title: Epithelial cell proliferation in odontogenic keratocysts: a comparative immunocytochemical study of Ki67 in simple, recurrent and basal cell naevus syndrome (BCNS)-associated lesions.
abstract: The aim of the present study was to investigate epithelial cell proliferation in the linings of odontogenic cysts, including three different subtypes of odontogenic keratocyst (OKC), namely simple (non-recurrent), recurrent and basal cell naevus syndrome (BCNS)-associated lesions. Ki67 immunoreactivity in OKC (simple, n = 10; recurrent, n = 8; syndrome, n = 9), dentigerous cysts (DC, n = 5), radicular cysts (RC, n = 5) and normal oral mucosa (n = 7) was studied using a biotin-streptavidin-peroxidase method on paraffin sections after microwave treatment. Ki67+ epithelial cells were counted manually and related to the length of basement membrane (BM) as determined by TV image analysis. Data were analysed by the Mann-Whitney U test. The number of Ki67+ cells in simple OKC linings (53.1 +/- 17.8 cells/mmBM) was similar to that in oral epithelium (42.5 +/- 12.7 cells/mmBM; P > 0.2). However, both contained significantly more Ki67+ cells than DC (3.9 +/- 1.3 cells/mmBM) and RC linings (6.7 +/- 4.8 cells/mmBM; P < 0.006). The epithelial distribution of Ki67+ cells differed between groups, with the percentage of positive cells in basal layers in OKC linings (7.0 +/- 2.1%) being significantly lower than that of oral epithelia (35.9 +/- 5.6%), DC (78.4 +/- 8.4%) and RC (80.6 +/- 7.7%) linings respectively (P < 0.003). Comparison of Ki67 expression within the OKC group revealed no significant difference between simple and recurrent lesions (44.0 +/- 13.8 cells/mmBM; P > 0.3). However, OKC associated with BCNS contained significantly higher numbers of Ki67+ cells (91.8 +/- 35.6 cells/mmBM; P < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Unit of Oral Pathology, School of Dentistry, University of Birmingham, England.
authors.forename: T J
authors.initials: TJ
authors.lastname: Li
authors.forename: R M
authors.initials: RM
authors.lastname: Browne
authors.forename: J B
authors.initials: JB
authors.lastname: Matthews
mesh_terms: D000704:Analysis of Variance; D001478:Basal Cell Nevus Syndrome / Q000276:immunology / Q000473:pathology*; D015415:Biomarkers; D018797:Cell Cycle Proteins; D002455:Cell Division; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins; D019394:Ki-67 Antigen; D008872:Microwaves; D009062:Mouth Neoplasms / Q000276:immunology / Q000473:pathology; D009363:Neoplasm Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D009807:Odontogenic Cysts / Q000276:immunology / Q000473:pathology*; D016612:Paraffin Embedding; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012008:Recurrence; D016196:S Phase / Q000502:physiology; D018709:Statistics, Nonparametric
chemical_list: D015415:Biomarkers; D018797:Cell Cycle Proteins; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen; D007633:Keratins