title: Microbiological assessment of dentin stained with a caries detector dye.
abstract: The purpose of this study was to assess microbiologically the efficacy of 1% acid red in propylene glycol dye to stain carious dentin. Thirty teeth with primary carious lesions involving dentin were chosen. Cavity preparation using the conventional visual and tactile criteria was done and the dye was applied to the prepared cavity. Dentin samples were collected, from carious dentin prior to cavity preparation, dye stained areas and unstained areas. The total colony forming units (CFU) in each sample were then assessed microbiologically. The results showed a highly significant difference in the total colony forming units in dye stained and dye unstained dentin samples. The 1% acid red dye in propylene glycol dye was found to be effective as an adjunctive aid in the diagnosis of carious dentin.
authors.affiliation: Department of Pedodontics and Preventive Children's Dentistry, A.B. Shetty Memorial Institute of Dental Sciences, Mangalore, India.
authors.forename: M A
authors.initials: MA
authors.lastname: Zacharia
authors.forename: A K
authors.initials: AK
authors.lastname: Munshi
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification; D015169:Colony Count, Microbial; D003731:Dental Caries / Q000175:diagnosis* / Q000382:microbiology; D003732:Dental Caries Activity Tests; D003804:Dentin / Q000382:microbiology* / Q000473:pathology; D006801:Humans; D011237:Predictive Value of Tests; D011409:Propylene Glycols / Q000633:toxicity; D012235:Rhodamines / Q000633:toxicity
chemical_list: C089090:Caries Detector; D011409:Propylene Glycols; D012235:Rhodamines