Molecular cloning and transcriptional analysis of the Aspergillus terreus gla1 gene encoding a glucoamylase.
The Aspergillus terreus gla1 gene, coding for a glucoamylase, has been cloned by heterologous hybridization. The gene is interrupted by four introns and encodes a protein with an N-terminal catalytic domain and a C-terminal starch-binding domain. The expression of the gene is induced by starch and maltose and repressed by glucose.
Ventura
L
L
Departamento de Biotecnología, Consejo Superior de Investigaciones Científicas, Valencia, Spain.
González-Candelas
L
L
Pérez-González
J A
JA
Ramón
D
D
D000595:Amino Acid Sequence; D000681:Amylases / Q000235:genetics*; D001230:Aspergillus / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic
D012333:RNA, Messenger; D000681:Amylases