Developments in expression cloning.
Recent years have seen a dramatic expansion in the range of applications of expression cloning techniques. New vectors and detection methods promise to further broaden the applicability of function-based screening approaches to problems in gene discovery. A major theme in the past year has been the introduction of engineered reporter cells that heighten the sensitivity with which clones expressing cDNAs can be identified.
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B
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Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA.
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D015870:Gene Expression; D008322:Mammals; D011387:Prokaryotic Cells; D011994:Recombinant Proteins / Q000096:biosynthesis
D011994:Recombinant Proteins