title: Resistance against multiple plant viruses in plants mediated by a double stranded-RNA specific ribonuclease.
abstract: Many plant viruses have single-stranded RNAs as their genomes. During the course of replication, genomic RNAs and their complementary template RNAs are likely to form double-stranded (ds) RNA at least transiently. To attack replication intermediates of many plant RNA viruses specifically, we introduced a yeast-derived ds-RNA specific RNase gene called pac 1 into plants. The transformed plants showed a decrease in lesion numbers when they were challenged with tomato mosaic virus, and a delay in the appearance of symptoms when inoculated with cucumber mosaic virus or potato virus Y.
authors.affiliation: Department of Biosciences, School of Science and Engineering, Teikyo University, Utsunomiya, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Ogawa
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: I
authors.initials: I
authors.lastname: Ishida
authors.forename: Y
authors.initials: Y
authors.lastname: Takeuchi
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
mesh_terms: D009029:Mosaic Viruses / Q000502:physiology*; D010944:Plants / Q000821:virology*; D030821:Plants, Genetically Modified; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012260:Ribonucleases / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D012330:RNA, Double-Stranded; D012260:Ribonucleases