Structural studies of the O-specific chain and a core hexasaccharide of Hafnia alvei strain 1192 lipopolysaccharide.
The structure of the O-specific side-chain and a core hexasaccharide of the Hafnia alvei strain 1192 lipopolysaccharide has been investigated. Methylation analysis, NMR spectroscopy, MALDI-TOF spectrometry, and various specific chemical degradations were the principal methods used. It is concluded that the polysaccharide is composed of hexasaccharide repeating-units having the following structure which is partially O-acetylated in the 2-position of the --> 4)-alpha-D-Glc pA-(1-->(70%) and on different positions of the L-Rha residues (50%). [Formula: see text] The core hexasaccharide was found to have the following structure: [Formula: see text]
Jachymek
W
W
L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Science, Wroclaw.
Petersson
C
C
Helander
A
A
Kenne
L
L
Lugowski
C
C
Niedziela
T
T
D000117:Acetylglucosamine; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004755:Enterobacteriaceae / Q000737:chemistry*; D005947:Glucose; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008969:Molecular Sequence Data; D019081:O Antigens; D009844:Oligosaccharides / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012210:Rhamnose; D012266:Ribose; D017421:Sequence Analysis
D019081:O Antigens; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D012266:Ribose; D005947:Glucose; D012210:Rhamnose; D000117:Acetylglucosamine