Monoclonal antibodies to human heart fatty acid-binding protein.
Heart-type fatty acid-binding protein (H-FABP), a 15 kDa non-enzymatic protein, which is abundantly present in heart and some skeletal muscles, was recently found to be a useful plasma marker for acute myocardial infarction. The BIAcore biosensor technology was used to raise and characterize a panel of 13 monoclonal antibodies against human H-FABP which did not crossreact with other FABP types of human origin. The kinetics of association and dissociation between FABP and the monoclonal antibodies was studied. By pairwise mapping several distinct epitopes could be identified.
Roos
W
W
Roche Diagnostic Systems, division of F. Hoffmann-La Roche Ltd., Basel, Switzerland.
Eymann
E
E
Symannek
M
M
Duppenthaler
J
J
Wodzig
K W
KW
Pelsers
M
M
Glatz
J F
JF
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D015374:Biosensing Techniques; D002352:Carrier Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000276:immunology / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009206:Myocardium / Q000737:chemistry; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D025521:Tumor Suppressor Proteins
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D000939:Epitopes; C107608:FABP7 protein, human; C082667:Fabp5 protein, mouse; C086936:Fabp7 protein, mouse; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D025521:Tumor Suppressor Proteins