Effect of 5-azacytidine and sinefungin on Streptomyces development.
The effect of two DNA-methyltransferase inhibitors, 5-azacytidine (5azaC) and sinefungin (Sf), on the development of Streptomyces antibioticus ETH7451 (Sa) was studied. Pulse labeling experiments and SDS-PAGE analysis of proteins from cells grown in sporulation synthetic medium showed that both inhibitors affect a limited number of systems. Synthesis of the antibiotic rhodomycin was increased in the presence of 5azaC. 5azaC also stimulated the production of actinorhodin in cultures of S. coelicolor A3(2) grown in minimal medium. The analog did not affect the expression of whiB and whiG, two sporulation genes from S. coelicolor A3(2) whose homologues are present in Sa. Overall results indicated that 5azaC and Sf affect specific events associated with differentiation and secondary metabolism in Streptomyces.
Fernandez
M
M
Departamento de Biología Funcional, Universidad de Oviedo, Spain.
Soliveri
J
J
Novella
I S
IS
Yebra
M J
MJ
Barbés
C
C
Sánchez
J
J
D000241:Adenosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D000935:Antifungal Agents / Q000494:pharmacology; D001374:Azacitidine / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D004268:DNA-Binding Proteins; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D005798:Genes, Bacterial; D012808:Sigma Factor; D013171:Spores, Bacterial / Q000187:drug effects; D013302:Streptomyces / Q000187:drug effects / Q000254:growth & development*; D013303:Streptomyces antibioticus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D014157:Transcription Factors / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C081543:whiB protein, Streptomyces; C061314:whiG protein, Streptomyces coelicolor; D000241:Adenosine; D001374:Azacitidine; C006235:sinefungin