title: Xanthine oxidase inactivation attenuates postocclusion shock after descending thoracic aorta occlusion and reperfusion in rabbits.
abstract: "Declamping shock" is observed after aortic crossclamping, with hypovolemia, hypotension, and metabolic acidemia invariably present. We hypothesized that oxidants derived from xanthine oxidase influence the resuscitative interventions required to maintain baseline hemodynamic and acid-base status after aortic occlusion and reperfusion in rabbits. We also hypothesized that inactivation of xanthine oxidase with sodium tungstate could reduce systemic injury as assessed by the release of lactate dehydrogenase and alkaline phosphatase. To test these hypotheses, we established aortic occlusion in rabbits (n = 10, standard diet; n = 8, tungstate diet) for 40 minutes by inflation of a 4F Fogarty catheter in the descending thoracic aorta followed by 2 hours of reperfusion. Sham-operated rabbits (n = 10, standard diet; n = 9, tungstate diet) served as controls. Tungstate-pretreated rabbits required significantly less Ringer's solution (28%), phenylephrine (68%), and sodium bicarbonate (30%) during reperfusion (p < 0.005). Lactate dehydrogenase and alkaline phosphatase release during reperfusion was significantly attenuated by tungstate pretreatment (p < 0.05). Tungstate pretreatment resulted in plasma xanthine oxidase activities significantly lower than those in the sham group administered a standard diet (p = 0.007). Resuscitation requirements and systemic injury were reduced by inactivation of xanthine oxidase in a rabbit model that simulates the situation of human thoracic aorta operations.
authors.affiliation: Department of Anesthesiology, University of Alabama at Birmingham 35233-6810, USA.
authors.forename: V G
authors.initials: VG
authors.lastname: Nielsen
authors.forename: A T
authors.initials: AT
authors.lastname: McCammon
authors.forename: S
authors.initials: S
authors.lastname: Tan
authors.forename: K A
authors.initials: KA
authors.lastname: Kirk
authors.forename: P N
authors.initials: PN
authors.lastname: Samuelson
authors.forename: D A
authors.initials: DA
authors.lastname: Parks
mesh_terms: D000136:Acid-Base Equilibrium; D000469:Alkaline Phosphatase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001013:Aorta, Thoracic; D003250:Constriction; D004789:Enzyme Activation / Q000187:drug effects; D006439:Hemodynamics; D007552:Isotonic Solutions / Q000008:administration & dosage; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D010656:Phenylephrine / Q000008:administration & dosage; D011817:Rabbits; D015427:Reperfusion Injury / Q000150:complications / Q000503:physiopathology / Q000517:prevention & control*; D012151:Resuscitation; D000077331:Ringer's Solution; D012773:Shock, Surgical / Q000209:etiology / Q000503:physiopathology / Q000517:prevention & control*; D017693:Sodium Bicarbonate / Q000008:administration & dosage; D017973:Tungsten Compounds / Q000494:pharmacology; D014969:Xanthine Oxidase / Q000097:blood / Q000378:metabolism*
chemical_list: D007552:Isotonic Solutions; D017973:Tungsten Compounds; D010656:Phenylephrine; C025399:sodium tungstate(VI); D000077331:Ringer's Solution; D017693:Sodium Bicarbonate; D007770:L-Lactate Dehydrogenase; D014969:Xanthine Oxidase; D000469:Alkaline Phosphatase