Mechanisms of regulatory volume decrease in nonpigmented human ciliary epithelial cells.
To study the net solute and water efflux pathways of the ciliary epithelium we employed a cultured human NPE cell line. Because of the possible relationship between transepithelial ion and water flux and cell volume regulation, the ion efflux pathways mediating regulatory volume decrease (RVD) were investigated. Osmotic swelling of NPE cells was followed by a volume recovery. Volume recovery was K+ dependent and inhibited by K+ channel blockers such as quinine (1 mM). After osmotic swelling, a Cl(-)-dependent membrane depolarization occurred that was inhibited by Cl- channel blockers such as 5-nitro-2-(3-phenylpropylamino)benzoic acid (100 microM) or Ca2+ chelators such as ethylene glycolbis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA, 2.0 mM). Cell swelling was also accompanied by an increase in intracellular Ca2+ concentration ([Ca2+]i) of approximately 200 nM. The swelling-induced rise in [Ca2+]i and RVD were diminished in the presence of 10 microM La3+, 50 nM 12-O-tetradecanoylphorbol 13-acetate, and nominally Ca(2+)-free medium. Near total blockage of RVD occurred after pretreatment of NPE cells with Ca(2+)-free EGTA-1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) acetoxymethyl ester-containing solutions. The inhibition of RVD by EGTA-BAPTA treatment was overcome by increasing K+ conductance with gramicidin. The above findings indicate that RVD in NPE cells is mediated by separate K+ and Cl- conductances (channels). These data also show that swelling-induced increases in [Ca2+]i help modulate net ion efflux during regulation.
Adorante
J S
JS
Allergan, Inc., Irvine 92715.
Cala
P M
PM
D002118:Calcium / Q000378:metabolism; D048429:Cell Size; D002478:Cells, Cultured; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000502:physiology; D002712:Chlorides / Q000494:pharmacology; D002923:Cilia; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004553:Electric Conductivity; D004847:Epithelial Cells; D006096:Gramicidin / Q000494:pharmacology; D006801:Humans; D007038:Hypotonic Solutions; D007811:Lanthanum / Q000494:pharmacology; D008564:Membrane Potentials; D015221:Potassium Channels / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D018118:Chloride Channels; D002712:Chlorides; D007038:Hypotonic Solutions; D015221:Potassium Channels; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; D006096:Gramicidin; D004533:Egtazic Acid; D007811:Lanthanum; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium