Biotinylated hyaluronan as a probe for detection of binding proteins in cells and tissues.
A convenient and reliable method for preparing and using biotinylated hyaluronan for detection of hyaluronan-binding proteins is described. The biotinylated hyaluronan can be used to detect binding proteins in transblots after electrophoresis or as a histological probe for localization of binding proteins in cultured cells and tissue sections.
Yu
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Tufts University School of Medicine, Boston, MA, USA.
Toole
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BP
D000818:Animals; D001710:Biotin / Q000378:metabolism*; D002352:Carrier Proteins / Q000032:analysis*; D002356:Cartilage / Q000737:chemistry / Q000196:embryology; D002478:Cells, Cultured; D002642:Chick Embryo; D018960:Hyaluronan Receptors; D006820:Hyaluronic Acid / Q000378:metabolism*; D051379:Mice; D011956:Receptors, Cell Surface / Q000032:analysis*; D016201:Receptors, Lymphocyte Homing / Q000032:analysis*
D002352:Carrier Proteins; D018960:Hyaluronan Receptors; D011956:Receptors, Cell Surface; D016201:Receptors, Lymphocyte Homing; D001710:Biotin; D006820:Hyaluronic Acid