title: Mechanistic understanding of enamel mineralization under fluoride regime.
abstract: In order to learn more about how the microenvironment for enamel mineralization is modified by fluoride at low concentrations (0 through 1 ppm) and how excess fluoride retards the degradation and removal of amelogenins, we studied precipitation reactions in an in vitro model utilizing a dialysis chamber. The results showed that, with the limited supply of Ca ions through the ultrafiltration membrane, the solution composition surrounding the seed crystals showed a proximity to the steady-state condition after 12-24 h equilibration. Major findings were that (a) fluoride overcame partially the inhibition of precipitation and growth reactions by enamel proteins and (b), with this accelerating effect of fluoride, the steady-state Ca concentrations in the media surrounding the seed crystals decreased substantially as a function of fluoride concentration. The overall results support the concept that the presence of fluoride in the mineralizing milieu can modify markedly the steady-state concentrations of mineral lattice ions, particularly decreasing free Ca2+ concentrations, which in turn may modulate protease activities in situ.
authors.affiliation: Department of Pathology, Nippon Dental University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Aoba
authors.forename: Y
authors.initials: Y
authors.lastname: Taya
authors.forename: A
authors.initials: A
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Shimada
authors.forename: M J
authors.initials: MJ
authors.lastname: Mura-Galelli
mesh_terms: D053523:Amelogenin; D000818:Animals; D002118:Calcium / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002627:Chemistry, Physical; D003461:Crystallography; D003743:Dental Enamel / Q000737:chemistry / Q000187:drug effects*; D003746:Dental Enamel Proteins / Q000737:chemistry / Q000187:drug effects; D003956:Dialysis / Q000295:instrumentation; D010450:Endopeptidases / Q000737:chemistry / Q000187:drug effects; D005459:Fluorides / Q000008:administration & dosage / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D008903:Minerals / Q000737:chemistry; D010758:Phosphorus / Q000032:analysis / Q000737:chemistry; D013552:Swine; D014074:Tooth Calcification / Q000187:drug effects*; D014462:Ultrafiltration / Q000295:instrumentation
chemical_list: D053523:Amelogenin; D003746:Dental Enamel Proteins; D008903:Minerals; D010758:Phosphorus; D010450:Endopeptidases; D005459:Fluorides; D002118:Calcium