Synthesis of 19-hydroxylated bile acids and identification of 3 alpha,7 alpha,12 alpha,19-tetrahydroxy-5 beta-cholan-24-oic acid in human neonatal urine.
The synthesis of 19-hydroxylated bile acids (3 alpha,19-dihydroxy-, 3 alpha,7 alpha,19-trihydroxy-, 3 alpha,12 alpha,19-trihydroxy- and 3 alpha,7 alpha,12 alpha,19-tetrahydroxy-5 beta-cholan-24-oic acids) was described. These synthesized 19-hydroxylated bile acids were used as standard samples for the analysis of bile acids in human urine by gas chromatography-mass spectrometry. 3 alpha,7 alpha,12 alpha,19-Tetrahydroxy-5 beta-cholan-24-oic acid was identified in neonatal urines (0.1-1.5 micrograms/ml and 1.5-7% of total bile acids).
Kurosawa
T
T
Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Japan.
Nomura
Y
Y
Mahara
R
R
Yoshimura
T
T
Kimura
A
A
Ikegawa
S
S
Tohma
M
M
D001647:Bile Acids and Salts / Q000138:chemical synthesis* / Q000652:urine; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002793:Cholic Acids / Q000652:urine*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007231:Infant, Newborn
C096561:3,7,12,19-tetrahydroxycholanoic acid; D001647:Bile Acids and Salts; D002793:Cholic Acids