Simultaneous determination of itraconazole, hydroxy-itraconazole and amphotericin B in human plasma by HPLC with photodiode array detection.
A method for the fast analysis of itraconazole, hydroxy-itraconazole and amphotericin B is described which has the advantage of speed and specificity. The method is based on methanolic extraction of the compounds from plasma, followed by reverse-phase high-performance liquid chromatography with two detection wavelengths (263 and 405 nm). The extraction step provides > or = 94% recovery and the response of the detection system is linear from 50 to 5000 ng/ml. No other drugs have been found to interfere with the assay.
Lacroix
C
C
Laboratoire de pharmacocinétique, centre hospitalier, Le Havre, France.
Wojciechowski
F
F
Danger
P
P
D000666:Amphotericin B / Q000097:blood* / Q000627:therapeutic use; D000935:Antifungal Agents / Q000097:blood* / Q000627:therapeutic use; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D017964:Itraconazole / Q000031:analogs & derivatives* / Q000097:blood* / Q000627:therapeutic use; D009181:Mycoses / Q000188:drug therapy
D000935:Antifungal Agents; C084794:hydroxyitraconazole; D017964:Itraconazole; D000666:Amphotericin B