title: Thermus silvanus sp. nov. and Thermus chliarophilus sp. nov., two new species related to thermus ruber but with lower growth temperatures.
abstract: Strains of Thermus silvanus sp. nov. and strains of Thermus chliarophilus sp. nov. were isolated from the hot spring at Vizela in northern Portugal and the hot spring at Alcafache in central Portugal, respectively. The strains of T. silvanus produce orange-red-pigmented colonies and have an optimum growth temperature of about 55 degrees C, while the strains of T. chliarophilus produce yellow-pigmented colonies and have an optimum growth temperature of about 50 degrees C. The strains of both species are catalase negative. These species can be distinguished from each other and from Thermus ruber by biochemical characteristics, fatty acid composition data, and 16S rRNA gene sequence data. Our phylogenetic analysis showed that strains VI-R2T (T = type strain) and ALT-8T belong to the T. ruber line of descent. The type strain of T. silvanus is strain VI-R2 (= DSM 9946), and the type strain of T. chliarophilus is strain ALT-8 (= DSM 9957).
authors.affiliation: Departamento de Bioquímica, Universidade de Coimbra, Portugal.
authors.forename: S
authors.initials: S
authors.lastname: Tenreiro
authors.forename: M F
authors.initials: MF
authors.lastname: Nobre
authors.forename: M S
authors.initials: MS
authors.lastname: da Costa
mesh_terms: D001483:Base Sequence; D004275:DNA, Ribosomal / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013824:Thermus / Q000145:classification* / Q000235:genetics / Q000254:growth & development
chemical_list: D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S