Cloning of the two pyruvate kinase isoenzyme structural genes from Escherichia coli: the relative roles of these enzymes in pyruvate biosynthesis.
We report the cloning of the pykA and pykF genes from Escherichia coli, which code for the two pyruvate kinase isoenzymes (ATP:pyruvate 2-O-phosphotransferases; EC 2.7.1.40) in this microorganism. These genes were insertionally inactivated with antibiotic resistance markers and utilized to interrupt one or both pyk genes in the E. coli chromosome. With these constructions, we were able to study the role of these isoenzymes in pyruvate biosynthesis.
Ponce
E
E
Departamento de Microbiología Molecular, Universidad Nacional Autónoma de México (UNAM), Cuernavaca, Morelos.
Flores
N
N
Martinez
A
A
Valle
F
F
Bolívar
F
F
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005798:Genes, Bacterial / Q000235:genetics*; D005942:Gluconates / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D011770:Pyruvate Kinase / Q000235:genetics* / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism*; D019289:Pyruvic Acid; D012266:Ribose / Q000378:metabolism; D017422:Sequence Analysis, DNA
D005942:Gluconates; D007527:Isoenzymes; D011773:Pyruvates; D012266:Ribose; D019289:Pyruvic Acid; D011770:Pyruvate Kinase; D005947:Glucose; C030691:gluconic acid