Purification and properties of two chitinases from Streptomyces sp. J-13-3.
Two chitinases (Chi-A and Chi-B) purified from Streptomyces sp. J-13-3 had the same molecular weights (31,000) and enzymatic properties (optimum pH and temperature of pH 6.0 and 45 degrees C) but had significantly different isoelectric points (3.9 for Chi-A, 3.5 for Chi-B). Chi-A and -B had identical N-terminal amino acid sequences (ADXAAAWNASSVYTGGGSASYNGHN), similar amino acid compositions, and immunological cross-reactivities. A concomitant decrease of Chi-A and increase of Chi-B was observed in their productions during cultivation.
Okazaki
K
K
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
Kato
F
F
Watanabe
N
N
Yasuda
S
S
Masui
Y
Y
Hayakawa
S
S
D000595:Amino Acid Sequence; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000737:chemistry / Q000378:metabolism*; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D013302:Streptomyces / Q000201:enzymology*
D007527:Isoenzymes; D002686:Chitin; D002688:Chitinases