Cyclophilin binding to the human immunodeficiency virus type 1 Gag polyprotein is mimicked by an anti-cyclosporine antibody.
Human immunodeficiency virus type 1 is unique among retroviruses in that infectivity requires specific incorporation into virions of the cellular protein cyclophilin A through interactions with the Gag polyprotein. Here we show that monoclonal antibody B11 1.4, which recognizes a cyclophilin-binding epitope on cyclosporine, detects denatured or native human immunodeficiency virus type 1 capsid. B11 1.4 does not recognize the capsids of other retroviruses, and binding is inhibited by cyclosporine or by cyclophilin A.
Franke
E K
EK
Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Chen
B X
BX
Tatsis
I
I
Diamanduros
A
A
Erlanger
B F
BF
Luban
J
J
D000591:Amino Acid Isomerases / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002213:Capsid / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D018833:Chaperonins / Q000378:metabolism; D016572:Cyclosporine / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D015683:Gene Products, gag / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D007700:Kinetics; D019696:Peptidylprolyl Isomerase; D011485:Protein Binding; D012190:Retroviridae / Q000378:metabolism; D013045:Species Specificity
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D002352:Carrier Proteins; D000939:Epitopes; D015683:Gene Products, gag; D016572:Cyclosporine; D018833:Chaperonins; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase