[Characterization of chromosomal rearrangements by in situ hybridization in glioblastoma].
In a case of glioblastoma, the following karyotype was determined: 47, X, - Y, + der(1) t(1;9)(p21;p23), t(1;9)(p21;p23), + 3, + 7, der(9) t(Y;9)(q11;p21), - 13, t(13;16)(p13,p11), del(14)(q11q22). Classical satellite DNAs are mainly located in chromosomes 1, 9, 15, 16 and Y. Because, most of these chromosomes were implicated in the rearrangements, a detailed cytogenetic study was undertaken. This study included in situ hybridization of the satellite and alphoid DNAs of chromosomes 1, 9, 16 and Y combined with various chromosome banding methods (DA-DAPI, quinacrine mustard and R-banding). The data obtained, demonstrated that the breakpoints were always located outside the areas containing the satellite and alphoid DNAs. The situation observed here differs from that reported in breast cancers for which a high proportion of the breakpoints occur within these areas. These findings suggest that in glioblastoma, chromosome rearrangements result from different mechanisms than those implicated in breast cancers. Thus, in cancers, chromosomal instabilities may result from several mechanisms.
Bourgeois
C A
CA
URA 620 CNRS, section de biologie, institut Curie, Paris, France.
Kokalj-Vokac
N
N
Dutrillaux
A M
AM
Poisson
M
M
Delattre
J Y
JY
Vega
F
F
Malfoy
B
B
Dutrillaux
B
B
D001374:Azacitidine / Q000009:adverse effects; D002869:Chromosome Aberrations / Q000235:genetics*; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1; D002885:Chromosomes, Human, Pair 16; D002899:Chromosomes, Human, Pair 9; D004273:DNA, Neoplasm / Q000235:genetics*; D004276:DNA, Satellite; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D006570:Heterochromatin / Q000187:drug effects; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D008745:Methylation; D008875:Middle Aged; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D004276:DNA, Satellite; D006570:Heterochromatin; D001374:Azacitidine