title: Screening of polyacetylenic alcohols in crude drugs using the ELISA for panaxytriol.
abstract: Polyacetylenic alcohols such as panaxytriol, panaxynol and panaxydol isolated from the roots of Panax ginseng C. A. MEYER have antiproliferative activity against various cultured tumor cells. Anti-panaxytriol antibody was obtained by immunizing rabbits with panaxytriol-bovine serum albumin conjugates. Although the antibody reactivity was directed mainly toward panaxytriol, there was a slight cross-reactivity with other polyacetylenic compounds. The antibody was, therefore, used for screening a large number of crude drugs for polyacetylenic compounds such as panaxytriol. Methanol-extracts from 31 crude drugs were examined. Significant reactivity was observed in 15 methanol-extracts from Aralieaceae, Compositae and Umbelliferae as reported by other investigators. Three out of the 15 crude drugs were selected for determination of the potent cross-reactive compounds. Four kinds of cross-reactive compounds were isolated by silica gel column chromatography, monitoring each fraction using the enzyme-linked immunosorbent assay (ELISA). Among them, panaxynol and heptadeca-1,8-diene-4,6-diyne-3,10-diol were identified from Saposhnikoviae Radix. Falcarindiol was newly identified from Peucedani Radix. A new polyacetylenic alcohol, 9,10-epoxy-16-hydroxy-octadeca-17-ene-12,14-diyne-1-al, was also isolated from Foeniculi Fructus. All these polyacetylenic alcohols inhibited the growth of a human gastric adenocarcinoma cell line, MK-1 cells, in a dose-dependent manner. These results indicate that the antibody against panaxytriol is an effective tool for "screening" antiproliferative polyacetylenic compounds.
authors.affiliation: Faculty of Hospital Pharmacy, Saga Medical School, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Saita
authors.forename: M
authors.initials: M
authors.lastname: Katano
authors.forename: H
authors.initials: H
authors.lastname: Matsunaga
authors.forename: I
authors.initials: I
authors.lastname: Kouno
authors.forename: H
authors.initials: H
authors.lastname: Fujito
authors.forename: M
authors.initials: M
authors.lastname: Mori
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy; D000480:Alkynes; D000818:Animals; D000918:Antibody Specificity; D000972:Antineoplastic Agents, Phytogenic / Q000032:analysis* / Q000627:therapeutic use; D003429:Cross Reactions; D004354:Drug Screening Assays, Antitumor; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000627:therapeutic use; D053281:Enediynes; D004797:Enzyme-Linked Immunosorbent Assay; D005233:Fatty Alcohols / Q000032:analysis* / Q000627:therapeutic use; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013055:Spectrophotometry, Infrared; D013274:Stomach Neoplasms / Q000188:drug therapy; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay
chemical_list: D000480:Alkynes; D000972:Antineoplastic Agents, Phytogenic; D004365:Drugs, Chinese Herbal; D053281:Enediynes; D005233:Fatty Alcohols; C057682:panaxytriol