Binding of sequence-specific proteins to the 3'-untranslated region of vasoactive intestinal peptide mRNA.
Multimeric AUUUA elements in an AU-rich 3'-untranslated region (3'-UTR) have been shown to confer message instability to numerous ephemeral transcripts through the formation of RNA-protein complexes. We show here that the 3'-UTR of VIP mRNA, which contains 3 AUUUA motifs in an AU-rich context, forms specific complexes with cytoplasmic proteins in a concentration-dependent, tissue-specific manner. We also demonstrate that an AU-rich segment of c-fos mRNA can successfully compete with VIP mRNA for binding with cytoplasmic proteins. These studies provide the first evidence for a mechanism by which VIP is post-transcriptionally regulated through specific sequences in its 3'-UTR.
Wolford
J K
JK
Department of Pharmacology, Northeastern Ohio Universities College of Medicine, Rootstown 44272, USA.
Signs
S A
SA
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D003593:Cytoplasm / Q000737:chemistry; D005786:Gene Expression Regulation; D015698:Genomic Library; D008297:Male; D008969:Molecular Sequence Data; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011485:Protein Binding; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics; D012313:RNA / Q000378:metabolism; D012323:RNA Processing, Post-Transcriptional; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions; D014018:Tissue Distribution; D014660:Vasoactive Intestinal Peptide / Q000235:genetics*
D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; C039919:RNA, recombinant; D016601:RNA-Binding Proteins; D014660:Vasoactive Intestinal Peptide; D012313:RNA