title: Colocalization of NADPH-diaphorase with neuropeptides in the intrapancreatic neurons of the chicken.
abstract: Colocalization of nitric oxide with neuropeptides was investigated in the chicken pancreas by use of double staining combined with the indirect immunofluorescence technique and histochemistry for NADPH-diaphorase, a specific marker for neural nitric oxide synthase. NADPH-diaphorase positive ganglia were easily detected in the interlobular connective tissue. Many NADPH-diaphorase positive ganglion cells also showed immunoreactivity for VIP (80.9%) or galanin (76.2%). Some ganglion cells showed enzyme activity only (about 20%). Very few neurons were NADPH-diaphorase negative, but immunopositive for VIP (2.0%) or galanin (3.7%). The present study provides evidence that nitric oxide colocates with VIP and galanin in the chicken pancreas.
authors.affiliation: Laboratory of Functional Anatomy, Faculty of Agriculture, Shinshu University, Nagano-ken, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hiramatsu
authors.forename: K
authors.initials: K
authors.lastname: Ohshima
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D019004:Galanin; D005724:Ganglia / Q000166:cytology / Q000378:metabolism; D006651:Histocytochemistry; D008297:Male; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D010179:Pancreas / Q000294:innervation*; D010455:Peptides / Q000378:metabolism; D014018:Tissue Distribution; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
chemical_list: D009479:Neuropeptides; D010455:Peptides; D014660:Vasoactive Intestinal Peptide; D019004:Galanin; D009252:NADPH Dehydrogenase