Grafting peptides onto polystyrene microplates for ELISA.
Three peptides corresponding respectively to two Epstein-Barr viral epitopes and to the c-erbB-2 oncogene product were synthesized with the aim of developing an immunoenzymatic assay. Preliminary experiments indicated that the efficiency of the assay was profoundly affected by the nature of the solid phase for each peptide. In order to optimize the assay the three peptides were covalently coupled to functionalized polystyrene microplates which were used to immobilize both haptens and nucleic acids in a previous study. The results obtained indicate that the use of the carboxylated surfaces permits the linking strategy to be adapted to each peptide. Moreover, high sensitivities (5 x 10(-10)-1 x 10(-13) M) were obtained using amounts of the peptides much lower than those used in the standard system.
Niveleau
A
A
URA 1459, CNRS, Institut Pasteur de Lyon, France.
Bruno
C
C
Drouet
E
E
Brebant
R
R
Sergeant
A
A
Troalen
F
F
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D000939:Epitopes / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology; D007158:Immunologic Techniques / Q000295:instrumentation / Q000706:statistics & numerical data; D051379:Mice; D008969:Molecular Sequence Data; D010455:Peptides / Q000235:genetics / Q000276:immunology*; D011137:Polystyrenes; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D010455:Peptides; D011137:Polystyrenes; D018719:Erb-b2 Receptor Tyrosine Kinases