Cellular locus of the nitric oxide-synthase involved in cerebellar long-term depression induced by high external potassium concentration.
The cellular location of the NO-synthase involved in long-term depression (LTD) of parallel fiber (PF)-mediated EPSCs induced by raising the external potassium (K) concentration has been investigated by using both whole-cell patch-clamp recordings (WCR) of Purkinje cells (PCs) in thin slices in vitro, and reverse transcription followed by polymerase chain reaction (PCR) applied to mRNAs harvested from these single PCs during WCR. In all tested cells in the control group, a large LTD of PF-mediated EPSCs was induced by perfusing the slices for 3 min with a high (30 mM) K perfusing medium. In a second group of cells for which the protein kinase C (PKC) inhibitor peptide 19-36 was added to the intrapipette solution at a concentration of 10 microM, the LTD following complete wash out of the high K solution was significantly less prominent than in the control group. Very similar results were also obtained when 30 microM NG-methyl-L-arginine (L-NMMA) was added to the perfusing medium. In contrast, when both the PKC inhibitor peptide 19-36 and L-NMMA were added to the intrapipette solution at a concentration of 10 and 30 microM respectively, no LTD was revealed following wash out of the high K solution. Finally, the PCR amplification of mRNAs harvested from these single PCs during WCR, as well as from granule cells from the same slices, confirms that mRNAs encoding the NO-synthase are expressed by granule cells, whereas they are not detected in PCs.
Crepel
F
F
URA CNRS 1121, Bât 440, Université Paris-Sud, Orsay, France.
Audinat
E
E
Daniel
H
H
Hemart
N
N
Jaillard
D
D
Rossier
J
J
Lambolez
B
B
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002531:Cerebellum / Q000166:cytology* / Q000201:enzymology* / Q000502:physiology; D003864:Depression, Chemical; D004594:Electrophysiology; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009473:Neuronal Plasticity / Q000187:drug effects*; D019001:Nitric Oxide Synthase; D018408:Patch-Clamp Techniques; D016133:Polymerase Chain Reaction; D011188:Potassium / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011689:Purkinje Cells / Q000187:drug effects / Q000201:enzymology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D013569:Synapses / Q000187:drug effects
D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D011493:Protein Kinase C; D011188:Potassium