title: Cytofluorometric evidence for differential genome endoreplication in the cluster neurons of Lophius piscatorius L. (Osteichthyes, Lophiiformes).
abstract: Quantitative microfluorometric evaluation indicated that, in Lophius piscatorius, the DNA content of large neurons of a cluster located at the boundary between the medulla oblongata and the spinal cord varied from 4C to more than 5000C. This finding must be considered exceptional for the nervous system of Vertebrates. DNA contents were correlated to both nuclear and animal size. Utilization of AT and GC specific fluorochromes showed that the increase in DNA content is due to differential genome amplification involving GC-rich DNA sequences.
authors.affiliation: Dipartimento di Biologia Animale, Università di Modena, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Sassi
authors.forename: G C
authors.initials: GC
authors.lastname: Manicardi
authors.forename: L
authors.initials: L
authors.lastname: Mola
authors.forename: I
authors.initials: I
authors.lastname: Benedetti
mesh_terms: D000818:Animals; D001482:Base Composition; D004247:DNA / Q000032:analysis; D004261:DNA Replication; D005399:Fishes / Q000235:genetics*; D008526:Medulla Oblongata / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D009697:Nucleolus Organizer Region / Q000737:chemistry / Q000648:ultrastructure; D016622:Silver Staining; D013116:Spinal Cord / Q000737:chemistry / Q000378:metabolism
chemical_list: D004247:DNA