title: Detection of Bacteroides fragilis in clinical specimens by polymerase chain reaction amplification of the neuraminidase gene.
abstract: The polymerase chain reaction (PCR) was used in an attempt to detect Bacteroides fragilis by amplifying a segment of the gene encoding B. fragilis neuraminidase. Forty-five reference strains representing 45 species and 113 clinical isolates were tested. Only B. fragilis was PCR positive, except for Bacteroides merdae ATCC 43184, which gave a band by ethidium bromide staining that showed no signal by Southern hybridization. Using a protocol that employed DNA extraction by Sepa Gene kit and a highly sensitive digoxigenin-chemiluminescence detection system, detection of B. fragilis by PCR was in complete agreement with culture results for 44 clinical specimens from which a wide range of aerobic and anaerobic organisms and fungi were recovered.
authors.affiliation: Institute of Anaerobic Bacteriology, Gifu University School of Medicine, Japan.
authors.forename: R
authors.initials: R
authors.lastname: Jotwani
authors.forename: N
authors.initials: N
authors.lastname: Kato
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Ueno
mesh_terms: D001424:Bacterial Infections / Q000382:microbiology*; D001426:Bacterial Proteins / Q000032:analysis* / Q000235:genetics; D001441:Bacteroides fragilis / Q000201:enzymology / Q000302:isolation & purification*; D001483:Base Sequence; D006801:Humans; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000032:analysis* / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: D001426:Bacterial Proteins; D009439:Neuraminidase