[Identification of spermatozoa L-selectin and two potential pellucida ligands].
At the molecular level, gamete interactions partly depend on zona pellucida-glycoproteins fucosylation. We show, by immunocytochemistry, the sialyl-lewisx and sialyl-lewisa oligosaccharides on human zonae pellucidae. These epitopes are potential ligands of cell adhesion molecules named selectins which are known to play a role in endothelium-leukocyte interactions. By immunofluorescence, we find the leukocyte selectin (L-selectin) on the spermatozoa head. Preincubation of spermatozoa with an anti-L-selectin monoclonal antibody produces a significant inhibition of zona pellucida tight binding, under hemizona assay conditions. In contrast, preincubation of the zonae pellucidae with anti-sialyl-lewisx or anti-sialyl-lewisa antibodies does not produce a significant inhibition of spermatozoa binding. Western blot analysis of spermatozoa-detergent extracts revealed a band at approximatively 90 kDa with the anti-L-selectin monoclonal antibody. This spermatozoa selectin could play a role in human spermatozoa-zona pellucida binding. Zona-ligands have yet to be precisely defined.
Lucas
H
H
INSERM U.419, Institut de biologie, Nantes, France.
Le Pendu
J
J
Harb
J
J
Moreau
A
A
Bercegeay
S
S
Barrière
P
P
D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D006801:Humans; D019041:L-Selectin / Q000302:isolation & purification*; D008024:Ligands; D008297:Male; D013077:Sperm Head / Q000737:chemistry*; D015044:Zona Pellucida / Q000737:chemistry*
D008024:Ligands; D019041:L-Selectin
Identification d'une L-sélectine spermatique et de 2 ligands pellucidaires potentiels.