Identification and characterization of sporulation gene spoVS from Bacillus subtilis.
We report the identification and characterization of an additional sporulation gene from Bacillus subtilis called spoVS, which is induced early in sporulation under the control of sigma H. We show that spoVS is an 86-codon-long open reading frame and is capable of encoding a protein of 8,796 Da which exhibits little similarity to other proteins in the databases. Null mutations in spoVS have two contrasting phenotypes. In otherwise wild-type cells they block sporulation at stage V, impairing the development of heat resistance and coat assembly. However, the presence of a spoVS mutation in a spoIIB spoVG double mutant (which is blocked at the stage [II] of polar septation) acts as a partial suppressor, allowing sporulation to advance to a late stage. The implications of the contrasting phenotypes are discussed in the context of the formation and maturation of the polar septum.
Resnekov
O
O
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Driks
A
A
Losick
R
R
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D016153:Genes, Suppressor / Q000235:genetics; D006358:Hot Temperature; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010641:Phenotype; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000502:physiology; D013171:Spores, Bacterial / Q000235:genetics / Q000648:ultrastructure
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D012808:Sigma Factor; C095877:SpoIIB protein, Bacillus subtilis; C095876:SpoVG protein, Bacillus subtilis; C095902:SpoVS protein, Bacillus subtilis