title: Danazol decreases transcription of estrogen receptor gene in human monocytes.
abstract: 1. Administration of danazol for over one month reduced the levels of estrogen receptor (ER) and its mRNA to approximately 50 and 20%, respectively in monocytes. 2. Danazol did not alter the degradation rate of ER mRNA in monocytes. 3. Danazol decreased the transcription rate of ER gene to approximately 50% in monocytes in a run-on assay. 4. Danazol may release estrogen predominance via the reduction of transcription for ER gene, which leads to the reduction of ER mRNA and ER expressions in monocytes.
authors.affiliation: Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Fujimoto
authors.forename: M
authors.initials: M
authors.lastname: Hori
authors.forename: T
authors.initials: T
authors.lastname: Itoh
authors.forename: S
authors.initials: S
authors.lastname: Ichigo
authors.forename: M
authors.initials: M
authors.lastname: Nishigaki
authors.forename: T
authors.initials: T
authors.lastname: Tamaya
mesh_terms: D000328:Adult; D001483:Base Sequence; D001667:Binding, Competitive / Q000187:drug effects; D015139:Blotting, Southern; D003613:Danazol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012194:RNA-Directed DNA Polymerase; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D011960:Receptors, Estrogen; D012313:RNA; D012194:RNA-Directed DNA Polymerase; D003613:Danazol