Thermal stability of mineralized and demineralized dentin: a differential scanning calorimetric study.
The purpose of this study was to determine the difference, if any, in the thermal stability of collagen in mineralized and demineralized dentine compared to that in unmineralized tissues, using differential scanning calorimetry, DSC. Human tooth dentin blocks, about 1 x 1 x 2 mm in size, were used in this study. Some dentin blocks were demineralized using a Plank Rychlo solution; others, using EDTA solution. The mineralized dentin showed an exothermic peak at about 310 degrees C and the combustion of organic materials was completed at about 450 degrees C. For the demineralized dentin, the combustion was completed at higher temperature range and showed a strong exothermic peak at about 470 degrees C. An exotherm at the temperature between 450 degrees C and 470 degrees C was also observed in DSC pattern of native type I collagen from calf skin and rat tail tendon. DSC pattern of rat tail collagen showed a close similarity to that of the demineralized dentin. Statistically, the same heat flow value was obtained both from the mineralized dentin and the demineralized dentin and from the native type I collagen. These findings indicated that the thermal stability of collagen in dentin is lower than collagen in uncalcified connective tissue. It is suggested that in calcified collagen, the apatite crystallites may have intruded into spaces of the crosslinks of intra- and inter-fibrils, and in so doing, destroyed the crosslinks.
Sakae
T
T
Department of Anatomy, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Mishima
H
H
Kozawa
Y
Y
LeGeros
R Z
RZ
D000818:Animals; D001031:Apatites / Q000032:analysis; D002113:Calcification, Physiologic; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D003094:Collagen / Q000032:analysis*; D003238:Connective Tissue / Q000737:chemistry; D003460:Crystallization; D003648:Decalcification Technique; D003804:Dentin / Q000737:chemistry*; D006801:Humans; D051381:Rats; D012867:Skin / Q000737:chemistry; D013710:Tendons / Q000737:chemistry; D013816:Thermodynamics; D014074:Tooth Calcification
D001031:Apatites; D003094:Collagen