title: Streptococcal glucan-binding lectins do not recognize methylated alpha-1,6 glucans.
abstract: The glucan-binding lectin (GBL) of Streptococcus sobrinus is cell associated, enabling the bacteria to be aggregated by alpha-1,6 glucans. Glucans, such as amylose, pullulan, laminarin and nigeran, have no affinity for the lectin. High molecular weight alpha-1,6 glucans (dextrans) readily aggregate the bacteria, whereas low molecular weight glucans inhibit the aggregation brought about by the high molecular weight species. Methylated glucan T-2000 (an alpha-1,6 glucan with an average molecular weight of 2 x 10(6) Da) aggregated the bacteria very poorly when the extent of methylation (DS, or degree of substitution) was high, and less poorly when the DS was low. Similarly, methylated low molecular weight alpha-1,6 glucan was a poor inhibitor of aggregation induced by the high molecular weight glucan T-2000. Because the methylation occurred primarily on the hydroxyl of C-2, it is suggested that the hydroxyl is needed for formation of the lectin-glucan complex. It appears that the GBL is not only stereospecific in interaction with glucans, but also regio-specific, interacting only with the underivatized alpha-1,6-glucan.
authors.affiliation: Department of Microbiology, University of Louisville, KY 40292, USA.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Singh
authors.forename: K G
authors.initials: KG
authors.lastname: Taylor
authors.forename: R J
authors.initials: RJ
authors.lastname: Doyle
mesh_terms: D001422:Bacterial Adhesion; D001426:Bacterial Proteins; D003911:Dextrans / Q000737:chemistry / Q000378:metabolism*; D005936:Glucans / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D037102:Lectins / Q000737:chemistry / Q000378:metabolism*; D008745:Methylation; D013291:Streptococcus / Q000502:physiology*; D016986:Streptococcus sobrinus / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D003911:Dextrans; D005936:Glucans; D037102:Lectins; C041999:alpha-1,6-dextran; C082429:glucan-binding lectin, Streptococcus sobrinus