Primary structure of a cytotoxin-like basic protein from Naja naja naja (Indian cobra) venom.
The complete amino acid sequence of a cytotoxin-like basic protein (CLBP) from the venom of Naja naja naja (Indian Cobra) was determined by manual degradation using a 4-dimethylaminoazobenzene-4'-isothiocyanate double-coupling method. Peptide fragments obtained by chemical cleavage with cyanogen bromide and enzymic cleavages with trypsin and Staphylococcus aureus proteases for sequence analysis were purified by reversed-phase chromatography. The total number of amino acid residues was 61, with leucine as the C-terminal residue.
Babu
A S
AS
Department of Studies in Biochemistry, University of Mysore, Manasagangotri, India.
Puri
K D
KD
Gowda
T V
TV
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002845:Chromatography; D003603:Cytotoxins / Q000737:chemistry*; D004546:Elapid Venoms / Q000737:chemistry*; D010450:Endopeptidases / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
D000596:Amino Acids; D003603:Cytotoxins; D004546:Elapid Venoms; D010446:Peptide Fragments; D011506:Proteins; D010450:Endopeptidases