title: A novel p16INK4A transcript.
abstract: p16INK4A and p15INK4B were initially identified as potent inhibitors of activated cyclin/cyclin-dependent kinase complexes. These genes were colocalized to chromosome 9p21, and p16 was subsequently found to be mutated in familial melanoma and deleted in a wide variety of sporadic cancers. We recently found that de novo methylation of a 5' CpG island led to transcriptional block of full-length p16 in many neoplasms. However, the presence of a truncated p16 transcript in methylated cell lines led us to investigate the presence of an alternative promoter or initiation site. We have now identified an abundant alternative p16 transcript in both methylated and unmethylated cell lines generated from a novel sequence (exon 1 beta) potentially involved in the complex regulation of these critical cell cycle genes.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2196, USA.
authors.forename: L
authors.initials: L
authors.lastname: Mao
authors.forename: A
authors.initials: A
authors.lastname: Merlo
authors.forename: G
authors.initials: G
authors.lastname: Bedi
authors.forename: G I
authors.initials: GI
authors.lastname: Shapiro
authors.forename: C D
authors.initials: CD
authors.lastname: Edwards
authors.forename: B J
authors.initials: BJ
authors.lastname: Rollins
authors.forename: D
authors.initials: D
authors.lastname: Sidransky
mesh_terms: D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002899:Chromosomes, Human, Pair 9; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D005091:Exons; D005784:Gene Amplification; D016147:Genes, Tumor Suppressor; D006801:Humans; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000235:genetics; D016133:Polymerase Chain Reaction; D012313:RNA / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D002352:Carrier Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm; D012313:RNA