Rapid recovery of rat brain intracellular pH after cardiac arrest and resuscitation.
We studied the intracellular pH in rat cerebral cortex of rats subjected to reversible total cerebral ischemia by cardiac arrest and resuscitation. Brain acidoses was more pronounced during ischemia in hyperglycemic rats (6.21 +/- 0.14) than in normoglycemic rats (6.56 +/- 0.07). Brain tissue lactate accumulated proportionally. Nevertheless, within 5 min of reperfusion, pHi in both normoglycemic and hyperglycemic groups had recovered to baseline levels, i.e. near 7.1-7.2, despite the fact that lactate concentrations were still elevated. These results demonstrate a rapid reversal of ischemic acidosis during recovery from 10 min of cardiac arrest, and suggest that acidosis, per se, may not be responsible for neuronal damage following cardiac arrest and resuscitation, even in hyperglycemic conditions.
LaManna
J C
JC
Department of Neurology, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, USA.
Griffith
J K
JK
Cordisco
B R
BR
Bell
H E
HE
Lin
C W
CW
Pundik
S
S
Lust
W D
WD
D000138:Acidosis / Q000503:physiopathology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D001923:Brain Chemistry / Q000502:physiology*; D016887:Cardiopulmonary Resuscitation; D006323:Heart Arrest / Q000503:physiopathology*; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008297:Male; D009499:Neutral Red; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D007773:Lactates; D010725:Phosphocreatine; D009499:Neutral Red; D019344:Lactic Acid; D000255:Adenosine Triphosphate