Mechanism of the activation of proteinase inhibitor synthesis by systemin involves beta-sheet structure, a specific DNA-binding protein domain.
We analyzed a tertiary structure of systemin, the first identified polypeptide plant hormone, using two-dimensional NMR spectroscopy. From these data and molecular dynamics calculations we concluded that the peptide can adopt a Z-like-beta-sheet structure, which has previously been found in many specific DNA-binding proteins. Using DNA-cellulose affinity chromatography, we showed that systemin binds strongly to DNA. We suggest that the specific systemin-DNA interaction, particularly in a promoter region of the proteinase inhibitors, could effect gene expression and thus explain the biological activity of systemin.
Slósarek
G
G
Max-Planck-Institute for Medical Research, Heidelberg, Germany.
Kalbitzer
H R
HR
Mucha
P
P
Rekowski
P
P
Kupryszewski
G
G
Giel-Pietraszuk
M
M
Szymański
M
M
Barciszewski
J
J
D000595:Amino Acid Sequence; D001483:Base Sequence; D002846:Chromatography, Affinity; D003196:Computer Graphics; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007781:Lactoferrin / Q000378:metabolism; D018551:Solanum lycopersicum / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D011480:Protease Inhibitors / Q000378:metabolism*; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D010455:Peptides; D010940:Plant Proteins; D011480:Protease Inhibitors; C073704:systemin; D004247:DNA; D007781:Lactoferrin