[Effect of acetylcholine on the proliferation of T lymphocyte of rat spleen].
Experiments were performed to determine the effect of various concentrations of acetylcholine (ACh) (10(-10) to 10(-4) mol/L) on the proliferation of spleen cells of rat to concanavalin (Con A). ACh at 10(-9) mol/L to 10(-4) mol/L range significantly enhanced the proliferation of T cells, reaching a maximum at 10(-7) and 10(-6) mol/L. The proliferation of lymphocytes first stimulated by various concentrations of ACh (10(-9), 10(-7) or 10(-5) mol/L) for one hour and incubated with Con A for 72 h still showed increase. When ACh was washed off for one hour, the proliferative enhancement of lymphocytes to Con A remained to occur. However, ACh did not enhance the proliferation of T cells which were once stimulated with Con A for 6 h. Atropine at 10(-7) or 10(-6) mol/L abolished completely the effect of ACh at 10(-9) or 10(-5) mol/L. The action of ACh at 10(-7) mol/L could be blocked by 10(-6) mol/L atropine. These data suggest that ACh at a wide range of concentrations can enhance cellular immunity, an effect occurring either before or after T cells are activated through muscarinic cholinergic receptors.
Qiu
Y H
YH
Department of Physiology, Nantong Medical College.
Peng
Y P
YP
Zhang
Q Q
QQ
Wang
J H
JH
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D005260:Female; D008297:Male; D015213:Neuroimmunomodulation / Q000187:drug effects; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects*
D018377:Neurotransmitter Agents; D001285:Atropine; D000109:Acetylcholine