Natural killer (NK) and lymphokine-activated killer (LAK) activities in a patient who recovered from cancer, and the characteristics of LAK cells generated from CD4-CD8- and CD8+ peripheral blood lymphocytes.
The natural killer (NK) and lymphokine-activated killer (LAK) activities of a patient who recovered from bladder transitional cell carcinoma were determined. The patient was diagnosed in 1980 and repeated several therapies until 1986. Since 1987, he has been in a tumor-free state. Recently, very high NK and LAK activities were found. The NK activity was recognized not only against the NK-sensitive cell line K562, but also against NK-unsensitive Raji and Daudi cell lines and against autotumor cell line H41. The percentage of CD16+ in peripheral blood lymphocytes (PBL) was 52.9%, the CD8+CD16+ was 26.3%, and the CD4-CD8-CD16+ was 26.6% (17.5, 5.4 and 12.1% in healthy blood donor (control), respectively). Because the patient had large numbers of CD4-CD8- cells in the PBL, CD4-CD8- LAK were generated from them. The CD4-CD8- LAK cells exhibited extremely high killer activity, whereas the CD8+ LAK cells generated from the CD8+ cells showed a low level of killer activity. These results suggest that the tumor-free condition of this patient may be related to these high killer activities. In this patient, several cytokines were also determined, although the results were not completely interpreted.
Komatsu
F
F
Blood Transfusion Service, School of Medicine, Tokyo Medical & Dental University, Japan.
Kihara
K
K
D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002295:Carcinoma, Transitional Cell / Q000276:immunology*; D016207:Cytokines / Q000097:blood; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007111:Immunity, Cellular; D007113:Immunity, Innate; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D013997:Time Factors; D001749:Urinary Bladder Neoplasms / Q000276:immunology*
D016207:Cytokines