PCR-based characterization of Yersinia enterocolitica: comparison with biotyping and serotyping.
PCR-based DNA fingerprinting was used to characterize 48 clinical isolates of Yersinia enterocolitica. The samples were examined by random amplified polymorphic DNA (RAPD-PCR) and inter-repeat PCR (IR-PCR). IR-PCR with two enterobacterial repetitive intergenic consensus primers resulted in patterns which were poorly discriminated; 2 of 11 arbitrary primers (RAPD-PCR) provided sufficient discriminatory power. In comparisons with serotyping and biotyping, RAPD-fingerprinting was the most discriminatory technique and may therefore be a valuable epidemiological tool for the study of Y. enterocolitica infections.
Odinot
P T
PT
University Hospital Nijmegen, Department of Medical Microbiology, The Netherlands.
Meis
J F
JF
Van den Hurk
P J
PJ
Hoogkamp-Korstanje
J A
JA
Melchers
W J
WJ
D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D004751:Enteritis / Q000453:epidemiology / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D009426:Netherlands / Q000453:epidemiology; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D012703:Serotyping / Q000379:methods*; D015009:Yersinia Infections / Q000453:epidemiology / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000145:classification / Q000235:genetics*
D004269:DNA, Bacterial