Role of rat liver plasma membrane phospholipids in regulation of protein kinase activities.
The role of rat liver plasma membrane phospholipids in the regulation of protein kinase A, protein kinase C and tyrosine kinase activities was investigated. Plasma membrane composition was modified by phospholipase A2, phospholipase C and phospholipase D treatment and subsequent incorporation of various phospholipids. Phospholipase A2 deactivated the three types of protein kinases, while phospholipase C and D affected the enzymes in a different manner. Phosphatidylcholine and sphingomyelin were found to be the most effective activators of protein kinase A and tyrosine kinase. Incorporation of sphingomyelin and phosphatidylserine into partially delipidated plasma membranes resulted in a significant stimulation of protein kinase C activity. Since sphingomyelin appeared to be a specific effector of the three types of protein kinases under investigation, one might suggest that its role in cellular signaling could be manifested via regulation of protein kinase C as well as via modulation of protein kinase A and tyrosine kinase activities.
Gavrilova
N J
NJ
Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.
Petkova
D H
DH
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D010739:Phospholipase D / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D010738:Type C Phospholipases / Q000378:metabolism
D008563:Membrane Lipids; D010743:Phospholipids; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases; D010739:Phospholipase D