A monoclonal antibody reacting with the cell envelope of spirochaetes isolated from cases of intestinal spirochaetosis in pigs and humans.
A monoclonal antibody (mAb) designed BJL/AC1 was prepared against the cell envelope of an intestinal spirochaete (strain 3295) that was isolated from a pig with intestinal spirochaetosis. The mAb reacted with a band of approximately 29 kDa in cell envelope preparations from 13 porcine and 11 human spirochaetes isolated from cases of intestinal spirochaetosis, but did not react with preparations made from a range of other intestinal spirochaetes. Immunogold labelling demonstrated that the reactive epitope was located on the cell envelope of the strains causing intestinal spirochaetosis. The mAb was used in an indirect immunofluorescence test to detect spirochaetes in the faeces of pigs with experimentally induced intestinal spirochaetosis. The mAb should prove to be a useful reagent for detection and identification of spirochaetes that are specifically associated with intestinal spirochaetosis.
Lee
B J
BJ
School of Veterinary Studies, Murdoch University, Perth, Western Australia.
Hampson
D J
DJ
D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000737:chemistry; D017400:Brachyspira / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure; D002462:Cell Membrane / Q000276:immunology; D005243:Feces / Q000382:microbiology; D006801:Humans; D007410:Intestinal Diseases / Q000382:microbiology*; D051379:Mice; D016253:Microscopy, Immunoelectron; D008970:Molecular Weight; D013142:Spirochaeta / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure; D013143:Spirochaetaceae / Q000276:immunology* / Q000302:isolation & purification / Q000648:ultrastructure; D013145:Spirochaetales Infections / Q000382:microbiology*; D013552:Swine
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial