Platelet-derived growth factor (PDGF) and receptor (PDGFR) expression in human meningiomas: correlations with clinicopathological features and cytogenetic analysis.
PDGFs and their receptors expression were examined in a series of 46 meningiomas by using specific monoclonal antibodies. The immunostaining was quantified by an image analyser and the results correlated with clinical and morphological data (histological type and grade). In addition, since the PDGFB chain is encoded by the c-sis proto-oncogene localized on chromosome 22 and because monosomy 22 has been frequently reported in meningiomas, PDGFs and PDGFRs expression have been correlated with cytogenetic analysis performed in 29 cases. The results demonstrate PDGF A and PDGF B expression in most meningioma specimens and co-expression of these growth factors in numerous cells. PDGF A and B immunoreactivity was related to histological grade. PDGFR beta expression was strong in almost all meningiomas whereas PDGFR alpha was low. PDGFR alpha expression was related to tumour location and grade and PDGFR beta to histological subtype only. The cytogenetic analysis was not related to PDGFB chain expression. Taken together these data further confirm PDGF and PDGFR expression in human meningioma; PDGF may exist as an heterodimer (AB) as well as its receptor. The lack of correlation between cytogenetic analysis and PDGF values, the low level of PDGFB in recurrent meningiomas suggests that it is unlikely that the c-sis proto-oncogene plays an important role in the genesis of meningiomas.
Figarella-Branger
D
D
Department of Pathology and Neuropathology, Hôpital de la Timone, Marseille, France.
Vagner-Capodano
A M
AM
Bouillot
P
P
Graziani
N
N
Gambarelli
D
D
Devictor
B
B
Zattara-Cannoni
H
H
Bianco
N
N
Grisoli
F
F
Pellissier
J F
JF
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D005260:Female; D006801:Humans; D007158:Immunologic Techniques; D007536:Isomerism; D007621:Karyotyping; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008579:Meningioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008875:Middle Aged; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D017479:Receptors, Platelet-Derived Growth Factor / Q000378:metabolism*; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal; C000715470:MAS1 protein, human; D010982:Platelet-Derived Growth Factor; D000090063:Proto-Oncogene Mas; D017479:Receptors, Platelet-Derived Growth Factor