title: Evaluation of pig lungs following an experimental challenge with Actinobacillus pleuropneumoniae serotype 1 and 5 in pigs inoculated with either hemolysin protein and/or outer membrane proteins.
abstract: Histopathological changes were compared in pigs challenged with Actinobacillus pleuropneumoniae serotype 1 and serotype 5 after inoculation with subunit vaccines. The vaccines consisted of outer membrane protein and/or hemolysin protein isolated from Actinobacillus pleuropneumoniae serotype 1 or both subunits combined. Twenty-seven cross-bred pigs were separated into six groups: Groups I and IV were vaccinated and boostered with 1500 micrograms outer membrane protein; Groups II and V were vaccinated and boostered with 250 micrograms hemolysin protein; Groups III and VI were vaccinated and boostered with a combination of 1500 micrograms outer membrane protein and 250 micrograms hemolysin protein. Groups I, II and III were challenged with A. pleuropneumoniae serotype 1; and Groups IV, V and VI were challenged with A. pleuropneumoniae serotype 5. Groups III and VI demonstrated the least severe lung tissue damage, with significantly lower (P < 0.05) lung involvement as compared to the other groups. Lesions were noted in all six groups. These results showed that complete protection against A. pleuropneumoniae infection was not feasible using a subunit vaccine consisting of just outer membrane protein and hemolysin protein, and that some cross-protection did occur.
authors.affiliation: Department of Animal Science, College of Biology and Agriculture, Brigham Young University, Provo, UT 84602, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Madsen
authors.forename: K G
authors.initials: KG
authors.lastname: Carnahan
authors.forename: R N
authors.initials: RN
authors.lastname: Thwaits
mesh_terms: D000189:Actinobacillus Infections / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000276:immunology*; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology / Q000302:isolation & purification; D001428:Bacterial Vaccines; D006460:Hemolysin Proteins / Q000276:immunology / Q000302:isolation & purification; D008168:Lung / Q000473:pathology; D011001:Pleuropneumonia / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D013552:Swine; D013553:Swine Diseases / Q000473:pathology / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D006460:Hemolysin Proteins