A new method of human cardiac troponin I and troponin T purification.
New rapid and effective procedure for simultaneous purification of human cardiac troponin I and troponin T has been developed. Affinity chromatography on immobilized monoclonal antitroponin I antibody C5 was used for purification of the whole troponin complex with a yield of 120 mg from 100 g of tissue. Isolated troponin I and troponin T (about 20 and 35 mg from 100 g tissue) were obtained by conventionally used ion-exchange chromatography. Antibody C5 recognizes conservative epitope of troponin I, therefore the method is applicable for purification of skeletal and cardiac troponin from a number of different animal species.
Katrukha
A G
AG
Russian Center of Molecular Diagnostics and Medical Care, M. V. Lomonosov Moscow State University.
Bereznikova
A V
AV
Esakova
T V
TV
Filatov
V L
VL
Bulargina
T V
TV
Gusev
N B
NB
D000911:Antibodies, Monoclonal / Q000276:immunology; D015153:Blotting, Western; D002846:Chromatography, Affinity / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D000939:Epitopes / Q000276:immunology; D006801:Humans; D009206:Myocardium / Q000737:chemistry*; D014336:Troponin / Q000276:immunology / Q000302:isolation & purification*; D019210:Troponin I; D020107:Troponin T
D000911:Antibodies, Monoclonal; D000939:Epitopes; D014336:Troponin; D019210:Troponin I; D020107:Troponin T