title: Subsets of CD34+ cells and rapid hematopoietic recovery after peripheral-blood stem-cell transplantation.
abstract: OBJECTIVE
  To study whether there is a relationship between transplanted cell dose and rate of hematopoietic recovery after peripheral-blood stem-cell (PBSC) transplantation, and to obtain an indication whether specific subsets of CD34+ cell populations contribute to rapid recovery of neutrophils or platelets.
  
  
  METHODS
  Based on data from 59 patients, we calculated for each day after PBSC transplantation the dose of CD34+ cells that resulted in rapid recovery of either neutrophils or platelets in the majority (> 70%) of patients. Using dual-color flow cytometry, subsets of peripheral-blood CD34+ cells were quantified and the numbers of CD34+ cells belonging to each of the reinfused subsets correlated with hematopoietic recovery following high-dose chemotherapy.
  
  
  RESULTS
  The calculated threshold values with a high probability of engraftment showed a steep dose-effect relationship between CD34+ cell dose and time to recovery of both neutrophils or platelets. Predominantly CD34+ cells with the phenotype of myeloid precursors were mobilized. A minority of CD34+ cells expressed the erythroid and megakaryocytic lineage-associated antigens and a low but distinct population of CD34+ cells expressed antigens associated with multipotent stem cells. Analysis showed that the number of CD34+CD33- cells (r = -.74, P < .05), as well as the number of CD34+CD41+ cells (r = -.81, P < .005), correlated significantly better with time to neutrophil and platelet recovery, respectively, than with the total number of CD34+ cells (r = -.55 and r = -.56, respectively).
  
  
  CONCLUSIONS
  The numbers of CD34+CD33- cells and CD34+CD41+ cells may help to predict short-term repopulation capacity of PBSCs, especially when relatively low numbers of CD34+ cells per kilogram are reinfused.
authors.affiliation: European Cancer Centre; Department of Medical Oncology, Free University Hospital, Amsterdam, The Netherlands.
authors.forename: M W
authors.initials: MW
authors.lastname: Dercksen
authors.forename: S
authors.initials: S
authors.lastname: Rodenhuis
authors.forename: M K
authors.initials: MK
authors.lastname: Dirkson
authors.forename: W P
authors.initials: WP
authors.lastname: Schaasberg
authors.forename: J W
authors.initials: JW
authors.lastname: Baars
authors.forename: E
authors.initials: E
authors.lastname: van der Wall
authors.forename: I C
authors.initials: IC
authors.lastname: Slaper-Cortenbach
authors.forename: H M
authors.initials: HM
authors.lastname: Pinedo
authors.forename: A E
authors.initials: AE
authors.lastname: Von dem Borne
authors.forename: C E
authors.initials: CE
authors.lastname: van der Schoot
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000378:metabolism*; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D007958:Leukocyte Count; D008875:Middle Aged; D015999:Multivariate Analysis; D009504:Neutrophils; D010976:Platelet Count; D063268:Sialic Acid Binding Ig-like Lectin 3
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic; D000954:Antigens, Surface; C571163:CD33 protein, human; D063268:Sialic Acid Binding Ig-like Lectin 3