title: Quantitation, cellular localization and regulation of neurokinin receptor gene expression within the rat substantia nigra.
abstract: The diverse biological effects of substance P and related peptides are mediated by multiple neurokinin receptors. The CNS sites of neurokinin receptor biosynthesis have not been fully elucidated and little is known about the regulation of neurokinin receptor gene expression. In the present study, the abundance of neurokinin-1, neurokinin-2 and neurokinin-3 receptor messenger RNAs in various rat brain regions was quantitated using a sensitive solution hybridization assay. Midbrain neurokinin receptor gene expression was then examined in detail. In situ hybridization experiments localized high levels of neurokinin-3 receptor messenger RNA to presumptive dopamine neurons, as evidenced by sensitivity to 6-hydroxydopamine lesions and the presence of tyrosine hydroxylase messenger RNA in serial sections. Lesions of nigral afferent (including substance P-containing) pathways from the caudate-putamen increased both nigral neurokinin-3 and neurokinin-1 receptor messenger RNA levels two- to three-fold. These data provide the anatomical substrate for physiological data suggesting that substance P (released from striatonigral neurons) may act on nigral cells through neurokinin-1 receptors, while the substance P co-transmitter neurokinin A may act preferentially on dopamine neurons through neurokinin-3 receptors. The magnitude of denervation-induced changes in neurokinin receptor messenger RNAs suggests significant plasticity of neurokinin receptor gene expression.
authors.affiliation: Department of Psychiatry (Cellular and Clinical Neurobiology), Wayne State University School of Medicine, Detroit, MI 48201, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Whitty
authors.forename: P D
authors.initials: PD
authors.lastname: Walker
authors.forename: D J
authors.initials: DJ
authors.lastname: Goebel
authors.forename: M S
authors.initials: MS
authors.lastname: Poosch
authors.forename: M J
authors.initials: MJ
authors.lastname: Bannon
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D008297:Male; D016627:Oxidopamine / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D018040:Receptors, Neurokinin-1 / Q000235:genetics*; D018041:Receptors, Neurokinin-2 / Q000235:genetics*; D018042:Receptors, Neurokinin-3 / Q000187:drug effects / Q000235:genetics*; D013373:Substance P / Q000502:physiology; D013378:Substantia Nigra / Q000187:drug effects / Q000502:physiology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D018040:Receptors, Neurokinin-1; D018041:Receptors, Neurokinin-2; D018042:Receptors, Neurokinin-3; D013373:Substance P; D016627:Oxidopamine; D014446:Tyrosine 3-Monooxygenase