title: Molecular cloning of a widely expressed human homologue for the Drosophila trp gene.
abstract: The Drosophila transient receptor potential (trp) gene and its homologue, trpl, have been suggested to mediate calcium entry during the insect's phototransduction process. We isolated a human cDNA, human trp-1 (Htrp-1), encoding a polypeptide of 793 amino acids that is 37% identical (62% similar) to Drosophila trp and trpl. Northern analysis showed that the Htrp-1 transcript is approximately 5.5 kb and expressed in most human tissues, with higher amounts in ovary, testis, heart, and brain. Isolation of Htrp-1 suggests that a trp-type protein is present in mammals and should provide a useful tool in studying calcium-depletion induced calcium influx processes.
authors.affiliation: Department of Anesthesiology and Biological Chemistry, UCLA School of Medicine 90095-1778, USA.
authors.forename: X
authors.initials: X
authors.lastname: Zhu
authors.forename: P B
authors.initials: PB
authors.lastname: Chu
authors.forename: M
authors.initials: M
authors.lastname: Peyton
authors.forename: L
authors.initials: L
authors.lastname: Birnbaumer
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000737:chemistry / Q000235:genetics*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D015870:Gene Expression; D017344:Genes, Insect; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007301:Insect Hormones / Q000737:chemistry / Q000235:genetics*; D019476:Insect Proteins; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D050052:TRPC Cation Channels; D050051:Transient Receptor Potential Channels
chemical_list: D015220:Calcium Channels; D002148:Calmodulin-Binding Proteins; D018076:DNA, Complementary; D029721:Drosophila Proteins; D007301:Insect Hormones; D019476:Insect Proteins; D008565:Membrane Proteins; D050052:TRPC Cation Channels; D050051:Transient Receptor Potential Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; C063169:trp protein, Drosophila; C074192:trpl protein, Drosophila; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium