title: Interferon and antiallergic drug regulation of histamine and tumor necrosis factor-alpha in rat mast cell subsets.
abstract: We have further characterized the heterogeneity of mast cells (MCs) by comparing the ability of rat peritoneal MCs (PMCs) and intestinal mucosal MCs (IMMCs) to produce tumor necrosis factor (TNF)-alpha and by investigating its regulation by interferon (IFN) and the antiallergic drugs nedocromil sodium (NED) and sodium cromoglycate (SCG). Although IMMCs store less TNF-alpha than PMCs, they produced comparable amounts of TNF-alpha in cytotoxic assays. Just as SCG and NED inhibit histamine secretion from PMCs but not IMMCs, IFN exhibited a similar differential effect on histamine release from these cells. However, SCG, NED, and IFN inhibit TNF-alpha-dependent cytotoxicity by both PMCs and IMMCs and reduce the steady-state levels of mRNA for TNF-alpha in PMCs. Thus, the modulation of MC mediator release depends upon the MC population and mediator studied. The inhibitory effect of SCG and NED on TNF-alpha release from MCs may explain some of their anti-inflammatory and therapeutic effects.
authors.affiliation: Department of Medicine, University of Alberta, Edmonton, Canada.
authors.forename: E Y
authors.initials: EY
authors.lastname: Bissonnette
authors.forename: J A
authors.initials: JA
authors.lastname: Enciso
authors.forename: A D
authors.initials: AD
authors.lastname: Befus
mesh_terms: D000818:Animals; D004205:Cromolyn Sodium / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D007372:Interferons / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000166:cytology; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D017835:Nedocromil / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D014409:Tumor Necrosis Factor-alpha; D017835:Nedocromil; D007372:Interferons; D004205:Cromolyn Sodium