title: Divergence of a flagellin protein in Serratia marcescens.
abstract: A gene (hag) encoding the flagellin (Fla) protein was cloned from Serratia marcescens (Sm) 8000, the wild-type strain of Sr41. The hag gene codes for a 348-amino-acid (aa) protein of 36.7 kDa. The predicted aa sequence showed 79% homology compared with the Fla of Sm 274 which has been reported previously [Harshey et al., Gene 79 (1989) 1-8]. Dot-matrix analysis of the Sm 8000 Fla showed that the N- and C-terminal regions of this protein were highly similar to those of other bacterial Fla. However, the aa sequence of the middle portion was quite different from that of a variant strain of the same species, Sm 274.
authors.affiliation: Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Akatsuka
authors.forename: E
authors.initials: E
authors.lastname: Kawai
authors.forename: K
authors.initials: K
authors.lastname: Omori
authors.forename: T
authors.initials: T
authors.lastname: Shibatani
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D005408:Flagellin / Q000737:chemistry / Q000235:genetics*; D005798:Genes, Bacterial; D014644:Genetic Variation; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D012706:Serratia marcescens / Q000235:genetics*
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins; D005408:Flagellin