Floral chromosomes of Arabidopsis thaliana for detecting low-copy DNA sequences by fluorescence in situ hybridization.
Cosmid and plasmid clones containing 11 kb, or more, of genomic DNA sequences were mapped with high efficiencies using fluorescence in situ hybridization (FISH) to mitotic metaphase chromosomes prepared from floral tissues of Arabidopsis thaliana. The chromosomal locations were correlated with the map positions determined by RFLP (restriction fragment length polymorphism) analyses. Almost no signals were detected on the chromosomes of root meristematic tissues when FISH was performed with the same clones as probes. This discrepancy in efficiency of detection is possibly caused by the differences in chromatin structure between the root meristematic tissues and the floral tissues.
Murata
M
M
Research Institute for Bioresources, Okayama University, Kurashiki 710, Japan.
Motoyoshi
F
F
D017360:Arabidopsis / Q000166:cytology / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D003360:Cosmids; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D008938:Mitosis; D010957:Plasmids