Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water.
A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially inoculated samples without enrichment and within 24 h.
Arias
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CR
Departamento de Microbiología, Universitat de Valencia, Burjassot, Spain.
Garay
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E
Aznar
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R
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005069:Evaluation Studies as Topic; D005399:Fishes / Q000382:microbiology*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity; D014733:Vibrio / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D014871:Water Microbiology
D017931:DNA Primers; D004269:DNA, Bacterial; D012338:RNA, Ribosomal, 23S