Effect of orpiment (As2S3) on cytochrome P-450, glutathione and lipid peroxide levels of rat liver.
Oral administration of orpiment (50 mg/kg b. wt.) caused significant decreases of cytochrome P-450 (44%) and glutathione levels (47%) of rat liver microsomes. Concomitantly, there was an increase in the lipid peroxidation (78%). Orpiment caused significant decreases in the activities of antioxidant enzymes. The glutathione-S-transferase activity was, however, lowered by 42%. The cytochrome P-450-dependent mixed function oxygenase system was also affected. The results show the hepatotoxic nature of orpiment.
Singh
P
P
Department of Biochemistry, Panjab University, Chandigarh, India.
Sharma
R
R
D000818:Animals; D020261:Arsenic Poisoning; D001152:Arsenicals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism*; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013440:Sulfides / Q000633:toxicity*
D001152:Arsenicals; D008054:Lipid Peroxides; D013440:Sulfides; C045816:arsenic trisulfide; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase; D005978:Glutathione