Primary structure of a novel neuropeptide isolated from the corpora cardiaca of periodical cicadas having adipokinetic and hypertrehalosemic activities.
A new neuropeptide hormone was isolated from the corpora cardiaca of the periodical cicadas, Magicicada species. Primary structure of the peptide as determined by a combination of automated Edman degradation after enzymatic deblocking with pyroglutamate aminopeptidase and mass spectrometry is: pGlu-Val-Asn-Phe-Ser-Pro-Ser-Trp-Gly-Asn-NH2. Synthetic peptide assayed in the green stink bug Nezara viridula caused a 112% increase in hemolymph lipids at a dose of 0.625 pmol, and a 67% increase in hemolymph carbohydrates at a dose of 2.5 pmol. Based on these results we designate this peptide, a first from order Homoptera, as Magicicada species-adipokinetic hormone (Mcsp-AKH).
Raina
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Insect Neurobiology and Hormone Laboratory, USDA, Beltsville, MD 20705, USA.
Pannell
L
L
Kochansky
J
J
Jaffe
H
H
D000595:Amino Acid Sequence; D000818:Animals; D050260:Carbohydrate Metabolism; D005260:Female; D006430:Hemiptera; D007301:Insect Hormones / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology*; D007313:Insecta / Q000737:chemistry*; D050356:Lipid Metabolism; D008297:Male; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology*; D009490:Neurosecretory Systems / Q000737:chemistry*; D009842:Oligopeptides / Q000502:physiology*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives
D007301:Insect Hormones; D009479:Neuropeptides; D009842:Oligopeptides; C044833:adipokinetic hormone; C095969:adipokinetic hormone, Magicicada; C084112:hypertrehalosemic hormone; D011761:Pyrrolidonecarboxylic Acid