Resuscitation of brain neurons in the presence of Ca2+ after toxic NMDA-receptor activity.
Cultured cerebellar granule cells were subjected to toxic activation of the NMDA receptor that was terminated by MK-801. Subsequent resuscitation experiments were mostly conducted in the presence of a physiological concentration of Ca2+. Addition of pyruvate and inorganic phosphate, in addition to glucose, which was always present, rescued approximately 40% of the dying neurons. La3+ and ruthenium red were also effective resuscitating agents. The combination of pyruvate, inorganic phosphate, and ruthenium red rescued 65% of the dying neurons. Parallel studies with 45Ca indicated that La3+ and ruthenium red facilitated the decrease of 45Ca in the neurons, whereas inorganic phosphate, supported by energy-yielding pyruvate, formed perhaps, a less harmful Ca complex inside the neurons.
Eimerl
S
S
Department of Biological Chemistry, Hebrew University of Jerusalem, Israel.
Schramm
M
M
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D003470:Culture Media; D007811:Lanthanum / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D010710:Phosphates / Q000494:pharmacology; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology*; D012430:Ruthenium Red / Q000494:pharmacology
D003470:Culture Media; D010710:Phosphates; D016194:Receptors, N-Methyl-D-Aspartate; D012430:Ruthenium Red; D007811:Lanthanum; D002118:Calcium