title: Monitoring biological effects of contamination in marine fish along French coasts by measurement of ethoxyresorufin-O-deethylase activity.
abstract: The use of bioindicators to evaluate exposure to the biological effects of chemical pollutants in marine organisms constitutes a new tool in the monitoring field. The establishment of a North Sea monitoring network in 1991, involving such international organizations as the North Sea Task Force, the International Council for the Exploration of the Sea, and the Intergovernmental Oceanography Commission, led French researchers to develop an enzymatic biomarker to monitor biological effects within the National Observation Network. The biomarker, ethoxyresorufin-O-deethylase (EROD), dependent on the CP450 system, has been monitored biannually since 1992 in several species of fish (Callionymus lyra, Limanda limanda, Serranus sp., Mullus barbatus) in two coastal sites particularly exposed to industrial and domestic pollution. A rapid method is used to assay EROD enzymatic activity determined along a pollution gradient, and results are interpreted on a microplate reader. The strategy of this approach is to assess the effects on the marine ecosystem during prolonged exposure to specific pollutants such as polyaromatic hydrocarbons, polychlorinated biphenyls, and dioxins.
authors.affiliation: IFREMER, Rue de l'Ile d'Yeu, Nantes, France.
authors.forename: T
authors.initials: T
authors.lastname: Burgeot
authors.forename: G
authors.initials: G
authors.lastname: Bocquené
authors.forename: G
authors.initials: G
authors.lastname: Pingray
authors.forename: D
authors.initials: D
authors.lastname: Godefroy
authors.forename: J
authors.initials: J
authors.lastname: Legrand
authors.forename: J
authors.initials: J
authors.lastname: Dimeet
authors.forename: F
authors.initials: F
authors.lastname: Marco
authors.forename: F
authors.initials: F
authors.lastname: Vincent
authors.forename: Y
authors.initials: Y
authors.lastname: Henocque
authors.forename: H O
authors.initials: HO
authors.lastname: Jeanneret
mesh_terms: D000818:Animals; D019363:Cytochrome P-450 CYP1A1; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000187:drug effects; D004147:Dioxins / Q000494:pharmacology; D004784:Environmental Monitoring / Q000379:methods*; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D005399:Fishes / Q000378:metabolism*; D005602:France; D008297:Male; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000187:drug effects; D011078:Polychlorinated Biphenyls / Q000494:pharmacology; D011083:Polycyclic Compounds / Q000494:pharmacology; D012621:Seasons; D012623:Seawater; D014874:Water Pollutants, Chemical / Q000494:pharmacology*
chemical_list: D004147:Dioxins; D011083:Polycyclic Compounds; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D011078:Polychlorinated Biphenyls; D010088:Oxidoreductases; D019363:Cytochrome P-450 CYP1A1