Recovery of heat-injured spores of Clostridium perfringens types B, C and D by lysozyme and an initiation protein.
Heat-injured spores of several strains of Clostridium perfringens types B, C and D could be partially recovered if lysozyme was included in the recovery medium. As little as 25 ng ml-1 was effective. D90 degrees C values of 1.3-2.6 were obtained with an approximate 2-3-fold increase in the presence of 1 /microgram ml-1 of lysozyme. In the absence of lysozyme, prolonged heating of spores resulted in the appearance of satellite colonies surrounding colonies of surviving spores. An initiation protein, previously reported in the case of type A strains, was also produced by type B, C and D strains. When added to the recovery medium it too promoted the recovery of spores from thermal injury though not as effectively as lysozyme.
Labbé
R G
RG
Department of Food Science, University of Massachusetts-Amherst 01003, USA.
Chang
C A
CA
D003016:Clostridium perfringens / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D006358:Hot Temperature; D009113:Muramidase / Q000494:pharmacology*; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000096:biosynthesis / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000187:drug effects / Q000502:physiology*
D009113:Muramidase; D009238:N-Acetylmuramoyl-L-alanine Amidase