Polyclonal antibodies against NCAM and tenascin delay endplate reinnervation.
Experiments were performed to block molecules with antibodies which are upregulated in nerve and muscle following denervation. The delay in endplate reinnervation was taken as a measure for their involvement in regeneration. Gluteus maximus muscles of 86 male CBA/J mice were hemidenervated by freezing the caudal gluteal nerve at a defined position. The degree of reinnervation was evaluated in identified endplates by repeated vital staining of ACh receptors with rhodaminated alpha-bungarotoxin and of axons with 4Di-2ASP. Normally, endplates were completely reinnervated by 13-14 days (108 endplates in seven muscles). After daily application of polyclonal antibodies against NCAM or tenascin, reinnervation was significantly delayed. Preimmune serum, rabbit immunoglobulins or saline did not show this effect. Several monoclonal antibodies against NCAM (H-28) and tenascin (576, 578, 630, 633) showed a tendency but no significant effect. It is concluded that both NCAM and tenascin, upregulated after denervation, are involved in axon guidance and/or endplate reinnervation.
Langenfeld-Oster
B
B
Department of Physiology (Neurophysiology), University of Bonn, Germany.
Faissner
A
A
Irintchev
A
A
Wernig
A
A
D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015415:Biomarkers; D015816:Cell Adhesion Molecules, Neuronal / Q000276:immunology / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000276:immunology / Q000502:physiology*; D005456:Fluorescent Dyes; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009045:Motor Endplate / Q000166:cytology / Q000502:physiology*; D009121:Muscle Denervation; D018482:Muscle, Skeletal / Q000294:innervation; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000502:physiology; D011950:Receptors, Cholinergic / Q000032:analysis / Q000378:metabolism; D013569:Synapses / Q000502:physiology; D019063:Tenascin; D013997:Time Factors
D000906:Antibodies; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D005456:Fluorescent Dyes; D009419:Nerve Tissue Proteins; D011950:Receptors, Cholinergic; D019063:Tenascin