Multivessel supercritical fluid extraction of food items in Total Diet Study.
An off-line, large capacity, multivessel supercritical fluid extractor (SFE) was designed and constructed for extraction of large samples. The extractor can simultaneously process 1-6 samples (15-25 g) by using supercritical carbon dioxide (SC-CO2), which is relatively nontoxic and nonflammable, as the solvent extraction medium. Lipid recoveries for the SFE system were comparable to those obtained by blending or Soxhlet extraction procedures. Extractions at 10,000 psi, 80 degrees C, expanded gaseous CO2 flow rates of 4-5 L/min (35 degrees C), and 1-3 h extraction times gave reproducible lipid recoveries for pork sausage (relative standard deviation [RSD], 1.32%), corn chips (RSD, 0.46%), cheddar cheese (RSD, 1.14%), and peanut butter (RSD, 0.44%). In addition, this SFE system gave reproducible recoveries (> 93%) for butter fortified with cis-chlordane and malathion at the 100 ppm and 0.1 ppm levels. Six portions each of cheddar cheese, saltine crackers, sandwich cookies, and ground hamburger also were simultaneously extracted with SC-CO2 and analyzed for incurred pesticide residues. Results obtained with this SFE system were reproducible and comparable with results from organic-solvent extraction procedures currently used in the Total Diet Study; therefore, use and disposal of large quantities of organic solvents can be eliminated.
Hopper
M L
ML
U.S. Food and Drug Administration, Total Diet Research Center, Lenexa, KS 66285-5905, USA.
King
J W
JW
Johnson
J H
JH
Serino
A A
AA
Butler
R J
RJ
D002245:Carbon Dioxide; D002845:Chromatography; D004032:Diet; D005223:Fats / Q000032:analysis; D005504:Food Analysis / Q000295:instrumentation / Q000379:methods*; D010573:Pesticide Residues / Q000032:analysis
D005223:Fats; D010573:Pesticide Residues; D002245:Carbon Dioxide