title: Biodistribution of liposomes after extradural administration in rodents.
abstract: We have mapped over 24 h the biodistribution of 99mTc-labelled multilamellar and small unilamellar liposomes in rabbits and rats by scintigraphic imaging after extradural injection. Multilamellar vesicles formed a depot at the site of injection; small unilamellar vesicles spread immediately along the extradural space and entered the systemic compartment 30 min after injection. Well-delineated liver and kidney labellings were seen after 24 h. The use of 3H-cholesterol-labelled small unilamellar vesicles suggested hepatic capture of intact liposomes with sizes averaging 0.05 microns drained unmodified into the systemic circulation through the extradural lymphatics. These results have led to the selection of multilamellar vesicles (0.1-15 microns size range) for clinical trials using liposome-associated local anaesthetics.
authors.affiliation: Department of Anaesthesiology, Flemish Free University of Brussels Clinical Centre, Belgium.
authors.forename: V
authors.initials: V
authors.lastname: Umbrain
authors.forename: M
authors.initials: M
authors.lastname: Alafandy
authors.forename: P
authors.initials: P
authors.lastname: Bourgeois
authors.forename: J
authors.initials: J
authors.lastname: D'Haese
authors.forename: J G
authors.initials: JG
authors.lastname: Boogaerts
authors.forename: G
authors.initials: G
authors.lastname: Goffinet
authors.forename: F
authors.initials: F
authors.lastname: Camu
authors.forename: F J
authors.initials: FJ
authors.lastname: Legros
mesh_terms: D000818:Animals; D004337:Drug Carriers; D006321:Heart / Q000000981:diagnostic imaging; D007268:Injections, Epidural; D007668:Kidney / Q000000981:diagnostic imaging; D008081:Liposomes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D008099:Liver / Q000000981:diagnostic imaging; D011817:Rabbits; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar; D013670:Sodium Pertechnetate Tc 99m; D013997:Time Factors; D014018:Tissue Distribution; D014316:Tritium
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D014316:Tritium; D013670:Sodium Pertechnetate Tc 99m