Labelling of polysaccharides using [11C]cyanogen bromide. In vivo and in vitro evaluation of 11C-hyaluronan uptake kinetics.
A method for the 11C-labelling of polysaccharides in high specific radioactivity is described. Dextran and hyaluronan were treated with [11C]cyanogen bromide in aqueous solution at pH 11.5 to give 30-47% radiochemical yields with higher than 98% radiochemical purity in synthesis times of 24-26 min counted from the end of bombardment. Specific radioactivities at the end of synthesis ranged from 0.12 to 3.1 Ci/mumol. The biodistribution kinetics of [11C]hyaluronan injected intravenously was studied in rats by means of positron emission tomography, showing a rapid and displaceable uptake in liver. Uptake and displacement of [11C]hyaluronan was also demonstrated in cultured rat liver endothelial cells.
Westerberg
G
G
Uppsala University PET Centre, Sweden.
Bergström
M
M
Gustafson
S
S
Lindqvist
U
U
Sundin
A
A
Långström
B
B
D000818:Animals; D001692:Biological Transport; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics*; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D003488:Cyanogen Bromide; D003911:Dextrans / Q000737:chemistry / Q000493:pharmacokinetics*; D006820:Hyaluronic Acid / Q000737:chemistry / Q000493:pharmacokinetics*; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D008099:Liver / Q000000981:diagnostic imaging / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014055:Tomography, Emission-Computed / Q000379:methods
D002250:Carbon Radioisotopes; D003911:Dextrans; D007202:Indicators and Reagents; D006820:Hyaluronic Acid; D003488:Cyanogen Bromide