title: Amperometric thin film biosensors based on glucose dehydrogenase and Toluidine Blue O as catalyst for NADH electrooxidation.
abstract: Amperometric glucose sensors were constructed based on solid graphite electrodes, surface-modified with NAD+ dependent glucose dehydrogenase (GDH), Toluidine Blue O (TBO), and protective ionic polymers. The electrocatalytic oxidation of NADH was evaluated from cyclic voltammetry with TBO dissolved, adsorbed, and electrostatically or covalently bound to polymers. The NADH and glucose sensors constructed were investigated and operated at 0 mV vs. Ag/AgCl using single potential step chronoamperometry. The operational stability of the glucose sensors was limited by leakage of NAD+. A glucose sensitivity much higher than carbon paste electrode was found. A sensitivity as high as 25 microA cm-2 mM-1 was achieved.
authors.affiliation: Moltech Corporation, Stony Brook, NY 11790-3350, USA.
authors.forename: L I
authors.initials: LI
authors.lastname: Boguslavsky
authors.forename: L
authors.initials: L
authors.lastname: Geng
authors.forename: I P
authors.initials: IP
authors.lastname: Kovalev
authors.forename: S K
authors.initials: SK
authors.lastname: Sahni
authors.forename: Z
authors.initials: Z
authors.lastname: Xu
authors.forename: T A
authors.initials: TA
authors.lastname: Skotheim
authors.forename: V
authors.initials: V
authors.lastname: Laurinavicius
authors.forename: B
authors.initials: B
authors.lastname: Persson
authors.forename: L
authors.initials: L
authors.lastname: Gorton
mesh_terms: D015374:Biosensing Techniques; D004800:Enzymes, Immobilized; D005947:Glucose / Q000032:analysis*; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases; D009243:NAD / Q000032:analysis*; D010084:Oxidation-Reduction; D014048:Tolonium Chloride
chemical_list: D004800:Enzymes, Immobilized; D009243:NAD; D014048:Tolonium Chloride; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D005947:Glucose