title: Stability of ascorbic acid, carotenoids, retinol, and tocopherols in plasma stored at -70 degrees C for 4 years.
abstract: Aliquots from 40 plasma pools preserved with metaphosphoric acid were assayed for their ascorbic acid values after 12, 24, and 42 months of storage at -70 degrees C. Similarly, aliquots from 16 plasma pools were assayed for values of retinol, several carotenoids, and two tocopherols at 15.5, 27.5, and 51.5 months of storage at -70 degrees C. There were no indications of important losses of these antioxidant micronutrients during storage from the first to the last assay.
authors.affiliation: Training Center for Public Health Research, Hagerstown, Maryland 21742-2067, USA.
authors.forename: G W
authors.initials: GW
authors.lastname: Comstock
authors.forename: E P
authors.initials: EP
authors.lastname: Norkus
authors.forename: S C
authors.initials: SC
authors.lastname: Hoffman
authors.forename: M W
authors.initials: MW
authors.lastname: Xu
authors.forename: K J
authors.initials: KJ
authors.lastname: Helzlsouer
mesh_terms: D000328:Adult; D000368:Aged; D001205:Ascorbic Acid / Q000097:blood*; D001793:Blood Preservation; D002338:Carotenoids / Q000097:blood*; D015925:Cryopreservation; D004355:Drug Stability; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000737:chemistry*; D012189:Retrospective Studies; D013997:Time Factors; D014801:Vitamin A / Q000097:blood*; D014810:Vitamin E / Q000097:blood*
chemical_list: D014801:Vitamin A; D014810:Vitamin E; D002338:Carotenoids; D001205:Ascorbic Acid