title: Human chromosome 11 suppresses the tumorigenicity of adenovirus transformed baby rat kidney cells: involvement of the Wilms' tumor 1 gene.
abstract: Human chromosome 11 was introduced into adenovirus-transformed baby rat kidney (BRK) cells by microcell-mediated chromosome transfer. The resulting microcell hybrids (MCHs) showed a reduced ability to form tumors upon s.c. injection into athymic mice. Further analysis, with the use of defined deletion chromosomes of 11p, indicated that the presence of region 11p13-p12 is necessary for the suppression of tumorigenicity. In contrast, the presence of region 11p15-14.1 appeared to increase the rate of tumor growth. Expression studies on the human Wilms' tumor I (WTI) and the insulin-like growth factor II (IGF-II) genes, which lie in regions 11p13 and 11p15, respectively, suggested the involvement of both genes in determining the degree of suppression of tumorigenicity. Finally, stable expression of a murine WTI protein in the adenovirus-transformed cells resulted in almost complete suppression of tumorigenicity, establishing the WTI protein as a tumor suppressor in this cell system.
authors.affiliation: Laboratory for Molecular Carcinogenesis, Leiden University, The Netherlands.
authors.forename: A L
authors.initials: AL
authors.lastname: Menke
authors.forename: R C
authors.initials: RC
authors.lastname: van Ham
authors.forename: E
authors.initials: E
authors.lastname: Sonneveld
authors.forename: A
authors.initials: A
authors.lastname: Shvarts
authors.forename: E J
authors.initials: EJ
authors.lastname: Stanbridge
authors.forename: K
authors.initials: K
authors.lastname: Miyagawa
authors.forename: A J
authors.initials: AJ
authors.lastname: van der Eb
authors.forename: A G
authors.initials: AG
authors.lastname: Jochemsen
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics; D000818:Animals; D002455:Cell Division; D002472:Cell Transformation, Viral; D002880:Chromosomes, Human, Pair 11; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D018014:Gene Transfer Techniques; D016162:Genes, Wilms Tumor; D011905:Genes, ras; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000235:genetics*; D051381:Rats; D009396:Wilms Tumor / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D007335:Insulin-Like Growth Factor II