Immunohistochemical detection of p53 protein expression in HPV-induced condyloma acuminatum.
Immunohistochemical peroxidase staining for p53 protein was performed on 22 condyloma acuminatum tissue samples from patients infected with human papillomavirus (HPV). The purpose of our study was to understand the benign character of this syndrome. The patients studied were infected by HPV type 6 and 11. Two monoclonal antibodies, PAbs DO-1 and 240, were used to detect the p53 protein. Overexpression of wild-type p53 was found in the nuclei of the basal cell layers. In healthy tissues and non-infected patients no p53 protein expression was detected. We would like to speculate that infection with HPVs and their viral protein E7, which is implicated in disruption of normal growth, may regulate the induction of wild-type p53 over-expression, as is known for DNA-damaging agents such as UV- or X-radiation.
Tendler
Y
Y
Clinical & Research Laboratories, Nahariya Regional Hospital, Israel.
Schwartz
Y
Y
Reshef
R
R
Shasha
S M
SM
Rotter
V
V
Shkolnik
T
T
D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000378:metabolism; D003218:Condylomata Acuminata / Q000378:metabolism* / Q000473:pathology; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005260:Female; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification / Q000378:metabolism; D030361:Papillomavirus Infections / Q000378:metabolism* / Q000473:pathology; D010409:Penile Diseases / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000378:metabolism / Q000473:pathology; D013194:Staining and Labeling; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000235:genetics; D014412:Tumor Virus Infections / Q000378:metabolism* / Q000473:pathology; D014845:Vulvar Diseases / Q000378:metabolism* / Q000473:pathology
D000911:Antibodies, Monoclonal; D004279:DNA, Viral; D016159:Tumor Suppressor Protein p53