Structural elucidation of the O-antigenic polysaccharide from Escherichia coli O44:H18.
The O-antigen polysaccharide of the lipopolysaccharide from the enteroaggregative Escherichia coli O44:H18 has been investigated. Sugar and methylation analysis, 1H- and 13C-NMR spectroscopy revealed that the polysaccharide is composed of pentasaccharide repeating units. The sequence of sugar residues was determined by use of two-dimensional nuclear Overhauser effect spectroscopy and heteronuclear multiple bond correlation experiments. The structure of the repeating unit of the O-antigen from Escherichia coli O44:H18 is as follows. [formula: see text]
Staaf
M
M
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
Widmalm
G
G
Weintraub
A
A
Nataro
J P
JP
D002240:Carbohydrate Sequence; D004926:Escherichia coli / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*
D019081:O Antigens