title: High K-ras mutation rates in goblet-cell-type adenocarcinomas of the lungs.
abstract: Adenocarcinomas of the lungs show a variable histology. We have subclassified such lesions into five cell types: hobnail, columnar, polygonal, mixed and goblet cell types, and investigated their relationships with K-ras mutations. Codons 12, 13 and 61 of the K-ras gene in 120 surgically resected pulmonary adenocarcinomas were examined by the mutation-allele-specific amplification method. Point mutations were observed in 10% of the adenocarcinomas limited to K-ras codon 12 and the commonest base substitution (nine cases) was a G to T transversion. Of the five types, goblet cell lesions demonstrated the highest mutation index, which at 100% (6/6) was significantly different from that of all other cell types. No relationship between K-ras mutation and cigarette smoking was observed. From these findings, it appears that development of goblet-cell-type adenocarcinomas of the lung may involve different carcinogenic mechanisms from adenocarcinomas of other subtypes.
authors.affiliation: Department of Pathology, Cancer Institute, Tokyo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Tsuchiya
authors.forename: R
authors.initials: R
authors.lastname: Furuta
authors.forename: N
authors.initials: N
authors.lastname: Wada
authors.forename: K
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: B
authors.initials: B
authors.lastname: Kawabuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Nakamura
authors.forename: H
authors.initials: H
authors.lastname: Sugano
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D001483:Base Sequence; D002454:Cell Differentiation; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D011905:Genes, ras; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D017354:Point Mutation; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics
chemical_list: D017931:DNA Primers; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)