Effects of galanin on short circuit current and electrolyte transport in rabbit ileum.
Galanin decreased short circuit current (Isc) and increased active Na+ and Cl- absorption in rabbit ileum. In the absence of calcium, the galanin-induced decrease in Isc was inhibited by approximately 60%. Tetrodotoxin significantly reduced the effect of galanin on Isc, and tetrodotoxin and EGTA totally blocked the effect, indicating that the nonneuronal mediator of the effect is Ca2+ dependent. Galanin binding to basolateral membranes prepared from ileal epithelial cells was specific and of high affinity. These results suggest the involvement of this peptide in the regulation of intestinal epithelial cell function.
Homaidan
F R
FR
Department of Pharmacology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
Tang
S H
SH
Donowitz
M
M
Sharp
G W
GW
D000818:Animals; D002118:Calcium / Q000494:pharmacology / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D004553:Electric Conductivity; D004848:Epithelium / Q000378:metabolism; D019004:Galanin; D007082:Ileum / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008297:Male; D009479:Neuropeptides / Q000494:pharmacology; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D011817:Rabbits; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone / Q000378:metabolism; D012964:Sodium / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
D002712:Chlorides; D009479:Neuropeptides; D010455:Peptides; D044088:Receptors, Galanin; D011964:Receptors, Gastrointestinal Hormone; D013779:Tetrodotoxin; D004533:Egtazic Acid; D019004:Galanin; D012964:Sodium; D002118:Calcium