Cloning and bacterial expression of an esterolytic sFV.
Smith
R G
RG
IGEN Inc., Rockville, MD, USA.
Martin
M T
MT
Sanchez
R
R
Kenten
J H
JH
D000818:Animals; D001483:Base Sequence; D002384:Catalysis; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli; D004950:Esterases; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D005822:Genetic Vectors; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D007202:Indicators and Reagents; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping
D017931:DNA Primers; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D007202:Indicators and Reagents; D011994:Recombinant Proteins; D004950:Esterases