title: In vivo electrochemical monitoring of serotonin in spinal dorsal horn with Nafion-coated multi-carbon fiber electrodes.
abstract: Biosensors sensitive for in vivo monitoring of serotonin (5-HT) in the CNS by differential normal pulse voltammetry were constructed by coating treated multicarbon fiber electrodes (mCFEs) with Nafion (N-mCFE). In vitro sensitivities of mCFE and N-mCFE were compared in solutions ranging from 5 nM to 20 microM of uric acid (UA), 5-hydroxyindoleacetic acid (5-HIAA), and 5-HT. The mCFEs were three to seven times less sensitive for 5-HIAA or UA than for 5-HT. Nafion treatment dramatically decreased sensitivity for 5-HIAA and UA of N-mCFEs (approximately 10(3) times), whereas it remained in the nanomolar range for 5-HT. In vivo, in the dorsal horn of the lumbar spinal cord of anesthetized rats, the monoamine oxidase inhibitor clorgyline (10 mg/kg i.p.) produced a reduction (55 +/- 3% at 180 min) of peak 3 of oxidation current (characteristic of 5-hydroxyindoles) monitored with mCFEs, but with N-mCFEs (in this latter case the peak was termed 3N) peak 3N increased to 135 +/- 5% at 180 min. The 5-HT release-inducer p-chloroamphetamine (PCA; 6 mg/kg i.p.) induced a slight (12 +/- 3% at 150 min) decrease in peak 3 measured with mCFEs, whereas with N-mCFEs PCA induced a rapid increase of peak 3N (137 +/- 6% at 90 min). The xanthine oxidase inhibitor allopurinol (10 mg/kg i.p.) produced a decrease (30 +/- 3% at 180 min) in peak 3 (mCFEs), but peak 3N (N-mCFEs) was not affected (106% at 180 min). After pretreatment with allopurinol, PCA also produced an increase (135 +/- 6% at 90 min) in peak 3N.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Unité de Recherches de Physiopharmacologie du Système Nerveux (INSERM U. 161), Paris, France.
authors.forename: J P
authors.initials: JP
authors.lastname: Rivot
authors.forename: R
authors.initials: R
authors.lastname: Cespuglio
authors.forename: S
authors.initials: S
authors.lastname: Puig
authors.forename: M
authors.initials: M
authors.lastname: Jouvet
authors.forename: J M
authors.initials: JM
authors.lastname: Besson
mesh_terms: D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D015374:Biosensing Techniques; D003010:Clorgyline / Q000494:pharmacology; D004563:Electrochemistry; D004566:Electrodes; D005465:Fluorocarbon Polymers; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D012701:Serotonin / Q000032:analysis*; D013116:Spinal Cord / Q000737:chemistry*; D014527:Uric Acid / Q000032:analysis; D014969:Xanthine Oxidase / Q000037:antagonists & inhibitors; D010133:p-Chloroamphetamine / Q000494:pharmacology
chemical_list: D005465:Fluorocarbon Polymers; D014527:Uric Acid; D012701:Serotonin; C040402:perfluorosulfonic acid; D006897:Hydroxyindoleacetic Acid; D000493:Allopurinol; D010133:p-Chloroamphetamine; D014969:Xanthine Oxidase; D003010:Clorgyline