Alteration of neutrophil trafficking by a lipocortin 1 N-terminus peptide.
Sialidase, fucoidin and a peptide corresponding to most of lipocortin 1 N-terminus, termed LC1-(Ac2-26)-peptide, induced an intense 2 h neutrophilia whereas a monoclonal antibody to murine CD11b induced an effect by 1 h. The neutropenic response stimulated by platelet-activating factor (PAF) was significantly reduced in the presence of sialidase, fucoidin, LC1-(Ac2-26)-peptide and monoclonal antibody anti-CD11b. Neutrophil migration into a 6-day-old mouse air-pouch induced by interleukin-1 was inhibited by all the pharmacological agents. In vitro, PAF up-regulated CD11b expression on the neutrophil surface but neither human or mouse LC1-(Ac2-26)-peptide inhibited this response. CD11b up-regulation on neutrophils occurred after PAF administration in vivo and was maximal at 2 min. LC1-(Ac2-26)-peptide mimics the action of agents interfering with leucocyte rolling and adhesion in vivo, however, does not inhibit CD11b up-regulation in vitro suggesting other phenomena are important in the activity of this peptide.
Harris
J G
JG
Department of Biochemical Pharmacology, William Harvey Research Institute, Medical College of St Bartholomew's Hospital, London, UK.
Flower
R J
RJ
Perretti
M
M
D000595:Amino Acid Sequence; D000818:Animals; D017305:Annexin A1 / Q000494:pharmacology*; D000925:Anticoagulants / Q000494:pharmacology; D000954:Antigens, Surface / Q000096:biosynthesis / Q000276:immunology; D018821:CD18 Antigens / Q000096:biosynthesis; D002448:Cell Adhesion / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology; D007700:Kinetics; D008262:Macrophage Activation / Q000187:drug effects; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000201:enzymology; D010455:Peptides / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology
D017305:Annexin A1; D000925:Anticoagulants; D000954:Antigens, Surface; D018821:CD18 Antigens; D016209:Interleukin-8; D016177:Macrophage-1 Antigen; D010455:Peptides; D010972:Platelet Activating Factor; D011134:Polysaccharides; C007789:fucoidan; D009439:Neuraminidase