The expression of carbonic anhydrases II and IV in the human pancreatic cancer cell line (Capan 1) is associated with bicarbonate ion channels.
Human pancreatic ductal cells of the Capan 1 cell line differentiate progressively during growth. After the exponential growth phase, the cells elongate and become polarized with their apical poles covered by microvilli and separated from the basolateral pole by tight junctions. In this stationary phase, they form domes, which are thought to result from the exchange of water and electrolytes. In this study, we demonstrated, using patch-clamp techniques, that HCO3- ions exit via the g350 high conductance anionic channel we observed recently at the Capan 1 cell surface. This g350 channel was thought to be either a Cl-/HCO3- antiport or a simple HCO3- channel. The stilbene derivatives 4-acetamido-4 isothiocyano-2-2'-disulfonic acid (SITS) and 4,4' diisothiocyano stilbene-2,2' disulfonic acid (DIDS) reduced both the number of domes and the Cl- and HCO3- flux through the g350 channel. Moreover, using histochemical, immunocytochemical and biochemical methods we showed that Capan 1 cells express a specific pattern of carbonic anhydrases (CA). Two types of CA were detected: the CA II isozyme mainly localized in the cytoplasm, but also found beneath the inner leaflet of the apical plasma membrane, and the CA IV isozyme localized on the outer leaflet of the apical plasma membrane and microvilli. Their molecular masses were 30 (CA II) and 55 kDa (CA IV), respectively. They were expressed continuously during the exponential growth phase, although their activity increased greatly during the stationary phase. Inhibition of dome formation by acetazolamide indicated the existence of a direct relationship between dome formation and CA. Characteristic structures with a central electron-dense core surrounded by a light halo were observed on the surface of cell membrane using histochemical and immunocytochemical methods. These structures were thought to represent a channel, corresponding possibly to CA IV. Our observations suggest that Capan 1 cells, despite their neoplasic transformation, produce HCO3- ions in the same way as normal human pancreatic ductal cells. Capan 1 cells in culture may therefore represent a suitable model for studying pancreatic duct HCO3- secretion at the cellular and molecular levels.
Mahieu
I
I
Laboratoire de Biologie Cellulaire, UPS, Toulouse, France.
Becq
F
F
Wolfensberger
T
T
Gola
M
M
Carter
N
N
Hollande
E
E
D000086:Acetazolamide / Q000494:pharmacology; D001639:Bicarbonates / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000032:analysis / Q000096:biosynthesis*; D002455:Cell Division; D006801:Humans; D007150:Immunohistochemistry; D007473:Ion Channels / Q000378:metabolism*; D007527:Isoenzymes; D008854:Microscopy, Electron; D010190:Pancreatic Neoplasms / Q000378:metabolism* / Q000648:ultrastructure; D018408:Patch-Clamp Techniques; D014407:Tumor Cells, Cultured
D001639:Bicarbonates; D007473:Ion Channels; D007527:Isoenzymes; D002256:Carbonic Anhydrases; D000086:Acetazolamide