title: Changes in tissue distribution of rat alpha 1-macroglobulin and pregnancy-associated alpha 1-glycoprotein after inflammatory injury.
abstract: Antiserum against rat alpha 1-macroglobulin (alpha 1MG) was produced in rabbits. Antiserum against rat pregnancy-associated alpha 1-glycoprotein (PAG) was obtained by immunization with a partly purified PAG preparation and absorption of the serum with male rat serum. Acute inflammation was produced in non-pregnant female rats by a single intramuscular injection of turpentine. The concentrations of both macroglobulins in the serum and in tissue extracts were measured by rocket immunoelectrophoresis at various times up to 7 days after injury. Inflammation produced in the rats resulted in moderately elevated serum levels of these proteins soon after injury. At first, alpha 1MG levels in a number of tissues (heart, lung, kidney, spleen, pancreas, uterus and ovary) were depressed markedly; they then stabilized. The elevated serum concentrations of alpha 1MG remained unchanged during inflammation. The store of PAG in the tissues was rapidly depleted and its serum level decreased to a normal value 7 days after injury. Our findings indicate that alpha 1MG plays a more important role in maintenance of the proteinase inhibitory potential in the rat than does PAG.
authors.affiliation: Central Research Laboratory, Postgraduate Physician Training Institute, Novokuznetsk, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Zorin
authors.forename: S G
authors.initials: SG
authors.lastname: Zhabin
authors.forename: T I
authors.initials: TI
authors.lastname: Belogorlova
authors.forename: T S
authors.initials: TS
authors.lastname: Chirikova
authors.forename: N A
authors.initials: NA
authors.lastname: Krayushkina
authors.forename: O F
authors.initials: OF
authors.lastname: Lykova
mesh_terms: D000210:Acute-Phase Reaction / Q000097:blood / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000097:blood / Q000378:metabolism*; D002846:Chromatography, Affinity; D005260:Female; D007106:Immune Sera; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D011257:Pregnancy Proteins / Q000097:blood / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution; D014425:Turpentine; D000511:alpha-Macroglobulins / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D007106:Immune Sera; D011257:Pregnancy Proteins; D000511:alpha-Macroglobulins; C489170:pregnancy-associated alpha 1-glycoprotein, rat; D014425:Turpentine