Recombinant human granulocyte colony-stimulating factor does not influence distribution or sister chromatid exchange frequency of proliferating mononuclear cells of the peripheral blood.
Phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMC) were treated with increasing concentrations of filgrastim, the unglycosylated methionine granulocyte colony-stimulating factor of man (rhG-CSF), and cultured for 72 h. There were no impaired proliferation or differentiation of proliferating PBMC, no impaired expression of activation markers such as the low-affinity interleukin 2 receptor and transferrin receptor, and no induction of sister chromatid exchanges. Under these conditions, no effects of a general DNA destabilization of peripheral blood leukocytes was observed. Thus, longterm administration of therapeutical concentrations of rhG-CSF should not produce severe mutagenic effects.
Büssing
A
A
Institute of Medical Immunology, Faculty of Medicine, Technical University Aachen, Germany.
Azhari
T
T
Mertens
R
R
Schweizer
K
K
D000328:Adult; D002455:Cell Division / Q000187:drug effects; D005260:Female; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000502:physiology; D008297:Male; D011994:Recombinant Proteins / Q000494:pharmacology; D012016:Reference Values; D012854:Sister Chromatid Exchange / Q000187:drug effects* / Q000502:physiology
D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor