Angiogenesis in early choroidal neovascularization secondary to age-related macular degeneration.
BACKGROUND
The morphological features of angiogenesis in early choroidal neovascularization secondary to age-related macular degeneration are yet to be fully described.


METHODS
Six eyes from five patients which on clinical and histological examination showed advanced age-related macular degeneration and early choroidal neovascularization have been studied by transmission electron microscopy.


RESULTS
Pre-existing choroidal capillaries and venules showed changes which included endothelial cell budding, pericyte enlargement, endothelial cell sprout formation and the development of intrachoroidal new vessels. In one case, an endothelial cell sprout continuous with an intrachoroidal vessel penetrated Bruch's membrane. Examination of early subretinal pigment epithelial new vessels showed them to spread between the inner layers of Bruch's membrane within the space usually occupied by the basal linear deposit and drusen. New vessel formation took place in blind pouches at the margins of new vessel networks, either in the absence of pericytes or in the presence of mainly myofibroblast-like pericytes.


CONCLUSIONS
This ultrastructural study describes two phases of new vessel growth associated with the onset of choroidal neovascularization secondary to age-related macular degeneration. The initial intrachoroidal phase appears to be a "low-turnover" form of neovascularization which may lead to new vessels penetrating Bruch's membrane. Extensive subretinal pigment epithelial neovascularization, on the other hand, results from a "high-turnover" phase of neovascularization characterized by extensive endothelial cell proliferation and migration. Pericyte phenotypic changes associated with these different phases of neovascularization appear to relate to the dynamics of angiogenesis taking place in each process.
Killingsworth
M C
MC
Electron Microscope Unit, Bankstown-Lidcombe Group Hospital, NSW Australia.
D000368:Aged; D000369:Aged, 80 and over; D016570:Bruch Membrane / Q000648:ultrastructure; D002196:Capillaries / Q000648:ultrastructure; D002829:Choroid / Q000098:blood supply* / Q000473:pathology; D004730:Endothelium, Vascular / Q000648:ultrastructure; D006801:Humans; D008268:Macular Degeneration / Q000150:complications*; D008297:Male; D009389:Neovascularization, Pathologic / Q000209:etiology / Q000473:pathology*; D010857:Pigment Epithelium of Eye / Q000648:ultrastructure; D014699:Venules / Q000648:ultrastructure