Lipid peroxidation products mediate damage of superoxide dismutase.
Membrane lipid peroxidation processes yield products that may react with superoxide dismutase (SOD), one of the key antioxidant enzymes against oxidative stress, resulting in oxidative modifications. We have investigated this possibility and have found that three types of SOD--CuZnSOD, MnSOD, and FeSOD--exposed to autoxidized linolenate in the presence of Fe3+ caused the loss of dismutase activity, fragmentation of peptides, and an increase in protein oxidation. Lysates from E. coli oxyR strains treated with tert-butyl hydroperoxide, which presumably induces lipid peroxidation, resulted in the activation of SOD. The lipid peroxidation-mediated damage to SOD may result in the perturbation of cellular antioxidant defense mechanisms and subsequently lead to a pro-oxidant condition.
Lee
M H
MH
Department of Biochemistry, College of Natural Sciences Kyungpook National University, Taegu, Korea.
Park
J W
JW
D002845:Chromatography / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004586:Electrophoresis / Q000379:methods; D004789:Enzyme Activation / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D005231:Fatty Acids, Unsaturated / Q000378:metabolism / Q000494:pharmacology*; D005609:Free Radicals; D008041:Linoleic Acids / Q000378:metabolism / Q000494:pharmacology; D015227:Lipid Peroxidation; D009829:Oleic Acids / Q000378:metabolism; D010084:Oxidation-Reduction; D010545:Peroxides / Q000494:pharmacology; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D013482:Superoxide Dismutase / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors; D020122:tert-Butylhydroperoxide
D005231:Fatty Acids, Unsaturated; D005609:Free Radicals; D008041:Linoleic Acids; D009829:Oleic Acids; D010545:Peroxides; D011506:Proteins; D017392:Thiobarbituric Acid Reactive Substances; C005575:methyl linoleate; C005576:methyl oleate; D020122:tert-Butylhydroperoxide; D013482:Superoxide Dismutase