title: Induction of macrophage procoagulant activity by murine hepatitis virus strain 3: role of tyrosine phosphorylation.
abstract: The induction of a unique macrophage procoagulant molecule by murine hepatitis virus strain 3 correlates with the severity of viral hepatitis. The role of tyrosine phosphorylation in the signalling pathway leading to procoagulant expression was studied. Murine hepatitis virus strain 3 initiated a rapid increase in phosphotyrosine accumulation. Tyrosine kinase inhibition precluded this increase and abrogated expression of the virus-induced procoagulant mouse fibrinogen-like protein (musfiblp) gene. These findings suggest that manipulation of this signalling pathway in vivo might represent a novel approach to treating this disease.
authors.affiliation: Department of Surgery, University of Toronto, Canada.
authors.forename: A P
authors.initials: AP
authors.lastname: Dackiw
authors.forename: K
authors.initials: K
authors.lastname: Zakrzewski
authors.forename: A B
authors.initials: AB
authors.lastname: Nathens
authors.forename: P Y
authors.initials: PY
authors.lastname: Cheung
authors.forename: R
authors.initials: R
authors.lastname: Fingerote
authors.forename: G A
authors.initials: GA
authors.lastname: Levy
authors.forename: O D
authors.initials: OD
authors.lastname: Rotstein
mesh_terms: D000818:Animals; D016227:Benzoquinones; D002396:Catechols / Q000494:pharmacology; D002478:Cells, Cultured; D005260:Female; D019833:Genistein; D006524:Hepatitis, Viral, Animal / Q000276:immunology / Q000503:physiopathology; D007529:Isoflavones / Q000494:pharmacology; D007700:Kinetics; D047029:Lactams, Macrocyclic; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000502:physiology* / Q000821:virology; D051379:Mice; D006517:Murine hepatitis virus / Q000145:classification / Q000502:physiology*; D009570:Nitriles / Q000494:pharmacology; D010766:Phosphorylation; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011809:Quinones / Q000494:pharmacology; D017828:Rifabutin / Q000031:analogs & derivatives; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism*; D020032:Tyrphostins
chemical_list: D016227:Benzoquinones; D002396:Catechols; D007529:Isoflavones; D047029:Lactams, Macrocyclic; D009570:Nitriles; D011809:Quinones; D020032:Tyrphostins; C060152:tyrphostin 47; D017828:Rifabutin; D019000:Phosphotyrosine; D014443:Tyrosine; C020754:herbimycin; D019833:Genistein; D011505:Protein-Tyrosine Kinases