title: Staining etched epoxy resin sections for light microscopy.
abstract: Staining of etched sections for light microscopy is described. Azan staining was successful after treatment with potassium dichromate and the use of concentrated dye solutions. To remove osmium for hematoxylin-eosin staining, removal by reduction with ferrocene was used instead of oxidation. Highly selective differentiation after hematoxylin staining was achieved using p-toluenesulfonic acid-DMSO. To enhance eosin staining, a 2-bromoethylamine link between eosin and the tissue was used. Ferrocene also facilitated counterstaining of nuclei with hematoxylin after the PAS reaction. Periodic acid-methenamine silver staining was carried out without modification.
authors.affiliation: Research Laboratory, Sakura Finetechnical Co., Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Iwadare
authors.forename: T
authors.initials: T
authors.lastname: Arai
mesh_terms: D004853:Epoxy Resins; D006801:Humans; D007668:Kidney / Q000473:pathology; D008853:Microscopy / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004853:Epoxy Resins