Alterations of cell surface antigens induced by placental isoform of ferritin in human carcinoma cell lines.
OBJECTIVE
the ability of the breast cancer-associated placental acid isoform of ferritin (p43-PLF) to modulate cell surface expression of HLA, CD59 (protectin) and CD66 antigen (adhesion antigen related to CEA) was examined.


METHODS
the expression of these antigens in human breast carcinoma cell lines BT-20, T47D and MDA-MB-468 was determined with the aid of flow cytometry and monoclonal antibodies.


RESULTS
PLF induced a transient up-regulation followed by a down regulation of cell surface protectin (CD59 antigen) on the cell surface of T47D and to a lesser extent, BT-20 human breast carcinoma cell lines. Furthermore, PLF down-regulated cell surface expression of CEA-related CD66 antigen on both these cell lines. No PLF-induced alterations of protectin, CD66 antigen and HLA class I antigen were found on the MDA-MB-468 breast cancer cell line.


CONCLUSIONS
breast cancer-associated p43 induces alterations of the expression of cell surface molecules in breast cancer cells which could have an effect on the modulation of cancer cell adhesive interactions.
Sedlak
J
J
Cancer Research Institute, Bratislava, Slovakia.
Reinerova
M
M
Hunakova
L
L
Ausch
C
C
Chorvath
B
B
Rosen
H
H
Moroz
C
C
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015703:Antigens, CD / Q000187:drug effects* / Q000378:metabolism; D000943:Antigens, Differentiation / Q000187:drug effects* / Q000378:metabolism; D000951:Antigens, Neoplasm / Q000187:drug effects* / Q000378:metabolism; D000954:Antigens, Surface / Q000187:drug effects* / Q000378:metabolism; D001943:Breast Neoplasms / Q000276:immunology*; D018957:CD59 Antigens; D015815:Cell Adhesion Molecules; D015536:Down-Regulation; D005293:Ferritins / Q000276:immunology / Q000494:pharmacology*; D006680:HLA Antigens / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008562:Membrane Glycoproteins / Q000187:drug effects* / Q000378:metabolism; D010920:Placenta; D014407:Tumor Cells, Cultured; D015854:Up-Regulation
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D018957:CD59 Antigens; C069847:CD66 antigens; D015815:Cell Adhesion Molecules; D006680:HLA Antigens; D008562:Membrane Glycoproteins; D005293:Ferritins