Oxidative damage in human liver transplantation.
The aim of this study was to evaluate oxygen-dependent hepatic reperfusion injury in humans following orthotopic liver transplantation. To this end, a number of blood indices of impaired tissue redox balance were monitored in 19 adult patients for 3 weeks after liver transplantation. Both red cell malonaldehyde and plasma lipid peroxides increased significantly soon after organ reperfusion. This finding was consistently accompanied by decreased plasma vitamin E and red cell total glutathione. A peak of oxidative stress, as measured by the parameters monitored, was evident within 24 h after reperfusion, together with a maximum expression of cytolysis, as measured by plasma alanine aminotransferase. The occurrence of redox imbalance after hepatic reperfusion was shown to be linearly related to irreversible cell damage. As regards the low plasma levels of the two antioxidants after reperfusion, only that of vitamin E appeared statistically related to oxidative stress. With the background of an increasing body of proof, mainly from animal models, the involvement of toxic oxygen metabolites in hepatic cytolysis following orthotopic liver transplantation appears likely. The statistical correlation among the markers of redox imbalance monitored indicates their combined use in further investigation.
Biasi
F
F
Department of Experimental Medicine and Oncology, University of Torino, Italy.
Bosco
M
M
Chiappino
I
I
Chiarpotto
E
E
Lanfranco
G
G
Ottobrelli
A
A
Massano
G
G
Donadio
P P
PP
Vaj
M
M
Andorno
E
E
D000293:Adolescent; D000328:Adult; D000368:Aged; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000031:analogs & derivatives / Q000097:blood; D019803:Glutathione Disulfide; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood*; D016031:Liver Transplantation / Q000473:pathology / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D008875:Middle Aged; D018384:Oxidative Stress; D015424:Reperfusion; D015427:Reperfusion Injury / Q000097:blood / Q000175:diagnosis*; D014810:Vitamin E / Q000097:blood
D015415:Biomarkers; D008054:Lipid Peroxides; D014810:Vitamin E; D008315:Malondialdehyde; D001219:Aspartate Aminotransferases; D005978:Glutathione; D019803:Glutathione Disulfide