title: Recovery and phylogenetic analysis of novel archaeal rRNA sequences from a deep-sea deposit feeder.
abstract: In 1992, two independent reports based on small-subunit rRNA gene (SSU rDNA) cloning revealed the presence of novel Archaea among marine bacterioplankton. Here, we report the presence of further novel Archaea SSU rDNA sequences recovered from the midgut contents of a deep-sea marine holothurian. Phylogenetic analyses show that these abyssal Archaea are a paraphyletic component of a highly divergent clade that also includes some planktonic sequences. Our data confirm that this clade is a deep-branching lineage in the tree of life.
authors.affiliation: Department of Microbiology, University College Galway, Ireland.
authors.forename: J O
authors.initials: JO
authors.lastname: McInerney
authors.forename: M
authors.initials: M
authors.lastname: Wilkinson
authors.forename: J W
authors.initials: JW
authors.lastname: Patching
authors.forename: T M
authors.initials: TM
authors.lastname: Embley
authors.forename: R
authors.initials: R
authors.lastname: Powell
mesh_terms: D000818:Animals; D001105:Archaea / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D008386:Marine Biology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012616:Sea Cucumbers / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D012329:RNA, Bacterial; D012335:RNA, Ribosomal