title: FFA-1, a protein that promotes the formation of replication centers within nuclei.
abstract: In the nuclei of eukaryotic cells, initiation of DNA replication occurs at a discrete number of foci. One component of these foci is the DNA replication factor RP-A. Here, the process leading to the association of RP-A with foci was reconstituted with cytosolic fractions derived from Xenopus eggs. With the use of this fractionated system, a 170-kilodalton protein required for the assembly of RP-A into foci was identified and purified. The protein appears to be an integral component of the foci at which replication of DNA is initiated in eukaryotic nuclei.
authors.affiliation: University of California, San Diego, Department of Biology, La Jolla, CA 92093, USA.
authors.forename: H
authors.initials: H
authors.lastname: Yan
authors.forename: J
authors.initials: J
authors.lastname: Newport
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002474:Cell-Free System; D003600:Cytosol / Q000737:chemistry; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005260:Female; D008297:Male; D008970:Molecular Weight; D009865:Oocytes; D009949:Orientation; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D051716:Replication Protein A; D014981:Xenopus
chemical_list: D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D011506:Proteins; D051716:Replication Protein A