Demonstration that thiazole-orange-positive platelets in the dog are less than 24 hours old.
Approximately 6% of dog platelets are positive for staining with thiazole orange, a dye frequently used to stain ribonucleic acid. In this report, thiazole-orange positivity is shown to mark platelets that are less than 24 hours old. Dog platelets were derivatized in vivo with N-hydroxysuccinimido biotin such that greater than 95% of all platelets were biotinylated. Newly synthesized, nonbiotinylated platelets were then monitored by flow cytometry for their ability to bind thiazole orange. After biotinylation, the percentage of biotin-negative, thiazole-orange-positive platelets increased gradually from 0.72% at 30 minutes to 5.44% at 24 hours. These data indicate that thiazole-orange staining does label newly synthesized platelets.
Dale
G L
GL
W.K. Warren Medical Research Institute, Oklahoma City, OK, USA.
Friese
P
P
Hynes
L A
LA
Burstein
S A
SA
D000818:Animals; D052160:Benzothiazoles; D001710:Biotin; D001792:Blood Platelets / Q000737:chemistry / Q000166:cytology* / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D016922:Cellular Senescence; D004285:Dogs / Q000097:blood*; D005434:Flow Cytometry; D011804:Quinolines; D012313:RNA / Q000097:blood; D012503:Saponins / Q000494:pharmacology; D013194:Staining and Labeling; D013844:Thiazoles; D013997:Time Factors
D052160:Benzothiazoles; D011804:Quinolines; D012503:Saponins; D013844:Thiazoles; C051000:thiazole orange; D012313:RNA; D001710:Biotin