title: The stable bacteriocin release protein signal peptide, expressed as a separate entity, functions in the release of cloacin DF13.
abstract: The pCloDF13 encoded bacteriocin release protein (BRP) plays a role in the release of the bacteriocin cloacin DF13. The BRP signal peptide is stable after cleavage, and accumulates in the cytoplasmic membrane. A BRP which is correctly targeted by the unstable murecin lipoprotein signal peptide (Lpp-BRP) is not capable of inducing the release of cloacin DF13. To investigate the role of the stable BRP signal peptide in the release of cloacin DF13, the stable BRP signal peptide and the Lpp-BRP were expressed in trans in cells also producing cloacin DF13. Expression and release experiments indicate that the stable signal peptide can complement the Lpp-BRP in the release of cloacin DF 13.
authors.affiliation: Department of Molecular Microbiology, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: F J
authors.initials: FJ
authors.lastname: van der Wal
authors.forename: C M
authors.initials: CM
authors.lastname: ten Hagen
authors.forename: B
authors.initials: B
authors.lastname: Oudega
authors.forename: J
authors.initials: J
authors.lastname: Luirink
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002989:Cloacin / Q000096:biosynthesis*; D004274:DNA, Recombinant / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins; D015870:Gene Expression; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D010457:Peptidoglycan / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004274:DNA, Recombinant; D029968:Escherichia coli Proteins; D010457:Peptidoglycan; D021382:Protein Sorting Signals; C052368:cloacin DF13-encoded bacteriocin release protein, E coli; D007544:Isopropyl Thiogalactoside; D002989:Cloacin