A simple and rapid staining technique for plastic embedded cartilage and bone.
In this report we describe a simple and rapid staining technique for cartilage and bone embedded in Araldite. Semithin sections of embryonic vertebrae obtained from 15 to 17 day mouse fetuses were stained using an aqueous solution 0.25% with respect to methylene blue, 0.25% with respect to azure A, and 0.5% with respect to Na2 CO3, then counterstained with 1% aqueous pararosaniline chloride (MAP). Results were compared with toluidine blue stained sections. MAP permitted good discrimination of developmental stages of both cells and extracellular matrix within vertebral ossification centers during endochondral ossification. The technique is simple, rapid and applicable to plastic embedded sections, and can be used prior to ultrastructural examination.
Grill
V
V
Department of Human Morphology, University of Trieste, Italy.
Zweyer
M
M
Bareggi
R
R
Martelli
A M
AM
Basa
M
M
Narducci
P
P
D000818:Animals; D001399:Azure Stains; D001842:Bone and Bones / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D002254:Carbonates; D002356:Cartilage / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D004853:Epoxy Resins; D008751:Methylene Blue; D051379:Mice; D010796:Phthalic Anhydrides; D016613:Plastic Embedding; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods*; D014048:Tolonium Chloride; D014052:Toluidines
D001399:Azure Stains; D002254:Carbonates; D004853:Epoxy Resins; D010796:Phthalic Anhydrides; D012394:Rosaniline Dyes; D014052:Toluidines; D014048:Tolonium Chloride; C005409:pararosaniline; C005752:araldite; C005686:sodium carbonate; C025818:Azure A; D008751:Methylene Blue