A new procedure for efficient recovery of DNA, RNA, and proteins from Listeria cells by rapid lysis with a recombinant bacteriophage endolysin.
A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures. Moreover, this approach should be very useful in analytical detection and differentiation of Listeria strains when the release of native nucleic acids or proteins is required.
Loessner
M J
MJ
Forschungszentrum für Milch und Lebensmittel Weihenstephan, Technische Universität München, Freising, Germany.
Schneider
A
A
Scherer
S
S
D001426:Bacterial Proteins / Q000302:isolation & purification*; D001433:Bacteriolysis / Q000502:physiology; D001435:Bacteriophages / Q000737:chemistry; D004269:DNA, Bacterial / Q000302:isolation & purification*; D010450:Endopeptidases / Q000235:genetics / Q000302:isolation & purification; D008089:Listeria monocytogenes / Q000737:chemistry*; D012329:RNA, Bacterial / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D011994:Recombinant Proteins; D010450:Endopeptidases; C001143:endolysin