title: Influence of the temperature upon the proliferative response of lymphocytes of tench (Tinca tinca) during winter and summer.
abstract: The influence of incubation temperature upon proliferation of Tinca tinca lymphocytes induced by phytohemagglutinin (PHA) and E. coli lipopolisaccharide (LPS) mitogens was studied during the summer and the winter. The cultures were performed in vitro at 22 degrees C in both summer and winter, and at 12 and 30 degrees C in winter and summer respectively. The proliferative response at 22 degrees C was higher than that at 12 degrees C during the winter, and a small increase was observed at 30 degrees C respective to 22 degrees C in summer. These results indicate that in vitro lymphocyte proliferation requires temperatures higher than those in the fish environment.
authors.affiliation: Departamento de Fisiología Animal, Facultad de Ciencias, Universidad de Extremadura, Badajoz, Spain.
authors.forename: M E
authors.initials: ME
authors.lastname: Collazos
authors.forename: E
authors.initials: E
authors.lastname: Ortega
authors.forename: C
authors.initials: C
authors.lastname: Barriga
mesh_terms: D000818:Animals; D003530:Cyprinidae / Q000276:immunology*; D005260:Female; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D012621:Seasons; D013696:Temperature
chemical_list: D008070:Lipopolysaccharides; D010835:Phytohemagglutinins