Small-subunit rRNA sequences and whole DNA relatedness concur for the reassignment of Pasteurella piscicida (Snieszko et al.) Janssen and Surgalla to the genus Photobacterium as Photobacterium damsela subsp. piscicida comb. nov.
The taxonomic status of Pasteurella piscicida (strain NCIMB 2058T [T = type strain] and a strain isolated from the environment) was investigated by performing phylogenetic analyses of small-subunit rRNA sequences, DNA-DNA hybridization analyses, and biochemical characterization analyses. The results of the phylogenetic analyses and the levels of DNA-DNA complementarity demonstrated conclusively that Pasteurella piscicida is extremely closely related to Photobacterium damsela ATCC 33539T. Since the two taxa exhibited a level of DNA-DNA relatedness of 80%, they are members of the same species. The high level of DNA relatedness and the presence of specific morphological and biochemical characteristics support the hypothesis that two subspecies should be recognized. On the basis of its phylogenetic position, we concluded that Pasteurella piscicida should be renamed Photobacterium damsela subsp. piscicida comb. nov.
Gauthier
G
G
Centre National de la Recherche Scientifique, Nice, France.
Lafay
B
B
Ruimy
R
R
Breittmayer
V
V
Nicolas
J L
JL
Gauthier
M
M
Christen
R
R
D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D008969:Molecular Sequence Data; D010325:Pasteurella / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D010776:Photobacterium / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D012329:RNA, Bacterial / Q000737:chemistry*; D012335:RNA, Ribosomal / Q000737:chemistry*
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012335:RNA, Ribosomal