Computer-assisted mapping of infarcted and viable regions of gross cardiac sections following experimental myocardial infarction.
We have developed a computer-assisted method that creates digital maps of the viable and infarcted regions of tetrazolium-stained gross cardiac sections. Here we describe and test the method, using a canine occlusion-reperfusion infarction model. Quantitative image analysis showed that the method accurately recorded differences between infarcted and viable regions at a spatial resolution of 5-20 pixels per mm2. Microscopic analysis of tissue samples taken from the sections showed that the maps were accurate. In 15 of 15 cases the histology of the samples matched that predicted by the maps. A comparison of infarct area measurements derived from the maps showed that the method was reproducible. The average intraoperator standard deviation was +/- 8% of a slice's infarcted area and the average interoperator standard deviation was +/- 20%. We conclude that the method creates accurate, detailed, and reproducible maps of the infarcted and viable regions of tetrazolium-stained cardiac sections.
Damm
C J
CJ
Department of Medicine, Columbia University, New York, New York 10027, USA.
Bigger
J T
JT
Ursell
P C
PC
Steinberg
J S
JS
Smith
R
R
Lennon
P F
PF
Cook
J R
JR
D000818:Animals; D003199:Computer Systems; D004285:Dogs; D005544:Forecasting; D007089:Image Enhancement; D007091:Image Processing, Computer-Assisted; D007202:Indicators and Reagents; D009203:Myocardial Infarction / Q000473:pathology*; D017202:Myocardial Ischemia / Q000473:pathology; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000473:pathology*; D015588:Observer Variation; D015203:Reproducibility of Results; D012815:Signal Processing, Computer-Assisted; D012984:Software; D013778:Tetrazolium Salts; D014741:Video Recording
D007202:Indicators and Reagents; D013778:Tetrazolium Salts