Immuno-localization of the calcitriol receptor, calbindin-D28k and the plasma membrane calcium pump in the human eye.
Using immunohistochemical methods, we detected epitopes of the calcitriol receptor, the ATP-dependent plasma membrane calcium pump and the 28kD vitamin D-dependent calcium-binding protein in sections of the human eye. In retinal photoreceptors, vitamin D receptor, plasma membrane calcium pump and calcium-binding protein epitopes were detected in the outer nuclear layer. Epitopes for the vitamin D receptor and the calcium-binding protein were present in the inner and outer segments of the photoreceptors, where visual transduction occurs. All three proteins were detected in some cells of the ganglion cell layer, the inner nuclear layer, and the retinal pigment epithelium. Epitopes for these proteins also were noted in the ciliary body epithelium. VDR epitopes were seen in lens epithelium. Some immunostaining for VDR, PMCA and calbindin-D28k also was present in the endothelium and in the basal epithelium of the cornea. The presence of these proteins in several tissues of the human eye suggests that the proteins may play a role in the cellular physiology of the eye. Their exact functions in the eye remain undetermined.
Johnson
J A
JA
Nephrology Research Unit, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
Grande
J P
JP
Roche
P C
PC
Campbell
R J
RJ
Kumar
R
R
D000911:Antibodies, Monoclonal; D064092:Calbindin 1; D064026:Calbindins; D000252:Calcium-Transporting ATPases / Q000032:analysis*; D002462:Cell Membrane / Q000201:enzymology; D000939:Epitopes / Q000032:analysis; D005123:Eye / Q000737:chemistry*; D005136:Eye Proteins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000032:analysis; D018167:Receptors, Calcitriol / Q000032:analysis*; D064030:S100 Calcium Binding Protein G / Q000032:analysis*
D000911:Antibodies, Monoclonal; C576498:CALB1 protein, human; D064092:Calbindin 1; D064026:Calbindins; D000939:Epitopes; D005136:Eye Proteins; D009419:Nerve Tissue Proteins; D018167:Receptors, Calcitriol; D064030:S100 Calcium Binding Protein G; D000252:Calcium-Transporting ATPases