Flow cytometric analysis of c-myc oncoprotein in non-small-cell lung carcinoma: comparison with immunohistochemical results.
c-myc oncoprotein expression was evaluated in 36 non-small-cell lung carcinomas using immunohistochemical and flow cytometric analysis. Formalin fixed and paraffin embedded material was used. The same monoclonal antibody (mouse anti-human c-myc) was employed both for immunohistochemistry and flow cytometry. For the immunohistochemical evaluation we calculated the percentage of stained cells on 200 neoplastic cells. c-myc oncoprotein was measured using flow cytometry by linking the monoclonal with a secondary FITC-conjugated antibody; for each sample 20,000 events were analysed and the percentage of cells positive for green fluorescence was calculated. DNA content was obtained by propidium iodide staining. Results showed a high correlation between immunohistochemical and cytometric data, suggesting that flow cytometry could be used as an alternative to immunohistochemistry in evaluating nuclear antigens. Moreover, using flow cytometry information on DNA content can be obtained simultaneously.
De Santis
L
L
Cattedra di Anatomia ed Istologia Patologica, IRCCS H San Raffaele, Milano, Italy.
Mangili
F
F
Sassi
I
I
Di Rocco
M
M
Rossi
C
C
Cantaboni
A
A
D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology; D018287:Carcinoma, Large Cell / Q000737:chemistry / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000737:chemistry / Q000473:pathology; D005434:Flow Cytometry; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000737:chemistry* / Q000473:pathology; D016271:Proto-Oncogene Proteins c-myc / Q000032:analysis*
D016271:Proto-Oncogene Proteins c-myc