Human peripheral blood eosinophils express and release interleukin-8.
Eosinophils can synthesize various cytokines. We demonstrate that interleukin-8 (IL-8) mRNA and protein are constitutively expressed by freshly isolated resting human eosinophils. Most of the patients with bronchial asthma or atopic dermatitis show evidence for up-regulated IL-8 protein expression in eosinophils but not in neutrophils, suggesting that an eosinophil-specific cytokine may act in these patients. To investigate whether the intracellular IL-8 can be released, eosinophils were stimulated by different cytokines and platelet-activating factor (PAF). Priming with granulocyte/macrophage-colory-stimulating factor and a subsequent 25-min stimulation with RANTES or PAF resulted in release of IL-8 from highly purified human eosinophils in vitro.
Simon
H U
HU
Swiss Institute of Allergy and Asthma Research (SIAF), Davos.
Yousefi
S
S
Weber
M
M
Simon
D
D
Hölzer
C
C
Hartung
K
K
Blaser
K
K
D001249:Asthma / Q000097:blood / Q000473:pathology; D002478:Cells, Cultured; D018946:Chemokine CCL5; D004804:Eosinophils / Q000187:drug effects / Q000378:metabolism*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000378:metabolism; D008168:Lung / Q000473:pathology; D008222:Lymphokines / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000494:pharmacology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D018946:Chemokine CCL5; D016209:Interleukin-8; D008222:Lymphokines; D010972:Platelet Activating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D013755:Tetradecanoylphorbol Acetate