title: [Kinetic analysis of interaction of human immunodeficiency virus reverse transcriptase with alkaloids].
abstract: The interactions of HIV-I reverse transcriptase with some alkaloids were studied. Among nine compounds tested three--berberine, palmatine and sanguiritrine--inhibited RT. The dependence of the inhibition on the type of template-primer was also demonstrated. The kinetic analysis as well as circular dichroism experiments suggest the complex mechanism of RT inhibition by alkaloids. This mechanism includes both enzyme-alkaloid and alkaloid-template interactions; the latter effect also results in RT inhibition.
authors.forename: S O
authors.initials: SO
authors.lastname: Gudima
authors.forename: L V
authors.initials: LV
authors.lastname: Memelova
authors.forename: V B
authors.initials: VB
authors.lastname: Borodulin
authors.forename: D K
authors.initials: DK
authors.lastname: Pokholok
authors.forename: B M
authors.initials: BM
authors.lastname: Mednikov
authors.forename: O N
authors.initials: ON
authors.lastname: Tolkachev
authors.forename: S N
authors.initials: SN
authors.lastname: Kochetkov
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D001599:Berberine / Q000494:pharmacology; D001600:Berberine Alkaloids / Q000494:pharmacology; D002942:Circular Dichroism; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology*; D007700:Kinetics; D018894:Reverse Transcriptase Inhibitors
chemical_list: D000470:Alkaloids; D001600:Berberine Alkaloids; D018894:Reverse Transcriptase Inhibitors; D001599:Berberine; C070444:sanguiritrine; D054303:HIV Reverse Transcriptase; C005413:palmatine