Frequency of interferon-alpha-secreting blood leukocytes in irradiated and bone-marrow-grafted pigs.
The effects of irradiation were studied on porcine interferon-alpha (IFN-alpha) secreting cells (IFN-alpha SC). IFN-alpha SC were characterized by an ELISPOT assay on non-adherent PBMC following incubation with the transmissible gastroenteritis coronavirus. In vitro irradiation of PBMC was followed by a decrease in the number of IFN-alpha SC while IFN-gamma production and cell viability were not affected. These data indicate that porcine IFN-alpha SC are relatively radiosensitive. Indeed, the frequency of blood IFN-alpha SC decreased markedly and rapidly after in vivo whole body or partial lymphoid irradiation. In addition, within several days of compatible bone-marrow engraftment in the irradiated animals, the number of blood IFN-alpha SC returned to normal values. These data demonstrate that circulating porcine IFN-alpha SC are derived from bone-marrow progenitors.
Charley
B
B
INRA, Virologie et immunologie moléculaires, Jouy-en-Josas, France.
Nowacki
W
W
Vaiman
M
M
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism / Q000528:radiation effects; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D016898:Interferon-alpha / Q000378:metabolism*; D007958:Leukocyte Count; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism* / Q000528:radiation effects; D013552:Swine / Q000276:immunology*; D005760:Transmissible gastroenteritis virus / Q000276:immunology
D016898:Interferon-alpha