An immunofluorescence assay in microwell plate (IFA) for detecting serum antibodies to Chlamydia pneumoniae.
An indirect immunofluorescence assay using a 96 microwell cell culture plate (IFA) was developed for the detection of antibodies to C. pneumoniae. The results obtained by IFA on 230 sera of healthy subjects were compared with those obtained with the microimmunofluorescence test. The correlation coefficient for IgG detection with the two methods was 0.96 showing good agreement.
Rumpianesi
F
F
Institute of Microbiology, University of Bologna, St. Orsola Hospital, Italy.
Donati
M
M
Pavan
G
G
Graciotti
C
C
Cevenini
R
R
D000328:Adult; D000368:Aged; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000276:immunology; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008297:Male; D008860:Microscopy, Ultraviolet; D008875:Middle Aged
D000888:Antibodies, Anti-Idiotypic; D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M