Interleukin 6 mediated differentiation and rescue of cell redox in PC12 cells exposed to ionizing radiation.
The differentiation of PC12 cells to a neuron-like morphology was induced by ionizing radiation in the presence of serum. This effect was detectable at 5 grays (Gy) and reached a maximum at 10-20 Gy. Increases in the DNA binding activity of nuclear factor kappa B (NF-kappa B) and increased Interleukin 6 (IL-6) mRNA levels were observed at a dose of 15 Gy. Neutralization of supernatant IL-6 by the addition of anti-IL-6 antibody inhibited the neuronal differentiation and decreased cellular redox. Ionizing radiation and serum may act synergistically as neurotropic factors.
Abeyama
K
K
Department of Laboratory Medicine, Faculty of Medicine, Kagoshima University, Japan.
Kawano
K
K
Nakajima
T
T
Takasaki
I
I
Kitajima
I
I
Maruyama
I
I
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001483:Base Sequence; D002454:Cell Differentiation / Q000502:physiology*; D015870:Gene Expression / Q000528:radiation effects; D015850:Interleukin-6 / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism / Q000528:radiation effects; D016501:Neurites / Q000502:physiology / Q000528:radiation effects; D010084:Oxidation-Reduction; D016716:PC12 Cells / Q000473:pathology / Q000528:radiation effects*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000737:chemistry; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D000093242:Interleukin-6 Inhibitors
D000906:Antibodies; D015850:Interleukin-6; D016328:NF-kappa B; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D000093242:Interleukin-6 Inhibitors