title: Post-inoculation changes in enzyme activity of Aedes aegypti infected with Chikungunya virus.
abstract: Levels of acetylcholinesterase, non-specific esterases, glutathione-S-transferase and glucose-6-phosphate dehydrogenase in Aedes aegypti (L.) mosquitoes inoculated intrathoracally with Chikungunya virus were elevated, as compared to uninoculated control insects. A number of these enzymes are important in the insects defence mechanism against xenobiotics, such as pesticides. Malathion bioassays indicated a reduction in the susceptibility of experimentally injected insects with virus or virus-free inoculum, compared to non-inoculated controls. However, insects which were mock-inoculated (injected with no inoculum) showed a similar reduction in susceptibility suggesting that the observed effect was due to the mobilization of a defence reaction in the mosquitoes in response to injury during inoculation.
authors.affiliation: Department of Medical Entomology, National Institute of Virology, Pune, India.
authors.forename: D T
authors.initials: DT
authors.lastname: Mourya
authors.forename: J
authors.initials: J
authors.lastname: Hemingway
authors.forename: C J
authors.initials: CJ
authors.lastname: Leake
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000330:Aedes / Q000201:enzymology* / Q000821:virology; D000818:Animals; D002478:Cells, Cultured; D002646:Chikungunya virus / Q000254:growth & development; D002522:Chlorocebus aethiops; D004950:Esterases / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D008294:Malathion; D026023:Permethrin; D001074:Propoxur; D011722:Pyrethrins; D014709:Vero Cells
chemical_list: D011722:Pyrethrins; D026023:Permethrin; D001074:Propoxur; D005954:Glucosephosphate Dehydrogenase; D005982:Glutathione Transferase; D004950:Esterases; D000110:Acetylcholinesterase; D008294:Malathion