title: The equine herpesvirus 1 gene 63 RING finger protein partially complements Vmw110, its herpes simplex virus type 1 counterpart.
abstract: All alpha herpesviruses of known DNA sequence have been found to encode a protein with similarities to immediate early protein Vmw110 (ICP0) of herpes simplex virus type 1 (HSV-1). The conserved portion of this family of proteins is a characteristic zinc binding module, known as a RING finger or C3HC4 domain. Examples of RING finger domains occur in many other proteins of diverse evolutionary origin and function. Recently, the solution structure of the equine herpesvirus 1 (EHV-1) RING finger protein, encoded by gene 63, has been solved. To investigate whether this structure could be considered to be a paradigm of herpesvirus RING domains, we have constructed a recombinant HSV-1 which expresses the EHV-1 gene 63 protein (EHVg63) in place of Vmw110. Comparison of the growth properties of the recombinant with those of wild-type and Vmw110-defective viruses indicates that EHVg63 is able to fulfil partially, but not completely, the roles of Vmw110 during virus growth in tissue culture.
authors.affiliation: MRC Virology Unit, Institute of Virology, Glasgow, UK.
authors.forename: R
authors.initials: R
authors.lastname: Everett
authors.forename: A
authors.initials: A
authors.lastname: Orr
authors.forename: M
authors.initials: M
authors.lastname: Elliott
mesh_terms: D000818:Animals; D002460:Cell Line; D005814:Genes, Viral; D005816:Genetic Complementation Test; D004861:Herpesvirus 1, Equid / Q000235:genetics*; D018259:Herpesvirus 1, Human / Q000235:genetics*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics*; D044767:Ubiquitin-Protein Ligases; D014764:Viral Proteins / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D017874:Immediate-Early Proteins; D014764:Viral Proteins; C095533:gene 63 protein, Equine herpesvirus 1; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1