Molecular cloning and expression in Escherichia coli of bleomycin-resistance gene from a methicillin-resistant Staphylococcus aureus and its association with IS431 mec.
A gene that confers bleomycin resistance was cloned from the chromosomal DNA of methicillin-resistant Staphylococcus aureus (MRSA) B-26 into the plasmid pUC18. It is of chromosomal origin rather than plasmid and exists in the chromosome making a cluster with the kanamycin-resistance gene. We found that the nucleotide sequence of the bleomycin-resistance gene from the chromosome of MRSA B-26 is identical to that from a staphylococcal plasmid, pUB110. The partial sequence of IS431mec was also found upstream from the DNA fragment containing the bleomycin- and kanamycin-resistance genes.
Bhuiyan
M Z
MZ
Department of Paediatrics, Hiroshima University School of Medicine, Japan.
Ueda
K
K
Inouye
Y
Y
Sugiyama
M
M
D001483:Base Sequence; D001761:Bleomycin / Q000494:pharmacology*; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D007613:Kanamycin Resistance / Q000235:genetics; D016106:Methicillin Resistance; D008969:Molecular Sequence Data; D005810:Multigene Family; D013211:Staphylococcus aureus / Q000235:genetics*
D001761:Bleomycin