title: Estimates of the effect of feeding on whole-body protein degradation in women vary with the amino acid used as tracer.
abstract: We measured how feeding status affects the kinetics of multiple indispensable amino acids in four adult female subjects studied both in fed and fasted state. The subjects ingested one dose of uniformly 13C-labeled algae (Spirulina platensis). The isotopic enrichments (measured with negative chemical ionization gas chromatography-mass spectrometry) of the branched chain amino acids, phenylalanine, lysine and threonine were followed for 24 h in both the plasma and in VLDL-apolipoprotein B-100 (apoB-100). Fasting lowered body protein degradation when measured with the branched chain amino acids, increased it when measured with phenylalanine and had no statistically significant effect when determined from the kinetics of lysine and threonine. These apparent differences challenge the adequacy of current models of whole-body protein turnover. The ratio of the peak labeling of amino acids in plasma and apoB-100 was used as an estimate of the isotopic dilution in the hepatic pool. In contrast to our earlier observations during intravenous tracer amino acid administration, in the present study fasting lowered the ratio of the peak isotopic enrichments of apoB-100 and plasma amino acids. This supports our contention that feeding increases the use of hepatic portal amino acids for hepatic secretory protein synthesis.
authors.affiliation: USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: H K
authors.initials: HK
authors.lastname: Berthold
authors.forename: F
authors.initials: F
authors.lastname: Jahoor
authors.forename: P D
authors.initials: PD
authors.lastname: Klein
authors.forename: P J
authors.initials: PJ
authors.lastname: Reeds
mesh_terms: D000328:Adult; D000596:Amino Acids / Q000032:analysis / Q000097:blood* / Q000378:metabolism; D001055:Apolipoproteins B / Q000097:blood / Q000378:metabolism; D002247:Carbon Isotopes; D000458:Cyanobacteria; D004435:Eating / Q000502:physiology*; D005215:Fasting / Q000502:physiology; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008239:Lysine / Q000097:blood / Q000378:metabolism; D010649:Phenylalanine / Q000097:blood / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D013912:Threonine / Q000097:blood / Q000378:metabolism; D013997:Time Factors
chemical_list: D000596:Amino Acids; D001055:Apolipoproteins B; D002247:Carbon Isotopes; D011506:Proteins; D013912:Threonine; D010649:Phenylalanine; D008239:Lysine