title: Differentiation of human trophoblast populations involves alterations in cytokeratin patterns.
abstract: Cytokeratins (CKs) are related to proliferation and differentiation of epithelial cells. Little knowledge exists about CK patterns in human trophoblast subpopulations (villous and extravillous trophoblasts). To better understand differentiation and function of trophoblast components, we studied the distribution patterns of CKs in the placenta throughout pregnancy. A panel of well-defined monoclonal antibodies against different types of cytokeratins, vimentin, and fibrin, was used on frozen and paraffin sections. CK8, 18, and 19 were expressed in all the villous and extravillous trophoblastic subsets throughout pregnancy. In the first trimester, syncytiotrophoblasts were positive for CK7 and 13 along the basal membrane. As pregnancy progressed there was an increase in intensity of the reaction product and a more diffuse positive staining of CK7 in the cytoplasm of the syncytium, with evident positivity along the apical membrane. CK13 showed similar expression as CK7, but with less intense staining along the apical membrane and less prominent staining in the cytoplasm. Villous cytotrophoblasts were also positive for CK7 and CK13. CK17 was found related to cytotrophoblastic cells in contact with or next to fibrin deposits. Extravillous cytotrophoblasts in cell islands and cell columns were positive for CK13 only in the cell layers located proximal to the villous stroma, whereas the distal and more differentiated cells were negative. CK7 was positive in all epithelial cells of cell islands and columns, but the reaction product was not present in cells deeply migrated into the decidua. Amnion was negative for anti-CK13 antibodies in the first trimester but was positive at term. CK4 and CK16 were not found in the placenta. Our study shows for the first time that the different populations of human placental trophoblast express cytokeratins in developmental, differentiative, and functional specific patterns. These findings can be useful to distinguish and classify the various trophoblastic populations and provide a foundation for studying pathological aspects of the trophoblast.
authors.affiliation: Laboratory of Vascular Pathology, Istituto Dermopatico dell'Immacolata, IDI, Rome, Italy.
authors.forename: J
authors.initials: J
authors.lastname: Mühlhauser
authors.forename: C
authors.initials: C
authors.lastname: Crescimanno
authors.forename: M
authors.initials: M
authors.lastname: Kasper
authors.forename: D
authors.initials: D
authors.lastname: Zaccheo
authors.forename: M
authors.initials: M
authors.lastname: Castellucci
mesh_terms: D002454:Cell Differentiation; D005260:Female; D005337:Fibrin / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D007633:Keratins / Q000032:analysis*; D011247:Pregnancy / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*; D014746:Vimentin / Q000032:analysis
chemical_list: D014746:Vimentin; D007633:Keratins; D005337:Fibrin