title: Efficient gene synthesis by Klenow assembly/extension-Pfu polymerase amplification (KAPPA) of overlapping oligonucleotides.
authors.affiliation: Mary Imogene Bassett Hospital (MIBH), Cooperstown, New York 13326, USA.
authors.forename: E W
authors.initials: EW
authors.lastname: Holowachuk
authors.forename: M S
authors.initials: MS
authors.lastname: Ruhoff
mesh_terms: D003001:Cloning, Molecular; D004247:DNA / Q000138:chemical synthesis*; D004256:DNA Polymerase I; D017931:DNA Primers / Q000138:chemical synthesis; D004259:DNA-Directed DNA Polymerase; D004926:Escherichia coli; D005813:Genes, Synthetic; D021141:Nucleic Acid Amplification Techniques; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D015183:Restriction Mapping
chemical_list: D017931:DNA Primers; D009838:Oligodeoxyribonucleotides; D004247:DNA; C072146:Pfu DNA polymerase; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase