title: Allogeneic lymphocyte chimerism after clinical lung transplantation.
abstract: Donor lungs contain large amounts of passenger leukocytes which are transferred to the recipient by organ transplantation. In this study we have analysed the fate of these cells and have studied the populations of donor leucocytes detectable in the blood circulation of ten lung transplanted patients during the first postoperative weeks. To this aim we have applied immunocytological as well as flow cytometric analyses using monoclonal antibodies against polymorphic HLA class I antigens that differed between donor and recipient as well as antibodies against cell differentiation markers. The results demonstrate that donor cells can be detected in the circulation of all lung transplanted patients but there is a considerable interindividual variability between 0.9% and 17.5% (mean 5.1%) on postoperative day 3. Cells were usually detectable for 2-4 weeks and had disappeared in all patients after 1 month. The circulating donor cells consisted exclusively of lymphocytes. T cells were the predominant population, most of which seemed to be CD45R0+, but B and NK (natural killer) cells were also present. Probably due to the small numbers of patients studied no correlation between clinical parameters and the extent of donor lymphocyte persistence; there were no clinical graft-versus-host reactions. The findings demonstrate the regular existence of a transient (macro)chimerism due to passenger lymphocytes in the early phase after lung transplantation. The immunological function and the relation between this phenomenon and the long-term microchimerism which frequently develops after solid organ transplantation remain unclear.
authors.affiliation: Klinik für Abdominal- und Transplantationschirurgie, Medizinische Hochschule Hannover, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Richter
authors.forename: G
authors.initials: G
authors.lastname: Raddatz
authors.forename: T
authors.initials: T
authors.lastname: Graeter
authors.forename: H J
authors.initials: HJ
authors.lastname: Schäfers
authors.forename: H J
authors.initials: HJ
authors.lastname: Schlitt
mesh_terms: D000328:Adult; D000943:Antigens, Differentiation / Q000097:blood; D006680:HLA Antigens / Q000097:blood; D016027:Heart Transplantation; D006801:Humans; D016040:Lung Transplantation; D016131:Lymphocyte Subsets / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D018183:Transplantation Chimera / Q000276:immunology*
chemical_list: D000943:Antigens, Differentiation; D006680:HLA Antigens