The influence of dystrophin on lateral diffusion of proteins in sarcolemma of L-185 and C2 myoblasts and mature striated muscle cells of rats and mice, as measured by FRAP technique.
The expression of dystrophin can be suppressed in cultured skeletal muscle cells (coded L-185 from rat, and C2 from mouse) after a proper genetic manipulation. The influence of presence or absence of dystrophin on the lateral diffusion constant of Con-A-receptors was studied in the cell membrane of such cells (and also of mature skeletal muscle fibres of rat and mouse) by means of the fluorescence recovery after photobleaching (FRAP) technique, applying a novel fluorescent label called Con-A-BODIPY-FI conjugate. It has been established that the normal maturation, of myoblasts into skeletal muscle fibres involves a significant decrease of the mobility of Con-A receptors in the sarcolemma. In the absence of dystrophin, this maturation process cannot take place; the membrane proteins display an increasing mobility during the culture time, which is of lethal effect for these cells.
Zs-Nagy
I
I
Verzár International Laboratory for Experimental Gerontology (VILEG), University Medical School, Debrecen, Hungary.
Zhang
X
X
Kitani
K
K
Nonomura
Y
Y
D000818:Animals; D001896:Boron Compounds; D002460:Cell Line; D002642:Chick Embryo; D003208:Concanavalin A; D004058:Diffusion; D016189:Dystrophin / Q000494:pharmacology*; D005456:Fluorescent Dyes; D008297:Male; D051379:Mice; D009124:Muscle Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D010777:Photochemistry; D051381:Rats; D011916:Rats, Inbred F344; D011952:Receptors, Concanavalin A / Q000378:metabolism*; D012508:Sarcolemma / Q000378:metabolism*
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D016189:Dystrophin; D005456:Fluorescent Dyes; D009124:Muscle Proteins; D011952:Receptors, Concanavalin A; D003208:Concanavalin A