Molecular cloning of a cDNA encoding putative molt-inhibiting hormone from the blue crab, Callinectes sapidus.
A cDNA library was constructed using poly(A+) RNA isolated from eyestalk neural ganglia of the blue crab, Callinectes sapidus. The library was screened using a probe generated by PCR based on the published amino acid sequence of molt-inhibiting hormone from the shore crab, Carcinus maenas. DNA sequence analysis of one positive clone revealed a 339 bp open reading frame encoding a 78-residue putative molt-inhibiting hormone and a 35-residue signal peptide. The deduced amino acid sequence of C. sapidus molt-inhibiting hormone is 79% homologous with that of C. maenas. Northern blot analysis, using a fragment of the cloned molt-inhibiting hormone cDNA as probe, revealed specific hybridization to a single band (approximately 1.4 kb) in RNA extracted from eyestalk but not control tissues.
Lee
K J
KJ
Department of Biology, University of Alabama at Birmingham 35294, USA.
Elton
T S
TS
Bej
A K
AK
Watts
S A
SA
Watson
R D
RD
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003386:Brachyura / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary / Q000378:metabolism; D004441:Ecdysterone / Q000037:antagonists & inhibitors*; D017952:Ganglia, Invertebrate / Q000378:metabolism; D015723:Gene Library; D007447:Invertebrate Hormones / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D007447:Invertebrate Hormones; C012445:molt-inhibiting hormone; D004441:Ecdysterone; D012313:RNA