title: Crystallisation and preliminary X-ray analysis of the receptor-binding domain of human and bovine alpha 2-macroglobulin.
abstract: The receptor-binding domains (RBDs) of human and bovine alpha 2-macroglobulin (alpha 2M) have been isolated after limited proteolysis of methylamine-treated alpha 2M with papain. Single crystals of the RBDs have been grown by vapour diffusion. Crystals of human RBD are very thin plates unsuited for data collection. However, crystals of RBD from bovine alpha 2M give diffraction patterns suitable for X-ray analysis, and a complete dataset with a maximum resolution of 2.3 A has been collected with synchrotron radiation at cryogenic temperature. The crystals belong to spacegroup P3(1)21 or P3(2)21 with cell parameters a = b = 106.8 A, c = 72.2 A.
authors.affiliation: Department of Molecular Biology, University of Arhus, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Dolmer
authors.forename: L B
authors.initials: LB
authors.lastname: Jenner
authors.forename: L
authors.initials: L
authors.lastname: Jacobsen
authors.forename: G R
authors.initials: GR
authors.lastname: Andersen
authors.forename: T J
authors.initials: TJ
authors.lastname: Koch
authors.forename: S
authors.initials: S
authors.lastname: Thirup
authors.forename: L
authors.initials: L
authors.lastname: Sottrup-Jensen
authors.forename: J
authors.initials: J
authors.lastname: Nyborg
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D003460:Crystallization; D018360:Crystallography, X-Ray; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification; D011971:Receptors, Immunologic / Q000378:metabolism; D011973:Receptors, LDL / Q000378:metabolism; D016415:Sequence Alignment; D000511:alpha-Macroglobulins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D026503:Low Density Lipoprotein Receptor-Related Protein-1; D010446:Peptide Fragments; D011971:Receptors, Immunologic; D011973:Receptors, LDL; D000511:alpha-Macroglobulins