A cassette vector for protein engineering the lantibiotic nisin.
Expression vectors are described that make use of a plasmid-encoded nisA cassette encoding the peptide component of nisin. Specific mutations can readily be incorporated throughout the coding region of nisA. Using this vector in a nisA mutant host, three variant nisins, with dehydroalanine (Dha) residues changed to Ala residues, were generated.
Dodd
H M
HM
Department of Genetics and Microbiology, Institute of Food Research, Norwich Research Park, Colney, UK.
Horn
N
N
Gasson
M J
MJ
D000409:Alanine / Q000031:analogs & derivatives / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D009561:Nisin / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*
D009561:Nisin; C015102:dehydroalanine; D000409:Alanine