Differential induction of mRNA for ICAM-1 and selectins in hepatocytes, Kupffer cells and endothelial cells during endotoxemia.
Intercellular adhesion molecule-1 (ICAM-1) and selectins (E- and P-selectin) mRNAs were determined in individual liver cell types by Northern blot analysis before and after injection of endotoxin. A constitutive expression of ICAM-1 mRNA was found in endothelial cells and Kupffer cells but not in hepatocytes. All three cell types showed upregulation of ICAM-1 mRNA after endotoxin. No constitutive selectin expression could be detected in any liver cell, but endotoxin induced massive synthesis of E- and P-selectin mRNA in endothelial cells and Kupffer cells. The differential expression of cellular adhesion molecules in the liver is consistent with the involvement of selectins in neutrophil rolling in the vasculature and ICAM-1 in transendothelial migration and adherence to parenchymal cells.
Essani
N A
NA
Upjohn Company, Kalamazoo, MI 49001, USA.
McGuire
G M
GM
Manning
A M
AM
Jaeschke
H
H
D000704:Analysis of Variance; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D015342:DNA Probes; D019040:E-Selectin; D004727:Endothelium / Q000276:immunology / Q000378:metabolism; D015870:Gene Expression; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007728:Kupffer Cells / Q000276:immunology / Q000378:metabolism*; D008099:Liver / Q000276:immunology / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011916:Rats, Inbred F344
D015815:Cell Adhesion Molecules; D015342:DNA Probes; D019040:E-Selectin; D015345:Oligonucleotide Probes; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D018799:Intercellular Adhesion Molecule-1