Serological abnormalities induced by engraftment of viable motheaten hematopoietic cells in nude beige recipients.
C57BL/6J (B6) mice homozygous for the viable motheaten (mev) mutation are short-lived and display severe immunodeficiency, autoimmunity and inflammatory disease. B6 mice doubly homozygous for the nude (nu) and beige (bg) mutations (nubg mice) are also short-lived and immunodeficient. Nevertheless, grafts of mev lympho-hematopoietic cells increased life expectancy of nubg recipients. Such [mev --> nubg] chimeras did not develop any mev-like inflammatory pathology but showed autoimmunity features, particularly hyperglobulinemia which, unlike the mev one, was due to IgG rather than IgM. Serological studies of [mev IgHb --> nubg Igha] chimeras surprisingly revealed the exclusive host B-cell origin of the IgG2a overproduced by these chimeras. Yet, about half of such chimera serum IgM being IgMb, mev B cells had actually engrafted the nubg hosts. Together with the lack of transfer of the inflammatory pathology, this suggests that a non-mev environment might succeed acting as a regulator of some mev-induced dysfunctions.
Velin
D
D
Laboratoire d'immunologie, Université de Strasbourg, France.
Kuntz
L
L
Loor
F
F
D000818:Animals; D000974:Antibodies, Antinuclear / Q000097:blood; D000918:Antibody Specificity / Q000235:genetics; D001402:B-Lymphocytes / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000276:immunology; D005260:Female; D005808:Genes, Recessive / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D007074:Immunoglobulin G / Q000096:biosynthesis; D007132:Immunoglobulin Isotypes / Q000097:blood* / Q000235:genetics; D007075:Immunoglobulin M / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008819:Mice, Nude; D011828:Radiation Chimera
D000974:Antibodies, Antinuclear; D004277:DNA, Single-Stranded; D007074:Immunoglobulin G; D007132:Immunoglobulin Isotypes; D007075:Immunoglobulin M