Recombinant proteins L and LG: efficient tools for purification of murine immunoglobulin G fragments.
In order to improve antibody purification methods, recombinant proteins L and LG were tested in the purification of murine monoclonal immunoglobulin G (IgG) and its fragments. After affinity constant evaluation in different buffer systems, high-performance affinity chromatographic columns were prepared by coupling the proteins to Affi-prep 10 resin and tested with eight different murine monoclonal antibodies and their fragments of different isotypes. Affinity chromatographic experiments confirmed radioimmunoassay results showing that protein L bound 75% of the tested antibody fragments whereas protein LG had affinity for all the tested fragments. These results demonstrate that protein LG is the most powerful Ig-binding tool so far described.
Vola
R
R
Biochemical Oncology Laboratories, SORIN Biomedica, Saluggia (VC), Italy.
Lombardi
A
A
Tarditi
L
L
Björck
L
L
Mariani
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D007128:Immunoglobulin Fragments / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000302:isolation & purification*; D051379:Mice; D010462:Peptostreptococcus; D011994:Recombinant Proteins / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; C055047:Ig L-binding protein, Peptostreptococcus; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D011994:Recombinant Proteins