title: Biochemical and molecular characterization of rhesus monkey major histocompatibility complex class II DR.
abstract: Rapid, simple techniques for MHC class II typing were explored to facilitate the use of a wide variety of nonhuman primate species as models of human diseases and therapies. We demonstrate that radioimmunoprecipitation and 1-D IEF or 1-D NEPHGE can be employed for characterizing MHC class II DR alleles in rhesus monkeys. Complementary molecular analyses have yielded the first full-length nonhuman primate DRA sequence and the first full-length rhesus macaque DRB sequences. In this way it has also been possible to determine which subset of the DRB sequences amplified from a B-LCL are actually expressed.
authors.affiliation: Harvard Medical School, New England Regional Primate Research Center, Southborough, Massachusetts, USA.
authors.forename: C
authors.initials: C
authors.lastname: Lekutis
authors.forename: N L
authors.initials: NL
authors.lastname: Letvin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005802:Genes, MHC Class II; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000276:immunology; D008253:Macaca mulatta / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
chemical_list: D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II