title: Inhibitory effect of phosphorylated oligosaccharides prepared from potato starch on the formation of calcium phosphate.
abstract: The inhibitory effect of phosphorylated oligosaccharides, which were prepared from potato starch, on the formation of calcium phosphate in vitro were investigated. Phosphorylated oligosaccharides from potato were fractionated by ion-exchange chromatography into two fractions, PO-1 and PO-2. Fraction PO-1 was composed of maltotriose, maltotetraose, and maltopentaose to which one phosphate group was attached. Fraction PO-2 was predominantly composed of maltopentaose and maltohexaose to which at least two phosphate groups were attached. The average degree of polymerization of dephosphorylated PO-1 and PO-2 was evaluated to be 4.02 and 5.82, respectively. Fraction PO-2 was the main component having an inhibitory effect on calcium phosphate formation. In addition, among the phosphorylated monosaccharides, glucose-1,6-diphosphate and fructose-1,6-diphosphate were more effective inhibitors of the formation of calcium phosphate than glucose-6-phosphate and fructose-6-phosphate. These results suggest that the strength of the inhibitory effect might depend on the number of phosphate groups attached to each sugar molecule.
authors.affiliation: Biochemical Research Laboratory, Ezaki Glico Co., Ltd., Osaka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kamasaka
authors.forename: M
authors.initials: M
authors.lastname: Uchida
authors.forename: K
authors.initials: K
authors.lastname: Kusaka
authors.forename: K
authors.initials: K
authors.lastname: Yoshikawa
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: S
authors.initials: S
authors.lastname: Okada
authors.forename: T
authors.initials: T
authors.lastname: Ichikawa
mesh_terms: D002118:Calcium / Q000378:metabolism; D002130:Calcium Phosphates / Q000378:metabolism*; D002240:Carbohydrate Sequence; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000494:pharmacology*; D010766:Phosphorylation; D011198:Solanum tuberosum; D013213:Starch
chemical_list: D002130:Calcium Phosphates; D009844:Oligosaccharides; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; D013213:Starch; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous; D002118:Calcium