Liquid chromatographic determination of simazine, atrazine, and propazine residues in catfish.
A liquid chromatographic (LC) method is described for the simultaneous determination of the triazine herbicides, simazine (SIM), atrazine (ATZ), and propazine (PRO) in the 12.5-100 ppb range in catfish. The herbicides are extracted from catfish homogenates with ethyl acetate, followed by solvent partitioning between acetonitrile and petroleum ether and additional cleanup on a C18 cartridge. A Supelcosil LC-18-DB column is used for LC separation, and UV determination is at 220 nm. The isocratic mobile phase is a mixture of methanol, acetonitrile, and water. Mean recoveries from catfish were 88.7, 96.9, and 91.7%; standard deviations were 6.84, 7.78, and 6.26%; and coefficients of variation were 7.72, 8.03, and 6.82% for SIM, ATZ, and PRO, respectively.
Holland
D C
DC
U.S. Food and Drug Administration, Denver Federal Center, CO 80225-0087, USA.
Munns
R K
RK
Roybal
J E
JE
Hurlbut
J A
JA
Long
A R
AR
D000818:Animals; D001280:Atrazine / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods; D006540:Herbicides / Q000032:analysis*; D007059:Ictaluridae / Q000378:metabolism*; D010573:Pesticide Residues / Q000032:analysis*; D012839:Simazine / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D014227:Triazines / Q000032:analysis
D006540:Herbicides; D010573:Pesticide Residues; D014227:Triazines; D001280:Atrazine; D012839:Simazine; C100043:propazine