Effects of persistent chlorinated hydrocarbons on fertility and embryonic development in the rabbit.
The commercial polychlorinated biphenyl (PCB) formulation Aroclor 1260 (4 mg/kg body weight), technical grade dichlorodiphenyltrichloroethane (DDT; 3 mg) and Lindane (gamma-hexachlorocyclohexane; 0.8 mg) were administered orally, either separately or in combination, to sexually mature female rabbits three times per week for 12-15 weeks. Oviductal and uterine luminal fluid, cleavage stage embryos (day 1 post coitum), blastocysts (day 6), fetuses, exocoelic fluid and placentae (day 11) were analysed, firstly for chlorinated hydrocarbon residues, and secondly for embryonic and fetal development. The doses applied were well tolerated by the treated animals. PCB and DDT accumulated in uterine secretions (day 6) but not in oviductal luminal fluid (day 1). Both chlorinated hydrocarbons were found in preimplantation blastocysts. Residues in day 11 fetuses were 16- (DDT) or 18-fold (PCB) higher than in day 6 blastocysts. Significant amounts were also detected in placental tissue and in exocoelic fluid. A specific accumulation of the highly chlorinated biphenyl congener no. 180 was noted in fetuses, placentae and exocoelic fluid. The clear accumulation of the chlorinated hydrocarbon compounds in luminal fluid and embryonic tissue is contrasted by rather weak effects on fertility. No statistically significant differences between treated animals and controls were observed for fertilization rate and pre- and post-implantation (up to day 11 post coitum) losses. However, in females exposed to PCB, a 20% higher loss of blastocysts was noticed, as compared with controls (P > 0.05). This effect was shown on day 6 of embryonic development and may be due to the embryotoxic activities of PCB.
Seiler
P
P
Department of Anatomy and Reproductive Biology, School of Medicine, RWTH University of Aachen, Germany.
Fischer
B
B
Lindenau
A
A
Beier
H M
HM
D000818:Animals; D001140:Aroclors / Q000493:pharmacokinetics / Q000494:pharmacology*; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology; D002970:Cleavage Stage, Ovum / Q000187:drug effects; D003634:DDT / Q000493:pharmacokinetics / Q000494:pharmacology*; D010064:Embryo Implantation / Q000187:drug effects; D047108:Embryonic Development / Q000187:drug effects; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005298:Fertility / Q000187:drug effects*; D001556:Hexachlorocyclohexane / Q000493:pharmacokinetics / Q000494:pharmacology*; D010920:Placenta / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D011817:Rabbits; D014599:Uterus / Q000378:metabolism
D001140:Aroclors; C026987:aroclor 1260; D001556:Hexachlorocyclohexane; D003634:DDT