2-Methoxy-4-nitroaniline is a selective inducer of cytochrome P450IA2 (CYP1A2) in rat liver.
Male F344 rats were treated with a chemical (aniline, nitrobenzene, 2-methoxy-p-phenylenediamine, 2-methoxy-4-nitroaniline or 2-methoxy-4-nitroazobenzene) produced by the azo-reduction and/or N-oxidation of 2-methoxy-4-amino-azo-benzene, a selective inducer of cytochrome P450IA2 (CYP1A2), and their effects on the induction of CYP1A enzymes in the liver were examined in terms of the protein and mRNA amounts. 2-Methoxy-4-nitroaniline and 2-methoxy-4-nitroazo-benzene, but not other compounds tested, induced CYP1A enzymes, especially CYP1A2, as assessed by Western blotting and Northern blotting. It is noteworthy that 2-methoxy-4-nitroaniline was more selective than 2-methoxy-4-aminoazobenzene for induction of CYP1A2, and it has the smallest molecular size among the known CYP1A2 inducers.
Degawa
M
M
Department of Hygineic Chemistry, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
Nakayama
M
M
Yoshinari
K
K
Hashimoto
Y
Y
D000814:Aniline Compounds / Q000494:pharmacology*; D000818:Animals; D001483:Base Sequence; D019388:Cytochrome P-450 CYP1A2; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D017931:DNA Primers / Q000737:chemistry; D004790:Enzyme Induction / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344
D000814:Aniline Compounds; D017931:DNA Primers; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D019388:Cytochrome P-450 CYP1A2