Structural diversity in a conserved cholera toxin epitope involved in ganglioside binding.
Cholera is a widespread disease for which there is no efficient vaccine. A better understanding of the conformational rearrangements at the epitope might be very helpful for the development of a good vaccine. Cholera toxin (CT) as well as the closely related heat-labile toxin from Escherichia coli (LT) are composed of two subunits, A and B, which form an oligomeric assembly AB5. Residues 50-64 on the surface of the B subunits comprise a conserved loop (CTP3), which is involved in saccharide binding to the receptor on epithelial cells. This loop exhibits remarkable conformational plasticity induced by environmental constraints. The crystal structure of this loop is compared in the free and receptor-bound toxins as well as in the crystal and solution structures of a complex with TE33, a monoclonal antibody elicited against CTP3. In the toxins this loop forms an irregular structure connecting a beta-strand to the central alpha-helix. Ser 55 and Gln 56 exhibit considerable conformational variability in the five subunits of the unliganded toxins. Saccharide binding induces a change primarily in Ser 55 and Gln 56 to a conformation identical in all five copies. Thus, saccharide binding confers rigidity upon the loop. The conformation of CTP3 in complex with TE33 is quite different. The amino-terminal part of CTP3 forms a beta-turn that fits snugly into a deep binding pocket on TE33, in both the crystal and NMR-derived solution structure. Only 8 and 12 residues out of 15 are seen in the NMR and crystal structures, respectively. Despite these conformational differences, TE33 is cross-reactive with intact CT, albeit with a thousandfold decrease in affinity. This suggests a different interaction of TE33 with intact CT.
Shoham
M
M
Case Western Reserve University, School of Medicine, Department of Biochemistry, Cleveland, Ohio 44106-4935, USA.
Scherf
T
T
Anglister
J
J
Levitt
M
M
Merritt
E A
EA
Hol
W G
WG
D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology / Q000378:metabolism; D001427:Bacterial Toxins / Q000737:chemistry; D002772:Cholera Toxin / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D002951:Citrates / Q000737:chemistry; D003196:Computer Graphics; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D004768:Enterotoxins / Q000737:chemistry; D000939:Epitopes / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D029968:Escherichia coli Proteins; D005732:Gangliosides / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D011487:Protein Conformation
D000906:Antibodies; D001427:Bacterial Toxins; D002951:Citrates; D004768:Enterotoxins; D000939:Epitopes; D029968:Escherichia coli Proteins; D005732:Gangliosides; D010446:Peptide Fragments; C050310:cholera toxin, B subunit (50-64); D002772:Cholera Toxin; C027538:heat-labile enterotoxin, E coli