Adrenocorticotropin activates glycerol-3-phosphate acyltransferase in bovine adrenal glomerulosa cells.
1. The mechanism whereby ACTH activates the synthesis of diacylglycerol (DAG) in isolated adrenal glomerulosa cells was investigated. 2. ACTH activates glycerol-3-phosphate acyltransferase (G3PAT) in intact and cell-free preparations of adrenal glomerulosa cells. Whereas activation of G3PAT by ACTH was observed in homogenates and membrane fractions, no activation was observed by angiotensin II (AII) at the same concentration. 3. ACTH effects were mimicked by nonspecific phospholipase C (PLC). 4. Our preliminary results suggest that ACTH activation of G3PAT may account for ACTH-induced increases in DAG via de novo synthesis of phosphatidic acid (PA).
Foster
R H
RH
Department of Physiology and Biophysics, Faculty of Medicine, University of Chile, Santiago.
Veitl
S
S
D000214:Acyl Coenzyme A / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D004075:Diglycerides / Q000096:biosynthesis; D004789:Enzyme Activation / Q000187:drug effects; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism; D010738:Type C Phospholipases / Q000494:pharmacology; D015384:Zona Glomerulosa / Q000187:drug effects / Q000201:enzymology*
D000214:Acyl Coenzyme A; D004075:Diglycerides; D010712:Phosphatidic Acids; D000804:Angiotensin II; D000324:Adrenocorticotropic Hormone; D005992:Glycerol-3-Phosphate O-Acyltransferase; D010738:Type C Phospholipases