title: Differential regulation of motor neuron survival and choline acetyltransferase expression following axotomy.
abstract: Although it is well known that motor neuron survival following axotomy is enhanced with maturation, the ability of surviving neurons to express the cholinergic enzyme choline acetyltransferase (ChAT) following axotomy has not ben closely examined. Moreover, the utility of the facial nucleus in studies of motoneuron response to injury and to trophic factors, coupled with the increasing importance of the mouse in gene targeting, compelled us to investigate the age dependence of neuronal survival and ChAT expression in the mouse facial nucleus following axotomy. We cut the facial nerve at postnatal day (P) 4, 7, 14, 21, and 28 or in the adult and used Nissl staining and ChAT immunocytochemistry to quantitate survival and ChAT expression, respectively, following 1, 2, or 3 weeks' survival at each age. We confirm in this model that the rate and extent of motor neuron death following axotomy is reduced with increasing maturity. The surviving neurons maintain a high ChAT content through P21; however, axotomy from P28 through adulthood results in a striking reduction in ChAT immunoreactivity. That is, although axotomy at P21 results in 61% motor neuron survival, with virtually all of the surviving neurons being ChAT positive, axotomy in the adult results in 72% survival but only 9% of the neurons are ChAT positive. Thus, surviving motor neurons in the adult animals are only weakly cholinergic. These results indicate that a change in the regulation of ChAT expression occurs following P21 so that cell survival and enzyme levels are uncoupled. We suggest that the putative factor or factors that enhances motor neuron survival in maturity is not capable of maintaining ChAT expression.
authors.affiliation: Biology Division, California Institute of Technology, Pasadena 91125, USA.
authors.forename: S Y
authors.initials: SY
authors.lastname: Kou
authors.forename: A Y
authors.initials: AY
authors.lastname: Chiu
authors.forename: P H
authors.initials: PH
authors.lastname: Patterson
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D001369:Axons / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D002795:Choline O-Acetyltransferase / Q000032:analysis*; D005154:Facial Nerve / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009046:Motor Neurons / Q000166:cytology*
chemical_list: D002795:Choline O-Acetyltransferase