title: Quantitative analysis of messenger ribonucleic acid encoding natriuretic peptide receptors in aldosterone-producing adenoma.
abstract: There exist conflicting data regarding the inhibitory effect of atrial natriuretic peptide on aldosterone production from aldosterone-producing adenoma (APA). Natriuretic peptides mediate their actions through natriuretic peptide receptors (NPRs). Whether or not NPRs are present in the tumors remains controversial. To elucidate this paradox, gene expression of NPRs was examined by Northern blot analysis and competitive polymerase chain reaction in tumorous and non-tumorous portions of APA, and in normal adrenal gland from patients with renal cell carcinoma. The results of Northern blot analysis showed the presence of messenger ribonucleic acid (mRNA) of three NPRs in all adrenal tissues, including APA. The proportional expression of NPR gene transcripts in APA was type A (0.6%), type B (18.7%), and type C (80.7%). The levels, but not the proportions, of type C and possibly type B NPR mRNAs were lower in tumorous and non-tumorous portions of APA compared to those in normal adrenal gland (type C 190.2 +/- 24.5 [means +/- SEM, normal adrenal gland] > 168.1 +/- 20.8 [non-tumorous portion] > 112.2 +/- 15.5 [tumorous portion] pg/10 micrograms total RNA, F = 3.82, P < 0.05; type B 45.2 +/- 8.5 [normal adrenal gland] > 30.0 +/- 5.2 [non-tumorous portion] > 25.1 +/- 4.1 [tumorous portion] pg/10 micrograms total RNA, F = 3.03, P = 0.065). The mRNA levels of type C, rather than type A or type B, NPR were correlated with the percentage of zona fasciculata-like cells in APA (r = 0.90, P < 0.05). In conclusion we have demonstrated the presence of mRNA encoding the three NPRs in APA.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: College of Medicine, National Taiwan University, National Taiwan University Hospital, Taipei, Republic of China.
authors.forename: Y M
authors.initials: YM
authors.lastname: Chen
authors.forename: K D
authors.initials: KD
authors.lastname: Wu
authors.forename: K Y
authors.initials: KY
authors.lastname: Hung
authors.forename: Y S
authors.initials: YS
authors.lastname: Pu
authors.forename: B S
authors.initials: BS
authors.lastname: Hsieh
mesh_terms: D000236:Adenoma / Q000378:metabolism* / Q000473:pathology; D000310:Adrenal Gland Neoplasms / Q000378:metabolism* / Q000473:pathology; D000450:Aldosterone / Q000096:biosynthesis* / Q000097:blood; D001483:Base Sequence; D001667:Binding, Competitive; D015152:Blotting, Northern; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D017461:Receptors, Atrial Natriuretic Factor / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; C058930:brain natriuretic peptide receptor; D000450:Aldosterone; D017461:Receptors, Atrial Natriuretic Factor