Alterations in leukocyte adhesion molecule expression after burn injury.
OBJECTIVE
To determine if thermal injury alters the expression of leukocyte adhesion molecules.


METHODS
This is a controlled experimental animal study.


METHODS
Partial thickness burns were created on the backs of New Zealand White rabbits. At 30 minutes, 2 hours, 4 hours, and 24 hours after burn, skin was harvested for immunohistochemistry. Monoclonal antibodies were used to study changes in intercellular adhesion molecule 1 (ICAM-1), E-selectin, and leukocyte CD11a. Staining was graded on a scale of 0 to 4.


RESULTS
ICAM-1 was significantly decreased at 24 hours after burn (p < 0.007, Wilcoxon signed rank test). CD11a was increased at 30 minutes (p < 0.02), 2 hours (p < 0.02), and 24 hours (p < 0.006). E-selectin was increased at 2 hours (p < 0.03).


CONCLUSIONS
Thermal injury alters the expression of leukocyte adhesion molecules.
Nwariaku
F E
FE
Department of Surgery, University of Texas Southwestern Medical Center at Dallas 75235-9031, USA.
Mileski
W J
WJ
Lightfoot
E
E
Sikes
P J
PJ
Lipsky
P E
PE
D000818:Animals; D000911:Antibodies, Monoclonal; D002056:Burns / Q000276:immunology* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000378:metabolism; D019040:E-Selectin; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007957:Leukocyte Adherence Inhibition Test; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D011817:Rabbits
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1