Reinforced cytotoxicity of lymphokine-activated killer cells toward glioma cells by transfection of the killer cells with the gamma-interferon gene.
Lymphokine-activated killer (LAK) cells generated from peripheral blood lymphocytes incubated with recombinant interleukin-2 were transfected with the human gamma-interferon (HuIFN-gamma) gene by means of liposomes having a positive charge on their surface. The cells secreted significant amounts of HuIFN-gamma (reaching more than 5 U/ml) into the culture medium. The HuIFN-gamma produced by the cells induced intercellular adhesion molecule-1 (ICAM-1) and enhanced the expression of Fas antigen on the surface of human glioma cells. Also, LAK cells transfected with HuIFN-gamma gene exhibited reinforcement of cytotoxicity toward human glioma cell lines (U251-MG and SK-MG-1). Furthermore, the reinforcement was significantly quenched by anti-ICAM-1 and/or anti-TNF-alpha monoclonal antibody.
Mizuno
M
M
Department of Neurosurgery, Nagoya University School of Medicine.
Yoshida
J
J
Takaoka
T
T
Sugita
K
K
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000954:Antigens, Surface / Q000378:metabolism; D003470:Culture Media; D003602:Cytotoxicity, Immunologic; D004563:Electrochemistry; D005910:Glioma / Q000276:immunology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000276:immunology; D007371:Interferon-gamma / Q000235:genetics* / Q000502:physiology; D007376:Interleukin-2 / Q000494:pharmacology; D008081:Liposomes; D010455:Peptides / Q000276:immunology*; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology; D019014:fas Receptor
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D003470:Culture Media; D007376:Interleukin-2; D008081:Liposomes; D010455:Peptides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor; D018799:Intercellular Adhesion Molecule-1; C041640:poly(lysyl-(leucyl-poly-alanine)); D007371:Interferon-gamma