Sequence and expression of the bpdC1C2BADE genes involved in the initial steps of biphenyl/chlorobiphenyl degradation by Rhodococcus sp. M5.
The nucleotide (nt) sequence of the bpdC1C2BADE genes which encode the first three enzymes in the biphenyl (BP) degradation pathway of Gram+ Rhodococcus sp. M5 (formerly Arthrobacter M5) was determined. Except for the ferredoxin component (BpdB) of the initial BP dioxygenase, the predicted amino acid (aa) sequences of the remaining proteins are found to be more closely related to the counterpart proteins (TodC1C2BADE) present in the toluene-degrader, Pseudomonas putida F1, than those of three BP-degrading pseudomonads. The cloned bpd genes were verified by their expression in the Escherichia coli T7 RNA polymerase/promoter system. In E. coli, BpdA was able to complement TodC1C2B in indigo biosynthesis, although the M5 native or cloned BP dioxygenase does not carry out this reaction.
Wang
Y
Y
Biotechnology Research Institute, National Research Council of Canada, Montréal, Québec, Canada.
Garnon
J
J
Labbé
D
D
Bergeron
H
H
Lau
P C
PC
D001426:Bacterial Proteins / Q000235:genetics*; D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000378:metabolism*; D003001:Cloning, Molecular; D004275:DNA, Ribosomal / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D007506:Iron-Sulfur Proteins; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism; D012240:Rhodococcus / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
D001426:Bacterial Proteins; D001713:Biphenyl Compounds; D004275:DNA, Ribosomal; D007506:Iron-Sulfur Proteins; D010105:Oxygenases; D011078:Polychlorinated Biphenyls; D012336:RNA, Ribosomal, 16S; D011994:Recombinant Proteins; C010574:biphenyl; C074161:biphenyl-2,3-dioxygenase