title: A specific immunoradiometric assay of cationic trypsin(ogen) that does not recognize trypsin-alpha-1-proteinase inhibitor complex.
abstract: An immunoradiometric assay using two monoclonal antibodies directed to human trypsin 1 was developed for measuring trypsin(ogen) in biological fluids. The assay is different from other assays in that it is specific for cationic trypsinogen and does not recognize the alpha-1-proteinase inhibitor-trypsin complex. It can be used as a complement to classical immunoassays to characterize trypsinogen activation in pathological cases. The evaluation and the specificity of the assay are presented.
authors.affiliation: Centre hospitalier Lyon-Sud, Pierre-Bénite, France.
authors.forename: P
authors.initials: P
authors.lastname: Lafont
authors.forename: O
authors.initials: O
authors.lastname: Guy-Crotte
authors.forename: C
authors.initials: C
authors.lastname: Paulin
authors.forename: D
authors.initials: D
authors.lastname: Galvain
authors.forename: S
authors.initials: S
authors.lastname: Mertani
authors.forename: C
authors.initials: C
authors.lastname: Figarella
authors.forename: Y
authors.initials: Y
authors.lastname: Barbier
mesh_terms: D000510:Alpha-Globulins / Q000032:analysis*; D000653:Amniotic Fluid / Q000201:enzymology; D000818:Animals; D006801:Humans; D015592:Immunoradiometric Assay / Q000379:methods*; D010189:Pancreatic Juice / Q000201:enzymology; D011863:Radioimmunoassay; D051381:Rats; D013552:Swine; D014357:Trypsin / Q000032:analysis* / Q000097:blood; D014361:Trypsin Inhibitors / Q000032:analysis* / Q000097:blood; D014362:Trypsinogen / Q000032:analysis* / Q000097:blood
chemical_list: D000510:Alpha-Globulins; D014361:Trypsin Inhibitors; C011877:inter-alpha-inhibitor; D014362:Trypsinogen; D014357:Trypsin