Ultrastructural study on NOS-immunoreactive nerve terminals in the rat coeliac ganglion.
Nerve terminals immunoreactive for nitric oxide synthase (NOS) were studied in the rat coeliac-superior mesenteric ganglion by electron microscopy using a pre-embedding immunostaining method. The immunoreactive material was distributed in the axoplasmic matrix and was not specifically associated with any subcellular organelle. In most NOS-immunoreactive axon terminals numerous small clear vesicles (35-50 nm) were seen, and in some terminals a small number of large granular vesicles (70-120 nm) were intermingled with small clear vesicles. Most NOS-immunoreactive axon terminals formed axodendritic as well as axo-somatic synapses with non-immunoreactive ganglion neurones, and axo-axonic contacts were very scarce. These results suggest that NO may be released at the synaptic sites from the axon terminals and may affect ganglion neurones.
Domoto
T
T
Department of Anatomy, Shimane Medical University, Izumo, Japan.
Teramoto
M
M
Tamura
K
K
Yasui
Y
Y
D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D005728:Ganglia, Sympathetic / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron; D009411:Nerve Endings / Q000378:metabolism* / Q000648:ultrastructure; D009412:Nerve Fibers / Q000378:metabolism / Q000648:ultrastructure; D019001:Nitric Oxide Synthase; D051381:Rats
D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases