title: Both HLA-B*1301 and B*1302 exist in Asian populations and are associated with different haplotypes.
abstract: A B13 split antigen was newly identified with three alloantisera in Japanese, and two B13 split antigens were found in a Thai family. To confirm the variation of B13 and understand the correspondence between the serologic splits and the published B13 alleles, B*1301 and B*1302, we determined the sequences of genes coding for these B13 splits. The common Japanese B13 allele was found to be B*1301, whereas another split antigen was shown to be coded by B*1302. Two B13 variants identified in a Thai individual corresponded to B*1301 and B*1302. Moreover, 57 B13-positive samples from several ethnic groups were examined using the PCR-SSO method. Differing from previous reports, both B*1301 and B*1302 were found in samples from Asian populations. These two alleles were separately associated with different antigens: HLA-B*1301 exhibited a strong association with A2, Cw10, DR12, and DQ7 antigens, whereas HLA-B*1302 was strongly associated with A30, Cw6, DR7, and DQ2 antigens. In addition, applying the PCR-SSCP method, B*1301 and B*1302 could also be simply distinguished from each other.
authors.affiliation: Japanese Red Cross Central Blood Center, Tokyo.
authors.forename: L
authors.initials: L
authors.lastname: Lin
authors.forename: K
authors.initials: K
authors.lastname: Tokunaga
authors.forename: F
authors.initials: F
authors.lastname: Nakajima
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: K
authors.initials: K
authors.lastname: Kashiwase
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: S
authors.initials: S
authors.lastname: Kuwata
authors.forename: E W
authors.initials: EW
authors.lastname: Sideltseva
authors.forename: T
authors.initials: T
authors.lastname: Akaza
authors.forename: K
authors.initials: K
authors.lastname: Tadokoro
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002681:China; D005828:Genetics, Population; D015235:HLA-B Antigens / Q000235:genetics*; D059908:HLA-B13 Antigen; D006239:Haplotypes; D006801:Humans; D016130:Immunophenotyping; D007564:Japan; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012800:Siberia; D013785:Thailand
chemical_list: D015235:HLA-B Antigens; D059908:HLA-B13 Antigen