Different T-cell activation by streptozotocin and Freund's adjuvant in popliteal lymph node (PLN).
A popliteal lymph node (PLN) test was further validated for predictive screening of autoimmunity-inducing drugs. Autoimmune-like T-cell activation of streptozotocin (STZ) was compared with the effect of Freund's complete adjuvant (FCA), injected locally into the foot pad of BALB/c mice. Early cell activation in enlarged PLN was monitored by flow cytometry. Injection of both STZ and FCA markedly increased the absolute PLN cell number as well as specific T-helper (CD4+), T-suppressor/cytotoxic (CD8+), and B (Ig+) subsets. However, quantitative analysis of early T-cell activation revealed important differences between STZ-induced PLN reaction and FCA-related lymphoproliferation. At 72 h, the number of cells stained with anti-early activation marker (EAM+; CD69+) increased over 10 times in STZ-enlarged nodes and only 3 times in the FCA-inflamed nodes. Furthermore, different cytometric profiles were noted for STZ-activated and FCA-activated cells stained with anti-interleukin-2 receptor (IL-2R) (CD25+). The data suggest the applicability of early cytometric screening of enlarged PLN for predictive analysis detection of chemicals inducing an autoimmune-like reaction.
Krzystyniak
K
K
Département des Sciences Biologiques, Université de Québec, Montréal, Canada.
Kozlowska
E
E
Desjardins
R
R
Drela
N
N
Kowalczyk
R
R
Karwowska
K
K
Izdebska-Szymona
K
K
D000818:Animals; D001327:Autoimmune Diseases / Q000209:etiology / Q000276:immunology; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology*; D008198:Lymph Nodes / Q000187:drug effects* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D009929:Organ Size / Q000187:drug effects; D010672:Phenytoin / Q000494:pharmacology; D011342:Procainamide / Q000494:pharmacology; D013311:Streptozocin / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D013311:Streptozocin; D010672:Phenytoin; D005620:Freund's Adjuvant; D011342:Procainamide