title: Mechanism of inhibition of IgE-dependent histamine release from rat mast cells by penasterol and penasterone.
abstract: Penasterol and penasterone, constituents of the Okinawan marine sponge Penares incrustans, dose-dependently inhibited anti-IgE-induced histamine release from rat mast cells. The concentrations of penasterol and penasterone required for 50% inhibition of anti-IgE-induced histamine release (IC50) were 0.5 and 1.5 microM, respectively. Both compounds dose-dependently inhibited phospholipase A2 (PLA2) activity. Moreover, they inhibited anti-IgE-induced [3H]arachidonic acid from rat mast cells. These results suggest that the mechanism of inhibition by these compounds of the histamine release induced by anti-IgE was through the inhibition of PLA2.
authors.affiliation: Forschungsinstitut Borstel, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Takei
authors.forename: A
authors.initials: A
authors.lastname: Umeyama
authors.forename: N
authors.initials: N
authors.lastname: Shoji
authors.forename: S
authors.initials: S
authors.lastname: Arihara
authors.forename: K
authors.initials: K
authors.lastname: Endo
mesh_terms: D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D006636:Histamine Release / Q000187:drug effects* / Q000502:physiology; D007073:Immunoglobulin E / Q000502:physiology*; D066298:In Vitro Techniques; D007810:Lanosterol / Q000031:analogs & derivatives* / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001095:Arachidonic Acids; C093431:penasterone; C093430:penasterol; D007810:Lanosterol; D007073:Immunoglobulin E; D010741:Phospholipases A; D054467:Phospholipases A2