T cell receptor beta chain gene rearrangement and selection during thymocyte development in adult mice.
The identification is made in normal mice of the stages in T cell development at which the rearranged beta chain of the T cell receptor (TCR) is utilized to promote T cell maturation, independent of the TCR alpha chain. In addition, evidence is provided that utilization of beta chains in T cell progenitors does not preclude differentiation to TCR gamma delta + T cells. This is consistent with the view that an initial consequence of beta chain expression by early thymocytes is clonal expansion, increasing the size of the pool of useful precursors. This allows the proposal to be made that allelic exclusion may be a byproduct of cell cycle regulation during early thymocyte differentiation, which may in turn explain why the efficiency of allelic exclusion varies at different TCR or immunoglobulin loci.
Dudley
E C
EC
Department of Biology, Yale University, New Haven, Connecticut 06511.
Petrie
H T
HT
Shah
L M
LM
Owen
M J
MJ
Hayday
A C
AC
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D018960:Hyaluronan Receptors; D051379:Mice; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D002352:Carrier Proteins; D017931:DNA Primers; D018960:Hyaluronan Receptors; D016692:Receptors, Antigen, T-Cell, gamma-delta; D011956:Receptors, Cell Surface; D015375:Receptors, Interleukin-2; D016201:Receptors, Lymphocyte Homing; D004247:DNA