title: Novel isoform of beta 1 integrin expressed in skeletal and cardiac muscle.
abstract: We describe a novel isoform of the beta 1 integrin subunit called beta 1D, which contains a unique cytoplasmic domain, and is expressed specifically in skeletal and cardiac muscle. The beta 1D isoform arises from splicing into the final transcript of an additional exon located between exons 6 and 7. The nucleotide sequence of beta 1D predicts a cytoplasmic domain of 50 amino acids in which the last 21 amino acids of the beta 1A integrin isoform are replaced by a related sequence of 24 amino acids. A beta 1D-specific anti-peptide polyclonal antibody was prepared and immunoprecipitation of tissue extracts with subsequent immunoblotting showed expression of beta 1D isoform only in striated muscle cells.
authors.affiliation: Department of Cell Biology, University of Alabama at Birmingham 35294, USA.
authors.forename: N I
authors.initials: NI
authors.lastname: Zhidkova
authors.forename: A M
authors.initials: AM
authors.lastname: Belkin
authors.forename: R
authors.initials: R
authors.lastname: Mayne
mesh_terms: D000328:Adult; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D018244:Chromosomes, Artificial, Yeast; D004247:DNA; D005091:Exons; D005260:Female; D006801:Humans; D019012:Integrin beta1; D016023:Integrins / Q000235:genetics*; D007438:Introns; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
chemical_list: D019012:Integrin beta1; D016023:Integrins; D004247:DNA