title: A chemically defined liquid medium that supports primary isolation of Rochalimaea (Bartonella) henselae from blood and tissue specimens.
abstract: Rochalimaea (Bartonella) henselae is a fastidious, slowly growing, gram-negative bacillus that is an etiologic agent of bacillary angiomatosis, cat scratch disease, and related syndromes. Accumulation of direct microbiologic evidence of the relationship between the organism and the syndromes compatible with cat scratch disease has been hindered by the difficulties in the primary isolation of the organism from infected tissue specimens. A chemically defined liquid medium was developed to support the growth of Rochalimaea species to facilitate study of the organism. This medium was also used successfully to isolate R. henselae from clinical specimens from infected patients and a domestic cat. Recovery of R. henselae in this was more successful than when recovery was attempted on solid agar. This cell-free, extract-free, defined medium additionally supported the growth of Rochalimaea quintana and Afipia felis.
authors.affiliation: Department of Infectious Diseases, Center for AIDS Research, Wilford Hall Medical Center, Lackland Air Force Base, Texas 78236-5300, USA.
authors.forename: M T
authors.initials: MT
authors.lastname: Wong
authors.forename: D C
authors.initials: DC
authors.lastname: Thornton
authors.forename: R C
authors.initials: RC
authors.lastname: Kennedy
authors.forename: M J
authors.initials: MJ
authors.lastname: Dolan
mesh_terms: D016917:Angiomatosis, Bacillary / Q000097:blood / Q000382:microbiology; D000818:Animals; D018416:Bartonella henselae / Q000254:growth & development* / Q000302:isolation & purification; D018413:Bartonella quintana / Q000254:growth & development / Q000302:isolation & purification; D002372:Cat-Scratch Disease / Q000097:blood / Q000382:microbiology; D002415:Cats; D003470:Culture Media; D006090:Gram-Negative Bacteria / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans
chemical_list: D003470:Culture Media