title: Ca(2+)-independent nitric oxide synthase activity in human lung after cardiopulmonary bypass.
abstract: BACKGROUND
  Because surgery involving cardiopulmonary bypass induces a systemic inflammatory response, the effect of cardiopulmonary bypass on nitric oxide (NO) generation was investigated in human lung tissue.
  
  
  METHODS
  Nitric oxide synthase (NOS) activity was measured by the conversion of 14C-L-arginine to 14C-L-citrulline in tissue biopsy samples obtained before and after cardiopulmonary bypass.
  
  
  RESULTS
  The Ca(2+)-independent production of NO found before cardiopulmonary bypass was extremely low (1.5 (0.5) pmol citrulline/mg/min), but was increased after the bypass operation (23.6 (11) pmol/mg/min).
  
  
  CONCLUSIONS
  Ca(2+)-independent NOS activity was detected in human lung after cardiopulmonary bypass. This finding may provide an important insight into the pathogenesis of the tissue damage and acute phase response observed after such surgery.
authors.affiliation: Center of Pharmaceutical Chemistry, Havana, Cuba.
authors.forename: R
authors.initials: R
authors.lastname: Delgado
authors.forename: A
authors.initials: A
authors.lastname: Rojas
authors.forename: L A
authors.initials: LA
authors.lastname: Glaría
authors.forename: M
authors.initials: M
authors.lastname: Torres
authors.forename: F
authors.initials: F
authors.lastname: Duarte
authors.forename: R
authors.initials: R
authors.lastname: Shill
authors.forename: M
authors.initials: M
authors.lastname: Nafeh
authors.forename: E
authors.initials: E
authors.lastname: Santin
authors.forename: N
authors.initials: N
authors.lastname: González
authors.forename: M
authors.initials: M
authors.lastname: Palacios
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000032:analysis* / Q000096:biosynthesis; D001120:Arginine / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002315:Cardiopulmonary Bypass; D002956:Citrulline / Q000378:metabolism; D006331:Heart Diseases / Q000201:enzymology / Q000601:surgery; D006801:Humans; D008168:Lung / Q000201:enzymology*; D019001:Nitric Oxide Synthase; D011184:Postoperative Period
chemical_list: D002956:Citrulline; D001120:Arginine; D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D002118:Calcium