title: Collectin-43 is a serum lectin with a distinct pattern of carbohydrate recognition.
abstract: Collectin-43 (CL-43) is a C-type serum lectin and a member of the collectin family of soluble proteins that are effector molecules in innate immunity. We have investigated the binding specificity of CL-43 using as model systems a panel of structurally defined oligosaccharides in the form of neoglycolipids, and several glycoproteins derived from the complement glycoprotein C3 during activation of the complement cascade. A specificity is revealed towards fucose as part of the Lea oligosaccharide sequence, and towards mannose as found on high mannose-type chains. These are features shared with other serum collectins, conglutinin and mannan-binding proteins; a major difference is the lack of detectable binding by CL-43 to N-glycosidic oligosaccharides terminating in N-acetylglucosamine. CL-43 has a unique pattern of reactivity towards high mannose-type oligosaccharides on the two glycosylation sites of C3 and derived glycoproteins: it binds to C3c (not bound by conglutinin and mannan-binding protein) but not to hydrolysed C3 [C3(H2O)], C3b or iC3b immobilized on microwells (all bound by conglutinin but not by mannan-binding protein). When these glycoproteins are sodium dodecyl sulphate (SDS)-treated and immobilized on nitrocellulose, CL-43 (but not conglutinin nor mannan-binding protein) binds strongly to C3(H2O), iC3b and C3c. The salient conclusions are, first, that there are remarkable positive or negative effects of carrier protein on oligosaccharide presentation and these differ for the individual collectins. Second, the different though partially overlapping binding patterns among the collectins may be important for their function as circulating effector molecules with broad surveillance capabilities.
authors.affiliation: Glycosciences Laboratory, Northwick Park Hospital, Harrow, UK.
authors.forename: R W
authors.initials: RW
authors.lastname: Loveless
authors.forename: U
authors.initials: U
authors.lastname: Holmskov
authors.forename: T
authors.initials: T
authors.lastname: Feizi
mesh_terms: D000818:Animals; D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002417:Cattle; D037222:Collectins; D003176:Complement C3 / Q000378:metabolism; D006017:Glycolipids / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000378:metabolism; D012712:Serum Globulins / Q000378:metabolism*
chemical_list: D037222:Collectins; D003176:Complement C3; D006017:Glycolipids; D006023:Glycoproteins; D037102:Lectins; D011134:Polysaccharides; D012712:Serum Globulins; C086954:collectin-43 protein, bovine