title: Odor responses after complete desensitization of the cAMP-dependent pathway in turtle olfactory cells.
abstract: The degree of contribution of the cAMP signal transduction pathway to odor responses was examined by recording current responses from isolated turtle olfactory cells under the whole-cell voltage clamp conditions. The cAMP signal transduction pathway was desensitized by dialyzing 1 mM cAMP and 0.5 mM IBMX from the patch pipette into the cells. Extracellular application of 3 mM cpt-cAMP, a membrane-permeable cAMP analogue elicited no response, indicating that the cAMP pathway was completely desensitized. Application of an odorant cocktail induced a large inward current under these conditions, suggesting that the cAMP-independent signal transduction contributes significantly to generation of odor responses in the turtle.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kashiwayanagi
authors.forename: K
authors.initials: K
authors.lastname: Kurihara
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000818:Animals; D000242:Cyclic AMP / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D009812:Odorants; D018408:Patch-Clamp Techniques; D015398:Signal Transduction / Q000187:drug effects*; D012903:Smell / Q000187:drug effects*; D013268:Stimulation, Chemical; D014426:Turtles
chemical_list: D000242:Cyclic AMP; D015056:1-Methyl-3-isobutylxanthine