Oxidative stress and erythrocyte membrane fluidity in patients undergoing regular dialysis.
Oxidative damage due to free radical production is increased in uraemic patients and has been suggested as a possible factor contributing to the anaemia of chronic renal failure (CRF) and the pathogenesis of atherosclerosis. Oxidative stress was assessed in 40 patients with CRF maintained by either haemodialysis (HD) or continuous ambulatory peritoneal dialysis (CAPD) and in 18 healthy controls. Lipid peroxidation (assessed as malondialdehyde, MDA), total glutathione (TG), antioxidant enzyme (glutathione reductase (GSHRx), glutathione peroxidase (GSHPx) and superoxide dismutase (SOD)) activity and antioxidant associated trace metal (selenium, copper, zinc) levels were studied. Erythrocyte membrane fluidity was examined using the fluorescent probe 1,6 diphenyl-1,3,5-hexatriene (DPH). The results indicate increased levels of oxidative stress and altered erythrocyte membrane fluidity in patients treated with CAPD compared with controls and patients treated with HD. Only minor changes were observed in patients treated with HD. Altered free radical activity, oxidative stress and altered erythrocyte membrane fluidity observed in patients with CRF may contribute to the increase in vascular disease in such patients and to the anaemia of CRF.
McGrath
L T
LT
Department of Therapeutics and Pharmacology, School of Clinical Medicine, Queen's University of Belfast, N. Ireland, UK.
Douglas
A F
AF
McClean
E
E
Brown
J H
JH
Doherty
C C
CC
Johnston
G D
GD
Archbold
G P
GP
D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000502:physiology*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy; D008297:Male; D008560:Membrane Fluidity; D008875:Middle Aged; D018384:Oxidative Stress; D010530:Peritoneal Dialysis / Q000009:adverse effects; D006435:Renal Dialysis / Q000009:adverse effects*
D000975:Antioxidants