A monoclonal antibody against blood forms of Trypanosoma cruzi lyses the parasite in vitro and inhibits host cell invasion.
Monoclonal antibodies (mAbs) of the IgM isotypes were produced from mice immunized with blood forms of Trypansoma cruzi Y strain. Characterization of the epitope recognized by one of the mAbs, 164C11, as well as the effects of this mAb on complement-mediated lysis and host cell invasion are reported. Immunocytochemical analysis showed that the mAb was reactive with various strains of T. cruzi (Y, WSL, and Colombiana) as well as other trypanosomatids. The mAb 164C11 demonstrated a high complement-mediated lytic activity against bloodstream trypomastigotes, being more effective than chronic mouse serum. A protein with an apparent molecular weight of 72 kDa was detected by this mAb on all developmental stages of T. cruzi. Studies using periodate and endoglycosidase treatments suggested that the epitope is not a carbohydrate and seems to be located on the parasite membrane. In addition, preliminary results are presented, suggesting that the 72-kDa protein is involved in adhesion/or internalization of bloodstream trypomastigotes.
Gomes
Y M
YM
Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhaes-FIOCRUZ, Recife-PE, Brazil.
Abath
F G
FG
Furtado
A F
AF
Regis
L N
LN
Nakasawa
M
M
Montenegro
L T
LT
Vouldoukis
I
I
Alfred-Morin
C
C
Monjour
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000494:pharmacology; D002478:Cells, Cultured; D003167:Complement Activation; D000939:Epitopes / Q000276:immunology; D006790:Host-Parasite Interactions; D006825:Hybridomas / Q000276:immunology; D066298:In Vitro Techniques; D051379:Mice; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000502:physiology
D000911:Antibodies, Monoclonal; D000939:Epitopes