Behavior in vitro of long-term cultured bone marrow or blood cells from chronic myeloid leukemia: adhesion molecules and differentiation antigens as detected by immunocytochemistry.
Long-term cultures (LTC) were established from chronic myeloid leukemic bone marrow or blood. As detected by immunocytochemistry using 25 different monoclonal antibodies, the in vitro cultured cells express a variety of adhesion molecules and differentiation antigens. beta 1 and beta 2 integrins are constantly present on long-term cultured cells. CD4, CD54, CD58, and CD71 markers become highly expressed on the cells after about 10 days in vitro while CD56 is permanently lacking. Only 5 of the 17 patients studied had between 25 and 85 per cent CD33 and CD34 differentiation antigen-positive cells initially in the bone marrow or blood. There was a decrease to less than 10 per cent after six to eight weeks in culture. In later stages of long-term culture, monocytes/macrophages become the dominating cell types. Blast cells are admixed to these cells in varying numbers. In 4 of the 17 cases studied long-term cultures converted to cell lines showing indefinite cell growth. The cells were identified as B cell blasts spontaneously transformed by Epstein Barr virus (EBV). The permanent myeloid and monocytic leukemia cell lines K-562, HL-60, KG-1, RC-2a, CTV-1, THP-1, and U-937 were likewise tested for adhesion molecules and differentiation antigens. A stable marker expression was found, the pattern of which is characteristic of each cell line. CD4 is frequently present on myeloid and monocytic leukemia cell lines (HL-60, RC-2a, THP-1, and U-937). Exceptionally, CD2 and CD34 were shown on KG-1. CD49e, CD49f, CD44, and CD71 are expressed on all cell lines tested.
Pasternak
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Max Delbrück Center for Molecular Medicine, Berlin-Buch, Germany.
Pasternak
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D015703:Antigens, CD / Q000032:analysis; D000943:Antigens, Differentiation / Q000032:analysis; D001752:Blast Crisis; D001853:Bone Marrow / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D018922:HL-60 Cells; D006412:Hematopoietic Stem Cells / Q000473:pathology*; D006801:Humans; D007700:Kinetics; D007938:Leukemia; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood* / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000473:pathology*; D008264:Macrophages / Q000473:pathology; D013997:Time Factors; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules