title: Gas chromatographic/mass spectrometric confirmation of leucomalachite green in catfish (Ictalurus punctatus) tissue.
abstract: A gas chromatographic/mass spectrometric (GC/MS) method was developed to confirm the presence of leucomalachite green (LMG), a metabolite of the triphenylmethane dye malachite green (MG), in catfish tissue. Residues were isolated according to a previously described liquid chromatographic (LC)/VIS spectrometric analysis of MG and LMG in fish. In our isolation procedure, analytes are extracted from tissue with acetonitrile-buffer, partitioned into CH2Cl2, and applied to neutral alumina and propylsulfonic acid solid-phase extraction cartridges. Before GC/MS analysis, extracts prepared for the LC determinative method are eluted from a cyano solid-phase extraction cartridge, extracted into organic solvent, and concentrated for GC/MS analysis. Selected ion monitoring was performed by using 5 diagnostic ions (m/z 330, 329, 253, 210, and 165) of LMG. The method was validated by confirming LMG in tissue fortified with mixtures of MG and LMG (5 and 10 ng/g each) and in tissue from fish that had been exposed to low levels of MG.
authors.affiliation: U.S. Food and Drug Administration, Denver Federal Center, CO 80225-0087, USA.
authors.forename: S B
authors.initials: SB
authors.lastname: Turnipseed
authors.forename: J E
authors.initials: JE
authors.lastname: Roybal
authors.forename: J A
authors.initials: JA
authors.lastname: Hurlbut
authors.forename: A R
authors.initials: AR
authors.lastname: Long
mesh_terms: D000814:Aniline Compounds / Q000032:analysis*; D000818:Animals; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D007059:Ictaluridae; D012394:Rosaniline Dyes; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods
chemical_list: D000814:Aniline Compounds; D012394:Rosaniline Dyes; C015320:leucomalachite green