title: Localization of a new ferritin heavy chain sequence present in human brain mRNA to chromosome 11.
abstract: Two types of ferritin heavy (H) chain clones have been isolated from cDNA libraries of human fetal and adult brain: one corresponds to the ferritin H chain mRNA that is abundant in liver and is called "liver-like" brain cDNA; the other contains an additional 279 nucleotide (nt) sequence in the 3' untranslated region and is called brain ferritin H chain cDNA. To map the 279-nt sequence, polymerase chain reaction (PCR) amplification was carried out using DNA from rodent x human hybrid cell lines containing single human chromosomes as templates, and oligomeric primers homologous to the 3' end of the 279-nt sequence (primer A) and to a coding sequence just 5' to the 279-nt sequence. Significant PCR product of the size expected from analysis of the brain ferritin H chain cDNA clones and a genomic ferritin H chain clone (487 bp) was generated only from hybrid-cell DNA containing human chromosome 11. This PCR product and the "liver-like" brain cDNA (lacking the 279-nt sequence) both hybridized to chromosome 11 fragments that are known to define the well-characterized functional liver ferritin H chain gene and a putative pseudogene. Preliminary data indicate that primer A (and thus the 279-nt sequence) maps to the functional ferritin H chain gene fragments, but binding to the pseudogene has not been ruled out.
authors.affiliation: Division of Biomedical Services and Research, Surrey Place Centre, Toronto, ON, Canada.
authors.forename: M E
authors.initials: ME
authors.lastname: Percy
authors.forename: S J
authors.initials: SJ
authors.lastname: Bauer
authors.forename: S
authors.initials: S
authors.lastname: Rainey
authors.forename: D R
authors.initials: DR
authors.lastname: McLachlan
authors.forename: M S
authors.initials: MS
authors.lastname: Dhar
authors.forename: J G
authors.initials: JG
authors.lastname: Joshi
mesh_terms: D000328:Adult; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern / Q000379:methods; D001923:Brain Chemistry; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific; D005293:Ferritins / Q000737:chemistry* / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D012333:RNA, Messenger; D004247:DNA; D005293:Ferritins; D015246:Deoxyribonuclease EcoRI; D015247:Deoxyribonuclease HindIII; D015252:Deoxyribonucleases, Type II Site-Specific