A macrophage receptor for oxidized low density lipoprotein distinct from the receptor for acetyl low density lipoprotein: partial purification and role in recognition of oxidatively damaged cells.
The binding and uptake of oxidatively modified low density lipoprotein (OxLDL) by mouse peritoneal macrophages occurs, in part, via the well characterized acetyl LDL receptor. However, several lines of evidence indicate that as much as 30-70% of the uptake can occur via a distinct receptor that recognizes OxLDL with a higher affinity than it recognizes acetyl LDL. We describe the partial purification and characterization of a 94- to 97-kDa plasma membrane protein from mouse peritoneal macrophages that specifically binds OxLDL. This receptor is shown to be distinct from the acetyl LDL receptor as well as from two other macrophage proteins that also bind OxLDL--the Fc gamma RII receptor and CD36. We suggest that this OxLDL-binding membrane protein participates in uptake of OxLDL by murine macrophages and also represents a receptor responsible for macrophage binding and phagocytosis of oxidatively damaged cells.
Ottnad
E
E
Department of Medicine, University of California, San Diego, La Jolla 92093-0682.
Parthasarathy
S
S
Sambrano
G R
GR
Ramprasad
M P
MP
Quehenberger
O
O
Kondratenko
N
N
Green
S
S
Steinberg
D
D
D000818:Animals; D015703:Antigens, CD / Q000737:chemistry; D018955:CD36 Antigens; D015815:Cell Adhesion Molecules; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism*; D008264:Macrophages / Q000737:chemistry / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism; D051379:Mice; D010084:Oxidation-Reduction; D011817:Rabbits; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000302:isolation & purification*; D011973:Receptors, LDL / Q000378:metabolism*; D051116:Receptors, Scavenger
D015703:Antigens, CD; D018955:CD36 Antigens; D015815:Cell Adhesion Molecules; D008077:Lipoproteins, LDL; D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D011973:Receptors, LDL; D051116:Receptors, Scavenger