Porcine SINE-associated microsatellite markers: evidence for new artiodactyl SINEs.
Approximately 24% (170/710) of porcine (dG-dT)n.(dC-dA)n microsatellites isolated in our laboratory are associated with a previously described porcine Short Interdispersed Element (SINE) termed PRE-1 SINE. Another 5.6% (40/710) of the microsatellites were adjacent to two previously unidentified SINE sequences, which we have designated ARE-1P (Artiodactyl Repetitive Element-1 Porcine) and ARE-2P. The ARE repeats were also found in bovine microsatellite and genomic sequences in the GenBank database. Genotypic information was obtained from 68.9% of primers where at least one primer sequence was obtained from the PRE-1 SINE and 66.6% of primer pairs designed from the ARE SINEs. The use of primers derived from SINEs significantly increases the number of primer pairs available for genetic linkage studies in swine.
Alexander
L J
LJ
United States Department of Agriculture, ARS, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 68933, USA.
Rohrer
G A
GA
Stone
R T
RT
Beattie
C W
CW
D000818:Animals; D001188:Artiodactyla / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle; D016384:Consensus Sequence; D017931:DNA Primers; D005838:Genotype; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D012756:Sheep; D013552:Swine / Q000235:genetics*
D017931:DNA Primers