Human histocompatibility leukocyte antigen-DR and heat shock protein-70 expression in eye muscle tissue in thyroid-associated ophthalmopathy.
To investigate the expression and localization of human leukocyte antigen (HLA)-DR and heat shock protein-70 (HSP-70) in orbital tissue from patients with thyroid-associated ophthalmopathy (TAO), we carried out an immunohistochemical study using anti-HLA-DR and anti-HSP-70 monoclonal antibodies and a streptavidin-biotinperoxidase detection system. Eye muscle tissues were obtained at surgery from 38 patients with TAO and 8 control subjects. HLA-DR expression on eye muscle cells was demonstrated in orbital tissue from 2 of 3 untreated patients and 2 of 35 patients who had been treated with orbital irradiation or corticosteroids, in all of whom lymphocytic infiltration was also demonstrated. HLA-DR was not detected on eye muscle cells from 8 normal controls studied. HLA-DR was expressed on endothelial cells and interstitial cells from almost all patients with TAO and all 8 control subjects. HSP-70 was detected in eye muscle cells from 31 of the patients with TAO, including all 3 untreated patients, and 3 of the controls. Although the degree of HSP-70 expression did not correlate with the severity of the ophthalmopathy, significant expression of HSP-70 in eye muscle cells was more often demonstrated in patients with eye disease of short duration (83%) than in those with disease of longer duration (33%). These results support the notion that eye muscle fiber is an important target of the orbital autoimmune reactions that characterize TAO.
Hiromatsu
Y
Y
Department of Medicine, Kurume University School of Medicine, Japan.
Tanaka
K
K
Ishisaka
N
N
Kamachi
J
J
Kuroki
T
T
Hoshino
T
T
Inoue
Y
Y
Wall
J R
JR
Nonaka
K
K
D000328:Adult; D000368:Aged; D005128:Eye Diseases / Q000209:etiology* / Q000378:metabolism* / Q000628:therapy; D005260:Female; D006684:HLA-DR Antigens / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009801:Oculomotor Muscles / Q000378:metabolism* / Q000473:pathology; D013194:Staining and Labeling; D013959:Thyroid Diseases / Q000150:complications*
D006684:HLA-DR Antigens; D018840:HSP70 Heat-Shock Proteins