Down-modulation of c-kit mRNA and protein expression by erythroid differentiation factor/activin A.
We examined the effects of erythroid differentiation factor (EDF)/activin A on the expression of c-kit mRNA and protein in murine erythroleukemia (MEL) cells. EDF/activin A induced MEL cells to benzidine positive cells. Northern blot analysis showed that the c-kit mRNA expression was reduced synchronously with increase of beta-globin and uroporphyrinogen decarboxylase gene expression during EDF/activin A induced erythroid differentiation. Scatchard analysis indicated that the cell surface receptor number was reduced without change of affinity during differentiation. Our results suggest that EDF/activin A may act as a natural regulator of erythropoiesis with modulation of c-kit gene expression.
Hino
M
M
Second Department of Internal Medicine, Osaka City University, Medical School, Japan.
Nishizawa
Y
Y
Tatsumi
N
N
Tojo
A
A
Morii
H
H
D028341:Activins; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D015536:Down-Regulation; D004920:Erythropoiesis / Q000235:genetics; D005786:Gene Expression Regulation; D005914:Globins / Q000378:metabolism; D006133:Growth Substances / Q000502:physiology*; D006454:Hemoglobins / Q000378:metabolism; D007265:Inhibins / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics*; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D014575:Uroporphyrinogen Decarboxylase / Q000378:metabolism
D017931:DNA Primers; D006133:Growth Substances; D006454:Hemoglobins; D012333:RNA, Messenger; D028341:Activins; D007265:Inhibins; D005914:Globins; D019009:Proto-Oncogene Proteins c-kit; D014575:Uroporphyrinogen Decarboxylase