title: The effect of peroxides on the vascular endothelium of isolated pig aorta in vitro.
abstract: The effect of peroxide on endothelial cells (perfused pig aorta) was examined using an in vitro perfusion model. Hydrogen peroxide was added to the perfusion medium (pig serum together with a buffer solution) which was expected to lead to an increased oxidation of lipids and lipoproteins. Oxidation processes of this type play a decisive role in the pathogenesis and progression of arteriosclerosis. The aim of the present investigation was to demonstrate by introducing hydrogen peroxide (H2O2) in varying concentrations (0.5-1.5%), the destructive impact of peroxides on the endothelium, while these cells are believed to play a key role in the pathogenesis of arteriosclerosis. The extent of endothelial cell impairment was assessed by means of silver staining visualisation of endothelial cell borders as well as light- and scanning-electronmicroscopic investigation. It was discovered that the endothelial cells show increasing impairment after 10 h of perfusion due to the effect of peroxide (hydrogen peroxide).
authors.affiliation: Institute of Pathology, University Leipzig, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Krautschick
authors.forename: J
authors.initials: J
authors.lastname: Krugmann
authors.forename: M
authors.initials: M
authors.lastname: Neuenfeld
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000166:cytology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D006861:Hydrogen Peroxide / Q000633:toxicity*; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D010477:Perfusion / Q000379:methods; D016622:Silver Staining / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D013552:Swine
chemical_list: D006861:Hydrogen Peroxide