Steroidal glycosides from Allium macleanii and A. senescens, and their inhibitory activity on tumour promoter-induced phospholipid metabolism of HeLa cells.
A new polyhydroxylated cholestane trisdesmoside and a new spirostanol pentasaccharide, together with five known spirostanol saponins, were isolated from the bulbs of Allium macleanii, and two known spirostanol saponins were isolated from the bulbs of A. senescens. The identification and structural assignments of the steroidal glycosides were performed by spectroscopic analysis and hydrolysis. Furthermore, the isolated compounds were evaluated for inhibitory activity on 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated 32P-incorporation into phospholipids of HeLa cells, which is recognized as an excellent primary screening test to identify new antitumour-promoter compounds.
Inoue
T
T
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
Mimaki
Y
Y
Sashida
Y
Y
Nishino
A
A
Satomi
Y
Y
Nishino
H
H
D000490:Allium; D016588:Anticarcinogenic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002273:Carcinogens / Q000633:toxicity*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D010743:Phospholipids / Q000378:metabolism*; D012503:Saponins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity*
D016588:Anticarcinogenic Agents; D002273:Carcinogens; D009844:Oligosaccharides; D010743:Phospholipids; D012503:Saponins; D013755:Tetradecanoylphorbol Acetate