title: NT-3 combined with CNTF promotes survival of neurons in modiolus-spiral ganglion explants.
abstract: Auditory neurons depend upon the integrity of both their peripheral (auditory hair cells) and central (cochlear nucleus) targets for survival. One proposed trophic mechanism is the production of neurotrophin-3 (NT-3) by auditory hair cells. Modiolus-spiral ganglion explants from adult rats that closely mirror cell-cell interactions and in vivo tissue relationships within this ganglion provide a model for testing trophic factors. Brain derived neurotrophic factor (BDNF), NT-3 and ciliary neurotrophic factor (CNTF) were tested for their ability, both individually and in combination, to support neuronal survival. NT-3 was the strongest individual promoter of survival, while CNTF (a cytokine) with NT-3 (a neurotrophin) was the most effective combination for promoting the survival of auditory neurons.
authors.affiliation: Department of Otolaryngology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
authors.forename: H
authors.initials: H
authors.lastname: Staecker
authors.forename: W
authors.initials: W
authors.lastname: Liu
authors.forename: C
authors.initials: C
authors.lastname: Hartnick
authors.forename: P
authors.initials: P
authors.lastname: Lefebvre
authors.forename: B
authors.initials: B
authors.lastname: Malgrange
authors.forename: G
authors.initials: G
authors.lastname: Moonen
authors.forename: T R
authors.initials: TR
authors.lastname: Van de Water
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor; D002470:Cell Survival / Q000187:drug effects; D020934:Ciliary Neurotrophic Factor; D017626:Cochlear Nucleus / Q000166:cytology / Q000187:drug effects*; D006198:Hair Cells, Auditory / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000096:biosynthesis / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects*; D020933:Neurotrophin 3; D051381:Rats; D017208:Rats, Wistar; D013136:Spiral Ganglion / Q000166:cytology / Q000187:drug effects*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D020934:Ciliary Neurotrophic Factor; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D020933:Neurotrophin 3