Tumour angiogenesis and tumour cell proliferation as prognostic indicators in gastric carcinoma.
Tumour growth depends on neovascularisation and tumour cell proliferation. Factor VIII-related antigen (F-VIII RA) localises to vascular endothelium. Expression of proliferating cell nuclear antigen (PCNA) is correlated with cell proliferation. We investigated the correlation between the expression of these antigens and prognosis in gastric carcinoma. A total of 108 specimens resected from patients with gastric carcinoma were investigated by staining with monoclonal antibodies against F-VIII RA and PCNA. Microvessel count (MVC; the mean number of microvessels in the five areas of highest vascular density at 200 x magnification) and PCNA labelling index (PCNA LI; percentage of positive cells in more than 500 tumour cells) were determined. The results showed that prognosis was significantly worse in patients who had a tumour with a high MVC (16 or greater) or a high PCNA LI (42% or greater) than in those patients who had a tumour with a low MVC (less than 16) or a low PCNA LI (less than 42%). Furthermore, MVC was significantly associated with the risk of hepatic recurrence. In conclusion, both MVC and PCNA LI may be good prognostic indicators in patients with gastric carcinoma.
Maeda
K
K
First Department of Surgery, Osaka City University Medical School, Japan.
Chung
Y S
YS
Takatsuka
S
S
Ogawa
Y
Y
Onoda
N
N
Sawada
T
T
Kato
Y
Y
Nitta
A
A
Arimoto
Y
Y
Kondo
Y
Y
D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis; D002455:Cell Division / Q000502:physiology; D004730:Endothelium, Vascular / Q000737:chemistry; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000503:physiopathology*; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000098:blood supply* / Q000737:chemistry / Q000473:pathology*; D016019:Survival Analysis; D014841:von Willebrand Factor / Q000032:analysis
D000951:Antigens, Neoplasm; D018809:Proliferating Cell Nuclear Antigen; D014841:von Willebrand Factor