Gene expression of keratinocyte and hepatocyte growth factors during the healing of rat gastric mucosal lesions.
OBJECTIVE
The factors that stimulate the growth of gastric mucosal cells during gastric mucosal healing are not completely understood. This study was designed to investigate the gene expression of keratinocyte growth factor (KGF) and hepatocyte growth factor (HGF) in the healing of gastric mucosal lesions.


METHODS
Northern blot analysis and reverse-transcription polymerase chain reaction were used to show HGF and KGF messenger RNA.


RESULTS
Transcripts of HGF and KGF were not shown in rat gastric mucosal epithelium but were found in submucosal and muscular layers under normal conditions. When acute gastric mucosal lesions were induced by indomethacin treatment, expression of HGF messenger RNA was augmented in submucosal, muscular, or serosal layers, whereas the transcript of KGF was not influenced. When rat gastric mucosal epithelial cell line RGM1 and rat gastric mucosal primary culture cells were incubated with HGF or KGF, their proliferation was enhanced.


CONCLUSIONS
The results showed increased gene expression of HGF together with constant production of KGF during gastric mucosal healing.
Kinoshita
Y
Y
Department of Medicine, Kobe University School of Medicine, Japan.
Nakata
H
H
Hassan
S
S
Asahara
M
M
Kawanami
C
C
Matsushima
Y
Y
Naribayashi-Inomoto
Y
Y
Ping
C Y
CY
Min
D
D
Nakamura
A
A
D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D051526:Fibroblast Growth Factor 10; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D005753:Gastric Mucosa / Q000737:chemistry / Q000502:physiology*; D015870:Gene Expression; D006133:Growth Substances / Q000235:genetics*; D017228:Hepatocyte Growth Factor / Q000235:genetics*; D017403:In Situ Hybridization; D007213:Indomethacin / Q000494:pharmacology; D015603:Keratinocytes / Q000502:physiology*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D051497:Receptor, Fibroblast Growth Factor, Type 2; D017468:Receptors, Fibroblast Growth Factor; D017978:Receptors, Growth Factor / Q000032:analysis; D013276:Stomach Ulcer / Q000378:metabolism
D000085:Acetates; C496455:Fgf7 protein, rat; D051526:Fibroblast Growth Factor 10; D006133:Growth Substances; D012333:RNA, Messenger; D017468:Receptors, Fibroblast Growth Factor; D017978:Receptors, Growth Factor; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D017228:Hepatocyte Growth Factor; D004247:DNA; D051497:Receptor, Fibroblast Growth Factor, Type 2; C065104:keratinocyte growth factor receptor; D019342:Acetic Acid; D007213:Indomethacin