Antibodies against viral nonstructural proteins in response to infection with poliovirus.
Sera from recent and past cases of poliomyelitis as well as from healthy subjects vaccinated with live attenuated poliovirus, all contain antibodies to poliovirus nonstructural proteins. Reactivity of these antisera with nonstructural protein antigens present in virus-infected HeLa cells or translated from cDNA transcripts in rabbit reticulocyte lysates was sometimes sensitive to denaturation of the antigens. The finding of antibodies to nonstructural proteins of poliovirus and other viruses should have applications in the development of diagnostic assays for virus infection and may have implications for future vaccine design.
Ehrenfeld
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Department of Molecular Biology and Biochemistry, University of California, Irvine 92717.
Brown
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Jia
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Summers
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D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D002213:Capsid / Q000235:genetics / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D000939:Epitopes / Q000737:chemistry; D005260:Female; D006367:HeLa Cells / Q000821:virology; D006801:Humans; D007106:Immune Sera; D008875:Middle Aged; D009850:Oman; D011051:Poliomyelitis / Q000276:immunology*; D017955:Poliovirus / Q000276:immunology*; D011055:Poliovirus Vaccine, Oral / Q000276:immunology; D011233:Precipitin Tests; D011487:Protein Conformation; D011489:Protein Denaturation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D000939:Epitopes; D007106:Immune Sera; D011055:Poliovirus Vaccine, Oral; D011993:Recombinant Fusion Proteins; D017361:Viral Nonstructural Proteins