[Flow cytometry identification of CD34-positive cells].
In the present study three clinical applications of flow-cytometric quantification of CD34-positive cells in the field of blood and marrow transplantation are evaluated. By daily determination of CD34-positive cells in the peripheral blood after mobilization of stem cells with chemotherapy or growth-factors it is possible to set the optimal time for collection of the transplant material. Thereafter the yield of CD34-positive cells within the harvested material is determined. Finally, the efficacy of positive CD34 selection using a biotin-avidin column is evaluated. Initial experience is presented here.
Tichelli
A
A
Departement Zentrallaboratorium, Kantonsspital Basel.
Kralj
A
A
Huxol
H
H
Schönenberger
A
A
Rabaglio
M
M
Borel
J
J
Müller
R
R
Bargetzi
M
M
Gratwohl
A
A
Signer
E
E
D015703:Antigens, CD / Q000302:isolation & purification*; D018952:Antigens, CD34; D001773:Blood Cells / Q000276:immunology / Q000637:transplantation; D016026:Bone Marrow Transplantation; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans
D015703:Antigens, CD; D018952:Antigens, CD34