title: 1-Nitropyrene as a marker for the mutagenicity of diesel exhaust-derived particulate matter in workplace atmospheres.
abstract: The use of 1-nitropyrene (1-NP) as a marker for the occupational exposure to diesel exhaust (DE) mutagens was investigated in workplace atmospheres contaminated with DE from a variety of emission sources, such as power supplies, forklifts, trucks, caterpillar vehicles, trains, ships' engines, and vehicles in city traffic. Total suspended particulate matter was collected by area sampling. The 1-NP content of acetone extracts of these samples as determined by gas chromatography-high resolution mass spectrometry varied from 0.080 to 17 micrograms/g acetone extractable matter, corresponding to air concentrations of 0.012 to 1.2 ng/m3. A sample collected in a rural area contained 0.0017 ng/m3 1-NP. The mutagenicity of the extracts was tested in the Salmonella typhimurium strains TA98 and TA1538, using the microsuspension assay with and without metabolic activation by an exogeneous metabolizing system (rat liver S9-fraction). In addition, the S. typhimurium strains YG1021 and YG1024 were used because of their high sensitivity towards the mutagenicity of nitro polycyclic aromatic hydrocarbons. When plotting the mutagenic potency of the air sample extracts as determined in the absence of liver S9 versus the particle-associated 1-NP level, a relatively high correlation (r = 0.80-0.91) was observed in all of the S. typhimurium strains. High correlations (r = 0.80-0.93) were also observed when plotting the results of mutagenicity testing after activation by S9 versus the outcome of chemical analysis. These results show that the 1-NP content of workplace air samples is associated with their mutagenic potency, suggesting that 1-NP may be used as a marker for occupational exposure to DE-derived particle-associated mutagens.
authors.affiliation: Toxicology Department, University of Nijmegen, The Netherlands.
authors.forename: P T
authors.initials: PT
authors.lastname: Scheepers
authors.forename: M H
authors.initials: MH
authors.lastname: Martens
authors.forename: D D
authors.initials: DD
authors.lastname: Velders
authors.forename: P
authors.initials: P
authors.lastname: Fijneman
authors.forename: M
authors.initials: M
authors.lastname: van Kerkhoven
authors.forename: J
authors.initials: J
authors.lastname: Noordhoek
authors.forename: R P
authors.initials: RP
authors.lastname: Bos
mesh_terms: D000395:Air Pollutants, Occupational / Q000032:analysis* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000379:methods*; D006801:Humans; D016014:Linear Models; D009152:Mutagenicity Tests; D016273:Occupational Exposure; D011083:Polycyclic Compounds / Q000032:analysis; D011721:Pyrenes / Q000032:analysis*; D012486:Salmonella typhimurium / Q000187:drug effects; D001335:Vehicle Emissions / Q000032:analysis / Q000633:toxicity*
chemical_list: D000395:Air Pollutants, Occupational; D011083:Polycyclic Compounds; D011721:Pyrenes; D001335:Vehicle Emissions; C049029:2-nitropyrene