title: Effect of fibronectin on the production of leukotriene C4 by eosinophils.
abstract: The effect of fibronectin on leukotriene C4 (LTC4) production by eosinophils was examined. Eosinophils obtained from patients with bronchial asthma were cultured for 3 h with or without fibronectin and culture supernatants were assayed for LTC4 by specific enzyme immunoassay. LTC4 was produced in a time- and dose-dependent manner in the presence of fibronectin, and maximum production of LTC4 was observed 3 h after addition of 100 micrograms/ml of fibronectin. These results suggest that eosinophils may be activated by interaction with extracellular matrix proteins, including fibronectin, and play a role in the pathogenesis of bronchial asthma.
authors.affiliation: Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Suko
authors.forename: G
authors.initials: G
authors.lastname: Matsuzaki
authors.forename: H
authors.initials: H
authors.lastname: Sugiyama
authors.forename: H
authors.initials: H
authors.lastname: Okudaira
authors.forename: K
authors.initials: K
authors.lastname: Ito
mesh_terms: D001249:Asthma / Q000378:metabolism / Q000473:pathology; D004804:Eosinophils / Q000378:metabolism*; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D017997:Leukotriene C4 / Q000096:biosynthesis*
chemical_list: D005353:Fibronectins; D017997:Leukotriene C4