Is nephrocalcin related to the urinary derivative (bikunin) of inter-alpha-trypsin inhibitor?
OBJECTIVE
To isolate, purify, sequence and characterize nephrocalcin (NC), a urinary protein that may be an important determinant of calcium oxalate (CaOx) kidney-stone disease.


METHODS
Proteins were isolated from human urine using cellulose and resin columns and were sequenced using Edman degradation and SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Inhibition of CaOx crystal growth by the isolated proteins was assessed by measuring the deposition of 14C-labelled CaOx.


RESULTS
A protein assumed to be NC on the basis of SDS-PAGE, inhibitory and gel filtration properties was isolated from healthy human urine. Its molecular weight and the amino acid sequences of two of its peptides suggested it was identical to fragment HI-14 of the light chain (bikunin) of inter-alpha-trypsin inhibitor (ITI).


CONCLUSIONS
NC represents a portion of the light chain of ITI, although this conclusion must remain tentative until confirmed using authentic NC.
Tang
Y
Y
Department of Surgery, Flinders Medical Centre, Bedford Park, Australia.
Grover
P K
PK
Moritz
R L
RL
Simpson
R J
RJ
Ryall
R L
RL
D000510:Alpha-Globulins / Q000737:chemistry; D000595:Amino Acid Sequence; D002129:Calcium Oxalate / Q000037:antagonists & inhibitors*; D002852:Chromatography, Ion Exchange; D006023:Glycoproteins / Q000652:urine*; D006801:Humans; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D011480:Protease Inhibitors / Q000652:urine*; D014360:Trypsin Inhibitor, Kunitz Soybean; D014361:Trypsin Inhibitors / Q000737:chemistry; D000515:alpha 1-Antitrypsin
D000510:Alpha-Globulins; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D011480:Protease Inhibitors; C109148:SPINT2 protein, human; D014361:Trypsin Inhibitors; D000515:alpha 1-Antitrypsin; C029490:nephrocalcin; D002129:Calcium Oxalate; C011877:inter-alpha-inhibitor; D014360:Trypsin Inhibitor, Kunitz Soybean