Studies on human porin: XIII. The type-1 VDAC 'porin 31HL' biotinylated at the plasmalemma of trypan blue excluding human B lymphocytes.
In 1989, we demonstrated for the first time the expression of the VDAC 'Porin 31HL' in the plasmalemma of human B lymphocytes, then giving first evidence of a multi-topological expression of VDAC. Meanwhile, data from this and other laboratories support our proposal of a multi-compartment distribution of the channel in mammalian tissues. Here, by biotinylation of plasmalemma-integrated proteins of proven living B lymphocytes, followed by two-dimensional electrophoresis, immuno- and streptavidin affinity blotting, we show that part of the channel molecules can be labelled at the outer membrane of the cells. Thus, by a relevant approach our results invalidate objections concerning putative cross-reactivity of anti-human Type-1 porin antibodies with non-VDAC proteins at the outer cell membrane.
Jakob
C
C
Max-Planck-Institut für experimentelle Medizin, Abt. Immunchemie, Göttingen, Germany.
Götz
H
H
Hellmann
T
T
Hellmann
K P
KP
Reymann
S
S
Flörke
H
H
Thinnes
F P
FP
Hilschmann
N
N
D001402:B-Lymphocytes / Q000737:chemistry*; D001710:Biotin; D002462:Cell Membrane / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007473:Ion Channels / Q000032:analysis*; D008856:Microscopy, Fluorescence; D018272:Porins / Q000032:analysis*; D014407:Tumor Cells, Cultured; D050995:Voltage-Dependent Anion Channel 1
D007473:Ion Channels; D018272:Porins; C494762:VDAC1 protein, human; D001710:Biotin; D050995:Voltage-Dependent Anion Channel 1