title: Superoxide scavenging activity in experimental liver transplantation.
abstract: Superoxide dismutase (SOD) activity was evaluated by measuring superoxide scavenging capability with the aid of an electron spin resonance (ESR) spin trapping method in a swine orthotopic liver transplantation (OLT) model. The animals were divided into two groups, depending on the length of the survival periods: the short survival group (n = 8) survived less than 6 days and the long survival group (n = 15) 6 days or longer. SOD activity was significantly lower in the short survival group than in the long survival group after reperfusion (P < 0.01). During the period of cold preservation, a minimal change in SOD activity was noted, regardless of the length of preservation. Serum aspartate aminotransferase (AST) levels after reperfusion and serum lactate dehydrogenase (LDH) levels 1 h after reperfusion were significantly higher in the short survival group than in the long survival group (P < 0.01 and P < 0.05, respectively). The difference in polymorphonuclear leukocytes (PMN) was significantly greater in the short survival group at 1 h after reperfusion (P < 0.01). The authors conclude that superoxide scavenging activities in the graft reflect the magnitude of reperfusion injury, which can be a reliable parameter for the estimation of graft outcome.
authors.affiliation: Department of Surgery II, Nagoya University School of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Negita
authors.forename: I
authors.initials: I
authors.lastname: Yokoyama
authors.forename: S
authors.initials: S
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Yasutomi
authors.forename: H
authors.initials: H
authors.lastname: Takagi
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001706:Biopsy; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D016031:Liver Transplantation / Q000473:pathology / Q000502:physiology*; D009926:Organ Preservation; D015424:Reperfusion; D013482:Superoxide Dismutase / Q000378:metabolism*; D013552:Swine
chemical_list: D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases