Electrophoretic polymorphism in rabbit tear lysozyme.
Rabbit tears were found to contain two lysozymes which differed in their electrophoretic mobility and were designated tear lysozymes 1 and 2. Rabbit tear lysozyme 1 was purified to homogeneity by conventional purification methods. It was found to be distinct from other known mammalian c-type lysozymes, rabbit tear lysozyme 2 and the major rabbit gastrointestinal lysozyme. The activity profile is centered around the neutral region with an optimum of 7 which is slightly lower than that for chicken lysozyme. The thermal stability as well as inhibition profiles by the substrate analogues, N-acetylglucosamine (NAG) and chitotetraose (NAG)4 are comparable to those of chicken lysozyme. Based on its molecular weight and catalytic properties this isozyme is classified as a c-type lysozyme.
Saleh
A M
AM
Department of Biological Sciences, Faculty of Science, University of Jordan, Amman.
Ibrahimi
I M
IM
D000818:Animals; D000917:Antibody Formation; D002384:Catalysis; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D005260:Female; D007527:Isoenzymes / Q000235:genetics* / Q000302:isolation & purification; D008297:Male; D009113:Muramidase / Q000235:genetics* / Q000302:isolation & purification; D011110:Polymorphism, Genetic; D011817:Rabbits / Q000235:genetics* / Q000378:metabolism; D013666:Tears / Q000201:enzymology*
D007527:Isoenzymes; D009113:Muramidase