title: Salmonella strain secreting active listeriolysin changes its intracellular localization.
abstract: We describe the construction of an attenuated Salmonella dublin aroA strain which secretes via the Escherichia coli hemolysin secretion machinery an active hybrid cytolysin consisting of listeriolysin from Listeria monocytogenes and the C-terminal secretion signal of E. coli hemolysin. This hemolytic S. dublin strain is partially released into the cytoplasm of the host cell following uptake by J774 macrophage cells, whereas the nonhemolytic control S. dublin aroA strain remains in the phagosome.
authors.affiliation: Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften, Würzburg, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Gentschev
authors.forename: Z
authors.initials: Z
authors.lastname: Sokolovic
authors.forename: H J
authors.initials: HJ
authors.lastname: Mollenkopf
authors.forename: J
authors.initials: J
authors.lastname: Hess
authors.forename: S H
authors.initials: SH
authors.lastname: Kaufmann
authors.forename: M
authors.initials: M
authors.lastname: Kuhn
authors.forename: G F
authors.initials: GF
authors.lastname: Krohne
authors.forename: W
authors.initials: W
authors.lastname: Goebel
mesh_terms: D000818:Animals; D001427:Bacterial Toxins; D001483:Base Sequence; D002460:Cell Line; D006360:Heat-Shock Proteins / Q000502:physiology*; D006460:Hemolysin Proteins / Q000502:physiology*; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008969:Molecular Sequence Data; D012475:Salmonella / Q000502:physiology*
chemical_list: D001427:Bacterial Toxins; D006360:Heat-Shock Proteins; D006460:Hemolysin Proteins; C038705:hlyA protein, Listeria monocytogenes