Expression of cell surface markers and myelin proteins in cultured oligodendrocytes from neonatal brain of rat and mouse: a comparative study.
Dissociated brain cell cultures are a useful model for investigating development and differentiation of oligodendrocytes in vitro. The current studies compare the developmental patterns of expression for oligodendrocyte lineage/myelin markers in both primary and secondary oligodendrocyte cultures derived from mouse and rat neonates. The rat and mouse dissociated brain cell cultures express the same myelin-specific antigens, but mouse oligodendrocytes produce a larger and more elaborate sheet-like membrane than rat oligodendrocytes, and some of the myelin markers (O4, GC, and MBP) show more intense membrane staining in mouse cultures. GD3 appears to be a good oligodendrocyte marker for rat cells, but it is nonspecific in mouse cells. There are fewer oligodendrocytes in mouse cultures, and they appear to require a longer differentiation time than rat oligodendrocytes. These same results are also observed in secondary oligodendrocyte cultures, although in general late myelin markers such as MBP and PLP are expressed at a much lower level in mouse cells than rat cells.
Duchala
C S
CS
Department of Psychiatry and Biobehavioral Science, UCLA Medical Center 90024, USA.
Asotra
K
K
Macklin
W B
WB
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology / Q000378:metabolism*; D000954:Antigens, Surface / Q000276:immunology*; D015153:Blotting, Western; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D019085:Fluorescent Antibody Technique, Direct; D051379:Mice; D009185:Myelin Proteins / Q000096:biosynthesis*; D009457:Neuroglia / Q000378:metabolism; D009836:Oligodendroglia / Q000276:immunology / Q000378:metabolism*; D051381:Rats
D000954:Antigens, Surface; D009185:Myelin Proteins