Usefulness of the AMeX method for immunostaining with antikeratin antibodies.
We performed an immunohistochemical study with 11 antikeratin antibodies using the newly developed AMeX (acetone-methylbenzoate-xylene) tissue processing method. Specimens processed with this method showed almost as good preservation and morphological detail as routinely processed, formalin-fixed and paraffin-embedded tissue specimens, and as good preservation of antigenicity as fresh frozen tissue specimens. Thus, we propose the wide use of this method in dermatology.
Ansai
S I
SI
Department of Dermatology, Yamagata University School of Medicine, Japan.
Koseki
S
S
Hozumi
Y
Y
Kondo
S
S
D000096:Acetone; D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D001565:Benzoates; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000276:immunology*; D008297:Male; D012867:Skin; D014021:Tissue Preservation / Q000379:methods*; D014992:Xylenes
D000911:Antibodies, Monoclonal; D001565:Benzoates; D014992:Xylenes; D000096:Acetone; C044605:methyl benzoate; D007633:Keratins