IL-4-induced B cell migration involves transient interactions between beta 1 integrins and extracellular matrix components.
IL-4 has previously been shown to stimulate motile responses in murine B lymphocytes. This was studied as acquisition of motile morphology and migration through filters in microchemotaxis chambers. In this paper, we investigated IL-4-stimulated migration of B cells into gels of native collagen fibers, which may be a more physiologically relevant assay. When IL-4 was present in the gel and/or in the medium above, B cells were able to invade the collagen gel. Migration was dependent on the dose of IL-4 and was optimal after 45 h of incubation. It appeared that IL-4 acted by inducing both chemokinesis and chemotaxis. Fibronectin (FN) was found to be an important factor for B cell locomotion, since low concentrations of FCS or FN in the gel matrix greatly improved migration. B cell locomotion was inhibited by antibodies specific for beta 1, alpha 4 and alpha 5 integrins, indicating the presence of integrin-extracellular matrix (ECM) interactions in lymphocyte motility responses. Migration was not associated with an up-regulation of beta 1, alpha 4 or alpha 5 integrins. The adhesion between substrate and cells is likely to be of low affinity, since IL-4-stimulated, as well as non-stimulated B cells, did not adhere to ECM-coated culture wells. Our data suggest that transient interactions between integrins and the ECM matrix may favour B cell migration.
Elenström-Magnusson
C
C
Department of Cell and Molecular Biology, Medical Nobel Institute, Stockholm, Sweden.
Chen
W
W
Clinchy
B
B
Obrink
B
B
Severison
E
E
D000818:Animals; D000906:Antibodies / Q000502:physiology; D001402:B-Lymphocytes / Q000502:physiology*; D002464:Cell Migration Inhibition; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002630:Chemotactic Factors / Q000502:physiology; D003094:Collagen; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005260:Female; D005353:Fibronectins / Q000502:physiology; D005782:Gels; D019012:Integrin beta1 / Q000276:immunology / Q000378:metabolism*; D015847:Interleukin-4 / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA
D000906:Antibodies; D002630:Chemotactic Factors; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D005782:Gels; D019012:Integrin beta1; D015847:Interleukin-4; D003094:Collagen