title: Membrane-restricted regulation of Ca2+ release and influx in polarized epithelia.
abstract: Epithelial cells exist in a complex setting in which responses to mucosal or serosal environments are mediated by receptors expressed on specialized cellular domains, such as apical versus basolateral cell membranes. We investigated whether airway epithelia can react selectively through G-protein-coupled receptors to stimuli in the mucosal or serosal environments by measuring inositol phosphate and intracellular Ca2+ responses in polarized human nasal epithelial monolayers. We report here that unilateral ATP (10(-4) M) administration stimulated P2 purinoceptors and tapped pools of intracellular Ca2+ associated with the plasma membrane ipsilateral but not contralateral to stimulated receptors. Similarly, activation of plasma membrane Ca2+ influx by ATP was confined to the membrane ipsilateral to receptor stimulation. These findings demonstrate that polarized epithelia restrict P2 receptor-mediated responses to a single domain of the cell, reflecting membrane-specific generation and catabolism of inositol phosphates and confinement of calcium influx regulation to the membrane ipsilateral to the stimulated receptors.
authors.affiliation: Department of Medicine, University of North Carolina, Chapel Hill 27599, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Paradiso
authors.forename: S J
authors.initials: SJ
authors.lastname: Mason
authors.forename: E R
authors.initials: ER
authors.lastname: Lazarowski
authors.forename: R C
authors.initials: RC
authors.lastname: Boucher
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D016764:Cell Polarity; D002478:Cells, Cultured; D004848:Epithelium / Q000378:metabolism*; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism*; D018048:Receptors, Purinergic P2 / Q000378:metabolism
chemical_list: D007295:Inositol Phosphates; D018048:Receptors, Purinergic P2; D000255:Adenosine Triphosphate; D002118:Calcium