title: Immunocytochemical characterization of explant cultures of human prostatic stromal cells.
abstract: The study of stromal-epithelial interactions greatly depends on the ability to culture both cell types separately, in order to permit analysis of their interactions under defined conditions in reconstitution experiments. Here we report the establishment of explant cultures of human prostatic stromal cells and their immunocytochemical characterization. As determined by antibodies to keratin and prostate specific acid phosphatase, only small numbers (< 5%) of epithelial cells were present in primary cultures; subsequent passaging further reduced epithelial cell contamination. Antibodies against intermediate filament proteins (keratins, vimentin, and desmin) and smooth muscle actin microfilaments demonstrated that stromal cells from benign prostatic hyperplasia and prostate carcinoma differed in regard to their differentiation markers. Two contrasting phenotypes were identified in cultures derived from these two different lesions: One exhibiting fibroblastic features, was predominant in cultures derived from benign lesions and a second, showing varying degrees of smooth muscle differentiation, was more abundant in carcinoma-derived cultures. These findings are indicative of a remarkable divergence in the stromal-epithelial relationships associated with these pathological conditions and may provide us with a potential tool for studying these processes.
authors.affiliation: Department of Urology, Erasmus University, Academic Hospital Dijkigt, Rotterdam, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Kooistra
authors.forename: N M
authors.initials: NM
authors.lastname: Elissen
authors.forename: J J
authors.initials: JJ
authors.lastname: König
authors.forename: M
authors.initials: M
authors.lastname: Vermey
authors.forename: T H
authors.initials: TH
authors.lastname: van der Kwast
authors.forename: J C
authors.initials: JC
authors.lastname: Romijn
authors.forename: F H
authors.initials: FH
authors.lastname: Schröder
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000199:Actins / Q000032:analysis; D015415:Biomarkers / Q000032:analysis*; D003893:Desmin / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis; D008297:Male; D011467:Prostate; D011470:Prostatic Hyperplasia / Q000378:metabolism* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D011944:Receptors, Androgen / Q000032:analysis; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis
chemical_list: D000199:Actins; D015415:Biomarkers; D003893:Desmin; D011944:Receptors, Androgen; D014746:Vimentin; D007633:Keratins; D000135:Acid Phosphatase