title: Insulin inhibits its own secretion from isolated, perifused human pancreatic islets.
abstract: It is still a controversial question whether insulin suppresses its own secretion. We prepared pure human islets from three pancreases by collagenase digestion and density gradient purification. Aliquots of 200 islet equivalents (IE, 150-microns sized-islets) were sequentially perifused at 37 degrees C with 3.3 mmol/l glucose (3.3G, 40 min), 16.7 mmol/l glucose (16.7G, 30 min) and again 3.3G (30 min) after 24 h, 37 degrees C culture in CMRL 1066 medium with or without the addition of either 200 or 400 microU/ml human insulin in the incubation medium (6 replicates each). Insulin secretion was assessed by C-peptide (Cp) measurement in the perifusate. Without added insulin (C) and with 200 (Ins200) or 400 (Ins400) microU/ml added insulin, basal Cp release was 0.12 +/- 0.03, 0.14 +/- 0.02 and 0.14 +/- 0.04 ng/ml, respectively. At 16.7G, the first-phase secretion peak (expressed as Cp value) was significantly lower with Ins200 (0.47 +/- 0.13 ng/ml, P < 0.02) and Ins400 (0.68 +/- 0.15 ng/ml, P < 0.05) than C (0.83 +/- 0.15 ng/ml). The second-phase secretion peak was also significantly (P < 0.05) reduced with added insulin (Ins200: 0.47 +/- 0.08 ng/ml; Ins400: 0.45 +/- 0.07 ng/ml) than in its absence (C: 0.65 +/- 0.09 ng/ml). Accordingly, total Cp secretion was lower with Ins200 (10.6 +/- 2.3 ng/ml, P = 0.03) and Ins400 (11.8 +/- 2.3 ng/ml) than with C (16.0 +/- 2.2 ng/ml).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Metabolic Diseases, University of Pisa, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Marchetti
authors.forename: D W
authors.initials: DW
authors.lastname: Scharp
authors.forename: M
authors.initials: M
authors.lastname: McLear
authors.forename: E H
authors.initials: EH
authors.lastname: Finke
authors.forename: B
authors.initials: B
authors.lastname: Olack
authors.forename: C
authors.initials: C
authors.lastname: Swanson
authors.forename: R
authors.initials: R
authors.lastname: Giannarelli
authors.forename: R
authors.initials: R
authors.lastname: Navalesi
authors.forename: P E
authors.initials: PE
authors.lastname: Lacy
mesh_terms: D002096:C-Peptide / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005246:Feedback; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D010477:Perfusion; D013997:Time Factors
chemical_list: D002096:C-Peptide; D007328:Insulin; D005947:Glucose