Identification of the domain which determines the g,m serotype of the flagellin of Salmonella enteritidis.
Clones expressing fragments of the flagellin protein of Salmonella enteritidis were constructed and screened with a g,m-specific monoclonal antibody. Results showed that the g,m epitope is localized between amino acids 258 and 348 of the flagellin. The fliC gene, encoding the flagellin of S. enteritidis, was proven to be the only flagellin gene present in S. enteritidis.
van Asten
A J
AJ
Department of Bacteriology, University of Utrecht, The Netherlands.
Zwaagstra
K A
KA
Baay
M F
MF
Kusters
J G
JG
Huis in't Veld
J H
JH
van der Zeijst
B A
BA
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D003001:Cloning, Molecular; D018604:Epitope Mapping; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005408:Flagellin / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D012477:Salmonella enteritidis / Q000235:genetics / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D012703:Serotyping
D000942:Antigens, Bacterial; D000939:Epitopes; D010446:Peptide Fragments; D005408:Flagellin