title: Down-regulation but not phosphorylation of stathmin is associated with induction of HL60 cell growth arrest and differentiation by physiological agents.
abstract: Stathmin is a cytosolic phosphoprotein that has an important but, as yet, undefined role in cell proliferation and differentiation. Induction of growth arrest and differentiation of HL60 cells to monocytes by phorbol 12-myristate 13-acetate is associated with rapid phosphorylation of the protein. Stathmin phosphorylation was not seen when HL60 cells were induced to differentiate to monocytes, by 1 alpha, 25-dihydroxyvitamin D3, and to neutrophils, by all-trans retinoic acid and granulocyte colony stimulating factor. In all the above instances, stathmin expression was down-regulated. Thus, increased stathmin phosphorylation is not required for cell growth arrest or differentiation or down-regulation of stathmin expression.
authors.affiliation: Department of Immunology, University of Birmingham Medical School, Edgbaston, UK.
authors.forename: W E
authors.initials: WE
authors.lastname: Johnson
authors.forename: N A
authors.initials: NA
authors.lastname: Jones
authors.forename: D C
authors.initials: DC
authors.lastname: Rowlands
authors.forename: A
authors.initials: A
authors.lastname: Williams
authors.forename: S S
authors.initials: SS
authors.lastname: Guest
authors.forename: G
authors.initials: G
authors.lastname: Brown
mesh_terms: D015153:Blotting, Western; D002117:Calcitriol / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D008868:Microtubule Proteins; D009000:Monocytes / Q000166:cytology*; D009504:Neutrophils / Q000166:cytology*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D050777:Stathmin; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D008868:Microtubule Proteins; D010750:Phosphoproteins; C493995:STMN1 protein, human; D050777:Stathmin; D016179:Granulocyte Colony-Stimulating Factor; D002117:Calcitriol; D013755:Tetradecanoylphorbol Acetate