title: Determination of the enantiomers of metoprolol and its major acidic metabolite in human urine by high-performance liquid chromatography with fluorescence detection.
abstract: Enantiomers of metoprolol and its acidic metabolite H 117/04 were determined in human urine by high-performance liquid chromatography (HPLC) with fluorometric detection after chiral derivatization. The carboxyl functional group of the major metabolite was blocked by esterification after solid-phase extraction, which helped to quantitate this compound from interfering substances. The assay method was validated. The recovery of (-)- and (+)-metoprolol from urine was 86.3-90.5%; and the recovery of the (-)- and (+)-acidic metabolite H 117/04 from urine was 74.4-83.9% at different concentrations.
authors.affiliation: Institut National de la Recherche Scientifique, INRS-Santé, Université du Québec, Pointe-Claire, Canada.
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.forename: S F
authors.initials: SF
authors.lastname: Cooper
authors.forename: M
authors.initials: M
authors.lastname: Côté
mesh_terms: D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004951:Esterification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008790:Metoprolol / Q000737:chemistry / Q000652:urine*; D008875:Middle Aged; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
chemical_list: D008790:Metoprolol