Effects of kainic acid induced seizures on immediate early gene expression in mice with a targeted mutation of the CREB gene.
The present study examined the response of immediate early genes following kainic acid induced seizures in mice lacking the alpha and delta isoforms of CREB. mRNA levels for c-fos, c-jun, and Krox-24 were measured following limbic seizure activity and were found to be induced in wild type as well as CREB mutant mice. This effect was also seen for these three mRNAs at the protein level as well as for FOS-B. Furthermore the time course of expression of FOS, JUN, KROX-24, and FOS-B proteins were essentially the same in CREB mutant mice as compared to wild-type controls. These data suggest that CREB alpha and delta are not required for the induction of immediate early genes following pharmacologically induced seizures.
Blendy
J A
JA
Division Molecular Biology of the Cell I, German Cancer Research Center, Heidelberg.
Schmid
W
W
Kiessling
M
M
Schütz
G
G
Gass
P
P
D051698:Activating Transcription Factor 2; D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D017781:Genes, Immediate-Early; D016762:Genes, fos / Q000187:drug effects; D016758:Genes, jun / Q000187:drug effects; D007150:Immunohistochemistry; D007608:Kainic Acid / Q000494:pharmacology*; D008032:Limbic System / Q000201:enzymology / Q000502:physiology; D051379:Mice; D016297:Mutagenesis, Site-Directed / Q000187:drug effects*; D012313:RNA / Q000096:biosynthesis; D012640:Seizures / Q000139:chemically induced* / Q000201:enzymology / Q000503:physiopathology; D014157:Transcription Factors
D051698:Activating Transcription Factor 2; D017362:Cyclic AMP Response Element-Binding Protein; D014157:Transcription Factors; D012313:RNA; D007608:Kainic Acid