Characterization of rainbow trout C-polysaccharide binding proteins.
Two proteins were isolated from rainbow trout sera by affinity chromatography using C-polysaccharide-Sepharose 4B column. One was appropriate to the trout C-reactive protein (CRP) that was reported previously (Murai et al. 1990. Dev. Comp. Immunol. 14: 49-58). The other was a newly found protein that had apparent molecular weight of 135,000 on native gradient polyacrylamide gel electrophoresis, and isoelectric points of 5.2-5.8. The N-terminal sequence (twenty amino acids) of the newly found protein was similar to CRPs from other species (e.g. 44% homology with plaice CRP). On electron microscope, the newly found protein was observed as pentagonal symmetry structure.
Murata
M
M
Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Kodama
H
H
Onuma
M
M
D000595:Amino Acid Sequence; D000818:Animals; D002097:C-Reactive Protein / Q000737:chemistry* / Q000302:isolation & purification; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002846:Chromatography, Affinity; D017124:Conserved Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008322:Mammals / Q000097:blood; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017686:Oncorhynchus mykiss / Q000097:blood*; D011134:Polysaccharides; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D011134:Polysaccharides; D002097:C-Reactive Protein