Serological and structural relationships between Escherichia coli O:98 and Yersinia enterocolitica O:11,23 and O:11,24 lipopolysaccharide O-antigens.
The serologically related lipopolysaccharide O-antigens of Yersinia enterocolitica serotypes 11,23 and 11,24 and of Escherichia coli O:98 were analysed by composition analysis, methylation, and the use of one- and two-dimensional nuclear magnetic resonance methods. They were found to be composed of the same basic linear unbranched polysaccharide of repeating tetrasaccharide units containing 2-acetamido-2-deoxy-D-glucose (D-GlcNAc), 2-acetamido-2-deoxy-D-galacturonic acid (D-GalNAcA), and 2-acetamido-2,6-dideoxy-L-glucose (L-quinovosamine, L-QuiNAc), having the structure -->3)-alpha-L-QuipNAc-(1-->4)-alpha-D-GalpNAcA-(1-->3)-alpha -L-QuipNAc-(1-->3)-beta-D-GlcpNAc-(1-->. The lipopolysaccharide O-antigens of E. coli O:98 and Y. enterocolitica O:11,24 were polymers of the above repeating unit, whereas that of Y. enterocolitica O:11,23 was a linear polymer of the same repeating units in which the alpha-D-GalpNAcA residues were stoichiometrically substituted by acetyl groups at O-3.
Marsden
B J
BJ
Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON.
Bundle
D R
DR
Perry
M B
MB
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology; D012091:Repetitive Sequences, Nucleic Acid; D015008:Yersinia enterocolitica / Q000276:immunology*
D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial