Boosted systemic immune and local responsiveness after intestinal inflammation in orally sensitized guinea pigs.
OBJECTIVE
Intestinal inflammation resulting in disruption of the mucosal barrier function has been proposed as a cause of increased incidence of allergic diseases. This study was designed to evaluate whether intestinal inflammation is able to change the immune responsiveness to sensitization and antigen challenge responses.


METHODS
Guinea pigs orally sensitized to cow's milk proteins were either treated or not treated with trinitrobenzenesulfonic acid (TNBS) to induce intestinal inflammation and compared with control animals (not sensitized). Systemic immune and local responsiveness to antigen challenge were assessed by measuring antibody serum titers, colonic fluid secretion, mucosal histamine level, and mucus depletion. Intestinal permeability was evaluated from 51Cr-ethylenediaminetetraacetic acid (EDTA) recovery and beta-lactoglobulin serum level.


RESULTS
Immunoglobulin E titers were higher in TNBS-treated animals than in non-TNBS-treated sensitized animals. Antigen challenge in TNBS-treated animals induced a fourfold increase of colonic secretion and greater histamine and mucus depletion than in non-TNBS-treated animals. Permeability to 51Cr-EDTA increased 5 days after TNBS treatment but was unchanged after antigen challenge. In contrast to controls, beta-lactoglobulin was not detected in the sera of challenged sensitized and TNBS-treated animals.


CONCLUSIONS
Intestinal inflammation increasing gut permeability enhances the sensitization process. Therefore, local anaphylactic reactions are exacerbated after antigen challenge.
Fargeas
M J
MJ
Department of Pharmacology, Institut National de la Recherche Agronomique, Toulouse, France.
Theodorou
V
V
More
J
J
Wal
J M
JM
Fioramonti
J
J
Bueno
L
L
D000704:Analysis of Variance; D000818:Animals; D002463:Cell Membrane Permeability; D003106:Colon / Q000378:metabolism; D004492:Edetic Acid / Q000493:pharmacokinetics; D006168:Guinea Pigs; D006636:Histamine Release; D007114:Immunization; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D015212:Inflammatory Bowel Diseases / Q000139:chemically induced / Q000276:immunology* / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007782:Lactoglobulins / Q000493:pharmacokinetics; D008297:Male; D016269:Milk Hypersensitivity / Q000276:immunology; D008894:Milk Proteins / Q000276:immunology; D009093:Mucus / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid
D007074:Immunoglobulin G; D007782:Lactoglobulins; D008894:Milk Proteins; D007073:Immunoglobulin E; D014302:Trinitrobenzenesulfonic Acid; D004492:Edetic Acid