The Drosophila cation channel trpl expressed in insect Sf9 cells is stimulated by agonists of G-protein-coupled receptors.
Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl, which are assumed to be involved in calcium influx, serve as model system, trpl expressed in Sf9 cells showed spontaneous activity. Hormonal stimulations of calcium influx (detected by fura-2) and of an outwardly rectifying current were observed in Sf9 cells coinfected with baculoviruses encoding trpl and various heptahelical receptors for histamine, thrombin, and thromboxane A2, all known to cause phospholipase C-beta activation in mammalian cells. Although the identity of the G-proteins and of possible second messengers involved need to be clarified, it is clear that trpl represents a receptor/G-protein regulated cation channel.
Harteneck
C
C
Institut für Pharmakologie, Freie Universität Berlin, Germany.
Obukhov
A G
AG
Zobel
A
A
Kalkbrenner
F
F
Schultz
G
G
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002118:Calcium / Q000378:metabolism; D002148:Calmodulin-Binding Proteins / Q000378:metabolism*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D019204:GTP-Binding Proteins / Q000378:metabolism*; D007473:Ion Channels / Q000819:agonists / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D017956:Photoreceptor Cells, Invertebrate / Q000187:drug effects / Q000378:metabolism*; D018411:Spodoptera; D050051:Transient Receptor Potential Channels
D002148:Calmodulin-Binding Proteins; D018076:DNA, Complementary; D029721:Drosophila Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D050051:Transient Receptor Potential Channels; C074192:trpl protein, Drosophila; D019204:GTP-Binding Proteins; D002118:Calcium