Structural studies of the O-antigen oligosaccharides from two strains of Moraxella catarrhalis serotype C.
The oligosaccharide parts from Moraxella (Branhamella) catarrhalis serotype C lipooligosaccharides were isolated by mild acid hydrolysis followed by gel permeation chromatography. Four different oligosaccharides could be identified from strain RS26 and two from strain RS10. The structures of the O-oligosaccharides were established by methylation analyses, mass spectrometry, and NMR spectroscopy. It is concluded that the oligosaccharide O-antigens from RS26 are a mixture of octa-, deca-, and undeca-saccharides, and most likely a heptasaccharide. Strain RS10 contains the deca- and the undeca-saccharide only. The structures for the oligosaccharides are shown below. [formula: see text] OS(7) [formula: see text] OS(8) [formula: see text] OS(10) [formula: see text] OS(11) Methylation analysis of the intact lipooligosaccharides showed that two Kdo residues were present, one terminal and one 4,5-substituted residue. It also showed that they consisted of a lipid A portion with 6-substituted glucosamine residues.
Edebrink
P
P
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
Jansson
P E
PE
Rahman
M M
MM
Widmalm
G
G
Holme
T
T
Rahman
M
M
D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008969:Molecular Sequence Data; D001936:Moraxella catarrhalis / Q000737:chemistry* / Q000276:immunology; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial