Peptide autoantigenicity of the small nuclear ribonucleoprotein C.
OBJECTIVE
This study determines the antigenic regions of the nRNP C lupus autoantigen.


METHODS
Twenty-one anti-nRNP C sera, six patients with other autoimmune serology, and five normal control sera were assessed for binding with the overlapping octapeptides of the nRNP C protein. Column absorptions were conducted to assess the percentage of the anti-nRNP response which was directed against one major epitode of nRNP C:


RESULTS
Eleven regions of nRNP C are bound in different combinations by anti-nRNP C patient sera. Neither normal controls nor patients without anti-nRNP C antibodies significantly bind any regions of nRNP C: The antigenic region spanning amino acids 117-126, PAPGMRPP, is recognized by all twelve Sm, nRNP precipitin positive patients tested. Indeed, from 14 to 32% of the anti-nRNP reactivity in Sm, nRNP precipitin positive patient sera is directed against PAPGMRPP: All of these patients also bind a very similar sequence which is repeated three times in the Sm B/B' protein. Patients sera with only anti-nRNP antibodies, however, do not recognize this proline rich sequence as antigenic.


CONCLUSIONS
This elucidation of the specific areas of nRNP C which are important in the autoantigenicity of nRNP C will hopefully lead to a better understanding of the involvement of these autoantibodies in the etiology and pathogenesis of SLE.
James
J A
JA
Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA.
Harley
J B
JB
D000595:Amino Acid Sequence; D001323:Autoantibodies; D001324:Autoantigens / Q000032:analysis*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008297:Male; D008969:Molecular Sequence Data; D055517:snRNP Core Proteins
D001323:Autoantibodies; D001324:Autoantigens; D000939:Epitopes; D055517:snRNP Core Proteins