Search for rearrangements and/or allelic loss of the fas/APO-1 gene in 101 human lymphomas.
The authors analyzed the possible occurrence of rearrangements and/or allelic loss of the fas/APO-1 gene in a representative series of human lymphomas, including 101 cases of Hodgkin's disease (HD) and non-Hodgkin's lymphomas (NHL). The rationale for this study was double. Chromosome 10 alterations, frequently observed in lymphoma subtypes, encompass the chromosomal localization of fas/APO-1. In addition, Ipr mouse mutants, which present with a generalized lymphoproliferative disease, were shown to exhibit alterations of fas/APO-1 structure and expression. In this retrospective study, the authors performed gene dosage of fas/APO-1 by Southern blots. No fas/APO-1 alterations were observed in the 31 HD cases. Among 70 T-cell and B-cell NHL, allelic loss of fas/APO-1 was observed in three cases. Two cases with different clinical, phenotypic, and histologic presentations showed a rearrangement of fas/APO-1. A third case showed amplification. Thus fas/APO-1 alterations do occur in human lymphomas, although at a relatively low frequency.
Xerri
L
L
Laboratoire d'Anatomie Pathologique et de Biologie Moléculaire des Tumeurs, Institut Paoli-Calmettes, Marseille, France.
Carbuccia
N
N
Parc
P
P
Birg
F
F
D000483:Alleles; D015139:Blotting, Southern; D005260:Female; D017353:Gene Deletion; D018628:Gene Dosage; D015321:Gene Rearrangement; D005796:Genes; D006689:Hodgkin Disease / Q000235:genetics; D006801:Humans; D008223:Lymphoma / Q000235:genetics*; D016393:Lymphoma, B-Cell / Q000235:genetics; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics; D016399:Lymphoma, T-Cell / Q000235:genetics; D008297:Male; D008875:Middle Aged; D012189:Retrospective Studies; D019014:fas Receptor / Q000235:genetics*
D019014:fas Receptor