Location of neurons projecting to the hypophysial stalk--median eminence in ring doves (Streptopelia roseogrisea).
The median eminence/pituitary stalk represents the final common pathway for fibers from neurons that project to the pituitary gland. We have used the lipophilic fluorescent tracer 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) to determine the location of neurons projecting to the median eminence/pituitary stalk in ring doves. The tracer can be precisely applied to fixed tissue, in areas to which it is otherwise difficult to gain access. Following application of DiI to the median eminence/pituitary stalk, labeled neurons were detected in six distinct regions: the ventro-medial hypothalamic nucleus, paraventricular nucleus, supraoptic nucleus, in and ventral to the lateral forebrain bundle, preoptic area, and lateral septum. Labeled fibers branched extensively in the diencephalon, particularly along the third ventricle and in the septal-preoptic area. Sparse fiber labeling occurred caudal to the tuberal hypothalamus, even though these regions were close to the application site of the tracer. Labeled cerebrospinal-fluid-contracting cells were seen in the paraventricular region of the third ventricle. The results indicate that the avian neuronal system that projects to the median eminence and neural lobe occurs in diffuse clusters largely along the midline region of the hypothalamic septal-preoptic area. The paucity of fiber staining caudal to the tuberal hypothalamic region indicates that cells of these regions do not project to the median eminence/pituitary.
Knapp
R
R
Department of Psychology, Barnard College of Columbia University, New York, NY 10027.
Silver
R
R
D000344:Afferent Pathways / Q000166:cytology; D000818:Animals; D001370:Axonal Transport; D001931:Brain Mapping; D002232:Carbocyanines / Q000493:pharmacokinetics; D010856:Columbidae / Q000033:anatomy & histology*; D005260:Female; D005455:Fluorescent Antibody Technique; D007030:Hypothalamo-Hypophyseal System / Q000033:anatomy & histology*; D007032:Hypothalamus, Anterior / Q000166:cytology*; D008297:Male; D008473:Median Eminence / Q000166:cytology; D009474:Neurons / Q000648:ultrastructure*; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology; D010902:Pituitary Gland / Q000166:cytology*; D011301:Preoptic Area / Q000166:cytology; D012686:Septal Nuclei / Q000166:cytology; D013495:Supraoptic Nucleus / Q000166:cytology; D014697:Ventromedial Hypothalamic Nucleus / Q000166:cytology
D002232:Carbocyanines; C024286:3,3'-dioctadecylindocarbocyanine