Automated extraction of acetylgestagens from kidney fat by matrix solid phase dispersion.
A new extraction method for the acetylgestagens medroxyprogesterone acetate (MPA), chloromadinone acetate and megestrol acetate, from kidney fat, has been developed. The method is a combination of matrix solid phase dispersion and solid phase extraction and is simpler and safer than previous methods, especially as it can be automated. The recovery was estimated as 59 +/- 5% (mean +/- standard deviation) for MPA. For screening purposes detection can be achieved using a commercially available enzyme immunoassay kit giving detection limits in the range of 1.0-2.0 ng g-1.
Rosén
J
J
National Food Administration, Uppsala, Sweden.
Hellenäs
K E
KE
Törnqvist
P
P
Shearan
P
P
D000003:Abattoirs; D000273:Adipose Tissue / Q000737:chemistry*; D001331:Automation / Q000379:methods; D002715:Chlormadinone Acetate / Q000032:analysis / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007124:Immunoenzyme Techniques; D007668:Kidney; D017258:Medroxyprogesterone Acetate / Q000032:analysis / Q000302:isolation & purification*; D008535:Megestrol / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D019290:Megestrol Acetate; D011865:Radioisotope Dilution Technique; D011933:Reagent Kits, Diagnostic; D014316:Tritium
D011933:Reagent Kits, Diagnostic; D002715:Chlormadinone Acetate; D014316:Tritium; D017258:Medroxyprogesterone Acetate; D008535:Megestrol; D019290:Megestrol Acetate