Immunoreactivity for c-fos and c-myc protein with the monoclonal antibodies 14E10 and 6E10 in malignant mesothelioma and non-neoplastic mesothelium of the pleura.
We studied immunoreactivity for c-fos protein and c-myc protein in malignant mesothelioma (36 cases) and non-neoplastic pleural mesothelium (45 cases) using the murine monoclonal antibodies 14E10 and 6E10. All malignant mesotheliomas and cases with non-neoplastic mesothelium exhibited not only nuclear but also cytoplasmic immunoreactivity for c-fos and c-myc protein in the majority of mesothelial cells. There was no statistically significant difference between the various mesothelioma subtypes or between neoplastic and non-neoplastic mesothelium for c-fos protein immunoreactivity (p > 0.05). There was statistically significant difference between neoplastic and non-neoplastic mesothelium for c-myc protein immunoreactivity (p < 0.01). We conclude that immunoreactivity for c-fos and c-myc protein is present in both non-neoplastic and neoplastic mesothelium, but that a higher proportion of neoplastic mesothelial cells are immunoreactive for c-myc protein when compared with non-neoplastic mesothelium.
Ramael
M
M
Laboratory of Pathology, University Hospital Antwerp, Edegem, Belgium.
Van den Bossche
J
J
Buysse
C
C
Deblier
I
I
Segers
K
K
Van Marck
E
E
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008654:Mesothelioma / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D008969:Molecular Sequence Data; D016612:Paraffin Embedding; D010995:Pleural Diseases / Q000378:metabolism* / Q000473:pathology; D010997:Pleural Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D016760:Proto-Oncogene Proteins c-fos; D016271:Proto-Oncogene Proteins c-myc