title: Effects of acetylcholine and adrenaline on axoplasmic transport at different regions of mouse superior cervical ganglion cells in culture.
abstract: Adrenaline and acetylcholine (ACh) were applied locally at three different positions in cultured superior cervical ganglion cells, i.e., cell body, neurite, and growth cone and the effects on the axoplasmic transport were measured with a video-enhanced microscope. Local ACh application to the cell body, neurite, and growth cone caused the same decreasing effect, but the effects of local adrenaline application were different from each other. Local adrenaline application to the cell body and growth cone caused an increase of axoplasmic transport, but local application at the neurite caused no effect. These data may indicate that there was a lack of beta 2 adrenergic receptors in the neurite. Desensitization of axoplasmic transport was also examined in the SCG neurons. Repetitive adrenaline application to the cell body caused desensitization to the stimulus of adrenaline application.
authors.affiliation: Department of Physiology, Yokohama City University, School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kawakami
authors.forename: T
authors.initials: T
authors.lastname: Takenaka
authors.forename: H
authors.initials: H
authors.lastname: Hori
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: T
authors.initials: T
authors.lastname: Kusakabe
mesh_terms: D000109:Acetylcholine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D001370:Axonal Transport / Q000187:drug effects*; D004837:Epinephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018715:Microscopy, Video; D016501:Neurites / Q000187:drug effects; D013268:Stimulation, Chemical; D017783:Superior Cervical Ganglion / Q000166:cytology / Q000187:drug effects / Q000502:physiology*
chemical_list: D000109:Acetylcholine; D004837:Epinephrine