Expression in vivo of CD45RA, CD45RB and CD44 on T cell receptor-transgenic CD8+ T cells following immunization.
We used mice transgenic for a major histocompatibility complex class I-restricted T cell receptor to study the changes of phenotype in vivo which follow priming by antigen of CD8 T cells. We show that following priming with peptide, CD44 on CD8 T cells is up-regulated. The change of phenotype was relatively stable, as primed CD8 cells isolated from thymectomized mice 6 weeks after priming still expressed increased levels of CD44. CD8 T cells in these mice are still responsive to peptide and could represent long-lived primed cells. No down-regulation in vivo of the CD45RA or CD45RB isoforms was found, indicating that there is a differential regulation of the expression of CD44 and CD45RB by activated CD8 transgenic T cells. These results contradict earlier studies in vitro which showed that CD8 T cells which have been primed earlier belong to the CD45RA- or CD45RB- subset.
Pihlgren
M
M
Ecole Normale Supérieure de Lyon, Laboratoire de Biologie Moléculaire et Cellulaire, France.
Lightstone
L
L
Mamalaki
C
C
Rimon
G
G
Kioussis
D
D
Marvel
J
J
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002352:Carrier Proteins / Q000096:biosynthesis / Q000276:immunology*; D018960:Hyaluronan Receptors; D007114:Immunization; D017493:Leukocyte Common Antigens / Q000096:biosynthesis / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000276:immunology*; D016201:Receptors, Lymphocyte Homing / Q000096:biosynthesis / Q000276:immunology*
D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D011948:Receptors, Antigen, T-Cell; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D017493:Leukocyte Common Antigens