title: Influence of hyperglycemia and of hypercapnia on cellular calcium transients during reversible brain ischemia.
abstract: The object of the study was to find out how preischemic hyperglycemia (in normocapnic animals) or excessive hypercapnia (in normoglycemic animals) affect the calcium transient during ischemia, as this can be assessed by measurements of the extracellular calcium concentration ([Ca2+]e). To that extent, normocapnic-normoglycemic control animals were compared with animals with induced hyperglycemia or hypercapnia, all being subjected to 10 min of forebrain ischemia, the [Ca2+]e and d.c. potential being measured with ion-sensitive glass microelectrodes. Hyperglycemia and hypercapnia delayed the loss of ion homeostasis following induction of ischemia. Furthermore, both hyperglycemia and hypercapnia reduced the delay of Ca2+ extrusion upon recirculation. As a result, both hyperglycemia and hypercapnia significantly reduced the ischemic calcium transient, as this was assessed by calculating the duration of maximal calcium load of cells. The results make it less likely that aggravation of brain damage by hyperglycemia or excessive hypercapnia is related to a further derangement of cell calcium homeostasis.
authors.affiliation: Department of Anesthesiology, Helsingborg Hospital.
authors.forename: A
authors.initials: A
authors.lastname: Ekholm
authors.forename: T
authors.initials: T
authors.lastname: Kristián
authors.forename: B K
authors.initials: BK
authors.lastname: Siesjö
mesh_terms: D000138:Acidosis / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry / Q000502:physiology; D015220:Calcium Channels / Q000378:metabolism*; D005260:Female; D006935:Hypercapnia / Q000378:metabolism*; D006943:Hyperglycemia / Q000378:metabolism*; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008839:Microelectrodes; D051381:Rats; D017208:Rats, Wistar
chemical_list: D015220:Calcium Channels