Identification of methylated metabolites of inorganic arsenic by thin-layer chromatography.
TLC on cellulose plates was used to identify methylated products of inorganic arsenic metabolism (monomethylarsonate and dimethylarsinate) in biological samples. Two solvent systems were tested: methanol-ammonium hydroxide (8:2) and isopropanol-acetic acid-water (10:1:2.5). The latter solvent system produced the most satisfactory separation of radiolabelled methylated arsenic compounds in aqueous solution, in rat liver cytosol incubated with carrier-free or 1 microM [73As]arsenite and in urine of mice given carrier-free [73As]arsenate or 5 mg of [73As]arsenate/kg per os. Oxidation of samples by hydrogen peroxide improved the separation and quantitation of monomethylarsonate in both biological matrices.
Stýblo
M
M
University of North Carolina, Chapel Hill, USA.
Delnomdedieu
M
M
Hughes
M F
MF
Thomas
D J
DJ
D000818:Animals; D001149:Arsenates / Q000378:metabolism / Q000652:urine; D001152:Arsenicals / Q000032:analysis*; D018053:Arsenites / Q000378:metabolism / Q000652:urine; D002101:Cacodylic Acid / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods*; D003600:Cytosol / Q000737:chemistry; D005260:Female; D008099:Liver / Q000737:chemistry*; D008745:Methylation; D051379:Mice; D010084:Oxidation-Reduction; D051381:Rats; D011916:Rats, Inbred F344
D001149:Arsenates; D001152:Arsenicals; D018053:Arsenites; D002101:Cacodylic Acid; C020300:monomethylarsonic acid; C015001:arsenite; C025657:arsenic acid