The determination of alpha 1-microglobulin by means of an automated latex immunoassay.
Polystyrene (latex) particles coated with human anti-alpha 1 microglobulin antibodies are used in an automated "kinetic" assay for alpha 1-microglobulin in urine. For values below 12 mg/l, there was no significant difference between two kinds of standard, but above 12 mg/l the results depend on the origin of the alpha 1-microglobulin standard. Correlation between values obtained with both standards was good (r2 = 0.968). The method has a between-run imprecision (CV) of 11-16%. Comparison with two commercial enzyme immunoassays gave a bias of -10% to +40%, while two nephelometric methods differed by 0% to 23%, possibly because the standards used in these methods were of different origin. These results indicate the necessity of standardization of the determination of alpha 1-microglobulin. The detection limit of our method was 0.8 mg/l, enabling the application of the method for epidemiological investigations.
Straub
J P
JP
Coronel Laboratory for Occupational and Environmental Health, University of Amsterdam, The Netherlands.
Baard
M A
MA
du Jour
H A
HA
Verplanke
A J
AJ
Herber
R F
RF
D000510:Alpha-Globulins / Q000652:urine*; D000818:Animals; D000906:Antibodies; D001331:Automation; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007124:Immunoenzyme Techniques; D007840:Latex; D008863:Microspheres; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011137:Polystyrenes; D011480:Protease Inhibitors / Q000652:urine*; D011817:Rabbits / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000510:Alpha-Globulins; D000906:Antibodies; D007840:Latex; D011137:Polystyrenes; D011480:Protease Inhibitors; C016001:alpha-1-microglobulin