title: A decreased metabolic clearance of glucose is involved in the hyperglycemic effect of a serum temperature induced factor (TIF).
abstract: We have studied the effects of a hyperglycemic temperature induced factor (TIF) on glucose metabolism, in 3 groups of Wistar rats: 10 rats injected with non-heated serum, 10 rats injected with heated serum and 10 rats injected with semi-purified TIF. Seric levels of insulin and glucagon were not modified in rats injected with heated serum. The injection of heated serum induced hyperglycemia (p < 0.0001), a decrease of lactate (p < 0.001) and pyruvate (p < 0.05) levels, and an increase of acetoacetate level (p < 0.001). The levels of beta hydroxybutyrate and amino acids (alanine and glutamine) were not changed. Glucose turn over rate (12.3 +/- 1.3 g/min/kg) and metabolic clearance of glucose (10.0 +/- 0.8 ml/min/kg) were significantly lower in rats treated with heated serum and purified TIF than in controls (respectively, p < 0.05 and p < 0.001). These data suggested that the hyperglycemic effect of heated serum and isolated TIF could correspond to an impaired metabolic clearance of glucose and to an increased gluconeogenesis.
authors.affiliation: Laboratoire de biochimie Cellulaire et Moléculaire en Nutrition, Inserm U 308, Vandoeuvre les Nancy, France.
authors.forename: C
authors.initials: C
authors.lastname: Masson
authors.forename: R
authors.initials: R
authors.lastname: Bougrine
authors.forename: F
authors.initials: F
authors.lastname: Bois
authors.forename: A
authors.initials: A
authors.lastname: Zaïd
authors.forename: J P
authors.initials: JP
authors.lastname: Nicolas
authors.forename: J L
authors.initials: JL
authors.lastname: Guéant
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis; D001803:Blood Transfusion; D005947:Glucose / Q000493:pharmacokinetics*; D006358:Hot Temperature; D006943:Hyperglycemia / Q000378:metabolism*; D008657:Metabolic Clearance Rate; D051381:Rats; D017208:Rats, Wistar
chemical_list: D005947:Glucose