[Detection of amplified DNA sequences by comparative genomic in situ hybridization with human glioma tumor DNA as probe].
Comparative genomic hybridization (CGH) provides a new possibility for the investigation of genetic alterations in tumour genomes. In our experiments CGH was carried out using genomic DNA from human glioblastoma multiforme (GBM) as a probe for chromosomal in situ suppression hybridization. Amplified DNA sequences contained in the tumour DNA showed specific signals, revealing the chromosomal positions of these sequences. Using this approach we detected amplifications of different chromosomal segments in individual GBM specimens. In accordance with the results from Southern analysis demonstrating amplification of the EGFR gene in 45% of human GBM, CGH signals in different GBM mapped to the region of this gene on chromosome 7p. Other signals detected by CGH involved chromosome 12q and 8q. Our data demonstrate CGH as a novel comprehensive and rapid approach for the analysis of complex genomic alterations in glial tumours.
Schlegel
J
J
Institut für Pathologie, Universität Regensburg.
Scherthan
H
H
Arens
N
N
Stumm
G
G
Cremer
T
T
Kiessling
M
M
D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D002897:Chromosomes, Human, Pair 7; D002898:Chromosomes, Human, Pair 8; D015342:DNA Probes; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics*; D066246:ErbB Receptors / Q000235:genetics; D005260:Female; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D017403:In Situ Hybridization; D007621:Karyotyping; D008297:Male; D008677:Metaphase
D015342:DNA Probes; D004273:DNA, Neoplasm; D066246:ErbB Receptors