Genotoxicity of the herbicide fluchloralin on human lymphocytes in vitro: chromosomal aberration and micronucleus tests.
Cultured human lymphocytes were exposed to three different concentrations (2.5, 5.0 and 10.0 micrograms/ml) of fluchloralin for 24 and 48 h to assess chromosomal aberrations. A significant dose-dependent increase of chromatid type aberration was observed in these cells. Multiple aberrations (MA) were scored at all concentrations after 48 h treatment. To support these results we did micronucleus (MN) test using cytochalasin B to block cytokinesis. At lower concentrations (2.5 to 10.0 micrograms/ml) the frequency of MN induction was not significantly different. Higher concentrations of fluchloralin (20, 40 and 50 micrograms/ml) resulted in a significant dose dependent increase in number of micronucleated cells. This is the first report on genotoxic effects of fluchloralin in human cells.
Panneerselvam
N
N
Cancer Biology Division, School of Biological Sciences, Madurai Kamaraj University, India.
Sinha
S
S
Shanmugam
G
G
D000814:Aniline Compounds / Q000633:toxicity*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006540:Herbicides / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*
D000814:Aniline Compounds; D006540:Herbicides; D009153:Mutagens; C012322:fluchloralin