Simultaneous determination of malachite green and its metabolite leucomalachite green in eel plasma using post-column oxidation.
A rapid HPLC method with solid-phase extraction (SPE) clean-up for malachite green (MG) and leucomalachite green (LMG) in eel plasma was developed. MG and LMG were extracted with a buffered methanolic solution. The extract was subjected to aromatic sulphonic acid SPE. MG and LMG were eluted from the SPE column with methanol after a treatment with ammonia gas. The reconstituted eluate was analyzed on a Chromspher B column with acetonitrile-ion-pair buffer (pH 4.0) (6:4, v/v) as the mobile phase and detection at 610 nm after post-column oxidation with PbO2. The average recoveries for MG and LMG over the linear range of applicability (20-2500 ng/ml) were 82 +/- 1% and 83 +/- 1%, respectively. The limits of quantification were 5.0 micrograms/l for MG and 0.9 micrograms/l for LMG.
Hajee
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University of Utrecht, Faculty of Veterinary Medicine, Department of the Science of Food of Animal Origin, Netherlands.
Haagsma
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D000814:Aniline Compounds / Q000097:blood*; D000818:Animals; D000891:Anti-Infective Agents, Local / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004524:Eels / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012394:Rosaniline Dyes / Q000378:metabolism* / Q000493:pharmacokinetics
D000814:Aniline Compounds; D000891:Anti-Infective Agents, Local; D012394:Rosaniline Dyes; C005095:malachite green; C015320:leucomalachite green