Identification of the epitope recognized by the human V beta 5-specific monoclonal antibody 42/1C1. Potential implications for disease therapy.
A panel of T-cell clones was generated that was specific for amino acid residues 4-13 of the mycobacterial 65-kd stress protein. All the clones were found to express a member of the V beta 5 family, as defined by PCR. However, the clones could be differentiated on the basis of different staining characteristics with the mAb 42/1C1. This antibody is known to recognize both V beta 5.2 and V beta 5.3, as was the PCR primer pair used in the analysis. Sequencing of the TCRs revealed that those clones which were not stained by 42/1C1 expressed a previously unidentified member of the V beta 5 family. By comparing the sequences of the V beta 5 family members that are recognized by 42/1C1 with those that are not, we were able to identify a probable epitope for the antibody. It is also clear from our data that the TCRs of T cells recognizing identical MHC-peptide combinations, although very similar, may be differentiated by mAbs, thereby posing potential problems in any proposed disease therapy involving treatment with monoclonals.
Henwood
J
J
Department of Rheumatology, University of Birmingham, England.
Goodall
J C
JC
Boylston
A W
AW
Gaston
J S
JS
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001426:Bacterial Proteins / Q000276:immunology; D018834:Chaperonin 60; D018833:Chaperonins / Q000276:immunology; D002999:Clone Cells; D004396:Coloring Agents; D000939:Epitopes / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007167:Immunotherapy; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D018834:Chaperonin 60; D004396:Coloring Agents; D000939:Epitopes; D016693:Receptors, Antigen, T-Cell, alpha-beta; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins