title: The primary structure of a basic (pI 9.0) fatty acid-binding protein from liver of Gallus domesticus.
abstract: The complete amino acid sequence of a basic (pI 9.0) fatty acid-binding protein purified from liver of Gallus domesticus was determined by automated Edman degradation of tryptic, CNBr/HFBA and Staphylococcus aureus protease peptides. The protein contains 125 amino acid residues which correspond to a molecular mass of 14094. The identification of the blocked N-terminus Ac-Ala required digestion of a SV-8 peptide with the acylamino acid-releasing enzyme prior to sequence analysis. Sequence comparison shows that chicken liver basic-FABP has a significant similarity to other proteins belonging to the superfamily of intracellular lipid molecule binding proteins. Moreover, these sequence data confirm that basic-FABP probably binds its substrate in a slightly different way when compared with other FABPs. Basic-FABP was submitted to the EMBL Data Library with an accession number of P80226.
authors.affiliation: Istituto di Fisiologia Veterinaria e Biochimica, Università di Milano, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Ceciliani
authors.forename: H L
authors.initials: HL
authors.lastname: Monaco
authors.forename: S
authors.initials: S
authors.lastname: Ronchi
authors.forename: L
authors.initials: L
authors.lastname: Faotto
authors.forename: P
authors.initials: P
authors.lastname: Spadon
mesh_terms: D000468:Alkalies; D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry*; D002645:Chickens / Q000378:metabolism*; D050556:Fatty Acid-Binding Proteins; D008099:Liver / Q000737:chemistry*; D008969:Molecular Sequence Data; D018996:Myelin P2 Protein / Q000737:chemistry*; D009363:Neoplasm Proteins
chemical_list: D000468:Alkalies; D002352:Carrier Proteins; D050556:Fatty Acid-Binding Proteins; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins