Re-evaluating the AgNOR staining response in Triton X-100-treated liver cells by image analysis.
The response of liver cell nuclei to a variant of the AgNOR method, which includes a treatment with Triton X-100 prior to staining assumed to improve the quality of the AgNOR-positive images, was compared to that of the usual method by image analysis procedures. The objective was to determine whether Triton X-100 removes proteins from the nuclei to the point of affecting the AgNOR response. Statistically significant differences were not found in comparisons of a nuclear area/AgNOR-stained area ratio nor in the number of AgNOR-positive dots/aggregates. It was thus assumed that the Triton X-100 treatment did not remove nuclear proteins likely to affect the AgNOR response, though it induced a cleaner background and, consequently, a clearer contrast between the stained areas and the background.
Vidal
B de C
Bde C
Dept. of Cell Biology, UNICAMP, Campinas, SP, Brazil.
Mello
M L
ML
D000818:Animals; D007091:Image Processing, Computer-Assisted; D008099:Liver / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D009697:Nucleolus Organizer Region / Q000737:chemistry* / Q000187:drug effects; D017830:Octoxynol / Q000494:pharmacology*; D016622:Silver Staining / Q000379:methods
D017830:Octoxynol