Participation of the propeptide on procathepsin D activation of human peripheral lymphocytes and neutrophils.
Flow cytometry was used to test the role of the propeptide of procathepsin D in the activation of human peripheral lymphocytes and neutrophils. A selective inhibition of the activation was achieved by antibodies targeted against the propeptide. Synthetic peptide corresponding to the cathepsin D propeptide had a very similar effect as procathepsin D itself. The interaction of procathepsin D with the cells was blocked by the propeptide as monitored in experiments with fluorescently labeled procathepsin D. Our data indicate that procathepsin D activation of human neutrophils and leukocytes described earlier is mediated through the propeptide of the procathepsin D.
Vaclav
V
V
Department of Pathology, University of Louisville, Kentucky 40292, USA.
Jana
V
V
Martin
F
F
D002402:Cathepsin D / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D004792:Enzyme Precursors / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D019041:L-Selectin / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008360:Mannosephosphates / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
D004792:Enzyme Precursors; D016177:Macrophage-1 Antigen; D008360:Mannosephosphates; D019041:L-Selectin; C027693:mannose-6-phosphate; C058345:procathepsin D; D002402:Cathepsin D