Detection of two hypervariable (ATTTT)n loci in the human genome.
Object of this investigation was the isolation of a single-locus probe from a multi-locus fingerprint. Individual specific multi-locus fingerprints in man were generated by using the oligonucleotide probe (ATTTT)5. An isolated (ATTTT)5-positive DNA fragment was analyzed using polymerase chain reaction (PCR) cycle-sequencing and nonradioactive direct-blotting electrophoresis. A digoxigenated oligonucleotide synthesized according to this sequence was used as a single-locus probe. Two hypervariable loci were detected on Southern blots. Formal genetic investigations for the two loci were performed in order to estimate the allele frequencies. Locus 1 shows an individual-specific banding pattern with an autosomal-codominant inheritance and can be used for forensic investigations. Locus 2 also represents a polymorphic pattern, but the inheritance is not according to the Mendelian rules. Probably we have detected a highly mutagenic locus in the human genome.
Dürr
C
C
Institut für Anthropologie und Humangenetik, Tübingen, Germany.
Hinney
A
A
Luckenbach
C
C
Ritter
H
H
D001483:Base Sequence; D002648:Child; D016172:DNA Fingerprinting; D005260:Female; D014644:Genetic Variation; D015894:Genome, Human; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes / Q000138:chemical synthesis / Q000235:genetics; D010375:Pedigree; D012091:Repetitive Sequences, Nucleic Acid
D015345:Oligonucleotide Probes