Cleavage of BCR/ABL mRNA by synthetic ribozymes--effects on the proliferation rate of K562 cells.
Ribozymes are effective tools for the cleavage of target RNAs in a sequence-specific way. In chronic myelogenous leukemia (CML) the reciprocal translocation of chromosomes 9 and 22 results in the formation of the unique BCR/ABL fusion gene which is believed to play a crucial role in the establishment of CML. In order to decrease the BCR/ABL gene product we designed short synthetic ribozymes and analyzed their effects on the proliferation rate of K562 cells. Ribozymes proved to have a higher inhibitory potential as conventional antisense constructs of comparable nucleotide sequence.
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Medizinische Universitätsklinik Freiburg, Abteilung Hämatologie und Onkologie, Freiburg.
D001483:Base Sequence / Q000235:genetics; D002455:Cell Division / Q000235:genetics*; D002460:Cell Line; D002648:Child; D016044:Fusion Proteins, bcr-abl / Q000235:genetics*; D006801:Humans; D007942:Leukemia, Experimental / Q000235:genetics; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D008969:Molecular Sequence Data; D012326:RNA Splicing / Q000235:genetics*; D016337:RNA, Catalytic / Q000138:chemical synthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000502:physiology*
D016337:RNA, Catalytic; D012333:RNA, Messenger; D016044:Fusion Proteins, bcr-abl