title: Structure of the capsular polysaccharide of Vibrio cholerae O139 synonym Bengal containing D-galactose 4,6-cyclophosphate.
abstract: The capsular polysaccharide (CPS) of Vibrio cholerae O139 synonym Bengal, which is thought to carry determinants of O-specificity, was isolated by phenol/water extraction followed by delipidation of the contaminating lipopolysaccharide at pH 4.2 and gel-permeation chromatography. The CPS contained D-galactose, 3,6-dideoxy-L-xylo-hexose (colitose, Col), 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2,6-dideoxy-D-glucose (N-acetyl-D-quinovosamine, D-QuiNAc), D-galacturonic acid (D-GalA), and phosphate. The CPS was studied by NMR spectroscopy, methylation analysis, and selective degradations, including partial acid hydrolysis at pH 3.1 and dephosphorylation with aqueous 48% hydrofluoric acid, which both resulted in complete cleavage of Col. It was concluded that the CPS is built up of hexasaccharide repeating units containing inter alia D-galactose 4,6-cyclophosphate and having the following structure [structure: see text] These data basically confirm the structure of the V. cholerae CPS proposed on the basis of an NMR study [L. M. Preston et al. (1995) J. Bacteriol. 177, 835-838] and specify exactly the absolute configurations of the constituent monosaccharides and the position of the cyclic phosphate.
authors.affiliation: Karolinska Institute, Clinical Research Centre, Huddinge University Hospital, Sweden.
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: L
authors.initials: L
authors.lastname: Paredes
authors.forename: P E
authors.initials: PE
authors.lastname: Jansson
authors.forename: A
authors.initials: A
authors.lastname: Weintraub
authors.forename: G
authors.initials: G
authors.lastname: Widmalm
authors.forename: M J
authors.initials: MJ
authors.lastname: Albert
mesh_terms: D002240:Carbohydrate Sequence; D005695:Galactosephosphates / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D014734:Vibrio cholerae / Q000737:chemistry*
chemical_list: D005695:Galactosephosphates; D011135:Polysaccharides, Bacterial; C095960:galactose 4,6-cyclophosphate