title: The perinatal presence of antigen (p-azophenylarsonate) or anti-mu antibodies lead to the loss of the recurrent idiotype (CRIA) in A/J mice.
abstract: The immune response of A/J mice against p-azophenylarsonate (Ars)-keyhole limpet hemocyanin (KLH) is characterized by the dominance, late in primary and during the secondary, of a recurrent idiotype called CRIA, encoded by a canonical combination of Ig gene segments. In this study, A/J mice were given Ars coupled to deaggregated human gamma globulins (dHGG) within 24 h after delivery. The offsprings from these mice were then exposed as adults to Ars-KLH. These animals developed an unusual immune response. The level of anti-Ars antibodies was nearly normal but a dramatic shift in repertoire was observed: the cross-reactive idiotype which is the hallmark of the anti-Ars response in A/J mice was completely absent. The idiotype could be recovered by injection of anti-idiotypic antibodies alone, with no need of lipopolysaccharide coupling. Therefore the presence of antigen at birth can lead to a strong perturbation of idiotype selection. Similar results were obtained with neonatal treatment using anti-IgM antibodies. After recovery of suppression, A/J mice can mount an anti-arsonate response of normal level but devoid of the dominant idiotype.
authors.affiliation: Laboratoire de Physiologie Animale, Université Libre de Bruxelles, Rhode-saint-Genèse, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Ryelandt
authors.forename: D
authors.initials: D
authors.lastname: De Wit
authors.forename: A
authors.initials: A
authors.lastname: Baz
authors.forename: G
authors.initials: G
authors.lastname: Vansanten
authors.forename: O
authors.initials: O
authors.lastname: Denis
authors.forename: F
authors.initials: F
authors.lastname: Huetz
authors.forename: F
authors.initials: F
authors.lastname: Nisol
authors.forename: F
authors.initials: F
authors.lastname: Macedo-Soares
authors.forename: S
authors.initials: S
authors.lastname: Barcy
authors.forename: M
authors.initials: M
authors.lastname: Brait
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000915:Antibody Affinity; D007108:Immune Tolerance; D007130:Immunoglobulin Idiotypes / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D018655:Lymphocyte Count; D051379:Mice; D008805:Mice, Inbred A; D013234:Stem Cells / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology; D010132:p-Azobenzenearsonate / Q000276:immunology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007130:Immunoglobulin Idiotypes; D007075:Immunoglobulin M; C044203:anti-IgM; D005719:gamma-Globulins; D010132:p-Azobenzenearsonate