title: Separation of antigenic glycoprotein fractions from cell-free homogenate of Pseudomonas pseudomallei and characterization as tyrosine phosphatase.
abstract: Cell-free extracts were prepared from Pseudomonas pseudomallei cells by freezing-thawing, sonication, and differential ultracentrifugation. The extracts were subjected to column chromatography with DEAE-sepharose to obtain glycoprotein fractions. The fractions showed acid phosphatase activity to p-nitrophenyl phosphate, tyrosine phosphate, serine phosphate, but not to threonine phosphate. They were highly antigenic when tested by immunofluorescence assay with the sera of melioidosis patients.
authors.affiliation: National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
authors.forename: E
authors.initials: E
authors.lastname: Kondo
authors.forename: P
authors.initials: P
authors.lastname: Wangroongsaub
authors.forename: P
authors.initials: P
authors.lastname: Naigowit
authors.forename: K
authors.initials: K
authors.lastname: Kanai
mesh_terms: D000818:Animals; D016957:Burkholderia pseudomallei / Q000201:enzymology*; D002474:Cell-Free System; D005591:Chemical Fractionation; D002847:Chromatography, Agarose; D006801:Humans; D017027:Protein Tyrosine Phosphatases / Q000302:isolation & purification*; D014461:Ultracentrifugation
chemical_list: D017027:Protein Tyrosine Phosphatases