Specific imaging of hormone-dependent mammary carcinoma in nude mice with [131I]-anti-estriol 3-sulfate antibody.
We tried to put the estrogen metabolite to use in tumor imaging. The antibody against estriol 3-sulfate (E3 3-S), which was one of the major metabolites of estrogen in hormone-dependent mammary carcinoma, was prepared and the tissue distribution and imaging of human breast carcinoma with anti-E3 3-S antibody (Ab) were studied in nude mice. In hormone-dependent breast carcinoma, MCF-7,-bearing nude mice, [125I] anti-E3 3-S Ab localized in tumor with the percentage injected dose/g of 9.29 +/- 3.01 (mean +/- SD). This value was significantly high compared with that in hormone-independent breast carcinoma, MDA-MB-231,-bearing nude mice. At 72 h after the administration of [125I]anti-E3 3-S Ab to MCF-7 bearing mice, tumor/blood, tumor/liver and tumor/muscle ratios were 0.49, 5.02 and 6.83, respectively. These ratios were supposed to be enough for imaging. In radioimmunoscintigraphy, a MCF-7 tumor was clearly visualized at 120 or 168 h post-injection of [131I]anti-E3 3-S Ab.
Shimura
N
N
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Japan.
Kojima
S
S
Kubodera
A
A
Tsuruo
T
T
D000818:Animals; D003937:Diagnosis, Differential; D004964:Estriol / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D008325:Mammary Neoplasms, Experimental / Q000175:diagnosis / Q000000981:diagnostic imaging* / Q000503:physiopathology; D051379:Mice; D008819:Mice, Nude; D009376:Neoplasms, Hormone-Dependent / Q000175:diagnosis / Q000000981:diagnostic imaging* / Q000503:physiopathology; D016719:Radioimmunodetection; D014018:Tissue Distribution
D007457:Iodine Radioisotopes; C027974:estriol 3-sulfate; D004964:Estriol