title: A novel strategy to identify maternal and paternal inheritance in the mouse.
abstract: A novel strategy for identifying proteins which reveal maternal or paternal inheritance in the mouse is presented. Using two-dimensional electrophoresis we investigated protein expression patterns of adult liver and different embryonic and extraembryonic tissue in C57BL/6Crl and in DBA/2Crl mice, as well as in their reciprocal hybrids. We found three groups of protein spots which showed maternal or paternal inheritance of quantitative variations. These proteins were characterized by N-terminal or internal amino acid sequencing, by determination of the amino acid composition, by glycoprotein staining and RNA expression analysis. The three proteins identified were: alpha-enolase, cyclophilin and beta-group hemoglobins. The parental effects observed for alpha-enolase and cyclophilin were found to be due to parent-specific post-translational modifications of these proteins. For the beta-group hemoglobins our results suggested parental effects on the transcriptional level.
authors.affiliation: Institut für Toxikologie und Embryopharmakologie, Freie Universität Berlin, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Römer
authors.forename: P
authors.initials: P
authors.lastname: Jungblut
authors.forename: W
authors.initials: W
authors.lastname: Reik
authors.forename: A
authors.initials: A
authors.lastname: Otto
authors.forename: J
authors.initials: J
authors.lastname: Klose
mesh_terms: D000591:Amino Acid Isomerases / Q000032:analysis* / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000032:analysis* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D005333:Fetus; D006454:Hemoglobins / Q000032:analysis* / Q000235:genetics; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D019696:Peptidylprolyl Isomerase; D010751:Phosphopyruvate Hydratase / Q000032:analysis* / Q000235:genetics; D011499:Protein Processing, Post-Translational
chemical_list: D002352:Carrier Proteins; D006454:Hemoglobins; D010751:Phosphopyruvate Hydratase; D000591:Amino Acid Isomerases; D019696:Peptidylprolyl Isomerase