Heat shock proteins in the human periodontal disease process.
The production of HSP by periodontopathic Gram-negative bacteria was examined by SDS-PAGE, two dimensional gel electrophoresis, and Western blotting using monoclonal antibodies against HSPs. Strains of Actinobacillus actinomycetemcomitans, Eikenella corrodens, Fusobacterium nucleatum, Prevotella intermedia, Prevotella nigrescens, Prevotella melaninogenica, and Treponema socranskii species produced HSP which reacted with anti-Yersinia enterocolitica HSP 60 and/or mycobacterial 65-kDA HSP monoclonal antibodies. It found that gingival homogenate samples from patients with adult periodontitis reacted with anti-human HSP were also found in a serum sample from a periodontitis patient. The present study suggests that HSPs are implicated in human periodontal disease process.
Ando
T
T
Department of Oral and Maxillofacial Surgery, Tokyp Women's College, Japan.
Kato
T
T
Ishihara
K
K
Ogiuchi
H
H
Okuda
K
K
D000328:Adult; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins; D015153:Blotting, Western; D001923:Brain Chemistry; D002417:Cattle; D018834:Chaperonin 60 / Q000276:immunology; D018833:Chaperonins / Q000276:immunology; D003429:Cross Reactions; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005881:Gingiva / Q000382:microbiology; D006090:Gram-Negative Bacteria / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000276:immunology; D006360:Heat-Shock Proteins / Q000032:analysis* / Q000276:immunology; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000276:immunology; D010518:Periodontitis / Q000097:blood / Q000382:microbiology*; D013045:Species Specificity
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D018834:Chaperonin 60; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins