title: Oral fluid as a specimen for detection and confirmation of antibodies to human immunodeficiency virus type 1.
abstract: Paired serum and oral fluid specimens (n = 287) were collected with the Omni-Sal device and were assayed for the presence of antibodies to human immunodeficiency virus type 1 (HIV-1). Enzyme immunoassays (EIAs)--Abbott 3A11, an Organon Teknika Corporation research-use-only test, and the Murex GACELISA--were used per the manufacturers' inserts or were modified slightly to accommodate the oral fluid specimens. Compared with serum Western blot (immunoblot) results, each EIA had a sensitivity of 100% and the specificities were 89.6% for the Abbott 3A11 EIA, 96.5% for the GACELISA, and 97.8% for the Organon Teknika Corporation EIA. Specificities based on specimens that were repeatedly reactive were 99.3% for all EIAs. A miniaturized Western blot technique used for confirmatory testing of both the serum and oral fluid specimens found 149 of the 287 samples to be HIV-1 antibody positive in both sample types. The Western blot banding patterns observed for the serum and oral fluid specimens were essentially identical. Immunoglobulin G concentrations were determined for all oral fluid specimens and ranged from < 0.5 to > 40.0 micrograms/ml. Immunoglobulin G concentrations did not correlate with the ability of any of the EIAs to detect HIV-1-specific antibody or with the ability of the modified Western blot to detect HIV-1 protein-specific antibodies.
authors.affiliation: Division of HIV/AIDS, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
authors.forename: T C
authors.initials: TC
authors.lastname: Granade
authors.forename: S K
authors.initials: SK
authors.lastname: Phillips
authors.forename: B
authors.initials: B
authors.lastname: Parekh
authors.forename: C P
authors.initials: CP
authors.lastname: Pau
authors.forename: J R
authors.initials: JR
authors.lastname: George
mesh_terms: D000293:Adolescent; D000328:Adult; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015483:HIV Antibodies / Q000032:analysis* / Q000097:blood; D015658:HIV Infections / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008875:Middle Aged; D012463:Saliva / Q000276:immunology*
chemical_list: D015483:HIV Antibodies; D007074:Immunoglobulin G