Specific cleavage of two target sequences of the tobacco mosaic virus RNA by a di-component ribozyme.
Two di-component ribozymes RZ34 and RZ13, the simplest forms of multi-component ribozymes, by ligating mono-ribozymes RZ3 and RZ4 and RZ1 and RZ3 were constructed, respectively. The in vitro results indicate that each di-component ribozyme is able to cleave two different TMV transcripts, with the specificity and efficiency similar to their mono-ribozyme counterparts. Possible effects on the specificity and efficiency by temperature and the positions of the component-ribozymes in these di-component ribozymes were also studied.
Li
J
J
National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, Chinese Academy of Sciences.
Jin
H
H
Zhang
Q
Q
Song
R
R
Xu
Z
Z
D001483:Base Sequence; D008969:Molecular Sequence Data; D016337:RNA, Catalytic / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature; D014027:Tobacco Mosaic Virus / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
D016337:RNA, Catalytic; D012367:RNA, Viral