title: PCR analysis of the H ferritin multigene family reveals the existence of two classes of processed pseudogenes.
abstract: The human gene coding for the apoferritin H subunit belongs to a complex multigene family constituted by the expressed gene and by an undefined number of pseudogenes. We have used a strategy based on PCR to amplify specifically the H pseudogenes from a sample of human genomic DNA. With this approach, three new H pseudogenes have been cloned and characterized by DNA sequence analysis. In addition, we have identified a new type of pseudogene, the size of which (700 bp) is caused by multiple detection events in the putative coding region.
authors.affiliation: Dipartimento di Biochimica e Biotecnologie Mediche, Universitá degli Studi di Napoli Federico II, Italia.
authors.forename: B
authors.initials: B
authors.lastname: Quaresima
authors.forename: M T
authors.initials: MT
authors.lastname: Tiano
authors.forename: A
authors.initials: A
authors.lastname: Porcellini
authors.forename: P
authors.initials: P
authors.lastname: D'Agostino
authors.forename: M C
authors.initials: MC
authors.lastname: Faniello
authors.forename: M A
authors.initials: MA
authors.lastname: Bevilacqua
authors.forename: F
authors.initials: F
authors.lastname: Cimino
authors.forename: F
authors.initials: F
authors.lastname: Costanzo
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005293:Ferritins / Q000235:genetics*; D017353:Gene Deletion; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D011544:Pseudogenes
chemical_list: D018076:DNA, Complementary; D005293:Ferritins