High-frequency granuloid colony-forming ability of G-CSF receptor possessing CD34 antigen positive human umbilical cord blood hematopoietic progenitors.
The presence of granulocyte colony-stimulating factor receptor (G-CSFR) in CD34+ hematopoietic progenitors is demonstrated by flow cytometry. Cord blood mononuclear cells (MNCs) contain about 1.09 +/- 0.2% CD34+ cells, 0.32 +/- 0.1% CD34+/G-CSFR+ (G-CSFR+) cells, and 0.77 +/- 0.1% CD34+/G-CSFR- (G-CSFR-) cells. The colony-forming ability of G-CSFR+ cells in the presence of G-CSF, granulocyte-macrophage CSF (GM-CSF), interleukin-3 (IL-3), and erythropoietin (Epo) was higher than that of G-CSFR- cells (29.5 vs. 9.8%; p < 0.01). In the fraction of G-CSFR+ cells, the most frequently formed colony type was CFU-G/GM, while burst-forming unit-erythroid (BFU-E) or colony-forming unit-macrophage (CFU-M) were rare. On the other hand, the incidence of BFU-E and CFU-G/GM was similar in the fraction of G-CSFR- cells. This indicates that most granuloid colonies of CD34+ cells were derived from G-CSFR+ cells. These results suggest a lineage commitment for the vast majority of G-CSFR+ hematopoietic progenitors.
Shimoda
K
K
First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Okamura
S
S
Harada
N
N
Kubota
A
A
Iwasaki
H
H
Ohno
Y
Y
Niho
Y
Y
D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D002454:Cell Differentiation; D003114:Colony-Forming Units Assay; D005260:Female; D005434:Flow Cytometry; D006410:Hematopoiesis; D006801:Humans; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000032:analysis*; D013234:Stem Cells / Q000166:cytology*; D014470:Umbilical Cord / Q000166:cytology*
D015703:Antigens, CD; D018952:Antigens, CD34; D016188:Receptors, Granulocyte Colony-Stimulating Factor