Functional expression of the human CHIP28 water channel in a yeast secretory mutant.
The temperature-sensitive Saccharomyces cerevisiae mutant strain NY17, deficient in the secretory pathway (sec6-4 mutation), is used for the heterologous expression of the human CHIP28 water channel. After a heat-shock, the protein is present in partially purified post-golgi secretory vesicles. Immunodetection and water transport studies, directly made on the vesicles, showed that CHIP28 is highly expressed and active in the yeast membranes.
Laizé
V
V
Département de Biologie Cellulaire et Moléculaire, Service de Biologie Cellulaire, CEA-Saclay, Gif sur Yvette, France.
Rousselet
G
G
Verbavatz
J M
JM
Berthonaud
V
V
Gobin
R
R
Roudier
N
N
Abrami
L
L
Ripoche
P
P
Tacnet
F
F
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D051398:Aquaporin 1; D020346:Aquaporins; D001483:Base Sequence; D001789:Blood Group Antigens; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005614:Freeze Fracturing; D006801:Humans; D007473:Ion Channels / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D009154:Mutation; D009994:Osmolar Concentration; D011994:Recombinant Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013012:Sorbitol / Q000494:pharmacology; D013696:Temperature; D014170:Transformation, Genetic; D014867:Water / Q000378:metabolism
C496020:AQP1 protein, human; D020346:Aquaporins; D001789:Blood Group Antigens; D007473:Ion Channels; D011994:Recombinant Proteins; D014867:Water; D051398:Aquaporin 1; D013012:Sorbitol; D002731:4-Chloromercuribenzenesulfonate