Changes in tissue distribution of rat alpha 1-macroglobulin and pregnancy-associated alpha 1-glycoprotein after inflammatory injury.
Antiserum against rat alpha 1-macroglobulin (alpha 1MG) was produced in rabbits. Antiserum against rat pregnancy-associated alpha 1-glycoprotein (PAG) was obtained by immunization with a partly purified PAG preparation and absorption of the serum with male rat serum. Acute inflammation was produced in non-pregnant female rats by a single intramuscular injection of turpentine. The concentrations of both macroglobulins in the serum and in tissue extracts were measured by rocket immunoelectrophoresis at various times up to 7 days after injury. Inflammation produced in the rats resulted in moderately elevated serum levels of these proteins soon after injury. At first, alpha 1MG levels in a number of tissues (heart, lung, kidney, spleen, pancreas, uterus and ovary) were depressed markedly; they then stabilized. The elevated serum concentrations of alpha 1MG remained unchanged during inflammation. The store of PAG in the tissues was rapidly depleted and its serum level decreased to a normal value 7 days after injury. Our findings indicate that alpha 1MG plays a more important role in maintenance of the proteinase inhibitory potential in the rat than does PAG.
Zorin
N A
NA
Central Research Laboratory, Postgraduate Physician Training Institute, Novokuznetsk, Russia.
Zhabin
S G
SG
Belogorlova
T I
TI
Chirikova
T S
TS
Krayushkina
N A
NA
Lykova
O F
OF
D000210:Acute-Phase Reaction / Q000097:blood / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000097:blood / Q000378:metabolism*; D002846:Chromatography, Affinity; D005260:Female; D007106:Immune Sera; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D011257:Pregnancy Proteins / Q000097:blood / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D014425:Turpentine; D000511:alpha-Macroglobulins / Q000302:isolation & purification / Q000378:metabolism*
D002352:Carrier Proteins; D007106:Immune Sera; D011257:Pregnancy Proteins; D000511:alpha-Macroglobulins; C489170:pregnancy-associated alpha 1-glycoprotein, rat; D014425:Turpentine