[An endogenous inhibitor of monoamine oxidase A (tribulin A) from brain: purification and structure identification].
The endogenous monoamine oxidase inhibitor, tribulin, contains several components which selectively (or nonselectively) inhibit monoamine oxidases A and B. The pig brain tribulin component selectively inhibiting monoamine oxidase A was purified and identified as 4-hydroxyphenylethanol using gas chromatography-mass spectrometry. This compound was also found in the rabbit brain tribulin fraction which selectively inhibits monoamine oxidase A but has no influence on monoamine oxidase B. 4-Hydroxyphenylethanol inhibits monoamine oxidase A in an incompetitive manner with respect to the substrate, serotonin (Ki = 1.4 mM). Possible pathways of 4-hydroxyphenylethanol synthesis and its biological importance as the monoamine oxidase A inhibiting component of tribulin are discussed.
Medvedev
A E
AE
B. Baron Memorial Laboratories, Department of Chemical Pathology, Queen Charlotte and Chelsea Hospial, London, U.K.
Kamyshanskaia
N S
NS
Halket
J
J
Glover
V
V
Sandler
A
A
D000818:Animals; D001923:Brain Chemistry; D002850:Chromatography, Gel; D008401:Gas Chromatography-Mass Spectrometry; D007510:Isatin; D015394:Molecular Structure; D008996:Monoamine Oxidase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D011817:Rabbits; D012701:Serotonin / Q000378:metabolism; D013379:Substrate Specificity; D013552:Swine
D008996:Monoamine Oxidase Inhibitors; C011867:4-hydroxyphenylethanol; D012701:Serotonin; D007510:Isatin; C039776:tribulin; D010626:Phenylethyl Alcohol