Lipids and proteins in the Rathke's gland secretions of the North American mud turtle (Kinosternon subrubrum).
Lipids and proteins in the Rathke's gland secretions of the North American mud turtle (Kinosternon subrubrum, Kinosternidae) were analyzed by gas chromatography-mass spectrometry (GC-MS) and SDS-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. Analysis by GC-MS indicates 2,3-dihydroxypropanal and C3-C24 free or esterified fatty acids. Analysis by SDS-PAGE indicates a major protein component with an approximate molecular mass of 60 kDa and minor components ranging from ca. 23 to 34 kDa. The major component of K. subrubrum glandular secretions exhibits a mobility that matches that of the Kemp's ridley sea turtle (Lepidochelys kempi, Cheloniidae), suggesting that these proteins are evolutionarily conserved.
Seifert
W E
WE
University of Texas Medical School at Houston 77225, USA.
Gotte
S W
SW
Leto
T L
TL
Weldon
P J
PJ
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D008055:Lipids / Q000032:analysis*; D008297:Male; D008970:Molecular Weight; D009656:North America; D011506:Proteins / Q000032:analysis*; D014426:Turtles / Q000378:metabolism*
D008055:Lipids; D011506:Proteins