title: Studies on human porin: XIII. The type-1 VDAC 'porin 31HL' biotinylated at the plasmalemma of trypan blue excluding human B lymphocytes.
abstract: In 1989, we demonstrated for the first time the expression of the VDAC 'Porin 31HL' in the plasmalemma of human B lymphocytes, then giving first evidence of a multi-topological expression of VDAC. Meanwhile, data from this and other laboratories support our proposal of a multi-compartment distribution of the channel in mammalian tissues. Here, by biotinylation of plasmalemma-integrated proteins of proven living B lymphocytes, followed by two-dimensional electrophoresis, immuno- and streptavidin affinity blotting, we show that part of the channel molecules can be labelled at the outer membrane of the cells. Thus, by a relevant approach our results invalidate objections concerning putative cross-reactivity of anti-human Type-1 porin antibodies with non-VDAC proteins at the outer cell membrane.
authors.affiliation: Max-Planck-Institut für experimentelle Medizin, Abt. Immunchemie, Göttingen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Jakob
authors.forename: H
authors.initials: H
authors.lastname: Götz
authors.forename: T
authors.initials: T
authors.lastname: Hellmann
authors.forename: K P
authors.initials: KP
authors.lastname: Hellmann
authors.forename: S
authors.initials: S
authors.lastname: Reymann
authors.forename: H
authors.initials: H
authors.lastname: Flörke
authors.forename: F P
authors.initials: FP
authors.lastname: Thinnes
authors.forename: N
authors.initials: N
authors.lastname: Hilschmann
mesh_terms: D001402:B-Lymphocytes / Q000737:chemistry*; D001710:Biotin; D002462:Cell Membrane / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007473:Ion Channels / Q000032:analysis*; D008856:Microscopy, Fluorescence; D018272:Porins / Q000032:analysis*; D014407:Tumor Cells, Cultured; D050995:Voltage-Dependent Anion Channel 1
chemical_list: D007473:Ion Channels; D018272:Porins; C494762:VDAC1 protein, human; D001710:Biotin; D050995:Voltage-Dependent Anion Channel 1