The use of isoelectric focusing on polyacrylamide gel for the enzymatic analysis of 'Old World' Leishmania species.
Using isoelectric focusing (IEF), a high resolution electrophoresis technique, we analysed 6 enzymes of 24 cloned strains representing all major taxa of 'Old World' Leishmania. The comparison of enzymatic patterns obtained with IEF and starch gel electrophoresis showed that IEF is a more discriminatory and more informative technique for the enzymatic analysis of Leishmania strains; it can detect very slight differences between 2 electromorphs not revealed with starch gel electrophoresis. Moreover, IEF detected several multi-banded patterns which appeared as single bands with starch gel electrophoresis. These multi-banded patterns could not be the result of strain heterogeneity since all the strains had been cloned. Their significance in the biology of Leishmania is discussed.
Piarroux
R
R
Laboratoire d'Ecologie Médicale et Pathologie Parasitaire, Faculté de Médecine, Université de Montpellier, France.
Trouvé
V
V
Pratlong
F
F
Martini
A
A
Lambert
M
M
Rioux
J A
JA
D000818:Animals; D004592:Electrophoresis, Starch Gel; D007525:Isoelectric Focusing / Q000379:methods; D007527:Isoenzymes / Q000032:analysis; D007891:Leishmania / Q000145:classification* / Q000201:enzymology
D007527:Isoenzymes