title: cDNA cloning and gene expression of lebocin, a novel member of antibacterial peptides from the silkworm, Bombyx mori.
abstract: A cDNA encoding lebocin, a novel member of insect antibacterial peptides, was isolated from the fat body cDNA library of Bombyx mori larvae immunized with Escherichia coli. The cDNA was 844 bp long and had an open reading frame (ORF) containing a probable signal peptide (16 amino acids), a putative prosegment (104 amino acids) and a mature peptide (32 amino acids) followed by 27 additional amino acids at its carboxyl-terminus. Northern blot analysis showed that lebocin gene expression was inducible by bacterial injection, occurred tissue-specifically in the fat bodies and continued at least for 48 h post-infection. These results suggest that lebocin has a unique precursor structure and shows typical gene expression pattern as insect antibacterial peptide.
authors.affiliation: Laboratory of Biological Defense, National Institute of Sericultural and Entomological Science, Ibaraki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Chowdhury
authors.forename: K
authors.initials: K
authors.lastname: Taniai
authors.forename: S
authors.initials: S
authors.lastname: Hara
authors.forename: K
authors.initials: K
authors.lastname: Kadono-Okuda
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: S K
authors.initials: SK
authors.lastname: Choi
authors.forename: N C
authors.initials: NC
authors.lastname: Debnath
authors.forename: H K
authors.initials: HK
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology; D001483:Base Sequence; D015152:Blotting, Northern; D012831:Bombyx / Q000737:chemistry; D003001:Cloning, Molecular; D018076:DNA, Complementary; D019476:Insect Proteins; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D011506:Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000494:pharmacology; D012313:RNA / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D000890:Anti-Infective Agents; D018076:DNA, Complementary; D019476:Insect Proteins; D010455:Peptides; D011506:Proteins; C095582:lebocin protein, Bombyx mori; D012313:RNA