Novel assay for the influenza virus M2 channel activity.
The influenza A M2 ion channel was expressed and activity characterized in Xenopus oocytes. Based on the activation properties of the channels, a high throughput, non-electrophysiological screening assay was developed in order to identify novel inhibitors of the channel. This will facilitate discovery of novel agents to treat influenza viral infections.
Giffin
K
K
Department of Protein Biochemistry, Searle Research and Development, St. Louis, MO.
Rader
R K
RK
Marino
M H
MH
Forgey
R W
RW
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006863:Hydrogen-Ion Concentration; D007473:Ion Channels / Q000032:analysis*; D008564:Membrane Potentials; D009865:Oocytes; D011994:Recombinant Proteins / Q000032:analysis; D014763:Viral Matrix Proteins / Q000032:analysis* / Q000235:genetics; D014982:Xenopus laevis; D000086082:Viroporin Proteins
D007473:Ion Channels; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins; C031540:M-protein, influenza virus; C488937:M2 protein, Influenza A virus; D000086082:Viroporin Proteins