University of Wisconsin solution effects on intimal proliferation in canine autogenous vein grafts.
This study was carried out to investigate if the University of Wisconsin solution (UWs) is suitable for long-term preservation of autogenous vein grafts (AVG) prior to transplantation, compared to autologous whole blood (AWB) and normal saline (NS). Autogenous jugular and femoral veins were harvested from adult mongrel dogs, using a "no-touch" technique. One segment of vein was immediately implanted to serve as a control, while other segments were stored for 24 hr at 4 degrees C in AWB, NS, or UWs. The control and the preserved veins were implanted as reversed interposition grafts in the common carotid or femoral artery positions. After 6 weeks, scanning electron microscopy revealed an intact endothelial cell monolayer in all vein grafts that was stained positively for factor VIII. The degree of intimal thickening, as assessed by light microscopy in the middle position of the grafts, was similar in controls (52.0 +/- 15.8 microns) and in veins stored in UWs (58.5 +/- 16.1 microns), but it was significantly increased (P < 0.05) in veins preserved in NS (198.9 +/- 19.5 microns) and in AWB (312.0 +/- 171.6 microns). Isometric tension studies revealed that maximum contraction and sensitivities (assessed by EC50) to norepinephrine were significantly reduced (P < 0.05) in AVG stored in AWB (0.05 +/- 0.02 g/mm2 and 5.5 +/- 2.8 microM), but not in UWs (0.16 +/- 0.03 g/mm2 0.92 +/- 0.34 microM) and NS (0.09 +/- 0.03 g/mm2 and 3.0 +/- 1.1 microM), compared with controls (0.17 +/- 0.03 g/mm2 and 0.99 +/- 0.38 microM). Acetylcholine (Ach)-induced maximum relaxations were similar in all of the veins.(ABSTRACT TRUNCATED AT 250 WORDS)
Cavallari
N
N
Creighton Vascular Center, Creighton University School of Medicine, Omaha, Nebraska 68131, USA.
Abebe
W
W
Hunter
W J
WJ
Agrawal
D K
DK
Sapienza
P
P
Mingoli
A
A
Cavallaro
A
A
Edwards
J D
JD
D000109:Acetylcholine / Q000494:pharmacology; D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D002455:Cell Division; D004285:Dogs; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D005978:Glutathione; D007328:Insulin; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D019188:Organ Preservation Solutions; D011887:Raffinose; D014021:Tissue Preservation / Q000379:methods*; D014182:Transplantation, Autologous; D017539:Tunica Intima / Q000166:cytology*; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology; D014680:Veins / Q000166:cytology / Q000637:transplantation*
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D000109:Acetylcholine; D009638:Norepinephrine