title: Role of airway eosinophils in the development of allergen-induced airway hyperresponsiveness in dogs.
abstract: The role of the eosinophil in the development of allergen-induced airway hyperresponsiveness is uncertain. We examined whether the development of airway hyperresponsiveness in 17 dogs after inhalation of Ascaris suum allergen (10(-6) to 10(-2) weight/volume [w/v]) was associated with increases in the number and level of activation of eosinophils before and after allergen inhalation. Airway responsiveness to inhaled acetylcholine was measured before and 24 h after Ascaris inhalation. Eosinophil number was assessed by bronchoalveolar lavage performed 1 wk before allergen inhalation and 15 min after the 24 h acetylcholine challenge. Dogs that developed Ascaris-induced airway hyperresponsiveness (n = 8) had a significantly greater number of bronchoalveolar lavage eosinophils before allergen inhalation (mean +/- SEM: 4.6 +/- 1.94 x 10(4) cells/ml) than dogs that did not become hyperresponsive (n = 9) (1.2 +/- 0.81 x 10(4) cells/ml) (p = 0.03). Ascaris-induced airway hyperresponsiveness, measured 24 h after allergen inhalation, was not associated with increases in eosinophil number after allergen challenge. These results suggest that the presence of airway eosinophils before allergen inhalation is necessary for the development of allergen-induced airway hyperresponsiveness.
authors.affiliation: Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
authors.forename: M J
authors.initials: MJ
authors.lastname: Woolley
authors.forename: C G
authors.initials: CG
authors.lastname: Lane
authors.forename: R
authors.initials: R
authors.lastname: Ellis
authors.forename: W H
authors.initials: WH
authors.lastname: Stevens
authors.forename: K L
authors.initials: KL
authors.lastname: Woolley
authors.forename: P M
authors.initials: PM
authors.lastname: O'Byrne
mesh_terms: D000109:Acetylcholine / Q000008:administration & dosage; D000403:Airway Resistance; D000485:Allergens / Q000276:immunology*; D000704:Analysis of Variance; D000818:Animals; D017165:Ascaris suum / Q000276:immunology; D001980:Bronchi / Q000276:immunology*; D016535:Bronchial Hyperreactivity / Q000209:etiology* / Q000503:physiopathology; D001985:Bronchial Provocation Tests / Q000706:statistics & numerical data; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004285:Dogs; D004804:Eosinophils / Q000276:immunology*; D007958:Leukocyte Count; D011897:Random Allocation; D012882:Skin Tests; D018709:Statistics, Nonparametric
chemical_list: D000485:Allergens; D000109:Acetylcholine