Nitric oxide plays no role in ACTH release induced by interleukin-1 beta, corticotropin-releasing hormone, arginine vasopressin and phorbol myristate acetate in rat pituitary cell cultures.
To investigate the role of nitric oxide (NO) in pituitary ACTH secretion, the effect of a nitric oxide synthase (NOS) inhibitor, N omega-Nitro-L-arginine (Nitro-Arg), on ACTH secretion induced by interleukin (IL)-1 beta, corticotropin releasing hormone (CRH), arginine vasopressin (AVP) and phorbol myristate acetate (PMA) was examined in rat anterior pituitary cell cultures. Nitro-Arg did not affect IL-1 beta-induced ACTH release in pituitary cell cultures incubated with Dulbecco modified Eagle's medium, Basal Medium Eagle or Krebs Ringer bicarbonate-glucose buffer. It did not affect CRH-, AVP- or PMA-induced ACTH release either. These results suggest that NO does not play an important role in ACTH release at the pituitary level.
Hashimoto
K
K
Second Department of Internal Medicine, Kochi Medical School, Japan.
Nishioka
T
T
Tojo
C
C
Takao
T
T
D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D001127:Arginine Vasopressin / Q000494:pharmacology; D002478:Cells, Cultured; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology; D007375:Interleukin-1 / Q000494:pharmacology; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D019001:Nitric Oxide Synthase; D019335:Nitroarginine; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D007375:Interleukin-1; D001127:Arginine Vasopressin; D019335:Nitroarginine; D009569:Nitric Oxide; D000324:Adrenocorticotropic Hormone; D003346:Corticotropin-Releasing Hormone; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D013755:Tetradecanoylphorbol Acetate