A study on lymphocyte activation in maedi-visna virus induced pneumonia.
The stage of activation of bronchoalveolar lavage fluid (BALF) lymphocytes and peripheral blood lymphocytes (PBL) from maedi-visna virus (MVV) infected (n = 7) and control (n = 7) sheep was investigated by assessing four parameters of lymphocyte activation; lymphocyte size and complexity, loss of CD5+ T cells, expression of cell surface interleukin-2 receptor (IL-2R) and expression of DR and DQ MHC Class II molecules. BALF lymphocytes from MVV-infected animals had a significant loss of CD5+ lymphocytes (P < 0.05) and upregulation of DR and DQ MHC Class II molecules compared with controls, consistent with BALF lymphocyte activation. No changes in cell size and complexity or expression of IL-2R were observed. No evidence of PBL activation was detected. These findings suggest an impaired BALF lymphocyte activation during MVV infection.
Begara
I
I
Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, UK.
Lujan
L
L
Hopkins
J
J
Collie
D D
DD
Miller
H R
HR
Watt
N J
NJ
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D018956:CD5 Antigens; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D006683:HLA-DQ Antigens / Q000276:immunology; D006684:HLA-DR Antigens / Q000276:immunology; D016130:Immunophenotyping / Q000662:veterinary; D008213:Lymphocyte Activation; D018655:Lymphocyte Count / Q000662:veterinary; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000276:immunology; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*; D015854:Up-Regulation; D014790:Visna-maedi virus / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018956:CD5 Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D015375:Receptors, Interleukin-2