title: High-performance capillary electrophoretic separation of proteins and peptides using a bonded hydrophilic phase capillary.
abstract: High performance capillary electrophoresis (HPCE) was applied to the separation of protein and peptide mixtures with molecular masses ranging from 1300 to 96000 Da using a new bonded hydrophilic phase capillary, CElect-P150. This coated capillary reduces the interaction between proteins and silanol groups in capillary walls, allowing a complete recovery of the proteins and peptides of interest. HPCE was also used for the analysis of a complex mixture of tryptic fragments and to monitor the process of enzymatic digestion. Moreover, using a CElect-P150 capillary, highly reproducible analysis was possible without preconditioning the capillary with acid or basic solutions before each new analysis.
authors.affiliation: Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Piccoli
authors.forename: M
authors.initials: M
authors.lastname: Fiorani
authors.forename: B
authors.initials: B
authors.lastname: Biagiarelli
authors.forename: F
authors.initials: F
authors.lastname: Palma
authors.forename: L
authors.initials: L
authors.lastname: Vallorani
authors.forename: R
authors.initials: R
authors.lastname: De Bellis
authors.forename: V
authors.initials: V
authors.lastname: Stocchi
mesh_terms: D000818:Animals; D002417:Cattle; D003574:Cytochrome c Group / Q000737:chemistry / Q000378:metabolism; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D014357:Trypsin
chemical_list: D003574:Cytochrome c Group; D010455:Peptides; D011506:Proteins; D012710:Serum Albumin, Bovine; D014357:Trypsin