title: Expression of gonadotrophin subunit genes in sheep that were homozygous carriers and non-carriers of the Booroola fecundity gene FecB.
abstract: Homozygous carriers (BB) of the Booroola fecundity gene FecB are characterized by high plasma concentrations of immunoreactive or biologically active FSH and, to a lesser extent, of immunoreactive LH, relative to non-carriers (++). Bovine cDNA probes for the alpha gonadotrophin, FSH beta and LH beta genes were used to investigate FecB-specific differences in the mRNA species for the gonadotrophin subunits in pituitaries obtained from ++ and BB mid-luteal phase ovary-intact ewes, ovariectomized ewes and ovary-intact or ovariectomized ewes with hypothalamic-pituitary disconnection (HPD) given the same regimen of pulsatile GnRH. No FecB-specific differences in the number or size of mRNA transcripts detected by northern blotting were noted for any of these genes. Densitometry of the northern blots revealed no significant FecB-specific differences in the relative amounts of mRNA encoding alpha gonadotrophin, FSH beta or LH beta in the pituitaries from any of the experimental groups of ++ and BB sheep. Furthermore, there were no significant FecB-specific differences in the pituitary content of FSH or LH in these animals, despite significantly higher plasma concentrations of FSH in the ovary-intact and ovariectomized HPD groups. These data show that whereas the FecB gene causes increased plasma concentrations of FSH, no consistent effects can be demonstrated on pituitary gonadotrophin content or on gonadotrophin subunit gene transcription, using northern analysis.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
authors.forename: J S
authors.initials: JS
authors.lastname: Fleming
authors.forename: P J
authors.initials: PJ
authors.lastname: Greenwood
authors.forename: D A
authors.initials: DA
authors.lastname: Heath
authors.forename: N L
authors.initials: NL
authors.lastname: Hudson
authors.forename: S
authors.initials: S
authors.lastname: Lun
authors.forename: L
authors.initials: L
authors.lastname: Shaw
authors.forename: K P
authors.initials: KP
authors.lastname: McNatty
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D005260:Female; D005298:Fertility / Q000235:genetics*; D005640:Follicle Stimulating Hormone / Q000235:genetics; D015870:Gene Expression; D015292:Glycoprotein Hormones, alpha Subunit / Q000235:genetics*; D006720:Homozygote; D007986:Luteinizing Hormone / Q000235:genetics; D010052:Ovariectomy; D012756:Sheep / Q000235:genetics*
chemical_list: D015292:Glycoprotein Hormones, alpha Subunit; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone