Fission yeast Rep2 is a putative transcriptional activator subunit for the cell cycle 'start' function of Res2-Cdc10.
In the yeast cell cycle 'start' requires sets of the Cdc10/ SWI family of transcriptional factors which activate the MCB cis elements contained in genes essential for S phase progression. Fission yeast possess two such overlapping systems, Res1-Cdc10 and Res2-Cdc10, both of which act to start the mitotic and meiotic cycles. We have recently isolated rep2+ as a multicopy suppressor of a temperature-sensitive cdc10 mutant which encodes a zinc finger protein. Here we show that the Rep2 zinc finger protein is an essential component of the active Res2-Cdc10 transcriptional regulator complex and likely to play a role in the control of cell cycle 'start'. Our data suggest that Rep2 is a transcriptional activator subunit which interacts with the MCB binding subunit complex formed by Res2 and Cdc10.
Nakashima
N
N
Department of Biochemistry, University of Tokyo, Faculty of Medicine, Japan.
Tanaka
K
K
Sturm
S
S
Okayama
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000378:metabolism* / Q000502:physiology; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D016193:G1 Phase / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics; D005809:Genes, Regulator / Q000235:genetics; D008540:Meiosis / Q000235:genetics; D008938:Mitosis / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000502:physiology; D012568:Schizosaccharomyces / Q000235:genetics / Q000254:growth & development; D029702:Schizosaccharomyces pombe Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013489:Suppression, Genetic; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014157:Transcription Factors; D016335:Zinc Fingers / Q000235:genetics
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C096254:Rep2 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C061178:cdc10 protein, S pombe; C087051:rep1 protein, S pombe; C087021:res2 protein, S pombe; D004247:DNA; D009584:Nitrogen