title: Molecular cloning and sequencing of cytochrome c' from the phototrophic purple sulfur bacterium Chromatium vinosum.
abstract: The gene for cytochrome c' from Chromatium vinosum was cloned from a HindIII-SalI digest of genomic DNA. A 1.4 kbp fragment containing the gene was sequenced in both directions using the Sanger dideoxy method. The cytochrome c' gene codes for a 154-residue peptide, of which the last 131 amino acids match the previously determined sequence of the protein. The remaining 23 residues represent a signal sequence that is cleaved from the polypeptide upon translocation to the periplasmic space. An additional open reading frame on the other strand of the fragment codes for a peptide that contains four regions that are homologous to corresponding regions of the cytochrome b-type subunit of several Ni-Fe hydrogenases.
authors.affiliation: Department of Chemistry, University of Illinois at Chicago 60607, USA.
authors.forename: M T
authors.initials: MT
authors.lastname: Even
authors.forename: R J
authors.initials: RJ
authors.lastname: Kassner
authors.forename: M
authors.initials: M
authors.lastname: Dolata
authors.forename: T E
authors.initials: TE
authors.lastname: Meyer
authors.forename: M A
authors.initials: MA
authors.lastname: Cusanovich
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002844:Chromatium / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000737:chemistry / Q000235:genetics*; D004269:DNA, Bacterial; D008969:Molecular Sequence Data
chemical_list: D003574:Cytochrome c Group; D004269:DNA, Bacterial