A comparative study of TK6 human lymphoblastoid and L5178Y mouse lymphoma cell lines in the in vitro micronucleus test.
Micronucleus induction was compared in human lymphoblastoid TK6 and mouse lymphoma L5178Y cell lines treated with model clastogens and spindle poisons, i.e., X-rays, methyl methanesulfonate, ethyl methanesulfonate, mitomycin C, colcemid, and vincristine. The spontaneous micronucleated cell (MNC) frequency was stable and reproducible in both cell lines. All clastogens and spindle poisons studied here induced micronuclei in both cell lines. They increased MNC frequency at lower concentrations or caused a greater increase at the same concentration in TK6 cells. These clastogens and spindle poisons, however, were also more toxic to TK6 than to L5178Y cells and when comparison was based on cytotoxicity, they showed more efficient MNC induction in L5178Y cells. In conclusion, neither cell line was superior to the other, and both of them can be used as target cells in the in vitro micronucleus assay.
Zhang
L S
LS
Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tokyo, Japan.
Honma
M
M
Hayashi
M
M
Suzuki
T
T
Matsuoka
A
A
Sofuni
T
T
D000818:Animals; D001402:B-Lymphocytes; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003703:Demecolcine / Q000633:toxicity; D005020:Ethyl Methanesulfonate / Q000633:toxicity; D006801:Humans; D007940:Leukemia L5178; D008223:Lymphoma; D008741:Methyl Methanesulfonate / Q000633:toxicity; D051379:Mice; D015162:Micronucleus Tests; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity*; D014407:Tumor Cells, Cultured; D014750:Vincristine / Q000633:toxicity; D014965:X-Rays
D009153:Mutagens; D016685:Mitomycin; D014750:Vincristine; D005020:Ethyl Methanesulfonate; D008741:Methyl Methanesulfonate; D003703:Demecolcine