Use of double labeling and photo CD for morphometric analysis of injured skeletal muscle.
We used computer-assisted analysis of myofiber cross-sectional areas to measure skeletal muscle responses to injury and disease. We developed a simple, inexpensive method for measuring myofiber size in human muscle samples using Kodak photo compact discs (CDs) as the image source. The photo CD serves as a permanent image storage medium and provides a high-resolution image that can be used to detect small myofibers. The use of double labeling for dystrophin and desmin allowed positive identification of both degenerating and regenerating fibers in a single biopsy specimen.
Stauber
W T
WT
Department of Physiology, West Virginia University, Morgantown, USA.
Miller
G R
GR
Gibala
M J
MJ
MacDougall
J D
JD
D000911:Antibodies, Monoclonal; D001706:Biopsy; D016250:Compact Disks; D003893:Desmin / Q000032:analysis; D016189:Dystrophin / Q000032:analysis; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D018482:Muscle, Skeletal / Q000166:cytology* / Q000293:injuries / Q000473:pathology*; D009135:Muscular Diseases / Q000473:pathology; D010781:Photography / Q000379:methods; D005082:Physical Exertion
D000911:Antibodies, Monoclonal; D003893:Desmin; D016189:Dystrophin