Cross-linking of CD30 induces HIV expression in chronically infected T cells.
CD30, a member of the tumor necrosis factor (TNF) receptor family, is expressed constitutively on the surface of the human T cell line ACH-2, which is chronically infected with human immunodeficiency virus type-1 (HIV)-1. We demonstrate that cross-linking CD30 with an anti-CD30-specific monoclonal antibody, which mimics the described biological activities of the CD30 ligand (CD30L), results in HIV expression. CD30 cross-linking does not alter proliferation of ACH-2 cells and the induction of HIV expression is not mediated by endogenous TNF alpha/beta. Furthermore, cross-linking of CD30 leads to NF-kappa B activation and enhanced HIV transcription. Thus, CD30-CD30L interactions mediate the induction of HIV expression by a kappa B-dependent pathway that is independent of TNF. This mechanism may be important in the activation of HIV expression from latently infected CD4+ T cells, especially in lymphoid organs where cell to cell contact is conducive to receptor-ligand interactions.
Biswas
P
P
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-2520, USA.
Smith
C A
CA
Goletti
D
D
Hardy
E C
EC
Jackson
R W
RW
Fauci
A S
AS
D000911:Antibodies, Monoclonal / Q000276:immunology; D015152:Blotting, Northern; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis; D003429:Cross Reactions / Q000276:immunology; D006678:HIV / Q000254:growth & development*; D006801:Humans; D016130:Immunophenotyping; D017730:Ki-1 Antigen / Q000276:immunology*; D008213:Lymphocyte Activation; D016328:NF-kappa B / Q000096:biosynthesis; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology* / Q000821:virology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014775:Virus Activation / Q000276:immunology*
D000911:Antibodies, Monoclonal; D017730:Ki-1 Antigen; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D015500:Chloramphenicol O-Acetyltransferase; D012194:RNA-Directed DNA Polymerase