Changes in the nucleosomal structure of the Marek's disease virus genome in lymphoblastoid cell line MDCC-MSB1 induced by 5-azacytidine.
Marek's disease virus (MDV) DNA in latently infected lymphoblastoid cell lines is considerably methylated. Treatment of the MDV-derived lymphoblastoid cell lines MDCC-MSB1 (MSB1) and MDCC-RP1 (RP1) with 5-azacytidine (5-AzC) results in hypomethylation of MDV DNA. An increase in mRNA from certain portions of MDV DNA, including the BamHI-H region, was observed in 5-AzC-treated MSB1 cells, but not in the agent-treated RP1 cells. After the treatment of cells with 5-AzC, a site hypersensitive to digestion with DNaseI appeared in the BamHI-H region of MDV DNA in MSB1 but not in RP1. These results suggested that the enhancement of mRNA synthesis by 5-AzC is associated with changes in the nucleosomal structure of MDV DNA in lymphoblastoid cell line MSB1.
Hayashi
M
M
Department of Veterinary Radiology, Rakuno Gakuen University, Ebetsu, Japan.
Io
K
K
Furuichi
T
T
Ren
S
S
Isogai
E
E
Watanabe
T
T
Namioka
S
S
D000818:Animals; D001345:Autoradiography; D001374:Azacitidine / Q000494:pharmacology*; D002645:Chickens; D004279:DNA, Viral / Q000187:drug effects; D004587:Electrophoresis, Agar Gel; D008381:Herpesvirus 2, Gallid / Q000187:drug effects* / Q000235:genetics*; D009707:Nucleosomes / Q000187:drug effects / Q000235:genetics; D014407:Tumor Cells, Cultured
D004279:DNA, Viral; D009707:Nucleosomes; D001374:Azacitidine