In vivo treatment of Heymann's Nephritis using a cytotoxic protein-toxin conjugate.
In this study we investigated the possibility of treating Heymann's Nephritis (HN) by destroying antibody producing cells by targetting a toxin, gelonin--conjugated to gp330, the renal brush border antigen. HN was induced in rats by immunizing them with purified gp330. The gelonin-gp330 conjugate was administered 12 days after the antigenic challenge. Serum was screened for circulating antibodies. Proteinurea was estimated. The gp330-gelonin conjugate-treated animals had a circulating antibody titre in the serum much lower than that of diseased (untreated) animals. Proteinurea seen in diseased animals was not observed in treated animals. This work suggests the possibility of using a toxin-antigen conjugate for immunomodulating antibody mediated autoimmune renal disease.
Misquith
S
S
Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.
Surolia
A
A
D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D005921:Glomerulonephritis / Q000628:therapy*; D000922:Immunotoxins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D007672:Kidney Cortex / Q000276:immunology*; D008715:Methionine / Q000378:metabolism; D008871:Microvilli / Q000276:immunology; D010940:Plant Proteins / Q000627:therapeutic use*; D014176:Protein Biosynthesis / Q000187:drug effects; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D012156:Reticulocytes / Q000187:drug effects / Q000378:metabolism; D054789:Ribosome Inactivating Proteins, Type 1
D000922:Immunotoxins; D010940:Plant Proteins; D011500:Protein Synthesis Inhibitors; D054789:Ribosome Inactivating Proteins, Type 1; C025674:GEL protein, Gelonium multiflorum; D008715:Methionine