title: Detection of a LFA-1-like epitope on the surface of erythrocytes infected with a strain of Plasmodium falciparum.
abstract: The adhesion of erythrocytes infected with Plasmodium falciparum (P. falciparum) is one of the major pathological features of severe malaria. Several potential receptors to endothelium for falciparum-infected erythrocyte on endothelium have been described. Recently, the malaria binding site on ICAM-1(CD54) has been mapped to a site distinct but overlapping with the LFA-1 (CD11a/CD18) site. We detected by flow cytometry, confocal laser microscopy and immunoprecipitation, a molecule expressed at the surface of erythrocytes infected with mature stages of the M96 strain of P. falciparum that was recognized by a monoclonal antibody (mAb) (TS1/22) directed against an LFA-1 epitope. However, this molecule was not recognized by mAbs directed against other epitopes of LFA-1 or against other integrins. Furthermore, the mAb TS1/22 partially inhibited cytoadherence of parasitized red blood cells to human-brain microvascular endothelial cells. The expression of a molecule sharing an epitope with human LFA-1 integrin on the parasitized erythrocyte surface could be involved in the sequestration of these cells and thus in the pathogenesis of severe disease.
authors.affiliation: Department of Pathology, University of Geneva, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Tacchini-Cottier
authors.forename: J N
authors.initials: JN
authors.lastname: Lou
authors.forename: D J
authors.initials: DJ
authors.lastname: Roberts
authors.forename: A M
authors.initials: AM
authors.lastname: Garcia
authors.forename: G E
authors.initials: GE
authors.lastname: Grau
mesh_terms: D000953:Antigens, Protozoan / Q000032:analysis*; D002448:Cell Adhesion; D004730:Endothelium, Vascular / Q000276:immunology*; D000939:Epitopes / Q000032:analysis*; D004910:Erythrocyte Membrane / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000276:immunology*; D016778:Malaria, Falciparum / Q000276:immunology*; D018613:Microscopy, Confocal; D011233:Precipitin Tests; D013997:Time Factors
chemical_list: D000953:Antigens, Protozoan; D000939:Epitopes; D016169:Lymphocyte Function-Associated Antigen-1