Effects of ginsenoside Rb1 and Rg1 on synaptosomal free calcium level, ATPase and calmodulin in rat hippocampus.
Calcium homeostasis in synaptosomes is altered during aging. The intrasynaptomal free calcium concentration ([Ca2+]i) was determined in 3- and 24-month-old rats treated with or without Rb1 and Rg1. As expected, the [Ca2+]i level increased with age. Treatment with Rbl and Rgl elicited an obvious decrease of [Ca2+]i content, especially in aged rates. In addition, Rb1 and Rgl significantly stimulated the activity of Na+, K+-ATPase while Rbl inhibited the activity of Ca2+, Mg2+-ATPase and calmodulin. In view of the close relationship of [Ca2+]i level with aging, the changes of [Ca2+]i induced by Rbl and Rgl, as shown by our results, might provide an explanation of the mechanisms of their antiaging function.
Liu
M
M
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Zhang
J
J
D000375:Aging / Q000187:drug effects*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D036145:Ginsenosides; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D005894:Panax; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*
D002147:Calmodulin; D004365:Drugs, Chinese Herbal; D036145:Ginsenosides; D012503:Saponins; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; C035054:ginsenoside Rg1; D002118:Calcium