Surface morphology and chemical characterization of abrasion/erosion lesions.
OBJECTIVE
To describe the dentin surface morphology of abrasion/erosion lesions and to chemically characterize in vivo samples of sclerotic dentin.


METHODS
Baseline polyvinylsiloxane impressions of eight in vivo caries-free lesions were taken. Dentin was collected from retention grooves for FTIR photoacoustic spectroscopic analysis. The cavity preparation was etched for 30 seconds with 37% phosphoric acid, rinsed, and dried. Impressions were taken of the etched surfaces. Epoxy resin dies were made of baseline and etched impressions, sputter-coated and examined at x1000 with the SEM.


RESULTS
Lateral dentin tubule orientation was observed at gingival margins and on occlusal walls. Open cross-sectional tubules were seen at the depth of the groove. The mineral/protein ratio in the FTIR/PAS spectra of in vivo unetched sclerotic dentin samples suggested an increased mineral content.
Mixson
J M
JM
Department of Restorative Dentistry, University of Missouri-Kansas City, School of Dentistry 64108-2795, USA.
Spencer
P
P
Moore
D L
DL
Chappell
R P
RP
Adams
S
S
D000134:Acid Etching, Dental; D000368:Aged; D002114:Calcinosis / Q000473:pathology; D003804:Dentin / Q000737:chemistry / Q000648:ultrastructure; D003809:Dentin, Secondary / Q000737:chemistry* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D008903:Minerals / Q000032:analysis; D003765:Models, Dental; D010756:Phosphoric Acids; D011506:Proteins / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014072:Tooth Abrasion / Q000473:pathology*; D014077:Tooth Erosion / Q000473:pathology*
D008903:Minerals; D010756:Phosphoric Acids; D011506:Proteins; C030242:phosphoric acid