title: A novel 16,23-epoxy-5 beta-cholestane glycoside with potent inhibitory activity on proliferation of human peripheral blood lymphocytes from Ornithogalum saundersiae bulbs.
abstract: A novel 16,23-epoxy-5 beta-cholestane triglycoside (1) was isolated from the bulbs of Ornithogalum saundersiae (Liliaceae). The structure was determined by extensive spectroscopic analysis. The conformation of the E-ring part of 1 was studied through molecular mechanics and molecular dynamics calculation methods. Compound 1 potently inhibited proliferation of peripheral blood lymphocytes provided from a chronic renal failure patient without causing any cytotoxicity in the lymphocytes and HL-60 human leukemia cells.
authors.affiliation: School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kuroda
authors.forename: Y
authors.initials: Y
authors.lastname: Mimaki
authors.forename: Y
authors.initials: Y
authors.lastname: Sashida
authors.forename: T
authors.initials: T
authors.lastname: Hirano
authors.forename: K
authors.initials: K
authors.lastname: Oka
authors.forename: A
authors.initials: A
authors.lastname: Dobashi
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002455:Cell Division / Q000187:drug effects; D002776:Cholestanes / Q000494:pharmacology*; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood; D007951:Leukemia, Myeloid / Q000188:drug therapy; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects*; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014407:Tumor Cells, Cultured
chemical_list: C096502:16,23-epoxycholestane triglycoside; D002776:Cholestanes; D006027:Glycosides; D010936:Plant Extracts