Direct demonstration of sulphonylurea-sensitive KATP channels on nerve terminals of the rat motor cortex.
We examined whether ATP-sensitive potassium (KATP) channels are present on presynaptic terminals of the rat motor cortex, an area of the CNS exhibiting a high density of sulphonylurea binding. A novel fused nerve terminal preparation was developed which produced structures amenable to patch clamp methods. In inside-out recordings a K+ channel was observed which possessed all the major features of the Type 1 KATP channel, including sensitivity to ATP and the antidiabetic sulphonylureas.
Lee
K
K
Department of Pharmacology, University of Cambridge.
Dixon
A K
AK
Rowe
I C
IC
Ashford
M L
ML
Richardson
P J
PJ
D000110:Acetylcholinesterase / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004558:Electric Stimulation; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D009411:Nerve Endings / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects*; D017729:Presynaptic Terminals / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013453:Sulfonylurea Compounds / Q000378:metabolism / Q000494:pharmacology*
D015221:Potassium Channels; D011506:Proteins; D013453:Sulfonylurea Compounds; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase; D002795:Choline O-Acetyltransferase; D002950:Citrate (si)-Synthase; D000110:Acetylcholinesterase