Gender-related differences in expression of murine glutathione S-transferases and their induction by butylated hydroxyanisole.
The basal levels of mu and pi class glutathione S-transferases RNA were 18-fold higher in the male mouse liver as compared with the female. When 0.75% (w/w) BHA was included in the diet it altered the RNA levels of alpha, mu, pi GST classes and mGSTA4-4 in a tissue and sex specific manner. The most marked induction of RNA was seen for the mu class GSTs of female liver, lung and kidney (52, 10 and 8-fold, respectively), and of male liver and kidney (25 and 3.5-fold, respectively), the pi class GSTs of female liver, lung, and kidney (11, 10, and 5-fold, respectively), and mGSTA4-4 of female liver (4-fold). The effect of BHA on the induction of the mu and pi class GST RNA was 2-9 fold greater in female as compared with male tissues. The degree of induction of GST RNA did not correlate directly with changes in GST protein indicating that post-transcriptional events regulating GST expression may be affected by BHA particularly for GST mu and mGSTA4-4.
Chaubey
M
M
Department of Human Biological Chemistry, University of Texas Medical Branch, Galveston 77555.
Singhal
S S
SS
Awasthi
S
S
Saxena
M
M
Dyer
R B
RB
Awasthi
Y C
YC
Herzog
N K
NK
D000818:Animals; D002083:Butylated Hydroxyanisole / Q000494:pharmacology*; D004790:Enzyme Induction; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D012727:Sex Characteristics
D007527:Isoenzymes; D002083:Butylated Hydroxyanisole; D012313:RNA; D005982:Glutathione Transferase