Contribution of alpha interferon (alpha IFN) to HIV-induced immunosuppression.
In the present investigation we have shown that PBLs taken from HIV-positive patients suppressed T-cell proliferation when cultured for at least 12 days. When infected In vitro with HIV-1, these cells become suppressive after 6 days. PBLs collected from seronegative individuals turned out to be non-suppressive even after prolonged culturing. However, when infected in vitro with HIV they were found to be cytostatic also after 6 days. This anti-immune activity, related to HIV infection, is mediated, at least in part, by alpha IFN, since about 50% of this HIV associated activity could be quenched by alpha IFN antibodies. We also showed that this T-cell cytostasis is associated with CD8+ cells. In view of a possible use of suppressive cells for T-cell vaccination, we verified that such cellular functions were abolished by aldehyde treatment.
Lachgar
A
A
Laboratoire de Physiologie Cellulaire, Université Pierre et Marie Curie, Paris, France.
Bizzini
B
B
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology*; D000447:Aldehydes / Q000494:pharmacology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D005260:Female; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007108:Immune Tolerance; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000096:biosynthesis / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000821:virology; D008297:Male; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
D000447:Aldehydes; D007106:Immune Sera; D016898:Interferon-alpha