Evaluation of bilirubin displacing effect of indomethacin by determination of erythrocyte-bound bilirubin.
The effect of indomethacin on bilirubin binding to human erythrocytes at different bilirubin/albumin molar ratios varying from 0.5-3.0 was studied. Progressive addition of indomethacin produced a linear increase in erythrocyte-bound bilirubin. This increase was more pronounced up to 280 microM drug concentration at all molar ratios being maximum at higher bilirubin/albumin molar ratio. On the other hand, percent increase in erythrocyte-bound bilirubin produced by the drug was found to be maximum at lowest bilirubin/albumin molar ratio and decreased on increasing bilirubin/albumin molar ratio. From these results we conclude that indomethacin induces displacement of bilirubin from albumin, thus increases bilirubin binding to human erythrocytes to a significant degree even at lower concentrations.
Tayyab
S
S
Protein & Enzyme Laboratory, Aligarh Muslim University, Uttar Pradesh, India.
Rao
S Y
SY
D001663:Bilirubin / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology*
D001663:Bilirubin; D007213:Indomethacin