Genotoxicity of m-phenylenediamine and 2-aminofluorene in Salmonella typhimurium and human lymphocytes with and without plant activation.
The promutagenic arylamines, m-phenylenediamine (mPDA) and 2-aminofluorene (2-AF), were evaluated for their genotoxicity in Salmonella typhimurium strain YG1024 and in human lymphocytes. These agents were assayed with and without TX1MX plant activation mix. Both arylamines without activation were refractory in S. typhimurium, demonstrating that plant activation was required for the generation of their ultimate mutagenic metabolites. However, using the alkaline single-cell gel/Comet assay, both mPDA and 2-AF directly induced DNA damage in human lymphocytes. This effect was reduced when the human cells were treated with the arylamine plus TX1MX. mPDA with or without plant activation was not toxic to the exposed cells. However, at concentrations over 80 microM, 2-AF was toxic to lymphocytes. This toxic response was eliminated by incubation with TX1MX. mPDA and 2-AF were plant-activated into mutagens for S. typhimurium. However, these plant-activated products had a reduced genotoxic potency in human lymphocytes.
Plewa
M J
MJ
Institute for Environmental Studies, University of Illinois at Urbana-Champaign 61801-4723, USA.
Wagner
E D
ED
Yu
T W
TW
Anderson
D
D
D000328:Adult; D002470:Cell Survival / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects / Q000235:genetics; D005260:Female; D005449:Fluorenes / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation / Q000187:drug effects / Q000235:genetics; D010655:Phenylenediamines / Q000633:toxicity*; D010936:Plant Extracts / Q000494:pharmacology; D010947:Plants, Toxic; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism
D005449:Fluorenes; D009153:Mutagens; D010655:Phenylenediamines; D010936:Plant Extracts; C012177:2-aminofluorene; C008381:3-phenylenediamine