title: Lack of augmenting effect of interferon-gamma on dengue virus multiplication in human peripheral blood monocytes.
abstract: The effect of interferon-gamma (IFN-gamma) on dengue virus multiplication in human peripheral blood monocytes was investigated. Enriched monocytes were treated with IFN-gamma and then infected with dengue virus type 2 either directly or in the presence of optimal infection-enhancing levels of antibodies. Pretreatment of monocytes from dengue-immune donors with 100 IU/ml of IFN-gamma caused 12- to 97-fold and 13- to 137-fold reduction of virus yields at 24 hr after infection in the absence and presence of an anti-flavivirus monoclonal antibody, respectively. IFN-gamma also diminished virus yields when infection of monocytes from a donor who lacked anti-dengue antibody was enhanced 40-fold. The percentage of infected monocytes in IFN-gamma-pretreated cultures was similarly reduced. Dominance of the antiviral effect of IFN-gamma in monocytes is in contrast to an augmenting effect previously observed in the promonocytic cell line U937.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Chiang Mai University, Thailand.
authors.forename: N
authors.initials: N
authors.lastname: Sittisombut
authors.forename: N
authors.initials: N
authors.lastname: Maneekarn
authors.forename: A
authors.initials: A
authors.lastname: Kanjanahaluethai
authors.forename: W
authors.initials: W
authors.lastname: Kasinrerk
authors.forename: K
authors.initials: K
authors.lastname: Viputtikul
authors.forename: J
authors.initials: J
authors.lastname: Supawadee
mesh_terms: D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D003716:Dengue Virus / Q000276:immunology / Q000502:physiology*; D005416:Flavivirus / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology*; D018950:Lipopolysaccharide Receptors; D009000:Monocytes / Q000187:drug effects / Q000821:virology*; D017452:Receptors, IgG / Q000032:analysis; D011994:Recombinant Proteins; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D007106:Immune Sera; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG; D011994:Recombinant Proteins; D007371:Interferon-gamma