Role of syntaxin in mouse pancreatic beta cells.
The role of syntaxin 1, a protein involved in the docking of synaptic vesicles at presynaptic active zones, has been investigated in pancreatic islet cells. Using two different monoclonal antibodies we have shown that syntaxin 1 is present in the pancreatic islet cell microsomal fraction. Furthermore, functional experiments demonstrate that anti-syntaxin antibodies inhibit CA(2+)-dependent insulin secretion in permeabilized islet cells. These data indicate that syntaxin 1 is present in the pancreatic beta cell and it is likely to play a functional role in the exocytosis of secretory granules.
Martín
F
F
Department of Physiology, University of Alicante, Spain.
Moya
F
F
Gutierrez
L M
LM
Reig
J A
JA
Soria
B
B
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D002118:Calcium / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000502:physiology; D005089:Exocytosis; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008861:Microsomes / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D050827:Syntaxin 1
D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D007328:Insulin; D009419:Nerve Tissue Proteins; C494214:Stx1a protein, mouse; D050827:Syntaxin 1; D002118:Calcium