Recent advances in differential display.
Differential display and RNA arbitrary primed polmerase chain reaction are methods recently designed to identify and isolate differentially expressed genes. Methodological modifications have since been introduced to streamline the techniques. The major effort has centered on how to eliminate false positives as approached from a variety of angles, ranging from RNA sample preparation, northern blot confirmation, primer length variation, to better experimental design.
Liang
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Division of Cell Growth and Regulation, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
Pardee
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D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000639:trends