Expression of V beta gene segments by Sezary cells.
The T-cell receptor V beta repertoire expressed by Sezary cells was determined in a series of 16 patients whose samples have been shown to contain a majority of tumor cells. By using anti-V beta monoclonal antibodies, polymerase chain reaction analysis of expressed V beta, and, in selected cases, nucleotide sequencing, we have shown that the expressed V beta segments belong to five V beta families (V beta 5, V beta 6, V beta 8, V beta 13, and V beta 18), which contain a large fraction of the T-cell receptor V beta repertoire and do not share significant similarities in complementary determining region 4. V beta segments from these five families were also found to be strongly expressed by CD4 + CD7- peripheral blood cells obtained by fluorescence-activated cell sorting from two healthy donors. The diversity of the V beta repertoire expressed by Sezary cells appears to be similar to that expressed by circulating non-neoplastic T cells. These data do not support the hypothesis that a common superantigen is involved in the initiation of this form of cutaneous T-cell lymphoma.
Gorochov
G
G
Laboratory of Molecular Hematology, Centre Hayem, St. Louis Hospital, Paris, France.
Bachelez
H
H
Cayuela
J M
JM
Legac
E
E
Laroche
L
L
Dubertret
L
L
Sigaux
F
F
D015703:Antigens, CD / Q000032:analysis; D019011:Antigens, CD7; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D001483:Base Sequence; D015704:CD4 Antigens / Q000032:analysis; D006801:Humans; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D012751:Sezary Syndrome / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D012878:Skin Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D018089:Superantigens / Q000502:physiology
D015703:Antigens, CD; D019011:Antigens, CD7; D000945:Antigens, Differentiation, T-Lymphocyte; D015704:CD4 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens