title: Variations in urinary gamma glutamyl transferase/urinary creatinine ratio in horses with or without pleuropneumonia treated with gentamicin.
abstract: The urinary GGT/urinary creatinine (uGGT/uCR) ratio was measured on Days 1, 3 and 10 in 4 adult, healthy horses; in 6 adult, healthy horses treated with gentamicin at recommended dosages and 9 adult horses treated for pleuropneumonia with gentamicin at recommended dosages. Plasma creatinine and gentamicin trough concentrations were measured on the same days. The uGGT/uCr ratio was higher in the normal horses (mean +/- s.d. 22.85 +/- 13.69) than previously reported normal values (10.5 +/- 6.8) (Adams and McClure 1985). Analysis of variance for repeated measures was used to compare the ratio in the 3 groups while controlling for the effect of time. Sick horses had a significantly higher uGGT/uCr ratio than either of the 2 groups of normal horses. Both groups of horses that were treated with gentamicin had similar percentage increases in uGGT/uCr ratio over the treatment period with the most marked increases found between treatment Days 1 and 3. The increase in uGGT/Cr ratio was predominantly a result of an increase in uGGT activity rather than a decrease in uCr concentration. The increase in uGGT activity and uGGT/uCr ratio occurred without abnormalities in serum creatinine or gentamicin trough concentrations. These findings demonstrate that urine GGT activity and uGGT/uCr ratio should be expected to increase in response to gentamicin therapy at recommended dosages without measurable changes in serum creatinine. This suggests that an elevation of the uGGT/uCr ratio in horses being treated with gentamicin would not necessarily require changes in, or withdrawal of, the gentamicin treatment as long as increases in the plasma creatinine do not exceed 0.3 g/l and gentamicin trough concentrations are < 2 micrograms/l.
authors.affiliation: Department of Clinical Studies, New Bolton Center, University of Pennsylvania, School of Veterinary Medicine, Kennett Square 19348, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Rossier
authors.forename: T J
authors.initials: TJ
authors.lastname: Divers
authors.forename: R W
authors.initials: RW
authors.lastname: Sweeney
mesh_terms: D000818:Animals; D003404:Creatinine / Q000097:blood / Q000652:urine*; D004305:Dose-Response Relationship, Drug; D005839:Gentamicins / Q000097:blood / Q000627:therapeutic use*; D006734:Horse Diseases / Q000188:drug therapy* / Q000652:urine*; D006736:Horses / Q000652:urine*; D011001:Pleuropneumonia / Q000188:drug therapy / Q000652:urine / Q000662:veterinary*; D005723:gamma-Glutamyltransferase / Q000652:urine*
chemical_list: D005839:Gentamicins; D003404:Creatinine; D005723:gamma-Glutamyltransferase