High frequency of mutagen-induced chromatid exchanges at interstitial telomere-like DNA sequence blocks of Chinese hamster cells.
Interstitial telomere-like DNA sequence arrays of Chinese hamster Don cells were delineated by fluorescence in situ hybridization in quadriradial and triradial chromosome configurations induced by X-rays, mitomycin C and teniposide (VM-26). Around 40% of the scored exchanges involved a telomeric-like block of sequences at a rearrangement site. This frequency was independent of the DNA-damaging agent, and the result suggests a general recombinogenic capacity of interstitial telomere-like DNA sequence repeats that, at least in the case of the agents employed, seems not to be related to the initial mechanism of DNA damage.
Fernández
J L
JL
Centro Oncológico de Galicia, La Coruña, Spain.
Gosálvez
J
J
Goyanes
V
V
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004247:DNA / Q000187:drug effects* / Q000235:genetics; D004249:DNA Damage; D017404:In Situ Hybridization, Fluorescence; D009153:Mutagens / Q000633:toxicity*; D012854:Sister Chromatid Exchange; D016615:Telomere
D009153:Mutagens; D004247:DNA