Nutrient-enhanced survival of and phenanthrene mineralization by alginate-encapsulated and free Pseudomonas sp. UG14Lr cells in creosote-contaminated soil slurries.
The effects of nutrient amendment and alginate encapsulation on survival of and phenanthrene mineralization by the bioluminescent Pseudomonas sp. UG14Lr in creosote-contaminated soil slurries were examined. UG14Lr was inoculated into creosote-contaminated soil slurries either as a free cell suspension or encapsulated in alginate beads prepared with montmorillonite clay and skim milk. Additional treatments were free-cell-inoculated slurries amended with sterile alginate beads, free-cell-inoculated and uninoculated slurries amended with skim milk only, and uninoculated, unamended slurries. Mineralization was determined by measuring 14CO2 released from radiolabelled phenanthrene. Survival was measured by selective plating and bioluminescence. Inclusion of skim milk was found to enhance both survival of and phenanthrene mineralization by free and encapsulated UG14Lr cells.
Weir
S C
SC
Department of Environmental Biology, University of Guelph, Ontario, Canada.
Dupuis
S P
SP
Providenti
M A
MA
Lee
H
H
Trevors
J T
JT
D000464:Alginates; D000818:Animals; D003407:Creosote; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008163:Luminescent Measurements; D008863:Microspheres; D008892:Milk; D010616:Phenanthrenes / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants; D014169:Transformation, Bacterial
D000464:Alginates; D006603:Hexuronic Acids; D010616:Phenanthrenes; D012989:Soil Pollutants; C031181:phenanthrene; D003407:Creosote; D020723:Glucuronic Acid