1,25-Dihydroxycholecalciferol stimulates the expression of intercellular adhesion molecule-1 in renal carcinoma cells.
1,25-Dihydroxycholecalciferol (1,25-D3) is a potent immunomodulatory vitamin modulating major histocompatibility complex class-II expression in monocytes and epithelial cells. However, the impact of 1,25-D3 on the expression of adhesion molecules in epithelial cells has not been investigated. Human renal tubular epithelial and renal carcinoma cells express intercellular adhesion molecule-1 (ICAM-1), a ligand of the leukocyte-function-associated antigen-1 (LFA-1). Therefore, we addressed the question whether 1,25-D3 modulates ICAM-1 expression by renal carcinoma cells. Using an enzyme-linked immunoassay we detected an increase in ICAM-1 expression by a renal carcinoma cell line (ACHN) cultured in the presence of 1,25-D3. Also, in ACHN cells stimulated with gamma-IFN a significant stimulatory effect of 1,25-D3 was evident. ICAM-1 is crucial for the adhesion of LFA-1-expressing lymphocytes and is involved in antigen presentation. In addition, ICAM-1 may be of significance in lymphocyte lysis of tumor cells. Therefore, the impact of 1,25-D3 on ICAM-1 expression by renal and non-renal and non-renal carcinoma cells and renal tubular epithelial cells may be of clinical importance.
Kreft
B
B
Department of Internal Medicine, Medical University of Lübeck, Germany.
Brzoska
S
S
Müller
F
F
Doehn
C
C
Sack
K
K
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology; D007680:Kidney Neoplasms / Q000378:metabolism*; D051379:Mice; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D011994:Recombinant Proteins; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma; D002117:Calcitriol