title: Purine arabinonucleoside 5'-triphosphates with substituents at 2' position as substrates for DNA polymerases.
abstract: Analogues of araNTPs carrying an azido or aminogroup instead of the 2' hydroxyl exhibited substrate properties towards several mammalian and viral DNA polymerases. At the same time, introduction of a bulky hydrophobic DNP group into the 2' position inactivated the compounds as substrates. HSV-1 and CMV DNA polymerases were an interesting exception: they effectively incorporated the modified nucleotide residues with DNP group into the 3'-termini of the DNA chain. This is a reliable distinction of these enzymes from cellular DNA polymerases.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
authors.forename: Y A
authors.initials: YA
authors.lastname: Sharkin
authors.forename: L A
authors.initials: LA
authors.lastname: Alexandrova
authors.forename: L S
authors.initials: LS
authors.lastname: Victorova
authors.forename: E V
authors.initials: EV
authors.lastname: Kuznetsova
authors.forename: A A
authors.initials: AA
authors.lastname: Krayevsky
mesh_terms: D000588:Amines / Q000737:chemistry; D000818:Animals; D001088:Arabinonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001386:Azides / Q000737:chemistry; D001483:Base Sequence; D002417:Cattle; D003587:Cytomegalovirus / Q000201:enzymology; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004253:DNA Nucleotidylexotransferase / Q000737:chemistry / Q000378:metabolism; D004256:DNA Polymerase I / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004586:Electrophoresis; D018259:Herpesvirus 1, Human / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D010710:Phosphates / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D011685:Purine Nucleotides / Q000737:chemistry* / Q000378:metabolism*; D011687:Purines / Q000737:chemistry; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D000588:Amines; D001088:Arabinonucleotides; D001386:Azides; D017931:DNA Primers; D010710:Phosphates; D011685:Purine Nucleotides; D011687:Purines; D004247:DNA; D004253:DNA Nucleotidylexotransferase; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase