Bacterial expression and purification of human protein prenyltransferases using epitope-tagged, translationally coupled systems.
Omer
C A
CA
Department of Cancer Research, Merck Research Laboratories, West Point, Pennsylvania 19486, USA.
Diehl
R E
RE
Kral
A M
AM
D019883:Alkyl and Aryl Transferases; D000595:Amino Acid Sequence; D001483:Base Sequence; D002852:Chromatography, Ion Exchange / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers; D000939:Epitopes; D004926:Escherichia coli; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D014176:Protein Biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D014166:Transferases / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification
D017931:DNA Primers; D000939:Epitopes; D007202:Indicators and Reagents; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D014166:Transferases; D019883:Alkyl and Aryl Transferases; C083291:geranylgeranyltransferase type-I; C064911:p21(ras) farnesyl-protein transferase