[Effects of scopolamine on serotonin in the spinal cord and atria of morphine-dependent rats].
The effects of scopolamine on serotonin (5-HT) in the spinal cord and atria of morphine-dependent rats were studied. Levels of 5-HT and its metabolite, 5-hydroxyindolactic acid (5-HIAA) in the spinal cord and atria were analyzed by HPLC-ECD method. The results showed that contents of 5-HT, 5-HIAA in the spinal cord of morphine-dependent rats were statistically higher than those of controls (P < 0.01, n = 6), and that scopolamine inhibited morphine's effect by decreasing 5-HT, 5-HIAA in the spinal cord. After acute abstinence (1d), the contents of 5-HT in the spinal cord in natural withdrawal group were decreased, but increased in scopolamine group. After chronic abstinence (6d), the contents of 5-HT in the spinal cords in the two groups were all increased to above normal level. The results also showed that scopolamine did not affect the increasing of 5-HT in atria induced by morphine. The data support a role of muscarinic receptor on changes of 5-HT metabolism in the spinal cord in morphine-dependent rats.
Zhang
F
F
Ningbo Institute of Microcirculation and Henbane.
Zhou
W
W
Chen
Q
Q
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006325:Heart Atria / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism*; D009021:Morphine Dependence / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012601:Scopolamine / Q000494:pharmacology*; D012701:Serotonin / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*
D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D012601:Scopolamine