Metabolism of 11-deoxycortisol by cytochrome P450(11 beta): identification of reaction products by 1H-NMR and LC/MS-APCI method.
A hitherto-unidentified steroid produced in the reaction mixture of 11-deoxycortisol with bovine cytochrome P450(11 beta) reconstitution system was purified by repeated HPLCs, and subjected to 1H-NMR analysis. The result suggested that the new metabolite was 19-oxo-11-deoxycortisol. This was further confirmed by a new analytical technique using LC/MS equipped with atmospheric pressure chemical ion (APCI)-analyzer.
Ohta
M
M
Laboratory of Nutrition, Koshien College, Nishinomiya, Japan.
Fujii
S
S
Nonaka
Y
Y
Okamoto
M
M
D001274:Atmospheric Pressure; D003350:Cortodoxone / Q000378:metabolism*; D019405:Cytochrome P-450 CYP11B2; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007477:Ions; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D011522:Protons; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism*
D007477:Ions; D011522:Protons; D003577:Cytochrome P-450 Enzyme System; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase; D003350:Cortodoxone