Structure and chromosomal localization of a human water channel (AQP3) gene.
A cDNA encoding rat AQP3, a water channel and a member of the MIP family, that is expressed predominantly in kidney medulla and colon was cloned recently. To determine the structure, tissue distribution, and chromosomal localization of the human AQP3 gene, we screened a human kidney cDNA library with rat AQP3 probe and isolated a cDNA coding for human AQP3 protein. The deduced amino acid sequence of human AQP3 was 91% identical to rat AQP3. Human AQP3 mRNA was expressed in colon, kidney, liver, pancreas, lung, peripheral leukocytes, spleen, and prostate. The human AQP3 gene was mapped to 7q36.2-q36.3 by chromosome fluorescence in situ hybridization.
Ishibashi
K
K
Second Department of Internal Medicine, School of Medicine, Tokyo, Japan.
Sasaki
S
S
Saito
F
F
Ikeuchi
T
T
Marumo
F
F
D000595:Amino Acid Sequence; D000818:Animals; D051400:Aquaporin 3; D020346:Aquaporins; D001483:Base Sequence; D002874:Chromosome Mapping; D002897:Chromosomes, Human, Pair 7; D018076:DNA, Complementary / Q000737:chemistry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007473:Ion Channels / Q000235:genetics*; D008969:Molecular Sequence Data; D051381:Rats
C496027:AQP3 protein, human; C496029:Aqp3 protein, rat; D020346:Aquaporins; D018076:DNA, Complementary; D007473:Ion Channels; D051400:Aquaporin 3