title: GRO alpha and interleukin-8 in Pneumocystis carinii or bacterial pneumonia and adult respiratory distress syndrome.
abstract: Polymorphonuclear leukocytes (PMN) are the predominant inflammatory cells recruited in acute lung injury. This study compares the concentration of interleukin-8 (IL-8) to those of GRO alpha, both of which are CXC chemokines, in bronchoalveolar lavage fluid (BALF) in three acute pathologic states: bacterial pneumonia (BPN); adult respiratory distress syndrome (ARDS); and Pneumocystis carinii pneumonia (PCP). Levels of both IL-8 and GRO alpha were below 5 pg/ml in 16 nonsmoking volunteers who served as controls. Despite more than twice as many neutrophils in the BALF of the BPN group (n = 12) than in the group with ARDS (n = 13), both groups had similar levels of IL-8, of 569 +/- 120 pg/ml and 507 +/- 96 pg/ml, respectively. The GRO alpha concentrations in the BPN and ARDS patients were respectively 3.3 and 3.4 times those of IL-8, reaching 1,870 +/- 314 pg/ml for the BPN and 1,699 +/- 377 for the ARDS patients. In the PCP group (n = 48, 45 human immunodeficiency virus [HIV]-positive, 3 HIV-negative), GRO alpha levels (897 +/- 172 pg/ml) were sevenfold higher than IL-8 levels (123 +/- 40 pg/ml). In all pathologic states there was a good correlation between GRO alpha and IL-8 (r = 0.53, p = 0.0001). GRO alpha or IL-8 both correlate with the absolute neutrophil number/ml when all groups were studied together (r = 0.52, p = 0.0001). Only in the PCP and ARDS groups did IL-8 correlate with the PMN number.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Medicine, Hôpital Cantonal Universitaire, Geneva, Switzerland.
authors.forename: J
authors.initials: J
authors.lastname: Villard
authors.forename: F
authors.initials: F
authors.lastname: Dayer-Pastore
authors.forename: J
authors.initials: J
authors.lastname: Hamacher
authors.forename: J D
authors.initials: JD
authors.lastname: Aubert
authors.forename: S
authors.initials: S
authors.lastname: Schlegel-Haueter
authors.forename: L P
authors.initials: LP
authors.lastname: Nicod
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000378:metabolism*; D000293:Adolescent; D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D001996:Bronchopneumonia / Q000378:metabolism*; D054360:Chemokine CXCL1; D018925:Chemokines / Q000032:analysis*; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000032:analysis*; D002634:Chemotaxis, Leukocyte; D006133:Growth Substances / Q000032:analysis*; D015497:HIV-1; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8 / Q000032:analysis*; D008297:Male; D009504:Neutrophils / Q000502:physiology; D018410:Pneumonia, Bacterial / Q000378:metabolism*; D011020:Pneumonia, Pneumocystis / Q000378:metabolism*; D012128:Respiratory Distress Syndrome / Q000378:metabolism*; D018709:Statistics, Nonparametric
chemical_list: C475829:CXCL1 protein, human; D054360:Chemokine CXCL1; D018925:Chemokines; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8