The influence of exo-enzyme S and proteases on endogenous Pseudomonas aeruginosa bacteraemia in mice.
The role of Pseudomonas aeruginosa exo-enzymes was evaluated in a murine model of endogenous bacteraemia in which the bacteria invaded the bloodstream after oral dosing. Although an elastase mutant PAO-E64 was as virulent as its parent strain PAO1, an exo-enzyme S-deficient mutant, DG1-ExS5 and alkaline protease mutants PAKS-16, PAKS-17, PAKS-19, were less virulent than their parent strains, DG1 and PAKS-1, respectively (p < 0.01). Thus exo-enzyme S and alkaline protease, but not elastase, appear to contribute to the pathogenicity of P. aeruginosa in this model.
Hirakata
Y
Y
Department of Microbiology, Toho University School of Medicine, Tokyo, Japan.
Furuya
N
N
Tateda
K
K
Matsumoto
T
T
Yamaguchi
K
K
D000818:Animals; D016470:Bacteremia / Q000382:microbiology*; D001426:Bacterial Proteins; D004195:Disease Models, Animal; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008666:Metalloendopeptidases / Q000378:metabolism; D051379:Mice; D009154:Mutation; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism*; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D013047:Specific Pathogen-Free Organisms; D014774:Virulence
D001426:Bacterial Proteins; D011065:Poly(ADP-ribose) Polymerases; D010450:Endopeptidases; D008666:Metalloendopeptidases; C043859:pseudolysin, Pseudomonas aeruginosa