Secretion of streptokinase fusion proteins from Escherichia coli cells through the hemolysin transporter.
The hemolysin (HlyA) secretion system was used to achieve the sec-independent secretion of streptokinase (Skc) originating from Streptococcus equisimilis into the medium by Escherichia coli cells. The in-frame fusions of the skc gene, either possessing or lacking a region encoding the signal peptide (SP) with the 3'-end of the hlyA gene of various lengths were analysed. All hybrids retained Skc activity. Hybrid proteins devoided of the N-terminal SP, regardless of length of the hlyA secretion signal (62 vs. 194 amino acids), were secreted into the medium by the E. coli HlyA transporter at similar levels. Considerable amounts of hybrid proteins were still, however, associated with E. coli cells, mainly in the degraded form.
Kern
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I
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa.
Cegłowski
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P
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D001427:Bacterial Toxins / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005341:Fibrinolysin / Q000378:metabolism; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D010958:Plasminogen / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D013291:Streptococcus / Q000201:enzymology; D013300:Streptokinase / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; C050837:Hlyb protein, Bacteria; D011993:Recombinant Fusion Proteins; D010958:Plasminogen; D001616:beta-Galactosidase; D013300:Streptokinase; D005341:Fibrinolysin