Analysis of the core and E1 envelope region sequences of a novel variant of hepatitis C virus obtained in Indonesia.
Hepatitis C virus (HCV) is currently classified into 6 major types, HCV-1 through -6, each of which can be further divided into a few subtypes, e.g., HCV-1a, -1b, -1c, etc., on the basis of sequence variation of the viral genome. The core and E1 envelope regions of HCV genome were amplified from sera of Indonesian patients using reverse transcription-polymerase chain reaction. Sequence analysis of both core and E1 regions followed by molecular evolutionary phylogenetic analysis identified a novel sequence variant of HCV-1 (Td-6). Antibodies in the serum from which Td-6 was isolated reacted only marginally to the core protein of HCV-J, a representative strain of HCV-1b, despite strong antibody response against a mixture of the core, NS3 and NS4 proteins of HCV-1a. The possible mechanism for the diminished reactivity of the antibodies in the serum to the core protein of HCV-J is discussed.
Hotta
H
H
Department of Microbiology, Kobe University School of Medicine, Hyogo, Japan.
Doi
H
H
Hayashi
T
T
Purwanta
M
M
Soemarto
W
W
Mizokami
M
M
Ohba
K
K
Homma
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral; D004797:Enzyme-Linked Immunosorbent Assay; D014644:Genetic Variation; D016679:Genome, Viral; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000382:microbiology; D006801:Humans; D007214:Indonesia; D008969:Molecular Sequence Data; D010802:Phylogeny; D014758:Viral Core Proteins / Q000235:genetics*; D014759:Viral Envelope Proteins / Q000235:genetics*
D004279:DNA, Viral; C087035:E1 protein, Hepatitis C virus; D014758:Viral Core Proteins; D014759:Viral Envelope Proteins