title: Chemokine expression in murine experimental allergic encephalomyelitis.
abstract: Chemokines are a family of low molecular mass proteins with chemotactic and cell activating activities. Reverse transcription-polymerase chain reaction and Northern hybridization were used to examine their expression during murine experimental allergic encephalomyelitis (EAE), an autoimmune disease used as a model of multiple sclerosis. The mRNAs encoding RANTES, MIP-1 alpha, MIP-1 beta, TCA3 (I-309), IP-10, JE (MCP-1), KC (MGSA/gro), and MARC (MCP-3) were induced in the spinal cord 1-2 days before clinical signs were apparent. SDF, a cDNA predicted to encode a chemokine-like product, was expressed in normal as well as diseased spinal cords. No expression of C10 or MIP-2 was detected. Activated encephalitogenic T cells expressed message for RANTES, MIP-1 alpha, MIP-1 beta, and TCA3. These results define a subset of chemokines that may play an important role in the inflammatory process during murine EAE.
authors.affiliation: ICOS Corporation, Bothell, WA 98021, USA.
authors.forename: R
authors.initials: R
authors.lastname: Godiska
authors.forename: D
authors.initials: D
authors.lastname: Chantry
authors.forename: G N
authors.initials: GN
authors.lastname: Dietsch
authors.forename: P W
authors.initials: PW
authors.lastname: Gray
mesh_terms: D000818:Animals; D001483:Base Sequence; D054402:Chemokine CCL1; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D054410:Chemokine CCL7; D019742:Chemokines, CC; D002630:Chemotactic Factors / Q000235:genetics* / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D005260:Female; D008222:Lymphokines / Q000235:genetics; D019402:Macrophage Inflammatory Proteins; D051379:Mice; D008969:Molecular Sequence Data; D018945:Monocyte Chemoattractant Proteins; D015846:Monokines / Q000235:genetics / Q000378:metabolism; D009185:Myelin Proteins / Q000276:immunology; D018991:Myelin Proteolipid Protein; D010446:Peptide Fragments / Q000276:immunology; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase; D054401:Receptors, CCR8
chemical_list: C065971:CCL1 protein, human; C515450:CCL7 protein, human; C515451:Ccl7 protein, mouse; C489319:Ccr8 protein, mouse; D054402:Chemokine CCL1; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D054410:Chemokine CCL7; D019742:Chemokines, CC; D002630:Chemotactic Factors; D016207:Cytokines; D008222:Lymphokines; D019402:Macrophage Inflammatory Proteins; D018945:Monocyte Chemoattractant Proteins; D015846:Monokines; D009185:Myelin Proteins; D018991:Myelin Proteolipid Protein; D010446:Peptide Fragments; D054401:Receptors, CCR8; C435023:myelin proteolipid protein (139-151); D012194:RNA-Directed DNA Polymerase