title: Cytokeratin expression in the developing vagina of the postnatal gerbil (Meriones unguiculatus).
abstract: During postnatal development the vaginal epithelium of the Mongolian gerbil is transformed from two to three layers into a stratified, first mucified subsequently keratinized squamous epithelium. Changes in the expression of cytokeratins were studied and the immunohistochemical results compared with the ultrastructural findings at the corresponding stage. The first 10 postnatal days (days pn) were characterized by a moderate, positive immunoreaction for pancytokeratin in all vaginal cell layers. A faint reaction was caused by mAB CK 18.01 against CK 1, 5, 6 and 8. The appearance of mucous granules in the luminal cells after 15 pn seemed to coincide with an increase in cytokeratins. The immunoresponse for pancytokeratin in these cells was very intense compared with the reaction in the basal cell layers. Mucocytes during development and at proestrus were the only cells which reacted faintly positive with mAB against CK 18 alone. The keratinizing epithelium, which differentiates after day 40 pn, reacted strongly positive for pancytokeratin in the keratinizing layers, desquamating, fully keratinized cells, however, showed a negative reaction. The data indicate that mucocytes are not transformed squamous keratinized cells, but represent a cell category with its individual differentiation potential. Vimentin was not expressed. Neither the epithelium of the sinus vagina nor of the Müllerian vagina displayed any response.
authors.affiliation: Department of Anatomy, University of Basel, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Kress
authors.forename: K
authors.initials: K
authors.lastname: Spanel-Borowski
authors.forename: A
authors.initials: A
authors.lastname: Ricken
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D004847:Epithelial Cells; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D004971:Estrus; D005260:Female; D005849:Gerbillinae; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis / Q000096:biosynthesis*; D016253:Microscopy, Immunoelectron; D011359:Proestrus; D014621:Vagina / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D014746:Vimentin / Q000032:analysis
chemical_list: D014746:Vimentin; D007633:Keratins