Molecular cloning and sequencing of cytochrome c' from the phototrophic purple sulfur bacterium Chromatium vinosum.
The gene for cytochrome c' from Chromatium vinosum was cloned from a HindIII-SalI digest of genomic DNA. A 1.4 kbp fragment containing the gene was sequenced in both directions using the Sanger dideoxy method. The cytochrome c' gene codes for a 154-residue peptide, of which the last 131 amino acids match the previously determined sequence of the protein. The remaining 23 residues represent a signal sequence that is cleaved from the polypeptide upon translocation to the periplasmic space. An additional open reading frame on the other strand of the fragment codes for a peptide that contains four regions that are homologous to corresponding regions of the cytochrome b-type subunit of several Ni-Fe hydrogenases.
Even
M T
MT
Department of Chemistry, University of Illinois at Chicago 60607, USA.
Kassner
R J
RJ
Dolata
M
M
Meyer
T E
TE
Cusanovich
M A
MA
D000595:Amino Acid Sequence; D001483:Base Sequence; D002844:Chromatium / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000737:chemistry / Q000235:genetics*; D004269:DNA, Bacterial; D008969:Molecular Sequence Data
D003574:Cytochrome c Group; D004269:DNA, Bacterial