title: Expression and distribution of functional integrins in rat CNS glia.
abstract: In previous studies, we have reported on the expression of beta 1 integrins in type 1 astrocytes and their function in cell-substratum attachment (Tawil et al., J Cell Biol 120:261-271, 1993). Here we extend those findings by providing evidence that type 1 astrocytes express integrins of the beta 3 and possibly beta 4 subclasses and that the former (alpha v beta 3) functions in attachment by recognizing the peptide, Arg-Gly-Asp, in vitronectin. In addition, we have examined immunocytochemically the expression of beta 1 integrins on type 2 astrocytes and oligodendrocytes. The pattern of expression of integrins on these two cell types is distinct from type 1 astrocytes; most notably type 1 astrocytes but not oligodendrocytes or type 2 astrocytes express alpha 1 beta 1 heterodimers. Since type 2 astrocytes and oligodendrocytes originate from a common precursor (O-2A), the alpha 1 beta 1 heterodimer may be a functional marker which distinguishes O-2A-derived cells from those of the type 1 astrocyte lineage.
authors.affiliation: Center for Neuroscience Research, McGill University, Montreal General Hospital Research Institute, Quebec, Canada.
authors.forename: N J
authors.initials: NJ
authors.lastname: Tawil
authors.forename: P
authors.initials: P
authors.lastname: Wilson
authors.forename: S
authors.initials: S
authors.lastname: Carbonetto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002478:Cells, Cultured; D002525:Cerebellar Cortex / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D003094:Collagen; D005353:Fibronectins; D006023:Glycoproteins; D007150:Immunohistochemistry; D039222:Integrin alpha1beta1; D019012:Integrin beta1; D016023:Integrins / Q000032:analysis / Q000096:biosynthesis* / Q000502:physiology; D007797:Laminin; D008969:Molecular Sequence Data; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017469:Receptors, Fibronectin; D019096:Vitronectin
chemical_list: D000911:Antibodies, Monoclonal; D005353:Fibronectins; D006023:Glycoproteins; D039222:Integrin alpha1beta1; D019012:Integrin beta1; D016023:Integrins; D007797:Laminin; D017469:Receptors, Fibronectin; D019096:Vitronectin; D003094:Collagen