title: Prophylactic use of human endotoxin-core hyperimmune gammaglobulin to prevent endotoxaemia in colostrum-deprived, gnotobiotic lambs challenged orally with Escherichia coli.
abstract: The efficacy of human IgG polyclonal antibody to endotoxin-core in preventing endotoxaemia and subsequent disease was studied in colostrum-deprived gnotobiotic lambs challenged orally at about 5 h old with 10(9) cfu Escherichia coli. Human endotoxin-core hyperimmune gammaglobulin was given intravenously to 5 lambs at 1.9 g IgG/kg bodyweight prior to challenge. Human albumin was given intravenously to 3 control lambs. Bacteraemia was observed in all lambs, but the incidence was lower (P < 0.01) and the onset later (P < 0.05) in gammaglobulin pre-treated lambs. These lambs showed no signs of disease, whereas clinical endotoxaemia, manifesting as watery mouth disease, was diagnosed in 2 of the 3 control lambs which were killed between 18 and 22 h after challenge. Thus, prophylactic treatment of colostrum-deprived lambs with human IgG enriched in endotoxin-core antibodies was effective in reducing the degree of bacteraemia and preventing endotoxaemia, leukopenia and clinical disease following oral challenge with E. coli.
authors.affiliation: Moredun Research Institute, Edinburgh, UK.
authors.forename: J C
authors.initials: JC
authors.lastname: Hodgson
authors.forename: G R
authors.initials: GR
authors.lastname: Barclay
authors.forename: L A
authors.initials: LA
authors.lastname: Hay
authors.forename: G M
authors.initials: GM
authors.lastname: Moon
authors.forename: I R
authors.initials: IR
authors.lastname: Poxton
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000494:pharmacology; D016470:Bacteremia / Q000097:blood / Q000276:immunology / Q000517:prevention & control; D002245:Carbon Dioxide / Q000097:blood; D003126:Colostrum / Q000276:immunology; D004731:Endotoxins / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology; D004927:Escherichia coli Infections / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D005856:Germ-Free Life; D006400:Hematocrit; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D007958:Leukocyte Count; D008297:Male; D012756:Sheep; D014115:Toxemia / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D005719:gamma-Globulins / Q000494:pharmacology*
chemical_list: D000907:Antibodies, Bacterial; D004731:Endotoxins; D007074:Immunoglobulin G; C042058:core endotoxin; D005719:gamma-Globulins; D002245:Carbon Dioxide