Liquid storage of peripheral blood progenitor cells for transplantation.
Peripheral blood progenitor cells (PBPC) were mobilised by recombinant human G-CSF (rhG-CSF) and cyclophosphamide, harvested by apheresis from 9 patients with NHL and stored at 4 degrees C without further manipulation. They were then reinfused after high-dose chemotherapy. We monitored the change in colony-forming units-granulocyte, macrophage (CFU-GM) proliferation as well as plasma glucose, lactate and pH levels over the period of the study. In an attempt to maintain CFU-GM numbers, rhG-CSF was added to storage bags at collection and 48 h later. Our observations suggest that cell viability is well maintained and that CFU-GM numbers rise over the first 48 h of storage before falling rapidly. Metabolic changes cause a fall in the pH and glucose levels with a reciprocal rise in plasma lactate. The addition of rhG-CSF at a concentration of 10 ng/ml to cells in storage showed no detectable benefit. Following storage for 96 h, 77% (SEM +/- 8%) of the initial CFU-GM remained.
Jestice
H K
HK
Department of Clinical Haematology, Addenbrooke's NHS Trust, Cambridge, UK.
Scott
M A
MA
Ager
S
S
Tolliday
B H
BH
Marcus
R E
RE
D001781:Blood Component Removal; D002470:Cell Survival; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D014021:Tissue Preservation / Q000379:methods
D016179:Granulocyte Colony-Stimulating Factor