Vaccination of sheep against haemonchosis with H11, a gut membrane-derived protective antigen from the adult parasite: prevention of the periparturient rise and colostral transfer of protective immunity.
Pregnant ewes were immunised with a fraction highly enriched in the membrane glycoprotein antigen H11, isolated from the intestinal brush border of adult Haemonchus contortus. Immunity induced by immunisation was able to abolish almost completely (98-99%) the worm egg output from pregnant ewes challenged with ca. 10,000 infective larvae of H. contortus during the last trimester. Furthermore, lambs born and reared on vaccinated ewes had substantial antibody levels to H11 derived from maternal transfer. This antibody conferred moderate protection against a bolus challenge of ca. 3000 infective larvae of H. contortus in 5-week-old lambs.
Andrews
S J
SJ
Mallinckrodt Veterinary Ltd., Harefield, Uxbridge, U.K.
Hole
N J
NJ
Munn
E A
EA
Rolph
T P
TP
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000008:administration & dosage* / Q000302:isolation & purification; D003126:Colostrum / Q000276:immunology; D005243:Feces / Q000469:parasitology; D005260:Female; D006188:Haemonchiasis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D006189:Haemonchus / Q000276:immunology*; D007112:Immunity, Maternally-Acquired; D007743:Labor, Obstetric; D008562:Membrane Glycoproteins / Q000008:administration & dosage / Q000276:immunology / Q000302:isolation & purification; D010270:Parasite Egg Count; D011247:Pregnancy; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000469:parasitology / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D008562:Membrane Glycoproteins