Direct toxic effect of bleomycin on alveolar type 2 cells.
We studied the in vitro toxicity of bleomycin (BLM) on primary cultures of rat alveolar type 2 cells (T2 cells). It was shown that BLM was directly toxic for T2 cells in a dose- and time-dependent manner. Lung fibroblasts (LF) appear to be more resistant than T2 cells. Modulation of intracellular glutathione concentration was associated with changes in cytotoxicity. Furthermore, the addition of O-phenanthroline to the cellular medium reduced significantly BLM toxicity, suggesting the involvement of intra-cellular ferric ion. We also found that BLM toxicity was associated with a decreased release of phosphatidylcholine by T2 cells, the main component of surfactant. Protective effect of O-phenanthroline and the involvement of glutathione may be an alternative approach to the protection of BLM-induced damage.
Karam
H
H
Service de Pneumologie, Hôpital Saint-Antoine, Paris, France.
Hurbain-Kosmath
I
I
Housset
B
B
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001761:Bleomycin / Q000037:antagonists & inhibitors / Q000633:toxicity*; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D008297:Male; D010713:Phosphatidylcholines / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; D002614:Chelating Agents; D010713:Phosphatidylcholines; D001761:Bleomycin