title: Retardation of a surface protein chimera at the cis Golgi.
abstract: Dipeptidyl peptidase IV (D4) and the alpha subunit of human chorionic gonadotrophin (alpha hcg) are plasma membrane and secretory proteins, respectively. In the course of studies to understand mechanisms involved in transport along the exocytotic pathway, the ectoplasmic domain of D4 was replaced by the mature polypeptide of alpha hcg, resulting in the membrane anchored chimera, D4 alpha hcg. Surprisingly, when transfected into Chinese hamster ovary (CHO) and Madin-Darby canine kidney (MDCK) cells, strong perinuclear Golgi staining was predominant, in addition to the expected surface staining. By following the biogenesis and transport of the molecule, it was established that newly synthesized D4 alpha hdg is eventually transported to the cell surface but only after a significant retardation in the Golgi apparatus. The compartment of retardation was identified as the early or cis Golgi, before the medial Golgi, where resistance to endoglycosidase (endo) H is conferred. As a result of the transport retardation of the chimera, we were able to document the appearance of an endo D sensitive intermediate, which is usually too transient to be apparent in normal cells. The retardation of this chimera in the cis Golgi complements our previous report in which the D4 molecule with its transmembrane domain replaced by that of aminopeptidase N resulted in retardation in the trans Golgi/trans Golgi network in MDCK and CHO cells [Low, S. H., Tang, B. L., Wong, S. H., & Hong, W. (1994) J. Biol. Chem. 269, 1985-1994).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Membrane Biology Laboratory, National University of Singapore.
authors.forename: S H
authors.initials: SH
authors.lastname: Low
authors.forename: B L
authors.initials: BL
authors.lastname: Tang
authors.forename: S H
authors.initials: SH
authors.lastname: Wong
authors.forename: W
authors.initials: W
authors.lastname: Hong
mesh_terms: D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006224:Cricetinae; D017931:DNA Primers; D018819:Dipeptidyl Peptidase 4 / Q000096:biosynthesis / Q000378:metabolism*; D004285:Dogs; D005455:Fluorescent Antibody Technique; D015292:Glycoprotein Hormones, alpha Subunit / Q000096:biosynthesis / Q000378:metabolism*; D006056:Golgi Apparatus / Q000378:metabolism*; D006801:Humans; D007668:Kidney; D007700:Kinetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism*; D017693:Sodium Bicarbonate / Q000494:pharmacology; D014162:Transfection
chemical_list: D017931:DNA Primers; D015292:Glycoprotein Hormones, alpha Subunit; D011993:Recombinant Fusion Proteins; D017693:Sodium Bicarbonate; D018819:Dipeptidyl Peptidase 4