[Immunochemical determination of saccharin].
Saccharin is coupled to bovine serum albumin by spacers via the five membered as well as the six membered ring. Rabbits treated with these immunogenic conjugates give precipitating antibodies which are used for coating polystyrol tubes and microtiter plates. Saccharin is determined down to 0.1 mg/l, the detector reaction being performed by a saccharin horseradish peroxidase conjugate and hydrogen peroxide/1,2-diaminobenzen as substratum.
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Institut für Pharmazeutische Chemie, Westfälischen Wilhelms-Universität Münster.
Baudner
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Gerke
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Kütter
M
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Langfermann
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C
D000818:Animals; D002417:Cattle; D006735:Horseradish Peroxidase / Q000737:chemistry; D018796:Immunoconjugates / Q000737:chemistry; D007124:Immunoenzyme Techniques; D011817:Rabbits / Q000276:immunology; D012439:Saccharin / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology / Q000378:metabolism
D018796:Immunoconjugates; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D006735:Horseradish Peroxidase; D012439:Saccharin