Down-regulation of CFTR expression in an HT29-derived cell line by stable antisense RNA production.
In order to examine the interactions of the cAMP activated CFTR chloride ion channel with other chloride ion channels present in epithelial cells we have generated a model based on antisense mRNA down regulation of CFTR expression. The HT29-derived epithelial cell line CJC4-1 has integrated a plasmid that is constitutively expressing CFTR exons 1-6 in the antisense orientation, from the MoMULV LTR. This cell line shows a reduction in cAMP-activated chloride efflux, as measured by iodide efflux assay, in comparison to the parental HT29 cell line and a sense transfected control.
Wardle
C J
CJ
Institute of Molecular Medicine, Oxford University, John Radcliffe Hospital, UK.
Harris
A
A
D002460:Cell Line; D002470:Cell Survival; D018118:Chloride Channels / Q000032:analysis*; D003550:Cystic Fibrosis / Q000235:genetics; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D015536:Down-Regulation; D004848:Epithelium / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D016372:RNA, Antisense / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection
C505032:CFTR protein, human; D018118:Chloride Channels; D008565:Membrane Proteins; D016372:RNA, Antisense; D012333:RNA, Messenger; D019005:Cystic Fibrosis Transmembrane Conductance Regulator