title: Characterization of the humoral immune response to porcine reproductive and respiratory syndrome (PRRS) virus infection.
abstract: The development of the humoral immune response against porcine reproductive and respiratory syndrome (PRRS) virus was monitored by an indirect fluorescent antibody (IFA) test, immunoperoxidase monolayer assay (IPMA), enzyme-linked immunosorbent assay (ELISA), and serum virus neutralization (SVN) test over a 105-day period in 8 pigs experimentally infected with ATCC strain VR-2402. Specific antibodies against PRRS virus were first detected by the IFA test, IPMA, ELISA, and the SVN test 9-11, 5-9, 9-13, and 9-28 days postinoculation (PI), respectively, and reached their maximum values by 4-5, 5-6, 4-6, and 10-11 weeks PI, respectively, thereafter. After reaching maximum value, all assays showed a decline in antibody levels. Assuming a constant rate of antibody decay, it was estimated by regression analysis that the ELISA, IFA, IPMA, and SVN antibody titers would approach the lower limits of detection by approximately days 137, 158, 324, and 356 PI, respectively. In this study, the immunoperoxidase monolayer assay appeared to offer slightly better performance relative to the IFA test, ELISA, and SVN test in terms of earlier detection and slower rate of decline in antibody titers. Western immunoblot analysis revealed that antibody specific for the 15-kD viral protein was present in all pigs by 7 days PI and persisted throughout the 105-day observation period. Initial detection of antibodies to the 19-, 23-, and 26-kD proteins varied among pigs, ranging from 9 to 35 days PI. Thereafter, the antibody responses to these 3 viral proteins of PRRS virus continued to be detected throughout the 105-day study period.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Microbiology, Immunology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
authors.forename: K J
authors.initials: KJ
authors.lastname: Yoon
authors.forename: J J
authors.initials: JJ
authors.lastname: Zimmerman
authors.forename: S L
authors.initials: SL
authors.lastname: Swenson
authors.forename: M J
authors.initials: MJ
authors.lastname: McGinley
authors.forename: K A
authors.initials: KA
authors.lastname: Eernisse
authors.forename: A
authors.initials: A
authors.lastname: Brevik
authors.forename: L L
authors.initials: LL
authors.lastname: Rhinehart
authors.forename: M L
authors.initials: ML
authors.lastname: Frey
authors.forename: H T
authors.initials: HT
authors.lastname: Hill
authors.forename: K B
authors.initials: KB
authors.lastname: Platt
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000917:Antibody Formation; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology; D015153:Blotting, Western / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005831:Genital Diseases, Female / Q000276:immunology / Q000662:veterinary / Q000821:virology; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests; D012141:Respiratory Tract Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases; D013577:Syndrome; D014035:Togaviridae / Q000276:immunology*; D014036:Togaviridae Infections / Q000276:immunology / Q000662:veterinary*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral