title: Identification of a graft versus host disease-associated human minor histocompatibility antigen.
abstract: Minor histocompatibility antigen disparities between human leukocyte antigen (HLA)-matched bone marrow donors and recipients are a major risk factor for graft versus host disease (GVHD). An HLA-A2.1-restricted cytotoxic T cell clone that recognized the minor histocompatibility antigen HA-2 was previously isolated from a patient with severe GVHD after HLA-identical bone marrow transplantation. The HLA-A2.1-bound peptide representing HA-2 has now been identified. This peptide appears to originate from a member of the non-filament-forming class I myosin family. Because HA-2 has a phenotype frequency of 95 percent in the HLA-A2.1-positive population, it is a candidate for immunotherapeutic intervention in bone marrow transplantation.
authors.affiliation: Department of Immunohaematology, University Hospital, Leiden, Netherlands.
authors.forename: J M
authors.initials: JM
authors.lastname: den Haan
authors.forename: N E
authors.initials: NE
authors.lastname: Sherman
authors.forename: E
authors.initials: E
authors.lastname: Blokland
authors.forename: E
authors.initials: E
authors.lastname: Huczko
authors.forename: F
authors.initials: F
authors.lastname: Koning
authors.forename: J W
authors.initials: JW
authors.lastname: Drijfhout
authors.forename: J
authors.initials: J
authors.lastname: Skipper
authors.forename: J
authors.initials: J
authors.lastname: Shabanowitz
authors.forename: D F
authors.initials: DF
authors.lastname: Hunt
authors.forename: V H
authors.initials: VH
authors.lastname: Engelhard
mesh_terms: D000595:Amino Acid Sequence; D016026:Bone Marrow Transplantation; D000939:Epitopes; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology*; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D013058:Mass Spectrometry; D015778:Minor Histocompatibility Antigens / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry / Q000276:immunology*; D009842:Oligopeptides / Q000737:chemistry / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D000939:Epitopes; C080145:HA-2 antigen; D015789:HLA-A2 Antigen; D015778:Minor Histocompatibility Antigens; D009363:Neoplasm Proteins; D009842:Oligopeptides