Sequence analysis of cohesive ends of the actinophage RP3 genome and construction of a transducible shuttle vector.
The sequence of the cohesive ends of actinophage RP3 DNA has been determined. As with all other phages of Gram-positive bacteria that have been studied sofar, RP3 DNA has 3'-protruding ends. A shuttle cosmid has been constructed containing this cos area which can be efficiently transduced by phage RP3 to host cells of Streptomyces rimosus.
Kinner
E
E
Institut für Genetik und Mikrobiologie, Universität München, FRG.
Pocta
D
D
Ströer
S
S
Schmieger
H
H
D001435:Bacteriophages / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D016679:Genome, Viral; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013302:Streptomyces; D014161:Transduction, Genetic