Inhibitory effect of probucol on nephrotoxicity induced by ferric nitrilotriacetate (Fe-NTA) in rats.
Effects of dietary probucol on renal damage induced by a renal carcinogen, ferric nitrilotriacetate (Fe-NTA), in male Wistar rats were quantitatively investigated with a computer-mediated image analyzer. The kidneys of rats fed a 1% probucol diet were protected from necrosis and lipid peroxidation induced by a single i.p. treatment with Fe-NTA solution at 5 mg Fe/kg body wt and were significantly resistant to a higher dose. For the parameter of lipid peroxidation, Schiff's staining method was utilized to demonstrate the extent of formation of aldehydes, products of lipid peroxidation. Thus following injection of Fe-NTA solution at 10 mg Fe/kg body wt the average areas of tubular necrosis were 85.8% versus 56.9% and the positive areas for Schiff's staining were 15.3% versus 5.6% in the renal cortices of rats fed control of 1% probucol diets respectively. These results indicate that probucol provides protection against Fe-NTA-induced nephrotoxicity through its antioxidant properties. In addition to being a cholesterol-lowering drug, useful for the control of hypercholesterolemia, probucol may therefore be beneficial for prevention and treatment of various pathogenic processes including those leading to carcinogenesis.
Qin
X
X
Department of Pathology, Faculty of Medicine, University of Tokyo, Japan.
Zhang
S
S
Zarkovic
M
M
Yamazaki
Y
Y
Oda
H
H
Nakatsuru
Y
Y
Ishikawa
T
T
Ishikawa
T
T
D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002273:Carcinogens / Q000633:toxicity*; D004032:Diet; D005290:Ferric Compounds / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects / Q000473:pathology*; D007672:Kidney Cortex / Q000187:drug effects / Q000473:pathology; D007680:Kidney Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000633:toxicity; D011341:Probucol / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D016588:Anticarcinogenic Agents; D002273:Carcinogens; D005290:Ferric Compounds; D009571:Nitrilotriacetic Acid; D011341:Probucol; C020326:ferric nitrilotriacetate