An embryonic alternative transcription initiation site and the 5'-upstream structure of mouse cardiac troponin T gene.
By specific antibody screening of a lambda ZAPII expression library, we identified a class of cDNAs derived from mouse cardiac troponin T (TnT) mRNA with long 5'-ends containing the -30 TATA sequence of the promoter. These long mRNAs indicated the presence of a novel upstream transcription initiation site in the cardiac TnT gene. Primer extension mapping of the mouse cardiac TnT mRNA confirmed an alternative transcription initiation site 55-base pairs upstream of the major initiation site. The upstream initiation site was found significantly more active in the embryonic/neonatal hearts. Genomic cloning and sequencing further revealed an 1.5-kb 5'-upstream sequence of the mouse cardiac TnT gene and relationships between the promoter regulatory elements and the alternative transcription initiation sites.
Jin
J P
JP
Department of Medical Biochemistry, University of Calgary Faculty of Medicine, Alberta, Canada.
Zhang
J
J
Wang
J
J
D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004622:Embryo, Mammalian; D005091:Exons; D015723:Gene Library; D006321:Heart / Q000196:embryology*; D051379:Mice; D008807:Mice, Inbred BALB C / Q000235:genetics*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis; D016385:TATA Box; D014158:Transcription, Genetic; D014336:Troponin / Q000096:biosynthesis* / Q000235:genetics*; D020107:Troponin T
D017931:DNA Primers; D018076:DNA, Complementary; D015345:Oligonucleotide Probes; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014336:Troponin; D020107:Troponin T