DNA content and alkaline phosphatase expression in cells of different gingival overgrowths.
The present study compared the alkaline phosphatase (ALPase) expression and DNA content at specific periods in cultured cells derived from non-inflamed enlarged gingivae of idiopathic gingivofibromatosis (IGF) and phenytoin-induced hyperplasia (PHG). Cultured cells from healthy gingiva or periodontal ligament (PDL) were used as controls. The DNA assay, ALPase assay and cytochemical staining for ALPase in cultured cells were performed at four, seven, and nine days. The presence of intense ALPase activity was a prominent feature in cultured IGF cells, whereas very low ALPase activity was detected in PHG cells. The cell lines tested showed no significant differences in DNA content. The expression of ALPase in these cells was population density-dependent. The observation that cells isolated from both types of gingival overgrowth exhibited a different ALPase profile at variance with normal gingival fibroblasts suggested that a distinct pathogenic mechanism may be involved in each type of gingival overgrowth.
Hou
L T
LT
Department of Periodontology, School of Dentistry, National Taiwan University, Taipei, ROC.
Yaeger
J A
JA
D000469:Alkaline Phosphatase / Q000032:analysis / Q000235:genetics*; D002452:Cell Count; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism; D005351:Fibromatosis, Gingival / Q000201:enzymology* / Q000235:genetics*; D005434:Flow Cytometry; D015971:Gene Expression Regulation, Enzymologic; D005881:Gingiva / Q000201:enzymology / Q000378:metabolism; D005885:Gingival Hyperplasia / Q000139:chemically induced / Q000201:enzymology* / Q000235:genetics*; D006801:Humans; D010513:Periodontal Ligament / Q000201:enzymology / Q000378:metabolism; D010672:Phenytoin / Q000009:adverse effects; D013194:Staining and Labeling
D010672:Phenytoin; D004247:DNA; D000469:Alkaline Phosphatase