The effect of peroxides on the vascular endothelium of isolated pig aorta in vitro.
The effect of peroxide on endothelial cells (perfused pig aorta) was examined using an in vitro perfusion model. Hydrogen peroxide was added to the perfusion medium (pig serum together with a buffer solution) which was expected to lead to an increased oxidation of lipids and lipoproteins. Oxidation processes of this type play a decisive role in the pathogenesis and progression of arteriosclerosis. The aim of the present investigation was to demonstrate by introducing hydrogen peroxide (H2O2) in varying concentrations (0.5-1.5%), the destructive impact of peroxides on the endothelium, while these cells are believed to play a key role in the pathogenesis of arteriosclerosis. The extent of endothelial cell impairment was assessed by means of silver staining visualisation of endothelial cell borders as well as light- and scanning-electronmicroscopic investigation. It was discovered that the endothelial cells show increasing impairment after 10 h of perfusion due to the effect of peroxide (hydrogen peroxide).
Krautschick
I
I
Institute of Pathology, University Leipzig, Germany.
Krugmann
J
J
Neuenfeld
M
M
D000818:Animals; D001012:Aorta, Abdominal / Q000166:cytology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D006861:Hydrogen Peroxide / Q000633:toxicity*; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D010477:Perfusion / Q000379:methods; D016622:Silver Staining / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D013552:Swine
D006861:Hydrogen Peroxide