Heterogeneous localization of epitopes along axonemes of mammalian cilia.
The specificity of four monoclonal antibodies, raised against mammalian ciliary axonemes, was determined by both immunofluorescence and immunoblot experiments. Three antibodies reacted with epitopes which are differentially located along axonemal length. Among these, antibody 3.12 recognized an epitope common to different dynein heavy chains, reacted only with tracheal cilia and specifically stained the proximal portion of the ciliary axoneme.
Mencarelli
C
C
Dipartimento Biologia Evolutiva, Università di Siena, Italy.
Tiezzi
A
A
Ruggiero
P
P
Contorni
M
M
Massone
A
A
Moscatelli
A
A
Pallini
V
V
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D002417:Cattle; D002923:Cilia / Q000737:chemistry / Q000276:immunology* / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008970:Molecular Weight; D014132:Trachea / Q000648:ultrastructure
D000911:Antibodies, Monoclonal