A subset of splenic macrophages process and present native antigen to naive antigen-specific CD4+ T-cells from mice transgenic for an alpha beta T-cell receptor.
The progeny of individual macrophage precursors from mouse spleen were examined for their ability to constitutively process and present native pigeon cytochrome c or a peptide fragment of this antigen to naive CD4+ T-cells from mice transgenic for a V alpha 11/V beta 3 TCR that recognizes an epitope in the antigen fragment. The results show that constitutive Ag processing and presentation is a stable characteristic restricted to the progeny of approximately 20% of splenic macrophage precursors. This property does not appear to be randomly acquired, but to reflect the ability of certain macrophages to produce IL-12.
Askew
D
D
Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.
Gatewood
J
J
Olivas
E
E
Havenith
K
K
Walker
W S
WS
D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology* / Q000378:metabolism; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D010856:Columbidae; D003574:Cytochrome c Group / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010446:Peptide Fragments / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics* / Q000276:immunology; D013154:Spleen / Q000166:cytology*; D013234:Stem Cells / Q000276:immunology
D003574:Cytochrome c Group; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D010446:Peptide Fragments; D016693:Receptors, Antigen, T-Cell, alpha-beta