PTB domain binding to signaling proteins through a sequence motif containing phosphotyrosine.
Src homology 2 (SH2) domains mediate assembly of signaling complexes by binding specifically to tyrosine-phosphorylated proteins. A phosphotyrosine binding (PTB) domain has been identified which also binds specifically to tyrosine-phosphorylated targets, but is structurally different from SH2 domains. Expression cloning was used to identify targets of PTB domains. PTB domains bound to phosphotyrosine within a sequence motif, asparagine-X1-X2-phosphotyrosine (where X represents any amino acid), that is found in many signaling proteins and is not recognized by SH2 domains. Mutational studies indicated that high affinity binding of PTB domains may require a specific conformation of the motif.
Kavanaugh
W M
WM
Department of Medicine, University of California, San Francisco 94143, USA.
Turck
C W
CW
Williams
L T
LT
D000595:Amino Acid Sequence; D001665:Binding Sites / Q000502:physiology; D001667:Binding, Competitive; D002460:Cell Line; D006801:Humans; D008969:Molecular Sequence Data; D010748:Phosphopeptides / Q000378:metabolism*; D019000:Phosphotyrosine; D011485:Protein Binding / Q000502:physiology*; D011994:Recombinant Proteins / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D010748:Phosphopeptides; D011994:Recombinant Proteins; D019000:Phosphotyrosine; D014443:Tyrosine