Expression of yeast O6-methylguanine-DNA methyltransferase (MGMT) gene.
We cloned the full genomic DNA of yeast (Saccharomyces cerevisiae) O6-methylguanine-DNA methyltransferase (MGMT) gene and examined its expression. The expression of yeast MGMT gene is decreased when cells reach stationary phase and cannot be induced by the pretreatment with alkylating agents, methylmethanesulfonate (MMS) or N-methyl-N'-nitroso-N-nitrosoguanidine (MNNG). The transcription initiation site was determined by primer extension analysis. This analysis showed that the authentic start codon is the ATG at position +32 from transcription initiation site.
Joo
J H
JH
Department of Molecular Biology, Seoul National University, Korea.
Rho
J K
JK
Kim
J H
JH
Kim
W J
WJ
Choe
S Y
SY
Park
S D
SD
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D015966:Gene Expression Regulation, Fungal; D008780:Methyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D019853:O(6)-Methylguanine-DNA Methyltransferase; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development
D008780:Methyltransferases; D019853:O(6)-Methylguanine-DNA Methyltransferase