Production in rabbits of high levels of anti-HIV-1 gp160 antibodies.
The production of anti-HIV-1 gp160 antibodies was obtained in rabbits given gp160 either in saline or adsorbed onto calcium phosphate. Immunization with gp160 in saline induced the formation of antibodies directed to the p18 protein, whereas the gp160 adsorbed onto calcium phosphate elicited antibodies recognizing the gp160, p55, p25 and p18 proteins. Calcium phosphate was found to be a powerful adjuvant and it should be used for potentiating candidate anti-HIV vaccines.
Relyveld
E
E
Institut Pasteur, Unité des Vaccins Bactériens, Marnes-La-Coquette, France.
Bizzini
B
B
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D015153:Blotting, Western; D002130:Calcium Phosphates / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D015686:Gene Products, env / Q000276:immunology*; D015683:Gene Products, gag / Q000276:immunology; D015483:HIV Antibodies / Q000096:biosynthesis*; D019302:HIV Envelope Protein gp160; D015497:HIV-1 / Q000276:immunology; D008297:Male; D011498:Protein Precursors / Q000276:immunology*; D011817:Rabbits; D015531:Radioimmunoprecipitation Assay; D013997:Time Factors; D014611:Vaccination
D000276:Adjuvants, Immunologic; D002130:Calcium Phosphates; D015686:Gene Products, env; D015683:Gene Products, gag; D015483:HIV Antibodies; D019302:HIV Envelope Protein gp160; D011498:Protein Precursors; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous