Increased expression of adhesion molecules (CD54, CD29 and CD44) on fibroblasts infected with cytomegalovirus.
The expression of ICAM-1 (CD54), beta 1 integrin (CD29), and CD44 on cytomegalovirus (CMV)-infected human embryonic fibroblasts (HEF) was analyzed by flow cytometry. The expression of these adhesion molecules increased significantly on CMV-infected HEF, on days 2 and 5 after inoculation, compared to uninfected HEF. However, the expression of these adhesion molecules decreased on herpes simplex virus (HSV)-1 and varicella-zoster virus (VZV)-infected HEF. Increased expression was not observed on HEF treated either with inactivated CMV or with supernatant fluid of CMV-infected cells. The addition of anti-cytokine (TNF-alpha, IL-1 beta, or IFN-gamma) antibodies had no effect on the increase of these adhesion molecules. This suggests that the increase in CD54, CD29, and CD44 on CMV-infected cells requires active virus replication and was not mediated by a soluble factor released from CMV-infected cells. Changes in adhesion molecules on CMV-infected fibroblasts may contribute to inflammation induced by CMV infection.
Ito
M
M
Department of Pediatrics, Mie University School of Medicine, Japan.
Watanabe
M
M
Ihara
T
T
Kamiya
H
H
Sakurai
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD / Q000096:biosynthesis*; D002352:Carrier Proteins / Q000096:biosynthesis*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D003470:Culture Media; D016207:Cytokines / Q000276:immunology; D003587:Cytomegalovirus / Q000254:growth & development*; D005347:Fibroblasts / Q000378:metabolism* / Q000382:microbiology; D005434:Flow Cytometry; D018259:Herpesvirus 1, Human / Q000254:growth & development; D014645:Herpesvirus 3, Human / Q000254:growth & development; D006801:Humans; D018960:Hyaluronan Receptors; D019012:Integrin beta1; D016023:Integrins / Q000096:biosynthesis*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D011956:Receptors, Cell Surface / Q000096:biosynthesis*; D016201:Receptors, Lymphocyte Homing / Q000096:biosynthesis*; D014709:Vero Cells; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D002352:Carrier Proteins; D003470:Culture Media; D016207:Cytokines; D018960:Hyaluronan Receptors; D019012:Integrin beta1; D016023:Integrins; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D018799:Intercellular Adhesion Molecule-1