Detection of antibodies to L1, L2, and E4 proteins of human papillomavirus types 6, 11, and 16 by ELISA using synthetic peptides.
Antibodies against eight synthetic peptides spanning different epitopes located on L1, L2, and E4 proteins of human papillomavirus (HPV) types 16, 6, and 11 were examined in sera from 73 women infected by HPV and from 139 healthy controls. Only three of these peptides were reactive. Two located on proteins L2 and E4 of HPV 16 seem type specific since antibodies to these peptides were detected, respectively, in 21% and 15% of the HPV 16 infected patients and in 2.5% and none of women infected by other HPVs. The third peptide located on the L1 protein of HPV 6 bears a common epitope since antibodies to this peptide were detected not only in 85% of women infected by HPV 6 or 11, but also in 82% of women infected by other HPVs, and in 74% and 71% of the control groups (10-12-year-old children and adults, respectively). In conclusion, none of the peptides investigated seems useful to develop ELISAs for serological diagnosis of HPV infection.
Le Cann
P
P
Institut de Virologie, Faculté de Pharmacie, Tours, France.
Chabaud
M
M
Leboulleux
D
D
Mougin
C
C
Mayelo
V
V
Legrand
M C
MC
Calvet
C
C
Afoutou
J M
JM
Coll-Seck
A M
AM
Coursaget
P
P
D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification; D002213:Capsid / Q000276:immunology / Q000302:isolation & purification; D036022:Capsid Proteins; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000276:immunology / Q000302:isolation & purification*; D027383:Papillomaviridae / Q000145:classification / Q000276:immunology* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000276:immunology; D015995:Prevalence; D011505:Protein-Tyrosine Kinases / Q000276:immunology / Q000302:isolation & purification; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D014764:Viral Proteins
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; C063316:L2 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D010446:Peptide Fragments; D014764:Viral Proteins; C069119:oncogene protein E4, Human papillomavirus type 16; C067860:oncogene protein E4, type 11, Human papillomavirus; C091560:L1 protein, Human papillomavirus type 6; C063317:L1 protein, Human papillomavirus type 16; D011505:Protein-Tyrosine Kinases