title: Hox genes and growth: early and late roles in limb bud morphogenesis.
abstract: In recent years, molecular analysis has led to the identification of some of the key genes that control the morphogenesis of the developing embryo. Detailed functional analysis of these genes is rapidly leading to a new level of understanding of how embryonic form is regulated. Understanding the roles that these genes play in development can additionally provide insights into the evolution of morphology. The 5' genes of the vertebrate Hox clusters are expressed in complex patterns during limb morphogenesis. Various models suggest that the Hoxd genes specify positional identity along the anteroposterior (A-P) axis of the limb. Close examination of the pattern of Hoxd gene expression in the limb suggests that a distinct combination of Hoxd gene expressed in different digit primordia is unlikely to specify each digit independently. The effects of altering the pattern of expression of the Hoxd-11 gene at different times during limb development indicate that the Hoxd genes have separable early and late roles in limb morphogenesis. In their early role, the Hoxd genes are involved in regulating the growth of the undifferentiated limb mesenchyme. Restriction of the expression of successive 5' Hoxd genes to progressively more posterior regions of the bud results in the asymmetric outgrowth of the limb mesenchyme. Later in limb development, Hoxd genes also regulate the maturation of the nascent skeletal elements. The degree of overlap in function between different Hoxd genes may be different in these early and late roles. The combined action of many Hox genes on distinct developmental processes contribute to pattern asymmetry along the A-P axis.
authors.affiliation: Cutaneous Biology Research Center, Charlestown, MA 02129, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Morgan
authors.forename: C
authors.initials: C
authors.lastname: Tabin
mesh_terms: D000818:Animals; D002642:Chick Embryo; D005121:Extremities / Q000196:embryology*; D015870:Gene Expression; D005801:Genes, Homeobox / Q000502:physiology*; D008648:Mesoderm / Q000502:physiology*; D009024:Morphogenesis / Q000235:genetics; D010641:Phenotype; D014714:Vertebrates / Q000196:embryology / Q000235:genetics*