Cloning and characterization of pentylenetetrazol-related cDNA, PTZ-17.
cDNAs related to pentylenetetrazol-induced bursting activity in neurons were screened by a differential hybridization method using normal and pentylenetetrazol-treated primary cultured neurons from the cerebral cortex of mice. Twenty clones of candidate cDNA with expression increased or decreased by treatment with pentylenetetrazol were obtained. One of them, PTZ-17, was sequenced. Injection of PTZ-17 derived RNA into Xenopus oocytes showed a large calcium inward current with extracellular application of pentylenetetrazol.
Kajiwara
K
K
Department of Physiology, Kanagawa Dental College, Yokosuka, Japan.
Sugaya
E
E
Kimura
M
M
Katsuki
M
M
Nagasawa
H
H
Yuyama
N
N
Tsuda
T
T
Sugaya
A
A
Motoki
M
M
Ookura
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects*; D010433:Pentylenetetrazole / Q000378:metabolism*; D012313:RNA / Q000008:administration & dosage / Q000187:drug effects; D014981:Xenopus
D018076:DNA, Complementary; D012313:RNA; D002118:Calcium; D010433:Pentylenetetrazole