Purification and characterization of an endothelial cell-viability maintaining factor from fetal bovine serum.
Serum is an essential requirement for the growth and long-term survival of human endothelial cells, even in the presence of such defined elements such as polypeptide growth factors and hormones. A polypeptide from fetal bovine serum was isolated and characterized on the basis of long-term survival of human endothelial cells in serum-free culture. The endothelial cell viability maintaining factor has been purified to homogeneity by a combination of polyethylene glycol precipitation, hydroxylapatite, gel permeation and reverse-phase high performance liquid chromatography. The final purified endothelial cell viability maintaining factor has a molecular weight of 65,000 (reduced) and has been identified as bovine apolipoprotein H by amino-terminal amino acid sequence analysis and Western blot analysis. Endothelial cell viability maintaining factor improved a long-term viability of human endothelial cells at maximal concentrations of 2.5-5 micrograms protein/ml in serum-free medium.
Cai
G
G
Research Institute for the Functional Peptides, Yamagata, Japan.
Satoh
T
T
Hoshi
H
H
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002470:Cell Survival; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004727:Endothelium; D005312:Fetal Blood / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D053482:beta 2-Glycoprotein I
D016895:Culture Media, Serum-Free; D006023:Glycoproteins; D053482:beta 2-Glycoprotein I