title: Preparation of sterically stabilized human serum albumin nanospheres using a novel Dextranox-MPEG crosslinking agent.
abstract: Human serum albumin (HSA) nanospheres with a size less than 200 nm in diameter were prepared using a modified coacervation method and crosslinking with methyl polyethylene glycol modified oxidized Dextram (Dextranox-MPEG) which created a sterically stabilizing polyethylene oxide surface layer surrounding the nanospheres. The crosslinking efficiency and the surface characteristics of glutaraldehyde and Dextranox-MPEG crosslinked HSA nanospheres were determined and compared. The zeta potential of the Dextranox-MPEG crosslinked particles was significantly lower than that of glutaraldehyde stabilized particles. The existence of a hydrated steric barrier surrounding the nanospheres was confirmed by an electrolyte and pH induced flocculation test. The Dextranox-MPEG crosslinked nanospheres showed a significantly reduced plasma protein adsorption on the particle surface compared with glutaraldehyde crosslinked nanospheres.
authors.affiliation: Department of Pharmaceutical Sciences, University of Nottingham, University Park, UK.
authors.forename: W
authors.initials: W
authors.lastname: Lin
authors.forename: A G
authors.initials: AG
authors.lastname: Coombes
authors.forename: M C
authors.initials: MC
authors.lastname: Garnett
authors.forename: M C
authors.initials: MC
authors.lastname: Davies
authors.forename: E
authors.initials: E
authors.lastname: Schacht
authors.forename: S S
authors.initials: SS
authors.lastname: Davis
authors.forename: L
authors.initials: L
authors.lastname: Illum
mesh_terms: D002626:Chemistry, Pharmaceutical; D003432:Cross-Linking Reagents; D003911:Dextrans; D006801:Humans; D008863:Microspheres; D010316:Particle Size; D011092:Polyethylene Glycols; D011487:Protein Conformation; D012709:Serum Albumin / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D003432:Cross-Linking Reagents; D003911:Dextrans; D012709:Serum Albumin; C092277:dextranox-methyl polyethylene glycol; D011092:Polyethylene Glycols