Primary sequence and post-transcriptional modification pattern of an unusual mitochondrial tRNA(Met) from Tetrahymena pyriformis.
In a previous investigation of the rDNA region in Tetrahymena pyriformis mitochondrial DNA, we identified a putative tRNA(Met) gene [Heinonen et al. (1987) J. Biol. Chem. 262, 2879-2887]. On the basis of Northern hybridization analyses, we suggested that this gene is expressed, even though the resulting tRNA would be unusually small and have an atypical dihydrouridine stem-loop domain. We report here the complete nucleotide sequence and post-transcriptional modification pattern of this tRNA(Met), confirming its predicted primary structure and supporting the view that this structurally aberrant species functions in translation in T. pyriformis mitochondria.
Schnare
M N
MN
Canadian Institute for Advanced Research, Department of Biochemistry, Dalhousie University, Halifax.
Greenwood
S J
SJ
Gray
M W
MW
D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D000077278:RNA, Mitochondrial; D016053:RNA, Protozoan / Q000737:chemistry* / Q000378:metabolism; D012358:RNA, Transfer, Met / Q000737:chemistry* / Q000378:metabolism; D013769:Tetrahymena pyriformis / Q000235:genetics* / Q000378:metabolism
D000077278:RNA, Mitochondrial; D016053:RNA, Protozoan; D012358:RNA, Transfer, Met; D012313:RNA