Development of cortical fibres into the gracile nucleus of the rat: an ultrastructural study.
A study was made on the development of cortical synapses in the gracile nucleus of rats using degeneration methods. A total of 46 animals, 1 adult and 45 neonates whose ages varied from 1 to 7 days, had the right somatosensory motor cortex destroyed. The survival period varied from 1 to 30 days. Identification of axonal terminals in the gracile nucleus was also achieved by tracing the cortical fibres of 1 adult rat using horseradish peroxidase-wheat germ agglutinin (HRP-WGA). Degenerating axodendritic and axosomatic terminals that originated from cortical fibres were seen in the adult animal which survived 2 days. Their origin was confirmed by the presence of HRP-WGA inside the terminals. Light or electron microscopic changes were not seen, and in particular, the gracile nucleus was not smaller than in the control adult animals which survived 30 days or in neonates which survived 8-30 days, consistent with the small component of cortifugal fibres believed to terminate in secondary sensory nuclei. In neonates that survived a shorter period, terminal degeneration was only seen in cases operated at 4 days and later, indicating that cortical axons do not synapse in the gracile nucleus until postnatal day 4. Our results provide further support for the existence of a period in which the fibres approach their target but do not penetrate it to form synaptic junctions during the growth of cortical axons toward their targets, since previous studies have demonstrated that cortical fibres persisting to adulthood decussate completely at the level of the medulla at 12-36 h after birth.(ABSTRACT TRUNCATED AT 250 WORDS)
Chimelli
L
L
Department of Pathology, Medical School of Ribeirão Prêto, SP, Brazil.
Bello
A R
AR
Scaravilli
F
F
D000818:Animals; D000831:Animals, Newborn; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development*; D006735:Horseradish Peroxidase; D008526:Medulla Oblongata / Q000166:cytology / Q000254:growth & development* / Q000648:ultrastructure; D009044:Motor Cortex / Q000502:physiology; D009410:Nerve Degeneration / Q000502:physiology; D009412:Nerve Fibers / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013003:Somatosensory Cortex / Q000502:physiology; D013569:Synapses / Q000502:physiology; D016707:Tissue Fixation; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins
D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; D014909:Wheat Germ Agglutinins; D006735:Horseradish Peroxidase