Antiangiogenic substance(s) in a tumor cell line with low metastatic potential originating from the BALB/c mouse liver.
Two cell lines were established from liver cells (BALB/c mouse) exposed to benzo(a)pyrene: one was highly metastatic (G-5) and the other poorly metastatic (G-1) to the lung when subcutaneously implanted. However, there was no difference in lung colonization between G-1 and G-5 cells when they were intravenously injected. When G-1 cells were subcutaneously inoculated on one side of the back of mice followed by a challenge on the other side with G-5 cells 10 days later, the growth of the latter tumor was inhibited and the number of metastatic nodules in the lung was reduced. The functional vascular volume of G-1 tumor was less than the G-5 one. In mice bearing G-1 tumors, the neovascularization of intradermally inoculated G-5 cells was reduced. The conditioned medium from G-1 culture contained an inhibitory activity on the growth of endothelial cells from calf pulmonary artery. The inhibitory substance(s) was heat-stable, trichloroacetic acid-soluble, nondialyzable and resistant to various proteinases. The present results imply that G-1 cells produce an antiangiogenic substance(s), probably a polysaccharide(s), which inhibits the angiogenesis required for growth and metastasis of the G-5 tumor.
Tanigaki
Y
Y
Department of Cell Biology, Center for Adult Diseases, Osaka, Japan.
Saeki
Y
Y
Matsuhisa
T
T
Ishiguro
S
S
Mori
Y
Y
Tanaka
H
H
Akedo
H
H
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D002417:Cattle; D017690:Cell Transplantation; D005591:Chemical Fractionation; D002845:Chromatography; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D004622:Embryo, Mammalian; D007271:Injections, Intradermal; D007279:Injections, Subcutaneous; D008099:Liver / Q000098:blood supply / Q000187:drug effects; D008113:Liver Neoplasms / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008168:Lung / Q000098:blood supply*; D008175:Lung Neoplasms / Q000556:secondary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008853:Microscopy; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D011134:Polysaccharides; D001564:Benzo(a)pyrene