Deleted in oral cancer-1 (doc-1), a novel oral tumor suppressor gene.
We have identified, isolated, and partially characterized doc-1, a novel cDNA sequence whose activity is consistent with a suppressor of hamster oral carcinogenesis. Doc-1 is an evolutionarily conserved gene exhibiting loss of heterozygosity and marked reduction in expression in malignant hamster oral keratinocytes. The full-length doc-1 cDNA encodes an 87 amino acid product that shows a significant homology to one of the seven novel genes induced in mouse fibroblasts by TNF-alpha. Transfection of the full-length doc-1 cDNA into malignant hamster oral keratinocytes alters the behavior of the recipients in terms of morphology, growth rate, and anchorage-independent growth, suggesting reversion of transformation phenotypes. We propose that doc-1 is a novel tumor suppressor gene in oral cancer development.
Todd
R
R
Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Boston 02114, USA.
McBride
J
J
Tsuji
T
T
Donoff
R B
RB
Nagai
M
M
Chou
M Y
MY
Chiang
T
T
Wong
D T
DT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D017353:Gene Deletion; D016147:Genes, Tumor Suppressor; D015603:Keratinocytes / Q000502:physiology; D008969:Molecular Sequence Data; D009062:Mouth Neoplasms / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
C096151:CDK2AP1 protein, human; D018076:DNA, Complementary; D011506:Proteins; D025521:Tumor Suppressor Proteins