Protein kinase C is not necessary for beta-casein gene induction by prolactin in HC11 mouse mammary cells.
HC11 mouse mammary cells cultured in the presence of insulin, cortisol and prolactin for 24 h accumulated beta-casein mRNA. When the specific inhibitor of protein kinase C, GF 109203 X, was added to the medium with the hormones, the accumulation of beta-casein mRNA was unaltered, although the protein kinase C activity was almost completely suppressed. This suggests that protein kinase C is not strictly necessary for prolactin to induce milk protein gene expression.
Mitev
V
V
INRA, unité de différenciation cellulaire, Jouy-en-Josas, France.
Lemnaouar
M
M
Puissant
C
C
Houdebine
L M
LM
D000818:Animals; D002364:Caseins / Q000235:genetics*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation; D007211:Indoles / Q000494:pharmacology; D008301:Maleimides / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D011388:Prolactin / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D015533:Transcriptional Activation
D002364:Caseins; D004791:Enzyme Inhibitors; D007211:Indoles; D008301:Maleimides; D012333:RNA, Messenger; D011388:Prolactin; D011493:Protein Kinase C; C070515:bisindolylmaleimide I