Mutational analysis of the fic promoter recognized by RpoS (sigma 38) in Escherichia coli.
fic gene expression at the stationary phase is dependent on RpoS (sigma 38). For this paper, the fic promoter (P fic) mutants were isolated; C at the position -34 was changed into T. They did not show the stationary phase-specific expression dependent on RpoS in vivo. Furthermore, it was confirmed that they were recognized by E sigma 70 as efficiently as E sigma 38 in vitro.
Utsumi
R
R
Department of Agricultural Chemistry, Kinki University, Nara, Japan.
Nakayama
T
T
Iwamoto
N
N
Kohda
K
K
Kawamukai
M
M
Tanabe
H
H
Tanaka
K
K
Takahashi
H
H
Noda
M
M
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000378:metabolism*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria