title: Filtered technetium-99m-sulfur colloid evaluated for lymphoscintigraphy.
abstract: Several 99mTc-labeled radiopharmaceuticals have been developed for lymphoscintigraphy of the extremities. In the United States, however, these agents are not widely used clinically. This study evaluates the use of smaller particle sizes ( < 0.1 micron) of 99mTc-sulfur colloid (99mTc-SC) for lymphoscintigraphy.
  
  
  METHODS
  The 99mTc-SC was prepared by kit, and the final preparation was filtered through a sterile 0.1-micron filter. The radiochemical purity (RCP) of the filtered 99mTc-SC was determined before administration. Nineteen patients with suspected lymphedema were injected with 18.5 MBq (500 muCi) filtered 99mTc-SC intradermally in each foot, and whole-body images were obtained immediately and 1, 3, 6 and 24 hr later. Local views over the inguinal or axillary lymph nodes were also obtained every 5 min for the first hour.
  
  
  RESULTS
  The average RCP value was 93.4% +/- 4.2% (n = 19), and the RCP difference pre- and postfiltration of the 99mTc-SC preparation was -1.7% +/- 1.4% (n = 40). Evaluation of the particle size with the polycarbonate filter showed that 89.9% +/- 4.5% (n = 28) of particles were less than 50 nm, and the particle size was further determined by electron microscopy to be 38.0 +/- 3.3 nm (n = 202). The mean particle sizes of two peaks measured by laser light scattering techniques were 7.5 and 53.9 nm (major peak). Clinical studies with filtered 99mTc-SC demonstrated similar lymphoscintigrams compared with those obtained with 99mTc antimony sulfide colloid (99mTc-ATC). Filtered 99mTc-SC showed a faster transport rate to the inguinal lymph nodes and lower radiation dosimetry for liver, spleen and whole body compared with 99mTc-ATC.
  
  
  CONCLUSIONS
  Filtered 99mTc-SC can be easily prepared and is readily available for routine clinical use in lymphoscintigraphic studies.
authors.affiliation: Department of Diagnostic Radiology, Mayo Clinic, Rochester, Minnesota 55905, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Hung
authors.forename: G A
authors.initials: GA
authors.lastname: Wiseman
authors.forename: H W
authors.initials: HW
authors.lastname: Wahner
authors.forename: B P
authors.initials: BP
authors.lastname: Mullan
authors.forename: T R
authors.initials: TR
authors.lastname: Taggart
authors.forename: W L
authors.initials: WL
authors.lastname: Dunn
mesh_terms: D000965:Antimony; D003102:Colloids; D005374:Filtration; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008198:Lymph Nodes / Q000000981:diagnostic imaging*; D008209:Lymphedema / Q000000981:diagnostic imaging*; D010316:Particle Size; D011877:Radionuclide Imaging; D011933:Reagent Kits, Diagnostic; D017556:Technetium Compounds; D013671:Technetium Tc 99m Sulfur Colloid
chemical_list: D003102:Colloids; D011933:Reagent Kits, Diagnostic; D017556:Technetium Compounds; C019096:technetium Tc 99m antimony sulfide; D013671:Technetium Tc 99m Sulfur Colloid; D000965:Antimony