[Application of Plasmodium falciparum DNA extract from dried blood spot of falciparum malaria patient in polymerase chain reaction].
Polymerase chain reaction (PCR) primered with primers 3,4 and 5,6 was performed using DNA extracted from dried blood spot of a falciparum malaria patient from Mengpeng Township, Yunnan using four different methods, including STE-proteinase K-SDS, methanol-proteinase K-SDS, Chelex-100 and Chelex-100-proteinase K to amplify DNA of apical membrane antigen 1 (AMA-1). A 900 bp DNA band could be seen on the ethidium bromide-stained agarose gel electrophoresis of the PCR products when DNA was extracted using all the above-mentioned methods except STE-proteinase K-SDS method. DNA extracted with Chelax-100 was recommended.
Zhang
L
L
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Zhan
B
B
Wang
J
J
Feng
X
X
D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000302:isolation & purification*; D006801:Humans; D016778:Malaria, Falciparum / Q000235:genetics / Q000469:parasitology*; D008297:Male; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction
D016054:DNA, Protozoan