title: Lipids and proteins in the Rathke's gland secretions of the North American mud turtle (Kinosternon subrubrum).
abstract: Lipids and proteins in the Rathke's gland secretions of the North American mud turtle (Kinosternon subrubrum, Kinosternidae) were analyzed by gas chromatography-mass spectrometry (GC-MS) and SDS-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. Analysis by GC-MS indicates 2,3-dihydroxypropanal and C3-C24 free or esterified fatty acids. Analysis by SDS-PAGE indicates a major protein component with an approximate molecular mass of 60 kDa and minor components ranging from ca. 23 to 34 kDa. The major component of K. subrubrum glandular secretions exhibits a mobility that matches that of the Kemp's ridley sea turtle (Lepidochelys kempi, Cheloniidae), suggesting that these proteins are evolutionarily conserved.
authors.affiliation: University of Texas Medical School at Houston 77225, USA.
authors.forename: W E
authors.initials: WE
authors.lastname: Seifert
authors.forename: S W
authors.initials: SW
authors.lastname: Gotte
authors.forename: T L
authors.initials: TL
authors.lastname: Leto
authors.forename: P J
authors.initials: PJ
authors.lastname: Weldon
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D008055:Lipids / Q000032:analysis*; D008297:Male; D008970:Molecular Weight; D009656:North America; D011506:Proteins / Q000032:analysis*; D014426:Turtles / Q000378:metabolism*
chemical_list: D008055:Lipids; D011506:Proteins