title: Field evaluation of transgenic potato plants expressing an antisense granule-bound starch synthase gene: increase of the antisense effect during tuber growth.
abstract: Transgenic plants of a tetraploid potato cultivar were obtained in which the amylose content of tuber starch was reduced via antisense RNA-mediated inhibition of the expression of the gene encoding granule-bound starch synthase (GBSS). GBSS is one of the key enzymes in the biosynthesis of starch and catalyses the formation of amylose. The antisense GBSS genes, based on the full-length GBSS cDNA driven by the 35S CaMV promoter or the potato GBSS promoter, were introduced into the potato genome by Agrobacterium tumefaciens-mediated transformation. Expression of each of these genes resulted in the complete inhibition of GBSS gene expression, and thus in the production of amylose-free tuber starch, in mature field-grown plants originating from rooted in vitro plantlets of 4 out of 66 transgenic clones. Clones in which the GBSS gene expression was incompletely inhibited showed an increase of the extent of inhibition during tuber growth. This is likely to be due to the increase of starch granule size during tuber growth and the specific distribution pattern of starch components in granules of clones with reduced GBSS activity. Expression of the antisense GBSS gene from the GBSS promoter resulted in a higher stability of inhibition in tubers of field-grown plants as compared to expression from the 35S CaMV promoter. Field analysis of the transgenic clones indicated that inhibition of GBSS gene expression could be achieved without significantly affecting the starch and sugar content of transgenic tubers, the expression level of other genes involved in starch and tuber metabolism and agronomic characteristics such as yield and dry matter content.
authors.affiliation: Department of Plant Breeding, Wageningen Agricultural University, The Netherlands.
authors.forename: A G
authors.initials: AG
authors.lastname: Kuipers
authors.forename: W J
authors.initials: WJ
authors.lastname: Soppe
authors.forename: E
authors.initials: E
authors.lastname: Jacobsen
authors.forename: R G
authors.initials: RG
authors.lastname: Visser
mesh_terms: D000688:Amylose / Q000032:analysis / Q000096:biosynthesis*; D002241:Carbohydrates / Q000032:analysis; D004269:DNA, Bacterial; D005069:Evaluation Studies as Topic; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D007455:Iodine; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development; D030821:Plants, Genetically Modified; D016372:RNA, Antisense / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D012107:Research Design; D011198:Solanum tuberosum / Q000737:chemistry / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D013194:Staining and Labeling; D013213:Starch / Q000737:chemistry / Q000302:isolation & purification; D013214:Starch Synthase / Q000096:biosynthesis / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D002241:Carbohydrates; D004269:DNA, Bacterial; D016372:RNA, Antisense; D012333:RNA, Messenger; C036483:T-DNA; D013213:Starch; D000688:Amylose; D007455:Iodine; D013214:Starch Synthase