title: Analyses of mAb reactive with porcine CD8.
abstract: Among all mAb submitted to the first porcine CD workshop, based on FCM analyses six mAb could be identified to recognize the porcine CD8 analogue (workshop Nos. 004, 051, 052, 053, 108 and 109). In immunoprecipitation studies three mAb (Nos. 004, 108 and 109) recognized an antigen with an apparent molecular mass of about 35 kDa under reducing conditions and about 70 kDa under non-reducing conditions. The molecular masses of the antigens recognized by the three other mAb (Nos. 051, 052 and 053) are still unknown. Epitope analyses performed by blocking experiments led to the determination of two CD8 epitopes: CD8a and CD8b. CD8a is recognized by mAb Nos. 004, 051 and 052, and CD8b by Nos. 053, 108 and 109.
authors.affiliation: Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Saalmüller
authors.forename: B
authors.initials: B
authors.lastname: Aasted
authors.forename: A
authors.initials: A
authors.lastname: Canals
authors.forename: J
authors.initials: J
authors.lastname: Dominguez
authors.forename: T
authors.initials: T
authors.lastname: Goldman
authors.forename: J K
authors.initials: JK
authors.lastname: Lunney
authors.forename: S
authors.initials: S
authors.lastname: Maurer
authors.forename: M D
authors.initials: MD
authors.lastname: Pescovitz
authors.forename: R
authors.initials: R
authors.lastname: Pospisil
authors.forename: H
authors.initials: H
authors.lastname: Salmon
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D000918:Antibody Specificity / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry / Q000662:veterinary; D008970:Molecular Weight; D013552:Swine / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D016827:CD8 Antigens; D000939:Epitopes