Further identification of human plasma glycoproteins interacting with the galactose-specific lectin Jacalin.
In this report we show that Jacalin binds the heme-binding protein hemopexin and the C4b-binding protein sgp120 in human plasma. The interaction of Jacalin with hemopexin confirms that a single O-linked oligosaccharide is sufficient to mediate binding of a protein to this lectin. Retention of sgp120 by immobilized Jacalin demonstrated that this protein was O-glycosylated and, therefore, clearly different from another C4b-binding protein, the complement protein C2 which is physicochemically similar but exclusively N-glycosylated. In addition, Jacalin was also shown to bind several proteolytic enzymes which remain to be identified.
Pilatte
Y
Y
INSERM unité 139, Institut Mondor de Médecine Moléculaire (IM3) CHU H. Mondor, Créteil, France.
Rostoker
G
G
Violleau
K
K
Basta
M
M
Hammer
C H
CH
D000595:Amino Acid Sequence; D002240:Carbohydrate Sequence; D002352:Carrier Proteins / Q000378:metabolism*; D003169:Complement Inactivator Proteins; D005690:Galactose / Q000378:metabolism*; D006023:Glycoproteins; D006466:Hemopexin / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D037102:Lectins / Q000378:metabolism*; D008969:Molecular Sequence Data; D037121:Plant Lectins; D011485:Protein Binding
D002352:Carrier Proteins; D003169:Complement Inactivator Proteins; D006023:Glycoproteins; D037102:Lectins; D037121:Plant Lectins; C043696:jacalin; D006466:Hemopexin; D005690:Galactose