title: Comparative biochemical study of coated vesicles purified from cock brains.
abstract: Cock brain coated vesicles (CBCVs) were purified and compared with porcine brain coated vesicles (PBCVs) from several biochemical aspects. Clathrin heavy and light chains of CBCVs were immunologically similar to those of PBCVs. Coat proteins (CPs) of CBCVs behaved in almost the same manner as those of PBCVs for limited proteolysis. Dissociation of CPs from CBCVs by treatment with 10 mM Tris-Cl, pH 8.5, or 2M urea resembled that of CPs from PBCVs. pH dependency of dissociation of CPs from CBCVs was slightly different from those of PBCVs.
authors.affiliation: Department of Medical Technology, Saitama Medical School Junior College, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yora
authors.forename: Y
authors.initials: Y
authors.lastname: Sakagishi
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D002417:Cattle / Q000276:immunology / Q000378:metabolism; D002645:Chickens / Q000276:immunology / Q000378:metabolism*; D002966:Clathrin / Q000032:analysis / Q000276:immunology; D003034:Coated Pits, Cell-Membrane / Q000737:chemistry*; D004705:Endocytosis; D000939:Epitopes / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000276:immunology; D010196:Pancreatic Elastase / Q000378:metabolism; D010449:Peptide Mapping; D011817:Rabbits; D013045:Species Specificity; D013552:Swine / Q000276:immunology / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: D002966:Clathrin; D000939:Epitopes; D007106:Immune Sera; D008565:Membrane Proteins; D010196:Pancreatic Elastase; D014357:Trypsin