title: Purification from human plasma of a tetrapeptide that potentiates insulin-like growth factor-I activity in chick embryo cartilage.
abstract: Human plasma has been shown to contain a low molecular weight factor that potentiates human IGF-I stimulation of glycosaminoglycan synthesis in chick embryo cartilage. The peptide was purified and characterized by Edman degradation and electrospray mass spectrometry. The primary structure determined was: Trp-Gly-His-Glu. A homologous synthetic peptide similarly promoted matrix biosynthesis in cartilage exposed to IGF-I.
authors.affiliation: Biochemistry Laboratory, Faculty of Medicine, University of Nancy I, Vandoeuvre, France.
authors.forename: J
authors.initials: J
authors.lastname: Straczek
authors.forename: F
authors.initials: F
authors.lastname: Maachi
authors.forename: D
authors.initials: D
authors.lastname: Le Nguyen
authors.forename: M
authors.initials: M
authors.lastname: Becchi
authors.forename: M H
authors.initials: MH
authors.lastname: Heulin
authors.forename: P
authors.initials: P
authors.lastname: Nabet
authors.forename: F
authors.initials: F
authors.lastname: Belleville
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002356:Cartilage / Q000378:metabolism*; D002642:Chick Embryo; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D005109:Extracellular Matrix / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000096:biosynthesis* / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000494:pharmacology
chemical_list: D006025:Glycosaminoglycans; D009842:Oligopeptides; D011994:Recombinant Proteins; C096352:tryptophyl-glycyl-histidyl-glutamic acid; D007334:Insulin-Like Growth Factor I