title: Developments in expression cloning.
abstract: Recent years have seen a dramatic expansion in the range of applications of expression cloning techniques. New vectors and detection methods promise to further broaden the applicability of function-based screening approaches to problems in gene discovery. A major theme in the past year has been the introduction of engineered reporter cells that heighten the sensitivity with which clones expressing cDNAs can be identified.
authors.affiliation: Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA.
authors.forename: B
authors.initials: B
authors.lastname: Seed
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D015870:Gene Expression; D008322:Mammals; D011387:Prokaryotic Cells; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D011994:Recombinant Proteins