title: The high-conductance channel of porin-less yeast mitochondria.
abstract: Patch-clamp and planar bilayer experiments on porin-less yeast mitochondria have allowed the characterization of a cationic channel activated at matrix-side positive (unphysiological) potentials. In voltage-pulse experiments, inactivation was a faster process than activation and the time constant for inactivation was more steeply dependent on voltage than the one for activation. The channel exhibited various conductance states whose occupancy depended on the applied transmembrane potential. In bilayer experiments, the presence of the pCOx-IV leader peptide induced fast gating in a voltage-dependent manner. A comparison with previously described activities suggests that the pore may coincide with the peptide-sensitive channel (PSC) (Thieffry et al. (1988) EMBO J. 7, 1449-1454) as well as with two other activities (Dihanich et al. (1989) Eur. J. Biochem. 181, 703-708; Tedeschi et al. (1987) J. Membr. Biol. 97, 21-29) assigned to the mitochondrial outer membrane. The possible relationship of this channel to the mitochondrial megachannel is discussed.
authors.affiliation: Centro CNR Fisiologia Mitocondri, Dip. Scienze Biomediche Sperimentali, Padua, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Szabó
authors.forename: G
authors.initials: G
authors.lastname: Báthori
authors.forename: D
authors.initials: D
authors.lastname: Wolff
authors.forename: T
authors.initials: T
authors.lastname: Starc
authors.forename: C
authors.initials: C
authors.lastname: Cola
authors.forename: M
authors.initials: M
authors.lastname: Zoratti
mesh_terms: D007473:Ion Channels / Q000378:metabolism*; D008564:Membrane Potentials; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D018408:Patch-Clamp Techniques; D018272:Porins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D007473:Ion Channels; D018272:Porins; C093986:peptide sensitive channel