Empirical evaluation of the influence of side chains on the conformational entropy of the polypeptide backbone.
Changes in amino acid side chains have long been recognized to alter the range and distribution of phi, psi angles found in the main chain of polypeptides. Altering the range and distribution of phi, psi angles also alters the conformational entropy of the flexible denatured state and may thus stabilize or destabilize it relative to the comparatively conformationally rigid native state. A database of 12,320 residues from 61 nonhomologous, high resolution crystal structures was examined to determine the phi, psi conformational preferences of each of the 20 amino acids. These observed distributions in the native state of proteins are assumed to also reflect the distributions found in the denatured state. The distributions were used to approximate the energy surface for each residue, allowing the calculation of relative conformational entropies for each residue relative to glycine. In the most extreme case, replacement of glycine by proline, conformational entropy changes will stabilize the native state relative to the denatured state by -0.82 +/- 0.08 kcal/mol at 20 degrees C. Surprisingly, alanine is found to be the most ordered residue other than proline. This unexpected result is a result of the high percentage of alanines found in helical conformations. This either indicates that the observed distributions in the native state do not reflect the distributions in the denatured state, or that alanine is much more likely to adopt a helical conformation in the denatured state than residues with longer side chains.(ABSTRACT TRUNCATED AT 250 WORDS)
Stites
W E
WE
Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville 72701-1201, USA.
Pranata
J
J
D000409:Alanine / Q000031:analogs & derivatives / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry*; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D016208:Databases, Factual; D005998:Glycine / Q000031:analogs & derivatives / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry; D013223:Statistics as Topic; D013816:Thermodynamics
D000596:Amino Acids; D011506:Proteins; C024314:N-acetylalanyl-N-methylamide; C065960:acetylglycyl-N-methylamide; D000409:Alanine; D005998:Glycine