Porcine E-selectin: cloning and functional characterization.
E-selectin, a member of the selectin family, is believed to play an important role in mediating the initial adhesive events between leucocytes and the endothelium during inflammation. A monoclonal antibody against human E-selectin was found to cross-react with the porcine equivalent, a glycoprotein of 92,000 MW. Isolation of the cDNA for porcine E-selectin showed that it was 71% homologous with human E-selectin but had two less short consensus repeats. The porcine adhesion molecule could also support the adhesion of both porcine and human neutrophils. Expression of E-selectin on interleukin-1 alpha (IL-1 alpha)- or tumour necrosis factor-alpha (TNF-alpha)-activated porcine aortic endothelial cells in culture was prolonged, persisting for up to 48 hr. Binding studies using a chimeric molecule consisting of the lectin domain of porcine E-selectin and the epidermal growth factor (EGF) domain of human E-selectin fused to the human IgG constant region, further characterized porcine E-selectin as recognizing mainly granulocytic leucocytes and a subpopulation of lymphocytes.
Tsang
Y T
YT
Celltech Research, Celltech Ltd, Slough, Berkshire, UK.
Stephens
P E
PE
Licence
S T
ST
Haskard
D O
DO
Binns
R M
RM
Robinson
M K
MK
D000818:Animals; D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D002478:Cells, Cultured; D003429:Cross Reactions; D018076:DNA, Complementary / Q000235:genetics; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000276:immunology; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology; D007700:Kinetics; D007962:Leukocytes / Q000276:immunology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D013045:Species Specificity; D013552:Swine / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D019040:E-Selectin; D007375:Interleukin-1; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha