In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes.
Five of the genes known to encode the enzymes for the synthesis of poly(glycerol phosphate), the major teichoic acid of Bacillus subtilis 168, are organized in two divergently transcribed operons, tagAB and tagDEF. lacZ and gus transcriptional fusions to the first genes of these operons revealed that: (i) in media of different richness, higher growth rates were paralleled by lower transcription levels; (ii) upon transition to stationary phase, the transcription per unit mass of both operons increased abruptly by a factor of about two; and (iii) a rise in temperature was accompanied by decreased transcription of tagA and increased transcription of tagD. Mapping of transcription start points revealed two divergent sigma A-controlled promoters. Although tagD and the neighbouring downstream gene tagE are transcribed from the same promoter, the latter was expressed at a much lower level than the former. Moreover, expression of tagE, and of the translationally coupled tagF, did not increase at the onset of the stationary phase, indicating that additional regulatory signals may act in the intergenic tagD-tagE region. Optimal transcription of these operons appears to require the entire regulatory region, suggesting that tag gene expression may, among other factors, be regulated by the three-dimensional configuration of this segment. The biological implications of these results are discussed.
Mauël
C
C
Institut de Génétique et de Biologie Microbiennes, Lausanne, Switzerland.
Bauduret
A
A
Chervet
C
C
Beggah
S
S
Karamata
D
D
D001412:Bacillus subtilis / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D002473:Cell Wall / Q000737:chemistry* / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005786:Gene Expression Regulation; D005798:Genes, Bacterial; D008074:Lipoproteins / Q000096:biosynthesis / Q000235:genetics; D008954:Models, Biological; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics; D010544:Peroxidases; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000378:metabolism; D013682:Teichoic Acids / Q000032:analysis*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D008074:Lipoproteins; D012808:Sigma Factor; C092406:TagA protein, bacteria; C089973:TagE protein, Vibrio cholerae; D013682:Teichoic Acids; D010544:Peroxidases; C089974:TagD protein, Vibrio cholerae; C063505:RNA polymerase sigma A; D012321:DNA-Directed RNA Polymerases