[The role of disulfide bridges of the residual protein in chromosomal DNA organization].
The review of literature data and our investigation on the role of disulfide bridges of residual protein (RP) in structural organization of chromosomal DNA is presented. It was studied the action of several S-S cleaving agents (2-mercaptoethanol, dithiothreitol, NaBH4, glutathione reductase) on native DNA-RP complexes, isolating from the different eukaryotic and prokaryotic cells. It was shown, that the thiols result the fragmentation of DNA-RP complex in double-strand subunits of several size (5 x 10(5), (18-20) x 10(6), 70 x 10(6) Da) on dependence of the incubate condition (concentration of thiols, pH, time). It was observed, that specific S-S bonds (thiol-sensitivity at neutral or acid conditions, glutathione reductase-sensitivity) are present in DNA-RP complexes, which may control of different structural levels of DNA into chromosome (gen-transcription-replicon-domain). The possible quasisubunit structure of chromosomal DNA with participation of polypeptide S-S bonds and complementary "sticky" ends of subunits is discussed.
Struchkov
V A
VA
Strzhevskaia
N B
NB
Blokhin
D Iu
DIu
D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure*; D004247:DNA / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry*; D008854:Microscopy, Electron; D011506:Proteins / Q000737:chemistry*
D004220:Disulfides; D011506:Proteins; D004247:DNA
Rol' disul'fidnykh mostikov ostatochnogo belka v organizatsii khromosomal'noĭ DNK.