Alteration of liver glutathione S-transferase and protease activities by cobalt chloride treatment of rats.
Effects of cobalt chloride on liver glutathione S-transferase and protease activities were studied. When cobalt chloride (60 mg/kg) was given to rats, liver microsomal glutathione S-transferase and protease activities were significantly increased 24 hr after the injection, whereas glutathione peroxidase activity in microsomes was decreased. The increase in glutathione S-transferase by N-ethylmaleimide was similar to that of the control, indicating that the increase in the transferase activity by cobalt chloride is not due to a modification of the sulfhydryl group of the enzyme. Immunochemical analysis of the liver microsomes did not detect any proteolytic product of microsomal glutathione S-transferase. In puromycin- or actinomycin D-treated rats, an increase in the transferase activity caused by cobalt chloride treatment was depressed. Thus it was suggested that liver microsomal glutathione S-transferase is induced by cobalt chloride treatment, but not activated by limited proteolysis via microsomal protease.
Daido
A
A
Laboratory of Physiology and Pharmacology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
Aniya
Y
Y
D000818:Animals; D003035:Cobalt / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology; D004586:Electrophoresis; D004790:Enzyme Induction / Q000187:drug effects; D005982:Glutathione Transferase / Q000378:metabolism* / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000494:pharmacology; D015151:Immunoblotting; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D011691:Puromycin / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
D003609:Dactinomycin; D003035:Cobalt; D011691:Puromycin; D006861:Hydrogen Peroxide; D005982:Glutathione Transferase