Activity of the yeast FLP recombinase in Arabidopsis.
The coding sequence for FLP recombinase, originally from the 2 mu plasmid of Saccharomyces cerevisiae, was introduced into Arabidopsis behind the cauliflower mosaic virus 35S promoter. FLP activity was monitored by the glucuronidase activity resulting from inversion of an antisense-oriented GUS reporter gene flanked by a pair of FRT target sites in inverted repeat. FLP-dependent Gus activity was observed in both transient assays and transgenic plants. The FLP system will be useful for a variety of in planta genetic manipulations.
Sonti
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RV
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139-4307, USA.
Tissier
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AF
Wong
D
D
Viret
J F
JF
Signer
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ER
D017360:Arabidopsis / Q000378:metabolism*; D007446:Chromosome Inversion; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D005656:Fungal Proteins; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase