Interaction of concanavalin A with a fraction of dermatan sulfate containing a very high anticoagulant activity.
A dermatan sulfate (DS) fraction with high anticoagulant activity was isolated from the precipitate of the interaction with Concanavalin A (Con A). Under the optimum conditions found, the interaction depends absolutely on the presence of Ca++ or Mn++ ions. The pH curve of the activity showed an acute maximum at pH 4.4. The active fraction showed, in average 5.4 folds higher specific activity than the fraction that remained in the supernatant. Native DS was desulfated by acid hydrolysis: a drastic reduction in the interaction with Con A was observed during the first ten min. at 100 degrees C and a parallel decrease in the anticoagulant activity was also detected. Sulfate liberation followed the same pattern and after 30 min. at 100 degrees C no sulfate groups remain bound.
Fernández de Recondo
M E
ME
Departamento de Ciencias Biológicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Monge
J C
JC
Recondo
E F
EF
D000925:Anticoagulants / Q000378:metabolism; D002118:Calcium; D003208:Concanavalin A / Q000378:metabolism*; D003871:Dermatan Sulfate / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008274:Magnesium
D000925:Anticoagulants; D003208:Concanavalin A; D003871:Dermatan Sulfate; D008274:Magnesium; D002118:Calcium