Detection of pectinesterase in polyacrylamide gels.
A rapid and sensitive method of detecting pectinesterase activity following electrophoresis or isoelectric focusing in polyacrylamide gels is described. The method uses ruthenium red and requires no addition of substrate when making the gels, thus obviating direct enzyme-substrate contact during electrophoresis. Because of its versatility, the method can be used in a wide variety of applications, such as plant and microbial taxonomy, enzyme purification and characterization, or as an analytical method in fresh and processed plant technology.
Alonso
J
J
Instituto del Frío (CSIC), Madrid.
Rodríguez
M T
MT
Canet
W
W
D002265:Carboxylic Ester Hydrolases / Q000032:analysis*; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007525:Isoelectric Focusing / Q000379:methods*; D010368:Pectins; D010940:Plant Proteins / Q000032:analysis*; D012430:Ruthenium Red / Q000737:chemistry; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D013379:Substrate Specificity; D013997:Time Factors
D010940:Plant Proteins; D012430:Ruthenium Red; D010368:Pectins; D002265:Carboxylic Ester Hydrolases; C022323:pectinesterase