Chimeric alpha-beta oligonucleotides as antisense inhibitors of reverse transcription.
Alpha-beta chimeric 17-mer oligodeoxyribonucleotides containing either 5, 10 or 15 beta nucleotides were synthesized. The stability of the RNA/chimera hybrids was only slightly affected by the alpha stretch and by the alpha-beta link, as was the affinity of the Moloney Murine Leukemia Virus reverse transcriptase for the duplexes. All chimeras inhibited in vitro cDNA synthesis in a cell-free system to various extent, via the degradation of the RNA target by RNase H.
Boiziau
C
C
Laboratoire de Biophysique Moléculaire, INSERM U 386, Université Bordeaux II, France.
Debart
F
F
Rayner
B
B
Imbach
J L
JL
Toulme
J J
JJ
D001483:Base Sequence; D016373:DNA, Antisense / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D018076:DNA, Complementary / Q000096:biosynthesis; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000201:enzymology; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D018894:Reverse Transcriptase Inhibitors; D016914:Ribonuclease H; D014158:Transcription, Genetic / Q000187:drug effects*
D016373:DNA, Antisense; D018076:DNA, Complementary; D009838:Oligodeoxyribonucleotides; D011089:Polydeoxyribonucleotides; D018894:Reverse Transcriptase Inhibitors; D016914:Ribonuclease H