Elevated expression of H type GDP-L-fucose:beta-D-galactoside alpha-2-L-fucosyltransferase is associated with human colon adenocarcinoma progression.
GDP-L-fucose:beta-D-galactoside alpha-2-L-fucosyltransferase (EC 2.4.1.69) is a key enzyme in the biosynthesis of fucosylated type 1 and 2 lactoseries structures, such as Lewis b and the H type 2 and Lewis Y, respectively, that are accumulated in colon adenocarcinoma. Analysis of the mRNA transcript level for the human H gene-encoded beta-D-galactoside alpha-2-L-fucosyltransferase revealed 40- and 340-fold increases in the mRNA levels in all adenocarcinomas and tumor cell lines, respectively, compared to normal colon mucosa where a low level of mRNA transcript was detected. A variable increase in mRNA transcript levels was observed in 50% of adenomatous polyps. Nucleotide sequence analysis of the protein coding region of the cDNAs derived from normal colon, adenoma, and colon adenocarcinoma revealed 100% homology, suggesting that there are no tumor-associated allelic variations within the H beta-D-galactoside alpha-2-L-fucosyltransferase cDNA. These results suggest that beta-D-galactoside alpha-2-L-fucosyltransferase expression highly correlates with malignant progression of colon adenocarcinoma.
Sun
J
J
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104-4268, USA.
Thurin
J
J
Cooper
H S
HS
Wang
P
P
Mackiewicz
M
M
Steplewski
Z
Z
Blaszczyk-Thurin
M
M
D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D000818:Animals; D001483:Base Sequence; D002241:Carbohydrates / Q000276:immunology; D003001:Cloning, Molecular; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000473:pathology; D017931:DNA Primers; D018076:DNA, Complementary; D018450:Disease Progression; D000939:Epitopes / Q000032:analysis; D005647:Fucosyltransferases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D014162:Transfection; D014407:Tumor Cells, Cultured; D000097631:Galactoside 2-alpha-L-fucosyltransferase
D002241:Carbohydrates; D017931:DNA Primers; D018076:DNA, Complementary; D000939:Epitopes; D005647:Fucosyltransferases; D012333:RNA, Messenger; D000097631:Galactoside 2-alpha-L-fucosyltransferase