Antigenic specificity of anti-ROS DNA antibodies: involvement of lysyl residues in antigen binding.
The antigenicity of native DNA modified with reactive oxygen species was examined. Goats were immunized with the modified polymer and the antibody response was estimated by direct binding and competition ELISA. The induced antibodies bound ROS-DNA and showed considerable binding to native DNA as well. Specificity analysis of the purified antibodies revealed the recognition of native B-, A- and allied conformations presented by various synthetic polynucleotides. The contribution of lysine residues to the immunochemical binding of purified IgG was investigated by modifying the free amino groups of lysine residues. The modification of lysine residues paralleled loss in IgG binding to ROS-DNA to the extent of 50%, suggesting that such residues might be involved in the antigen binding site of immunoglobulin molecule.
Ara
J
J
Department of Biochemistry, Faculty of Medicine, J. N. Medical College, A.M.U., Aligarh, India.
Ali
R
R
D000215:Acylation; D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002417:Cattle; D004247:DNA / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes / Q000276:immunology*; D006041:Goats; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D008239:Lysine / Q000737:chemistry*; D011119:Polynucleotides / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D017382:Reactive Oxygen Species / Q000737:chemistry
D000974:Antibodies, Antinuclear; D000939:Epitopes; D007074:Immunoglobulin G; D011119:Polynucleotides; D017382:Reactive Oxygen Species; D004247:DNA; D008239:Lysine