Purine arabinonucleoside 5'-triphosphates with substituents at 2' position as substrates for DNA polymerases.
Analogues of araNTPs carrying an azido or aminogroup instead of the 2' hydroxyl exhibited substrate properties towards several mammalian and viral DNA polymerases. At the same time, introduction of a bulky hydrophobic DNP group into the 2' position inactivated the compounds as substrates. HSV-1 and CMV DNA polymerases were an interesting exception: they effectively incorporated the modified nucleotide residues with DNP group into the 3'-termini of the DNA chain. This is a reliable distinction of these enzymes from cellular DNA polymerases.
Sharkin
Y A
YA
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Alexandrova
L A
LA
Victorova
L S
LS
Kuznetsova
E V
EV
Krayevsky
A A
AA
D000588:Amines / Q000737:chemistry; D000818:Animals; D001088:Arabinonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001386:Azides / Q000737:chemistry; D001483:Base Sequence; D002417:Cattle; D003587:Cytomegalovirus / Q000201:enzymology; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004253:DNA Nucleotidylexotransferase / Q000737:chemistry / Q000378:metabolism; D004256:DNA Polymerase I / Q000378:metabolism; D017931:DNA Primers / Q000737:chemistry; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004586:Electrophoresis; D018259:Herpesvirus 1, Human / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D010710:Phosphates / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods; D011685:Purine Nucleotides / Q000737:chemistry* / Q000378:metabolism*; D011687:Purines / Q000737:chemistry; D012194:RNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D000588:Amines; D001088:Arabinonucleotides; D001386:Azides; D017931:DNA Primers; D010710:Phosphates; D011685:Purine Nucleotides; D011687:Purines; D004247:DNA; D004253:DNA Nucleotidylexotransferase; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase