PCR expression analysis of the estrogen-inducible gene BCEI in gastrointestinal and other human tumors.
A polymerase chain reaction (PCR) assay was developed to test for tumor cell specific expression of the BCEI gene. This new marker gene, reported at first for human breast cancer, was found specifically active in various gastrointestinal carcinomas by previously applying immunohistochemistry and RNA (Northern blot) analysis. Presently, by using reverse transcription-PCR analysis, a series of primary tumor tissues and established tumor cell lines were tested for BCEI transcription. This approach was compared to immunostaining achieved by an antibody directed against the BCEI gene's product. The result demonstrate the superior sensitivity of PCR by indicating the gene's expression in cases where immunohistochemical testing remained negative.
Wundrack
I
I
Div. of Molecular Genetics, University of Tübingen, Germany.
Müllenbach
R
R
Welter
C
C
Seitz
G
G
Blin
N
N
D001483:Base Sequence; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000235:genetics; D004967:Estrogens / Q000494:pharmacology*; D005770:Gastrointestinal Neoplasms / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011506:Proteins; D012194:RNA-Directed DNA Polymerase; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins
D004967:Estrogens; D005819:Genetic Markers; D009363:Neoplasm Proteins; D011506:Proteins; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins; D012194:RNA-Directed DNA Polymerase