title: Sample matrix effects in micellar electrokinetic capillary electrophoresis.
abstract: Several factors related to sample matrix which can influence peak height in micellar electrokinetic capillary chromatography were studied. The ionic strength of the sample did not affect greatly the peak height. High concentration of surfactants or organic solvents in the sample decreased the peak height. On the other hand, using a surfactant in the sample different from the one in the electrophoresis buffer or the addition of polyethylene glycol to the sample enhanced slightly the peak height. A high surfactant concentration in the buffer increased the migration time as well as the plate number and the peak height. Matrix effects are more profound with large than with small sample injections. In general, the effect of sample matrix in MECC is much less than that observed in capillary zone electrophoresis. It is recommended to prepare the standards in the same matrix as that of the sample or to add the analytes directly to the sample to avoid any bias in the results.
authors.affiliation: Department of Pathology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157, USA.
authors.forename: Z K
authors.initials: ZK
authors.lastname: Shihabi
authors.forename: M E
authors.initials: ME
authors.lastname: Hinsdale
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008823:Micelles; D009994:Osmolar Concentration; D011092:Polyethylene Glycols / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification; D012997:Solvents; D013501:Surface-Active Agents
chemical_list: D008823:Micelles; D011506:Proteins; D012997:Solvents; D013501:Surface-Active Agents; D011092:Polyethylene Glycols