title: In vitro effect of dipyrone on several peroxidase labelled immunoassays.
abstract: We studied the in vitro effect of dipyrone on the determination of free triiodothyronine (free T3), cortisol, progesterone, estradiol, carcinoembryonic antigen, human chorionic gonadotropin and alpha-fetoprotein measured with an immunoenzyme assay based on enhanced luminescence that uses peroxidase as label. We found significant interference from dipyrone (p < 0.01) in the determination of all the analytes mentioned: for progesterone and estradiol the interference was present at high doses of dipyrone; for free T3 and cortisol the minimum dipyrone concentration producing interference was 712 mumol/l and for carcinoembryonic antigen, human chorionic gonadotropin and alpha-fetoprotein 44 mumol/l. Dipyrone has an analytically and statistically significant interference effect on the determination of the mentioned analytes.
authors.affiliation: Servei de Bioquímica, Hospital de la Santa creu i Sant Pau, Barcelona, Spain.
authors.forename: N
authors.initials: N
authors.lastname: Gascón-Roche
authors.forename: J
authors.initials: J
authors.lastname: Mora-Brugués
authors.forename: J
authors.initials: J
authors.lastname: Rodriguez-Espinosa
authors.forename: M
authors.initials: M
authors.lastname: Cortés-Rius
authors.forename: F
authors.initials: F
authors.lastname: González-Sastre
mesh_terms: D016477:Artifacts; D002272:Carcinoembryonic Antigen / Q000097:blood; D006063:Chorionic Gonadotropin / Q000097:blood; D004177:Dipyrone / Q000494:pharmacology*; D004958:Estradiol / Q000097:blood; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007124:Immunoenzyme Techniques; D010544:Peroxidases / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000097:blood; D014284:Triiodothyronine / Q000097:blood; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D002272:Carcinoembryonic Antigen; D006063:Chorionic Gonadotropin; D000509:alpha-Fetoproteins; D014284:Triiodothyronine; D011374:Progesterone; D004958:Estradiol; D004177:Dipyrone; D010544:Peroxidases; D006854:Hydrocortisone