Two distinctive BMP-carriers induce zonal chondrogenesis and membranous ossification, respectively; geometrical factors of matrices for cell-differentiation.
A partially purified BMP preparation was combined with a fibrous glass membrane (FGM) or porous particles of hydroxyapatite (PPHAP), and then implanted subcutaneously into the backs of rats. As a control of these new carriers, a conventional carrier of insoluble bone matrix (IBM) was also used. These new geometrically different solid-state carriers induced tissues in quite different manners. FGM/BMP implants induced cartilage formation within the entire inner area of the membrane accompanied by a small amount of bone formation on the surface of the membrane. In contrast, PPHAP/BMP implants induced only bone within the pores of PPHAP without any detectable cartilage formation. Enzyme-linked immunosorbent assay revealed that the type II collagen content in FGM/BMP was six times higher than that in IBM/BMP, while there was no detectable type II collagen in PPHAP/BMP. The results were explained by the geometric properties of the two distinctive carriers.
Kuboki
Y
Y
Department of Biochemistry, School of Dentistry, Hokkaido University, Sapporo, Japan.
Saito
T
T
Murata
M
M
Takita
H
H
Mizuno
M
M
Inoue
M
M
Nagai
N
N
Poole
A R
AR
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001857:Bone Matrix / Q000502:physiology; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D002356:Cartilage / Q000737:chemistry / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D002417:Cattle; D002454:Cell Differentiation; D003094:Collagen / Q000032:analysis; D003238:Connective Tissue / Q000601:surgery; D062109:Dermatologic Surgical Procedures; D017886:Durapatite / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005898:Glass / Q000737:chemistry; D006133:Growth Substances / Q000494:pharmacology*; D008297:Male; D008567:Membranes, Artificial; D010012:Osteogenesis / Q000187:drug effects*; D016062:Porosity; D019736:Prostheses and Implants; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties
D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D006133:Growth Substances; D008567:Membranes, Artificial; D011506:Proteins; D003094:Collagen; D017886:Durapatite