Enzymatic synthesis of mannobioses and mannotrioses by reverse hydrolysis using alpha-mannosidase from Aspergillus niger.
Various manno-oligosaccharides including alpha-D-man-(1 --> 2)-D man and alpha-D-man-(1 --> 2)-alpha-D-man-(1 --> 2)-D-man were formed when a highly concentrated mannose solution was incubated in the presence of alpha-mannosidase from Aspergillus niger. alpha-D-Man-(1 --> 2)-D-man and alpha-D- man-(1 --> 2)-alpha-D-man-(1 --> 2)-D-man were isolated by activated carbon chromatography followed by high performance liquid chromatography using an amino-silica column. In addition to the above oligosaccharides, alpha-D-man-(1 --> 3)-D-man, alpha-D-man-(1 --> 6)-D-man, and alpha-D-man-(1 --> 2)-alpha-D-man-(1 -->6)-D-man were also isolated.
Ajisaka
K
K
Meiji Institute of Health Science, Meiji Milk Products Co., Ltd., Odawara, Japan.
Matsuo
I
I
Isomura
M
M
Fujimoto
H
H
Shirakabe
M
M
Okawa
M
M
D001234:Aspergillus niger / Q000201:enzymology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006031:Glycosylation; D006868:Hydrolysis; D008351:Mannans / Q000138:chemical synthesis*; D008361:Mannosidases / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D014312:Trisaccharides / Q000138:chemical synthesis*; D043323:alpha-Mannosidase
D008351:Mannans; D014312:Trisaccharides; C014647:mannobiose; C023872:mannotriose; D008361:Mannosidases; D043323:alpha-Mannosidase