[Evaluation of hemoglobin dextran 10-benzene-tetracarboxylate, oxygen transporters, using a model of guinea pig intestine].
With the aim of assessing of dextran-benzene-tetracarboxylate hemoglobin as an oxygen carrier, we studied histological changes in the intestinal loop. The intestinal tissue being very sensitive to hypoxia, in the anesthetized guinea pig, an innervated loop was vascularly perfused with open-flow during one hour at zero hematocrit. To estimate the capacity of hemoglobin solution to oxygenate this tissue, we observed the mechanical and histological changes in the organ and the arterio-venous difference in PO2, oxyhemoglobin, deoxyhemoglobin and we compared them with human albumin, Tyrode and non-modified hemoglobin. The PO2 arterio-venous differences were 51.9 +/- 7.1 torr (m +/- SEM) for Tyrode, 40.2 +/- 6.4 torr for albumin solution, 113.7 +/- 6.5 torr for non-modified hemoglobin and 123.1 +/- 7.9 torr for dex-BTC-Hb. Compared to albumin and Tyrode solutions, hemoglobin solutions transferred more oxygen to tissues. The desaturation of dex-BTC-Hb was significantly superior (p < 0.05) to the one non-modified hemoglobin. The structure of jejunal villi when perfused with a hemoglobin solution, remained almost normal and the loop was still active. Nevertheless, non-modified hemoglobin leaked from the vessels to the lumen and caused oedema and a rupture of overlapping epithelium at the tip of the villi. With dex-BTC-Hb, such histological modifications were less significant. With albumin and Tyrode, all villi were totally necrosed and the loop was completely inert. We have demonstrated that dextran-benzene-tetracarboxylate hemoglobin had the ability to maintain the tissue alive thank to its good capacity to release oxygen and its satisfactory vascular persistence.
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Laboratoire d'Hématologie et Physiologie, Faculté des Sciences pharmaceutiques et biologiques, Nancy.
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Labrude
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Vigneron
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D000818:Animals; D001565:Benzoates / Q000737:chemistry / Q000378:metabolism*; D001692:Biological Transport; D003911:Dextrans / Q000737:chemistry / Q000378:metabolism*; D006168:Guinea Pigs; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism*; D007583:Jejunum / Q000033:anatomy & histology / Q000378:metabolism* / Q000502:physiology; D008297:Male; D008954:Models, Biological; D009119:Muscle Contraction; D010100:Oxygen / Q000097:blood*; D010101:Oxygen Consumption
D001565:Benzoates; D003911:Dextrans; D006454:Hemoglobins; C095659:hemoglobin-dextran 10-benzene-tetracarboxylate; D010100:Oxygen