[Exploration on experimental conditions of dot-ELISA for Trichomonas vaginalis using intact parasite antigen-monoclonal antibody detecting system].
This paper deals with the effect of different experimental conditions including the number of parasite, blocking fluid, incubation time and different types of antigens on dot-ELISA for Trichomonas vaginalis. Five microlitres of freshly washed intact worms at a concentration of 7.7 x 10(5)-3 x 10(8) cells/ml were added onto nitrocellulose membrane blocked with 1% BSA, 10% bovine serum or milk power (0.1%, 0.25%, 0.5%, 5%). Dot-ELISA using nitrocellulose membrane containing whole cell antigen 2.5 x 10(7) cells/ml, blocked by 0.5% defatted milk power at 37 degrees C for one hour before the incubation in McAb, incubated at 37 degrees C for one hour in McAb and SPA respectively was found to give more promising results. The whole cell antigen could be preserved for four months at 4 degrees C and -20 degrees C.
Yu
C
C
Department of Parasitology, Beijing Medical University.
Gao
X
X
Zhu
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan / Q000032:analysis*; D000953:Antigens, Protozoan / Q000276:immunology*; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D014246:Trichomonas vaginalis / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan