Isolation and characterization of a multienzyme complex containing DNA replicative enzymes from mitochondria of S. cerevisiae. Multienzyme complex from yeast mitochondria.
A 40 S multienzyme complex containing mtDNA polymerase was isolated from mitochondria of S. cerevisiae by density gradient centrifugation and by gel filtration chromatography. Besides DNA polymerase, RNA polymerase, primase, 3'-->5' exonuclease and an ATPase activities were found to be associated with it. The presence of some of these enzymes were confirmed by Western blot. This high molecular weight multienzyme complex containing DNA has most of the attributes of a putative replisome.
Murthy
V
V
Radiation Biology & Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Pasupathy
K
K
D000908:Antibodies, Fungal / Q000276:immunology; D015153:Blotting, Western; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D019915:DNA Primase; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D007425:Intracellular Membranes / Q000201:enzymology; D008565:Membrane Proteins / Q000302:isolation & purification; D008928:Mitochondria / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000302:isolation & purification* / Q000378:metabolism; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D000908:Antibodies, Fungal; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D019915:DNA Primase; D012316:RNA Nucleotidyltransferases; D012321:DNA-Directed RNA Polymerases; D004259:DNA-Directed DNA Polymerase