title: Relationship between different isoforms of nitric oxide synthase and cyclooxygenase in various cell types.
abstract: Nitric oxide (NO) and prostacyclin (PGI2), formed by NO synthase (NOS) and cyclooxygenase (COX), respectively, are two potent anti-aggregatory vasodilators released from endothelial cells. Both NOS and COX exist as constitutive and inducible isoforms. We have shown that NOS and COX are co-induced in vitro and in vivo by bacterial endotoxin and that low amounts of NO increase whereas high amounts inhibit the activity and expression of inducible COX in vitro.
authors.affiliation: William Harvey Research Institute, St. Bartholomew's Hospital, Medical College, U.K.
authors.forename: T A
authors.initials: TA
authors.lastname: Swierkosz
authors.forename: J A
authors.initials: JA
authors.lastname: Mitchell
authors.forename: A
authors.initials: A
authors.lastname: Tomlinson
authors.forename: R M
authors.initials: RM
authors.lastname: Botting
authors.forename: T D
authors.initials: TD
authors.lastname: Warner
authors.forename: J R
authors.initials: JR
authors.lastname: Vane
mesh_terms: D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D002460:Cell Line; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D004731:Endotoxins / Q000633:toxicity; D004790:Enzyme Induction / Q000187:drug effects; D011464:Epoprostenol / Q000096:biosynthesis; D007150:Immunohistochemistry; D007213:Indomethacin / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000201:enzymology; D008297:Male; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D019001:Nitric Oxide Synthase; D009599:Nitroprusside / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000201:enzymology; D019323:omega-N-Methylarginine
chemical_list: D004731:Endotoxins; D009599:Nitroprusside; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; D015121:6-Ketoprostaglandin F1 alpha; D001120:Arginine; D011464:Epoprostenol; D019001:Nitric Oxide Synthase; D011451:Prostaglandin-Endoperoxide Synthases; D000594:Amino Acid Oxidoreductases; D007213:Indomethacin