Characterization of a new antigen expressed by B and myeloid lineage cells identified by the monoclonal antibody LIP-6.
The LIP-6 MAb was produced against the undifferentiated cell line bh2-1 and recognizes an antigen expressed on all pre-B and B cell lines tested and some myeloid lineage lines. FACS analysis of normal tissues showed that LIP-6 is expressed on B lineage cells at all stages of differentiation, from bone marrow pre-B to plasma cells. T cells and thymocytes are LIP-6-, and splenic CD11b+ cells are heterogeneous for LIP-6 expression. The LIP-6 MAb was shown to precipitate a major 75-kDa and a minor 85-kDa protein under reducing conditions and a large protein of > 240 kDa under nonreducing conditions. Removal of N-linked sugars from the reduced lysates resulted in a single 65-kDa protein, suggesting that there is differential glycosylation of a single 65-kDa protein that forms disulfide-linked multimers. Finally, the LIP-6 antigen was shown not to be linked to the cell surface via a GPI linkage.
Holmes
K L
KL
Laboratory of Molecular Structure, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA.
Lantz
L M
LM
Lee
J S
JS
Bedigian
H G
HG
Taubenberger
J K
JK
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D000937:Antigen-Antibody Reactions; D000943:Antigens, Differentiation / Q000737:chemistry / Q000276:immunology*; D001402:B-Lymphocytes / Q000737:chemistry / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D002460:Cell Line; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010529:Peritoneal Cavity / Q000166:cytology; D010950:Plasma Cells / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000276:immunology; D014018:Tissue Distribution / Q000276:immunology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation