Low immunogenicity of therapeutic rat CD45 antibodies when used for pre-treatment of donor organs for transplantation.
Immunoassays for the measurement of human anti-immunoglobulin responses against the CD45 specific rat monoclonals, YTH 24.5 and YTH 54.12, have been developed. The assays are based on a 'double antigen' ELISA system and are reproducible with coefficients of variation of less than 15%. The assays were used to measure anti-immunoglobulin responses in sera from 40 patients who had received kidneys pre-treated with the pair of anti-CD45 monoclonals YTH 24.5 and YTH 54.12. Only two patients elicited a weak human antimurine antibody (HAMA) response.
Watts
M J
MJ
Cantab Pharmaceuticals Research Limited, Cambridge, UK.
Wisdom
N M
NM
Tyrrell
D A
DA
Hale
G
G
Connor
S E
SE
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000097:blood*; D000911:Antibodies, Monoclonal / Q000009:adverse effects* / Q000627:therapeutic use; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000706:statistics & numerical data; D006084:Graft Rejection / Q000276:immunology; D006801:Humans; D017493:Leukocyte Common Antigens / Q000276:immunology*; D051379:Mice; D051381:Rats; D015203:Reproducibility of Results; D012217:Rheumatoid Factor / Q000097:blood; D014181:Transplantation Immunology
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D012217:Rheumatoid Factor; D017493:Leukocyte Common Antigens