Synergistic action of interleukin-10 (IL-10) with IL-3, IL-4 and stem cell factor on colony formation from murine mast cells in culture.
We examined the effects of interleukin-10 (IL-10) on colony formation from two different types of murine mast cells, bone marrow-derived mast cells (BMMC) and serosal mast cells (SMC), using a methylcellulose culture method. IL-10 alone did not induce colony formation from either phenotype. However, BMMC and SMC produced colonies in the presence of IL-10 in combinations with IL-3, IL-4 or stem cell factor (SCF), but they responded in different manners. IL-10 enhanced the IL-3-dependent colony formation from BMMC and induced the colony formation from BMMC synergistically with IL-4 or SCF dose-dependently, although IL-4 and SCF were not active alone. The most significant synergism was observed between IL-10 and IL-4. The addition of IL-10 to the cultures of BMMC in the presence of two or three factors enhanced the colony formation induced by IL-3 plus IL-4, and inhibited the colony formation induced by IL-3 plus SCF or IL-3, IL-4 plus SCF. In the colony formation from SMC, IL-10 synergized with IL-3 but not with IL-4 or SCF. IL-10 in combination with two or three factors enhanced the colony formation from SMC induced by IL-3 plus IL-4, but did not affect the colony formation induced by other combinations among IL-3, IL-4 and SCF. These findings indicate that IL-10 plays an important role in the proliferation of murine mast cells.
Tsuji
K
K
Department of Clinical Oncology, University of Tokyo, Japan.
Koike
K
K
Komiyama
A
A
Miyajima
A
A
Arai
K
K
Nakahata
T
T
D000818:Animals; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004357:Drug Synergism; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D016753:Interleukin-10 / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology*; D008297:Male; D008407:Mast Cells / Q000166:cytology*; D051379:Mice; D008815:Mice, Inbred Strains; D019089:Stem Cell Factor
D016298:Hematopoietic Cell Growth Factors; D007377:Interleukin-3; D019089:Stem Cell Factor; D016753:Interleukin-10; D015847:Interleukin-4