Quantification of feline immunodeficiency virus (FIV) proviral DNA in peripheral blood mononuclear cells of cats infected with Japanese strains of FIV.
The polymerase chain reaction (PCR) method was applied for measurement of the proviral DNA copy number of feline immunodeficiency virus (FIV) in peripheral blood mononuclear cells (PBMC) of cats experimentally and naturally infected with FIV. In experimentally infected cats except one cat infected with the Petaluma strain, FIV-specific DNAs were efficiently amplified with the PCR method under the conditions used in this study. In the naturally FIV-infected cats, the specific DNAs were also amplified. We established a quantitative method for measurement of proviral DNA copy number in PBMC from cats infected with TM2-type of FIV strains, and found that the number was variable among the six cats examined, ranging from 10(4.0) to 10(5.7) copies per 10(5) PBMCs. This method can be applicable to cats naturally infected with FIV of TM2-type. Proviral DNA quantitation developed here could be useful as an additional parameter to evaluate the relationships among the proviral load, immune response and development of the clinical symptoms, and to monitor efficacy of antiviral therapy in vivo.
Inoshima
Y
Y
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Tomonaga
K
K
Ikeda
Y
Y
Miyazawa
T
T
Mikami
T
T
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D001483:Base Sequence; D016516:CD4-CD8 Ratio; D002371:Cat Diseases; D002415:Cats; D017931:DNA Primers; D004279:DNA, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D016181:Feline Acquired Immunodeficiency Syndrome / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D016089:Immunodeficiency Virus, Feline / Q000302:isolation & purification*; D008214:Lymphocytes / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011533:Proviruses / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D005719:gamma-Globulins / Q000032:analysis
D000914:Antibodies, Viral; D017931:DNA Primers; D004279:DNA, Viral; D005719:gamma-Globulins