title: Thyroid hormone increases the partitioning of glucose transporters to the plasma membrane in ARL 15 cells.
abstract: The stimulation of glucose transport by 3,5,3'-triiodo-L-thyronine (T3) in the liver-derived ARL 15 cell line is only partly attributable to increased GLUT-1 glucose transporter gene expression. To test the hypothesis that T3 increases the partitioning of GLUT-1 to the cell surface, we quantitated surface GLUT-1 using the photolabel ATB-[3H]BMPA. In control cells only approximately 20% of total cellular GLUT-1 was present at the cell surface. T3 treatment (100 nM) for 6 h increased the rate of 2-deoxy-[3H]glucose (2-DG) uptake by 30, 92, and 95% in three experiments and increased surface GLUT-1 photolabeling by 17, 81, and 72%, respectively, with no increase in total cellular GLUT-1. T3 treatment for 48 h increased 2-DG uptake by 143, 172, and 216% in three experiments and increased cell surface GLUT-1 photolabeling by 88, 161, and 184%, respectively, with smaller increases in total cellular GLUT-1. T3 treatment for 48 h thus increased the fraction of cellular GLUT-1 at the plasma membrane from 21 +/- 2 to 35 +/- 3% (SE). We conclude that most of the early (6-h) stimulation of glucose transport by T3 in ARL 15 cells is mediated by an increase in the partitioning of GLUT-1 to the plasma membrane. With more chronic T3 treatment (48 h), the enhanced surface partitioning of GLUT-1 is persistent and is superimposed on an increase in total cellular GLUT-1, accounting for a further increase in glucose transport.
authors.affiliation: Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA.
authors.forename: R S
authors.initials: RS
authors.lastname: Haber
authors.forename: C M
authors.initials: CM
authors.lastname: Wilson
authors.forename: S P
authors.initials: SP
authors.lastname: Weinstein
authors.forename: A
authors.initials: A
authors.lastname: Pritsker
authors.forename: S W
authors.initials: SW
authors.lastname: Cushman
mesh_terms: D000345:Affinity Labels; D000818:Animals; D001386:Azides; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003847:Deoxyglucose / Q000493:pharmacokinetics; D004187:Disaccharides; D006027:Glycosides; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D011437:Propylamines; D051381:Rats; D013963:Thyroid Hormones / Q000097:blood*; D013997:Time Factors; D014284:Triiodothyronine / Q000494:pharmacology*
chemical_list: D000345:Affinity Labels; D001386:Azides; D004187:Disaccharides; D006027:Glycosides; D009004:Monosaccharide Transport Proteins; D011437:Propylamines; D013963:Thyroid Hormones; D014284:Triiodothyronine; C065412:2-N-(4-(1-azitrifluoroethyl)benzoyl)-1,3-bis-(mannos-4-yloxy)-2-propylamine; D003847:Deoxyglucose