title: De novo development and self-replenishment of B cells.
abstract: Previous studies distinguished two murine B cell lineages: the conventional lineage, which comprises the majority of B cells, and the Ly-1 B lineage (B-1a), which represents a small percentage of total adult B cells. A third subset, B-1b cells, shares many properties with B-1a cells, including the characteristic ability to self-replenish, but does not express Ly-1 (CD5). Reconstitution studies presented here show that (i) although the B220- population in adult spleen and bone marrow contains very little progenitor activity for B-1a cells, it can reconstitute roughly half the normal number of B-1b cells; (ii) B-1 progenitors present in adult bone marrow and spleen function at low levels in adult animals; (iii) peritoneal B-1 cells (principally B-1b) that develop following bone marrow transfer, like B-1 cells from normal animals, are capable of substantial self-replenishment; and (iv) conventional B cells do not expand (self-replenish) in adoptive recipients, although they can persist for long periods. Collectively, these progenitor and self-replenishment characteristics provide a developmental base for distinguishing B-1a, B-1b and conventional B cells.
authors.affiliation: Beckman Center B-007, Department of Genetics, Stanford University Medical Center, CA 94305-5125, USA.
authors.forename: A B
authors.initials: AB
authors.lastname: Kantor
authors.forename: A M
authors.initials: AM
authors.lastname: Stall
authors.forename: S
authors.initials: S
authors.lastname: Adams
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: L A
authors.initials: LA
authors.lastname: Herzenberg
mesh_terms: D000367:Age Factors; D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D016175:B-Lymphocyte Subsets / Q000166:cytology*; D001854:Bone Marrow Cells; D018956:CD5 Antigens; D005260:Female; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D007075:Immunoglobulin M / Q000378:metabolism; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D013154:Spleen / Q000166:cytology
chemical_list: D015703:Antigens, CD; D018956:CD5 Antigens; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell