An evaluation of the staining of lymphomas and normal tissues by the rabbit polyclonal antibody to protein gene product 9.5 following non-enzymatic retrieval of antigen.
We have previously described the staining of normal follicle centre lymphoid cells by the rabbit polyclonal antibody to protein gene product 9.5 (PGP9.5), following an antigen unmasking step employing heat pretreatment. Applying this finding to a range of lymphomas to determine whether this phenomenon could have a role in identifying lymphomas of follicle centre origin revealed no relationship between type or grade of lymphoma and staining of neoplastic cells. Testing a range of normal tissues following antigen retrieval displayed increased sensitivity and a greater range of tissue positivity. Immunoblots of gel electrophoresed tonsil and brain extract were performed. Although subjecting these blots to antigen unmasking increased sensitivity, no novel epitopes, in terms of additional bands, appeared. This suggested that antibody specificity remained unaltered.
Langlois
N E
NE
University of Aberdeen, Scotland, U.K.
King
G
G
Herriot
R
R
Thompson
W D
WD
D015153:Blotting, Western; D001921:Brain / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D016591:Histocytological Preparation Techniques; D006689:Hodgkin Disease / Q000201:enzymology; D006801:Humans; D008223:Lymphoma / Q000201:enzymology*; D008228:Lymphoma, Non-Hodgkin / Q000201:enzymology; D008297:Male; D008872:Microwaves; D014066:Palatine Tonsil / Q000201:enzymology; D013194:Staining and Labeling / Q000379:methods*; D013869:Thiolester Hydrolases / Q000032:analysis*; D043222:Ubiquitin Thiolesterase
D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase