Delay of copper-catalyzed oxidation of low density lipoprotein by in vitro enrichment with choline or ethanolamine plasmalogens.
Low density lipoprotein (LDL) isolated from human serum of different donors was enriched with plasmalogens and their diacyl analogs in order to investigate a possible effect of these phospholipids on the rate of lipid peroxidation in this lipoprotein. LDL was incubated with either vesicles of choline plasmalogen or phosphatidylcholine in presence of lipoprotein- deficient serum, or with liposomes of ethanolamine plasmalogen or phosphatidylethanolamine together with the non-specific phospholipid transfer protein isolated from beef liver. After re-isolation of LDL by ultracentrifugation, a dose-dependent incorporation of the exogenous phospholipids was obtained. Enrichment of LDL with choline plasmalogen resulted in a delay of the copper-catalyzed oxidation of LDL from five different donors. LDL from two donors was also enriched with diacylglycerophosphocholine which led to a delay of oxidation, but the protective effect was smaller than with choline plasmalogen. Enrichment of LDL from two additional donors with ethanolamine plasmalogen resulted in the strongest protection against oxidation, whereas, diacylglycerophospho-ethanolamine had little effect. The delay of the copper-catalyzed LDL oxidation may be due to a direct antioxidative action of the plasmalogens, which are partially degraded during the lag phase of oxidation, or to an indirect effect caused by alteration of the LDL surface in the presence of an excess of glycerophospholipids.
Jürgens
G
G
Institut für Medizinische Biochemie, Karl-Franzens Universität Graz, Austria.
Fell
A
A
Ledinski
G
G
Chen
Q
Q
Paltauf
F
F
D000975:Antioxidants / Q000378:metabolism; D003300:Copper / Q000378:metabolism*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008081:Liposomes / Q000494:pharmacology; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D010713:Phosphatidylcholines / Q000378:metabolism / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000378:metabolism / Q000494:pharmacology; D010955:Plasmalogens / Q000494:pharmacology*
D000975:Antioxidants; D008077:Lipoproteins, LDL; D008081:Liposomes; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010955:Plasmalogens; D003300:Copper