Effects of overexpressing folding modulators on the in vivo folding of heterologous proteins in Escherichia coli.
Interest continues to increase in the use of folding modulators to overcome problems with heterologous protein folding in Escherichia coli. Currently, this approach, though highly successful with a number of individual proteins, remains a somewhat hit-and-miss affair. Ongoing research directed at unraveling the precise role and specificity of these folding modulators should generate a clearer understanding of the potential and limitations of overexpressing folding catalysts in vivo. This will facilitate the development, in the not too distant future, of a more structured and rational approach to improving the folding of heterologous gene products in E. coli.
Wall
J G
JG
University of Zürich, Switzerland.
Plückthun
A
A
D010450:Endopeptidases / Q000172:deficiency; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007536:Isomerism; D018832:Molecular Chaperones; D015202:Protein Engineering; D017510:Protein Folding; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism
D018832:Molecular Chaperones; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D010450:Endopeptidases