Rolling in P-selectin-deficient mice is reduced but not eliminated in the dorsal skin.
P-selectin-mediated rolling is believed to be important in the recruitment of leukocytes to tissue after ischemia-reperfusion injury. The dorsal skin chamber was used to examine differences in the rolling and stable adhesion of circulating leukocytes in subcutaneous (SC) vessels of P-selectin-deficient and age-matched wild-type mice, both under basal conditions and after ischemia-reperfusion. Rolling in the postcapillary venules in SC tissue of P-selectin-deficient mice was significantly lower than that in wild-type mice under the basal conditions and post-ischemia-reperfusion (P < .05), but was not eliminated by the deletion of the P-selectin gene. No significant difference between P-selectin-deficient and wild-type mice in shear rate or leukocyte-endothelial adhesion was observed up to 24 hours after ischemia-reperfusion. These results show that P-selectin-mediated rolling is not a prerequisite for ischemia-reperfusion-induced leukocyte-endothelial adhesion in the skin.
Yamada
S
S
Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, Boston 02114, USA.
Mayadas
T N
TN
Yuan
F
F
Wagner
D D
DD
Hynes
R O
RO
Melder
R J
RJ
Jain
R K
RK
D000818:Animals; D001415:Back; D002448:Cell Adhesion; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000166:cytology*; D007511:Ischemia / Q000503:physiopathology*; D007962:Leukocytes / Q000737:chemistry / Q000166:cytology*; D051379:Mice; D019007:P-Selectin / Q000502:physiology*; D015427:Reperfusion Injury / Q000503:physiopathology*; D012867:Skin / Q000098:blood supply*; D012884:Skin Window Technique; D014699:Venules
D019007:P-Selectin