Molecular analysis of an XY mare with gonadal dysgenesis.
In this study, cytogenetic analysis of an infertile mare revealed a 64, XY karyotype. The XY sex-reversed animal had a female phenotype with gonadal dysgenesis. Using Southern blot analysis, we tested for the presence of two Y-specific genes SRY and ZFY by using DNA isolated from peripheral blood leukocytes. The results showed that at least the DNA-binding domain of the SRY gene was deleted from the Y chromosome of the XY mare but that the ZFY gene was present on this chromosome.
Pailhoux
E
E
INRA, Centre de Recherches de Jouy, Jouy-en-Josas, France.
Cribiu
E P
EP
Parma
P
P
Cotinot
C
C
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005260:Female; D006059:Gonadal Dysgenesis / Q000235:genetics / Q000662:veterinary*; D006734:Horse Diseases / Q000235:genetics*; D006736:Horses; D007621:Karyotyping; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D014998:Y Chromosome / Q000235:genetics
D017931:DNA Primers