Intracellular injection of inositol 1,4,5-trisphosphate increases a conductance in membranes of turtle vomeronasal receptor neurons in the slice preparation.
Inositol 1,4,5-trisphosphate (IP3) was injected into turtle vomeronasal receptor neurons in the slice preparation under a whole-cell patch clamp, and the evoked current was measured. Application of 0.1 mM IP3 evoked a prolonged, inward current (52 of 98 neurons) with an average peak amplitude of 89.9 +/- 10.9 pA. The reversal potential of the response induced by IP3 was estimated to be -32.3 +/- 1.5 mV (6 neurons). Bathing the neurons in 10 microM ruthenium red solution greatly reduced the IP3 evoked inward current to 18.0 +/- 4.6 pA (5 neurons). This is the first study to demonstrate that the membranes of the turtle vomeronasal neurons carry IP3-activated conductance.
Taniguchi
M
M
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Kashiwayanagi
M
M
Kurihara
K
K
D000818:Animals; D002628:Chemoreceptor Cells / Q000187:drug effects* / Q000502:physiology; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009300:Nasal Septum / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D012430:Ruthenium Red / Q000494:pharmacology; D014426:Turtles
D012430:Ruthenium Red; D015544:Inositol 1,4,5-Trisphosphate