Abundance and half-life of the distinct oat phytochrome A3 and A4 mRNAs.
Gene-preferential oligonucleotide probes were used to determined the relative abundance and half-lives of distinct oat phytochrome A (PHYA) mRNAs. Oat PHYA mRNAs are highly conserved in the 5'-untranslated region and the coding region, but the 3'-untranslated region has an overall lower sequence conservation and was the source of gene-preferential probes. PHYA3 mRNA was estimated to be ca. 61% of the oat PHYA mRNA pool present in poly(A)+ RNA from dark-grown seedlings. The half-lives for PHYA3 and PHYA4 mRNAs were both estimated to be ca. 30 min, and a similar short half-life was estimated for the average PHYA mRNA. Sequence comparisons of PHYA mRNAs from four grass species identified conserved sequences within the 5'- and 3'-untranslated regions that might be important for PHYA mRNA degradation.
Higgs
D C
DC
Department of Botany, Iowa State University, Ames 50011, USA.
Barnes
L J
LJ
Colbert
J T
JT
D018554:Avena / Q000235:genetics* / Q000528:radiation effects; D001483:Base Sequence; D017124:Conserved Sequence; D006207:Half-Life; D008027:Light; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010834:Phytochrome / Q000235:genetics*; D051942:Phytochrome A; D006109:Poaceae / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000528:radiation effects
D015345:Oligonucleotide Probes; D051942:Phytochrome A; D012333:RNA, Messenger; D010834:Phytochrome