IL-10 and IL-3 synergize to cause proliferation of human T cells.
Interleukin-3 (IL-3) is a well-documented haemopoietic growth factor, but its effects on T-cell function are less clear. Although this cytokine is a potent growth factor for CD4- CD8- alpha beta T-cell receptor-expressing cells, it has few known effects on single-positive T cells. In this report it is shown that IL-3 synergizes with IL-10 to induced proliferation of T-cell lines and single-positive clones. Specificity was verified using blocking anti-IL-10 and anti-IL-3 neutralizing antibodies. This induction of proliferation was dose dependent. Taken together the results imply that IL-3 can act as a growth factor for T-cell lines and single-positive T-helper type-1 (Th1) CD4+ clones in the presence of a cofactor, IL-10, a cytokine that has been documented as being predominantly a T-cell inhibitory, and not proinflammatory, cytokine.
Cohen
S B
SB
Kennedy Institute of Rheumatology, Hammersmith, London.
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D004357:Drug Synergism; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000276:immunology*; D007377:Interleukin-3 / Q000037:antagonists & inhibitors / Q000276:immunology*; D015847:Interleukin-4 / Q000096:biosynthesis; D008213:Lymphocyte Activation; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D007377:Interleukin-3; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma; D016572:Cyclosporine