title: Etiology of cat scratch disease: comparison of polymerase chain reaction detection of Bartonella (formerly Rochalimaea) and Afipia felis DNA with serology and skin tests.
abstract: To determine the role of Bartonella (formerly Rochalimaea) species and Afipia felis in cat scratch disease (CSD), two polymerase chain reaction (PCR) hybridization assays were developed to detect DNA from these organisms. These assays were applied on 89 pus aspirates from skin test-positive CSD patients (group 1) and on 137 pus and lymph node specimens from CSD suspects (group 2). Bartonella DNA was detected in 96% of the samples from group 1 patients and in 60% of group 2 samples; however, A. felis DNA could not be detected in any clinical samples. These results suggest that CSD is caused by bartonellae and that A. felis does not play a significant role in this zoonosis. A strong correlation between Bartonella PCR positivity and Bartonella henselae antibody titer was found. Comparison of CSD skin test results with those obtained by Bartonella PCR suggests a low sensitivity of the skin test.
authors.affiliation: Molecular Microbiology Unit, National Institute of Public Health and Environmental Protection, Bilthoven, Netherlands.
authors.forename: A M
authors.initials: AM
authors.lastname: Bergmans
authors.forename: J W
authors.initials: JW
authors.lastname: Groothedde
authors.forename: J F
authors.initials: JF
authors.lastname: Schellekens
authors.forename: J D
authors.initials: JD
authors.lastname: van Embden
authors.forename: J M
authors.initials: JM
authors.lastname: Ossewaarde
authors.forename: L M
authors.initials: LM
authors.lastname: Schouls
mesh_terms: D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000097:blood; D018416:Bartonella henselae / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D002372:Cat-Scratch Disease / Q000382:microbiology*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012882:Skin Tests; D013492:Suppuration / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S