title: Synthetic antibody gene libraries for in vitro affinity maturation.
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario.
authors.forename: S J
authors.initials: SJ
authors.lastname: Deng
authors.forename: C R
authors.initials: CR
authors.lastname: MacKenzie
authors.forename: S A
authors.initials: SA
authors.lastname: Narang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000915:Antibody Affinity; D001483:Base Sequence; D017931:DNA Primers; D004926:Escherichia coli; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis* / Q000737:chemistry; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis* / Q000737:chemistry; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis* / Q000737:chemistry; D007136:Immunoglobulins / Q000096:biosynthesis*; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D016296:Mutagenesis; D019081:O Antigens / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins / Q000096:biosynthesis*; D015183:Restriction Mapping; D012475:Salmonella / Q000276:immunology
chemical_list: D017931:DNA Primers; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins; D007202:Indicators and Reagents; D019081:O Antigens; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D011994:Recombinant Proteins