title: Gene targeting for inflammatory cell adhesion molecules.
abstract: Using gene targeting in mouse embryonic stem cells, it is possible to introduce diverse mutations into specific genes. Using these methods, various laboratories have reported mutations for a variety of inflammatory cell adhesion molecules including CD18, alpha 5 integrin, ICAM-1, P-selectin, and L-selectin; preliminary reports of other mutations are also available. Mutations in CD18 and ICAM-1 cause impaired inflammatory and immune responses, mutations in P-selectin and L-selectin cause decreased leukocyte rolling and emigration, and a mutation in alpha 5 integrin causes embryonic lethality. Gene targeting complements other approaches for analyzing the function of inflammatory cell adhesion molecules.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: D C
authors.initials: DC
authors.lastname: Bullard
authors.forename: E T
authors.initials: ET
authors.lastname: Sandberg
authors.forename: K
authors.initials: K
authors.lastname: Scharffetter-Kochanek
authors.forename: A L
authors.initials: AL
authors.lastname: Beaudet
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000235:genetics* / Q000502:physiology; D018390:Gene Targeting; D007249:Inflammation / Q000235:genetics* / Q000276:immunology; D016023:Integrins / Q000235:genetics / Q000502:physiology; D019041:L-Selectin; D009154:Mutation; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000235:genetics / Q000502:physiology
chemical_list: D015815:Cell Adhesion Molecules; D016023:Integrins; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D019041:L-Selectin