Immunocytochemical study of NA+ K(+)-ATPase alpha 1 and beta 1 subunits in human and rat normal hepatocytes using confocal microscopy.
Hepatic Na+ K(+)-ATPase was recently shown to be composed of two alpha 1 and beta 1 subunits similar to that of kidney. Its localization on hepatocyte plasma membranes was not clearly established. We have studied the localization of alpha 1 and beta 1 subunits using immunocytochemical method and confocal microscopy. In accordance with previous cytochemical findings, the catalytic alpha 1 subunit was distributed in basolateral and bile canalicular membranes of hepatocytes. The beta 1 subunit was not demonstrated, due to its very low amount in the liver. The controversy about the bile canalicular localization was discussed.
Benkoël
L
L
Laboratoire de Pathologie Cellulaire et Métabolique du Foie, Faculté de Médecine, Marseille, France.
Benoliel
A M
AM
Brisse
J
J
Sastre
B
B
Bongrand
P
P
Chamlian
A
A
D000818:Animals; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000201:enzymology*; D008297:Male; D018613:Microscopy, Confocal; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis* / Q000737:chemistry
D000254:Sodium-Potassium-Exchanging ATPase