Trophic effects of substance P and beta-amyloid peptide on dibutyryl cyclic AMP-differentiated human leukemic (HL-60) cells.
The neuropeptide substance P (SP) is a mediator of neurogenic inflammation. Also, beta-amyloid protein (beta AP) can directly activate the cell types involved in inflammatory processes. The relationship between SP and beta AP on their biological actions has attracted much interest. SP is trophic in neuronal cells and protected them against the death induced by beta AP. In this study, we examined the effects of SP and beta-amyloid peptide on cell viability in neutrophil-like HL-60 cells, by means of the WST-1 tetrazolium and lactate dehydrogenase (LDH) release assays. The results showed that SP promoted the cell survival on neutrophil-like HL-60 cells in serum-free conditions. Also, beta-amyloid peptide showed trophic effects rather than toxic in these cells in WST-1 assay, though it is reportedly toxic in neuronal cells.
Takenouchi
T
T
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Munekata
E
E
D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D016895:Culture Media, Serum-Free; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007938:Leukemia; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000494:pharmacology*; D013373:Substance P / Q000494:pharmacology*; D013481:Superoxides / Q000378:metabolism; D014407:Tumor Cells, Cultured
D016229:Amyloid beta-Peptides; D016895:Culture Media, Serum-Free; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); D013481:Superoxides; D013373:Substance P; D003994:Bucladesine; D007770:L-Lactate Dehydrogenase; D002118:Calcium