An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic Golgi fragments.
Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.
Rabouille
C
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Cell Biology Laboratory, Imperial Cancer Research Fund, London, England.
Levine
T P
TP
Peters
J M
JM
Warren
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G
D000251:Adenosine Triphosphatases / Q000502:physiology*; D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology / Q000502:physiology*; D006056:Golgi Apparatus / Q000201:enzymology* / Q000648:ultrastructure; D000077614:Golgi Matrix Proteins; D007425:Intracellular Membranes / Q000502:physiology*; D008565:Membrane Proteins / Q000494:pharmacology; D008854:Microscopy, Electron; D008938:Mitosis / Q000502:physiology*; D008970:Molecular Weight; D052066:N-Ethylmaleimide-Sensitive Proteins; D051381:Rats; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D033921:Vesicular Transport Proteins; D014981:Xenopus
D002352:Carrier Proteins; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D033921:Vesicular Transport Proteins; C091580:vesicular transport factor p115; D000251:Adenosine Triphosphatases; D052066:N-Ethylmaleimide-Sensitive Proteins; C498737:Nsf protein, rat