title: The role of fs(1)K10 in the localization of the mRNA of the TGF alpha homolog gurken within the Drosophila oocyte.
abstract: A critical step in Drosophila dorsoventral patterning is the movement of gurken mRNA from the anterior cortex of the oocyte to the oocyte's anterodorsal corner at stage 8 of oogenesis. Such movement is dependent on fs(1)K10. It has been proposed that fs(1)K10 mediates gurken mRNA movement by down-regulating gurken mRNA levels, thus ensuring that gurken mRNA does not saturate its receptors located in the oocyte's anterodorsal corner. In contradiction to this model, we show here--both genetically and immunocytochemically--that GRK protein levels are lower in the anterodorsal region of fs(1)K10 mutant oocytes than in the anterodorsal region of fs(1)K10+ oocytes. From this and other data, we propose a more direct role for fs(1)K10 in the gurken mRNA localization process.
authors.affiliation: Department of Biochemistry, University of Kansas, Lawrence 66045, USA.
authors.forename: T L
authors.initials: TL
authors.lastname: Serano
authors.forename: M
authors.initials: M
authors.lastname: Karlin-McGinness
authors.forename: R S
authors.initials: RS
authors.lastname: Cohen
mesh_terms: D000818:Animals; D016764:Cell Polarity; D015536:Down-Regulation; D004330:Drosophila / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D029721:Drosophila Proteins; D005260:Female; D017344:Genes, Insect; D007301:Insect Hormones / Q000235:genetics*; D008297:Male; D009154:Mutation; D009865:Oocytes / Q000378:metabolism*; D009866:Oogenesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016211:Transforming Growth Factor alpha / Q000235:genetics; D015291:Transforming Growth Factors / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D007301:Insect Hormones; D012333:RNA, Messenger; D016211:Transforming Growth Factor alpha; C083730:grk protein, Drosophila; D015291:Transforming Growth Factors