Cloning and characterization of a cDNA encoding the specific phosphatidylcholine transfer protein from bovine liver.
A 1917-bp cDNA clone encoding phosphatidylcholine transfer protein (PC-TP) was identified by screening a bovine liver library. Northern blot analysis demonstrated a 2-kb mRNA transcript in bovine liver, and Southern blotting was consistent with a single bovine PC-TP gene which was shown to be present in a diverse group of vertebrates, but not in yeast. Database comparisons revealed the nucleotide sequence of the clone to be unique and unrelated to other cytosolic lipid TP.
Cohen
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Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Boston, MA 02115, USA.
Green
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RM
D000595:Amino Acid Sequence; D000727:Androgen-Binding Protein; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D049294:Phospholipid Transfer Proteins
D000727:Androgen-Binding Protein; D002352:Carrier Proteins; D049294:Phospholipid Transfer Proteins