title: Localization of integrin subunits alpha 6 and beta 1 during somitogenesis in the long-tailed macaque (M. fascicularis).
abstract: The distribution of integrin subunits alpha 6 and beta 1, and the alpha 6 beta 1 integrin ligand, laminin, was examined during somitogenesis in developmental stages 11, 13, and 16 in the long-tailed macaque, using peroxidase immunocytochemistry. Within differentiating somites in stage 11, alpha 6 expression was observed in the sclerotome, basal surface of dermamyotomal cells adjacent to the basal lamina and on scattered cells throughout the dermamyotome. In further advanced somites in stages 13 and 16, alpha 6 immunoreactivity became restricted to the myotome. alpha 6 was expressed on mesenchymal core cells within the myocele of undifferentiated epithelioid somites and the ventromedial wall of somites commencing differentiation at each stage. beta 1 distribution resembled that of alpha 6 in stage 11 somitic tissue, however, it remained present on myotome and sclerotome cells in the later stages, and was also expressed on dermatomal cells in stage 16. Laminin immunoreactivity, while more intense and prevalent than alpha 6 and beta 1 in each stage examined, occurred on the same somite cell populations as the 2 integrin subunits. These results show a defined distribution of alpha 6 on somitic tissue, and suggest this integrin is involved in somite differentiation. They also support a possible role for alpha 6 in myoblast formation and migration. Overlapping of beta 1 and laminin immunoreactivity with that of alpha 6 further suggests that alpha 6 pairs with beta 1 as a functional heterodimer for laminin in defined somitic regions.
authors.affiliation: California Regional Primate Research Center, University of California, Davis 95616-8542, USA.
authors.forename: C S
authors.initials: CS
authors.lastname: Pow
authors.forename: A G
authors.initials: AG
authors.lastname: Hendrickx
mesh_terms: D000818:Animals; D005314:Embryonic and Fetal Development; D007150:Immunohistochemistry; D039503:Integrin alpha6; D019012:Integrin beta1; D016023:Integrins / Q000032:analysis*; D008251:Macaca / Q000196:embryology* / Q000378:metabolism
chemical_list: D039503:Integrin alpha6; D019012:Integrin beta1; D016023:Integrins