Chronic sodium azide treatment decreases membrane-bound protein kinase C activity in the rat hippocampus.
Chronic administration of sodium azide in rats inhibits cytochrome oxidase and produces learning and memory deficits. The present experiment tested the hypothesis that chronic sodium azide treatment might also alter protein kinase C activation. Continuous infusion of sodium azide (400 micrograms/h, sc) in rats for 2 weeks significantly decreases membrane-bound protein kinase C in hippocampus, but not frontal cortex, temporal cortex, or cerebellum. Since protein kinase C activation is correlated with hippocampus-dependent learning, these results suggest a possible biochemical mechanism for azide-induced impairment of learning.
Bennett
M C
MC
National Institute of Aging, National Institutes of Health, Bethesda, Maryland 20892, USA.
Fordyce
D E
DE
Rose
G M
GM
Wehner
J M
JM
D000818:Animals; D001386:Azides / Q000494:pharmacology*; D001931:Brain Mapping; D002531:Cerebellum / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D005625:Frontal Lobe / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects* / Q000502:physiology; D008297:Male; D011939:Mental Recall / Q000187:drug effects*; D009153:Mutagens / Q000494:pharmacology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019810:Sodium Azide; D013570:Synaptic Membranes / Q000187:drug effects*; D013702:Temporal Lobe / Q000187:drug effects
D001386:Azides; D009153:Mutagens; D019810:Sodium Azide; D011493:Protein Kinase C