Further characterization of the 5'-flanking region of the rat lactase-phlorizin hydrolase gene.
The lactase-phlorizin hydrolase gene is widely used as a marker of intestinal differentiation. Recent evidence demonstrating that transcription plays a major role in the regulation of this gene suggests that study of the 5'-flanking region will allow an understanding of how the expression of this gene is controlled. However, sequence, restriction, and primer extension analysis of a rat genomic clone has revealed that previously published data are incomplete. In the present study, we used a directed sequencing strategy to carefully analyze this region. Our expanded analysis of the 5'-flanking region of the lactase-phlorizin hydrolase gene should facilitate future studies of its structure and function.
Verhave
M
M
Division of Pediatric Gastroenterology and Nutrition, New England Medical Center, Tufts University School of Medicine, Boston, MA.
Krasinski
S D
SD
Maas
S M
SM
Smiers
F J
FJ
Mishra
K
K
Breeuwsma
N G
NG
Naim
H Y
HY
Grand
R J
RJ
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005809:Genes, Regulator; D015698:Genomic Library; D007583:Jejunum / Q000201:enzymology*; D010694:Lactase-Phlorizin Hydrolase / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics; D051381:Rats / Q000235:genetics*; D015183:Restriction Mapping; D016385:TATA Box; D014158:Transcription, Genetic
D017931:DNA Primers; D012313:RNA; D010694:Lactase-Phlorizin Hydrolase