Acidic and basic fibroblast growth factors enhance neurite outgrowth in cultured rat spinal cord neurons.
We have studied neurotrophic effects of acidic fibroblast growth factor (aFGF) and basic fibroblast growth factor (bFGF) on explanted ventral and dorsal spinal cord cultures from 13- and 14-day-old rat embryos. Cultures treated with aFGF and bFGF significantly enhanced neurite outgrowth with cultures of ventral spinal cord, but not with cultures of dorsal spinal cord. Our data suggest that aFGF and bFGF are potent neurotrophic factors on rat ventral spinal cord neurons in vitro.
Iwasaki
Y
Y
Fourth Department of Internal Medicine, Toho University Ohashi Hospital, Tokyo, Japan.
Shiojima
T
T
Ikeda
K
K
Tagaya
N
N
Kobayashi
T
T
Kinoshita
M
M
D000818:Animals; D002478:Cells, Cultured; D005314:Embryonic and Fetal Development / Q000187:drug effects; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D016501:Neurites / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000187:drug effects* / Q000196:embryology / Q000648:ultrastructure
D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1