High D-glucose stimulates the cell cycle from the G1 to the S and M phases, but has no competent effect on the G0 phase, in vascular smooth muscle cells.
The effects of high (28mM) D-glucose (HG) on the cell cycle progression were investigated in rat aorta vascular smooth muscle cells (VSMCs) in primary culture, using an immunocytochemical analysis of cell-cycle-specific nuclear antigens. HG had no effect on the cell cycle of the serum-deprived G0 cells, whereas platelet-derived growth factor (PDGF) stimulated the entry of the G0 cells to the G1 phase without a further progression to the S and M phases. HG, but neither mannitol nor L-glucose, stimulated the progression of the PDGF-pretreated G1 cells to the S and M phases, which was blocked by calphostin-C, a protein kinase C (PKC) blocker. HG did not affect the cytosolic Ca2+ concentration ([Ca2+]i). These data suggest that HG has no competent effect on the G0 cells and acts as a progression growth factor (to stimulate the cell cycle from the G1 to the S/M) in VSMCs through the mechanism, which may be insensitive to [Ca2+]i and mediated by PKC.
Hiroishi
G
G
Division of Molecular Cardiology, Kyushu University, Fukuoka, Japan.
Kobayashi
S
S
Nishimura
J
J
Inomata
H
H
Kanaide
H
H
D000818:Animals; D001011:Aorta / Q000166:cytology / Q000187:drug effects; D000077214:Becaplermin; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D016193:G1 Phase; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D019394:Ki-67 Antigen; D007700:Kinetics; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D008938:Mitosis; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects; D009281:Naphthalenes; D009363:Neoplasm Proteins / Q000032:analysis; D009532:Nickel / Q000494:pharmacology; D009687:Nuclear Proteins / Q000032:analysis; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011083:Polycyclic Compounds / Q000494:pharmacology; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D020574:Proto-Oncogene Proteins c-sis; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D016192:Resting Phase, Cell Cycle; D016196:S Phase; D013237:Stereoisomerism; D013997:Time Factors
D019394:Ki-67 Antigen; D009281:Naphthalenes; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D010982:Platelet-Derived Growth Factor; D011083:Polycyclic Compounds; D018809:Proliferating Cell Nuclear Antigen; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D000077214:Becaplermin; D008353:Mannitol; C022838:nickel chloride; D009532:Nickel; D011493:Protein Kinase C; C058819:calphostin C; D005947:Glucose