title: A second community outbreak of waterborne giardiasis in Canada and serological investigation of patients.
abstract: A waterborne outbreak of giardiasis which occurred 5 years after another in the same town in Canada was investigated. Sera from residents defined as cases or non-cases were tested by enzyme-linked immunosorbent assay (ELISA) and compared with sera from symptomatic and asymptomatic control groups. The outbreak-associated Giardia isolate was retrieved from contaminated drinking water and antigen from this strain was used in the serological investigation. Up to 84% of cases were identified by ELISA. More cases were identified by elevated immunoglobulin (Ig) G than by either elevated anti-Giardia IgA or IgM levels. Residents of the community infected during the first outbreak were significantly less likely to have been reinfected during the second outbreak. This is the first report of a second waterborne outbreak occurring in a community and results of the investigations are consistent with an acquired, protective immunity lasting at least 5 years.
authors.affiliation: Department of Pathology, University of British Columbia, Vancouver, Canada.
authors.forename: J L
authors.initials: JL
authors.lastname: Isaac-Renton
authors.forename: L F
authors.initials: LF
authors.lastname: Lewis
authors.forename: C S
authors.initials: CS
authors.lastname: Ong
authors.forename: M F
authors.initials: MF
authors.lastname: Nulsen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis; D001955:British Columbia / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005872:Giardia / Q000302:isolation & purification; D005873:Giardiasis / Q000453:epidemiology* / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007223:Infant; D008297:Male; D008875:Middle Aged; D014881:Water Supply
chemical_list: D000913:Antibodies, Protozoan; D007136:Immunoglobulins