Cytotoxic effects of several hopanoids on mouse leukemia L1210 and P388 cells.
The cytotoxic effects of hopanoids, including bacteriohopane-32, 33, 34, 35-tetrol (Tetrol), bacteriohopane-32-ol (Monol), diploptene, diplopterol and acetylated Monol (AcO-Monol) isolated from Acetobacter aceti, were tested against two leukemia cell lines. Tetrol and Monol have been shown to be toxic to mouse L1210 and P388 compared to the other hopanoids. By measuring the ESR spectra of the spin labeled membranes of these cells, it was shown that the incorporation of Monol resulted in a decrease in the fluidity of the membranes. The decrease in membrane fluidity may correlate, in part, with the cytotoxicity of hopanoids against the two cell lines.
Chen
Z
Z
Faculty of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
Washio
T
T
Sato
M
M
Suzuki
Y
Y
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D007939:Leukemia L1210 / Q000188:drug therapy* / Q000378:metabolism; D007941:Leukemia P388 / Q000188:drug therapy* / Q000378:metabolism; D008560:Membrane Fluidity / Q000187:drug effects; D051379:Mice; D008811:Mice, Inbred DBA; D010101:Oxygen Consumption / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles; D014315:Triterpenes / Q000494:pharmacology*
D000970:Antineoplastic Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D014315:Triterpenes; C022616:thiazolyl blue