title: Rolling in P-selectin-deficient mice is reduced but not eliminated in the dorsal skin.
abstract: P-selectin-mediated rolling is believed to be important in the recruitment of leukocytes to tissue after ischemia-reperfusion injury. The dorsal skin chamber was used to examine differences in the rolling and stable adhesion of circulating leukocytes in subcutaneous (SC) vessels of P-selectin-deficient and age-matched wild-type mice, both under basal conditions and after ischemia-reperfusion. Rolling in the postcapillary venules in SC tissue of P-selectin-deficient mice was significantly lower than that in wild-type mice under the basal conditions and post-ischemia-reperfusion (P < .05), but was not eliminated by the deletion of the P-selectin gene. No significant difference between P-selectin-deficient and wild-type mice in shear rate or leukocyte-endothelial adhesion was observed up to 24 hours after ischemia-reperfusion. These results show that P-selectin-mediated rolling is not a prerequisite for ischemia-reperfusion-induced leukocyte-endothelial adhesion in the skin.
authors.affiliation: Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, Boston 02114, USA.
authors.forename: S
authors.initials: S
authors.lastname: Yamada
authors.forename: T N
authors.initials: TN
authors.lastname: Mayadas
authors.forename: F
authors.initials: F
authors.lastname: Yuan
authors.forename: D D
authors.initials: DD
authors.lastname: Wagner
authors.forename: R O
authors.initials: RO
authors.lastname: Hynes
authors.forename: R J
authors.initials: RJ
authors.lastname: Melder
authors.forename: R K
authors.initials: RK
authors.lastname: Jain
mesh_terms: D000818:Animals; D001415:Back; D002448:Cell Adhesion; D002465:Cell Movement; D004730:Endothelium, Vascular / Q000166:cytology*; D007511:Ischemia / Q000503:physiopathology*; D007962:Leukocytes / Q000737:chemistry / Q000166:cytology*; D051379:Mice; D019007:P-Selectin / Q000502:physiology*; D015427:Reperfusion Injury / Q000503:physiopathology*; D012867:Skin / Q000098:blood supply*; D012884:Skin Window Technique; D014699:Venules
chemical_list: D019007:P-Selectin