title: Postselective (directed) mutagenesis of fast-growing strain of Mycobacterium vaccae as a method of creating mutants showing changed phenotypic properties.
abstract: Postselective (directed) mutagenesis was used to create mutants of M. vaccae B3805 showing changed abilities for steroid biotransformations. Three morphological classes of such mutants, differing in colony colour: yellow, white and pink, were selected. Some of them can be helpful in genetic studies of steroid biotransformation. Two mutants were also shown to be hosts for foreign DNA.
authors.affiliation: Department of Genetics of Microorganisms, University of Lódź, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Sajduda
authors.forename: J
authors.initials: J
authors.lastname: Dziadek
authors.forename: E
authors.initials: E
authors.lastname: Golańska
authors.forename: A
authors.initials: A
authors.lastname: Rumijowska
authors.forename: A
authors.initials: A
authors.lastname: Jaworski
mesh_terms: D001711:Biotransformation; D004269:DNA, Bacterial / Q000032:analysis; D007700:Kinetics; D016296:Mutagenesis; D009154:Mutation / Q000502:physiology*; D009161:Mycobacterium / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D010957:Plasmids / Q000378:metabolism; D012855:Sitosterols / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000378:metabolism; D013256:Steroids / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D012855:Sitosterols; D013256:Steroids; C025473:gamma-sitosterol; D013250:Steroid Hydroxylases; C036815:steroid 9-alpha-hydroxylase