Sialylated Lewis(x) and Lewis(a) determinants expression on human neoplastic cell lines: immunocytometric study with the 5th workshop monoclonal antibodies.
The reactivity of monoclonal antibodies from the 5th workshop Selectins/Selectin Ligands panel, directed to the sialylated Lewis(x) and sialylated Lewis(a) determinants, with the human breast carcinoma (BT-20, ZR-75-1 and MDA-MB-468) cell lines, human ovarian carcinoma cell line A2780, fibrosarcoma (HT-1080, B-6FS) and hematopoietic neoplastic (U-937, HL-60, K-562) cell lines were determined with the aid of flow immunocytofluorometry. The examined monoclonal antibodies to sialylated Lewis determinants reacted with examined breast carcinoma, but not with the examined ovarian carcinoma and fibrosarcoma cell lines.
Sedlák
J
J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Hunáková
L
L
Sulíková
M
M
Chorváth
B
B
D000911:Antibodies, Monoclonal; D001943:Breast Neoplasms / Q000276:immunology; D000939:Epitopes / Q000378:metabolism*; D005260:Female; D005354:Fibrosarcoma / Q000276:immunology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000276:immunology; D007983:Lewis Blood Group Antigens / Q000276:immunology*; D016256:Lewis X Antigen / Q000378:metabolism*; D008545:Melanoma / Q000276:immunology; D009369:Neoplasms / Q000276:immunology*; D010051:Ovarian Neoplasms / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen