title: Substance P increases microvascular permeability via nitric oxide-mediated convective pathways.
abstract: The goal of these studies was to examine the effects of substance P, a tachykinin neuropeptide, on pathways of microvascular permeability. Individual frog mesenteric venular capillaries were cannulated, and albumin apparent permeability coefficients (Ps) were determined by quantitative fluorescence microscopy. Ps of albumin (PsAlb) rose from 6.8 +/- 1.8 (SE) cm.s-1.10(7) at control to 22.3 +/- 2.3 cm.s-1.10(7) when substance P (10(-11) M) was perfused. The effect of increased microvessel permeability induced by substance P (10(-11) M) was blocked with the nonpeptide substance P receptor antagonist CP-96,345 and NG-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthase. PsAlb increased 0.99 cm.s-1.10(7) for every cmH2O increase in microvessel pressure after treatment of the vessel with substance P, demonstrating coupling of albumin flux to transvascular water flow. In conclusion, the mechanism of increased microvessel permeability in response to substance P appears to be the result of receptor-mediated increase in nitric oxide production and formation of water-filled convective pathways presumably located between adjacent endothelial cells.
authors.affiliation: Division of Cardiovascular Medicine, Wadsworth Veterans Affairs Hospital, Los Angeles, California, USA.
authors.forename: L S
authors.initials: LS
authors.lastname: Nguyen
authors.forename: A C
authors.initials: AC
authors.lastname: Villablanca
authors.forename: J C
authors.initials: JC
authors.lastname: Rutledge
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D018481:Convection; D004058:Diffusion; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase; D011894:Rana pipiens; D018040:Receptors, Neurokinin-1 / Q000502:physiology; D012709:Serum Albumin / Q000493:pharmacokinetics; D013373:Substance P / Q000494:pharmacology*
chemical_list: D018040:Receptors, Neurokinin-1; D012709:Serum Albumin; D009569:Nitric Oxide; D013373:Substance P; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases