title: Analysis of pyrrolizidine alkaloids: a competitive enzyme-linked immunoassay (ELISA) for the quantitative determination of some toxic pyrrolizidine alkaloids.
abstract: A highly sensitive and specific competitive enzyme-linked immunoassay (ELISA) for the determination of retrorsine, senecionine, and integerrimine in the nanogram range has been developed. Pyrrolizidine alkaloids were detected in Senecio rupestris. No cross-reactivity was seen with either retrosine N-oxide, otonecine alkaloids, or other macrocyclic pyrrolizidine alkaloids.
authors.affiliation: Pharmazeutisches Institut, Universität Bonn, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Roeder
authors.forename: T
authors.initials: T
authors.lastname: Pflueger
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D011763:Pyrrolizidine Alkaloids / Q000032:analysis*; D011817:Rabbits; D012015:Reference Standards
chemical_list: D011763:Pyrrolizidine Alkaloids; C003300:retrorsine