title: Cholecystokinin-8 induces edematous pancreatitis in dogs associated with short burst of trypsinogen activation.
abstract: To study the early pathogenesis of acute edematous pancreatitis in dogs, we examined the relationship of pancreatic hyperstimulation with cholecystokinin-8 (10 micrograms/kg/hr intravenously for 6 hr) to alterations in circulating pancreatic enzymes and pancreatic morphology with special reference to trypsinogen activation. Cholecystokinin-8 infusion was associated with increases in plasma amylase, lipase, trypsin-like immunoreactivity, and plasma and urine trypsinogen activation peptide. Pancreatic parenchymal swelling and interlobular and subcapsular fluid accumulations were detected ultrasonographically within 2 hr of cholecystokinin-8. Circulating trypsin-like immunoreactivity and trypsinogen activation peptide in urine reached a peak at 2 and 4 hr, respectively, then declined despite progressive increases in circulating amylase and lipase and intrapancreatic fluid. No significant changes were observed in dogs receiving a saline infusion. This study illustrates that cholecystokinin-8 induces edematous pancreatitis in dogs that is associated with a short-lived burst of trypsinogen activation.
authors.affiliation: Department of Small Animal Medicine and Surgery, Royal Veterinary College, U.K.
authors.forename: K W
authors.initials: KW
authors.lastname: Simpson
authors.forename: N
authors.initials: N
authors.lastname: Beechey-Newman
authors.forename: C R
authors.initials: CR
authors.lastname: Lamb
authors.forename: J B
authors.initials: JB
authors.lastname: Smyth
authors.forename: G
authors.initials: G
authors.lastname: Hughes
authors.forename: K
authors.initials: K
authors.lastname: Coombe
authors.forename: N
authors.initials: N
authors.lastname: Sumar
authors.forename: J
authors.initials: J
authors.lastname: Hermon-Taylor
mesh_terms: D000208:Acute Disease; D000818:Animals; D004285:Dogs; D004487:Edema / Q000139:chemically induced* / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D004789:Enzyme Activation / Q000187:drug effects; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D009842:Oligopeptides / Q000032:analysis / Q000187:drug effects / Q000378:metabolism; D010179:Pancreas / Q000000981:diagnostic imaging / Q000201:enzymology / Q000473:pathology; D010195:Pancreatitis / Q000139:chemically induced* / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D012844:Sincalide / Q000633:toxicity*; D013997:Time Factors; D014362:Trypsinogen / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D014463:Ultrasonography
chemical_list: D009842:Oligopeptides; C080396:trypsinogen activation peptide; D014362:Trypsinogen; D012844:Sincalide