Simultaneous profile analysis of plasma amino and organic acids by capillary gas chromatography.
A simultaneous GC analysis of more than 20 amino and nearly 30 non-amino organic acids abundant in plasma is for the first time possible. Isolation of the analytes from the plasma matrix is not necessary, keto acids do not require a preliminary oximation. An instantaneous derivatization of the acids with ethyl chloroformate takes place directly in the medium after deproteinization. Less than 30 min are required to prepare a plasma sample for the GC analysis.
Husek
P
P
Institute of Endocrinology, Praha, Czech Republic.
D000596:Amino Acids / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods*; D007651:Keto Acids / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results
D000596:Amino Acids; D007651:Keto Acids