Simple and sensitive laser-induced fluorescence detection for capillary electrophoresis and its application to protein separation.
A low-cost and highly sensitive laser-induced fluorescence detector for on-column detection systems was constructed and its applications were demonstrated in protein separation by capillary electrophoresis. The limit of detection (LOD) at a signal-to-noise ratio of 2 for Lissamine 20 was about 1.4 x 10(-21) mole at 10 nL sample injection and the relative standard deviation (RSD) for 1.5 x 10(-11) M of Lissamine 20 solution was about 15%. These values are comparable to or even better than those reported using a similar on-column detection system. This sensitive LIF detection was applied for the study of tryptic digestion of insulin and for urinary protein profiling.
Lim
H B
HB
Department of Chemistry, Dankook University, Seoul, Korea.
Lee
J J
JJ
Lee
K J
KJ
D004586:Electrophoresis / Q000379:methods*; D006801:Humans; D007328:Insulin / Q000032:analysis; D007834:Lasers; D015394:Molecular Structure; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D007328:Insulin; D011506:Proteins