title: Sequence analysis of leukotoxin secretion determinants from a Pasteurella haemolytica-like organism.
abstract: The pHLBD genes encoding the secretion functions for the 105 kDa RTX leukotoxin of Pasteurella haemolytica-like (PHL) organism has been cloned and sequenced. Like analogous genes from other RTX determinants, the pHLBD genes lie immediately downstream from the leukotoxin structural gene, pHLA. Although isolated from a diverse group of gram-negative organisms, the pHLBD genes and the characterized RTX BD genes from other organisms exhibit a high degree of homology at both the DNA and predicted amino acid sequence levels. We have previously reported the cloning of the leukotoxin gene (pHLCA) (Chang et al., Infect. Immun. 61:2089-2095), which encodes a 105-kda polypeptide with cytotoxic activity. DNA sequence analysis of the pHLBD genes shows 83.93% and 86.05% homologous to that of P. haemolytica IktBD genes, respectively.
authors.affiliation: Diagnostic Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Y F
authors.initials: YF
authors.lastname: Chang
authors.forename: D P
authors.initials: DP
authors.lastname: Ma
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: N
authors.initials: N
authors.lastname: Chin
authors.forename: M M
authors.initials: MM
authors.lastname: Chengappa
authors.forename: S J
authors.initials: SJ
authors.lastname: Shin
authors.forename: D H
authors.initials: DH
authors.lastname: Lein
authors.forename: H J
authors.initials: HJ
authors.lastname: Yoon
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000378:metabolism; D001483:Base Sequence; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000235:genetics; D005098:Exotoxins / Q000378:metabolism*; D005798:Genes, Bacterial; D006092:Gram-Negative Facultatively Anaerobic Rods / Q000235:genetics; D016978:Mannheimia haemolytica / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000382:microbiology
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D002352:Carrier Proteins; D005098:Exotoxins; C096182:PhlB protein, Serratia liquefaciens; C096183:PhlD protein, Pseudomonas; C018579:leukotoxin