title: Characteristics of tetanic contractions in caffeine-treated rat myocardium.
abstract: Skinned fiber preparations are used to obtain the maximal contractile activation of isolated myocardial preparations. Tetanic contractions elicited in the presence of sarcoplasmic reticulum inhibitors have also been used as an alternative method to produce maximal active tension in the intact myocardium. In this work our purpose was to define the best conditions to obtain tetanic contractions in the rat myocardium and to compare the influence of muscle length and inotropic interventions (Ca2+ and Bay K 8644) in the tension produced in twitches and tetanic contractures. Papillary muscles were mounted in a perfusion chamber to record isometric force. Tetanic contractions were elicited by using suprathreshold stimulation with rectangular pulses (10 ms duration) at 5 Hz in the presence of 2.5 mM caffeine. Caffeine depressed the twitch tension but the tetanic tension was similar to that produced under steady-state stimulation (0.5 Hz) in control conditions. Tetanic and twitch tensions were similar along the whole extension of the length-tension curve and under the positive inotropic effects produced by Ca2+ (0.25 to 3.75 mM) or by the Ca(2+)-channel agonist Bay K 8644 (1 microM). During long tetanic stimuli (60 s) a time-dependent tension decay was observed. This decay was prolonged by reducing the extracellular K+ from 5.4 to 1.0 microM, suggesting that Ca2+ extrusion through the Na-Ca exchanger seems to occur during tetanic stimulation. Since tetanic tension was never higher than the tension obtained in twitches elicited at the same Ca2+ concentration (0.5 Hz), we conclude that tetanic contractures represent a useful tool to investigate the contractile response of intact myocardial preparations with a nonfunctional sarcoplasmic reticulum.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Physiological Sciences, Federal University of Espírito Santo, Vitória, Brazil.
authors.forename: C M
authors.initials: CM
authors.lastname: Leite
authors.forename: D V
authors.initials: DV
authors.lastname: Vassallo
authors.forename: J G
authors.initials: JG
authors.lastname: Mill
mesh_terms: D000818:Animals; D002110:Caffeine / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006321:Heart / Q000187:drug effects*; D007474:Ion Exchange; D008297:Male; D009119:Muscle Contraction; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012519:Sarcoplasmic Reticulum / Q000187:drug effects*; D013997:Time Factors
chemical_list: D002110:Caffeine; D011189:Potassium Chloride; D002118:Calcium