title: A study on lymphocyte activation in maedi-visna virus induced pneumonia.
abstract: The stage of activation of bronchoalveolar lavage fluid (BALF) lymphocytes and peripheral blood lymphocytes (PBL) from maedi-visna virus (MVV) infected (n = 7) and control (n = 7) sheep was investigated by assessing four parameters of lymphocyte activation; lymphocyte size and complexity, loss of CD5+ T cells, expression of cell surface interleukin-2 receptor (IL-2R) and expression of DR and DQ MHC Class II molecules. BALF lymphocytes from MVV-infected animals had a significant loss of CD5+ lymphocytes (P < 0.05) and upregulation of DR and DQ MHC Class II molecules compared with controls, consistent with BALF lymphocyte activation. No changes in cell size and complexity or expression of IL-2R were observed. No evidence of PBL activation was detected. These findings suggest an impaired BALF lymphocyte activation during MVV infection.
authors.affiliation: Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, UK.
authors.forename: I
authors.initials: I
authors.lastname: Begara
authors.forename: L
authors.initials: L
authors.lastname: Lujan
authors.forename: J
authors.initials: J
authors.lastname: Hopkins
authors.forename: D D
authors.initials: DD
authors.lastname: Collie
authors.forename: H R
authors.initials: HR
authors.lastname: Miller
authors.forename: N J
authors.initials: NJ
authors.lastname: Watt
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D018956:CD5 Antigens; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D006683:HLA-DQ Antigens / Q000276:immunology; D006684:HLA-DR Antigens / Q000276:immunology; D016130:Immunophenotyping / Q000662:veterinary; D008213:Lymphocyte Activation; D018655:Lymphocyte Count / Q000662:veterinary; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000276:immunology; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*; D015854:Up-Regulation; D014790:Visna-maedi virus / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018956:CD5 Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D015375:Receptors, Interleukin-2