Electron-microscopic and immunohistochemical studies of Langerhans cells and Thy-1-positive cells in mouse tongue epithelium subjected to local hyperthermia.
Local hyperthermia via skin has been used to treat cancer but may suppress local immune responses as a side-effect. To examine effects of heat on immunologically responsive cells in oral mucosa, mouse tongues were heated by an implant system at 43 degrees C for 20 min. The densities of Langerhans cells and Thy-1-positive cells rapidly increased within 3 h after the treatment, then returned to a normal level after 7 days. Electron microscopy confirmed that Langerhans cells in the tongue epithelium formed clusters with lymphocytic cells, suggesting an active response to the hyperthermia.
Mitsudo
K
K
Department of Oral Surgery, Nagoya University, School of Medicine, Japan.
Kobayashi
M
M
Tohnai
I
I
Ueda
M
M
Kotani
H
H
Hoshino
T
T
D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000276:immunology / Q000473:pathology; D002452:Cell Count; D004848:Epithelium / Q000276:immunology / Q000473:pathology; D005260:Female; D005455:Fluorescent Antibody Technique; D006979:Hyperthermia, Induced / Q000295:instrumentation; D007150:Immunohistochemistry; D007801:Langerhans Cells / Q000473:pathology*; D008214:Lymphocytes / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D009061:Mouth Mucosa / Q000276:immunology / Q000473:pathology; D013194:Staining and Labeling; D018800:Thy-1 Antigens / Q000032:analysis*; D014059:Tongue / Q000276:immunology / Q000473:pathology*
D018800:Thy-1 Antigens; D000251:Adenosine Triphosphatases