title: New human oral squamous carcinoma cell line and its tumorigenic subline producing granulocyte colony-stimulating factor.
abstract: A new human carcinoma cell line, MISK81-5, was established from a metastatic lymph node of oral squamous cell carcinoma. Immunocytochemical and ultrastructural observations revealed an obvious epithelial origin of the cell line. Chromosome analysis revealed a hypertriploid karyotype with numerical and structural anomalies. MISK81-5 cells could form a tumor mass in the subcutaneous tissue of recipient BALB/c athymic mice only when coinjected with Matrigel. A stem cell assay revealed that conditioned medium (CM) of MISK81-5 contained granulocyte colony-stimulating factor (G-CSF) or interleukin-6 activity. Quantitation by ELISA disclosed a higher concentration of G-CSF in the CM of MISK81-5 than in the CM of other squamous and gastric carcinoma cell lines. The sMISK, that was derived from MISK81-5 as a subpopulation of the cell line having higher tumorigenicity, also showed a similar hematopoietic stimulating activity to that of MISK81-5. These characteristics of the MISK81-5 cell line and its subpopulation, sMISK will be useful for studying the biological behavior of oral squamous cell carcinomas and its relation to hematopoietic stimulating factors.
authors.affiliation: Department of Oral Pathology, Faculty of Dentistry, Kyushu University, Fukuoka.
authors.forename: K
authors.initials: K
authors.lastname: Matsuo
authors.forename: Y
authors.initials: Y
authors.lastname: Ishibashi
authors.forename: I
authors.initials: I
authors.lastname: Kobayashi
authors.forename: S
authors.initials: S
authors.lastname: Ozeki
authors.forename: M
authors.initials: M
authors.lastname: Ohishi
authors.forename: T
authors.initials: T
authors.lastname: Tange
authors.forename: J
authors.initials: J
authors.lastname: Hirata
authors.forename: T
authors.initials: T
authors.lastname: Kiyoshima
authors.forename: H
authors.initials: H
authors.lastname: Sakai
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D003043:Cocarcinogenesis; D003094:Collagen / Q000633:toxicity; D017077:Culture Media, Conditioned / Q000633:toxicity; D004338:Drug Combinations; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D007797:Laminin / Q000633:toxicity; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000378:metabolism*; D009368:Neoplasm Transplantation; D011509:Proteoglycans / Q000633:toxicity; D014407:Tumor Cells, Cultured / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D004338:Drug Combinations; D015850:Interleukin-6; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D016179:Granulocyte Colony-Stimulating Factor; D003094:Collagen