Effect of hexachlorocyclohexane (HCH) on testicular plasma membrane of rat.
The study deals with the analysis of residue of hexachlorocyclohexane (HCH) and its possible damaging potential on testicular plasma membrane of rats. In vitro studies were conducted by exposing plasma membrane of testis with 1.46 x 10(-5) M, 2.92 x 10(-5) M, 4.38 x 10(-5) M, 7.30 x 10(-5) M, 1.46 x 10(-4) M and 2.92 x 10(-4) M HCH at 37 degrees C for 15 min. The results indicate inhibition in the activity of the Ca(2+)-ATPase, Na(+)+K(+)+Mg(2+)-ATPase and 5'Nucleotidase. In vivo studies were carried out following repeated dermal exposure to HCH at a dose level of 50 or 100 mg/kg/day for 60 days to male rats. The results show significant decrease in the activities of 5'-Nucleotidase, Ca(2+)-ATPase, Na(+) + K(+)-ATPase and Mg(2+)-ATPase in the plasma membrane of testis following exposure to HCH. The analysis of the residues of HCH reveals the presence of significant quantities of its different isomers viz., alpha, beta, gamma and delta in the testicular plasma membrane of rats given in vivo dermal exposure of this pesticide. These results suggest that the presence of HCH residue may be a factor in inhibiting the marker enzymes of the plasma membrane of testis.
Srivastava
S C
SC
Industrial Toxicology Research Centre, Lucknow, India.
Kumar
R
R
Prasad
A K
AK
Srivastava
S P
SP
D015720:5'-Nucleotidase / Q000037:antagonists & inhibitors; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D013737:Testis / Q000187:drug effects* / Q000201:enzymology
D001556:Hexachlorocyclohexane; D015720:5'-Nucleotidase; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase