Up-regulation of nitric oxide synthase by estradiol in human aortic endothelial cells.
We have examined the effects of sex hormones on calcium-dependent NO production and protein levels of NO synthase in cultured human aortic endothelial cells, which were treated with various doses of 17 beta-estradiol and testosterone for 8-48 h. Treatment with 17 beta-estradiol enhanced calcium-dependent NO production, but testosterone had exerted no effect. Western blot using monoclonal anti-human endothelial NO synthase antibody clarified that increased NO production by 17 beta-estradiol treatment was accompanied by increased NO synthase protein. Our results provide evidence that human endothelial NO synthase can be regulated by estrogens.
Hishikawa
K
K
Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.
Nakaki
T
T
Marumo
T
T
Suzuki
H
H
Kato
R
R
Saruta
T
T
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D001011:Aorta; D001483:Base Sequence; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000201:enzymology*; D004790:Enzyme Induction / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D019001:Nitric Oxide Synthase; D012045:Regulatory Sequences, Nucleic Acid; D013629:Tamoxifen / Q000494:pharmacology; D013739:Testosterone / Q000494:pharmacology
D013629:Tamoxifen; D013739:Testosterone; D004958:Estradiol; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases