D-lysergic acid reduces microtubule-associated tau protein in SH-SY5Y human neuroblastoma cells.
1. SH-SY5Y, an adrenergic human neuroblastoma cell line, was used to examine the hypothesis that D-lysergic acid (LSD) affects the metabolism of microtubule-associated tau protein, thus affecting microtubule assembly and the transport of neurotransmitters. 2. After 48 hr treatment LSD (10(-5) and 10(-7) M) decreased 50 kDa tau protein in the membrane (pellet) fraction. The drug (10(-5) M) also decreased in the cytoplasmic (supernatant) fraction. 3. This reduction in tau protein was accompanied by a 65% increase (P < 0.05) in total protein after LSD (10(-7) M) in the cytoplasmic fraction.
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Department of Anatomy, College of Human Medicine, Michigan State University, East Lansing 48824, USA.
D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002648:Child; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D003864:Depression, Chemical; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008237:Lysergic Acid / Q000494:pharmacology*; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009447:Neuroblastoma / Q000378:metabolism*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D016875:tau Proteins / Q000096:biosynthesis*
D009419:Nerve Tissue Proteins; D016376:Oligonucleotides, Antisense; D016875:tau Proteins; D008237:Lysergic Acid