Localization of a sequential B-epitope in the VP2 protein of hepatitis A virus.
A set of synthetic peptides derived from the capsid protein of hepatitis A virus was used to search for B-epitopes. Peptides from the 115-139 region of the VP1 protein, from the 69-99 region of the VP2 protein and peptide 137-150 from the VP3 protein were found to react with monoclonal and polyclonal anti-HAV antibodies. MAPs based on 64-80 and 66-80 fragments of VP3 were reactive as well. Peptides, their conjugates with protein carriers and MAPs were used for antipeptide antibody production. Only free peptide 69-99 from the VP2 protein caused formation of HAV binding antibodies.
Kulik
L N
LN
Institute of Bioorganic Chemistry, Byelorussian Academy of Sciences, Minsk.
Ivanov
V S
VS
Tchikin
L D
LD
Ostrovsky
A G
AG
Ivanov
V T
VT
D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D002213:Capsid / Q000737:chemistry*; D000939:Epitopes / Q000737:chemistry; D006507:Hepatovirus / Q000737:chemistry* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis
D000939:Epitopes; D010455:Peptides