A simple "universal" DNA extraction procedure using SDS and proteinase K is compatible with direct PCR amplification.
Goldenberger
D
D
Department of Medical Microbiology, University of Zürich, Switzerland.
Perschil
I
I
Ritzler
M
M
Altwegg
M
M
D003353:Corynebacterium diphtheriae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004247:DNA / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D019286:Endopeptidase K; D013293:Enterococcus faecalis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007202:Indicators and Reagents; D016133:Polymerase Chain Reaction / Q000379:methods*; D011136:Polysorbates; D012680:Sensitivity and Specificity; D012697:Serine Endopeptidases; D012967:Sodium Dodecyl Sulfate; D013211:Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D007202:Indicators and Reagents; D011136:Polysorbates; D012967:Sodium Dodecyl Sulfate; D004247:DNA; D012697:Serine Endopeptidases; D019286:Endopeptidase K