title: Carbon fibre micro-electrodes for concomitant in vivo electrophysiological and voltammetric measurements: no reciprocal influences.
abstract: Differential pulse voltammetry and more recently cyclic voltammetry have been successfully used to monitor basal levels of endogenous chemicals by means of treated carbon fibre microbiosensors inserted in specific brain regions. In this study, feasibility of concomitant in vivo recordings of stable electrophysiological signals and basal ascorbate, catecholaminergic and indolaminergic voltammetric peaks at the same cerebral site by means of a single electrically treated carbon fibre micro electrode (microbiosensor) is presented. The results indicate that these two independent techniques can be combined in vivo at a single electrode, and that voltammetric measurements of unstimulated levels of extracellular compounds do not alter concomitant basal cell firing for a period long enough (more than 6 h) to allow pharmacological manipulations.
authors.affiliation: Glaxo Research Laboratories, Department of Pharmacology, Verona, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Crespi
authors.forename: T
authors.initials: T
authors.lastname: England
authors.forename: E
authors.initials: E
authors.lastname: Ratti
authors.forename: D G
authors.initials: DG
authors.lastname: Trist
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism; D015374:Biosensing Techniques; D001921:Brain / Q000378:metabolism / Q000502:physiology*; D002244:Carbon; D000077482:Carbon Fiber; D002396:Catechols / Q000378:metabolism; D004558:Electric Stimulation; D004567:Electrodes, Implanted; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods*; D005240:Feasibility Studies; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007211:Indoles / Q000378:metabolism; D008839:Microelectrodes; D009714:Nucleus Accumbens / Q000378:metabolism; D051381:Rats
chemical_list: D000077482:Carbon Fiber; D002396:Catechols; D007211:Indoles; D015102:3,4-Dihydroxyphenylacetic Acid; D006897:Hydroxyindoleacetic Acid; D002244:Carbon; C030374:indole; C034221:catechol; D001205:Ascorbic Acid