The use of surface immunofluorescence assay (SIFA) in the microbiological diagnosis of Lyme borreliosis: a case report.
In this report we describe the use of a newly developed immunofluorescence technique performed with living spirochetes to detect serum antibody to B. burgdorferi s.l. in a case of early Lyme borreliosis. The immunofluorescence method used (surface immunofluorescence assay: SIFA) proved useful in the serological evaluation of suspected cases of Lyme disease.
Sambri
V
V
Institute of Microbiology, University of Bologna, St. Orsola Hospital, Italy.
Armati
S
S
D'Apote
L
L
Farencena
A
A
Marangoni
A
A
Massaria
F
F
Moroni
A
A
Cevenini
R
R
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D001733:Bites and Stings; D015153:Blotting, Western; D015748:Borrelia burgdorferi Group / Q000276:immunology*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008193:Lyme Disease / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D013987:Ticks / Q000235:genetics / Q000382:microbiology; D013997:Time Factors
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M