An explanation on the limited efficacy of detoxication against VX toxicity by purified specific antibodies.
Studies on the mechanism of detoxication against VX toxicity by purified rabbit anti-VX antibodies were carried out in mice. VX (25 micrograms/kg) was injected subcutaneously immediately following intravenous injection of purified anti-VX antibodies. Blood concentration of free VX declined linearly as the antibody dose increased. Free VX concentration in brain was far lower than that in blood. When the dose of purified anti-VX antibodies was 8 and 6 mg/kg, respectively, free VX concentration in brain and blood approached 0. At the same time, the cholinesterase (ChE) activity in brain increased along with increasing antibody dose, with 70% of normal control value detected when 8 mg/kg antibodies were administered. On the other hand, anti-VX antibodies were not able to protect blood ChE unless the VX dose was lower than 10 micrograms/kg. The studies suggest that the protective action of purified rabbit anti-VX antibodies is from VX antibodies combining with the free VX in blood and extravascular tissue to reduce the amount of VX entering into the brain.
Liao
W G
WG
Institute of Pharmacology and Toxicology, Beijing, People's Republic of China.
Rong
K T
KT
D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000494:pharmacology*; D000937:Antigen-Antibody Reactions; D001921:Brain / Q000201:enzymology; D001923:Brain Chemistry; D002800:Cholinesterase Inhibitors / Q000276:immunology / Q000633:toxicity*; D002802:Cholinesterases / Q000097:blood / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D007116:Immunization, Passive; D007150:Immunohistochemistry; D008099:Liver / Q000737:chemistry; D051379:Mice; D009946:Organothiophosphorus Compounds / Q000032:analysis / Q000097:blood / Q000276:immunology / Q000633:toxicity*; D011041:Poisoning / Q000628:therapy*; D011817:Rabbits
D000906:Antibodies; D002800:Cholinesterase Inhibitors; D002802:Cholinesterases; D009946:Organothiophosphorus Compounds; C009680:VX-agent