title: Nonradioactive colony hybridization assay for detection and enumeration of enterotoxigenic Clostridium perfringens in raw beef.
abstract: A DNA probe endolabeled with digoxigenin by PCR was developed to detect and enumerate enterotoxigenic Clostridium perfringens in raw beef. After 2 h of hybridization, membranes were developed by using an anti-digoxigenin-alkaline phosphatase conjugated antibody. The resulting chromogenic reaction allowed us to detect and enumerate < or = 10 CFU of C. perfringens per g.
authors.affiliation: Eastern Regional Research Center, U.S. Department of Agriculture, Philadelphia, Pennsylvania 19118, USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Baez
authors.forename: V K
authors.initials: VK
authors.lastname: Juneja
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003016:Clostridium perfringens / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D015169:Colony Count, Microbial / Q000379:methods*; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial; D008460:Meat / Q000382:microbiology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; D004768:Enterotoxins; C035598:enterotoxin, Clostridium