Photodynamic therapy of experimental choroidal neovascularization using lipoprotein-delivered benzoporphyrin.
OBJECTIVE
To investigate photodynamic therapy of experimental choroidal neovascularization using benzoporphyrin derivative monoacid (Verteporfin).


METHODS
Photodynamic therapy using benzoporphyrin derivative monoacid was investigated in cynomolgus monkeys. Following intravenous injection of benzoporphyrin derivative monoacid (1 to 2 mg/kg) complexed with low-density lipoprotein, the eyes were irradiated with 692-nm light at a fluence of 50 to 150 J/cm2 and irradiance of 150 to 600 mW/cm2. Choroidal neovascularization was documented before photodynamic therapy and closure was demonstrated by fundus photography, fluorescein angiography, and light and electron microscopic examination.


RESULTS
Following photodynamic therapy, vessels within choroidal neovascularization were occluded, and there was damage to the choroidal neovascularization endothelium and the subjacent choriocapillaris. Damage to the retinal pigment epithelium and photoreceptors was also observed.


CONCLUSIONS
Photodynamic therapy with lipoprotein-delivered benzoporphyrin derivative monoacid was effective in this animal model of choroidal neovascularization and may be a promising, potentially selective, therapy for choroidal neovascularization.
Miller
J W
JW
Laser Research Laboratory, Massachusetts Eye and Ear Infirmary, USA.
Walsh
A W
AW
Kramer
M
M
Hasan
T
T
Michaud
N
N
Flotte
T J
TJ
Haimovici
R
R
Gragoudas
E S
ES
D000818:Animals; D002829:Choroid / Q000098:blood supply* / Q000648:ultrastructure; D004195:Disease Models, Animal; D016503:Drug Delivery Systems; D005451:Fluorescein Angiography; D005654:Fundus Oculi; D007275:Injections, Intravenous; D007834:Lasers; D008074:Lipoproteins; D008252:Macaca fascicularis; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000473:pathology; D010778:Photochemotherapy; D011166:Porphyrins / Q000008:administration & dosage / Q000627:therapeutic use*; D000077362:Verteporfin
D008074:Lipoproteins; D011166:Porphyrins; D000077362:Verteporfin