The mouse prostaglandin E receptor EP2 subtype: cloning, expression, and northern blot analysis.
A functional cDNA clone for the mouse prostaglandin (PG) E receptor EP2 subtype was isolated from a mouse cDNA library. The mouse EP2 receptor consists of 362 amino acid residues with seven putative transmembrane domains. [3H]PGE2 bound specifically to the membrane of Chinese hamster ovary cells stably expressing the cloned receptor. This binding was displaced by unlabeled prostanoids in the order of PGE2 = PGE1 >> iloprost, a stable PGI2 agonist > PGF2 alpha > PGD2. Binding was also inhibited by butaprost (an EP2 agonist) and to a lesser extent by M&B 28767 (an EP3 agonist), but not by sulprostone (an EP1 and EP3 agonist) or SC-19220 (an EP1 antagonist). PGE2 and butaprost increased the cAMP level in the Chinese hamster ovary cells in a concentration-dependent manner. Northern blot analysis revealed that EP2 mRNA is expressed most abundantly in the uterus, followed by the spleen, lung, thymus, ileum, liver, and stomach.
Katsuyama
M
M
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
Nishigaki
N
N
Sugimoto
Y
Y
Morimoto
K
K
Negishi
M
M
Narumiya
S
S
Ichikawa
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D018014:Gene Transfer Techniques; D051379:Mice; D008969:Molecular Sequence Data; D011453:Prostaglandins / Q000378:metabolism; D011869:Radioligand Assay; D011982:Receptors, Prostaglandin / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D016415:Sequence Alignment
D011453:Prostaglandins; D011982:Receptors, Prostaglandin