Expression of lectin, interleukin-2 and histopathologic blood group binding sites in prostate cancer and its correlation with integrated optical density and syntactic structure analysis.
The binding of several biotinylated biologic probes was determined in sections of 20 surgical specimens of prostate cancer and of 21 biopsy specimens of hyperplastic prostate. Whereas neither the immunomodulatory, galactoside-specific lectin from Viscum album nor the human beta-galactoside-specific lectin (M(r) 14 kd) or its specific antibody discerned any remarkable differences, the lectin from Urtica dioica (UDA) and interleukin-2, the in vitro production of which is enhanced by this lectin, exhibited obvious preference for hyperplastic cells. In addition, the presence of binding sites for chemically synthesized blood group determinants was tested. Carcinoma cases revealed a higher percentage of binding of synthetic blood group trisaccharide H than hyperplasia cases. Due to these differences, diverse parameters, derived from measurement of integrated optical density (IOD) and from syntactic structure analysis, were correlated with the extent of binding of these biologic probes for the tumor cases. Primarily, parameters that are related to computation of a minimum spanning tree were significantly different in positive and negative cases for both UDA and interleukin-2. For the binding of blood group trisaccharide H the 5C exceeding rate, the 2CV deviation index and the distance of neighboring tumor cells with an IOD > 5 were clearly dissimilar. Our results thus suggest an extension of the panel of biologic probes for prostate cancer and substantiate the usefulness of correlations of binding of selected biologic probes to features derived from the assessment of IOD and syntactic structure analysis.
Kayser
K
K
Department of Pathology, Thoraxklinik, Heidelberg, Germany.
Bubenzer
J
J
Kayser
G
G
Eichhorn
S
S
Zemlyanukhina
T V
TV
Bovin
N V
NV
Andre
S
S
Koopmann
J
J
Gabius
H J
HJ
D000230:Adenocarcinoma / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D000368:Aged; D001665:Binding Sites; D015415:Biomarkers; D001789:Blood Group Antigens / Q000378:metabolism*; D002467:Cell Nucleus / Q000473:pathology; D003937:Diagnosis, Differential; D006651:Histocytochemistry; D006801:Humans; D007376:Interleukin-2 / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014784:Mistletoe; D055095:Optics and Photonics; D037121:Plant Lectins; D010946:Plants, Medicinal; D011470:Prostatic Hyperplasia / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D011994:Recombinant Proteins / Q000378:metabolism
D015415:Biomarkers; D001789:Blood Group Antigens; D007376:Interleukin-2; D037102:Lectins; D037121:Plant Lectins; D011994:Recombinant Proteins