A murine model for the immunotherapy of head and neck squamous cell carcinoma.
A murine model for the immunotherapy of head and neck cancer was established. The AT-84 tumor, a spontaneously arising oral squamous cell tumor of C3H mice, was evaluated for susceptibility to lymphokine-activated killer (LAK) cells. In vitro chromium-release assays demonstrated that AT-84 is sensitive to LAK-cell-mediated killing. Furthermore, in vivo experiments employing a lung metastasis model demonstrated a 50% reduction in the number of metastases in LAK-cell-treated mice as compared with untreated controls (P2 = .001). These experiments showed that AT-84 is an appropriate model for the immunotherapy of head and neck cancer. This model should be invaluable for further study of the mechanisms involved in immune-mediated therapy of head and neck cancer.
Hier
M P
MP
Department of Otolaryngology, Lady Davis Institute for Medical Research, McGill University, Montreal, Quebec, Canada.
Black
M J
MJ
Shenouda
G
G
Sadeghi
N
N
Karp
S E
SE
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000628:therapy*; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D004195:Disease Models, Animal; D005260:Female; D006258:Head and Neck Neoplasms / Q000276:immunology / Q000628:therapy*; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007376:Interleukin-2 / Q000008:administration & dosage; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology; D008175:Lung Neoplasms / Q000276:immunology / Q000556:secondary / Q000628:therapy; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D011897:Random Allocation; D018709:Statistics, Nonparametric; D014407:Tumor Cells, Cultured
D007376:Interleukin-2