Molecular analysis of the Cip1/Waf1 (p21) gene in diverse types of human tumors.
We have screened for mutations in the Cip1/Waf1 gene using Southern blot analysis and the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method in diverse human tumors. Seven of 102 (7%) human tumor samples were identified to have point mutations within the coding region of the Cip1/Waf1 gene. Two of the seven mutated cases showed gene rearrangements. These results suggest that the frequency of genetic alterations in the Cip1/Waf1 gene is relatively low in comparison with several known tumor suppressor genes.
Watanabe
H
H
Department of Clinical Pathology, Showa University of Medicine, Tokyo, Japan.
Fukuchi
K
K
Takagi
Y
Y
Tomoyasu
S
S
Tsuruoka
N
N
Gomi
K
K
D001483:Base Sequence; D015139:Blotting, Southern; D015342:DNA Probes; D015321:Gene Rearrangement; D016147:Genes, Tumor Suppressor; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction
D015342:DNA Probes