Urinary albumin fragments as a new clinical parameter for the early detection of diabetic nephropathy.
Urinary albumin fragments (uAF) from patients with NIDDM were analyzed as a possible factor in the early discovery of diabetic nephropathy before emergence of microalbuminuria. SDS-PAGE and immunoblot assay were employed for detection of uAF. Samples from 252 patients with NIDDM, 158 patients with non-diabetic diseases, and 48 healthy volunteers were examined; uAF were detected in 139 (55.2%), 94 (59.5%), only one (2.1%), respectively. In diabetic patients with normoalbuminuria, uAF were detected in 48 out of 159 cases (30.2%). Two years after the initial study, 3 of the 17 diabetic patients (17.6%) with normoalbuminuria and uAF showed micro- or macroalbuminuria. It was concluded that detection of uAF might be useful for the early detection of diabetic nephropathy in NIDDM.
Yagame
M
M
Department of Internal Medicine, School of Medicine, Tokai University, Isehara.
Suzuki
D
D
Jinde
K
K
Yano
N
N
Naka
R
R
Abe
Y
Y
Nomoto
Y
Y
Sakai
H
H
Suzuki
H
H
Ohashi
Y
Y
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000419:Albuminuria / Q000209:etiology / Q000652:urine*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000652:urine*; D003928:Diabetic Nephropathies / Q000150:complications / Q000175:diagnosis* / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D005500:Follow-Up Studies; D006801:Humans; D008875:Middle Aged; D010446:Peptide Fragments / Q000652:urine*; D015203:Reproducibility of Results
D010446:Peptide Fragments