ICAM-1 expression in hepatocytes following dissociation of cell-to-cell contact in rats.
ICAM-1 was not detected immunohistologically in hepatocytes in normal rats, but detectable in centrilobular degenerative hepatocytes in carbon tetrachloride-intoxicated rats. ICAM-1 expression was observed even in normal hepatocytes following liver perfusion with Hank's balanced salt solution at a flow rate of 4.2 mL/g liver weight/min or with the same solution containing collagenase or EGTA at the physiological flow rate (1.4 mL/g liver weight/min). Such expression was also observed when liver perfusion was performed after pretreatment of rats with cycloheximide. On electron microscopy, ICAM-1 was exclusively stained on hepatocyte plasma membrane that was detached from the plasma membrane of adjacent hepatocytes. ICAM-1 mRNA and ICAM-1 protein were detected in hepatocytes freshly isolated from normal rats. Thus, ICAM-1 expression in degenerative hepatocytes as well as in hepatocytes following liver perfusion can be assumed to result from dissociation of cell-to-cell contact.
Ohno
A
A
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Mochida
S
S
Arai
M
M
Fujiwara
K
K
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism; D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D017364:Collagenases / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D015870:Gene Expression; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D010477:Perfusion; D051381:Rats; D011916:Rats, Inbred F344; D012016:Reference Values
D002614:Chelating Agents; D018799:Intercellular Adhesion Molecule-1; D004533:Egtazic Acid; D017364:Collagenases