title: Peptide binding and presentation by mouse CD1.
abstract: CD1 molecules are distantly related to the major histocompatibility complex (MHC) class I proteins. They are of unknown function. Screening random peptide phage display libraries with soluble empty mouse CD1 (mCD1) identified a peptide binding motif. It consists of three anchor positions occupied by aromatic or bulky hydrophobic amino acids. Equilibrium binding studies demonstrated that mCD1 binds peptides containing the appropriate motif with relatively high affinity. However, in contrast to classical MHC class I molecules, strong binding to mCD1 required relatively long peptides. Peptide-specific, mCD1-restricted T cell responses can be raised, which suggests that the findings are of immunological significance.
authors.affiliation: Department of Immunology, La Jolla, CA 92037, USA.
authors.forename: A R
authors.initials: AR
authors.lastname: Castaño
authors.forename: S
authors.initials: S
authors.lastname: Tangri
authors.forename: J E
authors.initials: JE
authors.lastname: Miller
authors.forename: H R
authors.initials: HR
authors.lastname: Holcombe
authors.forename: M R
authors.initials: MR
authors.lastname: Jackson
authors.forename: W D
authors.initials: WD
authors.lastname: Huse
authors.forename: M
authors.initials: M
authors.lastname: Kronenberg
authors.forename: P A
authors.initials: PA
authors.lastname: Peterson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D015703:Antigens, CD / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D018949:Antigens, CD1; D002460:Cell Line; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection
chemical_list: D015703:Antigens, CD; D018949:Antigens, CD1; D010455:Peptides