Dextran 70 administration after trauma-hemorrhagic shock does not impair cellular immune functions.
OBJECTIVE
Although the effects of the colloid dextran 70 on induction of anaphylactoid reactions or reticuloendothelial phagocytosis have been examined previously, its effects on specific cell-mediated immunity after trauma-hemorrhage shock remain unknown.


METHODS
Nonheparinized C3H/HeN mice underwent a laparotomy, were bled, and then maintained at a blood pressure of 35 mm Hg for 60 minutes. Then they were resuscitated with either 4 x the shed blood volume as lactated Ringer's solution (LRS) or 2 x LRS + 1 x dextran 70. Control mice underwent all operative protocols but were neither hemorrhaged, nor resuscitated. At 2 or 24 hours posthemorrhage, serum, splenocytes (SPL), and peritoneal macrophages (pM phi, splenic Mo (sM phi) were obtained. Bioassays were used to determine interleukin-2 (IL-2), IL-3, IL-6, and SPL proliferation.


RESULTS
Trauma-hemorrhage markedly depressed lymphokine release, splenocyte proliferation, and IL-6 release at 2 hours after the insult. The combination of LRS + dextran did not restore lymphocyte functions, but also did not further suppress them. The release of IL-6 by pM phi and sM phi at 2 and 24 hours after dextran infusion and serum IL-6 remained at the same level as in LRS-treated animals.


CONCLUSIONS
The combination of LRS and colloid dextran 70 does not adversely affect ex vivo cell-mediated immune functions during the first 24 hours after its administration after trauma-hemorrhage. Thus, from the immunological standpoint, dextran is a safe resuscitation adjunct.
Schmand
J F
JF
Department of Surgery, Michigan State University, East Lansing 48824-1315.
Ayala
A
A
Morrison
M H
MH
Chaudry
I H
IH
D000704:Analysis of Variance; D000818:Animals; D002478:Cells, Cultured; D003911:Dextrans / Q000009:adverse effects* / Q000276:immunology; D005440:Fluid Therapy; D007111:Immunity, Cellular; D007376:Interleukin-2 / Q000096:biosynthesis / Q000097:blood; D007377:Interleukin-3 / Q000096:biosynthesis / Q000097:blood; D015850:Interleukin-6 / Q000096:biosynthesis / Q000097:blood; D008264:Macrophages / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D010537:Peritoneum / Q000166:cytology; D011897:Random Allocation; D012771:Shock, Hemorrhagic / Q000188:drug therapy / Q000276:immunology*; D012774:Shock, Traumatic / Q000188:drug therapy / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism
D003911:Dextrans; D007376:Interleukin-2; D007377:Interleukin-3; D015850:Interleukin-6