title: Long-term supplementation of canthaxanthin does not inhibit gastric epithelial cell proliferation in Helicobacter mustelae-infected ferrets.
abstract: The effect of canthaxanthin (CX), a nonprovitamin A carotenoid, on gastric epithelial proliferation was studied in ferrets colonized with Helicobacter mustelae, which causes a chronic gastritis and an increased gastric epithelial cell proliferation. Seven spayed female ferrets were dosed by gavage with CX beadlets (50 mg/kg body wt, 5 d/wk) over 2 y, whereas seven control animals were given placebo beadlets. At the end of the 2-y-period, ferrets were killed, and gastric tissues were obtained from the antrum and body regions. A cell-proliferating biomarker, proliferating cell nuclear antigen (PCNA), was assayed by immunohistochemistry. The PCNA positivity was measured by a computer-based image analysis system. Serum concentrations of carotenoids, retinoids and tocopherols were analyzed by HPLC. Serum antioxidant status was measured by the oxygen radical absorbance capacity (ORAC) assay. The PCNA positivity in both antrum and body regions was not significantly different between CX-fed ferrets and controls. Serum CX concentrations were significantly higher in CX-fed ferrets vs. controls (P < 0.001), whereas levels of other carotenoids, retinoids and tocopherols were not significantly different. The ORAC values were not significant different between groups. This study does not suggest inhibitory effects of CX on gastric epithelial cell proliferation in H. mustelae infected ferrets.
authors.affiliation: Gastrointestinal Nutrition Laboratory, Jean Mayer U.S. Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA.
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.forename: J G
authors.initials: JG
authors.lastname: Fox
authors.forename: M C
authors.initials: MC
authors.lastname: Blanco
authors.forename: L
authors.initials: L
authors.lastname: Yan
authors.forename: P
authors.initials: P
authors.lastname: Correa
authors.forename: R M
authors.initials: RM
authors.lastname: Russell
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016644:Canthaxanthin / Q000097:blood / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells; D004848:Epithelium / Q000737:chemistry / Q000382:microbiology; D005260:Female; D005289:Ferrets; D016998:Helicobacter / Q000302:isolation & purification; D016481:Helicobacter Infections / Q000473:pathology*; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D010100:Oxygen / Q000097:blood; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D013270:Stomach / Q000737:chemistry / Q000166:cytology* / Q000382:microbiology*; D013997:Time Factors
chemical_list: D000975:Antioxidants; D018809:Proliferating Cell Nuclear Antigen; D016644:Canthaxanthin; D010100:Oxygen