Inhibitory effects of steroidal saponins on 12-O-tetradecanoylphorbol-13-acetate (TPA)-enhanced 32P-incorporation into phospholipids of HeLa cells and proliferation of human malignant tumor cells.
Certain Lilium plants contain (25S)-spirost-5-ene-3 beta,27-diol glycosides embracing 3-hydroxy-3-methylglutaric acid at the C-27 hydroxy position. One of their derivatives, methyl ester of (25R)-27-O-[(S)-3-hydroxy-3-methylglutaryl]-spirost-5-ene-3 beta,27-diol 3-O-(O-alpha-L-rhamnopyranosyl-(1-->2)-O-[beta-D-glucopyranosyl-(1-->4)] - beta-D-glucopyranoside) was found to inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated 32P-incorporation into the phospholipids of human cervical cancer (HeLa) cells and also to inhibit the proliferation of various kinds of human malignant tumor cells, pancreatic cancer (PANC-1), osteosarcoma (OST), human gastric cancer (HGC-27), pheochromocytoma (PC-12) and HeLa cells, in vitro.
Mimaki
Y
Y
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
Sashida
Y
Y
Kuroda
M
M
Nishino
A
A
Satomi
Y
Y
Nishino
H
H
D002240:Carbohydrate Sequence; D002455:Cell Division / Q000187:drug effects; D004347:Drug Interactions; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D012503:Saponins / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D010743:Phospholipids; D010761:Phosphorus Radioisotopes; D012503:Saponins; D010758:Phosphorus; D013755:Tetradecanoylphorbol Acetate