Role of beta 1 integrins in cell spreading and migration of human nevomelanocytes and dysplastic nevi cells on collagen type IV and laminin.
We characterized beta 1 integrin subunit expression on three different cultures of benign human nevomelanocytes (NMC) and on four different cell cultures of human dysplastic nevus (DN) cells by flow cytometry analysis and examined their role in mediating cell spreading and migration on collagen type IV (CN IV) and laminin (LN) coated substrates by using a quantitative video image analysis system. The seven human NMC and DNC cultures expressed heterogeneous levels of beta 1, alpha 2, alpha 3 and alpha 6 integrin subunits. Image analysis showed that a significant increase (P < 0.001) in cell spreading and migration of the DN cells was induced on increasing coating concentrations of CN IV and LN. However, the NMC did not show an increase in cell spreading or migration on these substrates when compared to the substrates coated with denatured BSA only. The CN IV-induced cell spreading of the DN cells was significantly inhibited by anti-beta 1 mAb (AIIB2), anti-alpha 2 mAb (P1E6), or anti-alpha 3 mAb (P1B5), but not by mAb against alpha 6 integrin subunit (GoH3). The DN cell spreading on LN was not significantly inhibited by these mAbs. In contrast, the migration of the DN on CN IV and LN was significantly inhibited by anti-beta 1 mAb, anti-alpha 2 mAb, anti-alpha 3 mAb and anti-alpha 6 mAb. These data suggest that the alpha 2 and alpha 3 subunit are important for cell spreading of the DN on CN IV, although they are less important in cell spreading on the extracellular matrix component LN.(ABSTRACT TRUNCATED AT 250 WORDS)
Vink
J
J
Department of Dermatology and Pathology, University of Leiden, The Netherlands.
Dekker
S K
SK
Van Leeuwen
R L
RL
Vermeer
B J
BJ
Bruijn
J A
JA
Byers
H R
HR
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D003094:Collagen; D003470:Culture Media; D004416:Dysplastic Nevus Syndrome / Q000473:pathology*; D006801:Humans; D019012:Integrin beta1; D016023:Integrins / Q000276:immunology / Q000502:physiology*; D007797:Laminin; D008544:Melanocytes / Q000502:physiology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D003470:Culture Media; D019012:Integrin beta1; D016023:Integrins; D007797:Laminin; D003094:Collagen