Separation of antigenic glycoprotein fractions from cell-free homogenate of Pseudomonas pseudomallei and characterization as tyrosine phosphatase.
Cell-free extracts were prepared from Pseudomonas pseudomallei cells by freezing-thawing, sonication, and differential ultracentrifugation. The extracts were subjected to column chromatography with DEAE-sepharose to obtain glycoprotein fractions. The fractions showed acid phosphatase activity to p-nitrophenyl phosphate, tyrosine phosphate, serine phosphate, but not to threonine phosphate. They were highly antigenic when tested by immunofluorescence assay with the sera of melioidosis patients.
Kondo
E
E
National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand.
Wangroongsaub
P
P
Naigowit
P
P
Kanai
K
K
D000818:Animals; D016957:Burkholderia pseudomallei / Q000201:enzymology*; D002474:Cell-Free System; D005591:Chemical Fractionation; D002847:Chromatography, Agarose; D006801:Humans; D017027:Protein Tyrosine Phosphatases / Q000302:isolation & purification*; D014461:Ultracentrifugation
D017027:Protein Tyrosine Phosphatases