[Effect of phorbol ester on the proliferation and differentiation of blast cells from leukemia patients and blast secretion of biologically active products].
The influence of tetra phorbol diesther (TPA) on primary blast cells of patients with acute leukemia and blastic crises of chronic myelogenous leukemia and the influence of the condition medium (CM) of the primary and TPA-treated blast cells on the proliferation of HL-60 cell line has been studied. The level of interferon-alpha (IFN-alpha) in CM was tested. TPA inhibited proliferation and induced macrophage-like differentiation of primary AML blast cells and these changes were accompanied by modulation of IFN-alpha expression in CM. The effect of blast CM on proliferation of HL-60 was both inhibitory and stimulating and depended on the time of treatment and individual characteristics of patients. It has been shown that the level of IFN-alpha in CM was not correlated with antiproliferative effect of CM. The role of individual differences in capability of primary blast cells to be induced by differentiated agents and the nature of these differences are discussed.
Machiulaĭtene
E R
ER
Blagosklonnyĭ
M V
MV
Lasunskaia
E B
EB
D001752:Blast Crisis / Q000473:pathology*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000187:drug effects*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D013755:Tetradecanoylphorbol Acetate