title: Emergence in C kappa knockout mice of a diverse cytotoxic T lymphocyte repertoire that recognizes a single peptide from the immunoglobulin constant kappa light chain region.
abstract: Allotype- or idiotype-specific CD4+ T cells have been reported to recognize immunoglobulin (Ig) peptides presented by class II molecules. In contrast, few data are available concerning the generation of Ig peptide-specific CD8+ T cells. We have therefore investigated whether T-depleted spleen cells from Ig kappa light chain-expressing 129/Sv mice (129 kappa +/+) could induce, in C kappa knockout mice (129 kappa -/-), the generation of Ig constant kappa light chain region (C kappa)-specific cytotoxic T lymphocytes (CTL). The determination of TCR beta chain expressed by nine CTL clones, together with the use of a library of overlapping peptides spanning the whole C kappa sequence, show that the B cells from kappa +/+ mice are able to elicit in C kappa knockout mice, the emergence of a diverse CTL repertoire that recognizes one single C kappa peptide presented by the H-2Kb class I molecule. In addition, these data support the notion that B cells are able to process and present on their class I molecules, peptides generated from their own kappa light chains.
authors.affiliation: Département d'Immunologie, Institut Pasteur (URA CNRS 1961 and Université Pierre et Marie Curie), Paris, France.
authors.forename: D
authors.initials: D
authors.lastname: Rueff-Juy
authors.forename: P
authors.initials: P
authors.lastname: Sanchez
authors.forename: M
authors.initials: M
authors.lastname: Faure
authors.forename: A M
authors.initials: AM
authors.lastname: Drapier
authors.forename: P A
authors.initials: PA
authors.lastname: Cazenave
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D017690:Cell Transplantation; D003602:Cytotoxicity, Immunologic; D000939:Epitopes / Q000276:immunology; D005803:Genes, Immunoglobulin; D006183:H-2 Antigens / Q000276:immunology; D007127:Immunoglobulin Constant Regions / Q000235:genetics / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000235:genetics / Q000276:immunology*; D008223:Lymphoma / Q000473:pathology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D051381:Rats; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D013154:Spleen / Q000637:transplantation; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014407:Tumor Cells, Cultured
chemical_list: D000939:Epitopes; D006183:H-2 Antigens; D007127:Immunoglobulin Constant Regions; D007145:Immunoglobulin kappa-Chains; D010446:Peptide Fragments; D016693:Receptors, Antigen, T-Cell, alpha-beta