title: Multiple gene products are produced from a novel protein kinase transcription region.
abstract: The nonmuscle/smooth muscle myosin light chain kinase (MLCK) and the kinase related protein (KRP) that lacks protein kinase activity are myosin II binding proteins encoded in the vertebrate genome by a true gene within a gene relationship. The genomic organization and expression result in the same amino acid sequence in different molecular contexts from two different sizes of mRNA. We report here the identification and characterization of a third size class of gene products. The protein appears to be a higher molecular weight form of MLCK with additional amino terminal tail sequence which might provide differential subcellular targeting characteristics.
authors.affiliation: Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, Chicago, IL 60611-3008, USA.
authors.forename: D M
authors.initials: DM
authors.lastname: Watterson
authors.forename: M
authors.initials: M
authors.lastname: Collinge
authors.forename: T J
authors.initials: TJ
authors.lastname: Lukas
authors.forename: L J
authors.initials: LJ
authors.lastname: Van Eldik
authors.forename: K G
authors.initials: KG
authors.lastname: Birukov
authors.forename: O V
authors.initials: OV
authors.lastname: Stepanova
authors.forename: V P
authors.initials: VP
authors.lastname: Shirinsky
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D001923:Brain Chemistry; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D005347:Fibroblasts; D015870:Gene Expression; D016547:Kinesins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics; D009219:Myosin-Light-Chain Kinase / Q000737:chemistry* / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D017421:Sequence Analysis; D014158:Transcription, Genetic
chemical_list: D002135:Calcium-Binding Proteins; D009124:Muscle Proteins; D012333:RNA, Messenger; C074152:kinesin-II; D009219:Myosin-Light-Chain Kinase; D016547:Kinesins