title: Analytical evaluation of a newly developed ELISA mass assay for pancreatic amylase.
abstract: OBJECTIVE
  To evaluate a newly developed ELISA mass assay for Pancreatic Amylase.
  
  
  METHODS
  The serum levels of pancreatic amylase were measured by an in-house developed ELISA assay on microtitre plate and compared with two activity assays: immunoinhibition and electrophoresis.
  
  
  RESULTS
  The proposed method was accurate and precise, as indicated by a recovery of 102% and coefficient of variation of less than 5% for within-run and less than 10% for between-run assay. The proposed assay showed good correlation with the activity assays (r = 0.98). The mass concentrations were three times higher than the activities. The relative values (mass or activity units/upper reference limits), however, were concordant in controls and in pancreatic and non-pancreatic conditions.
  
  
  CONCLUSIONS
  Our results indicate that the activity measurements and mass concentrations of pancreatic amylase in serum are comparable and interchangeable after adjusting for the reference range.
authors.affiliation: Department of Laboratory Medicine, University Hospital (University of Western Ontario), London, Canada.
authors.forename: V
authors.initials: V
authors.lastname: Bhayana
authors.forename: S
authors.initials: S
authors.lastname: Cohoe
authors.forename: M L
authors.initials: ML
authors.lastname: Ross
mesh_terms: D000681:Amylases / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D007527:Isoenzymes; D000681:Amylases