Simultaneous determination of betaine and N,N-dimethylglycine in urine.
A method is described for the determination of betaine and its metabolite N,N-dimethylglycine in human urine. The method involves a deamination step and a solid-phase extraction to isolate both substances from urine followed by high performance liquid chromatography (HPLC) separation on a 5-microns SCX column with UV absorbance detection. Limit of detection is about 0.9 micrograms for both substances. Recoveries were > 85%. The method appears to be suitable for monitoring betaine and its metabolite in urine of patients undergoing therapy with betaine.
Laryea
M D
MD
University-Children's Hospital, Düsseldorf, Germany.
Zass
R
R
Ritgen
J
J
Wendel
U
U
D001622:Betaine / Q000302:isolation & purification / Q000627:therapeutic use / Q000652:urine*; D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006712:Homocystinuria / Q000188:drug therapy / Q000652:urine; D006801:Humans; D015203:Reproducibility of Results; D012521:Sarcosine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000652:urine
D001622:Betaine; C025138:dimethylglycine; D012521:Sarcosine