title: Proferrioxamine synthesis in Erwinia amylovora in response to precursor or hydroxylysine feeding: metabolic profiling with liquid chromatography-electrospray mass spectrometry.
abstract: Metabolic profiling by capillary liquid chromatography-electrospray mass spectrometry was used to monitor shifts in the proferrioxamine profiles of Erwinia amylovora in response to externally supplied potential proferrioxamine precursors, selected stable-isotope-labeled precursors and atypical precursors. Based on the qualitative and quantitative shifts in the proferrioxamine profiles, lysine and arginine are unambiguous, and agmatine, ornithine, diaminobutyric acid and the corresponding C3-5 diamines are highly likely precursors for proferrioxamine biosynthesis in E. amylovora. 5-Hydroxylysine (Hyl), a recently discovered growth inhibitor for E. amylovora, suppresses proferrioxamine production. The Hyl-induced growth inhibition can be reversed by basic amino acids. The basic amino acids also partly restore proferrioxamine synthesis.
authors.affiliation: Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA.
authors.forename: G J
authors.initials: GJ
authors.lastname: Feistner
mesh_terms: D002853:Chromatography, Liquid; D003676:Deferoxamine / Q000378:metabolism*; D004885:Erwinia / Q000378:metabolism*; D005290:Ferric Compounds / Q000378:metabolism*; D013058:Mass Spectrometry; D011492:Protein Hydrolysates / Q000378:metabolism*
chemical_list: D005290:Ferric Compounds; D011492:Protein Hydrolysates; C007978:hydrolysin; C002577:ferrioxamine B; D003676:Deferoxamine