Involvement of cholinergic systems in the deficit of place learning in Morris water maze task induced by baclofen in rats.
Effects of oxotremorine on the deficit of place learning in the Morris water maze task induced by baclofen and scopolamine were examined to determine the involvement of brain cholinergic systems in the deficit of learning induced by baclofen. Rats were given 4 training trials per day with the submerged platform at a fixed location in the maze for 4 days. On day 4, rats were required to swim in the pool without the platform after the 4th training trial (probe test). Baclofen as well as scopolamine dose-dependently increased the escape latency in the training trials. In the probe test, baclofen as well as scopolamine dose-dependently reduced the duration in the quadrant where the platform had been originally located. Increased latency in the training trials and reduced duration in the probe test induced by scopolamine were dose-dependently attenuated by oxotremorine. Increased latency and reduced duration in the baclofen-treated rats were improved by oxotremorine as well as 2-hydroxysaclofen. Baclofen but not scopolamine induced motor incoordination in the rotarod test. Oxotremorine failed to improve motor incoordination induced by baclofen. These results suggest that cholinergic systems may be involved in the deficit of place learning induced by baclofen, and that the ameliorative effects of oxotremorine may not be due to improvement of motor incoordination.
Nakagawa
Y
Y
Tsukuba Research Laboratories, Experimental Biomedical Research Inc. (Jisseiken), Ibaraki, Japan.
Ishibashi
Y
Y
Yoshii
T
T
Tagashira
E
E
D000818:Animals; D001418:Baclofen / Q000031:analogs & derivatives / Q000494:pharmacology*; D002799:Cholinergic Fibers / Q000502:physiology*; D018755:GABA Agonists / Q000494:pharmacology*; D058786:GABA-B Receptor Agonists; D008297:Male; D018782:Maze Learning / Q000187:drug effects* / Q000502:physiology; D008568:Memory / Q000187:drug effects; D018721:Muscarinic Agonists / Q000494:pharmacology; D010095:Oxotremorine / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012601:Scopolamine / Q000494:pharmacology
D018755:GABA Agonists; D058786:GABA-B Receptor Agonists; D018721:Muscarinic Agonists; D010095:Oxotremorine; D012601:Scopolamine; D001418:Baclofen; C057491:2-hydroxysaclofen