title: Gliadin-specific T cell responses in peripheral blood of healthy individuals involve T cells restricted by the coeliac disease associated DQ2 heterodimer.
abstract: Coeliac disease (CD) is probably caused by an abnormal immune response towards wheat gliadin in the small intestine. We found that gliadin-specific T cells from the small intestinal mucosa of HLA-DQ2 positive CD patients were almost exclusively restricted by the disease-associated DQ2 molecule. In the peripheral blood of CD patients, a large proportion of gliadin-specific T cells were found to be restricted by DQ molecules, including DQ2, but many were instead restricted by DR or DP molecules of the patient. We have now investigated gliadin-specific T cell responses in peripheral blood from healthy individuals. Four of 20 persons tested had strong in vitro responses and were used as donors for gliadin-specific T cell clones. We found gliadin-specific T cells restricted by the CD-associated DQ2 molecule in peripheral blood for two of these four individuals. It is the presence of such T cells also in the small intestinal mucosa which seems typical of CD.
authors.affiliation: Institute of Transplantation Immunology, University of Oslo, Norway.
authors.forename: K
authors.initials: K
authors.lastname: Jensen
authors.forename: L M
authors.initials: LM
authors.lastname: Sollid
authors.forename: H
authors.initials: H
authors.lastname: Scott
authors.forename: G
authors.initials: G
authors.lastname: Paulsen
authors.forename: K
authors.initials: K
authors.lastname: Kett
authors.forename: E
authors.initials: E
authors.lastname: Thorsby
authors.forename: K E
authors.initials: KE
authors.lastname: Lundin
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D002446:Celiac Disease / Q000235:genetics* / Q000276:immunology; D002999:Clone Cells; D004848:Epithelium; D000939:Epitopes; D005903:Gliadin / Q000276:immunology*; D006683:HLA-DQ Antigens / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007421:Intestine, Small / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D006683:HLA-DQ Antigens; C053186:HLA-DQ2 antigen; D006684:HLA-DR Antigens; D005903:Gliadin