Clinical evaluation of the slide centrifuge (cytospin) gram's stained smear for the detection of bacteriuria and comparison with the FiltraCheck-UTI and UTIscreen.
The cytospin Gram's stained smear method for the detection of bacteriuria was compared with the FiltraCheck-UTI, the UTIscreen and culture of urine specimens from adult male and female inpatients and outpatients in a tertiary care medical institution. At a level of > or = 10(5) CFU/mL, the sensitivity of all three methods was at least 93% for organisms identified as potential pathogens. Investigation of false-negative screening tests by chart review demonstrated that only 5 of 10 combined misses in this category were clinically significant. At a level of > or = 10(4) CFU/mL, the sensitivity of the cytospin method remained essentially unchanged, whereas that of the FiltraCheck-UTI and the UTIscreen decreased to 89% and 85%, respectively. False positives were most frequent with the FiltraCheck-UTI, which was due in part to the presence of white blood cells in the urine. All three tests performed well in this population of patients.
Rippin
K P
KP
Department of Clinical Pathology, Cleveland Clinic Foundation, Ohio 444195-5140.
Stinson
W C
WC
Eisenstadt
J
J
Washington
J A
JA
D000328:Adult; D001431:Bacteriological Techniques / Q000295:instrumentation*; D001437:Bacteriuria / Q000382:microbiology* / Q000652:urine; D002498:Centrifugation; D015169:Colony Count, Microbial; D004755:Enterobacteriaceae / Q000302:isolation & purification; D016983:Enterococcus / Q000302:isolation & purification; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D005374:Filtration; D005840:Gentian Violet; D006801:Humans; D008297:Male; D010619:Phenazines; D011237:Predictive Value of Tests; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D016482:Urinalysis / Q000295:instrumentation / Q000379:methods*; D014556:Urine / Q000382:microbiology*; D015003:Yeasts / Q000302:isolation & purification
C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet