Na,K-ATPase beta subunit isoform expression in the peripheral nervous system of the rat.
Using the RNase protection assay (RPA) to study the distribution of isoforms of the non-catalytic (beta) subunit of Na,K-ATPase in the peripheral nervous system, we found both beta 1 and beta 2 isoform mRNAs in dorsal root ganglion (DRG), but only beta 2 mRNA in sciatic nerve. Using Western blot to measure accumulation of the polypeptides at a ligature on the nerve we found that beta 1 but not beta 2 polypeptide is carried by rapid axonal transport in the sciatic nerve. These results imply that beta 1 is the prominent isoform of Na,K-ATPase in neurons and beta 2 the prominent isoform in Schwann cells.
Fink
D J
DJ
Department of Neurology, University of Michigan, Ann Arbor, USA.
Fang
D
D
Li
T
T
Mata
M
M
D000818:Animals; D001370:Axonal Transport / Q000502:physiology; D001483:Base Sequence; D015153:Blotting, Western; D003720:Densitometry; D005727:Ganglia, Spinal / Q000166:cytology / Q000201:enzymology; D007527:Isoenzymes / Q000096:biosynthesis*; D007700:Kinetics; D008297:Male; D008969:Molecular Sequence Data; D017933:Peripheral Nervous System / Q000201:enzymology*; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000201:enzymology; D012584:Sciatic Nerve / Q000201:enzymology; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis*
D007527:Isoenzymes; D012313:RNA; D000254:Sodium-Potassium-Exchanging ATPase