title: Perturbations in DNA structure upon interaction with porphyrins revealed by chemical probes, DNA footprinting and molecular modelling.
abstract: The interactions of several porphyrins with a 74 base-pair DNA sequence have been examined by footprinting and chemical protection methods. Tetra-(4-N-methyl-(pyridyl)) porphyrin (TMPy), two of its metal complexes and tetra-(4-trimethylanilinium) porphyrin (TMAP) bind to closely similar AT-rich sequences. The three TMPy ligands produce modest changes in DNA structure and base accessibility on binding, in contrast to the large-scale conformational changes observed with TMAP. Molecular modelling studies have been performed on TMPy and TMAP bound in the AT-rich minor groove of an oligonucleotide. These have shown that significant structural change is needed to accommodate the bulky trimethyl substituent groups of TMAP, in contrast to the facile minor groove fit of TMPy.
authors.affiliation: CRC Biomolecular Structure Unit, Institute of Cancer Research, Sutton, Surrey, U.K.
authors.forename: K G
authors.initials: KG
authors.lastname: Ford
authors.forename: S
authors.initials: S
authors.lastname: Neidle
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D018983:DNA Footprinting; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D011166:Porphyrins / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D011166:Porphyrins; C021096:tetra(4-N-methylpyridyl)porphine; D004247:DNA