title: Anti-CD4 activity of normal human immunoglobulin G for therapeutic use. (Intravenous immunoglobulin, IVIg).
abstract: The effects of intravenously administered normal immunoglobulin G (IVIg) in autoimmune diseases are dependent on the ability of IVIg to interact with surface molecules of lymphocytes. In the present study, we demonstrate the presence of anti-CD4 activity in IVIg by showing the ability of IVIg to bind to CD4 and to inhibit CD4-dependent cellular functions. Binding of IVIg to recombinant soluble human CD4 was assessed by ELISA, immunoblotting and real time analysis of complex formation. Anti-CD4 antibodies isolated from IVIg by affinity-chromatography bound to human CD4+ T cells. These anti-CD4 antibodies inhibited proliferative responses in MLR and infection of CD4+ human T cells with HIV. These results indicate that IVIg contains antibodies reactive with human CD4 and that these anti-CD4 antibodies exhibit biological functions. The presence of anti-CD4 antibodies in IVIg may be relevant to the immunoregulatory effects of normal polyspecific immunoglobulin G.
authors.affiliation: INSERM U 28, Hôpital Broussais, Paris, France.
authors.forename: V
authors.initials: V
authors.lastname: Hurez
authors.forename: S V
authors.initials: SV
authors.lastname: Kaveri
authors.forename: A
authors.initials: A
authors.lastname: Mouhoub
authors.forename: G
authors.initials: G
authors.lastname: Dietrich
authors.forename: J C
authors.initials: JC
authors.lastname: Mani
authors.forename: D
authors.initials: D
authors.lastname: Klatzmann
authors.forename: M D
authors.initials: MD
authors.lastname: Kazatchkine
mesh_terms: D000276:Adjuvants, Immunologic; D015153:Blotting, Western; D015704:CD4 Antigens / Q000276:immunology*; D019085:Fluorescent Antibody Technique, Direct; D015658:HIV Infections / Q000276:immunology; D015497:HIV-1 / Q000254:growth & development; D006801:Humans; D007118:Immunoassay; D016756:Immunoglobulins, Intravenous / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D011485:Protein Binding
chemical_list: D000276:Adjuvants, Immunologic; D015704:CD4 Antigens; D016756:Immunoglobulins, Intravenous