title: Cloning and mapping of the U2af1-rs2 gene with a high transmission distortion in interspecific backcross progeny.
abstract: We have cloned and analyzed the mouse U2af1-rs2 gene based on its sequence similarity to the imprinted gene U2af1-rs1 (SP2). Sequence analysis of this U2af1-rs2 cDNA revealed that it contained an open reading frame encoding a protein of 462 amino acid residues. The predicted amino acid sequence showed 72.7 and 35.8% identity to the U2af1-rs1 and U2 small nuclear ribonucleoprotein auxiliary factor, respectively. Interspecific backcross analysis showed this gene to map to the distal region of the X chromosome and also indicated that there was significant distortion of transmission ratio of the U2af1-rs2 allele in the backcrossed progeny from (C57BL/6J x Mus spretus)F1 females mated to Mus spretus males.
authors.affiliation: National Cardiovascular Center Research Institute, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamaoka
authors.forename: I
authors.initials: I
authors.lastname: Hatada
authors.forename: K
authors.initials: K
authors.lastname: Kitagawa
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: T
authors.initials: T
authors.lastname: Mukai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000737:chemistry; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D011110:Polymorphism, Genetic; D012261:Ribonucleoproteins
chemical_list: D018076:DNA, Complementary; D009687:Nuclear Proteins; D012261:Ribonucleoproteins; C096074:Zrsr2 protein, mouse