Two novel rat guanylin molecules, guanylin-94 and guanylin-16, do not increase cyclic GMP production in T84 cells.
Guanylin, a 15-amino acid peptide homologue of bacterial heat-stable enterotoxins, is an endogenous activator of guanylate cyclase C (GC-C). We isolated two novel guanylin molecules from rat intestinal mucosa. They contained guanylin-15 at their C-termini and were identified as guanylin-94 and guanylin-16 by amino acid sequencing and mass spectrometry. Guanylin-94 and guanylin-16 in total account for 85% of guanylin molecules in both the small and large intestine, guanylin-15 being a minor component. Rat guanylin-94 and guanylin-16 did not increase cyclic GMP production in T84 cells. Identification of the post-translational processing products of guanylin should provide a better understanding of the biosynthesis of the peptide.
Yamaguchi
H
H
Third Department of Medicine, Miyazaki Medical College, Japan.
Nakazato
M
M
Miyazato
M
M
Kangawa
K
K
Matsuo
H
H
Matsukura
S
S
D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003106:Colon; D006152:Cyclic GMP / Q000378:metabolism*; D005768:Gastrointestinal Hormones; D007082:Ileum; D036341:Intercellular Signaling Peptides and Proteins; D007413:Intestinal Mucosa / Q000737:chemistry; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D045265:Natriuretic Peptides; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D011499:Protein Processing, Post-Translational; D051381:Rats; D017207:Rats, Sprague-Dawley
D005768:Gastrointestinal Hormones; D036341:Intercellular Signaling Peptides and Proteins; D045265:Natriuretic Peptides; D010455:Peptides; C095867:guanylin 16; C095868:guanylin 94; C072685:guanylin; D006152:Cyclic GMP; D015056:1-Methyl-3-isobutylxanthine