Characterization of a HTLV-I-infected cell line derived from a patient with adult T-cell leukemia with stable co-expression of CD4 and CD8.
A long-term T-cell line, termed SP+, was developed from a human T-cell leukemia virus type I (HTLV-I)-infected patient with adult T-cell leukemia that is dependent on exogenous IL-2 for growth. The SP+ expresses a full complimentation of HTLV-I-specific viral proteins, and contains replication competent viral particles. Restriction enzyme digestion followed by Southern blot analysis demonstrated the presence of a single integrated proviral copy and limiting dilution analysis confirmed the clonality of the cell line. Interestingly, phenotypically, the SP+ cell line is CD2+, CD3+ and coexpresses CD4 and CD8, yet lacks TCR alpha beta and TCR tau delta expression. Further ontogenetic characterization of the SP+ cell line demonstrated the lack of thymic T-cell precursor markers, including absence of cell surface expression of CD1, intracellular thymic terminal deoxynucleotidyl transferase (TdT) enzyme, as well as message expression for V(D)J recombinase activating gene-1 (RAG-1). Furthermore, the SP+ cell did express the message for the CD3 delta chain. Taken together, these data suggest that the SP+ cell line resulted from HTLV-I infection of a mature CD4+/CDB+ lymphocyte. This cell line can be potentially useful as a model, both for regulation of cellular functions by HTLV-I and for immunologic functions of mature dual CD4/CD8 positive T-cells.
Rowe
T
T
Retrovirus Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Dezzutti
C
C
Guenthner
P C
PC
Lam
L
L
Hodge
T
T
Lairmore
M D
MD
Lal
R B
RB
Folks
T M
TM
D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D015704:CD4 Antigens / Q000378:metabolism*; D016827:CD8 Antigens / Q000378:metabolism*; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D015870:Gene Expression; D006678:HIV / Q000254:growth & development; D018398:Homeodomain Proteins; D015368:Human T-lymphotropic virus 1 / Q000254:growth & development; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D007621:Karyotyping; D015458:Leukemia, T-Cell / Q000382:microbiology / Q000473:pathology*; D008875:Middle Aged; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D013950:Thymus Gland / Q000166:cytology; D014407:Tumor Cells, Cultured
D000956:Antigens, Viral; D015704:CD4 Antigens; D016827:CD8 Antigens; D017931:DNA Primers; D018398:Homeodomain Proteins; D011506:Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C064658:RAG-1 protein