A flow cytometric assay reveals a suppression of phagocytosis by rabbit defensin NP-3A in mouse peritoneal macrophages.
Phagocytosis of fluorescein isothiocyanate (FITC)-labeled polystyrene microparticles by peritoneal macrophages from thioglycollate-elicited mice was examined by means of flow cytometry (FCM). This assay revealed that rabbit defensin NP-3A suppressed the phagocytosis in a dose-dependent manner. The present results suggest that NP-3 released from neutrophils is one of the mediators which modulates the activity of macrophages in response to infection.
Ichinose
M
M
Department of Physiology, Shimmane Medical University, Japan.
Sawada
M
M
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology*; D023082:Defensins; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D005260:Female; D005434:Flow Cytometry; D007840:Latex; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D010587:Phagocytosis / Q000187:drug effects*; D011817:Rabbits; D023084:alpha-Defensins
D001798:Blood Proteins; D023082:Defensins; D007840:Latex; D023084:alpha-Defensins; C406566:defensin NP-3a