Study of retinoic acid effect upon retinoic acid receptors beta (RAR-beta) in C6 cultured glioma cells.
Using monoclonal antibodies against the RAR-alpha and RAR-beta retinoic receptors, we demonstrated that these receptors were present together in C6 glioma cells as two isoforms of 50 and 55 kDa. For RAR-beta, the 50 kDa isoform predominated (60 to 80% of the total of the two isoforms). After a treatment for 48 h with retinoic acid 10 microM, the 55 kDa form was enhanced, while no effect was observed either on RAR-alpha isoforms from C6 cells and on both RAR-alpha and RAR-beta forms from neuroblastoma SKN SH SY5Y used as a control. Using purified neuronal and glial rat brain nuclei, we showed that the 55 kDa isoform from RAR-beta predominated in glial cells. These results suggest that retinoic acid treatment of C6 cells led to a partial differentiation, the enhancement of the heavy form of RAR-beta being a marker of this phenomenon.
Reboul
P
P
Laboratoire de Biochimie Générale et Médicale, INSERM-CNRS U.189 Faculté de Médecine Lyon-Sud, Oullins, France.
George
P
P
Louisot
P
P
Broquet
P
P
D000818:Animals; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D002467:Cell Nucleus / Q000737:chemistry / Q000201:enzymology; D005910:Glioma / Q000737:chemistry*; D008970:Molecular Weight; D009447:Neuroblastoma / Q000737:chemistry; D009457:Neuroglia / Q000737:chemistry*; D009474:Neurons / Q000737:chemistry / Q000187:drug effects; D051381:Rats; D018168:Receptors, Retinoic Acid / Q000032:analysis* / Q000737:chemistry; D000072482:Retinoic Acid Receptor alpha; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D015415:Biomarkers; C000607073:Rara protein, rat; D018168:Receptors, Retinoic Acid; D000072482:Retinoic Acid Receptor alpha; C082925:retinoic acid receptor beta; D014212:Tretinoin