The interaction of TFIIIA with specific RNA-DNA heteroduplexes.
We examine the association of transcription factor TFIIIA with RNA-DNA heteroduplexes containing sequences from the Xenopus borealis 5 S rRNA gene. Under conditions where TFIIIA selectively binds to 5 S rRNA or the internal control region of the 5 S rRNA gene, no specific association of TFIIIA with DNA-RNA heteroduplexes containing either strand of 5 S DNA could be detected. We discuss our results with respect to specific models of TFIIIA recognition of the internal control region and of DNA-RNA hybrids by zinc finger proteins.
Nightingale
K P
KP
Laboratory of Molecular Embryology, NICHD, National Institutes of Health, Bethesda, Maryland 20892-2710, USA.
Wolffe
A P
AP
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000302:isolation & purification / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D007700:Kinetics; D008969:Molecular Sequence Data; D009692:Nucleic Acid Heteroduplexes / Q000302:isolation & purification / Q000378:metabolism*; D016372:RNA, Antisense / Q000302:isolation & purification / Q000378:metabolism; D012341:RNA, Ribosomal, 5S / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*; D014981:Xenopus; D016335:Zinc Fingers
D017931:DNA Primers; D004275:DNA, Ribosomal; D004268:DNA-Binding Proteins; D009692:Nucleic Acid Heteroduplexes; D016372:RNA, Antisense; D012341:RNA, Ribosomal, 5S; D035582:Transcription Factor TFIIIA; D014157:Transcription Factors