Variability in the growth sustaining capacity of medium batches.
Medium batches, analysed in various spontaneous and mitogen induced proliferation assays, revealed heterogeneity in their growth promoting activity. This can critically affect test results and suggests that culture media can be a source of variability and problems in cell culture work. The manufacturers of media should broaden their quality control of medium batches.
Hügin
A W
AW
Laboratory of Immunopathology, NIAID, National Institute of Health, Bethesday, MD 20892, USA.
Rudikoff
E
E
Morse
H C
HC
D016475:3T3 Cells; D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured / Q000166:cytology*; D003470:Culture Media / Q000592:standards*; D005260:Female; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL
D003470:Culture Media