Early progression stage of malignancy as revealed by immunohistochemical demonstration of DNA instability; I, Human gastric adenomas.
The degree of DNA-instability as revealed by the immunohistochemical staining with monoclonal anti-single-stranded DNA antibody after acid hydrolysis (DNA-instability test) was used as the marker of malignancy. This was applied to human gastric regenerative epithelium in chronic peptic ulcer (5 cases), adenoma (35 cases), and well differentiated tubular adenocarcinoma (5 cases). Proliferative activity was evaluated by proliferating cell nuclear antigen (PCNA) immunohistochemistry, and the quantitative analyses of the mean number and mean area of silver-stained nucleolar organizer regions (AgNORs) per one nucleus were performed for all these cases. All cancers and adenomas were positively stained by the DNA-instability test diffusely, indicating the malignant character of the latter from the view point of DNA-instability, in contrast to the negative stainability of all regenerative epithelium. The percent number of PCNA-positive cells and mean number and mean area of AgNORs tended to be larger in adenoma and cancer than in regenerative epithelium, although the differences were not usually statistically significant. Supporting the malignant character of adenoma, single cell necroses and abnormal mitoses were almost always present in the lesion. In conclusion, all adenoma lesions were regarded as malignant in nature, namely, in-situ carcinoma, existing at an early stage of progression of malignancy.
Otaki
T
T
Second Department of Internal Medicine, Fukui Medical School, Japan.
Kohli
Y
Y
Fujiki
N
N
Imamura
Y
Y
Fukuda
M
M
D015100:3,3'-Diaminobenzidine; D000230:Adenocarcinoma / Q000473:pathology; D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000502:physiology; D004273:DNA, Neoplasm / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D018450:Disease Progression; D006801:Humans; D006868:Hydrolysis; D007150:Immunohistochemistry; D008938:Mitosis; D016612:Paraffin Embedding; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012260:Ribonucleases / Q000737:chemistry; D016622:Silver Staining; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D013276:Stomach Ulcer / Q000473:pathology
D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D018809:Proliferating Cell Nuclear Antigen; D015100:3,3'-Diaminobenzidine; D012260:Ribonucleases