Cytokine regulation of early B-lymphopoiesis assessed in culture.
Lymphohemopoietic progenitors that are capable of expressing B-cell and myeloid lineages may be cultured from bone marrow cells of adult mice by using a two-step methylcellulose culture system. In this system, the primary colonies expressing myeloid lineages are plated in secondary culture for B-lymphoid colony formation. We have observed that combinations of two factors based on steel factor (SLF), such as SLF plus interleukin (IL)-6, SLF plus granulocyte colony-stimulating factor (G-CSF), and SLF plus IL-11 support the differentiation and proliferation of B-cell progenitors from the lymphohemopoietic progenitors. Surprisingly, IL-3 failed to support B lymphopoiesis either alone or in combination with other factors. In addition, when added to permissive culture conditions, IL-3 and IL-1 independently inhibited the B-cell potential of the primary colonies. The inhibitory effects of IL-3 and IL-1 observed in this in vitro system may be significant in the selection of cytokine combinations for in vitro expansion of hemopoietic stem cells.
Hirayama
F
F
Ralph H. Johnson Department of Veterans Affairs Medical Center, Charleston, SC 29401, USA.
Ogawa
M
M
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects*; D001854:Bone Marrow Cells; D016466:CHO Cells; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006224:Cricetinae; D017077:Culture Media, Conditioned; D004347:Drug Interactions; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006410:Hematopoiesis / Q000187:drug effects*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology*; D007378:Interleukins / Q000494:pharmacology; D008747:Methylcellulose; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D019089:Stem Cell Factor
D017077:Culture Media, Conditioned; D016298:Hematopoietic Cell Growth Factors; D007378:Interleukins; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D016179:Granulocyte Colony-Stimulating Factor; D008747:Methylcellulose