Effects of C-terminal parathyroid hormone-related peptide on osteoblasts.
A C-terminal analog of parathyroid hormone-related peptide (PTHrP), PTHrP(107-139), was found to stimulate cAMP production in three osteoblast cell preparations. The effect was studied most extensively in ROS 17/2.8 cells. The effect was dose-related and comparable in magnitude to that produced by PTHrP(1-34), but potency was lower. The functional significance of the cAMP effect is unknown, but preliminary findings indicated that PTHrP(107-139) also inhibited osteopontin mRNA levels in ROS 17/2.8 cells treated with peptide for 48 h. The results suggest that the carboxy-terminal region of PTHrP may play a role in bone metabolism by influencing osteoblast activity.
Seitz
P K
PK
Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston 77555-1031, USA.
Zhang
R W
RW
Simmons
D J
DJ
Cooper
C W
CW
D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D015290:Second Messenger Systems / Q000187:drug effects; D013268:Stimulation, Chemical; D014407:Tumor Cells, Cultured
D010281:Parathyroid Hormone; D044162:Parathyroid Hormone-Related Protein; D010446:Peptide Fragments; D011506:Proteins; C077277:parathyroid hormone-related protein (109-138); D000242:Cyclic AMP