title: Induced mutagenesis by bleomycin in the purple sulfur bacterium Thiocapsa roseopersicina.
abstract: Cell death and mutagenesis in bleomycin-treated cells of Thiocapsa roseopersicina (a purple sulfur bacterium) was studied by cultivation in a semisolid medium (agar-shake technique). This technique has also proven useful in assessing the frequency of antibiotic mutations by detecting and counting individual colonies of Thiocapsa roseopersicina. The frequencies of spontaneous mutants resistant to ampicillin, rifampicin, cloramphenicol, tetracycline, kanamycin, streptomycin, and neomycin were also studied: they ranged between 2 x 10(-9) and 9 x 10(-8). Bleomycin (4 micrograms/ml) sharply increased the frequency of ampicillin-resistant mutants, from 10(-8) (spontaneous) to 4 x 10(-4) (induced), in 17 h. An inducible, error-prone mechanism of DNA synthesis seems to be responsible for this enhancement of the mutagenic effect. this is the first report on the sensitivity to several antibiotics, and capacity of lethality and mutagenesis by bleomycin has been studied in a purple sulfur bacterium.
authors.affiliation: Institute for Fundamental Biology, Autonomous University of Barcelona, Bellaterra, Spain.
authors.forename: V
authors.initials: V
authors.lastname: Pavón
authors.forename: I
authors.initials: I
authors.lastname: Esteve
authors.forename: R
authors.initials: R
authors.lastname: Guerrero
authors.forename: A
authors.initials: A
authors.lastname: Villaverde
authors.forename: N
authors.initials: N
authors.lastname: Gaju
mesh_terms: D000668:Ampicillin Resistance / Q000235:genetics; D001761:Bleomycin / Q000494:pharmacology*; D002841:Chromatiaceae / Q000187:drug effects* / Q000235:genetics*; D004249:DNA Damage; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D016296:Mutagenesis
chemical_list: D004269:DNA, Bacterial; D001761:Bleomycin