Activity of a monoclonal antibody against Besnoitia besnoiti endozoites.
Besnoitia besnoiti multiplication in vitro was inhibited by a specific organelle complex-directed monoclonal antibody (MoAb) raised against the endozoite stage. Multiplication rates in antibody-treated cultures were lower at parasite/cell ratios of 1:1, 1:2 and 1:10, than in untreated cultures, after 4 or 8 d of incubation. At 1:100 ratio, there was no difference between test and control cultures irrespective of the incubation period. In in vivo experiments, the anti-B besnoiti MoAb had no neutralizing effect on the infectivity of endozoites. Inoculation of gerbils with antibody-preincubated endozoites, followed by treatment with these MoAb through 5 successive days ultimately failed to prevent death in any of them. In Western blots the specific anti-B besnoiti MoAb of the IgG1 subclass recognized a 70 kDa endozoite protein in the cytosolic and the insoluble membrane fraction of endozoites.
Shkap
V
V
Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel.
Pipano
E
E
Zwernemann
B
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000913:Antibodies, Protozoan / Q000276:immunology*; D000953:Antigens, Protozoan / Q000276:immunology; D002455:Cell Division; D002522:Chlorocebus aethiops; D003048:Coccidiosis / Q000276:immunology* / Q000517:prevention & control; D021741:Eimeriida / Q000254:growth & development / Q000276:immunology*; D005849:Gerbillinae; D014709:Vero Cells
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan