Design and production of a target-specific monoclonal antibody to parvovirus B19 capsid proteins.
Native parvovirus B19 was used as antigen to produce a mouse monoclonal antibody, R92F6, which reacted with B19 VP1 and VP2, neutralised the virus in bone marrow culture, and labelled infected cells in paraffin-embedded tissues from cases of B19-related fetal hydrops. The B19 epitope recognised by R92F6 (amino acids 328-344 from the amino terminal region of B19 VP2) appears to be highly conserved, since these tissue specimens were obtained over a 13 year period from widely spaced locations in the UK. This epitope was synthesised as a peptide (S7b) which was used as antigen to produce a mouse monoclonal antibody, 3H8, which specifically reacted with the B19 capsid proteins VP1 and VP2 in immunofluorescence and immunoblot assays. 3H8 was also capable of labelling formalin-fixed, paraffin-embedded, B19-infected fetal tissue and was shown to be of the same isotype as R92F6 (IgG1). Highly conserved epitopes derived from conserved amino acid sequences are valuable in the diagnosis of infectious disease. If these can be recognised and accurately synthesised, the production of specific mouse monoclonal antibodies may be possible for many human pathogens. Considering the vast amount of sequence data available in the literature, this approach seems to be both feasible and of wide potential.
Kerr
J R
JR
Regional Virus Laboratory, Royal Victoria Hospital, Belfast, Northern Ireland, UK.
O'Neill
H J
HJ
Deleys
R
R
Wright
C
C
Coyle
P V
PV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology*; D000956:Antigens, Viral / Q000032:analysis / Q000276:immunology; D002213:Capsid / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D016731:Erythema Infectiosum / Q000276:immunology / Q000821:virology; D005455:Fluorescent Antibody Technique; D006801:Humans; D015160:Hydrops Fetalis / Q000276:immunology / Q000821:virology; D007124:Immunoenzyme Techniques; D008168:Lung / Q000276:immunology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D016732:Parvovirus B19, Human / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D010446:Peptide Fragments; D014764:Viral Proteins