Enzyme immunometric assay for L-thyroxine using direct ultraviolet irradiation.
A new enzyme immunometric assay for L-thyroxine using uv irradiation as a cross-linking procedure is described. L-Thyroxine in plasma samples is immunocaptured by a monoclonal anti-L-thyroxine antibody coated on 96-well microtiter plates. After uv irradiation and methanol treatment, the covalently linked L-thyroxine is measured using the same monoclonal anti-L-thyroxine antibody labeled with acetylcholinesterase. A minimal detectable concentration of 4.8 nmol/liter was observed with a coefficient of variation less than 16% in the 20-320 nmol/liter. Specificity of the assay was very satisfying and a good correlation (r = 0.959) was noted for 33 human plasma samples between this assay and a commercial competitive radioimmunoassay.
Etienne
E
E
CEA, Service de Pharmacologie et d'Immunologie, Département de Recherche en Imagerie, Pharmacologie et Physiologie, Gif sur Yvette, France.
Créminon
C
C
Lamourette
P
P
Grassi
J
J
Pradelles
P
P
D000911:Antibodies, Monoclonal; D003429:Cross Reactions; D003432:Cross-Linking Reagents; D000939:Epitopes; D006801:Humans; D007124:Immunoenzyme Techniques; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013974:Thyroxine / Q000097:blood*; D014466:Ultraviolet Rays
D000911:Antibodies, Monoclonal; D003432:Cross-Linking Reagents; D000939:Epitopes; D013974:Thyroxine