Partial sequencing of hog cholera virus Alfort strain genome and its comparison with other pestivirus strains.
After molecular RNA cloning of the Alfort strain (Alfort/LCRV) of hog cholera virus (HCV), the nucleotide sequence of about 70% of the total genome was determined. This sequence was compared with homologous parts of previously published pestivirus genomes. The average homology with another clone of the Alfort strain (Alfort/FRC) was found to be lower (86.1%) than with Brescia strain of HCV (94.3%), while, compared with NADL, Osloss and SD-1 (3 different strains of bovine viral diarrhea virus, BVDV), the average homology was 67%. Although the amino-acid sequences show a higher degree of conservation, they had a similar degree of homology (92.7, 96.7, 69, 68.2 and 69%, respectively). Partial sequence comparison also revealed that Alfort/LCRV strain was more closely related to Alfort 187 (98.6% for the nucleotides and amino acids) and Weybridge (97.3% for the nucleotides and 96.1% for the amino acids) strains of HCV than it was to Alfort/FRC. These results may indicate that the Alfort/FRC strain has undergone more genomic variations during its historical passage. Genomic relationships among the pestiviruses are discussed.
Bakkali Kassimi
L
L
Centre national d'études vétérinaires et alimentaires, laboratoire central de recherches vétérinaires, Maisons-Alfort, France.
Gonzague
M
M
Ermel
G
G
Plateau
E
E
Crucière
C
C
D000818:Animals; D006692:Classical Swine Fever Virus / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010576:Pestivirus / Q000235:genetics*; D012313:RNA / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013552:Swine
D012313:RNA