[Antigenic changes in skin after cryopreservation].
It is well accepted impression that antigenic properties of skin are lowered after cryopreservation. In order to prove it, this experiment was done. Fresh guinea pig and cadaveric skin homogenates with appropriate adjuvants were injected hypodermically separately to rabbits once a week. Five week later, blood was withdrawn from rabbits, thus anti-guinea pig skin serum (AGS) and anti-cadaveric skin serum (ACS) were obtained. Homogenates of fresh guinea pig skin (FG) and guinea pig skin stored at -196 degrees C (SG), homogenates of fresh cadaveric skin (FC) and cadaveric skin stored at -196 degrees C (SC) were used as antigens. Microimmunoelectrophoresis and rocket immunoelectrophoresis were performed between related antigens and antibodies. The results of microimmunoelectrophoresis showed that there were four dense and wide precipitated are lines in AGS vs FG and ACS vs FC groups, while only two light and thin precipitated are lines in AGS vs SG and ACS vs SC groups. Rocket immunoelectrophoresis showed that the rocket precipitated area in AGS vs FG and ACS vs FC groups are larger (longer in axis, and broader in width) than in AGS vs SG and ACS vs SC groups. The antigenicity of skin is lowered after being stored at -196 degrees C as compared with that of the fresh one as shown by electrophoresis method.
Jia
X
X
304th Hospital of People's Liberation Army, Beijing.
Zhu
Z
Z
Li
C
C
D000818:Animals; D000941:Antigens / Q000378:metabolism; D015925:Cryopreservation; D000939:Epitopes / Q000032:analysis*; D006168:Guinea Pigs; D006801:Humans; D007106:Immune Sera; D008297:Male; D011817:Rabbits; D012867:Skin / Q000276:immunology*
D000941:Antigens; D000939:Epitopes; D007106:Immune Sera