Serial analysis of gene expression.
The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.
Velculescu
V E
VE
Oncology Center, Johns Hopkins University, Baltimore, MD 21231, USA.
Zhang
L
L
Vogelstein
B
B
Kinzler
K W
KW
D001483:Base Sequence; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D005821:Genetic Techniques; D006801:Humans; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes