A subset of patients with recurrent spontaneous abortion is deficient in transforming growth factor beta-2-producing "suppressor cells" in uterine tissue near the placental attachment site.
OBJECTIVE
To determine if patients with unexplained recurrent miscarriage have a deficiency of decidual immunosuppressor cells that produce transforming growth factor beta type 2, as has been found in mice with abortion due to rejection and/or trophoblast failure.


METHODS
Decidual biopsy specimens were taken as near to the placental attachment site as possible under ultrasound guidance from first trimester legal termination (control) patients with recurrent miscarriage and non-viable pregnancy, and from patients with sporadic missed abortion. The tissue was tested for TGF beta-2+ suppressor cells by in situ hybridization, immunohistochemistry, and analysis of supernatants.


RESULTS
TGF beta-2-related suppressor molecules similar but not identical to those identified in pregnant mice were released by decidual lymphoid cells. Fifty percent of 14 recurrent miscarriage patients showed a lack of suppressor cells and 59% were subnormal in comparison to 20 controls and 5 sporadic miscarriage patients, where 80-85% of the patients had detectable suppressor cells.


CONCLUSIONS
Suppressor cell deficiency is compatible with a role for rejection and/or trophoblast failure in some patients with recurrent miscarriage. Presence of suppressor cells in most patients with missed abortion (4/5) is compatible with an alternative cause of fetal death, similar to findings reported in genetic fetal death mice.
Lea
R G
RG
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
Underwood
J
J
Flanders
K C
KC
Hirte
H
H
Banwatt
D
D
Finotto
S
S
Ohno
I
I
Daya
S
S
Harley
C
C
Michel
M
M
D000026:Abortion, Habitual / Q000276:immunology*; D002474:Cell-Free System; D002478:Cells, Cultured; D003656:Decidua / Q000276:immunology* / Q000473:pathology*; D010064:Embryo Implantation / Q000276:immunology; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D011247:Pregnancy; D013491:Suppressor Factors, Immunologic / Q000096:biosynthesis / Q000172:deficiency; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000096:biosynthesis* / Q000172:deficiency
D013491:Suppressor Factors, Immunologic; D016212:Transforming Growth Factor beta