Generation of memory B cells and plasma cells in vitro.
After germinal center B cells undergo somatic mutation and antigen selection, they become either memory B cells or plasma cells, but the signal requirements that control entry into either pathway have been unclear. When purified human germinal center cells were cultured with interleukin-2, interleukin-10, and cells expressing CD40 ligand, cells with characteristics of memory B cells were generated. Removal of CD40 ligand from the system resulted in terminal differentiation of germinal center B cells into cells with the characteristics of plasma cells. These results indicate that CD40 ligand directs the differentiation of germinal center B cells toward memory B cells rather than toward plasma cells.
Arpin
C
C
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Déchanet
J
J
Van Kooten
C
C
Merville
P
P
Grouard
G
G
Brière
F
F
Banchereau
J
J
Liu
Y J
YJ
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD / Q000032:analysis; D018951:Antigens, CD20; D000943:Antigens, Differentiation / Q000032:analysis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D023201:CD40 Ligand; D002454:Cell Differentiation / Q000276:immunology*; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000032:analysis; D007156:Immunologic Memory / Q000276:immunology; D016130:Immunophenotyping; D008198:Lymph Nodes / Q000166:cytology; D008562:Membrane Glycoproteins / Q000276:immunology; D010950:Plasma Cells / Q000276:immunology*
D015703:Antigens, CD; D018951:Antigens, CD20; D000943:Antigens, Differentiation; D000944:Antigens, Differentiation, B-Lymphocyte; D007132:Immunoglobulin Isotypes; D008562:Membrane Glycoproteins; D023201:CD40 Ligand; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1