Epitope-tag vectors for eukaryotic protein production.
We report on the construction and use of two eukaryotic expression vectors which add well-characterized epitope tags to the N termini of proteins. The utility of these vectors is demonstrated for detecting the expression of a variety of proteins. As the addition of these epitope tags can in some cases obviate the need to generate specific antisera to each individual protein, these vectors provide a facile means both to monitor protein expression and to purify such expressed proteins.
Sells
M A
MA
Fox Chase Cancer Center, Philadelphia, PA 19111.
Chernoff
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D000939:Epitopes / Q000235:genetics*; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis
D000939:Epitopes; D004247:DNA