IL-10 inhibits the adhesion of leukocytic cells to IL-1-activated human endothelial cells.
Cytokines play an important role in the recruitment of leukocytes from blood to sites of tissue injury and inflammation. Previous studies showed that the pro-inflammatory cytokine, IL-1, induces the adhesion of leukocytes by up-regulating the expression of the adhesion molecules ICAM-1 and VCAM-1 on endothelial cells. IL-10, a pleiotropic mediator, inhibits the production of cytokines by macrophages and down-regulates the antigen-presenting function of macrophages, thereby acting as a suppressor of immune responses. This study was undertaken to evaluate the effect of IL-10 on the adhesion of leukocytic cells to human umbilical vein endothelial cells. IL-10 inhibited the adhesion of a human monocytic cell line (THP-1) and a human lymphoblastic T-cell line (MOLT-4) to IL-1-stimulated endothelial cells. IL-10 also down-regulated the expression of ICAM-1 and VCAM-1 on IL-1-activated endothelial cells. IL-10-treated THP-1 cells adhered less than untreated THP-1 cells to IL-1-activated endothelial cells, whereas direct treatment of MOLT-4 had minimal effect on its adhesion to cytokine-activated endothelial cells. These results suggest that IL-10 counteracts the pro-inflammatory effects of IL-1 and regulates the adhesion of leukocytic cells to endothelial cells.
Krakauer
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Applied Research Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA.
D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics; D002460:Cell Line; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics; D007375:Interleukin-1 / Q000494:pharmacology*; D016753:Interleukin-10 / Q000494:pharmacology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D013601:T-Lymphocytes / Q000473:pathology; D014407:Tumor Cells, Cultured; D014471:Umbilical Veins; D019010:Vascular Cell Adhesion Molecule-1
D015815:Cell Adhesion Molecules; D007375:Interleukin-1; D016169:Lymphocyte Function-Associated Antigen-1; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D016753:Interleukin-10