Immune responses of sheep to microdissected parts of Haemonchus contortus.
Adult specimens of Haemonchus contortus were microdissected into four body fragments: oesophagus, cuticle of oesophagus, gut and cuticle with adjacent muscle layer. The antigenicity of these different body fragments was analysed in comparison to total (whole) worm extracts with immunoblotting and ELISA using sera of H. contortus-infected sheep. In particular, oesophagus-derived antigens appeared to be specifically recognized and may prove valuable in diagnostic assays.
Takáts
C
C
Department of Parasitology & Zoology, University of Veterinary Science, Budapest, Hungary.
Schallig
H D
HD
Van Leeuwen
M A
MA
Hendrikx
W M
WM
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D004947:Esophagus / Q000276:immunology; D005260:Female; D006188:Haemonchiasis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D006189:Haemonchus / Q000033:anatomy & histology / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000276:immunology / Q000517:prevention & control*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007074:Immunoglobulin G