Detection and identification of Campylobacter coli and Campylobacter jejuni by two-step polymerase chain reaction.
Flagellin gene was used as target sequence to detect and distinguish C. coli and C. jejuni by a "nested PCR" technique. The method shows a high level of sensitivity and specificity. Application of this rapid diagnostic tool could provide further information about epidemiological and pathogenetic implications of each of these two microorganisms.
Comi
G
G
Department of Food Science, University of Udine, Italy.
Ferroni
P
P
Cocolin
L
L
Cantoni
C
C
Manzano
M
M
D001483:Base Sequence; D017000:Campylobacter coli / Q000302:isolation & purification*; D016123:Campylobacter jejuni / Q000302:isolation & purification*; D005408:Flagellin / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
D005408:Flagellin