Pax3: a paired domain gene as a regulator in PNS myelination.
Pax3 RNA is expressed in neural crest when Schwann cell (SC) precursors migrate to the PNS. Pax3 RNA and SC markers were monitored in sciatic nerves of mice during development and nerve repair. An inverse correlation was observed between expression of Pax3 RNA and myelin basic protein (MBP). Inverse correlation was also observed in SC primary cultures. Treating cultures with forskolin, an adenylate cyclase agonist, repressed Pax3 RNA, GFAP, NGFR, N-CAM, and L1 and elevated MBP. Subsequent microinjection with Pax3 expression vector elevated Pax3 RNA, GFAP, NGFR, N-CAM, and L1 and repressed MBP. Thus, Pax3 is likely involved in the differentiation pathway to myelinating SCs. Pax3 repressed a 1.3 kb MBP promoter fragment in cotransfection assays, suggesting that it represses MBP transcription.
Kioussi
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Abteilung für Molekulare Zellbiologie, Max-Planck Institut für Biophysikalische Chemie, Göttingen, Federal Republic of Germany.
Gross
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MK
Gruss
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D000818:Animals; D001369:Axons / Q000502:physiology; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000494:pharmacology; D005786:Gene Expression Regulation; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D051379:Mice; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism; D009186:Myelin Sheath / Q000502:physiology*; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D017933:Peripheral Nervous System / Q000502:physiology*; D011401:Promoter Regions, Genetic; D012313:RNA / Q000032:analysis / Q000378:metabolism; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism; D012583:Schwann Cells / Q000378:metabolism; D012584:Sciatic Nerve / Q000378:metabolism; D014157:Transcription Factors
D015816:Cell Adhesion Molecules, Neuronal; D004268:DNA-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D004676:Myelin Basic Protein; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D017475:Receptors, Nerve Growth Factor; D014157:Transcription Factors; C068686:Pax3 protein, mouse; D005576:Colforsin; D012313:RNA