title: Isolation of human aquaporin 3 gene.
abstract: Human aquaporin 3 (AQP3) gene was isolated, and its structural organization was characterized. The gene appeared to exist as a single copy in the human genome and to comprise six exons distributing over 7 kilobases. The sizes of the exons are 171, 127, 138, 119, 218, and 1035 base pairs, and those of introns are approximately 3530, 300, 350, 330, and 90 base pairs, respectively. The initiation site of transcription was identified to locate 64 base pairs upstream of the first ATG codon by primer extension analysis and ribonuclease protection assay. The 5'-flanking region has a TATA box, two Sp1 sequences, and some consensus sequences including AP2 sites. With luciferase assay, the 5'-flanking region was demonstrated to have a promoter activity, which is up-regulated 4-fold by phorbol ester. These findings about the genomic clone of human AQP3 will contribute to elucidate the molecular mechanism of transcriptional regulation of AQP3.
authors.affiliation: Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Inase
authors.forename: K
authors.initials: K
authors.lastname: Fushimi
authors.forename: K
authors.initials: K
authors.lastname: Ishibashi
authors.forename: S
authors.initials: S
authors.lastname: Uchida
authors.forename: M
authors.initials: M
authors.lastname: Ichioka
authors.forename: S
authors.initials: S
authors.lastname: Sasaki
authors.forename: F
authors.initials: F
authors.lastname: Marumo
mesh_terms: D000595:Amino Acid Sequence; D051400:Aquaporin 3; D020346:Aquaporins; D001483:Base Sequence; D003062:Codon; D018076:DNA, Complementary; D005091:Exons; D006801:Humans; D007473:Ion Channels / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic
chemical_list: C496027:AQP3 protein, human; D020346:Aquaporins; D003062:Codon; D018076:DNA, Complementary; D007473:Ion Channels; D051400:Aquaporin 3