GCAP-II: isolation and characterization of the circulating form of human uroguanylin.
The systematic isolation of circulating regulatory peptides which generate cGMP as second messenger resulted in the identification of a novel member of the guanylin family. In the present study we describe the purification and amino acid sequence of a new guanylate cyclase C activating peptide (GCAP-II). GCAP-II contains 24 amino acids in the following sequence: FKTLRTIANDDCELCVNVACTGCL. Its molecular mass is 2597.7 Da. The 16 C-terminal amino acids are identical to uroguanylin from human urine. native and synthetic GCAP-II activate GC-C, the specific guanylate cyclase receptor, of cultured human colon carcinoma (T84) cells. GCAP-II stimulates chloride secretion in isolated human intestinal mucosa mediated by intracellular cGMP increase. GCAP-II specific antibodies were used to localize the peptide by immunohistochemistry in entero-endocrine cells of the colonic mucosa.
Hess
R
R
Lower Saxony Institute for Peptide Research (IPF), Hannover, Germany.
Kuhn
M
M
Schulz-Knappe
P
P
Raida
M
M
Fuchs
M
M
Klodt
J
J
Adermann
K
K
Kaever
V
V
Cetin
Y
Y
Forssmann
W G
WG
D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification* / Q000502:physiology; D006152:Cyclic GMP / Q000378:metabolism; D051604:Guanylate Cyclase-Activating Proteins; D006801:Humans; D008969:Molecular Sequence Data; D045265:Natriuretic Peptides; D010455:Peptides / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
D002135:Calcium-Binding Proteins; C496739:GUCA1B protein, human; D051604:Guanylate Cyclase-Activating Proteins; D045265:Natriuretic Peptides; D010455:Peptides; C084362:uroguanylin; D006152:Cyclic GMP