Mouse p87wee1 kinase is regulated by M-phase specific phosphorylation.
We have cloned a mouse wee1 kinase cDNA (mwee1). The clone is 2258 bp in length and its open reading frame corresponds to 646 amino acid residues. The molecular weight of this kinase is 87 kDa in SDS-PAGE, which is about 1.7-fold larger than the human p50wee1 kinase reported previously. In a cell cycle, the mouse wee1 kinase is phosphorylated at M-phase, and an in vitro study using a mitotic extract revealed that phosphorylation occurs in the N-terminal domain, which is absent from the human wee1 kinase, resulting in inactivation of the kinase activity. The N-terminal domain or entire molecule is extensively phosphorylated by cdc2-cyclin B kinase. Furthermore, the activity of the wee1 kinase was reduced by phosphorylation with the mitotic extract which contained cdc2-cyclin B kinase.
Honda
R
R
School of Life Science, Tokyo University of Pharmacy and Life Science, Japan.
Tanaka
H
H
Ohba
Y
Y
Yasuda
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004988:Ethers, Cyclic / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D051379:Mice; D008938:Mitosis; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D019319:Okadaic Acid; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D004988:Ethers, Cyclic; D009687:Nuclear Proteins; D019319:Okadaic Acid; D011505:Protein-Tyrosine Kinases; C499349:WEE1 protein, human; C499348:Wee1 protein, mouse; D010749:Phosphoprotein Phosphatases