Molecular cloning and sequencing of the gene encoding a sheep arginine vasopressin type 1a receptor.
The gene for the sheep arginine vasopressin type 1a (V1a) receptor subtype was cloned from a genomic library. The deduced amino acid sequence shows characteristics of a G-protein coupled receptor and high sequence identity to human and rat V1a receptor sequences (81% and 73%, respectively). Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed a tissue distribution consistent with a type V1a receptor. The genomic DNA (7.1 kb) contains a 1586 bp intron between the putative 6th and 7th transmembrane domains.
Hutchins
A M
AM
Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia.
Phillips
P A
PA
Venter
D J
DJ
Burrell
L M
LM
Johnston
C I
CI
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D015698:Genomic Library; D008969:Molecular Sequence Data; D017483:Receptors, Vasopressin / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012756:Sheep
D017483:Receptors, Vasopressin; D004247:DNA