Primary sequence, copy number, and distribution of mariner transposons in the honey bee.
A single honey bee mariner transposon (TnM1a) was sequenced, revealing a transpositionally non-autonomous element of 937 bp delimited by 30 bp perfect inverted terminal repeats. The element is flanked by the TA duplication typical of mariner elements in general. There are approximately 435 copies of TnM1a homologous elements per haploid genome. These elements appear, by Southern blot analysis, to be dispersed throughout the genome. Thirteen individual genomic clones with an average size of 15 kb, were found to contain only a single element each, which also suggests that the elements are not tightly clustered. Finally, mariner elements are neither inactivated by methylation nor sequestered into a methylated fraction of the genome.
Ebert
P R
PR
Department of Entomology, University of California, Davis 95616, USA.
Hileman
J P
JP
Nguyen
H T
HT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000235:genetics*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004247:DNA; D004251:DNA Transposable Elements / Q000235:genetics*; D005787:Gene Frequency; D015723:Gene Library; D017344:Genes, Insect; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D004251:DNA Transposable Elements; D004247:DNA