Enrichment of peripheral blood stem cells in a primate model following administration of a single dose of rh-IL-1 beta.
In a pre-clinical primate model, the effect of a single intravenous injection of rh-GM-CSF (50 micrograms/kg), rh-IL3 (20 micrograms/kg), rh-IL-1 beta (1 micrograms/kg), rh-G-CSF (50 micrograms/kg) and rh-pIXY 321 (50 micrograms/kg) on peripheral blood mononuclear cell and hematopoietic stem cell concentration was determined. The results indicated that administration of a single dose of rh-IL-1 beta increased the concentrations of hematopoietic stem cells, including CFU-GM progenitors and precursors to CFU-GM. No toxicity was noted with this procedure. None of the remaining cytokines tested caused a significant increase in the peripheral blood hematopoietic stem cell concentration when administered in this manner and only G-CSF caused an increase in peripheral blood mononuclear cell concentrations. Autologous transplantation with limiting numbers of peripheral blood mononuclear cells (1 x 10(7) cells/kg) collected either 24 or 72 h following injection of 1 microgram/kg rh-IL-1 beta conferred a survival advantage to recipients compared with controls. These results imply that a single intravenous administration of rh-IL-1 beta may increase the concentration of peripheral blood stem cells to levels sufficient for transplantation.
Gasparetto
C
C
Allogeneic Bone Marrow Transplantation Service, Memorial Sloan Kettering Cancer Center.
Smith
C
C
Gillio
A
A
Stoppa
A M
AM
Moore
M A
MA
O'Reilly
R J
RJ
D000818:Animals; D001773:Blood Cells / Q000166:cytology / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000008:administration & dosage; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D007375:Interleukin-1 / Q000008:administration & dosage*; D007377:Interleukin-3 / Q000008:administration & dosage; D007958:Leukocyte Count; D008252:Macaca fascicularis; D008297:Male; D008954:Models, Biological; D009504:Neutrophils; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage; D011994:Recombinant Proteins / Q000008:administration & dosage; D014182:Transplantation, Autologous
D007375:Interleukin-1; D007377:Interleukin-3; C078524:PIXY321 fusion protein, recombinant; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor