title: Comparative modeling of the three-dimensional structure of type II antifreeze protein.
abstract: Type II antifreeze proteins (AFP), which inhibit the growth of seed ice crystals in the blood of certain fishes (sea raven, herring, and smelt), are the largest known fish AFPs and the only class for which detailed structural information is not yet available. However, a sequence homology has been recognized between these proteins and the carbohydrate recognition domain of C-type lectins. The structure of this domain from rat mannose-binding protein (MBP-A) has been solved by X-ray crystallography (Weis WI, Drickamer K, Hendrickson WA, 1992, Nature 360:127-134) and provided the coordinates for constructing the three-dimensional model of the 129-amino acid Type II AFP from sea raven, to which it shows 19% sequence identity. Multiple sequence alignments between Type II AFPs, pancreatic stone protein, MBP-A, and as many as 50 carbohydrate-recognition domain sequences from various lectins were performed to determine reliably aligned sequence regions. Successive molecular dynamics and energy minimization calculations were used to relax bond lengths and angles and to identify flexible regions. The derived structure contains two alpha-helices, two beta-sheets, and a high proportion of amino acids in loops and turns. The model is in good agreement with preliminary NMR spectroscopic analyses. It explains the observed differences in calcium binding between sea raven Type II AFP and MBP-A. Furthermore, the model proposes the formation of five disulfide bridges between Cys 7 and Cys 18, Cys 35 and Cys 125, Cys 69 and Cys 100, Cys 89 and Cys 111, and Cys 101 and Cys 117.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Protein Engineering Network of Centres of Excellence, Edmonton, Canada.
authors.forename: F D
authors.initials: FD
authors.lastname: Sönnichsen
authors.forename: B D
authors.initials: BD
authors.lastname: Sykes
authors.forename: P L
authors.initials: PL
authors.lastname: Davies
mesh_terms: D000222:Adaptation, Physiological; D000595:Amino Acid Sequence; D000818:Animals; D021301:Antifreeze Proteins; D002135:Calcium-Binding Proteins / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry; D015815:Cell Adhesion Molecules / Q000737:chemistry; D003198:Computer Simulation; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D004220:Disulfides / Q000737:chemistry; D019040:E-Selectin; D005399:Fishes; D005615:Freezing; D006023:Glycoproteins / Q000737:chemistry*; D051847:Lithostathine; D009682:Magnetic Resonance Spectroscopy; D037601:Mannose-Binding Lectin; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship
chemical_list: D021301:Antifreeze Proteins; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D004220:Disulfides; D019040:E-Selectin; D006023:Glycoproteins; D051847:Lithostathine; D037601:Mannose-Binding Lectin; D009419:Nerve Tissue Proteins; C069858:mannose binding protein A