Strategies for studying mouse and human immune responses to human papillomavirus type 16.
Cytotoxic T lymphocytes (CTL) are an important protective mechanism in viral infection and can be effective against tumours. We have investigated the tumour-associated E6 and E7 genes of human papillomavirus type 16 as CTL targets. In H-2b mice we have defined epitopes in E6 and E7 which can readily generate CTL in vivo and we have shown that HLA-A2.1 transgenic mice can generate an HLA-A2.1-restricted response. We have been unable to reveal a primed CTL response in humans. These paradoxical findings imply that human papillomavirus may fail to stimulate a systemic CTL response and/or employ strategies for evading or down-regulating such a response.
Beverley
P C
PC
ICRF Tumour Immunology Unit, University College London Medical School, UK.
Sadovnikova
E
E
Zhu
X
X
Hickling
J
J
Gao
L
L
Chain
B
B
Collins
S
S
Crawford
L
L
Vousden
K
K
Stauss
H J
HJ
D000595:Amino Acid Sequence; D000818:Animals; D000939:Epitopes / Q000276:immunology; D005814:Genes, Viral; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014616:Vaccinia virus; D014765:Viral Vaccines / Q000276:immunology*
D000939:Epitopes; D015789:HLA-A2 Antigen; D014614:Vaccines, Synthetic; D014765:Viral Vaccines