title: Chicken interferon-mediated induction of major histocompatibility complex class II antigens on peripheral blood monocytes.
abstract: Conditioned medium containing immune interferon (IFN) activity was prepared by stimulating spleen lymphocytes obtained from inbred SC chickens with 10 micrograms concanavalin A (Con A) for 48 h. Pretreatment of spleen cells with monoclonal antibody against CD4, but not CD8, abrogated IFN production suggesting that CD4+ lymphocytes are responsible for immune IFN production. Immune IFN was purified 25-fold from Con A conditioned medium using controlled-pore glass column chromatography resulting in an increase in specific antiviral activity from 7 to 3290 units mg-1. Partially purified immune IFN retained antiviral and macrophage-activating factor (MAF)-like activities. Normal peripheral blood macrophages, when cultured in the presence of partially purified immune IFN, showed a dose-dependent increase in cell surface major histocompatibility complex Class II antigen expression by flow cytometry. Northern blot analysis of mRNA obtained from IFN-treated macrophages showed a concomitant increase in Class II gene expression. This effect was more obvious in cells induced for 48 h than in those induced for 24 h. These results strongly suggest that existence of an avian homologue of the MAF-like activity.
authors.affiliation: USDA, Beltsville Agricultural Research Center, MD 20705, USA.
authors.forename: B
authors.initials: B
authors.lastname: Kaspers
authors.forename: H S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: M C
authors.initials: MC
authors.lastname: Jenkins
authors.forename: G T
authors.initials: GT
authors.lastname: Pharr
mesh_terms: D000818:Animals; D015152:Blotting, Northern / Q000662:veterinary; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens / Q000276:immunology; D003208:Concanavalin A; D017077:Culture Media, Conditioned; D004305:Dose-Response Relationship, Drug; D005802:Genes, MHC Class II; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D007372:Interferons / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000502:physiology; D016215:Macrophage-Activating Factors / Q000096:biosynthesis; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D013154:Spleen / Q000166:cytology
chemical_list: D017077:Culture Media, Conditioned; D000949:Histocompatibility Antigens Class II; D016215:Macrophage-Activating Factors; D012333:RNA, Messenger; D003208:Concanavalin A; D007372:Interferons