Immunohistochemical staining for endotoxin using horseshoe crab factor C in fecal peritonitis.
The object of the present study was to apply a new immunohistochemical staining method to the in vivo determination of endotoxin localization. The immunohistochemical staining method requires factor C (an initiation factor in the Limulus clotting system which is mediated by endotoxin) as a specific ligand of endotoxin, and a newly developed murine monoclonal antibody to factor C. The blood endotoxin level and endotoxin localization in the rat were determined before and at 6, 12 and 24 h after intraperitoneal injection of 0.25 g/kg of fresh rat feces. The greatest blood endotoxin level was achieved at 12 h after the injection, and uptake of endotoxin was evident in Kupffer cells in the liver at 24 h after the injection. There has been no report on determining endotoxin localization in cases of endotoxemia attributed to fecal peritonitis. This new immunohistochemical staining method for determining endotoxin localization will contribute to the histopathological diagnosis of endotoxemia in humans.
Nakao
A
A
Department of Surgery II, Nagoya University School of Medicine, Japan.
Yasui
M
M
Shen
S
S
Takagi
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D060829:Arthropod Proteins; D004731:Endotoxins / Q000097:blood / Q000378:metabolism*; D004792:Enzyme Precursors; D005243:Feces; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D010538:Peritonitis / Q000209:etiology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012697:Serine Endopeptidases; D013194:Staining and Labeling; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D060829:Arthropod Proteins; D004731:Endotoxins; D004792:Enzyme Precursors; D012697:Serine Endopeptidases; C057151:Limulus clotting factor C