The SS-A/SS-B autoantigenic complex: localization and assembly.
The subcellular distribution of Ro ribonucleoprotein particles (Ro RNPs) and their constituents was determined using mammalian tissue culture cells and Xenopus laevis oocytes. Cell fractionation revealed that Ro RNPs are restricted to the cytoplasm, while the Ro RNP-specific proteins were also present in the nucleus. Transport studies indicated that the protein components of Ro RNPs are actively imported into the nucleus while the RNA component accumulates in the cytoplasm. These results suggest a model for the assembly of Ro RNPs.
Peek
R
R
Department of Biochemistry, University of Nijmegen, The Netherlands.
van Venrooij
W J
WJ
Simons
F
F
Pruijn
G
G
D000818:Animals; D001324:Autoantigens / Q000032:analysis* / Q000276:immunology* / Q000378:metabolism; D001327:Autoimmune Diseases / Q000276:immunology* / Q000378:metabolism; D015152:Blotting, Northern; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007424:Intracellular Fluid / Q000276:immunology*; D017136:Ion Transport; D051379:Mice; D008845:Microinjections; D009865:Oocytes / Q000276:immunology / Q000378:metabolism; D012313:RNA / Q000032:analysis*; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D014982:Xenopus laevis; D000097585:SS-B Antigen; D000099207:SS-A Antigen
D001324:Autoantigens; D012313:RNA; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D000097585:SS-B Antigen; D000099207:SS-A Antigen; C116006:RO60 protein, human