Immunological study of anti-M2 in antimitochondrial antibody-negative primary biliary cirrhosis.
We investigated the specificity of various autoantibodies in antimitochondrial antibody (AMA)-negative patients with primary biliary cirrhosis (PBC). We examined sera from 144 patients with PBC, 17 of whom were AMA negative by indirect immunofluorescence. The AMA-negative group showed a significantly higher positivity for smooth muscle antibody, but not for antinuclear antibody, as compared with the AMA-positive group. IgG class anti-PDH by enzyme-linked immunosorbent assay (ELISA) were detected in 13% of the AMA-negative group. However, all PBC patients showed positive IgG, IgA, and/or IgM class anti-M2 to the four M2 proteins by immunoblotting. These results suggest that the detection of IgG and IgM class anti-PDH and that of antibodies to the four M2 proteins increases the positivity of this ELISA method, and that detection of IgG, IgA, and IgM class anti-M2 to the four M2 proteins by immunoblotting is useful in diagnosing AMA-negative patients with PBC.
Kitami
N
N
Department of Gastroenterology, Juntendo University School of Medicine, Tokyo.
Komada
T
T
Ishii
H
H
Shimizu
H
H
Adachi
H
H
Yamaguchi
Y
Y
Kitamura
T
T
Oide
H
H
Miyazaki
A
A
Ishikawa
M
M
D000328:Adult; D000368:Aged; D017153:Antibodies, Anticardiolipin / Q000097:blood; D000974:Antibodies, Antinuclear / Q000097:blood; D001323:Autoantibodies / Q000097:blood*; D064211:Connectin; D005260:Female; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D008105:Liver Cirrhosis, Biliary / Q000175:diagnosis / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000276:immunology; D009124:Muscle Proteins / Q000097:blood; D009194:Myeloma Proteins; D011768:Pyruvate Dehydrogenase Complex / Q000097:blood; D012680:Sensitivity and Specificity
D017153:Antibodies, Anticardiolipin; D000974:Antibodies, Antinuclear; D001323:Autoantibodies; D064211:Connectin; D007136:Immunoglobulins; D009124:Muscle Proteins; D009194:Myeloma Proteins; D011768:Pyruvate Dehydrogenase Complex; C085016:multiple myeloma M-proteins