title: Influence of various immunosuppressive agents on the occurrence of endogenous bacteraemia in mice.
abstract: The influence of six immunosuppressive agents on the occurrence of endogenous bacteraemia in mice was evaluated. The mortality rates in conventional ddY mice given cyclophosphamide (CY), fluorouracil (5-FU), methotrexate (MTX), cisplatin (CDDP) or FK-506 intraperitoneally, or dexamethasone (DXM) subcutaneously were 70, 100, 100, 100, 0 and 0%, respectively. Pseudomonas aeruginosa was isolated from 70% of mice treated with CY but from only 10% of mice treated with 5-FU and 30% treated with MTX. Enterobacteria were isolated from 90% of mice treated with 5-FU. Specific-pathogen-free (SPF) mice fed P. aeruginosa were also treated with these agents. All mice in the CY, 5-FU, MTX and CDDP groups died whereas mice treated with DXM and FK-506 showed 20% and 0% mortality, respectively. Pure cultures of P. aeruginosa were obtained from all of the mice treated with CY. Polymicrobial bacteraemia with P. aeruginosa and enterobacteria occurred in 5, 25, 5 and 5% of mice treated with 5-FU, MTX, CDDP and DXM, respectively. Enterobacterial bacteraemia was observed in 70% of mice treated with CDDP and in 5% of the DXM group. Different types of bacteraemia were induced by different immunosuppressive agents. The mechanism of immunosuppression may affect the frequency of bacteraemia and the causative organism.
authors.affiliation: Department of Microbiology, Toho University School of Medicine, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hirakata
authors.forename: N
authors.initials: N
authors.lastname: Furuya
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: K
authors.initials: K
authors.lastname: Tateda
authors.forename: K
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000209:etiology* / Q000401:mortality; D002945:Cisplatin / Q000633:toxicity; D003520:Cyclophosphamide / Q000633:toxicity; D004756:Enterobacteriaceae Infections / Q000209:etiology* / Q000401:mortality; D005472:Fluorouracil / Q000633:toxicity; D007166:Immunosuppressive Agents / Q000633:toxicity*; D008297:Male; D008727:Methotrexate / Q000633:toxicity; D051379:Mice; D011552:Pseudomonas Infections / Q000209:etiology* / Q000401:mortality; D013047:Specific Pathogen-Free Organisms; D016559:Tacrolimus / Q000633:toxicity
chemical_list: D007166:Immunosuppressive Agents; D003520:Cyclophosphamide; D002945:Cisplatin; D005472:Fluorouracil; D016559:Tacrolimus; D008727:Methotrexate