title: Template integrity is essential for PCR amplification of 20- to 30-kb sequences from genomic DNA.
authors.affiliation: Department of Human Genetics, Roche Molecular Systems, Inc., Alameda, California 94501, USA.
authors.forename: S
authors.initials: S
authors.lastname: Cheng
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: J A
authors.initials: JA
authors.lastname: Monforte
authors.forename: R
authors.initials: R
authors.lastname: Higuchi
authors.forename: B
authors.initials: B
authors.lastname: Van Houten
mesh_terms: D001483:Base Sequence; D002852:Chromatography, Ion Exchange / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000235:genetics / Q000302:isolation & purification; D003956:Dialysis; D004587:Electrophoresis, Agar Gel; D005260:Female; D015894:Genome, Human; D005914:Globins / Q000235:genetics; D006801:Humans; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D005810:Multigene Family; D010920:Placenta; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial; D007202:Indicators and Reagents; D005914:Globins; D004247:DNA