Effect of cyclosporin A on energy coupling in pea stem mitochondria.
Effect of cyclosporin A on energy coupling in pea stem mitochondria is studied. It is found that incubation of mitochondria with 100 nM FCCP and/or CAtr, oligomycin, CaCl2, palmitate and ADP results, after some lag phase, in a collapse of delta psi generated by succinate oxidation in the presence of rotenone. Cyclosporin A (0.2-0.8 nmol/mg mitochondrial protein) markedly increases the lag phase. The cyclosporin A effect requires dithioerythritol to be added to the isolated medium. Metabisulphite fails to substitute for dithioerythritol. The relationships between these effects and cyclosporin A-sensitive mitochondrial permeability transition in animal mitochondria are discussed.
Vianello
A
A
Department of Biology and Agro-industrial Economics, University of Udine, Italia.
Macri
F
F
Braidot
E
E
Mokhova
E N
EN
D000818:Animals; D016572:Cyclosporine / Q000494:pharmacology*; D004226:Dithioerythritol / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D018532:Pisum sativum / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D014475:Uncoupling Agents / Q000494:pharmacology
D014475:Uncoupling Agents; D004226:Dithioerythritol; D016572:Cyclosporine