Lipopolysaccharide recovery restores susceptibility levels towards beta-lactams in Serratia marcescens.
O-side chain-defective spontaneous mutants of Serratia marcescens, selected by phage resistance, showed lower MICs against various beta-lactams than did their parental strains. The recovery of their ability to produce O-antigen restored the original MIC values, as well as phage susceptibility. The permeability coefficients of wild-type, O- mutants, and revertants, demonstrated that O-antigen modifies the permeability of antibiotics in S. marcescens.
Palomar
J
J
Department of Microbiology and Parasitology, University of Barcelona, Spain.
Puig
M
M
Montilla
R
R
Lorén
J G
JG
Viñas
M
M
D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000502:physiology; D008826:Microbial Sensitivity Tests; D009154:Mutation; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000502:physiology*; D012706:Serratia marcescens / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D047090:beta-Lactams