title: Correlation of histological tumor vascularization and Doppler sonography in patients with malignant melanoma of the choroid.
abstract: BACKGROUND
  In previous studies Doppler sonography was proven to the useful for diagnostics and follow-up of malignant melanomas of the choroid.
  
  
  METHODS
  To evaluate the correlation between Doppler sonographical findings and the histological tumor vascularization, we examined five eyes of five patients with malignant melanoma of the choroid before enucleation with an ATL Ultramark-8 Duplex scanner and compared these results with computerized planimetry of histological tumor vascularization after immunohistochemical staining of vascular endothelium with anti-factor-VIII antibodies.
  
  
  RESULTS
  We found no definite correlation of flow velocities with histological parameters of tumor vascularization. The tendency was for a decrease in maximum flow velocities as histological vascularization increased.
  
  
  CONCLUSIONS
  Due to (1) the usual lack of sensitivity parameters of the used Duplex device for the clinical user and (2) the unpredictable branching pattern within a melanoma resulting in an unknown angle of incidence of the Doppler beam, we conclude that the quantitative results of Doppler sonography require cautious interpretation. Nevertheless, presence or absence of Doppler shifts is a valuable parameter for the follow-up of irradiated tumors.
authors.affiliation: Duke University Eye Center, Durham, NC 27710, USA.
authors.forename: T
authors.initials: T
authors.lastname: Damms
authors.forename: H
authors.initials: H
authors.lastname: Schäfer
authors.forename: R
authors.initials: R
authors.lastname: Guthoff
authors.forename: P
authors.initials: P
authors.lastname: Winkler
authors.forename: R
authors.initials: R
authors.lastname: Winter
mesh_terms: D001783:Blood Flow Velocity; D002830:Choroid Neoplasms / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000473:pathology*; D004730:Endothelium, Vascular / Q000737:chemistry; D015353:Eye Enucleation; D005169:Factor VIII / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D008545:Melanoma / Q000098:blood supply* / Q000000981:diagnostic imaging / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging* / Q000473:pathology*; D018616:Ultrasonography, Doppler, Duplex
chemical_list: D005169:Factor VIII