Designing DNA-binding proteins on the surface of filamentous phage.
The strategy of molecular evolution by phage display recently has been applied to the study of interactions between protein and DNA. This technology will imminently enable DNA-binding proteins to be made to measure. In the first instance, this will greatly advance our understanding of protein-DNA interactions, but in the long term, it is expected to yield powerful tools for use in medicine and research.
Choo
Y
Y
Medical Research Council, Cambridge, UK.
Klug
A
A
D000818:Animals; D001435:Bacteriophages; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D004247:DNA