Plant members of a family of sulfate transporters reveal functional subtypes.
Three plant sulfate transporter cDNAs have been isolated by complementation of a yeast mutant with a cDNA library derived from the tropical forage legume Stylosanthes hamata. Two of these cDNAs, shst1 and shst2, encode high-affinity H+/sulfate cotransporters that mediate the uptake of sulfate by plant roots from low concentrations of sulfate in the soil solution. The third, shst3, represents a different subtype encoding a lower affinity H+/sulfate cotransporter, which may be involved in the internal transport of sulfate between cellular or subcellular compartments within the plant. The steady-state level of mRNA corresponding to both subtypes is subject to regulation by signals that ultimately respond to the external sulfate supply. These cDNAs represent the identification of plant members of a family of related sulfate transporter proteins whose sequences exhibit significant amino acid conservation in filamentous fungi, yeast, plants, and mammals.
Smith
F W
FW
Division of Tropical Crops and Pastures, Commonwealth Scientific and Industrial Research Organization, Cunningham Laboratory, St Lucia Old, Australia.
Ealing
P M
PM
Hawkesford
M J
MJ
Clarkson
D T
DT
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D018076:DNA, Complementary; D007887:Fabaceae / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D015723:Gene Library; D017343:Genes, Plant; D005816:Genetic Complementation Test; D026901:Membrane Transport Proteins; D008961:Models, Structural; D008969:Molecular Sequence Data; D010802:Phylogeny; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D018749:RNA, Plant / Q000032:analysis / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid; D000076988:Sulfate Transporters; D013431:Sulfates / Q000378:metabolism
D002352:Carrier Proteins; D018076:DNA, Complementary; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C085609:SLC26A4 protein, human; D000076988:Sulfate Transporters; D013431:Sulfates