The recognition of three different epitopes for the H-type 2 human blood group determinant by lectins of Ulex europaeus, Galactia tenuiflora and Psophocarpus tetragonolobus (winged bean).
The chemical mapping of the regions of H-type 2 human blood group-related trisaccharide (Fuc alpha (1-2)Gal beta (1-4)GlcNAc beta Me) that are recognized by three different lectins, the so-called epitopes, are reviewed together with an account of how and why oligosaccharides form specific complexes with proteins as presently viewed in this laboratory. The occasion is used to report the synthesis of the various mono-O-methyl derivatives of the above trisaccharide that were used in these investigations. Also, Fuc alpha (1-2)Gal beta (1-4)Xyl beta Me was synthesized in order to examine whether or not the hydroxymethyl group of the GlcNAc residue participates in the binding reaction.
Du
M H
MH
Department of Chemistry, University of Alberta, Edmonton, Canada.
Spohr
U
U
Lemieux
R U
RU
D000017:ABO Blood-Group System / Q000276:immunology / Q000378:metabolism*; D001665:Binding Sites; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000378:metabolism*; D007887:Fabaceae; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010946:Plants, Medicinal
D000017:ABO Blood-Group System; D000939:Epitopes; D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins; C049304:winged bean lectin