Loss of the chromosomal region 10q23-25 in prostate cancer.
Loss of the chromosomal region 10q23-25 is a frequent event in the progression of prostate adenocarcinoma. A candidate tumor suppressor gene from this region, Mxi1 at 10q25, has recently been shown to be mutated in a small number of prostate tumors. To more strictly define those regions of 10q loss that are likely to be involved in tumor advancement, we have constructed a detailed deletion map spanning 10q23-25 that incorporates Mxi1. Sixty-two % (23 of 37) of tumors analyzed exhibited some degree of 10q23-25 loss. Our data suggest the presence of a prostate tumor suppressor gene(s) near the 10q23-24 boundary, which was deleted in the overwhelming majority (22 of 23) of tumors showing loss. In contrast, specific loss of Mxi1, as opposed to loss of other 10q23-25 regions or of the entire region, was observed in only 1 of 23 tumors and was accompanied by loss of markers at the 10q23-24 boundary. Furthermore, we failed to detect any mutations in Mxi1 in those tumors showing Mxi1-associated marker loss by either single-strand conformation polymorphism analysis or direct DNA sequencing.
Gray
I C
IC
Imperial Cancer Research Fund, Potters Bar, Herts, United Kingdom.
Phillips
S M
SM
Lee
S J
SJ
Neoptolemos
J P
JP
Weissenbach
J
J
Spurr
N K
NK
D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D002872:Chromosome Deletion; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D004276:DNA, Satellite / Q000032:analysis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005453:Fluorescence; D016147:Genes, Tumor Suppressor; D005819:Genetic Markers; D006579:Heterozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D009367:Neoplasm Staging; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins
D051792:Basic Helix-Loop-Helix Proteins; D004273:DNA, Neoplasm; D004276:DNA, Satellite; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D014157:Transcription Factors; D025521:Tumor Suppressor Proteins; C079440:MXI1 protein, human