title: Differential phosphorylation of a 57-KDa protein tyrosine kinase during egg activation.
abstract: Fertilization results in activation of many protein kinases which function during egg activation. We have used metabolic labelling and immunoprecipitation to study changes in the phosphorylation state of a 57-KDa src-family protein tyrosine kinase during fertilization of the sea urchin egg. The kinase was phosphorylated on serine at all periods studied but it was also phosphorylated transiently on tyrosine at 5 minutes post insemination and then on threonine at 90 minutes after fertilization. These data indicate that the 57-KDa PTK may be under complex regulatory control during the first cell cycle.
authors.affiliation: Dept. of Anatomy & Cell Biology, Univ. of Kansas Medical Ctr., Kansas City.
authors.forename: W H
authors.initials: WH
authors.lastname: Kinsey
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D002453:Cell Cycle / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005306:Fertilization / Q000502:physiology*; D016391:Genes, src; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009865:Oocytes / Q000502:physiology*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D010710:Phosphates / Q000378:metabolism*; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis; D010769:Phosphothreonine / Q000032:analysis; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D011519:Proto-Oncogenes; D012617:Sea Urchins; D013094:Spermatozoa / Q000502:physiology; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D000906:Antibodies; D010455:Peptides; D010710:Phosphates; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D010769:Phosphothreonine; D010768:Phosphoserine; D019000:Phosphotyrosine; D014443:Tyrosine; D011505:Protein-Tyrosine Kinases