Responses of immunocompetent cells to cavity preparation in rat molars: an immunohistochemical study using OX6-monoclonal antibody.
Responses of immunocompetent cells, especially class II major histocompatibility complex (MHC) antigen-expressing cells, were investigated after cavity preparation in the erupted upper first molar teeth of rats, by immunohistochemistry using OX6-monoclonal antibody. In control teeth, OX6-immunopositive cells were predominantly located beneath the odontoblast layer in the dental pulp. Cavity preparation caused an acute edematous reaction between the injured odontoblasts and predentin, and most of OX6-immunopositive cells in the affected site shifted away from the pulp-dentin border. After 12-24 hours, many OX6-immunopositive cells accumulated along the pulp-dentin border and extended their cytoplasmic processes into the exposed dentinal tubules. After 72 hours, newly differentiated odontoblasts replaced the degenerated odontoblasts, and few OX6-immunopositive cells remained along the pulp-dentin border. Our data suggest that some of the class II MHC antigen-expressing cells in the dental pulp participate in the initial defense reaction and presumably serve as a biological sensor for the external stimuli arriving through the exposed dentinal tubules.
Ohshima
H
H
Second Department of Oral Anatomy, Niigata University School of Dentistry, Japan.
Sato
O
O
Kawahara
I
I
Maeda
T
T
Takano
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology*; D002454:Cell Differentiation; D003593:Cytoplasm / Q000648:ultrastructure; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology; D003737:Dental Cavity Preparation; D003782:Dental Pulp / Q000276:immunology / Q000473:pathology; D003788:Dental Pulp Diseases / Q000276:immunology / Q000473:pathology; D003804:Dentin / Q000276:immunology / Q000473:pathology; D004487:Edema / Q000276:immunology / Q000473:pathology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D007150:Immunohistochemistry; D008963:Molar; D009804:Odontoblasts / Q000276:immunology / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II