title: Accumulation of isogloboside and ganglio-N-tetraosyl ceramide having blood group B determinant in the hepatomas of female LEC rats.
abstract: We have studied the neutral glycolipid composition of spontaneous hepatomas in LEC female rats. Neutral lipid fractions were isolated and purified by column chromatographies on DEAE-Toyopearl 650(M) and Iatrobeads. The neutral glycolipid fraction contained 3.2 to 4.4 micrograms lipid-bound glucose (Glc) per mg protein, and consisted of isogloboside (iso-Gb4, 50.8% of total neutral glycolipids) and IV3Gal, IV2Fuc, GgOse4Cer (asialo-BGM1, 13.5%) as the major neutral glycolipids and Gb3 and iso-Gb3 (9.2%), GlcCer (7.2%), LacCer (6.1%) as the other species. The structure of iso-Gb4 was elucidated by gas-liquid chromatography (GLC), permethylation study, liquid secondary ion (LSI) mass spectrometry, and nuclear Overhauser enhancement spectroscopy (NOESY) and that for asialo-BGM1 by GLC, LSI mass spectrometry, and high-performance thin-layer chromatography (HPTLC)-overlay method using anti-asialo-BGM1 antibody. Isogloboside and asialo-BGM1 which are found in negligible amounts in normal liver tissues may represent excellent markers for studying tumor metastasis and cellular adhesion.
authors.affiliation: Department of Biochemistry and Molecular Biophysics, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
authors.forename: T
authors.initials: T
authors.lastname: Ariga
authors.forename: N
authors.initials: N
authors.lastname: Kasai
authors.forename: I
authors.initials: I
authors.lastname: Miyoshi
authors.forename: M
authors.initials: M
authors.lastname: Yamawaki
authors.forename: J N
authors.initials: JN
authors.lastname: Scarsdale
authors.forename: R K
authors.initials: RK
authors.lastname: Yu
authors.forename: T
authors.initials: T
authors.lastname: Kasama
authors.forename: T
authors.initials: T
authors.lastname: Taki
mesh_terms: D000017:ABO Blood-Group System; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D002240:Carbohydrate Sequence; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D000939:Epitopes / Q000032:analysis; D005260:Female; D005677:G(M1) Ganglioside / Q000276:immunology / Q000378:metabolism*; D005915:Globosides / Q000276:immunology / Q000378:metabolism*; D006017:Glycolipids / Q000302:isolation & purification / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats
chemical_list: D000017:ABO Blood-Group System; D014408:Biomarkers, Tumor; D000939:Epitopes; D005915:Globosides; D006017:Glycolipids; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside