Endotoxin-induced liver injury and changes in the levels of plasma tumor necrosis factor-alpha and interleukin-6 in rabbits.
ELISA was employed to detect changes in plasma TNF-alpha and IL-6 level in rabbits having endotoxin-induced generalized Shwartzman reaction. Liver injury was assessed by the increase of serum ALT and hepatic histopathologic changes. The results showed that plasma TNF-alpha and IL-6 levels increased remarkably 12h after intravenous injection of endotoxin twice at a 24h interval, and these changes corresponded with the degree of liver injury. Coinjection of dexamethasone and endotoxin partly prevented the elevation of TNF-alpha and IL-6 levels and reduced liver injury. These results indicated that TNF-alpha and IL-6 were involved in endotoxin-induced liver injury.
Wu
C
C
Institute of Integrated Traditional Chinese and Western Medicine, Tongji Medical University, Wuhan.
Li
M
M
Chen
C
C
Zhang
M
M
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury; D004731:Endotoxins; D004926:Escherichia coli; D015850:Interleukin-6 / Q000097:blood*; D008099:Liver / Q000473:pathology; D008107:Liver Diseases / Q000097:blood*; D008297:Male; D009336:Necrosis; D011817:Rabbits; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D004731:Endotoxins; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha