CD1-restricted T cell recognition of microbial lipoglycan antigens.
It has long been the paradigm that T cells recognize peptide antigens presented by major histocompatibility complex (MHC) molecules. However, nonpeptide antigens can be presented to T cells by human CD1b molecules, which are not encoded by the MHC. A major class of microbial antigens associated with pathogenicity are lipoglycans. It is shown here that human CD1b presents the defined mycobacterial lipoglycan lipoarabinomannan (LAM) to alpha beta T cell receptor-bearing lymphocytes. Presentation of these lipoglycan antigens required internalization and endosomal acidification. The T cell recognition required mannosides with alpha(1-->2) linkages and a phosphotidylinositol unit. T cells activated by LAM produced interferon gamma and were cytolytic. Thus, an important class of microbial molecules, the lipoglycans, is a part of the universe of foreign antigens recognized by human T cells.
Sieling
P A
PA
Division of Dermatology, University of California at Los Angeles (UCLA) School of Medicine 90095, USA.
Chatterjee
D
D
Porcelli
S A
SA
Prigozy
T I
TI
Mazzaccaro
R J
RJ
Soriano
T
T
Bloom
B R
BR
Brenner
M B
MB
Kronenberg
M
M
Brennan
P J
PJ
D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D018949:Antigens, CD1; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002460:Cell Line; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D007918:Leprosy / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D009166:Mycobacterium leprae / Q000276:immunology; D010716:Phosphatidylinositols / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013045:Species Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D015703:Antigens, CD; D018949:Antigens, CD1; D008070:Lipopolysaccharides; D010716:Phosphatidylinositols; D016693:Receptors, Antigen, T-Cell, alpha-beta; C050016:lipoarabinomannan; C034154:phosphatidylinositol mannoside; D015847:Interleukin-4; D007371:Interferon-gamma