Detection of proteins and sialoglycoproteins in polyacrylamide gels using eosin Y stain.
An eosin Y staining technique that permits detection of various proteins, including membrane sialoglycoproteins, in polyacrylamide gels is described. The sensitivity of the eosin Y staining method is comparable to silver staining. In addition, there is an added advantage of the antigenicity of the stained proteins being retained in a Western blot. Details of the procedure to obtain optimal staining results are described.
Lin
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Department of Anatomy and Cell Biology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.
Wise
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GE
D000941:Antigens; D001709:Biotechnology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D004801:Eosine Yellowish-(YS); D008565:Membrane Proteins / Q000302:isolation & purification; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification*; D012795:Sialoglycoproteins / Q000302:isolation & purification*; D012834:Silver; D013194:Staining and Labeling / Q000379:methods
D000941:Antigens; D008565:Membrane Proteins; D011506:Proteins; D012795:Sialoglycoproteins; D012834:Silver; D004801:Eosine Yellowish-(YS)