title: Novel antiadhesion barrier does not prevent anastomotic healing in a rabbit model.
abstract: Intraperitoneal adhesions following surgical procedures cause considerable morbidity. Hyaluronic acid/carboxymethylcellulose (HA/CMC) films have been shown to be effective agents in decreasing adhesion formation. However, when there is an inadvertent leak of bowel contents into the peritoneum due to incomplete anastomosis, adhesion formation about a defect in order to prevent further leakage and to promote healing of the wound is important for the prevention of morbidity and mortality. The purpose of this study was to determine if an antiadhesion film (HA/CMC) impairs these potentially beneficial adhesions to bowel anastomoses, thus predisposing them to enteric leaks with subsequent peritonitis. Sixty-four rabbits were divided in two groups, each undergoing a complete or partial (90% anastomosis to simulate anastomotic leak) large bowel anastomosis. Half of each of the above groups were treated by wrapping a HA/CMC film over the anastomosis and the other half were untreated controls. These two subgroups were then further divided equally and sacrificed at either 7 or 14 days for evaluation of anastomosis integrity and strength. The average anastomotic bursting pressures did not change significantly between those groups treated with HA/CMC when compared to untreated controls at 7 or 14 days or in the complete or partial anastomosis group (Student's t test). Adhesion formation to the anastomosis was not impaired in either group independent of HA/CMC film application. This study suggests that while HA/CMC film has been shown to decrease adhesions in other models, healing of a rabbit colonic anastomosis even in the presence of an anastomotic defect takes place, further suggesting that the stimulus for adhesion formation can overcome the antiadhesion properties of HA/CMC.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, New England Medical Center, Boston, Massachusetts, USA.
authors.forename: M
authors.initials: M
authors.lastname: Medina
authors.forename: H N
authors.initials: HN
authors.lastname: Paddock
authors.forename: R J
authors.initials: RJ
authors.lastname: Connolly
authors.forename: S D
authors.initials: SD
authors.lastname: Schwaitzberg
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D001672:Biocompatible Materials; D001696:Biomechanical Phenomena; D002266:Carboxymethylcellulose Sodium; D003106:Colon / Q000503:physiopathology / Q000601:surgery*; D004195:Disease Models, Animal; D006820:Hyaluronic Acid; D008422:Materials Testing; D011817:Rabbits; D012449:Safety; D013997:Time Factors; D000267:Tissue Adhesions / Q000517:prevention & control*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D006820:Hyaluronic Acid; D002266:Carboxymethylcellulose Sodium