Differential suppression of axoplasmic transport: effects of light irradiation to the growth cone of cultured dorsal root ganglion neurons.
1. Growth cones of cultured dorsal root ganglion neurons from mice were irradiated using a mercury lamp. 2. The flux of particles of fast retrograde axoplasmic transport decreased promptly after light irradiation without a change in velocity. 3. That of anterograde transport decreased as well, but with a significant latency. The decrease in anterograde flux was attributed to decreased velocity of particles. 4. Video-enhanced contrast microscopy of growth cones revealed transient swelling of growth cones and transient stagnation of particles in growth cones. 5. The longer the neurite, the larger the latency of the change of the anterograde transport; peripheral information was calculated to be conveyed to the cell body at a speed of 6 microns/min. 6. The mechanism of this information conveyance and the export of materials from the cell body are discussed.
Kano
M
M
Laboratory for Photobiology, Institute of Physical and Chemical Research (RIKEN), Sendai, Japan.
Tashiro
H
H
Kawakami
T
T
Takenaka
T
T
Gotoh
H
H
D000818:Animals; D001370:Axonal Transport / Q000502:physiology / Q000528:radiation effects*; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000502:physiology*; D008027:Light; D051379:Mice; D008810:Mice, Inbred C57BL; D016501:Neurites / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D009474:Neurons / Q000502:physiology* / Q000528:radiation effects; D014741:Video Recording