title: A novel class of embryonic cell adhesion glycan epitopes is expressed in human colon carcinomas.
abstract: A new class of acidic glycans isolated from marine sponges and sea urchin embryos was shown to mediate cell adhesion via carbohydrate-carbohydrate interactions. Cell aggregation blocking monoclonal antibodies (Block 1 and Block 2 mAbs) directed against these polysaccharides localized functional epitopes in embryonic sea urchin gut. Immunofluorescence light microscopy of human colon carcinomas and healthy colon samples with Block 1 and Block 2 mAbs established that the carbohydrate structures similar to the invertebrate acidic glycan adhesion molecules are also expressed in humans. The Block 1 mAb labeled basal and apical lamina of tumor cells, whereas the Block 2 bound exclusively to the apical part of the epithelium. In normal tissue whole goblet cell membrane was stained with both antibodies indicating that transformation leads to spatial rearrangement of glycan antigens. Acidic glycans from human colon carcinomas and normal colon were isolated after delipidation, and complete pronase and DNase digestion, by gel filtration and adsorption to anion exchange membranes. Immunodot assay with Block 1 and Block 2 mAbs revealed that tumor cells have elevated expression of both carbohydrate structures. These results suggest that the acidic glycan adhesion molecules, originally found in sponges and sea urchin embryos, may represent a new class of carbohydrate carcino-embryonal antigens involved in cellular interactions associated with morphogenesis, metastasis and renewal of adult tissue.
authors.affiliation: Department of Research, University Hospital of Basel, Switzerland.
authors.forename: G N
authors.initials: GN
authors.lastname: Misevic
authors.forename: O
authors.initials: O
authors.lastname: Popescu
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D003106:Colon / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D004625:Embryo, Nonmammalian / Q000502:physiology*; D000939:Epitopes / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D011134:Polysaccharides / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D011161:Porifera / Q000196:embryology; D012016:Reference Values; D012617:Sea Urchins / Q000196:embryology
chemical_list: D015815:Cell Adhesion Molecules; D000939:Epitopes; D011134:Polysaccharides