Immunoreactivity of PKC gammalambda and RACK1 in baker's yeast, lobster and wheat germ.
Varied patterns of immunoreactive bands of protein kinase C gamma (PKC gamma) and receptor for activated C-kinase-1 (RACK1) were detected by analysis of Western blots in crude extracts of wheat germ, lobster tail meat, and three strains of baker's yeast. Anti-PKC lambda also reacted with wheat germ and yeast extracts, but failed to react with the lobster extract. The findings may implicate a regulatory role and an evolutionary conservation of these PKC isoenzymes and their receptor proteins in eukaryotes.
Kuo
W N
WN
Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, Florida 32114-3099, USA.
Jones
D L
DL
Ku
T W
TW
Weeks
K D
KD
Jordon
P M
PM
Dopson
N C
NC
D000818:Animals; D002457:Cell Extracts; D006367:HeLa Cells; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D008121:Nephropidae / Q000737:chemistry; D010455:Peptides / Q000032:analysis*; D011493:Protein Kinase C / Q000032:analysis*; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000737:chemistry; D014908:Triticum / Q000737:chemistry; D000099293:Protein Kinase C-lambda
D002457:Cell Extracts; D007527:Isoenzymes; D010455:Peptides; D011493:Protein Kinase C; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D000099293:Protein Kinase C-lambda; C070301:peptide I; C087857:protein kinase C gamma