The cerebral neurons of Helix aspersa during hibernation. Changes in the cytochemical detection of calmodulin, cytoskeletal components and phosphatases.
Some markers of the intracellular systems that regulate neuronal activity and morphology were analyzed in the cerebral ganglion of hibernating snails (Helix aspersa), in comparison with active animals. The immunocytochemical expression of a calcium-binding protein, i.e. calmodulin, and some cytoskeletal components, i.e. 200 kDa phosphorylated neurofilament protein (pNFH), microtubule associated protein 2 (MAP2) and alpha-tubulin were analyzed by the use of a panel of antibodies raised against mammal antigens. Moreover, by enzymatic reactions the Ca(2+)-ATPase and alkaline phosphatase (AIPase) activities were demonstrated. In comparison with the active phase, the hibernation induced an increase in the immunopositivity for calmodulin in all the neurons. The increase may be linked to unmasking of immunoreactive epitopes due to conformational changes of the protein, which in turn may be a consequence of a reduction or absence of binding with calcium ions or of a real increase in the amount of calmodulin in the somata of neurons. In any event, both the hypotheses indicate that neurons have decreased or suppressed the Ca(2+)-dependent mechanisms as also shown by the lower Ca(2+)-ATPase activity. Nevertheless, the AIPase activity, which was localized in the epineural sheat, was not significantly changed during hibernation and this supports that some metabolic activities are preserved in the hibernated animals. Changes in the immunopositivity for cytoskeletal components were found. There was an increase in the epitopes recognized by the mammalian pNF antibody, that concerned both the positivity of the entire cytoplasm of some clusters of metacerebral neurons and the intensity of the reaction. This would be aimed to improve the stability of the somata and primary neurites. Moreover, the decrease of alpha-tubulin and MAP2 immunopositivity, suggests that a disassembly of microtubules have occurred. The findings indicate that the transport of vesicles in the axons is slowed down during hibernation. In fact, research in progress show that the patterns of neurotransmission and neuromodulation are also deeply modified.
Vignola
C
C
Dipartimento di Biologia Animale, Università di Pavia, Italy.
Fenoglio
C
C
Scherini
E
E
Bernocchi
G
G
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000737:chemistry; D001923:Brain Chemistry / Q000502:physiology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002147:Calmodulin / Q000032:analysis*; D003599:Cytoskeleton / Q000737:chemistry*; D017952:Ganglia, Invertebrate / Q000737:chemistry*; D006372:Helix, Snails / Q000033:anatomy & histology / Q000737:chemistry*; D006605:Hibernation / Q000502:physiology; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D009474:Neurons / Q000737:chemistry / Q000201:enzymology; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D013194:Staining and Labeling
D015415:Biomarkers; D002147:Calmodulin; D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases; D000252:Calcium-Transporting ATPases