A bioassay for bovine interleukin-1 by the A375 cell growth inhibition.
Usefulness of a human melanoma cell line A375 was evaluated for detecting bovine interleukin-1 (IL-1). The A375 cell growth was inhibited by culture supernatant of lipopolysaccharide-stimulated bovine peripheral blood mononuclear cells (LPSsup) in a dose dependent manner. A mixture of anti-human IL-1 alpha and beta antibodies suppressed 60% of this inhibitory activity and was confirmed to bind to about 23 k dalton peptides in the LPSsup by Western blotting. Although serum and bronchoalveolar lavage fluid from a healthy cow showed a low inhibitory activity, those from pneumonic cows showed the higher activities. These activities were also suppressed by anti-human IL-1 antibodies. These findings show the A375 cell growth inhibition assay can be a useful bioassay for bovine IL-1 (like) activity.
Goto
M
M
Department of Veterinary Microbiology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Japan.
Kirisawa
R
R
Tajima
M
M
Takahashi
K
K
Iwai
H
H
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001681:Biological Assay; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000494:pharmacology; D008545:Melanoma; D051379:Mice; D011014:Pneumonia / Q000276:immunology / Q000662:veterinary; D011994:Recombinant Proteins / Q000032:analysis / Q000494:pharmacology; D012016:Reference Values; D014407:Tumor Cells, Cultured
D000906:Antibodies; D007375:Interleukin-1; D011994:Recombinant Proteins