title: Evaluation of high-performance liquid chromatography for the separation and determination of arsenic species by on-line high-performance liquid chromatographic-hydride generation-atomic absorption spectrometry.
abstract: An on-line high-performance liquid chromatographic-microwave assisted oxidation-hydride generation-atomic absorption spectrometric (HG-AAS) system (using columns of different kinds) has been developed for the determination of arsenite, arsenate, dimethylarsinate (DMA), monomethylarsonate (MMA), arsenobetaine (AsB) and arsenocholine (AsC) in environmental samples. Ion-pair reversed-phase chromatography using tetrabutylammonium phosphate as the ion-pair reagent and anion-exchange chromatography were evaluated and the analytical performances of each are reported. The detection limits were 97-143 and 10-30 micrograms l-1 for ion-pair reversed-phase and anion-exchange chromatography, respectively. The Hamilton PRP-X 100 anionic column was proposed for the determination of the six species; AsB can be quantitated independently of AsC by taking the difference between readings at pH 6 and pH 10.7. The proposed methods were applied to water samples and sediments and their potential for future application was demonstrated.
authors.affiliation: Departamento de Química Analítica, Facultad de Química, Universidad Complutense de Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Martín
authors.forename: M A
authors.initials: MA
authors.lastname: López-Gonzálvez
authors.forename: M
authors.initials: M
authors.lastname: Gómez
authors.forename: C
authors.initials: C
authors.lastname: Cámara
authors.forename: M A
authors.initials: MA
authors.lastname: Palacios
mesh_terms: D001152:Arsenicals / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D015203:Reproducibility of Results; D013054:Spectrophotometry, Atomic / Q000379:methods*
chemical_list: D001152:Arsenicals