Postselective (directed) mutagenesis of fast-growing strain of Mycobacterium vaccae as a method of creating mutants showing changed phenotypic properties.
Postselective (directed) mutagenesis was used to create mutants of M. vaccae B3805 showing changed abilities for steroid biotransformations. Three morphological classes of such mutants, differing in colony colour: yellow, white and pink, were selected. Some of them can be helpful in genetic studies of steroid biotransformation. Two mutants were also shown to be hosts for foreign DNA.
Sajduda
A
A
Department of Genetics of Microorganisms, University of Lódź, Poland.
Dziadek
J
J
Golańska
E
E
Rumijowska
A
A
Jaworski
A
A
D001711:Biotransformation; D004269:DNA, Bacterial / Q000032:analysis; D007700:Kinetics; D016296:Mutagenesis; D009154:Mutation / Q000502:physiology*; D009161:Mycobacterium / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D012855:Sitosterols / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000378:metabolism; D013256:Steroids / Q000378:metabolism
D004269:DNA, Bacterial; D012855:Sitosterols; D013256:Steroids; C025473:gamma-sitosterol; D013250:Steroid Hydroxylases; C036815:steroid 9-alpha-hydroxylase