cAMP-activated chloride channels in a CFTR-transfected pancreatic adenocarcinoma-derived cell line, pANS6.
Pancreatic adenocarcinoma cell lines rarely express the CFTR gene, despite the high levels of CFTR protein that are present in primary pancreatic duct cells. We have attempted to generate a non-CF pancreatic adenocarcinoma cell line that stably produces high levels of CFTR mRNA and protein by transfecting a vector containing the CFTR cDNA, driven by a strong mammalian promoter, into the poorly differentiated pancreatic adenocarcinoma cell line, Panc-1. The pANS6 pancreatic duct cell line expresses substantial levels of CFTR mRNA, but little CFTR protein. Despite this we were able to detect low conductance chloride channels in 40% of patches, stimulated with cAMP, that have similar biophysical properties to CFTR.
Smith
A N
AN
Institute of Molecular Medicine, Oxford University, John Radcliffe Hospital, UK.
Wardle
C J
CJ
Winpenny
J P
JP
Verdon
B
B
Gray
M A
MA
Argent
B E
BE
Harris
A
A
D000230:Adenocarcinoma / Q000235:genetics; D002460:Cell Line; D018118:Chloride Channels / Q000737:chemistry / Q000187:drug effects*; D000242:Cyclic AMP / Q000494:pharmacology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D005822:Genetic Vectors; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D010190:Pancreatic Neoplasms / Q000235:genetics; D018408:Patch-Clamp Techniques; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection; D014407:Tumor Cells, Cultured
D018118:Chloride Channels; D008565:Membrane Proteins; D012333:RNA, Messenger; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D000242:Cyclic AMP