title: The network organization and the phosphorylation of cytokeratins are concomitantly modified by forskolin in the enterocyte-like differentiated Caco-2 cell line.
abstract: Confluent Caco-2 cells, originating from a human colon carcinoma, display morphological and functional characteristics of differentiated enterocytes such as the presence of a polarized monolayer covered by an apical brush border that express several hydrolases. The adaptation of these cells to grow in the continuous presence of forskolin, a drug known to stimulate adenylyl cyclase permanently, has been previously shown to result in a decreased apical expression of hydrolases and in morphological alterations including the disappearance of intercellular spaces and shortening of microvilli. In the present work we have analyzed the possibility that cytoskeletal proteins may be the target of forskolin in living Caco-2 cells. We show that forskolin initiates dramatic changes in the spatial organization of the cytokeratin network that correlate with an increased phosphorylation of cytokeratin molecules, whereas microtubules, microfilaments and vimentin remain mainly unaffected. Indirect immunofluorescence studies show that the cytokeratin network is redistributed from the cell periphery to the cytoplasm. Biochemical experiments indicate that forskolin doesn't interfere with the cytokeratin profile, since the three cytokeratins normally found in intestine (CK 8, CK 18, CK 19) are similarly expressed in both control and forskolin-Caco-2 cells. Analysis of 32P-labeled cytokeratin extracted from the two cell populations demonstrates that forskolin quantitatively increases the phosphorylation of type I cytokeratin (CK 18 and CK 19), whereas the phosphorylation of type II cytokeratin (CK 8) is altered both quantitatively and qualitatively with the emergence of a new phosphorylation site. These results provide a new cell system in which it is possible to control the subcellular distribution of cytokeratin by changing their phosphorylation status and therefore to study their potential cellular functions.
authors.affiliation: Unité de recherches sur la neuroendocrinologie et la biologie cellulaire digestives, INSERM U. 410, CHU X. Bichat, Paris, France.
authors.forename: L
authors.initials: L
authors.lastname: Baricault
authors.forename: B
authors.initials: B
authors.lastname: de Néchaud
authors.forename: C
authors.initials: C
authors.lastname: Sapin
authors.forename: P
authors.initials: P
authors.lastname: Codogno
authors.forename: P
authors.initials: P
authors.lastname: Denoulet
authors.forename: G
authors.initials: G
authors.lastname: Trugnan
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D005576:Colforsin / Q000494:pharmacology*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D007633:Keratins / Q000378:metabolism / Q000494:pharmacology* / Q000648:ultrastructure; D010766:Phosphorylation; D014407:Tumor Cells, Cultured
chemical_list: D005576:Colforsin; D007633:Keratins