Characterization of a LINE-1 cDNA that originated from RNA present in ribonucleoprotein particles: implications for the structure of an active mouse LINE-1.
Full-length, sense-strand, long interspersed element-1 (LINE-1 or L1) RNA is found as an RNA-protein complex in mouse embryonal carcinoma cells. Since this complex is a likely intermediate in LINE-1 transposition, its RNA may be enriched for the functional, or active, subset of mouse L1 sequences. For this reason, a cDNA library was constructed from RNA prepared from these ribonucleoprotein particles. The isolation and complete DNA sequence of one clone that is a strong candidate to be a functional version of mouse L1 is reported here. The structure of this element suggests a revision of the predicted sequence of an active mouse L1 and provides a tag that can be used to isolate its locus in the genome.
Martin
S L
SL
Department of Cellular and Structural Biology, University of Colorado School of Medicine, Denver 80262.
D000818:Animals; D001483:Base Sequence; D018236:Carcinoma, Embryonal; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D012313:RNA / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D018626:Retroelements / Q000235:genetics*; D012261:Ribonucleoproteins / Q000737:chemistry; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D018626:Retroelements; D012261:Ribonucleoproteins; D012313:RNA