title: Beta 1 integrins in third trimester human placentae: no differential expression in pathological pregnancy.
abstract: Integrins are a group of cell surface receptors that play important roles in cell-cell and cell-extracellular matrix interactions. The expression of trophoblast cell surface integrin subunits changes during placental development in normal pregnancy but the functional significance is unknown. The aim of this study was to investigate the expression of beta 1 integrins and their extracellular matrix ligands in human placenta and membranes in normal and pathological pregnancy using an avidin-biotin-peroxidase technique. Expression of the beta 1 integrins was similar in all study groups. Whilst there was some heterogeneity of expression of specific integrin alpha chains this was not characteristic of defined subject groups, variations occurring within all groups. Two distinct trophoblast subpopulations were demonstrated in the chorion laeve according to differential expression of beta 1 integrins. Trophoblast immediately adjacent to maternal decidua, which expressed alpha 1 rather than alpha 2, also comprised the majority of trophoblast in the basal plate; possession of the alpha 1, alpha 3, alpha 5, alpha 6 rather than alpha 2, alpha 3, alpha 5, alpha 6 phenotype may be important in the invasive potential of trophoblast populations. The results obtained in the present study indicate that the integrin phenotypes of third trimester uteroplacental tissues are similar in normal and pathological pregnancy, including pre-eclampsia, before and after labour.
authors.affiliation: Department of Obstetrics, St James's University Hospital, Leeds, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Divers
authors.forename: J N
authors.initials: JN
authors.lastname: Bulmer
authors.forename: D
authors.initials: D
authors.lastname: Miller
authors.forename: R J
authors.initials: RJ
authors.lastname: Lilford
mesh_terms: D005109:Extracellular Matrix / Q000737:chemistry*; D005321:Extraembryonic Membranes / Q000737:chemistry*; D005260:Female; D006801:Humans; D019012:Integrin beta1; D016023:Integrins / Q000032:analysis*; D008024:Ligands; D010641:Phenotype; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000378:metabolism*; D011263:Pregnancy Trimester, Third; D012016:Reference Values
chemical_list: D019012:Integrin beta1; D016023:Integrins; D008024:Ligands