Comparison of the binding of nicotinic agonists to receptors from human and rat cerebral cortex and from chick brain (alpha 4 beta 2) transfected into mouse fibroblasts with ion channel activity.
(-)-Nicotine, cytisine and carbachol evoked 86Rb efflux from mouse fibroblasts stably transfected with alpha 4 beta 2 chick brain nicotinic subunits. This response to (-)-nicotine was inhibited by mecamylamine and dihydro-beta-erythroidine and was mirrored by a rise in intracellular Ca2+ measured by microspectrofluorimetry. Lobeline and isoarecolone methiodide evoked no significant 86Rb from cells and unlike the above agonists displayed significantly different IC50 values for the displacement of [3H]nicotine from mammalian (rat and human cerebral cortex) and transfected fibroblast membranes.
Court
J A
JA
MRC Neurochemical Pathology Unit, Newcastle General Hospital, Newcastle upon Tyne, UK.
Perry
E K
EK
Spurden
D
D
Lloyd
S
S
Gillespie
J I
JI
Whiting
P
P
Barlow
R
R
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D002642:Chick Embryo; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism; D016257:Fura-2; D006801:Humans; D007473:Ion Channels / Q000502:physiology; D051379:Mice; D018722:Nicotinic Agonists / Q000378:metabolism*; D051381:Rats; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000378:metabolism*; D012414:Rubidium Radioisotopes
D007473:Ion Channels; D018722:Nicotinic Agonists; D011978:Receptors, Nicotinic; D012414:Rubidium Radioisotopes; D002118:Calcium; D016257:Fura-2