Involvement of protein kinase C in the delayed cytoprotection following sublethal ischaemia in rabbit myocardium.
Rabbit hearts were preconditioned with four 5 min coronary artery occlusions 24 h before 30 min coronary occlusion with 120 min reperfusion. Preconditioning significantly reduced the percentage of myocardium infarcting within the risk zone from 49.1 +/- 4.3% to 31.8 +/- 3.5% (P < 0.05). When the protein kinase C (PKC) inhibitor, chelerythrine, was administered just before preconditioning, the delayed protection against infarction 24 h later was abolished. We conclude that the delayed cytoprotective response associated with ischaemic preconditioning of myocardium is likely to involve the early activation of one or more PKC subtypes.
Baxter
G F
GF
Hatter Institute for Cardiovascular Studies, University College London Hospital and Medical School.
Goma
F M
FM
Yellon
D M
DM
D000470:Alkaloids; D000704:Analysis of Variance; D000818:Animals; D053119:Benzophenanthridines; D001794:Blood Pressure / Q000187:drug effects; D002104:Cadmium / Q000378:metabolism; D019187:Cadmium Compounds; D003327:Coronary Disease / Q000503:physiopathology; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000187:drug effects; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D008863:Microspheres; D017202:Myocardial Ischemia / Q000188:drug therapy / Q000201:enzymology*; D010617:Phenanthridines / Q000494:pharmacology* / Q000627:therapeutic use; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011817:Rabbits; D013194:Staining and Labeling; D013440:Sulfides; D013778:Tetrazolium Salts / Q000737:chemistry; D015032:Zinc / Q000378:metabolism
D000470:Alkaloids; D053119:Benzophenanthridines; D019187:Cadmium Compounds; D010617:Phenanthridines; D013440:Sulfides; D013778:Tetrazolium Salts; D002104:Cadmium; C034939:cadmium sulfide; C009591:triphenyltetrazolium; C016299:chelerythrine; D011493:Protein Kinase C; D015032:Zinc