Effects on CYP27 mRNA expression in rat kidney and liver by 1 alpha, 25-dihydroxyvitamin D3, a suppressor of renal 25-hydroxyvitamin D3 1 alpha-hydroxylase activity.
The production of 1 alpha,25-dihydroxyvitamin D3 is known to be down regulated by 1 alpha,25-dihydroxyvitamin D3 itself. It was recently shown that liver mitochondrial sterol 27-hydroxylase CYP27, present also in kidney, catalyzes 1 alpha-hydroxylation of 25-hydroxyvitamin D3. Treatment of vitamin D-deficient rats with a single i.v. dose of 1 alpha,25-dihydroxyvitamin D3 resulted in a marked suppression of CYP27 mRNA in kidney. The effects were not as pronounced as for CYP24. Liver CYP27 was not affected to the same extent. The results of the present communication indicate a coordinate regulation of CYP27 mRNA levels and 25-hydroxyvitamin D3 1 alpha-hydroxylase activity by 1 alpha,25-dihydroxyvitamin D3 in rat kidney.
Axén
E
E
Department of Pharmaceutical Biosciences, University of Uppsala, Sweden.
Postlind
H
H
Wikvall
K
K
D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase / Q000037:antagonists & inhibitors*; D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D053493:Cholestanetriol 26-Monooxygenase; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013250:Steroid Hydroxylases / Q000235:genetics*; D014808:Vitamin D Deficiency / Q000201:enzymology
D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D053493:Cholestanetriol 26-Monooxygenase; D015090:25-Hydroxyvitamin D3 1-alpha-Hydroxylase; D002117:Calcitriol