title: Enzymatic properties of lipase and characteristics production by Lactobacillus delbrueckii subsp. bulgaricus.
abstract: Some properties of an extracellular lipase produced by Lactobacillus delbrueckii subsp. bulgaricus were studied. Maximum enzyme activity was found against olive and butter oil as enzyme substrates. Addition of 9% acacia gum, 0.1% Na-deoxycholate and 0.01 M CaCl2 to the enzyme reaction mixture increased-lipase activity from 5.3 to 14.5 (FFA/mg protein/minute) at pH 6.0 and at 40 degrees C. Maximum lipase production was reached in the presence of glucose as a sole source of carbon, wheat bran as nitrogen source, olive oil as a sole lipid source and butyric acid as fatty acid supporting the growth medium. An initial pH value of the culture medium of 6.0 and a temperature of 35 degrees C gave the highest lipolytic activity.
authors.affiliation: Microbiology Department, Faculty of Agriculture, Mansoura University, Egypt.
authors.forename: M M
authors.initials: MM
authors.lastname: el-Sawah
authors.forename: A A
authors.initials: AA
authors.lastname: Sherief
authors.forename: S M
authors.initials: SM
authors.lastname: Bayoumy
mesh_terms: D002087:Butyrates; D020148:Butyric Acid; D002122:Calcium Chloride / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000494:pharmacology; D004043:Dietary Fiber; D005947:Glucose; D006170:Gum Arabic / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000187:drug effects / Q000201:enzymology*; D008049:Lipase / Q000096:biosynthesis / Q000378:metabolism*; D000069463:Olive Oil; D010938:Plant Oils; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D002087:Butyrates; D004043:Dietary Fiber; D000069463:Olive Oil; D010938:Plant Oils; D003840:Deoxycholic Acid; D020148:Butyric Acid; D006170:Gum Arabic; D008049:Lipase; D005947:Glucose; D002122:Calcium Chloride