title: Cloning and characterization of pentylenetetrazol-related cDNA, PTZ-17.
abstract: cDNAs related to pentylenetetrazol-induced bursting activity in neurons were screened by a differential hybridization method using normal and pentylenetetrazol-treated primary cultured neurons from the cerebral cortex of mice. Twenty clones of candidate cDNA with expression increased or decreased by treatment with pentylenetetrazol were obtained. One of them, PTZ-17, was sequenced. Injection of PTZ-17 derived RNA into Xenopus oocytes showed a large calcium inward current with extracellular application of pentylenetetrazol.
authors.affiliation: Department of Physiology, Kanagawa Dental College, Yokosuka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kajiwara
authors.forename: E
authors.initials: E
authors.lastname: Sugaya
authors.forename: M
authors.initials: M
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Katsuki
authors.forename: H
authors.initials: H
authors.lastname: Nagasawa
authors.forename: N
authors.initials: N
authors.lastname: Yuyama
authors.forename: T
authors.initials: T
authors.lastname: Tsuda
authors.forename: A
authors.initials: A
authors.lastname: Sugaya
authors.forename: M
authors.initials: M
authors.lastname: Motoki
authors.forename: T
authors.initials: T
authors.lastname: Ookura
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects*; D010433:Pentylenetetrazole / Q000378:metabolism*; D012313:RNA / Q000008:administration & dosage / Q000187:drug effects; D014981:Xenopus
chemical_list: D018076:DNA, Complementary; D012313:RNA; D002118:Calcium; D010433:Pentylenetetrazole