title: [Single channel analysis of aconitine blockade of calcium channels in rat myocardiocytes].
abstract: Ventricular myocardiocytes from neonatal Wistar rats were isolated and cultured. Aconitine, Ca2+ channel blocker verapamil or Ca2+ channel activator BAY K8644 were added to the bath solution separately. Using the cell-attached configuration of the patch clamp technique, the single channel activities of L type Ca2+ channel were recorded before and after addition of all three drugs. The results showed the blocking effect of aconitine (50 micrograms.ml-1) on L type Ca2+ channels. Its mechanism may be relevant to the decrease in both open state probability and the mean open time of Ca2+ channel. The difference was statistically significant compared with control group (P < 0.01). The amplitude of Ba2+ currents, which flow through open L type Ca2+ channel was unchanged.
authors.affiliation: Institute of TCM, Nanjing College of Traditional Chinese Medicine.
authors.forename: L
authors.initials: L
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Ma
authors.forename: B C
authors.initials: BC
authors.lastname: Cai
authors.forename: Y M
authors.initials: YM
authors.lastname: Lu
authors.forename: H
authors.initials: H
authors.lastname: Wu
mesh_terms: D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester / Q000494:pharmacology; D000157:Aconitine / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002478:Cells, Cultured; D009206:Myocardium / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D014700:Verapamil / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D001498:3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; D014700:Verapamil; D000157:Aconitine