Induction of apoptotic cell death by pancreatitis-associated ascitic fluid in Madin-Darby canine kidney cells.
We investigated the cytotoxicity on Madin-Darby canine kidney (MDCK) cells of pancreatitis-associated ascitic fluid (PAAF) collected from rats with experimental necrotizing pancreatitis. PAAF reduced viability of MDCK cells in a time- and dose-dependent manner. We detected DNA fragmentation on the PAAF-treated MDCK cells, indicating that the cytocidal action of PAAF is via apoptosis. From the results obtained, we conclude that PAAF contains factor(s) inducing apoptosis on MDCK cells, and we assume that apoptotic cell death is involved in the mechanism of organ failure in acute pancreatitis.
Nishikawa
J
J
First Department of Surgery, Kobe University School of Medicine, Japan.
Takeyama
Y
Y
Ueda
T
T
Hori
Y
Y
Ueno
N
N
Yamamoto
M
M
Saitoh
Y
Y
D000208:Acute Disease; D000818:Animals; D017209:Apoptosis; D001201:Ascites / Q000503:physiopathology*; D002460:Cell Line; D002470:Cell Survival; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification; D004249:DNA Damage; D004285:Dogs; D006801:Humans; D007668:Kidney; D007700:Kinetics; D008297:Male; D010195:Pancreatitis / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D004247:DNA