title: The ocular surface as part of the mucosal immune system: conjunctival mucosa-specific lymphocytes in ocular surface pathology.
abstract: The mucosal immune system includes mucus membranes of the gut, respiratory and urogenital tracts. Mucosa-specific, intraepithelial lymphocytes (IELs), that correspond to the suppressor/cytotoxic subset and also express the human mucosal lymphocyte antigen (HML-1), are a unique component of this system. We have recently demonstrated these cells in the human conjunctiva, establishing the ocular surface as an integral part of the mucosal immune system. In this study we examined the distribution of lymphocyte subsets, with particular attention to mucosa-specific lymphocytes, in two ocular surface disorders, namely conjunctival intraepithelial neoplasia (CIN) and ocular cicatricial pemphigoid (OCP). Cryosections of biopsy specimen were immunostained using a panel of monoclonal antibodies against different lymphocyte subsets. In CIN, the CD8/HML ratio was decreased (1 +/- 0) and CD8/CD4 ratio was reversed (0.54 +/- 0.21). HML-1+ cells were distributed throughout the epithelial layers of dysplastic tissue. Biopsy specimens of OCP showed normal ratios of CD8/HML (1.4 +/- 0.16) but the CD8/CD4 was low (1.29 +/- 0.88). Association of HML-1+ cells with the basal layer of normal epithelium and with all layers of dysplastic epithelium suggests that expression of HML-1 antigen may be induced by actively dividing cells. HML-1+ cells may have a role in immune mechanisms associated with ocular surface disorders.
authors.affiliation: University Hospital, Queen's Medical Centre, University of Nottingham, UK.
authors.forename: H S
authors.initials: HS
authors.lastname: Dua
authors.forename: J A
authors.initials: JA
authors.lastname: Gomes
authors.forename: L A
authors.initials: LA
authors.lastname: Donoso
authors.forename: P R
authors.initials: PR
authors.lastname: Laibson
mesh_terms: D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis; D003228:Conjunctiva / Q000276:immunology*; D003229:Conjunctival Diseases / Q000473:pathology*; D003230:Conjunctival Neoplasms / Q000473:pathology*; D016207:Cytokines / Q000032:analysis; D005260:Female; D006801:Humans; D007107:Immune System / Q000166:cytology; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000276:immunology; D010390:Pemphigoid, Benign Mucous Membrane / Q000473:pathology*
chemical_list: D000951:Antigens, Neoplasm; D016207:Cytokines