The use of genomic DNA probes for in-gel hybridization.
Hybridization within agarose gels using oligonucleotide probes has been described in several publications; genomic DNA probes, however, have been used rarely and only with limited success. Here we present a simple and convenient procedure for in-gel hybridization using radiolabeled genomic DNA fragments. The protocol was improved by the use of formamide in the hybridization as well as in the washing step. This method was compared with the conventional Southern blotting technique and was shown to produce good results in restriction pattern analysis, as well as in chromosomal localization with the help of pulsed field gel electrophoresis.
Wöhl
T
T
Max-Planck-Institut für Biochemie, Martinsried, Germany.
Brecht
M
M
Lottspeich
F
F
Ammer
H
H
D015139:Blotting, Southern; D002874:Chromosome Mapping; D015342:DNA Probes; D004271:DNA, Fungal / Q000235:genetics; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005057:Eukaryotic Cells; D005782:Gels; D005800:Genes, Fungal; D007553:Isotope Labeling; D009693:Nucleic Acid Hybridization; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D015342:DNA Probes; D004271:DNA, Fungal; D005782:Gels