Characterization of add(1)(p36) in non-Hodgkin lymphomas by fluorescence in situ hybridization.
Chromosome rearrangements involving chromosome I, band p36, are among the most common aberrations in non-Hodgkin lymphomas (NHL). We have studied nine cases of NHL with add(1)(p36) using fluorescence in situ hybridization (FISH) from a series of 205 cases. Five were follicular low-grade NHL and four were follicular or diffuse high-grade NHL. Three of the five cases with follicular low-grade NHL did not contain the 14;18 translocation. The extra material on the add(1)(p36) in these three cases was derived from chromosome segment 2q31-qter; in one it was observed as a sole clonal rearrangement. In the two remaining cases, with t(14;18), the add(1)(p36) consisted of material from chromosome arms 3q and 17q, respectively. In the four cases of high-grade NHL, the material added on to Ip36 was derived from chromosomes 6, 9, 17, and 19, respectively. Using a Ip36-specific probe, DIS94, we showed a deletion on the add(1) in one of the cases with low-grade NHL, whereas no loss was observed in one of the cases with high-grade NHL. Our study indicates that cytogenetically similar add(1)(p36) are found in both high- and low-grade NHL, and the breakpoint on Ip36 as well as the origin of translocated material may vary.
Bajalica
S
S
Department of Molecular Medicine, Karolinska Hospital, Stockholm, Sweden.
Brøndum-Nielsen
K
K
Sørensen
A G
AG
Pedersen
N T
NT
Kristoffersson
U
U
Akerman
M
M
Anderson
M
M
Pisa
P
P
Nordenskjöld
M
M
D015139:Blotting, Southern; D002878:Chromosomes, Human, Pair 1; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008224:Lymphoma, Follicular / Q000235:genetics; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014178:Translocation, Genetic