Monitoring anti-HPA-1a platelet antibody levels during pregnancy using the MAIPA test.
Anti-HPA-1a platelet antibody levels in pregnant women with a history of fetomaternal alloimmune thrombocytopenia (FMAIT) were monitored longitudinally using the monoclonal antibody immobilisation of platelet antigens (MAIPA) assay, in order to examine any variation in optical density (OD) readings obtained over the course of pregnancy and after delivery. Seven women were selected; 4 were studied retrospectively and 3 prospectively (the latter being treated with intravenous gammaglobulin; IVGG). Levels of anti-HPA-1a were measured at various intervals after delivery of the first affected infant, to post delivery of the following affected infant. A decrease in MAIPA OD was demonstrated in all patients during the course of these pregnancies. This assay is a useful tool for monitoring anti-HPA-1a in women with a history of infants affected with FMAIT. A maternal antibody 'resting' level prior to, or early in the first trimester, must be established for comparison.
Dawkins
B
B
Red Cross Blood Transfusion Service, Brisbane, Australia.
D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D016824:Antigens, Human Platelet / Q000235:genetics / Q000276:immunology*; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D006720:Homozygote; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007231:Infant, Newborn; D007518:Isoantibodies / Q000097:blood*; D008297:Male; D008991:Monitoring, Physiologic; D010976:Platelet Count; D011247:Pregnancy; D011446:Prospective Studies; D012189:Retrospective Studies; D005719:gamma-Globulins / Q000627:therapeutic use
D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; D007518:Isoantibodies; D005719:gamma-Globulins