[Effects of the nitric oxide donor SIN-1 on the membrane potential of mouse neuroblastoma-rat glioma hybrid cells].
The effect of the nitric oxide donor SIN-1 on the membrane potential of cultured mouse neuroblastoma-rat glioma hybrid NG108-15 cells was investigated using the whole cell patch method. It has been reported that neurite formation can be induced in NG108-15 cells by adding of dibutyryl cyclic AMP to the culture medium. Using this system we found that SIN-1 has a selective inhibitory effect on the membrane potential of the calcium current which is concentration-dependent in the 1 mu M-100 microM range. This effect was transient and reversible, the same as seen with the calcium channel blocker nilvadipine at concentrations of 10 microM to 10 microM. At higher concentrations, ranging from 500 microM to 1 mM, however, SIN-1 also caused prolonged inhibition of the membrane potential of the sodium current. However, this effect was also reversible. These findings suggest that SIN-1 has a reversible inhibitory action on the membrane potentials of neurons.
Yamasaki
T
T
Department of Neurosurgery, Shimane Medical University, Izumo, Japan.
Akiyama
Y
Y
Fukuda
M
M
Kimura
Y
Y
Moritake
K
K
Enomoto
K
K
Maeno
T
T
D000818:Animals; D002118:Calcium / Q000378:metabolism; D003864:Depression, Chemical; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D006822:Hybrid Cells; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008981:Molsidomine / Q000031:analogs & derivatives* / Q000494:pharmacology; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D012964:Sodium / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism / Q000502:physiology
D009569:Nitric Oxide; C002385:linsidomine; D012964:Sodium; D008981:Molsidomine; D002118:Calcium