Measurement of free magnesium in blood, serum and plasma with an ion-sensitive electrode.
The fraction of total magnesium bound to protein and other substances depends upon the pH. pH-dependency of ionized free magnesium (iMg2+) in serum is expressed by the Siggaard-Andersen equation: iMg2+ (pH) = iMg2+ (7.4) x 10x(7.4 - pH). During preparation of serum or plasma, considerable pH changes occur which have to be corrected on the basis of the above mentioned equation. For pH correction of iMg2+, x < 0.1 has so far been used. However, this is correct only for new Mg(2+)-sensitive electrodes. During the lifetime of Mg2+ electrodes used in the "Microlyte Magnesium" (Kone Instruments, Finland) x increases and x = 0.2 was found to be a suitable approximation for most of the lifetime. By instantaneous iMg2+ measurements in whole blood samples pH changes and the uncertainty of x can be avoided. Dilution of blood by intravenous infusions decreases x nearly proportionally to the decrease of protein concentration in blood. Various methodological influences such as temperature and delay time before centrifugation, storage of serum and venous occlusion were studied. The circadian rhythm of iMg2+ was found to be considerably more pronounced than that of total Mg and was negatively correlated to changes of free fatty acids. To avoid variations of iMg2+ due to circadian changes, blood collection should be carried out between 6 and 10 a.m. The normal range of iMg2+ in blood of 179 healthy subjects was found to be between 0.46 and 0.60 mmol/l and the quotient of free and total Mg between 0.59 and 0.71. The accuracy of "Microlyte Magnesium" (Kone Instruments, Finland) is sufficient in a wide range of iMg2+.
Ising
H
H
Umweltbundesamt, Institut für Wasser-, Boden- und Lufthygiene, Berlin, Germany.
Bertschat
F
F
Günther
T
T
Jeremias
E
E
Jeremias
A
A
D001800:Blood Specimen Collection; D002118:Calcium / Q000097:blood; D002940:Circadian Rhythm / Q000502:physiology; D005230:Fatty Acids, Nonesterified / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017736:Ion-Selective Electrodes; D008274:Magnesium / Q000097:blood*; D010949:Plasma / Q000737:chemistry; D012016:Reference Values; D012044:Regression Analysis; D015203:Reproducibility of Results; D013696:Temperature
D005230:Fatty Acids, Nonesterified; D008274:Magnesium; D002118:Calcium