title: Quantitative immunohistochemical determination of cathepsin D levels in prostatic carcinoma biopsies. Correlation with tumor grade, stage, PSA level, and DNA ploidy status.
abstract: National screening programs resulting in an increased detection rate of prostatic adenocarcinoma have prompted the search for new methods of predicting disease outcome that can be applied to the initial narrow bore needle biopsy specimens. Cathepsin D, a lysosomal aspartyl protease and autocrine mitogen, has been studied in a wide variety of human neoplasms as an invasion and metastasis marker. Prostatic carcinoma needle biopsy tumor cell cathepsin D content was measured in 61 men using a semiquantitative image analysis assisted immunohistochemical procedure. Results were compared with preoperative serum prostatic specific antigen levels, tumor grade, DNA ploidy status, pathologic stage after radical prostatectomy and disease recurrence during a median 2.6 year follow-up. Biopsy cathepsin D levels significantly correlated with tumor grade (P = .022) and DNA ploidy status (P = .028) by logistic regression analysis. Post-prostatectomy pathologic stage and disease recurrence did not correlate with tumor cathepsin D levels. Final prostatectomy grade and DNA ploidy status independently predicted metastasis and post-operative disease recurrence (P < .001). Although this study did not find independent prognostic status for cathepsin D in prostate cancer, the correlation with tumor grade and DNA ploidy status is noteworthy and the inter-relationship of outcome variables may prove of interest and warrant further evaluation of this potential predictor or CO-predictor of disease outcome.
authors.affiliation: Department of Pathology, Albany Medical College, NY 12208, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Ross
authors.forename: T
authors.initials: T
authors.lastname: Nazeer
authors.forename: H L
authors.initials: HL
authors.lastname: Figge
authors.forename: H A
authors.initials: HA
authors.lastname: Fisher
authors.forename: M D
authors.initials: MD
authors.lastname: Rifkin
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000737:chemistry / Q000473:pathology*; D000328:Adult; D000368:Aged; D001707:Biopsy, Needle; D002402:Cathepsin D / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D011003:Ploidies; D011379:Prognosis; D011467:Prostate / Q000473:pathology / Q000601:surgery; D017430:Prostate-Specific Antigen / Q000097:blood*; D011468:Prostatectomy; D011471:Prostatic Neoplasms / Q000097:blood / Q000737:chemistry / Q000473:pathology*
chemical_list: D004273:DNA, Neoplasm; D017430:Prostate-Specific Antigen; D002402:Cathepsin D