title: Effect of reperfusion following cerebral ischaemia on the activity of the mitochondrial respiratory chain in the gerbil brain.
abstract: The effect of reperfusion following 30 min of cerebral ischaemia on brain mitochondrial respiratory chain activity has been studied in the gerbil. The state 3 respiration rates with both FAD- and NAD-linked substrates were reduced after ischaemia. After 5 min of reperfusion, state 3 respiration with FAD-linked substrates was restored, but levels of NAD-linked substrates did not return to control values until 30 min of reperfusion. By 120 min of reperfusion state 3 respiration decreased relative to control values with all substrates studied. Measurement of the individual respiratory chain complexes showed that complex I, complex II-III, and complex V activities were reduced after ischaemia. By 5 min of reperfusion complex II-III activity was restored, but the activities of complexes I and V did not return to control values until 30 min of reperfusion. In contrast, complex IV activity was unaffected by ischaemia or 5 and 30 min of reperfusion but was significantly reduced after 120 min of reperfusion, possibly owing to free radical production and lipid peroxidation.
authors.affiliation: Department of Neurochemistry, Institute of Neurology, London, England.
authors.forename: A
authors.initials: A
authors.lastname: Almeida
authors.forename: K L
authors.initials: KL
authors.lastname: Allen
authors.forename: T E
authors.initials: TE
authors.lastname: Bates
authors.forename: J B
authors.initials: JB
authors.lastname: Clark
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002545:Brain Ischemia / Q000378:metabolism* / Q000503:physiopathology; D002560:Cerebrovascular Circulation; D004579:Electron Transport; D005849:Gerbillinae; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption; D015424:Reperfusion; D013997:Time Factors