title: Specific cleavage of two target sequences of the tobacco mosaic virus RNA by a di-component ribozyme.
abstract: Two di-component ribozymes RZ34 and RZ13, the simplest forms of multi-component ribozymes, by ligating mono-ribozymes RZ3 and RZ4 and RZ1 and RZ3 were constructed, respectively. The in vitro results indicate that each di-component ribozyme is able to cleave two different TMV transcripts, with the specificity and efficiency similar to their mono-ribozyme counterparts. Possible effects on the specificity and efficiency by temperature and the positions of the component-ribozymes in these di-component ribozymes were also studied.
authors.affiliation: National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, Chinese Academy of Sciences.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Jin
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: R
authors.initials: R
authors.lastname: Song
authors.forename: Z
authors.initials: Z
authors.lastname: Xu
mesh_terms: D001483:Base Sequence; D008969:Molecular Sequence Data; D016337:RNA, Catalytic / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D013379:Substrate Specificity; D013696:Temperature; D014027:Tobacco Mosaic Virus / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D016337:RNA, Catalytic; D012367:RNA, Viral