Influence of centrifugation on the infectivity of Chlamydia pneumoniae IOL-207.
The influence of centrifugation on the infectivity of Chlamydia pneumoniae IOL-207 was compared with seven serovars of C. trachomatis biovar trachoma, the three serovars of C. trachomatis biovar lymphogranuloma and four C. psittaci strains. Prolonged centrifugation from 1 to 4 hours resulted in a 3-fold increase in the infectivity of C. pneumoniae and variable or no increase in the infectivity of the other Chlamydia strains studied. These findings indicate that a 4 hour centrifugation of C. pneumoniae is recommended to improve the isolation procedures of this organism in cell cultures.
Donati
M
M
Institute of Microbiology, University of Bologna, Italy.
Graciotti
C
C
Di Francesco
A
A
Pavan
G
G
Bui
C
C
Cevenini
R
R
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001431:Bacteriological Techniques; D002460:Cell Line; D002498:Centrifugation; D002692:Chlamydia trachomatis / Q000254:growth & development / Q000472:pathogenicity; D016993:Chlamydophila pneumoniae / Q000254:growth & development / Q000472:pathogenicity*; D002691:Chlamydophila psittaci / Q000254:growth & development / Q000472:pathogenicity; D005455:Fluorescent Antibody Technique; D002479:Inclusion Bodies / Q000276:immunology / Q000378:metabolism; D013997:Time Factors
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins