The genomic structure encoding human initiation factor eIF-5A.
A genomic clone encoding the human eukaryotic initiation factor 5A (eIF-5A) was isolated and its entire nucleotide sequence was determined. The whole eIF-5A coding region is not interrupted by introns. The functional eIF-5A gene is highly homologous to the corresponding complementary DNA. One single 1.4-kb transcript thereof is expressed in human cell lines. Furthermore, we also isolated and sequenced two additional eIF-5A-related sequences which are, by expression and sequence analyses, identified as pseudogenes of the functional eIF-5A. The sequence homology between these pseudogenes and the functional eIF-5A is 71 and 87%.
Koettnitz
K
K
SANDOZ Research Institute, Vienna, Austria.
Kappel
B
B
Baumruker
T
T
Hauber
J
J
Bevec
D
D
D001483:Base Sequence; D003360:Cosmids; D018076:DNA, Complementary; D006367:HeLa Cells; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D016601:RNA-Binding Proteins; D012689:Sequence Homology, Nucleic Acid; D000097574:Eukaryotic Translation Initiation Factor 5A
D018076:DNA, Complementary; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A