Famotidine, a histamine-2-receptor antagonist, inhibits the increase in rat gastric H+/K(+)-ATPase mRNA induced by intravenous infusion of gastrin 17 and histamine.
We examined the effects of gastrin and histamine on rat gastric H+/K(+)-ATPase, the enzyme responsible for H+ secretion, gene expression in vivo. Gastrin 17 (G 17) or histamine dihydrochloride (histamine) was continuously infused through the femoral vein of anesthetized rats. Gastric H+/K(+)-ATPase mRNA levels were measured using northern blot analysis. Infusion of G 17 and histamine increased the H+/K(+)-ATPase mRNA level significantly compared with basal control level or vehicle control level (P < 0.01). However, pretreatment with famotidine, a potent histamine-2 (H2)-receptor antagonist, inhibited the increase of rat gastric H+/K(+)-ATPase mRNA following G 17 and histamine infusion. These findings indicate that both histamine and G 17 increase expression of H+/K(+)-ATPase mRNA by activating H2 receptor on the parietal cell.
Yamamoto
G
G
First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Tari
A
A
Sumii
K
K
Sumii
M
M
Haruma
K
K
Kajiyama
G
G
D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D015738:Famotidine / Q000494:pharmacology*; D005755:Gastrins / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D017506:H(+)-K(+)-Exchanging ATPase / Q000096:biosynthesis* / Q000235:genetics; D006632:Histamine / Q000494:pharmacology*; D006635:Histamine H2 Antagonists / Q000494:pharmacology*; D006728:Hormones / Q000494:pharmacology*; D007262:Infusions, Intravenous; D008297:Male; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000201:enzymology*; D011292:Premedication; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D011970:Receptors, Histamine H2 / Q000502:physiology*
D000199:Actins; D005755:Gastrins; D006635:Histamine H2 Antagonists; D006728:Hormones; D012333:RNA, Messenger; D011970:Receptors, Histamine H2; D015738:Famotidine; C013550:gastrin 17; D006632:Histamine; D017506:H(+)-K(+)-Exchanging ATPase