title: Adenosine receptors modulate [Ca2+]i in hippocampal astrocytes in situ.
abstract: Cultured astroglia express both adenosine and ATP purinergic receptors that are coupled to increases in intracellular calcium concentration ([Ca2+]i). Currently, there is little evidence that such purinergic receptors exist on astrocytes in vivo. To address this issue, calcium-sensitive fluorescent dyes were used in conjunction with confocal microscopy and immunocytochemistry to examine the responsiveness of astrocytes in acutely isolated hippocampal slices to purinergic neuroligands. Both ATP and adenosine induced dynamic increases in astrocytic [Ca2+]i that were blocked by the adenosine receptor antagonist 8-(p-sulfophenyl)theophylline. The responses to adenosine were not blocked by tetrodotoxin, 8-cyclopentyltheophylline, 8-(3-chlorostyryl)caffeine, dipyridamole, or removal of extracellular calcium. The P2Y-selective agonist 2-methylthioadenosine triphosphate was unable to induce increases in astrocytic [Ca2+]i, whereas the P2 agonist adenosine 5'-O-(2-thiodiphosphate) induced astrocytic responses in a low percentage of astrocytes. These results indicate that the majority of hippocampal astrocytes in situ contain P1 purinergic receptors coupled to increases in [Ca2+]i, whereas a small minority appear to contain P2 purinergic receptors. Furthermore, individual hippocampal astrocytes responded to adenosine, glutamate, and depolarization with increases in [Ca2+]i. The existence of both purinergic and glutamatergic receptors on individual astrocytes in situ suggests that astrocytes in vivo are able to integrate information derived from glutamate and adenosine receptor stimulation.
authors.affiliation: Department of Pharmacology, University of North Carolina School of Medicine, Chapel Hill 27599-7365, USA.
authors.forename: J T
authors.initials: JT
authors.lastname: Porter
authors.forename: K D
authors.initials: KD
authors.lastname: McCarthy
mesh_terms: D000241:Adenosine / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism*; D018613:Microscopy, Confocal; D009994:Osmolar Concentration; D051381:Rats; D017470:Receptors, Glutamate / Q000378:metabolism; D011983:Receptors, Purinergic / Q000378:metabolism; D018047:Receptors, Purinergic P1 / Q000502:physiology*
chemical_list: D017470:Receptors, Glutamate; D011983:Receptors, Purinergic; D018047:Receptors, Purinergic P1; D000255:Adenosine Triphosphate; D000241:Adenosine; D002118:Calcium