Gel electrophoretic analysis of cellular and secreted proteins from resting and activated rat alveolar macrophages treated with pentamidine isethionate.
Pneumocystic carinii pneumonia, which is a major cause of death among patients suffering from acquired immunodeficiency syndrome, has often been treated successfully with pentamidine isethionate. This study examines pentamidine effects on cellular and secreted proteins from rat alveolar macrophages by two-dimensional gel electrophoresis and computerized image analysis. Over 100 secreted proteins were detected by fluorography. Fluorography showed pentamidine diminished tumor necrosis factor and interleukin-1 release along with other proteins. Effects of combined bacterial lipopolysaccharide and pentamidine were more pronounced on secreted versus cellular proteins in protein amount and pattern difference. Thus pentamidine exhibited a general repressive effect on cellular and secreted protein expression in resting and activated macrophages.
Selkirk
J K
JK
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.
He
C
C
Merrick
B A
BA
D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D016676:Macrophages, Alveolar / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D010419:Pentamidine / Q000494:pharmacology*; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D013050:Spectrometry, Fluorescence
D000935:Antifungal Agents; D011506:Proteins; D010419:Pentamidine