The effect of interaction between (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (1S,3R)-ACPD and noradrenaline on cyclic AMP accumulation: different actions in brain slices, primary glial and neuronal cell cultures.
The existence of multiple metabotropic receptors for excitatory amino acids is firmly established by recent cloning experiments. Several subtypes of those receptors are coupled to adenylate cyclase. (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (1S,3R)-ACPD) is a selective agonist for metabotropic glutamate receptors, it influences cyclic AMP accumulation in brain and is able to enhance the effect of other substances which stimulate cyclic AMP accumulation. Here we present data showing that (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid enhanced the action of noradrenaline on cyclic AMP accumulation in rat brain cortical slices (EC50 40 microM). On the contrary, it inhibited the action of noradrenaline in primary glial cell cultures (EC50 50 microM), being without any effect on noradrenaline-induced cyclic AMP accumulation in primary neuronal cell cultures. The differences may reflect stimulation of different types of metabotropic glutamate receptors in various preparations from rat brain. The nature of interaction between noradrenaline and (1S,3R)-ACPD remains to be studied.
Pilc
A
A
Institute of Pharmacology, Polish Academy of Sciences, Kraków.
Legutko
B
B
Frankiewicz
T
T
Czyrak
A
A
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D003515:Cycloleucine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004347:Drug Interactions; D066298:In Vitro Techniques; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009498:Neurotoxins / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists / Q000187:drug effects / Q000378:metabolism
D009498:Neurotoxins; D018094:Receptors, Metabotropic Glutamate; D003515:Cycloleucine; C036361:1-amino-1,3-dicarboxycyclopentane; D000242:Cyclic AMP; D009638:Norepinephrine