title: PBDX is the XG blood group gene.
abstract: We have identified the Xga antigen, encoded by the XG blood group gene, by employing rabbit polyclonal and mouse monoclonal antibodies raised against a peptide derived from the N-terminal domain of a candidate gene, referred to earlier as PBDX. In indirect haemagglutination assays, these anti-peptide antibodies react with Xg(a+) but not Xg(a-) erythrocytes. In antibody-specific immobilization of antigen (ASIA) and immunoblot assays, the anti-peptide antibodies react with the same molecule as does human anti-Xga. Therefore, by its identity with PBDX, Xga is identified as a cell-surface protein that is 48% homologous to CD99 (previously designated the 12E7 antigen), the product of MIC2 which is tightly linked to XG. PBDX is renamed here XG.
authors.affiliation: New York Blood Center, New York 10021.
authors.forename: N A
authors.initials: NA
authors.lastname: Ellis
authors.forename: P
authors.initials: P
authors.lastname: Tippett
authors.forename: A
authors.initials: A
authors.lastname: Petty
authors.forename: M
authors.initials: M
authors.lastname: Reid
authors.forename: P A
authors.initials: PA
authors.lastname: Weller
authors.forename: T Z
authors.initials: TZ
authors.lastname: Ye
authors.forename: J
authors.initials: J
authors.lastname: German
authors.forename: P N
authors.initials: PN
authors.lastname: Goodfellow
authors.forename: S
authors.initials: S
authors.lastname: Thomas
authors.forename: G
authors.initials: G
authors.lastname: Banting
mesh_terms: D000071443:12E7 Antigen; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD; D001483:Base Sequence; D001789:Blood Group Antigens / Q000235:genetics*; D015153:Blotting, Western; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D003429:Cross Reactions; D000939:Epitopes / Q000276:immunology; D005347:Fibroblasts / Q000378:metabolism; D005796:Genes; D008040:Genetic Linkage; D006386:Hemagglutination Tests; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
chemical_list: D000071443:12E7 Antigen; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D001789:Blood Group Antigens; C061828:CD99 protein, human; D015815:Cell Adhesion Molecules; D000939:Epitopes; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; C088785:XG protein, human