Cloning a cDNA from human NK/T cells which codes for a protein with high proline content.
A cDNA clone, B4-2, was isolated from a natural killer (NK) minus T cell subtractive library. The B4-2 clone coded for an mRNA of 2061 bp in length. It encodes a deduced 327 aa protein with a calculated molecular mass of 35.2 kDa. Searching of B4-2 DNA and protein sequences against various databases revealed no high homology to other sequences. However, B4-2 has an unusually high proline content (13%), contains a putative nuclear targeting sequence, and has several SPXX motifs which are frequently found in gene regulatory proteins. One of the stretches of prolines in B4-2 closely resembles the ligand for proteins with SH3 domains. Northern hybridization data showed that B4-2 is not a lymphoid specific gene and is expressed in a hepatoma cell line and also weakly transcribed or absent in a variety of other cells. A polyclonal antiserum raised against recombinant B4-2 recognizes a 32-34 kDa protein in lymphocytes.
Chen
J
J
Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
Liu
L
L
Pohajdak
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007694:Killer Cells, Natural / Q000737:chemistry*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011392:Proline / Q000032:analysis*; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D011994:Recombinant Proteins / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D009687:Nuclear Proteins; C096395:PNRC1 protein, human; D011506:Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D014157:Transcription Factors; D011392:Proline