Effects of leukemia inhibitory factor on the differentiation of astrocyte progenitor cells from embryonic mouse cerebral hemispheres.
The effects of leukemia inhibitory factor (LIF) on the differentiation and proliferation of astrocyte progenitor cells in an enriched culture were studied. The astrocyte progenitor cells obtained from embryonic mouse cerebral hemispheres were enriched by subculturing twice in serum-free medium containing epidermal growth factor (EGF). They exhibited a flat, non-process-bearing morphology and were negative for glial fibrillary acidic protein (GFAP), a specific marker for astrocytes. Culture of the cells with LIF resulted in the expression of GFAP and in the inhibition of growth. These results suggest that LIF promotes the differentiation of astrocyte progenitor cells.
Nakagaito
Y
Y
Institute for Fermentation Osaka (IFO), Japan.
Yoshida
T
T
Satoh
M
M
Takeuchi
M
M
D000818:Animals; D001253:Astrocytes / Q000166:cytology* / Q000378:metabolism; D001921:Brain / Q000196:embryology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006131:Growth Inhibitors / Q000494:pharmacology*; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D013997:Time Factors
D005904:Glial Fibrillary Acidic Protein; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines