Expression of glycine receptor subunits and gephyrin in single bipolar cells of the rat retina.
We studied the expression of glycine receptor (GlyR) subunits and gephyrin in the adult rat retina. Reverse transcribed RNA was amplified by polymerase chain reaction (RT-PCR) with primers designed to recognize GlyR alpha 1, alpha 2, alpha 3, beta subunits, and gephyrin. Using RNA isolated from the whole retina, signals for all four GlyR subunits and gephyrin could be observed. In rod bipolar cells, in contrast, we detected a subset of GlyR subunits, alpha 1 and beta, and no gephyrin. Patch-clamp recording employing two subtype-specific blockers of the GlyR, picrotoxinin and cyanotriphenylborate (CTB), indicated that the GlyR in rod bipolar cells is a heteromeric protein composed of the alpha 1 and beta subunit. Moreover, the absence of detectable amounts of gephyrin mRNA suggests that the anchor protein is not required for the function of GlyRs in rod bipolar cells.
Enz
R
R
Max-Planck-Institut für Hirnforschung, Frankfurt, Germany.
Bormann
J
J
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001881:Borates / Q000494:pharmacology; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D004594:Electrophysiology; D007395:Interneurons / Q000502:physiology*; D008564:Membrane Potentials; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D010852:Picrotoxin / Q000031:analogs & derivatives / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D012313:RNA / Q000302:isolation & purification; D051381:Rats; D017208:Rats, Wistar; D018009:Receptors, Glycine / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000235:genetics; D012160:Retina / Q000166:cytology* / Q000378:metabolism; D054830:Sesterterpenes; D014158:Transcription, Genetic
D001881:Borates; D002352:Carrier Proteins; D017931:DNA Primers; D008565:Membrane Proteins; D010852:Picrotoxin; D012313:RNA; D018009:Receptors, Glycine; D054830:Sesterterpenes; C073441:gephyrin; C089415:cyanotriphenylborate; C022961:picrotoxinin