Anisomycin induces phase shifts of circadian pacemaker in primary cultures of rat suprachiasmatic nucleus.
We have developed a cell culture system for the rat suprachiasmatic nucleus, in which a clear circadian oscillation of vasopressin release was observed. Using this culture system, the effect of anisomycin, an inhibitor of protein synthesis, on the circadian rhythm was studied. A phase-delay of more than 15 h could be produced by a 6-h anisomycin pulse. The magnitude of the phase-shift was dependent on the circadian time of the drug treatment and on its dose. The phase-response curve was similar to the response curves that have been measured for protein synthesis inhibitors in other organisms. These results strongly suggest that protein synthesis may be involved in the generation of circadian rhythms in mammals.
Watanabe
K
K
Department of Physiology, Dokkyo University School of Medicine, Tochigi, Japan.
Katagai
T
T
Ishida
N
N
Yamaoka
S
S
D000818:Animals; D000841:Anisomycin / Q000494:pharmacology*; D002478:Cells, Cultured; D002940:Circadian Rhythm; D007700:Kinetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013493:Suprachiasmatic Nucleus / Q000187:drug effects*; D013997:Time Factors
D009419:Nerve Tissue Proteins; D000841:Anisomycin