title: Cystic fibrosis gene mutations do not affect sperm function during in vitro fertilization with micromanipulation for men with bilateral congenital absence of vas deferens.
abstract: OBJECTIVE
  To assess the effects of cystic fibrosis transmembrane-conductance regulator (CFTR) gene mutations on sperm function and fertility in men with bilateral congenital absence of the vas deferens.
  
  
  METHODS
  Prospective.
  
  
  METHODS
  Division of urologic microsurgery and associated hospital-based IVF unit.
  
  
  METHODS
  Fertilization and pregnancy rates.
  
  
  METHODS
  Men referred to our fertility unit for treatment of bilateral congenital absence of the vas deferens, using sperm surgically retrieved from the epididymis with IVF and micromanipulation.
  
  
  RESULTS
  Of 45 men with bilateral congenital absence of the vas, 54% (19/35) tested were found to be carriers of CFTR gene mutations, with one compound heterozygote. Epididymal sperm from men affected with CFTR mutations fertilized 19% (29/152) of oocytes, whereas men without mutations fertilized 22% (44/204) of oocytes. Pregnancy rates (PRs) were 36% (5/14) for cycles involving men with CFTR mutations and 33% (5/15) for other patients with congenital absence of the vas deferens but without detectable CFTR mutations.
  
  
  CONCLUSIONS
  The presence of detectable CFTR mutations does not affect fertilization rates or PRs for men with bilateral congenital absence of the vas deferens when IVF and micromanipulation are applied.
authors.affiliation: James Buchanan Brady Foundation, Department of Urology, New York Hospital-Cornell Medical Center, New York 10021, USA.
authors.forename: P N
authors.initials: PN
authors.lastname: Schlegel
authors.forename: J
authors.initials: J
authors.lastname: Cohen
authors.forename: M
authors.initials: M
authors.lastname: Goldstein
authors.forename: M
authors.initials: M
authors.lastname: Alikani
authors.forename: A
authors.initials: A
authors.lastname: Adler
authors.forename: B R
authors.initials: BR
authors.lastname: Gilbert
authors.forename: G D
authors.initials: GD
authors.lastname: Palermo
authors.forename: Z
authors.initials: Z
authors.lastname: Rosenwaks
mesh_terms: D003550:Cystic Fibrosis / Q000235:genetics*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008846:Micromanipulation; D009154:Mutation; D011446:Prospective Studies; D013094:Spermatozoa / Q000502:physiology*; D014649:Vas Deferens / Q000002:abnormalities*
chemical_list: C505032:CFTR protein, human; D008565:Membrane Proteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator