title: Heterogeneity of rRNA gene restriction patterns of multiresistant serotype 6B Streptococcus pneumoniae strains.
abstract: Three multiresistant serotype 6B Streptococcus pneumoniae strains were isolated from the middle ear fluids of children undergoing tympanostomy in Atlanta. Because multiresistant 6B pneumococci have been reported to spread from a single clone, the three isolates were compared with 13 other multiresistant 6B pneumococci by hybridization of endonuclease-restricted DNA fragments with a digoxigenin-labeled cDNA probe complementary to 16 and 23S rRNAs (ribotyping). The ear isolates were heterogeneous, whereas six of the other pneumococcal isolates were alike, indicating a need for additional studies to determine the possibility of clonal spread.
authors.affiliation: Biology Department, American University of Beirut, Lebanon.
authors.forename: H
authors.initials: H
authors.lastname: Harakeh
authors.forename: G S
authors.initials: GS
authors.lastname: Bosley
authors.forename: J A
authors.initials: JA
authors.lastname: Keihlbauch
authors.forename: B S
authors.initials: BS
authors.lastname: Fields
mesh_terms: D002648:Child; D004269:DNA, Bacterial / Q000032:analysis; D018432:Drug Resistance, Multiple / Q000235:genetics*; D004432:Ear, Middle / Q000382:microbiology; D018740:Genetic Heterogeneity; D006801:Humans; D010033:Otitis Media / Q000382:microbiology*; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics*; D012335:RNA, Ribosomal / Q000235:genetics*; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012335:RNA, Ribosomal