Characterization and comparison of the polyclonal immune response to purified PLP and DM-20 in the Lewis rat: determination of a new antigenic determinant.
Lewis rat polyclonal antibodies raised against pure PLP and DM-20 were characterized by indirect and competitive ELISA assays. Anti-PLP antibodies showed a good cross-reactivity with DM-20 and vice versa, suggesting that the chief B-cell epitopes are the same on both proteolipids. Three antigenic determinants, PLP 57-70, PLP 200-218 and the carboxyl-terminal sequence PLP 262-276, were identified for both proteolipids. Peptide PLP 57-70 represents a new B-cell epitope. The major hydrophilic loop (PLP 116-150), deleted in DM-20, did not show any antigenic activity. The study of polyclonal immune response to PLP and DM-20 will be helpful in resolving their potential role in the development and course of experimental allergic encephalomyelitis (EAE) and multiple sclerosis (MS).
Klinguer
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C
Laboratoire de Chimie Organique des Substances Naturelles, CNRS URA 31, Université Louis Pasteur, Strasbourg, France.
Trifilieff
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E
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D018604:Epitope Mapping; D000939:Epitopes / Q000032:analysis*; D007106:Immune Sera / Q000032:analysis; D007118:Immunoassay; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; D011510:Proteolipids / Q000737:chemistry / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew
D000939:Epitopes; D007106:Immune Sera; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; C489071:Plp1 protein, rat; D011510:Proteolipids