title: Inhibition of degranulation of human polymorphonuclear leukocytes by complement factor D.
abstract: A degranulation inhibiting protein could be isolated from human plasma ultrafiltrate by a three-step purification method including ion-exchange chromatography, gelfiltration and affinity-chromatography. The protein was identified as complement factor D by means of sequence analysis. Its degranulation inhibiting activity was determined with regard to its effect on the FNLPNTL-induced lactoferrin secretion of human polymorphonuclear leukocytes. Complement factor D caused a dose-dependent decrease of the FNLPNTL-stimulated lactoferrin degranulation down to 34% of stimulated controls.
authors.affiliation: Department of Biochemistry, Faculty of Chemistry, University of Bielefeld, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Balke
authors.forename: U
authors.initials: U
authors.lastname: Holtkamp
authors.forename: W H
authors.initials: WH
authors.lastname: Hörl
authors.forename: H
authors.initials: H
authors.lastname: Tschesche
mesh_terms: D000595:Amino Acid Sequence; D015550:Cell Degranulation / Q000502:physiology*; D011416:Complement Factor D / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000502:physiology*; D014462:Ultrafiltration
chemical_list: C493488:CFD protein, human; D011416:Complement Factor D