title: The temporal profiles of ICAM-1 protein and mRNA expression after transient MCA occlusion in the rat.
abstract: Leukocytes may contribute to ischemic cell damage. ICAM-1 expression on endothelial cells facilitates the migration of leukocytes into tissue. Therefore, we measured the temporal profiles of ICAM-1 mRNA and protein in rat brain after transient (1 or 2 h) of middle cerebral artery (MCA) occlusion. Male Wistar rats (n = 86) were subjected to 1 or 2 h MCA of occlusion, or 2 h of MCA occlusion followed by reperfusion for a variety of durations ranging from 1 h to 1 week. 10 additional control animals were employed. ICAM-1 mRNA and protein were measured during ischemia and reperfusion, and immunohistochemical methods were used to identify specific cell types expressing ICAM-1. ICAM-1 mRNA was detected 1 h after the onset of ischemia. mRNA maximized at 10 h of reperfusion and persisted out to 1 week of reperfusion. ICAM-1 significantly increased in microvascular endothelial cells at 2 h of reperfusion, maximized at 46 h and persisted out to 1 week of reperfusion (P < 0.05). ICAM-1 mRNA and protein are present in ischemic brain early after the onset of ischemia and reperfusion, respectively. These data provide support for the role of ICAM-1 in mediating leukocyte-endothelial adhesion after transient MCA occlusion in the rat.
authors.affiliation: Department of Neurology, Henry Ford Hospital, Detroit, MI 48202, USA.
authors.forename: R L
authors.initials: RL
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Chopp
authors.forename: C
authors.initials: C
authors.lastname: Zaloga
authors.forename: Z G
authors.initials: ZG
authors.lastname: Zhang
authors.forename: N
authors.initials: N
authors.lastname: Jiang
authors.forename: S C
authors.initials: SC
authors.lastname: Gautam
authors.forename: W X
authors.initials: WX
authors.lastname: Tang
authors.forename: W
authors.initials: W
authors.lastname: Tsang
authors.forename: D C
authors.initials: DC
authors.lastname: Anderson
authors.forename: A M
authors.initials: AM
authors.lastname: Manning
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002536:Cerebral Arteries / Q000502:physiology; D005353:Fibronectins / Q000378:metabolism; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014404:Tubulin / Q000096:biosynthesis
chemical_list: D005353:Fibronectins; D012333:RNA, Messenger; D014404:Tubulin; D018799:Intercellular Adhesion Molecule-1