Actin-dependent activation of ion conductances in bronchial epithelial cells.
Activation of Cl- and K+ channels is necessary to drive ion secretion in epithelia. There is substantial evidence from previous reports that vesicular transport and exocytosis are involved in the regulation of ion channels. In the present study we examined the role of cytoskeletal elements and components of intracellular vesicle transport on ion channel activation in bronchial epithelial cells. To this end, cells were incubated with a number of different compounds which interact with either microtubules or actin microfilaments, or which interfere with vesicle transport in the Golgi apparatus. The effectiveness of these agents was verified by fluorescence staining of cellular microtubules and actin. The function was examined in 36Cl- efflux studies as well as in whole-cell (WC) patch-clamp and cell-attached studies. The cells were studied under control conditions and after exposure to (in mmol/l) ATP (0.1), forskolin (0.01), histamine (0.01) and hypotonic bath solution (HBS, NaCl 72.5). In untreated control cells, ATP primarily activated a K+ conductance whilst histamine and forskolin induced mainly a Cl- conductance. HBS activated both K+ and Cl- conductances. Incubation of the cells with brefeldin A (up to 100 mumol/l) did not inhibit WC current activation and 36Cl- efflux. Nocodazole (up to 170 mumol/l) reduced the ATP-induced WC current, and mevastatin (up to 100 mumol/l) the cell-swelling-induced WC current. Neither had any effect on the WC current induced by forskolin and histamine. Also 36Cl- efflux induced by HBS, ATP, forskolin and histamine was unaltered by these compounds.(ABSTRACT TRUNCATED AT 250 WORDS)
Hug
T
T
Physiologisches Institut der Albert-Ludwigs-Universität Freiburg, Germany.
Koslowsky
T
T
Ecke
D
D
Greger
R
R
Kunzelmann
K
K
D000199:Actins / Q000502:physiology*; D000255:Adenosine Triphosphate / Q000494:pharmacology; D001980:Bronchi / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D002460:Cell Line; D048429:Cell Size / Q000187:drug effects; D018118:Chloride Channels / Q000187:drug effects / Q000378:metabolism; D005576:Colforsin / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D005456:Fluorescent Dyes; D006801:Humans; D007038:Hypotonic Solutions / Q000494:pharmacology; D007473:Ion Channels / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects / Q000378:metabolism
D000199:Actins; D018118:Chloride Channels; D005456:Fluorescent Dyes; D007038:Hypotonic Solutions; D007473:Ion Channels; D015221:Potassium Channels; D005576:Colforsin; D000255:Adenosine Triphosphate