Chemistry and applications of oligonucleotide analogues.
This review is aimed at biochemists and molecular biologists, and covers the chemistry and key features involved in the solid-phase synthesis of a variety of the better known DNA and RNA analogues by the phosphoramidite and H-phosphonate methods. A wide spectrum of biological applications such as inhibition of gene expression, translation arrest, RNA processing, affinity purification of RNA-protein complexes, in situ hybridization, and synthetic ribozymes are then discussed in some detail, enabling the molecular biologist to get an idea of what is possible using the current technology.
Sproat
B S
BS
Ribonetics GmbH, Göttingen, Germany.
D000818:Animals; D004247:DNA / Q000737:chemistry; D015870:Gene Expression; D006801:Humans; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D063065:Organophosphonates; D012313:RNA / Q000737:chemistry; D013873:Thionucleotides
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D063065:Organophosphonates; D013873:Thionucleotides; D012313:RNA; D004247:DNA