Production of waxy (amylose-free) wheats.
The Waxy (Wx) protein has been identified as granule-bound starch synthase (GBSS; EC 24.1.21), which is involved in amylose synthesis in plants. Although common wheat (Triticum aestivum L.) has three Wx proteins, "partial waxy mutants" lacking one or two of the three proteins have been found. Using such partial waxy mutants, tetra- and hexaploid waxy mutants with endosperms that are stained red-brown by iodine were produced. Both mutants showed loss of Wx protein and amylose. This is the first demonstration of genetic modification of wheat starch.
Nakamura
T
T
Tohoku National Agricultural Experiment Station, Iwate, Japan.
Yamamori
M
M
Hirano
H
H
Hidaka
S
S
Nagamine
T
T
D004591:Electrophoresis, Polyacrylamide Gel; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics*; D011123:Polyploidy; D013214:Starch Synthase / Q000235:genetics*; D014908:Triticum / Q000235:genetics / Q000502:physiology*
D010940:Plant Proteins; C060756:waxy protein, plant; D013214:Starch Synthase