CD27/CD70 interaction directly drives B cell IgG and IgM synthesis.
CD27 is a T cell activation antigen expressed on a majority of peripheral blood T cells. CD27 is also expressed on a subpopulation of human B cells, and it is reported that CD27+ B cells secrete both IgG and IgM. CD70, a ligand for CD27, is expressed on activated T and B cells, suggesting an interaction between T and B cells via CD27/CD70 ligation. Here, we analyze B cell immunoglobulin synthesis using a CD70 transfectant and present functional data showing that B cells secrete large amounts of IgG and IgM as a result of the CD27/CD70 interaction. A flow cytometric analysis showed that CD27 expression was increased and CD70 was expressed on tonsillar and peripheral blood B cells after activation with Staphylococcus aureus Cowan strain (SAC) plus interleukin (IL-2). In addition, the proliferation of B cells was enhanced mildly by the addition of CD70 transfectant, and its proliferation was blocked by anti-CD70 mAb. More importantly, the CD70 transfectant enhanced IgG and IgM production by purified B cells greatly in the presence of SAC plus IL-2. The enhancement was completely blocked by the addition of either anti-CD70 mAb or anti-CD27 mAb. Strongly suggesting that the interaction of CD27 with its ligand, CD70, on B cells plays an important role in B cell growth and differentiation to produce IgG and IgM.
Agematsu
K
K
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.
Kobata
T
T
Yang
F C
FC
Nakazawa
T
T
Fukushima
K
K
Kitahara
M
M
Mori
T
T
Sugita
K
K
Morimoto
C
C
Komiyama
A
A
D000328:Adult; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D001773:Blood Cells; D053282:CD27 Ligand; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000494:pharmacology; D008024:Ligands; D008213:Lymphocyte Activation / Q000187:drug effects; D008211:Lymphocyte Cooperation; D008565:Membrane Proteins / Q000235:genetics / Q000276:immunology*; D014066:Palatine Tonsil / Q000166:cytology; D011994:Recombinant Proteins / Q000378:metabolism; D013211:Staphylococcus aureus / Q000276:immunology; D014162:Transfection; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D053282:CD27 Ligand; C505771:CD70 protein, human; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007376:Interleukin-2; D008024:Ligands; D008565:Membrane Proteins; D011994:Recombinant Proteins; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7