Annexin 1 is overexpressed and specifically secreted during experimentally induced colitis in rats.
Annexin 1 is a protein induced by glucocorticoids endowed with extracellular anti-inflammatory properties. In this study, the local expression and secretion of annexins 1-6, in rat proximal colon, were studied at different times after intracolonic administration of 30 mg trinitrobenzenesulfonic acid in 50% ethanol. Secretion was identified by incubating colonic tissues in a culture medium. The expression of annexins was detected by immunoblotting in tissue homogenates and incubation media. Inflammatory stages were evaluated by measuring myeloperoxidase activity. Annexin 1 expression in colons increased after trinitrobenzenesulfonic acid treatment and was maximal between days 1 to 9, during the cellular stage of the inflammation that corresponded to maximal myeloperoxidase activity. From 12 h to 9 days after trinitrobenzenesulfonic acid/ethanol treatment, annexin 1 was specifically secreted. Annexin 3 was also overexpressed during the cellular stage, but the expression of annexins 2, 4, 5, and 6 was unchanged; none of these annexins were secreted. Annexin 1 was shown to be physiologically secreted because its release was specific, abundant, and not correlated with cellular lysis. Annexin 1 may be considered as a putative candidate in the control of the gut inflammatory processes.
Vergnolle
N
N
Department of Pharmacology, Institut National de la Recherche Agronomique, Toulouse, France.
Coméra
C
C
Buéno
L
L
D000818:Animals; D017305:Annexin A1 / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism*; D017302:Annexins / Q000096:biosynthesis; D003092:Colitis / Q000139:chemically induced / Q000503:physiopathology*; D005110:Extracellular Space / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014302:Trinitrobenzenesulfonic Acid / Q000633:toxicity
D017305:Annexin A1; D017302:Annexins; D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase