Study of the chromium binding in plasma of patients on continuous ambulatory peritoneal dialysis.
The distribution of chromium (Cr) among the proteins in plasma of three continuous ambulatory peritoneal dialysis (CAPD) patients and in dialysis solutions was studied with 'in vitro' and 'in vivo' 51Cr-labelled plasma. Radiolabelling allows the kinetics of newly added Cr to be investigated. The separation of the proteins was achieved by ion exchange fast protein liquid chromatography, ensuring quantitative recoveries of the 51Cr activity, transferrin and albumin. In all fractions the proteins were qualitatively characterised by iso electric focusing. The concentrations of transferrin and albumin were determined by nephelometry. The 51Cr distribution among the plasma proteins was similar for the three patients. The 51Cr activity was mainly bound to transferrin and, in the beginning of the incubation, in a lesser degree also to albumin. After 1-6 h a shift was observed of the 51Cr from albumin to an unidentified low molar mass complex (+/- 5000 Da). Two 51Cr species showed up in the subsequently drained dialysate, but they could not be identified.
Borguet
F
F
Laboratory of Analytical Chemistry, University of Gent, Belgium.
Cornelis
R
R
Delanghe
J
J
Lambert
M C
MC
Lameire
N
N
D000328:Adult; D001798:Blood Proteins / Q000737:chemistry; D002852:Chromatography, Ion Exchange; D002857:Chromium / Q000097:blood* / Q000493:pharmacokinetics; D002860:Chromium Radioisotopes; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008297:Male; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory; D012709:Serum Albumin / Q000032:analysis; D014168:Transferrin / Q000032:analysis; D014511:Uremia / Q000097:blood
D001798:Blood Proteins; D002860:Chromium Radioisotopes; D012709:Serum Albumin; D014168:Transferrin; D002857:Chromium