[Detection of mixed lymphoid chimerism after allogeneic bone marrow transplantation: demonstration by interphase cytogenetics in paraffin-embedded tissue].
In bone marrow transplantation (BMT) the detection of residual host lymphoid or haematopoietic cells surviving conditioning therapy is because of its association to graft-versus-host disease, graft-versus-leukemia reaction, and relapse of leukemia a matter of great interest. We studied the occurrence of this mixed lymphoid chimerism (MC) in the formol-fixed lymphatic tissue of lymph nodes and spleen from 21 autopsies after allogeneic sex-mismatched BMT (5 females, 16 males, survival 5 to 1140 days after BMT). In situ hybridisation with biotinylated centromer-specific anti-X- and anti-Y-chromosome probes was performed on pepsin-digested paraffin sections. The number of double X-, single X-, and Y-chromosome bearing cells was analysed microscopically. Because of artefacts only 14 cases remained for valid investigation. MC was detected in 6 cases (5 out of 11 males 5 days to 840 days and 1 out of 3 females 76 days after BMT). MC occurred after whole body irradiation with 10 Gy (n = 5) and 7 Gy (n = 1). In 1 autopsy relapse of leukemia caused host cell infiltration. Cases with MC did not express histological signs of acute or chronic graft-versus-host disease, but 5 out of 8 with complete lymphoid chimerism did. The sensitivity of interphase cytogenetics on paraffin embedded tissue is low.
Friedrich
T
T
Institut für Pathologie, Universität Leipzig.
Ott
G
G
Kalla
J
J
Helbig
W
W
Schwenke
H
H
Kubel
M
M
Pönisch
W
W
Feyer
P
P
Friedrich
A
A
D001344:Autopsy; D016026:Bone Marrow Transplantation / Q000276:immunology* / Q000401:mortality / Q000473:pathology*; D002678:Chimera; D003582:Cytogenetics; D005260:Female; D006650:Histocompatibility Testing; D006652:Histological Techniques; D006801:Humans; D017403:In Situ Hybridization; D008214:Lymphocytes / Q000276:immunology* / Q000473:pathology; D008297:Male; D010232:Paraffin; D012727:Sex Characteristics; D014960:X Chromosome; D014998:Y Chromosome
D010232:Paraffin