[Antitumor effect of intratumoral administration of BRM: inhibition of tumor cell invasion in vitro].
The effect of intratumoral administration of BRMs in Meth-A solid tumor has been analyzed in BALB/c mice. The effect of BRMs on in vitro invasion by murine RL male-1 leukemia cells was studied using Biocoat Matrigel Invasion Chamber (Becton Dickinson Labware). We determined the ability of tumor cells to penetrate matrigel-coated filters in the presence or absence of BRM. PSK or OK-432 inhibited tumor cell invasion of matrigel-coated filters in a dose-dependent manner. PSK, OK-432 and Cepharanthin inhibited invasion of murine Colon 26 carcinoma cells and human A 375. S2 melanoma cells. On the other hand, polysaccharide preparations without protein, Lentinan or Sonifilan inhibited neither tumor growth nor tumor cell invasion.
Ebina
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Division of Immunology, Miyagi Cancer Center.
Murata
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K
D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D015552:Injections, Intralesional; D007942:Leukemia, Experimental / Q000473:pathology; D008297:Male; D008546:Melanoma, Experimental / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D010844:Picibanil / Q000494:pharmacology; D011509:Proteoglycans / Q000494:pharmacology; D012513:Sarcoma, Experimental / Q000473:pathology; D014407:Tumor Cells, Cultured
D007155:Immunologic Factors; D011509:Proteoglycans; D010844:Picibanil; C010770:polysaccharide-K