Pharmacological evidence for neurokinin receptors in murine neuroblastoma C1300 cells.
We found that neurokinin A (NKA) and neurokinin B (NKB) induce an increase in the concentration of intracellular free Ca2+ ([Ca2+]i) in murine neuroblastoma C1300 cells (EC50: NKA 87 +/- 13 nM, NKB 97 +/- 15 nM). Substance P (SP) also caused a transient Ca2+ increase, although the potency of SP was much less than that of NKA and NKB. The increase in [Ca2+]i induced by NKA and NKB was inhibited by SR 48,968, a selective antagonist for NK2, and [beta Ala8]NKA(4-10), a selective agonist for NK2, did not stimulate the increase in [Ca2+]i. NKA- and NKB-induced Ca2+ mobilization was not inhibited by CP-96,345 and [Trp7, beta Ala8]NKA(4-10), selective antagonists for NK1 and NK3, respectively. These results suggested that C1300 cells express endogenous NK2 neurokinin receptors that have different features from known NK2 receptors.
Fukuhara
S
S
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Mukai
H
H
Munekata
E
E
D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D007700:Kinetics; D051379:Mice; D009447:Neuroblastoma; D015288:Neurokinin A / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D015287:Neurokinin B / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010880:Piperidines / Q000494:pharmacology; D018039:Receptors, Tachykinin / Q000032:analysis / Q000037:antagonists & inhibitors / Q000502:physiology*; D013373:Substance P / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D001549:Benzamides; D010880:Piperidines; D018039:Receptors, Tachykinin; D013373:Substance P; C073839:SR 48968; D015288:Neurokinin A; D015287:Neurokinin B; D002118:Calcium