HIV-plasma viral load detection by branched DNA signal amplification.
To detect HIV-1 plasma viral load in seropositive patients, we applied a new molecular quantitation technique: branched DNA signal amplification (bDNA). We performed bDNA with 99 sera from HIV-1 seropositive patients undergoing antiretroviral therapy. We compared the results obtained with p24 antigenemia and CD4+ cell count. The bDNA proved quite sensitive and available for routine use in laboratories.
Iuliano
R
R
Department of Biomedicine, University of Pisa.
Forastieri
G
G
Brizzi
M
M
Mazzotta
F
F
Ceccherini-Nelli
L
L
D000911:Antibodies, Monoclonal / Q000276:immunology; D001769:Blood / Q000821:virology*; D018791:CD4 Lymphocyte Count; D015342:DNA Probes / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016655:HIV Core Protein p24 / Q000032:analysis; D006679:HIV Seropositivity / Q000097:blood*; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D015342:DNA Probes; D016655:HIV Core Protein p24; D012367:RNA, Viral