Multifunctional activity of the extracellular domain of the M-type (180 kDa) membrane receptor for secretory phospholipases A2.
M-type (180 kDa) receptors for secretory phospholipases A2 (sPLA2s) are thought to mediate some of the physiological effects of group I sPLA2, including smooth muscle contraction and cell proliferation. The M-type sPLA2 receptor is a large glycoprotein composed of several distinct extracellular domains which belongs to the C-type lectin superfamily. This receptor binds with high affinity both pancreatic group I and inflammatory group II sPLA2s as well as various sPLA2s purified from snake venoms. This paper shows that the rabbit M-type sPLA2 receptor is a multifunctional protein which is able to promote cell adhesion on type I and IV collagens most probably via its N-terminal fibronectin-like type II domain. It also shows that binding of sPLA2s to a recombinant soluble form of this receptor is associated with a noncompetitive inhibition of phospholipase A2 activity.
Ancian
P
P
Institute de Pharmacologie Moléculaire et Cellulaire, Valbonne, France.
Lambeau
G
G
Lazdunski
M
M
D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D003094:Collagen / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry; D005110:Extracellular Space; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009838:Oligodeoxyribonucleotides / Q000737:chemistry; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D054467:Phospholipases A2; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000737:chemistry*; D054507:Receptors, Phospholipase A2; D011994:Recombinant Proteins; D012995:Solubility; D013329:Structure-Activity Relationship
D015815:Cell Adhesion Molecules; D004791:Enzyme Inhibitors; D009838:Oligodeoxyribonucleotides; D011956:Receptors, Cell Surface; D054507:Receptors, Phospholipase A2; D011994:Recombinant Proteins; D003094:Collagen; D010741:Phospholipases A; D054467:Phospholipases A2