Cloning, expression and immunological characterization of Ory s 1, the major allergen of rice pollen.
We have isolated and characterized a cDNA clone, Ory s 1, encoding a group-1 allergen of rice pollen. The Ory s 1 protein shows significant sequence identity to the major allergen of rye-grass pollen, Lol p 1. RNA gel blot analysis shows that the Ory s 1 gene is expressed in mature anthers, but not in vegetative or other floral tissues tested. Southern blot analysis indicates that this clone represents a member of a small gene family in rice. Western blot analyses of total rice pollen proteins with the group-1 allergen-specific monoclonal 3A2 and IgE antibodies from grass pollen-allergic patients, revealed the presence of cross-reactive antigenic and allergenic epitopes in Ory s 1.
Xu
H
H
School of Botany, University of Melbourne, Parkville, Victoria, Australia.
Theerakulpisut
P
P
Goulding
N
N
Suphioglu
C
C
Singh
M B
MB
Bhalla
P L
PL
D000485:Allergens / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003429:Cross Reactions; D018076:DNA, Complementary; D015870:Gene Expression; D017343:Genes, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family; D012275:Oryza / Q000235:genetics*; D018515:Plant Leaves; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D018517:Plant Roots; D011058:Pollen; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D000485:Allergens; D018076:DNA, Complementary; C096821:Ory s I protein, Oryza sativa; D010940:Plant Proteins