Changes in beta 1- and beta 2-adrenoceptor mRNA levels in alveolar type II cells during cultivation.
Alveolar type II cells, the progenitor of type I epithelial cells, synthesize and secrete pulmonary surfactant. In this study, we determined the levels of mRNA for beta 1- and beta 2-adrenoceptor in cultured type II cells using a quantitative RNase protection assay. The mRNA levels for beta 1- and beta 2-adrenoceptors in the freshly isolated cells were 0.51 +/- 0.07 and 1.92 +/- 0.19 amol/microgram total cellular RNA, respectively. In the cells cultured for over 2 days, only beta 1-adrenoceptor mRNA level was remarkably reduced. These results suggest that each mRNA expression may be separately regulated in cultured alveolar type II cells.
Isohama
Y
Y
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
Matsuo
T
T
Kai
H
H
Takahama
K
K
Miyata
T
T
D000818:Animals; D002478:Cells, Cultured; D008168:Lung / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D018342:Receptors, Adrenergic, beta-1 / Q000096:biosynthesis*; D018343:Receptors, Adrenergic, beta-2 / Q000096:biosynthesis*
D012333:RNA, Messenger; D018342:Receptors, Adrenergic, beta-1; D018343:Receptors, Adrenergic, beta-2