Rev-dependency of expression of human immunodeficiency virus type 1 gag and env genes.
Structural gene expression of human immunodeficiency virus type 1 (HIV-1) requires a viral regulatory protein, Rev transactivator. We investigated Rev-dependency of HIV-1 gene expression by various reporter systems. Expression of unspliced and single-spliced viral mRNAs was demonstrated to be differentially dependent on the Rev function. This difference of Rev-dependency was found not to be determined by cis-elements in gag, pol, and env coding sequences reported so far, and was lost when the reporter constructs containing minimum elements for Rev-responsiveness such as splice signals and rev responsive element were used for experiments. These findings indicated that the fundamental structure of HIV-1 mRNA was critical for the differential regulation of gene expression by Rev transactivator.
Sakai
H
H
Institute for Virus Research, Kyoto University, Japan.
Furuta
R A
RA
Tokunaga
K
K
Kawamura
M
M
Hatanaka
M
M
Adachi
A
A
D000818:Animals; D002415:Cats; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis / Q000096:biosynthesis; D002522:Chlorocebus aethiops; D003110:Colonic Neoplasms; D006224:Cricetinae; D015870:Gene Expression; D015967:Gene Expression Regulation, Viral; D015686:Gene Products, env / Q000096:biosynthesis*; D015683:Gene Products, gag / Q000096:biosynthesis*; D015694:Gene Products, rev / Q000378:metabolism*; D015752:Genes, env; D015750:Genes, gag; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007668:Kidney; D051379:Mice; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012194:RNA-Directed DNA Polymerase / Q000032:analysis / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D015533:Transcriptional Activation; D014162:Transfection; D054321:rev Gene Products, Human Immunodeficiency Virus
D015686:Gene Products, env; D015683:Gene Products, gag; D015694:Gene Products, rev; D012333:RNA, Messenger; D011994:Recombinant Proteins; D054321:rev Gene Products, Human Immunodeficiency Virus; D015500:Chloramphenicol O-Acetyltransferase; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase