Effects of storing initiated 2-hydroxyethyl methacrylate solutions for embedding tissues for light microscopy: some practical implications.
The effects of storing 2-hydroxyethyl methacrylate (HEMA) solutions for embedding tissues for light microscopy were studied using three commercially available HEMA embedding kits: Technovit 7100, Technovit 8100, and JB-4. These HEMA solutions were examined at various times of storage over a period of one year using a panel of physicochemical techniques including gas chromatography, titration, viscosimetry, determination of the maximum polymerization temperature and the time required to reach the maximum temperature, and detection of degradation products of HEMA monomers by histochemical procedures. The quality of the resin blocks was examined by the observation of mini-folds in sections. Data obtained from these tests showed that the release of by-products as a result of the degradation of the HEMA monomer during storage of HEMA solutions does not occur. Development of cross-linking agents by transesterification of HEMA monomer was not detected either. Gradual decrease of the inhibitor concentration during storage proved to be the main cause of the reduction of shelf-life of HEMA solutions. Inconsistent tissue infiltration after storage may be due to decreased rates of tissue penetration as a result of HEMA chain lengthening. Guidelines for safe and economical handling of HEMA mixtures are given.
Gerrits
P O
PO
Department of Anatomy and Embryology, University of Groningen, The Netherlands.
Eppinger
B
B
D008689:Methacrylates; D008853:Microscopy / Q000379:methods; D013696:Temperature; D016610:Tissue Embedding / Q000379:methods*
D008689:Methacrylates; C005044:hydroxyethyl methacrylate