Synergistic effects of insulin-like growth factor II (IGF-II) with leukemia inhibiting factor (LIF) on establishment of rat pluripotential cell lines.
To examine effects of IGF-II on establishment of pluripotential diploid cells from rat embryos, we cultured blastocysts in medium containing mouse LIF with or without IGF-II. Combination of mouse LIF (5,000 units/ml) and rat IGF-II (100 ng/ml) promoted growth of inner cell mass (ICM) and was effective for establishment of pluripotential cell lines derived from the ICM. The cell lines indicated colony forms different from the rat ES cell lines. However, they showed morphological alteration to adult-like tissue cells, formed embryoid body in suspension culture, and thus, seemed to retain a pluripotent characteristics. The rat IGF-II is useful for establishing of pluripotential cells efficiently.
Takahashi
A
A
NT Science Co., Ltd., Tokyo, Japan.
Takahashi
Y
Y
Matsumoto
K
K
Miyata
K
K
D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D046508:Culture Techniques / Q000379:methods; D004357:Drug Synergism; D004622:Embryo, Mammalian; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000494:pharmacology*; D015850:Interleukin-6; D007700:Kinetics; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D051381:Rats; D011912:Rats, Inbred ACI; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D013045:Species Specificity; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects
D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D011994:Recombinant Proteins; D007335:Insulin-Like Growth Factor II