Mutations in the sequence flanking the microsatellite at the KAP8 locus prevent the amplification of some alleles.
The microsatellite described for the glycine- and tyrosine-rich keratin locus (KAP8) in sheep was used to type nine large three-generation families comprising the AgResearch International Mapping Flock. The apparent non-Mendelian inheritance in these families suggested that some of the microsatellite alleles were not being amplified. Sequence analysis showed that a deletion within one of the published primer sites was the likely cause of the 'null' alleles. By redesigning the primer all alleles could be amplified.
Ede
A J
AJ
Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Crawford
A M
AM
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000662:veterinary; D004276:DNA, Satellite; D005260:Female; D005784:Gene Amplification; D007633:Keratins / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D017384:Sequence Deletion; D012756:Sheep / Q000235:genetics*
D004276:DNA, Satellite; D007633:Keratins