Serotonergic activity in rat cerebral arteries depends on dorsal raphe nucleus but not on median raphe nucleus.
The effect of Chlorimipramine and Muscimol on serotonergic activity in rat cerebral arteries and in dorsal and median raphe nuclei were used to study the presence of a serotonergic innervation in the cerebral blood vessels functionally dependent on the brainstem nuclei activity. Serotonergic activity was appraised in rat cerebral arteries from 5-hydroxyindoleacetic acid (5-HIAA) disappearance rate or 5-hydroxytryptophan (5-HTP) accumulation after inhibiting monoamine oxidase (MAO) or aromatic amino acids decarboxylase, respectively. In dorsal and median raphe nuclei the decay with time of 5-HIAA after MAO inhibition was used to estimate serotonergic activity. Chlorimipramine significantly reduced serotonergic activity in cerebral blood vessels and in both raphe nuclei. 5-HIAA basal levels in these blood vessels were not altered by treatment with the drug. Muscimol evoked only a decrease in the serotonergic activity of the median raphe nucleus. These results suggest that rat cerebral arteries receive serotonergic fibers functionally active arising mainly from dorsal raphe nucleus.
Moreno
M J
MJ
Departamento de Fisiología, Universidad Autónoma de Madrid, Spain.
López de Pablo
A L
AL
Conde
M V
MV
Fraile
M L
ML
Marco
E J
EJ
D006916:5-Hydroxytryptophan / Q000378:metabolism; D000818:Animals; D002536:Cerebral Arteries / Q000294:innervation*; D002997:Clomipramine / Q000494:pharmacology; D006897:Hydroxyindoleacetic Acid / Q000097:blood; D008297:Male; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D009118:Muscimol / Q000494:pharmacology; D010293:Pargyline / Q000494:pharmacology; D011903:Raphe Nuclei / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000502:physiology*
D008996:Monoamine Oxidase Inhibitors; D009118:Muscimol; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D010293:Pargyline; D006916:5-Hydroxytryptophan; D002997:Clomipramine