Differential expression of the H-ras mutated and normal alleles in rabbit DMBA-induced keratoacanthomas.
Keratoacanthomas (KAs) are benign and self-regressing tumors in which a high incidence of the mutated H-ras oncogene has been observed both in humans and in experimental models. To determine the level of expression of the mutated H-ras allele with respect to its normal counterpart in 7,12-dimethylbenz(a)anthracene (DMBA)-induced KAs in rabbit skin, we have utilized a quantitative technique based on reverse transcription polymerase chain reaction (RT-PCR) and selective cleavage of the mutated molecules of the H-ras gene. Analysis of 16 KAs showed that the mutated H-ras transcripts were up to 3-fold more abundant than the non-mutated H-ras transcript in the different tumors. This higher expression of the mutated allele appears to correlate with increased differentiation in the KAs and in turn may contribute to tumor regression.
Matesanz
F
F
Department of Pathology, New York University Medical Center, NY 10016, USA.
Oliva
M R
MR
Villamarin
A
A
Kamino
H
H
Pellicer
A
A
D015127:9,10-Dimethyl-1,2-benzanthracene; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D011905:Genes, ras; D007636:Keratoacanthoma / Q000139:chemically induced / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D012194:RNA-Directed DNA Polymerase; D011817:Rabbits; D012878:Skin Neoplasms / Q000139:chemically induced / Q000235:genetics*
D015127:9,10-Dimethyl-1,2-benzanthracene; D012194:RNA-Directed DNA Polymerase