Studies of radioimmunoassay for measurement of insulin-like growth factor-I (IGF-I) by using anti-IGF-I monoclonal antibody.
Radioimmunoassay system for measurement of insulin-like growth factor-I (IGF-I) was established by using anti-recombinant human IGF-I monoclonal antibody (MAb). This MAb is capable of recognizing not only human but also rat IGF-I. It was thus suggested that this RIA system can quantify IGF-I in human and rat sera. MAb used in this paper was clarified to be an antibody against the common epitope of C-region of human and rat IGF-I. Gly32-Ser33-Ser34 sequence of C-region of IGF-I is discussed to be an antigenic determinant to which this antibody might specifically bind. MAb does not cross-react with proinsulin and insulin as well as anti-IGF-I polyclonal antibody (PAb). And it is general that PAb has almost 2% cross-reactivity with IGF-II. But this MAb did not cross-react with IGF-II. Actually, the value of IGF-I measured by this system was lower than that measured by RIA using PAb.
Tominaga
M
M
Department of Physiology, Osaka City University Medical School, Japan.
Tominaga
K
K
Kinoshita
Y
Y
Terano
Y
Y
Yamamoto
K
K
D000328:Adult; D000818:Animals; D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis* / Q000276:immunology; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007334:Insulin-Like Growth Factor I