A method for rapid generation of competitive standard molecules for RT-PCR avoiding the problem of competitor/probe cross-reactions.
Ross
R
R
Department of Dermatology, Johannes Gutenberg-University, Mainz, Germany.
Kleiz
R
R
Reske-Kunz
A B
AB
D000199:Actins / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D016477:Artifacts; D001483:Base Sequence; D001667:Binding, Competitive; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D018076:DNA, Complementary / Q000096:biosynthesis; D015603:Keratinocytes; D009052:Leukemia Virus, Murine / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D019001:Nitric Oxide Synthase / Q000096:biosynthesis / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase; D013698:Templates, Genetic; D013997:Time Factors
D000199:Actins; D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D012194:RNA-Directed DNA Polymerase