Genetic analysis of helper virus-specific selective amplification of cucumber mosaic virus satellite RNAs.
Satellite RNAs (sat-RNAs) are small molecular parasites associated with a number of plant RNA viruses. The cucumber mosaic virus (CMV) sat-RNAs are ca. 335 nucleotides and have evolved to produce a large number of closely related sat-RNAs. Different cucumoviruses can act as helper viruses in the amplification of CMV sat-RNAs. We have found that different helper viruses show a preference for a particular sat-RNA in a mixed infection. In this study the specificity of WL47 sat-RNA amplification by LS-CMV and of D4 sat-RNA amplification by tomato aspermy virus in mixed infections was examined. Recombinant cDNA clones of D4 sat-RNA and WL47 sat-RNA were used to map the sat-RNA sequences responsible for the helper virus selection of a specific sat-RNA for amplification.
Roossinck
M J
MJ
Plant Biology Division, S.R. Noble Foundation, Ardmore, OK 73402.
Palukaitis
P
P
D001483:Base Sequence; D017799:Cucumovirus / Q000235:genetics*; D018076:DNA, Complementary; D006378:Helper Viruses / Q000235:genetics*; D008969:Molecular Sequence Data; D012313:RNA / Q000235:genetics*; D019244:RNA, Satellite; D012641:Selection, Genetic; D016415:Sequence Alignment
D018076:DNA, Complementary; D019244:RNA, Satellite; D012313:RNA