Regional assignment of conserved reference loci anchors unassigned linkage and syntenic groups to ovine chromosomes.
Seven loci that have been previously mapped to human and mouse chromosomes have now been regionally assigned to six sheep chromosomes. Nerve growth factor beta (NGFB), antigen CD3 zeta polypeptide (CD3Z), inhibin beta A (INHBA), estrogen receptor (ESR), rhodopsin (RHO), insulin-like growth factor 2 (IGF2), and myelin basic protein (MBP) were mapped by in situ hybridization to sheep chromosomes 1p24-p21, 1p14-p11, 4q26-q31, 8q25-q27, 19q23-qter, 21q21-qter, and 23q11-q12.3, respectively. ESR, RHO, IGF2, and MBP are the first markers to be assigned to their respective sheep chromosomes. These new data allow the previously unassigned sheep linkage groups H, J, K, and S to be provisionally assigned to chromosomes 21, 19, 4, and 8, respectively. The unassigned sheep syntenic groups U8 and U13 are provisionally assigned to sheep chromosomes 8 and 21, respectively. The new assignments support the emerging picture that there is extensive conservation of human chromosomal segments in the sheep and cattle genomes. The position of another evolutionary breakpoint on human chromosome 1q is suggested.
Ansari
H A
HA
Gene Mapping Unit, Grasslands Research Centre, Palmerston North, New Zealand.
Pearce
P D
PD
Maher
D W
DW
Broad
T E
TE
D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D017252:CD3 Complex / Q000235:genetics; D002417:Cattle; D002874:Chromosome Mapping; D017124:Conserved Sequence; D018076:DNA, Complementary; D008040:Genetic Linkage; D005819:Genetic Markers; D006801:Humans; D017403:In Situ Hybridization; D007265:Inhibins / Q000235:genetics; D007335:Insulin-Like Growth Factor II / Q000235:genetics; D051379:Mice; D004676:Myelin Basic Protein / Q000235:genetics; D009414:Nerve Growth Factors / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics; D012243:Rhodopsin / Q000235:genetics; D012756:Sheep / Q000235:genetics*
D017252:CD3 Complex; D018076:DNA, Complementary; D005819:Genetic Markers; D004676:Myelin Basic Protein; D009414:Nerve Growth Factors; D011960:Receptors, Estrogen; D007265:Inhibins; D007335:Insulin-Like Growth Factor II; D012243:Rhodopsin