title: Expression of CD44 splice variants in human cutaneous melanoma and melanoma cell lines is related to tumor progression and metastatic potential.
abstract: Expression of CD44, particularly of certain splice variants, has been linked to tumor progression and metastasis formation in a number of different animal and human cancers. Because human cutaneous melanoma is among the most aggressive human cancers, we explored expression of CD44 isoforms (CD44v) in lesions of melanocytic tumor progression. In addition, by RT-PCR and FACS analysis we assessed CD44v RNA species and cell surface expression of CD44v in cultured melanocytes isolated from human foreskin and in a panel of 2 non-, 2 sporadically and 2 highly metastatic human melanoma cell lines. We observed that all melanocytic lesions examined showed strong uniform expression of standard CD44 (CD44s) epitopes. We did not detect CD44v6 expression in the melanocytic lesions. However, CD44 isoforms containing v5 or v10 were differentially expressed. V5 was expressed in 16%, 0%, 20%, 67% and 58% of common nevi, atypical nevi, early primary melanomas (< or = 1.5 mm), advanced primary melanomas (> 1.5 mm) and metastases, respectively, and hence was related to tumor progression. In contrast, CD44v10 was expressed in all common nevi, whereas part of the atypical nevi and most primary melanomas and metastases lacked v10. CD44v RNA patterns were closely similar in cultured melanocytes and all melanoma cell lines. Melanocytes expressed high levels of CD44s but no CD44v, whereas all melanoma cell lines expressed CD44v at the surface. Interestingly, expression of v5 was strongly increased in the highly metastatic cell lines. Our results suggest a role for CD44 variant domains, particularly v5 and v10, in human melanocytic tumor progression.
authors.affiliation: Department of Pathology, Academic Medical Center, University of Amsterdam, The Netherlands.
authors.forename: E
authors.initials: E
authors.lastname: Manten-Horst
authors.forename: E H
authors.initials: EH
authors.lastname: Danen
authors.forename: L
authors.initials: L
authors.lastname: Smit
authors.forename: M
authors.initials: M
authors.lastname: Snoek
authors.forename: I C
authors.initials: IC
authors.lastname: Le Poole
authors.forename: G N
authors.initials: GN
authors.lastname: Van Muijen
authors.forename: S T
authors.initials: ST
authors.lastname: Pals
authors.forename: D J
authors.initials: DJ
authors.lastname: Ruiter
mesh_terms: D001483:Base Sequence; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D006801:Humans; D018960:Hyaluronan Receptors; D008544:Melanocytes / Q000737:chemistry; D008545:Melanoma / Q000737:chemistry* / Q000473:pathology / Q000556:secondary; D008969:Molecular Sequence Data; D009506:Nevus / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000235:genetics; D016201:Receptors, Lymphocyte Homing / Q000032:analysis* / Q000235:genetics; D012878:Skin Neoplasms / Q000737:chemistry* / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing