Attenuated Shigella as a DNA delivery vehicle for DNA-mediated immunization.
Direct inoculation of DNA, in the form of purified bacterial plasmids that are unable to replicate in mammalian cells but are able to direct cell synthesis of foreign proteins, is being explored as an approach to vaccine development. Here, a highly attenuated Shigella vector invaded mammalian cells and delivered such plasmids into the cytoplasm of cells, and subsequent production of functional foreign protein was measured. Because this Shigella vector was designed to deliver DNA to colonic mucosa, the method is a potential basis for oral and other mucosal DNA immunization and gene therapy strategies.
Sizemore
D R
DR
Department of Bacterial Diseases, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
Branstrom
A A
AA
Sadoff
J C
JC
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D003593:Cytoplasm; D004247:DNA / Q000235:genetics*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D005822:Genetic Vectors; D006168:Guinea Pigs; D007114:Immunization; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D012763:Shigella flexneri / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D004247:DNA; D001616:beta-Galactosidase