A time-related study of Brefeldin A effects in HSV-1 infected cultured human fibroblasts.
Glycoprotein D (gD-1) is an essential virion envelope component of herpes simplex virus type 1 (HSV-1) normally transported to the plasma membrane of the infected cells. In the present study, the intracellular transport of gD-1 was inhibited in cultured HSV-1 infected human fibroblasts by Brefeldin A (BFA) 1 microgram/ml medium added for 12 h after virus adsorption. Immunofluorescence light- and confocal microscopy revealed abolished transport of gD-1 to the plasma membrane, juxtanuclear accumulation of gD-1, and a disorderly arrangement of the tubulin fibres. Withdrawal of BFA influence for more than 60 min resulted in incomplete transport but increasing accumulation of gD-1 in the plasma membrane and in Golgi-like areas close to the nuclei. The tubulin pattern was almost normalized 6 h after removal of BFA. The egress of infectious HSV-1 particles released 9 h post-BFA treatment was not fully reestablished. The results indicate that BFA effects were not completely reversible and caused a sort of cytotoxic influence involving the structure of tubulin.
Jensen
H L
HL
Institute of Medical Microbiology and Immunology, University of Copenhagen, Panum Institute, Denmark.
Rygaard
J
J
Norrild
B
B
D000998:Antiviral Agents / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D020126:Brefeldin A; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000494:pharmacology*; D005347:Fibroblasts / Q000821:virology*; D006561:Herpes Simplex / Q000188:drug therapy / Q000503:physiopathology; D006801:Humans; D018613:Microscopy, Confocal; D018139:Simplexvirus / Q000187:drug effects / Q000502:physiology*; D013995:Time; D014759:Viral Envelope Proteins / Q000502:physiology*
D000998:Antiviral Agents; D003517:Cyclopentanes; D014759:Viral Envelope Proteins; D020126:Brefeldin A