title: [Detection of mixed lymphoid chimerism after allogeneic bone marrow transplantation: demonstration by interphase cytogenetics in paraffin-embedded tissue].
abstract: In bone marrow transplantation (BMT) the detection of residual host lymphoid or haematopoietic cells surviving conditioning therapy is because of its association to graft-versus-host disease, graft-versus-leukemia reaction, and relapse of leukemia a matter of great interest. We studied the occurrence of this mixed lymphoid chimerism (MC) in the formol-fixed lymphatic tissue of lymph nodes and spleen from 21 autopsies after allogeneic sex-mismatched BMT (5 females, 16 males, survival 5 to 1140 days after BMT). In situ hybridisation with biotinylated centromer-specific anti-X- and anti-Y-chromosome probes was performed on pepsin-digested paraffin sections. The number of double X-, single X-, and Y-chromosome bearing cells was analysed microscopically. Because of artefacts only 14 cases remained for valid investigation. MC was detected in 6 cases (5 out of 11 males 5 days to 840 days and 1 out of 3 females 76 days after BMT). MC occurred after whole body irradiation with 10 Gy (n = 5) and 7 Gy (n = 1). In 1 autopsy relapse of leukemia caused host cell infiltration. Cases with MC did not express histological signs of acute or chronic graft-versus-host disease, but 5 out of 8 with complete lymphoid chimerism did. The sensitivity of interphase cytogenetics on paraffin embedded tissue is low.
authors.affiliation: Institut für Pathologie, Universität Leipzig.
authors.forename: T
authors.initials: T
authors.lastname: Friedrich
authors.forename: G
authors.initials: G
authors.lastname: Ott
authors.forename: J
authors.initials: J
authors.lastname: Kalla
authors.forename: W
authors.initials: W
authors.lastname: Helbig
authors.forename: H
authors.initials: H
authors.lastname: Schwenke
authors.forename: M
authors.initials: M
authors.lastname: Kubel
authors.forename: W
authors.initials: W
authors.lastname: Pönisch
authors.forename: P
authors.initials: P
authors.lastname: Feyer
authors.forename: A
authors.initials: A
authors.lastname: Friedrich
mesh_terms: D001344:Autopsy; D016026:Bone Marrow Transplantation / Q000276:immunology* / Q000401:mortality / Q000473:pathology*; D002678:Chimera; D003582:Cytogenetics; D005260:Female; D006650:Histocompatibility Testing; D006652:Histological Techniques; D006801:Humans; D017403:In Situ Hybridization; D008214:Lymphocytes / Q000276:immunology* / Q000473:pathology; D008297:Male; D010232:Paraffin; D012727:Sex Characteristics; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D010232:Paraffin