title: Regulation of intracellular pH in the colonic epithelial cell line HT29 clone 19A.
abstract: Intracellular pH (pHi) of the colonic tumor cell line HT29 cl. 19A was studied by microspectrofluorometry using the pH-sensitive dye BCECF. Single cells within a confluent monolayer grown in a polarized manner on permeable supports were examined. An amiloride-sensitive Na(+)-H+ exchange and a stilbene-insensitive Cl(-)-HCO3- exchange mechanism have been identified in the basolateral membrane. This Na(+)-H+ exchange mechanism in the basolateral membrane is an acid extruder, whereas the Cl(-)-HCO3- exchanger is an acid loader. Both of these in the basolateral membrane located mechanisms are important for the maintenance of intracellular pH in HT29 cl. 19A cells.
authors.affiliation: Department of Physiology, School of Veterinary Medicine Hannover.
authors.forename: R
authors.initials: R
authors.lastname: Busche
authors.forename: J
authors.initials: J
authors.lastname: Bartels
authors.forename: W
authors.initials: W
authors.lastname: von Engelhardt
mesh_terms: D017920:Antiporters / Q000378:metabolism*; D001639:Bicarbonates / Q000378:metabolism; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides / Q000378:metabolism; D003106:Colon / Q000378:metabolism*; D003110:Colonic Neoplasms / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured
chemical_list: D017920:Antiporters; D001639:Bicarbonates; D027963:Chloride-Bicarbonate Antiporters; D002712:Chlorides