title: A sapecin homologue of Holotrichia diomphalia: purification, sequencing and determination of disulfide pairs.
abstract: We purified and characterized a sapecin homologue, named holotricin 1, from the hemolymph of immunized larvae of a coleopteran insect, Holotrichia diomphalia. We determined its complete amino acid sequence and three disulfide pairs. Holotricin 1 consisted of 43 amino acid residues and showed potent antibacterial activity against gram-positive bacteria, but antibacterial activity against gram-negative bacteria was not obvious.
authors.affiliation: College of Pharmacy, Pusan National University, Kum Jeon Ku, Pusan, Korea.
authors.forename: S Y
authors.initials: SY
authors.lastname: Lee
authors.forename: H J
authors.initials: HJ
authors.lastname: Moon
authors.forename: S
authors.initials: S
authors.lastname: Kawabata
authors.forename: S
authors.initials: S
authors.lastname: Kurata
authors.forename: S
authors.initials: S
authors.lastname: Natori
authors.forename: B L
authors.initials: BL
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001517:Coleoptera / Q000737:chemistry*; D004220:Disulfides / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D006458:Hemolymph / Q000737:chemistry; D007301:Insect Hormones / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D019476:Insect Proteins; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D004220:Disulfides; D007301:Insect Hormones; D019476:Insect Proteins; C095917:holotricin 1 protein, Holotrichia diomphalia; C057638:sapecin protein, Sarcophaga