title: Jejunal absorption of trypsin in rat and guinea pig.
abstract: Gut absorption is one of the first requirements for the study of the mechanism of a possible anti-inflammatory action of proteases, such as orally administered trypsin. Porcine trypsin absorption was studied in isolated jejunal loops of rats (female Holtzman and male Wistar) and guinea pigs (males) by open-loop perfusion. Trypsin was dissolved in Tyrode solution and the solution perfused at a rate of 0.5 ml/min, at 37 degrees C. Trypsin activity, total protein, and sodium and potassium concentrations were assayed in the jejunal effluent; the values were unchanged throughout the experiments, which lasted 45 to 120 min. Using a high sensitivity ELISA (i.e. pg/ml), trypsin absorption could be demonstrated by determination of the enzyme in the mesenteric venous blood (samples of 0.5 ml); the enzyme concentration increased with time of perfusion. The linear range-specificity for intact trypsin varied from 1 to 500 ng/well. In this assay polyclonal antibodies prepared against trypsin-TLCK were utilized. Whereas trypsin concentration in the perfused lumen was practically constant at 0.12 mg/ml, the concentration of absorbed trypsin in mesenteric vein blood increased from about 100 ng/ml at time zero to 1.8 micrograms/ml, after 45 min of perfusion. Histological and ultrastructural examination of the jejunal mucosa before and after perfusion revealed that the brush-border, basal membrane, and junctional complexes were fully preserved, thus eliminating the possibility that trypsin might have destroyed the structures, thereby reaching the blood circulation. The present data indicate that micrograms quantities of trypsin were absorbed by the isolated jejunal loop of the rat.
authors.affiliation: Departamento de Fisiologia e Biofísica, Universidade de Minas Gerais, Belo Horizonte, Brasil.
authors.forename: E L
authors.initials: EL
authors.lastname: Borges
authors.forename: A
authors.initials: A
authors.lastname: Petroianu
authors.forename: A J
authors.initials: AJ
authors.lastname: Barbosa
authors.forename: E R
authors.initials: ER
authors.lastname: Camargos
authors.forename: S C
authors.initials: SC
authors.lastname: Guatimosim
authors.forename: M
authors.initials: M
authors.lastname: Mares-Guia
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006168:Guinea Pigs; D007408:Intestinal Absorption; D007583:Jejunum / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D010477:Perfusion / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D014357:Trypsin / Q000032:analysis / Q000378:metabolism*
chemical_list: D014357:Trypsin