Evaluation of the micronucleus test in vitro using Chinese hamster cells: results of four chemicals weakly positive in the in vivo micronucleus test.
A rapid and simple procedure for the micronucleus test (MNT) in vitro using Chinese hamster ovary (CHO) cells was established in our laboratory. The assay is intended to quickly screen chromosomal aberrations in vitro within the framework of industrial genotoxicity studies. To test the sensitivity of the assay in the experiments described here, four substances, classified as noncarcinogens but reported as weak inducers of micronuclei (MN) in bone-marrow cells of mice, were evaluated in the MNT in vitro. Of the four compounds, ascorbic acid, phenol, and 2,6-diaminotoluene proved to be genotoxic in the MNT in vitro. Titanium dioxide, which could not be dissolved in the culture medium, did not induce MN. The MNT in vitro proved to be quick and relatively simple and to yield highly reproducible results when testing the four chemicals.
Miller
B M
BM
Pharma Division, Department of Toxicology, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
Pujadas
E
E
Gocke
E
E
D000818:Animals; D001205:Ascorbic Acid / Q000633:toxicity*; D001761:Bleomycin / Q000633:toxicity; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D003520:Cyclophosphamide / Q000633:toxicity; D051379:Mice; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D019800:Phenol; D010636:Phenols / Q000633:toxicity*; D010655:Phenylenediamines / Q000633:toxicity*; D015203:Reproducibility of Results; D014025:Titanium / Q000633:toxicity*
D009153:Mutagens; D010636:Phenols; D010655:Phenylenediamines; D001761:Bleomycin; C009495:titanium dioxide; D019800:Phenol; D003520:Cyclophosphamide; D014025:Titanium; C025235:2,6-diaminotoluene; D001205:Ascorbic Acid