title: Expression of the ROS1 oncogene for tyrosine receptor kinase in adult human meningiomas.
abstract: Oncogenes have been implicated in the promotion and progression of cancer in humans. Expression of the ROS1 oncogene, a member of the receptor tyrosine kinase superfamily, was examined in human meningiomas by coupled reverse transcription and polymerase chain reaction (RT-PCR) assays. Two sets of region-specific oligonucleotides, specific for different regions of the ROS1 messenger ribonucleic acid (mRNA), were used in RT-PCR assays to independently examine ROS1 transcripts from primary human meningiomas. ROS1 was expressed at high levels in approximately 55% (17 of 31) of the meningiomas examined, but not expressed in non-neoplastic brain samples. The commonplace expression of the ROS1 oncogene in meningiomas suggests a role for this oncogene in the etiology of these tumors.
authors.affiliation: Department of Microbiology and Immunology, University of Tennessee, Memphis 38163, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: Zhao
authors.forename: S
authors.initials: S
authors.lastname: Sharma
mesh_terms: D015870:Gene Expression / Q000235:genetics; D006801:Humans; D008577:Meningeal Neoplasms / Q000235:genetics* / Q000378:metabolism; D008579:Meningioma / Q000235:genetics* / Q000378:metabolism; D009857:Oncogenes / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
chemical_list: D020794:Receptor Protein-Tyrosine Kinases