title: Isolation and characterization of a rat testis glycosphingolipid based on gangliotetraosylceramide and having a blood group B determinant extended with beta 3-linked N-acetylgalactosamine.
abstract: Monoclonal antibody 14.2, directed against the GalNAc beta 3Gal alpha 3Gal beta epitope, was used to monitor the isolation of a novel glycosphingolipid from rat testes. Structural analysis was performed by mass spectrometry, proton NMR spectroscopy, gas chromatography-mass spectrometry after degradation, and exoglycosidase digestion. It was concluded that the structure was [formula: see text] This is the first report on an extended blood group B determinant.
authors.affiliation: Department of Medical Biochemistry, Göteborg University, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Teneberg
authors.forename: P A
authors.initials: PA
authors.lastname: Jovall
authors.forename: H
authors.initials: H
authors.lastname: Karlsson
authors.forename: H O
authors.initials: HO
authors.lastname: Sjögren
authors.forename: T
authors.initials: T
authors.lastname: Brodin
mesh_terms: D000017:ABO Blood-Group System / Q000276:immunology*; D000116:Acetylgalactosamine / Q000737:chemistry*; D000818:Animals; D000937:Antigen-Antibody Reactions; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002849:Chromatography, Gas; D000939:Epitopes; D005732:Gangliosides; D006028:Glycosphingolipids / Q000032:analysis* / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D051381:Rats; D011920:Rats, Inbred WF; D013737:Testis / Q000737:chemistry*
chemical_list: D000017:ABO Blood-Group System; D000939:Epitopes; C011258:G(A1) ganglioside; D005732:Gangliosides; D006028:Glycosphingolipids; D000116:Acetylgalactosamine