[Long-term carriers of genomic structures of the influenza virus in the blood leukocytes of children with congenital pathology of central nervous system].
Children with central nervous abnormality were followed up for a long time (180 days). The clinical samples (nasal swabs, blood samples) were investigated for influenza virus antigens or RNA detection by virologic and molecular biological techniques. No viral isolate was found throughout the follow-up. The use of the polymerase chain reaction made it possible to reveal the long-term persistence (for 180 days) of NS- and genes of influenza A (H1N1) viruses in the leukocytes and of HA-gene in the nasal swabs. No NS-gene was found in the nasal swabs. The polymerase chain reaction appears to be more effective for the diagnosis of persistent influenza infection that the conventional techniques--immunofluorescence and enzyme-linked immunosorbent assay (ELISA).
Bannikov
A I
AI
Rodionova
V B
VB
Grinbaum
E B
EB
Biziukina
E G
EG
Popova
T L
TL
Karpova
L S
LS
Sominina
A A
AA
Kiselev
O I
OI
D001483:Base Sequence; D002493:Central Nervous System Diseases / Q000097:blood / Q000151:congenital* / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005500:Follow-Up Studies; D016679:Genome, Viral; D006801:Humans; D007223:Infant; D007962:Leukocytes / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D009975:Orthomyxoviridae / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D013997:Time Factors; D017735:Virus Latency
D004279:DNA, Viral; D012367:RNA, Viral