Cloning of the gene encoding the murine orphan receptor TAK1 and cell-type-specific expression in testis.
We have cloned the gene encoding the mouse homologue of the orphan receptor, TAK1, a member of the nuclear receptor superfamily, from a mouse testis cDNA library. The amino acid sequence of mouse TAK1 (mTAK1) is highly homologous to that of human TAK1, with an overall identity of 98%. Northern blot analysis using RNA from different testicular cell types showed that the mTAK1 transcript is predominantly expressed in pachytene spermatocytes at a low level in round spermatids, but not in germ cells at earlier phases of spermatogenesis or in Sertoli cells. Southern analysis using genomic DNA prepared from a panel of hamster/human and mouse/human hybrid cell lines indicated that the TAK1 gene is located on human chromosome 3.
Hirose
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Cell Biology Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
O'Brien
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DA
Jetten
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AM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D011987:Receptors, Steroid / Q000096:biosynthesis / Q000235:genetics*; D011988:Receptors, Thyroid Hormone / Q000096:biosynthesis / Q000235:genetics*; D013737:Testis / Q000378:metabolism*
C490515:NR2C2 protein, human; C490514:Nr2c2 protein, mouse; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone