Sixty new STSs (sequence-tagged sites) of human chromosome 21.
From human chromosome 21-specific libraries, 22 SfiI linking clones and 38 P1 clones were isolated and regionally mapped on the chromosome. The terminal sequences of these clones were determined and pairs of PCR primers were generated which could specifically amplify the sequenced regions. These sequence-tagged sites (STSs) should be useful for constructing a high resolution map of human chromosome 21.
Tanahashi
H
H
Human Genome Center, University of Tokyo, Japan.
Ito
T
T
Hattori
M
M
Ohira
M
M
Ohki
M
M
Tashiro
K
K
Sakaki
Y
Y
D001483:Base Sequence; D002891:Chromosomes, Human, Pair 21; D017931:DNA Primers / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D016324:Sequence Tagged Sites
D017931:DNA Primers; C041749:endodeoxyribonuclease SfiI; D015252:Deoxyribonucleases, Type II Site-Specific