A novel G protein-coupled receptor mediating both vasopressin- and oxytocin-like functions of Lys-conopressin in Lymnaea stagnalis.
We have cloned a receptor, named LSCPR, for vasopressin-related Lys-conopressin in Lymnaea stagnalis. Lys-conopressin evokes Ca(2+)-dependent Cl- currents in Xenopus oocytes injected with LSCPR cRNA. Expression of LSCPR mRNA was detected in central neurons and peripheral muscles associated with reproduction. Upon application of Lys-conopressin, both neurons and muscle cells depolarize owing to an enhancement of voltage-dependent Ca2+ currents and start firing action potentials. Some neurons coexpress LSCPR and Lys-conopressin, suggesting an autotransmitter-like function for this peptide. Lys-conopressin also induces a depolarizing response in LSCPR-expressing neuroendocrine cells that control carbohydrate metabolism. Thus, in addition to oxytocin-like reproductive functions, LSCPR mediates vasopressin-like metabolic functions of Lys-conopressin as well.
van Kesteren
R E
RE
Department of Experimental Zoology, Graduate School of Neurosciences Amsterdam, Institute of Neuroscience, Vrije Universiteit, Amsterdam, The Netherlands.
Tensen
C P
CP
Smit
A B
AB
van Minnen
J
J
van Soest
P F
PF
Kits
K S
KS
Meyerhof
W
W
Richter
D
D
van Heerikhuizen
H
H
Vreugdenhil
E
E
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002118:Calcium / Q000494:pharmacology; D018118:Chloride Channels / Q000502:physiology; D003001:Cloning, Molecular; D004553:Electric Conductivity; D005260:Female; D019204:GTP-Binding Proteins / Q000502:physiology*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D008195:Lymnaea / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D009928:Organ Specificity; D010121:Oxytocin / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D018045:Receptors, Oxytocin / Q000737:chemistry; D017483:Receptors, Vasopressin / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D016415:Sequence Alignment; D014667:Vasopressins / Q000502:physiology*; D014981:Xenopus
D018118:Chloride Channels; C096468:LSCPR1 protein, Lymnaea stagnalis; D008565:Membrane Proteins; D012333:RNA, Messenger; D018045:Receptors, Oxytocin; D017483:Receptors, Vasopressin; D014667:Vasopressins; C076621:conopressin G; D010121:Oxytocin; D019204:GTP-Binding Proteins; D002118:Calcium