Graft versus host disease with mixed chimerism.
We report a patient with graft versus host disease (GVHD) with mixed chimerism (MC). The patient had chronic myelogenous leukemia and received bone marrow transplantation (BMT) from his elder sister. Eighty days after BMT, erythematous lesions appeared on his chest. Histological examination from the skin lesion revealed lymphocytic infiltration into the upper dermis. Eosinophilic necrotic keratinocytes were scattered through the epidermis. Liquefaction degeneration was also recognized. Sicca syndrome appeared from 110 days after BMT. Detection of host origin Y-chromosome-specific DNA by polymerase chain reaction (PCR) method in bone marrow and peripheral blood showed that all bone marrow samples obtained 6 months from BMT were positive for Y-specific DNA, while peripheral blood became positive in the 60th month after BMT. The host origin normal karyotype (46,XY) in the bone marrow samples was identified for the first time in the 60th month after BMT. These results indicate that host-origin hematopoietic cells survived after BMT.
Morita
H
H
Department of Dermatology, Hyogo College of Medicine, Hyogo, Japan.
Kohno
J
J
Kitano
Y
Y
D000328:Adult; D016026:Bone Marrow Transplantation; D006086:Graft vs Host Disease / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D012867:Skin / Q000473:pathology; D018183:Transplantation Chimera