Identification of the respiratory syncytial virus-induced immunosuppressive factor produced by human peripheral blood mononuclear cells in vitro as interferon-alpha.
Respiratory syncytial virus (RSV) can inhibit the proliferative response of human peripheral blood mononuclear cells (PBMC) in vitro. This inhibition is mediated by an extracellular RSV-induced factor. In the present study, the factor was clearly identified as interferon (IFN)-alpha. The RSV-induced IFN-alpha bound strongly to PBMC and inhibited the anti-RSV proliferative response only when added within the first few days of stimulation. There was, however, no concomitant decrease in the production of interleukin (IL)-2 nor in the cell surface expression of CD25, CD71, and HLA-DR. Inhibition by RSV-induced IFN-alpha was unrelated to the levels of IL-1, -2, and -6 or of IFN-gamma induced by RSV in vitro or to the presence of IL-1 inhibitor, tumor necrosis factor-alpha, prostaglandin, or IL-10. Immunosuppression by IFN-alpha may significantly affect the outcome of infection and reinfection with RSV.
Preston
F M
FM
Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital, Brisbane, Australia.
Beier
P L
PL
Pope
J H
JH
D000998:Antiviral Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D016207:Cytokines / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007108:Immune Tolerance / Q000502:physiology*; D016898:Interferon-alpha / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000187:drug effects; D012136:Respiratory Syncytial Viruses / Q000187:drug effects*; D013601:T-Lymphocytes / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000998:Antiviral Agents; D016207:Cytokines; D016898:Interferon-alpha; D014409:Tumor Necrosis Factor-alpha