Efficient elution of purified proteins from polyvinylidene difluoride membranes (Immobilon) after transfer from SDS-PAGE and their use as immunogens.
Following separation of proteins by SDS-PAGE, they are electroblotted onto polyvinylidene difluoride membranes (Immobilon). Protein bands of interest are excised, and the proteins are eluted from the membrane with detergent-containing buffers at pH 9.5. The method routinely yields recovery of 70-90%, and this is independent of protein molecular weight.
Szewczyk
B
B
Department of Microbiology and Molecular Genetics, University of California, College of Medicine, Irvine.
Summers
D F
DF
D000941:Antigens / Q000276:immunology / Q000302:isolation & purification*; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D008567:Membranes, Artificial; D011145:Polyvinyls; D011489:Protein Denaturation; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling; D005465:Fluorocarbon Polymers
D000941:Antigens; D003902:Detergents; D008567:Membranes, Artificial; D011145:Polyvinyls; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers