Topographic changes in a heterochromatic chromosome block in humans (15P) during formation of the nucleolus.
Fluorescence in situ hybridization and multispectral confocal laser scanning microscopy revealed a highly dynamic nucleolar-associated chromosome 15 satellite III heterochromatin cluster in humans. This nucleolar-associated DNA was highly decondensed at the metaphase plate compared with its topography at interphase and appeared to act as a centre for the post-mitotic reorganization of the nucleolus. Our data showed unexpected trans-mitotic changes in the topography of this nucleolar-associated satellite III DNA that suggest that this locus-specific heterochromatin superstructure may be involved in nucleolar organization.
Kaplan
F S
FS
Department of Orthopaedics, Hospital of the University of Pennsylvania, Philadelphia 19104, USA.
O'Connor
J P
JP
D002466:Cell Nucleolus / Q000648:ultrastructure*; D002884:Chromosomes, Human, Pair 15; D004276:DNA, Satellite; D006367:HeLa Cells; D006570:Heterochromatin / Q000235:genetics / Q000648:ultrastructure*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018613:Microscopy, Confocal
D004276:DNA, Satellite; D006570:Heterochromatin