A sensitive novel staining agent for the resolved proteins on PAGE.
A more sensitive, stable and fast-reacting dye was isolated from the bark of Ratanjot by a simple solvent extraction procedure at high pH. The dye produced sharp, clear distinct stable bands of known proteins when resolved on polyacrylamide gel electrophoresis (PAGE) using sodium dodesyl sulfate (SDS), showing a rapid and strong affinity with protein molecules. Not only did proteins from peanut, separately stained with the new dye and Coomassie Brilliant Blue R-250, produce rapid comparable results, but also the protein bands were easily visualized and were less diffusible with clearer boundaries in the case of the new staining agent.
Ali
R
R
Department of Food Science and Technology, University of Karachi, Pakistan.
Sayeed
S A
SA
Khan
A A
AA
D004396:Coloring Agents; D004591:Electrophoresis, Polyacrylamide Gel; D010944:Plants / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods*
D004396:Coloring Agents; D011506:Proteins; D012394:Rosaniline Dyes; C048139:Coomassie blue