Antioxidant properties of demethyldiisoeugenol.
The antioxidant properties of demethyldiisoeugenol were investigated in this study using various models. Demethyldiisoeugenol inhibited Fe(2+)-induced lipid peroxidation in rat brain homogenate in a concentration-dependent manner with an IC50 1.8 +/- 0.1 microM. Demethyldiisoeugenol was more effective than alpha-tocopherol and BHT in reducing the stable free radical diphenyl-2-picrylhydrazyl (DPPH). It also scavenged superoxide anion generated by xanthine/xanthine oxidase and peroxyl radical (ROO.) derived from 2,2'-azobis(2-amidinopropane)dihydrochloride (AAPH) in aqueous system with a stoichiometric factor of 1.3 +/- 0.2. Furthermore, it prevented conjugated-diene formation and apolipoprotein B (apo B) oxidation of LDL. However, demethyldiisoeugenol did not scavenge 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN)-derived peroxyl radical in hexane. It also did not chelate Fe2+, did not inhibit xanthine oxidase activity or possessed hydroxyl radical (.OH) scavenging activity. Experimental results indicate that demethyldiisoeugenol is a potentially effective antioxidant and can protect rat brain homogenate and LDL against oxidation.
Ko
F N
FN
Pharmacological Institute, College of Medicine, Taipei, Taiwan.
Liao
C H
CH
Kuo
Y H
YH
Lin
Y L
YL
D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D001055:Apolipoproteins B / Q000737:chemistry; D001921:Brain / Q000378:metabolism; D002396:Catechols / Q000737:chemistry*; D002474:Cell-Free System; D003300:Copper / Q000737:chemistry; D003855:Deoxyribose / Q000737:chemistry; D005054:Eugenol / Q000031:analogs & derivatives* / Q000737:chemistry; D005231:Fatty Acids, Unsaturated / Q000737:chemistry; D005609:Free Radicals; D066298:In Vitro Techniques; D007192:Indenes / Q000737:chemistry*; D007502:Iron Chelating Agents / Q000737:chemistry; D008054:Lipid Peroxides / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats
D000975:Antioxidants; D001055:Apolipoproteins B; D002396:Catechols; D005231:Fatty Acids, Unsaturated; D005609:Free Radicals; D007192:Indenes; D007502:Iron Chelating Agents; D008054:Lipid Peroxides; D008077:Lipoproteins, LDL; C095957:demethyldiisoeugenol; D005054:Eugenol; C087580:diisoeugenol; D003855:Deoxyribose; D003300:Copper; C013641:parinaric acid