Lipofection of synthetic oligodeoxyribonucleotide having a palindromic sequence of AACGTT to murine splenocytes enhances interferon production and natural killer activity.
A synthetic 22-mer oligodeoxyribonucleotide having an AACGTT palindrome, AAC-22, induced interferon (IFN) production and augmented the natural killer (NK) activity in murine splenocytes, whereas its analogue, ACC-22, having an ACCGGT palindrome, did not. The binding of AAC-22 to splenocytes was not different from that of ACC-22. Lipofection of AAC-22 to splenocytes remarkably enhanced IFN production and NK cell activity, whereas that of ACC-22 caused little enhancement. These results strongly suggest that the prerequisite for IFN production is not the binding of AAC-22 to the cell surface receptors, but its penetration into the spleen cells.
Yamamoto
T
T
Department of Bacterial and Blood Products, National Institute of Health, Tokyo, Japan.
Yamamoto
S
S
Kataoka
T
T
Tokunaga
T
T
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004247:DNA / Q000276:immunology*; D005260:Female; D007372:Interferons / Q000096:biosynthesis*; D007694:Killer Cells, Natural / Q000276:immunology*; D008081:Liposomes; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000276:immunology*; D013154:Spleen / Q000276:immunology*
D008081:Liposomes; D009838:Oligodeoxyribonucleotides; D004247:DNA; D007372:Interferons