title: Increased spontaneous production of IL-8 in peripheral blood monocytes from the psoriatic patient: relation to focal infection and response to treatments.
abstract: To evaluate the contribution of peripheral blood monocytes (PBMC) to epidermotropic inflammatory reactions in psoriasis, using an enzyme-linked immunosorbent assay we measured spontaneous interleukin-8 (IL-8) production in PBMC obtained from patients with psoriasis. IL-8 production in the psoriatic PBMC was significantly higher than that in normal control PBMC. Plasma IL-8 levels in psoriatic patients were also moderately increased compared to normal control levels. IL-8 production in PBMC was closely related to the clinical severity of psoriasis and to the response to treatment, including systemic methotrexate (MTX) treatment and tonsillectomy. IL-8 production in PBMC was also positively related to the production of interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) in these cells. We speculate that the chemotactic cytokine IL-8 contributes to the development of psoriatic skin lesions, and mediates inflammatory reactions from the inflammatory focus to the psoriatic lesions.
authors.affiliation: Department of Dermatology, Mie University, Faculty of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Teranishi
authors.forename: H
authors.initials: H
authors.lastname: Mizutani
authors.forename: M
authors.initials: M
authors.lastname: Murata
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
authors.forename: K
authors.initials: K
authors.lastname: Matsushima
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000097:blood*; D008297:Male; D008727:Methotrexate / Q000627:therapeutic use; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D014066:Palatine Tonsil / Q000503:physiopathology; D010812:Physical Stimulation; D011565:Psoriasis / Q000097:blood* / Q000628:therapy*; D012720:Severity of Illness Index; D013997:Time Factors; D014068:Tonsillectomy
chemical_list: D016209:Interleukin-8; D008727:Methotrexate