Cloning and sequencing of a cDNA encoding rat D-dopachrome tautomerase.
An enzyme which converts D-dopachrome into 5,6-dihydroxyindole has recently been isolated from rat liver. Enzymatic D-dopachrome conversion has been observed in extracts from all tissues examined of several species, including man. We have now cloned and sequenced a 628 bp long cDNA encoding the enzyme provisionally called D-dopachrome tautomerase. The cDNA was isolated by 3' and 5' rapid amplification and cloning of cDNA ends (RACE) from rat liver cells using degenerate oligonucleotide primers, deduced from the N-terminal peptide sequence of D-dopachrome tautomerase. The cDNA contains an open reading frame encoding 118 amino acids. Edman degradation of intact and of trypsin degraded D-dopachrome tautomerase fragments gave information on and corroborated 67% of the deduced protein sequence. A homology search in the EST database found a human cDNA encoding a peptide sharing 66% homology with the rat enzyme. The rat D-dopachrome tautomerase shares 27% homology with the rat macrophage migration inhibitory factor (MIF).
Zhang
M
M
Department of Clinical Genetics, University Hospital, Lund, Sweden.
Aman
P
P
Grubb
A
A
Panagopoulos
I
I
Hindemith
A
A
Rosengren
E
E
Rorsman
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D045563:Indolequinones; D007211:Indoles / Q000378:metabolism; D019746:Intramolecular Oxidoreductases; D007535:Isomerases / Q000737:chemistry* / Q000235:genetics; D008099:Liver / Q000201:enzymology*; D008263:Macrophage Migration-Inhibitory Factors / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011809:Quinones / Q000378:metabolism; D051381:Rats; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D018076:DNA, Complementary; D045563:Indolequinones; D007211:Indoles; D008263:Macrophage Migration-Inhibitory Factors; D011809:Quinones; C001123:dopachrome; D007535:Isomerases; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase