Anti platelet property of anti-single-stranded DNA antibodies in type 1 (insulin-dependent) diabetes mellitus.
Nineteen of 50 sera from children with type 1 (insulin-dependent) diabetes mellitus showed anti-platelet reactivity. This reactivity was significantly associated with the presence of proteoglycan cross-reactive anti-ssDNA antibodies. As well as DNA-anti-DNA interaction, increasing salt concentration of the dilution buffer caused a decrease in the binding of positive sera to platelets. Purified ssDNA inhibited also the anti-platelet reactivity of anti-ssDNA positive sera. The addition of purified IgG from anti-ssDNA positive but not from anti-ssDNA negative sera to washed platelets caused an increased collagen-induced platelet aggregation similar to that obtained with the addition of polycationic agents. It may be suggested that ionic interaction between anti-negative charged molecule antibodies (such as anti-ssDNA antibodies) and platelet surface negative charges may be a pathophysiological mechanism contributing to the altered platelet function observed in diabetes.
Triolo
G
G
Department of Internal Medicine, University of Palermo, Italy.
Palumbo
A A
AA
Giardina
E
E
Davi
G
G
Triolo
G
G
D000293:Adolescent; D001323:Autoantibodies / Q000097:blood*; D001792:Blood Platelets / Q000276:immunology*; D002648:Child; D003094:Collagen / Q000494:pharmacology; D004277:DNA, Single-Stranded / Q000276:immunology*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D066298:In Vitro Techniques; D010974:Platelet Aggregation / Q000187:drug effects; D012016:Reference Values
D001323:Autoantibodies; D004277:DNA, Single-Stranded; D007074:Immunoglobulin G; D003094:Collagen