Substrate specificity and the use of chitosan-entrapped rabbit hepatic microsomal UDP-glucuronyl transferase for detoxification.
Rabbit hepatic microsomal UDP-Glucuronyl transferase (EC.2.4.1.17) was immobilized in chitosan, which is an ionotropic gelatation agent, and the resulting preparation was used as a biocatalyst for the glucuronidation of toxic substances and drugs such as; 1-naphthol, phenol, 4-nitrophenol, bilirubin, testesterone, estrone, lamotrigine, imipramine and chlorpromazine. For studying the drug metabolism in vitro and obtaining artificial liver support (detoxification) chitosan-entrapped UDPGT towards 1-naphthol was tested by using two model reactor system. The conversion curves of 1-naphthol to its glucuronide indicated that the column reactor system was found to be better and seems suitable for detoxification with a high yield of glucuronide formation.
Zihnioğlu
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Ege University, Faculty of Science, Biochemistry Department, Bornova-Izmir, Türkiye.
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D000818:Animals; D002686:Chitin / Q000031:analogs & derivatives; D048271:Chitosan; D004800:Enzymes, Immobilized; D014453:Glucuronosyltransferase / Q000378:metabolism*; D008658:Inactivation, Metabolic; D008862:Microsomes, Liver / Q000201:enzymology*; D011817:Rabbits; D013379:Substrate Specificity
D004800:Enzymes, Immobilized; D002686:Chitin; D048271:Chitosan; D014453:Glucuronosyltransferase