title: Is endothelin-1 release at reperfusion of the ischaemic human heart due to cold-induced displacement of endothelin from binding sites?
abstract: Following reperfusion of ischaemic human hearts subjected to cold (4 degrees C) cardioplegia during coronary bypass surgery, there was an increase in cardiac outflow of endothelin-1 but not the pro-peptide big endothelin-1. Furthermore, specific endothelin-1 binding in human lung membrane preparations was displaced by incubation in buffer medium at 4 degrees C. The present results thus indicate that cold-induced displacement of endothelin-1 binding, rather than increased synthesis, may explain the cardiac release of endothelium-1 following ischaemia during heart surgery in which cold cardioplegia has been used.
authors.affiliation: Department of Thoracic Surgery, Karolinska Hospital, Stockholm, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Franco-Cereceda
authors.forename: G
authors.initials: G
authors.lastname: Barr
authors.forename: A
authors.initials: A
authors.lastname: Owall
authors.forename: J
authors.initials: J
authors.lastname: Liska
authors.forename: J M
authors.initials: JM
authors.lastname: Lundberg
mesh_terms: D000368:Aged; D003080:Cold Temperature; D001026:Coronary Artery Bypass; D019332:Endothelin-1; D016232:Endothelins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017202:Myocardial Ischemia / Q000378:metabolism*; D015425:Myocardial Reperfusion; D011498:Protein Precursors / Q000378:metabolism; D017466:Receptors, Endothelin / Q000378:metabolism*
chemical_list: D019332:Endothelin-1; D016232:Endothelins; D011498:Protein Precursors; D017466:Receptors, Endothelin