Steroidal saponins from the underground parts of Hosta longipes and their inhibitory activity on tumor promoter-induced phospholipid metabolism.
Phytochemical study on the underground parts of Hosta longipes gave six new steroidal saponins together with a known one. The structures of the new compounds were determined by detailed analysis of their 1H- and 13C-NMR spectra including two-dimensional NMR spectroscopy, acid-catalyzed hydrolysis followed by chemical correlation, and by comparison with spectral data of known compounds. The isolated saponins and their aglycones were examined for inhibitory activity on 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated 32P-incorporation into phospholipids of HeLa cells to identify new antitumor-promoter compounds.
Mimaki
Y
Y
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
Kanmoto
T
T
Kuroda
M
M
Sashida
Y
Y
Nishino
A
A
Satomi
Y
Y
Nishino
H
H
D016588:Anticarcinogenic Agents / Q000737:chemistry* / Q000494:pharmacology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D006367:HeLa Cells; D006801:Humans; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D010944:Plants / Q000737:chemistry*; D011522:Protons; D012503:Saponins / Q000737:chemistry* / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity
D016588:Anticarcinogenic Agents; D002247:Carbon Isotopes; D010743:Phospholipids; D010936:Plant Extracts; D011522:Protons; D012503:Saponins; D013755:Tetradecanoylphorbol Acetate