title: Detection and identification of Campylobacter coli and Campylobacter jejuni by two-step polymerase chain reaction.
abstract: Flagellin gene was used as target sequence to detect and distinguish C. coli and C. jejuni by a "nested PCR" technique. The method shows a high level of sensitivity and specificity. Application of this rapid diagnostic tool could provide further information about epidemiological and pathogenetic implications of each of these two microorganisms.
authors.affiliation: Department of Food Science, University of Udine, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Comi
authors.forename: P
authors.initials: P
authors.lastname: Ferroni
authors.forename: L
authors.initials: L
authors.lastname: Cocolin
authors.forename: C
authors.initials: C
authors.lastname: Cantoni
authors.forename: M
authors.initials: M
authors.lastname: Manzano
mesh_terms: D001483:Base Sequence; D017000:Campylobacter coli / Q000302:isolation & purification*; D016123:Campylobacter jejuni / Q000302:isolation & purification*; D005408:Flagellin / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D005408:Flagellin