title: Analysis of Pneumocystis carinii organism burden, viability and antigens in bronchoalveolar lavage fluid in AIDS patients with pneumocystosis: correlation with disease severity.
abstract: OBJECTIVE
  We examined 96 bronchoalveolar lavage fluid (BALF) specimens from AIDS patients with proven Pneumocystis carinii pneumonia (PCP) in order to compare the relationship of organism burden, viability and antigen expression with disease severity at the time of clinical presentation.
  
  
  METHODS
  Tinctorial analysis of BALF specimens with proven PCP using Diff-Quik, cresyl echt violet and erythrosin B stains to evaluate organism burden and viability. P. carinii antigen examination was performed by Western blot analysis.
  
  
  RESULTS
  P. carinii cluster ratios were more sensitive than cyst counts as an indicator of organism burden, and correlated well with the alveolar-arterial oxygen gradient as a measure of disease severity. Erythrosin B, the vital stain used to measure P. carinii viability, displayed a wide range of values and provided little useful information. Antigens of 35-45 and 95kD, which were specific for P. carinii, were found by immunoblot analysis in BALF cellular fraction of most patients with pneumocystosis. By contrast, antigens of 52 and 66 kD, which were found in both BALF supernatant and cellular fractions of P. carinii patients and controls, most likely represented albumin and immunoglobulin G heavy chain, respectively, of host origin. The 35-45 kD antigen was found in 88% of the BALF specimens and appeared to represent an important marker of P. carinii infection. The 95 kD antigen was detected in 49% of the specimens.
  
  
  CONCLUSIONS
  We conclude that analysis of P. carinii characteristics in BALF specimens of patients with pneumocystis may provide additional information. These data will also be helpful in developing more sensitive assays and in targeting specific P. carinii factors for future investigation.
authors.affiliation: Department of Internal Medicine, University of Cincinnati College of Medicine, Ohio.
authors.forename: A G
authors.initials: AG
authors.lastname: Smulian
authors.forename: M J
authors.initials: MJ
authors.lastname: Linke
authors.forename: M T
authors.initials: MT
authors.lastname: Cushion
authors.forename: R P
authors.initials: RP
authors.lastname: Baughman
authors.forename: P T
authors.initials: PT
authors.lastname: Frame
authors.forename: M N
authors.initials: MN
authors.lastname: Dohn
authors.forename: M L
authors.initials: ML
authors.lastname: White
authors.forename: P D
authors.initials: PD
authors.lastname: Walzer
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology / Q000503:physiopathology*; D000946:Antigens, Fungal / Q000032:analysis*; D015153:Blotting, Western; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D004923:Erythrosine; D015658:HIV Infections / Q000382:microbiology / Q000503:physiopathology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D008970:Molecular Weight; D011010:Pneumocystis / Q000302:isolation & purification*; D011020:Pneumonia, Pneumocystis / Q000382:microbiology / Q000503:physiopathology*; D012016:Reference Values; D013194:Staining and Labeling
chemical_list: D000946:Antigens, Fungal; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D004923:Erythrosine