Determination of hexokinase isoenzyme I and II composition by RT-PCR: increased hexokinase isoenzyme II in human renal cell carcinoma.
Hexokinase isoenzyme composition has been noted to vary in different tissues and with the developmental and metabolic status of the cell. Until now these investigations were performed either by isoenzyme electrophoresis or by column chromatography. In this report we described an RNA-PCR method to evaluate the percentage of HK-1 and HK-2 in different rat tissues. Furthermore we applied the method to determine if a shift in isoform composition is detectable in human renal carcinomas compared to normal kidney tissue. In all of our specimens we were able to detect a shift toward HK-2 in the carcinoma specimens. We discuss a possible role for the detection of the shift in isoenzyme composition as a possible marker to discriminate between normal and malignant specimens.
Adams
V
V
Department of Pathology, University of Zürich, Switzerland.
Kempf
W
W
Hassam
S
S
Briner
J
J
D000328:Adult; D000818:Animals; D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000201:enzymology*; D017931:DNA Primers; D006593:Hexokinase / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000032:analysis* / Q000235:genetics; D007668:Kidney / Q000201:enzymology; D007680:Kidney Neoplasms / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012194:RNA-Directed DNA Polymerase; D051381:Rats
D017931:DNA Primers; D007527:Isoenzymes; D012333:RNA, Messenger; D006593:Hexokinase; D012194:RNA-Directed DNA Polymerase