Flow cytometric analysis of decay-accelerating factor (CD55) on neutrophils from aplastic anaemia patients.
Using a flow cytometric analysis, CD55 (decay-accelerating factor), CD59 and CD58 have been measured on neutrophils from 12 aplastic anaemia (AA) patients who were long-term survivors after immunosuppressive therapy (IS), 17 healthy individuals, four patients with PNH, and six patients with other haematological disorders. The neutrophils from normal control patients and the six patients with other haematological disorders showed 98 +/- 2% (mean +/- SD) positive granulocytes for CD55. Corresponding values were low (12%, 26%, 51% and 58%) on the primarily PNH patients. Among the 12 AA patients examined, seven had normal and five low values (59% in two, 70%, 71% and 82%). Among the five AA patients who showed CD55 neutrophil deficiency, four had showed an incomplete response after the initial IS treatment and the other relapsed following an initial haematological complete response; three cases had a positive Ham's test and two were negative. Our data suggest that the development of PNH clones is a frequent finding in AA long-term survivors, mainly in those who had shown an incomplete response following IS. Neutrophil CD55 expression analysis by flow cytometry could be useful to detect clonal evolution in these patients.
Forés
R
R
Department of Haematology, Clínica Puerta de Hierro, Universidad Autónoma de Madrid, Spain.
Alcocer
M
M
Diez-Martin
J L
JL
Fernandez
M N
MN
D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000276:immunology*; D015703:Antigens, CD / Q000378:metabolism*; D018958:CD55 Antigens; D018968:CD58 Antigens; D018957:CD59 Antigens; D003169:Complement Inactivator Proteins / Q000378:metabolism*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008875:Middle Aged; D009503:Neutropenia / Q000276:immunology; D009504:Neutrophils / Q000378:metabolism*
D015703:Antigens, CD; D018958:CD55 Antigens; D018968:CD58 Antigens; D018957:CD59 Antigens; D003169:Complement Inactivator Proteins; D008562:Membrane Glycoproteins