Recovery of DNA, RNA and protein from gels with microconcentrators.
The use of a new product, Microcon/Micropure (a centrifugal ultrafiltration device combined with a microporus insert), for the purification of DNA, RNA, peptides and proteins from gels is described. Using this system, DNA can be recovered from agarose gel in concentrated, contamination-free form in only 15 min. Results of studies on the effects of fragment size and various pretreatment of the gel slice on DNA recovery are presented. The Microcan/Micropure combination can also be used for the recovery of macromolecules from polyacrylamide gels. Optimized protocols for the recovery of RNA, oligonucleotides and proteins from polyacrylamide gels using a crush and elute method, along with a study of critical parameters, are presented.
Krowczynska
A M
AM
Amicon, Inc., Beverly, MA, USA.
Donoghue
K
K
Hughes
L
L
D002498:Centrifugation / Q000379:methods; D004247:DNA / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005615:Freezing; D005782:Gels / Q000737:chemistry*; D009841:Oligonucleotides / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012313:RNA / Q000302:isolation & purification*; D013997:Time Factors; D014462:Ultrafiltration / Q000295:instrumentation*
D005782:Gels; D009841:Oligonucleotides; D010455:Peptides; D011506:Proteins; D012313:RNA; D004247:DNA