[Detection of antigen structures in blood cells in various prepared plasma transfusions].
We investigated the content of antigen-bearing cells and cell fragments in Fresh Frozen Plasma (FFP) from blood centers, in Octaplas (virus-inactivated fresh plasma produced with the solvent/detergent technique by the Octapharma Company) and in MB-plasma (virus-inactivated fresh plasma after photodynamic treatment with methylen blue coming from the German Red Cross in Springe, Lower Saxony). With the aid of an immunoassay (MAIPA-test) these plasmas were tested regarding Rhesus-D-antigen, HLA-class-I- and HLA-class-II-antigens, platelet specific antigens HPA-1a/HPA-1b and granulocyte specific antigens NA1/NA2. In Octaplas (n = 10) we did not find cells or cell fragments and no antigen-bearing blood cell structures. In FFP (n = 28) there were platelet specific antigens in 27 cases (96.4%) and HLA-class-I-antigens in 4 cases (14.3%). In MB-plasma (n = 14) we found platelet specific antigens in all cases, HLA-class-I-antigens in 4 cases (18.6%), HLA-class-II-antigens and granulocyte specific antigens in 1 case (7.1%) and Rhesus-D-antigen in 3 cases (21.4%). Plasma derived from whole blood showed lower levels of cells and antigens than plasma which was produced with the aid of the cell separator.
Barz
D
D
Klinik und Poliklinik für Innere Medizin, Medizinischen Fakultät, Universität Rostock.
D001772:Blood Cell Count; D001773:Blood Cells / Q000276:immunology*; D001781:Blood Component Removal / Q000295:instrumentation; D001793:Blood Preservation / Q000379:methods; D000939:Epitopes / Q000032:analysis; D006801:Humans; D007519:Isoantigens / Q000032:analysis*; D010949:Plasma / Q000276:immunology*; D011786:Quality Control
D000939:Epitopes; D007519:Isoantigens