Mechanisms involved in the uptake of D-glucose into the milk producing cells of rat mammary tissue.
There is a rapid stimulation of the rate of efflux of 3-O-methyl D-glucose from intact lactating rat mammary tissue slices in response to a change in the Na(+)-gradient across the basolateral plasma membrane. RNA extracted from lactating mammary glands contains a 4 kb transcript which hybridises with the cDNA for rabbit intestinal SGLT1. A fraction of the mammary gland cells which is enriched in endoplasmic, Golgi and plasma membranes contains a protein that is immunologically similar to the Na(+)-dependent D-glucose symporter present in the plasma membrane of polarised epithelial cells, e.g., rabbit enterocytes, ovine enterocytes and parotid acinar cells.
Shennan
D B
DB
Hannah Research Institute, Ayr, Scotland, U.K.
Beechey
R B
RB
D019325:3-O-Methylglucose; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000031:analogs & derivatives / Q000378:metabolism*; D066298:In Vitro Techniques; D007774:Lactation / Q000502:physiology*; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008562:Membrane Glycoproteins; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008757:Methylglucosides / Q000378:metabolism; D009004:Monosaccharide Transport Proteins; D012313:RNA / Q000235:genetics; D051381:Rats; D012964:Sodium / Q000378:metabolism; D051269:Sodium-Glucose Transporter 1
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D008757:Methylglucosides; D009004:Monosaccharide Transport Proteins; C495676:Slc5a1 protein, rat; D051269:Sodium-Glucose Transporter 1; D019325:3-O-Methylglucose; D012313:RNA; D012964:Sodium; D005947:Glucose