title: In vitro and ex vivo expression of nucleolar proteins B23 and p120 in benign and malignant epithelial lesions of the prostate.
abstract: The expression of two specific nucleolar antigens, p120 and B23, has been investigated in the prostatic carcinoma cell line LNCaP as well as in 40 frozen and 40 formalin-fixed tissue samples of benign and malignant prostatic lesions (15 benign hyperplasias, 5 grade 1, 15 grade 2, and 5 grade 3 carcinomas). In vitro, immunoreactivity of p120 was confined to nucleoli of proliferating cells, with virtually no negative staining during S and G2/M phases. Unlike p120, B23 was expressed in the nucleoli of all LNCaP cells independently of growth and cell cycle phases. Hence, B23 was detectable in all stromal as well as in normal and malignant epithelial prostatic cells, both in fresh and in formalin-fixed tissue sections after microwave treatment. In contrast, the immunoreactivity of p120 was almost completely restricted to the nucleoli of prostate carcinoma cells: frozen sections of benign prostatic hyperplasia (n = 15) were either totally negative for p120 (n = 13) or had a low percentage of positively stained cells (labeling index = 3.3% in 3 cases). In the carcinoma group 76% (19/25) of the specimens were p120 positive, and there was a significant rise of labeling index from 18.1% in grade 1 to 82.2% in grade 3 carcinomas (P < 0.001). In contrast to B23, p120 could not be reliably demonstrated in formalin-fixed and paraffin-embedded tissue. We therefore conclude that anti-B23 is a general marker of nucleoli, whereas expression of p120 appears to correlate with "hyperactivity" of the nucleolus and provides a new tool for flow cytometrical and immunohistochemical assessment of nucleolar activity in tumor pathology.
authors.affiliation: Institute of Pathology, University of Regensburg, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Bocker
authors.forename: A
authors.initials: A
authors.lastname: Bittinger
authors.forename: W
authors.initials: W
authors.lastname: Wieland
authors.forename: R
authors.initials: R
authors.lastname: Buettner
authors.forename: G
authors.initials: G
authors.lastname: Fauser
authors.forename: F
authors.initials: F
authors.lastname: Hofstaedter
authors.forename: J
authors.initials: J
authors.lastname: Rüschoff
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000096:biosynthesis; D014408:Biomarkers, Tumor; D002453:Cell Cycle; D002455:Cell Division; D002466:Cell Nucleolus / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004848:Epithelium; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000096:biosynthesis*; D009697:Nucleolus Organizer Region / Q000378:metabolism; D000090243:Nucleophosmin; D010750:Phosphoproteins / Q000096:biosynthesis; D011470:Prostatic Hyperplasia / Q000378:metabolism* / Q000473:pathology; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured; D012359:tRNA Methyltransferases
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D009687:Nuclear Proteins; D010750:Phosphoproteins; D000090243:Nucleophosmin; C065028:NOP2 protein, human; D012359:tRNA Methyltransferases