RNA detection using non-radioactive in situ hybridization.
Methods of non-radioactive in situ hybridization to RNA now enable the simultaneous detection of two RNAs in the same tissue. Sensitivity has been increased by several modifications, including the use of the polymerase chain reaction. Recently, in situ hybridization has been combined with lineage tracing to determine the origin of cells expressing a specific gene.
Wilkinson
D G
DG
Laboratory of Developmental Neurobiology, National Institute for Medical Research, Mill Hill, London, UK.
D000818:Animals; D015342:DNA Probes; D015870:Gene Expression; D017403:In Situ Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012313:RNA / Q000032:analysis* / Q000096:biosynthesis; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis; D012680:Sensitivity and Specificity
D015342:DNA Probes; D015347:RNA Probes; D012333:RNA, Messenger; D012313:RNA