A marrow harvest procedure under local anesthesia.
This report details a bone marrow harvest procedure performed outside the hospital setting under local anesthesia, thereby avoiding many of the risks associated with the traditional surgical procedure. In approximately 30 minutes, 450 milliliters of marrow can be collected from eight bone punctures, containing a median of 4.18 x 10(9) cells and 33 x 10(6) progenitor cells as defined by CD34 expression. Reinfusion of a median 1.2 x 10(6) CD34+ cells/kg in 10 breast cancer and lung cancer patients after dose-intensive chemotherapy resulted in the recovery of granulocytes > 100/mm3 by day 14 and platelets > 20,000 by day 21. Without progenitor cell support, such recoveries could take 30 and 40 days, respectively. Collection of marrow using this protocol does not compromise the engraftment capability of the progenitor cells, seldom necessitates blood product support, is safer for the patient, and reduces the cost of harvesting by 75% compared to inpatient or day surgery procedures.
Dicke
K A
KA
Arlington Cancer Center, TX 76012, USA.
Hood
D L
DL
Hanks
S
S
Vaughan
M
M
Fulbright
L
L
Dicke
J A
JA
Arneson
M
M
Blumenschein
G
G
D000772:Anesthesia, Local; D000970:Antineoplastic Agents / Q000008:administration & dosage; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001853:Bone Marrow / Q000473:pathology*; D001943:Breast Neoplasms / Q000473:pathology / Q000628:therapy*; D002330:Carmustine / Q000008:administration & dosage; D003131:Combined Modality Therapy; D005047:Etoposide / Q000008:administration & dosage; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology / Q000628:therapy*; D008942:Mitoxantrone / Q000008:administration & dosage; D010045:Outpatients; D010147:Pain Measurement; D013852:Thiotepa / Q000008:administration & dosage; D014021:Tissue Preservation / Q000379:methods; D014182:Transplantation, Autologous
D000970:Antineoplastic Agents; D005047:Etoposide; D013852:Thiotepa; D008942:Mitoxantrone; D002330:Carmustine