2'-Fluoropolynucleotide-directed reverse transcriptase reactions. Effect of homologous polynucleotides.
Several homologous polynucleotides have been tested as inhibitors on the reactions catalyzed by avian myeloblastosis virus (AMV) reverse transcriptase, in the presence of polyribonucleotides and 2'-fluorinated polynucleotides as templates. Polynucleotides differentially inhibited the reactions catalyzed by reverse transcriptase in the presence of these synthetic templates. Polyriboadenylic acid (poly(rA), poly(2'-O-methyladenylic acid) (poly(Am)), poly(2'-fluoro-2'-deoxyadenylic acid) (poly(dAfl), polyinosinic acid (poly(rI)) and polyuridylic acid poly(rU)) inhibited the polyribonucleotide-, but not the 2'-fluorinated polynucleotide-directed reverse transcriptase activity.
Juca
M B
MB
Departamento de Bioquimica, Universidade Estadual de Campinas, S. Paulo, Brasil.
Aoyama
H
H
D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005461:Fluorine / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D011061:Poly A / Q000494:pharmacology; D011069:Poly I / Q000494:pharmacology; D011072:Poly U / Q000494:pharmacology; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D011119:Polynucleotides / Q000494:pharmacology*; D011131:Polyribonucleotides / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D018894:Reverse Transcriptase Inhibitors; D013698:Templates, Genetic
D011089:Polydeoxyribonucleotides; D011119:Polynucleotides; D011131:Polyribonucleotides; D018894:Reverse Transcriptase Inhibitors; D011061:Poly A; D011069:Poly I; D011072:Poly U; D005461:Fluorine; D008345:Manganese; D004247:DNA; D012194:RNA-Directed DNA Polymerase