F-actin in mitotic spindles of synchronized suspension culture cells of tobacco visualized by confocal laser scanning microscopy.
TRITC-labelled phalloidin was used to visualize F-actin distribution during mitosis in Nicotiana tabacum BY-2 suspension cells. Aphidicolin was used to synchronize cell suspensions, which enabled sufficient numbers of mitotic cells to be obtained. F-actin was present in the spindle, and its orientation seemed to correlate with the known microtubular arrays. The use of confocal microscopy greatly reduced background fluorescence, and therefore fine actin filaments could be observed in spindles previously thought to be devoid of actin.
Kengen
H M
HM
Department of Experimental Botany, University of Nijmegen, The Netherlands.
Eygensteyn
J
J
van Amstel
T N
TN
D000199:Actins / Q000032:analysis*; D016590:Aphidicolin / Q000494:pharmacology; D002453:Cell Cycle / Q000187:drug effects; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D018613:Microscopy, Confocal / Q000379:methods*; D008870:Microtubules / Q000737:chemistry; D008938:Mitosis; D010590:Phalloidine / Q000031:analogs & derivatives; D010947:Plants, Toxic; D012235:Rhodamines; D008941:Spindle Apparatus / Q000737:chemistry*; D014026:Nicotiana / Q000166:cytology*
D000199:Actins; D005456:Fluorescent Dyes; D012235:Rhodamines; D010590:Phalloidine; D016590:Aphidicolin; C041085:tetramethylrhodaminylphalloidine