title: Developmentally regulated mouse gene NK10 encodes a zinc finger repressor protein with differential DNA-binding domains.
abstract: Using oligonucleotides complementary to the conserved inter-finger region of a variety of previously described zinc finger-encoding genes, a novel mouse gene was cloned and characterized. The gene is localized on chromosome 8 and comprises five exons. Its corresponding mRNA is developmentally regulated in various tissues and includes an open reading frame encoding a protein of 72,422 daltons. It shares amino-terminal homologies with human KRAB (or FPB) boxes, and contains 13 zinc fingers of the C2-H2 type. The NK10 KRAB domains exhibit repressing activity when tested in GAL4 fusion protein assays. Cloning of putative target sequences revealed that the individual domains differentially contribute to zinc-dependent target DNA binding.
authors.affiliation: Institut für Genetik, Universität zu Köln, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Lange
authors.forename: A
authors.initials: A
authors.lastname: Christoph
authors.forename: H J
authors.initials: HJ
authors.lastname: Thiesen
authors.forename: G
authors.initials: G
authors.lastname: Vopper
authors.forename: K R
authors.initials: KR
authors.lastname: Johnson
authors.forename: L
authors.initials: L
authors.lastname: Lemaire
authors.forename: M
authors.initials: M
authors.lastname: Plomann
authors.forename: H
authors.initials: H
authors.lastname: Cremer
authors.forename: D
authors.initials: D
authors.lastname: Barthels
authors.forename: U A
authors.initials: UA
authors.lastname: Heinlein
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D005091:Exons / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012097:Repressor Proteins / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics*; D017422:Sequence Analysis, DNA; D016335:Zinc Fingers
chemical_list: D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C096823:Zfp90 protein, mouse