Complete sequence of the 43-kb human ribosomal DNA repeat: analysis of the intergenic spacer.
We have sequenced the remaining 12.4 kb of the 30-kb human ribosomal DNA intergenic spacer (IGS), which allows us to piece together both a complete IGS and a 43-kb rDNA unit. The sequence of the complete IGS reveals a collection of sequence motifs that can be correlated with functions known or expected to reside in the rDNA repeat: modulation of transcription, recombination, initiation of DNA replication, and chromosomal organization. Finally, we find that IGS accumulates variation at a much higher rate than the transcribed regions. This finding leads us to correlate sequence character with types of mutation and sequence context with rate of mutation.
Gonzalez
I L
IL
Department of Pathology, Hahnemann University, Philadelphia, Pennsylvania 19102, USA.
Sylvester
J E
JE
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000737:chemistry; D004252:DNA Mutational Analysis; D004275:DNA, Ribosomal / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D018626:Retroelements
D004275:DNA, Ribosomal; D018626:Retroelements; D004247:DNA