title: The characteristics of the inhibition of serum cholinesterase by metoclopramide.
abstract: The inhibition of serum cholinesterase by metoclopramide has been previously characterised in vitro at high dilution of the enzyme. We examined the effect of varying enzyme dilution over a range of 1000 fold dilution, and assay temperature at 25 degrees C and 37 degrees C on the fractional inhibition of enzyme activity by metoclopramide. Neither enzyme concentration nor reaction temperature affected this fractional inhibition. Concentrations of metoclopramide producing 50% inhibition of enzyme activity were in the range 1.0-1.9 x 10(-6) M. Lineweaver-Burk analysis of the enzyme reaction suggests that the pattern of this inhibition is competitive.
authors.affiliation: University Department of Anaesthesia, Queens Medical Centre, Nottingham, UK.
authors.forename: S G
authors.initials: SG
authors.lastname: Graham
authors.forename: A W
authors.initials: AW
authors.lastname: Crossley
mesh_terms: D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D002802:Cholinesterases / Q000097:blood / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D006801:Humans; D007700:Kinetics; D008787:Metoclopramide / Q000494:pharmacology*; D013696:Temperature
chemical_list: D002800:Cholinesterase Inhibitors; D002802:Cholinesterases; D008787:Metoclopramide