PCR analysis of the H ferritin multigene family reveals the existence of two classes of processed pseudogenes.
The human gene coding for the apoferritin H subunit belongs to a complex multigene family constituted by the expressed gene and by an undefined number of pseudogenes. We have used a strategy based on PCR to amplify specifically the H pseudogenes from a sample of human genomic DNA. With this approach, three new H pseudogenes have been cloned and characterized by DNA sequence analysis. In addition, we have identified a new type of pseudogene, the size of which (700 bp) is caused by multiple detection events in the putative coding region.
Quaresima
B
B
Dipartimento di Biochimica e Biotecnologie Mediche, Universitá degli Studi di Napoli Federico II, Italia.
Tiano
M T
MT
Porcellini
A
A
D'Agostino
P
P
Faniello
M C
MC
Bevilacqua
M A
MA
Cimino
F
F
Costanzo
F
F
D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005293:Ferritins / Q000235:genetics*; D017353:Gene Deletion; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011544:Pseudogenes
D018076:DNA, Complementary; D005293:Ferritins