Comparison of histamine release from human blood monocytes, lymphocytes, adenoidal and skin mast cells.
Monocytes and lymphocytes from human blood contain 0.043 +/- 0.007 and 0.053 +/- 0.014 pg histamine/cell, respectively, which can be released by a number of stimulants (A 23187, C5a, substance P, specific allergen). The release process takes 60-120 min to reach its end point, in contrast to tissue mast cells which complete the release within 1-3 min. Both, ketotifen (10(-7) - 10(-5) M) and disodium cromoglycate (10(-5) - 10(-3) M) inhibited histamine release dose dependently up to 40-45%, which might be particularly relevant during the later stages of acute allergic or pseudoallergic reactions.
Schmutzler
W
W
Institute of Pharmacology and Toxicology Medical Faculty, RWTH, Aachen, Germany.
Bolsmann
K
K
Zwadlo-Klarwasser
G
G
D000234:Adenoids / Q000166:cytology*; D000485:Allergens / Q000494:pharmacology; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D015936:Complement C5a / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D004205:Cromolyn Sodium / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000276:immunology; D007665:Ketotifen / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000166:cytology*; D013373:Substance P / Q000494:pharmacology
D000485:Allergens; D011994:Recombinant Proteins; D003208:Concanavalin A; D013373:Substance P; D000001:Calcimycin; D015936:Complement C5a; D004205:Cromolyn Sodium; D007665:Ketotifen