Effects of Phytolacca acinosa polysaccharides I combined with interleukin-2 on the cytotoxicity of murine splenocytes against tumor cells.
Phytolacca acinosa polysaccharides I (PAP-I), a kind of purified polysaccharides, isolated from Phytolacca acinosa Roxb was found to significantly augment the cytotoxicity of murine splenocytes and interleukin-2 (IL-2) activated splenocytes against P815 tumor cells in vitro. The optimal concentration of PAP-I was 1 microgram.ml-1 and the peak level of the cytotoxicity against P815 tumor cells was reached on d 3-5. The supernatants collected from splenocytes cultured with PAP-I alone or in combination with IL-2 showed no effect on the cytotoxicity against P815 tumor cells. Splenocytes from mice injected ip with PAP-I, 5, 10 and 50 mg.kg-1, thrice a week produced more cytotoxicity against P815 and L929 tumor cells compared with the control group. PAP-I ip was shown to significantly increase IL-2 activated killer cell activity (LAK) against P815 tumor cells. The higher the dosage of PAP-I, the more potent the LAK activity was observed. These results confirmed that PAP-I can augment the cytotoxicity of murine splenocyte against tumor cells and LAK activity and warranted further evaluation of its clinical usefulness.
Wang
H B
HB
Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai.
Chen
W Z
WZ
Bao
E J
EJ
Zheng
Q Y
QY
Song
H L
HL
Fang
J
J
Xu
Y X
YX
Chen
H S
HS
D000818:Animals; D003602:Cytotoxicity, Immunologic / Q000187:drug effects*; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005260:Female; D007376:Interleukin-2 / Q000494:pharmacology*; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D004365:Drugs, Chinese Herbal; D007376:Interleukin-2; D011134:Polysaccharides