Determination of furazolidone in porcine tissue using thermospray liquid chromatography-mass spectrometry and a study of the pharmacokinetics and stability of its residues.
A method is presented for the detection of the nitrofuran, furazolidone, in porcine tissue. Following methanol-buffer extraction of the tissue, liquid partitioning, and solid-phase clean-up, samples are analysed by using thermospray LC-MS monitoring the positive ion m/z 243 with filament-assisted ionization. The LOD is 1 microgram kg-1. The assay is used to investigate the depletion of furazolidone from tissue and sample stability post mortem. It is necessary to snap-freeze samples by immersion in liquid nitrogen immediately upon collection in order to improve the stability of residues in tissue.
McCracken
R J
RJ
Department of Agriculture for Northern Ireland, Stormont, Belfast.
Blanchflower
W J
WJ
Rowan
C
C
McCoy
M A
MA
Kennedy
D G
DG
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D001822:Body Burden; D002853:Chromatography, Liquid; D004355:Drug Stability; D005664:Furazolidone / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D013058:Mass Spectrometry; D009132:Muscles / Q000378:metabolism; D013552:Swine / Q000378:metabolism*
D000890:Anti-Infective Agents; D005664:Furazolidone