Induction of diaphorase-1 by dicoumarol in Drosophila virilis larvae.
Drosophila diaphorase-1 (DIA-1) is an enzyme similar to mammalian DT-diaphorase and is inhibited in vitro by dicoumarol. However, a ten-fold increase in DIA-1 activity was observed when third instar Drosophila virilis larvae were fed on a diet containing 0.1 M dicoumarol for 48 h. This induction was shown to be dose dependent and immunoprecipitation experiments with DIA-1 anti-serum demonstrated an increase in the DIA-1 protein level in dicoumarol-treated larvae. The induction of DIA1 by dicoumarol was found to be blocked by actinomycin D, which suggests a transcriptional mechanism of regulation. The opposite effect of dicoumarol on DIA-1 in vitro vs. in vivo suggests that a metabolic conversion takes place after the ingestion of this compound by D. virilis larvae.
Georgieva
T G
TG
Department of Genetics, Faculty of Biology, Sofia University, St. Kliment Ohridski, Bulgaria.
Jankulova
E D
ED
Ralchev
K H
KH
Dunkov
B C
BC
D000818:Animals; D003609:Dactinomycin / Q000494:pharmacology; D001728:Dicumarol / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004330:Drosophila / Q000187:drug effects / Q000201:enzymology*; D004592:Electrophoresis, Starch Gel; D004790:Enzyme Induction; D007814:Larva / Q000187:drug effects / Q000201:enzymology; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000096:biosynthesis* / Q000187:drug effects; D015431:Weight Loss
D003609:Dactinomycin; D001728:Dicumarol; D016660:NAD(P)H Dehydrogenase (Quinone)