srb: a Bacillus subtilis gene encoding a homologue of the alpha-subunit of the mammalian signal recognition particle receptor.
We cloned a Bacillus subtilis gene (srb) encoding a homologue of the mammalian signal recognition particle receptor alpha-subunit (SR alpha). The gene is 987 bp in length and encodes a 329-amino acid protein. The deduced amino acid sequence of the protein shared 26.6, 36.2 and 49.7% identity with those of mammalian SR alpha, archaebacterial DP alpha and Escherichia coli FtsY, respectively. The protein contains three conserved GTP-binding elements like the other three SRP receptor proteins, though the N-terminal portion of the putative B. subtilis protein was shorter than the others. Secondary structure prediction showed than an amphipathic alpha-helix is positioned in the N-terminal region. A defect in srb inhibited cell growth and protein translocation.
Oguro
A
A
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Kakeshita
H
H
Honda
K
K
Takamatsu
H
H
Nakamura
K
K
Yamane
K
K
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D001692:Biological Transport / Q000235:genetics; D003001:Cloning, Molecular; D017124:Conserved Sequence; D005798:Genes, Bacterial / Q000235:genetics*; D006160:Guanosine Triphosphate / Q000378:metabolism; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D018160:Receptors, Cytoplasmic and Nuclear / Q000737:chemistry / Q000235:genetics*; D018000:Receptors, Peptide / Q000737:chemistry / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D018271:Signal Recognition Particle / Q000378:metabolism
D001426:Bacterial Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D018000:Receptors, Peptide; D018271:Signal Recognition Particle; C036318:signal peptide receptor; C096754:srb protein, Bacillus subtilis; D006160:Guanosine Triphosphate