Parainfluenza virus type-3 infection attenuates the respiratory effects of antigen challenge in sensitized guinea pigs.
Respiratory viral infections not only exacerbate asthma symptoms but may also be important in the pathogenesis of the disease. We therefore explored the effects of respiratory viral infection on the respiratory response of sensitized guinea pigs to antigen challenge. Lung tissue obtained from uninfected guinea pigs sensitized to ovalbumin aerosol released histamine upon incubation with the antigen in vitro. After antigen challenge in vivo, sensitized animals had significantly greater numbers of eosinophils in their bronchoalveolar lavage fluid than did nonsensitized animals and exhibited airway hyperresponsiveness to methacholine aerosol. When ovalbumin sensitization was initiated 7 days after inoculation with parainfluenza virus type-3 (PI-3), antigen challenge elicited little histamine release from infected lung tissue in vitro. Likewise, subsequent to antigen challenge in vivo, animals failed to exhibit airway hyperresponsiveness or an increased eosinophil population in bronchoalveolar lavage fluid. Similar effects were observed when sensitization was begun 19 days after PI-3 virus inoculation. The mechanism(s) responsible for the attenuated responses to antigen in PI-3 infected animals are unknown but may involve virus-induced effects on immune cells.
Kudlacz
E M
EM
Marion Merrell Dow Research Institute, Cincinnati, Ohio 45215, USA.
Knippenberg
R W
RW
D000336:Aerosols; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count / Q000187:drug effects; D004195:Disease Models, Animal; D004417:Dyspnea / Q000175:diagnosis / Q000276:immunology; D004804:Eosinophils / Q000166:cytology / Q000187:drug effects; D006168:Guinea Pigs; D006636:Histamine Release / Q000187:drug effects / Q000276:immunology; D066298:In Vitro Techniques; D008297:Male; D016210:Methacholine Chloride / Q000008:administration & dosage / Q000633:toxicity; D010047:Ovalbumin / Q000276:immunology / Q000633:toxicity; D010224:Parainfluenza Virus 3, Human / Q000187:drug effects / Q000276:immunology*; D018184:Paramyxoviridae Infections / Q000276:immunology*
D000336:Aerosols; D016210:Methacholine Chloride; D010047:Ovalbumin