Temperature dependent influence of As2O3, HgHPO4 and KCl on lysosomal acid phosphatase isolated from rat liver.
Studies were carried out to investigate acid phosphatase activity in the presence of As2O3, HgHPO4 and KCl at 25 degrees C and 37 degrees C. In all cases examined enzyme activities measured at 25 degrees differ from those detected at 37 degrees C. When activity was measured in the presence of As2O3 at 25 degrees C a stimulation was found while at 37 degrees C activities remained within the control range. Similar results were obtained, when As2O3 was replaced by HgHPO4. In contrast to that, added amounts of KCl cause an increase of activity at both incubation temperatures, but the increment being greater at 37 degrees C. Furthermore in most cases correlation between increasing amounts of substances added and enzyme activity measured was non-linear.
Dittmann
J
J
Institut für Physiologische Chemie, Tierärztliche Hochschule Hannover, Germany.
Harisch
G
G
D000135:Acid Phosphatase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D017669:Mercury Compounds / Q000494:pharmacology*; D010087:Oxides / Q000494:pharmacology*; D010710:Phosphates / Q000494:pharmacology*; D011189:Potassium Chloride / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013816:Thermodynamics
D001152:Arsenicals; D017669:Mercury Compounds; D010087:Oxides; D010710:Phosphates; D011189:Potassium Chloride; C078234:mercury phosphate; D000135:Acid Phosphatase; D000077237:Arsenic Trioxide