Expression of the cyclin-dependent kinase inhibitor p16 during the ongoing cell cycle.
It has been demonstrated that protein expression of p16, the inhibitor of cyclin-dependent kinase 4 and 6, increases 4 fold at the G1/S transition when serum-arrested cells are restimulated to logarithmic growth. We examined the cell cycle regulation of this cyclin-dependent kinase inhibitor in cells separated according to their cell cycle phases by centrifugal elutriation. Neither p16 mRNA nor its protein expression are regulated during the cell cycle of normal phytohemagglutinin-stimulated lymphocytes, retinoblastoma protein-negative cells, papilloma virus-transformed cells, and acute promyelocytic leukemia cells. p16 mRNA is constitutively expressed in cells in which we detected the normal E2F-dependent S-phase specific expression of thymidine kinase mRNA. We further observed a G1-phase specific expression of cyclin D1 mRNA in the same cells separated by centrifugal elutriation.
Soucek
T
T
Department of Prenatal Diagnosis and Therapy, University of Vienna, Austria.
Pusch
O
O
Hengstschläger-Ottnad
E
E
Wawra
E
E
Bernaschek
G
G
Hengstschläger
M
M
D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis / Q000096:biosynthesis*; D002453:Cell Cycle; D002455:Cell Division; D019938:Cyclin D1; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016213:Cyclins / Q000096:biosynthesis; D004791:Enzyme Inhibitors / Q000032:analysis; D005134:Eye Neoplasms; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D015513:Oncogene Proteins / Q000096:biosynthesis; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D012175:Retinoblastoma; D016160:Retinoblastoma Protein / Q000032:analysis / Q000096:biosynthesis; D013937:Thymidine Kinase / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D002352:Carrier Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016213:Cyclins; D004791:Enzyme Inhibitors; D015513:Oncogene Proteins; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D016160:Retinoblastoma Protein; D019938:Cyclin D1; D013937:Thymidine Kinase