A high-resolution interval map of the q21 region of the human X chromosome.
In a previous study, we have developed a panel of chromosomal rearrangements for the physical mapping of the q13-q21 region of the human X chromosome (Philippe et al., Genomics 17: 147-152, 1993). Here, we report the physical localization of 36 additional polymorphic markers by polymerase chain reaction analysis. The high density of chromosomal breakpoints in Xq21 allows us to map 58 DNA loci in 22 intervals. As a result, this segment of the X chromosome is saturated with approximately three sequence tagged sites per megabase of DNA, which will facilitate the construction of a YAC contig of this region.
Philippe
C
C
ICRF Laboratories, Wellcome Trust Centre for Human Genetics, Headington, Oxford, United Kingdom.
Arnould
C
C
Sloan
F
F
van Bokhoven
H
H
van der Velde-Visser
S D
SD
Chery
M
M
Ropers
H H
HH
Gilgenkrantz
S
S
Monaco
A P
AP
Cremers
F P
FP
D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D004247:DNA / Q000235:genetics; D005260:Female; D015321:Gene Rearrangement; D005819:Genetic Markers; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D016324:Sequence Tagged Sites; D014960:X Chromosome / Q000235:genetics*
D005819:Genetic Markers; D004247:DNA