Peroxisome proliferation and associated effects caused by perfluorooctanoic acid in vitamin A-deficient mice.
Vitamin A-deficient male mice were treated for 10 days with 0.02% perfluorooctanoic acid (PFOA) in their diet. The effects of this highly potent peroxisome proliferator on peroxisomal palmitoyl-CoA oxidation and lauroyl-CoA oxidase activities were reduced by 3.1-7.5 times in comparison to the values obtained with mice receiving a vitamin A-adequate diet. The activity of peroxisomal catalase was virtually unaffected by vitamin A deficiency and treatment with PFOA had no significant effect on this activity in vitamin A-adequate or vitamin A-deficient mice. However, vitamin A deficiency itself caused a more than 800% increase in cytosolic catalase activity. Thus, the percentage increase caused by PFOA on cytosolic catalase activity was reduced 12.6 times in vitamin A-deficient mice compared to the increase in vitamin A-adequate mice, although the total absolute activities were similar. These findings suggest that the peroxisome proliferation caused by this peroxisome proliferator is highly dependent on the vitamin A status of the mouse.
Sohlenius
A K
AK
Department of Biochemistry, Wallenberg Laboratory, Stockholm, Sweden.
Andersson
K
K
Olsson
J
J
DePierre
J W
JW
D042962:Acyl-CoA Oxidase; D000818:Animals; D002210:Caprylates / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D004032:Diet; D005466:Fluorocarbons / Q000494:pharmacology*; D008099:Liver / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008830:Microbodies / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D008930:Mitochondria, Liver / Q000378:metabolism / Q000648:ultrastructure; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D014801:Vitamin A / Q000008:administration & dosage; D014802:Vitamin A Deficiency / Q000473:pathology*
D002210:Caprylates; D005466:Fluorocarbons; D014801:Vitamin A; D010171:Palmitoyl Coenzyme A; C023036:perfluorooctanoic acid; D010088:Oxidoreductases; D002374:Catalase; D042962:Acyl-CoA Oxidase