Characterization of C6-10A glioma cells highly responsive to beta-adrenergic receptor agonist-induced NGF synthesis/secretion.
A subline of rat C6 glioma cells, C6-10A cells, in which epinephrine can induce nerve growth factor (NGF) synthesis/secretion, was isolated. C6-10A cells have retained their sensitivity to epinephrine for more than 2 years in a medium containing 0.5% fetal calf serum (FCS) but easily lose it in 10% FCS. C6-10A cells are S-100- and glial fibrillary acidic protein-positive, and the doubling time is about 60 h in the medium containing 0.5% FCS and about 20 h in 10% FCS. Epinephrine induced NGF synthesis/secretion prominently in serum-free cultures of C6-10A cells and in cultures with a high cell density, but not in serum-containing cultures. The induction did not occur with parent C6 cells under the appropriate conditions in C6-10A cells. NGF secretion was induced by catecholaminergic compounds in the following order isoproterenol > epinephrine = norepinephrine >> dopamine. The induction caused by epinephrine was blocked by propranolol (beta-blocker) but not by phentolamine (alpha-blocker). Various compounds that activate the adenylate cyclase system also induced NGF synthesis/secretion. These results indicate that C6-10A cells are astrocytes that are highly responsive to beta-adrenergic receptor agonists, which stimulate NGF synthesis/secretion via receptors coupled with adenylate cyclase machinery. These cells may be a useful aid in studying the mechanism of NGF synthesis/secretion.
Fukumoto
H
H
Department I, Takeda Chemical Ind., Ltd., Osaka, Japan.
Kakihana
M
M
Suno
M
M
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001769:Blood; D003994:Bucladesine / Q000494:pharmacology; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002772:Cholera Toxin / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology*; D005353:Fibronectins / Q000032:analysis; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D005910:Glioma; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D007700:Kinetics; D004676:Myelin Basic Protein / Q000032:analysis; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000494:pharmacology; D051381:Rats; D009418:S100 Proteins / Q000032:analysis; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000317:Adrenergic alpha-Antagonists; D000319:Adrenergic beta-Antagonists; D003470:Culture Media; D005353:Fibronectins; D005904:Glial Fibrillary Acidic Protein; D004676:Myelin Basic Protein; D009414:Nerve Growth Factors; D018377:Neurotransmitter Agents; D009418:S100 Proteins; D005576:Colforsin; D003994:Bucladesine; D002772:Cholera Toxin; D015056:1-Methyl-3-isobutylxanthine; D004837:Epinephrine