Immunocytochemical localization of 155 kDa myosin light chain kinase and myosin heavy chain in bovine brain.
We immunocytochemically stained the bovine brain with monoclonal antibodies against 155 kDa MLCK and myosin heavy chain (MHC) and a polyclonal antibody against calmodulin. In the bovine brain, a strong immunoreactivity with the anti-MLCK antibody was observed in neurons in all layers of the cerebral cortex and basal ganglia, and astrocytes in the white matter. The antibody also stained Purkinje, granular and molecular cells in the cerebellum. An anti MHC antibody stained neurons and astrocytes in the cerebral cortex and Purkinje cells similarly to the anti-MLCK antibody, whereas the MHC immunoreactivity was detected in the cerebellar glomerulus and there was no immunostaining of MHC in the granular and outer stellate cells. These results suggest that the 155 kDa MLCK might be related not only to the Ca-calmodulin-myosin system but also other intracellular system.
Shimada
H
H
Second Department of Internal Medicine, Osaka City University, Japan.
Shimizu
T
T
Kuwayama
H
H
Suzuki
M
M
Nagai
R
R
Morii
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D001923:Brain Chemistry / Q000502:physiology; D002417:Cattle; D007150:Immunohistochemistry; D008970:Molecular Weight; D018995:Myosin Heavy Chains / Q000378:metabolism*; D009219:Myosin-Light-Chain Kinase / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology
D000911:Antibodies, Monoclonal; D009219:Myosin-Light-Chain Kinase; D018995:Myosin Heavy Chains