title: Superoxide production by neutrophils in children with malignant tumors treated with recombinant human granulocyte colony-stimulating factor.
abstract: BACKGROUND
  Human recombinant granulocyte colony-stimulating factor (rhG-CSF), widely used to combat chemotherapy-induced neutropenia, stimulates both in vivo and in vitro intra- and extra-cellular O2- production in human polymorphonuclear cells (PMNs).
  
  
  METHODS
  Twelve patients with solid tumors or acute lymphoblastic leukemia were treated during induced aplasia with rhG-CSF (5 micrograms/kg/day). Intra- and extracellular O2- production by PMNs isolated from these patients after 5 days of rhG-CSF therapy was assessed following both fMLP and PMA stimulation.
  
  
  RESULTS
  All patients showed a rise in PMN count; administration of rhG-CSF enhanced intra- and extracellular O2- release after fMLP but not after PMA stimulation.
  
  
  CONCLUSIONS
  rhG-CSF potentiates in vivo O2- production by PMNs stimulated with receptor-mediated agonists via G-protein (e.g. fMLP), but not by those stimulated with agonists that bypass receptors via protein kinase C (e.g. PMA).
authors.affiliation: Institute of Pediatrics, University La Sapienza, Rome, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Iacobini
authors.forename: G
authors.initials: G
authors.lastname: Palumbo
authors.forename: S
authors.initials: S
authors.lastname: Bartolozzi
authors.forename: C
authors.initials: C
authors.lastname: Mondaini
authors.forename: M
authors.initials: M
authors.lastname: Castello
authors.forename: A
authors.initials: A
authors.lastname: Clerico
authors.forename: F M
authors.initials: FM
authors.lastname: Perla
authors.forename: B
authors.initials: B
authors.lastname: Werner
authors.forename: G
authors.initials: G
authors.lastname: Digilio
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D006801:Humans; D007223:Infant; D008297:Male; D009369:Neoplasms / Q000097:blood / Q000188:drug therapy*; D009504:Neutrophils / Q000378:metabolism*; D011994:Recombinant Proteins / Q000627:therapeutic use; D013481:Superoxides / Q000097:blood*
chemical_list: D011994:Recombinant Proteins; D013481:Superoxides; D016179:Granulocyte Colony-Stimulating Factor