[Androgen receptors and effects of sex hormones on growth of a human ovarian carcinoma cell line].
Previous study had showed that ovarian carcinoma cell line (HO-8910 cells) contained estrogen receptors (ER) and progesterone receptors (PR). In this experiment, androgen receptors (AR) in these cells were determined by a whole cell binding assay with a synthetic nonmetabolizable androgen-methyltrienolone (3H-R1881). The results showed that there existed high affinity and low capacity, specific binding sites for 3H-R1881 in these cells, the apparent maximum binding capacity and dissociation constant of these binding sites revealed by scathchard analysis being 6.52 fmol/10(6) cells and 2.96 nmol/L respectively, indicating the presence of AR in addition to ER and PR in HO-8910 cells. Furthermore, the effects of sex hormones, i.e., estrodiol, progesterone and testosterone on the growth of HO-8910 cells were also investigated. The results showed that testosterone at 10 nmol/L slightly increased the number of HO-8910 cells, but the increase was not dose-dependent and not statistically significant. Estrodiol and progesterone had no growth-stimulatary or inhibitary effects on these cells.
Lu
J
J
Second Military Medical University, Shanghai.
Zhang
J
J
Zeng
Y
Y
D002455:Cell Division / Q000187:drug effects; D003536:Cystadenocarcinoma / Q000737:chemistry / Q000473:pathology*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D006801:Humans; D010051:Ovarian Neoplasms / Q000737:chemistry / Q000473:pathology*; D011374:Progesterone / Q000494:pharmacology; D011944:Receptors, Androgen / Q000032:analysis*; D013739:Testosterone / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D012739:Gonadal Steroid Hormones; D011944:Receptors, Androgen; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol