title: Dexamethasone regulates obese expression in isolated rat adipocytes.
abstract: An obese (ob) gene product, expressed specifically in adipose tissues, regulates energy balance. Here we report adipocytes in adipose tissue actually express ob mRNA and that a synthetic glucocorticoid (dexamethasone) regulates expression of the ob gene. Addition of 100 nM dexamethasone to isolated rat adipocytes rapidly induced a 4-8 fold increase in ob mRNA. This increase in ob mRNA level was apparent within 1 h, and reached a maximum at about 7 h after stimulation. The dexamethasone-stimulated increase of ob mRNA was only partially blocked by protein synthesis inhibitors cycloheximide (20 micrograms/ml) or anisomycin (200 microM). This suggests that new protein synthesis is not necessarily required for the observed dexamethasone-stimulated increase in ob mRNA.
authors.affiliation: Department of Laboratory Medicine, School of Medicine, University of Tokushima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Murakami
authors.forename: M
authors.initials: M
authors.lastname: Iida
authors.forename: K
authors.initials: K
authors.lastname: Shima
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D002454:Cell Differentiation; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D004822:Epididymis / Q000098:blood supply; D015870:Gene Expression / Q000187:drug effects*; D008297:Male; D051379:Mice; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009765:Obesity / Q000235:genetics*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011500:Protein Synthesis Inhibitors; D012333:RNA, Messenger; D000841:Anisomycin; D003907:Dexamethasone; D003513:Cycloheximide; D015056:1-Methyl-3-isobutylxanthine