title: Dynamics of the biosynthesis of methylursubin in plant cells employing in vivo 13CNMR without labelling.
abstract: In vivo NMR experiments with a digital 600 MHz instrument, exploiting the natural abundance of 13C, allowed us for the first time to follow the biosynthesis of the newly detected glycoside, methylursubin (4-methoxyphenyl-O-beta-D-primeveroside), from 4-methoxyphenol through the intermediate methylarbutin in cell suspensions of the Indian medical plant, Rauwolfia serpentina. The metabolic dynamics indicate that, within 48 hr, 4-methoxyphenol is almost completely converted into the primeveroside, methylursubin. Because of the higher sensitivity at 150.9 MHz compared to that at 100.6 MHz, measuring times could be reduced to 1.5 hr. This allows detailed monitoring of the conversion of 4-methoxyphenol with an excellent signal-to-noise ratio.
authors.affiliation: Lehrstuhl für Pharmazeutische Biologie, Johannes Gutenberg-Universität Mainz, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Lutterbach
authors.forename: J
authors.initials: J
authors.lastname: Stöckigt
mesh_terms: D000840:Anisoles / Q000737:chemistry / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D002478:Cells, Cultured; D004187:Disaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010946:Plants, Medicinal; D011926:Rauwolfia / Q000378:metabolism*; D013055:Spectrophotometry, Infrared
chemical_list: D000840:Anisoles; D002247:Carbon Isotopes; D004187:Disaccharides; C096643:methylursubin