[Antigenic determination of human anti-rabies vaccine against viral street strains common in the wild animal population in Poland].
The aim of the study was to compare the antigen properties of a vaccine strain with street strains isolated from various animal hosts throughout the country. Investigation was carried out using monoclonal antibodies against NC protein. Also, two tests were carried out: the modified NIH test for potency and the neutralization test using the sera of people vaccinated against rabies (PM vaccine strain). The investigated street strains were used in both tests as the challenge viruses. A suspension of these strains diluted five times made it possible to avoid extreme values of animal survival (0% or 100%) what, consequently, made calculation of the LD50 value easier. A different rabies virus serotype (EBLI virus) in the population of insectivore bats Eptesicus serotinus and antigen variants within the first serotype, having common epitopes with strains of the vaccine virus SAD B19 and the polar rabies virus, were found to be present throughout the country. The concentrated and purified vaccine containing the PM virus did not protect mice against infection with strains of viruses isolated from bats (protection index 10 and lower). For the remaining strains, depending on the animal source of their isolation, the protection index ranged from 10 to 1000 and higher. The properties neutralizing a dose of 5 i.u./ml of serum from the subject inoculated with the vaccine containing the PM strain were similar for all the investigated strains; 0,5 i.u./ml did not neutralize the strain isolated from a racoon dog.
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Zakład Epidemiologii PZH w Warszawie.
D000818:Animals; D000835:Animals, Wild; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D002685:Chiroptera; D004199:Disease Vectors; D004285:Dogs; D000939:Epitopes; D006801:Humans; D051379:Mice; D009500:Neutralization Tests; D011818:Rabies / Q000276:immunology / Q000517:prevention & control / Q000635:transmission / Q000821:virology*; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D012703:Serotyping; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D011819:Rabies Vaccines