Mechanical induction of beta 1-integrin-mediated calcium signaling in a hepatocyte cell line.
Mechanical stress influences growth, differentiation, and gene expression in a variety of cell types. It is believed that via extracellular matrix the mechanical stimulus is transmitted to integrin receptors which thus play a key role in transducing signals into the cell interior. Here we demonstrate that incubation of suspended hepatocytes with specific antibodies to beta 1-integrin subunits followed by a short-term mechanical stimulation is sufficient to induce a rise in intracellular Ca2+. The results indicate that mechanical loading of individual integrin subunits activates Ca(2+)-specific signal pathways.
Nebe
B
B
Department of Internal Medicine, University of Rostock, Germany.
Rychly
J
J
Knopp
A
A
Bohn
W
W
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D019012:Integrin beta1; D016023:Integrins / Q000276:immunology / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D051379:Mice; D015398:Signal Transduction; D013314:Stress, Mechanical
D000911:Antibodies, Monoclonal; D019012:Integrin beta1; D016023:Integrins; D002118:Calcium