title: Loss of chromosome 17 loci in prostate cancer detected by polymerase chain reaction quantitation of allelic markers.
abstract: Using a polymerase chain reaction/microsatellite marker system, we demonstrated that 6 of 22 (27%) clinical stage B (early) primary prostate tumors showed loss of heterozygosity at one or more of five loci on chromosome 17. The sensitivity of this study was increased by use of a PhosphorImager and statistical analysis of replicate tumor-normal DNA pairs. Two patients showed tumor-specific interstitial loss at a locus in close proximity to the familial breast cancer gene BRCA1. These findings suggest that genes on the proximal long arm of chromosome 17 play a pivotal role in the early development of at least a subset of prostatic tumors.
authors.affiliation: Department of Human Genetics, University of Utah School of Medicine, Salt Lake City 84132, USA.
authors.forename: A R
authors.initials: AR
authors.lastname: Brothman
authors.forename: M R
authors.initials: MR
authors.lastname: Steele
authors.forename: B J
authors.initials: BJ
authors.lastname: Williams
authors.forename: E
authors.initials: E
authors.lastname: Jones
authors.forename: S
authors.initials: S
authors.lastname: Odelberg
authors.forename: H M
authors.initials: HM
authors.lastname: Albertsen
authors.forename: L B
authors.initials: LB
authors.lastname: Jorde
authors.forename: L R
authors.initials: LR
authors.lastname: Rohr
authors.forename: R A
authors.initials: RA
authors.lastname: Stephenson
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000235:genetics*
chemical_list: D005819:Genetic Markers