Increase in serum NG-hydroxy-L-arginine in rats treated with bacterial lipopolysaccharide.
Aortic rings isolated from rats 4 h after an injection i.p. of 30 mg/kg Escherichia coli lipopolysaccharide showed a marked hyporeactivity to noradrenaline. This effect was paralleled by an increase in the level of nitrite/nitrate in the serum of lipopolysaccharide-treated rats, indicative of an enhanced nitric oxide (NO) synthase activity. Most important, however, the serum concentration of the NO synthase intermediate, NG-hydroxy-L-arginine, was also markedly elevated from 3.7 to 15.8 microM. Circulating NG-hydroxy-L-arginine may thus represent a sensitive and specific marker of NO synthase activity in vivo.
Hecker
M
M
Center of Physiology, J.W. Goethe University Clinic, Frankfurt/M., Germany.
Schott
C
C
Bucher
B
B
Busse
R
R
Stoclet
J C
JC
D000594:Amino Acid Oxidoreductases / Q000097:blood*; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D002956:Citrulline / Q000097:blood; D004926:Escherichia coli / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D009566:Nitrates / Q000097:blood; D019001:Nitric Oxide Synthase; D009573:Nitrites / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism
D008070:Lipopolysaccharides; D009566:Nitrates; D009573:Nitrites; D002956:Citrulline; C068309:N(omega)-hydroxyarginine; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases