In vivo effects of acetaminophen on rat RBC and role of vitamin E.
Non-therapeutic toxic dose (250 mg/kg) of acetaminophen (paracetamol), in vivo to albino rats significantly decreased red cell reduced glutathione (GSH) content and activity of (Na+, K+)-ATPase enzyme, whereas osmotic fragility (O.F.) was increased. However, no change was observed in the activity of glutathione reductase (GR) after acetaminophen treatment, while acetaminophen plus vitamin E treated rats showed significant increase in GR activity. Supplementation of vitamin E to the drug treated rats effectively brought the GSH content, (Na+, K+)-ATPase activity and O.F. back to almost normal. The results suggest that acetaminophen toxic dose treatment induces metabolic and membranal alterations making red cells prone to hemolysis, while vitamin E which is an antioxidant shows its ameliorating role to these changes.
Suhail
M
M
Department of Biochemistry, Faculty of Science, University of Allahabad, India.
Ahmad
I
I
D000082:Acetaminophen / Q000633:toxicity*; D018712:Analgesics, Non-Narcotic / Q000633:toxicity*; D000818:Animals; D004912:Erythrocytes / Q000187:drug effects*; D008297:Male; D051381:Rats; D014810:Vitamin E / Q000494:pharmacology*
D018712:Analgesics, Non-Narcotic; D014810:Vitamin E; D000082:Acetaminophen