Molecular cloning of 25-hydroxyvitamin D-3 24-hydroxylase (Cyp-24) from mouse kidney: its inducibility by vitamin D-3.
A cDNA encoding a 25-hydroxyvitamin D-3 24-hydroxylase, Cyp-24, has been isolated from mouse kidney cDNA library by hybridization screening. Mouse Cyp-24, coding for 514 amino acid residues, shared 82.1 and 94.7% amino acid identity with human and rat CYP24s, respectively. Among mouse organs examined, Cyp-24 mRNA could be detected in the kidney. When mice were treated with vitamin D-3, Cyp-24 mRNA was induced in the kidney.
Itoh
S
S
Division of Bio-Medical and Immunological Chemistry, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
Yoshimura
T
T
Iemura
O
O
Yamada
E
E
Tsujikawa
K
K
Kohama
Y
Y
Mimura
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002762:Cholecalciferol / Q000494:pharmacology*; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004790:Enzyme Induction; D005786:Gene Expression Regulation / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification; D013250:Steroid Hydroxylases / Q000096:biosynthesis / Q000235:genetics*; D065668:Vitamin D3 24-Hydroxylase
D018076:DNA, Complementary; D012333:RNA, Messenger; D002762:Cholecalciferol; D003577:Cytochrome P-450 Enzyme System; D013250:Steroid Hydroxylases; D065668:Vitamin D3 24-Hydroxylase