Introduction of Pseudomonas aeruginosa mutator phage D3112 into Alcaligenes eutrophus strain CH34.
We have investigated the possibility of growing mutator phages from Pseudomonas aeruginosa on various isolates of Alcaligenes eutrophus. Although none out of 10 A. eutrophus strains were susceptible to infection with any of the phages tested, phage D3112 could be readily transferred in our model strain CH34 by means of an RP4::D3112 plasmid. CH34/RP4::D3112 lysogens were stable and produced phages. However, neither mitomycin C nor UV treatment increased the phage yield.
Krylov
V
V
Institute for Genetics of Microorganisms, Moscow.
Merlin
C
C
Toussaint
A
A
D000421:Alcaligenes / Q000187:drug effects / Q000528:radiation effects / Q000821:virology*; D004587:Electrophoresis, Agar Gel; D066298:In Vitro Techniques; D008242:Lysogeny; D016685:Mitomycin / Q000494:pharmacology; D016254:Mutagenesis, Insertional; D017105:Pseudomonas Phages / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects; D011550:Pseudomonas aeruginosa / Q000821:virology; D014466:Ultraviolet Rays
D016685:Mitomycin