Nitric oxide synthase expression in single hippocampal neurons.
The presence of nitric oxide synthase (NOS) in CA1 pyramidal cells of the rat hippocampus was demonstrated by single-cell PCR. NOS-specific primers were used to amplify mRNA isolated from single hippocampal neurons. The sequence of the major amplification-product obtained was identical to that of the constitutively expressed brain-isoform of NOS. These results confirm immunocytochemical data that NOS is present in CA1, and, therefore, nitric oxide could function as a retrograde messenger in long-term potentiation.
Chiang
L W
LW
Department of Neurobiology, Stanford University Medical Center, CA 94305.
Schweizer
F E
FE
Tsien
R W
RW
Schulman
H
H
D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D004790:Enzyme Induction; D006624:Hippocampus / Q000166:cytology* / Q000502:physiology; D017774:Long-Term Potentiation / Q000502:physiology*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D019001:Nitric Oxide Synthase; D018408:Patch-Clamp Techniques; D016133:Polymerase Chain Reaction; D017966:Pyramidal Cells / Q000201:enzymology*; D051381:Rats
D009419:Nerve Tissue Proteins; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases