title: Activation of a cell-cycle-regulated histone gene by the oncogenic transcription factor IRF-2.
abstract: The human histone H4 gene FO108 is regulated during the cell cycle with a peak in transcription during early S phase. The cell-cycle element (CCE) required for H4 histone activation is a sequence of 11 base pairs that binds a protein factor in electrophoretic mobility shift assays that has been designated histone nuclear factor M (HiNF-M). Here we report the purification of HiNF-M, and show it to be a protein of relative molecular mass (M(r)) 48K that is identical to interferon (IFN) regulatory factor 2 (IRF-2), a negative transcriptional regulator of the IFN response. Recombinant IRF-2 (as well as the related protein IRF-1 (ref. 5)) binds the CCE specifically and activates transcription of this H4 histone gene. IRF-2 has been shown to have oncogenic potential, and our results demonstrate a link between IRF-2 and a gene that is functionally coupled to DNA replication and cell-cycle progression at the G1/S phase transition.
authors.affiliation: Department of Cell Biology, University of Massachusetts Medical Center, Worcester 01655, USA.
authors.forename: P S
authors.initials: PS
authors.lastname: Vaughan
authors.forename: F
authors.initials: F
authors.lastname: Aziz
authors.forename: A J
authors.initials: AJ
authors.lastname: van Wijnen
authors.forename: S
authors.initials: S
authors.lastname: Wu
authors.forename: H
authors.initials: H
authors.lastname: Harada
authors.forename: T
authors.initials: T
authors.lastname: Taniguchi
authors.forename: K J
authors.initials: KJ
authors.lastname: Soprano
authors.forename: J L
authors.initials: JL
authors.lastname: Stein
authors.forename: G S
authors.initials: GS
authors.lastname: Stein
mesh_terms: D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle / Q000502:physiology*; D002460:Cell Line; D006224:Cricetinae; D004247:DNA; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation; D000882:Haplorhini; D006367:HeLa Cells; D006657:Histones / Q000235:genetics*; D006801:Humans; D050856:Interferon Regulatory Factor-1; D050836:Interferon Regulatory Factor-2; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000378:metabolism; D012097:Repressor Proteins; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D004268:DNA-Binding Proteins; D006657:Histones; C494257:IRF1 protein, human; C494229:IRF2 protein, human; D050856:Interferon Regulatory Factor-1; D050836:Interferon Regulatory Factor-2; D010750:Phosphoproteins; D012097:Repressor Proteins; D014157:Transcription Factors; D004247:DNA