Identification of CD34+ cord blood cells and their subpopulations in preterm and term neonates using three-color flow cytometry.
Three-color flow cytometry was used to identify CD34+ cord blood (CB) hematopoietic progenitors of preterm (n = 13) and term (n = 18) neonates. The frequency of CD34+ CB cells gated on the lymphocyte fraction in term neonates is about half the frequency of preterm neonates (mean 1.84 +/- 0.8 vs. 3.49 +/- 1.8, p < 0.005). The difference of absolute CD34+ cells between the 2 groups did not reach statistical significance (premature 169 +/- 161 vs. mature 108 +/- 62.6 x 10(6) cells/l). The proportion of myeloid lineage-committed CD34+ cells coexpressing the CD33 antigen in preterm neonates does not significantly differ from that found in term neonates. However, in preterm neonates a higher fraction of erythroid lineage-committed CD34+ cells coexpresses the CD71 antigen as compared with term neonates (mean 28.7 +/- 14.1 vs. 11.4 +/- 5.7, p < 0.001). The positive correlation between gestational age and the ratio of myeloid/erythroid lineage-specific progenitor cells (r = 0.61, n = 31, p < 0.0005) suggests gestational changes in lineage commitment of CD34+ cells.
Meister
B
B
Department of Pediatrics, University of Innsbruck, Austria.
Tötsch
M
M
Mayr
A
A
Widschwendter
M
M
Huter
O
O
Sperl
W
W
D015703:Antigens, CD / Q000097:blood*; D018952:Antigens, CD34; D000944:Antigens, Differentiation, B-Lymphocyte / Q000097:blood; D015214:Antigens, Differentiation, Myelomonocytic / Q000097:blood; D004906:Erythrocyte Count; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000276:immunology; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods*; D006098:Granulocytes / Q000166:cytology / Q000276:immunology; D006684:HLA-DR Antigens / Q000097:blood; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000097:blood*; D007958:Leukocyte Count; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D011990:Receptors, Transferrin; D063268:Sialic Acid Binding Ig-like Lectin 3
D015703:Antigens, CD; D018952:Antigens, CD34; D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic; C571163:CD33 protein, human; C068126:CD71 antigen; D006684:HLA-DR Antigens; D011990:Receptors, Transferrin; D063268:Sialic Acid Binding Ig-like Lectin 3