Determination of the enantiomers of metoprolol and its major acidic metabolite in human urine by high-performance liquid chromatography with fluorescence detection.
Enantiomers of metoprolol and its acidic metabolite H 117/04 were determined in human urine by high-performance liquid chromatography (HPLC) with fluorometric detection after chiral derivatization. The carboxyl functional group of the major metabolite was blocked by esterification after solid-phase extraction, which helped to quantitate this compound from interfering substances. The assay method was validated. The recovery of (-)- and (+)-metoprolol from urine was 86.3-90.5%; and the recovery of the (-)- and (+)-acidic metabolite H 117/04 from urine was 74.4-83.9% at different concentrations.
Li
F
F
Institut National de la Recherche Scientifique, INRS-Santé, Université du Québec, Pointe-Claire, Canada.
Cooper
S F
SF
Côté
M
M
D000328:Adult; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004951:Esterification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008790:Metoprolol / Q000737:chemistry / Q000652:urine*; D008875:Middle Aged; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism
D008790:Metoprolol