title: Sequence of an ammonia monooxygenase subunit A-encoding gene from Nitrosospira sp. NpAV.
abstract: One of three gene copies encoding ammonia monooxygenase subunit A (AmoA) and flanking sequences was isolated from genomic DNA of the chemolithotrophic soil bacterium Nitrosospira sp. NpAV using oligodeoxyribonucleotide primers and the polymerase chain reaction (PCR). The gene (amoA) encodes a 274-amino-acid polypeptide which has similarity to the ammonia monooxygenase acetylene-binding protein from Nitrosomonas europaea.
authors.affiliation: USU Program in Molecular Biology, Logan, UT 84322, USA.
authors.forename: M G
authors.initials: MG
authors.lastname: Klotz
authors.forename: J M
authors.initials: JM
authors.lastname: Norton
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000737:chemistry / Q000378:metabolism; D005798:Genes, Bacterial; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009606:Nitrosomonas / Q000235:genetics; D010088:Oxidoreductases / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012988:Soil Microbiology
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D046911:Macromolecular Substances; D010088:Oxidoreductases; C045582:ammonia monooxygenase