Crystallization and preliminary X-ray investigation of lipoxygenase-3 from soybeans.
Soybean lipoxygenase-3 has been crystallized by the vapor diffusion method in 16-20% polyethylene glycol (average M(r), 3,400), 0.2 M sodium acetate buffer, pH 5.7, at 21 degrees C, at a protein concentration of 8-15 mg/mL. The crystals, which diffract to 3-A spacings, belong to the monoclinic space group C2. Cell constants are a = 111.9, b = 136.4, and c = 61.6 A and beta = 95.7 degrees. The calculated value of Matthews's constant, Vm = 2.48 A3/kDa, is consistent with the presence of one molecule of lipoxygenase per crystallographic asymmetric unit (Z = 4).
Steczko
J
J
Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Minor
W
W
Stojanoff
V
V
Axelrod
B
B
D000595:Amino Acid Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D008084:Lipoxygenase / Q000737:chemistry*; D008969:Molecular Sequence Data; D013025:Glycine max / Q000201:enzymology*
C095864:lipoxygenase 3; D008084:Lipoxygenase