title: Somatostatin- and substance P-like immunoreactivity in rat neostriatal cultures.
abstract: In the normal adult neostriatum, somatostatin immunoreactive interneurons constitute a few percent of the total neuronal population whereas substance P immunoreactive neurons, which project to the substantia nigra, constitute nearly half of the total. Primary monolayer neostriatal cultures derived from E17 rat brains displayed both somatostatin-like and substance P-like immunoreactivity (SOM-IR and SP-IR). However, the proportions of SOM-IR and SP-IR neurons in vitro were significantly different from those in vivo. At 4 days in vitro (DIV), SOM-IR neurons comprised 19% of all neurons and this percentage increased to 30% at 25 DIV. In contrast, SP-IR neurons were less common than expected at 4 DIV (20%) and declined in percentage to 13% at 27 DIV. These results suggest that survival in target-deprived neostriatal cultures is favored for SOM-IR interneurons.
authors.affiliation: Department of Anatomy and Neurobiology, College of Medicine, University of Tennessee, Memphis 38163.
authors.forename: E
authors.initials: E
authors.lastname: Galarraga
authors.forename: S T
authors.initials: ST
authors.lastname: Kitai
authors.forename: D J
authors.initials: DJ
authors.lastname: Surmeier
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016922:Cellular Senescence; D004622:Embryo, Mammalian; D007150:Immunohistochemistry; D017072:Neostriatum / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D013004:Somatostatin / Q000032:analysis* / Q000378:metabolism; D013373:Substance P / Q000032:analysis* / Q000378:metabolism; D013997:Time Factors
chemical_list: D013373:Substance P; D013004:Somatostatin