Advances in the use of Bacillus subtilis for the expression and secretion of heterologous proteins.
During the past year, significant progress has been made using Bacillus subtilis to produce a wide range of foreign proteins. Through strain improvement and co-expression of molecular chaperones, secretory proteins can be produced at a higher level. Through protein engineering, target proteins can be redesigned to have better stability and solubility. A combination of these two strategies would be a useful approach to produce heterologous proteins from B. subtilis at high quality and with a high yield.
Wong
S L
SL
Department of Biological Science, University of Calgary, Alberta, Canada.
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D018832:Molecular Chaperones / Q000502:physiology; D015202:Protein Engineering; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000378:metabolism
D018832:Molecular Chaperones; D021382:Protein Sorting Signals; D011994:Recombinant Proteins