title: Structural model for the beta-amyloid fibril based on interstrand alignment of an antiparallel-sheet comprising a C-terminal peptide.
abstract: Amyloids are a class of noncrystalline, yet ordered, protein aggregates. A new approach was used to provide the initial structural data on an amyloid fibril--comprising a peptide (beta 34-42) from the C-terminus of the beta-amyloid protein--based on measurement of intramolecular 13C-13C distances and 13C chemical shifts by solid-state 13C NMR and individual amide absorption frequencies by isotope-edited infrared spectroscopy. Intermolecular orientation and alignment within the amyloid sheet was determined by fitting models to observed intermolecular 13C-13C couplings. Although the structural model we present is defined to relatively low resolution, it nevertheless shows a pleated antiparallel beta-sheet characterized by a specific intermolecular alignment.
authors.affiliation: Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA.
authors.forename: P T
authors.initials: PT
authors.lastname: Lansbury
authors.forename: P R
authors.initials: PR
authors.lastname: Costa
authors.forename: J M
authors.initials: JM
authors.lastname: Griffiths
authors.forename: E J
authors.initials: EJ
authors.lastname: Simon
authors.forename: M
authors.initials: M
authors.lastname: Auger
authors.forename: K J
authors.initials: KJ
authors.lastname: Halverson
authors.forename: D A
authors.initials: DA
authors.lastname: Kocisko
authors.forename: Z S
authors.initials: ZS
authors.lastname: Hendsch
authors.forename: T T
authors.initials: TT
authors.lastname: Ashburn
authors.forename: R G
authors.initials: RG
authors.lastname: Spencer
mesh_terms: D000544:Alzheimer Disease / Q000209:etiology; D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015723:Gene Library; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D017433:Protein Structure, Secondary
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments