A micropuncture study of the renal response to haemorrhage in rats: assessment of the role of vasopressin.
The acute effects of haemorrhage (15 ml (kg body wt)-1) on renal function at whole-kidney and single-nephron levels were studied in Inactin-anaesthetized rats. In order to assess the role of vasopressin in mediating the haemodynamic effects, responses in untreated Long-Evans rats were compared with those in Brattleboro rats (which lack circulating vasopressin) and in Long-Evans rats treated with a V1 receptor antagonist. In time-control animals, there were no significant changes in mean arterial pressure (MAP), excretion rates, glomerular filtration rate (GFR), superficial-nephron GFR (SNGFR) or fluid reabsorption in the superficial proximal tubules during the course of the experiment. Following haemorrhage, the immediate reduction in MAP was followed in each group by partial recovery for 30 min; thereafter, MAP was stable. In untreated Long-Evans rats, haemorrhage was followed by a 26% reduction in GFR (P < 0.001, measured 60-150 min post-haemorrhage) and a larger reduction (45%, P < 0.001) in SNGFR, so that the SNGFR/GFR ratio fell significantly ((27.9 +/- 1.9) x 10(-6), control period; (20.2 +/- 2.2) x 10(-6) post-haemorrhage, P < 0.01). Slightly greater reductions in GFR and SNGFR were seen in Brattleboro rats and V1 antagonist-treated Long-Evans rats, which corresponded to slightly greater haemorrhage-induced reductions in blood pressure in these groups; the falls in the SNGFR/GFR ratio were similar to that in untreated Long-Evans rats. In all three groups of bled rats, fractional reabsorption by the proximal convoluted tubule increased slightly 30-60 min after haemorrhage, but during the subsequent period (60-150 min) returned to values indistinguishable from those during the control period. The results suggest that the renal haemodynamic changes that follow moderate haemorrhage include a preferential reduction in the GFR of superficial nephrons. Vasopressin appears to play no role in this response. Increases in fractional reabsorption in the proximal tubules are seen only during the immediate post-haemorrhage period.
Shirley
D G
DG
Department of Physiology, Charing Cross and Westminster Medical School, London, UK.
Walter
S J
SJ
D000042:Absorption; D000818:Animals; D001794:Blood Pressure; D004231:Diuresis; D005919:Glomerular Filtration Rate; D006470:Hemorrhage / Q000503:physiopathology*; D007668:Kidney / Q000503:physiopathology*; D007687:Kidney Tubules, Proximal / Q000378:metabolism; D008297:Male; D009318:Natriuresis; D009399:Nephrons / Q000503:physiopathology; D011188:Potassium / Q000652:urine; D011677:Punctures; D051381:Rats; D011910:Rats, Brattleboro; D011919:Rats, Inbred Strains; D014667:Vasopressins / Q000502:physiology*
D014667:Vasopressins; D011188:Potassium