Identification of the essential regions for LukS- and H gamma II-specific functions of staphylococcal leukocidin and gamma-hemolysin.
Staphylococcal leukocidin and gamma-hemolysin consist of LukF and LukS for leudocidin and LukF and H gamma II for gamma-hemolysin. To identify the pivotal domain(s) of LukS and H gamma II responsible for their specific cytolytic activities, a series of chimeric genes (lukS/hlg2) were constructed and expressed in Escherichia coli. The results indicate that the C-terminal 24-residue and the N-terminal 57-residue segments of LukS and H gamma II, respectively, are the essential regions for the LukS and H gamma II functions.
Nariya
H
H
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Kamio
Y
Y
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000737:chemistry* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D004926:Escherichia coli; D005677:G(M1) Ganglioside / Q000378:metabolism; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000378:metabolism; D007956:Leukocidins / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013329:Structure-Activity Relationship
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D006460:Hemolysin Proteins; D007956:Leukocidins; D009838:Oligodeoxyribonucleotides; D011993:Recombinant Fusion Proteins; C037956:gamma-hemolysin, Staphylococcus aureus; D005677:G(M1) Ganglioside