title: An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic Golgi fragments.
abstract: Golgi cisternae regrew in a cell-free system from mitotic Golgi fragments incubated with buffer alone. Pretreatment with NEM or salt washing inhibited regrowth, but this could be restored either by p97, an NSF-like ATPase, or by NSF together with SNAPs and p115, a vesicle docking protein. The morphology of cisternae regrown with p97 and NSF-SNAPs-p115 differed, suggesting that they play distinct roles in rebuilding Golgi cisternae after mitosis.
authors.affiliation: Cell Biology Laboratory, Imperial Cancer Research Fund, London, England.
authors.forename: C
authors.initials: C
authors.lastname: Rabouille
authors.forename: T P
authors.initials: TP
authors.lastname: Levine
authors.forename: J M
authors.initials: JM
authors.lastname: Peters
authors.forename: G
authors.initials: G
authors.lastname: Warren
mesh_terms: D000251:Adenosine Triphosphatases / Q000502:physiology*; D000818:Animals; D002352:Carrier Proteins / Q000494:pharmacology / Q000502:physiology*; D006056:Golgi Apparatus / Q000201:enzymology* / Q000648:ultrastructure; D000077614:Golgi Matrix Proteins; D007425:Intracellular Membranes / Q000502:physiology*; D008565:Membrane Proteins / Q000494:pharmacology; D008854:Microscopy, Electron; D008938:Mitosis / Q000502:physiology*; D008970:Molecular Weight; D052066:N-Ethylmaleimide-Sensitive Proteins; D051381:Rats; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D033921:Vesicular Transport Proteins; D014981:Xenopus
chemical_list: D002352:Carrier Proteins; D000077614:Golgi Matrix Proteins; D008565:Membrane Proteins; D052067:Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins; D033921:Vesicular Transport Proteins; C091580:vesicular transport factor p115; D000251:Adenosine Triphosphatases; D052066:N-Ethylmaleimide-Sensitive Proteins; C498737:Nsf protein, rat