Recent advances in amelogenin biochemistry.
This paper reviews advances in amelogenin biochemistry in three areas; (i) amelogenin expression; (ii) amelogenin post-translational and post-secretory processing, and (iii) amelogenin structure and function. Recent studies of amelogenin expression have demonstrated that alternative-splicing of mouse amelogenin RNA generates seven distinct mRNAs, coding for amelogenin proteins from 194 to 44 amino acid residues in length. A polyclonal antibody to a sequence of the 194-residue murine amelogenin identified this protein in vivo. While several studies have reported that amelogenins are post-translationally phosphorylated, it has proved difficult to confirm this view. Mass spectrometry studies of bovine and porcine TRAP and LRAP amelogenins have established a phosphoserine residue at position-16 as originally reported by Takagi et al. for a 180-residue bovine amelogenin. Also, we discovered that the detailed mechanism(s) of carboxy-terminal amelogenin proteolytic processing appear different than previously reported. In terms of amelogenin structure, it is well known that amelogenins form aggregated structures. Studies employing a recombinant amelogenin and dynamic light-scattering instrumentation demonstrated aggregate structures of 15-20 nm in radius, corresponding to a mass of 2-3 million daltons. Imaging these aggregates by transmission electron and atomic force microscopy suggested that these structures are equivalent to the "stippled" or "granular" material seen in electron photomicrographs of developing enamel. Collectively, these advances in amelogenin biochemistry lead to a new view of amelogenin structure, processing and functions in enamel biomineralization.
Fincham
A G
AG
Center for Craniofacial Molecular Biology, School of Dentistry, University of Southern California, Los Angeles 90033, USA.
Moradian-Oldak
J
J
D017398:Alternative Splicing / Q000235:genetics; D053523:Amelogenin; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001669:Biochemical Phenomena; D001671:Biochemistry; D002245:Carbon Dioxide / Q000737:chemistry; D002417:Cattle; D003746:Dental Enamel Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D005786:Gene Expression Regulation; D051379:Mice; D010766:Phosphorylation; D010768:Phosphoserine / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000235:genetics; D013329:Structure-Activity Relationship; D013552:Swine
D053523:Amelogenin; C507200:Amelx protein, mouse; D000906:Antibodies; D003746:Dental Enamel Proteins; D012333:RNA, Messenger; D002245:Carbon Dioxide; C041069:carboxyl radical; D010768:Phosphoserine