Induction of cytochrome P-450 by 5-methoxypsoralen in the yeast Saccharomyces cerevisiae.
Yeast cells (D7 strain) incubated in the presence of 5-methoxypsoralen (5-MOP) increase the activity of the monooxygenase system cytochrome P-450 dependent (cytochrome P-450 level and 7-ethoxycoumarin-O-diethylase activity). Northern analysis of cytochrome P-450 specific RNA shows that 5-MOP treatments induce an increase in mRNA. The induction of cytochrome P-450 appears to occur at the transcriptional level. The capacity of 5-MOP to induce the cytochrome P-450 system in eukaryotic cells, in which it is known to be involved in the metabolism of the psoralen, may decrease the availability of the compound for photo-induced genotoxic reactions, which may explain the good tolerance in patients.
Del Carratore
R
R
Istituto di Mutagenesi e Differenziamento, CNR, Pisa, Italy.
Morichetti
E
E
Bronzetti
G
G
Galeotti
C
C
D000078223:5-Methoxypsoralen; D015660:7-Alkoxycoumarin O-Dealkylase / Q000378:metabolism; D015152:Blotting, Northern; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000235:genetics; D004790:Enzyme Induction; D008730:Methoxsalen / Q000031:analogs & derivatives* / Q000494:pharmacology; D012313:RNA / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology
D000078223:5-Methoxypsoralen; D012313:RNA; D003577:Cytochrome P-450 Enzyme System; D015660:7-Alkoxycoumarin O-Dealkylase; D008730:Methoxsalen