title: Genotyping of rotaviruses isolated from sewage.
abstract: Rotaviruses from environmental samples have been genotyped by a seminested reverse transcription PCR assay with serotype-specific primers derived from variable regions of gene 9, which produce different characteristic segment sizes for serotypes 1 to 4. The method enabled the detection and identification of type 1, 2, and 3 group A rotaviruses in sewage.
authors.affiliation: Department of Microbiology, University of Barcelona, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Gajardo
authors.forename: N
authors.initials: N
authors.lastname: Bouchriti
authors.forename: R M
authors.initials: RM
authors.lastname: Pintó
authors.forename: A
authors.initials: A
authors.lastname: Bosch
mesh_terms: D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000706:statistics & numerical data; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D012703:Serotyping; D012722:Sewage; D014871:Water Microbiology
chemical_list: D012722:Sewage