title: N7-cyanoborane-2'-deoxyguanosine 5'-triphosphate is a good substrate for DNA polymerase.
abstract: The 5'-triphosphate of the boronated nucleoside analog N7-cyanoborane-2'-deoxyguanosine (7bdGTP) was synthesized, and a series of experiments was initiated to assess the potential of the compound to serve as a substrate for DNA polymerases. We show here that 7bdGTP can be incorporated into DNA by Sequenase. The resulting hemiboronated extension products are resistant to cleavage by treatment with either DMS and heat or a number of restriction enzymes. Further, in the polymerase chain reaction, 7bdGTP can be utilized as a substrate for Taq polymerase. Finally, by kinetic analysis, we have found that 7bdGTP is a more efficient substrate for exonuclease-free Klenow than normal dGTP. Thus, the introduction of a cyanoborane moiety to the N7 position of dGTP results in a nucleotide that is accepted in lieu of normal dGTP by a number of DNA polymerases.
authors.affiliation: Department of Chemistry, Duke University, Durham, North Carolina 27708, USA.
authors.forename: K W
authors.initials: KW
authors.lastname: Porter
authors.forename: J
authors.initials: J
authors.lastname: Tomasz
authors.forename: F
authors.initials: F
authors.lastname: Huang
authors.forename: A
authors.initials: A
authors.lastname: Sood
authors.forename: B R
authors.initials: BR
authors.lastname: Shaw
mesh_terms: D001483:Base Sequence; D001896:Boron Compounds / Q000138:chemical synthesis / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004262:DNA Restriction Enzymes; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003848:Deoxyguanine Nucleotides / Q000138:chemical synthesis / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013379:Substrate Specificity; D013463:Sulfuric Acid Esters; D019914:Taq Polymerase
chemical_list: D001896:Boron Compounds; D017931:DNA Primers; D003848:Deoxyguanine Nucleotides; C095850:N7-cyanoborane-2'-deoxyguanosine-5'-triphosphate; D013463:Sulfuric Acid Esters; C029603:deoxyguanosine triphosphate; D019914:Taq Polymerase; C034970:bacteriophage T7 induced DNA polymerase; D004259:DNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes; C007482:dimethyl sulfate