title: Eosinophil cationic protein in serum and nasal washes from wheezing infants and children.
abstract: OBJECTIVE
  To compare eosinophil counts and concentrations of eosinophil cationic protein (ECP) in serum and nasal wash fluid from wheezing infants and children with those from age-matched children without respiratory tract symptoms.
  
  
  METHODS
  A case-control study of 71 children treated for wheezing and 59 control subjects in the University of Virginia Pediatric Emergency Department. The patients ranged from 2 months to 16 years of age. Eosinophil numbers and ECP concentrations were assessed in serum and nasal washes. Total serum IgE was measured and the radioallergosorbent test was used to measure IgE antibody to common inhalant allergens.
  
  
  RESULTS
  Among children less than the age of 2 years, markedly elevated levels of ECP (> 200 ng/ml) were measured in nasal washes from 9 (41%) of 22 wheezing patients and 1 (6%) of 17 control subjects (p < 0.03). None of these children had a positive radioallergosorbent test result for IgE antibody to common aeroallergens or a nasal smear containing 10% eosinophils. Few of the wheezing children under 2 years of age had either increased concentrations of total IgE or ECP in their serum or an elevated total blood eosinophil count. After the age of 2 years, the percentage of patients with nasal ECP levels greater than 200 ng/ml was also significantly higher in wheezing children than in control subjects (p < 0.001), and a positive correlation was observed between ECP concentrations in their nasal washes and other eosinophil responses (total blood eosinophil counts, serum ECP levels, and nasal eosinophil counts).
  
  
  CONCLUSIONS
  Increased concentrations of ECP were detected in nasal washes from wheezing infants and children, indicating that eosinophils may contribute to the pathogenesis of airway inflammation in some children who wheeze early in life.
authors.affiliation: Department of Pediatrics, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Ingram
authors.forename: G P
authors.initials: GP
authors.lastname: Rakes
authors.forename: G E
authors.initials: GE
authors.lastname: Hoover
authors.forename: T A
authors.initials: TA
authors.lastname: Platts-Mills
authors.forename: P W
authors.initials: PW
authors.lastname: Heymann
mesh_terms: D000293:Adolescent; D001798:Blood Proteins / Q000032:analysis; D002412:Cations / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D004804:Eosinophils; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D007223:Infant; D017817:Nasal Lavage Fluid / Q000737:chemistry*; D012135:Respiratory Sounds; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification; D012140:Respiratory Tract Diseases / Q000821:virology
chemical_list: D001798:Blood Proteins; D002412:Cations; D007073:Immunoglobulin E