A new monoclonal antibody (A78-G/A7) to the Thomsen-Friedenreich pan-tumor antigen.
A new monoclonal antibody to the Thomsen-Friedenreich (TF) antigen (or, more precisely, epitope; Gal beta 1-3GalNAc-) has been developed that is specific for both anomeric forms of this disaccharide (TF alpha and TF beta, including related structures on glycolipids), and not assay restricted. We demonstrate that this avid antibody (A78-G/A7) is well suited for immunohistochemistry on paraffin-embedded and cryosectioned tissues, immunoblotting, ELISA techniques, and hemagglutination. Immunohistochemistry on paraffin sections does not require proteolytic or microwave pretreatment. The binding characteristics of this antibody are largely independent of variations in pH (6.0-8.2) and temperature (4-37 degrees C). Immunoblotting with KG-1 (human acute myelogenous leukemia) cells revealed a series of TF-active glycoproteins with a main band at about 155 kDa. Immunoprecipitation was performed using a new technique applicable to IgM-type antibodies.
Karsten
U
U
Max Delbrück Center for Molecular Medicine, Berlin-Buch, Germany.
Butschak
G
G
Cao
Y
Y
Goletz
S
S
Hanisch
F G
FG
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry / Q000276:immunology*; D001943:Breast Neoplasms / Q000276:immunology; D002240:Carbohydrate Sequence; D002460:Cell Line; D015179:Colorectal Neoplasms / Q000276:immunology; D000939:Epitopes / Q000737:chemistry; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015295:Antigens, Tumor-Associated, Carbohydrate; D000939:Epitopes; C016467:Thomsen-Friedenreich antigen