Fibronectin attachment is permissive for IL-1 mediated gene regulation.
This study examines the effect of cell-matrix interaction on IL-1 induced gene regulation. In fibroblasts and smooth muscle cells attached to fibronectin, IL-1 caused a pronounced reduction in proteoglycan synthesis, while no reduction occurred in cells plated on bare plastic. Further, fibronectin attachment was permissive for IL-1 mediated suppression of both versican and collagen mRNA levels, initially noted after 4-6 h of IL-1 incubation. Attachment to vitronectin was less potent in influencing regulation, and collagen had no effect, suggesting specificity of the matrix modulation of the IL-1 induced response. Similar fibronectin induced dependence was demonstrated for IL-1 regulation of IL-6 gene expression, supporting the notion of a general effect of fibronectin receptor engagement on IL-1 induced signal transduction.
Ostberg
C O
CO
Department of Pathology, University of Washington, Seattle 98195, USA.
Zhu
P
P
Wight
T N
TN
Qwarnstrom
E E
EE
D002448:Cell Adhesion; D002478:Cells, Cultured; D005347:Fibroblasts / Q000502:physiology; D005353:Fibronectins / Q000378:metabolism*; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D009131:Muscle, Smooth, Vascular / Q000502:physiology; D011509:Proteoglycans / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D019096:Vitronectin
D005353:Fibronectins; D006023:Glycoproteins; D007375:Interleukin-1; D015850:Interleukin-6; D011509:Proteoglycans; D012333:RNA, Messenger; D019096:Vitronectin