YAC transgenics and the study of genetics and human disease.
Advances in yeast artificial chromosome (YAC) technologies over the past decade have enabled the precise identification and manipulation of large genomic regions (>100 kb) of DNA. Introduction of YACs into the mouse germline has now been accomplished through transfection of mouse embryonic stem cells as well as through pronuclear microinjection, allowing the efficient transfer defined genomic loci into mice. YAC transgenics will have a profound impact on the development of transgenic mice as bioreactors and as models of human disease, and on the functional analysis of higher order genomic structure.
Lamb
B T
BT
Department of Gynecology and Obsterics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287-2501, USA.
Gearhart
J D
JD
D000818:Animals; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics*; D004195:Disease Models, Animal; D015870:Gene Expression; D030342:Genetic Diseases, Inborn / Q000235:genetics*; D006801:Humans; D008322:Mammals / Q000235:genetics; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D008957:Models, Genetic; D013104:Spheroplasts / Q000235:genetics