Danazol decreases transcription of estrogen receptor gene in human monocytes.
1. Administration of danazol for over one month reduced the levels of estrogen receptor (ER) and its mRNA to approximately 50 and 20%, respectively in monocytes. 2. Danazol did not alter the degradation rate of ER mRNA in monocytes. 3. Danazol decreased the transcription rate of ER gene to approximately 50% in monocytes in a run-on assay. 4. Danazol may release estrogen predominance via the reduction of transcription for ER gene, which leads to the reduction of ER mRNA and ER expressions in monocytes.
Fujimoto
J
J
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
Hori
M
M
Itoh
T
T
Ichigo
S
S
Nishigaki
M
M
Tamaya
T
T
D000328:Adult; D001483:Base Sequence; D001667:Binding, Competitive / Q000187:drug effects; D015139:Blotting, Southern; D003613:Danazol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012194:RNA-Directed DNA Polymerase; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000187:drug effects / Q000235:genetics
D012333:RNA, Messenger; D011960:Receptors, Estrogen; D012313:RNA; D012194:RNA-Directed DNA Polymerase; D003613:Danazol