Analysis of immunoglobulin gene rearrangements in single B cells.
The polymerase chain reaction allows the characterization of RNA and DNA sequences in samples as small as a single cell. The recent development of amplification systems designed to isolate rearranged immunoglobulin genes from single B lineage cells has provided a powerful tool to investigate various aspects of B-cell development.
Ehlich
A
A
Institute for Genetics, University of Cologne, Germany.
Küppers
R
R
D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry*; D015322:Gene Rearrangement, B-Lymphocyte; D005803:Genes, Immunoglobulin; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*
D007135:Immunoglobulin Variable Region