Mapping of beta-1,2-linked oligomannosidic epitopes among glycoconjugates of Candida species.
The distribution of beta-1,2-linked oligomannosides among glycoconjugates of various Candida species was investigated by Western blotting, using monoclonal and polyclonal antibodies which react with these epitopes. Expression of beta-1,2-linked oligomannosidic epitopes on a 14-18 kDa polydisperse antigen nonreactive with concanavalin A (ConA), previously identified as a C. albicans serotype A phospholipomannan (PLM), appeared to be restricted to C. albicans serotypes A and B (including var. C. stellatoidea types I and II) and C. tropicalis. In C. albicans, beta-1,2-linked oligomannosidic epitopes also appeared to be slightly associated with high molecular mass (> 100 kDa) polydisperse ConA-reactive mannoproteins. For all the other Candida strains investigated, belonging to the species C. parapsilosis, C. krusei, C. glabrata and C. robusta (S. cerevisiae), beta-1,2-linked oligomannosidic epitopes were found to be present in association with medium molecular mass (18-100 kDa) and high molecular mass ConA-reactive mannoproteins, giving reproducible labelling profiles that varied between species.
Cantelli
C
C
Unité 42, Institut National de la Santé et de la Recherche Médicale, Domaine du CERTIA, Villeneuve d'Ascq, France.
Trinel
P A
PA
Bernigaud
A
A
Jouault
T
T
Polonelli
L
L
Poulain
D
D
D000908:Antibodies, Fungal; D015153:Blotting, Western; D002175:Candida / Q000254:growth & development / Q000276:immunology*; D002176:Candida albicans / Q000276:immunology; D000939:Epitopes; D006001:Glycoconjugates / Q000276:immunology*; D008362:Mannosides / Q000276:immunology*; D009844:Oligosaccharides / Q000276:immunology*; D012703:Serotyping; D013045:Species Specificity
D000908:Antibodies, Fungal; D000939:Epitopes; D006001:Glycoconjugates; D008362:Mannosides; D009844:Oligosaccharides; C028569:oligomannoside