A highly sensitive enzyme immunoassay for G-CSF in human plasma.
We have developed a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) to measure granulocyte colony-stimulating factor (G-CSF) in human plasma. This ELISA employs a combination of a mouse monoclonal antibody (MAb) as the first antibody and an affinity-purified sheep polyclonal antibody conjugated with beta-D-galactosidase as the second antibody. The coefficients of intra- and interassay variations were 2.2 to 3.6% and 8.3 to 10.2%, respectively. The assay had no cross-reactivity with four other human cytokines. Plasma G-CSF levels in healthy volunteers could easily be measured because the detection limit was 0.5 pg/mL. The mean plasma G-CSF concentration in 57 healthy volunteers (aged 19 to 47; 27 males and 30 females) was 10.5 +/- 4.5 pg/mL standard deviation [SD]). There was no statistically significant relationship between plasma G-CSF concentration and absolute neutrophil counts in those healthy volunteers (r = 0.259; p = 0.052).
Ichikawa
T
T
Pharmaceutical Development Laboratory, Kirin Brewery Co., Ltd., Japan.
Kuwaki
T
T
Tachibana
K
K
Yanagida
M
M
Matsuki
S
S
D000328:Adult; D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012756:Sheep
D016179:Granulocyte Colony-Stimulating Factor