An improved method for chemical devitellinization of X-gal stained Drosophila embryos.
In Drosophila developmental biological studies, X-gal staining is commonly employed to study the spatio-temporal expression of the lacZ reporter gene in the transformed flies or their embryos. Study of the lacZ pattern in embryos often suffers from the lack of an efficient and high yielding technique for devitellinization of X-gal stained embryos. Devitellinization techniques employed during antibody staining, in situ hybridization or embryonic cuticular preparations generally do not give satisfactory results when used for similar purpose in X-gal stained embryos. This results in the flaky appearance of the blue stain. We present here an improved chemical devitellinization technique which gives a high yield of devitellinized embryos and a better resolution of the X-gal staining pattern.
Singh
A
A
Drosophila Stock Center, School of Life Science, Indore, India.
Kango
M
M
Sinha
P
P
D000818:Animals; D004330:Drosophila / Q000187:drug effects* / Q000196:embryology; D004625:Embryo, Nonmammalian / Q000187:drug effects; D005697:Galactosides; D007211:Indoles; D013194:Staining and Labeling; D014817:Vitelline Membrane / Q000187:drug effects*
D005697:Galactosides; D007211:Indoles; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside