title: In-vitro analyses of mechanisms of B-cell development.
abstract: B-cell lymphopoiesis in vivo is very complex due to the influences of cooperating cells, cytokines and other receptor-ligand interactions which appear to occur developmentally at different cellular stages. Therefore in-vitro models will help to unravel this complex situation. Here, we review our and others' work on in-vitro models of B-cell development. The role of stromal cells, cytokines, surrogate light chain and products of rearranged Ig-loci in the developmentally different cellular stages will be discussed.
authors.affiliation: Basel Institute for Immunology, Switzerland.
authors.forename: A
authors.initials: A
authors.lastname: Rolink
authors.forename: P
authors.initials: P
authors.lastname: Ghia
authors.forename: U
authors.initials: U
authors.lastname: Grawunder
authors.forename: D
authors.initials: D
authors.lastname: Haasner
authors.forename: H
authors.initials: H
authors.lastname: Karasuyama
authors.forename: C
authors.initials: C
authors.lastname: Kalberer
authors.forename: T
authors.initials: T
authors.lastname: Winkler
authors.forename: F
authors.initials: F
authors.lastname: Melchers
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D002454:Cell Differentiation / Q000276:immunology*; D002478:Cells, Cultured; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D051379:Mice; D018448:Models, Immunological