Ex vivo expansion of hematopoietic progenitor cells in human cord blood: an effect enhanced by cord blood serum.
The concept of ex vivo expansion of the human stem/progenitor cell population was tested in this study. Cord blood mononuclear cells were isolated and cultured in the presence of SCF, IL-3, and either cord blood serum (CBS) or fetal calf serum (FCS). After 8 days of incubation with CBS or FCS, the number of CFU-GM was increased ninefold and four-and-a-half-fold, respectively. However, the enhancing effect lasted for only a short period. This study demonstrates that CBS could contain growth factors expanding progenitor cells. Whether the ability of CBS to enhance the CFU-GM expanding capacity is attributable to a novel factor or factors, or represents effects of other known cytokines, remains to be determined.
Kurata
H
H
Department of Obstetrics and Gynecology, Niigata University School of Medicine, Japan.
Takakuwa
K
K
Tanaka
K
K
D002454:Cell Differentiation; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D007377:Interleukin-3 / Q000502:physiology; D019089:Stem Cell Factor / Q000502:physiology
D007377:Interleukin-3; D019089:Stem Cell Factor