Antilisterial activity of thermophilin 347, a bacteriocin produced by Streptococcus thermophilus.
Streptococcus thermophilus 347 isolated from yogurt produces a bacteriocin referred as thermophilin 347. The bacteriocin was evidenced in the neutralized, filtered and catalase treated culture supernatant fluid of the producer strain. After partial purification, thermophilin 347 exhibited a bactericidal effect against Listeria monocytogenes and several closely related lactic acid bacteria. The activity of thermophilin 347 was lost after protease treatment but was maintained after heating at 100 degrees C for 1 h; after autoclaving at 121 degrees C for 15 min the activity was reduced by 50%. SDS-PAGE of partially purified thermophilin 347 was used to detect bacteriocin activity corresponding to an apparent molecular mass between 2.5 and 6.2 KDa.
Villani
F
F
Istituto di Microbiologia Agraria e Stazione di Microbiologia Industriale, Universita degli Studi di Napoli Federico II, Portici, Italy.
Pepe
O
O
Mauriello
G
G
Salzano
G
G
Moschetti
G
G
Coppola
S
S
D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D008970:Molecular Weight; D013291:Streptococcus / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D001430:Bacteriocins