Correlation of cell surface fluorescence with conventional PAP analysis of cells of cytological interest obtained from cervical scrapes.
Archival PAP stained cervical smears were destained and treated with a fluorescent probe for a cell surface enzyme (GB). Cells which exhibited cell surface fluorescence were demonstrated to be cells of cytological interest in the analysis of cervical smears. These cells could be directly related to PAP and reclassified by subsequent restaining with PAP. Fluorescent cell surface technology was shown to be compatible with conventional PAP staining.
Steven
F S
FS
Division of Biochemistry, School of Biological Sciences, University of Manchester, U.K.
Desai
M
M
Davis
J
J
Stedman
Y
Y
McClure
J
J
Eason
P
P
Palcic
B
B
Anderson
G
G
D002584:Cervix Uteri / Q000473:pathology*; D005260:Female; D005453:Fluorescence; D006801:Humans; D013194:Staining and Labeling; D014626:Vaginal Smears