Molecular cloning of a cell-surface glycoprotein that can potentially discriminate mesothelium from epithelium: its identification as vascular cell adhesion molecule 1.
It has long been a practical problem for surgical pathologists to distinguish mesothelium from epithelium in order to make a positive diagnosis of mesothelioma. In this study, we developed a new monoclonal antibody, designated MS-2761 (IgG1, k), against cultured non-neoplastic mesothelial cells. Immunohistochemistry and slot-blot analysis revealed that this monoclonal antibody reacted with 100% (12/12) of benign and malignant mesothelioma tissues and a mesothelioma cell line, but not with 99% (77/78) of epithelial tumour tissues and 97% (33/34) of epithelial tumour cell lines. A gene encoding the cell-surface antigen defined by this monoclonal antibody was isolated from a mesothelial cell cDNA library constructed with a mammalian cell expression vector through transfection of Cos-7 cells and immunoselection by panning. DNA sequencing and a database search revealed that the gene was identical to vascular cell adhesion molecule 1 (VCAM1, also referred to as INCAM110). The prominent VCAM1 transcript in mesothelium was 3.2 kb in size with seven Ig-like domains, in addition to a minor transcripts with six Ig-like domains. This monoclonal antibody potentially discriminates mesothelium from epithelium and may become a tool for differential diagnosis of mesothelioma.
Yamada
T
T
Pathology Division, National Cancer Center Hospital, Tokyo, Japan.
Jiping
J
J
Endo
R
R
Gotoh
M
M
Shimosato
Y
Y
Hirohashi
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D001483:Base Sequence; D015152:Blotting, Northern; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004247:DNA / Q000032:analysis / Q000235:genetics; D004848:Epithelium / Q000737:chemistry / Q000276:immunology / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D008654:Mesothelioma / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D010997:Pleural Neoplasms / Q000737:chemistry / Q000473:pathology; D016133:Polymerase Chain Reaction; D011233:Precipitin Tests; D012194:RNA-Directed DNA Polymerase; D019010:Vascular Cell Adhesion Molecule-1
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D008562:Membrane Glycoproteins; D019010:Vascular Cell Adhesion Molecule-1; D004247:DNA; D012194:RNA-Directed DNA Polymerase