Application of the polymerase chain reaction to the detection of African horse sickness viruses.
The development of a coupled reverse transcriptase-polymerase chain reaction assay (RT-PCR) is described for the detection of African horse sickness virus (AHSV) double-stranded RNA. Genome segments 7 and 10 were chosen as target templates for primers selected for use in the RT-PCR. Using these AHSV-specific primers all 9 serotypes were detectable. The sensitivity and specificity of the RT-PCR results were compared to those obtained by competition ELISA.
Zientara
S
S
CNEVA-Laboratoire Central de Recherches Vétérinaires, Maisons-Alfort, France.
Sailleau
C
C
Moulay
S
S
Wade-Evans
A
A
Cruciere
C
C
D000355:African Horse Sickness / Q000821:virology*; D000356:African Horse Sickness Virus / Q000235:genetics / Q000302:isolation & purification*; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006736:Horses; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012194:RNA-Directed DNA Polymerase; D012680:Sensitivity and Specificity; D013154:Spleen / Q000821:virology
D017931:DNA Primers; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase