Neurokinin depletion attenuates pulmonary changes induced by antigen challenge in sensitized guinea pigs.
The role of neurokinins in the acute pulmonary response to antigen was studied in guinea pigs that received ovalbumin (50 mg/kg ip) on days 1 and 3 and capsaicin (50 mg/kg sc) on day 21 (OAC); ovalbumin on days 1 and 3 (OA1); capsaicin on day 1 and OA on days 8 and 10 (COA); and ovalbumin on days 8 and 10 (OA2). On day 28, guinea pigs were submitted to ovalbumin aerosol challenge. Maximal values of pulmonary dynamic elastance (Edyn) and pulmonary resistance (RL) were significantly lower in OAC and COA groups compared with OA1 and OA2 groups (P < 0.001). There was no difference between maximal Edyn and RL values obtained in OAC and COA groups. Morphometric analysis of lungs showed significantly (P < 0.05) lower values of contraction index of airways, peribronchial edema, and alveoli over inflation in guinea pigs that received capsaicin compared with intact guinea pigs. Capsaicin treatment did not influence the formation of specific IgG1 anaphylactic antibodies. We conclude that neurokinin depletion results in a decrease in the pulmonary mechanical and inflammatory responses to antigen challenge in sensitized guinea pigs. These effects are observed when capsaicin is given either before or after sensitization.
Warth
M do P
Mdo P
Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, Brazil.
Maldonado
E A
EA
Fernezlian
S M
SM
Leme
A S
AS
Perini
A
A
Saldiva
P H
PH
Martins
M A
MA
D000818:Animals; D000941:Antigens / Q000276:immunology*; D002211:Capsaicin / Q000494:pharmacology; D006168:Guinea Pigs; D007114:Immunization; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008168:Lung / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D008297:Male; D015288:Neurokinin A / Q000037:antagonists & inhibitors / Q000172:deficiency*; D010047:Ovalbumin / Q000276:immunology; D013373:Substance P / Q000037:antagonists & inhibitors
D000941:Antigens; D007074:Immunoglobulin G; D013373:Substance P; D007073:Immunoglobulin E; D015288:Neurokinin A; D010047:Ovalbumin; D002211:Capsaicin