The use of Lucifer Yellow and Mini-Ruby for intracellular staining in fixed brain tissue: methodological considerations evaluated in rat and human autopsy brains.
The quality of intracellular filling of Mini-Ruby (MR) is comparable to that of Lucifer Yellow (LY) in both perfusion-fixed rat and immersion-fixed autopsy human tissue. In adult human brain, the use of MR avoids the co-conversion of the typical intracellular lipofuscin deposits as is invariably the case during the photoconversion of LY. In autopsy studies (n = 35), the quality of intracellular staining does not correlate with age (range: 25-91 years) or the postmortem delay (range: 7-50 h). A short agonal status, however, is advantageous.
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Zentrum der Morphologie, J.W. Goethe-Universität, Frankfurt am Main, Germany.
Diekmann
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D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology; D000818:Animals; D001344:Autopsy / Q000379:methods*; D001710:Biotin / Q000031:analogs & derivatives*; D001921:Brain / Q000033:anatomy & histology*; D003911:Dextrans; D005260:Female; D005456:Fluorescent Dyes; D006801:Humans; D007546:Isoquinolines; D008062:Lipofuscin; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000648:ultrastructure; D010477:Perfusion; D011180:Postmortem Changes; D051381:Rats; D017207:Rats, Sprague-Dawley; D012235:Rhodamines; D013194:Staining and Labeling; D016707:Tissue Fixation
D003911:Dextrans; D005456:Fluorescent Dyes; D007546:Isoquinolines; D008062:Lipofuscin; D012235:Rhodamines; C081276:Miniruby; D001710:Biotin; C017475:lucifer yellow