Stimulatory effect of ursodeoxycholate on pancreatic exocrine secretion in an in vitro study.
Ursodeoxycholate (UDCA; 300 microM) significantly stimulates amylase secretion from isolated rat pancreatic acini but not Ca2+ mobilization. Removal of extracellular Ca2+ completely abolished the UDCA-stimulated secretory response. Ca ionophore (A23187) potentiated the response. However, staurosporine, a protein kinase C inhibitor, did not affect the UDCA-stimulated amylase secretion. These results indicated that UDCA directly acted on the pancreatic acini and stimulated amylase secretion by the mechanism of stimulation of influx of extracellular Ca2+, but not through inositol [1,4,5] triphosphate and diacylglycerol formation or Ca2+ mobilization.
Shinozaki
H
H
Social Insurance Inatsuki Hospital, Fukuoka, Japan.
Funakoshi
A
A
Miyasaka
K
K
Kitani
K
K
D000470:Alkaloids / Q000494:pharmacology; D000681:Amylases / Q000378:metabolism; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002766:Cholecystokinin / Q000494:pharmacology; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism; D017136:Ion Transport / Q000187:drug effects; D008297:Male; D010179:Pancreas / Q000187:drug effects* / Q000378:metabolism*; D010477:Perfusion; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar; D019311:Staurosporine; D014580:Ursodeoxycholic Acid / Q000494:pharmacology*
D000470:Alkaloids; D000001:Calcimycin; D014580:Ursodeoxycholic Acid; D002766:Cholecystokinin; D011493:Protein Kinase C; D000681:Amylases; D019311:Staurosporine; D002118:Calcium