Construction of a recombinant cellulolytic Escherichia coli.
A 1.2-kb DNA fragment from Bacillus subtilis CD4 encoding endo-beta-1,4-glucanase and cellobiase activities was cloned and expressed in Escherichia coli. Carboxymethylcellulase (CMCase) and cellobiase activities were detected in a cell-free lysate of recombinant (re-) E. coli (Ec). The re-enzymes were functional in Ec, as it utilized carboxymethylcellulose, soluble cellulose and cellobiose as sole carbon sources for growth.
Srivastava
R
R
Division of Microbial Genetics, Central Drug Research Institute, Lucknow, India.
Kumar
G P
GP
Srivastava
K K
KK
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics; D002482:Cellulose / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005818:Genetic Engineering; D006026:Glycoside Hydrolases / Q000235:genetics; D011994:Recombinant Proteins / Q000378:metabolism
D011994:Recombinant Proteins; D002482:Cellulose; D006026:Glycoside Hydrolases