[Reconfirmation of golgiphobic dendrites of the motoneurons in rat spinal cord].
With CB-HRP method (injections into 5 muscles of anterior and posterior extremities) and Golgi technique, the corresponding sections of spinal cords were observed on same aged rats of the identical parent rats. Comparing the cell numbers of the lateral groups of anterior horns, the former (CB-HRP) revealed twice as many as the cell numbers of the latter (Golgi). As to the surface densities of the white matter dendrites (WMD) in the lateral funiculi from the neurons of the lateral groups of the anterior horns, the density revealed by injection of CB-HRP to the tibialis anterior is 2-9 times more than that in Golgi sections. All of WMD revealed in CB-HRP sections could extend into the peripheral portions of the lateral funiculi, and quite a few of them even form a subpial marginal plexus, that is one example of Golgiphobic dendrites (GBD). For labeled medial cell groups of the anterior horn with CB-HRP, their dendrites could reach to the ependymal layer of the central canal (another GBD). These two types of GBD were not present on the Golgi material. The significance of GBD was also discussed.
Yang
T
T
Institute of Basic Medical Sciences, CAMS, Beijing.
Shi
G
G
Wan
X
X
D000818:Animals; D002772:Cholera Toxin; D003712:Dendrites; D005260:Female; D006735:Horseradish Peroxidase; D008297:Male; D009046:Motor Neurons / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000033:anatomy & histology*; D013194:Staining and Labeling
C052635:cholera toxin, B subunit-horseradish peroxidase; D002772:Cholera Toxin; D006735:Horseradish Peroxidase