title: Promoter analysis of the bovine gene for seminalplasmin.
abstract: In this study we mapped the transcriptional initiation site of the gene for seminalplasmin (SAP) by primer extension analysis, situated 125 nucleotides upstream of the translational initiation site of the SAP-specific mRNA. We showed that the TATA-box in position -30 of the SAP gene is part of a functional promoter. A 280 bp region of the 5'-flanking region exerted a strong positive effect on promoter activity. In this region we identified consensus sequences for the transcriptional control elements AP1, AP2, PEA3 and GATA.
authors.affiliation: Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Biology, Göttingen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Herbers
authors.forename: K H
authors.initials: KH
authors.lastname: Scheit
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D016384:Consensus Sequence; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D029607:Seminal Vesicle Secretory Proteins
chemical_list: D011506:Proteins; D012333:RNA, Messenger; D029607:Seminal Vesicle Secretory Proteins