Expression of human plasminogen activator inhibitor-2 in the baculovirus expression system.
A cDNA coding for human plasminogen activator inhibitor-2 (PAI-2) was transferred into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus synthesized a 43-kDa PAI-2 protein, the majority of which was secreted. The recombinant PAI-2 was identical to native PAI-2 purified from human placenta with respect to interaction with polyclonal antibody and inhibition of urokinase-type plasminogen activator.
Pei
Y
Y
Department of Biochemistry, Nanjing University, P. R. China.
Zhou
A
A
Cao
X
X
Xu
X
X
D000818:Animals; D016367:Baculoviridae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D017396:Plasminogen Activator Inhibitor 2 / Q000378:metabolism* / Q000502:physiology; D018411:Spodoptera / Q000166:cytology / Q000821:virology
D017396:Plasminogen Activator Inhibitor 2