title: Chemoprotective effects of capsaicin and diallyl sulfide against mutagenesis or tumorigenesis by vinyl carbamate and N-nitrosodimethylamine.
abstract: Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide) is a major pungent and irritating ingredient of hot chilli peppers, which are frequently consumed as spices. This dietary phytochemical has been found to interact with microsomal xenobiotic metabolizing enzymes in rodents. Capsaicin and its saturated analog dihydrocapsaicin (trans-8-methyl-N-vanillyl-6-nonanamide) have been proposed to inactivate cytochrome P-450 HE1 by irreversibly binding to the active sites of the enzyme. Besides cytochrome P-450 HE1, other isoforms of the P-450 superfamily were also reported to be inhibited by capsaicin. The inhibition by capsaicin of microsomal monooxygenases involved in carcinogen activation implies its chemopreventive potential. As part of a program to investigate chemoprotective properties of capsaicin we initially determined the effect of capsaicin on vinyl carbamate (VC)- and N-nitrosodimethylamine (NDMA)-induced mutagenesis in Salmonella typhimurium TA100. Capsaicin (0.42 mM) attenuated the bacterial mutagenicity of VC and NDMA by 50% and 42% respectively. Diallyl sulfide, a thioether found in garlic with selective P-450 HE1 inhibitory activity, also lessened the mutagenicity of the above carcinogens in a concentration-dependent manner. The suppression of VC- and NDMA-induced mutagenesis by capsaicin and diallyl sulfide correlated with their inhibition of P-450 IIE1-mediated p-nitrophenol hydroxylation and NDMA N-demethylation. Pretreatment of female ICR mice with a topical dose of capsaicin lowered the average number of VC-induced skin tumors by 62% at 22 weeks after promotion. A similar degree of protection was attained with oral administration of diallyl sulfide before carcinogen treatment. The results of this study suggest that capsaicin and diallyl sulfide suppress VC- and NDMA-induced mutagenesis or tumorigenesis in part through inhibition of the cytochrome P-450 IIE1 isoform responsible for activation of these carcinogens.
authors.affiliation: Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT 06520-8034, USA.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Surh
authors.forename: R C
authors.initials: RC
authors.lastname: Lee
authors.forename: K K
authors.initials: KK
authors.lastname: Park
authors.forename: S T
authors.initials: ST
authors.lastname: Mayne
authors.forename: A
authors.initials: A
authors.lastname: Liem
authors.forename: J A
authors.initials: JA
authors.lastname: Miller
mesh_terms: D000498:Allyl Compounds; D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002211:Capsaicin / Q000031:analogs & derivatives / Q000494:pharmacology / Q000627:therapeutic use*; D002273:Carcinogens / Q000494:pharmacology / Q000633:toxicity*; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004128:Dimethylnitrosamine / Q000633:toxicity*; D004221:Disulfiram / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D051379:Mice; D008813:Mice, Inbred ICR; D009152:Mutagenicity Tests; D009153:Mutagens / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects; D012878:Skin Neoplasms / Q000139:chemically induced* / Q000517:prevention & control*; D013440:Sulfides / Q000494:pharmacology / Q000627:therapeutic use*; D014520:Urethane / Q000031:analogs & derivatives* / Q000633:toxicity
chemical_list: D000498:Allyl Compounds; D016588:Anticarcinogenic Agents; D016587:Antimutagenic Agents; D002273:Carcinogens; D065607:Cytochrome P-450 Enzyme Inhibitors; D009153:Mutagens; D013440:Sulfides; D014520:Urethane; C038491:allyl sulfide; C017963:vinyl carbamate; D003577:Cytochrome P-450 Enzyme System; D004128:Dimethylnitrosamine; D002211:Capsaicin; D004221:Disulfiram; C012906:dihydrocapsaicin