title: Ex vivo hematopoietic progenitor cell expansion.
abstract: The ability to culture and expand hematopoietic progenitor cells ex vivo has major implications for both bone marrow and stem cell support following marrow ablative or subablative high-dose therapy and for improving the efficiency of retroviral transfection in gene marking and gene therapy. This review focuses on methods for the generation of myeloid progenitor and post-progenitor cells from peripheral blood stem cell collections, with particular emphasis on the characterization of these cells and practical issues associated with their expansion.
authors.affiliation: Leukaemia Research Unit, Hanson Centre for Cancer Research, Adelaide, Australia.
authors.forename: D N
authors.initials: DN
authors.lastname: Haylock
authors.forename: S
authors.initials: S
authors.lastname: Makino
authors.forename: T L
authors.initials: TL
authors.lastname: Dowse
authors.forename: S
authors.initials: S
authors.lastname: Trimboli
authors.forename: S
authors.initials: S
authors.lastname: Niutta
authors.forename: L B
authors.initials: LB
authors.lastname: To
authors.forename: C A
authors.initials: CA
authors.lastname: Juttner
authors.forename: P J
authors.initials: PJ
authors.lastname: Simmons
mesh_terms: D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D002469:Cell Separation; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D006410:Hematopoiesis / Q000502:physiology*; D006801:Humans; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34