Nonradioactive colony hybridization assay for detection and enumeration of enterotoxigenic Clostridium perfringens in raw beef.
A DNA probe endolabeled with digoxigenin by PCR was developed to detect and enumerate enterotoxigenic Clostridium perfringens in raw beef. After 2 h of hybridization, membranes were developed by using an anti-digoxigenin-alkaline phosphatase conjugated antibody. The resulting chromogenic reaction allowed us to detect and enumerate < or = 10 CFU of C. perfringens per g.
Baez
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LA
Eastern Regional Research Center, U.S. Department of Agriculture, Philadelphia, Pennsylvania 19118, USA.
Juneja
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VK
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003016:Clostridium perfringens / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D015169:Colony Count, Microbial / Q000379:methods*; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial; D008460:Meat / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D015342:DNA Probes; D004269:DNA, Bacterial; D004768:Enterotoxins; C035598:enterotoxin, Clostridium