title: Location by fluorescence microscopy of glycosidases and a xylanase in the anaerobic gut fungi Caecomyces communis, Neocallimastix frontalis, and Piromyces rhizinflata.
abstract: beta-D-Glucosidase, beta-D-fucosidase, beta-D-xylosidase, and beta-D-cellobiopyranosidase activities in Caecomyces communis, Neocallimastix frontalis, and Piromyces rhizinflata, located with fluorescent conjugates, occur throughout the whole thallus as from zoospore germination and disappear before sporulation. beta-D-Galactosidase and alpha-L-arabinopyranosidase activities are low or nonexistent. A xylanase, detected by indirect immunofluorescence, was observed at the surface of the vegetative cells, vesicles, or rhizoids. Cross-reactions prove the existence of analogies in structure among the enzymes of these anaerobic gut fungi.
authors.affiliation: Laboratoire de Microbiologie, INRA, Centre de Recherches de Clermont-Ferrand-Theix, Saint-Genès-Champanelle, France.
authors.forename: A
authors.initials: A
authors.lastname: Breton
authors.forename: B
authors.initials: B
authors.lastname: Gaillard-Martinie
authors.forename: C
authors.initials: C
authors.lastname: Gerbi
authors.forename: B
authors.initials: B
authors.lastname: Gomez de Ségura
authors.forename: R
authors.initials: R
authors.lastname: Durand
authors.forename: B
authors.initials: B
authors.lastname: Kherratia
mesh_terms: D000818:Animals; D005656:Fungal Proteins / Q000302:isolation & purification*; D005658:Fungi / Q000201:enzymology*; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D008856:Microscopy, Fluorescence; D012417:Rumen / Q000382:microbiology*; D012756:Sheep; D043325:Xylan Endo-1,3-beta-Xylosidase; D014995:Xylosidases / Q000302:isolation & purification*
chemical_list: D005656:Fungal Proteins; D006026:Glycoside Hydrolases; D014995:Xylosidases; D043325:Xylan Endo-1,3-beta-Xylosidase