Acute effects of spinal cord injury on the pituitary-testicular hormone axis and Sertoli cell functions: a time course study.
The present study investigated the time course of the onset of the abnormalities in spermatogenesis following spinal cord injury, and their relationship to changes in the pituitary testicular hormonal axis and Sertoli cell function. Spinal cord injury (SCI) was induced in adult male rats by surgical transection of the spinal cord at the level of T9 and L1 vertebrae. Animals were killed 3, 7, and 14 days after the operation. As early as 3 days following SCI, abnormalities in spermatogenesis, including delayed spermiation and vacuolization of the nucleus of spermatids, were noted in both the T9 and L1 animals. By 14 days, other lesions, including phagocytosis of mature spermatids, incomplete cellular associations, and total regression of seminiferous epithelium, became apparent. Concurrently a transient but significant (P < 0.05) suppression of serum follicle-stimulating hormone (FSH) occurred in the T9 animals, and a suppression of serum luteinizing hormone (LH) occurred in both the T9 and the L1 animals 3 days after the surgery. This was accompanied by a suppression of testicular and serum testosterone levels (P < 0.05, P < 0.01, respectively). Most of the hormonal parameters had recovered and were not different from those of sham-operated animals by 14 days (P > 0.10). Northern blot analysis of testicular poly(A)+ RNA revealed a transient but significant reduction in the steady-state level of the 2.7-kilobase (kb) Sertoli cell transferrin mRNA transcript in both the T9 and the L1 animals 3 days after the operation (P < 0.05). On the other hand, the 1.7-kb androgen binding protein (ABP) mRNA remained unaffected during the 2-week study period. The steady-state level of mRNA transcripts for spermatogenic cell-specific hemiferrin and spermatid specific transition protein 2 and protamine 1 also remained unchanged. These results suggest that spinal cord injury will result in a temporary, but profound, effect on the pituitary-testicular hormone axis. These changes may impair certain aspects of Sertoli cell function that could render these cells incapable of supporting normal spermatogenesis. However, the severity of spermatogenic lesions and the disparate responses of the two major Sertoli cell proteins make it unlikely that hormone deficiency is the only mechanism responsible for the impaired spermatogenesis following spinal cord injury.
Huang
H F
HF
Department of Surgery, UMD-New Jersey Medical School, Newark 07103-2757, USA.
Linsenmeyer
T A
TA
Li
M T
MT
Giglio
W
W
Anesetti
R
R
von Hagen
J
J
Ottenweller
J E
JE
Serenas
C
C
Pogach
L
L
D000727:Androgen-Binding Protein / Q000032:analysis; D000818:Animals; D015152:Blotting, Northern; D001835:Body Weight; D004822:Epididymis / Q000737:chemistry / Q000166:cytology; D012739:Gonadal Steroid Hormones / Q000097:blood; D008297:Male; D009929:Organ Size; D010907:Pituitary Hormones / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000502:physiology*; D013091:Spermatogenesis / Q000502:physiology; D013119:Spinal Cord Injuries / Q000503:physiopathology*; D013735:Testicular Hormones / Q000502:physiology*; D013737:Testis / Q000737:chemistry / Q000166:cytology; D013997:Time Factors; D014168:Transferrin / Q000096:biosynthesis
D000727:Androgen-Binding Protein; D012739:Gonadal Steroid Hormones; D010907:Pituitary Hormones; D012333:RNA, Messenger; D013735:Testicular Hormones; D014168:Transferrin