title: Radiolabelling of human haemoglobin using the 125I-Bolton-Hunter reagent is superior to oxidative iodination for conservation of the native structure of the labelled protein.
abstract: Both disruption of the native protein structure and oxidation of iron in the haem/iron(II)-proto-porphyrin-IX residues were observed using the Iodo-gen (1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycouril) method in 125I-labelling of haemoglobin. The reactions taking place affect the native structure of haemoglobin and result in a more acidic molecule. The detrimental effects were unaffected by the presence of iodine. Electrophoretic studies demonstrate that 125I-labelling of haemoglobin using the Bolton-Hunter reagent is the method of choice in order to preserve the native protein.
authors.affiliation: Axis Nord AS, Mørkved, Norway.
authors.forename: F
authors.initials: F
authors.lastname: Frantzen
authors.forename: D E
authors.initials: DE
authors.lastname: Heggli
authors.forename: E
authors.initials: E
authors.lastname: Sundrehagen
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry*; D007457:Iodine Radioisotopes / Q000737:chemistry*; D007525:Isoelectric Focusing; D010084:Oxidation-Reduction; D011487:Protein Conformation; D014508:Urea / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D006454:Hemoglobins; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; C012385:1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril; D014508:Urea