title: Tyrosine phosphorylation of vav protooncogene product in primary human myelogenous leukemic cells stimulated by granulocyte colony-stimulating factor.
abstract: Granulocyte colony-stimulating factor(G-CSF), but not granulocyte-macrophage CSF(GM-CSF), induced tyrosine phosphorylation of 95-kDa protein in 13 cases of primary human acute myelogenous leukemic cells. Electrophoretic mobility of 95-kDa phosphoprotein and the protooncogene product p95vav was identical. In addition, p95vav was tyrosine-phosphorylated only in G-CSF-stimulated cells. In contrast to primary leukemic cells, amount of p95vav was under detectable level and G-CSF did not induce tyrosine phosphorylation of 90-100-kDa proteins in human neutrophils. These results indicate specific involvement of vav product in signaling pathway of G-CSF in primary human leukemic cells.
authors.affiliation: Research Institute and Hospital, International Medical Center of Japan, Tokyo.
authors.forename: A
authors.initials: A
authors.lastname: Yuo
authors.forename: S
authors.initials: S
authors.lastname: Kitagawa
authors.forename: S
authors.initials: S
authors.lastname: Iki
authors.forename: M
authors.initials: M
authors.lastname: Yagisawa
authors.forename: E K
authors.initials: EK
authors.lastname: Inuo
authors.forename: T
authors.initials: T
authors.lastname: Mimura
authors.forename: S
authors.initials: S
authors.lastname: Minoda
authors.forename: Y
authors.initials: Y
authors.lastname: Hanazono
authors.forename: H
authors.initials: H
authors.lastname: Hirai
authors.forename: A
authors.initials: A
authors.lastname: Urabe
mesh_terms: D000595:Amino Acid Sequence; D000906:Antibodies; D015153:Blotting, Western; D018797:Cell Cycle Proteins; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D019000:Phosphotyrosine; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000378:metabolism*; D051567:Proto-Oncogene Proteins c-vav; D011519:Proto-Oncogenes / Q000187:drug effects; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism
chemical_list: D000906:Antibodies; D018797:Cell Cycle Proteins; D010455:Peptides; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D051567:Proto-Oncogene Proteins c-vav; D011994:Recombinant Proteins; C496618:VAV1 protein, human; D016179:Granulocyte Colony-Stimulating Factor; D019000:Phosphotyrosine; D014443:Tyrosine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor