Detection of antibodies against phenolic glycolipid-1 (PGL-1), 35-kDa and 30-40-kDa components of Mycobacterium leprae in the cerebrospinal fluid of of leprosy patients.
The involvement of the central nervous system (CNS) in lepromatous leprosy (LL) patients was investigated; 33 patients were examined clinically in detail and upper motor neuron involvement was observed in eight and lower motor neuron in three of these patients. Anti-Mycobacterium leprae antibodies could be detected in the CSF by PGL-1 enzyme-linked immunosorbent assay (ELISA) and monoclonal antibody (MAb) based competitive assays against defined epitopes on the 35-kDa protein and 30-40-kDa polysaccharide (lipoarabinomannan) antigens with MAbs MLO4 and ML34, respectively. Antibodies against PGL-1 and 35-kDa protein were observed in more subjects than antibodies against the 30-40-kDa antigen. Some correlation was observed between the upper motor neuron signs and antibody positivity for 35-kDa and PGL-1 antigens in the CSF of these patients.
Patil
S A
SA
Immunology Laboratory, Central Jalma Institute for Leprosy, Taj Ganj, Agra, India.
Katoch
K
K
Ramu
G
G
Sengupta
U
U
D000328:Adult; D000907:Antibodies, Bacterial / Q000134:cerebrospinal fluid*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001667:Binding, Competitive; D002490:Central Nervous System / Q000503:physiopathology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D006017:Glycolipids / Q000276:immunology; D006801:Humans; D015440:Leprosy, Lepromatous / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged; D016472:Motor Neuron Disease / Q000134:cerebrospinal fluid*; D009166:Mycobacterium leprae / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D006017:Glycolipids; C045169:phenolic glycolipid I, Mycobacterium leprae