title: Lymphocytic Na(+)-H+ exchange increases after an oral glucose challenge.
abstract: The effects of oral glucose challenge on plasma glucose concentration, plasma insulin concentration, arterial blood pressure, cytosolic pH (pHi), cytosolic free Na+ concentration ([Na+]i), and cellular Na(+)-H+ exchange activity were investigated in 16 healthy subjects. The pHi, [Na+]i, and Na(+)-H+ exchange activity were measured in intact lymphocytes by using the fluorescent dye technique. The oral glucose challenge significantly increased plasma glucose, plasma insulin, and the lymphocytic Na(+)-H+ exchange activity, measured as change of pHi per second (control [0 hours], 5.20 +/- 0.53 x 10(-3) dpHi/s; 1 hour after glucose administration, 8.28 +/- 1.07 x 10(-3) dpHi/s; 2 hours after glucose administration, 8.15 +/- 1.18 x 10(-3) dpHi/s; P = .002). The lymphocytic Na(+)-H+ exchange was significantly correlated with plasma glucose concentration (r = .357, P = .041). During steady state euglycemic hyperinsulinemic clamp, the Na(+)-H+ exchange activity was not significantly changed compared with baseline values. The study shows that changes of blood glucose levels can induce an acute increase in Na(+)-H+ exchange activity. Systolic blood pressure and Na(+)-H+ exchange activity were significantly (P < .001) but weakly correlated during an oral glucose challenge.
authors.affiliation: Medizinische Universitäts-Poliklinik, Münster, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Tepel
authors.forename: R
authors.initials: R
authors.lastname: Schlotmann
authors.forename: M
authors.initials: M
authors.lastname: Barenbrock
authors.forename: K
authors.initials: K
authors.lastname: Kisters
authors.forename: T
authors.initials: T
authors.lastname: Klaus
authors.forename: C
authors.initials: C
authors.lastname: Spieker
authors.forename: M
authors.initials: M
authors.lastname: Walter
authors.forename: C
authors.initials: C
authors.lastname: Meyer
authors.forename: R G
authors.initials: RG
authors.lastname: Bretzel
authors.forename: W
authors.initials: W
authors.lastname: Zidek
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure; D005951:Glucose Tolerance Test; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000032:analysis; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D012964:Sodium / Q000097:blood* / Q000378:metabolism; D017923:Sodium-Hydrogen Exchangers / Q000097:blood* / Q000378:metabolism; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D007328:Insulin; D017923:Sodium-Hydrogen Exchangers; D012964:Sodium