[Inheritable phenotypic normalization of rodent cells transformed by simian adenovirus SA7 E1 oncogenes by singled-stranded oligonucleotides complementary to a long region of integrated oncogenes].
G11 mouse cells and SH2 rat cells transformed with simian adenovirus SA7 DNA showed inheritable oncogen-specific phenotypic normalization when treated with sense and antisense oligonucleotides complementary to long RNA sequences, plus or minus strands of the integrated adenovirus oncogenes E1A and E1B. Transitory treatment of the cells with the oligonucleotides in the absence of serum was shown to cause the appearance of normalized cell lines with fibroblastlike morphology, slower cell proliferation, and lack of ability to form colonies in soft agar. Proliferative activity and adhesion of the normalized cells that established cell lines were found to depend on the concentration of growth factors in the cultural medium. In some of the cell lines, an inhibition of transcription of the E1 oncogenes was observed. The normalization also produced cells that divided 2 - 5 times and died and cells that reverted to a transformed phenotype in 2 - 10 days. The latter appeared predominantly upon the action of the antisense oligonucleotides.
Grineva
N I
NI
Hematology Research Center, Russian Academy of Medical Sciences, Moscow 125167 Russia.
Borovkova
T V
TV
Sats
N V
NV
Kurabekova
R M
RM
Rozhitskaia
O S
OS
Solov'ev
G Ia
GIa
Pantin
V I
VI
D016475:3T3 Cells; D017498:Adenovirus E1 Proteins / Q000235:genetics*; D000261:Adenoviruses, Simian / Q000235:genetics*; D000818:Animals; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000235:genetics; D018076:DNA, Complementary; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D051379:Mice; D009841:Oligonucleotides / Q000494:pharmacology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D009857:Oncogenes; D010641:Phenotype; D051381:Rats
D017498:Adenovirus E1 Proteins; D018076:DNA, Complementary; D009841:Oligonucleotides; D016376:Oligonucleotides, Antisense
Nasleduemaia fenotipicheskaia normalizatsiia transformirovannykh E1 onkogenami adenovirusa obez'ian SA7 kletok gryzunov odnotiazhevymi oligonukleotidami, komplementarnymi protiazhennym uchastkam integrirovannykh onkogenov.