title: Molecular genetic evidence for unifocal origin of advanced epithelial ovarian cancer and for minor clonal divergence.
abstract: Detection of loss of heterozygosity (LOH) and DNA flow cytometry (FCM) were used to trace the origin of bilateral ovarian cancer from 16 patients. From each tumour the DNA index (DI) and LOH patterns for chromosomes 1, 3, 6, 11, 17, 18, 22 and X were determined with 36 microsatellite markers. Formalin-fixed, paraffin-embedded as well as frozen specimens were used. Flow cytometric cell sorting was used to enrich tumour cells for polymerase chain reaction (PCR)-driven LOH analysis. Analysis of the LOH data showed that in 12 of the 16 cases concordance was observed for all informative markers, namely retention of heterozygosity (ROH) or loss of identical alleles in both tumour samples. In four cases discordant LOH patterns were observed. In two cases the discordant LOH was found for one of the chromosomes tested while other LOH patterns clearly indicated a unifocal origin. This suggests limited clonal divergence. In the other two cases all LOH patterns were discordant, most likely indicating an independent origin. The number of chromosomes showing LOH ranged from 0 to 6. Comparison of DNA FCM and the LOH data showed that the latter technique has a higher sensitivity for the detection of a unifocal origin. In 14/16 cases evidence was found for a unifocal origin, while in two cases clonal divergence was found at LOH level and in two other cases clonal divergence at DNA ploidy level. In 12 cases the complete observed allelotype had developed before the formation of metastases, including the two cases showing a large DNA ploidy difference.
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Leiden, The Netherlands.
authors.forename: E C
authors.initials: EC
authors.lastname: Abeln
authors.forename: N J
authors.initials: NJ
authors.lastname: Kuipers-Dijkshoorn
authors.forename: E M
authors.initials: EM
authors.lastname: Berns
authors.forename: S C
authors.initials: SC
authors.lastname: Henzen-Logmans
authors.forename: G J
authors.initials: GJ
authors.lastname: Fleuren
authors.forename: C J
authors.initials: CJ
authors.lastname: Cornelisse
mesh_terms: D002277:Carcinoma / Q000235:genetics / Q000473:pathology / Q000556:secondary*; D002999:Clone Cells; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000032:analysis; D004848:Epithelium / Q000737:chemistry / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D005629:Frozen Sections; D017353:Gene Deletion; D005819:Genetic Markers; D006801:Humans; D018895:Microsatellite Repeats; D009362:Neoplasm Metastasis; D009378:Neoplasms, Multiple Primary / Q000235:genetics / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology / Q000556:secondary*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction
chemical_list: D004273:DNA, Neoplasm; D005819:Genetic Markers