title: Autocrine/paracrine role of insulin-related growth factors in neurogenesis: local expression and effects on cell proliferation and differentiation in retina.
abstract: Early neurogenesis progresses by an initial massive proliferation of neuroepithelial cells followed by a sequential differentiation of the various mature neural cell types. The regulation of these processes by growth factors is poorly understood. We intend to understand, in a well-defined biological system, the embryonic chicken retina, the role of the insulin-related growth factors in neurogenesis. We demonstrate the local presence of signaling elements together with a biological response to the factors. Neuroretina at days 6-8 of embryonic development (E6-E8) expressed proinsulin/insulin and insulin-like growth factor I (IGF-I) mRNAs as well as insulin receptor and IGF type I receptor mRNAs. In parallel with this in vivo gene expression, E5 cultured neuroretinas synthesized and released to the medium a metabolically radiolabeled immunoprecipitable insulin-related peptide. Furthermore, insulin-related immunoreactive material with a HPLC mobility close to that of proinsulin was found in the E6-E8 vitreous humor. Exogenous chicken IGF-I, human insulin, and human proinsulin added to E6 cultured neuroretinas showed relatively close potencies stimulating proliferation, as determined by [methyl-3H]thymidine incorporation, with a plateau reached at 10(-8) M. These factors also stimulated neuronal differentiation, indicated by the expression of the neuron-specific antigen G4. Thus, insulin-related growth factors, interestingly including proinsulin, are present in the developing chicken retina and appear to play an autocrine/paracrine stimulatory role in the progression of neurogenesis.
authors.affiliation: Department of Cell and Developmental Biology, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Cientificas, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Hernández-Sánchez
authors.forename: A
authors.initials: A
authors.lastname: López-Carranza
authors.forename: C
authors.initials: C
authors.lastname: Alarcón
authors.forename: E J
authors.initials: EJ
authors.lastname: de La Rosa
authors.forename: F
authors.initials: F
authors.lastname: de Pablo
mesh_terms: D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002642:Chick Embryo; D003429:Cross Reactions; D004305:Dose-Response Relationship, Drug; D007328:Insulin / Q000276:immunology / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D008954:Models, Biological; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D009924:Organ Culture Techniques; D011384:Proinsulin / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D011972:Receptor, Insulin / Q000096:biosynthesis / Q000235:genetics; D017451:Receptors, Somatomedin / Q000096:biosynthesis* / Q000235:genetics; D012160:Retina / Q000166:cytology / Q000187:drug effects / Q000196:embryology*; D013002:Somatomedins / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D007328:Insulin; D017451:Receptors, Somatomedin; D013002:Somatomedins; D007334:Insulin-Like Growth Factor I; D011384:Proinsulin; D011972:Receptor, Insulin