Characterisation of antibodies specific for chick brain neural cell adhesion molecules which cause amnesia for a passive avoidance task.
Antisera were prepared against six postsynaptic density glycoprotein fractions (150-180, 62-80, 50, 41, 33, and 28 kDa) that show enhanced fucosylation during memory formation after training day-old chicks in a one-trial passive avoidance task. Each antiserum was tested for its possible effect on memory retention. Bilateral intracranial injections of two of the antisera, R-1 and R-6, or their IgGs (IgG-1 and IgG-6), resulted in amnesia for the passive avoidance task when chicks were tested 24 h later, IgG-1 and IgG-6 antibodies were amnestic only when injected 5.5 h after training, and had no effect when injections were made 30 min before training, thus resembling an effect previously observed with polyclonal or monoclonal anti-N-CAM antibodies. IgG-1 and IgG-6 antibodies were found to be specific for protein epitopes of glycoproteins that contain a high amount of N-linked mannose and fucose, and a very low amount of polysialic acid and O-linked galactose. Absorption of IgG-6 antibodies with neural cell adhesion molecule (N-CAM) isolated from synaptic plasma membranes derived from day-old chick brain resulted in loss of amnestic effect.(ABSTRACT TRUNCATED AT 250 WORDS)
Mileusnic
R
R
Biology Department, Open University, Milton Keynes, England.
Rose
S P
SP
Lancashire
C
C
Bullock
S
S
D000647:Amnesia / Q000139:chemically induced; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001362:Avoidance Learning / Q000502:physiology*; D001921:Brain / Q000378:metabolism* / Q000502:physiology; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry / Q000276:immunology / Q000502:physiology*; D002645:Chickens; D000939:Epitopes; D005260:Female; D006023:Glycoproteins / Q000276:immunology / Q000502:physiology; D007074:Immunoglobulin G / Q000145:classification / Q000276:immunology; D007267:Injections; D007536:Isomerism; D008297:Male; D008565:Membrane Proteins / Q000276:immunology / Q000502:physiology; D008568:Memory / Q000502:physiology*; D012153:Retention, Psychology / Q000187:drug effects; D013569:Synapses / Q000378:metabolism
D000906:Antibodies; D015816:Cell Adhesion Molecules, Neuronal; D000939:Epitopes; D006023:Glycoproteins; D007074:Immunoglobulin G; D008565:Membrane Proteins