Cloning, subcellular localization and expression of CHL1, a subunit of magnesium-chelatase in soybean.
Mg-insertion is the first committed step in chlorophyll synthesis and is catalyzed by Mg-chelatase. In photosynthetic bacteria, bchI gene product was suggested to be a subunit of Mg-chelatase. We isolated a bchI homolog from a soybean cDNA library and designated it as chlI. CHLI consisted of 421 amino acid residues and the sequence exhibited a high similarity to other BchI homologs. CHLI contained an ATP-binding motif found in other BchI homologs. CHLI was localized in the soluble fraction in soybean chloroplasts, suggesting that it was a stromal subunit of Mg-chelatase. chlI mRNA in cell culture (SB-P) of soybean was reversibly induced by light.
Nakayama
M
M
Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Masuda
T
T
Sato
N
N
Yamagata
H
H
Bowler
C
C
Ohta
H
H
Shioi
Y
Y
Takamiya
K
K
D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology; D001665:Binding Sites; D015152:Blotting, Northern; D015153:Blotting, Western; D002736:Chloroplasts / Q000201:enzymology; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000737:chemistry; D005056:Euglena gracilis / Q000201:enzymology; D015870:Gene Expression; D008190:Lyases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D016354:Rhodobacter capsulatus / Q000201:enzymology; D016415:Sequence Alignment; D013025:Glycine max / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology*
D018076:DNA, Complementary; D012333:RNA, Messenger; D000255:Adenosine Triphosphate; D008190:Lyases; C086788:magnesium chelatase