CD11/CD18 panel report for swine CD workshop.
Five monoclonal antibodies (mAbs), PNK-I (W #037), H20A (W #077), MUC76A (W #078), MUC93A (W #079) and MHM23 (W #136) of CD11/18 panels reacted with 80-96% of porcine PBMC and PMN. Epitope mapping studies by competitive binding of mAb by flow cytometric analysis based on PNK-I as CD18 epitope defining antibody resulted H20A and MHM23 mAbs bind to the same shared epitope as PNK-I, but MUC76A and MUC93A mAbs were distinct from PNK-I. Thus, PNK-I, H20A and MHM23 were designated as CD18a mAbs.
Kim
Y B
YB
Department of Microbiology and Immunology, University of Health Sciences, Chicago Medical School, IL 60064.
Zhang
J
J
Davis
W C
WC
Lunney
J K
JK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D001667:Binding, Competitive / Q000276:immunology; D018845:CD11 Antigens / Q000276:immunology*; D018821:CD18 Antigens / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry / Q000662:veterinary; D007963:Leukocytes, Mononuclear / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D013552:Swine / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018845:CD11 Antigens; D018821:CD18 Antigens; D000939:Epitopes