title: Migration of dendritic cells in response to formyl peptides, C5a, and a distinct set of chemokines.
abstract: Trafficking to tissues and then to lymph nodes is a crucial aspect of the immunobiology of dendritic cells. The present study was designed to identify molecules able to direct the migration of human blood-derived dendritic cells. fMLP (representative of formyl peptides of bacterial origin), C5a, and the C-C chemokines monocyte chemotactic protein (MCP)-3, MIP-1 alpha/LD78, and RANTES elicited chemotactic migration and a rise of intracellular free calcium in dendritic cells. In contrast, the C-X-C chemokines IL-8 and IP-10 and the C-C chemokines MCP-1 and MCP-2 were inactive as chemoattractants. Thus, dendritic cells respond to classical chemotactic signals and to a set of chemokines distinct from that active on monocytes and neutrophils. Chemoattractants are likely to contribute to localization and trafficking of dendritic cells and provide tools to recruit these cells in the design of immunization strategies.
authors.affiliation: Institute for Pharmacological Research Mario Negri, Milan, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Sozzani
authors.forename: F
authors.initials: F
authors.lastname: Sallusto
authors.forename: W
authors.initials: W
authors.lastname: Luini
authors.forename: D
authors.initials: D
authors.lastname: Zhou
authors.forename: L
authors.initials: L
authors.lastname: Piemonti
authors.forename: P
authors.initials: P
authors.lastname: Allavena
authors.forename: J
authors.initials: J
authors.lastname: Van Damme
authors.forename: S
authors.initials: S
authors.lastname: Valitutti
authors.forename: A
authors.initials: A
authors.lastname: Lanzavecchia
authors.forename: A
authors.initials: A
authors.lastname: Mantovani
mesh_terms: D002118:Calcium / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000494:pharmacology*; D002633:Chemotaxis; D015936:Complement C5a / Q000494:pharmacology*; D016207:Cytokines / Q000494:pharmacology*; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans
chemical_list: D002630:Chemotactic Factors; D016207:Cytokines; D015936:Complement C5a; D002118:Calcium