The beta-subunit of Caenorhabditis elegans avermectin receptor responds to glycine and is encoded by chromosome 1.
A full-length cDNA encoding the beta subunit of the recently described avermectin receptor was amplified from Caenorhabditis elegans mRNA. When this cDNA was injected into Xenopus oocytes a dose-dependent response to glycine was observed, together with a smaller response to 1 mM GABA. The EC50 of the glycine response was similar to that described previously for glutamate (0.38 mM). Hybridisation of the cDNA to polytene filters identified three yeast artificial chromosome clones that gave a positive signal, Y37B3, Y38E5, and Y24C9, all of which are mapped to chromosome 1. Hybridisation to a series of cosmid clones covering this area further mapped the gene encoding this subunit to the region -2,818 to -2,824.
Laughton
D L
DL
School of Biology and Biochemistry, University of Bath, England.
Wheeler
S V
SV
Lunt
G G
GG
Wolstenholme
A J
AJ
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D017173:Caenorhabditis elegans / Q000235:genetics*; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D018014:Gene Transfer Techniques; D018698:Glutamic Acid / Q000378:metabolism / Q000494:pharmacology; D005998:Glycine / Q000378:metabolism / Q000494:pharmacology*; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D009865:Oocytes / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D012194:RNA-Directed DNA Polymerase; D018009:Receptors, Glycine / Q000235:genetics*; D014981:Xenopus; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D018076:DNA, Complementary; D046911:Macromolecular Substances; D012333:RNA, Messenger; D018009:Receptors, Glycine; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D012194:RNA-Directed DNA Polymerase; D005998:Glycine