Oligoclonal beta-galactoside-specific gamma immunoglobulins allow the immunocytochemical detection of cellular antigenic determinants expressed in mouse brain after surgical injury.
Histological brain sections were probed with human oligoclonal lectin-like IgGs (L-IgG) purified from normal serum. In intact brain, antigenic determinants for these IgG were restricted to some blood vessel endothelial cells. By contrast, during the inflammatory reaction following a surgical injury, these determinants were detected at the cell surface of different cell types, within and near the lesion site. The cells reacting with L-IgG consisted of endothelial cell, mature astrocytes, activated microglial and ependymal cells.
Joubert-Caron
R
R
Laboratoire de Biochimie et Technologie des Protéines, Université Paris-Nord, UFR Biomédicale, Bobigny, France.
Lutomski
D
D
Le Saux
F
F
Quinonero
J
J
Caron
M
M
Davenne
Y
Y
Bladier
D
D
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D001253:Astrocytes / Q000201:enzymology / Q000378:metabolism; D001921:Brain / Q000201:enzymology* / Q000473:pathology; D001930:Brain Injuries / Q000201:enzymology* / Q000473:pathology; D003001:Cloning, Molecular; D000939:Epitopes / Q000276:immunology / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D007797:Laminin / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains; D001616:beta-Galactosidase / Q000378:metabolism*; D005719:gamma-Globulins
D000954:Antigens, Surface; D000939:Epitopes; D007074:Immunoglobulin G; D007797:Laminin; D005719:gamma-Globulins; D001616:beta-Galactosidase