Generation of giant protoplasts of Escherichia coli and an inner-membrane anion selective conductance.
Established methods were modified and combined to generate unilamellar giant protoplasts in order to study the electric events on the cytoplasmic membrane of Escherichia coli with patch-clamp technique. The activities of many types of conductances were encountered, one of them is characterized here. This channel conductance is 109 pS in 150 mM KCl; it prefers anions, it is highly voltage dependent and is blocked by micromolar concentrations of anthracene-9-carboxylic acid.
Kubalski
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Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA.
D000838:Anions; D000873:Anthracenes / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology*; D018118:Chloride Channels / Q000037:antagonists & inhibitors; D004926:Escherichia coli / Q000502:physiology* / Q000648:ultrastructure; D017136:Ion Transport; D018408:Patch-Clamp Techniques; D011523:Protoplasts
D000838:Anions; D000873:Anthracenes; D018118:Chloride Channels; C010030:9-anthroic acid