title: Effects of hypothermia on the survival and cryopreservation of minipig ileal cells and Chinese hamster ovary cells.
abstract: Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25 degrees C and 3 hours recovery at 37 degrees C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of 2 immortal pig ileal cell lines even though epithelioid IPI-2I cells were more tolerant to hypothermia than IPI-1 fibroblasts. Epithelioid cells survived 3 days at 25 degrees C with unaltered expression of cytokeratin-18 whereas colonies of fibroblasts did not survive more than a day at 25 degrees C (p < 0.001). These results suggest that hypothermia-tolerance of pig ileal cell lines might differ according to cell lineage calling for further experiments on small intestinal primary cell culture.
authors.affiliation: INRA, Laboratoire de Pathologie Infectieuse et Immunologie, Nouzilly, France.
authors.forename: B
authors.initials: B
authors.lastname: Kaeffer
authors.forename: I G
authors.initials: IG
authors.lastname: Uriel
authors.forename: E
authors.initials: E
authors.lastname: Bottreau
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis; D002461:Cell Line, Transformed; D002470:Cell Survival; D003080:Cold Temperature; D006224:Cricetinae; D003412:Cricetulus / Q000033:anatomy & histology*; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000494:pharmacology; D046508:Culture Techniques / Q000379:methods*; D004847:Epithelial Cells; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D007082:Ileum / Q000166:cytology*; D007413:Intestinal Mucosa / Q000166:cytology; D007633:Keratins / Q000096:biosynthesis; D009499:Neutral Red; D010053:Ovary / Q000166:cytology*; D013539:Simian virus 40 / Q000235:genetics; D013045:Species Specificity; D013552:Swine; D013556:Swine, Miniature / Q000033:anatomy & histology*
chemical_list: D000952:Antigens, Polyomavirus Transforming; D003451:Cryoprotective Agents; D009499:Neutral Red; D007633:Keratins