Serum levels of selected liver proteins following partial hepatectomy in the female rat.
Liver regeneration in the rat following partial hepatectomy (PH) is a frequently used model to study regulatory mechanisms in relation to cell growth and differentiation. In the present study, we analysed quantitative changes in the peripheral circulation of a number of important serum proteins following PH and laparotomy. Alpha-fetoprotein synthesis was induced in the PH rats and remained at very low levels in non-operated controls. Pregnancy-associated murine protein-1 levels and fibronectin levels were lower in the circulation of all operated animals compared with the intact controls. The serum levels of alpha-2 macroglobulin were elevated in all operated animals compared with the non-operated controls. Circulating complement factors C3c and C4 were present at significantly lower levels in PH animals than in rats following laparotomy. Small growth stimulating molecules (< 10 kDa) synthesized by different organs during the regenerative process have been described in the literature, but dialysates of the spleen and liver from non-operated rats and PH rats showed no significant impact on serum levels of the proteins in the present study.
Hau
J
J
Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, UK.
Cervinková
Z
Z
O'Brien
D
D
Stodulski
G
G
Simek
J
J
D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D015932:Complement C3c / Q000032:analysis; D003181:Complement C4 / Q000032:analysis; D005260:Female; D005353:Fibronectins / Q000097:blood; D006498:Hepatectomy; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration; D011257:Pregnancy Proteins / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D000509:alpha-Fetoproteins / Q000032:analysis; D000511:alpha-Macroglobulins / Q000032:analysis
D001798:Blood Proteins; D003181:Complement C4; D005353:Fibronectins; D011257:Pregnancy Proteins; D000509:alpha-Fetoproteins; D000511:alpha-Macroglobulins; C033484:pregnancy-associated murine protein 1; D015932:Complement C3c