title: Myt1: a membrane-associated inhibitory kinase that phosphorylates Cdc2 on both threonine-14 and tyrosine-15.
abstract: Cdc2 is the cyclin-dependent kinase that controls entry of cells into mitosis. Phosphorylation of Cdc2 on threonine-14 and tyrosine-15 inhibits the activity of the enzyme and prevents premature initiation of mitosis. Although Wee1 has been identified as the kinase that phosphorylates tyrosine-15 in various organisms, the threonine-14-specific kinase has not been isolated. A complementary DNA was cloned from Xenopus that encodes Myt1, a member of the Wee1 family that was discovered to phosphorylate Cdc2 efficiently on both threonine-14 and tyrosine-15. Myt1 is a membrane-associated protein that contains a putative transmembrane segment. Immunodepletion studies suggested that Myt1 is the predominant threonine-14-specific kinase in Xenopus egg extracts. Myt1 activity is highly regulated during the cell cycle, suggesting that this relative of Wee1 plays a role in mitotic control.
authors.affiliation: Division of Biology 216-76, Howard Hughes Medical Institute, California Institute of Technology, Pasadena 91125, USA.
authors.forename: P R
authors.initials: PR
authors.lastname: Mueller
authors.forename: T R
authors.initials: TR
authors.lastname: Coleman
authors.forename: A
authors.initials: A
authors.lastname: Kumagai
authors.forename: W G
authors.initials: WG
authors.lastname: Dunphy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002462:Cell Membrane / Q000201:enzymology; D003001:Cloning, Molecular; D016213:Cyclins / Q000378:metabolism; D007399:Interphase; D008938:Mitosis; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins; D009865:Oocytes / Q000201:enzymology; D010766:Phosphorylation; D010769:Phosphothreonine / Q000378:metabolism; D019000:Phosphotyrosine / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014981:Xenopus; D029867:Xenopus Proteins
chemical_list: D018797:Cell Cycle Proteins; D016213:Cyclins; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D029867:Xenopus Proteins; D010769:Phosphothreonine; D019000:Phosphotyrosine; C095970:MYT1 kinase, Xenopus; C530005:WEE1 protein, Xenopus; D011505:Protein-Tyrosine Kinases; D017346:Protein Serine-Threonine Kinases; D016203:CDC2 Protein Kinase