Measurement of phosphotyrosine phosphatase activity using the Folin-Ciocalteu phenol reaction.
An assay for the estimation of phosphotyrosine phosphatase using the Folin-Ciocalteu phenol reaction to monitor enzyme activity is presented. The method is based on the property of the substrate phosphotyrosine not to react as a phenol until it is dephosphorylated. The method is sensitive, there is no interference from the use of detergents and it does not rely on special laboratory equipment to distinguish tyrosine from phosphotyrosine.
Gmeiner
B M
BM
Institute of Medical Chemistry, University of Vienna, Austria.
Seelos
C C
CC
D000595:Amino Acid Sequence; D000818:Animals; D002277:Carcinoma; D003110:Colonic Neoplasms; D007202:Indicators and Reagents; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D008982:Molybdenum; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D051381:Rats; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured; D017973:Tungsten Compounds; D014443:Tyrosine / Q000032:analysis
C029556:Folin's phenol reagent; D007202:Indicators and Reagents; D017973:Tungsten Compounds; D014443:Tyrosine; D008982:Molybdenum; D017027:Protein Tyrosine Phosphatases