Depression of fast axonal transport in axons demyelinated by intraneural injection of a neurotoxin from K. humboldtiana.
Tullidinol, a neurotoxin extracted from the Karwinskia humboldtiana fruit, dissolved in peanut oil was injected into the right sciatic nerve of adult cats. The contralateral sciatic nerve received an equivalent volume of peanut oil alone. The fast axonal transport of labeled ([3H]Leucine) protein was studied in sensory and motor axons of both sciatic nerves. The radioactive label was pressure injected either into the L7 dorsal root ganglion or the ventral region of the same spinal cord segment. Several days after the toxin injection, the cat limped and the Achilles tendon reflex was nearly absent in the right hind limb. The amount of transported label was decreased distal to the site of toxin injection. Proximal to this site, the transported material was damned. Sensory and motor axons showed similar changes. In addition, the toxin produced demyelination and axonal degeneration. Axonal transport and the structure of the axons were normal in the contralateral nerve. Both, Schwann cells and axons of the right sciatic nerve showed globular inclusions, presumably oil droplets containing the toxin. We conclude that Schwann cells and axons as well are tullidinol targets.
Muñoz-Martínez
E J
EJ
Departmento de Fisiología, Biofisica y Neurociencias, Centro de Investigación y de Estudios Avanzados del IPN, México, D.F.
Cuéllar-Pedroza
L H
LH
Rubio-Franchini
C
C
Jáuregui-Rincón
J
J
Joseph-Nathan
P
P
D000125:Achilles Tendon / Q000187:drug effects; D000818:Animals; D000873:Anthracenes / Q000633:toxicity*; D001370:Axonal Transport / Q000187:drug effects*; D001369:Axons / Q000187:drug effects* / Q000502:physiology* / Q000648:ultrastructure; D002415:Cats; D008297:Male; D009498:Neurotoxins / Q000633:toxicity*; D010947:Plants, Toxic; D011714:Pyrans / Q000633:toxicity*; D012018:Reflex / Q000187:drug effects; D012583:Schwann Cells / Q000187:drug effects / Q000473:pathology; D012584:Sciatic Nerve / Q000187:drug effects / Q000473:pathology / Q000502:physiology*
D000873:Anthracenes; D009498:Neurotoxins; D011714:Pyrans; C075148:Karwinskia toxin T-544