Angiotensin I converting enzyme activity in uranyl nitrate induced acute renal failure in rats.
Angiotensin I converting enzyme (ACE) was measured in urine, serum, and tissues from rats with acute renal failure (ARF) induced by a single subcutaneous injection (15 mg/kg BW) of uranyl nitrate (UN). Urine was collected daily until day 5, when rats were sacrificed by decapitation for the obtention of blood serum and tissues. Other groups of rats were sacrificed on days 1 and 2. These rats showed proteinuria and polyuria. The damage to the kidney proximal tubule was shown by (a) histological analysis at light and electron microscopy levels on days 1, 2, and 5, (b) the increase in urinary excretion of dipeptidyl aminopeptidase IV and N-acetyl-beta-D-glucosaminidase on days 1-5, and (c) the low molecular weight proteinuria pattern on day 1. In addition, the histological analysis at the ultrastructural level showed normal glomeruli appearance on days 1 and 2, but structural alterations on day 5. These data suggest that the increased urinary excretion of enzymes and proteins is a consequence of the tubular injury on days 1 and 2, and of tubular and glomerular injury on day 5. ACE activity increased in urine on days 1-5 and in serum on day 5. Tissue ACE activity increased in lung, small intestine, and adrenal glands; and remained unchanged in testis, aorta, brain, kidney, heart, and liver. Our data suggest that: (a) the increase in serum ACE may be secondary to the changes in tissue ACE activity, and (b) the urine ACE increase may be due to the kidney proximal tubule damage. This work supports the contention that an increase in urine ACE may be an indicator of injury to the proximal tubule.
Pedraza-Chaverrí
J
J
Departamento de Biologia, Facultad de Química, UNAM, México, DF, México.
Cruz
C
C
del Socorro Blancas
M
M
Hernández-Pando
R
R
Ibarra-Rubio
M E
ME
Larriva-Sahd
J
J
Tapia
E
E
D000118:Acetylglucosaminidase / Q000652:urine; D058186:Acute Kidney Injury / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D000818:Animals; D018819:Dipeptidyl Peptidase 4 / Q000652:urine; D007672:Kidney Cortex / Q000473:pathology; D007678:Kidney Glomerulus / Q000473:pathology; D007687:Kidney Tubules, Proximal / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism* / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014502:Uranyl Nitrate
D014502:Uranyl Nitrate; D000118:Acetylglucosaminidase; D018819:Dipeptidyl Peptidase 4; D007703:Peptidyl-Dipeptidase A