Effect of s-triazine compounds on testosterone metabolism in the rat prostate.
The influence of s-triazine compounds (atrazine, prometryne and deethylatrazine) on testosterone conversion and 5 alpha-dihydrotestosterone-receptor complex formation was studied in vitro and in vivo in the rat prostate. A marked in vitro influence of atrazine and prometryne (from 0.465 to 1.392 mumol) and their mixtures (in total concentration, 0.928 mumol) on 5 alpha-dihydrotestosterone formation was detected. 5 alpha-Dihydrotestosterone-specific receptor complex formation was inhibited in vitro by ca. 0.5 mumol of atrazine or deethylatrazine and only in vivo by 6 mg of atrazine 100 g-1 body wt. daily during 7 days in the prostate cytosol. The inhibition of the enzymic activities responsible for testosterone conversion and steroid hormone-receptor complex formation was non-competitive and reversible, and s-triazine compounds act as antiandrogens.
Kniewald
J
J
Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia.
Osredecki
V
V
Gojmerac
T
T
Zechner
V
V
Kniewald
Z
Z
D000730:Androstadienes / Q000378:metabolism; D015113:Androstane-3,17-diol / Q000378:metabolism; D000818:Animals; D001280:Atrazine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity*; D001665:Binding Sites; D003600:Cytosol / Q000187:drug effects / Q000473:pathology; D005043:Etiocholanolone / Q000031:analogs & derivatives / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D011400:Prometryne / Q000008:administration & dosage / Q000633:toxicity*; D011467:Prostate / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344; D011944:Receptors, Androgen / Q000187:drug effects / Q000378:metabolism*; D013237:Stereoisomerism; D013739:Testosterone / Q000378:metabolism*
D000730:Androstadienes; D011944:Receptors, Androgen; D011400:Prometryne; C006573:1,4-androstadiene-3,17-dione; D015113:Androstane-3,17-diol; D013739:Testosterone; C006576:androstane-3,17-dione; C027879:4-deethylatrazine; D005043:Etiocholanolone; D001280:Atrazine