Molecular study of the interferon genes in chronic myeloid leukemia.
The interferons alpha, beta, and w (IFNA, IFNB, IFNW), are a family of genes that have been mapped on the short arm of chromosome 9 (9p21-22). Deletions of genetic material on 9p are frequently observed in hematological diseases, particularly in lymphoid neoplasias. In this paper we have performed the molecular studies of IFNA and IFNB genes in chronic myeloid leukemia (CML) in order to determine if the deletions of these genes are prevalent in this pathology. Forty CML patients, Philadelphia positive or with BCR/ABL rearrangement, were studied at diagnosis. The analysis of IFNA and IFNB genes was performed by Southern and dot blot techniques. Homozygous or hemizygous deletions of IFNA and IFNB genes could not be detected, indicating that deletions of these genes would not be present or would be a very infrequent event in the chronic phase of the CML patients.
Larripa
I
I
Depto Genética, Instituto de Investigaciones Hematológicas de la Academia Nacional de Medicina de Buenos Aires, Argentina.
Giere
I
I
Slavutsky
I
I
Diaz
M
M
D000328:Adult; D000368:Aged; D015139:Blotting, Southern; D001853:Bone Marrow / Q000473:pathology; D002899:Chromosomes, Human, Pair 9; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D017353:Gene Deletion; D005796:Genes; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics*; D016899:Interferon-beta / Q000235:genetics*; D007372:Interferons / Q000235:genetics*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011990:Receptors, Transferrin / Q000235:genetics
D004273:DNA, Neoplasm; D016898:Interferon-alpha; D011990:Receptors, Transferrin; D016899:Interferon-beta; D007372:Interferons