Antibodies that activate beta 2 integrins can generate different ligand binding states.
A human erythroleukemic cell line (K562) that does not normally express beta 2 integrins has been transfected with the genes encoding these integrins. The resulting cell lines show minimal background adhesion but can be stimulated to bind to appropriate substrates when activated with either of two different antibodies to CD18. The two antibodies appear to generate different ligand binding states in LFA-1 such that different members of the ICAM family are recognized. Antibody-activated complement receptor type 3 and p150,95-transfected cells bind protein-coated surfaces, although they require slightly different activation conditions for optimal binding.
Ortlepp
S
S
Celltech Ltd., Slough, GB.
Stephens
P E
PE
Hogg
N
N
Figdor
C G
CG
Robinson
M K
MK
D000911:Antibodies, Monoclonal / Q000276:immunology*; D018821:CD18 Antigens / Q000276:immunology*; D002448:Cell Adhesion / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D016023:Integrins / Q000276:immunology* / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D011951:Receptors, Complement / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014162:Transfection / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D016023:Integrins; D008024:Ligands; D016169:Lymphocyte Function-Associated Antigen-1; D011951:Receptors, Complement; D018799:Intercellular Adhesion Molecule-1; D012710:Serum Albumin, Bovine