title: Effect of hypoosmolality on the abundance, poly(A) tail length and axonal targeting of arginine vasopressin and oxytocin mRNAs in rat hypothalamic magnocellular neurons.
abstract: Arginine vasopressin (AVP) and oxytocin (OT) mRNAs are targeted to the axonal compartment of rat hypothalamic magnocellular neurons. Salt-loading results in a considerable rise in hypothalamic and axonal AVP mRNA but only a moderate increase for axonal OT mRNA. Here we report that hypoosmolality gives rise to a rapid decrease of axonal AVP encoding transcripts to undetectable levels after 2 weeks. The levels of OT mRNA in the axonal compartment did not change significantly. In the hypothalamus the mRNA for AVP also decreased. The size of the poly(A) tract of AVP encoding transcripts appeared to be strictly correlated with plasma osmolality. In contrast, the amount and size of OT encoding mRNAs were only moderately or not influenced by hypoosmolar stimuli.
authors.affiliation: Danish Biotechnology Centre for Signal Peptide Research, Department of Medical Physiology, University of Copenhagen, Denmark.
authors.forename: P C
authors.initials: PC
authors.lastname: Svane
authors.forename: N A
authors.initials: NA
authors.lastname: Thorn
authors.forename: D
authors.initials: D
authors.lastname: Richter
authors.forename: E
authors.initials: E
authors.lastname: Mohr
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000096:biosynthesis*; D001369:Axons / Q000187:drug effects / Q000378:metabolism*; D015152:Blotting, Northern; D003894:Deamino Arginine Vasopressin / Q000008:administration & dosage / Q000494:pharmacology; D007031:Hypothalamus / Q000378:metabolism*; D007038:Hypotonic Solutions; D007263:Infusions, Parenteral; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010121:Oxytocin / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
chemical_list: D007038:Hypotonic Solutions; D012333:RNA, Messenger; D001127:Arginine Vasopressin; D010121:Oxytocin; D003894:Deamino Arginine Vasopressin