Dose-dependent gingival overgrowth induced by cyclosporin in rats.
This study evaluated the effect of dosage on severity of cyclosporin-A (CSA) induced gingival overgrowth. Eighty (80) male Sprague-Dawley rats were randomly distributed into 4 groups. Rats in each group daily received CSA in mineral oil by gastric feeding at dosages of 0 (control), 3, 10, and 30 mg/kg, respectively, for 6 weeks. Stone models of the mandibular incisal region were obtained biweekly and were used for analysis of the gingival dimensions. Animals were sacrificed at the end of week 6 and tissue sections were processed for histopathologic evaluations. Animals were sacrificed at the end of week 6 and tissue sections were processed for histopathologic evaluation Gingival overgrowth including bucco-lingual and mesio-distal width and vertical height were significantly increased with increasing CSA dosage. Furthermore, the gingival dimensions displayed a positive linear relation to dosage and treatment duration. The histopathologic evaluation revealed a granulomatous tissue wedging the tooth-gingival interface in the 3 mg/kg group. This tissue had reached exuberant size in the 10 and 30 mg/kg groups. In summary, the analysis of gingival dimensions the histopathologic evaluation shows a dose-dependent effect on the severity of CSA-induced gingival overgrowth.
Fu
E
E
Department of Periodontology, School of Dentistry, National Defense Medical Center, Taipei, Taiwan, ROC.
Nieh
S
S
Chang
H L
HL
Wang
S L
SL
D000818:Animals; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D016572:Cyclosporine / Q000008:administration & dosage / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000187:drug effects / Q000473:pathology; D005355:Fibrosis; D005885:Gingival Hyperplasia / Q000139:chemically induced* / Q000473:pathology; D006097:Granulation Tissue / Q000187:drug effects / Q000473:pathology; D006099:Granuloma / Q000139:chemically induced / Q000473:pathology; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000009:adverse effects*; D007180:Incisor; D007441:Intubation, Gastrointestinal / Q000295:instrumentation; D008297:Male; D008334:Mandible; D003765:Models, Dental; D015999:Multivariate Analysis; D010513:Periodontal Ligament / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D007166:Immunosuppressive Agents; D016572:Cyclosporine