Typing of Staphylococcus epidermidis colonizing in human nares by pulsed-field gel electrophoresis.
From the nares of 11 healthy adults, 253 strains of coagulase negative staphylococcus were isolated and 88% of them were identified as Staphylococcus epidermidis using the API STAPH system. Chromosomal DNA fingerprinting of the isolated strains revealed that each person carried multiple types of S. epidermidis in his or her nares. The colonization of the strains was not stable; the types of the isolates changed in the first and the second examinations 5 months apart. The results contrasted with previous findings in which only one strain of S. aureus colonized persistently in the nares of healthy adults.
Hu
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Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukoka, Japan.
Umeda
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A
Amako
K
K
D000328:Adult; D015373:Bacterial Typing Techniques; D002353:Carrier State / Q000453:epidemiology / Q000382:microbiology*; D003030:Coagulase / Q000032:analysis; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D009296:Nasal Cavity / Q000382:microbiology*; D015995:Prevalence; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013212:Staphylococcus epidermidis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
D003030:Coagulase; D004269:DNA, Bacterial