Quantitative computerized analysis of silver-stained and Coomassie Blue-stained two-dimensional protein maps.
Using the two-dimensional gel electrophoresis and two systems for computer analysis of the resulting maps, we obtained a good quantitative reproducibility of individual spots of silver-stained and Coomassie Blue-stained gels from mouse fetus liver samples. The GelScan-XL system was established for the evaluation of silver-stained gels. The Gel-Image software has shown to be capable of the analysis of Coomassie Blue-stained gels and autoradiographs. For these systems the linear relationship between defined concentrations of protein loaded on the gel and the resulting integrated intensities of spots were examined. The results show that both systems are efficient to detect quantitative changes in protein expression correlated with malformations in mouse fetuses.
Hillebrandt
S
S
Institut für Medizinische Strahlenbiologie, Universitätsklinik Essen, Bundesrepublik Deutschland.
Streffer
C
C
D000818:Animals; D003201:Computers; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005333:Fetus; D007202:Indicators and Reagents; D008099:Liver / Q000737:chemistry*; D051379:Mice; D008815:Mice, Inbred Strains; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012394:Rosaniline Dyes; D012834:Silver; D012867:Skin / Q000737:chemistry*; D012984:Software; D013194:Staining and Labeling
D007202:Indicators and Reagents; D011506:Proteins; D012394:Rosaniline Dyes; D012834:Silver; C048139:Coomassie blue