title: Effect of the PufQ protein on early steps in the pathway of bacteriochlorophyll biosynthesis in Rhodobacter capsulatus.
abstract: The addition in trans of the pufQ gene to a strain of Rhodobacter capsulatus from which the entire puf operon had been deleted, increased its ability to synthesize coproporphyrinogen from both delta-aminolevulinic acid and porphobilinogen. Studies at the enzyme level indicated that the conversion of porphobilinogen to uroporphyrinogen III had about a 2-fold higher level of activity in the anaerobically-grown pufQ-containing strain. This increase in activity over the puf-deletion strain appeared to occur during transitions from aerobic to semiaerobic growth conditions. These results indicated that the PufQ protein may exert a stimulatory effect quite early in the pathway of bacteriochlorophyll biosynthesis.
authors.affiliation: Department of Chemistry, Simon Fraser University, Burnaby, B.C., Canada.
authors.forename: S
authors.initials: S
authors.lastname: Fidai
authors.forename: J A
authors.initials: JA
authors.lastname: Dahl
authors.forename: W R
authors.initials: WR
authors.lastname: Richards
mesh_terms: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls / Q000096:biosynthesis*; D015964:Gene Expression Regulation, Bacterial; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D016354:Rhodobacter capsulatus / Q000254:growth & development / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; C087270:PufQ protein, Rhodobacter capsulatus