title: Antifungal compounds induced in the dual culture with Phytolacca americana callus and Botrytis fabae.
abstract: In order to investigate new metabolites which are only induced in a plant callus infected by a pathogenic fungus, dual cultures with combinations of 10 species of fungi and 6 plant cell lines from different species were established. Among the combinations tested, the methanolic extract of a dual culture consisting of a plant cell line, Phytolacca americana and a fungus, Botrytis fabae showed a marked antifungal activity to Cladosporium herbarum. The main active constituent of this extract was identified to be phytolaccoside B (Pls B) by the spectroscopic analyses.
authors.affiliation: Department of Agricultural Science, Faculty of Agriculture, Okayama University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Kobayashi
authors.forename: K
authors.initials: K
authors.lastname: Hagihara
authors.forename: S
authors.initials: S
authors.lastname: Kajiyama
authors.forename: H
authors.initials: H
authors.lastname: Kanzaki
authors.forename: K
authors.initials: K
authors.lastname: Kawazu
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002963:Cladosporium / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D003904:Mitosporic Fungi / Q000472:pathogenicity* / Q000502:physiology; D010944:Plants / Q000378:metabolism / Q000382:microbiology*; D013045:Species Specificity
chemical_list: D000935:Antifungal Agents