Interleukin-4 as a bone regulatory factor: effects on murine osteoblast-like cells.
Bone remodelling is regulated at the local level by an incompletely elucidated cytokine network. In the present study we have determined the effect of interleukin-4 (IL-4), a cytokine produced by T lymphocytes and other cells, on the activity of murine osteoblasts. IL-4 (0.1-10 ng/ml) did not influence the proliferation rate of the osteoblast-like cell line MC3T3, but inhibited the expression of alkaline phosphatase. In long-term cultures supplemented with ascorbic acid and glycerophosphate such an effect was accompanied by a retardation of matrix mineralization. IL-4 also stimulated M-CSF expression by MC3T3 cells, both at the RNA and bioactivity levels. However, no stimulation of IL-1, IL-6, GM-CSF or PGE2 production was observed. An IL-4-induced inhibition of alkaline phosphatase expression and retardation of mineralization was also found in cultures of primary osteoblast-like cells isolated from neonatal mice calvariae. These results suggest that IL-4, probably released by cells within the bone marrow, may locally influence the activity of bone-forming cells.
Riancho
J A
JA
Department of Internal Medicine, Hospital U.M. Valdecilla, University of Cantabria, Santander, Spain.
González-Marcías
J
J
Amado
J A
JA
Olmos
J M
JM
Fernández-Luna
J L
JL
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D016723:Bone Remodeling; D002113:Calcification, Physiologic; D002455:Cell Division; D002460:Cell Line; D015847:Interleukin-4 / Q000494:pharmacology*; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010715:Phosphatidylglycerols / Q000494:pharmacology; D012313:RNA / Q000378:metabolism
D010715:Phosphatidylglycerols; D015847:Interleukin-4; D012313:RNA; D016173:Macrophage Colony-Stimulating Factor; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid