Enzymatic properties of lipase and characteristics production by Lactobacillus delbrueckii subsp. bulgaricus.
Some properties of an extracellular lipase produced by Lactobacillus delbrueckii subsp. bulgaricus were studied. Maximum enzyme activity was found against olive and butter oil as enzyme substrates. Addition of 9% acacia gum, 0.1% Na-deoxycholate and 0.01 M CaCl2 to the enzyme reaction mixture increased-lipase activity from 5.3 to 14.5 (FFA/mg protein/minute) at pH 6.0 and at 40 degrees C. Maximum lipase production was reached in the presence of glucose as a sole source of carbon, wheat bran as nitrogen source, olive oil as a sole lipid source and butyric acid as fatty acid supporting the growth medium. An initial pH value of the culture medium of 6.0 and a temperature of 35 degrees C gave the highest lipolytic activity.
el-Sawah
M M
MM
Microbiology Department, Faculty of Agriculture, Mansoura University, Egypt.
Sherief
A A
AA
Bayoumy
S M
SM
D002087:Butyrates; D020148:Butyric Acid; D002122:Calcium Chloride / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000494:pharmacology; D004043:Dietary Fiber; D005947:Glucose; D006170:Gum Arabic / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000187:drug effects / Q000201:enzymology*; D008049:Lipase / Q000096:biosynthesis / Q000378:metabolism*; D000069463:Olive Oil; D010938:Plant Oils; D013379:Substrate Specificity; D013696:Temperature
D002087:Butyrates; D004043:Dietary Fiber; D000069463:Olive Oil; D010938:Plant Oils; D003840:Deoxycholic Acid; D020148:Butyric Acid; D006170:Gum Arabic; D008049:Lipase; D005947:Glucose; D002122:Calcium Chloride