Effect of vitamin E on keratinocyte-modulation induced by lauroylsarcosine.
The effect of vitamin E on the modulation of keratinocytes was studied in rats. A 1% lauroylsarcosine (LS) ointment caused skin erythema with keratinocyte-damage. A 30% vitamin E ointment markedly alleviated this erythema and protected keratinocytes from cell damage. Vitamin E (100 micrograms/ml) was also effective on LS (7.5 micrograms/ml)-induced proliferative reduction of cultured keratinocytes. On the other hand, ointment containing superoxide dismutase (SOD) (99,000 U/g) decreased the LS-induced erythema, suggesting that superoxide anion (O2-) produced from keratinocytes play an important role in the skin irritation. Indeed, LS induced O2- production from cultured keratinocytes. The O2- was significantly reduced by vitamin E and SOD, although vitamin E had no effects on O2- production in a xanthine-xanthine oxidase system, unlike the effect observed with SOD. These results indicate that vitamin E is an inhibitor of keratinocyte-modulation.
Shimizu
T
T
Medical Research Laboratory, Sekisui Chemical Co., Ltd., Osaka, Japan.
Aioi
A
A
Horiguchi
T
T
Kuriyama
K
K
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003902:Detergents / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects; D004890:Erythema / Q000139:chemically induced / Q000188:drug therapy*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects*; D008297:Male; D009824:Ointments; D051381:Rats; D017208:Rats, Wistar; D012521:Sarcosine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D012882:Skin Tests; D013482:Superoxide Dismutase / Q000494:pharmacology / Q000627:therapeutic use; D013481:Superoxides / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology / Q000627:therapeutic use; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D019820:Xanthine; D014969:Xanthine Oxidase / Q000378:metabolism; D014970:Xanthines / Q000378:metabolism
D003902:Detergents; D009824:Ointments; D014970:Xanthines; D013481:Superoxides; D014810:Vitamin E; D019820:Xanthine; D014747:Vinblastine; C025231:sarkosyl; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D012521:Sarcosine