Effect of ribonuclease A and deoxyribonuclease I on immunostaining of Ki-67 in fixed-embedded sections.
Immunostaining of the cell cycle-associated Ki-67 antigen was studied on routinely formalin-fixed and paraffin-embedded tissue sections, using the Ki-67-specific monoclonal antibody MIB-1. Immunomorphological analysis of the Ki-67 immunostaining pattern was carried out following tissue pre-treatments including combinations of microwave heating and trypsinization, as well as of ribonuclease and deoxyribonuclease pre-digestion of the sections. The nucleolar Ki-67 immunostaining after slide pre-treatment by microwave heating followed by trypsinization was reduced only by ribonuclease pre-digestion, if this latter was used before heating. Ki-67 immunostaining was not significantly reduced by deoxyribonuclease treatment. We conclude that Ki-67, located in the nucleoli, may be associated there with nucleolar RNA.
Szekeres
G
G
Laboratory of Histopathology, IMMUNOTECH, Marseille, France.
Le Tourneau
A
A
Benfares
J
J
Audouin
J
J
Diebold
J
J
D003850:Deoxyribonuclease I / Q000494:pharmacology*; D018219:Histiocytoma, Benign Fibrous / Q000737:chemistry*; D006652:Histological Techniques; D006801:Humans; D006965:Hyperplasia / Q000473:pathology; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D008198:Lymph Nodes / Q000737:chemistry*; D016393:Lymphoma, B-Cell / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000032:analysis*; D009687:Nuclear Proteins / Q000032:analysis*; D014066:Palatine Tonsil / Q000737:chemistry* / Q000473:pathology; D012259:Ribonuclease, Pancreatic / Q000494:pharmacology*; D013194:Staining and Labeling
D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D003850:Deoxyribonuclease I; D012259:Ribonuclease, Pancreatic