title: Depressed transient outward current in single hypertrophied cardiomyocytes isolated from the right ventricle of ferret heart.
abstract: OBJECTIVE
  The aim of this study was to investigate transient outward potassium current (Ito) changes as a basis for the prolongation of the action potential associated with cardiac hypertrophy.
  
  
  METHODS
  Right ventricular hypertrophy was induced by chronic pulmonary artery constriction in adult male ferrets. After 4-6 weeks, hearts were excised and single myocytes were isolated from the right ventricles of banded and sham-operated (control) animals by enzymatic dissociation. Ito was recorded by means of the whole cell patch clamp technique.
  
  
  RESULTS
  Heart weight:body weight ratio and cell membrane capacitance, as indications of hypertrophy, were increased by 17% (P < 0.05) and 32% (P < 0.01) respectively in the banded group. Analysis of Ito showed that in hypertrophied myocytes compared to normal controls: (1) the density of current was significantly reduced; (2) both the time to peak and the time constant of inactivation were increased; (3) the voltage-dependent steady-state availability was not changed, with similar potentials for half activation (30.4 +/- 6.8 mV in control and 33.9 +/- 8.5 mV in hypertrophied cells) and half inactivation (-12.3 +/- 3.3 mV in control and -11.4 +/- 2.7 mV in hypertrophied cells); (4) the time constant for recovery from inactivation was significantly increased regardless of the holding potentials (-50 mV, -70 mV or -90 mV).
  
  
  CONCLUSIONS
  Alterations of the transient outward potassium current with respect to its density, kinetics and recovery from inactivation can explain the prolongation of the action potential in myocytes isolated from pressure-overload hypertrophied heart and may thus be an important step in such cardiac adaptation.
authors.affiliation: Laboratory of General Physiology, URA CNRS 1869, University of Poitiers, France.
authors.forename: D
authors.initials: D
authors.lastname: Potreau
authors.forename: J P
authors.initials: JP
authors.lastname: Gomez
authors.forename: N
authors.initials: N
authors.lastname: Fares
mesh_terms: D000200:Action Potentials; D000818:Animals; D001693:Biological Transport, Active; D004195:Disease Models, Animal; D005289:Ferrets; D017380:Hypertrophy, Right Ventricular / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D018408:Patch-Clamp Techniques; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels; D013997:Time Factors
chemical_list: D015221:Potassium Channels; D011188:Potassium