Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme.
The interleukin-1 beta (IL-1 beta) converting enzyme (ICE) processes the inactive IL-1 beta precursor to the proinflammatory cytokine. Adherent monocytes from mice harboring a disrupted ICE gene (ICE-/-) did not export IL-1 beta or interleukin-1 alpha (IL-1 alpha) after stimulation with lipopolysaccharide. Export of tumor necrosis factor-alpha and interleukin-6 (IL-6) from these cells was also diminished. Thymocytes from ICE-/- mice were sensitive to apoptosis induced by dexamethasone or ionizing radiation, but were resistant to apoptosis induced by Fas antibody. Despite this defect in apoptosis, ICE-/- mice proceed normally through development.
Kuida
K
K
Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510, USA.
Lippke
J A
JA
Ku
G
G
Harding
M W
MW
Livingston
D J
DJ
Su
M S
MS
Flavell
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D001483:Base Sequence; D020170:Caspase 1; D002478:Cells, Cultured; D002678:Chimera; D003546:Cysteine Endopeptidases / Q000172:deficiency / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D007375:Interleukin-1 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009000:Monocytes / Q000276:immunology*; D009550:Nigericin / Q000494:pharmacology; D013601:T-Lymphocytes / Q000166:cytology*; D019014:fas Receptor
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D016207:Cytokines; D007375:Interleukin-1; D008070:Lipopolysaccharides; D019014:fas Receptor; D003907:Dexamethasone; D003546:Cysteine Endopeptidases; D020170:Caspase 1; D009550:Nigericin