Specific vesicular acetylcholine transporter promoters lie within the first intron of the rat choline acetyltransferase gene.
The sequence encoding the vesicular acetylcholine transporter (VAChT) has recently been localized within the first intron of the choline acetyltransferase (ChAT) gene in various species. In rat, we previously identified a class of VAChT mRNAs that may originate from the same promoter region as two ChAT mRNAs. Here, we demonstrate by a detailed analysis of the 5'-noncoding region of the VAChT gene, that two specific VAChT promoters lie within the first intron of the ChAT gene. Two VAChT mRNAs are generated from these promoters. These results demonstrate that the promoter regions of these two genes are intermingled, which highlight the unique organization of the ChAT/VAChT gene locus.
Cervini
R
R
Laboratoire de Génétique Moléculaire de la Neurotransmission et des Processus Neurodégénératifs, Bâtiment CERVI, Groupe Hospitalier Pitié-Salpétrière, Paris, France.
Houhou
L
L
Pradat
P F
PF
Béjanin
S
S
Mallet
J
J
Berrard
S
S
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D002795:Choline O-Acetyltransferase / Q000235:genetics*; D006801:Humans; D007438:Introns; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D014158:Transcription, Genetic; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; C493459:SLC18A3 protein, human; C493461:Slc18a3 protein, rat; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine