Cloning of a cDNA encoding a novel heat-shock protein from Dictyostelium discoideum.
We have cloned, from Dictyostelium discoideum, a cDNA encoding a new heat-shock (HS) protein (Hsp) with a predicted molecular mass of 31,447 Da. Outside of its low molecular mass, this Hsp does not share any similarity with the small Hsp currently identified or with alpha-crystallins. Northern blot analysis indicates that this HS-inducible gene is also developmentally regulated </p>.
de Maria
A C
AC
Departamento de Bioquímica, Universidade de São Paulo, Brazil.
Gomes
S L
SL
Juliania
M H
MH
Mazzarella
R
R
Klein
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D017124:Conserved Sequence; D003459:Crystallins / Q000737:chemistry; D018076:DNA, Complementary; D004023:Dictyostelium / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid
D003459:Crystallins; D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D012333:RNA, Messenger