Isolation and characterization of a rat testis glycosphingolipid based on gangliotetraosylceramide and having a blood group B determinant extended with beta 3-linked N-acetylgalactosamine.
Monoclonal antibody 14.2, directed against the GalNAc beta 3Gal alpha 3Gal beta epitope, was used to monitor the isolation of a novel glycosphingolipid from rat testes. Structural analysis was performed by mass spectrometry, proton NMR spectroscopy, gas chromatography-mass spectrometry after degradation, and exoglycosidase digestion. It was concluded that the structure was [formula: see text] This is the first report on an extended blood group B determinant.
Teneberg
S
S
Department of Medical Biochemistry, Göteborg University, Sweden.
Jovall
P A
PA
Karlsson
H
H
Sjögren
H O
HO
Brodin
T
T
D000017:ABO Blood-Group System / Q000276:immunology*; D000116:Acetylgalactosamine / Q000737:chemistry*; D000818:Animals; D000937:Antigen-Antibody Reactions; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D000939:Epitopes; D005732:Gangliosides; D006028:Glycosphingolipids / Q000032:analysis* / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D051381:Rats; D011920:Rats, Inbred WF; D013737:Testis / Q000737:chemistry*
D000017:ABO Blood-Group System; D000939:Epitopes; C011258:G(A1) ganglioside; D005732:Gangliosides; D006028:Glycosphingolipids; D000116:Acetylgalactosamine