Loss of chromosome 17 loci in prostate cancer detected by polymerase chain reaction quantitation of allelic markers.
Using a polymerase chain reaction/microsatellite marker system, we demonstrated that 6 of 22 (27%) clinical stage B (early) primary prostate tumors showed loss of heterozygosity at one or more of five loci on chromosome 17. The sensitivity of this study was increased by use of a PhosphorImager and statistical analysis of replicate tumor-normal DNA pairs. Two patients showed tumor-specific interstitial loss at a locus in close proximity to the familial breast cancer gene BRCA1. These findings suggest that genes on the proximal long arm of chromosome 17 play a pivotal role in the early development of at least a subset of prostatic tumors.
Brothman
A R
AR
Department of Human Genetics, University of Utah School of Medicine, Salt Lake City 84132, USA.
Steele
M R
MR
Williams
B J
BJ
Jones
E
E
Odelberg
S
S
Albertsen
H M
HM
Jorde
L B
LB
Rohr
L R
LR
Stephenson
R A
RA
D000483:Alleles; D016022:Case-Control Studies; D002872:Chromosome Deletion; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D011471:Prostatic Neoplasms / Q000235:genetics*
D005819:Genetic Markers