title: Rates of beta-oxidation of fatty acids of various chain lengths and degrees of unsaturation in highly purified peroxisomes isolated from rat liver.
abstract: Highly purified peroxisomes were obtained from the liver of untreated rats, and rates of peroxisomal beta-oxidation were measured using fatty acyl-CoAs differing in chain length and degree of unsaturation. A 20-24-fold purification of peroxisomes, indicated by the specific activities of the marker enzymes catalase and urate oxidase, respectively, was obtained from crude liver homogenate using differential centrifugation techniques followed by a 30% Nycodenz gradient separation. The use of a 30% Nycodenz gradient in the final step of purification was extremely effective (e.g. 5.5-fold reduction) in removing lysosomal contamination. The rate of peroxisomal beta-oxidation with lauroyl-CoA (C12:0) as substrate was the highest of all fatty acyl-CoAs tested. Butyryl-CoA (C4:0) was not oxidized by purified peroxisomes. In general, as chain length of the fatty acyl-CoAs increased above 12 carbons, the rates of beta-oxidation decreased.
authors.affiliation: Department of Food, Nutrition and Foodservice Management, University of North Carolina at Greensboro 27412, USA.
authors.forename: D S
authors.initials: DS
authors.lastname: Chance
authors.forename: M K
authors.initials: MK
authors.lastname: McIntosh
mesh_terms: D000818:Animals; D015415:Biomarkers; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000378:metabolism*; D008099:Liver / Q000737:chemistry* / Q000648:ultrastructure; D008297:Male; D008830:Microbodies / Q000737:chemistry*; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000737:chemistry
chemical_list: D015415:Biomarkers; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated