Proteins reacting with anti-spectrin antibodies are present in Chlamydomonas cells.
It was found either in Western-blot analysis or in indirect immunofluorescence microscopy that cells of the alga Chlamydomonas reinhardtii contain polypeptides cross-reacting with antibodies directed against red blood cell spectrin. The protein could also be detected by immunoprecipitation with anti-spectrin antibodies. C. reinhardtii cells contain distinct polypeptide chains reacting with antibodies directed against either alpha- or beta-spectrin subunits. This protein was extracted from the cells with low ionic strength solution but was not with nonionic detergent.
Lorenz
M
M
University of Wroclaw, Institute of Biochemistry, Poland.
Bisikirska
B
B
Hanus-Lorenz
B
B
Strzalka
K
K
Sikorski
A F
AF
D000818:Animals; D000906:Antibodies; D002417:Cattle; D002457:Cell Extracts; D016825:Chlamydomonas reinhardtii / Q000737:chemistry*; D003429:Cross Reactions; D000939:Epitopes / Q000032:analysis; D019084:Fluorescent Antibody Technique, Indirect; D008970:Molecular Weight; D011233:Precipitin Tests; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D013049:Spectrin / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification
D000906:Antibodies; D002457:Cell Extracts; D000939:Epitopes; D011506:Proteins; D013049:Spectrin