title: Evaluation of tissue adhesive to contain axonal regeneration in horses.
abstract: Bilateral palmar and plantar digital neurectomies were completed in 10 horses (a total of 80 neurectomies) using one of three methods: (1) simple transection (guillotine method); (2) epineural capping; (3) n-butyl cyanoacrylate injected into the epineural sheath to act as a nerve sealant. Horses were regularly evaluated clinically for tenderness in and around the surgical site, as well as skin sensation at the coronary band in the heel region, during the 12-week course of the study. None of the surgical sites exhibited any signs of drainage or infection. Horses were then euthanatized, the nerve stumps were dissected from surrounding tissues, and the length and width of the tissue mass that had formed on the end of the nerve was recorded. Longitudinal and transverse sections of the nerve endings were examined histologically for numbers of proliferating axon sprouts (neuroma formation); whether the axons had penetrated the epineurium; degree of Schwann cell proliferation; degree of chronic inflammation; extent of foreign body reaction; extent of retrograde degeneration of the nerve bundles; and amount of fibrovascular proliferation. The proportion of legs exhibiting tenderness or heel sensation did not differ significantly between the three different treatments at any of the six different times they were examined. There was no difference between the three treatments in the length or width of the fibrous tissue scar on the ends of the nerves or in the number of sprouting axons from the ends of the nerves. Of 80 nerves examined, only two nerves were not confined to the epineurium.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: Turner
authors.forename: G W
authors.initials: GW
authors.lastname: Trotter
authors.forename: B E
authors.initials: BE
authors.lastname: Powers
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D004341:Drug Evaluation; D004659:Enbucrilate / Q000031:analogs & derivatives* / Q000494:pharmacology; D005528:Foot / Q000294:innervation / Q000601:surgery; D005552:Forelimb / Q000294:innervation / Q000601:surgery; D006614:Hindlimb / Q000294:innervation / Q000601:surgery; D006736:Horses / Q000601:surgery*; D009380:Neoplasms, Nerve Tissue / Q000517:prevention & control; D009416:Nerve Regeneration / Q000187:drug effects*; D009463:Neuroma / Q000517:prevention & control / Q000662:veterinary; D010525:Peripheral Nerves / Q000187:drug effects* / Q000473:pathology / Q000502:physiology / Q000601:surgery; D011182:Postoperative Care / Q000662:veterinary; D014014:Tissue Adhesives / Q000494:pharmacology*
chemical_list: D014014:Tissue Adhesives; D004659:Enbucrilate