Defective functional response to membrane stimuli in lymphocytes from patients with benign prostatic hyperplasia.
Benign prostatic hyperplasia (BPH) is a local disturbance in the prostate that may involve an inflammatory infiltrate predominantly composed of activated lymphocytes and macrophages. The activation and proliferative response of T lymphocytes to different mitogenic signals has been analysed in peripheral blood mononuclear cells (PBMC) from 45 patients with BPH and 55 healthy controls. The PBMC obtained from the patients showed a significant specific impairment in proliferation, CD25 expression and IL-2 production in response to stimulation with lectins (phytohaemagglutinin (PHA), concanavalin A (Con A)), that was not corrected by the addition of IL-2 or of phorbol esters (phorbol myristate acetate (PMA)). Also, the CD28 response was defective in patient PBMC. Activation with anti-CD3 or anti-CD2 MoAbs was normal, but the addition of PMA to these stimuli provoked a significant defective response. Only the use of transmembrane stimuli (PMA and ionomycin) elicited responses similar to those found in the control group. The results indicate that peripheral T lymphocytes from BPH patients show a functional impairment that is mainly explained by an alteration of membrane signals (PHA, CD28) and is distal to protein kinase C (PKC) activation.
Pérez-Blas
M
M
Department of Medicine, Hospital Universitario Principe de Asturias, University of Alcalá de Henares, Madrid, Spain.
Martínez-Martín
B
B
Carballido
J
J
Hontoria
J
J
Salazar
L I
LI
Olivier
C
C
Alvarez-Mon
M
M
D000368:Aged; D018106:CD28 Antigens / Q000096:biosynthesis; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D010703:Phorbol Esters / Q000494:pharmacology; D011470:Prostatic Hyperplasia / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology
D018106:CD28 Antigens; D007376:Interleukin-2; D037102:Lectins; D010703:Phorbol Esters; D015375:Receptors, Interleukin-2