title: Cloning of the Rhizopus niveus pyr4 gene and its use for the transformation of Rhizopus delemar.
abstract: We have cloned a pyr4 gene encoding orotidine-5'-monophosphate decarboxylase of the filamentous fungus Rhizopus niveus. The pyr4 gene of R. nivens has an open reading frame composed of 265 amino-acid residues and has two putative introns. We have also isolated a pyr4 mutant of Rhizopus delemar from 5-fluoroorotic acid-resistant mutants and transformed it with the pyr4 gene of R. niveus as a selectable marker. Introduced DNA was integrated into the chromosome in a multiple tandem array. The mitotic stability of the introduced DNA was increased by a repeated sporulation process. The expression of the Escherichia coli beta-glucuronidase gene in R. delemar was successfully obtained under the control of the pgk2 gene promoter of R. niveus by co-transformation with the pyr4 gene.
authors.affiliation: Department of Biotechnology, University of Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Horiuchi
authors.forename: N
authors.initials: N
authors.lastname: Takaya
authors.forename: K
authors.initials: K
authors.lastname: Yanai
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: A
authors.initials: A
authors.lastname: Ohta
authors.forename: M
authors.initials: M
authors.lastname: Takagi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002262:Carboxy-Lyases; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005819:Genetic Markers; D008969:Molecular Sequence Data; D009154:Mutation; D012233:Rhizopus / Q000145:classification / Q000235:genetics*; D014170:Transformation, Genetic
chemical_list: D005656:Fungal Proteins; D005819:Genetic Markers; D002262:Carboxy-Lyases; C096137:pyr4 protein, Rhizopus