Rapid purification of recombinant antibody fragments for catalysis screening.
Huang
H
H
Department of Anesthesiology, University of Nebraska Medical Center, Omaha, USA.
Fichter
B
B
Dannenbring
R
R
Paul
S
S
D002384:Catalysis; D002845:Chromatography / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli; D006639:Histidine; D006801:Humans; D006868:Hydrolysis; D007128:Immunoglobulin Fragments / Q000302:isolation & purification* / Q000378:metabolism*; D007147:Immunoglobulin Light Chains / Q000302:isolation & purification / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D011994:Recombinant Proteins / Q000302:isolation & purification* / Q000378:metabolism; D014660:Vasoactive Intestinal Peptide / Q000276:immunology / Q000378:metabolism*
D007128:Immunoglobulin Fragments; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D011994:Recombinant Proteins; D014660:Vasoactive Intestinal Peptide; D006639:Histidine