Simultaneous determination of theophylline, enoxacin and ciprofloxacin in human plasma and saliva by high-performance liquid chromatography.
A simple reversed-phase high-performance liquid chromatographic method has been developed for the simultaneous determination of theophylline, ciprofloxacin and enoxacin in plasma and saliva. The biological fluid samples were extracted with methylene chloride-isopropyl alcohol prior to isocratic chromatography on a Waters C18 mu Bondapak column. Ultraviolet detection was carried out at 268 nm. The assay is linear for ciprofloxacin and enoxacin (0.05-10 micrograms/ml), and theophylline (0.1-20 micrograms/ml). The assay can be used to investigate the interaction of these two fluoroquinolones with theophylline.
Zhai
S
S
Clinical Pharmacology and Gerontology Research Unit, Department of Veterans Affairs Medical Center, Boise, ID 83702, USA.
Korrapati
M R
MR
Wei
X
X
Muppalla
S
S
Vestal
R E
RE
D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood; D001993:Bronchodilator Agents / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000032:analysis* / Q000097:blood; D015365:Enoxacin / Q000032:analysis* / Q000097:blood; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012463:Saliva / Q000378:metabolism*; D013806:Theophylline / Q000032:analysis* / Q000097:blood
D000890:Anti-Infective Agents; D001993:Bronchodilator Agents; D015365:Enoxacin; D002939:Ciprofloxacin; D013806:Theophylline