A neutrophil-derived NO-synthase (NOS) inhibitor.
In the present work we have demonstrated that the low NOS activity of circulating blood neutrophils is related to an endogenous inhibitory factor. This factor inhibits constitutive NOS (cNOS) of cerebellum and inducible NOS (iNOS) of macrophages in a concentration-dependent manner. Boiling only partially diminished its activity. The inhibition of cNOS was specific, since to some degree NADPH (0.5-4 mM) and more effectively L-arginine (0.1-1 mM), but not D-arginine, reversed the inhibition.
Dembinska-Kiec
A
A
Department of Clinical Biochemistry, Collegium Medicum, Jagiellonian University, Cracow, Poland.
Burchert
M
M
Hartwich
J
J
Gryglewski
R
R
Peskar
B A
BA
D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors / Q000097:blood* / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D006801:Humans; D017737:Macrophages, Peritoneal / Q000378:metabolism; D008297:Male; D009249:NADP / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology*; D019001:Nitric Oxide Synthase; D051381:Rats
D009249:NADP; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases