title: Novel three-dimensional 1H-13C-31P triple resonance experiments for sequential backbone correlations in nucleic acids.
abstract: Backbone-driven assignment methods that utilize covalent connectivities have greatly facilitated spectral assignments of proteins. In nucleic acids, 1H-13C-31P correlations could play a similar role, and several related experiments (HCP) have recently been presented for backbone-driven sequential assignments in RNA. The three-dimensional extension of 1H-31P Het-Cor (P,H-COSY-H,C-HMQC) and Het-TOCSY (P,H-TOCSY-H,C-HMQC) experiments presented here complements HCP experiments as tools for spectral assignments and extraction of dihedral angle constraints. By relying on 1H-31P rather than 13C-31P couplings to generate cross peaks, the strongest connectivities are observed in different spectral regions, increasing the likelihood of resolving spectral overlap. In addition, semiquantitative estimates of 1H-31P and 13C-31P couplings provide dihedral angle constraints for RNA structure determination.
authors.affiliation: MRC Laboratory of Molecular Biology, Cambridge, U.K.
authors.forename: G
authors.initials: G
authors.lastname: Varani
authors.forename: F
authors.initials: F
authors.lastname: Aboul-ela
authors.forename: F
authors.initials: F
authors.lastname: Allain
authors.forename: C C
authors.initials: CC
authors.lastname: Gubser
mesh_terms: D001483:Base Sequence; D002247:Carbon Isotopes; D006859:Hydrogen; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry*; D010759:Phosphorus Isotopes; D012313:RNA / Q000737:chemistry
chemical_list: D002247:Carbon Isotopes; D009696:Nucleic Acids; D010759:Phosphorus Isotopes; D012313:RNA; D006859:Hydrogen