title: pH and the cAMP-dependent protein kinase mediate growth phase induction of the cytochrome c oxidase subunit VI gene, COX6, in Saccharomyces cerevisiae.
abstract: Respiratory adaptation in Saccharomyces cerevisiae is a complex genetic program that ensures ATP synthesis in a glucose-depleted environment. ATP is generated during respiration by the mitochondrial electron transport chain which is induced by respiratory adaptation. We have studied the terminal enzyme in mitochondrial electron transport, cytochrome c oxidase, from S. cerevisiae. Because subunits in this multisubunit enzyme are coordinately regulated, we have focused upon the well characterized subunit VI gene, COX6. In yeast, COX6 transcription is regulated by several factors thought to mediate respiratory adaptation including growth phase induction, oxygen dependence, and glucose repression. In the present study, we found that in addition to these known regulators, COX6 expression also depends upon pH and the cAMP-dependent protein kinase which may comprise additional factors signaling respiratory adaptation.
authors.affiliation: Webb-Waring Institute for Biomedical Research, Denver, Colorado 80262, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Wright
authors.forename: S L
authors.initials: SL
authors.lastname: Simpson
authors.forename: B D
authors.initials: BD
authors.lastname: Lanoil
mesh_terms: D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000096:biosynthesis* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005838:Genotype; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D046911:Macromolecular Substances; D015345:Oligonucleotide Probes; D011993:Recombinant Fusion Proteins; D003576:Electron Transport Complex IV; D017868:Cyclic AMP-Dependent Protein Kinases; D001616:beta-Galactosidase