Development of monoclonal antibodies and capture immunoassays for feline immunodeficiency virus.
We generated monoclonal antibodies (MAB) against feline immunodeficiency virus (FIV) and characterized these MAB by single competition enzyme immunoassays (EIA), immunoblot analysis, and radioimmunoprecipitation. Four MAB identified 3 distinct epitopes of the FIV p24/26 gag major core protein. One MAB recognized the p16/17 gag protein; none recognized envelope proteins. We developed an FIV p26 antigen capture EIA that proved more sensitive (0.5 ng of p26/ml), less expensive, and less time-consuming than reverse transcriptase assay. The same MAB were used to develop an antibody EIA specific for FIV p26. The MAB and capture assays reported should prove useful in FIV diagnosis and research.
Dreitz
M J
MJ
Department of Pathology, Colorado State University, Fort Collins 80523, USA.
Dow
S W
SW
Fiscus
S A
SA
Hoover
E A
EA
D000818:Animals; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D002415:Cats; D002460:Cell Line; D000939:Epitopes / Q000032:analysis; D015683:Gene Products, gag / Q000032:analysis* / Q000276:immunology / Q000302:isolation & purification; D015151:Immunoblotting / Q000379:methods; D016089:Immunodeficiency Virus, Feline / Q000302:isolation & purification*; D007124:Immunoenzyme Techniques; D007668:Kidney; D016719:Radioimmunodetection / Q000379:methods
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D000939:Epitopes; D015683:Gene Products, gag