title: Staphylococcus aureus expresses a major histocompatibility complex class II analog.
abstract: Staphylococcus aureus expresses various surface proteins which specifically recognize and bind to different host molecules. We have previously identified a bacterial protein that exhibits a broad specificity and binds to several mammalian extracellular proteins. The gene encoding this bacterial component has now been cloned and sequenced. The deduced protein consists predominantly of six repeated domains of 110 residues. Each of the repeated domains contain a subdomain of 31 residues that share striking sequence homology with a segment in the peptide binding groove of the beta chain of the major histocompatibility complex (MHC) class II proteins from different mammalian species. The purified recombinant bacterial protein bound several mammalian proteins, including recombinant osteopontin, suggesting a protein-protein interaction and also specifically recognized a 15-amino acid residue synthetic peptide. Taken together, these results suggest that the bacterial protein resembles mammalian MHC class II molecules with respect to both sequence similarities and peptide binding capabilities.
authors.affiliation: Center for Extracellular Matrix Biology, Texas A & M University, Houston 77030-3303, USA.
authors.forename: K
authors.initials: K
authors.lastname: Jönsson
authors.forename: D
authors.initials: D
authors.lastname: McDevitt
authors.forename: M H
authors.initials: MH
authors.lastname: McGavin
authors.forename: J M
authors.initials: JM
authors.lastname: Patti
authors.forename: M
authors.initials: M
authors.lastname: Höök
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli; D006023:Glycoproteins / Q000737:chemistry; D006684:HLA-DR Antigens / Q000737:chemistry*; D000882:Haplorhini; D006801:Humans; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D053495:Osteopontin; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D012795:Sialoglycoproteins / Q000737:chemistry; D013211:Staphylococcus aureus / Q000378:metabolism*; D019096:Vitronectin
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D006023:Glycoproteins; D006684:HLA-DR Antigens; C095564:Map protein, Staphylococcus aureus; D011994:Recombinant Proteins; C506895:SPP1 protein, human; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; D019096:Vitronectin; D053495:Osteopontin