Gene targeting for inflammatory cell adhesion molecules.
Using gene targeting in mouse embryonic stem cells, it is possible to introduce diverse mutations into specific genes. Using these methods, various laboratories have reported mutations for a variety of inflammatory cell adhesion molecules including CD18, alpha 5 integrin, ICAM-1, P-selectin, and L-selectin; preliminary reports of other mutations are also available. Mutations in CD18 and ICAM-1 cause impaired inflammatory and immune responses, mutations in P-selectin and L-selectin cause decreased leukocyte rolling and emigration, and a mutation in alpha 5 integrin causes embryonic lethality. Gene targeting complements other approaches for analyzing the function of inflammatory cell adhesion molecules.
Bullard
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DC
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Sandberg
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ET
Scharffetter-Kochanek
K
K
Beaudet
A L
AL
D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000502:physiology; D018390:Gene Targeting; D007249:Inflammation / Q000235:genetics* / Q000276:immunology; D016023:Integrins / Q000235:genetics / Q000502:physiology; D019041:L-Selectin; D009154:Mutation; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000235:genetics / Q000502:physiology
D015815:Cell Adhesion Molecules; D016023:Integrins; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D019041:L-Selectin