title: A second-generation YAC contig map of human chromosome 3.
abstract: A map of human chromosome 3 which integrates both physical and genetic data has been developed from the fusion of two large collections of markers and corresponding yeast artificial chromosome (YAC) clones. The map contains 972 megabase-sized YACs identified with 593 primary markers, of which 162 are highly polymorphic sequence-tagged sites (STSs) and form a closely spaced genetic linkage map; the remaining markers are hybridization-based. Chromosome 3 is now represented by 24 large YAC contigs whose order and orientation is largely known. The map generated by fusion of these hybridization- and STS-based datasets covers about 80% (over 160 megabases) of the chromosome and will provide the foundation necessary for rapid development of a detailed genetic understanding for this large autosome.
authors.affiliation: Eleanor Roosevelt Institute, Denver, Colorado 80206, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Gemmill
authors.forename: I
authors.initials: I
authors.lastname: Chumakov
authors.forename: P
authors.initials: P
authors.lastname: Scott
authors.forename: B
authors.initials: B
authors.lastname: Waggoner
authors.forename: P
authors.initials: P
authors.lastname: Rigault
authors.forename: J
authors.initials: J
authors.lastname: Cypser
authors.forename: Q
authors.initials: Q
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Weissenbach
authors.forename: K
authors.initials: K
authors.lastname: Gardiner
authors.forename: H
authors.initials: H
authors.lastname: Wang
mesh_terms: D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002893:Chromosomes, Human, Pair 3; D016208:Databases, Factual; D005819:Genetic Markers; D006801:Humans; D016324:Sequence Tagged Sites; D012984:Software
chemical_list: D005819:Genetic Markers