Flow cytometric determination of pre-transfusion red cell volume in fetuses and neonates requiring transfusion based on RhD+ dilution by transfused D- red cells.
We report a case of feto-maternal haemorrhage and describe a new flow-cytometric method of determining a fetus's or infant's pre-transfusion red cell volume (RCV). We validate this method against an established technique, employing fetal haemoglobin (HbF) dilution, for determining the RCV in fetuses and neonates requiring intravascular transfusion. We discuss advantages and other potential applications of this new method.
Wynn
R
R
Department of Haematology, University Hospital of Wales, Cardiff.
Dixon
S
S
al-Ismail
S A
SA
Jones
J G
JG
Fisher
J
J
Hoy
T
T
Dawson
A
A
Wardrop
C A
CA
D001805:Blood Transfusion, Intrauterine; D017707:Erythrocyte Transfusion; D004911:Erythrocyte Volume; D005260:Female; D005319:Fetal Hemoglobin / Q000032:analysis; D005331:Fetomaternal Transfusion / Q000097:blood* / Q000628:therapy; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D007518:Isoantibodies / Q000097:blood*; D011247:Pregnancy
D007518:Isoantibodies; C033242:RH-antibodies; D005319:Fetal Hemoglobin