Hepatic indices of thyroid status in rats treated with hexachlorobenzene.
The functional thyroid status of hexachlorobenzene (HCB)-treated rats was studied. HCB caused a depletion of serum thyroxine (T4), but did not change L-3,5,3'-triiodothyronine (T3) levels in serum of rats. The activities of the thyroid regulated mitochondrial enzyme L-glycerolphosphate dehydrogenase (LGPD) and cytosolic enzymes, malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) were assayed in livers of normal and HCB (100 mg/100 g bw) treated Wistar rats. Mitochondrial LGPD activity did not change significantly, however ME, 6GPD and G6PD were induced by HCB only in non-thyroidectomized animals. The absence of cytosolic enzymes induction in thyroidectomized rats treated with HCB indicates that HCB is not intrinsically thyromimetic. The induction of hepatic ME, G6PD and 6PGD activities in HCB thyroidectomized rats was dependent on the presence of thyroid hormone. The unchanged activity of mitochondrial LGPD in contrast to the increased activities of the cytosolic enzymes ME, G6PD and 6PGD is not consistent with a shift in functional thyroid status following HCB treatment.
Kleiman de Pisarev
D L
DL
Departamentos de Quimica Biológica, Facultad de Medicina, Buenos Aires, Argentina.
Ferramola de Sancovich
A M
AM
Sancovich
H A
HA
D000818:Animals; D001835:Body Weight / Q000502:physiology; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006581:Hexachlorobenzene / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology / Q000502:physiology*; D008291:Malate Dehydrogenase / Q000378:metabolism; D009929:Organ Size / Q000502:physiology; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013961:Thyroid Gland / Q000502:physiology*; D013963:Thyroid Hormones / Q000096:biosynthesis / Q000097:blood
D013963:Thyroid Hormones; D006581:Hexachlorobenzene; D005993:Glycerolphosphate Dehydrogenase; D008291:Malate Dehydrogenase; C030574:malate dehydrogenase (decarboxylating); D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase