title: Trisomy 4 in 'stem cell-like' leukemic cells of a patient with AML.
abstract: CD34 positive progenitor cells were analyzed in the bone marrow aspirate from a patient with newly diagnosed AML FAB M4. The patient had trisomy 4 as sole cytogenetic abnormality and a dominant population of CD34 negative leukemic blasts. Karyotyping of the FACS isolated, minor subpopulation of CD34+/CD38-, 'stem cell'-like cells (incidence 0.29%) revealed trisomy 4 in 11/13 metaphases. No metaphases were obtained in the CD34 negative subpopulation. The experiments point to the existence of leukemic stem cells in the CD34+/CD38- compartment in AML patients with trisomy 4.
authors.affiliation: Department of Internal Medicine, Georg-August-Universität, Göttingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Feuring-Buske
authors.forename: D
authors.initials: D
authors.lastname: Haase
authors.forename: S
authors.initials: S
authors.lastname: Könemann
authors.forename: C
authors.initials: C
authors.lastname: Troff
authors.forename: D
authors.initials: D
authors.lastname: Grove
authors.forename: W
authors.initials: W
authors.lastname: Hiddemann
authors.forename: B
authors.initials: B
authors.lastname: Wörmann
mesh_terms: D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D000943:Antigens, Differentiation / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology; D002871:Chromosome Banding; D002894:Chromosomes, Human, Pair 4; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D015479:Leukemia, Myelomonocytic, Acute / Q000473:pathology*; D008562:Membrane Glycoproteins; D009699:N-Glycosyl Hydrolases / Q000032:analysis; D014314:Trisomy
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D000943:Antigens, Differentiation; D008562:Membrane Glycoproteins; D009699:N-Glycosyl Hydrolases; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1