title: Generation of giant protoplasts of Escherichia coli and an inner-membrane anion selective conductance.
abstract: Established methods were modified and combined to generate unilamellar giant protoplasts in order to study the electric events on the cytoplasmic membrane of Escherichia coli with patch-clamp technique. The activities of many types of conductances were encountered, one of them is characterized here. This channel conductance is 109 pS in 150 mM KCl; it prefers anions, it is highly voltage dependent and is blocked by micromolar concentrations of anthracene-9-carboxylic acid.
authors.affiliation: Laboratory of Molecular Biology, University of Wisconsin, Madison 53706, USA.
authors.forename: A
authors.initials: A
authors.lastname: Kubalski
mesh_terms: D000838:Anions; D000873:Anthracenes / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology*; D018118:Chloride Channels / Q000037:antagonists & inhibitors; D004926:Escherichia coli / Q000502:physiology* / Q000648:ultrastructure; D017136:Ion Transport; D018408:Patch-Clamp Techniques; D011523:Protoplasts
chemical_list: D000838:Anions; D000873:Anthracenes; D018118:Chloride Channels; C010030:9-anthroic acid