Functional link between phosphorylation state of membrane proteins and morphological changes of human erythrocytes.
The Tyr-phosphorylation of the cytoplasmic domain of the major membrane-spanning band 3, rather than the Ser/Thr-phosphorylation of the membrane proteins (spectrin and band 3 itself), might be functionally related to certain morphological changes of human erythrocytes. This view is supported by the following lines of evidence: a) vanadate or its derivative pervanadate (vanadyl hydroperoxide), which markedly increase the Tyr-phosphorylation of band 3 (without practically affecting the Ser/Thr-phosphorylation of spectrin) promotes a crenation of human erythrocytes; b) okadaic acid, which selectively increases the Ser/Thr-phosphorylation of spectrin and other membrane proteins, does not promote any shape change, at least at a level detectable with scanning electron microscopy.
Bordin
L
L
Dipartimento di Chimica Biologica, Centro per lo Studio della Fisiologia dei Mitocondri (CNR), Padova (Italy).
Clari
G
G
Moro
I
I
Dalla Vecchia
F
F
Moret
V
V
D001457:Anion Exchange Protein 1, Erythrocyte / Q000302:isolation & purification / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure*; D004988:Ethers, Cyclic / Q000494:pharmacology; D006801:Humans; D008565:Membrane Proteins / Q000097:blood* / Q000187:drug effects / Q000302:isolation & purification; D008855:Microscopy, Electron, Scanning; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D010750:Phosphoproteins / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D019000:Phosphotyrosine; D013049:Spectrin / Q000302:isolation & purification / Q000378:metabolism; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D014638:Vanadates / Q000494:pharmacology
D001457:Anion Exchange Protein 1, Erythrocyte; D004988:Ethers, Cyclic; D008565:Membrane Proteins; D010750:Phosphoproteins; C076715:pervanadate; D013049:Spectrin; D019319:Okadaic Acid; D019000:Phosphotyrosine; D014638:Vanadates; D014443:Tyrosine; D010749:Phosphoprotein Phosphatases