title: Sequence organization of the human chromosome 2q telomere.
abstract: The terminal 240 kb of a human 2q telomere region was cloned in two overlapping yeast artificial-chromosomes (YACs). This DNA contains a region of low-copy subtelomeric repeats (within 50 kb of the 2q telomere), a segment of DNA duplicated on distal 8p23 (100 kb from the 2q telomere), and a region of single-copy DNA (230 kb from the 2q telomere). Two CpG islands are present in the DNA segment duplicated on distal 8p23. RecA-assisted restriction endonuclease cleavage of genomic DNA samples revealed a potential 55 kb chromosome length polymorphism at the 2q telomere. This work provides telomeric closure of maps for human chromosome 2q, demonstrates a novel, subtelomere-specific DNA duplication, and will permit detailed molecular and cytological studies of this human telomere region.
authors.affiliation: Wistar Institute, Philadelphia, PA 19104.
authors.forename: R A
authors.initials: RA
authors.lastname: Macina
authors.forename: D G
authors.initials: DG
authors.lastname: Negorev
authors.forename: C
authors.initials: C
authors.lastname: Spais
authors.forename: L A
authors.initials: LA
authors.lastname: Ruthig
authors.forename: X L
authors.initials: XL
authors.lastname: Hu
authors.forename: H C
authors.initials: HC
authors.lastname: Riethman
mesh_terms: D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002889:Chromosomes, Human, Pair 2; D017931:DNA Primers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D016615:Telomere
chemical_list: D017931:DNA Primers