title: Capsular polysaccharide expressed by Pasteurella piscicida grown in vitro.
abstract: Pasteurella piscicida grown in a glucose-rich medium produces a capsule that can be see under light and electron microscopy. The capsular polysaccharide was purified and characterized by chemical and HPLC analysis. The polymer has the composition glucose/mannose/N-acetylgalactosamine/galacturonic acid/acetic acid in the molar ratios of approximately 2.5:1.3:0.5:0.4:2.5. The polysaccharide was immunogenic in rabbits and did not cross-react with antibodies against the O-antigen lipopolysaccharide.
authors.affiliation: Department de Microbiologia i Parasitologia Sanitàries, Facultat de Farmàcia, Universitat de Barcelona, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Bonet
authors.forename: B
authors.initials: B
authors.lastname: Magariños
authors.forename: J L
authors.initials: JL
authors.lastname: Romalde
authors.forename: M D
authors.initials: MD
authors.lastname: Simon-Pujol
authors.forename: A E
authors.initials: AE
authors.lastname: Toranzo
authors.forename: F
authors.initials: F
authors.lastname: Congregado
mesh_terms: D000818:Animals; D016667:Bacterial Capsules / Q000737:chemistry* / Q000648:ultrastructure; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D007106:Immune Sera; D008854:Microscopy, Electron; D019081:O Antigens; D010325:Pasteurella / Q000737:chemistry* / Q000254:growth & development / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits
chemical_list: D007106:Immune Sera; D019081:O Antigens; D011135:Polysaccharides, Bacterial