title: [Elaboration of the polymerase chain reaction system for subtype specific differentiation of influenza A virus strains].
abstract: New subtype specific primers for hemagglutinin (H1, H2, H3) of influenza A viruses were selected. The specificity of the primers' performance was tested in a model experiment which used cDNA synthesized on the vRNA from the collection of influenza A viruses (H1, H2, H3 subtypes) as templates. The sensibility of the PCR system was determined on DNA plasmids- and RNA templates (with preliminary reverse transcription) and amounted to 10(2) and 10(5)-10(6) virion particles, respectively.
authors.forename: K V
authors.initials: KV
authors.lastname: Tarasov
authors.forename: S Iu
authors.initials: SIu
authors.lastname: Tsvirkunova
authors.forename: T A
authors.initials: TA
authors.lastname: Bekhtereva
mesh_terms: D004279:DNA, Viral / Q000032:analysis; D006389:Hemagglutinins, Viral; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D013698:Templates, Genetic
chemical_list: D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D012367:RNA, Viral
vernacular_title: Razrabotka sistemy polimeraznoĭ tsepnoĭ reaktsii dlia subtipospetsifichnoĭ differentsiatsii shtammov virusa grippa A.