Complete removal of mycoplasma from viral preparations using solvent extraction.
Mycoplasma contamination of two alphavirus isolates and adenovirus 5 was completely removed using a very simple, rapid protocol based on solvent extraction. Treated virus preparations remained free of mycoplasma after 6 months in culture.
La Linn
M
M
Queensland Institute of Medical Research, Bancroft Centre, Brisbane, Australia.
Bellett
A J
AJ
Parsons
P G
PG
Suhrbier
A
A
D000260:Adenoviruses, Human / Q000302:isolation & purification / Q000502:physiology*; D001431:Bacteriological Techniques; D006690:Bisbenzimidazole; D006367:HeLa Cells; D006801:Humans; D009174:Mycoplasma / Q000302:isolation & purification*; D012398:Ross River virus / Q000302:isolation & purification / Q000502:physiology*; D012997:Solvents; D014773:Virology / Q000379:methods
D012997:Solvents; D006690:Bisbenzimidazole