Strain typing of Lentinula edodes by random amplified polymorphic DNA assay.
Single 10-base primers were used to generate randomly amplified polymorphic DNA (RAPD) markers in the shittake mushroom, Lentinula edodes. Seven primers produced polymorphisms in all 15 strains tested, producing 12-19 bands ranging from 0.34 to 2.52 kb. Thirteen of the 15 strains had unique DNA fingerprints, whereas L. edodes ATCC 28759 and ATCC 28760 exhibited identical RAPD profiles for all the primers. Molecular-genetic markers obtained with the RAPD assay can be used to differentiate strains of L. edodes and have potential applicatiosn in mushroom breeding and strain improvement programs.
Zhang
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Mycology and Botany Department, American Type Culture Collection, Rockville, MD 20852-1776, USA.
Molina
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D001483:Base Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics*; D005784:Gene Amplification; D005819:Genetic Markers; D005820:Genetic Testing; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000235:genetics*; D011124:Polyporaceae / Q000145:classification / Q000235:genetics*; D011897:Random Allocation
D017931:DNA Primers; D004271:DNA, Fungal; D005819:Genetic Markers