Interactions of plasma proteins with a novel polysaccharide surfactant physisorbed to polyethylene.
A polysaccharide surfactant, dextran-[1,6 bis(2-hydroxypropyl-1-amine)hexane]-dextran, (D-H-D) was prepared by reacting dextran (Mw = 8200) with epichlorohydrin followed by reaction with 1,6-hexanediamine. The D-H-D polymer product was characterized by gel permeation chromatography (GPC), and 13C-nuclear magnetic resonance spectroscopy (13C-NMR). D-H-D was physisorbed on polyethylene (PE) from aqueous solution, and the adhesion stability and resistance to protein adsorption was examined under static and dynamic flow conditions, using a modified rotating disk system. Modified surfaces were characterized by attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR), electron spectroscopy for chemical analysis (ESCA) and by water contact angles. Under applied shear stresses of up to 73 dyn cm-2, the adhesion of D-H-D on PE was sufficient to inhibit desorption by water (> 90% D-H-D on PE was retained) and 5% SDS surfactant solution (approximately 83% D-H-D retained), as determined by ATR-FTIR. Under similar shear stress conditions, albumin adsorption on D-H-D modified PE was reduced by over 90%, and protein adsorption from fresh human plasma was reduced by approximately 70% compared with unmodified PE. The results are discussed in terms of interfacial forces, and the suitability of this approach for studying protein-surface interactions and for developing a novel class of protein-resistant biomaterials.
Marchant
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Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106.
Yuan
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Szakalas-Gratzl
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D000327:Adsorption; D001672:Biocompatible Materials; D001798:Blood Proteins / Q000737:chemistry*; D002240:Carbohydrate Sequence; D003911:Dextrans / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D011095:Polyethylenes / Q000737:chemistry*; D011412:Propanolamines / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
D001672:Biocompatible Materials; D001798:Blood Proteins; D003911:Dextrans; D011095:Polyethylenes; D011412:Propanolamines; D013501:Surface-Active Agents; C092345:dextran-(1,6-bis(2-hydroxypropyl-1-amino)hexane)-dextran