Analysis of monoclonal antibodies reactive with the porcine CD4 antigen.
As result of the First International Swine CD Workshop, four monoclonal antibodies (mAb) (#002, 054, 118, and 127) clustered closely to the internal standard anti-porcine CD4 mAb, 74-12-4. To further characterize the relationship between these mAb, they were used in flow cytometry to inhibit binding of 74-12-4 to porcine lymphocytes. mAb #002 (74-12-4) and #054 (PT90) completely inhibited, while mAb #127 (10.2H2) and #118 (b38c6) partially inhibited (57% and 77% respectively), the binding of 74-12-4. Furthermore, none of the mAbs bound to a 74-12-4 negative strain of pigs. We conclude that the four mAb bind to the same, or a closely related, epitope on porcine lymphocytes.
Pescovitz
M D
MD
Indiana University, Department of Surgery and Microbiology/Immunology, Indianapolis 46202.
Aasted
B
B
Canals
A
A
Dominguez
J
J
Vizcaino
J S
JS
Pospisil
R
R
Trebichavsky
I
I
Salmon
H
H
Valpotic
I
I
Davis
W C
WC
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D001667:Binding, Competitive / Q000276:immunology; D015704:CD4 Antigens / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D005434:Flow Cytometry / Q000662:veterinary; D013552:Swine / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D000939:Epitopes