Complete sequence of the gene encoding a chorionic gonadotropin-like protein from Xanthomonas maltophilia.
Our laboratory has previously reported that: (i) Xanthomonas maltophilia (Xm) produces a protein which has immunological resemblance to the beta-subunit of human chorionic gonadotropin (hCG) and (ii) possesses a high-affinity receptor which binds holo hCG, and the endogenous ligand, Xm chorionic gonadotropin (xCG), but does not bind human luteinizing hormone (hLH). We have also previously published a 492-bp partial nucleotide sequence of the gene (xcg) coding for xCG. We report herein the entire xcg sequence of 1362 bp, which codes for a 48-kDa protein. This sequence confirmed the 492-bp sequence, as well as two partial amino acid (aa) sequences which we have previously reported. The sequence has a region which is homologous to aa 56-139 of the beta-subunit of hCG, and a second region homologous to the C-terminal tail of hCG. This is the first report of a prokaryotic gene homologous to the hCG beta-subunit-encoding gene.
Grover
S
S
Department of Internal Medicine, University of Utah Health Sciences Center, Salt Lake City 84132, USA.
Woodward
S R
SR
Odell
W D
WD
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D006063:Chorionic Gonadotropin / Q000737:chemistry / Q000235:genetics*; D018997:Chorionic Gonadotropin, beta Subunit, Human; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014974:Xanthomonas / Q000235:genetics*
D001426:Bacterial Proteins; D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D010446:Peptide Fragments; C093355:chorionic gonadotropin, Xanthomonas maltophilia