title: Roa307, a protein encoded on Coxiella burnetii plasmid QpH1, shows homology to proteins encoded in the replication origin region of bacterial chromosomes.
abstract: TnphoA mutagenesis identified an open reading frame, roa307, immediately upstream of the partition locus qsopAB on the Coxiella burnetii plasmid QpH1. The protein sequence deduced from roa307 displayed homology to Orf290 of Pseudomonas putida, Orf283 and Orf282 (SpoOJ) of Bacillus subtilis-hypothetical products of genes in the chromosomal replication origin region. Expression of roa307 was demonstrated by PhoA activity of an Roa307-PhoA fusion.
authors.affiliation: Department of Microbiology, Washington State University, Pullman 99164-4233, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Lin
authors.forename: D
authors.initials: D
authors.lastname: Howe
authors.forename: L P
authors.initials: LP
authors.lastname: Mallavia
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002876:Chromosomes, Bacterial; D016997:Coxiella burnetii / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000032:analysis; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D011993:Recombinant Fusion Proteins; D018741:Replication Origin; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009711:Nucleotides; D011993:Recombinant Fusion Proteins; C095700:Roa307 protein, Coxiella burnetii