Matrix attachment regions and transcribed sequences within a long chromosomal continuum containing maize Adh1.
We provide evidence for the location of matrix attachment sites along a contiguous region of 280 kb on maize chromosome 1. We define nine potential loops that vary in length from 6 kb to > 75 kb. The distribution of the different classes of DNA within this continuum with respect to the predicted structural loops reveals an interesting correlation: the long stretches of mixed classes of highly repetitive DNAs are often segregated into topologically sequestered units, whereas low-copy-number DNAs (including the alcohol dehydrogenase1 [adh1] gene) are positioned in separate loops. Contrary to expectations, several classes of highly repeated elements with representatives in this region were found to be transcribed, and some of these exhibited tissue-specific patterns of expression.
Avramova
Z
Z
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
SanMiguel
P
P
Georgieva
E
E
Bennetzen
J L
JL
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D001665:Binding Sites / Q000235:genetics; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D005784:Gene Amplification; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D015530:Nuclear Matrix / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D018749:RNA, Plant / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism
D018744:DNA, Plant; D018749:RNA, Plant; D000426:Alcohol Dehydrogenase