title: Sequence of the gene encoding the 16S rRNA of the beer spoilage organism Megasphaera cerevisiae.
abstract: The 16S ribosomal RNA gene from the beer-spoilage organism, Megasphaera cerevisiae was polymerase chain reaction (PCR)-amplified and sequenced. Analysis confirmed the phylogenetic position of M. cerevisiae as a sister taxon of Megasphaera elsdenii, within the obligately anaerobic, Gram-negative cocci. The sequence obtained should facilitate the development of DNA probes for early detection of this spoilage organism.
authors.affiliation: Department of Microbiology, University College, Galway, Ireland.
authors.forename: L M
authors.initials: LM
authors.lastname: Doyle
authors.forename: J O
authors.initials: JO
authors.lastname: McInerney
authors.forename: J
authors.initials: J
authors.lastname: Mooney
authors.forename: R
authors.initials: R
authors.lastname: Powell
authors.forename: A
authors.initials: A
authors.lastname: Haikara
authors.forename: A P
authors.initials: AP
authors.lastname: Moran
mesh_terms: D001483:Base Sequence; D001515:Beer / Q000382:microbiology*; D016964:Gram-Negative Anaerobic Cocci / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S