Anti-CD44 treatment abrogates tissue oedema and leukocyte infiltration in murine arthritis.
A ubiquitous cell adhesion receptor, CD44, preferentially binds hyaluronan, a polysaccharide macromolecule that is present in most extracellular matrices. Hyaluronan molecules have large hydrodynamic volumes that entrap substantial amounts of water and can therefore control tissue hydration (swelling). CD44 is overexpressed by synovial cells and leukocytes, and hyaluronan is overproduced in the rheumatoid synovium and in other inflammatory sites. Nevertheless, the role of the CD44-hyaluronan interaction during inflammation is unclear. Our evidence shows that the CD44 receptor plays a critical role in governing the migration of inflammatory leukocytes into the extravascular compartment of the synovium in murine arthritis. An anti-CD44 antibody induces a rapid loss of CD44 from both leukocytes and synovial cells and displays an inhibitory effect on cell-extracellular matrix interactions in the synovium. As a result, the administration of such an antibody abrogates tissue swelling and leukocyte infiltration, two major components of inflammation.
Mikecz
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Department of Orthopedic Surgery, Rush Medical College at Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612, USA.
Brennan
F R
FR
Kim
J H
JH
Glant
T T
TT
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D001168:Arthritis / Q000139:chemically induced / Q000628:therapy*; D001172:Arthritis, Rheumatoid; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D002645:Chickens; D003094:Collagen / Q000633:toxicity; D004195:Disease Models, Animal; D004285:Dogs; D015536:Down-Regulation / Q000187:drug effects; D004487:Edema / Q000209:etiology / Q000628:therapy*; D000939:Epitopes / Q000276:immunology; D018960:Hyaluronan Receptors / Q000276:immunology / Q000502:physiology*; D006820:Hyaluronic Acid / Q000378:metabolism*; D007116:Immunization, Passive; D007074:Immunoglobulin G / Q000276:immunology / Q000494:pharmacology / Q000627:therapeutic use; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D011509:Proteoglycans / Q000633:toxicity; D051381:Rats; D013583:Synovial Membrane / Q000276:immunology / Q000473:pathology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D018960:Hyaluronan Receptors; D007074:Immunoglobulin G; D010446:Peptide Fragments; D011509:Proteoglycans; D006820:Hyaluronic Acid; D003094:Collagen