title: Analysis of immunoglobulin gene rearrangements in single B cells.
abstract: The polymerase chain reaction allows the characterization of RNA and DNA sequences in samples as small as a single cell. The recent development of amplification systems designed to isolate rearranged immunoglobulin genes from single B lineage cells has provided a powerful tool to investigate various aspects of B-cell development.
authors.affiliation: Institute for Genetics, University of Cologne, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Ehlich
authors.forename: R
authors.initials: R
authors.lastname: Küppers
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry*; D015322:Gene Rearrangement, B-Lymphocyte; D005803:Genes, Immunoglobulin; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*
chemical_list: D007135:Immunoglobulin Variable Region