title: HPLC method for the quantitation of cispentacin enantiomers in rat urine.
abstract: The two enantiomers of cispentacin, an antifungal antibiotic, are determined by reversed phase HPLC, after derivatization with Marfey's reagent, in 24 h urine samples collected from rats treated sc and iv with 20 mg/kg cispentacin racemate obtained by synthesis. The application range of the method is 25-250 mg/L for each enantiomer with a precision of 4.0-9.0%. Comparison with an authentic sample of natural origin (-)-cispentacin indicated that (-) enantiomer is excreted unchanged in smaller percentage than (+) enantiomer, which is almost completely eliminated as such.
authors.affiliation: Marion Merrell Dow Research Institute, Lepetit Research Center, Gerenzano, Varese, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Zanol
authors.forename: R
authors.initials: R
authors.lastname: Hermann
authors.forename: A
authors.initials: A
authors.lastname: Bernareggi
authors.forename: M
authors.initials: M
authors.lastname: Borgonovi
authors.forename: E
authors.initials: E
authors.lastname: Taglietti
authors.forename: L F
authors.initials: LF
authors.lastname: Zerilli
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000652:urine; D008297:Male; D051381:Rats; D013237:Stereoisomerism
chemical_list: D000935:Antifungal Agents; D003515:Cycloleucine; C065594:cispentacin