title: Membrane potential and intercellular calcium during glucose challenge in mouse islet of Langerhans.
abstract: The present work was undertaken to study the effects of the addition and removal of glucose on the calcium concentration of the intercellular space [Ca2+]o of islets of Langerhans. The removal of glucose hyperpolarize the beta-cell membrane to near- 60 mV and induced a biphasic response in [Ca2+]o. On the other hand, the addition of glucose (11 mM) depolarized beta-cell, inducing a biphasic electrical activity and concomitant changes in the [Ca2+]o. During the continuous membrane electrical activity, which characterized the first phase of glucose evoked biphasic response, the [Ca2+]o decreased. In the second phase, the [Ca2+]o displayed an oscillatory behavior concomitantly with the membrane potential.
authors.affiliation: Department of Physiology, University of State of Rio de Janeiro, Brazil.
authors.forename: A S
authors.initials: AS
authors.lastname: Moura
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005071:Evoked Potentials / Q000187:drug effects; D005110:Extracellular Space / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D013997:Time Factors
chemical_list: D005947:Glucose; D002118:Calcium