Structural characterization of an intramolecular RNA triple helix by NMR spectroscopy.
A chemically synthesized 29-base RNA oligomer, designed to fold to form an intramolecular triple helix at acid pH, has been studied by NMR spectroscopy. The molecule consisted of seven U.A.U or C+.G.C base triples joined by two pyrimidine tetra-loops. The fold was such that the third strand was Hoogsteen base-paired in the major groove of a Watson-Crick paired double helix. The nature and size of the molecule required the use of an assignment strategy using two- and three-dimensional homonuclear methods, complemented by a natural abundance 13C correlation experiment. The assignment of the majority of the exchangeable and non-exchangeable resonances is presented. The data suggest a C3'-endo sugar puckering for all the nucleotides involved in base triples. A preliminary structural model consistent with the NMR data is presented.
Klinck
R
R
Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
Liquier
J
J
Taillandier
E
E
Gouyette
C
C
Huynh-Dinh
T
T
Guittet
E
E
D009682:Magnetic Resonance Spectroscopy; D008961:Models, Structural; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*
D012313:RNA