Overexpression of mutant p53 and c-erbB-2 proteins and breast tumour take in mice.
We established a panel of 17 xenografts from primary human breast carcinomas. We examined which characteristics of the original tumours and the xenografts facilitate growth in animals. Tumours expressing medium or strong immunoreactivity for p53 protein had significantly (P < 0.05) higher incidence (92%) of in vivo tumour take than those showing weak or negative immunoreactivity (9.1%). No such association was observed between either c-erbB-2 or epidermal growth factor receptor (EGFR) expression in the original tumours and their in vivo tumour take. Following subcutaneous (s.c.) transplantation of original breast tumours or established xenografts, 7/17 tumours showed metastatic disease spread to distant sites (mainly lungs). This study suggests that selective growth of highly aggressive tumours occurs during in vivo propagation of malignant tumours, and these tumours will be of particular interest in evaluating various chemotherapeutic agents for breast cancer management.
Mehta
R R
RR
Department of Surgical Oncology, University of Illinois at Chicago 60612, USA.
Graves
J M
JM
Warso
M A
MA
Das Gupta
T K
TK
D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D002277:Carcinoma / Q000235:genetics / Q000473:pathology*; D003094:Collagen / Q000494:pharmacology*; D004338:Drug Combinations; D066246:ErbB Receptors / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006085:Graft Survival; D006801:Humans; D007279:Injections, Subcutaneous; D007797:Laminin / Q000494:pharmacology*; D008593:Menopause; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009368:Neoplasm Transplantation; D011509:Proteoglycans / Q000494:pharmacology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D014183:Transplantation, Heterologous; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis* / Q000235:genetics
D003094:Collagen; D004338:Drug Combinations; D066246:ErbB Receptors; D007797:Laminin; D009363:Neoplasm Proteins; D011509:Proteoglycans; D018719:Erb-b2 Receptor Tyrosine Kinases; D016159:Tumor Suppressor Protein p53; C059485:matrigel