DNA strand breakage and base modification induced by hydrogen peroxide treatment of human respiratory tract epithelial cells.
Treatment of human respiratory tract epithelial cells with H2O2 led to concentration-dependent DNA strand breakage that was highly-correlated with multiple chemical modifications of all four DNA bases, suggesting that damage is due to hydroxyl radical, OH. However, the major base damage occurred to adenine. Hence, conclusions made about the occurrence and the extent of oxidative DNA damage on the basis only of changes in 8-hydroxyguanine should be approached with caution.
Spencer
J P
JP
Neurodegenerative Disease Research Centre, University of London King's College, UK.
Jenner
A
A
Chimel
K
K
Aruoma
O I
OI
Cross
C E
CE
Wu
R
R
Halliwell
B
B
D001980:Bronchi / Q000166:cytology; D002461:Cell Line, Transformed; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004848:Epithelium; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D017665:Hydroxyl Radical / Q000633:toxicity; D010084:Oxidation-Reduction
D017665:Hydroxyl Radical; D004247:DNA; D006861:Hydrogen Peroxide