[Alleles in chromosome 10p21-26 in malignant gliomas].
Loss of genetic material on chromosome 10 is regarded as a prominent feature in the genesis of glioblastomas. To use chromosome 10 deletions as diagnostic markers for glioblastomas we investigated, if the loss of chromosome 10 material could be restricted on the region 10q21-26. By PCR microsatellite analysis on frozen tissue and paraffin material from the ZULCH brain tumor collection we found (1) loss of heterozygosity in 10q21-26 in 75% of the investigated DNA from frozen tissue and (2) an interstitial loss in the region of the microsatellite marker D10S186. The combined immunohistochemical analysis of overexpression of EGFR, EGF and TGF alpha with LOH on chromosome 10 showed that chromosome 10 deletions are not exclusively bound to EGFR overexpression.
Oberstrass
J
J
Institut für Neuropathologie, Heinrich-Heine-Universität, Düsseldorf.
Ring
G U
GU
Vogeley
K T
KT
Kramm
C M
CM
Cobbers
J M
JM
Wirths
J
J
Bilzer
T
T
Wechsler
W
W
D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D004273:DNA, Neoplasm / Q000032:analysis; D004276:DNA, Satellite / Q000032:analysis; D004815:Epidermal Growth Factor / Q000096:biosynthesis; D066246:ErbB Receptors / Q000096:biosynthesis; D015870:Gene Expression; D005909:Glioblastoma / Q000235:genetics* / Q000473:pathology; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D010232:Paraffin; D016133:Polymerase Chain Reaction / Q000379:methods; D016211:Transforming Growth Factor alpha / Q000096:biosynthesis
D004273:DNA, Neoplasm; D004276:DNA, Satellite; D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor; D010232:Paraffin; D066246:ErbB Receptors