[Experimental infection of infant rabbits with verocytotoxin-producing Escherichia coli of bovine origin].
Infant rabbits were used as a model to study the diarrhogenicity of verocytotoxin-producing Escherichia coli (VTEC) isolated from bovine. Infant rabbits aged 6 days were inoculated intragastrically with 10(9) viable bacteria of four VTEC or three non-VTEC strains. Of these strains, three strains (VT+, eaeA+), one strain (VT+, eaeA-) and one strain (VT-, eaeA+) caused diarrhea in rabbits 48 to 60 hr after inoculation. None of the two strains (VT-, eaeA-) caused symptoms. Based on these results, it is suggested that the 6-day-old infant rabbit is a suitable animal for studying diarrhea caused by VTEC or eaeA-positive E. coli strains of bovine origin.
Nakazawa
M
M
Kyushu Branch Laboratory, National Institute of Animal Health.
Itoh
K
K
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000096:biosynthesis*; D001483:Base Sequence; D002417:Cattle; D003967:Diarrhea / Q000382:microbiology*; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits; D022622:Shiga Toxin 1
D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D022622:Shiga Toxin 1