Chondroitin sulfate proteoglycan staining in astrocyte-Schwann cell co-cultures.
Transplantation of Schwann cells (SCs) in the central nervous system (CNS) for remyelination in pathological situations has been considered a promising approach. However, numerous studies have indicated that astrocytes have a restrictive effect on SC migration within the CNS. We have previously established an in vitro model which demonstrates the restrictive effect of astrocytes on SCs (Ghirnikar and Eng, Glia 4:367-377, 1994). Using this culture model, in the present study, we have characterized the molecular basis underlying astrocyte-SC interaction and demonstrated chondroitin sulfate proteoglycan (CSP) staining in the co-cultures. Following 1-2 weeks of incubation, CSP staining was specifically associated with SCs co-cultured with astrocytes. Staining with antibodies specific for the different chondroitin sulfate isomers revealed the presence of both, chondroitin-4- and 6-sulfates in SCs. In contrast, SCs when cultured alone, or in the presence of astrocytes conditioned medium did not show CSP staining. These data suggest that CSP staining is associated with SCs following co-culture with astrocytes and mediated by cell to cell contact. We hypothesize that the CSP, alone or in combination with other molecules expressed by astrocytes and/or SCs, may be involved in the restrictive effects of astrocytes on SCs. Identification of molecules involved in the unfavorable interaction between astrocytes and SCs will have an important bearing on efforts to remyelinate demyelinated axons by SC transplantation within the damaged CNS.
Ghirnikar
R S
RS
Department of Pathology, Stanford University, School of Medicine, California, USA.
Eng
L F
LF
D000818:Animals; D001253:Astrocytes / Q000737:chemistry*; D002478:Cells, Cultured / Q000737:chemistry; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis*; D007150:Immunohistochemistry; D051381:Rats; D012583:Schwann Cells / Q000737:chemistry*; D013194:Staining and Labeling
D011508:Chondroitin Sulfate Proteoglycans