Anti-hepatitis B virus activity of a mixture of two monoclonal antibodies in an "inhibition in solution" assay.
Two human monoclonal antibodies with anti-hepatitis B activity were investigated separately and as a mixture by means of an "inhibition in solution" assay. With this assay the capacity of anti-HBs antibodies to inhibit the binding of hepatitis B surface antigen (HBsAg) with solid-phase anti-HBs (Ausria II, Abbott Laboratories) was studied. Both HBsAg of different subtypes and purified Dane particles were used. One of the monoclonal antibodies (9H9) was directed against a conformational epitope (anti-"a" like) but induced incomplete inhibition (80% to 90%) of all HBsAg subtypes; the other (4-7B), active against a linear epitope, caused full inhibition of all HBsAg subtypes except one (HBsAg/adw4). In vitro, a mixture of these two monoclonal antibodies was active against HBsAg from liver transplant recipients, including those with suspected variant viruses.
Heijtink
R A
RA
Department of Virology, Erasmus University Rotterdam, The Netherlands.
Kruining
J
J
Weber
Y A
YA
de Man
R A
RA
Schalm
S W
SW
D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology; D000918:Antibody Specificity; D002460:Cell Line; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D006510:Hepatitis B Antibodies / Q000276:immunology* / Q000494:pharmacology; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology; D006801:Humans; D007116:Immunization, Passive; D008099:Liver / Q000821:virology; D016031:Liver Transplantation; D011487:Protein Conformation
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens