title: Effects of bradykinin and bradykinin analogues on spleen cells of mice.
abstract: The present study was undertaken to examine the effects of bradykinin and selected bradykinin analogues on mononuclear cells derived from mouse spleen. Bradykinin as well as des-Arg9-bradykinin, a bradykinin B1 receptor agonist, were able to induce the release of so-called charge-changing lymphokines, which could be identified as interleukin-1, interleukin-6, interleukin-2 and as interleukin-2 receptor. The cytokine release evoked by bradykinin and all analogues showed a bell-shaped dose dependence in a range of 10(-8) M to 10(-6) M and could be inhibited by the specific bradykinin receptor antagonist, D-Arg0[Hyp3,Thi5,D-Tic7,Oic8]bradykinin (HOE140), and by bradykinin analogues with N-methyl-phenylalanine at position 2 in concentrations as low as 10(-12) M and 10(-13) M, respectively. Obviously the N-terminus of bradykinin seems to be responsible for the interaction with the mononuclear cells concerning all peptides investigated.
authors.affiliation: Institute of Pharmacology and Toxicology, Medical Faculty, University of Rostock, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Paegelow
authors.forename: H
authors.initials: H
authors.lastname: Werner
authors.forename: S
authors.initials: S
authors.lastname: Reissmann
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D065168:Bradykinin Receptor Antagonists; D004586:Electrophoresis; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects; D007124:Immunoenzyme Techniques; D007375:Interleukin-1 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism; D012756:Sheep; D013154:Spleen / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D065168:Bradykinin Receptor Antagonists; D007375:Interleukin-1; D007376:Interleukin-2; D015850:Interleukin-6; D001920:Bradykinin