title: Expression of human plasminogen activator inhibitor-2 in the baculovirus expression system.
abstract: A cDNA coding for human plasminogen activator inhibitor-2 (PAI-2) was transferred into the genome of Autographa californica nuclear polyhedrosis virus adjacent to the polyhedrin promoter. Cells infected with recombinant virus synthesized a 43-kDa PAI-2 protein, the majority of which was secreted. The recombinant PAI-2 was identical to native PAI-2 purified from human placenta with respect to interaction with polyclonal antibody and inhibition of urokinase-type plasminogen activator.
authors.affiliation: Department of Biochemistry, Nanjing University, P. R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Pei
authors.forename: A
authors.initials: A
authors.lastname: Zhou
authors.forename: X
authors.initials: X
authors.lastname: Cao
authors.forename: X
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D017396:Plasminogen Activator Inhibitor 2 / Q000378:metabolism* / Q000502:physiology; D018411:Spodoptera / Q000166:cytology / Q000821:virology
chemical_list: D017396:Plasminogen Activator Inhibitor 2