High K-ras mutation rates in goblet-cell-type adenocarcinomas of the lungs.
Adenocarcinomas of the lungs show a variable histology. We have subclassified such lesions into five cell types: hobnail, columnar, polygonal, mixed and goblet cell types, and investigated their relationships with K-ras mutations. Codons 12, 13 and 61 of the K-ras gene in 120 surgically resected pulmonary adenocarcinomas were examined by the mutation-allele-specific amplification method. Point mutations were observed in 10% of the adenocarcinomas limited to K-ras codon 12 and the commonest base substitution (nine cases) was a G to T transversion. Of the five types, goblet cell lesions demonstrated the highest mutation index, which at 100% (6/6) was significantly different from that of all other cell types. No relationship between K-ras mutation and cigarette smoking was observed. From these findings, it appears that development of goblet-cell-type adenocarcinomas of the lung may involve different carcinogenic mechanisms from adenocarcinomas of other subtypes.
Tsuchiya
E
E
Department of Pathology, Cancer Institute, Tokyo, Japan.
Furuta
R
R
Wada
N
N
Nakagawa
K
K
Ishikawa
Y
Y
Kawabuchi
B
B
Nakamura
Y
Y
Sugano
H
H
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D001483:Base Sequence; D002454:Cell Differentiation; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D011905:Genes, ras; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D017354:Point Mutation; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics
D017931:DNA Primers; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)