Local comparison of the genomes of Mycobacterium tuberculosis and Mycobacterium leprae using the polymerase chain reaction.
To facilitate comparison of the genome maps of Mycobacterium tuberculosis and M. leprae, sequence data from the M. leprae sequencing project were used to design primers suitable for the amplification of short segments from conserved genes by the polymerase chain reaction. In most cases, both organisms yielded products of identical size that were then used as probes for a comparative genomic walk. The hybridization data often, but not always, revealed a similar organization of various regions of both genomes. This approach should be useful for systematic comparisons of (myco)bacterial genomes.
Philipp
W J
WJ
Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France.
Cole
S T
ST
D000595:Amino Acid Sequence; D001483:Base Sequence; D016386:Chromosome Walking; D017931:DNA Primers; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009166:Mycobacterium leprae / Q000235:genetics*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers