title: Intracellular immunization of human fetal cord blood stem/progenitor cells with a ribozyme against human immunodeficiency virus type 1.
abstract: Successful treatment of human immunodeficiency virus infection may ultimately require targeting of hematopoietic stem cells. Here we used retroviral vectors carrying the ribozyme gene to transduce CD34+ cells from human fetal cord blood. Transduction and ribozyme expression had no apparent adverse effect on cell differentiation and/or proliferation. The macrophage-like cells, differentiated from the stem/progenitor cells in vitro, expressed the ribozyme gene and resisted infection by a macrophage tropic human immunodeficiency virus type 1. These results suggest the feasibility of stem cell gene therapy for human immunodeficiency virus-infected patients.
authors.affiliation: Department of Medicine, University of California, San Diego, La Jolla 92093-0665.
authors.forename: M
authors.initials: M
authors.lastname: Yu
authors.forename: M C
authors.initials: MC
authors.lastname: Leavitt
authors.forename: M
authors.initials: M
authors.lastname: Maruyama
authors.forename: O
authors.initials: O
authors.lastname: Yamada
authors.forename: D
authors.initials: D
authors.lastname: Young
authors.forename: A D
authors.initials: AD
authors.lastname: Ho
authors.forename: F
authors.initials: F
authors.lastname: Wong-Staal
mesh_terms: D015703:Antigens, CD / Q000032:analysis*; D018952:Antigens, CD34; D001483:Base Sequence; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004258:DNA Polymerase III / Q000235:genetics; D005312:Fetal Blood / Q000166:cytology; D005822:Genetic Vectors; D015497:HIV-1 / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000276:immunology; D006801:Humans; D018189:Immunomagnetic Separation; D008264:Macrophages / Q000821:virology*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D016337:RNA, Catalytic / Q000096:biosynthesis / Q000235:genetics*; D012190:Retroviridae; D014170:Transformation, Genetic; D014779:Virus Replication
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D016337:RNA, Catalytic; D004258:DNA Polymerase III