title: Axenic cultivation of Entamoeba dispar Brumpt 1925, Entamoeba insolita Geiman and Wichterman 1937 and Entamoeba ranarum Grassi 1879.
abstract: Three species of Entamoeba have been grown in axenic culture for the first time. In two cases, novel methods for adapting the organisms to growth without bacteria were employed. While E. ranarum was axenized by the classic technique of Diamond, from a monoxenic culture with Trypanosoma cruzi as the associate, both E. dispar and E. insolita were first grown in axenic culture medium supplemented with lethally irradiated bacteria. From there, E. insolita was axenized directly, but E. dispar initially required the presence of fixed bacteria. After prolonged culture under this technically axenic but unwieldy culture system, E. dispar was eventually adapted to growth in the absence of added bacteria.
authors.affiliation: Laboratory of Parasitic Diseases, NIAID, NIH, Bethesda, Maryland 20892-0425, USA.
authors.forename: C G
authors.initials: CG
authors.lastname: Clark
mesh_terms: D000818:Animals; D001419:Bacteria; D016831:Crithidia fasciculata; D003470:Culture Media; D004747:Entamoeba / Q000254:growth & development*; D004926:Escherichia coli; D010274:Parasitology / Q000379:methods*; D014349:Trypanosoma cruzi
chemical_list: D003470:Culture Media