The first observation of O2- generation at real time in vivo from non-Kupffer sinusoidal cells in perfused rat liver during acute ethanol intoxication.
Infusion of ethanol or phorbol myristate acetate (PMA) into the perfused rat liver immediately produces O2- which was detected directly by infusion of a Cypridina luciferin analogue, MCLA as a chemiluminescence reagent. The MCLA photon emission was inhibitable by SOD. Generation of O2- in the liver was further verified by nitroblue tetrazolium, formazan precipitate formation. Ethanol-induced O2- generation was unaffected by gadolinium chloride (GdCl3), an inhibitor of kupffer cells, while PMA induced O2- generation was completely abolished by GdCl3. Since PMA is a known stimulator of phagocytic cells including Kupffer cells, the results indicate, for the first time that ethanol stimulates a non-Kupffer cell population, probably liver sinusoid endothelial cell to produce O2-.
Nakano
M
M
Department of Photon and Free Radical Research, Japan Immunoresearch Laboratories Co. Ltd., Gunma.
Kikuyama
M
M
Hasegawa
T
T
Ito
T
T
Sakurai
K
K
Hiraishi
K
K
Hashimura
E
E
Adachi
M
M
D000818:Animals; D004727:Endothelium / Q000378:metabolism / Q000473:pathology; D000431:Ethanol / Q000633:toxicity*; D007093:Imidazoles; D007728:Kupffer Cells / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010477:Perfusion; D011719:Pyrazines; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism*
D007093:Imidazoles; D011719:Pyrazines; D013481:Superoxides; C063412:2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo(1,2-alpha)pyrazin-3-one; D000431:Ethanol