Inhibition of bovine brain acetylcholinesterase by aluminum.
We report the in vitro inhibitory effect of very low concentrations of aluminum salts (IC50 = 4.1 x 10(-12 M) on bovine brain acetylcholinesterase (AChE). The enzymatic assays were performed using acetylcholine bromide in a buffered pH 7.4 solution at 37 degrees C. The relevant enzyme interacting species is the Al3+ ion, whose concentrations were fixed at pM levels by a citrate metal ion buffer system. The IC50 demonstrates that Al3+ is a potent inhibitor of AChE.
Moraes
M S
MS
Instituto de Química, Universidade Estadual Paulista, Araraquara, SP.
Leite
S R
SR
D000110:Acetylcholinesterase / Q000378:metabolism*; D000535:Aluminum / Q000097:blood / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D002951:Citrates / Q000494:pharmacology; D019343:Citric Acid; D066298:In Vitro Techniques
D002800:Cholinesterase Inhibitors; D002951:Citrates; D019343:Citric Acid; D000535:Aluminum; D000110:Acetylcholinesterase