title: Human IgG subclass responses and subclass restriction to Schistosoma mansoni egg antigens.
abstract: In areas endemic for schistosomiasis, there is great heterogeneity in antibody isotype responses to parasite antigens amongst infected individuals. At the population level, the isotype composition of antibody responses undergoes dynamic changes which are associated with the age of infected individuals. Here we examine the IgG subclass responses to Schistosoma mansoni eggs (soluble egg antigens; SEA) of infected individuals by immunoblot and ELISA. By controlled treatment of SEA-coated ELISA plates and immunoblot nitrocellular strips with sodium periodate, in order to oxidize terminal carbohydrate residues selectively, we were able to relate individuals subjects' isotype responses to the different antigens that they responded to, and to the presence of putative carbohydrate and peptide epitopes on those antigens. IgG2 responses were restricted strictly to sodium periodate-sensitive carbohydrate epitopes and antigens of relatively high molecular weight. These antigens were not usually recognized by other isotypes and, therefore, they were only recognized by individuals who had high levels of IgG2. IgG1 and IgG3 responses were directed against both carbohydrate and peptide epitopes, whereas IgG4 responses were restricted to periodate-resistant epitopes. This suggests that the fall in IgG2 responses, and reciprocal rise in IgG4 antibodies, seen in young children as their intensities of schistosome infection increase, is not the result of isotype switching, and that, if these two subclasses are involved in blocking immunity to schistosomiasis, they are operating independently.
authors.affiliation: Department of Pathology, University of Cambridge, UK.
authors.forename: J G
authors.initials: JG
authors.lastname: Langley
authors.forename: H C
authors.initials: HC
authors.lastname: Kariuki
authors.forename: A P
authors.initials: AP
authors.lastname: Hammersley
authors.forename: J H
authors.initials: JH
authors.lastname: Ouma
authors.forename: A E
authors.initials: AE
authors.lastname: Butterworth
authors.forename: D W
authors.initials: DW
authors.lastname: Dunne
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000916:Antibody Diversity; D000947:Antigens, Helminth / Q000276:immunology*; D002241:Carbohydrates / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000096:biosynthesis*; D008875:Middle Aged; D010063:Ovum / Q000276:immunology; D010455:Peptides / Q000276:immunology; D012550:Schistosoma mansoni / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D002241:Carbohydrates; D000939:Epitopes; D007074:Immunoglobulin G; D010455:Peptides