title: Molecular and phylogenetic analysis of PCR-amplified cyclin-dependent kinase (CDK) family sequences from representatives of the earliest available lineages of eukaryotes.
abstract: Cyclin-dependent kinase (CDK) and cell division control (CDC2) sequences are strongly conserved among eukaryotes and may complement the use of other sequence families in eukaryotic phylogenetic inference. We synthesized degenerate PCR primers to amplify the catalytic region of CDK homologs in representatives of the earliest available lineages of eukaryotes. CDK family sequence-based, maximum-likelihood distance measurements with neighbor-joining, and Fitch-Margoliash least-squares analyses produced unrooted dendrograms that included protists, yeasts, and higher eukaryotes. Bootstrap confidence estimates supported CDK-based phylogenetic groupings among the protists, fungi, and vertebrates although resolution within these groups was often insignificant. However, Trichomonas vaginalis and Giardia lamblia exhibited two of the most divergent CDK-like sequences consistent with rRNA-phylogenetic inference of early divergence of these eukaryotic lineages. In the evolution from unicellular to multicellular organisms, a constellation of amino acid residues aligning with the human, CDK N-terminal beta sheet may have undergone abrupt replacement.
authors.affiliation: Department of Urology, School of Medicine, University of Washington, Seattle 98195, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Riley
authors.forename: J N
authors.initials: JN
authors.lastname: Krieger
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D016001:Confidence Intervals; D018844:Cyclin-Dependent Kinases / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D056890:Eukaryota / Q000235:genetics; D005057:Eukaryotic Cells / Q000201:enzymology; D005658:Fungi / Q000235:genetics; D014644:Genetic Variation; D016829:Giardia lamblia / Q000201:enzymology / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D017386:Sequence Homology, Amino Acid; D014246:Trichomonas vaginalis / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D014714:Vertebrates / Q000235:genetics
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan; D018844:Cyclin-Dependent Kinases