title: Conformational studies of a synthetic cyclic decapeptide fragment of rat transforming growth factor-alpha.
abstract: The solution conformation of a synthetic cyclic decapeptide [with sequence mimicking the third disulfide loop of rat transforming growth factor-alpha (rTGF-alpha)] in deuterated dimethyl sulfoxide was studied by 2D NMR. The determination of solution structures was based on NOE interproton distances, using a combination of distance geometry and simulated annealing protocols. The convergence of the selected structures was evident from the small atomic pairwise root-mean-square deviation values among them. Good agreement was noted between the experimental and simulated NOESY spectra, thereby reflecting the accuracy of the calculated solution structures. Analysis of the structures indicates that the residues Tyr5 and Arg9 exhibit similar side chain orientation as that in the corresponding disulfide loop of human transforming growth factor-alpha.
authors.affiliation: Department of Chemistry, National Tsing Hua University, Hsinchu, Taiwan, ROC.
authors.forename: G
authors.initials: G
authors.lastname: Jayaraman
authors.forename: R
authors.initials: R
authors.lastname: Bhaskaran
authors.forename: T K
authors.initials: TK
authors.lastname: Kumar
authors.forename: H M
authors.initials: HM
authors.lastname: Yu
authors.forename: S T
authors.initials: ST
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000737:chemistry; D002942:Circular Dichroism; D003196:Computer Graphics; D004220:Disulfides / Q000737:chemistry; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000138:chemical synthesis / Q000737:chemistry*; D011487:Protein Conformation; D051381:Rats; D016211:Transforming Growth Factor alpha / Q000737:chemistry*; D014443:Tyrosine / Q000737:chemistry
chemical_list: D004220:Disulfides; D010446:Peptide Fragments; D010456:Peptides, Cyclic; D016211:Transforming Growth Factor alpha; D014443:Tyrosine; D001120:Arginine