title: The human proteasome subunit HsN3 is located in the inner rings of the complex dimer.
abstract: Subunit HsN3 of the human proteasome is a beta-type subunit homologous to PRE4 from yeast, X1 beta from Xenopus and RN3 from the rat. Using electron microscopy, the binding sites of a monoclonal antibody with specificity for subunit HsN3 have been located in the two juxtaposed inner rings of the human proteasome. Subunit HsN3 was present in two copies, one in each ring, in accordance with our concept of two identical halves making up the complete human proteasome. The subunit is involved in the trypsin-like as well as the peptidylglutamyl-peptide cleavage activities.
authors.affiliation: Diabetes Forschungsinstitut, Düsseldorf, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Kopp
authors.forename: P
authors.initials: P
authors.lastname: Kristensen
authors.forename: K B
authors.initials: KB
authors.lastname: Hendil
authors.forename: A
authors.initials: A
authors.lastname: Johnsen
authors.forename: A
authors.initials: A
authors.lastname: Sobek
authors.forename: B
authors.initials: B
authors.lastname: Dahlmann
mesh_terms: D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure*; D000939:Epitopes; D005260:Female; D006801:Humans; D016253:Microscopy, Immunoelectron; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000276:immunology / Q000648:ultrastructure*; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D046988:Proteasome Endopeptidase Complex; D011487:Protein Conformation; D017421:Sequence Analysis
chemical_list: D000939:Epitopes; D009097:Multienzyme Complexes; D003546:Cysteine Endopeptidases; C108898:PSMB4 protein, human; D046988:Proteasome Endopeptidase Complex