title: Further characterization of the 5'-flanking region of the rat lactase-phlorizin hydrolase gene.
abstract: The lactase-phlorizin hydrolase gene is widely used as a marker of intestinal differentiation. Recent evidence demonstrating that transcription plays a major role in the regulation of this gene suggests that study of the 5'-flanking region will allow an understanding of how the expression of this gene is controlled. However, sequence, restriction, and primer extension analysis of a rat genomic clone has revealed that previously published data are incomplete. In the present study, we used a directed sequencing strategy to carefully analyze this region. Our expanded analysis of the 5'-flanking region of the lactase-phlorizin hydrolase gene should facilitate future studies of its structure and function.
authors.affiliation: Division of Pediatric Gastroenterology and Nutrition, New England Medical Center, Tufts University School of Medicine, Boston, MA.
authors.forename: M
authors.initials: M
authors.lastname: Verhave
authors.forename: S D
authors.initials: SD
authors.lastname: Krasinski
authors.forename: S M
authors.initials: SM
authors.lastname: Maas
authors.forename: F J
authors.initials: FJ
authors.lastname: Smiers
authors.forename: K
authors.initials: K
authors.lastname: Mishra
authors.forename: N G
authors.initials: NG
authors.lastname: Breeuwsma
authors.forename: H Y
authors.initials: HY
authors.lastname: Naim
authors.forename: R J
authors.initials: RJ
authors.lastname: Grand
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005809:Genes, Regulator; D015698:Genomic Library; D007583:Jejunum / Q000201:enzymology*; D010694:Lactase-Phlorizin Hydrolase / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics; D051381:Rats / Q000235:genetics*; D015183:Restriction Mapping; D016385:TATA Box; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D012313:RNA; D010694:Lactase-Phlorizin Hydrolase