title: Plasticity of gp63 gene organization in Leishmania (Viannia) braziliensis and Leishmania (Viannia) peruviana.
abstract: The genomic organization of gp63 genes in 4 and 7 isolates of Leishmania braziliensis and L. peruviana, respectively was studied by RFLP analysis with 3 restriction enzymes (Bgl I, Sal I and Apa I). Our results showed a marked polymorphism among isolates. Some characters were specific to L. braziliensis or to L. peruviana, and others specific to the respective biogeographical populations of L. peruviana. The average minimum copy number of gp63 genes was found to be higher in L. braziliensis (71) than in L. peruviana (46), suggesting that deletion of gp63 genes might be partially involved in the size decrease of the chromosome bearing gp63 genes, observed between those 2 species (from 700 to 610 kb). Our results may suggest the existence of at least 2 arrays of heterologous gp63 repeats, varying in relative copy number between L. braziliensis and L. peruviana, and among isolates of the latter species. Rearrangement of the gp63 genes was observed during long-term in vitro maintenance of a reference strain of L. braziliensis. These observations document the existence of a dynamic gp63 gene organization in Leishmania of the braziliensis complex.
authors.affiliation: Instituut voor Tropische Geneeskunde Prince Leopold, Antwerpen, Belgium.
authors.forename: K
authors.initials: K
authors.lastname: Victoir
authors.forename: J C
authors.initials: JC
authors.lastname: Dujardin
authors.forename: S
authors.initials: S
authors.lastname: de Doncker
authors.forename: D C
authors.initials: DC
authors.lastname: Barker
authors.forename: J
authors.initials: J
authors.lastname: Arevalo
authors.forename: R
authors.initials: R
authors.lastname: Hamers
authors.forename: D
authors.initials: D
authors.lastname: Le Ray
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D003360:Cosmids; D005784:Gene Amplification; D015321:Gene Rearrangement; D017125:Genes, Protozoan; D007892:Leishmania braziliensis / Q000235:genetics* / Q000302:isolation & purification; D008666:Metalloendopeptidases / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D013045:Species Specificity
chemical_list: D015800:Protozoan Proteins; D008666:Metalloendopeptidases; C055146:glycoprotein gp63, Leishmania