title: Subunit III of cytochrome c oxidase influences the conformation of subunits I and II: an infrared study.
abstract: The secondary structure of wild-type Paracoccus denitrificans cytochrome c oxidase obtained by decomposition of the infrared amide I band contains 44% alpha-helix, 18% beta-sheet, 14% beta-turns, 18% loops, and 6% nonordered segments. The mutant lacking subunit III presents a small but significant increase (from 18% to 24%) in the percentage of loops and slight differences in the other components. Using band/area ratios and tyrosine side chain absorption as an inner standard, it is shown that in the absence of subunit III the structure of subunits I and II is altered although no changes in their alpha-helix or beta-sheet content are observed. In the bacterial oxidase, thermal infrared studies show a complex denaturation pattern characterized by the presence of a partially denatured intermediate state. Of the seven predicted subunit III alpha-helices, only four are resistant toward the thermal challenge and behave as expected for typical transmembrane helices. The observation that the absence of subunit III influences the conformation of loop regions in the two other subunits suggests that part of the interaction surface between subunit III and the catalytic subunits might be located outside the lipid bilayer.
authors.affiliation: Departamento de Bioquímica, Universidad del Pais Vasco, Bilbao, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Echabe
authors.forename: T
authors.initials: T
authors.lastname: Haltia
authors.forename: E
authors.initials: E
authors.lastname: Freire
authors.forename: F M
authors.initials: FM
authors.lastname: Goñi
authors.forename: J L
authors.initials: JL
authors.lastname: Arrondo
mesh_terms: D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000235:genetics; D017353:Gene Deletion; D006358:Hot Temperature; D046911:Macromolecular Substances; D010231:Paracoccus denitrificans / Q000201:enzymology* / Q000235:genetics; D017433:Protein Structure, Secondary; D013055:Spectrophotometry, Infrared; D013816:Thermodynamics
chemical_list: D046911:Macromolecular Substances; D003576:Electron Transport Complex IV