title: Histamine release from cord blood basophils is influenced by plasma IgE concentration, osmolarity, gestational age at birth and atopic disposition.
abstract: To characterize the normal values of histamine release from basophil leucocytes in cord blood after stimulation with both IgE-mediating and non-IgE-mediating secretagogues, a population of 1,684 newborn infants was studied using a microfiber-based method for detecting histamine release. A wide variation in anti-IgE and Concanavalin A-induced histamine release in the population was found. An increase in osmolarity in the release media selectively enhanced the maximal IgE-mediated histamine release, in addition to an increase in sensitivity in the dose response for anti-IgE and Concanavalin A. Both anti-IgE and Concanavalin A mediated histamine release were found to be significantly associated with the concentration of IgE in cord blood. Furthermore, an increased sensitivity to the IgE-mediated response and a close correlation between anti-IgE and Concanavalin A histamine release were found in the group of newborns with high IgE levels. Gestational age at birth and histamine release after stimulation with both IgE-mediating, and non-IgE mediating secretagogues were found to be significantly related, thus suggesting a continuing maturation of the human basophil leucocytes in the last weeks of gestation. Parental atopic disposition also affected basophil releasability in cord blood.
authors.affiliation: Allergy Research Laboratory, University Hospital, Aarthus, Denmark.
authors.forename: T
authors.initials: T
authors.lastname: Bjerke
authors.forename: M
authors.initials: M
authors.lastname: Hedegaard
authors.forename: T B
authors.initials: TB
authors.lastname: Henriksen
authors.forename: B W
authors.initials: BW
authors.lastname: Nielsen
authors.forename: P O
authors.initials: PO
authors.lastname: Schiøtz
mesh_terms: D001491:Basophils / Q000378:metabolism*; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D005865:Gestational Age; D006636:Histamine Release / Q000502:physiology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000235:genetics / Q000276:immunology*; D007070:Immunoglobulin A / Q000097:blood; D007073:Immunoglobulin E / Q000097:blood*; D066298:In Vitro Techniques; D007231:Infant, Newborn / Q000276:immunology*; D008297:Male; D009994:Osmolar Concentration
chemical_list: D007070:Immunoglobulin A; D007073:Immunoglobulin E