title: Vaccination of cattle with the N-terminus of L2 is necessary and sufficient for preventing infection by bovine papillomavirus-4.
abstract: We have previously shown that cattle vaccinated with L2, the minor structural protein of bovine papillomavirus-4 (BPV-4), do not develop alimentary papillomas upon challenge with BPV-4. Analysis of the B and T cell response in L2-vaccinated animals showed that the majority of the response was directed against the N-terminus and C-terminus of L2 with little response against the middle portion. Cattle were vaccinated with the N-terminus or the C-terminus of L2. The animals vaccinated with the N-terminus were completely protected from viral challenge, whereas the animals vaccinated with the C-terminus were not. Further analysis with synthetic overlapping peptides spanning the entire N-terminus mapped a B cell immunodominant epitope at amino acid 101-120. This epitope was recognised by all vaccinated animals.
authors.affiliation: Beatson Institute for Cancer Research, CRC Beatson Laboratories, Glasgow, United Kingdom.
authors.forename: L M
authors.initials: LM
authors.lastname: Chandrachud
authors.forename: G J
authors.initials: GJ
authors.lastname: Grindlay
authors.forename: G M
authors.initials: GM
authors.lastname: McGarvie
authors.forename: B W
authors.initials: BW
authors.lastname: O'Neil
authors.forename: E R
authors.initials: ER
authors.lastname: Wagner
authors.forename: W F
authors.initials: WF
authors.lastname: Jarrett
authors.forename: M S
authors.initials: MS
authors.lastname: Campo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D052688:Bovine papillomavirus 1 / Q000276:immunology*; D052697:Bovine papillomavirus 4; D002213:Capsid / Q000737:chemistry / Q000276:immunology*; D036022:Capsid Proteins; D002417:Cattle; D000939:Epitopes / Q000276:immunology; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010212:Papilloma / Q000276:immunology* / Q000517:prevention & control; D030361:Papillomavirus Infections / Q000276:immunology* / Q000517:prevention & control; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry; D014412:Tumor Virus Infections / Q000276:immunology* / Q000517:prevention & control; D014614:Vaccines, Synthetic / Q000276:immunology*; D015678:Viral Structural Proteins / Q000737:chemistry / Q000276:immunology; D014765:Viral Vaccines
chemical_list: D036022:Capsid Proteins; D000939:Epitopes; C085218:L2 protein, Bovine papillomavirus; D010455:Peptides; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; D014765:Viral Vaccines