A mechanism for the specific immunogenicity of heat shock protein-chaperoned peptides.
Endogenously synthesized antigenic determinants are generally presented on major histocompatibility complex (MHC) class I molecules, whereas exogenous determinants are presented by MHC class II molecules. Here, it is shown that exogenous antigens chaperoned by a heat shock protein can be channeled into the endogenous pathway, presented by MHC class I molecules, and recognized by CD8+ T lymphocytes. This pathway is functional only in a subset of macrophages among the cell types tested. These observations provide a basis for the tumor-specific and virus-specific immunogenicity of cognate heat shock protein preparations and offer a mechanism for the classical phenomenon of cross-priming.
Suto
R
R
Department of Biological Sciences, Fordham University, Bronx, NY 10458, USA.
Srivastava
P K
PK
D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000951:Antigens, Neoplasm / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology*; D002478:Cells, Cultured; D018833:Chaperonins / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D000939:Epitopes; D015395:Histocompatibility Antigens Class I; D010455:Peptides; D014409:Tumor Necrosis Factor-alpha; C052646:sarcoma glycoprotein gp96 rejection antigens; D018833:Chaperonins