title: The pyruvate dehydrogenase complex of Saccharomyces cerevisiae is regulated by phosphorylation.
abstract: Mitochondria were isolated from Saccharomyces cerevisiae grown on different carbon sources prior to incubation with [gamma-32P]ATP. A major 46,000-M(r) phosphoprotein, corresponding in M(r) value to the E1 alpha subunit of the yeast pyruvate dehydrogenase complex (PDC), was detected only in mitochondria isolated from cells grown on a fermentable carbon source such as galactose. Immunoprecipitation with subunit-specific antiserum to the E1 component of mammalian or yeast PDC confirmed the identity of this polypeptide. PDC activity in isolated yeast mitochondria could be inactivated in an ATP-dependent fashion and reactivated in the presence of Ca2+ ions.
authors.affiliation: Division of Biochemistry and Molecular Biology, University of Glasgow, Scotland, UK.
authors.forename: A G
authors.initials: AG
authors.lastname: James
authors.forename: R M
authors.initials: RM
authors.lastname: Cook
authors.forename: S M
authors.initials: SM
authors.lastname: West
authors.forename: J G
authors.initials: JG
authors.lastname: Lindsay
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001345:Autoradiography; D002118:Calcium / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D006706:Homeostasis; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D010750:Phosphoproteins / Q000302:isolation & purification / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D011768:Pyruvate Dehydrogenase Complex / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D011768:Pyruvate Dehydrogenase Complex; D000255:Adenosine Triphosphate; D002118:Calcium