytology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D015815:Cell Adhesion Molecules; D019041:L-Selectin