Protein A in Staphylococcus aureus isolates from pigs.
The presence and quantity of protein A in Staphylococcus aureus 147 isolates from the tonsils of healthy pigs were examined by three methods. Cell-bound protein A was detected in 71 (48%), 104 (71%) and 123 (84%) of 147 isolates by the slide hemagglutination test, microplate hemagglutination test and enzyme-linked immunosorbent assay (ELISA), respectively. Extracellular protein A was not detected in any isolates by the microplate hemagglutination test. When the quantity of cell-bound protein A in the isolates was determined by the ELISA, most of the isolates contained about 0.8 to 2.2 micrograms of protein A/ml in bacterial cell suspensions of a concentration of MacFarland No. 3.
Takeuchi
S
S
Department of Bioscience, Faculty of Biotechnology, Fukui Prefectural University, Japan.
Matuda
K
K
Sasano
K
K
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D004912:Erythrocytes; D006386:Hemagglutination Tests; D012756:Sheep; D013205:Staphylococcal Protein A / Q000032:analysis*; D013211:Staphylococcus aureus / Q000145:classification / Q000302:isolation & purification*; D013552:Swine / Q000382:microbiology*
D013205:Staphylococcal Protein A