Interleukin 1 beta up-regulates the expression of sulfoglucuronosyl paragloboside, a ligand for L-selectin, in brain microvascular endothelial cells.
Treatment of cultured bovine brain microvascular endothelial cells (BMECs) with interleukin 1 beta (IL-1 beta), an inflammatory cytokine, was shown to induce the accumulation of sulfoglucuronosyl paragloboside (SGPG), a glycolipid bearing the HNK-1 epitope. This resulted in the attachment of a greater number of human lymphocytes to the treated than to the untreated BMEC monolayers. Attachment of human lymphocytes to the IL-1 beta-activated BMEC cells could be blocked either by incubation of the human lymphocytes with an anti-L-selectin antibody or by application of an anti-SGPG antibody to the BMECs. These results suggest that SGPG may act as an important ligand for L-selectin for the regulation of the attachment of activated lymphocytes and their subsequent invasion into the nervous system parenchyma in inflammatory disorders of the central and peripheral nervous systems.
Kanda
T
T
Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0614, USA.
Yamawaki
M
M
Ariga
T
T
Yu
R K
RK
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002417:Cattle; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D005915:Globosides / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D019041:L-Selectin; D008024:Ligands; D008214:Lymphocytes / Q000276:immunology* / Q000502:physiology; D008833:Microcirculation; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology
D000906:Antibodies; D015815:Cell Adhesion Molecules; D005915:Globosides; D007375:Interleukin-1; D008024:Ligands; D016201:Receptors, Lymphocyte Homing; D011994:Recombinant Proteins; C051917:sulfate-3-glucuronyl paragloboside; D019041:L-Selectin