title: Chronic social stress increases levels of preprogalanin mRNA in the rat locus coeruleus.
abstract: Galanin is a 29 amino acid neuropeptide that coexists with norepinephrine in approximately 80% of locus coeruleus (LC) neurons in the rat. The effects of chronic, naturalistic stress on preprogalanin mRNA in the LC was studied. The visible burrow system (VBS) served as the stress paradigm. Long-Evans rats (three males and two females) were housed together in the VBS for 13 days. The males established dominance hierarchies during this period. On day 14, preprogalanin mRNA in the LC was significantly elevated in subordinate rats compared to dominant and control rats, as measured by quantitative in situ hybridization. Levels of mRNA were positively correlated with the number of wounds by day 7 and day 14 and negatively correlated with body weight gain by day 14. These results suggest that the neuropeptide galanin may be overexpressed during chronic stress in rats.
authors.affiliation: Section on Behavioral Neuropharmacology, National Institute of Mental Health, Bethesda, MD 20892, USA.
authors.forename: P V
authors.initials: PV
authors.lastname: Holmes
authors.forename: D C
authors.initials: DC
authors.lastname: Blanchard
authors.forename: R J
authors.initials: RJ
authors.lastname: Blanchard
authors.forename: L S
authors.initials: LS
authors.lastname: Brady
authors.forename: J N
authors.initials: JN
authors.lastname: Crawley
mesh_terms: D000818:Animals; D005260:Female; D019004:Galanin; D017403:In Situ Hybridization; D008125:Locus Coeruleus / Q000378:metabolism*; D008297:Male; D009479:Neuropeptides / Q000235:genetics; D010455:Peptides / Q000235:genetics; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D012919:Social Behavior; D013315:Stress, Psychological / Q000378:metabolism*; D013997:Time Factors
chemical_list: D009479:Neuropeptides; D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; D019004:Galanin