title: Adrenal-specific transgene expression and derivation of conditionally immortal rat adrenocortical cell lines.
abstract: Conditional immortalisation of cells is a powerful tool for establishing in vitro models maintaining a differentiated phenotype. We are utilising this approach to derive cell lines that maintain the characteristics of glomerulosa and fasciculata cells of the adrenal cortex. Such cell lines should provide a system in which to study aspects of adrenocortical function that are relevant to hypertension, such as the effects of the renin-angiotensin system on steroidogenesis.
authors.affiliation: Centre for Genome Research, Edinburgh, UK.
authors.forename: S D
authors.initials: SD
authors.lastname: Morley
authors.forename: I
authors.initials: I
authors.lastname: Viard
authors.forename: K L
authors.initials: KL
authors.lastname: Parker
authors.forename: J J
authors.initials: JJ
authors.lastname: Mullins
mesh_terms: D000302:Adrenal Cortex / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D000956:Antigens, Viral; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival / Q000276:immunology; D015870:Gene Expression; D051379:Mice; D008822:Mice, Transgenic; D009928:Organ Specificity / Q000235:genetics; D010641:Phenotype; D051381:Rats; D011916:Rats, Inbred F344; D013539:Simian virus 40 / Q000276:immunology; D013696:Temperature
chemical_list: D000956:Antigens, Viral