The role of cAMP- and Ca(2+)-dependent intracellular mechanisms in the control of oxytocin and vasopressin secretion by bovine ovarian granulosa cells in vitro.
The secretion of oxytocin and arginine-vasopressin was demonstrated in bovine granulosa cell culture. It was found that dbcAMP or 3-isobutyl-1-methyl-xanthine (an inhibitor of intracellular cAMP metabolization) additions increased both oxytocin and vasopressin release. The Ca2+ ionophore A23187 also stimulated, while the Ca2+ channel blocker verapamil inhibited the secretion of both nonapeptide hormones. These results suggest the involvement of cAMP- and Ca(2+)-dependent intracellular mechanisms in the stimulation of both oxytocin and vasopressin secretion by bovine granulosa cells.
Sirotkin
A V
AV
Research Institute of Animal Production, Nitra, Slovakia.
Nitray
J
J
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D000242:Cyclic AMP / Q000378:metabolism*; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D010121:Oxytocin / Q000378:metabolism*; D015398:Signal Transduction; D014700:Verapamil / Q000494:pharmacology
D001127:Arginine Vasopressin; D000001:Calcimycin; D010121:Oxytocin; D003994:Bucladesine; D014700:Verapamil; D000242:Cyclic AMP; D002118:Calcium; D015056:1-Methyl-3-isobutylxanthine