Rapid detection and biovar differentiation of Ureaplasma urealyticum in clinical specimens by PCR.
On the basis of the nucleotide sequence of the multiple-banded (MB) antigen genes of Ureaplasma urealyticum, a polymerase chain reaction (PCR) technique was developed for rapid detection and biovar differentiation of U. urealyticum in a total of 100 urogenital specimens from 50 female patients. Positive PCR UM-1 amplification was found in 28 cervical swabs and 31 urine samples. Overall agreement between PCR and culture was 95%. Members of the two biovars of U. urealyticum could be distinguished by the size of the PCR UM-1 amplification products. Biovar differentiation was also demonstrated by two additional sets of PCRs: PCR UM-2 and UM-3. The PCR UM-2 was used to amplify biovar 1, while PCR UM-3 amplified biovar 2 specifically. The results indicated that use of the MB antigen gene as a target for PCR amplification could provide rapid and specific detection and biotyping of ureaplasma DNA in urogenital samples.
Teng
L J
LJ
Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, ROC.
Ho
S W
SW
Ho
H N
HN
Liaw
S J
SJ
Lai
H C
HC
Luh
K T
KT
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016990:Ureaplasma urealyticum / Q000145:classification / Q000302:isolation & purification*