Inheritance of mitochondrial DNA in sexual crosses and protoplast cell fusions in Lentinula edodes.
By using mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) as genetic markers, the modes of mitochondrial inheritance in sexual crosses and protoplast cell fusions of the higher basidiomycete Lentinula edodes were examined. All newly established dikaryons from reciprocal crosses between compatible monokaryons carrying different mtDNA RFLP phenotypes retained mtDNA genotypes from one of the monokaryons, suggesting that mitochondrial inheritance is principally uniparental. In contrast, it was shown that recombinant mtDNA genomes arose in some dikaryons obtained after protoplast cell fusion. Based on these results, a possible mechanism for mitochondrial inheritance in L. edodes is discussed.
Fukuda
M
M
Faculty of Agriculture, Shinshu University, Nagano, Japan.
Harada
Y
Y
Imahori
S
S
Fukumasa-Nakai
Y
Y
Hayashi
Y
Y
D000363:Agaricales / Q000235:genetics*; D003433:Crosses, Genetic; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D004272:DNA, Mitochondrial / Q000235:genetics* / Q000302:isolation & purification; D005819:Genetic Markers; D009693:Nucleic Acid Hybridization; D012150:Polymorphism, Restriction Fragment Length; D011523:Protoplasts
D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D005819:Genetic Markers