title: The expression of tumor-rejection antigen "MAGE" genes in human gastric carcinoma.
abstract: OBJECTIVE
  The genes MAGE-1 and MAGE-3 both encode melanoma peptide antigens recognized by major histocompatibility complex-restricted cytotoxic T lymphocytes. The antigens may be a target for immunotherapy. There is, however, little information on the expression of these genes in gastric carcinomas. Therefore, the expression of MAGE genes in gastric carcinomas was evaluated.
  
  
  METHODS
  The expression of MAGE-1, MAGE-2, and MAGE-3 genes in tumors and corresponding normal tissue specimens was studied using a reverse-transcription polymerase chain reaction. The results were analyzed according to clinicopathologic factors of the tumor.
  
  
  RESULTS
  In the 68 gastric carcinomas studied, MAGE-1, MAGE-2, and MAGE-3 messenger RNA were detected in 41%, 31%, and 38%, respectively. Fifty percent of the gastric carcinomas expressed at least one of the MAGE genes. Messenger RNA for the three MAGE proteins was not detected in normal gastric tissue. MAGE gene expression in gastric carcinomas was not associated with a significant clincopathology of the tumor. However, gene expression was lower in mucinous carcinomas (3 of 10).
  
  
  CONCLUSIONS
  MAGE-1, MAGE-2, and MAGE-3 are expressed in a high percentage of gastric carcinomas. These tumor rejection antigens may provide tumor-specific targets for immunotherapy.
authors.affiliation: Department of Surgery, Kyushu University, Beppu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Mori
authors.forename: M
authors.initials: M
authors.lastname: Honda
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: K
authors.initials: K
authors.lastname: Shibuta
authors.forename: K
authors.initials: K
authors.lastname: Mimori
authors.forename: H
authors.initials: H
authors.lastname: Ueo
authors.forename: T
authors.initials: T
authors.lastname: Akiyoshi
mesh_terms: D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000235:genetics*; D001483:Base Sequence; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D058950:Melanoma-Specific Antigens; D008875:Middle Aged; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins; D012333:RNA, Messenger / Q000032:analysis; D013270:Stomach / Q000737:chemistry; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D000951:Antigens, Neoplasm; C072011:MAGEA1 protein, human; C072013:MAGEA3 protein, human; C072012:MAGEB2 protein, human; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D012333:RNA, Messenger