Somatostatin, galanin and peptide histidine isoleucine in the newborn and adult human trigeminal ganglion and spinal nucleus: immunohistochemistry, neuronal morphometry and colocalization with substance P.
By means of indirect immunofluorescence the neuropeptides somatostatin, galanin and peptide histidine isoleucine were localized in cell bodies, nerve fibres and terminal-like elements in the ganglion and spinal nucleus of the human trigeminal nerve in perinatal and adult ages. No immunoreactivity to vasoactive intestinal polypeptide was observed. In the gasserian ganglion somatostatin-, galanin- and peptide histidine isoleucine-containing neurons and nerve fibres occurred frequently in pre- and full-term newborns, but were scarce to absent in adults. Somatostatin- and galanin-positive pericellular basket-like structures around non-immunoreactive perikarya were observed in newborn specimens. Immunoreactivity to somatostatin, galanin and peptide histidine isoleucine labelled nerve fibers and punctate and felt-like nerve terminals in the pars interpolaris and subnucleus caudalis of the spinal trigeminal nucleus, with immunostaining and distribution patterns characteristic for each peptide. In addition, somatostatin-containing neuronal cell bodies frequently were detected. At variance with those containing somatostatin, the number of galanin- and peptide histidine isoleucine-like immunoreactive elements were dramatically reduced in the adult tissue compared to the newborn one. Double immunostaining revealed that each of the three peptides partially colocalizes with substance P, the degree of coexistence being very low for somatostatin/substance P and high for galanin/substance P and peptide histidine isoleucine/substance P both in the gasserian ganglion and in the spinal nucleus. The results obtained suggest that somatostatin, galanin and peptide histidine isoleucine may play functional roles in primary sensory neurons and at the first synaptic level of the human trigeminal sensory system.
Del Fiacco
M
M
Dipartimento di Citomorfologia, University of Cagliari, Italy.
Quartu
M
M
D000368:Aged; D005260:Female; D019004:Galanin; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000737:chemistry*; D009479:Neuropeptides / Q000032:analysis*; D010451:Peptide PHI / Q000032:analysis; D010455:Peptides / Q000032:analysis; D013004:Somatostatin / Q000032:analysis; D013373:Substance P / Q000032:analysis*; D012668:Trigeminal Ganglion / Q000737:chemistry*; D014279:Trigeminal Nucleus, Spinal / Q000737:chemistry*
D009479:Neuropeptides; D010451:Peptide PHI; D010455:Peptides; D013373:Substance P; D013004:Somatostatin; D019004:Galanin