Platelets in ulcerative colitis and Crohn's disease express functional interleukin-1 and interleukin-8 receptors.
Tissue and plasma concentrations of several cytokines are increased in patients with inflammatory bowel disease (IBD). Platelets play an important role in inflammation and circulate in an activated state in patients with IBD. This study assesses the expression of IL-8 and IL-1 receptors on the surface of platelets from patients with IBD using phycoerythrin (PE)-labelled recombinant human rhIL-1 beta and rhIL-8 and flow cytometry. The percentage IL-1R expressing platelets (median and interquartile range IQR) in the IBD group was 8.7% (5.5-18.2) compared to 4.2% (2.3-6.1) in controls (P = 0.02). The percentage IL-8R expressing platelets in the IBD group was 22.5% (16.5-27.9) and 9.2% (4.3-9.6) in controls (P < 0.001). Furthermore, platelet IL-1R expression in patients with IBD was inversely related to the total daily dose of steroids (r = 0.71, P < 0.01 linear regression analysis). Finally, platelet rich plasma from healthy controls was stimulated with rhIL-1 beta and rhIL-8 and assessed for activation dependent expression of platelet aGPIIb/IIIa and CD62 (p-selectin, GMP-140). IL-1 beta and IL-8 in vitro significantly and specifically activated the platelets. The surface membrane of platelets is able to express functional IL-1R and IL-8R, the expression of which is significantly increased in IBD. Interleukin-1 beta and IL-8 modulate platelet activation in vitro indicating a target role for platelet function in inflammation.
Schaufelberger
H D
HD
Department of Gastroenterology and Nutrition, Central Middlesex Hospital, London, UK.
Uhr
M R
MR
McGuckin
C
C
Logan
R P
RP
Misiewicz
J J
JJ
Gordon-Smith
E C
EC
Beglinger
C
C
D000293:Adolescent; D000328:Adult; D001792:Blood Platelets / Q000378:metabolism*; D003093:Colitis, Ulcerative / Q000097:blood*; D003424:Crohn Disease / Q000097:blood*; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D016209:Interleukin-8 / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D019007:P-Selectin; D015539:Platelet Activation / Q000187:drug effects; D010980:Platelet Membrane Glycoproteins / Q000187:drug effects / Q000378:metabolism; D018123:Receptors, Interleukin / Q000378:metabolism*; D017472:Receptors, Interleukin-1 / Q000378:metabolism*; D023062:Receptors, Interleukin-8A
D007375:Interleukin-1; D016209:Interleukin-8; D019007:P-Selectin; D010980:Platelet Membrane Glycoproteins; D018123:Receptors, Interleukin; D017472:Receptors, Interleukin-1; D023062:Receptors, Interleukin-8A