title: Dopamine and serotonin turnover rate in the retina of rabbit, rat, goldfish, and Eugerres plumieri: light effects in goldfish and rat.
abstract: The concentration of dopamine, and its metabolites 3,4-dihydroxyphenylacetic and homovanillic acids, as well as serotonin and its metabolite 5-hydroxyindoleacetic acid, were determined in the retina of two teleosts, C. auratus (goldfish) and E. plumieri (mojarra), and two mammals, R. norvegicus (rat) and O. cuniculus (rabbit). The turnover rate of these monoamines were investigated in the four species by the calculation of the ratio monoamine/metabolite as an indirect index, and in goldfish and rat by the inhibition of the synthesis with alpha-methyl-p-tyrosine or p-chlorophenylalanine, by the increase in dopamine or serotonin by the corresponding precursors, 3,4-dihydroxyphenylalanine or 5-hydroxytryptophan, and by inhibition of monoaminooxidase with pargyline. The modulation by light and dark stimulation was studied in the goldfish and the rat. Differences in the concentration and turnover rate were observed among the species. Serotonin concentration was higher in the teleosts. The administration of inhibitors of dopamine and serotonin synthesis differentially decreased the levels of the monoamines in the retina of goldfish and rat. The rate of formation of dopamine and serotonin by the corresponding precursors was much higher in the goldfish than in the rat. Pargyline administration decreased 3,4-dihydroxyphenylacetic and 5-hydroxyindoleacetic acids at different rates and time dependency in the retina of goldfish and rat. Dopamine and serotonin concentration did not exhibit high modifications by the inhibitor, suggesting the function of regulatory mechanisms or additional effect of pargyline at other sites different from monoaminooxidase.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratorio de Neuroquímica, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
authors.forename: L
authors.initials: L
authors.lastname: Lima
authors.forename: M
authors.initials: M
authors.lastname: Urbina
mesh_terms: D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D006916:5-Hydroxytryptophan / Q000494:pharmacology; D000818:Animals; D002230:Carbidopa / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D010134:Fenclonine / Q000494:pharmacology; D006054:Goldfish / Q000378:metabolism*; D006719:Homovanillic Acid / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007980:Levodopa / Q000494:pharmacology; D008027:Light; D008297:Male; D008781:Methyltyrosines / Q000494:pharmacology; D010293:Pargyline / Q000494:pharmacology; D010473:Perciformes / Q000378:metabolism*; D011817:Rabbits / Q000378:metabolism*; D051381:Rats / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism* / Q000528:radiation effects; D012701:Serotonin / Q000378:metabolism*; D013045:Species Specificity; D019805:alpha-Methyltyrosine
chemical_list: D008781:Methyltyrosines; D015102:3,4-Dihydroxyphenylacetic Acid; D012701:Serotonin; D007980:Levodopa; D006897:Hydroxyindoleacetic Acid; D019805:alpha-Methyltyrosine; D010293:Pargyline; D006916:5-Hydroxytryptophan; D002230:Carbidopa; D010134:Fenclonine; D004298:Dopamine; D006719:Homovanillic Acid