Characterisation of potential regulatory elements within the rat preprotachykinin-A promoter.
The rat preprotachykinin-A (rPPT-A) gene encodes the precursor of several tachykinin neuropeptides including substance P. Previous studies have demonstrated the presence of multiple DNA sequences important for directing expression of the rPPT-A gene in dorsal root ganglion neurons within a region of the promoter spanning nucleotides -865 and -47. In order to identify potential cis acting elements, we have carried out DNase 1 footprinting analysis using a series of constructs containing fragments from this region of the promoter. This study has defined three potential AP-1 complex interactions, two E box binding protein interactions and two dG rich elements, which are potentially bound by complexes related to AP-2 or Sp1 in this region of the promoter.
Mendelson
S C
SC
MRC Brain Metabolism Unit, Royal Edinburgh Hospital, UK.
Quinn
J P
JP
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000737:chemistry; D004247:DNA / Q000032:analysis; D003850:Deoxyribonuclease I; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009710:Nucleotide Mapping; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid / Q000502:physiology*; D013373:Substance P / Q000737:chemistry / Q000378:metabolism; D015320:Tachykinins / Q000235:genetics / Q000378:metabolism*
D011498:Protein Precursors; D015320:Tachykinins; C044206:preprotachykinin; D013373:Substance P; D004247:DNA; D003850:Deoxyribonuclease I