Possible errors in assay for beta-glycosidase activity.
Cecal homogenates were assayed for the enzymes beta-glucosidase, beta-glucuronidase, and beta-galactosidase. Anaerobic incubation with the addition of excess 3,4-dichloronitrobenzene, a substrate for nitroreductase, significantly increased the detection of the beta-glycosidase enzymes' activities.
Chadwick
R W
RW
Health Effects Research Laboratory, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711, USA.
Allison
J C
JC
Talley
D L
DL
George
S E
SE
D000332:Aerobiosis; D000693:Anaerobiosis; D000818:Animals; D002432:Cecum / Q000201:enzymology; D005966:Glucuronidase / Q000032:analysis*; D008297:Male; D009578:Nitrobenzenes; D009596:Nitrophenols; D051381:Rats; D011916:Rats, Inbred F344; D001616:beta-Galactosidase / Q000032:analysis*; D001617:beta-Glucosidase / Q000032:analysis*
D009578:Nitrobenzenes; D009596:Nitrophenols; D001617:beta-Glucosidase; D001616:beta-Galactosidase; D005966:Glucuronidase; C024836:4-nitrophenol