Lipofection reagents prepared by a simple ethanol injection technique.
Cationic liposomes are being utilized for the delivery of DNA into mammalian cells both in vitro and in vivo. In this report, we describe a rapid, simple injection method for the preparation of cationic liposomes that requires no special equipment. We demonstrate that, using this injection method, liposomes prepared with a commercially available cationic lipid (dimethyldioctadecylammonium bromide, DDAB) or a synthetic cationic cholesterol derivative, along with a neutral lipid (dioleoyl-L-alpha-phosphatidylethanolamine, DOPE), effectively transfect a variety of cell lines in vitro. The transfection activity of liposomes prepared by this method was comparable to that obtained with liposomes prepared by the standard evaporation/sonication procedure and to that of a commercial reagent, Lipofectamine.
Campbell
M J
MJ
Department of Surgery, Stanford University School of Medicine, CA 94305, USA.
D002412:Cations; D002460:Cell Line / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D000431:Ethanol; D007202:Indicators and Reagents / Q000494:pharmacology*; D008081:Liposomes; D008845:Microinjections / Q000379:methods*; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; D014162:Transfection / Q000379:methods*
D002412:Cations; D007202:Indicators and Reagents; D008081:Liposomes; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; C046112:didodecyldimethylammonium; D000431:Ethanol; C020888:1,2-dielaidoylphosphatidylethanolamine; D004247:DNA