CD44 participates in tumor cell adhesion to endothelial cells in the experimental metastatic process in B16BL6 melanoma cells.
There are several pieces of evidence suggesting a relationship between CD44 expression and tumor metastasis, but the role of CD44 in the metastatic processes is unclear. We analysed the role of CD44 in the experimental metastatic processes of B16BL6 melanoma cells using anti-CD44 monoclonal antibody (clone IM7). B16BL6 melanoma cells expressed CD44 and these cells treated with anti-CD44 monoclonal antibody increased in the experimental metastatic ability, indicating that CD44 participates in metastatic processes in B16BL6 cells. Furthermore, the adhesiveness of B16BL6 cells to endothelial cells and the retention of these cells in the lung increased by treatment with anti-CD44 monoclonal antibody. These results suggested that the CD44-mediated adhesion of tumor cells to endothelial cells was involved in the experimental metastatic process of B 16BL6 cells.
Ota
T
T
Department of Pathology, Kanazawa Medical University, Ishikawa, Japan.
Matsui
T
T
Kohno
H
H
Maeda
M
M
Tanino
M
M
Odashima
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002352:Carrier Proteins / Q000502:physiology*; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000166:cytology*; D018960:Hyaluronan Receptors; D016169:Lymphocyte Function-Associated Antigen-1 / Q000502:physiology; D008297:Male; D008546:Melanoma, Experimental / Q000556:secondary*; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D011956:Receptors, Cell Surface / Q000502:physiology*; D016201:Receptors, Lymphocyte Homing / Q000502:physiology*; D016029:Receptors, Very Late Antigen / Q000502:physiology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D016169:Lymphocyte Function-Associated Antigen-1; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D016029:Receptors, Very Late Antigen