title: Enhanced adeno-associated virus vector expression by adenovirus protein-cationic liposome complex. A novel and high efficient way to introduce foreign DNA into endothelial cells.
abstract: Adeno-associated virus vector (AAV), a non-pathogenic integrating vector, can integrate on chromosome 19 in humans. But its gene transfer efficiency is quite low. In this study we combined adenovirus (Adv-5) capsid protein or the Fiber protein of Adv with liposome, termed adenosome (adenovirus protein-cationic liposome complex). This complex can bring AAV/CMV-LacZ to the endothelial cells, and improve the efficiency of gene transfer. It may be a novel, specific, stable and safe gene delivery system and will be widely used in human gene therapy.
authors.affiliation: Cardiovascular Research Institute, Beijing Medical University.
authors.forename: H
authors.initials: H
authors.lastname: Zhou
authors.forename: G
authors.initials: G
authors.lastname: Zeng
authors.forename: X
authors.initials: X
authors.lastname: Zhu
authors.forename: J
authors.initials: J
authors.lastname: Tang
authors.forename: G
authors.initials: G
authors.lastname: Chen
authors.forename: Q
authors.initials: Q
authors.lastname: Huang
authors.forename: T
authors.initials: T
authors.lastname: Peng
authors.forename: B
authors.initials: B
authors.lastname: Hu
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D001483:Base Sequence; D002213:Capsid / Q000235:genetics; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D008081:Liposomes; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D014162:Transfection; D014764:Viral Proteins
chemical_list: D008081:Liposomes; D014764:Viral Proteins