Improvement of the strain for the rapid identification of genes encoding restriction and modification enzymes.
The E. coli AP1-200-9 strain for rapid identification of genes encoding restriction and modification enzymes carries a temperature sensitive lacZ gene fused to the damage-inducible dinD locus. A derivative of this strain was constructed that has a wild-type form of this locus which allows for a more efficient identification of recombinant plasmids encoding restriction and modifications enzymes.
Piekarowicz
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Institute of Microbiology, Warsaw University, Poland.
Wegleńska
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D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D015254:DNA Modification Methylases / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D007763:Lac Operon / Q000235:genetics; D009154:Mutation; D010641:Phenotype; D010957:Plasmids / Q000235:genetics*; D013014:SOS Response, Genetics; D013696:Temperature
D001426:Bacterial Proteins; D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; C087483:dinD protein, E coli; D015254:DNA Modification Methylases; D004262:DNA Restriction Enzymes