title: [RP-HPLC determination of berberine in cortex Phellodendri].
abstract: A reversed-phase high performance liquid chromatographic mothod was developed for the determination of berberine in Cortex Phellodendri using a bonded phase column (Zorbax C8) with diazepam as the internal standard. The column temperature was 25 C. The mobile phase was a mixture of acetonitrile-water (35:65, containing 30mmol/L H3PO4) with a flow rate of lml/min. Berberine was detected by UV absorption at 346nm. The standard curve was linear in the range of 0.14 to 2.0 micrograms. The recovery was 101.7% and RSD 1.83%.
authors.affiliation: Changchun Municipal Institute for Drug Control.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Zhu
authors.forename: D
authors.initials: D
authors.lastname: Wang
authors.forename: K
authors.initials: K
authors.lastname: Wang
mesh_terms: D001599:Berberine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D001599:Berberine