Antipyrine, coumarin and glipizide affect n-acetylation measured by caffeine test.
The effect of various treatments on acetylation status measured by caffeine metabolites was investigated in 17 subjects with non-insulin dependent diabetes mellitus (NIDDM). The test drugs, caffeine (200 mg), antipyrine (20 mg/kg) and coumarin (5 mg), were given simultaneously, and urinary 5-acetylamino-6-formyl-amino-3-methyluracil/1-methylxanthine (AFMU/1X) molar ratio was measured before and after 8 weeks of therapy. The urinary AFMU/1X molar ratio decreased (p < 0.05) after 8 weeks of therapy with glipizide (2.5 mg), but remained unaltered in those treated with placebo or those who self-monitored blood glucose (SMBG) by glucometer. Antipyrine and coumarin decreased (p < 0.05) the AFMU/1X molar ratio both in diabetics and healthy volunteers. Our data demonstrate that glipizide, antipyrine and coumarin may interfere with the classification of acetylator status measured by caffeine metabolites.
Klebovich
I
I
Department of Pharmacology and Toxicology, University of Oulu, Finland.
Rautio
A
A
Salonpää
P
P
Arvela
P
P
Pelkonen
O
O
Sotaniemi
E A
EA
D000107:Acetylation / Q000187:drug effects; D000328:Adult; D000704:Analysis of Variance; D000983:Antipyrine / Q000494:pharmacology*; D002110:Caffeine; D003374:Coumarins / Q000494:pharmacology*; D003924:Diabetes Mellitus, Type 2 / Q000652:urine*; D005260:Female; D005913:Glipizide / Q000494:pharmacology*; D006801:Humans; D007004:Hypoglycemic Agents / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D014498:Uracil / Q000031:analogs & derivatives / Q000652:urine; D014970:Xanthines / Q000652:urine
D003374:Coumarins; D007004:Hypoglycemic Agents; D014970:Xanthines; C008514:methylxanthine; D002110:Caffeine; D014498:Uracil; C036692:5-acetylamino-6-formylamino-3-methyluracil; D000983:Antipyrine; D005913:Glipizide