Expression cloning of an interferon-inducible 17-kDa membrane protein implicated in the control of cell growth.
Interferon-inducible membrane proteins of approximately 17 kDa have been suggested to play a role in the antiproliferative activity of interferons based on (1) their pattern of induction in interferon-sensitive and -resistant cell lines and (2) the ability of a membrane fraction enriched in 17-kDa proteins to inhibit cell growth. To gain insight into the nature of the proteins that mediate the antiproliferative activity of interferons, a monoclonal antibody, 13A5, was generated that reacted specifically with a 17-kDa interferon-inducible cell surface protein. The expression pattern of this 17-kDa protein by human cell lines correlated with sensitivity to the antiproliferative activity of interferons. To obtain information regarding the structure of this protein, the 13A5 antibody was used to screen COS cells transfected with a human cDNA expression library. Sequence analysis of a full-length cDNA clone revealed identity with the 9-27 cDNA, previously isolated on the basis of its interferon inducibility by differential screening. In addition, the 17-kDa protein encoded by the 9-27 gene was shown to be identical to the Leu-13 antigen. Leu-13 was previously identified as a 16-kDa interferon-inducible protein in leukocytes and endothelial cells and is a component of a multimeric complex involved in the transduction of antiproliferative and homotypic adhesion signals. These results suggest a novel level of cellular regulation by interferons involving a membrane protein, encoded by the interferon-inducible 9-27 gene, which associates with other proteins at the cell surface, forming a complex relaying growth inhibitory and aggregation signals.
Deblandre
G A
GA
Département de Biologie Moléculaire, Université Libre de Bruxelles, Belgium.
Marinx
O P
OP
Evans
S S
SS
Majjaj
S
S
Leo
O
O
Caput
D
D
Huez
G A
GA
Wathelet
M G
MG
D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D006131:Growth Inhibitors / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011994:Recombinant Proteins; D014162:Transfection
D000911:Antibodies, Monoclonal; D017931:DNA Primers; D018076:DNA, Complementary; D006131:Growth Inhibitors; D007370:Interferon Type I; D008565:Membrane Proteins; D011994:Recombinant Proteins; D007371:Interferon-gamma