Immunohistochemical reference ranges for B lymphocytes in bone marrow biopsy paraffin sections.
The quantitation of B lymphocytes in paraffin-decalcified bone marrow sections is often used as supportive evidence of primary or residual B-cell lymphoproliferative disorders; however, well-defined normal ranges for B lymphocytes are not available. Thirty-four B5-fixed decalcified bone marrow sections were analyzed from a group of patients having an essentially normal bone marrow morphologic examination and lacking any evidence immunologic or hematopoietic disease. All samples were stained with CD20, CD45RA (4KB5), and DBA.44 for B cells, and CD45RO (UCHL-1) for T cells. Image analysis was performed on all cases. CD20-positive cells ranged from 0% to 5.97%. CD45RA positivity ranged from 0% to 5.48%. DBA.44 was positive in 0% to 2.07% of the cells. CD45RO positivity varied from 0% to 6.7%. These results provide useful immunohistochemical B-lymphocyte reference ranges in B5-fixed paraffin bone marrow sections.
O'Donnell
L R
LR
Saint Mary's Medical Center, Saginaw, Michigan, USA.
Alder
S L
SL
Balis
U J
UJ
Perkins
S L
SL
Kjeldsberg
C R
CR
D000328:Adult; D000368:Aged; D001402:B-Lymphocytes; D001707:Biopsy, Needle; D001854:Bone Marrow Cells; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010232:Paraffin; D012016:Reference Values
D010232:Paraffin