title: Solid phase synthesis of 5'-diphosphorylated oligoribonucleotides and their conversion to capped m7Gppp-oligoribonucleotides for use as primers for influenza A virus RNA polymerase in vitro.
abstract: We have synthesized four different 5'-diphosphorylated oligoribonucleotides, varying in length from 11 to 13 nucleotides by a new solid phase method. After deprotection and partial purification the 5'-diphosphorylated oligoribonucleotides could be converted to capped (m7Gppp) oligoribonucleotides using guanylyl transferase. Radiolabelled capped oligoribonucleotides acted as primers for the influenza A virus RNA polymerase in vitro. The solid phase method described here should also allow the addition of 5'-diphosphates to synthetic oligodeoxyribonucleotides and be capable automation.
authors.affiliation: Chemical Pathology Unit, Sir William Dunn School of Pathology, University of Oxford, UK.
authors.forename: G G
authors.initials: GG
authors.lastname: Brownlee
authors.forename: E
authors.initials: E
authors.lastname: Fodor
authors.forename: D C
authors.initials: DC
authors.lastname: Pritlove
authors.forename: K G
authors.initials: KG
authors.lastname: Gould
authors.forename: J J
authors.initials: JJ
authors.lastname: Dalluge
mesh_terms: D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D009980:Influenza A virus / Q000201:enzymology* / Q000235:genetics*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D010766:Phosphorylation; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry; D012315:RNA Caps / Q000138:chemical synthesis* / Q000737:chemistry; D012367:RNA, Viral / Q000235:genetics
chemical_list: D009843:Oligoribonucleotides; D012315:RNA Caps; C034477:RNA primers; D012367:RNA, Viral; D012313:RNA; D012321:DNA-Directed RNA Polymerases