title: [125I]iomazenil binding in the brains of spontaneously epileptic rats: an ex vivo quantitative autoradiographic study.
abstract: Central benzodiazepine receptor binding was studied in spontaneously epileptic rats (SER) and in their parent strain. Kyoto-Wistar (KW) rats, by ex vivo quantitative autoradiography with [125I]iomazenil. Thirteen-week-old SER developed tonic and absence-like seizures following mild stimulation by tapping at 5 min before injection of the radioligand. When compared with age-matched KW rats, a significant 76% elevation of radioactivity was observed in the SER hippocampus, while there was no difference in hippocampal blood flow obtained using [125I]N-isopropyl-p-iodoamphetamine. Since benzodiazepine receptors act to enhance inhibitory GABA transmission, this finding suggests a possible mechanism for seizure-induced enhancement of inhibition.
authors.affiliation: Department of Radiology, Shiga University of Medical Sciences, Otsu, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nagata
authors.forename: H
authors.initials: H
authors.lastname: Saji
authors.forename: S
authors.initials: S
authors.lastname: Nishizawa
authors.forename: Y
authors.initials: Y
authors.lastname: Yonekura
authors.forename: I
authors.initials: I
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Iida
authors.forename: Y
authors.initials: Y
authors.lastname: Magata
authors.forename: J
authors.initials: J
authors.lastname: Konishi
authors.forename: T
authors.initials: T
authors.lastname: Serikawa
authors.forename: A
authors.initials: A
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000378:metabolism*; D002560:Cerebrovascular Circulation; D004827:Epilepsy / Q000378:metabolism*; D005442:Flumazenil / Q000031:analogs & derivatives* / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D008297:Male; D009928:Organ Specificity; D051381:Rats; D011921:Rats, Inbred WKY; D011922:Rats, Mutant Strains; D011963:Receptors, GABA-A / Q000032:analysis / Q000378:metabolism*; D013045:Species Specificity; D013997:Time Factors
chemical_list: D007457:Iodine Radioisotopes; D011963:Receptors, GABA-A; D005442:Flumazenil; C063038:iomazenil