Myosin-V is present in synaptosomes from rat cerebral cortex.
The subcellular localization in brain of an unconventional, calmodulin-binding myosin (myosin-V) found in neurons, astrocytes and other secretory cells of vertebrates has been investigated by probing Western blots of synaptic fractions from rat cerebral cortex with affinity-purified polyclonal antibodies against myosin-V. Myosin-V was detected in intact synaptosomes and in lysed synaptosomes associated with a particulate fraction. Our data suggest a role for brain myosin-V in membrane-cytoskeleton function in the synaptic region.
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Departamento de Bioquímica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil.
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Larson
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D000818:Animals; D002148:Calmodulin-Binding Proteins / Q000032:analysis*; D002540:Cerebral Cortex / Q000737:chemistry*; D024701:Myosin Type V; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051381:Rats; D013574:Synaptosomes / Q000737:chemistry*
D002148:Calmodulin-Binding Proteins; D009419:Nerve Tissue Proteins; C078829:chicken brain myosin-V p190; D024701:Myosin Type V