Evaluation of a simplified HEp-2 cell adherence assay for Escherichia coli isolated from south Indian children with acute diarrhea and controls.
A simplified HEp-2 cell adhesion assay was performed with stored Escherichia coli isolates from 761 children with acute diarrhea and 531 matched controls, and the results were evaluated by means of fluorescent actin staining and hybridization with DNA probes. The prevalence rates of localized adherence and aggregative adherence were significantly higher for patients (9.9 and 7.6%, respectively) than for controls (3.7 and 3.9%, respectively).
Kang
G
G
Department of Gastrointestinal Sciences, Christian Medical College Hospital, Vellore, India.
Mathan
M M
MM
Mathan
V I
VI
D000208:Acute Disease; D001422:Bacterial Adhesion; D001431:Bacteriological Techniques; D016022:Case-Control Studies; D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000302:isolation & purification* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology*; D005069:Evaluation Studies as Topic; D006801:Humans; D007194:India