Inhibition of cleavage by restriction endonucleases due to modifications induced in SV40 DNA by methyl methanesulfonate.
Methyl methanesulfonate (MMS), a direct mutagen, methylates DNA bases and causes distortions in DNA structure. Supercoiled SV40 DNA was treated in vitro with varying concentrations of MMS from 0.001 mM to 10 mM MMS either for 30 min or 3 h and analysed by electrophoresis in 1% neutral and alkaline agarose gels. The electrophoretic mobility (EPM) of native DNA did not change after treatment with the mutagen, while alkaline gels revealed low MW DNA fragments due to single strand breaks at alkali-sensitive sites generated by the action of MMS. By two-dimensional electrophoresis, we find that all three native DNA forms contain alkali-sensitive sites after treatment with MMS. To examine the effect of base modification by MMS on DNA-protein interactions, we have used as probes, restriction endonucleases. These cleave DNA in a sequence-specific manner, and their activity is dependent upon the methylation status of the substrate DNA. We find that cleavage by these restriction endonucleases is inhibited due to methylation by MMS.
Ghaskadbi
S
S
Department of Zoology, University of Poona, Pune, India.
Bharathi
S
S
Modak
S P
SP
D004249:DNA Damage; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004278:DNA, Superhelical / Q000187:drug effects*; D004279:DNA, Viral / Q000737:chemistry* / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel / Q000379:methods; D016679:Genome, Viral; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D008745:Methylation; D013539:Simian virus 40 / Q000235:genetics*; D013997:Time Factors
D004278:DNA, Superhelical; D004279:DNA, Viral; D008741:Methyl Methanesulfonate; D004262:DNA Restriction Enzymes