Chromosomal position effects and the modulation of transgene expression.
Chromosomal position effects can influence strongly the transcription of foreign genes in transgenic animals. This results in low frequencies and levels of gene expression and, in some cases, in aberrant patterns of expression. Strategies for overcoming these effects are described with particular reference to their application in embryonic stem cells.
Clark
A J
AJ
AFRC Roslin Institute, Midlothian, Scotland, UK.
Bissinger
P
P
Bullock
D W
DW
Damak
S
S
Wallace
R
R
Whitelaw
C B
CB
Yull
F
F
D000818:Animals; D002875:Chromosomes; D004274:DNA, Recombinant; D004622:Embryo, Mammalian; D015870:Gene Expression; D018390:Gene Targeting; D018014:Gene Transfer Techniques; D006801:Humans; D013234:Stem Cells
D004274:DNA, Recombinant