title: Cloning, subcellular localization and expression of CHL1, a subunit of magnesium-chelatase in soybean.
abstract: Mg-insertion is the first committed step in chlorophyll synthesis and is catalyzed by Mg-chelatase. In photosynthetic bacteria, bchI gene product was suggested to be a subunit of Mg-chelatase. We isolated a bchI homolog from a soybean cDNA library and designated it as chlI. CHLI consisted of 421 amino acid residues and the sequence exhibited a high similarity to other BchI homologs. CHLI contained an ATP-binding motif found in other BchI homologs. CHLI was localized in the soluble fraction in soybean chloroplasts, suggesting that it was a stromal subunit of Mg-chelatase. chlI mRNA in cell culture (SB-P) of soybean was reversibly induced by light.
authors.affiliation: Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nakayama
authors.forename: T
authors.initials: T
authors.lastname: Masuda
authors.forename: N
authors.initials: N
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Yamagata
authors.forename: C
authors.initials: C
authors.lastname: Bowler
authors.forename: H
authors.initials: H
authors.lastname: Ohta
authors.forename: Y
authors.initials: Y
authors.lastname: Shioi
authors.forename: K
authors.initials: K
authors.lastname: Takamiya
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology; D001665:Binding Sites; D015152:Blotting, Northern; D015153:Blotting, Western; D002736:Chloroplasts / Q000201:enzymology; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000737:chemistry; D005056:Euglena gracilis / Q000201:enzymology; D015870:Gene Expression; D008190:Lyases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000378:metabolism; D016354:Rhodobacter capsulatus / Q000201:enzymology; D016415:Sequence Alignment; D013025:Glycine max / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology*
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D000255:Adenosine Triphosphate; D008190:Lyases; C086788:magnesium chelatase