Investigation on the biodurability of chemically different stone wool fibres.
The biodurability is one of the essential factors for a carcinogenic potential of mineral fibres. The in vivo solubility of commercial fibre products can be influenced by modifications of the chemical composition. Two types of experimental stone wool samples with new chemical composition were compared to a commercial stone wool sample. Sized fractions of these samples with median lengths of 7.1, 9.3 and 6.7 microns, respectively, and median diameters of 0.76, 1.02 and 0.63 microns, respectively, were intratracheally instilled into female Wistar rats with a single dose of 2 mg in 0.3 ml. 5 animals per group were sacrificed after 2 days, 1, 3, 6, 12 and 18 months. After low-temperature ashing of the lungs about 1,000 fibres per group and sacrifice date were analysed in SEM for length and diameter. The number of fibres in the total lung was calculated. An analysis of fibre number of different length and diameter fractions was used to estimate whether dissolution, breakage or mechanical clearance is responsible for the elimination of fibres from the lung. Results indicate that the breakage of fibres with length above 20 microns and the dissolution of fibres was faster in the experimental stone wool samples compared to the commercial sample.
Bellmann
B
B
Fraunhofer Inst. Toxicol, Aerosol Res. Hannover, Germany.
Muhle
H
H
Kamstrup
O
O
Draeger
U F
UF
D000818:Animals; D002516:Ceramics / Q000009:adverse effects / Q000737:chemistry*; D005260:Female; D007442:Intubation, Intratracheal; D008168:Lung / Q000737:chemistry / Q000378:metabolism*; D019016:Mineral Fibers / Q000009:adverse effects / Q000032:analysis*; D009079:Mucociliary Clearance; D051381:Rats; D017208:Rats, Wistar
D019016:Mineral Fibers