title: Nitric oxide synthase-containing neurons in sensory ganglia of the rat are susceptible to capsaicin-induced cytotoxicity.
abstract: Nitric oxide synthase in lumbar dorsal root ganglia of neonatal rat was studied by reduced nicotinamide adenine dinucleotide phosphate diaphorase and in situ hybridization histochemistry. Induction of nitric oxide synthase in neonatal capsaicin-treated rats after sciatic axotomy was compared with the axotomy-induced nitric oxide synthase increase observed in vehicle-treated littermates. In neonatal capsaicin-treated animals, the number of neurons constitutively labeled by reduced nicotinamide adenine dinucleotide phosphate diaphorase was greatly reduced as compared to vehicle-treated littermates. Nitric oxide synthase messenger RNA was not readily identified constitutively in dorsal root ganglion neurons. Seven days after sciatic transection the induction of reduced nicotinamide adenine dinucleotide phosphate diaphorase and nitric oxide synthase messenger RNA found in the vehicle-treated group was not observed in the capsaicin group. The presence of nitric oxide synthase in dorsal root ganglion neurons thus does not appear to protect against Ca(2+)-mediated capsaicin-induced cytotoxicity. However, since some nitric oxide synthase dorsal root ganglion neurons persist after the capsaicin neurotoxicity, nitric oxide synthase expression must occur in a neurochemically diverse subpopulation of small (< 1000 microns2) neurons. The capsaicin sensitivity of most nitric oxide synthase dorsal root ganglion neurons indicates that they have unmyelinated axons and are likely to be involved in nociception.
authors.affiliation: Neurobiology and Anesthesiology Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: K
authors.initials: K
authors.lastname: Ren
authors.forename: M A
authors.initials: MA
authors.lastname: Ruda
mesh_terms: D000594:Amino Acid Oxidoreductases / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D002211:Capsaicin / Q000633:toxicity*; D005260:Female; D005727:Ganglia, Spinal / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009475:Neurons, Afferent / Q000187:drug effects / Q000201:enzymology*; D019001:Nitric Oxide Synthase; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000166:cytology / Q000187:drug effects
chemical_list: D019001:Nitric Oxide Synthase; D000594:Amino Acid Oxidoreductases; D009252:NADPH Dehydrogenase; D002211:Capsaicin