title: Human foamy virus infection activates class I major histocompatibility complex antigen expression.
abstract: We examined the effect of human foamy virus (HFV) infection on the expression of human major histocompatibility complex molecules. Our data show that in vitro HFV infection of U373-MG glioblastoma cells results in increased expression of class I human leukocyte antigen (HLA) and transcripts. Transient transfection assays of plasmids containing the reporter gene chloramphenicol acetyl transferase driven by different 5' deletions of the HLA-A11 class I promoter allowed identification of cis-acting elements involved in this regulation. HFV infection has two opposite effects on the HLA class I promoter: transactivation of the HLA-A11 promoter through a positive regulatory element located in the -525 to -335 region upstream of exon 1 and down-regulation of transcriptional activity driven by the -335 to -205 class I promoter region. Additional experimental data indicate that the effect of HFV on HLA class I expression is not mediated by the interferon pathway.
authors.affiliation: UPR A0043 CNRS, Hôpital St Louis, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Colas
authors.forename: J F
authors.initials: JF
authors.lastname: Bourge
authors.forename: J
authors.initials: J
authors.lastname: Wybier
authors.forename: M K
authors.initials: MK
authors.lastname: Chelbi-Alix
authors.forename: P
authors.initials: P
authors.lastname: Paul
authors.forename: R
authors.initials: R
authors.lastname: Emanoil-Ravier
mesh_terms: D000227:Adenine Nucleotides; D001483:Base Sequence; D015536:Down-Regulation; D005786:Gene Expression Regulation; D005805:Genes, MHC Class I / Q000235:genetics*; D005802:Genes, MHC Class II / Q000235:genetics; D006684:HLA-DR Antigens / Q000096:biosynthesis; D006801:Humans; D007372:Interferons / Q000032:analysis; D008025:Ligases / Q000378:metabolism; D008969:Molecular Sequence Data; D009843:Oligoribonucleotides; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D017384:Sequence Deletion / Q000502:physiology; D016092:Spumavirus / Q000502:physiology*; D015533:Transcriptional Activation; D014407:Tumor Cells, Cultured
chemical_list: D000227:Adenine Nucleotides; D006684:HLA-DR Antigens; D009843:Oligoribonucleotides; D012333:RNA, Messenger; C023505:2',5'-oligoadenylate; D007372:Interferons; D008025:Ligases