A serotype-specific epitope of dengue virus 1 identified by phage displayed random peptide library.
From a panel of monoclonal antibodies of dengue viruses, a serotype-specific epitope of dengue virus 1 was screened from a random peptide library displayed on phage. The epitope was the determinant reactive with monoclonal antibody 15F3-1 that was specific to dengue 1. The screening was monitored by a dot blotting procedure, and after three rounds of screening a consensus motif, HRYSWK, was found. This sequence matches the sequence HKYSWK, corresponding to the amino acid residues 885-890 of polyprotein or residues 111-116 of the non-structural protein 1 of dengue virus serotype 1. The linear epitope was confirmed by testing the antigenicity of chemically synthesized 8-branched peptide.
Yao
Z J
ZJ
Bioprocessing Technology Centre, National University of Singapore.
Kao
M C
MC
Loh
K C
KC
Chung
M C
MC
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D001667:Binding, Competitive; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D003716:Dengue Virus / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D000939:Epitopes / Q000235:genetics; D015723:Gene Library; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012703:Serotyping; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D017931:DNA Primers; D004279:DNA, Viral; D000939:Epitopes; D009842:Oligopeptides; D014764:Viral Proteins