title: The sigma-70 subunit from Escherichia coli C differs from that of E. coli K-12.
abstract: The nucleotide sequence of the rpoD gene (encoding the primary sigma-70 (sigma 70) subunit of RNA polymerase) from Escherichia coli C was determined. This gene differs from that of E. coli K-12 by a 30-bp deletion and five single-bp substitutions, resulting in a sigma 70 subunit with three amino acid (aa) changes and a deletion of ten acidic aa.
authors.affiliation: Department of Microbiology and Immunology, Virginia Commonwealth University, Richmond 23298-0678, USA.
authors.forename: G E
authors.initials: GE
authors.lastname: Christie
authors.forename: S B
authors.initials: SB
authors.lastname: Cale
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D017354:Point Mutation; D017384:Sequence Deletion; D012808:Sigma Factor / Q000235:genetics*; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases