Transfer of non-T-DNA portions of the Agrobacterium tumefaciens Ti plasmid pTiA6 from the left terminus of TL-DNA.
We introduced a plant selection marker, nptII, to the left of border A in the Agrobacterium Ti plasmid pTiA6. Infection of tobacco leaf discs with the modified Agrobacterium strain gave rise to kanamycin-resistant calli which grew in a hormone-dependent manner. Southern hybridization analysis of DNA isolated from four transformants indicated initiation of DNA transfer at or near border A and absence of T-DNA sequences. These results demonstrate that DNA transfer events starting at a left border on a native Ti plasmid and moving away from the T-DNA region occur and that they can be detected by designing a suitable selection strategy.
Ramanathan
V
V
Department of Plant Molecular Biology, School of Biotechnology, Madurai Kamaraj University, India.
Veluthambi
K
K
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D046508:Culture Techniques; D018014:Gene Transfer Techniques; D010947:Plants, Toxic; D010957:Plasmids; D012091:Repetitive Sequences, Nucleic Acid; D014026:Nicotiana