title: Immunoenzymatic methods applied to the search for bioactive taxoids from Taxus baccata.
abstract: Polyclonal antibodies raised against 2'-succinyltaxol-bovine serum albumin (BSA) conjugate were used for the immunodetection of bioactive taxoids in chromatographic fractions of the stem bark extract of Taxus baccata. In addition to taxol, cephalomannine, and baccatin III, two taxoids were isolated and their structures were elucidated as 4 alpha,7 beta-diacetoxy-2 alpha,9 alpha-dibenzoxy-5 beta,20-epoxy-10 beta, 13 alpha, 15-trihydroxy-11(15-->1)-abeo-tax-11-ene[5] and taxol C [6] using spectroscopic methods.
authors.affiliation: Laboratory of Pharmacognosy and Bromatology, Université Libre de Bruxelles, Belgium.
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.forename: R
authors.initials: R
authors.lastname: Vanhaelen-Fastré
authors.forename: B
authors.initials: B
authors.lastname: Diallo
authors.forename: M
authors.initials: M
authors.lastname: Vanhaelen
authors.forename: M
authors.initials: M
authors.lastname: Jaziri
authors.forename: J
authors.initials: J
authors.lastname: Homes
authors.forename: R
authors.initials: R
authors.lastname: Ottinger
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007124:Immunoenzyme Techniques; D007941:Leukemia P388 / Q000188:drug therapy; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D017239:Paclitaxel / Q000031:analogs & derivatives* / Q000737:chemistry* / Q000494:pharmacology; D010936:Plant Extracts / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D017239:Paclitaxel