title: Formation of a mutagen, glyoxal, from DNA treated with oxygen free radicals.
abstract: Oxygen free radicals cause extensive chemical changes in DNA, including base and sugar modifications and strand breaks. In the present study we found that a mutagen, glyoxal, is produced by exposure of DNA to an oxygen radical forming system (5 mM FeSO4-EDTA, 37 degrees C, 60 min). It was produced with 17 times higher efficiency than 8-hydroxydeoxyguanosine. Thus it is possible that the formation of glyoxal is one of the major types of damage in DNA exposed to oxygen free radicals.
authors.affiliation: Department of Environmental Oncology, University of Occupational and Environmental Health, Kitakyushu, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Murata-Kamiya
authors.forename: H
authors.initials: H
authors.lastname: Kamiya
authors.forename: N
authors.initials: N
authors.lastname: Iwamoto
authors.forename: H
authors.initials: H
authors.lastname: Kasai
mesh_terms: D000818:Animals; D002417:Cattle; D004247:DNA / Q000187:drug effects* / Q000378:metabolism; D004249:DNA Damage; D004492:Edetic Acid / Q000494:pharmacology; D005296:Ferrous Compounds / Q000494:pharmacology; D005609:Free Radicals / Q000378:metabolism / Q000633:toxicity; D006037:Glyoxal / Q000378:metabolism*; D006151:Guanosine / Q000031:analogs & derivatives / Q000378:metabolism; D009153:Mutagens / Q000378:metabolism*; D009705:Nucleosides / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism / Q000633:toxicity*
chemical_list: D005296:Ferrous Compounds; D005609:Free Radicals; D009153:Mutagens; D009705:Nucleosides; D017382:Reactive Oxygen Species; D006151:Guanosine; C055526:Fe(II)-EDTA; C046215:8-hydroxyguanosine; D006037:Glyoxal; D004247:DNA; D004492:Edetic Acid