title: Cytochrome b-558 alpha-subunit cloning and expression in rat aortic smooth muscle cells.
abstract: Recent studies have shown that the NADPH oxidase participates in the generation of superoxide anion in non-phagocytic cells. Here we report the isolation and nucleotide sequence of a cDNA for the cytochrome b-558 alpha-subunit of the NADPH oxidase in rat vascular smooth muscle cells (VSMCs). The coding region of the cDNA was 93% homologous to mouse and 81% to human in nucleotide sequence and 96% homologous to mouse and 89% to human in the deduced amino acid sequence. Our results provide a tool with which to explore the mechanism of superoxide anion generation in rat VSMCs and other non-phagocytic cells.
authors.affiliation: Department of Medicine, Emory University, Atlanta, GA 30322, USA.
authors.forename: T
authors.initials: T
authors.lastname: Fukui
authors.forename: B
authors.initials: B
authors.lastname: Lassègue
authors.forename: H
authors.initials: H
authors.lastname: Kai
authors.forename: R W
authors.initials: RW
authors.lastname: Alexander
authors.forename: K K
authors.initials: KK
authors.lastname: Griendling
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000737:chemistry* / Q000166:cytology; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D003573:Cytochrome b Group / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000737:chemistry* / Q000166:cytology; D019255:NADPH Oxidases; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid
chemical_list: D003573:Cytochrome b Group; D018076:DNA, Complementary; C036888:cytochrome b558; D019255:NADPH Oxidases