title: Postmortem diffusion of alcohol from the stomach.
abstract: We explored postmortem ethanol diffusion from the stomach using a human cadaver model with multiple blood site sampling. In all, 400 ml of alcohol solution (5%, 10%, 20%, or 40% methanol and ethanol weight/volume in water) was introduced into the stomach by oesophageal tube. Blood methanol concentrations correlated with ethanol concentrations (methanol range, 1-676 mg%; ethanol range, 1-531 mg%; r = 0.9973). The pattern of ethanol diffusion showed marked between-case variability. Typically, concentrations were highest in pericardial fluid and, in decreasing order, in left pulmonary vein, aorta, left heart, pulmonary artery, superior vena cava, inferior vena cava, right heart, right pulmonary vein, and femoral vein. Diffusional flux was broadly proportional to the concentration of ethanol used. It was time-dependent (as assessed by 24-h and 48-h sampling) and markedly inhibited by refrigeration at 4 degrees C. After gastric instillation of 400 ml of 5% solution for 48 h at room temperature in paired cadavers, ethanol concentrations (mg%) were as follows: pericardial fluid 135, 222; aorta 50, 68; left heart 77, 26; right heart 41, 28; femoral vein 0. Using a 10% solution, ethanol concentrations (mg%) were as follows: pericardial fluid 401, 255; aorta 129, 134; left heart 61, 93; right heart 31, 41; femoral vein 5, 7. Introducing 50 ml of 10% alcohol solution into the oesophagus after oesophagogastric junction ligation produced similar aortic blood ethanol concentrations. This suggests that postmortem gastrooesophageal reflux and diffusion from the oesophagus is the mechanism of artefactual elevation of aortic blood ethanol.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Forensic Medicine, University of Dundee, Scotland.
authors.forename: D J
authors.initials: DJ
authors.lastname: Pounder
authors.forename: D R
authors.initials: DR
authors.lastname: Smith
mesh_terms: D001011:Aorta / Q000737:chemistry; D002102:Cadaver; D004058:Diffusion; D000431:Ethanol / Q000097:blood* / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D010496:Pericardium / Q000737:chemistry; D011180:Postmortem Changes; D011667:Pulmonary Veins / Q000737:chemistry; D013270:Stomach / Q000473:pathology; D014682:Vena Cava, Inferior / Q000737:chemistry
chemical_list: D000431:Ethanol