Primary cultures of human hypertrophic prostate tissue in WAJC 404 medium: a study of cell morphology and kinetics.
222 biopsy fragments of human hypertrophic prostate tissue were cultured in WAJC 404 serum-free medium for three weeks. Growth halos were examined after 7, 14 and 21 days of culture by optical and scanning electron microscopy. Colonies formed of two concentric areas showed in the inner halo elementary pseudo-lobular morphological units similar to the prostate structure. The cell morphological patterns of the halo turned out to be four in number. Every cell pattern was defined morphologically, morphometrically and phenotypically. Results indicate that all morphological differences must be attributed to the various phases of cell life, as all cell types were positive to cytokeratin. The nonconstant display of PSAP and PSA showed a moderate tendency to cell differentiation in WAJC 404 medium. Cell kinetics were also studied and revealed a decrease in proliferation after 14 days of culture. Primary cultures from biopsy fragments of human hypertrophic prostate tissue may be used as an experimental model up to the 14th day of culture.
De Angeli
S
S
Laboratorio Colture Cellulari del Centro Trasfusionale, Piazzale dell'Ospedale, Treviso, Italy.
Valenti
F
F
Durante
E
E
Bassani
V
V
Parnigotto
P P
PP
D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D003593:Cytoplasm / Q000648:ultrastructure; D004848:Epithelium / Q000473:pathology; D006801:Humans; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D008940:Mitotic Index; D011467:Prostate / Q000473:pathology*; D011470:Prostatic Hyperplasia / Q000473:pathology*; D013997:Time Factors
D016895:Culture Media, Serum-Free