Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes.
1. We describe the isolation of viable merozoites from erythrocytes infected with Babesia bovis or Babesia bigemina organisms by ammonium chloride lysis. 2. Parasite morphology was examined by both light and transmission electron microscopy. Erythrocyte-free parasites maintain their viability and infectivity, retain their antigenicity and are suitable for use in the indirect fluorescent antibody assay.
Machado
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RZ
Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, UNESP, Jaboticabal, SP, Brasil.
Valadão
C A
CA
Melo
W R
WR
Alessi
A C
AC
D000643:Ammonium Chloride; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D001403:Babesia / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D016793:Babesia bovis / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D002417:Cattle; D004912:Erythrocytes / Q000469:parasitology*; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron
D000913:Antibodies, Protozoan; D000643:Ammonium Chloride