Sphingosine activates cellular diacylglycerol kinase in intact Jurkat cells, a human T-cell line.
Sphingosine is known to regulate a variety of cellular functions through protein kinase C-dependent or independent pathways. In an attempt to investigate differential functions of diacylglycerol kinase (DGK) isozymes, we tested the effect of sphingosine on DGK operating in intact Jurkat cells, a human T-cell line. We found that phosphatidic acid (PA) synthesized from endogenous diacylglycerol (DG) and exogenously added short-chain DGs like dioctanoylglycerol were markedly enhanced by approx. 20 microM sphingosine. Further studies such as the use of protein kinase C down-regulated cells, mass measurements of cellular DGs, analysis of molecular species of PA and the effect of exogenous DG on the conversion of endogenous DG to PA suggested that sphingosine directly activated cellular DGK having a broad specificity toward DG molecular species.
Yamada
K
K
Department of Biochemistry and Pharmacology, School of Medicine, Sapporo Medical University, Japan.
Sakane
F
F
Imai
S
S
Takemura
H
H
D000001:Calcimycin / Q000494:pharmacology; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D019852:Diacylglycerol Kinase; D004075:Diglycerides / Q000378:metabolism / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D010712:Phosphatidic Acids / Q000096:biosynthesis; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism*; D011493:Protein Kinase C / Q000172:deficiency; D013110:Sphingosine / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000201:enzymology
D004075:Diglycerides; D007527:Isoenzymes; D010712:Phosphatidic Acids; D003208:Concanavalin A; D000001:Calcimycin; D017853:Phosphotransferases (Alcohol Group Acceptor); D019852:Diacylglycerol Kinase; D011493:Protein Kinase C; D013110:Sphingosine