[Detection of mycobacterial DNA from paraffin-embedded tissue in lung and lymphoid epithelial granulomas].
Standard techniques for the detection of mycobacteria in granulomatous diseases can be inadequate. We analysed 71 formalin fixed and paraffin-embedded tissue blocks from 68 non-immunocompromised patients with caseating, non-caseating, scarred, and miliary granulomas of lung and lymph nodes. A reamplification PCR protocol was established to detect a 123 bp product of the repetitive insertion sequence IS986/IS6110. After exclusion of 6 PCR-negative cases with clinical sarcoidosis 97% of lung tissue blocks with more than 10% caseating and non-caseating granulomas contained mycobacterial DNA. By routine microbiology mycobacteria could be detected in 78% of the patients. Scarred granulomas were PCR-negative. All miliary granulomas were PCR-positive. Lymph nodes showed comparable results. We think that this method facilitates aetiologic analysis of granulomatous diseases especially when the suspicous tissue is fixed and microbiology is not available.
Bohle
R M
RM
Institut für Pathologie, Universität Giessen.
Heidemann
A
A
Borkhardt
A
A
Velcovsky
H G
HG
Altmannsberger
H M
HM
D004269:DNA, Bacterial / Q000032:analysis*; D004848:Epithelium / Q000382:microbiology / Q000473:pathology; D006099:Granuloma / Q000382:microbiology* / Q000473:pathology*; D006652:Histological Techniques; D006801:Humans; D008171:Lung Diseases / Q000382:microbiology / Q000473:pathology*; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology*; D008206:Lymphatic Diseases / Q000382:microbiology / Q000473:pathology*; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000473:pathology*; D010232:Paraffin; D016133:Polymerase Chain Reaction / Q000379:methods*
D004269:DNA, Bacterial; D010232:Paraffin