Effect of inhibitors on the transport of dinitrophenyl-S-glutathione in human erythrocytes.
Effect of inhibitors on and pH dependence of the export of dinitrophenyl-S-glutathione (DNP-SG), a glutathione S-conjugate formed upon in vivo conjugation of 1-chloro-2,4-dinitrobenzene to glutathione, was studied in intact human erythrocytes. The transport was inhibited by orthovanadate (IC50 = 80 microM) and fluoride (IC50 = 9 nM). Erythrocyte anion exchange (Band 3) protein inhibitors DIDS and SITS did not exert any significant effect on the transport. The transport rate increased with increasing extracellular pH in the range of 6.0-8.0. The Arrhenius activation energy of the process was 72.4 +/- 1.3 kJ/mol. Membrane potential, extracellular sodium/potassium concentration ratio and extracellular osmolality in the range 300-600 mOsm did not influence the export rate.
Pułaski
L
L
Department of Molecular Biophysics, University of Lódź, Poland.
Bartosz
G
G
D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology; D002352:Carrier Proteins / Q000037:antagonists & inhibitors*; D004912:Erythrocytes / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D005978:Glutathione / Q000031:analogs & derivatives* / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D026901:Membrane Transport Proteins; D014638:Vanadates / Q000494:pharmacology
D002352:Carrier Proteins; D026901:Membrane Transport Proteins; C086860:glutathione transporter; C030190:S-(2,4-dinitrophenyl)glutathione; D014638:Vanadates; D005978:Glutathione; D005033:Ethylmaleimide; D005459:Fluorides; D002118:Calcium