title: Immunohistochemical analysis of dichloroacetic acid (DCA)-induced hepatocarcinogenesis in male Fischer (F344) rats.
abstract: We examined the incidence of proliferative lesions, hyperplastic nodules and altered hepatic foci, in male F344 rat liver, to determine their preneoplastic potential during dichloroacetic acid (DCA)-induced hepatocarcinogenesis. Immunohistochemical and image analysis methods were used to detect the expression of 6 histochemical markers of neoplastic cells; p21 ras, p39 c-jun, p55 c-fos, aldehyde dehydrogenase (ALDH), glutathione s-transferase (GST-p), and alpha fetoprotein (AFP) during DCA-induced hepatocarcinogenesis. Our results were consistent with our previous data and suggested that the hyperplastic nodules, rather than altered hepatic foci, is a putative preneoplastic lesion during DCA-induced hepatocarcinogenesis in the male F344 rat.
authors.affiliation: Department of Biological Sciences, Northern Kentucky University, Highland Heights 41099, USA.
authors.forename: R E
authors.initials: RE
authors.lastname: Richmond
authors.forename: J H
authors.initials: JH
authors.lastname: Carter
authors.forename: H W
authors.initials: HW
authors.lastname: Carter
authors.forename: F B
authors.initials: FB
authors.lastname: Daniel
authors.forename: A B
authors.initials: AB
authors.lastname: DeAngelo
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000032:analysis; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D002455:Cell Division; D003999:Dichloroacetic Acid / Q000633:toxicity*; D005982:Glutathione Transferase / Q000032:analysis; D007124:Immunoenzyme Techniques; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D015689:Oncogene Protein p21(ras) / Q000032:analysis; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis; D016755:Proto-Oncogene Proteins c-jun / Q000032:analysis; D051381:Rats; D011916:Rats, Inbred F344; D000509:alpha-Fetoproteins / Q000032:analysis
chemical_list: D014408:Biomarkers, Tumor; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D000509:alpha-Fetoproteins; D003999:Dichloroacetic Acid; D000444:Aldehyde Dehydrogenase; D005982:Glutathione Transferase; D015689:Oncogene Protein p21(ras)