title: [Application of Plasmodium falciparum DNA extract from dried blood spot of falciparum malaria patient in polymerase chain reaction].
abstract: Polymerase chain reaction (PCR) primered with primers 3,4 and 5,6 was performed using DNA extracted from dried blood spot of a falciparum malaria patient from Mengpeng Township, Yunnan using four different methods, including STE-proteinase K-SDS, methanol-proteinase K-SDS, Chelex-100 and Chelex-100-proteinase K to amplify DNA of apical membrane antigen 1 (AMA-1). A 900 bp DNA band could be seen on the ethidium bromide-stained agarose gel electrophoresis of the PCR products when DNA was extracted using all the above-mentioned methods except STE-proteinase K-SDS method. DNA extracted with Chelax-100 was recommended.
authors.affiliation: Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: B
authors.initials: B
authors.lastname: Zhan
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Feng
mesh_terms: D000818:Animals; D001800:Blood Specimen Collection / Q000379:methods; D002675:Child, Preschool; D016054:DNA, Protozoan / Q000302:isolation & purification*; D006801:Humans; D016778:Malaria, Falciparum / Q000235:genetics / Q000469:parasitology*; D008297:Male; D010963:Plasmodium falciparum / Q000235:genetics*; D016133:Polymerase Chain Reaction
chemical_list: D016054:DNA, Protozoan