title: The use of isoelectric focusing on polyacrylamide gel for the enzymatic analysis of 'Old World' Leishmania species.
abstract: Using isoelectric focusing (IEF), a high resolution electrophoresis technique, we analysed 6 enzymes of 24 cloned strains representing all major taxa of 'Old World' Leishmania. The comparison of enzymatic patterns obtained with IEF and starch gel electrophoresis showed that IEF is a more discriminatory and more informative technique for the enzymatic analysis of Leishmania strains; it can detect very slight differences between 2 electromorphs not revealed with starch gel electrophoresis. Moreover, IEF detected several multi-banded patterns which appeared as single bands with starch gel electrophoresis. These multi-banded patterns could not be the result of strain heterogeneity since all the strains had been cloned. Their significance in the biology of Leishmania is discussed.
authors.affiliation: Laboratoire d'Ecologie Médicale et Pathologie Parasitaire, Faculté de Médecine, Université de Montpellier, France.
authors.forename: R
authors.initials: R
authors.lastname: Piarroux
authors.forename: V
authors.initials: V
authors.lastname: Trouvé
authors.forename: F
authors.initials: F
authors.lastname: Pratlong
authors.forename: A
authors.initials: A
authors.lastname: Martini
authors.forename: M
authors.initials: M
authors.lastname: Lambert
authors.forename: J A
authors.initials: JA
authors.lastname: Rioux
mesh_terms: D000818:Animals; D004592:Electrophoresis, Starch Gel; D007525:Isoelectric Focusing / Q000379:methods; D007527:Isoenzymes / Q000032:analysis; D007891:Leishmania / Q000145:classification* / Q000201:enzymology
chemical_list: D007527:Isoenzymes