Structural properties and thermal stability of human liver and heart fatty acid binding proteins: a Fourier transform IR spectroscopy study.
The secondary structure and the thermal stability of human liver (L-FABP) and heart (H-FABP) fatty acid-binding proteins were analyzed, in the absence and in the presence of oleic acid, by Fourier transform ir spectroscopy. The study was done in order to gain information on the secondary as well three-dimensional structure of L-FABP and to check the possible H-FABP self-association that has been found to occur in rat and pig H-FABP. Comparison of human L-FABP and H-FABP ir spectra reveals that, in spite of the low sequence homology, the two proteins have similar secondary and probably tertiary structures. The ir data indicates that a larger amount of beta-strands are exposed to the solvent in H-FABP as compared to L-FABP, suggesting minor differences in the three-dimensional structures of these proteins. The binding of oleic acid to L-FABP and H-FABP stabilizes their structures and does not modify their secondary structure. The ir spectra neither confirm nor exclude self-association of human H-FABP.
Tanfani
F
F
Institute of Biochemistry Medical School, University of Ancona, Italy.
Kochan
Z
Z
Swierczynski
J
J
Zydowo
M M
MM
Bertoli
E
E
D000577:Amides / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry*; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000737:chemistry*; D018996:Myelin P2 Protein / Q000737:chemistry*; D009206:Myocardium / Q000737:chemistry*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D019301:Oleic Acid; D009829:Oleic Acids / Q000494:pharmacology; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D025521:Tumor Suppressor Proteins
D000577:Amides; D002352:Carrier Proteins; C493319:FABP1 protein, human; C107608:FABP7 protein, human; C493320:Fabp1 protein, mouse; C493321:Fabp1 protein, rat; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D009829:Oleic Acids; D025521:Tumor Suppressor Proteins; D019301:Oleic Acid