title: Structure and epitope specificity of the O-specific polysaccharide of Proteus penneri strain 12 (ATCC 33519) containing the amide of D-galacturonic acid with L-threonine.
abstract: O-specific polysaccharide was isolated from Proteus penneri strain 12 (ATCC 33519) lipopolysaccharide (LPS) and studied using NMR spectroscopy, including selective spin-decoupling, one-dimensional NOE, two-dimensional homonuclear correlation spectroscopy, 13C,1H heteronuclear correlation spectroscopy and chemical methods (O-deacetylation, Smith degradation, partial acid hydrolysis followed by borohydride reduction and methylation). The amide of D-galacturonic acid with L-threonine [D-GalA(L-Thr)] was identified as a constituent of the polysaccharide and the following structure of the tetrasaccharide repeating unit was established: [formula: see text] where the degree of O-acetylation at either position varies over 20-40%. Serological study with LPS, its degradation products and related synthetic glycoconjugates (2-acrylamidoethyl glycosides of amides of alpha-D-GalA with L-amino acids copolymerised with acrylamide) showed that D-GalA(L-Thr) plays an important role in manifesting the serological specificity of the P. penneri 12 O-antigen. Serological cross reactions between LPSs of P. penneri 12 and Proteus mirabilis S1959, R14/S1959 (transient-like form), O23 and O28 are discussed.
authors.affiliation: Institute of Microbiology and Immunology, University of Lodz, Poland.
authors.forename: Z
authors.initials: Z
authors.lastname: Sidorczyk
authors.forename: A
authors.initials: A
authors.lastname: Swierzko
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: E V
authors.initials: EV
authors.lastname: Vinogradov
authors.forename: A Y
authors.initials: AY
authors.lastname: Chernyak
authors.forename: L O
authors.initials: LO
authors.lastname: Kononov
authors.forename: M
authors.initials: M
authors.lastname: Cedzynski
authors.forename: A
authors.initials: A
authors.lastname: Rozalski
authors.forename: W
authors.initials: W
authors.lastname: Kaca
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
mesh_terms: D000577:Amides / Q000737:chemistry; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000737:chemistry* / Q000276:immunology; D006603:Hexuronic Acids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology; D011511:Proteus / Q000737:chemistry*; D012699:Serology; D013912:Threonine / Q000737:chemistry*
chemical_list: D000577:Amides; D000939:Epitopes; D006603:Hexuronic Acids; D019081:O Antigens; D011135:Polysaccharides, Bacterial; D013912:Threonine; C007819:galacturonic acid