[Site-specific endonuclease BspKT8 from the thermophilic strain KT8 of Bacillus species].
A site-specific endonuclease capable of recognizing the sequence 5'-AAGCTT-3' was detected and purified to homogeneity from the thermophilic strain of Bacillus species KT8. The endonuclease has a molecular mass of 34 kDa and is found in solution in a monomeric form. The activity of BspKT8 does not depend on ATP and is not stimulated by S-adenosyl-L-methionine. The enzyme displays the highest activity with a broad range of temperatures (37 degrees-48 degrees C). Since DNA cleavage occurs in accordance with the scheme: [formula: see text] the enzyme can be assigned to the class-II of restriction endonucleases and represents an isoschizomer of HindIII.
Chernov
A V
AV
Zheleznaia
L A
LA
Matvienko
N I
NI
D001407:Bacillus / Q000201:enzymology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D006358:Hot Temperature; D013379:Substrate Specificity
D004262:DNA Restriction Enzymes; C024402:endodeoxyribonuclease Bsp
Saĭt-spetsificheskaia éndonukleaza BspKT8 iz termofil'nogo shtamma Bacillus species KT8.