Sequence-based typing of HLA-A2 alleles using a primer with an extra base mismatch.
A PCR-SBT method using genomic DNA for HLA-A2 alleles was established. To achieve specific amplification for detecting a single base difference between A2 alleles and other HLA-A alleles, a primer having one extra mismatch at the second position from its 3'-end was designed. The primer exhibited a high specificity with annealing temperatures from 64 degrees C to 68 degrees C. Thirty-eight Japanese samples were screened using this method. The majority of Japanese A2 antigens were coded by A*0201. A*0206 and A*0207 were observed at relatively high frequencies. Serologically defined split antigens, A2S and A2AK, which we have recently identified, corresponded to A*0203 and A*0210, respectively. Moreover, A*0207 was strongly associated with B46 and DR8.1.
Ishikawa
Y
Y
Department of Research, Japanese Red Cross Central Blood Center, Tokyo.
Tokunaga
K
K
Kashiwase
K
K
Akaza
T
T
Tadokoro
K
K
Juji
T
T
D000483:Alleles; D001482:Base Composition; D001483:Base Sequence; D003601:Cytotoxicity Tests, Immunologic; D017931:DNA Primers / Q000235:genetics*; D015789:HLA-A2 Antigen / Q000235:genetics* / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D015789:HLA-A2 Antigen