Characterization of a pan-lymphocyte monoclonal antibody useful in differentiating leukemic from normal myeloid progenitors and monocytes from macrophages.
A monoclonal antibody (McAb) designated 3A2 that recognizes a 51 kDa epitope having surface density of 37 x 10(8) per MOLT-4 cells is described. This epitope appears to be expressed on (i) lymphocytes at all stages of differentiation; (ii) leukemic myeloid progenitors; (iii) peripheral blood monocytes (MO). The epitope is specifically absent from normal myeloid progenitors and macrophages. The McAb may, therefore, be useful in studying myeloid lineage leukemias and, as a marker for monocyte to macrophage (MO + MAC) differentiation.
Nadkarni
J S
JS
Immuno-Biochem. Laboratory, Tata Memorial Hospital, Parel, Bombay, India.
Chitnis
V S
VS
Panse
G T
GT
Rao
S G
SG
Nadkarni
J J
JJ
D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000097:blood; D000951:Antigens, Neoplasm / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D007124:Immunoenzyme Techniques; D007938:Leukemia / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009000:Monocytes / Q000276:immunology*; D014411:Neoplastic Stem Cells / Q000276:immunology; D011233:Precipitin Tests; D013234:Stem Cells / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D000939:Epitopes