Two-dimensional gel electrophoresis and immunoblotting of human serum albumin modified by reaction with penicillins.
A two-dimensional gel electrophoresis and immunoblotting procedure has been developed to assess the level of modification by penicillins in human serum albumin. The procedure can be used in in vitro experiments and in clinical studies with sera from patients treated with penicillins.
Marzocchi
B
B
Dipartimento di Biologia Molecolare, Università di Siena, Italia.
Magi
B
B
Bini
L
L
Cellesi
C
C
Rossolini
A
A
Massidda
O
O
Pallini
V
V
D000667:Ampicillin / Q000494:pharmacology*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D015151:Immunoblotting; D010400:Penicillin G / Q000494:pharmacology*; D010406:Penicillins / Q000494:pharmacology*; D012709:Serum Albumin / Q000032:analysis / Q000187:drug effects*
D010406:Penicillins; D012709:Serum Albumin; D000667:Ampicillin; D010400:Penicillin G