title: H19 expression and tumorigenicity of choriocarcinoma derived cell lines.
abstract: Certain embryonal tumors demonstrate a loss of heterozygosity at the parentally imprinted region of chromosome 11p15.5. It has been hypothesized that this implicates a tumor suppressor gene at this locus. The human H19 gene maps to 11p15.5, is expressed in fetal tissues including the placenta and is paternally imprinted. Here we show that the abundance of H19 transcripts in cells of two choriocarcinoma derived cell lines (JAr and JEG-3) differs greatly. While JAr cells express high levels of H19 RNA, the expression of H19 in JEG-3 cells is much lower than that of normal trophoblasts. Cells of these two cell lines were subcutaneously injected into nude mice with subsequent tumor formation. A fivefold increase in the H19 RNA level was measured in tumors derived from JEG-3 cell lines as compared to these cells before injection. However this increase in H19 RNA did not alter the clonogenicity in soft agar nor the growth rate of the cells derived from these tumors as compared to the original JEG-3 cells. Nevertheless, the cells retaining the elevated level of H19 transcripts were more tumorigenic than the original cells. We propose that there is a selection of cells expressing high levels of H19 from the total JEG-3 cell population during the microevolution of tumor formation. These observations, together with our previous publications on H19 expression in human cancers, do not support the notion of a tumor suppressor role for the H19 gene.
authors.affiliation: Department of Biological Chemistry, Silberman Institute of Life Sciences, Hebrew University, Jerusalem, Israel.
authors.forename: J
authors.initials: J
authors.lastname: Rachmilewitz
authors.forename: M
authors.initials: M
authors.lastname: Elkin
authors.forename: J
authors.initials: J
authors.lastname: Rosensaft
authors.forename: Z
authors.initials: Z
authors.lastname: Gelman-Kohan
authors.forename: I
authors.initials: I
authors.lastname: Ariel
authors.forename: O
authors.initials: O
authors.lastname: Lustig
authors.forename: T
authors.initials: T
authors.lastname: Schneider
authors.forename: R
authors.initials: R
authors.lastname: Goshen
authors.forename: H
authors.initials: H
authors.lastname: Biran
authors.forename: N
authors.initials: N
authors.lastname: de Groot
mesh_terms: D000818:Animals; D001483:Base Sequence; D002822:Choriocarcinoma / Q000235:genetics*; D006063:Chorionic Gonadotropin / Q000235:genetics; D018997:Chorionic Gonadotropin, beta Subunit, Human; D005260:Female; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics*; D010446:Peptide Fragments / Q000235:genetics; D011247:Pregnancy; D011519:Proto-Oncogenes; D012313:RNA / Q000032:analysis; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D012878:Skin Neoplasms / Q000235:genetics; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; C094335:H19 long non-coding RNA; D009124:Muscle Proteins; D010446:Peptide Fragments; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D012313:RNA