Molecular identification of an IgE-dependent histamine-releasing factor.
An immunoglobulin E (IgE)-dependent histamine-releasing factor (HRF) produced by lymphocytes of atopic children and present in biological fluids of allergic patients has been identified and purified. Amino-terminal sequencing revealed extensive homology to a mouse protein, p21, and its human homolog, p23. Both recombinant proteins caused histamine release from the human basophils of a subpopulation of donors, and this release was dependent on IgE. Polyclonal antibodies recognized and removed the biological activity of recombinant and native HRF. HRF identifies a heterogeneity of IgE and is believed to play a prominent role in chronic allergic disease processes.
MacDonald
S M
SM
Department of Medicine, Johns Hopkins University School of Medicine, Johns Hopkins Asthma and Allergy Center, Baltimore, MD 21224, USA.
Rafnar
T
T
Langdon
J
J
Lichtenstein
L M
LM
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D001491:Basophils / Q000276:immunology; D014408:Biomarkers, Tumor; D002460:Cell Line; D003001:Cloning, Molecular; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D007700:Kinetics; D008222:Lymphokines / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D000090324:Tumor Protein, Translationally-Controlled 1
D000906:Antibodies; D014408:Biomarkers, Tumor; D008222:Lymphokines; D011993:Recombinant Fusion Proteins; D000090324:Tumor Protein, Translationally-Controlled 1; D007073:Immunoglobulin E