Bovine activin receptor type II cDNA: cloning and tissue expression.
The cDNA encoding the bovine activin type II receptor has been cloned by reverse transcription-polymerase chain reaction (RT-PCR) amplification of a bovine testicular RNA preparation. Sequence comparisons of the bovine activin type II receptor with its human, mouse and rat homologues show strong evolutionary conservation at the nucleotide level of 94.9%, 93.5%, 92.9% and at the amino acid level of 98.6%, 99.0%, 98.8%, respectively. Bovine activin type II receptor mRNA is widely but not strongly expressed in reproductive tissues, with a major RNA band at 6 kb and minor bands at 5 kb and 3 kb. The differential levels of expression observed in these tissues suggest that levels of bActRII gene expression are regulated. Furthermore, we have observed decreasing levels of the bovine activin type II receptor mRNA with testes maturation.
Ethier
J F
JF
Department of Anatomy and Physiology, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, Québec, Canada.
Houde
A
A
Lussier
J G
JG
Silversides
D W
DW
D029404:Activin Receptors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002417:Cattle; D003001:Cloning, Molecular; D003062:Codon; D018076:DNA, Complementary / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D017978:Receptors, Growth Factor / Q000737:chemistry / Q000235:genetics*; D017385:Sequence Homology; D013737:Testis / Q000737:chemistry / Q000196:embryology
D003062:Codon; D018076:DNA, Complementary; D012333:RNA, Messenger; D017978:Receptors, Growth Factor; D029404:Activin Receptors; D012194:RNA-Directed DNA Polymerase