Stability of ascorbic acid, carotenoids, retinol, and tocopherols in plasma stored at -70 degrees C for 4 years.
Aliquots from 40 plasma pools preserved with metaphosphoric acid were assayed for their ascorbic acid values after 12, 24, and 42 months of storage at -70 degrees C. Similarly, aliquots from 16 plasma pools were assayed for values of retinol, several carotenoids, and two tocopherols at 15.5, 27.5, and 51.5 months of storage at -70 degrees C. There were no indications of important losses of these antioxidant micronutrients during storage from the first to the last assay.
Comstock
G W
GW
Training Center for Public Health Research, Hagerstown, Maryland 21742-2067, USA.
Norkus
E P
EP
Hoffman
S C
SC
Xu
M W
MW
Helzlsouer
K J
KJ
D000328:Adult; D000368:Aged; D001205:Ascorbic Acid / Q000097:blood*; D001793:Blood Preservation; D002338:Carotenoids / Q000097:blood*; D015925:Cryopreservation; D004355:Drug Stability; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010949:Plasma / Q000737:chemistry*; D012189:Retrospective Studies; D013997:Time Factors; D014801:Vitamin A / Q000097:blood*; D014810:Vitamin E / Q000097:blood*
D014801:Vitamin A; D014810:Vitamin E; D002338:Carotenoids; D001205:Ascorbic Acid