Reduction of exogenous ferric iron by a surface-associated ferric reductase of Listeria spp.
The reduction of exogenous ferric iron by Listeria monocytogenes, a Gram-positive food-borne pathogen, was investigated. Using an assay incorporating the ferrous iron chelator ferrozine, we showed that intact cells of L. monocytogenes, when exposed to ferric iron, were able to rapidly reduce and solubilize the iron to the ferrous form. Reduction occurred only after direct contact between the bacteria and the iron source. A number of different ferric iron chelates, including transferrin and lactoferrin-bound iron, haemoglobin, ferritin, and iron complexed to siderophores, could be reduced. The ferric reductase activity was expressed by both reference strains and clinical isolates of L. monocytogenes and by all other species of Listeria, although significant quantitative differences were observed. In L. monocytogenes, the expression of ferric reductase was not affected by the growth phase of the bacteria nor by the presence or absence of iron in the growth medium. However, expression was greatly reduced in bacteria grown anaerobically and when cultured in media of reduced pH. In addition, bacteria grown at a cold temperature displayed greater ferric reductase activity than cells grown at higher temperatures. A surface-associated ferric reductase system may be one component of a general iron scavenging mechanism which can be used by Listeria growing in a variety of environments.
Deneer
Harry G
HG
Department of MicrobiologyUniversity of SaskatchewanSaskatoon, Saskatchewan, Canada, S7N 5E5.
Healey
Vanessa
V
Division of Clinical MicrobiologyRoyal University Hospital Saskatoon, Saskatchewan, Canada, S7N 5E5.
Boychuk
Irene
I
Department of MicrobiologyUniversity of SaskatchewanSaskatoon, Saskatchewan, Canada, S7N 5E5.
D000332:Aerobiosis; D001426:Bacterial Proteins / Q000378:metabolism*; D003067:Coenzymes / Q000737:chemistry; D038181:FMN Reductase; D005290:Ferric Compounds / Q000378:metabolism*; D005297:Ferrozine / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007502:Iron Chelating Agents / Q000378:metabolism; D008087:Listeria / Q000235:genetics / Q000378:metabolism; D008089:Listeria monocytogenes / Q000254:growth & development / Q000378:metabolism*; D008274:Magnesium / Q000737:chemistry; D008565:Membrane Proteins / Q000378:metabolism*; D008667:Metalloproteins / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000096:biosynthesis / Q000378:metabolism*; D010084:Oxidation-Reduction; D013696:Temperature; D013997:Time Factors
D001426:Bacterial Proteins; D003067:Coenzymes; D005290:Ferric Compounds; D007502:Iron Chelating Agents; D008565:Membrane Proteins; D008667:Metalloproteins; D005297:Ferrozine; D038181:FMN Reductase; D009247:NADH, NADPH Oxidoreductases; C023903:ferric citrate iron reductase; D008274:Magnesium