Cloning and functional analysis of TipE, a novel membrane protein that enhances Drosophila para sodium channel function.
Voltage-dependent sodium channels are involved in the initiation and propagation of action potentials in many excitable cells. Here we report that tipE, a gene defined by a temperature-sensitive paralytic mutation in Drosophila, encodes a novel integral membrane protein that dramatically stimulates functional expression in Xenopus oocytes of the Drosophila sodium channel alpha subunit encoded by the paralytic (para) locus. Using a heat shock promoter to control tipE+ gene expression in transgenic flies, we demonstrate that tipE+ gene expression is required during pupal development to rescue adult paralysis. In addition, we demonstrate a role for the tipE gene product in adults.
Feng
G
G
Department of Biochemical Pharmacology, State University of New York at Buffalo 14260-1200, USA.
Deák
P
P
Chopra
M
M
Hall
L M
LM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018388:Codon, Terminator / Q000235:genetics; D018076:DNA, Complementary / Q000032:analysis / Q000302:isolation & purification; D004330:Drosophila; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D017344:Genes, Insect / Q000502:physiology; D006358:Hot Temperature; D008565:Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D009865:Oocytes / Q000502:physiology / Q000648:ultrastructure; D010243:Paralysis / Q000235:genetics; D011679:Pupa / Q000235:genetics / Q000648:ultrastructure; D015222:Sodium Channels / Q000235:genetics*; D014981:Xenopus
D018388:Codon, Terminator; D018076:DNA, Complementary; D029721:Drosophila Proteins; D008565:Membrane Proteins; D015222:Sodium Channels; C095833:TipE protein, Drosophila