Idiotypic replica of a Toxocara canis excretory/secretory antigen epitope.
This study describes the production, characterization and use of an anti-idiotype serum raised against the monoclonal antibody TC-1 which recognizes a T. canis excretory/secretory antigen (ES Ag) epitope. Anti-idiotypic (anti-Id or Ab2) antibodies were produced in rabbits using TC-1 F(ab')2 fragments; these anti-Id inhibited ES Ag binding to biotinylated TC-1, and also inhibited a larval microprecipitation assay using TC-1. Assays show that the Ab2 beta or "internal image" of a T. canis ES Ag epitope was obtained. The antibodies have been used as an idiotypic copy of ES Ag in a diagnostic ELISA for murine toxocariosis. Affinity-purified anti-Id antibodies were used to raise a homologous anti-anti-Id (Ab3) response in rabbits. Antibody formation was followed in the sera of BALB/c mice inoculated with embryonated eggs of T. canis during a 12-month infestation. A 3-week latency period was observed before specific anti-TC-1 epitope antibodies were detected. High levels were reached at 7 weeks post-inoculation with a maximum at the ninth month, and were then maintained until the end of the experiment. The results show the possible utility of anti-Id antibodies as an ES Ag molecular replica.
Bardon
R
R
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Spain.
Guillen
J L
JL
Aguila
C
C
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000947:Antigens, Helminth / Q000276:immunology*; D001667:Binding, Competitive; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000276:immunology; D005260:Female; D015801:Helminth Proteins; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D051379:Mice; D008807:Mice, Inbred BALB C; D010063:Ovum / Q000276:immunology; D011817:Rabbits; D017166:Toxocara canis / Q000276:immunology*; D014120:Toxocariasis / Q000175:diagnosis / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D000939:Epitopes; D015801:Helminth Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; C045159:TES-26 protein, Toxocara canis