Plasma endotoxin concentration and endotoxin binding capacity of plasma acute phase proteins in cirrhotics with variceal bleeding: an analysis by new methods.
Plasma endotoxin levels in 12 cirrhotics with bleeding from oesophageal varices and 50 cirrhotics without bleeding were measured by the chromogenic assay after the pretreatment of sample by perchloric acid (HClO4) and triethylamine. Endotoxin in cirrhotics with bleeding from varices was significantly higher than those without bleeding. In patients with bleeding, endotoxin increased for 3 days after the bleeding, first in the supernatant fraction and then in the precipitate fraction by HClO4 treatment. Peak plasma alpha 1-acid glycoprotein and haptoglobin were observed 3 days after the bleeding. Alpha 1-antitrypsin gradually increased for 14 days. Transferrin did not markedly change. The endotoxin-binding capacity of transferrin and alpha 1-acid glycoprotein increased immediately after bleeding and thereafter decreased, but that of alpha 1-antitrypsin tended to increase in the recovery period. In summary, the plasma endotoxin concentration and endotoxin-binding capacity of alpha 1-acid glycoprotein and transferrin were shown to have increased after bleeding from varices by this new method. There may be a close relationship between endotoxaemia and acute phase reaction in this situation.
Fukui
H
H
Third Department of Internal Medicine, Nara Medical University, Kashihara Japan.
Matsumoto
M
M
Tsujita
S
S
Takaya
A
A
Kojima
H
H
Matsumura
M
M
Tsujii
T
T
D000209:Acute-Phase Proteins / Q000378:metabolism*; D002863:Chromogenic Compounds; D004731:Endotoxins / Q000097:blood* / Q000378:metabolism; D004932:Esophageal and Gastric Varices / Q000097:blood* / Q000209:etiology; D006471:Gastrointestinal Hemorrhage / Q000097:blood* / Q000209:etiology; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood* / Q000150:complications; D013997:Time Factors
D000209:Acute-Phase Proteins; D002863:Chromogenic Compounds; D004731:Endotoxins