Competitive binding analysis of monoclonal antibodies reactive with porcine alveolar macrophages using anti-CD14 and anti-CD18.
Four monoclonal antibodies (mAb) from the myeloid subset panel of the First International Swine CD Workshop (74-22-15, DH59B, PM16-6, and MUC21A) were analyzed using competitive inhibition studies with anti-human CD14 (My4) and anti-human/anti-porcine CD18 (MHM23) on porcine alveolar macrophages. Results suggested that none of the mAb tested recognized CD14 or CD18 on porcine alveolar macrophages. Additionally, the cross-reactivity of My4 with porcine alveolar macrophages was established.
Kielian
T
T
Department of Anatomy and Physiology, Kansas State University, Manhattan 66506.
McVey
D S
DS
Davis
W C
WC
Kim
Y B
YB
Blecha
F
F
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D015703:Antigens, CD / Q000276:immunology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology*; D001667:Binding, Competitive / Q000276:immunology*; D018821:CD18 Antigens / Q000276:immunology*; D002478:Cells, Cultured; D003429:Cross Reactions / Q000276:immunology; D006801:Humans; D018950:Lipopolysaccharide Receptors; D016676:Macrophages, Alveolar / Q000276:immunology*; D013552:Swine / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D018821:CD18 Antigens; D018950:Lipopolysaccharide Receptors