title: Mononuclear phagocytic cells in human milk: HLA-DR and Fc gamma R ligand expression.
abstract: The study of the cellular immune components of human milk is essential in the understanding of the role human milk may play in protecting the nursing infant against infection. We have investigated some phenotypic characteristics of breast milk macrophages (BMM) and have compared them to the characteristics of adult peripheral blood monocytes (PBM) by using dual parameter flow microfluorometry. Most BMM expressed the monocyte/macrophage markers Leu-M3 and Leu-M5. The latter marker was present in high density (bright) on BMM, but the density of expression of Leu-M3 was higher on PBM than on BMM [median fluorescence intensity (MFI) 409 +/- 105 versus 203 +/- 106, p = 0.02]. The percentage of BMM (98 +/- 2) that expressed the HLA-DR antigen did not differ significantly from PBM, but the density of expression was higher on BMM (MFI 318 +/- 56 versus 264 +/- 41, p = 0.03). The HLA-DR expression of BMM was further enhanced after incubation with interferon-gamma for 36 h; however, receptor for interleukin-2 could not be induced on BMM by this treatment. The expression of the three classes of Fc gamma R was lower on BMM than on PBM, in percentage (Fc gamma RI 56 +/- 23 versus 79 +/- 17%, p = 0.02), density of expression (Fc gamma RIII MFI 71 +/- 20 versus 153 +/- 73, p = 0.002), or both (Fc gamma RII 74 +/- 22% versus 94 +/- 12%, p = 0.02, and MFI 115 +/- 53 versus 202 +/- 59, p = 0.003).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Newborn Service, George Washington University Hospital, Washington, DC 20037.
authors.forename: R A
authors.initials: RA
authors.lastname: Rivas
authors.forename: A A
authors.initials: AA
authors.lastname: el-Mohandes
authors.forename: I M
authors.initials: IM
authors.lastname: Katona
mesh_terms: D000328:Adult; D003584:Cytological Techniques; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D016167:Integrin alphaXbeta2; D007371:Interferon-gamma / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D008895:Milk, Human / Q000166:cytology* / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D017452:Receptors, IgG / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
chemical_list: D006684:HLA-DR Antigens; D016167:Integrin alphaXbeta2; D017452:Receptors, IgG; D007371:Interferon-gamma