Bcl6/Laz3 rearrangements in post-transplant lymphoproliferative disorders.
Post-transplant lymphoproliferative disorders (PTLD) are well-known complications of iatrogenic immune deficiency and are thought to result from the proliferation of B cells infected by the Epstein-Barr virus (EBV). Some large cell lymphomas occurring in the general population carry a rearrangement of the Bcl6/Laz3 zinc-finger-encoding gene. 15 EBV-associated PTLD were tested for the presence of Bcl6/Laz3 rearrangements by Southern blot analysis using two specific probes (F370, F372). One out of 15 cases displayed a rearranged band independent of the germline one. In contrast, 10 lymphoblastoid cell lines and one lymphoblastoid cell line passaged in an SCID mouse carried only germline alleles of Bcl6/Laz3 after Southern blot hybridization. This indicates that genetic abnormalities may also play an important role in the development of some PTLD.
Delecluse
H J
HJ
Department of Pathology, Hôpital E. Herriot, Lyon, France.
Rouault
J P
JP
Jeammot
B
B
Kremmer
E
E
Bastard
C
C
Berger
F
F
D000818:Animals; D015139:Blotting, Southern; D002472:Cell Transformation, Viral / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D015321:Gene Rearrangement; D006566:Herpesviridae Infections / Q000150:complications; D004854:Herpesvirus 4, Human; D006801:Humans; D007165:Immunosuppression Therapy / Q000009:adverse effects*; D008232:Lymphoproliferative Disorders / Q000209:etiology* / Q000235:genetics* / Q000821:virology; D051379:Mice; D016513:Mice, SCID; D009894:Opportunistic Infections / Q000150:complications; D016377:Organ Transplantation / Q000009:adverse effects*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051560:Proto-Oncogene Proteins c-bcl-6; D014157:Transcription Factors / Q000235:genetics*; D014407:Tumor Cells, Cultured; D014412:Tumor Virus Infections / Q000150:complications; D016335:Zinc Fingers
C084103:BCL6 protein, human; C490010:Bcl6 protein, mouse; D004268:DNA-Binding Proteins; D011518:Proto-Oncogene Proteins; D051560:Proto-Oncogene Proteins c-bcl-6; D014157:Transcription Factors