title: Detection of mip-like sequences and Mip-related proteins within the family Rickettsiaceae.
abstract: The Mip surface protein, a prokaryotic analog of the FK506-binding proteins, enhances the ability of Legionella pneumophila to infect macrophages and protozoa. Using mip-specific probes and low-stringency Southern hybridizations, we have detected DNA sequences homologous to mip within Coxiella burnetii and Rochalimaea quintana. Using specific anti-Mip antisera and immunoblot analysis, we also detected Mip-related proteins within these bacteria as well as within Rickettsia and Ehrlichia species. These data suggest that Mip-related proteins have broad significance for host-parasite interactions. However, they also indicate that care must be exercised when using mip probes or anti-Mip antibodies for the detection of Legionella organisms in water or clinical samples.
authors.affiliation: Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois 60611.
authors.forename: N P
authors.initials: NP
authors.lastname: Cianciotto
authors.forename: W
authors.initials: W
authors.lastname: O'Connell
authors.forename: G A
authors.initials: GA
authors.lastname: Dasch
authors.forename: L P
authors.initials: LP
authors.lastname: Mallavia
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics*; D018413:Bartonella quintana / Q000235:genetics; D016997:Coxiella burnetii / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004536:Ehrlichia / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D020104:Immunophilins; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics*; D008969:Molecular Sequence Data; D019696:Peptidylprolyl Isomerase; D012281:Rickettsia / Q000235:genetics; D012287:Rickettsiaceae / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins; D020104:Immunophilins; C512922:Mip protein, Legionella pneumophila; D019696:Peptidylprolyl Isomerase