Both HLA-B*1301 and B*1302 exist in Asian populations and are associated with different haplotypes.
A B13 split antigen was newly identified with three alloantisera in Japanese, and two B13 split antigens were found in a Thai family. To confirm the variation of B13 and understand the correspondence between the serologic splits and the published B13 alleles, B*1301 and B*1302, we determined the sequences of genes coding for these B13 splits. The common Japanese B13 allele was found to be B*1301, whereas another split antigen was shown to be coded by B*1302. Two B13 variants identified in a Thai individual corresponded to B*1301 and B*1302. Moreover, 57 B13-positive samples from several ethnic groups were examined using the PCR-SSO method. Differing from previous reports, both B*1301 and B*1302 were found in samples from Asian populations. These two alleles were separately associated with different antigens: HLA-B*1301 exhibited a strong association with A2, Cw10, DR12, and DQ7 antigens, whereas HLA-B*1302 was strongly associated with A30, Cw6, DR7, and DQ2 antigens. In addition, applying the PCR-SSCP method, B*1301 and B*1302 could also be simply distinguished from each other.
Lin
L
L
Japanese Red Cross Central Blood Center, Tokyo.
Tokunaga
K
K
Nakajima
F
F
Ishikawa
Y
Y
Kashiwase
K
K
Tanaka
H
H
Kuwata
S
S
Sideltseva
E W
EW
Akaza
T
T
Tadokoro
K
K
D000483:Alleles; D001483:Base Sequence; D002681:China; D005828:Genetics, Population; D015235:HLA-B Antigens / Q000235:genetics*; D059908:HLA-B13 Antigen; D006239:Haplotypes; D006801:Humans; D016130:Immunophenotyping; D007564:Japan; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012800:Siberia; D013785:Thailand
D015235:HLA-B Antigens; D059908:HLA-B13 Antigen