title: Structures of the O-specific polysaccharide chains of Pectinatus cerevisiiphilus and Pectinatus frisingensis lipopolysaccharides.
abstract: Mild acid hydrolysis of the smooth-type lipopolysaccharide (LPS) of Pectinatus frisingensis afforded no polysaccharide but monomeric 6-deoxy-L-altrose (L-6dAlt) which was identified by anion-exchange chromatography in borate buffer, GLC/MS, 1H-NMR spectroscopy, and optical rotation. LPS was degraded with alkali under reductive conditions to give a completely O-deacylated polysaccharide, which was studied by methylation analysis, 1H-NMR and 13C-NMR spectroscopy, including sequential, selective spin-decoupling, two-dimensional correlation spectroscopy (COSY), COSY with relayed coherence transfer, two-dimensional heteronuclear 13C, 1H-COSY, one-dimensional NOE and two-dimensional rotating-frame NOE spectroscopy. It was found that the O-specific polysaccharide chain of P. frisingensis LPS is a homopolymer of 6-deoxy-L-altrofuranose built up of tetrasaccharide-repeating units having the following structure: [sequence: see text] Similarly, mild acid degradation of smooth-type LPS of Pectinatus cerevisiiphilus resulted in depolymerisation of the polysaccharide chain to give a disaccharide consisting of D-glucose and D-fucose. Study of the disaccharide by methylation analysis and alkali-degraded LPS by one-dimensional and two-dimensional 1H-NMR and 13C-NMR spectroscopy showed that the O-specific polysaccharide of P. cerevisiiphilus has the following structure: -->2)-beta-D-Fucf-(1-->2)-alpha-D-Glcp-(1-->.
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow.
authors.forename: S N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: A P
authors.initials: AP
authors.lastname: Moran
authors.forename: I M
authors.initials: IM
authors.lastname: Helander
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D001421:Bacteria, Anaerobic / Q000737:chemistry* / Q000276:immunology; D002240:Carbohydrate Sequence; D003837:Deoxy Sugars / Q000737:chemistry; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification; D008401:Gas Chromatography-Mass Spectrometry; D006601:Hexoses / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D003837:Deoxy Sugars; D004187:Disaccharides; D006601:Hexoses; D008070:Lipopolysaccharides; D019081:O Antigens; C053979:6-deoxyaltrose