Lipopolysaccharide treatment in vivo induces tissue expression of GTP cyclohydrolase I mRNA.
A significant induction of GTP cyclohydrolase I (GTPCH) mRNA was observed in lung, heart and kidney of rats treated with lipopolysaccharide (LPS; 10 mg/kg i.v.). GTPCH mRNA levels in liver were high even in untreated rats, and remained elevated after LPS treatment. Parallel induction of nitric oxide synthase (NOS) mRNA was observed in these tissues of LPS-treated rats. Our results demonstrate induction of GTPCH mRNA after LPS treatment in vivo and provide molecular evidence for the increased GTPCH activity which may up-regulate NOS activity in vivo.
Hattori
Y
Y
Department of Endocrinology, Dokkyo University School of Medicine, Tochigi, Japan.
Oka
M
M
Kasai
K
K
Nakanishi
N
N
Shimoda
S I
SI
D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis; D000818:Animals; D001483:Base Sequence; D004790:Enzyme Induction / Q000187:drug effects; D006136:GTP Cyclohydrolase / Q000096:biosynthesis* / Q000235:genetics; D007668:Kidney / Q000201:enzymology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D009206:Myocardium / Q000201:enzymology; D019001:Nitric Oxide Synthase; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar
D008070:Lipopolysaccharides; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D006136:GTP Cyclohydrolase