Glial cells of the lamprey nervous system contain keratin-like proteins.
Lamprey axons regenerate following spinal cord transection despite the formation of a glial scar. As we were unable to detect a lamprey homologue of glial fibrillary acidic protein (GFAP), a major constituent of astrocytes, we studied the composition of intermediate filament (IF) proteins of lamprey glia. Monoclonal antibodies (mAbs) were raised to lamprey spinal cord cytoskeletal extracts and these mAbs were characterized by using Western blotting and immunocytochemistry. On two-dimensional (2-D) Western blots, five of the mAbs detected three major IF polypeptides in the molecular weight (MW) range of 45-56 kD. Further studies were conducted to determine the relationship between the lamprey glial-specific antigen and other mammalian IF proteins. Antikeratin 8 antibody recognized two of the three polypeptides. Several of the glial-specific mAbs reacted with human keratins 8 and 18 on Western blots. Keratin-like immunoreactivity was found in all parts of the central and peripheral nervous systems in both larval and adult lampreys. The immunocytochemical staining patterns of glial-specific mAbs were indistinguishable on lamprey spinal cord sections. However, on brain sections, two distinct patterns were observed. A subset of mAbs stained only a few glial fibers in the brain, whereas others stained many more brain glia, particularly the ependymal cells. The former group of mAbs recognized only the two lower MW polypeptides on 2-D Western blots, but the latter group of mAbs recognized all three major IF polypeptides. This correlation is supported by the observation that the highest MW IF polypeptide has an increased level of expression in the brain relative to the spinal cord. Thus, in the lamprey, the glial cells of both spinal cord and brain express molecules similar to simple epithelial cytokeratins, but their IFs may contain these keratins in different stoichiometric proportions. The widespread presence in the lamprey of primitive glial cells containing keratin-like intermediate filaments may have significance for the extraordinary ability of lamprey spinal axons to regenerate.
Merrick
S E
SE
David Mahoney Institute of Neurological Sciences, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104, USA.
Pleasure
S J
SJ
Lurie
D I
DI
Pijak
D S
DS
Selzer
M E
ME
Lee
V M
VM
D000818:Animals; D000911:Antibodies, Monoclonal; D001369:Axons / Q000737:chemistry; D001921:Brain / Q000166:cytology; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis*; D007798:Lampreys; D008854:Microscopy, Electron; D009420:Nervous System / Q000166:cytology*; D009457:Neuroglia / Q000166:cytology*; D013116:Spinal Cord / Q000166:cytology
D000911:Antibodies, Monoclonal; D007633:Keratins