Rapid and easy procedure for the determination of immunoglobulin class and light chain type of anti-lactate dehydrogenase antibodies in macro-lactate dehydrogenase.
We developed an easy to perform and rapid method for determination of the immunoglobulin class and light chain type of anti-enzyme antibodies present in macro-enzymes. The procedure is a combination of two routinely used laboratory kits, and it allows identification of the antibody involved within 1.5 hour. The applicability of the method was demonstrated for macro-lactate dehydrogenase.
Kleinveld
H A
HA
Department of Clinical Chemistry, University Hospital, Utrecht, The Netherlands.
Voorbij
H A
HA
van Rijn
H J
HJ
D000743:Anemia, Hemolytic / Q000097:blood / Q000201:enzymology; D000818:Animals; D000936:Antigen-Antibody Complex; D001323:Autoantibodies / Q000097:blood*; D002318:Cardiovascular Diseases / Q000097:blood / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000201:enzymology; D007147:Immunoglobulin Light Chains; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011817:Rabbits / Q000276:immunology; D011933:Reagent Kits, Diagnostic
D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D007147:Immunoglobulin Light Chains; D007527:Isoenzymes; D011933:Reagent Kits, Diagnostic; D007770:L-Lactate Dehydrogenase