Expression pattern of osteogenic protein-1 (bone morphogenetic protein-7) in human and mouse development.
Osteogenic protein-1 (OP-1; BMP-7) is a member of the bone morphogenetic protein subfamily. Because members of the TGF-beta superfamily have a role in tissue development, the distribution of OP-1 expression in developing human embryos (5-8 gestational weeks) and fetuses (8-14 gestational weeks) and mouse (9.5-17.5 gestational days) fetuses was examined. Northern hybridization with specific OP-1 probes revealed two mRNA species of 4 and 2.2 KB. Highest levels of OP-1 mRNA were found in human fetal kidney and heart between 12-14 weeks of gestation. By in situ hybridization, the OP-1 transcripts were found in various tissues, i.e., the ectodermal epithelium of the mouse fore- and hindlimbs, heart, teeth, intestinal epithelium, perichondrium, hypertrophic chondrocytes, and periosteum/osteoblast layer of developing human bones. In kidneys, transcripts were first detected in the epithelium of the branching uretheric buds, whereas at later stages glomeruli were the major site of OP-1 mRNA accumulation. These data suggest that, although OP-1 has been isolated from bone matrix, it may have additional regulatory roles in the morphogenesis and/or function of the kidney, limb bud, tooth, heart, and intestine.
Helder
M N
MN
Bone Research Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland, USA.
Ozkaynak
E
E
Sampath
K T
KT
Luyten
F P
FP
Latin
V
V
Oppermann
H
H
Vukicevic
S
S
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000196:embryology / Q000378:metabolism; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development; D005260:Female; D005333:Fetus; D015870:Gene Expression; D005865:Gestational Age; D006321:Heart / Q000196:embryology; D006801:Humans; D017403:In Situ Hybridization; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D018878:Limb Buds; D008297:Male; D051379:Mice; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D014158:Transcription, Genetic; D016212:Transforming Growth Factor beta / Q000096:biosynthesis
C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D011506:Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta