title: Zinc and manganese bioavailability from human milk and infant formula used for very low birthweight infants, evaluated in a rat pup model.
abstract: The bioavailability of zinc and manganese from diets used for very low birthweight infants was investigated in a rat pup model using radioisotopes. The effect of protein source and content and of pasteurization was evaluated, and two different approaches for evaluation of zinc and manganese bioavailability from the studied human milk and infant formula for very low birthweight infants was high. Liver uptake of 65Zn from labeled premature infant diets in sucklings rat pups was 26-29%, and absorption calculated as the difference between administered dose and nonabsorbed activity 6 h after oral intubation was 93-95%. Retention of manganese calculated as the sum of 54Mn retained by organs and carcass was 85-95% from human milk and premature infant formula, and absorption calculated from nonabsorbed activity was 83-88% after 6 h. Fortification of early human milk significantly increased the bioavailability of zinc. No effect of pasteurization of human milk was found on zinc or manganese bioavailability. Liver zinc uptake was found to be a more sensitive parameter than absorption for evaluation of diets with a high zinc bioavailability. Measurement of retained activity of manganese in carcass and organs was judged to be the preferred parameter for evaluation of diets with high manganese availability.
authors.affiliation: Research Department of Human Nutrition, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
authors.forename: E
authors.initials: E
authors.lastname: Knudsen
authors.forename: B
authors.initials: B
authors.lastname: Sandström
authors.forename: O
authors.initials: O
authors.lastname: Andersen
mesh_terms: D000042:Absorption; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001682:Biological Availability; D004305:Dose-Response Relationship, Drug; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007225:Infant Food; D007231:Infant, Newborn; D019102:Infant, Very Low Birth Weight / Q000378:metabolism*; D008297:Male; D008345:Manganese / Q000493:pharmacokinetics*; D008894:Milk Proteins / Q000378:metabolism; D008895:Milk, Human / Q000378:metabolism*; D051381:Rats; D015032:Zinc / Q000493:pharmacokinetics*
chemical_list: D008894:Milk Proteins; D008345:Manganese; D015032:Zinc