Follicular development and ovulation in sows: effect of hCG and GnRH treatment.
Follicular growth, chronology of ovulation and embryo morphology were compared in sows ovulating spontaneously and sows, in which the ovulation was attempted induced by hCG or GnRH. Indwelling catheters were placed on day 1 (weaning = day 0) in the ear veins of 18 sows, which were then randomly divided into 3 groups: a control group (N = 6), a group (N = 6) given 750 iu hCG (Physex) im 76h after weaning (hCG group) and a group (N = 6) given 500 micrograms GnRH (Fertagyl) im 76h (N = 3) or 100h after weaning (N = 3) (GnRH group). Follicular diameter and time of ovulation were monitored by ultrasonography every 4h from day 3 until ovulation or development of cysts by means of a sector scanner fitted with a 5.0/7.5 MHz multiangle probe. Heat detection was performed every 8h from day 3 until ovulation. On day 13, the sows were slaughtered, the number of corpora luteae (CL) was counted, and embryos were flushed from the uteri. The control group showed clear heat symptoms, and on day 3, the follicles were typically 3-7 mm and grew up to 7-10 mm over 2 days, where they remained for approximately 24h until ovulation took place 41h +/- 9h after first sign of standing heat. The hCG group exhibited no signs of heat, and the follicles only reached 5-8 mm in diameter at time of ovulation, which occurred 40h +/- 1h after hCG-injection. The GnRH group exhibited inconsistent signs of heat, and the follicles reached a maximum size of 7-12 mm in diameter where they remained for more than 24h. Only 2 sows in this group ovulated within 84-92h after the GnRH injection, and development of bursa cysts and cystic follicles was a common finding. The average number of CL was 18.2 +/- 5.7 per sow (N = 16, range: 3-27) with no significant difference between the groups. Total embryo recovery was 79 +/- 13% with no significant difference between groups. The embryo diversity calculated as standard deviation of the maximum diameter was higher in the hCG group as compared with the control group.(ABSTRACT TRUNCATED AT 400 WORDS)
Nissen
A K
AK
Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Lehn-Jensen
H
H
Hyttel
P
P
Greve
T
T
D000818:Animals; D001757:Blastomeres / Q000502:physiology; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D004622:Embryo, Mammalian; D004971:Estrus / Q000502:physiology; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D006080:Ovarian Follicle / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000254:growth & development; D010060:Ovulation / Q000502:physiology*; D010062:Ovulation Induction / Q000662:veterinary*; D013552:Swine / Q000502:physiology*; D013997:Time Factors; D014463:Ultrasonography
D006063:Chorionic Gonadotropin; D007987:Gonadotropin-Releasing Hormone