title: Identification of endogenous protein-associated carbohydrate ligands for E-selectin.
abstract: A comparative analysis of carbohydrate libraries derived from cell lines binding E-selectin was used to identify endogenous protein-associated carbohydrate ligands for E-selectin. Three structures, which together constitute less than 1% of the total cell surface protein-associated carbohydrate, were unique to cell lines capable of binding E-selectin, including neutrophils and the monocytic cell line U937. All are tetra-antennary N-linked structures, with a sialic acid alpha 2 --> 3 galactose beta 1 --> 4 (fucose alpha 1 --> 3) N-acetyl glucosamine beta 1 --> 3 galactose beta 1 --> 4 (fucose alpha 1 --> 3) N-acetyl glucosamine lactosaminoglycan extension (sialyl-di-Lewis X [S-diLe(x)]) on the arm linked through the C4 residue on the mannose. While all contained the expected 3-SLe(x) sub-structure, these native structures have an additional fucosylated lactosamine unit. Direct evidence that these S-di-Lex-containing structures are high-affinity ligands for E-selectin came from the use of recombinant soluble E-selectin-agarose affinity chromatography. These three carbohydrate structures bound specifically to the E-selectin column, while 3-SLe(x) itself does not bind under identical conditions.
authors.affiliation: Oxford GlycoSystems Ltd, Abingdon, Oxon, UK.
authors.forename: T P
authors.initials: TP
authors.lastname: Patel
authors.forename: C J
authors.initials: CJ
authors.lastname: Edge
authors.forename: R B
authors.initials: RB
authors.lastname: Parekh
authors.forename: S E
authors.initials: SE
authors.lastname: Goelz
authors.forename: R R
authors.initials: RR
authors.lastname: Lobb
mesh_terms: D001665:Binding Sites; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry / Q000302:isolation & purification*; D002460:Cell Line; D019040:E-Selectin / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D015394:Molecular Structure; D011485:Protein Binding; D012680:Sensitivity and Specificity
chemical_list: D002241:Carbohydrates; D019040:E-Selectin; D008024:Ligands