Isolation of cDNA for a plasma membrane H(+)-ATPase from guard cells of Vicia faba L.
cDNA encoding the plasma membrane H(+)-ATPase of guard cells of Vicia faba L. was isolated. The clone encoded a 105-kDa polypeptide (956 amino acids) that was 79-85% identical in terms of amino acid sequence to other plant H(+)-ATPases. High levels of mRNA explain the high H(+)-ATPase activity of these plasma membranes.
Nakajima
N
N
National Institute for Environmental Studies, Tsukuba, Japan.
Saji
H
H
Aono
M
M
Kondo
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002462:Cell Membrane / Q000201:enzymology*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D007887:Fabaceae / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000166:cytology / Q000201:enzymology*; D010946:Plants, Medicinal; D006180:Proton-Translocating ATPases / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D006180:Proton-Translocating ATPases