title: Aminoacylation of transfer RNAs with 2-thiouridine derivatives in the wobble position of the anticodon.
abstract: The first position or 'wobble base' in the anticodon of tRNAs is frequently the site of post-transcriptional modification. In Escherichia coli, glutamine, glutamate, and lysine tRNAs contain 2-thiouridine derivatives in this position, and the significance of these modifications has been under investigation since their discovery. Here we describe the investigations to link 2-thiouridine derivatives to aminoacylation of these tRNAs. The implications of these findings on the evolution of specificity of aminoacyl-tRNA synthetases and on translational regulation are also discussed.
authors.affiliation: Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA.
authors.forename: K C
authors.initials: KC
authors.lastname: Rogers
authors.forename: A T
authors.initials: AT
authors.lastname: Crescenzo
authors.forename: D
authors.initials: D
authors.lastname: Söll
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D000926:Anticodon; D001483:Base Sequence; D005075:Biological Evolution; D004926:Escherichia coli / Q000737:chemistry; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000737:chemistry / Q000378:metabolism*; D012351:RNA, Transfer, Gln / Q000378:metabolism; D012352:RNA, Transfer, Glu / Q000378:metabolism; D012357:RNA, Transfer, Lys / Q000378:metabolism; D013379:Substrate Specificity; D013891:Thiouridine / Q000031:analogs & derivatives* / Q000378:metabolism*
chemical_list: D000926:Anticodon; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D012351:RNA, Transfer, Gln; D012352:RNA, Transfer, Glu; D012357:RNA, Transfer, Lys; D013891:Thiouridine; D000604:Amino Acyl-tRNA Synthetases