Quantification of a major bovine allergen by a two-site immunometric assay based on monoclonal antibodies.
A two-site immunometric assay based on monoclonal antibodies (mAb) was developed for the measurement of a 20-kDa major allergen of cow. The sensitivity of this assay was (BDA20) 1 ng/ml. It was used to measure airborne allergen concentrations in 10 Finnish cowsheds. The mean concentration of the BDA20 measured at two stationary sites was 280 ng/m3. Concentrations varied more than 10-fold among cowsheds (54-804 ng/m3). The mean intertest coefficient of variation was 8.2%, and the mean intratest variation 4.1%.
Ylönen
J
J
Department of Clinical Microbiology, University of Kuopio, Finland.
Virtanen
T
T
Rytkönen
M
M
Mäntyjärvi
R
R
D016902:Air Pollution, Indoor / Q000032:analysis; D000485:Allergens / Q000032:analysis / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions / Q000276:immunology*; D002415:Cats; D002417:Cattle; D003429:Cross Reactions; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D006736:Horses; D007114:Immunization / Q000379:methods; D051379:Mice; D008807:Mice, Inbred BALB C; D011485:Protein Binding; D012680:Sensitivity and Specificity
D000485:Allergens; D000911:Antibodies, Monoclonal; D000939:Epitopes