title: Culture of human adult endothelial cells on endarterectomy surfaces.
abstract: OBJECTIVE
  Endothelial cell seeding of prosthetic grafts has not been as successful as initially hoped and the application of seeding technology to alternative reconstructive procedures such as endarterectomy and angioplasty has been increasingly considered. The success of such seeding depends on the ability of the seeded cells to attach to, and form a monolayer on the endarterectomised vessel wall which was the aim of this study.
  
  
  METHODS
  Using a seeding chamber model, heterologous human adult endothelial cells were seeded onto fresh human endarterectomy specimens and cultured. Studies of endothelial call adherence to endarterectomy specimens were performed using 111-Indium oxine labelled cells using methodology analogous to graft seeding.
  
  
  RESULTS
  Mean endothelial cell adherence of 70% (S.D. 10%) after 1 h incubation was achieved and the successful development of a monolayer of human adult venous endothelial cells on endarterectomised arteries was demonstrated in vitro.
  
  
  CONCLUSIONS
  These results indicate that closed endarterectomy appears to offer a surface with cell attachment that is superior to prosthetic grafts. Where femoral endarterectomy is appropriate, endothelial seeding potentially offers a method of reducing thrombogenicity and intimal hyperplasia, improving patency and avoiding a prosthetic graft whilst preserving collateral circulation and autologous vein.
authors.affiliation: Department of Vascular Surgery, Manchester Royal Infirmary and Medical School, U.K.
authors.forename: J V
authors.initials: JV
authors.lastname: Smyth
authors.forename: O B
authors.initials: OB
authors.lastname: Rooney
authors.forename: P D
authors.initials: PD
authors.lastname: Dodd
authors.forename: M G
authors.initials: MG
authors.lastname: Walker
mesh_terms: D000328:Adult; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002478:Cells, Cultured; D004691:Endarterectomy; D004730:Endothelium, Vascular / Q000166:cytology*; D005263:Femoral Artery / Q000166:cytology / Q000601:surgery; D006801:Humans; D008855:Microscopy, Electron, Scanning; D012501:Saphenous Vein / Q000166:cytology; D013499:Surface Properties; D013997:Time Factors