A splice donor site mutation results in the insertion of five extra amino acids into P53 from SEWA mouse sarcoma cells.
The status of the p53 gene in SEWA-AS12-ADH (S-ADH) cells, a subline of the mouse sarcoma cell line SEWA, was examined. Immunoprecipitation with wild-type (wt) or mutant P53-specific monoclonal antibodies (mAb) showed that both wt and mutant P53 were produced. Sequence analysis of the p53 cDNA and genomic DNA revealed a single nucleotide (nt) substitution at a splice donor site at the beginning of intron 7. As a result of this mutation, an alternative splice site 15 nt further 3' in intron 7 is used. The P53 protein translated from this aberrantly spliced mRNA carries an Arg258-->Ser substitution, followed by an insertion of 5 extra amino acids. This is the first example of a splice-site mutation in the mouse p53 gene.
Magnusson
K P
KP
Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
Minarovits
J
J
Klein
G
G
Wiman
K G
KG
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016158:Genes, p53; D066298:In Vitro Techniques; D051379:Mice; D008969:Molecular Sequence Data; D017354:Point Mutation; D012513:Sarcoma, Experimental / Q000235:genetics*; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D016159:Tumor Suppressor Protein p53