Modelling cortical cataractogenesis. 16. Leakage of lactate dehydrogenase: a new method for following cataract development in cultured lenses.
The in vitro effect of glucose on cultured rat lenses was correlated with (i) the time-dependent leakage of lactate dehydrogenase (LDH) into defined medium; (ii) the appearance of the lens under the dissection microscope; and (iii) the leakage of lens cytosolic proteins. A protective effect of 1 mM Vitamin C (VC) was also found: the extent of opacification, LDH and gamma-crystallin release were reduced if 1 mM VC was included in the medium. Using the above parameters provides a new and more rapid technique to follow lens opacification in vitro.
Kilic
F
F
Department of Biochemistry, Faculty of Medicine, University of Western Ontario, London, Canada.
Trevithick
J R
JR
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002386:Cataract / Q000201:enzymology* / Q000503:physiopathology* / Q000517:prevention & control; D016923:Cell Death; D002478:Cells, Cultured; D003459:Crystallins / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D005947:Glucose / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D007908:Lens, Crystalline / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar
D003459:Crystallins; D007770:L-Lactate Dehydrogenase; D005947:Glucose; D001205:Ascorbic Acid