Dynamic and differential Oct-1 expression during early Xenopus embryogenesis: persistence of Oct-1 protein following down-regulation of the RNA.
As a first step towards the elucidation of the role of the transcription factor Oct-1 in development, we prepared a monoclonal antibody to study the spatio-temporal distribution of Oct-1 protein in vivo. Here we report differential expression of the Oct-1 gene in the Xenopus embryo both at the RNA and the protein level. Transcripts and protein are detected in ectodermal and mesodermal cell lineages, in which the expression exhibits a pattern of progressive spatial restriction in the course of development. The Oct-1 expression as reported here is not correlated with cell density or cell proliferation in the embryo. Our results suggest a role of Oct-1 in the specification and differentiation of neuronal and neural crest cells. In many other cells, the developmental decision to down regulate Oct-1 is delayed, probably due to a high stability of the protein.
Veenstra
G J
GJ
Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Beumer
T L
TL
Peterson-Maduro
J
J
Stegeman
B I
BI
Karg
H A
HA
van der Vliet
P C
PC
Destrée
O H
OH
D000818:Animals; D000911:Antibodies, Monoclonal; D002455:Cell Division; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015536:Down-Regulation; D004475:Ectoderm / Q000166:cytology; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; D012313:RNA / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D029867:Xenopus Proteins; D014982:Xenopus laevis
D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; C494169:POU2F1 protein, Xenopus; D014157:Transcription Factors; D029867:Xenopus Proteins; D012313:RNA