Rapid HPLC assay with coulometric detection for norepinephrine and 3-methoxy-4-hydroxyphenylglycol in the mouse brain.
For the rapid assay of norepinephrine (NE) and its major metabolite, 3-methoxy-4-hydroxyphenylglycol (MHPG) in the mouse brain, we developed a simple method using isocratic HPLC with coulometric detection. This method permits NE and MHPG assay within 5 min in one chromatographic run. Within-run coefficients of variation for NE and MHPG in the working standard solution were 0.8% and 0.6% (n = 50), respectively. The detector responses were linear from 0.025 to 100 pmol for NE and from 0.05 to 100 pmol for MHPG in the working standard solution. Using this method, the NE and MHPG concentrations were measured in discrete brain areas of the mouse prior treatment with or without alpha-methyl-p-tyrosine or N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP4).
Murai
S
S
Department of Pharmacology, School of Dentistry, Iwate Medical University, Morioka, Japan.
Saito
H
H
Masuda
Y
Y
Ohkubo
N
N
Itoh
T
T
D018663:Adrenergic Agents / Q000494:pharmacology; D000818:Animals; D001596:Benzylamines / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004563:Electrochemistry; D004566:Electrodes; D005625:Frontal Lobe / Q000187:drug effects / Q000378:metabolism*; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008297:Male; D008734:Methoxyhydroxyphenylglycol / Q000032:analysis / Q000378:metabolism*; D008781:Methyltyrosines / Q000494:pharmacology; D051379:Mice; D009638:Norepinephrine / Q000032:analysis / Q000378:metabolism*; D012015:Reference Standards; D014446:Tyrosine 3-Monooxygenase / Q000037:antagonists & inhibitors; D019805:alpha-Methyltyrosine
D018663:Adrenergic Agents; D001596:Benzylamines; D008781:Methyltyrosines; D008734:Methoxyhydroxyphenylglycol; D019805:alpha-Methyltyrosine; D014446:Tyrosine 3-Monooxygenase; C012102:DSP 4; D009638:Norepinephrine