[Inhibitors of NO-synthase and guanylate cyclase block the modulation of cholinoreceptor plasticity in snail neurons by 15-hydroxyeicosatetraenoic acid].
Probable participation of two second messengers, NO and cGMP, in short- and long-latent effects of 15(S)-hydroxy-(5Z,8Z,11Z, 13E)-eicosatetraenoic acid (15-HETE, acyclic eicosanoid) on plasticity of somatic acetylcholine receptors on identified neurons of Helix lucorum was studied using two-electrode voltage clamp technique. It was shown that inhibitor of NO synthase N omega-Methyl-L-Arginine and inhibitors of soluble guanylate cyclase LY-83,583 and methylene blue disturbed short- and long-latent modulatory effects of 15-HETE on depression depth of the inward current evoked by rhythmic acetylcholine application on the neuron soma. We suppose that NO and cGMP participate in short- and long-latent effects of 15-HETE on plasticity of acetylcholine receptors.
Pivovarov
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Egido-Villarreal
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D000109:Acetylcholine / Q000494:pharmacology; D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors*; D000634:Aminoquinolines / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D003864:Depression, Chemical; D017952:Ganglia, Invertebrate / Q000187:drug effects / Q000502:physiology; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors*; D006372:Helix, Snails; D006893:Hydroxyeicosatetraenoic Acids / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D066298:In Vitro Techniques; D008751:Methylene Blue / Q000494:pharmacology; D009473:Neuronal Plasticity / Q000187:drug effects* / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D019001:Nitric Oxide Synthase; D011930:Reaction Time / Q000187:drug effects / Q000502:physiology; D011950:Receptors, Cholinergic / Q000187:drug effects* / Q000502:physiology; D015290:Second Messenger Systems / Q000187:drug effects / Q000502:physiology; D019323:omega-N-Methylarginine
D000634:Aminoquinolines; D006893:Hydroxyeicosatetraenoic Acids; D011950:Receptors, Cholinergic; D019323:omega-N-Methylarginine; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; C041715:6-anilino-5,8-quinolinedione; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D006162:Guanylate Cyclase; D000109:Acetylcholine; D008751:Methylene Blue