title: Methionine-393 is an axial ligand of the heme b558 component of the cytochrome bd ubiquinol oxidase from Escherichia coli.
abstract: The cytochrome bd oxidase is one of two terminal oxidases in the aerobic respiratory chain of Escherichia coli. The complex is composed of two subunits (I and II) and three heme prosthetic groups (heme b558, heme b595, and a chlorin, called heme d). Both subunits are located within the bacterial cytoplasmic membrane, and each has multiple putative transmembrane helices. Heme b558 is a six-coordinate, low-spin heme component of the oxidase which has been shown to be contained within subunit I and has been implicated in the oxidation of the substrate, ubiquinol-8, in the cytoplasmic membrane. Previous site-directed mutagenesis studies identified His186, predicted to be near the periplasmic side of transmembrane helix D of subunit I, as one of the axial ligands of heme b558. Since mutagenesis of none of the other histidines in subunit I perturbs heme b558, it was concluded that this heme cannot have bis(histidine) ligation. In this work, the properties of 14 mutants are reported, including substitutions for each of 10 methionine residues within subunit I. Among this set of mutants, only the replacement of M393 perturbs heme b558. Replacement of M393 by leucine results in the conversion of heme b558 to a high-spin state. Surprisingly, the M393L mutation does not eliminate enzymatic activity, and the mutant oxidase has sufficient turnover to support aerobic growth of the cells. The addition of imidazole to the purified M393L oxidase converts heme b558 back to a low-spin configuration. The data strongly suggest that the sixth axial ligand of heme b558 is methionine-393, and that this heme, therefore, has histidine-methionine ligation. The results are consistent with recent cryogenic near-infrared magnetic circular dichroism spectra that also indicate histidine-methionine ligation of heme b558.
authors.affiliation: School of Chemical Sciences, University of Illinois, Urbana 61801, USA.
authors.forename: T M
authors.initials: TM
authors.lastname: Kaysser
authors.forename: J B
authors.initials: JB
authors.lastname: Ghaim
authors.forename: C
authors.initials: C
authors.lastname: Georgiou
authors.forename: R B
authors.initials: RB
authors.lastname: Gennis
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003573:Cytochrome b Group / Q000737:chemistry* / Q000378:metabolism*; D003580:Cytochromes / Q000737:chemistry* / Q000378:metabolism*; D004227:Dithionite / Q000494:pharmacology; D045222:Electron Transport Chain Complex Proteins; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005838:Genotype; D006418:Heme / Q000378:metabolism*; D006639:Histidine; D007930:Leucine; D008024:Ligands; D046911:Macromolecular Substances; D008715:Methionine; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D019255:NADPH Oxidases; D009838:Oligodeoxyribonucleotides; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D017354:Point Mutation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015183:Restriction Mapping; D013053:Spectrophotometry
chemical_list: D003573:Cytochrome b Group; D003580:Cytochromes; D045222:Electron Transport Chain Complex Proteins; D029968:Escherichia coli Proteins; D008024:Ligands; D046911:Macromolecular Substances; D009838:Oligodeoxyribonucleotides; D011994:Recombinant Proteins; D004227:Dithionite; D006418:Heme; D006639:Histidine; C036888:cytochrome b558; D008715:Methionine; D010088:Oxidoreductases; D019255:NADPH Oxidases; C040077:cytochrome bd terminal oxidase complex, E coli; D007930:Leucine