title: Zonal distribution of receptor binding of trypsin-activated alpha 2-macroglobulin, alpha 2-macroglobulin receptor-associated protein, lactoferrin and transferrin on rat liver parenchymal cells.
abstract: Periportal and perivenous rat liver parenchymal cells were isolated according to the digitonin-collagenase perfusion method. Affinities and maximal specific binding of a conjugate of glutathione S-transferase and the alpha 2-macroglobulin receptor-associated protein (GST-39kDaP), of lactoferrin and of transferrin to freshly isolated periportal parenchymal cells in vitro were not significantly different from values obtained with perivenous cells. It is concluded that the receptors for these three ligands show a zonally homogeneous expression in rat liver. The zonal homogeneity in binding observed for GST-39kDaP is at variance with the 1.5-fold higher periportal over perivenous binding of trypsin-activated alpha 2-macroglobulin. Since GST-39kDaP as well as trypsin-activated alpha 2-macroglobulin are ligands for the alpha 2-macroglobulin receptor/low-density lipoprotein receptor-related protein, it is suggested that GST-39kDaP can bind to (an) additional receptor(s) with a higher perivenous expression. The zonal homogeneity observed with lactoferrin, an inhibitor of ligand binding to the lipoprotein remnant receptor, may indicate zonal homogeneity of the lipoprotein remnant receptor. The observed zonal homogeneity of the transferrin receptor suggests an equal and essential need for iron by parenchymal cells across the rat liver acinus in vivo.
authors.affiliation: Division of Biopharmaceutics, Leiden/Amsterdam Center for Drug Research, Sylvius Laboratories, The Netherlands.
authors.forename: A H
authors.initials: AH
authors.lastname: Voorschuur
authors.forename: J
authors.initials: J
authors.lastname: Kuiper
authors.forename: W L
authors.initials: WL
authors.lastname: Van Noort
authors.forename: T J
authors.initials: TJ
authors.lastname: Van Berkel
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002469:Cell Separation; D005260:Female; D005982:Glutathione Transferase; D006023:Glycoproteins / Q000378:metabolism*; D026563:LDL-Receptor Related Protein-Associated Protein; D007781:Lactoferrin / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D008565:Membrane Proteins / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis / Q000378:metabolism; D011971:Receptors, Immunologic / Q000032:analysis / Q000378:metabolism; D011990:Receptors, Transferrin / Q000032:analysis / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D006023:Glycoproteins; D026563:LDL-Receptor Related Protein-Associated Protein; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D008565:Membrane Proteins; D018832:Molecular Chaperones; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D011990:Receptors, Transferrin; D000511:alpha-Macroglobulins; C038340:lactoferrin receptors; D005982:Glutathione Transferase; D007781:Lactoferrin; D014357:Trypsin