Myelin basic protein does not have a mitogenic effect on adult oligodendrocytes.
Increased numbers of oligodendrocytes and remyelination are frequently observed in multiple sclerosis plaques. It is presumed the increased numbers of oligodendrocytes are due to cell division, but this has not been proven. The mitogens within the lesion which might be responsible for this are unknown. Since oligodendrocyte proliferation occurs in areas in which there is myelin breakdown, we undertook the present study to determine if myelin basic protein (MBP) or its breakdown products could induce oligodendrocyte proliferation. MBP, or MBP digested by the neutral proteinase plasmin, was added in three concentrations to the media of adult bovine oligodendrocytes in culture. Oligodendrocytes were identified by staining for galactocerebroside. Bromodeoxyuridine incorporation was used as a measure of cell division. Oligodendrocytes were found to divide only rarely in regular culture media, in the presence of MBP, plasmin, or MBP digested by plasmin. The results indicate that MBP is not a significant mitogen for the mature oligodendrocyte.
Moore
G R
GR
Department of Pathology and Laboratory Medicine (Neuropathology), Vancouver Hospital and Health Sciences Center, B.C., Canada.
Kim
S U
SU
Chang
E
E
Kim
M
M
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004591:Electrophoresis, Polyacrylamide Gel; D005341:Fibrinolysin / Q000378:metabolism; D005699:Galactosylceramides / Q000378:metabolism; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D008934:Mitogens / Q000494:pharmacology*; D004676:Myelin Basic Protein / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D009836:Oligodendroglia / Q000187:drug effects* / Q000648:ultrastructure
D005699:Galactosylceramides; D008934:Mitogens; D004676:Myelin Basic Protein; D005341:Fibrinolysin; D001973:Bromodeoxyuridine