title: Serum hepatitis C virus (HCV) RNA: a reliable tool for evaluating HCV-related liver disease in anti-HCV-positive blood donors with persistently normal alanine aminotransferase values.
abstract: BACKGROUND
  The significance of hepatitis C virus (HCV) antibodies in blood donors with persistently normal alanine aminotransferase (ALT) levels requires evaluation.
  
  
  METHODS
  The serum and the liver were assayed for HCV RNA. Liver histology was analyzed in 14 HCV-positive subjects who had repeatedly normal ALT values over a follow-up period of at least 3 months.
  
  
  RESULTS
  HCV RNA was not detected in liver and serum, and liver histology showed minimal changes in more than one-half of the subjects (8/14), even if third-generation recombinant immunoblot assay was positive; this demonstrated that HCV can be eradicated spontaneously. Moderately histopathological liver lesions were usually found in HCV RNA-positive subjects (6/14), but one subject had active disease that required interferon therapy; this shows that chronic hepatitis may be present in HCV-positive individuals despite repeatedly normal transaminase values. HCV genotypes other than 1b (II) were usually identified, and the presence or absence of serum and liver HCV RNA correlated completely in all 14 patients.
  
  
  CONCLUSIONS
  Serum HCV RNA should be assayed in those HCV-positive donors having repeatedly normal transaminase activity; if it is positive, indicating an ongoing HCV infection, a liver biopsy should be performed to measure the degree of the liver disease and determine the appropriate antiviral therapy.
authors.affiliation: Service d'Hépatologie, Hôpital Necker, INSERM U99, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Nalpas
authors.forename: R
authors.initials: R
authors.lastname: Romeo
authors.forename: S
authors.initials: S
authors.lastname: Pol
authors.forename: V
authors.initials: V
authors.lastname: Thiers
authors.forename: F
authors.initials: F
authors.lastname: Carnot
authors.forename: P
authors.initials: P
authors.lastname: Berthelot
authors.forename: C
authors.initials: C
authors.lastname: Brechot
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000032:analysis; D000914:Antibodies, Viral / Q000097:blood*; D005260:Female; D016174:Hepacivirus / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis* / Q000201:enzymology / Q000276:immunology; D006801:Humans; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis*; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral; D000410:Alanine Transaminase