Induction of calcium-independent nitric oxide synthase activity in cultured cerebellar granule neurons.
Cultured cerebellar granule neurons were assayed for nitric oxide synthase (NOS) activity by measuring the conversion of L-arginine to L-citrulline. Granule neurons expressed constitutive NOS activity which was calcium-dependent. Treatment of neuron cultures for 24 h with the combined stimulation of IFN-gamma plus IL-1 beta, TNF-alpha and LPS induced NOS activity by 87-fold which was calcium-independent. We conclude that cultured cerebellar granule neurons can express both the constitutive and inducible forms of NOS activity.
Sato
I
I
Department of Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Japan.
Kim
Y
Y
Himi
T
T
Murota
S
S
D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis*; D000818:Animals; D001120:Arginine / Q000378:metabolism; D002118:Calcium / Q000502:physiology*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology* / Q000201:enzymology*; D002956:Citrulline / Q000378:metabolism; D016207:Cytokines / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D009474:Neurons / Q000201:enzymology*; D019001:Nitric Oxide Synthase; D051381:Rats; D017208:Rats, Wistar; D012486:Salmonella typhimurium
D016207:Cytokines; D008070:Lipopolysaccharides; D002956:Citrulline; D007371:Interferon-gamma; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D002118:Calcium