title: Visualisation of domains in the avian tectorial and otolithic membranes with monoclonal antibodies.
abstract: The staining patterns observed with six monoclonal antibodies (mAbs) raised in vitro against a fraction derived from the utricular macula were examined with cryosections of the auditory and vestibular organs of the avian inner ear. These antibodies revealed several distinct domains within the gelatinous membranes. Three different labelling patterns were observed in the tectorial membrane. Staining was seen either throughout the entire tectorial membrane, restricted to its core, or in a narrow zone lying close to the surface of the basilar papilla. In the maculae, the mAbs stained either the striolar region of the otolithic membrane or the entire structure. One monoclonal which labelled otoconia, stained small otoconia in their entirely, whilst larger otoconia were only labelled around their periphery. Only one of the mAbs stained the cupulae of the semi-circular canal ampullae and this antibody stained neither the tectorial nor the otolithic membranes. These results suggest that there are biochemically distinct regions in the gelatinous membranes of the inner ear and indicate that these matrices are not simply homogeneous extracellular structures.
authors.affiliation: School of Biological Sciences, University of Sussex, Falmer, Brighton, UK.
authors.forename: R
authors.initials: R
authors.lastname: Goodyear
authors.forename: M
authors.initials: M
authors.lastname: Holley
authors.forename: G
authors.initials: G
authors.lastname: Richardson
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001489:Basilar Membrane / Q000648:ultrastructure; D002645:Chickens; D003051:Cochlea / Q000502:physiology / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000378:metabolism / Q000502:physiology; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005629:Frozen Sections; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D010037:Otolithic Membrane / Q000033:anatomy & histology* / Q000276:immunology; D013194:Staining and Labeling; D013680:Tectorial Membrane / Q000033:anatomy & histology* / Q000276:immunology; D014909:Wheat Germ Agglutinins / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D014909:Wheat Germ Agglutinins; D016650:Fluorescein-5-isothiocyanate