Flow cytometric analysis of cell-surface and intracellular antigens in leukemia diagnosis.
New technology allows highly sensitive flow cytometric detection and quantitative analysis of intracellular antigens in normal and malignant hemopoietic cells. With this technology, the earliest stages of myeloid and lymphoid differentiation can easily and reliably be identified using antibodies directed against (pro-)myeloperoxidase/MPO, CD22 and CD3 antigens, respectively. Particularly for the analysis of undifferentiated acute myeloblastic leukemia (AML) cells, the immunological demonstration of intracellular MPO or its enzymatically inactive proforms is highly relevant, since other myeloid marker molecules such as CD33, CD13, or CDw65 are either not restricted to the granulomonocytic lineage or appear later in differentiation. By combining MPO staining with staining for lactoferrin (LF), undifferentiated cells can be distinguished from the granulomonocytic maturation compartment in bone marrow, since LF is selectively expressed from the myelocyte stage of differentiation onward. The list of informative intracellular antigens to be used in leukemia cell analysis will certainly expand in the near future. One candidate, intracellular CD68, has already been tested by us, and results are presented. Also dealt within this article are surface marker molecules not (as yet) widely used in leukemia cell analysis but with the potential to provide important additional information. Among them are the surface structures CD15, CD15s, CDw65, CD79a (MB-1), CD79b (B29), CD87 (uPA-R), and CD117 (c-kit).
Knapp
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Institut für Immunologie der Universität Wien, Austria.
Strobl
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H
Majdic
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D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D014408:Biomarkers, Tumor / Q000032:analysis*; D017252:CD3 Complex / Q000032:analysis; D051925:CD79 Antigens; D015815:Cell Adhesion Molecules; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods; D007781:Lactoferrin / Q000032:analysis; D037102:Lectins; D007938:Leukemia / Q000175:diagnosis* / Q000276:immunology; D016256:Lewis X Antigen / Q000032:analysis; D008562:Membrane Glycoproteins / Q000032:analysis; D009195:Peroxidase / Q000032:analysis; D011518:Proto-Oncogene Proteins / Q000032:analysis; D019009:Proto-Oncogene Proteins c-kit; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D011956:Receptors, Cell Surface / Q000032:analysis; D016184:Receptors, Colony-Stimulating Factor / Q000032:analysis; D055293:Receptors, Urokinase Plasminogen Activator; D051918:Sialic Acid Binding Ig-like Lectin 2; D000097382:CD68 Molecule
D015703:Antigens, CD; D018952:Antigens, CD34; D000944:Antigens, Differentiation, B-Lymphocyte; D015214:Antigens, Differentiation, Myelomonocytic; D000945:Antigens, Differentiation, T-Lymphocyte; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D014408:Biomarkers, Tumor; D017252:CD3 Complex; D051925:CD79 Antigens; D015815:Cell Adhesion Molecules; D007781:Lactoferrin; D037102:Lectins; D016256:Lewis X Antigen; D008562:Membrane Glycoproteins; D009195:Peroxidase; D011518:Proto-Oncogene Proteins; D019009:Proto-Oncogene Proteins c-kit; D020794:Receptor Protein-Tyrosine Kinases; D011947:Receptors, Antigen, B-Cell; D011956:Receptors, Cell Surface; D016184:Receptors, Colony-Stimulating Factor; D055293:Receptors, Urokinase Plasminogen Activator; D051918:Sialic Acid Binding Ig-like Lectin 2; C497867:CD22 protein, human; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C497881:CD79A protein, human; C524898:PLAUR protein, human