Comparison of high-performance liquid chromatography and capillary zone electrophoresis in penciclovir biodegradation kinetic studies.
High-performance liquid chromatography (HPLC) and capillary zone electrophoresis (CZE) were used in biodegradation kinetic studies. This paper describes a rapid penciclovir separation using CZE with detection limits comparable to HPLC. The ionic-strength mediated stacking technique was employed while good resolution was maintained. With a shorter analysis time, comparable detection limits and no organic solvent consumption, CZE is a better method for penciclovir biodegradation studies than conventional reversed-phase HPLC (RP-HPLC).
Hsu
L C
LC
Environmental Research Laboratory, SmithKline Beecham Corporation, King of Prussia, PA 19406, USA.
Constable
D J
DJ
Orvos
D R
DR
Hannah
R E
RE
D000212:Acyclovir / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D000998:Antiviral Agents / Q000493:pharmacokinetics*; D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006147:Guanine; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000998:Antiviral Agents; C053539:penciclovir; D006147:Guanine; D000212:Acyclovir