title: Genome organisation in the murine sperm nucleus.
abstract: The organisation of DNA sequences in the murine sperm nucleus was studied using in situ hybridisation of biotinylated DNA probes. The efficiency of this reaction was assessed using a dispersed repetitive DNA probe. Telomeric DNA was distributed around the nucleus. Centromeric and ribosomal DNA sequences occupied restricted domains in the sperm nucleus. DNA sequences for a transgene and a cluster of homeogenes occupied different, and rather less defined, domains. Together these results imply that both repetitive and protein-coding sequences are arranged in the nucleus in an ordered fashion.
authors.affiliation: Department of Development and Signalling, Babraham Institute, Cambridge, UK.
authors.forename: C
authors.initials: C
authors.lastname: Jennings
authors.forename: D
authors.initials: D
authors.lastname: Powell
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000737:chemistry* / Q000648:ultrastructure; D002503:Centromere; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D004275:DNA, Ribosomal / Q000032:analysis; D005796:Genes; D016678:Genome; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D051379:Mice / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D013094:Spermatozoa / Q000737:chemistry / Q000648:ultrastructure*; D016615:Telomere
chemical_list: D015342:DNA Probes; D004275:DNA, Ribosomal; D004247:DNA