title: Physiological characterization of a cadmium-resistant mutant in the fission yeast Schizosaccharomyces pombe.
abstract: After treatment of the wild-type strain 975 h+ with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) mutants able to grow on YEP-medium containing 15 mM CdCl2 were isolated. Mutant R17 showed a specific resistance to Cd, but no increased tolerance to Pb(NO3)2, CuSO4 or ZnCl2. No higher content of Cd-binding phytochelatins could be detected, but the mutant accumulated lower levels of Cd than the corresponding wild-type. No change, however, was observed in the accumulation of Cu. Under the influence of Cd, the respiratory activity of mutant R 17 is reduced less than in the wild-type. The mechanism of resistance could be an increased secretion or a decreased uptake of Cd.
authors.affiliation: Institut für Biologie IV (Mikrobiologie), Rheinisch-Westfälische Technische Hochschule Aachen, Worringer Weg, Aachen.
authors.forename: C
authors.initials: C
authors.lastname: Wunderlich
authors.forename: Q
authors.initials: Q
authors.lastname: Zhao
authors.forename: M
authors.initials: M
authors.lastname: Zimmermann
authors.forename: K
authors.initials: K
authors.lastname: Wolf
mesh_terms: D002104:Cadmium / Q000493:pharmacokinetics / Q000633:toxicity*; D003300:Copper / Q000493:pharmacokinetics; D004351:Drug Resistance; D005978:Glutathione / Q000032:analysis; D008667:Metalloproteins / Q000096:biosynthesis; D010101:Oxygen Consumption / Q000187:drug effects; D054811:Phytochelatins; D010940:Plant Proteins / Q000096:biosynthesis; D012568:Schizosaccharomyces / Q000187:drug effects* / Q000502:physiology
chemical_list: D008667:Metalloproteins; D010940:Plant Proteins; D002104:Cadmium; D003300:Copper; D054811:Phytochelatins; D005978:Glutathione