Genes encoding the group I intron-containing tRNA(Leu) and subunit L of NADH dehydrogenase from the cyanobacterium Synechococcus PCC 6301.
A part of the tRNA(Leu)(UAA) gene containing a 240-nucleotide group I intron was amplified by PCR from cyanobacterium Synechococcus PCC 6301 genomic DNA. The pre-tRNA synthesized from the cloned PCR product was efficiently self-spliced in vitro under physiological conditions. The gene encoding the tRNA(Leu)(UAA), trnL-UAA, was isolated from a Synechococcus PCC 6301 genomic library and the nucleotide sequence of a 2,167-bp portion was determined. The trnL-UAA consists of a 34-bp 5' exon, a 240-bp group I intron and a 50-bp 3' exon. In addition, three open reading frames (ORF1, ORF2 and ORF3) were found in the 5' and 3' flanking regions of trnL-UAA. The predicted protein sequence of ORF3, which is located 74-bp upstream from trnL-UAA on the opposite strand, shows 66.2% amino acid identity to that of the Synechocystis PCC 6803 gene encoding subunit L of NADH dehydrogenase (ndhL).
Sugita
M
M
Center for Gene Research, Nagoya University, Japan.
Luo
L
L
Ohta
M
M
Itadani
H
H
Matsubayashi
T
T
Suguira
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D000458:Cyanobacteria / Q000201:enzymology / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D016680:Genome, Bacterial; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012356:RNA, Transfer, Leu / Q000235:genetics*; D012641:Selection, Genetic
D004269:DNA, Bacterial; D012356:RNA, Transfer, Leu; D009245:NADH Dehydrogenase