In vitro studies of conjunctival cells from eyes with and without pseudoexfoliation.
The aim of the study was to examine for possible differences between cell cultures derived from eye with and without pseudoexfoliation. In both populations, scanning electron microscopy showed flattened epithelial cells and also spindleshaped fibroblast-like cells. The presence of these cell types was further confirmed by immunohistochemical demonstration of cytokeratin and vimentin in the cultured cells. The cells maintained in vitro showed a linear increase in uptake of leucine during a 12-h period. Within this period, the leucine recovered in the TCA precipitable fraction was considerably higher than the nonbound fraction. In cultures maintained in medium with and without L-ascorbic acid, the presence of L-ascorbic acid significantly increase the uptake of leucine into TCA precipitable material, and to a similar extent in cultures from the two populations. In conclusion, cells derived from eyes with and without pseudoexfoliation material and maintained in vitro showed similar morphology, presence of intermediate filaments, as well as uptake of leucine under various culture conditions.
Halvorsen
F
F
Department of Ophthalmology, and Pathology, Ullevaal Hospital, University of Oslo, Norway.
Nicolaissen
B
B
Ringvold
A
A
Näss
O
O
D001205:Ascorbic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D003228:Conjunctiva / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D017889:Exfoliation Syndrome / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D005136:Eye Proteins / Q000096:biosynthesis; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000032:analysis; D007930:Leucine / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D014746:Vimentin / Q000032:analysis
D005136:Eye Proteins; D014746:Vimentin; D007633:Keratins; D007930:Leucine; D001205:Ascorbic Acid