E-selectin expression in salivary endothelial cells and sera from patients with systemic sclerosis. Role of resident mast cell-derived tumor necrosis factor alpha.
OBJECTIVE
To assess endothelial cell activation in patients with systemic sclerosis (SSc).


METHODS
Concomitant study of salivary gland biopsy tissues and sera for expression of E-selectin and its potent activator tumor necrosis factor alpha (TNF alpha), using immunostaining and enzyme-linked immunosorbent essay.


RESULTS
E-selectin was overexpressed in SSc patients, but not in controls. TNF alpha was detected in mast cells.


CONCLUSIONS
Mast cell-derived TNF alpha may contribute to endothelial cell activation in SSc.
Hebbar
M
M
Unité INSERM 416, Institut Pasteur, Lille, France.
Lassalle
P
P
Janin
A
A
Vanhée
D
D
Bisiau
S
S
Hatron
P Y
PY
Tonnel
A B
AB
Gosselin
B
B
D000293:Adolescent; D000328:Adult; D000368:Aged; D001706:Biopsy; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000097:blood; D019040:E-Selectin; D004727:Endothelium / Q000737:chemistry; D005260:Female; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000737:chemistry; D008875:Middle Aged; D012470:Salivary Glands, Minor / Q000737:chemistry* / Q000473:pathology; D012595:Scleroderma, Systemic / Q000503:physiopathology*; D012859:Sjogren's Syndrome / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D014409:Tumor Necrosis Factor-alpha