Comparison of bronchodilator responses to adrenomedullin and proadrenomedullin N-terminal 20 peptide.
This study was designed to determine and compare airway responses to synthetic human adrenomedullin(AM) and proadrenomedullin N-terminal 20 peptide (PAMP) in anesthetized guinea pigs in vivo. 10(-7) M AM and PAMP significantly inhibited acetylcholine-and histamine-induced bronchoconstriction. However, this significant bronchodilator effect of PAMP lasted about five minutes, which was much shorter than that of AM. In addition, the bronchodilator effect of AM is approximately 100-fold more potent than PAMP. We demonstrated that PAMP had a potent bronchodilator activity, and induced a rapid and short-lasting bronchodilation. These findings suggest that AM and PAMP may play important roles in airway functions.
Kanazawa
H
H
First Department of Internal Medicine, Osaka City University Medical School, Japan.
Kawaguchi
T
T
Fujii
T
T
Kudoh
S
S
Hirata
K
K
Kurihara
N
N
Takeda
T
T
D000109:Acetylcholine / Q000494:pharmacology; D053607:Adrenomedullin; D000818:Animals; D016084:Bronchoconstriction / Q000187:drug effects; D001993:Bronchodilator Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology*; D011498:Protein Precursors / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D013997:Time Factors
D001993:Bronchodilator Agents; D010455:Peptides; D011498:Protein Precursors; D011506:Proteins; C088633:proadrenomedullin; D053607:Adrenomedullin; D006632:Histamine; D000109:Acetylcholine