Localization of 12-lipoxygenase mRNA in cultured oligodendrocytes and astrocytes by in situ reverse transcriptase and polymerase chain reaction.
We studied the distribution of 12-lipoxygenase mRNA in glial cells. First, mRNA was detected from cellular extracts by soluble-phase reverse transcriptase-polymerase chain reaction (RT-PCR). Taking into account that cell culture populations could not be 100% homogeneous, we then developed, for the first time, an in situ RT-PCR combined with immunocytochemistry with cell specific markers. Using this procedure we showed that 12-lipoxygenase mRNA was expressed both in mature oligodendrocytes and astrocytes.
Bendani
M K
MK
URA-CNRS 1485, Faculté de Médecine, Limoges, France.
Palluy
O
O
Cook-Moreau
J
J
Beneytout
J L
JL
Rigaud
M
M
Vallat
J M
JM
D000818:Animals; D000831:Animals, Newborn; D001092:Arachidonate 12-Lipoxygenase / Q000096:biosynthesis*; D001253:Astrocytes / Q000201:enzymology*; D001483:Base Sequence; D002478:Cells, Cultured; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009836:Oligodendroglia / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D016707:Tissue Fixation
D012333:RNA, Messenger; D001092:Arachidonate 12-Lipoxygenase; D012194:RNA-Directed DNA Polymerase