title: Lasp-1 (MLN 50) defines a new LIM protein subfamily characterized by the association of LIM and SH3 domains.
abstract: MLN 50 was previously identified in a cDNA library of breast cancer metastasis. In this study, we show that MLN 50, which is expressed at a basal level in normal tissues, is overexpressed in 8% of human breast carcinomas most often together with c-erbB-2. MLN 50 cDNA encodes a putative protein of 261 residues, named Lasp-1 (LIM and SH3 protein) since it contains a LIM motif and a domain of Src homology region 3 (SH3) at the amino- and the C-terminal parts of the protein, respectively. Thus, Lasp-1 defines a new LIM protein subfamily.
authors.affiliation: Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/U184 INSERM/ULP, Illkirch, France.
authors.forename: C
authors.initials: C
authors.lastname: Tomasetto
authors.forename: C
authors.initials: C
authors.lastname: Moog-Lutz
authors.forename: C H
authors.initials: CH
authors.lastname: Régnier
authors.forename: V
authors.initials: V
authors.lastname: Schreiber
authors.forename: P
authors.initials: P
authors.lastname: Basset
authors.forename: M C
authors.initials: MC
authors.lastname: Rio
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D001943:Breast Neoplasms / Q000378:metabolism*; D002886:Chromosomes, Human, Pair 17; D016384:Consensus Sequence / Q000235:genetics; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D060588:LIM Domain Proteins; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins; D011494:Protein Kinases / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018909:src Homology Domains
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D018398:Homeodomain Proteins; D060588:LIM Domain Proteins; D009363:Neoplasm Proteins; D011494:Protein Kinases; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; C096353:LASP1 protein, human