title: Near-patient rapid assay of phenytoin concentration.
abstract: The Biotrack 516 is a simple, automated whole blood phenytoin (PHT) assay that reports corresponding total serum concentrations in 3 min. We compared Biotrack results in 58 patients with the total and unbound serum PHT concentrations measured by the standard TDx fluorescence polarization immunoassay. Correlation with total TDx concentration was high (r = 0.98); median absolute error was 1.4 micrograms/ml. Correlation of unbound PHT with Biotrack (r = 0.95) was comparable to correlation of unbound and total TDx (r = 0.94). The Biotrack assay is a promising method for clinical monitoring of PHT concentrations.
authors.affiliation: Department of Neurology, Yale University School of Medicine, New Haven, Connecticut, USA.
authors.forename: R D
authors.initials: RD
authors.lastname: Scheyer
authors.forename: J M
authors.initials: JM
authors.lastname: Hochholzer
authors.forename: R H
authors.initials: RH
authors.lastname: Mattson
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004912:Erythrocytes / Q000737:chemistry; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000592:standards; D007841:Latex Fixation Tests; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D010672:Phenytoin / Q000097:blood* / Q000627:therapeutic use; D019095:Point-of-Care Systems / Q000592:standards; D011933:Reagent Kits, Diagnostic / Q000592:standards
chemical_list: D011933:Reagent Kits, Diagnostic; D010672:Phenytoin