title: Reduced expression of platelet surface glycoprotein receptor IIb/IIIa at hyperthermic temperatures.
abstract: BACKGROUND
  Hyperthermic temperatures exist from the heat dissipation of the implantable energy source of an artificial heart. This procedure as well as therapies for cancer and thermal injuries pose a new medical problem. Among many reported effects of heat on biologic systems, platelet functions such as maximal aggregation and adhesion are known to be reduced. Using flow cytometry, we have studied platelet dysfunction at elevated temperatures and have gained a mechanistic comprehension of the loss of platelet function.
  
  
  METHODS
  Platelet rich plasma was incubated at differing temperatures for 1 hour. Immediately after, the platelets were stained using mAb against glycoprotein IIb/IIIa (GPIIb-IIIa) (CD41a) and other platelet surface glycoproteins (GP) involved in aggregation and adhesion. Relative fluorescence intensity was measured using single-labeled, laser flow cytometry to determine changes in GP surface expression. In addition, scanning electron microscopy was used to evaluate morphologic changes.
  
  
  RESULTS
  Hyperthermic temperatures between 40 and 44 degrees C significantly lowered the mAb cell surface binding in vitro of GP that participate in aggregation and adhesion. The most dramatic temperature-dependent loss of mAb binding was demonstrated by anti-GPIIb-IIIa, the mAb against the fibrinogen receptor. mAb binding to this receptor at 44 degrees C was decreased to 6.2% of a base-line fluorescence intensity of 654 (arbitrary units). The ADP-induced aggregation of platelets incubated at the same temperature also decreased to 2.1% of maximum aggregation. Other mAb, such as those against the von Willebrand factor receptor (GPIb) (CD42b), the thrombospondin receptor (GPIV) (CD36), and GPIIIa (CD61), also showed statistically significant reduction of mAb binding but to a lesser degree. Finally, scanning electron microscopy as well as side-scatter density plots from flow cytometry revealed that platelets became more spherical after incubation at 44 degrees C.
  
  
  CONCLUSIONS
  The significant reduction in mAb binding correlates with functional impairment exhibited during hyperthermic incubation. Our results support the loss of binding ability of surface GP that are involved in aggregation and adhesion as a mechanism of platelet dysfunction upon heating. GPIIb-IIIa appeared the most susceptible to heat and the principal agent in thermal induced loss of platelet function. Significant morphologic changes at 44 degrees C, the critical temperature at which ADP-induced aggregation ceases, may contribute as well.
authors.affiliation: Department of Biomedical Engineering, Cleveland Clinic Foundation, Ohio, USA.
authors.forename: R
authors.initials: R
authors.lastname: Pasha
authors.forename: M
authors.initials: M
authors.lastname: Benavides
authors.forename: K
authors.initials: K
authors.lastname: Kottke-Marchant
authors.forename: H
authors.initials: H
authors.lastname: Harasaki
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D005334:Fever / Q000503:physiopathology*; D005434:Flow Cytometry; D006801:Humans; D010974:Platelet Aggregation / Q000502:physiology; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000276:immunology; D010980:Platelet Membrane Glycoproteins / Q000502:physiology*; D016024:Receptors, Cytoadhesin / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000276:immunology; D013312:Stress, Physiological / Q000503:physiopathology
chemical_list: D000911:Antibodies, Monoclonal; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D010980:Platelet Membrane Glycoproteins; D016024:Receptors, Cytoadhesin