title: Cyclopentenyl cytosine and neuroblastoma SK-N-BE(2)-C cell line cells.
abstract: We studied the effect of cyclopentenyl cytosine (CPEC) on human neuroblastoma SK-N-BE(2)-C cell line cells. CPEC had an IC50 value of 100 nM for non-synchronised SK-N-BE(2)-C cells. These cells were arrested in G0/G1-phase or early S-phase of the cell cycle upon treatment with CPEC. After treatment of synchronised S-phase cells with 1 microM CPEC, the number of cells present after 3 days was less than 10% of that observed for the untreated cells. S-phase synchronised cells treated with CPEC and deoxycytidine showed an increased viability in comparison with cells treated with CPEC alone. Approximately 15% of the cells treated with CPEC and deoxycytidine traversed through one cell cycle. The amount of CTP declined to undetectable levels within 3 h after addition of 1 microM CPEC. The presence of cytidine prevented, to a large extent, the cytostatic effect of CPEC.
authors.affiliation: University of Amsterdam, Department of Paediatrics, The Netherlands.
authors.forename: R J
authors.initials: RJ
authors.lastname: Slingerland
authors.forename: A H
authors.initials: AH
authors.lastname: Van Gennip
authors.forename: J M
authors.initials: JM
authors.lastname: Bodlaender
authors.forename: P A
authors.initials: PA
authors.lastname: Voûte
authors.forename: A B
authors.initials: AB
authors.lastname: Van Kuilenburg
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003562:Cytidine / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009447:Neuroblastoma / Q000188:drug therapy* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D003562:Cytidine; C045911:cyclopentenyl cytosine