Expression of cell surface markers during differentiation of CD34+, CD38-/lo fetal and adult bone marrow cells.
Even though there has been considerable progress in the phenotypic characterization of CD34+ bone marrow cells, there is still limited knowledge about the cell phenotypes corresponding to functional terms such as colony-forming cells, burst-forming cells, long-term culture-initiating cells, and high-proliferative potential cells. In this study, we performed a detailed analysis of phenotypic characteristics of subsets of CD34+ cells. We compared cells from adult and fetal bone marrow to investigate whether reported functional differences are reflected in the cellular phenotypes. CD34+, CD38-/lo, HLA-DR+ cells, which have been shown to contain the most immature hematopoietic progenitor cells, stained as a homogeneous population with most monoclonal antibodies (mAbs). The antigens sLex, CD32, and CD7 were, however, heterogeneously expressed in the CD38-/lo population. Phenotypic differences in the CD34+, CD38-/lo population was found when comparing adult and fetal bone marrow cells. Adult bone marrow CD34+, CD38-/lo cells stained more brightly with CD4, Thy-1, and CD49b and more dimly with CD32 than the same population in fetal bone marrow. Certain antigens that have previously been regarded as lineage-specific were found on the CD34+, CD38-/lo, HLA-DR+ cells in both fetal and adult bone marrow. These included CD52, CD13, and CD33. The markers that were found to be most useful in discriminating between subsets of lineage-committed cells within the CD34+, CD38+ population included the B cell marker CD19 and the granulomonocytic marker CD64. The phenotypic analysis presented here should provide a basis for establishing a better link between functional and phenotypic characteristics of hematopoietic progenitor cells.
Olweus
J
J
Becton-Dickinson Immunocytometry Systems, San Jose, California 95131, USA.
Lund-Johansen
F
F
Terstappen
L W
LW
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000328:Adult; D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology; D018952:Antigens, CD34; D000943:Antigens, Differentiation / Q000032:analysis* / Q000276:immunology; D000954:Antigens, Surface / Q000032:analysis*; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000276:immunology; D005314:Embryonic and Fetal Development / Q000502:physiology; D005434:Flow Cytometry; D006684:HLA-DR Antigens / Q000032:analysis / Q000276:immunology; D006801:Humans; D008562:Membrane Glycoproteins; D009699:N-Glycosyl Hydrolases / Q000032:analysis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018952:Antigens, CD34; D000943:Antigens, Differentiation; D000954:Antigens, Surface; D006684:HLA-DR Antigens; D008562:Membrane Glycoproteins; D009699:N-Glycosyl Hydrolases; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1