title: P450aldo in hamster adrenal cortex: immunofluorescent and immuno-gold electron microscopic studies.
abstract: The zonal distribution of aldosterone synthase cytochrome P450 (P450aldo) in the adrenal cortex of male hamsters was investigated by immunofluorescence and electron microscopy, using an anti-P450aldo peptide antibody. On cryostat sections the immunolocalization of P450aldo was confined to the zona glomerulosa cells. On semi-thin plastic sections, P450aldo was shown to be located in mitochondria. Studies in electron microscopy, using the colloidal gold technique, confirmed that P450aldo was located in mitochondria.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Sherbrooke University, QC, Canada.
authors.forename: J G
authors.initials: JG
authors.lastname: LeHoux
authors.forename: D
authors.initials: D
authors.lastname: Martel
authors.forename: J
authors.initials: J
authors.lastname: LeHoux
authors.forename: L
authors.initials: L
authors.lastname: Ducharme
authors.forename: A
authors.initials: A
authors.lastname: Lefebvre
authors.forename: N
authors.initials: N
authors.lastname: Brière
mesh_terms: D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D006224:Cricetinae; D019405:Cytochrome P-450 CYP11B2; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D017979:Gold Colloid; D008297:Male; D008647:Mesocricetus; D016253:Microscopy, Immunoelectron; D013252:Steroid 11-beta-Hydroxylase / Q000032:analysis*
chemical_list: D017979:Gold Colloid; D003577:Cytochrome P-450 Enzyme System; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase