title: Myeloglycan, a series of E-selectin-binding polylactosaminolipids found in normal human leukocytes and myelocytic leukemia HL60 cells.
abstract: Sialosyl-Lex (SLex) is assumed to be the binding epitope of E- and P-selectin in normal human neutrophils and myelocytic leukemia HL60 cells. Glycosphingolipid (GSL) fractions from large quantities of normal human neutrophils and HL60 cell extract did not contain SLex GSLs having 6-10 sugar residues, as commonly found in solid tumor cells and tissues. Instead, the binding target of E-selectin was revealed to be a series of long-chain, unbranched polylactosamine GSLs with terminally sialylated, internally alpha 1-->3 polyfucosylated structure as the major component, or having SLex at the terminus and internally polyfucosylated structure as a minor component. These GSLs are hereby collectively termed "myeloglycan." Regardless of the site of fucosylation, all myeloglycans cross-react strongly with "anti-SLex" monoclonal antibodies such as CSLEX, FH6, SNH3, and SNH4.
authors.affiliation: Biomembrane Institute, Seattle, WA 98119, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Stroud
authors.forename: K
authors.initials: K
authors.lastname: Handa
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: M E
authors.initials: ME
authors.lastname: Salyan
authors.forename: H
authors.initials: H
authors.lastname: Fang
authors.forename: S B
authors.initials: SB
authors.lastname: Levery
authors.forename: S
authors.initials: S
authors.lastname: Hakamori
authors.forename: B B
authors.initials: BB
authors.lastname: Reinhold
authors.forename: V N
authors.initials: VN
authors.lastname: Reinhold
mesh_terms: D000117:Acetylglucosamine / Q000032:analysis; D000818:Animals; D000911:Antibodies, Monoclonal; D001665:Binding Sites; D016466:CHO Cells; D002240:Carbohydrate Sequence; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000378:metabolism*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D019040:E-Selectin; D000939:Epitopes / Q000032:analysis; D006001:Glycoconjugates / Q000737:chemistry / Q000302:isolation & purification; D006028:Glycosphingolipids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000378:metabolism*; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D000939:Epitopes; D006001:Glycoconjugates; D006028:Glycosphingolipids; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D011994:Recombinant Proteins; D000117:Acetylglucosamine