Fetuin is a substrate for Pasteurella haemolytica O-sialoglycoprotease.
A soluble bovine glycoprotein, fetuin, was used as an alternative substrate to identify O-sialoglycoprotease activity in culture supernatant protein fractions of Pasteurella haemolytica. An aliquot of a 24-hour incubation mixture containing fetuin and O-sialoglycoprotease was denatured and examined after gradient sodium dodecyl polyacrylamide gel electrophoresis. The Coomassie-Brilliant-Blue-stained gel was examined for the disappearance of the fetuin band at an apparent molecular mass of 64 KDa. Four major hydrolysis products were identified: an N-terminal fragment of 45 kDa, a 20 kDa fragment at Val50, and two C-terminal fragments at Val273 and His287.
Tabatabai
L B
LB
National Animal Disease Center, USDA, ARS, Ames, Iowa 50010, USA.
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D002413:Cations, Divalent / Q000494:pharmacology; D002417:Cattle; D006868:Hydrolysis; D066298:In Vitro Techniques; D007700:Kinetics; D016978:Mannheimia haemolytica / Q000201:enzymology*; D008666:Metalloendopeptidases / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D000509:alpha-Fetoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D002413:Cations, Divalent; D010446:Peptide Fragments; D000509:alpha-Fetoproteins; D008666:Metalloendopeptidases; C070738:O-sialoglycoprotein endopeptidase