title: Genetic susceptibility to silver-induced anti-fibrillarin autoantibodies in mice.
abstract: Similar to mercuric chloride, silver nitrate has recently been shown to induce IgG autoantibodies targeting the nucleolar 34-kDa protein fibrillarin i SJL (H-2s) mice. In the present study we show that the autoimmunogenic effect of silver is dependent on intact T-cell function since SJL/N mice homozygous for the nude mutation (athymic), in contrast to the functionally T-cell-intact SJL/N-nu/+ littermates, did not develop anti-nucleolar/anti-fibrillarin autoantibodies (ANoA/AFA). The genetic susceptibility for silver-induced AFA was localized to the H-2A locus using congenic and intra-H-2-recombinant strains. However, background (non-H-2) genetic factors substantially influenced both the response rate and the titer of ANoA/AFA attained. Strains bearing H-2As on the SJL and A backgrounds (SJL, A.SW, A.TH) showed 100% response rate and high ANoA titers (3750 +/- 246, mean reciprocal titer +/- SEM), whereas H-2As mice on the B10 background (B10.S) showed 60% response rate and significantly lower ANoA titers (1170 +/- 305) in the responding mice. Expression of H-2E [B10.S(9R) mice] further reduced the response rate (22%) and the ANoA titer (640 +/- 0). A suppressive effect on the B10 background has previously been observed in mercury treatment, but the effect was stronger in silver-treated mice. Two major differences were noted between silver- and mercury-induced murine autoimmunity. First, silver-treated mice did not show elevated titers of other autoantibody specificities, specifically not of antichromatin and anti-histone antibodies, which develop in mercury-treated SJL mice.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pathology I, Linköping University, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Hultman
authors.forename: K
authors.initials: K
authors.lastname: Ganowiak
authors.forename: S J
authors.initials: SJ
authors.lastname: Turley
authors.forename: K M
authors.initials: KM
authors.lastname: Pollard
mesh_terms: D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology*; D015551:Autoimmunity / Q000187:drug effects* / Q000235:genetics*; D001808:Blood Vessels / Q000276:immunology; D002466:Cell Nucleolus / Q000276:immunology; D002843:Chromatin / Q000276:immunology; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology*; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005920:Glomerular Mesangium / Q000276:immunology; D006657:Histones / Q000276:immunology; D015151:Immunoblotting; D051379:Mice; D008815:Mice, Inbred Strains; D012835:Silver Nitrate / Q000633:toxicity*
chemical_list: D000974:Antibodies, Antinuclear; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; C047458:fibrillarin; D012835:Silver Nitrate