title: An operon encoding a novel ABC-type transport system in Bacillus subtilis.
abstract: Downstream from the surfactin synthetase operon in Bacillus subtilis a new operon-type structure has been localized which, on the basis of sequence determination, potentially encodes an ABC-type transport system. The 268 amino acid protein, the product of orf1, represents the solute-binding component of the system whereas the orf2 product, a 234 amino acid protein, is the transmembrane component. Finally orf3 potentially encodes a typical 241 amino acid ATP-binding protein involved in energy supply. Comparison of the three proteins with the subunits of other ABC-type systems suggests that this new system is involved in amino acid transport.
authors.affiliation: ENIRICERCHE S.p.A. - Genetic Engineering and Microbiology Laboratories,Milan, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Rodriguez
authors.forename: G
authors.initials: G
authors.lastname: Grandi
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D010453:Peptide Synthases / Q000235:genetics; D015183:Restriction Mapping; D017855:Transferases (Other Substituted Phosphate Groups)
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D002352:Carrier Proteins; C082662:phosphopantetheinyl transferase; D017855:Transferases (Other Substituted Phosphate Groups); D010453:Peptide Synthases; C069542:surfactin synthetase