Nucleic acid compartmentalization within the cell nucleus by in situ transferase-immunogold techniques.
In the present review, we report on recent results obtained by in situ transferase-immunogold techniques as to the ultrastructural distribution of DNA and RNA within the cell nucleus. Special emphasis is placed on the various nucleolar components and the various enigmatic structures of the extranucleolar region: interchromatin granules, coiled bodies, and simple nuclear bodies. These data are discussed in the light of our current understanding of the functional organization of the cell nucleus.
Thiry
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Laboratory of Cellular and Tissular Biology, University of Liège, Belgium.
D002451:Cell Compartmentation; D002460:Cell Line; D002467:Cell Nucleus / Q000737:chemistry* / Q000648:ultrastructure; D004247:DNA / Q000032:analysis* / Q000648:ultrastructure; D004253:DNA Nucleotidylexotransferase / Q000032:analysis / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D017403:In Situ Hybridization; D016253:Microscopy, Immunoelectron / Q000379:methods; D012313:RNA / Q000032:analysis* / Q000648:ultrastructure; D012316:RNA Nucleotidyltransferases / Q000032:analysis / Q000648:ultrastructure; D014407:Tumor Cells, Cultured
D012313:RNA; D004247:DNA; D012316:RNA Nucleotidyltransferases; D004253:DNA Nucleotidylexotransferase