Comparative characterization of the fermentation pathway of Saccharomyces cerevisiae using biochemical systems theory and metabolic control analysis: steady-state analysis.
In the preceding paper in this issue, we have shown that metabolic control analysis and biochemical systems theory use the same experimental information to describe a metabolic system. In this paper, we analyze the steady-state properties of this pathway by applying both methods. Our results show the correspondence of the steady-state characterizations and illustrate the relationships between the different nomenclatures used. With both approaches, we identify metabolite pools that are strongly influenced by changes in enzyme concentration when cells are immobilized at pH 5.5. In the final paper of this series, which follows, we discuss the need to assess the quality of a model and the potential difficulties that may arise if the steady-state characterization is accepted without testing its quality. We then validate the different models using parameter sensitivity concepts.
Cascante
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Departament de Bioquímica i Fisiología, Facultat de Químiques, Universitat de Barcelona, Spain.
Curto
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Sorribas
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D005285:Fermentation; D007700:Kinetics; D008433:Mathematics; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013598:Systems Theory