[Inhibitory effect of SP on GABA-activated currents in freshly isolated rat DRG neurons].
The whole-cell patch-clamp technique was used to record GABA-activated currents in freshly isolated DRG neurons. It was found that application of SP (10(-7)-10(-5) mol/L) could induce concentration dependent inward currents in some cells (4/26), while no such current was detectable in the other neurons. However, preapplication of SP could inhibit the inward currents mediated by GABAA receptor (18/22). The inhibitory effect of SP was concentration dependent and reversible, and the desensitization of GABA-activated current could also be enhanced by SP.
Wu
X P
XP
Research Center of Experimental Medicine, Tongji Medical University, Wuhan.
Li
Z W
ZW
Fan
Y Z
YZ
D000818:Animals; D000831:Animals, Newborn; D003864:Depression, Chemical; D004594:Electrophysiology; D005727:Ganglia, Spinal / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013373:Substance P / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D013373:Substance P; D005680:gamma-Aminobutyric Acid