41 kilobases of analyzed sequence from the pseudoautosomal and sex-determining regions of the short arm of the human Y chromosome.
Determination of 41.2 kb of Y chromosome genomic sequence has been made from a cosmid that spans the Yp pseudoautosomal boundary and includes 18.5 kb of sequence from the patient-defined sex-determining region of the Y chromosome. An AceDB database of the sequence and the analysis data have been produced as a resource for studies of the evolution and population genetics of the Y chromosome. Comparison of the 18.5 kb from the sex determining region to the sex determining region of mouse does not locate any areas of similarity outside SRY/Sry. Indeed, no coding regions other than those previously reported can be detected anywhere in the 41 kb. The Y-specific and pseudoautosomal portions of this sequence have different repeat sequence and GC contents: this may have relevance both to the events defining the pseudoautosomal boundary and to the course of sequence evolution in the absence of recombination.
Whitfield
L S
LS
Department of Genetics, University of Cambridge, United Kingdom.
Hawkins
T L
TL
Goodfellow
P N
PN
Sulston
J
J
D000818:Animals; D002874:Chromosome Mapping; D003360:Cosmids; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D051379:Mice; D009687:Nuclear Proteins; D012091:Repetitive Sequences, Nucleic Acid; D012732:Sex Determination Analysis; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D014998:Y Chromosome / Q000235:genetics*
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein; C497746:Sry protein, mouse; D014157:Transcription Factors