title: Characterization of a rat oral squamous cell carcinoma cell line UHG-RaC '93 induced by 4-nitroquinoline-1-oxide in vivo.
abstract: This study describes several characteristics of a cell line, UHG-RaC '93 derived from rat oral squamous cell carcinoma induced by the carcinogen 4-nitroquinoline-1-oxide (4NQO). The cell line was established from explant cultures without support of fibroblast feeder cells and continued for > 30 passages. UHG-RaC '93 had a high mitotic rate with a population doubling time of 25 h and a high rate of squame production. The first passage had a low colony-forming efficiency in agarose gel, whereas later passages did not grow at all in semi-solid medium. Phenotype selection was furthermore apparent from a gradual increase of the trypsin-detachment time. Cytogenetic analysis showed that UHG-RaC '93 was hypotetraploid with an average of 74 chromosomes. Abnormalities compared to the normal karyotype were assessed and consisted mainly of breakpoints at (1)(q5?3), (3)(p1), (3)(q11q23), (11)(p?11), (13)(p13) and a derivative (12)t(12;13)(q10;q10). The karyotype remained stable for at least 26 passages. The expression of typical epithelioid markers like cytokeratins and desmoglein corresponded with normal rat oral keratinocytes. However expression of alpha 6 beta 4-integrin was altered. Squame production, immunophenotype and anchorage dependency indicated that UHG-RaC '93 had the same features of a well-differentiated carcinoma with a low degree of agressiveness as the original tumour. The stable karyotype of this cell line provides a basis for further analysis of the effect of 4NQO on the genotype, phenotype and behaviour of rat oral keratinocytes.
authors.affiliation: Department of Oral and Maxillofacial Surgery, University Hospital Groningen, The Netherlands.
authors.forename: M
authors.initials: M
authors.lastname: Witjes
authors.forename: J
authors.initials: J
authors.lastname: Scholma
authors.forename: E
authors.initials: E
authors.lastname: van Drunen
authors.forename: J L
authors.initials: JL
authors.lastname: Roodenburg
authors.forename: G
authors.initials: G
authors.lastname: Mesander
authors.forename: A
authors.initials: A
authors.lastname: Hagemeijer
authors.forename: A M
authors.initials: AM
authors.lastname: Tomson
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000235:genetics / Q000473:pathology*; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D005455:Fluorescent Antibody Technique; D016023:Integrins / Q000032:analysis; D008297:Male; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000235:genetics / Q000473:pathology*; D009363:Neoplasm Proteins / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured
chemical_list: D002273:Carcinogens; D016023:Integrins; D009363:Neoplasm Proteins; D015112:4-Nitroquinoline-1-oxide