title: Decrease of heparan sulfate staining in the glomerular basement membrane in murine lupus nephritis.
abstract: Recently we found in biopsies of human lupus nephritis a nearly complete loss of heparan sulfate (HS) staining in the glomerular basement membrane (GMB). To clarify the relationship between HS staining and albuminuria in lupus nephritis, we studied MRL/lpr mice with short (< 7 days) or prolonged duration of albuminuria (14-21 days) and compared these with mice of different ages without albuminuria. Kidney sections were stained for mouse immunoglobulin (Ig), HS, heparan sulfate proteoglycan (HSPG)-core protein and laminin in immunofluorescence. In mice with prolonged albuminuria HS staining in the glomerular capillary loops had almost completely disappeared, whereas staining was unaltered in non-albuminuric mice (P = 0.001). In mice with short duration of albuminuria, there was a tendency toward a decrease of HS staining (P = 0.06). The expression of HSPG-core protein and other extra cellular matrix (ECM) components was unaltered in all groups. HS staining correlated inversely with albuminuria (rs = -0.55; P < 0.001) and with staining of Ig deposits in the capillary loops (rs = -0.74; P < 0.001). Despite the nearly complete loss of HS staining in the GBM in mice with prolonged albuminuria, there was no change in glomerular HS content as assessed by agarose electrophoresis and HS inhibition ELISA. We conclude that the development of albuminuria in MRL/lpr mice is accompanied by a loss of HS staining in the GBM, probably due to the masking of HS by deposits of Ig. In vitro studies revealed that autoantibodies complexed to nucleosomal antigens can inhibit the binding of the anti-HS monoclonal antibody to HS. Whether this also occurs in vivo remains to be determined.
authors.affiliation: Department of Nephrology, University Hospital Nijmegen, The Netherlands.
authors.forename: M C
authors.initials: MC
authors.lastname: van Bruggen
authors.forename: K
authors.initials: K
authors.lastname: Kramers
authors.forename: M N
authors.initials: MN
authors.lastname: Hylkema
authors.forename: J
authors.initials: J
authors.lastname: van den Born
authors.forename: M A
authors.initials: MA
authors.lastname: Bakker
authors.forename: K J
authors.initials: KJ
authors.lastname: Assmann
authors.forename: R J
authors.initials: RJ
authors.lastname: Smeenk
authors.forename: J H
authors.initials: JH
authors.lastname: Berden
mesh_terms: D000419:Albuminuria / Q000652:urine; D000818:Animals; D001485:Basement Membrane / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006497:Heparan Sulfate / Q000032:analysis*; D007668:Kidney / Q000473:pathology; D007678:Kidney Glomerulus / Q000737:chemistry*; D008181:Lupus Nephritis / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008815:Mice, Inbred Strains; D013194:Staining and Labeling
chemical_list: D006497:Heparan Sulfate