Keratin expression in cutaneous lichen planus.
The characteristic expression of keratins by keratinocytes is well documented. A typical 'hyperproliferative' profile of epidermal keratin expression occurs in psoriasis, wound healing and warts. This study analyses keratin expression in cutaneous lichen planus to determine abnormalities of differentiation occurring in this inflammatory disorder. Using a panel of monoclonal antibodies 28 samples (20 patients) were studied. The results showed that squamous differentiation was unaffected, with keratins K1 and K10 being expressed normally for the site sampled. The main abnormalities included extension of reactivity of the basal cell marker, LH8, into the suprabasal compartment. Keratin K17, usually restricted to adnexal structures, was variably expressed in the basal and suprabasal layers of the interfollicular epithelium of affected epidermis. Keratins K6 and K16, found suprabasally in hyperproliferative states, were detected both basally and suprabasally in all diseased samples. The keratin profile in lichen planus is analogous to the wound healing response. Suprabasal keratin K17 is found in psoriasis, wound healing and viral warts so the changes in keratin K17 may reflect hyperproliferative changes. It is likely that the changes in epidermal keratin expression are due to up-regulation of specific keratin genes by the production of cytokines and inflammatory mediators from the lymphocytic infiltrate typical of lichen planus.
Schofield
J K
JK
Department of Experimental Dermatology, London Hospital Medical College, UK.
De Berker
D
D
Milligan
A
A
Rohloff
W
W
Marren
P
P
Davies
M G
MG
Burge
S
S
Graham-Brown
R
R
Leigh
I M
IM
D000911:Antibodies, Monoclonal / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000096:biosynthesis* / Q000276:immunology; D008010:Lichen Planus / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007633:Keratins