Imaging of DNA in human and plant chromosomes by high-resolution scanning electron microscopy.
We describe a DNA-specific staining procedure, using a blue platinum organic dye, which allows DNA imaging of chromosomes by detection of back-scattered electrons in the scanning electron microscope. DNA distribution is visualized within chromosomal details of the centromeric and satellite regions, or in interphase chromatin, with high-resolution (10-15 nm) for comparison with the corresponding secondary electron image representing DNA plus protein.
Wanner
G
G
Botanisches Institut, Universität München, Germany.
Formanek
H
H
D002503:Centromere / Q000235:genetics; D002712:Chlorides; D002843:Chromatin / Q000235:genetics; D002875:Chromosomes / Q000235:genetics* / Q000648:ultrastructure; D002877:Chromosomes, Human / Q000235:genetics* / Q000648:ultrastructure; D004247:DNA / Q000032:analysis*; D018744:DNA, Plant / Q000032:analysis*; D005976:Glutaral; D006801:Humans; D007399:Interphase; D008677:Metaphase; D008855:Microscopy, Electron, Scanning; D009942:Organometallic Compounds; D009993:Osmium Tetroxide; D010944:Plants / Q000235:genetics*; D017671:Platinum Compounds; D013194:Staining and Labeling
D002712:Chlorides; D002843:Chromatin; D018744:DNA, Plant; D009942:Organometallic Compounds; D017671:Platinum Compounds; C036919:tetrachloroplatinate; C005460:uranyl acetate; D004247:DNA; D009993:Osmium Tetroxide; D005976:Glutaral