RNA transcription in axotomized dorsal root ganglion neurons.
Levels of RNA transcription were examined in L5 sensory ganglion neurons after unilateral crush injuries of the sciatic nerve using an in situ autoradiographic technique. Neuronal transcription increased in a biphasic pattern on the lesioned side within the first two weeks after injury. The timing and pattern of increases in transcription corresponded to previously reported increases RNA synthesis. The data demonstrate a dynamic regulation of RNA transcription in regenerating neuronal populations. This technique may prove to be useful in the study of factors controlling transcriptional activity in injured neurons.
Wells
M R
MR
Nerve Regeneration Research Laboratory, Department of Veterans Affairs Medical Center, Northport, NY 11768.
Vaidya
U
U
D000818:Animals; D001369:Axons; D005727:Ganglia, Spinal / Q000473:pathology*; D005786:Gene Expression Regulation; D008297:Male; D009409:Nerve Crush; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009475:Neurons, Afferent / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D011920:Rats, Inbred WF; D012584:Sciatic Nerve / Q000293:injuries / Q000502:physiology; D014158:Transcription, Genetic
D009419:Nerve Tissue Proteins; D012313:RNA