In vitro and in vivo antimicrobial action of fluphenazine.
The antipsychotic drug fluphenazine was obtained in a dry powder form and was screened with respect to 482 strains of bacteria, which included 170 Gram-positive and 326 Gram-negative strains. Nutrient agar plates containing increasing concentrations of fluphenazine (0-200 micrograms/ml) were used for the determination of the minimum inhibitory concentration (MIC) which was demonstrated by inoculating a loopful of an overnight peptone water culture of the organism on nutrient agar plates and determining the MIC against a control. Fluphenazine was detected to possess pronounced action against both Gram-positive and Gram-negative bacteria at 20-100 micrograms/ml. In the in vivo studies it was seen that when fluphenazine was used at a concentration of 1.5 micrograms/g and 3 micrograms/g mouse body weight both the levels offered significant protection to Swiss strain of white mice when challenged with 50 minimum lethal dose (MLD) of a virulent strain of Salmonella typhimurium 74. The in vivo data with fluphenazine were highly significant (p < 0.001) according to the chi-square test.
Dastidar
S G
SG
Dept of Pharmaceutical Technology, Jadavpur University, Calcutta, India.
Chaudhury
A
A
Annadurai
S
S
Roy
S
S
Mookerjee
M
M
Chakrabarty
A N
AN
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001407:Bacillus / Q000187:drug effects; D004755:Enterobacteriaceae / Q000187:drug effects; D005476:Fluphenazine / Q000494:pharmacology*; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D051379:Mice; D008826:Microbial Sensitivity Tests; D012481:Salmonella Infections, Animal / Q000188:drug therapy / Q000401:mortality; D012486:Salmonella typhimurium / Q000187:drug effects; D013210:Staphylococcus / Q000187:drug effects; D014737:Vibrionaceae / Q000187:drug effects
D000900:Anti-Bacterial Agents; D005476:Fluphenazine