Differentiation of B16 murine melanoma cells is associated with an increased level of c-SRC.
Elevated levels of c-SRC activity have been found in human melanocytes and some human melanoma cell lines. We examined c-SRC in B16 murine melanoma cells. B16-F0 non-metastatic melanoma cells contained threefold more c-SRC activity and protein than NIH 3T3 murine fibroblasts. Differentiation of B16-F1 metastatic melanoma cells with retinoic acid resulted in an elevation in c-SRC activity, protein and mRNA. The increase in c-SRC was detectable after about 48 h of retinoic acid treatment, as were changes in cellular morphology and growth rate. Thus, there is a correlation between differentiation and expression of c-SRC in B16 murine melanoma cells. These findings suggest a role for c-SRC in murine melanocyte differentiation or function.
O'Connor
T J
TJ
Cell Regulation Research Group, University of Calgary, Alberta, Canada.
Fujita
D J
DJ
D016475:3T3 Cells; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D005347:Fibroblasts; D008544:Melanocytes; D008546:Melanoma, Experimental / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000032:analysis; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D012334:RNA, Neoplasm; D014212:Tretinoin; D016392:Proto-Oncogene Proteins pp60(c-src)