Immunochemistry of group A and Inaba C antigen factors constituting the O antigen of O1 Vibrio cholerae.
Serological cross-reactivity among intact lipopolysaccharides (LPS) from O1 Vibrio cholerae Inaba O-form (Inaba), Yersinia enterocolitica O9 (O9), non-O1 V. cholerae serogroup Hakata (Hakata) and Vibrio bio-serogroup 1875 Variant (1875 Variant) (all of which share Inaba antigen factor C), as well as a total of six kinds of chemically modified LPS (three from O9 and three from Inaba) was demonstrated by passive hemolysis and passive hemolysis inhibition by using these LPS as antigen for sensitizing sheep red blood cells and as inhibitor. These intact as well as chemically modified LPS contained, in their O polysaccharide chain, alpha(1-->2)-linked linear perosamine (4-amino-4,6-dideoxy-D-manno-pyranose) homopolymers with different N-acyl groups: their acyl groups comprise 3-deoxy-L-glycero-tetronyl (Inaba LPS), formyl (O9 LPS), 3-hydroxypropionyl (1875 Variant LPS), acetyl (Hakata LPS and artificially introduced into Inaba and O9 LPS), propionyl and butyryl (both artificially introduced into Inaba and O9 LPS) groups. N-Deacylation of the alpha(1-->2)-linked N-(3-deoxy-L-glycero-tetronyl)perosamine homopolymer of Inaba and the N-formyl one of O9 LPS resulted in virtual elimination of their serological reactivity with both homologous and heterologous antisera. Furthermore, when the resultant NH2 groups of the N-deacylated perosamine homopolymers of both LPS were N-acylated with acetyl, propionyl or butyryl groups, they markedly recovered both of their serological reactivities. These results are compatible with the interpretation that the Inaba antigen factor C possessed by the four bacteria is substantially related to the common presence of N-acyl groups, regardless of their identity, residing in the perosamine residues constituting the O polysaccharide chain of their LPS. It was also indicated that the group antigen factor A of O1 V. cholerae is substantially related to the 3-deoxy-L-glycero-tetronyl groups residing in the perosamine homopolymer of Inaba LPS.
Isshiki
Y
Y
Department of Microbiology, School of Pharmaceutical Sciences Josai University, Saitama, Japan.
Haishima
Y
Y
Kondo
S
S
Hisatsune
K
K
D000907:Antibodies, Bacterial / Q000276:immunology; D001681:Biological Assay; D002241:Carbohydrates / Q000737:chemistry; D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006461:Hemolysis; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D019081:O Antigens; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000276:immunology; D014734:Vibrio cholerae / Q000276:immunology*
D000907:Antibodies, Bacterial; D002241:Carbohydrates; D008070:Lipopolysaccharides; D019081:O Antigens; D011135:Polysaccharides, Bacterial