Immunocytochemical study of lampbrush chromosomes of the urodele Pleurodeles waltl: axial granules are recognized by the mitosis-specific monoclonal antibody MPM-2.
The lampbrush chromosomes of the urodele Pleurodeles waltl have been studied using the mitosis-specific monoclonal antibody MPM-2. Immunofluorescence studies revealed that MPM-2 stains structures associated with axial granules, numerous other chromomeres, telomeres and certain chiasmata. These structures showed a negative reaction with the anti-DNA monoclonal antibody AC-30-10. In course of meiotic condensation of the chromosomes, in growing and maturating oocytes, the number of such structures associated with the chromosome axis was found to diminish progressively. These granular structures have been found to be formed by fine fibrils about 5 nm in diameter. Immunogold labeling confirmed the results of immunofluorescence studies. MPM-2 was also found to stain two other types of structures observed in association with the lampbrush chromosome axis in P waltl, viz the sphere organelle (only in later stages of oogenesis) and the structure known as 'M' which is singular to this material.
Pyne
C K
CK
CNRS-URA 1135, Laboratoire de Génétique du Développement, Université P-et-M-Curie, Paris, France.
Loones
M T
MT
Simon
F
F
Zhou
Z J
ZJ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002875:Chromosomes / Q000648:ultrastructure*; D008540:Meiosis; D008854:Microscopy, Electron; D009865:Oocytes / Q000648:ultrastructure*; D010750:Phosphoproteins / Q000378:metabolism; D010999:Pleurodeles / Q000033:anatomy & histology*
D000911:Antibodies, Monoclonal; D010750:Phosphoproteins