title: Distribution of Big tau in the central nervous system of the adult and developing rat.
abstract: The diversity of neuronal morphology and function is correlated with specific expression of various microtubule associated proteins (MAPs). One of the major neuronal MAPs, tau, has multiple isoforms formed as a result of alternative splicing and phosphorylation that are differentially expressed during development. Big tau is a high molecular weight isoform that contains an additional large exon (4a) and is expressed primarily by neurons in the peripheral nervous system (PNS). We cloned the complete 4a exon in an expression vector, isolated the recombinant protein and produced antibodies specific to Big tau that were used to localize Big tau in the developing spinal cord and in the adult central nervous system (CNS). In developing spinal cord, Big tau is first expressed in the central projections of the dorsal root ganglia neurons and in motor neurons at embryonic day 18 and postnatal day 2, respectively. In the adult rat CNS, almost all neurons that extend processes into the PNS express Big tau, including all cranial nerve motor nuclei and central processes of most sensory ganglia; of these ganglia, only the bipolar neurons of the olfactory, vestibular and spiral ganglia did not express Big tau. Retinal ganglion cells are the only CNS neurons, whose processes remain entirely within the CNS, that express high levels of Big tau. The limited and specific distribution of Big tau is consistent with a role in stabilizing microtubules in axons that are subjected to great shear forces.
authors.affiliation: Department of Anatomy and Neurobiology, Medical College of Pennsylvania, Philadelphia 19129, USA.
authors.forename: L J
authors.initials: LJ
authors.lastname: Boyne
authors.forename: A
authors.initials: A
authors.lastname: Tessler
authors.forename: M
authors.initials: M
authors.lastname: Murray
authors.forename: I
authors.initials: I
authors.lastname: Fischer
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D001923:Brain Chemistry / Q000502:physiology; D002490:Central Nervous System / Q000737:chemistry* / Q000254:growth & development; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019051:Rhizotomy; D012680:Sensitivity and Specificity; D013116:Spinal Cord / Q000737:chemistry; D016875:tau Proteins / Q000032:analysis* / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D016875:tau Proteins