Production of term offspring by in vitro fertilization using old mouse spermatozoa.
We successfully produced offspring of old male BDF1 mice using in vitro fertilization. Although 7 old male mice (33 months of age) were infertile and revealed frequent degeneration in the seminiferous epithelia, 4 of them had spermatozoa in the caudae epididymides. The IVF rate of their sperm with eggs from young ICR mice was very high (82.0%). The production rate of offspring was also satisfactory (61.6%). The present study indicates that the IVF method is useful for producing offspring of aged male mice.
Wakayama
T
T
Department of Veterinary Anatomy, Faculty of Agriculture, University of Tokyo, Japan.
Tanemura
K
K
Suto
J
J
Imamura
K
K
Fukuta
K
K
Mori
H
H
Kuramoto
K
K
Kurohmaru
M
M
Hayashi
Y
Y
D000375:Aging / Q000502:physiology*; D000818:Animals; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary*; D005313:Fetal Death; D007248:Infertility, Male; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*; D013737:Testis / Q000254:growth & development / Q000502:physiology