title: Sex differences in the steroidogenic and respiratory electron transport chains in the rat adrenal cortex.
abstract: Both steroid 11 beta-hydroxylation and cholesterol side chain cleavage occur in the mitochondria of adrenocortical cells and they require reducing power in the form of NADPH. There are direct sources of NADPH in rat adrenal mitochondria but another potential source of NADPH is the energy-linked transhydrogenase reaction. This suggests that there is a relationship between the steroidogenic and respiratory chains. We have elaborated upon this relationship by exploring the expression of cytochrome c oxidase (CO) and cytochrome P-45011 beta. We have studied the regulation of one mitochondrial-encoded (COII) and one nuclear-encoded (COIV) subunit. Normal, untreated male rats had higher basal levels of activity of CO in adrenal (255%) and liver (144%) mitochondria, compared to normal, untreated female rats. They also had increased COII (300% and 138%) and COIV (300% and 135%). Cytochrome P-45011 beta levels, however, were lower (48%) in adrenal mitochondria of male rats than those of female rats. Androgen treatment of male rats caused an increase in the activity of CO in the mitochondria of the adrenal gland with the levels being 171% of the corresponding controls. This increase in activity paralleled an increase in the levels of COII and COIV in the adrenal as measured by Western analysis. In contrast, adrenal cytochrome P-45011 beta levels were lower (68%). Androgen treatment caused no significant change in the levels of mRNA's for COII and COIV whereas cytochrome P-45011 beta mRNA was significantly lower than normal.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Biochemistry, University at Buffalo, NY 14214, USA.
authors.forename: F
authors.initials: F
authors.lastname: el-Migdadi
authors.forename: S
authors.initials: S
authors.lastname: Gallant
authors.forename: A C
authors.initials: AC
authors.lastname: Brownie
mesh_terms: D000302:Adrenal Cortex / Q000187:drug effects* / Q000378:metabolism; D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012727:Sex Characteristics; D013252:Steroid 11-beta-Hydroxylase / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000728:Androgens; D013252:Steroid 11-beta-Hydroxylase; D003576:Electron Transport Complex IV