Biotinylated L-selectin ligand analogs as cytochemical probes in cytospin preparations.
Amino-derivatives of L-selectin ligand analogs: phosphonoester core polysaccharide (PPME) and fucoidin were biotinylated with the use of biotinyl-N-succinimide ester, and these biotinylated analogs b-PPME and b-fucoidin were demonstrated as useful tools to investigate the functional activity of L-selectins in cytospin preparations obtained from healthy human donors and from patients with chronic lymphocytic leukemia (CLL). The avidin/biotin system adds a new alternative to the application of the L-selectin ligand analogs (PPME, fucoidin) which have been formerly used as fluoresceinated, solid phase or immobilized probes.
Vass
J A
JA
Department of Pathology, University Medical School of Pécs, Hungary.
Csanaky
G
G
D001710:Biotin; D002498:Centrifugation; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D007202:Indicators and Reagents; D019041:L-Selectin / Q000032:analysis*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology; D008214:Lymphocytes / Q000737:chemistry* / Q000378:metabolism; D008351:Mannans / Q000378:metabolism; D008360:Mannosephosphates / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D013113:Spin Labels
D007202:Indicators and Reagents; D008351:Mannans; D008360:Mannosephosphates; D011134:Polysaccharides; D013113:Spin Labels; D019041:L-Selectin; C065532:mannose-6-phosphate rich phosphomannan; D001710:Biotin; C007789:fucoidan