Synthesis and DNA binding properties of C3-, C12-, and C24-substituted amino-steroids derived from bile acids.
Seven new amino- and guanidino-substituted steroids have been synthesized from bile acid precursors, either deoxycholic acid or lithocholic acid. Their DNA binding properties have been examined using an ethidium displacement assay, through studies of hyperchromicity and thermal denaturation, and by circular dichroism. Comparison is made to simple aliphatic polyamines such as putrescine, 1,12-diaminododecane, spermidine, and spermine.
Hsieh
H P
HP
Department of Chemistry, State University of New York, Stony Brook 11794-3400, USA.
Muller
J G
JG
Burrows
C J
CJ
D001647:Bile Acids and Salts / Q000737:chemistry*; D002244:Carbon; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D003840:Deoxycholic Acid / Q000737:chemistry; D004996:Ethidium / Q000737:chemistry; D019791:Guanidine; D006146:Guanidines / Q000737:chemistry; D006361:Heating; D008095:Lithocholic Acid / Q000737:chemistry; D009691:Nucleic Acid Denaturation; D013256:Steroids / Q000138:chemical synthesis* / Q000737:chemistry; D013329:Structure-Activity Relationship
D001647:Bile Acids and Salts; D006146:Guanidines; D013256:Steroids; D003840:Deoxycholic Acid; D008095:Lithocholic Acid; D002244:Carbon; D004247:DNA; D004996:Ethidium; D019791:Guanidine