Reversed-phase high-performance liquid chromatography--photodiode-array analysis of alkaloid drugs of forensic interest.
A reversed-phase high-performance liquid chromatography-photodiode-array method for the analysis of 23 drugs of forensic interest is presented. The separation method development was based on mobile-phase optimisation, temperature control and use of three ODS stationary phases. Multiwavelength detection and quantitation was performed at 225, 232, 239, 254, 275 and 289 nm. Absorbance rationing proved to be very helpful for the identification of these drugs. Recognition of the analysed compounds was achieved by means of correlation of retention time and absorbance ratios. The method was directly applied to the analysis of illicit heroin and cocaine samples and to the analysis of pharmaceutical preparations containing codeine.
Theodoridis
G
G
Department of Chemistry, Aristotle University, Thessaloniki, Greece.
Papadoyannis
I
I
Vasilikiotis
G
G
Tsoukali-Papadopoulou
H
H
D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003042:Cocaine / Q000032:analysis; D003061:Codeine / Q000032:analysis; D005554:Forensic Medicine / Q000379:methods*; D003932:Heroin / Q000032:analysis; D006358:Hot Temperature; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013696:Temperature
D000470:Alkaloids; D003932:Heroin; D003042:Cocaine; D003061:Codeine