title: Effect of the Steel gene product on melanogenesis in avian neural crest cell cultures.
abstract: Mutations at the Steel (Sl) and dominant white spotting (W) loci affect three embryonic lineages: primordial germ cells, hemopoietic stem cells and neural-crest-derived melanocytes. The gene products of these loci are a peptide growth factor, called here stem cell factor (SCF), and its tyrosine kinase receptor, the proto-oncogene c-kit. We have studied how chicken recombinant SCF affects the development of melanocytes from quail neural crest cells in secondary culture under defined conditions. We observed that the total number of neural crest cells, of melanocytes and of their precursors was higher in the presence than in the absence of SCF. Labelling with bromodeoxyuridine showed that SCF had a modest and transient mitogenic effect on the neural crest population. SCF also enhanced the differentiation rate of melanocyte precursors, recognized by the "melanocyte early marker" monoclonal antibody (MelEM MAb), and of melanocytes, since the proportion of both subpopulations significantly increased in the presence of SCF. Finally, SCF increased the survival of the neural crest population since in its presence the total number of cells remained stable while it gradually declined in control cultures. Our results support the notion that SCF sustains the survival of the neural crest population and stimulates the rate of the melanogenic differentiation process.
authors.affiliation: Institut d'Embryologie Cellulaire et Moléculaire, Centre National de la Recherche Scientifique et du Collège de France, Nogent-sur-Marne.
authors.forename: R
authors.initials: R
authors.lastname: Lahav
authors.forename: L
authors.initials: L
authors.lastname: Lecoin
authors.forename: C
authors.initials: C
authors.lastname: Ziller
authors.forename: V
authors.initials: V
authors.lastname: Nataf
authors.forename: J F
authors.initials: JF
authors.lastname: Carnahan
authors.forename: F H
authors.initials: FH
authors.lastname: Martin
authors.forename: N M
authors.initials: NM
authors.lastname: Le Douarin
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D016298:Hematopoietic Cell Growth Factors / Q000502:physiology*; D008544:Melanocytes / Q000502:physiology*; D009432:Neural Crest / Q000166:cytology*; D011518:Proto-Oncogene Proteins / Q000502:physiology; D019009:Proto-Oncogene Proteins c-kit; D011784:Quail / Q000196:embryology*; D020794:Receptor Protein-Tyrosine Kinases / Q000502:physiology; D016184:Receptors, Colony-Stimulating Factor / Q000502:physiology; D019089:Stem Cell Factor
chemical_list: D016298:Hematopoietic Cell Growth Factors; D011518:Proto-Oncogene Proteins; D016184:Receptors, Colony-Stimulating Factor; D019089:Stem Cell Factor; D019009:Proto-Oncogene Proteins c-kit; D020794:Receptor Protein-Tyrosine Kinases