Prognostic value of CD44 splice variant expression in ovarian cancer.
In 44 ovarian cancers, CD44 variant (CD44v) expression was investigated immunohistochemically using a variant-specific polyclonal antibody. Patients with CD44v-positive carcinomas had a significantly shorter disease-free survival than patients with CD44v-negative tumors. Overall survival was also significantly reduced for stages III and IV of the International Federation of Gynecology and Obstetrics. Furthermore, a highly significant inverse correlation was observed between CD44v expression and preoperative platelet count. Urinary neopterin concentration, a marker of cell-mediated immunostimulation, did not differ between CD44v-positive and -negative ovarian cancer patients. Moreover, in seven ovarian carcinoma cell lines, modulation of CD44v expression was analyzed by living cell radioimmunoassay. Interferon-alpha, interferon-gamma, tumor necrosis factor, transforming growth factor-beta, all-trans retinoic acid and cisplatin did not affect CD44v expression.
Uhl-Steidl
M
M
Department of Obstetrics and Gynecology, Innsbruck University Hospital, Austria.
Müller-Holzner
E
E
Zeimet
A G
AG
Adolf
G R
GR
Daxenbichler
G
G
Marth
C
C
Dapunt
O
O
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001708:Biopterins / Q000031:analogs & derivatives / Q000652:urine; D018394:CA-125 Antigen / Q000097:blood; D002277:Carcinoma / Q000276:immunology* / Q000652:urine; D002352:Carrier Proteins / Q000032:analysis*; D005260:Female; D005629:Frozen Sections; D015870:Gene Expression; D006801:Humans; D018960:Hyaluronan Receptors; D008875:Middle Aged; D019798:Neopterin; D010051:Ovarian Neoplasms / Q000276:immunology* / Q000652:urine; D010976:Platelet Count; D011379:Prognosis; D011956:Receptors, Cell Surface / Q000032:analysis*; D016201:Receptors, Lymphocyte Homing / Q000032:analysis*; D018709:Statistics, Nonparametric; D016019:Survival Analysis; D014407:Tumor Cells, Cultured
D018394:CA-125 Antigen; D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D001708:Biopterins; D019798:Neopterin