Effect of enzymes on the growth of human and animal rotaviruses.
The growth of group A human, bovine, equine and porcine rotaviruses were enhanced by pretreatment of virus with pancreatin, trypsin, protease, alkaline phosphatase or pepsin and incorporation of these enzymes in maintenance medium. In contrast, alpha-amylase or lipase inhibited the growth of equine and porcine rotaviruses. The other enzymes, adenosine deaminase, lactase, lysozyme, ribonuclease or triose-phosphate isomerase gave little or no change in the growth of all four rotaviruses.
Sato
K
K
Kyushu Branch Laboratory, National Institute of Animal Health, Kagoshima, Japan.
Tokuhisa
S
S
Inaba
Y
Y
D000243:Adenosine Deaminase / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000494:pharmacology; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology; D002417:Cattle; D010450:Endopeptidases / Q000494:pharmacology; D004798:Enzymes / Q000494:pharmacology*; D006736:Horses; D006801:Humans; D043322:Lactase; D008049:Lipase / Q000494:pharmacology; D009113:Muramidase / Q000494:pharmacology; D010194:Pancreatin / Q000494:pharmacology; D010434:Pepsin A / Q000494:pharmacology; D012260:Ribonucleases / Q000494:pharmacology; D012401:Rotavirus / Q000187:drug effects / Q000254:growth & development*; D013045:Species Specificity; D013552:Swine; D014305:Triose-Phosphate Isomerase / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology; D000516:alpha-Amylases / Q000494:pharmacology; D001616:beta-Galactosidase / Q000494:pharmacology
D000998:Antiviral Agents; D004798:Enzymes; D010194:Pancreatin; D012260:Ribonucleases; D008049:Lipase; D000469:Alkaline Phosphatase; D000516:alpha-Amylases; D043322:Lactase; D009113:Muramidase; D001616:beta-Galactosidase; D010450:Endopeptidases; D014357:Trypsin; D010434:Pepsin A; D000243:Adenosine Deaminase; D014305:Triose-Phosphate Isomerase