title: MOM22 is a receptor for mitochondrial targeting sequences and cooperates with MOM19.
abstract: Recognition of targeting signals is a crucial step in protein sorting within the cell. So far, only a few components capable of deciphering targeting signals have been identified, and insights into the chemical nature of the interaction between the signals and their receptors are scarce. Using highly purified mitochondrial outer membrane vesicles, we demonstrate that MOM22 and MOM19, components of the protein import complex of the outer membrane, bind preproteins at the mitochondrial surface in a reversible fashion. Interaction specifically and directly occurs with the N-terminal presequence and is abolished after inactivation of either MOM22 or MOM19. Binding is salt sensitive, suggesting that recognition involves electrostatic forces between the positive charges of the presequence and the acidic cytosolic domain of MOM22. MOM19 and MOM22 can be cross-linked with high efficiency. We propose that the two proteins form a complex which functions as the presequence receptor at the mitochondrial surface and facilitates the movement of preproteins into the translocation pore.
authors.affiliation: Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Mayer
authors.forename: F E
authors.initials: FE
authors.lastname: Nargang
authors.forename: W
authors.initials: W
authors.lastname: Neupert
authors.forename: R
authors.initials: R
authors.lastname: Lill
mesh_terms: D000818:Animals; D005656:Fungal Proteins; D006639:Histidine; D007425:Intracellular Membranes / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D026901:Membrane Transport Proteins; D008928:Mitochondria / Q000378:metabolism*; D008961:Models, Structural; D009492:Neurospora crassa / Q000378:metabolism; D011498:Protein Precursors / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D012156:Reticulocytes / Q000378:metabolism; D016324:Sequence Tagged Sites; D013762:Tetrahydrofolate Dehydrogenase / Q000096:biosynthesis / Q000302:isolation & purification
chemical_list: D005656:Fungal Proteins; C066557:MOM19 protein, Neurospora crassa; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D011498:Protein Precursors; D011956:Receptors, Cell Surface; D018160:Receptors, Cytoplasmic and Nuclear; D011993:Recombinant Fusion Proteins; D006639:Histidine; D013762:Tetrahydrofolate Dehydrogenase