The use of filter paper monoclonal antibodies in a Dot-blot test for typing Neisseria meningitidis B.
A simple method for the collection, preservation, shipment, and testing of minute amounts of dried monoclonal antibodies for typing Neisseria meningitidis B is described. The monoclonal antibodies collected on filter paper were extracted in PBS and evaluated by Dot-blot employing whole cells of N. meningitidis B as antigen. The dried filter paper with monoclonal antibodies could be stored at room temperature for as long as 30 days without detectable changes in antibody response when used for typing outer membrane antigens of N. meningitidis B.
De-Gaspari
E N
EN
Secão de Immunologia, Instituto Adolfo Lutz, São Paulo, Brasil.
Ribeiro-Filho
A A
AA
Zollinger
W D
WD
D000911:Antibodies, Monoclonal; D015373:Bacterial Typing Techniques; D015151:Immunoblotting / Q000379:methods*; D009345:Neisseria meningitidis / Q000145:classification*
D000911:Antibodies, Monoclonal