title: Interplay between the human TCR/CD3 epsilon and the B-cell antigen receptor associated Ig-beta (B29).
abstract: T and B lymphocytes are characterized by the surface expression of highly variable antigen receptors called the T-cell (TCR) and B-cell (BCR) receptor. In both B and T cells, binding of antigen to their respective surface receptors results in transmembrane signaling which leads either to programmed cell death or to proliferation and differentiation. The human and murine TCR consist of the highly variable TCR alpha and beta or gamma and delta chains recognizing antigen and the non-covalently associated invariable CD3 complex (gamma, delta, epsilon) and zeta. The antigen-recognizing surface membrane-bound immunoglobulin (Ig) molecules on the surface of human and murine B cells are non-covalently associated with a heterodimeric protein complex of Ig-alpha (MB-1) and Ig-beta (B29). Like the CD3 complex associated with TCR the Ig-associated proteins are predicted to regulate the assembly and transport of the Ig complex to the cell surface and to couple membrane-bound Ig to intracellular signal transduction pathways. To gain more insight into structure/function relationships between CD3 proteins and Ig-alpha and Ig-beta, we transiently co-transfected pairs of expression vectors encoding either TCR/CD3 chains on the one hand and Ig-alpha or Ig-beta on the other into Cos cells. Thus we found a very strong interaction between CD3 epsilon and Ig-beta mediated by the extracellular domains. Experiments in which we could stain Jurkat T cells with soluble Ig-beta but not Ig-alpha protein indicated the recognition of CD3 epsilon by Ig-beta even in the context of the whole TCR/CD3 complex.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Division of Immunology, Beth Israel Hospital, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: B
authors.initials: B
authors.lastname: Müller
authors.forename: L
authors.initials: L
authors.lastname: Cooper
authors.forename: C
authors.initials: C
authors.lastname: Terhorst
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D017252:CD3 Complex / Q000235:genetics / Q000378:metabolism*; D002450:Cell Communication; D002478:Cells, Cultured; D005434:Flow Cytometry; D005822:Genetic Vectors; D006801:Humans; D018448:Models, Immunological; D011233:Precipitin Tests; D011485:Protein Binding; D011947:Receptors, Antigen, B-Cell / Q000235:genetics / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015398:Signal Transduction; D013194:Staining and Labeling; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000166:cytology; D014162:Transfection
chemical_list: D017252:CD3 Complex; D011947:Receptors, Antigen, B-Cell; D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins