[Antigenic structure of the Bordetella pertussis pertactin Pro-rich segment 536-566. I. Preparation and analysis of antisera to synthetic peptides].
Rabbit antibodies to four synthetic peptides from the Pro-rich sequence of B. pertussis P.69 have been obtained. Prior to immunization, peptides KAPPAPKPAPQPGPQPP (17P), QPPQPQPEAPAPQP (14P), GPQPPQPPQP (10P) and PGPQPP (6P) were conjugated with keyhole limpet hemocyanin (KLH). Immunoenzymatic analysis revealed the following antibody titers: 1:4,000,000 (17P), 1:64,000 (14P) and 1:256,000 (10P). Serum antibodies to KLH-6P did not react with 6P-coated plates but interacted with the hexapeptide in the ovalbumin-6P conjugate. The sera to KLH-14P effectively recognized glutaraldehyde-formed epitopes. Using indirect immunoassay and competitive assay, it was shown that antisera to KLH-17P, KLH-14P, KLH-10P and KLH-6P did not react with unrelated antigens. Sera and affinity-purified antibodies to peptides 10P, 14P and 17P recognized native pertactin with variable efficiency.
Kavun
E M
EM
Borisova
E G
EG
Makarov
E A
EA
Radavskiĭ
Iu L
IuL
D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D001886:Bordetella pertussis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006433:Hemocyanins / Q000276:immunology; D007106:Immune Sera; D018796:Immunoconjugates; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D010566:Virulence Factors, Bordetella
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007106:Immune Sera; D018796:Immunoconjugates; D010446:Peptide Fragments; D010566:Virulence Factors, Bordetella; C068929:pertactin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin
Antigennoe stroenie Pro-bogatogo uchastka 536-566 pertaktina Bordetellapertussis. I. Poluchenie i analiz antisyvorotok k sinteticheskim peptidam.