title: Membrane fluidization by animycotic bifonazole.
abstract: Calorimetry, nuclear magnetic resonance and X-ray diffraction techniques have been used to obtain thermodynamic and structural information on dipalmitoyl phosphatidylcholine (DPPC) liposomes doped by the antimycotic drug bifonazole in the range 0 < R < 1, where R = moles of bifonazole/moles of DPPC. The technique of spin labeling electron spin resonance (ESR) has also been used to study permeability and fluidity properties. The decrease of the cooperativity at the gel to liquid crystalline phase transition, as shown by ESR an DSC measurements, indicates that bifonazole imparts higher fluidity to the lipid matrix. Increase in permeability of ascorbate ions, after incorporation of bifonazole in the membrane, has been detected by ESR experiments using spin label 5-SASL. 13C NMR spectra indicate that the drug molecule is highly immobilized. X-ray diffraction and freeze fracture TEM results show that the equilibrated phase at room temperature is lamellar and unidimensional together with the presence of small particles and pits of uniform size. A marked hysteresis is evident in the formation of this phase.
authors.affiliation: Dipartimento di Scienze dei Materiali e della Terra, University, Ancona Italy.
authors.forename: G
authors.initials: G
authors.lastname: Albertini
authors.forename: G
authors.initials: G
authors.lastname: Bossi
authors.forename: B
authors.initials: B
authors.lastname: Dubini
authors.forename: R S
authors.initials: RS
authors.lastname: Phadke
authors.forename: M G
authors.initials: MG
authors.lastname: Ponzi Bossi
authors.forename: A
authors.initials: A
authors.lastname: Pugnaloni
authors.forename: S
authors.initials: S
authors.lastname: Srivastava
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry*; D000935:Antifungal Agents / Q000494:pharmacology; D002152:Calorimetry, Differential Scanning; D004578:Electron Spin Resonance Spectroscopy; D007093:Imidazoles / Q000494:pharmacology; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008560:Membrane Fluidity / Q000187:drug effects*; D008854:Microscopy, Electron; D008954:Models, Biological; D010539:Permeability; D013816:Thermodynamics; D014961:X-Ray Diffraction
chemical_list: D000935:Antifungal Agents; D007093:Imidazoles; D008081:Liposomes; D015060:1,2-Dipalmitoylphosphatidylcholine; C036596:bifonazole