Human fibroblasts (KMST-6/RAS cell line) transformed with 60Co gamma-rays and c-Ha-ras oncogene produce a large amount of granulocyte colony-stimulating factor (G-CSF); production is enhanced by cAMP, theophylline, and butyrate.
Human fibroblasts (KMST-6/RAS cell line), which was malignantly transformed in vitro with 60Co gamma-rays and the c-Ha-ras oncogene, produced a large amount of granulocyte colony-stimulating factor (G-CSF). The production was greater during the logarithmic growth phase than during the stationary phase. cAMP and theophylline, alone or in combination, and butyrate significantly enhanced G-CSF production, but dexamethasone or 5-azacytidine did not. Enhanced production of G-CSF by these agents was regulated at the posttranscriptional level. Neither the expression of the ras oncogene nor the tumorigenicity of the cells correlated with the production of G-CSF.
Kawashima
K
K
Department of Surgery, Okayama University Medical School, Japan.
Jahan
I
I
Fushimi
K
K
Mihara
K
K
Namba
M
M
D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002461:Cell Line, Transformed; D003037:Cobalt Radioisotopes; D000242:Cyclic AMP / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism*; D005720:Gamma Rays; D018014:Gene Transfer Techniques; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics; D013806:Theophylline / Q000494:pharmacology*
D002087:Butyrates; D003037:Cobalt Radioisotopes; D020148:Butyric Acid; D016179:Granulocyte Colony-Stimulating Factor; D013806:Theophylline; D000242:Cyclic AMP; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)