title: Effects of dietary fatty acid composition on basal and hormone-stimulated hepatic lipogenesis and on circulating lipids in the rat.
abstract: Thirty male rats were randomly assigned to one of three dietary groups in which the source of dietary fat was either a mixed oil, maize oil or fish oil. Effects of dietary fatty acid composition on in vitro rates of [U-14C]glucose incorporation into hepatic total lipids and into hepatic triacylglycerol were measured under basal, insulin (4 nM)-, gastric inhibitory polypeptide (GIP; 6 nM)- and insulin + GIP (4 nM + 6 nM)-stimulated conditions. Effects of the three diets on postprandial plasma triacylglycerol, cholesterol, insulin and GIP concentrations were also measured. The fish-oil diet decreased rates of basal glucose incorporation into hepatic total lipids (P < 0.05) and hepatic triacylglycerol, (P < 0.01) compared with the mixed-oil diet. The presence of insulin + GIP in the incubation medium stimulated glucose incorporation into hepatic total lipids in the maize-oil (P < 0.01) and fish-oil groups (P < 0.05), as well as into hepatic triacylglycerol in the maize-oil group (P < 0.005). In addition, the fish-oil diet decreased postprandial plasma triacylglycerol levels compared with both other dietary groups (P < 0.05 both cases), and the mixed-oil diet markedly increased postprandial plasma insulin levels compared with the other dietary groups (P < 0.001).
authors.affiliation: Nutritional Metabolism Research Group, School of Biological Sciences, University of Surrey, Guildford.
authors.forename: A
authors.initials: A
authors.lastname: Zampelas
authors.forename: L M
authors.initials: LM
authors.lastname: Morgan
authors.forename: N
authors.initials: N
authors.lastname: Furlonger
authors.forename: C M
authors.initials: CM
authors.lastname: Williams
mesh_terms: D000818:Animals; D001835:Body Weight; D003314:Corn Oil; D004435:Eating; D005227:Fatty Acids / Q000008:administration & dosage* / Q000737:chemistry; D005395:Fish Oils; D005749:Gastric Inhibitory Polypeptide / Q000097:blood; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000096:biosynthesis* / Q000097:blood; D008099:Liver / Q000033:anatomy & histology / Q000378:metabolism*; D008297:Male; D009929:Organ Size; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood
chemical_list: D005227:Fatty Acids; D005395:Fish Oils; D007328:Insulin; D008055:Lipids; D014280:Triglycerides; D005749:Gastric Inhibitory Polypeptide; D003314:Corn Oil