Genotyping of rotaviruses isolated from sewage.
Rotaviruses from environmental samples have been genotyped by a seminested reverse transcription PCR assay with serotype-specific primers derived from variable regions of gene 9, which produce different characteristic segment sizes for serotypes 1 to 4. The method enabled the detection and identification of type 1, 2, and 3 group A rotaviruses in sewage.
Gajardo
R
R
Department of Microbiology, University of Barcelona, Spain.
Bouchriti
N
N
Pintó
R M
RM
Bosch
A
A
D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012703:Serotyping; D012722:Sewage; D014871:Water Microbiology
D012722:Sewage