title: Structures of the N-linked oligosaccharides on human plasma vitronectin.
abstract: The structures of N-linked oligosaccharides present on human plasma vitronectin were elucidated. Oligosaccharides were released from the vitronectin by N-glycosidase F digestion and tagged with 2-aminopyridine; the pyridylamino-oligosaccharides were then fractionated by anion-exchange and reverse-phase HPLC. Ten major pyridylamino-oligosaccharides were isolated. The linkages and locations of sialic acid residues were determined by desialylation with Salmonella sialidase in combination with acid. The asialo forms were then analyzed by two-dimensional sugar mapping, component sugar analysis and 400-MHz 1H-NMR spectroscopy. The major oligosaccharides of human vitronectin were of the diantennary N-acetyllactosamine type, with a lesser amount of the tri- and a small amount of the mono-antennary type, to which 1-3 mol sialic acid residues were linked, mostly through alpha 2-6 linkages, although alpha 2-3 linkages were also present. The possibility that several binding activities of vitronectin can be ascribed to its glycan moiety was discussed, based on the specific features of the N-linked oligosaccharides on human vitronectin revealed here.
authors.affiliation: Department of Chemistry, Ochanomizu University, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ogawa
authors.forename: A
authors.initials: A
authors.lastname: Yoneda
authors.forename: N
authors.initials: N
authors.lastname: Seno
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: I
authors.initials: I
authors.lastname: Ishizuka
authors.forename: S
authors.initials: S
authors.lastname: Hase
authors.forename: I
authors.initials: I
authors.lastname: Matsumoto
mesh_terms: D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000097:blood / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D019096:Vitronectin
chemical_list: D006023:Glycoproteins; D009844:Oligosaccharides; D019096:Vitronectin