NMR studies of the action of hypochlorous acid on native pig articular cartilage.
The action of sodium hypochlorite on pig articular cartilage was studied by 1H-NMR spectroscopy to model some aspects of degradation processes of cartilage during rheumatoid arthritis. Two effects of NaOCl on cartilage polysaccharides have been observed. Hypochlorous acid causes an enhanced release of oligomeric polysaccharides from cartilage. The second effect concerns the degradation of N-acetyl side chains of carbohydrates to acetate via a chlorinated transient product. Signal intensities for N-acetyl groups (approximately 2.0 ppm) increase during the first 2 h of incubation of cartilage with NaOCl. Then they decrease again. However, acetate (1.90 ppm) as the final product of degradation of N-acetyl side chains increases continuously over the period of incubation with NaOCl. In addition to polysaccharides, effects of NaOCl were only observed in cartilage samples on amino acids like alanine. The alanine resonance disappeared already at NaOCl concentrations where only small effects on cartilage polysaccharides have been observed.
Schiller
J
J
Medical Department, University of Leipzig, Germany.
Arnhold
J
J
Arnold
K
K
D000818:Animals; D001172:Arthritis, Rheumatoid / Q000378:metabolism; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D006997:Hypochlorous Acid / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000378:metabolism*; D013552:Swine
D011134:Polysaccharides; D006997:Hypochlorous Acid