In vitro purging with BCR-ABL antisense oligodeoxynucleotides does not prevent haematologic reconstitution after autologous bone marrow transplantation.
We treated a patient with chronic myeloid leukaemia in accelerated phase with autologous bone marrow transplantation. Before reinfusion, cells were purged in vitro with a 26-mer phosphorothioate antisense oligodeoxynucleotide specific for the B2A2 junction. Incubation with antisense oligodeoxynucleotides produced a 24 and 41% reduction of CFU-GM and CD34+ cells, respectively. However, an in vitro test previously performed as a screening for patient inclusion in this procedure, revealed a 38 and 75% reduction of colony formation after 24-h and 168-h incubation, respectively. The patient showed bone marrow engraftment 15 days after reinfusion and haematological reconstitution after 17 and 25 days for platelets and neutrophils, respectively. Using fluorescence in situ hybridization in interphase nuclei, we demonstrated the presence of a proportion of Ph-negative cells in repeated controls after the autograft. The patient is now in unmaintained complete haematological remission 9 months after the autograft.
de Fabritis
P
P
Department of Human Biopathology, University 'La Sapienza' Rome, Italy.
Amadori
S
S
Petti
M C
MC
Mancini
M
M
Montefusco
E
E
Picardi
A
A
Geiser
T
T
Campbell
K
K
Calabretta
B
B
Mandelli
F
F
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D016465:Bone Marrow Purging / Q000379:methods*; D016026:Bone Marrow Transplantation / Q000379:methods*; D006410:Hematopoiesis; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000628:therapy*; D016376:Oligonucleotides, Antisense / Q000737:chemistry; D014182:Transplantation, Autologous
D015703:Antigens, CD; D018952:Antigens, CD34; D016376:Oligonucleotides, Antisense