title: Physical association and functional relationship between protein kinase C zeta and the actin cytoskeleton.
abstract: Protein kinase C (PKC) was initially identified as a serine/threonine protein kinase dependent on calcium and phospholipids and shown to be involved in intracellular signaling pathways. PKC isoforms have been classified into four groups: Ca(2+)-dependent conventional PKC alpha, beta I, beta II, gamma; Ca(2+)-independent, novel PKC delta, epsilon, eta, phi; atypical PKC zeta, lambda, iota which are not activated by Ca2+ or diacylglycerol, and the recently discovered PKCmu. We reported that activation of the zeta PKC isoform is an important step in interleukin-2 (IL-2)-mediated proliferation (Gómez, J., Pitton, C., García, A., Martínez, A., Silva, A. and Rebollo, A., Exp. Cell Res. 1995. 218: 105.). zeta PKC is also required for mitogenic activation of fibroblasts and for the maturation pathway activated by insulin and Ras. Contradictory results have been reported regarding the subcellular redistribution of zeta PKC upon activation. We report here, using confocal microscopy, that IL-2 induces expression, translocation and association of zeta PKC to a structure coincident with the actin cytoskeleton. Furthermore, we show that zeta PKC has a role in maintaining the integrity of the actin cytoskeletal structure in IL-2-stimulated cells. On the contrary, zeta PKC is not involved in the actin cytoskeleton organization when cells are maintained in IL-4, confirming our previous results showing that IL-4-induced signal transduction is PKC independent.
authors.affiliation: Centro de Investigaciones Biológicas, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Gómez
authors.forename: A
authors.initials: A
authors.lastname: Martínez de Aragón
authors.forename: P
authors.initials: P
authors.lastname: Bonay
authors.forename: C
authors.initials: C
authors.lastname: Pitton
authors.forename: A
authors.initials: A
authors.lastname: García
authors.forename: A
authors.initials: A
authors.lastname: Silva
authors.forename: M
authors.initials: M
authors.lastname: Fresno
authors.forename: F
authors.initials: F
authors.lastname: Alvarez
authors.forename: A
authors.initials: A
authors.lastname: Rebollo
mesh_terms: D000199:Actins / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure*; D007376:Interleukin-2 / Q000494:pharmacology*; D015847:Interleukin-4 / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism* / Q000648:ultrastructure; D051379:Mice; D018613:Microscopy, Confocal; D011493:Protein Kinase C / Q000378:metabolism* / Q000648:ultrastructure; D013601:T-Lymphocytes / Q000378:metabolism / Q000648:ultrastructure*; D000099221:Protein Kinase C zeta
chemical_list: D000199:Actins; D007376:Interleukin-2; D015847:Interleukin-4; D007527:Isoenzymes; D011493:Protein Kinase C; D000099221:Protein Kinase C zeta