A novel T-cell differentiation antigen expressed in immature human thymocytes and neuroglial cells.
We report a novel human thymocyte differentiation antigen ICT-1 with a molecular weight of 49 kDa that is noncovalently associated with another 12-kDa protein. The ICT-1 antigen is expressed in 50-70% of total thymocytes, but not in resting or PHA-activated peripheral blood T-cells and bone marrow cells. The thymocytes expressing ICT-1 antigen appear after the 18th week of gestation during fetal development. Since the distribution pattern of the ICT-1 antigen within thymus partly overlaps with that of the CD1 antigens, we investigated whether ICT-1 was one of the CD1 antigen family. However, the failure of anti-ICT-1 antibody to react with mouse L cells transfected with cDNA of CD1a, -b, and -c and the different histologic distribution patterns from that of CD1d strongly suggest that the anti-ICT-1 antibody recognizes an antigen distinct from CD1. Furthermore, ICT-1 is also expressed in human neuroglial cells such as oligodendroglioma, glioblastoma multiforme, Ewing's sarcoma, and cerebellar astrocyte. Hence we believe that the ICT-1 antigen may be a novel thymus-leukemia (TL) antigen or a nonclassical MHC class I antigen.
Bae
Y M
YM
Department of Pathology, Seoul National University College of Medicine, Korea.
Kim
T J
TJ
Park
W S
WS
Chung
D H
DH
Choi
E Y
EY
Lee
G K
GK
Song
H G
HG
Kim
C W
CW
Kim
B K
BK
Ahn
H S
HS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000096:biosynthesis* / Q000276:immunology; D018949:Antigens, CD1; D000945:Antigens, Differentiation, T-Lymphocyte / Q000737:chemistry / Q000502:physiology*; D005314:Embryonic and Fetal Development / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013950:Thymus Gland / Q000166:cytology* / Q000378:metabolism*; D014162:Transfection / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018949:Antigens, CD1; D000945:Antigens, Differentiation, T-Lymphocyte; D008562:Membrane Glycoproteins; D010835:Phytohemagglutinins; C028388:thymus-leukemia antigens