title: Synovial tissue response to treatment with Campath-1H.
abstract: OBJECTIVE
  Therapeutic trials in rheumatoid arthritis with the monoclonal antibody Campath-1H have demonstrated recurrent clinical synovitis in some patients, despite profound depletion of circulating lymphocytes. This study was undertaken to examine the cellular infiltrates in synovial tissue at a time of persistent peripheral lymphopenia.
  
  
  METHODS
  Immunohistochemical staining of synovial tissue and peripheral blood lymphocyte phenotyping.
  
  
  RESULTS
  Synovial tissues from 2 patients with recurrent synovitis after Campath-1H therapy contained significant T lymphocytic infiltrates at a time when circulating T lymphocytes were markedly depleted.
  
  
  CONCLUSIONS
  These results demonstrate that peripheral blood analysis may not accurately reflect the synovial tissue response to monoclonal antibody therapy.
authors.affiliation: Brigham and Women's Hospital, Boston, Massachusetts.
authors.forename: E M
authors.initials: EM
authors.lastname: Ruderman
authors.forename: M E
authors.initials: ME
authors.lastname: Weinblatt
authors.forename: L M
authors.initials: LM
authors.lastname: Thurmond
authors.forename: G S
authors.initials: GS
authors.lastname: Pinkus
authors.forename: E M
authors.initials: EM
authors.lastname: Gravallese
mesh_terms: D015703:Antigens, CD / Q000009:adverse effects / Q000032:analysis / Q000627:therapeutic use*; D018941:Antigens, CD19; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D000951:Antigens, Neoplasm; D001172:Arthritis, Rheumatoid / Q000628:therapy; D016175:B-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D017252:CD3 Complex / Q000032:analysis; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D000074301:CD52 Antigen; D006023:Glycoproteins; D006801:Humans; D007150:Immunohistochemistry; D007720:Knee Prosthesis; D008231:Lymphopenia / Q000209:etiology; D016612:Paraffin Embedding; D013583:Synovial Membrane / Q000276:immunology* / Q000473:pathology
chemical_list: D015703:Antigens, CD; D018941:Antigens, CD19; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte; D000951:Antigens, Neoplasm; D017252:CD3 Complex; D000074301:CD52 Antigen; C000613862:CD52 protein, human; D006023:Glycoproteins