title: Down-regulation of CFTR expression in an HT29-derived cell line by stable antisense RNA production.
abstract: In order to examine the interactions of the cAMP activated CFTR chloride ion channel with other chloride ion channels present in epithelial cells we have generated a model based on antisense mRNA down regulation of CFTR expression. The HT29-derived epithelial cell line CJC4-1 has integrated a plasmid that is constitutively expressing CFTR exons 1-6 in the antisense orientation, from the MoMULV LTR. This cell line shows a reduction in cAMP-activated chloride efflux, as measured by iodide efflux assay, in comparison to the parental HT29 cell line and a sense transfected control.
authors.affiliation: Institute of Molecular Medicine, Oxford University, John Radcliffe Hospital, UK.
authors.forename: C J
authors.initials: CJ
authors.lastname: Wardle
authors.forename: A
authors.initials: A
authors.lastname: Harris
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D018118:Chloride Channels / Q000032:analysis*; D003550:Cystic Fibrosis / Q000235:genetics; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D015536:Down-Regulation; D004848:Epithelium / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D016372:RNA, Antisense / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection
chemical_list: C505032:CFTR protein, human; D018118:Chloride Channels; D008565:Membrane Proteins; D016372:RNA, Antisense; D012333:RNA, Messenger; D019005:Cystic Fibrosis Transmembrane Conductance Regulator