title: Entry of Listeria monocytogenes into hepatocytes requires expression of inIB, a surface protein of the internalin multigene family.
abstract: The intracellular bacterium Listeria monocytogenes can invade several types of normally non-phagocytic cells. Entry into cultured epithelial cells requires the expression of inIA, the first gene of an operon, comprising two genes: inIA, which encodes internalin, an 800-amino-acid protein, and inIB, which encodes a 630-amino-acid protein. Several genes homologous to inIA are detected in the genome of L. monocytogenes; InIB is one of them. We have assessed the role of inIB in invasiveness of L. monocytogenes by constructing isogenic chromosomal deletion mutants in the inIAB locus. Our findings indicate that: i) inIB is required for entry of L. monocytogenes into hepatocytes, but not into intestinal epithelial cells; ii) inIB encodes a surface protein; iii) internalin plays a role for entry into some hepatocyte cell lines. These results provide the first insight into the cell tropism displayed by L. monocytogenes.
authors.affiliation: Unite des Intéractions Bactéries-Cellules, Institut Pasteur, Paris, France.
authors.forename: S
authors.initials: S
authors.lastname: Dramsi
authors.forename: I
authors.initials: I
authors.lastname: Biswas
authors.forename: E
authors.initials: E
authors.lastname: Maguin
authors.forename: L
authors.initials: L
authors.lastname: Braun
authors.forename: P
authors.initials: P
authors.lastname: Mastroeni
authors.forename: P
authors.initials: P
authors.lastname: Cossart
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D002872:Chromosome Deletion; D015169:Colony Count, Microbial; D004848:Epithelium / Q000378:metabolism; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D008099:Liver / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D014407:Tumor Cells, Cultured; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; C095768:inlB protein, Listeria monocytogenes