title: [Flow cytometry identification of CD34-positive cells].
abstract: In the present study three clinical applications of flow-cytometric quantification of CD34-positive cells in the field of blood and marrow transplantation are evaluated. By daily determination of CD34-positive cells in the peripheral blood after mobilization of stem cells with chemotherapy or growth-factors it is possible to set the optimal time for collection of the transplant material. Thereafter the yield of CD34-positive cells within the harvested material is determined. Finally, the efficacy of positive CD34 selection using a biotin-avidin column is evaluated. Initial experience is presented here.
authors.affiliation: Departement Zentrallaboratorium, Kantonsspital Basel.
authors.forename: A
authors.initials: A
authors.lastname: Tichelli
authors.forename: A
authors.initials: A
authors.lastname: Kralj
authors.forename: H
authors.initials: H
authors.lastname: Huxol
authors.forename: A
authors.initials: A
authors.lastname: Schönenberger
authors.forename: M
authors.initials: M
authors.lastname: Rabaglio
authors.forename: J
authors.initials: J
authors.lastname: Borel
authors.forename: R
authors.initials: R
authors.lastname: Müller
authors.forename: M
authors.initials: M
authors.lastname: Bargetzi
authors.forename: A
authors.initials: A
authors.lastname: Gratwohl
authors.forename: E
authors.initials: E
authors.lastname: Signer
mesh_terms: D015703:Antigens, CD / Q000302:isolation & purification*; D018952:Antigens, CD34; D001773:Blood Cells / Q000276:immunology / Q000637:transplantation; D016026:Bone Marrow Transplantation; D005434:Flow Cytometry; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34