The role of ions on histamine-induced depolarization in isolated guinea pig adipocytes.
Effects of ions on histamine (Hi)-induced depolarization were studied in guinea pig adipocytes. Depolarization induced by Hi in guinea pig adipocytes was decreased by removal of K+ from the medium or pretreatment with ouabain at concentrations that showed no significant effect themselves. The decrease in membrane potentials induced by Hi was also abolished potently by replacement of Na+ by choline or pretreatment with tetrodotoxin at a concentration that caused no significant action alone. Pretreatment with monensin at a concentration lower than that eliciting the action resulted in a potentiation of Hi-induced depolarization. The depolarization induced by Hi was not affected by the presence of Ca2+ in the medium or pretreatment of the cells by diltiazem.
Kamei
C
C
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Sugimoto
Y
Y
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D002794:Choline / Q000494:pharmacology; D003470:Culture Media; D004110:Diltiazem / Q000494:pharmacology; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology*; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D008643:Mesentery / Q000166:cytology / Q000187:drug effects; D008985:Monensin / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology
D003470:Culture Media; D007473:Ion Channels; D013779:Tetrodotoxin; D010042:Ouabain; D006632:Histamine; D008985:Monensin; D012964:Sodium; D004110:Diltiazem; D002794:Choline; D011188:Potassium; D002118:Calcium