Quantitative and qualitative analysis of exogenous gene expression by the S1 nuclease protection assay.
A method for determining the beginning and end of gene transcripts along with the position of intron/exon boundaries by S1 nuclease protection is described. Probe strategies and hybridization conditions are considered in detail. Under conditions of probe excess the method is quantitative.
Walmsley
M E
ME
Developmental Biology Research Centre, King's College, University of London, UK.
Patient
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RK
D001709:Biotechnology; D015342:DNA Probes / Q000302:isolation & purification; D005091:Exons; D015870:Gene Expression; D005821:Genetic Techniques; D007438:Introns; D015336:Molecular Probe Techniques; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D015719:Single-Strand Specific DNA and RNA Endonucleases; D014158:Transcription, Genetic
D015342:DNA Probes; D012313:RNA; D015719:Single-Strand Specific DNA and RNA Endonucleases