Purification of a N-acetyl-D-galactosamine specific lectin from the orchid Laelia autumnalis.
From the pseudobulbs of the orchid L. autumnalis a lectin was purified on immobilized porcine mucin with A + H blood group substance. This lectin is a dimeric glycoprotein of M(r) 12,000 with an Sw,20 of 2.2, showing haemagglutinating activity directed mainly to human A1 desialylated erythrocytes. The lectin possesses sugar specificity for N-acetyl-D-galactosamine and also shows high specificity for glycoproteins containing the T (galactose beta 1,3GA1NAc alpha 1,0 Ser/Thr) or the Tn antigen (GalNAc alpha 1,0 Ser/Thr).
Zenteno
R
R
Departamento de Bioquímica, Facultad de Medicina, UNAM, Mexico DF.
Chávez
R
R
Portugal
D
D
Páez
A
A
Lascurain
R
R
Zenteno
E
E
D000017:ABO Blood-Group System; D000116:Acetylgalactosamine; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000737:chemistry; D000957:Antigens, Viral, Tumor / Q000737:chemistry; D002241:Carbohydrates / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D006384:Hemagglutination; D006388:Hemagglutinins / Q000302:isolation & purification*; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification*; D008970:Molecular Weight; D009077:Mucins; D012680:Sensitivity and Specificity; D013329:Structure-Activity Relationship; D013552:Swine
D000017:ABO Blood-Group System; D000596:Amino Acids; D015295:Antigens, Tumor-Associated, Carbohydrate; D000957:Antigens, Viral, Tumor; D002241:Carbohydrates; D006023:Glycoproteins; D006388:Hemagglutinins; D037102:Lectins; D009077:Mucins; C040286:Tn antigen; D000116:Acetylgalactosamine