Analysis of human immunodeficiency virus type 1 integrase mutants.
The human immunodeficiency virus type-1 (HIV-1) integrase protein (IN) mediates the insertion of linear double-stranded viral DNA into the host genome. Mutations in IN can have different effects on the virus life cycle. In this study, Gag-Pol polyprotein processing, Tat synthesis, and viral replication were investigated in integrase-defective HIV-1 mutants. In the absence of IN synthesis, the Gag-Pol polyprotein stability, packaging, and/or processing was reduced. There was limited expression of Tat observed in IN mutants, but no viral replication.
Ansari-Lari
M A
MA
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Donehower
L A
LA
Gibbs
R A
RA
D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D017931:DNA Primers; D003673:Defective Viruses / Q000201:enzymology / Q000235:genetics; D015683:Gene Products, gag / Q000096:biosynthesis; D015684:Gene Products, pol / Q000096:biosynthesis; D016679:Genome, Viral; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D019426:Integrases; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014162:Transfection; D014764:Viral Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification
D017931:DNA Primers; D015683:Gene Products, gag; D015684:Gene Products, pol; D014764:Viral Proteins; D004254:DNA Nucleotidyltransferases; D019426:Integrases; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase