title: Carrier-mediated lactate entry into isolated hepatocytes from fed and starved rats: zonal distribution and temperature dependence.
abstract: We examined the possibility of quantitative differences in lactate entry into periportal and perivenous hepatocytes under different nutritional states. The rate of 14C-L(+)-lactate uptake was determined after 15-second incubations with freshly isolated zonally separated hepatocytes using a centrifuge stop technique at 37 degrees C and 4 degrees C, in the presence or absence of either differing amounts of unlabelled lactate or of a hepatocyte lactate transport inhibitor, alpha-cyano-3-hydroxycinnamate. Total entry as well as carrier mediated entry of 14C-L(+)-lactate into the isolated cell populations was found to be similar in periportal and perivenous hepatocytes, irrespective of the nutritional state of the animal. Periportal and perivenous hepatocytes showed a greater tendency to transport lactate when isolated from starved animals, in agreement with previously reported data from non-zonally separated isolated hepatocytes. The activity of the hepatocyte plasma-membrane lactate transporter was diminished between fourfold and eightfold in transport studies conducted at 4 degrees C; similar results were obtained in unseparated and zonally separated suspensions. Temperature dependence of the hepatocyte transporter is markedly less than that reported for the erythrocyte transporter.
authors.affiliation: University of Bristol, Department of Biochemistry, School of Medical Sciences, U.K.
authors.forename: M A
authors.initials: MA
authors.lastname: Staricoff
authors.forename: R D
authors.initials: RD
authors.lastname: Cohen
authors.forename: J P
authors.initials: JP
authors.lastname: Monson
mesh_terms: D000818:Animals; D001692:Biological Transport / Q000502:physiology; D002250:Carbon Radioisotopes; D002352:Carrier Proteins / Q000378:metabolism; D003373:Coumaric Acids; D002149:Energy Intake / Q000502:physiology*; D006503:Hepatic Veins / Q000166:cytology; D007773:Lactates / Q000378:metabolism*; D019344:Lactic Acid; D008099:Liver / Q000166:cytology* / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013696:Temperature
chemical_list: D002250:Carbon Radioisotopes; D002352:Carrier Proteins; D003373:Coumaric Acids; D007773:Lactates; D019344:Lactic Acid; C018254:alpha-cyano-3-hydroxycinnamate