Effects of interleukin-4 or stem cell factor on mast cell mediator release and cytokine gene expression.
We have investigated the capacity of interleukin (IL)-4 or stem cell factor (SCF) to induce direct mediator release from rodent peritoneal mast cells, and also to induce or regulate cytokine gene expression in the human HMC-1 mast cell line. SCF, but not IL-4, induced low levels of serotonin release from mouse or rat peritoneal mast cells; rat mast cells acquired enhanced responsiveness to SCF during culture. IL-4, but not SCF, enhanced ionomycin-induced transcription and secretion of several genes, including the cytokines IL-3, IL-4, granulocyte/macrophage-colony-stimulating factor, IL-8 and the receptor for IL-6 in the human HMC-1 mast cell line.
Coleman
J W
JW
Department of Pharmacology and Therapeutics, University of Liverpool, UK.
Buckley
M G
MG
Taylor
A M
AM
Banks
E M
EM
Williams
C M
CM
Holliday
M R
MR
Thompson
J
J
D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation / Q000187:drug effects*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis* / Q000235:genetics; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D007378:Interleukins / Q000096:biosynthesis* / Q000235:genetics; D015759:Ionomycin / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D018123:Receptors, Interleukin / Q000096:biosynthesis* / Q000235:genetics; D019947:Receptors, Interleukin-6; D012701:Serotonin / Q000378:metabolism; D019089:Stem Cell Factor
D016298:Hematopoietic Cell Growth Factors; D007378:Interleukins; D018123:Receptors, Interleukin; D019947:Receptors, Interleukin-6; D019089:Stem Cell Factor; D015847:Interleukin-4; D012701:Serotonin; D015759:Ionomycin; D016178:Granulocyte-Macrophage Colony-Stimulating Factor