title: Dynamic assessment of intraspinal neural graft survival using magnetic resonance imaging.
abstract: Although previous work has demonstrated the usefulness of magnetic resonance imaging (MRI) for visualizing intraspinal transplants in vivo, the degree to which MRI can differentiate viable fetal neural tissue from evolving spinal cord pathology has not been investigated. Thus, the present study assessed whether MRI performed at earlier postgrafting intervals (0-20 weeks) could document the survival of fetal neural transplants in the injured cat spinal cord. Twelve adult female cats received a hemisection injury at the L1 level, followed immediately by implantation of either embryonic cat spinal cord or neocortex into the cavity. The spinal cords of three control animals were hemisected but received no transplant. Each animal was subsequently imaged at 4 and 8 weeks postoperative. Selected animals from each group were also studied at additional time points ranging from immediately postoperative to 20 weeks. Multislice T2-weighted and intermediate T1-weighted spin-echo images of the lesion or graft site were obtained. Correlative postmortem histological analyses revealed viable donor tissue in 6 of 12 transplant recipients. Spinal cords from the remaining hosts and the control animals all contained cysts at the surgical site that were devoid of donor neural tissue. The graft sites with viable tissue tended to exhibit a slightly hyperintense signal on both intermediate T1-weighted (T1WI) and T2-weighted images (T2WI) throughout the entire experiment. Control cats and cats with failed transplants also were slightly bright on T1WI, but were very hyperintense on T2WI.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Neuroscience, University of Florida College of Medicine, Gainesville, USA.
authors.forename: E D
authors.initials: ED
authors.lastname: Wirth
authors.forename: D P
authors.initials: DP
authors.lastname: Theele
authors.forename: T H
authors.initials: TH
authors.lastname: Mareci
authors.forename: D K
authors.initials: DK
authors.lastname: Anderson
authors.forename: P J
authors.initials: PJ
authors.lastname: Reier
mesh_terms: D000818:Animals; D016380:Brain Tissue Transplantation; D002415:Cats; D002470:Cell Survival; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging; D009474:Neurons / Q000637:transplantation*; D013116:Spinal Cord / Q000637:transplantation*; D013997:Time Factors