Immunocytochemical detection of the carbohydrate antigen, Sialyl Lewis(x), in normal human skin and during irritant contact dermatitis.
Sialys Lewis(x) (SLex) is a ligand for the E-selectin and the interaction of E-selectin on the endothelium and SLex on T cells may be important for T-cell migration into the skin. We investigated the expression of SLex on Langerhans cells (LC) in normal skin and on LC repopulating epidermis deprived of LC due to a preceding irritant contact dermatitis. SLex was visualized by fluorescence and light microscopic immunocytochemistry using the monoclonal antibody, CSLEX-1. The results showed that about 40% of LC in normal epidermis express SLex. In the repopulation phase, most of the epidermal cells were CD1a+/SLex. We suggest that SLex is present on epidermal LC that have recently immigrated from the dermis.
Mikulowska
A
A
Department of Medical Cell Research, University of Lund, Sweden.
Ohlsson
L
L
Boketoft
A
A
Andersson
A
A
Falck
B
B
D002465:Cell Movement; D017453:Dermatitis, Irritant / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007801:Langerhans Cells / Q000378:metabolism / Q000473:pathology; D008297:Male; D009844:Oligosaccharides / Q000378:metabolism*; D012016:Reference Values; D000080506:Sialyl Lewis X Antigen; D012867:Skin / Q000378:metabolism* / Q000473:pathology; D013194:Staining and Labeling
D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen