Catabolite repression of the adhesion of Vero cytotoxin-producing Escherichia coli of serogroups O157 and O111.
The virulence traits that mediate Vero cytotoxin-producing E. coli (VTEC) adherence are unclear. Many VTEC strains possess the eaeA gene which is involved in the attaching and effacing effects of enteropathogenic E. coli (EPEC). Most eae-positive VTEC adhered to HEp-2 cells in a localized manner; however some strains did not adhere. Thus we investigated the adhesion of poorly adherent strains, especially those of serogroups O111 and O157. To establish a model, the adherence to HEp-2, INT407 and Caco-2 cells of 12 O157 VTEC and six O111 VTEC isolated from cases of human infection were studied after growth of the bacteria under different conditions. For adhesion tests mannose is usually added during prior broth culture of the bacteria, and during the period of attachment, so that any adhesion due to mannose-sensitive type 1 pili is inhibited. Bacteria cultured in peptone water in the absence of mannose adhered to all three lines; there were localized clusters of bacteria on 1%-82% cells, whether mannose was present during the attachment period or not. Bacteria grown in the presence of D-mannose, or any other sugar that was metabolized, showed little adherence (range 0-9%). alpha-Methyl-glucoside also caused marked inhibition of adhesion. It was concluded that inhibition of adhesion was due to catabolite repression.
Nishikawa
Y
Y
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK.
Scotland
S M
SM
Smith
H R
HR
Willshaw
G A
GA
Rowe
B
B
D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002241:Carbohydrates / Q000494:pharmacology; D002460:Cell Line; D000242:Cyclic AMP / Q000494:pharmacology; D004926:Escherichia coli / Q000145:classification / Q000472:pathogenicity / Q000502:physiology*; D005798:Genes, Bacterial; D006801:Humans; D008358:Mannose / Q000494:pharmacology; D008757:Methylglucosides / Q000494:pharmacology; D012703:Serotyping; D022622:Shiga Toxin 1; D014774:Virulence / Q000235:genetics
D001427:Bacterial Toxins; D002241:Carbohydrates; D008757:Methylglucosides; D022622:Shiga Toxin 1; C027020:methylglucoside; D000242:Cyclic AMP; D008358:Mannose