Aspirin inhibits collagen-induced platelet serotonin release, as measured by microbore high-performance liquid chromatography with electrochemical detection.
A sensitive microbore high-performance liquid chromatographic method with electrochemical detection has been developed for the measurement of small quantities of serotonin (5-hydroxytryptamine; 5-HT) and its metabolite 5-hydroxyindoleacetic acid (5-HIAA) in rabbit platelets. The limit of detection of 5-HT is 0.1 ng/ml. To evaluate the 5-HT release from platelet suspension, the aggregating agent collagen was added at concentrations of 10, 30 and 100 micrograms/ml and the 5-HT concentrations rose from the base level (11.80 +/- 0.42 ng/ml) to 123.97 +/- 11.02, 361.96 +/- 17.90 and 470.45 +/- 35.46 ng/ml, respectively. Further results demonstrated that aspirin inhibits collagen-induced 5-HT release from platelet suspension significantly.
Tsai
T H
TH
National Research Institute of Chinese Medicine, Taipei, Taiwan.
Tsai
W J
WJ
Chen
C F
CF
D000818:Animals; D001241:Aspirin / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003094:Collagen / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D004563:Electrochemistry; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011817:Rabbits; D015203:Reproducibility of Results; D012701:Serotonin / Q000378:metabolism*
D010975:Platelet Aggregation Inhibitors; D012701:Serotonin; D003094:Collagen; D001241:Aspirin