title: Rapid detection and biovar differentiation of Ureaplasma urealyticum in clinical specimens by PCR.
abstract: On the basis of the nucleotide sequence of the multiple-banded (MB) antigen genes of Ureaplasma urealyticum, a polymerase chain reaction (PCR) technique was developed for rapid detection and biovar differentiation of U. urealyticum in a total of 100 urogenital specimens from 50 female patients. Positive PCR UM-1 amplification was found in 28 cervical swabs and 31 urine samples. Overall agreement between PCR and culture was 95%. Members of the two biovars of U. urealyticum could be distinguished by the size of the PCR UM-1 amplification products. Biovar differentiation was also demonstrated by two additional sets of PCRs: PCR UM-2 and UM-3. The PCR UM-2 was used to amplify biovar 1, while PCR UM-3 amplified biovar 2 specifically. The results indicated that use of the MB antigen gene as a target for PCR amplification could provide rapid and specific detection and biotyping of ureaplasma DNA in urogenital samples.
authors.affiliation: Department of Laboratory Medicine, National Taiwan University Hospital, Taipei, ROC.
authors.forename: L J
authors.initials: LJ
authors.lastname: Teng
authors.forename: S W
authors.initials: SW
authors.lastname: Ho
authors.forename: H N
authors.initials: HN
authors.lastname: Ho
authors.forename: S J
authors.initials: SJ
authors.lastname: Liaw
authors.forename: H C
authors.initials: HC
authors.lastname: Lai
authors.forename: K T
authors.initials: KT
authors.lastname: Luh
mesh_terms: D015373:Bacterial Typing Techniques; D001483:Base Sequence; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016990:Ureaplasma urealyticum / Q000145:classification / Q000302:isolation & purification*