Molecular species of phospholipids of interleukin-2-dependent murine cytotoxic T lymphocytes.
We reported that phospholipids of interleukin-2 (IL-2)-dependent murine cytotoxic T lymphocytes (CTL) contained high amounts of ether lipids (Biochem. Mol. Biol. Int., 33, 137-146, 1994). In this study, the molecular species of fatty acids at the sn-1 and sn-2 positions were determined. First, phosphatidylcholine mainly consisted of the 1,2-diacyl subclass (78%) in which the major molecular species was 16:0/18:1 (24%). It should be noted that arachidonic acid was abundant in both 1-O-alkyl-2-acyl subclasses in the form of 18:0/20:4 (21%) even though the content of the subclass was low (8%). Second, phosphatidylethanolamine mainly consisted of ether subclasses (93%) and contained a large amount of arachidonic acid in the forms of either 18:0/20:4 (19%) or 16:0/20:4 (10%). Third, phosphatidylinositol, a putative phospholipid involved in transmembrane signal transduction, had a significant amount of arachidonic acid at the sn-2 positions of 1,2-diacyl and 1-O-alkyl-2-acyl subclasses. Fourth, phosphatidylserine, consisting of mostly the 1-O-alkyl-2-acyl subclass (86%), contained arachidonic acid dominantly in the form of 18:0/20:4 (33%). Considering these results, it appears that CTL have the potential to mediate its signal transduction mediated by the bioactive lipids, i.e. arachidonic acid metabolites and the ether lipids.
Nishihira
J
J
Department of Biochemistry, School of Medicine, Hokkaido University, Sapporo, Japan.
Ishibashi
T
T
Sawamura
Y
Y
Hosokawa
M
M
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis; D007376:Interleukin-2 / Q000378:metabolism / Q000494:pharmacology*; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D051379:Mice; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000737:chemistry / Q000378:metabolism; D010716:Phosphatidylinositols / Q000737:chemistry / Q000378:metabolism; D010718:Phosphatidylserines / Q000737:chemistry / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism
D005227:Fatty Acids; D007376:Interleukin-2; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; D010743:Phospholipids; D016718:Arachidonic Acid