A potent, cost-effective RNase inhibitor.
The performance characteristics of a potent new RNase inhibitor have been evaluated, as have applications for its use in molecular biology. PRIME Inhibitor is a protein, not related to the commonly available human placental RNase inhibitors (HPRI). It has a high specific activity, enhanced temperature stability, broad reaction pH range and significantly greater cost-effectiveness than commercial HPRI. PRIME Inhibitor is suitable for use in in vitro transcription, in vitro translation, first- and second-strand cDNA synthesis, preparation of RNA and mRNA, and reverse transcription-polymerase chain reaction.
Murphy
N R
NR
Leinbach
S S
SS
Hellwig
R J
RJ
D000818:Animals; D016466:CHO Cells / Q000201:enzymology; D002478:Cells, Cultured / Q000201:enzymology; D003365:Costs and Cost Analysis; D006224:Cricetinae; D018076:DNA, Complementary; D016133:Polymerase Chain Reaction / Q000379:methods; D014176:Protein Biosynthesis; D012313:RNA / Q000302:isolation & purification*; D012260:Ribonucleases / Q000037:antagonists & inhibitors*; D012680:Sensitivity and Specificity; D014158:Transcription, Genetic
D018076:DNA, Complementary; D012313:RNA; D012260:Ribonucleases