title: Urinary albumin fragments as a new clinical parameter for the early detection of diabetic nephropathy.
abstract: Urinary albumin fragments (uAF) from patients with NIDDM were analyzed as a possible factor in the early discovery of diabetic nephropathy before emergence of microalbuminuria. SDS-PAGE and immunoblot assay were employed for detection of uAF. Samples from 252 patients with NIDDM, 158 patients with non-diabetic diseases, and 48 healthy volunteers were examined; uAF were detected in 139 (55.2%), 94 (59.5%), only one (2.1%), respectively. In diabetic patients with normoalbuminuria, uAF were detected in 48 out of 159 cases (30.2%). Two years after the initial study, 3 of the 17 diabetic patients (17.6%) with normoalbuminuria and uAF showed micro- or macroalbuminuria. It was concluded that detection of uAF might be useful for the early detection of diabetic nephropathy in NIDDM.
authors.affiliation: Department of Internal Medicine, School of Medicine, Tokai University, Isehara.
authors.forename: M
authors.initials: M
authors.lastname: Yagame
authors.forename: D
authors.initials: D
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Jinde
authors.forename: N
authors.initials: N
authors.lastname: Yano
authors.forename: R
authors.initials: R
authors.lastname: Naka
authors.forename: Y
authors.initials: Y
authors.lastname: Abe
authors.forename: Y
authors.initials: Y
authors.lastname: Nomoto
authors.forename: H
authors.initials: H
authors.lastname: Sakai
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Ohashi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000419:Albuminuria / Q000209:etiology / Q000652:urine*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000652:urine*; D003928:Diabetic Nephropathies / Q000150:complications / Q000175:diagnosis* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D006801:Humans; D008875:Middle Aged; D010446:Peptide Fragments / Q000652:urine*; D015203:Reproducibility of Results
chemical_list: D010446:Peptide Fragments