title: Recombinant A17 Lys human insulin: purification and characterization.
abstract: Recombinant A17 Lys human insulin precursor expressed in yeast cells was isolated from the culture medium. After purification, the precursor was converted into A17 Lys human insulin. A17 Lys human insulin can be crystallized but has much lower receptor-binding and biological activities than porcine or human insulin where A17 is Glu. It is proposed that the A17 Glu residue may be involved in the receptor-binding or the active site of human insulin.
authors.affiliation: State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, China.
authors.forename: B Y
authors.initials: BY
authors.lastname: Zhang
authors.forename: Y S
authors.initials: YS
authors.lastname: Zhang
authors.forename: R R
authors.initials: RR
authors.lastname: Cai
authors.forename: Y M
authors.initials: YM
authors.lastname: Feng
authors.forename: Q B
authors.initials: QB
authors.lastname: He
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003460:Crystallization; D006801:Humans; D007328:Insulin / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D008239:Lysine / Q000502:physiology; D051379:Mice; D010920:Placenta / Q000378:metabolism; D017354:Point Mutation; D011498:Protein Precursors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D011972:Receptor, Insulin / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D013552:Swine; D014357:Trypsin
chemical_list: D000596:Amino Acids; D007328:Insulin; D011498:Protein Precursors; D011994:Recombinant Proteins; D011972:Receptor, Insulin; D014357:Trypsin; D008239:Lysine