Cytokine stimulation of CD34+ bone marrow cells prior to cryopreservation enhances their post-thawing proliferative potential.
Marrow aplasia remains a significant cause of morbidity and mortality in the peri-transplant period. Administration of recombinant human hematopoietic growth factors along with the marrow graft is a widely used strategy to ameliorate this problem. Though arguably effective, this approach is extremely expensive. To develop alternative strategies for stimulating marrow engraftment, we investigated the utility of stimulating CD34+ enriched bone marrow cells with cytokines prior to cryopreservation. We found that culturing these cells for 1 to 3 days in Iscove's medium supplemented with kit ligand (KL), interleukin-3 (IL-3), interleukin-1 beta (IL-1 beta) and 20% bovine calf serum before freezing doubled the proliferative capacity of both myeloid and erythroid progenitor cells after thawing. Confirmation of increased proliferative activity in vivo would suggest that this approach might significantly shorten the period of marrow aplasia post transplant at far less expensive means.
Ratajczak
M Z
MZ
Department of Pathology, University of Pennsylvania School of Medicine, Philadelphia.
Ratajczak
J
J
Kregenov
D A
DA
Kuczyński
W I
WI
Skórski
T
T
Gewirtz
A M
AM
D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D014408:Biomarkers, Tumor / Q000032:analysis*; D001853:Bone Marrow / Q000187:drug effects / Q000276:immunology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D015925:Cryopreservation; D016207:Cytokines / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000378:metabolism; D007377:Interleukin-3 / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000276:immunology
D015703:Antigens, CD; D018952:Antigens, CD34; D014408:Biomarkers, Tumor; D016207:Cytokines; D007375:Interleukin-1; D007377:Interleukin-3