Effective blotting of ultrathin polyacrylamide gels anchored to a solid matrix.
Ultrathin polyacrylamide gels bound on glass plates or plastic sheets cannot be removed from their support without destruction. Therefore electrophoretic transfer methods are not applicable. We have developed a fast diffusion blotting procedure which is very simple and does not need any equipment like blotting chamber or power supply. Furthermore, no special buffer solutions are required. The method is universally applicable to ultrathin sodium dodecyl sulfate, native as well as isoelectric focusing polyacrylamide gels.
Heukeshoven
J
J
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Germany.
Dernick
R
R
D000180:Acrylic Resins; D015153:Blotting, Western / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007525:Isoelectric Focusing / Q000379:methods; D011506:Proteins / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry
D000180:Acrylic Resins; D011506:Proteins; C016680:polyacrylamide gels; D012967:Sodium Dodecyl Sulfate