Molecular cloning of a cDNA with a novel domain present in the tre-2 oncogene and the yeast cell cycle regulators BUB2 and cdc16.
In an effort to identify genes that are differentially regulated during mast cell development, subtracted cDNA prepared from wild-type murine P815 mastocytoma cells and a P815 subline that exhibits properties of mast cell differentiation was used to screen mast cell cDNA libraries. Several known mast cell-specific cDNAs were isolated including mast cell carboxypeptidase A (MC-CPA), murine mast cell protease-5 (MMCP-5), and gp49. A novel cDNA, designated Tbc1, was identified that showed differential expression in the two mast cell lines. The amino acid sequence predicted from the cDNA contains a 200 amino acid domain that is homologous to regions in the tre-2 oncogene and the yeast regulators of mitosis, BUB2 and cdc16. The N-terminal region contains a number of cysteine and histidine residues, potentially encoding a zinc finger domain. Tbc1 is a nuclear protein and is expressed in highest levels in hematopoietic cells, testis and kidney. Within these tissues, expression of Tbc1 is cell- and stage-specific. Based on sequence similarity, pattern of expression and subcellular localization, Tbc1 may play a role in the cell cycle and differentiation of various tissues.
Richardson
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Division of Hematology/Oncology, Children's Hospital, Boston, Massachusetts, USA.
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D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D064194:Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome; D001483:Base Sequence; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics; D010450:Endopeptidases; D050981:Erythroid-Specific DNA-Binding Factors; D005656:Fungal Proteins / Q000235:genetics*; D020690:GTPase-Activating Proteins; D012515:Mast-Cell Sarcoma / Q000473:pathology; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology; D015513:Oncogene Proteins; D015514:Oncogene Proteins, Fusion / Q000235:genetics*; D009857:Oncogenes; D011518:Proto-Oncogene Proteins; D011817:Rabbits; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000235:genetics; D014407:Tumor Cells, Cultured; D043222:Ubiquitin Thiolesterase
D064194:Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome; C070351:BUB2 protein, S cerevisiae; C577397:CDC16 protein, S cerevisiae; C577396:Cdc16 protein, mouse; D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D005656:Fungal Proteins; D020690:GTPase-Activating Proteins; D009687:Nuclear Proteins; D015513:Oncogene Proteins; D015514:Oncogene Proteins, Fusion; D011518:Proto-Oncogene Proteins; D029701:Saccharomyces cerevisiae Proteins; C095906:Tbc1d1 protein, mouse; D014157:Transcription Factors; D010450:Endopeptidases; C473330:USP6 protein, human; D043222:Ubiquitin Thiolesterase