title: RNase mitochondrial RNA processing cleaves RNA from the rat mitochondrial displacement loop at the origin of heavy-strand DNA replication.
abstract: Ribonuclease mitochondrial RNA processing cleaves RNAs from the mammalian mitochondrial main non-coding regulatory region, called the displacement loop. Our data demonstrate that rat cells contain a site-specific ribonuclease mitochondrial RNA processing activity. We found that this enzyme processes the rat mitochondrial displacement-loop RNA substrate at the level of the conserved sequence block 1, a result which is different from that for mouse. This finding correlates with the in-vivo transcriptional analysis of the rat displacement-loop region. Processing by homologous and heterologous ribonuclease mitochondrial RNA enzymes occurs in the same manner, suggesting a conserved mode of substrate recognition.
authors.affiliation: Centro di Studio sui Mitocondri e Metabolismo Energetico, C. N. R. Bari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Tullo
authors.forename: W
authors.initials: W
authors.lastname: Rossmanith
authors.forename: E M
authors.initials: EM
authors.lastname: Imre
authors.forename: E
authors.initials: E
authors.lastname: Sbisà
authors.forename: C
authors.initials: C
authors.lastname: Saccone
authors.forename: R M
authors.initials: RM
authors.lastname: Karwan
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D004261:DNA Replication; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012323:RNA Processing, Post-Transcriptional; D000077278:RNA, Mitochondrial; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D000077278:RNA, Mitochondrial; D012313:RNA; D012260:Ribonucleases