Trans-activation of the wheat histone H3 promoter by Gal4 DNA-binding domain (1-94) in plant cells.
This is the first demonstration that Gal4 DNA-binding domain (1-94) itself has the ability to trans-activate an eukaryotic promoter in vivo. It significantly activated a wheat histone H3 core promoter, but slightly activated a CaMV 35S core promoter in transiently transfected tobacco cells when the Gal4-recognition sequences were put upstream of the core promoters.
Nakayama
T
T
Department of Botany, Faculty of Science, Kyoto University, Japan.
Ito
T
T
Iwabuchi
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics; D017930:Genes, Reporter; D006657:Histones / Q000235:genetics*; D008969:Molecular Sequence Data; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D029701:Saccharomyces cerevisiae Proteins; D014026:Nicotiana / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation; D014908:Triticum / Q000235:genetics*
D004268:DNA-Binding Proteins; C064255:GAL4 (1-147) protein, S cerevisiae; D006657:Histones; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors