Partial purification of the rat erythrocyte receptor for the channel-forming toxin aerolysin and reconstitution into planar lipid bilayers.
The cytolytic toxin aerolysin binds to a receptor on the surface of eukaryotic cells. Murine erythrocytes are among the most sensitive to the toxin. Here we describe the detergent solubilization and partial purification of the receptor from rat erythrocytes. We show that it can be successfully incorporated into planar lipid bilayers, greatly decreasing the concentration of aerolysin required to form channels. Exploiting the ability of the receptor to bind aerolysin after SDS electrophoresis and blotting, we obtain evidence that it is a 47 kDa glycoprotein that is sensitive to proteases and N-glycosidase. It may correspond to CHIP28, the water channel of the human erythrocyte.
Gruber
H J
HJ
Institut für Biophysik, Johannes-Kepler-Universität Linz, Linz-Auhof, Austria.
Wilmsen
H U
HU
Cowell
S
S
Schindler
H
H
Buckley
J T
JT
D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D066298:In Vitro Techniques; D007473:Ion Channels / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry; D052899:Pore Forming Cytotoxic Proteins; D051381:Rats; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000302:isolation & purification*
D001427:Bacterial Toxins; D007473:Ion Channels; D008051:Lipid Bilayers; D052899:Pore Forming Cytotoxic Proteins; D011956:Receptors, Cell Surface; C032676:aerolysin