title: Functional characterization of the signal transduction events mediated by Fc epsilon RI alpha and gamma chimeric receptors.
abstract: Chimeric receptors containing the Fc epsilon RI alpha and gamma subunit domains were constructed, stably transfected into RBL-2H3 cells, and characterized for the biochemical events which are elicited upon receptor aggregation. Chimeric receptors containing the extracellular (EC) domain of the human Fc epsilon RI alpha subunit, or the EC domain of the p55 subunit of the interleukin-2 receptor were fused to the human Fc epsilon RI gamma subunit transmembrane and cytoplasmic (CT) domains or only the CT domain. The chimeras generated included alpha/gamma/gamma, I/gamma/gamma, alpha/I/gamma or I/I/gamma. The results indicate that both the Fc epsilon RI alpha EC domain and the Fc epsilon RI alpha CT domain are essential for signalling.
authors.affiliation: Department of Inflammation and Autoimmune Disease, Hoffmann-La Roche, Nutley, NJ 07110, USA.
authors.forename: B
authors.initials: B
authors.lastname: Repetto
authors.forename: G
authors.initials: G
authors.lastname: Bandara
authors.forename: G A
authors.initials: GA
authors.lastname: Wiggan
authors.forename: A M
authors.initials: AM
authors.lastname: Gilfillan
authors.forename: J P
authors.initials: JP
authors.lastname: Kochan
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D006636:Histamine Release / Q000502:physiology; D015471:Leukemia, Basophilic, Acute / Q000473:pathology; D051381:Rats; D011940:Receptor Aggregation; D017455:Receptors, IgE / Q000145:classification / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D017455:Receptors, IgE; D011993:Recombinant Fusion Proteins; D016718:Arachidonic Acid