title: Trypanosoma brucei brucei and T. b. gambiense: stumpy bloodstream forms express more CB1 epitope in endosomes and lysosomes than slender forms.
abstract: CB1-glycoprotein is a component of flagellar pocket, endosome, and lysosome membranes of long, slender bloodstream forms of the Trypanosoma brucei subgroup of African trypanosomes. We have used immunoblotting, immunofluorescence, and cryoimmunoelectron microscopy to study CB1-glycoprotein expression as long, slender bloodstream forms of pleomorphic T. b. brucei and T. b. gambiense transform through intermediate stages into short, stumpy forms. Intermediate and stumpy forms express more CB1-glycoprotein than long, slender forms. These results, coupled with previous work showing that procyclic forms do not express CB1-glycoprotein, show that the expression of lysosomal membrane glycoproteins is regulated coordinately with other aspects of lysosome and endosome function as these trypanosomes go through their life cycle.
authors.affiliation: Department of Immunology, Duke University Medical Center, Durham, North Carolina 27710.
authors.forename: M J
authors.initials: MJ
authors.lastname: Brickman
authors.forename: A E
authors.initials: AE
authors.lastname: Balber
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D011992:Endosomes / Q000737:chemistry; D000939:Epitopes / Q000032:analysis; D005407:Flagella / Q000737:chemistry; D006023:Glycoproteins / Q000032:analysis*; D008247:Lysosomes / Q000737:chemistry / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016253:Microscopy, Immunoelectron; D014346:Trypanosoma brucei brucei / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D014347:Trypanosoma brucei gambiense / Q000276:immunology / Q000502:physiology* / Q000648:ultrastructure; D014353:Trypanosomiasis, African / Q000469:parasitology
chemical_list: D000953:Antigens, Protozoan; D000939:Epitopes; D006023:Glycoproteins