title: Kinetics of tyrosine phosphorylation when IgE dimers bind to FC epsilon receptors on rat basophilic leukemia cells.
abstract: Previously, we demonstrated that aggregates of the high affinity receptor for IgE (Fc epsilon RI), formed by the binding of chemically cross-linked oligomers of IgE, continue to signal early and late cellular responses long after the formation of new aggregates is blocked. In the present work, we explore quantitatively the relationship between aggregation of the receptors and one of the earliest biochemical changes this initiates. We compare the time course of aggregate formation, inferred from studies of the binding of dimers of IgE, and the time course of phosphorylation of tyrosines on receptor subunits when the receptors are aggregated. A simple model does not fit the data. It appears that aggregates formed late in the response are less effective signaling units than those formed initially. We propose new explanations for the persistence of the response and the unusual kinetics.
authors.affiliation: Department of Mathematics and Statistics, University of New Mexico, Albuquerque 87131, USA.
authors.forename: C
authors.initials: C
authors.lastname: Wofsy
authors.forename: U M
authors.initials: UM
authors.lastname: Kent
authors.forename: S Y
authors.initials: SY
authors.lastname: Mao
authors.forename: H
authors.initials: H
authors.lastname: Metzger
authors.forename: B
authors.initials: B
authors.lastname: Goldstein
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D007073:Immunoglobulin E / Q000378:metabolism*; D015471:Leukemia, Basophilic, Acute; D046911:Macromolecular Substances; D008433:Mathematics; D051379:Mice / Q000276:immunology; D008961:Models, Structural; D008962:Models, Theoretical; D010766:Phosphorylation; D019000:Phosphotyrosine; D011817:Rabbits; D051381:Rats; D017455:Receptors, IgE / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D046911:Macromolecular Substances; D017455:Receptors, IgE; D019000:Phosphotyrosine; D007073:Immunoglobulin E; D014443:Tyrosine