A 200 bp region of the pea ENOD12 promoter is sufficient for nodule-specific and nod factor induced expression.
ENOD12 is one of the first nodulin genes expressed upon inoculation with Rhizobium and also purified Nod factors are able to induce ENOD12 expression. The ENOD12 gene family in pea (Pisum sativum) has two members. A cDNA clone representing PsENOD12A [26] and a PsENOD12B genomic clone [7] have been previously described. The isolation and characterization of a PsENOD12A genomic clone is presented in this paper. By using a Vicia hirsuta-Agrobacterium rhizogenes transformation system it is shown that both genes have a similar expression pattern in transgenic V. hirsuta root nodules. Promoter analyses of both PsENOD12 promoters showed that the 200 bp immediately upstream of the transcription start are sufficient to direct nodule-specific and Nod factor-induced gene expression.
Vijn
I
I
Department of Molecular Biology, Agricultural University, Wageningen, Netherlands.
Christiansen
H
H
Lauridsen
P
P
Kardailsky
I
I
Quandt
H J
HJ
Broer
I
I
Drenth
J
J
Ostergaard Jensen
E
E
van Kammen
A
A
Bisseling
T
T
D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D018532:Pisum sativum / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013559:Symbiosis; D014158:Transcription, Genetic
D017931:DNA Primers; C062870:ENOD12 protein, Pisum sativum; D008070:Lipopolysaccharides; C092736:Nod factor, Rhizobium leguminosarum; D010940:Plant Proteins; D012333:RNA, Messenger