title: The first isolation of Borrelia burgdorferi from Ixodes ricinus (Acari, Ixodidae) ticks in Poland.
abstract: Among ticks Ixodes ricinus collected in April 1994 in the forest near Fraknowo (Olsztyn province, N. C. Poland), 17 ticks were evaluated for Borrelia infection by cultivating in the BSK-H medium (Sigma). The ticks were examined in five pools--four of 3 females and one pool of 5 nymphs. Spirochaetes were cultured successfully only from one pool of females and identified as Borrelia burgdorferi with monoclonal antibodies: H 9724, H 5332, H 3TS and 11 G 1 by indirect immunofluorescence assay (IFA). This is the first reported isolate of B. burgdorferi from Ixodes ricinus in Poland.
authors.affiliation: Institute of Maritime and Tropical Medicine, Gdynia, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Dabrowski
authors.forename: A
authors.initials: A
authors.lastname: Schönberg
authors.forename: Z
authors.initials: Z
authors.lastname: Wegner
authors.forename: J
authors.initials: J
authors.lastname: Stańczak
authors.forename: W
authors.initials: W
authors.lastname: Kruminis-Lozowska
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001096:Arachnid Vectors / Q000382:microbiology*; D015748:Borrelia burgdorferi Group / Q000302:isolation & purification*; D004197:Disease Reservoirs; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D018884:Ixodes / Q000382:microbiology*; D051379:Mice; D009758:Nymph; D011044:Poland; D011817:Rabbits
chemical_list: D000911:Antibodies, Monoclonal