Resistance against multiple plant viruses in plants mediated by a double stranded-RNA specific ribonuclease.
Many plant viruses have single-stranded RNAs as their genomes. During the course of replication, genomic RNAs and their complementary template RNAs are likely to form double-stranded (ds) RNA at least transiently. To attack replication intermediates of many plant RNA viruses specifically, we introduced a yeast-derived ds-RNA specific RNase gene called pac 1 into plants. The transformed plants showed a decrease in lesion numbers when they were challenged with tomato mosaic virus, and a delay in the appearance of symptoms when inoculated with cucumber mosaic virus or potato virus Y.
Watanabe
Y
Y
Department of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Japan.
Ogawa
T
T
Takahashi
H
H
Ishida
I
I
Takeuchi
Y
Y
Yamamoto
M
M
Okada
Y
Y
D009029:Mosaic Viruses / Q000502:physiology*; D010944:Plants / Q000821:virology*; D030821:Plants, Genetically Modified; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012260:Ribonucleases / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
D012330:RNA, Double-Stranded; D012260:Ribonucleases