The large subunit of HIV-1 reverse transcriptase interacts with beta-actin.
HIV-1 reverse transcriptase is a dimeric enzyme mainly involved in the replication of the viral genome. A filamentous phage cDNA expression library from human lymphocytes was used to select cellular proteins interacting with HIV-1 reverse transcriptase Affinity selections using the bacterially expressed monomeric large subunit of reverse transcriptase (p66) yielded host beta-actin. This clone was expressed as glutathione-S-transferase fusion protein which was identified by using a specific antibody against beta-actin. Furthermore we show that also the eukaryotic beta-actin binds to either the large subunit of reverse transcriptase or to the Pol precursor polyprotein in vitro. The reverse transcriptase/beta-actin interaction might be important for the secretion of HIV-1 virions.
Hottiger
M
M
Department of Veterinary Biochemistry, University of Zürich-Irchel, Switzerland.
Gramatikoff
K
K
Georgiev
O
O
Chaponnier
C
C
Schaffner
W
W
Hübscher
U
U
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D017931:DNA Primers; D018076:DNA, Complementary; D015683:Gene Products, gag / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000201:enzymology*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D054301:gag Gene Products, Human Immunodeficiency Virus; D054302:pol Gene Products, Human Immunodeficiency Virus
D000199:Actins; D017931:DNA Primers; D018076:DNA, Complementary; D015683:Gene Products, gag; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; D054302:pol Gene Products, Human Immunodeficiency Virus; C091950:pr160 gag-pol precursor protein, Human immunodeficiency virus 1; D005982:Glutathione Transferase; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase