Expression of E-selectin messenger RNA and protein in rheumatoid arthritis.
OBJECTIVE
To examine the de novo synthesis and cellular distribution of the E-selectin adhesion molecule in synovial tissues obtained from patients with rheumatoid arthritis (RA).


METHODS
Immunohistochemistry techniques combined with in situ hybridization were used to examine RA synovium.


RESULTS
There were numerous endothelial cells positive for E-selectin and E-selectin messenger RNA in the RA synovial membranes. Moreover, E-selectin expression appeared to correlate with inflammatory activity.


CONCLUSIONS
The strong vascular expression of E-selectin indicates an activation of endothelial cells in the recruitment of cells associated with the chronic inflammation of RA.
Kriegsmann
J
J
University of Alabama at Birmingham, Department of Medicine, USA.
Keyszer
G M
GM
Geiler
T
T
Lagoo
A S
AS
Lagoo-Deenadayalan
S
S
Gay
R E
RE
Gay
S
S
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics*; D017931:DNA Primers / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D019040:E-Selectin; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics; D013583:Synovial Membrane / Q000737:chemistry / Q000378:metabolism; D015854:Up-Regulation
D015815:Cell Adhesion Molecules; D017931:DNA Primers; D019040:E-Selectin; D012333:RNA, Messenger