Expression of adhesion molecules on human granulocytes after stimulation with Helicobacter pylori membrane proteins: comparison with membrane proteins from other bacteria.
Type B gastritis in its active form is characterized by a dense infiltration of the lamina propria with granulocytes. Since the bacterium Helicobacter pylori does not invade the epithelial barrier, a signaling pathway chemoattractive for granulocytes must exist across this mucosal boarder. One possible mechanism tested was whether granulocytes are directly activated by water-soluble membrane proteins (WSP) from H. pylori. These findings were compared with the effects of WSP from other bacteria (Helicobacter felis, Campylobacter jejuni, Escherichia coli, and Staphylococcus aureus). A unique activation pattern by H. pylori WSP was found. Like all other WSP tested, they induced an upregulation of CD11b but had no influence on CD11c and, most strikingly, CD62L expression. In contrast, E. coli WSP, e.g., not only induce a strong CD11b and CD11c expression but also lead to a loss in surface CD62L. The lack of CD62L shedding conserves rolling of granulocytes along the endothelium, creating a favorable precondition for granulocytes to stick more readily to activated endothelium after H. pylori stimulation via CD11b-CD54 receptor-counterreceptor interaction. This may explain why H. pylori infection is a very strong stimulus for granulocyte infiltration. The active fraction for the induction of CD11b on granulocytes is a heat- and protease-sensitive protein with a molecular mass between 30 and 100 kDa. One activation step involved may be the binding of WSP to CD15 determinants on granulocytes with subsequent induction of CD11b.
Enders
G
G
Institute for Surgical Research, Klinikum Grosshadern, Ludwig-Maximilians University, TU Munich, Germany.
Brooks
W
W
von Jan
N
N
Lehn
N
N
Bayerdörffer
E
E
Hatz
R
R
D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000276:immunology / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D006098:Granulocytes / Q000166:cytology*; D016480:Helicobacter pylori / Q000472:pathogenicity*; D006801:Humans; D066298:In Vitro Techniques; D016167:Integrin alphaXbeta2 / Q000378:metabolism*; D019041:L-Selectin; D016256:Lewis X Antigen / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000378:metabolism*
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D015815:Cell Adhesion Molecules; D016167:Integrin alphaXbeta2; D016256:Lewis X Antigen; D016177:Macrophage-1 Antigen; D019041:L-Selectin