Determination of humanized anti-Tac in human serum by a sandwich enzyme linked immunosorbent assay.
A 'sandwich' enzyme-linked immunosorbent assay has been developed for measuring humanized anti-Tac (HAT), a humanized antibody to the IL-2 receptor on activated T cells (Tac), in human serum. The working range of this assay is 25-400 ng/ml with an overall precision of 5%. In this assay, the analyte, HAT, is sandwiched between Tac which is bound to a microtiter plate and biotinylated Tac that is conjugated to peroxidase labelled streptavidin. This assay was utilized to determine the pharmacokinetic parameters of HAT in patients with graft-versus-host disease.
Fayer
B E
BE
Hoffmann-La Roche, Inc., Nutley, NJ 07110-1199, USA.
Soni
P P
PP
Binger
M H
MH
Mould
D R
DR
Satoh
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000493:pharmacokinetics*; D001710:Biotin / Q000737:chemistry; D016026:Bone Marrow Transplantation / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006086:Graft vs Host Disease / Q000276:immunology; D006801:Humans; D051379:Mice; D015375:Receptors, Interleukin-2 / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000493:pharmacokinetics*
D000911:Antibodies, Monoclonal; D015375:Receptors, Interleukin-2; D011993:Recombinant Fusion Proteins; D001710:Biotin