A homologue to the Escherichia coli alkyl hydroperoxide reductase AhpC is induced by osmotic upshock in Staphylococcus aureus.
Four major proteins are induced in Staphylococcus aureus in response to hyperosmotic shock caused by the presence of two different osmolytes, sucrose and NaCl. The gene encoding one of these proteins was isolated using a novel PCR procedure. The derived protein sequence shows extensive similarity to a subunit of alkyl hydroperoxide reductase (AhpC) from both Escherichia coli and Salmonella typhimurium. Exposure of S. aureus to varying concentrations of H202 did not result in the detectable induction of AhpC.
Armstrong-Buisseret
L
L
Department of Applied Biochemistry and Food Science, University of Nottingham, Faculty of Agricultural and Food Sciences, Leicestershire, UK.
Cole
M B
MB
Stewart
G S
GS
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009994:Osmolar Concentration; D018384:Oxidative Stress; D010088:Oxidoreductases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D010544:Peroxidases; D054464:Peroxiredoxins; D016133:Polymerase Chain Reaction / Q000379:methods; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012965:Sodium Chloride; D013395:Sucrose; D010088:Oxidoreductases; D010544:Peroxidases; D054464:Peroxiredoxins; C515937:ahpC protein, E coli