Spatial discontinuities in human immunodeficiency virus type 1 quasispecies derived from epidermal Langerhans cells of a patient with AIDS and evidence for double infection.
A nonhomogeneous spatial distribution of human immunodeficiency virus type 1 quasispecies was observed for epidermal Langerhans cells purified from skin patches taken from a patient with AIDS soon after death. Each patch presented a unique collection of sequences, distinct from those of juxtaposed patches or those derived from the other leg. Infection of Langerhans cells by virus from underlying T cells in the dermis might explain this partition. The analysis revealed the presence of two distinct cocirculating viral strains, indicating double infection.
Sala
M
M
Unité de Rétrovirologie Moléculaire, Institut Pasteur, Paris, France.
Zambruno
G
G
Vartanian
J P
JP
Marconi
A
A
Bertazzoni
U
U
Wain-Hobson
S
S
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral; D015497:HIV-1 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007515:Islets of Langerhans / Q000382:microbiology*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D004279:DNA, Viral