Crude urinary human chorionic gonadotropin contains variant forms of HCG with low sialic acid content that exhibit an increased thyrotropic activity in CHO cells expressing the human TSH receptor.
Hyperthyroidism occurs in association with pregnancy or trophoblastic tumours. This is due to the secretion of thyroid stimulators by trophoblastic cells, most likely hCG or a variant form of hCG. In the present studies we sought to identify hCG variants with enhanced thyrotropic activity contained in crude hCG extract from pregnancy urine (hCGc). Such studies seem now feasible, because highly sensitive assays employing CHO cells transfected with the recombinant human TSH receptor recently became available. Initially, we found the activity of hCGc to both inhibit the binding of 125I-bTSH to CHO-TSHr cells and to stimulate the cAMP release by the cells to be increased, compared to highly purified hCG (hCGp), which was tested in comparable immunological concentrations. We then processed hCGc on a DEAE-52 anionexchange column to separate materials of interest, termed hCGv, from hCGp. HCGv was further purified by gel chromatography, and found to be enriched in terms of both, its holo-hCG immunoactivity and its TSH binding inhibiting activity, compared to hCGc where it was derived from. It also proved more potent than hCGp to bind to recombinant hTSH receptor and to stimulate adenylate cyclase activity in CHO-TSHr cells. Enzymatic desialylation was able to increase the potency of both hCGv and hCGp, and rendered the two desialylated hCG forms nearly equipotent. Isoelectric focusing and direct measurement of sialic acid contents revealed hCGv to be less sialylated than hCGp.(ABSTRACT TRUNCATED AT 250 WORDS)
Poertl
S
S
Medical Department II, Klinikum Grosshadern, University of Munich, Germany.
Liss
I
I
Mann
K
K
Hoermann
R
R
D000818:Animals; D016466:CHO Cells; D006063:Chorionic Gonadotropin / Q000737:chemistry* / Q000502:physiology* / Q000652:urine; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D005260:Female; D006801:Humans; D019158:N-Acetylneuraminic Acid; D011247:Pregnancy; D011989:Receptors, Thyrotropin / Q000378:metabolism*; D012794:Sialic Acids / Q000032:analysis*; D013972:Thyrotropin / Q000037:antagonists & inhibitors / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D011989:Receptors, Thyrotropin; D012794:Sialic Acids; D013972:Thyrotropin; D000242:Cyclic AMP; D019158:N-Acetylneuraminic Acid