The primary structure of a basic (pI 9.0) fatty acid-binding protein from liver of Gallus domesticus.
The complete amino acid sequence of a basic (pI 9.0) fatty acid-binding protein purified from liver of Gallus domesticus was determined by automated Edman degradation of tryptic, CNBr/HFBA and Staphylococcus aureus protease peptides. The protein contains 125 amino acid residues which correspond to a molecular mass of 14094. The identification of the blocked N-terminus Ac-Ala required digestion of a SV-8 peptide with the acylamino acid-releasing enzyme prior to sequence analysis. Sequence comparison shows that chicken liver basic-FABP has a significant similarity to other proteins belonging to the superfamily of intracellular lipid molecule binding proteins. Moreover, these sequence data confirm that basic-FABP probably binds its substrate in a slightly different way when compared with other FABPs. Basic-FABP was submitted to the EMBL Data Library with an accession number of P80226.
Ceciliani
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Istituto di Fisiologia Veterinaria e Biochimica, Università di Milano, Italy.
Monaco
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HL
Ronchi
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S
Faotto
L
L
Spadon
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P
D000468:Alkalies; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry*; D002645:Chickens / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins; D008099:Liver / Q000737:chemistry*; D008969:Molecular Sequence Data; D018996:Myelin P2 Protein / Q000737:chemistry*; D009363:Neoplasm Proteins
D000468:Alkalies; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins