Biosynthesis of mucin derived from a 60-kDa precursor protein in the human stomach.
We studied the biosynthesis of mucin in the human stomach using an anti-mucin core peptide monoclonal antibody, 3G12. Human stomach mucosa was labeled with [35S]methionine, and chased for 3 h. An approximately 60-kDa subunit of human gastric mucin precursor protein was detected in the intracellular product. Under nonreducing conditions, dimer, trimer, and tetramer mucin precursor protein (120, 180, 240 kDa) were detected. Treatment with tunicamycin or endo-beta-N-acetylglucosaminidase H had no effect on the 60-kDa subunit and its oligomers. Extracellular products contained only the high molecular weight mucin, and the secretion was not affected by tunicamycin. By treatment with monensin or brefeldin A, the mature mucin was not secreted extracellularly. These findings suggested that a 60-kDa subunit of the mucin precursor protein was biosynthesized into mature mucin after oligomerization to tetramers, and that neither the oligomerization nor the intracellular transport of the mucin in the human stomach was associated with N-glycosylation.
Sano
S
S
First Department of Internal Medicine, Kochi Medical School, Japan.
Okazaki
K
K
Yamamoto
Y
Y
Slomiany
A
A
Slomiany
B L
BL
D000911:Antibodies, Monoclonal; D020126:Brefeldin A; D046508:Culture Techniques; D003517:Cyclopentanes / Q000494:pharmacology; D005752:Gastric Mucins / Q000096:biosynthesis*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008970:Molecular Weight; D008985:Monensin / Q000494:pharmacology; D011233:Precipitin Tests; D011498:Protein Precursors / Q000096:biosynthesis*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D014415:Tunicamycin / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D003517:Cyclopentanes; D005752:Gastric Mucins; D011498:Protein Precursors; D011500:Protein Synthesis Inhibitors; D014415:Tunicamycin; D020126:Brefeldin A; D008985:Monensin