The bldA-encoded tRNA is poorly expressed in the bldI mutant of Streptomyces coelicolor A3(2).
The bldA gene, encoding a leucyl tRNA recognizing the UUA codon, is expressed at significantly lower levels in the bldI mutant, Streptomyces coelicolor J703, than in the parent S. coelicolor A3(2). Expression of a TTA-containing reporter gene was reduced in the bldI mutant, as was the mature, 87 nucleotide, form of the bldA-encoded tRNA. This reduced level of the tRNA was also seen when the bldA gene was introduced on a high-copy-number plasmid into the bldI mutant, suggesting that maximal bldA expression may require a bldI-dependent promoter.
Leskiw
Brenda K
BK
Mah
Richard
R
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015152:Blotting, Northern; D018628:Gene Dosage; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D008969:Molecular Sequence Data; D009154:Mutation; D010957:Plasmids; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012356:RNA, Transfer, Leu / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D013170:Spores; D013302:Streptomyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D012356:RNA, Transfer, Leu