[Preliminary studies on microbe-mediated N-nitrosamide synthesis].
Chemical synthesis of N-nitrosamide and influences on it by bacteria and fungi isolated from human gastric juice were studied with trace analysis method to explore the pathway of its endogenous formation. Results showed in synthesis of N-methyl-N-nitrosourea (MNU) with precursors of methylurea (0.42 mmol/L) and sodium nitrite (25 mmol/L) in a condition simulating human stomach, pH of the reaction system played an important role in the synthesis, the amount of synthetic chemical maximized at a pH of 1-3 and almost no synthesis was found at pH of 5-7. A study on the ability to synthesize MNU catalyzed by bacteria isolated from the patients with stomach cancer living in a high-risk area showed its synthesis could be accelerated by Pseudomonas aeruginosa at pH of 6-7 with its catalyzing activity existed in heat liable component of living bacteria. This is the first report showing microorganisms to catalyze the formation of N-nitrosamide from its precursors.
Pan
K
K
Beijing Institute for Cancer Research, Beijing Medical University.
Deng
D
D
Li
T
T
D005750:Gastric Juice / Q000382:microbiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008770:Methylnitrosourea / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification
D008770:Methylnitrosourea