"Pseudoclusters" and typing by random amplification of polymorphic DNA of Aspergillus fumigatus.
This short report serves as a warning to the unwary of possible "pseudoclusters" of infection with Aspergillus fumigatus as shown by the typing system, random amplification of polymorphic DNA (RAPD). This was demonstrated by typing 10 epidemiologically distinct isolates of A fumigatus using two different preparations of Taq DNA polymerase. One of the enzymes did not discriminate between the isolates, giving the false impression that a cluster of infection had occurred. Enzyme source is thus a key variable when using RAPD to distinguish between isolates of A fumigatus.
Loudon
K W
KW
Department of Medical Microbiology, University of Manchester Medical School.
Coke
A P
AP
Burnie
J P
JP
D001232:Aspergillus fumigatus / Q000145:classification*; D001483:Base Sequence; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis*; D004259:DNA-Directed DNA Polymerase; D005784:Gene Amplification; D006801:Humans; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D012194:RNA-Directed DNA Polymerase; D019914:Taq Polymerase
D017931:DNA Primers; D004271:DNA, Fungal; D019914:Taq Polymerase; D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase