The perinatal presence of antigen (p-azophenylarsonate) or anti-mu antibodies lead to the loss of the recurrent idiotype (CRIA) in A/J mice.
The immune response of A/J mice against p-azophenylarsonate (Ars)-keyhole limpet hemocyanin (KLH) is characterized by the dominance, late in primary and during the secondary, of a recurrent idiotype called CRIA, encoded by a canonical combination of Ig gene segments. In this study, A/J mice were given Ars coupled to deaggregated human gamma globulins (dHGG) within 24 h after delivery. The offsprings from these mice were then exposed as adults to Ars-KLH. These animals developed an unusual immune response. The level of anti-Ars antibodies was nearly normal but a dramatic shift in repertoire was observed: the cross-reactive idiotype which is the hallmark of the anti-Ars response in A/J mice was completely absent. The idiotype could be recovered by injection of anti-idiotypic antibodies alone, with no need of lipopolysaccharide coupling. Therefore the presence of antigen at birth can lead to a strong perturbation of idiotype selection. Similar results were obtained with neonatal treatment using anti-IgM antibodies. After recovery of suppression, A/J mice can mount an anti-arsonate response of normal level but devoid of the dominant idiotype.
Ryelandt
M
M
Laboratoire de Physiologie Animale, Université Libre de Bruxelles, Rhode-saint-Genèse, Belgium.
De Wit
D
D
Baz
A
A
Vansanten
G
G
Denis
O
O
Huetz
F
F
Nisol
F
F
Macedo-Soares
F
F
Barcy
S
S
Brait
M
M
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000915:Antibody Affinity; D007108:Immune Tolerance; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D018655:Lymphocyte Count; D051379:Mice; D008805:Mice, Inbred A; D013234:Stem Cells / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology; D010132:p-Azobenzenearsonate / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; C044203:anti-IgM; D005719:gamma-Globulins; D010132:p-Azobenzenearsonate