Inflammatory arthritis: imaging of endothelial cell activation with an indium-111-labeled F(ab')2 fragment of anti-E-selectin monoclonal antibody.
OBJECTIVE
To use imaging performed with an indium-111-labeled F(ab')2 fragment of monoclonal antibody that recognizes E-selectin (1.2B6) to evaluate inflammation.


METHODS
After the in vivo immunoreactivity of In-111-F(ab')2 was verified, imaging experiments were conducted on pigs with induced arthritis of the right knee. In-111-labeled 1.2B6-F(ab')2, technetium-99m-hexamethylpropyleneamine oxime-labeled polymorphonuclear leukocytes (PMNs), and iodine-125-MOPC 21-labeled F(ab')2 (the control) were administered by means of intravenous injection. Scintigrams were obtained until 20 hours after injection, and tissue sampling was performed.


RESULTS
Scintigrams showed uptake of 1.2B6 in the right knee but only faint filling of the joint space with PMNs. The right knee-to-left knee ratios on images obtained 20 hours after injection of the tracers were 10.8 +/- 3.6 for 1.2B6 and 1.1 +/- 0.4 for PMNs (P < .05).


CONCLUSIONS
Endothelial cell immunotargeting with a radiolabeled anti-E-selectin F(ab')2 monoclonal antibody is feasible for use in imaging of inflammation.
Jamar
F
F
Department of Diagnostic Radiology, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
Chapman
P T
PT
Harrison
A A
AA
Binns
R M
RM
Haskard
D O
DO
Peters
A M
AM
D000818:Animals; D001169:Arthritis, Experimental / Q000000981:diagnostic imaging*; D015815:Cell Adhesion Molecules / Q000276:immunology*; D019040:E-Selectin; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins / Q000276:immunology*; D009504:Neutrophils; D015609:Organotechnetium Compounds; D010091:Oximes; D016719:Radioimmunodetection; D013552:Swine; D019690:Technetium Tc 99m Exametazime
D015815:Cell Adhesion Molecules; D019040:E-Selectin; D007205:Indium Radioisotopes; D007457:Iodine Radioisotopes; D008562:Membrane Glycoproteins; D015609:Organotechnetium Compounds; D010091:Oximes; D019690:Technetium Tc 99m Exametazime