Persistently enhanced ratio of NMDA and non-NMDA components of rat hippocampal EPSC after block of A1 adenosine receptors at increased [Ca2+]o/[Mg2+]o.
NMDA and non-NMDA receptor-mediated components of excitatory post-synaptic current (EPSC) were studied by in situ whole-cell voltage-clamp recordings in the CA1 field of rat hippocampus. We found that the amplitudes ratio of the NMDA to the non-NMDA components can be strongly increased by blocking A1 adenosine receptors. The necessary conditions for this effect are both, increased Ca2+ and lowered Mg2+ in the external medium. The so achieved increase in the NMDA/non-NMDA ratio of EPSC components is irreversible and no longer depends on the activity of A1 adenosine receptors.
Klishin
A
A
Department of Physico-Chemical Biology of Cellular Membranes, Bogomoletz Institute of Physiology, Kiev, Ukraine.
Lozovaya
N
N
Krishtal
O
O
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D004594:Electrophysiology; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology*; D016202:N-Methylaspartate / Q000378:metabolism* / Q000502:physiology; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D058915:Purinergic P1 Receptor Antagonists; D051381:Rats; D017208:Rats, Wistar; D017470:Receptors, Glutamate / Q000187:drug effects / Q000378:metabolism*; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D013806:Theophylline / Q000031:analogs & derivatives / Q000494:pharmacology
D007473:Ion Channels; D058915:Purinergic P1 Receptor Antagonists; D017470:Receptors, Glutamate; C053907:8-cyclopentyl-1,3-dimethylxanthine; D016202:N-Methylaspartate; D013806:Theophylline; D008274:Magnesium; D002118:Calcium