Liquid chromatographic determination of sulfadiazine in salmon by postcolumn derivatization and fluorescence detection.
A reversed-phase (ODS-2) liquid chromatographic method was developed to determine low nanogram-per-gram levels of sulfadiazine (SDZ) in salmon muscle tissue. SDZ was extracted with acetonitrile-aqueous 2% acetic acid (pH 3.0), partitioned into methylene chloride, and cleaned up by using a strong-cation-exchange, solid-phase extraction cartridge. SDZ was derivatized postcolumn with fluorescamine and detected by fluorescence. The limit of detection was 0.2 ng SDZ/g tissue. Recoveries from coho salmon tissue fortified with 1, 5, 10, and 20 ng SDZ/g tissue averaged 84.5, 85.0, 83.6, and 83.9%, respectively; recoveries from Atlantic salmon tissue fortified with 10 ng SDZ/g tissue averaged 82.6%.
Gehring
T A
TA
U.S. Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR 72079, USA.
Rushing
L G
LG
Thompson
H C
HC
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods* / Q000706:statistics & numerical data; D005450:Fluorescamine; D005456:Fluorescent Dyes; D008832:Microchemistry; D012474:Salmon; D017747:Seafood / Q000032:analysis*; D012680:Sensitivity and Specificity; D013411:Sulfadiazine / Q000032:analysis*
D000890:Anti-Infective Agents; D005456:Fluorescent Dyes; D013411:Sulfadiazine; D005450:Fluorescamine