title: Analysis of polychlorinated biphenyls in fatty biological matrixes by on-line supercritical fluid extraction and supercritical fluid cleanup.
abstract: We present a method that combines the extraction of polychlorinated biphenyls (PCBs) and their separation from relatively large quantities of fat in biological matrixes by combined supercritical fluid extraction and separation. Cyano-functionalized silica gel, silica gel, aluminum oxide, Florisil, 3-amino-propyl-functionalized silica gel, and octadecyl-functionalized silica gel were tested for suitability as chromatographic media for separation of PCBs from lipids. Silica gel, 3-aminopropyl-functionalized silica gel, and Florisil adequately separated PCBs from lipids when eluted with supercritical CO2. Florisil allowed both the extraction of PCBs and their separation from lipids in PCB-spiked chicken egg and fish. Two grams of sample containing PCBs at 0.125 microgram/g was sufficient for subsequent separations and the low-level analysis required for the more toxic PCB components.
authors.affiliation: Mississippi State University, Mississippi State Chemical Laboratory 39762, USA.
authors.forename: E G
authors.initials: EG
authors.lastname: Alley
authors.forename: G
authors.initials: G
authors.lastname: Lu
mesh_terms: D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002645:Chickens; D004531:Eggs / Q000032:analysis; D005223:Fats / Q000032:analysis*; D005399:Fishes; D008055:Lipids / Q000032:analysis; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000302:isolation & purification; D012997:Solvents
chemical_list: D005223:Fats; D008055:Lipids; D012997:Solvents; D011078:Polychlorinated Biphenyls