title: CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis.
abstract: Centromere protein-F (CENP-F) is mammalian kinetochore protein that was recently identified by an autoimmune serum (Rattner, J. B., A. Rao, M. J. Fritzler, D. W. Valencia, and T. J. Yen. Cell Motil. Cytoskeleton. 26:214-226). We report here the human cDNA sequence of CENP-F, along with its expression and localization patterns at different stages of the HeLa cell cycle. CENP-F is protein of the nuclear matrix that gradually accumulates during the cell cycle until it reaches peak levels in G2 and M phase cells and is rapidly degraded upon completion of mitosis. CENP-F is first detected at the prekinetochore complex during late G2, and is clearly detectable as paired foci that correspond to all the centromeres by prophase. During mitosis, CENP-F is associated with kinetochores from prometaphase until early anaphase and is then detected at the spindle midzone throughout the remainder of anaphase. By telophase, CENP-F is concentrated within the intracellular bridge at either side of the mid-body. The predicted structure of the 367-kD CENP-F protein consists of two 1,600-amino acid-long coil domains that flank a central flexible core. A putative P-loop nucleotide binding site (ADIPTGKT) is located within the globular carboxy terminus. The structural features deduced from our sequence studies and the spatial and temperal distribution of CENP-F revealed in our cytological and biochemical studies suggest that it may play a role in several mitotic events.
authors.affiliation: Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, Pennsylvania 19111, USA.
authors.forename: H
authors.initials: H
authors.lastname: Liao
authors.forename: R J
authors.initials: RJ
authors.lastname: Winkfein
authors.forename: G
authors.initials: G
authors.lastname: Mack
authors.forename: J B
authors.initials: JB
authors.lastname: Rattner
authors.forename: T J
authors.initials: TJ
authors.lastname: Yen
mesh_terms: D000595:Amino Acid Sequence; D034961:Antigens, Nuclear; D001323:Autoantibodies / Q000097:blood; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002451:Cell Compartmentation; D002455:Cell Division / Q000502:physiology*; D002467:Cell Nucleus / Q000648:ultrastructure; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D003429:Cross Reactions; D018076:DNA, Complementary / Q000235:genetics; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D016195:G2 Phase / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D018386:Kinetochores / Q000378:metabolism*; D008840:Microfilament Proteins; D008938:Mitosis / Q000502:physiology; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000737:chemistry / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017422:Sequence Analysis, DNA
chemical_list: D034961:Antigens, Nuclear; D001323:Autoantibodies; D001324:Autoantigens; D002868:Chromosomal Proteins, Non-Histone; D018076:DNA, Complementary; D000939:Epitopes; D008840:Microfilament Proteins; D009687:Nuclear Proteins; C094772:centromere protein F