Characteristic biological features of human megakaryoblastic leukaemia cell lines.
Both normal and leukaemic human megakaryocytopoiesis are stimulated by several cytokines, including stem cell factor, granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin-3, GM-CSF/interleukin-3 fusion protein, interleukin-6, interleukin-11, basic fibroblast growth factor and thrombopoietin, but are inhibited by tumour necrosis factor-alpha, platelet factor 4, beta-thromboglobulin, thrombin, interleukin-4, interferon-alpha and interferon-gamma. Human megakaryoblastic leukaemia cell lines have common biological features, including high expression of the megakaryocytic specific antigen: CD41; high expression of the early myeloid antigens: CD34 and CD33; constitutive expression of interleukin-6 and platelet-derived growth factor; complex karyotype picture; expression of c-kit: the stem cell factor receptor; growth-dependency or -stimulation by stem cell factor, interleukin-3 and/or GM-CSF; megakaryoblastic differentiation by phorbol-myristate-acetate; and in vivo tumorigenicity in mice is associated with marked fibrosis. Only a few agents including phorbol-myristate-acetate; vitamin D3, interferon-alpha, interferon-beta 2, erythropoietin and thrombin have been reported to induce megakaryocytic differentiation in the human megakaryoblastic leukaemia cells.
Hassan
H T
HT
Department of Haematology and Oncology, University of Hannover Medical School, Germany.
Freund
M
M
D000328:Adult; D000368:Aged; D002454:Cell Differentiation; D002455:Cell Division; D016207:Cytokines / Q000494:pharmacology; D005260:Female; D006410:Hematopoiesis; D006801:Humans; D007223:Infant; D007947:Leukemia, Megakaryoblastic, Acute / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D008533:Megakaryocytes / Q000166:cytology*; D008875:Middle Aged; D014407:Tumor Cells, Cultured
D016207:Cytokines