Simultaneous immunoaffinity purification of O6-methyl, O6-ethyl-, O6-propyl- and O6-butylguanine and their analysis by gas chromatography/mass spectrometry.
A sensitive and specific method has been developed for the simultaneous analysis of different O6-alkylguanines. The cross-reactivity of two different antibodies raised against O6-methylguanosine and O6-butylguanosine for a series of O6-alkylguanines was exploited for the immunoaffinity purification of biological samples before quantitative analysis by gas chromatography/mass spectrometry. The method can be applied to the detection of O6-alkylguanines in DNA and appears to be useful for studying chemical carcinogen mechanisms in animals and possibly for the detection of human exposure to alkylating agents.
Airoldi
L
L
Laboratory of Molecular Toxicology, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Magagnotti
C
C
Chiappetta
L
L
Bonfanti
M
M
Pastorelli
R
R
Fanelli
R
R
D000478:Alkylation; D000818:Animals; D000906:Antibodies; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006147:Guanine / Q000031:analogs & derivatives* / Q000302:isolation & purification*; D006151:Guanosine / Q000031:analogs & derivatives / Q000276:immunology / Q000302:isolation & purification; D008297:Male; D011817:Rabbits; D012680:Sensitivity and Specificity
D000906:Antibodies; D006151:Guanosine; D006147:Guanine; C046175:6'-O-methylguanosine