Human tumour necrosis factor-alpha inhibits glucose transport in cultured Ehrlich ascites tumour cells.
Human recombinant tumour necrosis factor-alpha (rhTNF-alpha) arrested the growth of Ehrlich ascites tumour (EAT) cells in vitro. It suppressed cellular glucose uptake and decreased the membrane density of glucose transporters as measured by glucose-reversible cytochalasin B binding. The glucose transporters' affinity for substrate was also reduced. However, rhTNF-alpha treatment exerted no effect on the phosphoribosyl pyrophosphate level in EAT cells. The role of rhTNF-alpha on the inhibition of glucose transport of tumour cells is discussed.
Fung
K P
KP
Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T.
Lam
W P
WP
Choy
Y M
YM
Lee
C Y
CY
D000818:Animals; D001665:Binding Sites; D001692:Biological Transport / Q000187:drug effects; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003571:Cytochalasin B / Q000378:metabolism; D003847:Deoxyglucose / Q000493:pharmacokinetics; D005947:Glucose / Q000493:pharmacokinetics*; D006801:Humans; D007700:Kinetics; D009004:Monosaccharide Transport Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010754:Phosphoribosyl Pyrophosphate / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D009004:Monosaccharide Transport Proteins; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D003571:Cytochalasin B; D010754:Phosphoribosyl Pyrophosphate; D003847:Deoxyglucose; D005947:Glucose