title: 2'-Fluoropolynucleotide-directed reverse transcriptase reactions. Effect of homologous polynucleotides.
abstract: Several homologous polynucleotides have been tested as inhibitors on the reactions catalyzed by avian myeloblastosis virus (AMV) reverse transcriptase, in the presence of polyribonucleotides and 2'-fluorinated polynucleotides as templates. Polynucleotides differentially inhibited the reactions catalyzed by reverse transcriptase in the presence of these synthetic templates. Polyriboadenylic acid (poly(rA), poly(2'-O-methyladenylic acid) (poly(Am)), poly(2'-fluoro-2'-deoxyadenylic acid) (poly(dAfl), polyinosinic acid (poly(rI)) and polyuridylic acid poly(rU)) inhibited the polyribonucleotide-, but not the 2'-fluorinated polynucleotide-directed reverse transcriptase activity.
authors.affiliation: Departamento de Bioquimica, Universidade Estadual de Campinas, S. Paulo, Brasil.
authors.forename: M B
authors.initials: MB
authors.lastname: Juca
authors.forename: H
authors.initials: H
authors.lastname: Aoyama
mesh_terms: D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D005461:Fluorine / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D011061:Poly A / Q000494:pharmacology; D011069:Poly I / Q000494:pharmacology; D011072:Poly U / Q000494:pharmacology; D011089:Polydeoxyribonucleotides / Q000378:metabolism*; D011119:Polynucleotides / Q000494:pharmacology*; D011131:Polyribonucleotides / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D018894:Reverse Transcriptase Inhibitors; D013698:Templates, Genetic
chemical_list: D011089:Polydeoxyribonucleotides; D011119:Polynucleotides; D011131:Polyribonucleotides; D018894:Reverse Transcriptase Inhibitors; D011061:Poly A; D011069:Poly I; D011072:Poly U; D005461:Fluorine; D008345:Manganese; D004247:DNA; D012194:RNA-Directed DNA Polymerase