Retinoic acid and methotrexate specifically increase PHA-E-lectin binding to a 67-kDa glycoprotein in LA-N-1 human neuroblastoma cells.
Retinoic acid (RA) decreased growth and increased morphologic differentiation of human neuroblastoma LA-N-1 cells. These phenomena correlated with a specific enhancement of PHA-E lectin binding to a glycoprotein of MW 67 kDa (gp67). Gp67 was found susceptible to N-glycanase and displayed BSA binding by affinity chromatography analysis. The chemotherapeutic agent methotrexate (MTX) also reduced growth and induced differentiation of LA-N-1 cells. In addition, the cells responded to MTX as well as to doxorubicin by a marked increase in PHA-E binding to gp67. We conclude that reduced growth and induction of morphological differentiation of LA-N-1 cells correlates with increased binding of PHA-E to gp67.
Ross
S A
SA
Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bethesda, MD 20892, USA.
Jones
C S
CS
De Luca
L M
LM
D000581:Amidohydrolases / Q000494:pharmacology; D000906:Antibodies; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002846:Chromatography, Affinity; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008727:Methotrexate / Q000494:pharmacology*; D009363:Neoplasm Proteins / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism* / Q000473:pathology; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D010835:Phytohemagglutinins / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D012680:Sensitivity and Specificity; D012685:Sepharose; D012710:Serum Albumin, Bovine / Q000276:immunology; D013194:Staining and Labeling / Q000379:methods; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000906:Antibodies; D037102:Lectins; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D010835:Phytohemagglutinins; C067494:erythroagglutinating phytohemagglutinin; D012710:Serum Albumin, Bovine; D014212:Tretinoin; D012685:Sepharose; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D008727:Methotrexate