Sink limitation induces the expression of multiple soybean vegetative lipoxygenase mRNAs while the endogenous jasmonic acid level remains low.
The response of individual members of the lipoxygenase multigene family in soybeans to sink deprivation was analyzed. RNase protection assays indicated that a novel vegetative lipoxygenase gene, vlxC, and three other vegetative lipoxygenase mRNAs accumulated in mature leaves in response to a variety of sink limitations. These data suggest that several members of the lipoxygenase multigene family are involved in assimilate partitioning. The possible involvement of jasmonic acid as a signaling molecule regulating assimilate partitioning into the vegetative storage proteins and lipoxygenases was directly assessed by determining the endogenous level of jasmonic acid in leaves from plants with their pods removed. There was no rise in the level of endogenous jasmonic acid coincident with the strong increase in both vlxC and vegetative storage protein VspB transcripts in response to sink limitation. Thus, expression of the vegetative lipoxygenases and vegetative storage proteins is not regulated by jasmonic acid in sink-limited leaves.
Bunker
T W
TW
Department of Botany, Washington State University, Pullman 99164-4238, USA.
Koetje
D S
DS
Stephenson
L C
LC
Creelman
R A
RA
Mullet
J E
JE
Grimes
H D
HD
D000595:Amino Acid Sequence; D001483:Base Sequence; D003517:Cyclopentanes / Q000378:metabolism*; D018076:DNA, Complementary / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant / Q000235:genetics; D008084:Lipoxygenase / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D054883:Oxylipins; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D012641:Selection, Genetic; D015398:Signal Transduction; D013025:Glycine max / Q000235:genetics / Q000502:physiology*
D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D010940:Plant Proteins; D012333:RNA, Messenger; C078149:vspB protein, Glycine max; C011006:jasmonic acid; D008084:Lipoxygenase