title: Post-translational modifications of apolipoprotein A-I and Po proteins in the avian peripheral nerve.
abstract: Apolipoprotein A-I (apo A-I), a soluble lipid transporter, and Po, the major glycoprotein of myelin, are actively synthesized during myelination. To explore the status of post-translational modifications of these proteins in the avian PNS during rapid myelination, endoneurial slices from one day old chick sciatic nerves were incubated with various radioactive precursors that could serve as indicators of such processes. The proteins were isolated from the incubation medium (secreted fraction), the 1% Triton-X-100-soluble intracellular-endoneurial (intracellular) fraction, and myelin-related and purified compact myelin fractions by immunoprecipitation with monospecific anti-apo A-I and or anti-Po antisera. Our results demonstrated that secreted apo A-I is fatty acylated, but not phosphorylated or sulfated. Avian Po protein was phosphorylated by a phorbol ester sensitive protein kinase. Sulfation, as well as fatty acylation, of avian Po protein was observed in organ culture using highly sensitive methods of detection. These results indicate that fatty acylation of secreted apo A-I and phosphorylation, sulfation and fatty acylation of Po have been conserved during evolution, and that these post-translational modifications may play a common function in various species.
authors.affiliation: Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Lemieux
authors.forename: S
authors.initials: S
authors.lastname: Giannoulis
authors.forename: W C
authors.initials: WC
authors.lastname: Breckenridge
authors.forename: C
authors.initials: C
authors.lastname: Mezei
mesh_terms: D000818:Animals; D016632:Apolipoprotein A-I / Q000378:metabolism*; D002645:Chickens; D018993:Myelin P0 Protein; D009185:Myelin Proteins / Q000378:metabolism*; D009924:Organ Culture Techniques; D011499:Protein Processing, Post-Translational; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000378:metabolism*
chemical_list: D016632:Apolipoprotein A-I; D018993:Myelin P0 Protein; D009185:Myelin Proteins