Calcium in pollen-pistil interaction in Petunia hybrida Hort. II. Localization of Ca2+ ions and Ca(2+)-ATPase in unpollinated pistil.
The localization of Ca2+ and Ca(2+)-ATPase activity in the unpollinated pistil of Petunia hybrida has been studied. Free or loosely sequestered Ca2+ ions have been found: (1) in the stigma exudate, (2) in the pistil transmitting tract, (3) on the surface of ovule, (4) in the cell wall of the embryo sac. The sites of Ca2+ sequestration in the transmitting cells were the plastids and vacuoles. In some cells, the Ca2+ ions were associated with the tonoplast and plasmalemma underlying the transverse walls which connect the transmitting cells. The sites of particular accumulation of Ca2+ were the surfaces of the placenta and of the ovule. In the ovule, numerous precipitates occurred in the extended walls connecting the embryo sac with the cells of the nucellus. Ca(2+)-ATPase was commonly localized on the plasmalemma (1) of the cells of the transmitting tract of the stigma and style, (2) of the placenta and (3) of the somatic cells of the ovule. The plasmalemma of the embryo sac cells was nearly completely devoid of the reaction product.
Bednarska
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Department of Plant Cytology and Embryology, N. Copernicus University, Toruń, Poland.
Butowt
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R
D000965:Antimony; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D004577:Electron Probe Microanalysis; D007202:Indicators and Reagents; D018521:Plant Physiological Phenomena; D010940:Plant Proteins / Q000378:metabolism*; D011058:Pollen
D007202:Indicators and Reagents; D010940:Plant Proteins; C009133:pyroantimonate; D000965:Antimony; D000252:Calcium-Transporting ATPases; D002118:Calcium