The integrin receptor alpha 8 beta 1 mediates interactions of embryonic chick motor and sensory neurons with tenascin-C.
This paper identifies a neuronal receptor for tenascin-C (tenascin/cytotactin), an extracellular matrix protein that has previously been detected in developing sensory and motor neuron pathways and has been shown to regulate cell migration in the developing CNS. Antibodies specific for each subunit of the integrin alpha 8 beta 1 are used to demonstrate that alpha 8 beta 1 mediates neurite outgrowth of embryonic sensory and motor neurons on this extracellular matrix protein. In addition, expression of alpha 8 in K562 cells results in surface expression of alpha 8 beta 1 heterodimers that are shown to promote attachment of this cell line to tenascin. The major domain in tenascin that mediates neurite outgrowth is shown to be localized to fibronectin type III repeats 6-8.
Varnum-Finney
B
B
Department of Physiology, University of California, San Francisco 94143-0724, USA.
Venstrom
K
K
Muller
U
U
Kypta
R
R
Backus
C
C
Chiquet
M
M
Reichardt
L F
LF
D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D002642:Chick Embryo; D005109:Extracellular Matrix / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000378:metabolism* / Q000494:pharmacology; D005353:Fibronectins / Q000737:chemistry; D005727:Ganglia, Spinal / Q000648:ultrastructure; D007163:Immunosorbent Techniques; D039001:Integrin alpha Chains; D016023:Integrins / Q000032:analysis / Q000737:chemistry; D046911:Macromolecular Substances; D009046:Motor Neurons / Q000378:metabolism* / Q000648:ultrastructure; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D009475:Neurons, Afferent / Q000378:metabolism* / Q000648:ultrastructure; D011946:Receptors, Antigen / Q000032:analysis / Q000502:physiology*; D012091:Repetitive Sequences, Nucleic Acid; D019063:Tenascin
D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D039001:Integrin alpha Chains; D016023:Integrins; D046911:Macromolecular Substances; D011946:Receptors, Antigen; D019063:Tenascin; C028641:Tn receptor; C093856:integrin alpha8