Effect of anticancer drugs on the release of TGF-beta in vitro.
Some conventional and experimental anticancer drugs were tested for their effect on Concanavalin A (Con A)-induced Transforming Growth Factor-beta (TGF-beta) release from BALB/c splenocytes, lipopolysaccharide (LPS)-induced TGF-beta release from Nude mouse splenocytes and BALB/c peritoneal exudate cells stimulated by LPS in vitro. When 5 x 10(6) BALB/c and Nude mouse splenocytes/ml stimulated with 1 microgram/ml Con A, 50ng/ml LPS respectively, and 5 x 10(6)/ml peritoneal exudate cells stimulated with 50ng/ml LPS were incubated with various non-cytotoxic concentrations of the vinca alkaloid Vincristine, there was an inhibition of the release of TGF-beta in culture supernatants of both BALB/c splenocytes and peritoneal exudate cells. But, in Nude mouse Vincristine did not alter the release of TGF-beta. The antitumour antibiotic Bleomycin, the immunoactive peptide FK565 and the immunosuppressive agent Cyclosporin A (CsA) were found to have no effect on the release of TGF-beta from all culture supernatants.
Kassem
S M
SM
Department of Pathology, Royal College of Surgeons of England, London.
Blaney
B A
BA
Turk
J L
JL
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001681:Biological Assay; D001761:Bleomycin / Q000494:pharmacology; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D016572:Cyclosporine / Q000494:pharmacology; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008907:Mink; D009842:Oligopeptides / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000166:cytology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*; D014750:Vincristine / Q000494:pharmacology
D000970:Antineoplastic Agents; D009842:Oligopeptides; D016212:Transforming Growth Factor beta; D003208:Concanavalin A; D001761:Bleomycin; D014750:Vincristine; D016572:Cyclosporine; C039537:heptanoyl-gamma-D-glutamyl-L-meso-diaminopimelyl-D-alanine