title: Expression of human chorionic gonadotropin beta subunit genes in superficial and invasive bladder carcinomas.
abstract: Increased serum levels of human chorionic gonadotropin beta subunit (hCG beta) were described previously in patients with bladder cancer. To obtain insight into such production of hCG beta, the expression of hCG beta 7, 8, 5, and 3 genes in bladder carcinomas and normal urothelia was investigated by reverse transcription PCR. Surprisingly, hCG beta mRNAs were detected in both normal urothelial and carcinomatous cells. However, tumor progression was characterized by different patterns of transcription of the hCG beta genes; the beta 7 gene was the only gene transcribed in normal urothelia and Ta tumors included in this study, whereas in addition to beta 7, genes beta 5, 8, and 3 were transcribed in T1 to T4 tumors. Moreover, transcription levels of the latter three genes increased with the stage of the disease. These observations showed that dramatic modifications in the expression of hCG beta genes accompany progression of bladder carcinomas.
authors.affiliation: Laboratoire d'Immunologie des Tumeurs, URA 1484 CNRS, Faculté des Sciences Pharmaceutiques et Biologiques de Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: Lazar
authors.forename: S G
authors.initials: SG
authors.lastname: Diez
authors.forename: A
authors.initials: A
authors.lastname: Laurent
authors.forename: Y
authors.initials: Y
authors.lastname: Giovangrandi
authors.forename: F
authors.initials: F
authors.lastname: Radvanyi
authors.forename: D
authors.initials: D
authors.lastname: Chopin
authors.forename: J M
authors.initials: JM
authors.lastname: Bidart
authors.forename: D
authors.initials: D
authors.lastname: Bellet
authors.forename: M
authors.initials: M
authors.lastname: Vidaud
mesh_terms: D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D006063:Chorionic Gonadotropin / Q000032:analysis* / Q000235:genetics; D018997:Chorionic Gonadotropin, beta Subunit, Human; D006801:Humans; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis*; D012334:RNA, Neoplasm / Q000032:analysis*; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
chemical_list: D014408:Biomarkers, Tumor; D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D010446:Peptide Fragments; D012333:RNA, Messenger; D012334:RNA, Neoplasm