Identification of Campylobacter fetus by PCR-DNA probe method.
A PCR method for rapid identification of Campylobacter fetus subsp. fetus was evaluated. A fragment of the gene coding for 16S rRNA was amplified from crude cell lysates of 18 C. fetus strains and 30 strains representing other Campylobacter species and subspecies. The amplicons were probed by dot blot hybridization with a digoxigenin-labeled C. fetus-specific oligonucleotide probe. The probe reacted only with C. fetus subsp. fetus and C. fetus subsp. venerealis and may be useful for rapid identification in clinical laboratories.
Blom
K
K
Foodborne and Diarrheal Diseases Branch, National Center for Infectious Diseases, Atlanta, Georgia 30333, USA.
Patton
C M
CM
Nicholson
M A
MA
Swaminathan
B
B
D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D001483:Base Sequence; D002169:Campylobacter Infections / Q000175:diagnosis / Q000382:microbiology; D002168:Campylobacter fetus / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity
D017931:DNA Primers; D015342:DNA Probes; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S