Transient transfection assays to examine the requirement of putative cell death genes.
In conclusion, this chapter provides a convenient and efficient method for the detection and analysis of transiently transfected cells. Such strategies allow a fast and simple analysis of the requirement for particular genes that have been identified as being induced during apoptosis. Our experience has been that, when screening for "cell death genes," it is easy to isolate genes induced during apoptosis but far more difficult to determine the requirement for any given gene. These protocols have rendered such determinations much simpler to perform.
Osborne
B A
BA
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst 01003, USA.
Smith
S W
SW
Liu
Z G
ZG
McLaughlin
K A
KA
Schwartz
L M
LM
D015703:Antigens, CD / Q000032:analysis / Q000235:genetics*; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis / Q000235:genetics*; D017209:Apoptosis / Q000235:genetics*; D015415:Biomarkers; D002469:Cell Separation / Q000379:methods; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D006825:Hybridomas; D016376:Oligonucleotides, Antisense / Q000235:genetics; D011205:Povidone; D011994:Recombinant Proteins / Q000032:analysis; D012822:Silicon Dioxide; D013601:T-Lymphocytes; D014162:Transfection / Q000379:methods*
D015703:Antigens, CD; D018951:Antigens, CD20; D000944:Antigens, Differentiation, B-Lymphocyte; D015415:Biomarkers; D016376:Oligonucleotides, Antisense; D011994:Recombinant Proteins; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone