title: Pulmonary hypertension in rats. 1. Role of bromodeoxyuridine-positive mononuclear cells and alveolar macrophages.
abstract: The role of activated cells determined by staining with bromodeoxyuridine (BrdU) and the role of alveolar macrophages as a source of cytokines were investigated in monocrotaline (MCT)-induced pulmonary hypertension (PH) in rats. BrdU was mainly incorporated by mononuclear cells; their number increased strikingly prior to the development of PH. Cytokines such as interleukin (IL)-1, IL-6 and tumor necrosis factor were produced during the development of PH. The amount of IL-6 showed significant correlation (p < 0.05) with the number of BrdU-positive cells, which, in turn, correlated with the extent of PH. These results indicate that BrdU-positive cells and cytokines produced by alveolar macrophages may play an important role in the pathogenesis of MCT-induced PH in rats.
authors.affiliation: Department of Internal Medicine II, Fukushima Medical College, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Miyata
authors.forename: F
authors.initials: F
authors.lastname: Sakuma
authors.forename: A
authors.initials: A
authors.lastname: Yoshimura
authors.forename: H
authors.initials: H
authors.lastname: Ishikawa
authors.forename: T
authors.initials: T
authors.lastname: Nishimaki
authors.forename: R
authors.initials: R
authors.lastname: Kasukawa
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D006976:Hypertension, Pulmonary / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000473:pathology*; D016676:Macrophages, Alveolar / Q000276:immunology / Q000473:pathology*; D008297:Male; D016686:Monocrotaline / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016207:Cytokines; D016686:Monocrotaline; D001973:Bromodeoxyuridine