title: Production of thyroid-stimulating antibodies in mice by immunization with T-cell epitopes of human thyrotropin receptor.
abstract: We examined whether mice, immunized with TSH receptor (TSH-R) peptides, which are known to be T-cell epitopes in patients with Graves' disease, would show thyroid-stimulating antibody (TSAb). We immunized DBA/1J mice with TSH-R peptide amino acids 132-150, 145-163, 158-176, and 172-186 and with a pool of these four peptides. The antibodies produced in these mice were evaluated by measurement of TSAb activity using Chinese hamster ovary cells expressing human TSH-R. Seven of 20 mice showed TSAb activity that could be partially blocked with TSH-R peptides. To assess the role of T-cell epitope-specific T-cells in the production of TSAb, we transferred a T-cell line developed from a TSAb-positive mouse to other syngeneic DBA/1J mice. Two of 4 recipient mice showed TSAb activities. These findings suggest that specific T-cell epitopes of TSH-R play a crucial role in the production of TSAb.
authors.affiliation: Thyroid Study Unit, University of Chicago, Illinois 60637.
authors.forename: Y
authors.initials: Y
authors.lastname: Hidaka
authors.forename: V
authors.initials: V
authors.lastname: Guimaraes
authors.forename: M
authors.initials: M
authors.lastname: Soliman
authors.forename: T
authors.initials: T
authors.lastname: Yanagawa
authors.forename: Y
authors.initials: Y
authors.lastname: Okamoto
authors.forename: J
authors.initials: J
authors.lastname: Quintans
authors.forename: L J
authors.initials: LJ
authors.lastname: DeGroot
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016466:CHO Cells / Q000378:metabolism; D006224:Cricetinae; D000242:Cyclic AMP / Q000096:biosynthesis; D000939:Epitopes / Q000276:immunology*; D015870:Gene Expression; D007114:Immunization; D018828:Immunoglobulins, Thyroid-Stimulating / Q000096:biosynthesis*; D051379:Mice; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D011989:Receptors, Thyrotropin / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection
chemical_list: D000939:Epitopes; D018828:Immunoglobulins, Thyroid-Stimulating; D011989:Receptors, Thyrotropin; D000242:Cyclic AMP