PCR-based analysis of voltage-gated K+ channels in vascular smooth muscle.
Irregularities in K+ currents form the basis of several cardiovascular dysfunctions, among which are arrhythmias and vasospasms. The developmental regulation of voltage-gated K+ channels, however, has been difficult to study. A novel approach was therefore employed to examine these channels in muscle tissue. Primers for a PCR-based analysis were designed using published nucleic acid sequences for voltage-gated K+ channels. Final selection of the primer pairs was based on the homology present in the S4 and H5 transmembrane domains. A specific band was amplified with these primers using RNA isolated from both rat A10 vascular smooth muscle cells and rat heart tissue.
Zahradka
P
P
Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre.
Harris
K D
KD
Triggs-Raine
B
B
Lamontagne
G
G
Leblanc
N
N
D000818:Animals; D001011:Aorta; D001483:Base Sequence; D002460:Cell Line; D004587:Electrophoresis, Agar Gel; D006863:Hydrogen-Ion Concentration; D015636:Magnesium Chloride / Q000378:metabolism; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods*; D015221:Potassium Channels / Q000096:biosynthesis / Q000235:genetics*; D011189:Potassium Chloride / Q000378:metabolism; D012313:RNA / Q000032:analysis; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D015221:Potassium Channels; D015636:Magnesium Chloride; D012313:RNA; D011189:Potassium Chloride