Cloning and chromosomal localization of a pseudogene corresponding to a mRNA for a soluble IL-6 receptor.
A polymerase chain reaction assay (Lust J.A. et al. (1992). Cytokine 4:96-100) was used to detect a mRNA coding for a soluble IL-6 receptor in human hepatoma cells. In addition to the expected amplification product, we found a sequence (SR4) which could be aligned to it with 78% identity. After cloning and sequencing a genomic 2.5-2.7 kB EcoRI fragment containing SR4, this sequence turned out to be part of a pseudogene corresponding to the transmembrane domain deleted soluble IL-6 receptor. Screening of a panel of interspecies hybrids revealed that it maps to chromosome 9.
Snyers
L
L
Institut Pasteur du Brabant, Bruxelles, Belgium.
Renauld
J C
JC
Szpirer
C
C
Content
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J
D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D017931:DNA Primers; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011544:Pseudogenes; D012333:RNA, Messenger / Q000737:chemistry / Q000302:isolation & purification*; D018123:Receptors, Interleukin / Q000235:genetics*; D019947:Receptors, Interleukin-6; D016415:Sequence Alignment; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D017931:DNA Primers; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D019947:Receptors, Interleukin-6