title: In vivo analysis of straight-chain and branched-chain fatty acid biosynthesis in three actinomycetes.
abstract: The starter units for branched-chain and straight-chain fatty acid biosynthesis was investigated in vivo in three actinomycetes using stable isotopes. Branched-chain fatty acids, which constitute the majority of the fatty acid pool, were confirmed to be biosynthesized using the amino acid degradation products methylbutyrl-CoA and isobutyrl-CoA as starter units. Straight-chain fatty acids were shown to be constructed using butyrl-CoA as a starter unit. Isomerization of the valine catabolite isobutyryl-CoA was shown to be only a minor source of this butyryl-CoA.
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland at Baltimore 21201, USA.
authors.forename: K K
authors.initials: KK
authors.lastname: Wallace
authors.forename: B
authors.initials: B
authors.lastname: Zhao
authors.forename: H A
authors.initials: HA
authors.lastname: McArthur
authors.forename: K A
authors.initials: KA
authors.lastname: Reynolds
mesh_terms: D000214:Acyl Coenzyme A / Q000378:metabolism; D005227:Fatty Acids / Q000096:biosynthesis*; D007536:Isomerism; D016930:Saccharopolyspora / Q000378:metabolism*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000214:Acyl Coenzyme A; D005227:Fatty Acids; C050106:isobutyryl-coenzyme A; C024343:butyryl-coenzyme A