title: Localization of NADPH-diaphorase reactivity in the chick and mouse thyroid gland.
abstract: In the present study, nicotinamide adenine dinucleotide hydrogen phosphate-diaphorase (NADPH-d) histochemistry has been used as a marker for nitric oxide synthase (NOS). The colored reaction product, formazan, was localized in neuronal cell bodies, nerve fibers, and vascular endothelium in the thyroid of chick and mouse. In these two animal species, most of the NADPH-d-labeled neuronal cell bodies were found in the thyroid capsule and interfollicular connective tissue while some were associated with blood vessels. Most nerve fibers travelled with blood vessels supplying the thyroid gland, while a few of them were intimately associated with the thyroid follicular cells. Control sections not incubated with beta-NADPH failed to show labeling of the above structures. It is concluded that nitric oxide may play an important role in endocrine secretion by controlling the regional blood flow in the thyroid gland and by directly acting on the thyroid follicular cells.
authors.affiliation: Department of Anatomy, National University of Singapore, Kent Ridge.
authors.forename: M A
authors.initials: MA
authors.lastname: Syed
authors.forename: S K
authors.initials: SK
authors.lastname: Leong
authors.forename: A S
authors.initials: AS
authors.lastname: Chan
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000032:analysis; D000818:Animals; D002645:Chickens; D004730:Endothelium, Vascular / Q000737:chemistry; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009252:NADPH Dehydrogenase / Q000032:analysis*; D009412:Nerve Fibers / Q000737:chemistry; D009474:Neurons / Q000737:chemistry; D019001:Nitric Oxide Synthase; D013961:Thyroid Gland / Q000098:blood supply / Q000737:chemistry* / Q000294:innervation
chemical_list: D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase