Determination of ajmalicine in reserpine raw materials by liquid chromatography with fluorescence detection.
A method is presented for determination of ajmalicine in reserpine raw materials by liquid chromatography (LC) with fluorescence detection. The sample is dissolved in a very small volume of chloroform, and the resulting solution is diluted with methanol. The reference solution of ajmalicine is prepared directly in methanol. For LC, a 30 cm long normal-phase column is used. The mobile phase is methanol containing a small volume of an aqueous solution of 1-pentanesulfonic acid, sodium salt. Detection is by fluorescence with excitation at 280 nm and emission at 360 nm. In 3 samples, the ajmalicine contents ranged from 1.2 to 1.9%.
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U.S. Food and Drug Administration, Philadelphia, PA 19106, USA.
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis*; D000959:Antihypertensive Agents / Q000032:analysis; D000970:Antineoplastic Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid; D012110:Reserpine / Q000032:analysis*; D046948:Secologanin Tryptamine Alkaloids; D013050:Spectrometry, Fluorescence; D015016:Yohimbine / Q000031:analogs & derivatives* / Q000032:analysis
D000894:Anti-Inflammatory Agents, Non-Steroidal; D000959:Antihypertensive Agents; D000970:Antineoplastic Agents; D046948:Secologanin Tryptamine Alkaloids; D015016:Yohimbine; C005709:raubasine; D012110:Reserpine