title: The pathophysiological roles of heterogeneous eosinophils in allergic rhinitis caused by house dust mites.
abstract: We examined the biological characteristics of normodense and hypodense eosinophils prepared from the peripheral blood of the patients with allergic rhinitis caused by house dust mites by measuring leukotriene C4 (LTC4), platelet-activating factor (PAF), and superoxide anions. The normodense (density: 1.100-1.095) and the hypodense (density: 1.080-1.070) eosinophils were prepared by a Percoll density gradient. The normodense eosinophils produced a greater amount of LTC4 (15 ng/10(6) cells) after stimulation by calcium ionophore A23187 than the hypodense eosinophils (1.8 ng/10(6) cells). On the other hand, in the hypodense eosinophils higher amounts of both PAF (5210 pg/10(7) cells/15 min) and superoxide anions (0.33 nmoles/10(7) cells/min) were produced by calcium ionophore A23187 than in the normodense eosinophils, 501 pg/10(7) cells/15 min and 0.18 nmoles/10(7) cells/min, respectively. Considering these results, it is suggested that the two eosinophil subpopulations have distinct biological roles in generating these inflammatory mediators which appear as typical pathological features of allergic rhinitis.
authors.affiliation: Department of Otolaryngology, School of Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Takagi
authors.forename: J
authors.initials: J
authors.lastname: Nishihira
authors.forename: Y
authors.initials: Y
authors.lastname: Koyama
authors.forename: T
authors.initials: T
authors.lastname: Ishibashi
mesh_terms: D000485:Allergens; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002469:Cell Separation; D004391:Dust; D004804:Eosinophils / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D007476:Ionophores / Q000494:pharmacology; D017997:Leukotriene C4 / Q000096:biosynthesis; D008925:Mites; D010972:Platelet Activating Factor / Q000378:metabolism; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology*; D013046:Specific Gravity; D013481:Superoxides / Q000378:metabolism
chemical_list: D000485:Allergens; D004391:Dust; D018836:Inflammation Mediators; D007476:Ionophores; D010972:Platelet Activating Factor; D013481:Superoxides; D017997:Leukotriene C4; D000001:Calcimycin