title: Evidence that the antigen receptors of cytotoxic T lymphocytes interact with a common recognition pattern on the H-2Kb molecule.
abstract: Recognition of class I MHC antigens involves interaction between TCRs of cytotoxic T lymphocytes (CTL) and the two alpha helices of MHC molecules. Using a combined panel of H-2Kb mutants selected by either a CTL clone or MAbs, we have shown evidence that the TCRs of 59 Kb-specific CTL clones shared a common binding pattern on the H-2Kb molecule. Mutations of amino acid residues at the C-terminal regions, but not the N-terminal regions, of the alpha helices abrogated the recognition by the majority of the clones. The data suggests that TCRs predominantly recognize the class I MHC molecule with an orientation that is parallel to the beta-pleated strands and diagonal to the alpha helices.
authors.affiliation: Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA.
authors.forename: R
authors.initials: R
authors.lastname: Sun
authors.forename: S E
authors.initials: SE
authors.lastname: Shepherd
authors.forename: S S
authors.initials: SS
authors.lastname: Geier
authors.forename: C T
authors.initials: CT
authors.lastname: Thomson
authors.forename: J M
authors.initials: JM
authors.lastname: Sheil
authors.forename: S G
authors.initials: SG
authors.lastname: Nathenson
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D003601:Cytotoxicity Tests, Immunologic; D006183:H-2 Antigens / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000276:immunology* / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism*
chemical_list: D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D011948:Receptors, Antigen, T-Cell