title: A novel neuropeptide cellular mechanism in amphibian interrenal steroidogenesis.
abstract: Interrenals of female Rana esculenta were incubated with gonadotropin-releasing hormone (GnRH), 9-ketoreductase inhibitor (palmitic acid), acetyl salicyclic acid, prostaglandin F2 alpha (PGF2 alpha), forskolin, isobutylmethyl xanthine (IBMX), dibutyril cyclic adenosine monophosphate (dbcAMP). Prostaglandin E2 (PGE2), PGF2 alpha, testosterone and 17 beta-estradiol were assessed on the incubation media. In addition, in the same interrenals, 9-ketoreductase and aromatase activities were evaluated. GnRH increased PGF2 alpha, 17 beta-estradiol, 9-ketoreductase and aromatase, and decreased PGE2 and testosterone. PGF2 alpha increased 17 beta-estradiol and aromatase, and decreased testosterone. Palmitic acid counteracted GnRH effects, while forskolin, IBMX and dbcAMP showed the same PGF2 alpha effects. These results suggest that GnRH stimulates 9-ketoreductase enhancing PGF2 alpha which in turn activates aromatase through cAMP mediation in the interrenal of Rana esculenta.
authors.affiliation: Department of Molecular, Cellular and Animal Biology, University of Camerino, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Gobbetti
authors.forename: M
authors.initials: M
authors.lastname: Zerani
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D001141:Aromatase / Q000378:metabolism; D001241:Aspirin / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D015237:Dinoprost / Q000378:metabolism / Q000494:pharmacology; D015232:Dinoprostone / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism; D066298:In Vitro Techniques; D007401:Interrenal Gland / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D008954:Models, Biological; D019308:Palmitic Acid; D010169:Palmitic Acids / Q000494:pharmacology; D011893:Rana esculenta; D013739:Testosterone / Q000378:metabolism*
chemical_list: D010169:Palmitic Acids; D005576:Colforsin; D019308:Palmitic Acid; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D004958:Estradiol; D003994:Bucladesine; D015237:Dinoprost; D006911:Hydroxyprostaglandin Dehydrogenases; C035858:prostaglandin-E2 9-reductase; D001141:Aromatase; D015232:Dinoprostone; D001241:Aspirin; D015056:1-Methyl-3-isobutylxanthine