Detection of short-chain alpha-hydroxyaldehydic compounds as pentafluorbenzyloxime derivatives in bovine liver.
Pentafluorbenzyloxime derivatization allows fast, gentle and unambiguous identification of alpha-hydroxyaldehydic lipid peroxidation products via GC/MS in biological material. Even 1.5 g of a bovine liver sample is sufficient to detect short-chain 2-hydroxyalkanales resulting from cleavage reactions of dioxygenated fatty acids. Quantification is achieved after secondary derivatization with N-methyl-N-t-butyldimethylsilyltrifluoracetamide (M-t-BSTFA) by mass spectrometry using characteristic ion traces of the derivatives. In addition, the corresponding (n-1)-hydroxy-n-oxo acids, previously unknown in biological material, could be detected.
Loidl-Stahlhofen
A
A
Lehrstuhl Organische Chemie I Universität Bayreuth, NW I, Bayreuth, Germany.
Hannemann
K
K
Spiteller
G
G
D000081:Acetamides; D000447:Aldehydes / Q000032:analysis* / Q000737:chemistry; D000818:Animals; D002417:Cattle; D005463:Fluoroacetates; D008401:Gas Chromatography-Mass Spectrometry; D006898:Hydroxylamines; D015227:Lipid Peroxidation; D008099:Liver / Q000737:chemistry*; D015394:Molecular Structure; D017646:Organosilicon Compounds; D005466:Fluorocarbons
D000081:Acetamides; D000447:Aldehydes; D005463:Fluoroacetates; D006898:Hydroxylamines; D017646:Organosilicon Compounds; C010277:Florox Reagent; D005466:Fluorocarbons; C059151:N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide