title: Expression of CD44 variant exons 8-10 in colorectal cancer and its relationship to metastasis.
abstract: Splice variants of CD44 are overexpressed in human lung, breast, and colon carcinoma cell lines. This study was conducted to clarify the association between the expression of CD44 variant exons 8-10 and metastatic potential in human colorectal cancer. We found that the expression of a CD44 splice variant containing exons v8-10 was increased in all of 60 colorectal cancer specimens examined compared with matched normal colerectal mucosa, as determined by Northern blotting. Expression of CD44 variant exons 8-10 did not significantly correlate with histological type, depth of tumor invasion, lymphatic invasion, venous invasion, or lymph node metastasis. However, the level of CD44 variant exon 8-10 expression was significantly higher in carcinomas associated with liver metastasis than in those without liver metastasis. In addition, expression of CD44 variant exons 8-10 in the liver metastases was more intense than that in the primary colorectal cancers. These findings indicated that this domain of the CD44 glycoprotein encoded by exons v8-10 may play an important role in tumor hematogenous metastasis of human colorectal cancer.
authors.affiliation: First Department of Surgery, Fukui Medical School.
authors.forename: K
authors.initials: K
authors.lastname: Takeuchi
authors.forename: A
authors.initials: A
authors.lastname: Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Urano
authors.forename: T
authors.initials: T
authors.lastname: Goi
authors.forename: G
authors.initials: G
authors.lastname: Nakagawara
authors.forename: H
authors.initials: H
authors.lastname: Shiku
mesh_terms: D015152:Blotting, Northern; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D003110:Colonic Neoplasms / Q000473:pathology*; D005091:Exons; D015870:Gene Expression; D006801:Humans; D018960:Hyaluronan Receptors; D008113:Liver Neoplasms / Q000556:secondary; D008207:Lymphatic Metastasis; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D016201:Receptors, Lymphocyte Homing / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D012004:Rectal Neoplasms / Q000473:pathology*; D013329:Structure-Activity Relationship
chemical_list: D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing