title: Coadsorption of IgG and BSA onto sulfonated polystyrene latex: I. Sequential and competitive coadsorption isotherms.
abstract: In this work the sequential and competitive coadsorption of IgG and BSA proteins on a sulfonate polystyrene latex with high surface charge density have been studied. For sequential coadsorption the IgG/a-CRP was first adsorbed and then the free surface of the particle was saturated by redispersion of the pellet in a solution with a high concentration of monomeric BSA (m-BSA). The competitive coadsorption experiments were carried out in two separate experiments by changing the initial concentration of one protein when the concentration of other protein was high and constant. During the incubation the pH was 5 or 6, and the ionic strength 2 mM, as in previous studies the adsorption of BSA was very low at neutral or basic pH regardless of the amount of adsorbed IgG. From these coadsorption experiments it was possible to obtain latex-protein complexes with a similar degree of coverage by each protein, high adsorption of IgG and different amounts of BSA, or high adsorption of BSA and a low, but significant, amount of IgG. The latex-protein complexes were electrokinetically characterized by measuring the electrophoretic mobility of each complex vs the pH of redispersion. In that way we can detect the i.e.p. of the complexes and the pH range in which the electrostatic repulsion can make them colloidally stable.
authors.affiliation: Department of Applied Physics, University of Granada, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Peula
authors.forename: R
authors.initials: R
authors.lastname: Hidalgo-Alvarez
authors.forename: F J
authors.initials: FJ
authors.lastname: de las Nieves
mesh_terms: D000327:Adsorption; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000378:metabolism*; D007840:Latex; D010316:Particle Size; D011137:Polystyrenes / Q000737:chemistry / Q000378:metabolism*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013451:Sulfonic Acids / Q000737:chemistry
chemical_list: D007074:Immunoglobulin G; D007840:Latex; D011137:Polystyrenes; D013451:Sulfonic Acids; D012710:Serum Albumin, Bovine