title: High incidence of human papillomavirus in 146 cervical carcinomas. A study using three different pairs of consensus primers, and detecting viral genomes with putative deletions.
abstract: Polymerase chain reaction (PCR) primer sets and probe-cocktails were used for human papillomavirus (HPV) detection and typing of 146 fresh frozen biopsies of cervical carcinoma. We obtained a high detection rate (96%) by using three sets of consensus primer pairs directed at the L1 and E1 regions of HPV and by probing with a cocktail of random-labelled consensus and type-specific PCR products derived from HPV plasmids. In addition, we performed type-specific PCR amplification with E6-E7 primers. The procedure was designed to detect all HPV-positive cases in a rapid, sensitive and specific way. In addition, by using different regions for amplification, we detected cases with putative genomic deletions in HPV. All the negative PCR and DNA isolation controls were negative. The six negative samples were negative with all probe-cocktails and type-specific primers and three of these negative samples were clear cell carcinomas. The detection rate was similar in squamous carcinomas and in adenocarcinomas and type 16 was most common (65%) in both types of carcinoma. There were no double infections of human papillomavirus 16 and 18.
authors.affiliation: Department of Pathology, Norwegian Radium Hospital, Oslo, Norway.
authors.forename: F
authors.initials: F
authors.lastname: Karlsen
authors.forename: G
authors.initials: G
authors.lastname: Kristensen
authors.forename: R
authors.initials: R
authors.lastname: Holm
authors.forename: M
authors.initials: M
authors.lastname: Chitemerere
authors.forename: A
authors.initials: A
authors.lastname: Berner
authors.forename: B M
authors.initials: BM
authors.lastname: Hagmar
mesh_terms: D000230:Adenocarcinoma / Q000821:virology*; D018262:Adenocarcinoma, Clear Cell / Q000821:virology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000821:virology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D017384:Sequence Deletion; D002583:Uterine Cervical Neoplasms / Q000821:virology*