Lack of correlation between DNA-dependent protein kinase activity and tumor cell radiosensitivity.
Lack of DNA-dependent protein kinase (DNA-PK) activity confers radiosensitivity and defective DNA double-strand break repair. Nine human malignant glioma cell lines were studied to determine whether differences in DNA-PK activity reflect differences in inherent radiosensitivity or are predictive of tumor treatment response. DNA-PK activity was present in all cell extracts, as were the DNA-PK proteins, DNA-PK catalytic subunit, Ku p70, and Ku p80. No correlation was found between the levels of DNA-PK activity and inherent radiosensitivity or in the tumor treatment response. These preliminary results suggest that variation in DNA-PK activity may not be a determinant of clinical response in malignant glioma.
Allalunis-Turner
M J
MJ
Radiobiology, M. J.A-T., G. M. B. Program, Cross Cancer Institute, Edmonton, Alberta, Canada.
Lintott
L G
LG
Barron
G M
GM
Day
R S
RS
Lees-Miller
S P
SP
D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins; D005910:Glioma / Q000201:enzymology / Q000532:radiotherapy*; D006801:Humans; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011836:Radiation Tolerance; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases