[Kinetic analysis of interaction of human immunodeficiency virus reverse transcriptase with alkaloids].
The interactions of HIV-I reverse transcriptase with some alkaloids were studied. Among nine compounds tested three--berberine, palmatine and sanguiritrine--inhibited RT. The dependence of the inhibition on the type of template-primer was also demonstrated. The kinetic analysis as well as circular dichroism experiments suggest the complex mechanism of RT inhibition by alkaloids. This mechanism includes both enzyme-alkaloid and alkaloid-template interactions; the latter effect also results in RT inhibition.
Gudima
S O
SO
Memelova
L V
LV
Borodulin
V B
VB
Pokholok
D K
DK
Mednikov
B M
BM
Tolkachev
O N
ON
Kochetkov
S N
SN
D000470:Alkaloids / Q000494:pharmacology*; D001599:Berberine / Q000494:pharmacology; D001600:Berberine Alkaloids / Q000494:pharmacology; D002942:Circular Dichroism; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology*; D007700:Kinetics; D018894:Reverse Transcriptase Inhibitors
D000470:Alkaloids; D001600:Berberine Alkaloids; D018894:Reverse Transcriptase Inhibitors; D001599:Berberine; C070444:sanguiritrine; D054303:HIV Reverse Transcriptase; C005413:palmatine