Structural studies on a cell wall polysaccharide from Bifidobacterium longum YIT4028.
The major fraction of rhamnogalactan was isolated from the cell wall of Bifidobacterium longum YIT4028 by treatment with N-acetylmuramidase. This polysaccharide was composed of rhamnose and galactose in a molar ratio of about 2:3. The 13C NMR spectrum indicated that it contained a pentasaccharides repeating unit. This observation, and the results of Smith degradation, partial acid hydrolysis and methylation analysis led to the conclusion that its structure is [formula: see text]
Nagaoka
M
M
Yakult Central Institute for Microbiological Research, Tokyo, Japan.
Shibata
H
H
Kimura
I
I
Hashimoto
S
S
Kimura
K
K
Sawada
H
H
Yokokura
T
T
D001644:Bifidobacterium / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D002850:Chromatography, Gel; D005690:Galactose / Q000032:analysis / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012210:Rhamnose / Q000032:analysis / Q000737:chemistry
D011135:Polysaccharides, Bacterial; D012210:Rhamnose; D005690:Galactose