title: Gradient of integrin alpha 6A distribution in the myocardium during early heart development.
abstract: The interactions of cells with extracellular matrices (ECM)1 are likely to be key determinants of embryonic development. Integrin adhesion receptors are ideally positioned to mediate some of these interactions since, in addition to mechanical adhesion, they transduce signals affecting cell proliferation and differentiation. We investigated expression of the integrin alpha 6 beta 1, a receptor for the ECM component, laminin in the early mouse embryo. An intriguing feature of this integrin is the existence of alpha 6 subunit isoforms. The A and B isoforms, which differ in the cytoplasmic tails, are expressed in cell-type specific fashion, and are likely to implement distinct cellular interactions with laminin. By RT-PCR, alpha 6B but not alpha 6A mRNA was detectable in embryo extracts from fertilized oocytes to 6.5 d.p.c. In subsequent stages, up to 11.5 d.p.c., alpha 6A mRNA was observed in mRNA extracts from whole embryos, but still in significantly lower amounts than alpha 6B. However, in extracts from isolated heart (9.5 to 11.5 d.p.c.), alpha 6A was the predominant alpha 6 isoform, while in extracts from other embryo parts no alpha 6A mRNA was detectable. At the protein level, immunostaining with specific antibodies showed alpha 6A protein in myocardial cells, at the early stage of heart tube development (8.5 d.p.c.). Localization to the myocardium was tightly restricted, since other structures of the embryonic heart, e.g., endocardium, or of the remaining embryo did not stain with anti-alpha 6A antibody. In the ventricular myocardium, expression of alpha 6A appeared more intense than in the subendocardial layer. Quantitation by confocal microscopy unveiled a gradient of expression of alpha 6A, increasing from the outer to the inner layers of the myocardium. This is the first demonstration of a gradient distribution of integrin molecules in a tissue, which appears to be directly connected with the process of organogenesis. The mechanism underlying our observations is not the turning on of a gene, rather it is the activation of a splicing mechanism that substitutes the cytoplasmic domain of a laminin receptor. Because integrin cytoplasmic domains are thought to be an important functional end of the molecule, this may be a mechanism to modulate cellular responses to laminin.
authors.affiliation: Department of Cell Biology, Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: G
authors.initials: G
authors.lastname: Collo
authors.forename: S Z
authors.initials: SZ
authors.lastname: Domanico
authors.forename: G
authors.initials: G
authors.lastname: Klier
authors.forename: V
authors.initials: V
authors.lastname: Quaranta
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis*; D001483:Base Sequence; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D017931:DNA Primers; D005314:Embryonic and Fetal Development; D005260:Female; D015870:Gene Expression; D005865:Gestational Age; D006321:Heart / Q000196:embryology* / Q000502:physiology; D039503:Integrin alpha6; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013480:Superovulation
chemical_list: D015703:Antigens, CD; D017931:DNA Primers; D039503:Integrin alpha6; D012333:RNA, Messenger