Systemic production of foreign peptides on the particle surface of tobacco mosaic virus.
By using a new tobacco mosaic virus (TMV) vector [Hamamoto, H., et al. (1993) Bio/Technology 11, 930-932], we have constructed TMV particles which present three different kinds of epitopes, two of them from influenza virus hemagglutinin (HA), and one from human immunodeficiency virus type I (HIV-I) envelope protein, on the surface of the particles. Each of these TMV particles reacted with each anti-peptide antiserum. These results suggest that this TMV vector can be used as an antigen presentation system.
Sugiyama
Y
Y
Biochemistry Laboratory, Kamebo Ltd., Kanagawa, Japan.
Hamamoto
H
H
Takemoto
S
S
Watanabe
Y
Y
Okada
Y
Y
D000595:Amino Acid Sequence; D017951:Antigen Presentation; D001483:Base Sequence; D004274:DNA, Recombinant; D000939:Epitopes / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D005822:Genetic Vectors; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000302:isolation & purification; D015497:HIV-1 / Q000235:genetics; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009975:Orthomyxoviridae / Q000235:genetics; D010446:Peptide Fragments / Q000235:genetics; D010947:Plants, Toxic; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D014026:Nicotiana / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000235:genetics*
D004274:DNA, Recombinant; D000939:Epitopes; D015699:HIV Envelope Protein gp120; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D006389:Hemagglutinins, Viral; D010446:Peptide Fragments; D011994:Recombinant Proteins