Sequence of the gene encoding the 16S rRNA of the beer spoilage organism Megasphaera cerevisiae.
The 16S ribosomal RNA gene from the beer-spoilage organism, Megasphaera cerevisiae was polymerase chain reaction (PCR)-amplified and sequenced. Analysis confirmed the phylogenetic position of M. cerevisiae as a sister taxon of Megasphaera elsdenii, within the obligately anaerobic, Gram-negative cocci. The sequence obtained should facilitate the development of DNA probes for early detection of this spoilage organism.
Doyle
L M
LM
Department of Microbiology, University College, Galway, Ireland.
McInerney
J O
JO
Mooney
J
J
Powell
R
R
Haikara
A
A
Moran
A P
AP
D001483:Base Sequence; D001515:Beer / Q000382:microbiology*; D016964:Gram-Negative Anaerobic Cocci / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D016415:Sequence Alignment
D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S