Cisplatin stimulates protein tyrosine phosphorylation in macrophages.
Cisplatin [cis-dichlorodiamine platinum (II)], a potent anti-tumor compound, stimulates immune responses by activating monocyte-macrophages and other cells of the immune system. The mechanism by which cisplatin activates these cells is poorly characterized. Since protein tyrosine phosphorylation appears to be a major intracellular signalling event that mediates cellular responses, we examined whether cisplatin alters tyrosine phosphorylation in macrophages. We found that cisplatin increased tyrosine phosphorylation of several proteins in peritoneal macrophages and in P388D1 and IC-21 macrophage cell lines. Treatment of macrophages with tyrosine kinase inhibitors, genestein and lavendustin A, inhibited cisplatin-stimulated protein tyrosine phosphorylation in macrophages. Macrophages treated with cisplatin also exhibit increased fluorescence with anti-phosphotyrosine-FITC antibody. These data indicate that protein tyrosine phosphorylation plays a role in cisplatin-induced activation of macrophages.
Kumar
R
R
School of Biotechnology, Banaras Hindu University, Varanasi, India.
Shrivastava
A
A
Sodhi
A
A
D000818:Animals; D002460:Cell Line; D002945:Cisplatin / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005455:Fluorescent Antibody Technique; D019833:Genistein; D007529:Isoflavones / Q000494:pharmacology; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation / Q000502:physiology; D008264:Macrophages / Q000201:enzymology*; D017737:Macrophages, Peritoneal / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010636:Phenols / Q000494:pharmacology; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D019000:Phosphotyrosine; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D007529:Isoflavones; D008070:Lipopolysaccharides; D010636:Phenols; D010750:Phosphoproteins; D019000:Phosphotyrosine; C062898:lavendustin A; D014443:Tyrosine; D019833:Genistein; D011505:Protein-Tyrosine Kinases; D002945:Cisplatin