Renal carcinogenesis in the Eker rat.
The Eker rat hereditary renal carcinoma is an excellent example of a Mendelian dominant predisposition to a specific cancer in an experimental animal. We recently reported that a germline insertion in the rat homologue of the human tuberous sclerosis (TSC2) gene gives rise to the dominantly inherited cancer in the Eker rat model. The function of the TSC2/Tsc2 gene product (called "tuberine" in the human case) is not yet understood, although it contains a short amino acid sequence homologous to the ras family GTPase-activating proteins (GAP3). In the study, we isolated subtracted cDNA clones having increased expression in Eker renal carcinoma cells, using a modified representational difference analysis method to search for additional genes specifically involved in renal carcinogenesis. Here we identified four genes: the third component of the complement (C3) gene, the fos-related antigen I (fra-1) gene, an unknown gene (designated as being expressed in renal carcinoma: erc) and the calpactine I heavy-chain (Annexin II) gene.
Hino
O
O
Department of Experimental Pathology, Cancer Institute, Tokyo, Japan.
Kobayashi
E
E
Nishizawa
M
M
Kubo
Y
Y
Kobayashi
T
T
Hirayama
Y
Y
Takai
S
S
Kikuchi
Y
Y
Tsuchiya
H
H
Orimoto
K
K
D000818:Animals; D017306:Annexin A2 / Q000235:genetics; D001483:Base Sequence; D002885:Chromosomes, Human, Pair 16; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D005799:Genes, Dominant; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D008297:Male; D000090204:Mesothelin; D008969:Molecular Sequence Data; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D051381:Rats; D011922:Rats, Mutant Strains; D014402:Tuberous Sclerosis / Q000235:genetics; D000099169:Fos-Related Antigen 1
D017306:Annexin A2; D017931:DNA Primers; D018076:DNA, Complementary; D000090204:Mesothelin; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D000099169:Fos-Related Antigen 1; C000715529:Msln protein, rat