Modification of a tunable UV-visible capillary electrophoresis detector for simultaneous absorbance and fluorescence detection: profiling of body fluids for drugs and endogenous compounds.
Using fused-silica optical fibres for fluorescence light collection and bandpass filters for selection of emission wavelengths, a capillary electrophoresis detection cell of a conventional, tunable UV-Vis absorbance detector was adapted for simultaneous fluorescence (at selected emission wavelength) and absorbance (at selected excitation wavelength) detection. Detector performance is demonstrated with the monitoring of underivatized fluorescent compounds in body fluids by micellar electrokinetic capillary chromatography with direct sample injection. Compared with UV absorption detection, fluorescence detection is shown to provide increased selectivity and for selected compounds also up to tenfold higher sensitivity. Examples studied include screening for urinary indole derivatives (tryptophan, 5-hydroxytryptophan, tyrosine, 3-indoxyl sulfate and 5-hydroxyindole-3-acetic acid) and catecholamine metabolites (homovanillic acid and vanillylmandelic acid) and the monitoring of naproxen in serum, quinidine in serum and urine and of salicylate and its metabolites in serum and urine.
Caslavska
J
J
Department of Clinical Pharmacology, University of Berne, Switzerland.
Gassmann
E
E
Thormann
W
W
D002395:Catecholamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis / Q000379:methods*; D016231:Fluorescence Polarization Immunoassay; D006801:Humans; D007211:Indoles / Q000032:analysis* / Q000097:blood / Q000652:urine; D009288:Naproxen / Q000032:analysis / Q000097:blood / Q000652:urine; D011802:Quinidine / Q000097:blood / Q000652:urine; D012459:Salicylates / Q000097:blood / Q000652:urine; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D002395:Catecholamines; D007211:Indoles; D012459:Salicylates; D009288:Naproxen; D011802:Quinidine