Potassium flux through gramicidin ion channels is augmented in vesicles comprised of plasmenylcholine: correlations between gramicidin conformation and function in chemically distinct host bilayer matrices.
The functional role of distinct phospholipid subclasses and molecular species in modulating gramicidin-mediated K+ flux was characterized through quantification of changes in the fluorescence intensity of ion specific fluorescent probes encapsulated inside vesicles comprised of individual molecular species of plasmenylcholine and phosphatidylcholine. The rate constant of gramicidin-mediated K+ ion flux across bilayers comprised of 1-O-(Z)-hexadec-1'-enyl-2-octadec-9'-enoyl-sn-glycero-3-p hos phocholine (plasmenylcholine) was 18.9 +/- 1.7 s-1, while that present across bilayers comprised of 1-hexadecanoyl-2-octadec-9'-enoyl-sn-glycero-3-phosphocholine (phosphatidylcholine) was 12.3 +/- 1.5 s-1. The observed changes were not due to alterations in the nature of the sn-2 aliphatic chain or the net surface charge present at the membrane interface and were unaltered by the addition of several amphiphilic agents (including charged amphiphiles), suggesting that the observed alterations specifically reflect changes in channel function which result from the covalent alteration of host phospholipid in the proximal portion of the sn-1 aliphatic chain (i.e., phospholipid subclass-specific alterations). Addition of cholesterol to bilayer matrices comprised of plasmenylcholine resulted in dose-dependent attenuation of the rate of gramicidin-mediated K+ flux, but did not alter the rate of gramicidin-mediated K+ flux in membranes comprised of phosphatidylcholine. Gramicidin ion channels experience distinct environments in membranes comprised of phosphatidylcholine and plasmenylcholine host lipids demonstrated by both the different fluorescence anisotropies of endogenous tryptophan residues and the different C=O stretching frequencies of intramonomer carbonyls in gramicidin incorporated into these two choline glycerophospholipid subclasses.(ABSTRACT TRUNCATED AT 250 WORDS)
Chen
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Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Gross
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RW
D002784:Cholesterol; D006096:Gramicidin; D007473:Ion Channels; D007700:Kinetics; D008051:Lipid Bilayers; D008433:Mathematics; D008954:Models, Biological; D008962:Models, Theoretical; D008968:Molecular Conformation; D010713:Phosphatidylcholines; D010955:Plasmalogens; D011188:Potassium / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D013997:Time Factors
D007473:Ion Channels; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D010955:Plasmalogens; C032459:choline plasmalogens; D006096:Gramicidin; D002784:Cholesterol; D011188:Potassium