Glucose induces oscillations of cytoplasmic Ca2+, Sr2+ and Ba2+ in pancreatic beta-cells without participation of the thapsigargin-sensitive store.
Individual pancreatic beta -cells were used to study the glucose effects on the handling of Ca2+, Sr2+ and Ba2+. In extracellular medium containing one of these ions, single beta -cells responded to 11 mM glucose with large amplitude oscillations in cytoplasmic Ca2+, Sr2+ or Ba2+ with indistinguishable average frequencies (0.30-0.33/min). The oscillations disappeared after hyperpolarization with 400 microM diazoxide. Under such hyperpolarization, glucose stimulated the sequestration of Ca2+ and Sr2+ but not of repetitively mobilized by consecutive exposures to 100 microM carbachol. A 2-3 min exposure to 100 nM of the intracellular Ca(2+)-ATPase inhibitor thapsigargin also mobilized Ca2+ and Sr2+ and irreversibly abolished subsequent release by carbachol. However, thapsigargin did not prevent the large amplitude oscillations in Ca2+, Sr2+ or Ba2+ under non-hyperpolarizing conditions although the frequency of the Ca2+ oscillations was almost doubled. The results indicate that the slow oscillatory behavior of glucose-stimulated individual beta -cells does not depend on inositol 1,4,5-trisphosphate mediated release of intracellular Ca2+.
Liu
Y J
YJ
Department of Medical Cell Biology, Uppsala University, Sweden.
Grapengiesser
E
E
Gylfe
E
E
Hellman
B
B
D000818:Animals; D001464:Barium / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors*; D002413:Cations, Divalent / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008820:Mice, Obese; D013324:Strontium / Q000378:metabolism; D013729:Terpenes / Q000494:pharmacology*; D019284:Thapsigargin
D002413:Cations, Divalent; D013729:Terpenes; D001464:Barium; D019284:Thapsigargin; D000252:Calcium-Transporting ATPases; D005947:Glucose; D002118:Calcium; D013324:Strontium