Mechanisms of 3-carene-induced bronchoconstriction in the isolated guinea pig lung.
Inhaled 3-carene at a concentration of 5,000 mg/m3 caused bronchoconstriction in isolated, ventilated and perfused guinea pig lungs. This effect was inhibited by the cyclooxygenase inhibitor diclofenac (100 microM) and the thromboxane/prostaglandin endoperoxide-receptor antagonist L-670,596 (1 microM). 3-Carene exposure also increased the amount of thromboxane in the perfusate from the lungs. In cultured calf pulmonary arterial endothelial cells 3-carene caused a dose-related release of arachidonic acid. Thus, the results obtained in this experimental model may have implications in the understanding of the pathophysiology of 3-carene-induced obstructive pulmonary disease in humans.
Låstbom
L
L
Division of Toxicology, Karolinska Institute, Stockholm, Sweden.
Falk-Filipsson
A
A
Boyer
S
S
Moldéus
P
P
Ryrfeldt
A
A
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D000081008:Bicyclic Monoterpenes; D016084:Bronchoconstriction / Q000187:drug effects*; D002227:Carbazoles / Q000494:pharmacology; D002417:Cattle; D002478:Cells, Cultured; D004008:Diclofenac / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004727:Endothelium / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008168:Lung / Q000187:drug effects*; D008297:Male; D039821:Monoterpenes; D011448:Prostaglandin Antagonists / Q000494:pharmacology; D011651:Pulmonary Artery / Q000166:cytology / Q000378:metabolism; D015656:Respiratory Mechanics / Q000187:drug effects; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000096:biosynthesis; D013997:Time Factors
D000081008:Bicyclic Monoterpenes; D002227:Carbazoles; D039821:Monoterpenes; D011448:Prostaglandin Antagonists; D013729:Terpenes; C061299:L 670596; D004008:Diclofenac; D016718:Arachidonic Acid; D013929:Thromboxane B2; C030218:3-carene