Evidence for close in vivo association of protein with mitochondrial ribosomal RNA in a trypanosome.
Riboprotein particles containing the ribosomal RNA-like 9S and 12S RNAs in the trypanosome mitochondrion have never been isolated. Using the formaldehyde cross-linking procedure I show here that one or more of three proteins, q2 (16.5 kDa), r (15 kDa) and s (13 kDa), are closely associated in vivo with the 9S and 12S RNAs of the trypanosomatid parasite, Crithidia fasciculata. These proteins were also found to be associated with the parasite's cytosolic ribosomal RNA and to have strong immunological cross reactivity with riboprotein S11 of the bacterium Escherichia coli. These data provide the first evidence for the association of riboprotein-like proteins with the 9S and 12S mitochondrial RNA in a trypanosome, possibly as components of a mitochondrial ribosome.
Tittawella
I
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Department of Cell- & Molecular Biology, University of Umeå, Sweden.
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002451:Cell Compartmentation; D016831:Crithidia fasciculata / Q000737:chemistry* / Q000166:cytology; D003432:Cross-Linking Reagents; D003600:Cytosol / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D008928:Mitochondria / Q000737:chemistry*; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis / Q000235:genetics; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000032:analysis* / Q000235:genetics; D016601:RNA-Binding Proteins / Q000032:analysis* / Q000302:isolation & purification; D012261:Ribonucleoproteins / Q000737:chemistry*
D003432:Cross-Linking Reagents; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; C075526:RNA, ribosomal, 12S; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D005557:Formaldehyde; D012313:RNA