Isolation of immortal human endothelial cells.
To get immortalized endothelial cells, human endothelial cells were transfected with a temperature-sensitive simian virus 40 DNA. The majority of transfected cells showed a prolonged life span and temperature-dependent cell growth. One of the clones, #5-1, grew either at 33 or 37 degrees C and could be cultured more than 110 generations. The cells expressed several endothelial cell specific characteristics.
Kirinaka
H
H
Department of Biotechnology, School of Engineering, Nagoya University, Japan.
Miyake
K
K
Iijima
S
S
D000957:Antigens, Viral, Tumor / Q000235:genetics; D015139:Blotting, Southern; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured / Q000502:physiology*; D003001:Cloning, Molecular; D019040:E-Selectin; D004730:Endothelium, Vascular / Q000166:cytology*; D016679:Genome, Viral; D006133:Growth Substances / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000493:pharmacokinetics; D010957:Plasmids / Q000235:genetics; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics; D013696:Temperature; D010959:Tissue Plasminogen Activator / Q000378:metabolism; D014162:Transfection; D014357:Trypsin / Q000494:pharmacology; D014471:Umbilical Veins / Q000648:ultrastructure; D014841:von Willebrand Factor / Q000276:immunology / Q000378:metabolism
D000957:Antigens, Viral, Tumor; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D006133:Growth Substances; D008077:Lipoproteins, LDL; D017395:Plasminogen Activator Inhibitor 1; D014841:von Willebrand Factor; D006493:Heparin; D014357:Trypsin; D010959:Tissue Plasminogen Activator