Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR.
Mismatch amplification mutation assay primers, specific for a unique base substitution in uidA of Escherichia coli O157:H7, was coupled with primers for the Shiga-like toxin I (SLT-I) and SLT-II genes in a multiplex PCR assay. Analysis of 108 bacteria showed that all Escherichia coli serotype O157:H7 strains were identified simultaneously with the SLT types encoded by these strains.
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Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, D.C. 20204.
Payne
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Feng
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D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics / Q000276:immunology; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial; D005819:Genetic Markers; D005966:Glucuronidase / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D019081:O Antigens; D016133:Polymerase Chain Reaction / Q000379:methods*; D011135:Polysaccharides, Bacterial; D012703:Serotyping; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2
D001427:Bacterial Toxins; D017931:DNA Primers; D005819:Genetic Markers; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D005966:Glucuronidase