title: Single-tube nested PCR with room-temperature-stable reagents.
authors.affiliation: Institut für Laboratoriums- und Transfusionsmedizin, Herz- und Diabeteszentrum Nordrhein-Westfalen, Universitätsklinik der Ruhr-Universität Bochum, Bad Oeynhausen, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Wolff
authors.forename: D
authors.initials: D
authors.lastname: Hörnschemeyer
authors.forename: D
authors.initials: D
authors.lastname: Wolff
authors.forename: K
authors.initials: K
authors.lastname: Kleesiek
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000175:diagnosis; D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000097:blood / Q000175:diagnosis; D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral / Q000097:blood* / Q000302:isolation & purification; D004355:Drug Stability; D006679:HIV Seropositivity / Q000097:blood / Q000175:diagnosis; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D016174:Hepacivirus / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000097:blood / Q000175:diagnosis; D006801:Humans; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000097:blood / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase; D012680:Sensitivity and Specificity; D013696:Temperature
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral; D007202:Indicators and Reagents; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase