title: Immunocytochemical study of NA+ K(+)-ATPase alpha 1 and beta 1 subunits in human and rat normal hepatocytes using confocal microscopy.
abstract: Hepatic Na+ K(+)-ATPase was recently shown to be composed of two alpha 1 and beta 1 subunits similar to that of kidney. Its localization on hepatocyte plasma membranes was not clearly established. We have studied the localization of alpha 1 and beta 1 subunits using immunocytochemical method and confocal microscopy. In accordance with previous cytochemical findings, the catalytic alpha 1 subunit was distributed in basolateral and bile canalicular membranes of hepatocytes. The beta 1 subunit was not demonstrated, due to its very low amount in the liver. The controversy about the bile canalicular localization was discussed.
authors.affiliation: Laboratoire de Pathologie Cellulaire et Métabolique du Foie, Faculté de Médecine, Marseille, France.
authors.forename: L
authors.initials: L
authors.lastname: Benkoël
authors.forename: A M
authors.initials: AM
authors.lastname: Benoliel
authors.forename: J
authors.initials: J
authors.lastname: Brisse
authors.forename: B
authors.initials: B
authors.lastname: Sastre
authors.forename: P
authors.initials: P
authors.lastname: Bongrand
authors.forename: A
authors.initials: A
authors.lastname: Chamlian
mesh_terms: D000818:Animals; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000201:enzymology*; D008297:Male; D018613:Microscopy, Confocal; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis* / Q000737:chemistry
chemical_list: D000254:Sodium-Potassium-Exchanging ATPase