title: Retinoblastoma gene mutation in primary human renal cell carcinoma.
abstract: We searched for possible mutations in the E2F-binding region of retinoblastoma gene in primary human renal cell carcinomas, using polymerase chain reaction and single-strand conformational polymorphism analysis of RNA. Retinoblastoma gene mutation was detected in 1 of 21 cases (5%). DNA sequencing of the polymerase chain reaction product verified that this case had a 6-base deletion at the beginning of exon 8. Our findings suggest that mutation of the retinoblastoma gene is involved in only a subgroup of sporadic human renal cell carcinomas.
authors.affiliation: Department of Urology, Yokohama City University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shuin
authors.forename: S
authors.initials: S
authors.lastname: Torigoe
authors.forename: Y
authors.initials: Y
authors.lastname: Kubota
authors.forename: T
authors.initials: T
authors.lastname: Kishida
authors.forename: M
authors.initials: M
authors.lastname: Hosaka
authors.forename: T
authors.initials: T
authors.lastname: Horikoshi
authors.forename: M
authors.initials: M
authors.lastname: Yao
authors.forename: K
authors.initials: K
authors.lastname: Kondo
authors.forename: N
authors.initials: N
authors.lastname: Sakai
authors.forename: K
authors.initials: K
authors.lastname: Danenberg
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D005091:Exons; D016161:Genes, Retinoblastoma / Q000235:genetics*; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational