title: Cellular composition of primary cultures of human granulosa-lutein cells and the effect of cytokines on cell proliferation.
abstract: The cellular composition of cells collected from the follicular fluid obtained during the IVF procedure and cultured in vitro was examined, as well as the effects of two cytokines, interleukin-2 (IL-2) and tumour necrosis factor-alpha (TNF alpha), on the proliferation of individual cell types. After 48 h in culture, most of the cells were granulosa-lutein cells exhibiting positive staining against 3-beta-hydroxysteroid dehydrogenase (3 beta-HSD). About one-third of the total cell population stained positive with monoclonal antibodies against specific antigen sites on lymphohaemopoietic cells, including B-lymphocytes, T-lymphocytes, natural killer cells, monocytes and macrophages. During this 48-h period, IL-2 and TNF alpha significantly (P < 0.05) increased the proportion of leukocyte common antigen (LCA)-positive cells and monocytes. At the end of 144 h in culture, although LCA-positive cells and monocytes were still present, there were fewer. The main targets for the proliferative effects of IL-2 and TNF alpha in this culture system during the first 48 h are leukocytes rather than steroid-producing cells. Thus, any observed effects from the addition of cytokines in this system may be due to indirect effects of cytokine-activated leukocytes on granulosa-lutein cells.
authors.affiliation: Reproductive Medicine Unit, Queen Elizabeth Hospital, Woodville, Australia.
authors.forename: L J
authors.initials: LJ
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Brännström
authors.forename: V
authors.initials: V
authors.lastname: Pascoe
authors.forename: R J
authors.initials: RJ
authors.lastname: Norman
mesh_terms: D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008184:Luteal Cells / Q000166:cytology* / Q000187:drug effects*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D009000:Monocytes / Q000166:cytology / Q000187:drug effects; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha