title: Latex agglutination for rapid detection of Pseudomonas pseudomallei antigen in urine of patients with melioidosis.
abstract: A latex agglutination test for the detection of Pseudomonas pseudomallei antigen in urine was evaluated for the rapid diagnosis of melioidosis. With unconcentrated urine, antigen was detected in only 18% of patients with melioidosis overall. However, when urine was concentrated 100-fold, antigen was detected in 47% overall and in 67% of patients with septicaemia or disseminated infection, in whom a rapid diagnosis is most important. The specificity of the test was 100%. These results compared favourably with an enzyme immunoassay. This latex agglutination test is a simple, rapid and highly specific method of diagnosing melioidosis, and will be particularly useful in areas with limited laboratory facilities.
authors.affiliation: Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: M D
authors.initials: MD
authors.lastname: Smith
authors.forename: V
authors.initials: V
authors.lastname: Wuthiekanun
authors.forename: A L
authors.initials: AL
authors.lastname: Walsh
authors.forename: N
authors.initials: N
authors.lastname: Teerawattanasook
authors.forename: V
authors.initials: V
authors.lastname: Desakorn
authors.forename: Y
authors.initials: Y
authors.lastname: Suputtamongkol
authors.forename: T L
authors.initials: TL
authors.lastname: Pitt
authors.forename: N J
authors.initials: NJ
authors.lastname: White
mesh_terms: D000942:Antigens, Bacterial / Q000652:urine*; D016957:Burkholderia pseudomallei / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D007841:Latex Fixation Tests; D008554:Melioidosis / Q000276:immunology / Q000652:urine*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000942:Antigens, Bacterial