[Enzymatic synthesis of dihydroxyethylthiamine pyrophosphate, its optical characteristics, and quantitative determination].
Modification of the method of Holzer et al. for the synthesis and purification of dihydroxyethylthiamine pyrophosphate, an intermediate of the transketolase reaction, is described. Absorption and circular dichroism spectra as well as the molar coefficient of circular dichroism absorption at 273 nm are presented. A convenient and rapid procedure for quantitative determination of dihydroxyethylthiamine pyrophosphate in solution where it is present as the only component or as a mixture with thiamine pyrophosphate (or other optically inactive compounds), is proposed.
Sidorova
N N
NN
Usmanov
R A
RA
Kochetov
G A
GA
D000818:Animals; D002942:Circular Dichroism; D011768:Pyruvate Dehydrogenase Complex / Q000737:chemistry*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013552:Swine; D013835:Thiamine Pyrophosphate / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis
D011768:Pyruvate Dehydrogenase Complex; D012996:Solutions; C093420:dihydroxyethylthiamine pyrophosphate; D013835:Thiamine Pyrophosphate
Enzimaticheskiĭ sintez digidroksiétiltiaminpirofosfata, ego opticheskie kharakteristiki i kolichestvennoe opredelenie.