title: Dynamic and differential Oct-1 expression during early Xenopus embryogenesis: persistence of Oct-1 protein following down-regulation of the RNA.
abstract: As a first step towards the elucidation of the role of the transcription factor Oct-1 in development, we prepared a monoclonal antibody to study the spatio-temporal distribution of Oct-1 protein in vivo. Here we report differential expression of the Oct-1 gene in the Xenopus embryo both at the RNA and the protein level. Transcripts and protein are detected in ectodermal and mesodermal cell lineages, in which the expression exhibits a pattern of progressive spatial restriction in the course of development. The Oct-1 expression as reported here is not correlated with cell density or cell proliferation in the embryo. Our results suggest a role of Oct-1 in the specification and differentiation of neuronal and neural crest cells. In many other cells, the developmental decision to down regulate Oct-1 is delayed, probably due to a high stability of the protein.
authors.affiliation: Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
authors.forename: G J
authors.initials: GJ
authors.lastname: Veenstra
authors.forename: T L
authors.initials: TL
authors.lastname: Beumer
authors.forename: J
authors.initials: J
authors.lastname: Peterson-Maduro
authors.forename: B I
authors.initials: BI
authors.lastname: Stegeman
authors.forename: H A
authors.initials: HA
authors.lastname: Karg
authors.forename: P C
authors.initials: PC
authors.lastname: van der Vliet
authors.forename: O H
authors.initials: OH
authors.lastname: Destrée
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002455:Cell Division; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015536:Down-Regulation; D004475:Ectoderm / Q000166:cytology; D004625:Embryo, Nonmammalian / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; D012313:RNA / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D029867:Xenopus Proteins; D014982:Xenopus laevis
chemical_list: D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D051863:Host Cell Factor C1; D050811:Octamer Transcription Factor-1; C494169:POU2F1 protein, Xenopus; D014157:Transcription Factors; D029867:Xenopus Proteins; D012313:RNA