title: Changes in beta 1- and beta 2-adrenoceptor mRNA levels in alveolar type II cells during cultivation.
abstract: Alveolar type II cells, the progenitor of type I epithelial cells, synthesize and secrete pulmonary surfactant. In this study, we determined the levels of mRNA for beta 1- and beta 2-adrenoceptor in cultured type II cells using a quantitative RNase protection assay. The mRNA levels for beta 1- and beta 2-adrenoceptors in the freshly isolated cells were 0.51 +/- 0.07 and 1.92 +/- 0.19 amol/microgram total cellular RNA, respectively. In the cells cultured for over 2 days, only beta 1-adrenoceptor mRNA level was remarkably reduced. These results suggest that each mRNA expression may be separately regulated in cultured alveolar type II cells.
authors.affiliation: Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Isohama
authors.forename: T
authors.initials: T
authors.lastname: Matsuo
authors.forename: H
authors.initials: H
authors.lastname: Kai
authors.forename: K
authors.initials: K
authors.lastname: Takahama
authors.forename: T
authors.initials: T
authors.lastname: Miyata
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D008168:Lung / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D018342:Receptors, Adrenergic, beta-1 / Q000096:biosynthesis*; D018343:Receptors, Adrenergic, beta-2 / Q000096:biosynthesis*
chemical_list: D012333:RNA, Messenger; D018342:Receptors, Adrenergic, beta-1; D018343:Receptors, Adrenergic, beta-2