Influence of various concentrations of taxol on cell survival, micronuclei induction, and LDH activity in cultured V79 cells.
The clastogenic effect of various concentrations of taxol, namely 0, 10, 25, 50, 100, 250 and 500 nM was evaluated in V79 cells grown in vitro. Treatment of V79 cells with different concentrations of taxol reduced the survival in a concentration dependent manner. However, the frequency of micronuclei increased in a concentration dependent manner and the dose response was linear quadratic. Similarly, lactate dehydrogenase also increased with the increase in taxol concentration. The micronuclei and lactate dehydrogenase showed an inverse relationship with the cell survival.
Jagetia
G C
GC
Department of Radiobiology, Kasturba Medical College, Manipal, India.
Adiga
S K
SK
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000633:toxicity*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D003114:Colony-Forming Units Assay; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D017239:Paclitaxel / Q000633:toxicity*
D000972:Antineoplastic Agents, Phytogenic; D009153:Mutagens; D007770:L-Lactate Dehydrogenase; D017239:Paclitaxel