Lymphoproliferative activity of methimazole: free SH group dependency.
1. The comparative effects of methimazole (MTI), an antithyroid drug, and its S-methyl derivate (MMTI), were studied in vitro on the lymphoproliferative response to lectin in order to point out the free SH group importance. The cell cycle analysis was performed by flow cytometry after cellular DNA staining by propidium iodide. 2. We showed that MTI enhanced the PHA-induced DNA synthesis phase (P < 0.05 from 1 to 100 microns) whereas MMTI had no significant activity. The free SH group seems to be necessary to the MTI immunomodulatory activity.
Chabernaud
M L
ML
Department of Organic and Therapeutic Chemistry, Faculty of Pharmacy of the University of Limoges, France.
Fatimi
J
J
Lagorce
J F
JF
Segure
F
F
Buxeraud
J
J
Raby
C
C
D000328:Adult; D002453:Cell Cycle; D002478:Cells, Cultured / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects*; D008713:Methimazole / Q000378:metabolism / Q000494:pharmacology*
D037102:Lectins; D008713:Methimazole