Mapping of cross-reacting and serotype-specific epitopes on the VP3 structural protein of the infectious bursal disease virus (IBDV).
The binding sites of a panel of monoclonal antibodies cross-reacting with the structural protein VP3 of the two serotypes of the infectious bursal disease virus (IBDV) could be mapped to four segments of the VP3 gene. Two of these antigenic domains also carry epitopes which are specific for one serotype only. Formation of the common or type-specific epitopes is in agreement with homologous or mismatching amino acid sequences yielding hydrophilic segments on the VP3 polypeptide. These antigenic patterns obtained by immunoblotting could be verified by a competitive ELISA.
Mahardika
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GN
Institut für Virologie, Justus-Liebig-Universität Giessen, Federal Republic of Germany.
Becht
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H
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D002642:Chick Embryo; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D007243:Infectious bursal disease virus / Q000276:immunology*; D012703:Serotyping; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology*
D000939:Epitopes; D015678:Viral Structural Proteins