Short report: detection of 72-75-kD and 123-kD fractions of Leishmania antigen in urine of patients with visceral leishmaniasis.
Two polypeptide fractions of 72-75 kD were detected in the urine of 14 of 15 patients with visceral leishmaniasis (VL) and another fraction of 123 kD was found in 10 of the 15 patients by using a Western blot technique. None of these fractions was detected in the urine of 20 controls. These results suggest that antigen detection in urine could be a powerful, noninvasive method for VL diagnosis.
De Colmenares
M
M
Departament de Microbiologia i Parasitologia Sanitaries, Facultat de Farmacia, Universitat de Barcelona, Spain.
Portus
M
M
Riera
C
C
Gallego
M
M
Aisa
M J
MJ
Torras
S
S
Munoz
C
C
D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000652:urine*; D015153:Blotting, Western; D000939:Epitopes / Q000652:urine; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007891:Leishmania / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000652:urine; D008970:Molecular Weight; D010455:Peptides / Q000737:chemistry / Q000652:urine; D011817:Rabbits
D000953:Antigens, Protozoan; D000939:Epitopes; D007106:Immune Sera; D010455:Peptides