Free radical DNA adduct 8-OH-deoxyguanosine affects activity of Hpa II and Msp I restriction endonucleases.
8-OH-deoxyguanosine can diminish the ability of the restriction endonucleases Hpa II and Msp I to cleave DNA. The exact position of the adduct within the recognition site appears to determine the extent of the effect.
Turk
P W
PW
Department of Medicine, Northwestern University Medical School, Chicago, IL 60611, USA.
Weitzman
S A
SA
D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D003849:Deoxyguanosine / Q000378:metabolism*; D019003:Deoxyribonuclease HpaII / Q000378:metabolism*; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009841:Oligonucleotides; D017382:Reactive Oxygen Species
D005609:Free Radicals; D009841:Oligonucleotides; D017382:Reactive Oxygen Species; D004247:DNA; D004262:DNA Restriction Enzymes; D019003:Deoxyribonuclease HpaII; D003849:Deoxyguanosine