title: Bone marrow-derived murine mast cells migrate, but do not degranulate, in response to chemokines.
abstract: We have determined that several chemokines induce mast cell migration in vitro. This directed migration is dependent on the presence of particular extracellular matrix proteins and the activation status of the cells. Mast cell haptotactic responses were observed in response to various chemokines on vitronectin-, laminin-, and fibronectin-coated filters. Unstimulated mast cells were chemoattracted only by monocyte chemotactic protein-1 and RANTES on vitronectin-coated and, to a lesser extent, laminin-coated filters, whereas IgE-activated mast cells migrated in response to monocyte chemotactic protein-1, regulated on activation normal T expressed and secreted, platelet factor-4, and macrophage inflammatory protein-1 alpha on all three matrix proteins. No significant migration was observed on collagen type IV-coated or uncoated filters. Mast cell migration in response to chemokines on extracellular matrices and its enhancement by IgE-dependent activation provide a mechanism by which cells may be drawn to sites of inflammation. Chemokine-induced mast cell recruitment may be particularly relevant in host defense responses to parasitic infections, allergic reactions, Jones-Mote reactions, and in wound healing.
authors.affiliation: Clinical Services Program, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702.
authors.forename: D
authors.initials: D
authors.lastname: Taub
authors.forename: J
authors.initials: J
authors.lastname: Dastych
authors.forename: N
authors.initials: N
authors.lastname: Inamura
authors.forename: J
authors.initials: J
authors.lastname: Upton
authors.forename: D
authors.initials: D
authors.lastname: Kelvin
authors.forename: D
authors.initials: D
authors.lastname: Metcalfe
authors.forename: J
authors.initials: J
authors.lastname: Oppenheim
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D015550:Cell Degranulation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D002630:Chemotactic Factors / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects; D016207:Cytokines / Q000494:pharmacology; D016326:Extracellular Matrix Proteins; D006801:Humans; D008222:Lymphokines / Q000494:pharmacology; D019402:Macrophage Inflammatory Proteins; D008407:Mast Cells / Q000187:drug effects* / Q000502:physiology*; D051379:Mice; D018945:Monocyte Chemoattractant Proteins; D015846:Monokines / Q000494:pharmacology; D010978:Platelet Factor 4 / Q000494:pharmacology
chemical_list: D054407:Chemokine CCL4; D018946:Chemokine CCL5; D002630:Chemotactic Factors; D016207:Cytokines; D016326:Extracellular Matrix Proteins; D008222:Lymphokines; D019402:Macrophage Inflammatory Proteins; D018945:Monocyte Chemoattractant Proteins; D015846:Monokines; D010978:Platelet Factor 4