Methods for construction of adenovirus vectors.
Adenoviruses are attracting increasing attention as general purpose mammalian cell expression vectors, as recombinant vaccines, and potentially as vectors for gene therapy. Not only is the adenovirus genome relatively easy to manipulate by recombinant DNA techniques, but adenovirus vectors are relatively stable, grow to high titers, and can transduce a variety of cell types in cell culture and in vivo. Vectors can be designed that are either replication competent or replication defective and, in the latter case, are highly efficient at delivering and expressing genes in mammalian cells without resulting in cell killing. Methods are described for growing, titrating, and purifying adenoviruses, for extracting viral DNA from purified virions and from infected cells, for rescuing inserts of foreign DNA into the viral genome, and for assessing expression of inserted genes in adenovirus vectors.
Graham
F L
FL
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
Prevec
L
L
D000256:Adenoviridae / Q000235:genetics*; D018014:Gene Transfer Techniques; D005821:Genetic Techniques; D005822:Genetic Vectors