Site-specific transgene insertion: an approach.
Methods to improve the production of transgenic animals are being developed. Conventional transgenesis, involving microinjection of DNA into fertilized eggs, has a number of limitations. These result from the inability to control both the site of transgene insertion and the number of gene copies inserted. The approach described seeks to overcome these problems and to allow single copy insertion of transgenes into a defined site in animal genomes. The method involves the use of embryonic stem cells, gene targeting and the FLP recombinase system.
Wigley
P
P
Bresatec laboratories, Department of Biochemistry, University of Adelaide, Australia.
Becker
C
C
Beltrame
J
J
Blake
T
T
Crocker
L
L
Harrison
S
S
Lyons
I
I
McKenzie
Z
Z
Tearle
R
R
Crawford
R
R
D000818:Animals; D030801:Animals, Genetically Modified; D004254:DNA Nucleotidyltransferases; D004622:Embryo, Mammalian; D018390:Gene Targeting; D018014:Gene Transfer Techniques; D051379:Mice; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013234:Stem Cells; D015053:Zygote
D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase