Catecholamines, lymphocyte subsets, and cyclic adenosine monophosphate production in mononuclear cells and CD4+ cells in response to submaximal resistance exercise.
We examined the effect of 30 min of submaximal resistance exercise on free and sulphoconjugated plasma catecholamine concentrations determined by high performance (-pressure) liquid chromatography separation, the distribution of circulating lymphocytes quantified by flow cytometry, and isoproterenol induced cyclic adenosine monophosphate (cAMP) production in mononuclear cells (MNL) and CD4+ cells. Venous blood samples were taken before, immediately after and 45 min after exercise. Resistance exercise increased free plasma adrenaline (A) and noradrenaline (NA) concentrations, whereas sulphoconjugated catecholamine concentrations remained unchanged. Exercise induced leucocytosis and lymphocytosis was predominantly manifested by an increase in the number of total lymphocytes, monocytes, CD3+, CD8+ cells and CD3- CD16/CD56+ cells. Redistribution resulted in a decrease in the CD4+:CD8+ ratio. The total number and distribution of lymphocytes returned to baseline after 45-min rest. An exercise-induced increase in the number of CD3- CD16/CD56+ cells was significantly correlated with the increase in plasma NA (r = 0.66; P = 0.035), indicating a NA dependent process of redistribution. The cAMP-production in MNL was significantly elevated after resistance exercise, when cells were stimulated with 1 mumol.1(-1) isoproterenol [pre-exercise 16.5 (SD 3.3); postexercise 21.6 (SD 9.8); 45 min postexercise 10.7 (SD 2.8)]. The cAMP production in CD4+ cells was not affected by exercise. Therefore, it is discussed whether redistribution is responsible for the exercise induced increase in cAMP production in MNL.
Stock
C
C
Institute of Sports Medicine, University-GH-Paderborn, Germany.
Schaller
K
K
Baum
M
M
Liesen
H
H
Weiss
M
M
D000328:Adult; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002395:Catecholamines / Q000097:blood*; D000242:Cyclic AMP / Q000096:biosynthesis*; D006801:Humans; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology; D016131:Lymphocyte Subsets / Q000166:cytology*; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D005082:Physical Exertion
D002395:Catecholamines; D000242:Cyclic AMP