Tethered benzophenone reagents for the synthesis of photoactivatable ligands.
A new radiolabeled, bifunctional photoaffinity cross-linking reagent, N-succinimidyl p-benzoyl-[2,3-3H2]dihydrocinnamate, has been synthesized in high yield and with high specific activity. This reagent can be used to append the benzophenone photophore to amino groups of small molecules, such as O-aminoalkylinositol polyphosphates and polypeptides. The resulting tritiated photoaffinity labels can be purified and manipulated in ambient light and can be activated at 360 nm.
Olszewski
J D
JD
Department of Chemistry, University at Stony Brook, New York 11794-3400, USA.
Dormán
G
G
Elliott
J T
JT
Hong
Y
Y
Ahern
D G
DG
Prestwich
G D
GD
D000345:Affinity Labels / Q000138:chemical synthesis* / Q000737:chemistry; D001577:Benzophenones / Q000138:chemical synthesis* / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000138:chemical synthesis* / Q000737:chemistry; D007202:Indicators and Reagents; D007295:Inositol Phosphates; D007553:Isotope Labeling; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D010455:Peptides; D010777:Photochemistry; D013056:Spectrophotometry, Ultraviolet; D013388:Succinimides / Q000138:chemical synthesis* / Q000737:chemistry; D014316:Tritium
D000345:Affinity Labels; D001577:Benzophenones; D003432:Cross-Linking Reagents; D007202:Indicators and Reagents; D007295:Inositol Phosphates; D008024:Ligands; C096731:N-succinimidyl 4-benzoyldihydrocinnamate; D010455:Peptides; D013388:Succinimides; D014316:Tritium