title: Failure of genotoxic carcinogens to produce tumors in human skin xenografts transplanted to SCID mice.
abstract: Chemical carcinogenesis of human skin was investigated using human skin xenografts (16 full thickness and 48 split thickness skin grafts) transplanted to CB-17-scid (SCID) mice. Topical application of a carcinogen, i.e. 7,12-dimethylbenz[a]anthracene (DMBA), benzo[a]pyrene, methylcholanthrene or N-methyl-N'-nitro-N-nitrosoguanidine, to the human skin xenografts once a week for 25-30 weeks failed to produce skin tumors. Both DMBA application plus UV-B irradiation and alternate applications of the above four carcinogens in combination with UV-B irradiation also failed to produce tumors. All of these treatments induced skin papillomas in skins of host SCID mice. DMBA induced skin papillomas in allogenic CD-1 mouse skin grafts transplanted to SCID mice. These results indicate that susceptibility of human skin to these carcinogenic stimuli is much lower than that of mouse skin.
authors.affiliation: Central Institute for Experimental Animals, Kawasaki, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Urano
authors.forename: Y
authors.initials: Y
authors.lastname: Katakai
authors.forename: Y
authors.initials: Y
authors.lastname: Tokuda
authors.forename: Y
authors.initials: Y
authors.lastname: Ueyama
authors.forename: T
authors.initials: T
authors.lastname: Nomura
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D001711:Biotransformation; D002273:Carcinogens / Q000493:pharmacokinetics / Q000633:toxicity*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D016513:Mice, SCID; D010212:Papilloma / Q000139:chemically induced; D011084:Polycyclic Aromatic Hydrocarbons / Q000493:pharmacokinetics / Q000633:toxicity; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012878:Skin Neoplasms / Q000139:chemically induced*; D016038:Skin Transplantation; D014183:Transplantation, Heterologous
chemical_list: D002273:Carcinogens; D011084:Polycyclic Aromatic Hydrocarbons