Dibutyryl cyclic adenosine monophosphate stimulates the sodium pump in rabbit renal cortical tubules.
The elevation in oxygen consumption (QO2) observed following addition of the sodium ionophore nystatin in suspensions of rabbit renal proximal tubules was significantly increased by 1 mM dibutyryl cyclic adenosine monophosphate (db-cAMP). The QO2 after subsequent addition of strophanthidin to block the sodium pump was unaffected by db-cAMP. However, 10 microM forskolin in the presence of 100 microM IBMX had no significant effect on the QO2 observed following addition of either nystatin or strophanthidin. Nevertheless, we can conclude that db-cAMP does stimulate the sodium pump activity independently of sodium transport mechanisms in the rabbit renal proximal tubule.
Beck
J S
JS
Groupe de Recherche en Transport Membranaire, Université de Montréal, Qué., Canada.
Marsolais
M
M
Nöel
J
J
Breton
S
S
Laprade
R
R
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000378:metabolism / Q000494:pharmacology*; D002469:Cell Separation; D003078:Colchicine / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000378:metabolism; D009761:Nystatin / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism*; D013327:Strophanthidin / Q000494:pharmacology
D009761:Nystatin; D005576:Colforsin; D003994:Bucladesine; D013327:Strophanthidin; D000242:Cyclic AMP; D000254:Sodium-Potassium-Exchanging ATPase; D003078:Colchicine; D015056:1-Methyl-3-isobutylxanthine