Mapping of a serotype specific epitope of the major capsid protein VP2 of infectious pancreatic necrosis virus.
A serotype-specific, conformational epitope of VP2 of infectious pancreatic necrosis virus (IPNV) (Jasper), has been mapped by restriction enzyme site-specific deletions of cloned viral cDNA. Subclones encoding fragments of VP2 were expressed in Escherichia coli followed by polyacrylamide gel electrophoresis and Western blot analysis. The epitope (between amino acid residues 242 and 325) reacted with monoclonal anti-VP2 antibodies that neutralized the homologous (Jasper), but not the heterologous (Sp) serotype of IPNV.
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Department of Microbiology, College of Biological Sciences, University of Guelph, Ontario, Canada.
Dobos
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D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D002213:Capsid / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology; D036022:Capsid Proteins; D000939:Epitopes / Q000032:analysis; D018061:Infectious pancreatic necrosis virus / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D015183:Restriction Mapping; D012487:Salmonidae; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D012703:Serotyping
D000911:Antibodies, Monoclonal; D036022:Capsid Proteins; D000939:Epitopes; D011993:Recombinant Fusion Proteins