Accumulation of fibronectin in articular cartilage explants cultured with TGF beta 1 and fucoidan.
Fibronectin is a glycoprotein involved in cell matrix interactions. In osteoarthritis, fibronectin levels in the lesion cartilage are elevated up to 20-fold above control levels. In these experiments, explants of disease-free cartilage cultured in the presence of a combination of TGF beta 1 and the sulfated fucopolysaccharide, fucoidan, accumulated fibronectin at levels comparable to those found in osteoarthritic lesions. TGF beta 1 increased fibronectin synthesis, most of which was released to the medium. The addition of fucoidan favored retention of the newly synthesized fibronectin within the matrix. The fibronectin which accumulated as a result of these treatments was similar to the fibronectin in normal and osteoarthritic cartilage with respect to the ED-B+ alternative splice form. No change in the proteoglycan content of the cartilage explants with elevated fibronectin levels was detected.
Burton-Wurster
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James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853.
Zhang
D W
DW
Lust
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G
D000818:Animals; D002358:Cartilage, Articular / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004285:Dogs; D005353:Fibronectins / Q000096:biosynthesis*; D006025:Glycosaminoglycans / Q000494:pharmacology; D006618:Hip Dislocation, Congenital / Q000378:metabolism; D066298:In Vitro Techniques; D010003:Osteoarthritis / Q000378:metabolism*; D010426:Pentosan Sulfuric Polyester / Q000494:pharmacology; D011134:Polysaccharides / Q000494:pharmacology*; D011509:Proteoglycans / Q000032:analysis; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D005353:Fibronectins; D006025:Glycosaminoglycans; D011134:Polysaccharides; D011509:Proteoglycans; D016212:Transforming Growth Factor beta; C013786:A73025; D010426:Pentosan Sulfuric Polyester; C007789:fucoidan