Use of differentiated cholinergic and second messenger endpoints to evaluate cholinesterase inhibitors.
1. The effects of organophosphorus compounds on the cholinergic properties of a mouse septal neuronal cell line, SN56, were investigated. Treatment of the cells with 10 mumol/L paraoxon or 10 mumol/L diisopropylphosphorofluoridate (DFP) resulted, respectively, in rapid 27- and 11-fold increases of intracellular acetylcholine content. 2. Time-course studies showed that paraoxon maintained its efficacy whereas the effects of DFP decayed quickly, possibly due to rapid hydrolysis and inactivation of DFP in the growth medium.
Blusztajn
J K
JK
Boston University School of Medicine, MA 02118, USA.
Davis
R O
RO
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002800:Cholinesterase Inhibitors / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D007531:Isoflurophate / Q000633:toxicity; D051379:Mice; D009388:Neostigmine / Q000633:toxicity; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D009943:Organophosphorus Compounds / Q000633:toxicity*; D010261:Paraoxon / Q000633:toxicity; D015290:Second Messenger Systems / Q000187:drug effects; D012686:Septal Nuclei / Q000166:cytology / Q000187:drug effects*
D002800:Cholinesterase Inhibitors; D009943:Organophosphorus Compounds; D007531:Isoflurophate; D009388:Neostigmine; D000109:Acetylcholine; D010261:Paraoxon