title: Changes in radiation sensitivity of human osteosarcoma cells after p53 introduction.
abstract: Human osteosarcoma SAOS-2 cells, which have a deletion in p53 gene, were transfected with plasmid pMSVneop53 containing human p53 cDNA and neomycin-resistance gene. Three clones (SAOS-MC10, SAOS-MC11 and SAOS-MC43) among 60 clones expressed p53 mRNA. No p53 protein was observed in SAOS-MC10, while SAOS-MC11 and SAOS-MC43 produced p53 protein. The molecular weight of p53 protein in SAOS-MC43 was lower than that in SAOS-MC11, SAOS-MC11 and SAOS-MC43 were more sensitive and more resistant, respectively, to ionizing radiation than the parental SAOS-2. We suggest that exogenous p53 protein might be one of the factors determining cellular radiosensitivity.
authors.affiliation: Department of Radiation Genetics, Faculty of Medicine, Kyoto University.
authors.forename: J
authors.initials: J
authors.lastname: Miyakoshi
authors.forename: N
authors.initials: N
authors.lastname: Yamagishi
authors.forename: S
authors.initials: S
authors.lastname: Ohtsu
authors.forename: H
authors.initials: H
authors.lastname: Takebe
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000532:radiotherapy; D016158:Genes, p53 / Q000502:physiology*; D006801:Humans; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology* / Q000532:radiotherapy; D011836:Radiation Tolerance; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000528:radiation effects