A newly identified GPI-anchored arginine-specific ADP-ribosyltransferase activity in chicken spleen.
Arginine-specific ADP-ribosyltransferase activity was detected in chicken spleen membrane fraction and the activity was extracted by phosphatidylinositol-specific phospholipase C but not by 1 M NaCl or 1% Triton X-100. The transferase activity extracted from the spleen membrane was thiol-independent and was not inhibited by 200 mM NaCl. Zymographic analysis of the transferase, under non-reducing conditions, showed two forms of active bands corresponding to a molecular mass of 46 and 42 kDa. Thus, the presence of this novel arginine-specific ADP-ribosyltransferase, anchored to the membrane through glycosylphosphatidylinositol and different from previously cloned chicken transferases, AT1 and AT2, is being given further attention.
Tsuchiya
M
M
Department of Biochemistry, Shimane Medical University, Izumo, Japan.
Osago
H
H
Shimoyama
M
M
D000818:Animals; D001120:Arginine; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology; D002645:Chickens; D017261:Glycosylphosphatidylinositols / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008623:Mercaptoethanol / Q000494:pharmacology; D017830:Octoxynol / Q000494:pharmacology; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000302:isolation & purification / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013154:Spleen / Q000201:enzymology*; D013379:Substrate Specificity
D017261:Glycosylphosphatidylinositols; D012965:Sodium Chloride; D008623:Mercaptoethanol; D017830:Octoxynol; D001120:Arginine; D011065:Poly(ADP-ribose) Polymerases; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase