title: Kalicludines and kaliseptine. Two different classes of sea anemone toxins for voltage sensitive K+ channels.
abstract: New peptides have been isolated from the sea anemone Anemonia sulcata which inhibit competitively the binding of 125I-dendrotoxin I (a classical ligand for K+ channel) to rat brain membranes and behave as blockers of voltage-sensitive K+ channels. Sea anemone kalicludines are 58-59-amino acid peptides cross-linked with three disulfide bridges. They are structurally homologous both to dendrotoxins which are snake venom toxins and to the basic pancreatic trypsin inhibitor (Kunitz inhibitor) and have the unique property of expressing both the function of dendrotoxins in blocking voltage-sensitive K+ channels and the function of the Kunitz inhibitor in inhibiting trypsin. Kaliseptine is another structural class of peptide comprising 36 amino acids with no sequence homology with kalicludines or with dendrotoxins. In spite of this structural difference, it binds to the same receptor site as dendrotoxin and kalicludines and is as efficient as a K+ channel inhibitor as the most potent kalicludine.
authors.affiliation: Institut de Pharmacologie Moléculaire et Cellulaire, Valbonne, France.
authors.forename: H
authors.initials: H
authors.lastname: Schweitz
authors.forename: T
authors.initials: T
authors.lastname: Bruhn
authors.forename: E
authors.initials: E
authors.lastname: Guillemare
authors.forename: D
authors.initials: D
authors.lastname: Moinier
authors.forename: J M
authors.initials: JM
authors.lastname: Lancelin
authors.forename: L
authors.initials: L
authors.lastname: Béress
authors.forename: M
authors.initials: M
authors.lastname: Lazdunski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D007611:Aprotinin / Q000737:chemistry; D003064:Cnidarian Venoms / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004546:Elapid Venoms / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D026902:Potassium Channel Blockers; D051381:Rats; D015222:Sodium Channels / Q000187:drug effects; D014981:Xenopus
chemical_list: D003064:Cnidarian Venoms; D004546:Elapid Venoms; D026902:Potassium Channel Blockers; D015222:Sodium Channels; C096147:kalicludine; C096146:kaliseptine; C020273:dendrotoxin; D007611:Aprotinin