Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo.
Human interferon-inducible protein 10 (IP-10), a member of the alpha chemokine family, inhibits bone marrow colony formation, has antitumor activity in vivo, is chemoattractant for human monocytes and T cells, and promotes T cell adhesion to endothelial cells. Here we report that IP-10 is a potent inhibitor of angiogenesis in vivo. IP-10 profoundly inhibited basic fibroblast growth factor-induced neovascularization of Matrigel (prepared by H. K. Kleinman) injected subcutaneously into athymic mice. In addition, IP-10, in a dose-dependent fashion, suppressed endothelial cell differentiation into tubular capillary structures in vitro. IP-10 had no effect on endothelial cell growth, attachment, and migration as assayed in vitro. These results document an important biological property of IP-10 and raise the possibility that IP-10 may participate in the regulation of angiogenesis during inflammation and tumorigenesis.
Angiolillo
A L
AL
Department of Hematology/Oncology, Children's National Medical Center, Washington, DC 20010, USA.
Sgadari
C
C
Taub
D D
DD
Liao
F
F
Farber
J M
JM
Maheshwari
S
S
Kleinman
H K
HK
Reaman
G H
GH
Tosato
G
G
D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D003094:Collagen; D016207:Cytokines / Q000494:pharmacology* / Q000627:therapeutic use; D004338:Drug Combinations; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000037:antagonists & inhibitors / Q000633:toxicity; D006801:Humans; D007797:Laminin; D008168:Lung / Q000098:blood supply; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008969:Molecular Sequence Data; D009389:Neovascularization, Pathologic / Q000139:chemically induced / Q000517:prevention & control*; D011509:Proteoglycans; D014471:Umbilical Veins
D054357:Chemokine CXCL10; D019743:Chemokines, CXC; D016207:Cytokines; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D016222:Fibroblast Growth Factor 2; C059485:matrigel; D003094:Collagen