title: Molecular characterization of fusion regulatory protein-1 (FRP-1) that induces multinucleated giant cell formation of monocytes and HIV gp160-mediated cell fusion. FRP-1 and 4F2/CD98 are identical molecules.
abstract: Fusion regulatory protein (FRP)-1 regulates virus-mediated cell fusion and fusion of monocytes. Eleven of fifteen N-terminal amino acids of FRP-1 were the same as the amino acid sequence of 4F2/CD98 heavy chain. FRP-1 molecules were detected in Con A- or IL-2-stimulated lymphocytes, while FRP-1 was rare on resting lymphocytes. These properties of FRP-1 are similar to those of 4F2/CD98. Treatment of monocytes with anti-4F2/CD98 mAbs resulted in cell fusion, and other mAbs directed against 4F2/CD98 induced formation of multinucleated giant cells of Cd+U2ME-7 cells, a CD4+U937 cell line transfected with the HIV gp160 gene. Both anti-4F2/CD98 and anti-FRP-1 mAbs reacted with murine L929 cells expressing human 4F2/CD98 transiently or constitutively. When Newcastle disease virus (NDV)-infected L929 cells expressing human FRP-1/CD98 were incubated with mAb 4-5-1, an anti-FRP-1 mAb, multinucleated giant cells were induced; thus, FRP-1/CD98 molecules expressed in L929 cells are functional for fusion regulatory activity.
authors.affiliation: Department of Microbiology, Mie University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ohgimoto
authors.forename: N
authors.initials: N
authors.lastname: Tabata
authors.forename: S
authors.initials: S
authors.lastname: Suga
authors.forename: M
authors.initials: M
authors.lastname: Nishio
authors.forename: H
authors.initials: H
authors.lastname: Ohta
authors.forename: M
authors.initials: M
authors.lastname: Tsurudome
authors.forename: H
authors.initials: H
authors.lastname: Komada
authors.forename: M
authors.initials: M
authors.lastname: Kawano
authors.forename: N
authors.initials: N
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Ito
mesh_terms: D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000737:chemistry* / Q000276:immunology; D001483:Base Sequence; D002459:Cell Fusion / Q000187:drug effects; D027261:Fusion Regulatory Protein-1; D015686:Gene Products, env / Q000494:pharmacology*; D015726:Giant Cells / Q000166:cytology / Q000276:immunology*; D019302:HIV Envelope Protein gp160; D006367:HeLa Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000737:chemistry / Q000166:cytology* / Q000276:immunology; D011498:Protein Precursors / Q000494:pharmacology*; D014760:Viral Fusion Proteins / Q000737:chemistry* / Q000276:immunology
chemical_list: D015703:Antigens, CD; D027261:Fusion Regulatory Protein-1; D015686:Gene Products, env; D019302:HIV Envelope Protein gp160; D011498:Protein Precursors; D014760:Viral Fusion Proteins