Peritoneal fluid and HCG influence on steroid production from ovarian tumor tissue, in vitro.
Steroids are produced by malignant and benign epithelial ovarian tumor tissue in vitro, but the regulation is unknown. The effect of peritoneal fluid and beta-HCG on steroid production was analysed. Tissue from 17 malignant or benign epithelial ovarian tumors and 6 normal postmenopausal ovaries were incubated. In 11 cases tissue was incubated with and without addition of the patient's own peritoneal fluid (Part I). Tissue from 22 ovaries was incubated with and without HCG (100 IU/ml medium), (Part II). Furthermore, the release from tumorous and control ovaries of beta-HCG, progesterone, androstenedione and estradiol measured using radioimmunoassay (Part III). Peritoneal fluid stimulated the release of progesterone from both malignant and benign tissue and androstenedione or estradiol stimulated release from benign tissue (Part I). There was no stimulatory effect of HCG on steroid release from malignant tissue. HCG stimulated release of estradiol in benign tissue (Part II). All malignant tumors and 4 out of 6 benign tumors released beta-HCG (Part III). There seems to exist factors in peritoneal fluid which are able to stimulate steroid production from ovarian tumors.
Ridderheim
M
M
Department of Gynecological Oncology, University of Umeå, Sweden.
Mählck
C G
CG
Selstam
G
G
Stendahl
U
U
Bäckström
T
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000735:Androstenedione / Q000096:biosynthesis; D001202:Ascitic Fluid / Q000503:physiopathology*; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D004958:Estradiol / Q000096:biosynthesis; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D010446:Peptide Fragments / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology*; D011374:Progesterone / Q000096:biosynthesis; D013256:Steroids / Q000096:biosynthesis*
D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D013256:Steroids; D000735:Androstenedione; D011374:Progesterone; D004958:Estradiol