title: An enzyme-linked immunosorbent assay (ELISA) for monitoring rodent colonies for Pasteurella pneumotropica antibodies.
abstract: An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Pasteurella pneumotropica antibodies in the sera of rats, mice, hamsters and Mastomys. P. pneumotropica from mice and rats showed cross-reactivity. The ELISA using P. pneumotropica NCTC 8284 detected more infected animals than selective culture in groups of rodents from which P. pneumotropica, Haemophilus sp and/or Actinobacillus sp were cultured. Cross reactivity between P. pneumotropica NCTC 8284 and haemophilus and actinobacillus isolates were not studied.
authors.affiliation: Section of Laboratory Animal Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Boot
authors.forename: H C
authors.initials: HC
authors.lastname: Thuis
authors.forename: J L
authors.initials: JL
authors.lastname: Veenema
authors.forename: R G
authors.initials: RG
authors.lastname: Bakker
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D006224:Cricetinae; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D010325:Pasteurella / Q000276:immunology* / Q000302:isolation & purification; D010326:Pasteurella Infections / Q000175:diagnosis / Q000662:veterinary*; D015661:Pasteurellaceae / Q000302:isolation & purification; D016871:Pasteurellaceae Infections / Q000175:diagnosis / Q000662:veterinary*; D051381:Rats; D012376:Rodent Diseases / Q000175:diagnosis*; D013045:Species Specificity
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera