title: A comparative electron microscopic study of chemical techniques for preservation of auditory ossicles as homografts.
abstract: The ultrastructure of preserved ossicles at different shelf lives was compared. Homograft ossicles were harvested and preserved in 70% alcohol, 1:5000 Cialit (sodium-2-ethyl-mercuri-mercapto-benzoxazole-5-carboxylate), and buffered 4% formaldehyde at pH 7. The ultrastructure of each ossicle was studied by both scanning and transmission electron microscopy and was compared to that of fresh controls placed in 0.9 N saline solution. The results indicated clearly the superiority of 70% alcohol as a long-term preservative and 1:5000 Cialit as a short-term preservative.
authors.affiliation: Department of Otorhinolaryngology Faculty of Medicine, Ain-Shams University, Abbassia, Cairo, Egypt.
authors.forename: M
authors.initials: M
authors.lastname: el-Kahky
authors.forename: F
authors.initials: F
authors.lastname: Linthicum
authors.forename: A
authors.initials: A
authors.lastname: Sarwat
authors.forename: H
authors.initials: H
authors.lastname: el-Mehairy
authors.forename: H
authors.initials: H
authors.lastname: Wahba
mesh_terms: D002920:Cialit; D003094:Collagen / Q000648:ultrastructure; D004429:Ear Ossicles / Q000473:pathology / Q000637:transplantation*; D000431:Ethanol; D005557:Formaldehyde; D006085:Graft Survival / Q000502:physiology; D006801:Humans; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D012965:Sodium Chloride; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D002920:Cialit; D005557:Formaldehyde; D000431:Ethanol; D012965:Sodium Chloride; D003094:Collagen