HIV infection of choriocarcinoma cell lines derived from human placenta: the role of membrane CD4 and Fc-Rs into HIV entry.
We have shown previously that trophoblast cells from human placenta can be infected with HIV-1 and a productive infection established. Recently, (1991, J. Virol. 65, 2102-2107) Zachar et al. and D. M. Phillips and X. Tan (1992, AIDS Res. Hum. Retroviruses 8, 1697-1705) have described in vitro infection of choriocarcinoma cell lines. Using choriocarcinoma cell lines (JAR, BeWo, and FD25, a trophoblast-derived cell line) we have infected these cells with several laboratory strains of virus and have shown that this can be prevented either by sCD4 or by antibodies to CD4. This provides strong evidence that the infection may be through CD4. In addition, we have found that infection of JAR and FD25 cells by HIV-1/Lai was enhanced in the presence of human antisera to HIV-1. This supports an additional role for immunoglobulin receptors (Fc-R) in the entry of virus into the cell. We report here evidence that CD4 and Fc-R on the cell surface play crucial roles in the entry of HIV into such placenta-derived cell lines.
David
F J
FJ
Biologie Cellulaire et Moléculaire de la Relation Materno-fetale (INSERM CJF 92 09), Hopital Antoine Béclère, Clamart, France.
Tran
H C
HC
Serpente
N
N
Autran
B
B
Vaquero
C
C
Djian
V
V
Menu
E
E
Barré-Sinoussi
F
F
Chaouat
G
G
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens / Q000502:physiology*; D002822:Choriocarcinoma / Q000821:virology*; D005260:Female; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology / Q000502:physiology*; D015498:HIV-2 / Q000201:enzymology / Q000502:physiology*; D006801:Humans; D007106:Immune Sera; D008213:Lymphocyte Activation; D008214:Lymphocytes; D011247:Pregnancy; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011961:Receptors, Fc / Q000502:physiology*; D011994:Recombinant Proteins; D014327:Trophoblasts / Q000821:virology; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000821:virology*
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016655:HIV Core Protein p24; D007106:Immune Sera; C079851:OKT4A monoclonal antibody; D011961:Receptors, Fc; D011994:Recombinant Proteins; C076613:recombinant soluble CD4; C072416:reverse transcriptase, Human immunodeficiency virus 2; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase