title: Helix-loop-helix transcription factors regulate Id2 gene promoter activity.
abstract: Id-like helix-loop-helix (HLH) transcription factors are involved in the regulation of proliferation and differentiation of several cell types. We isolated 5' regulatory region of mouse Id2 gene and demonstrated that it contains several E-box clusters. These E-boxes mediate stimulatory effects of basic-HLH (bHLH) transcription factors ME1, ME2, and NSCL1 on Id2 promoter activity. Co-expression of Id2 blocks the stimulatory effect of bHLH transcription factors which suggests the presence of feedback loops in Id2 transcriptional regulation. Overexpression of NSCL1 in F9 cells blocks the downregulation of Id2 gene expression during retinoic acid induced differentiation. Our data demonstrate that bHLH transcription factors regulate Id2 gene expression.
authors.affiliation: Department of Anatomy and Neurobiology, Colorado State University, Fort Collins 80523, USA.
authors.forename: K
authors.initials: K
authors.lastname: Neuman
authors.forename: H O
authors.initials: HO
authors.lastname: Nornes
authors.forename: T
authors.initials: T
authors.lastname: Neuman
mesh_terms: D000818:Animals; D001483:Base Sequence; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D051792:Basic Helix-Loop-Helix Proteins; D002454:Cell Differentiation; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D015536:Down-Regulation; D005786:Gene Expression Regulation; D018257:Helix-Loop-Helix Motifs / Q000502:physiology*; D051797:Inhibitor of Differentiation Protein 2; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins; D051820:TCF Transcription Factors; D000073940:Transcription Factor 4; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D051797:Inhibitor of Differentiation Protein 2; D009419:Nerve Tissue Proteins; D012097:Repressor Proteins; D051820:TCF Transcription Factors; D000073940:Transcription Factor 4; D014157:Transcription Factors; C497452:Idb2 protein, mouse; C498272:Nhlh1 protein, mouse; C489532:Tcf12 protein, mouse; C090743:Tcf4 protein, mouse; C073366:TCF12 protein, human