title: Human skeletal muscle protein: effect of malnutrition, elective surgery and total parenteral nutrition.
abstract: 1. The concentration of alkali-soluble protein, DNA and RNA in percutaneous muscle biopsy specimens was analysed. Tissue alkali-soluble protein/DNA ratio is a measure of muscle protein concentration, while tissue RNA/DNA ratio may reflect the capacity for protein synthesis. 2. Patients with weight loss due to cancer (n = 6) were compared with metabolically healthy patients before elective surgery (n = 7). Alkali-soluble protein/DNA and RNA/DNA ratios in the weight loss group were 248 (14) g/g and 1.3 (0.1) g/g respectively as compared with 404 (13) g/g and 2.1 (0.1) g/g in otherwise healthy patients. All of the alkali-soluble protein/DNA ratios and 5/6 of the RNA/DNA ratios in the weight loss group were below the 95% confidence interval for the healthy control subjects. 3. Patients undergoing elective open cholecystectomy (n = 7) were studied preoperatively and on days 3, 10, 20 and 30 post-operatively. The alkali-soluble protein/DNA ratio remained unchanged on post-operative day 3 but decreased by 8.7% (P < 0.01), 9.6% (P < 0.05) and 20.4% (P < 0.01) on days 10, 20 and 30 respectively in patients eating at will after the operation. No significant post-operative changes in alkali-soluble protein/DNA ratio were seen in patients given post-operative total parenteral nutrition with (n = 9) or without (n = 7) glycyl-glutamine supplementation for 3 days after surgery. 4. In conclusion, patients with weight loss due to malignant disease have a low muscle protein concentration. Elective surgery of medium magnitude results in a decrease in muscle protein lasting for more than 30 days.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Anesthesiology and Intensive Care, Karolinska Institute, St Görans Hospital, Stockholm, Sweden.
authors.forename: B
authors.initials: B
authors.lastname: Petersson
authors.forename: E
authors.initials: E
authors.lastname: Hultman
authors.forename: K
authors.initials: K
authors.lastname: Andersson
authors.forename: J
authors.initials: J
authors.lastname: Wernerman
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002763:Cholecystectomy; D004247:DNA / Q000032:analysis; D017558:Elective Surgical Procedures; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D009369:Neoplasms / Q000150:complications; D009748:Nutrition Disorders / Q000209:etiology / Q000378:metabolism*; D010289:Parenteral Nutrition, Total; D011184:Postoperative Period; D012313:RNA / Q000032:analysis
chemical_list: D009124:Muscle Proteins; D012313:RNA; D004247:DNA