title: Biological characterization and molecular cloning of murine C-type retroviruses derived from the TSZ complex from mainland China.
abstract: Characterization of the SRS murine retrovirus complex, derived from the TSZ system of murine leukemia developed in China, was carried out. The initial stock contained XC+, NB-tropic virus (and possibly other viruses), and induced several neoplastic diseases in neonatally inoculated NIH Swiss mice: erythroid leukemia, myeloid leukemia (acute myeloblastic leukemia), and lymphoblastic lymphoma (both B- and T-lymphoid). In addition, approximately 30% of inoculated animals developed central nervous system disease--hindlimb paralysis or semilateral paralysis. Rescue of virus from the spleen of an animal with combined erythroid/myeloid leukemia, followed by endpoint dilution gave two stocks: 19-6 (XC+, NB-tropic) and 19-7 (XC-, NB-tropic). Both stocks induced erythroid and myeloid leukemia, and 19-6 induced CNS symptoms as well. Southern blot analysis indicated that the predominant viruses from the 19-6 and the 19-7 cultures were related, but different in the env region. An infectious virus molecular clone of provirus from 19-6 cells was obtained. The resulting cloned virus [SRS 19-6 murine leukemia virus (MuLV)] induced four kinds of leukemia: erythroid, myeloid, B-lymphoma, and T-lymphoma; in many cases, more than one tumor type was identified in the same animal. Such a broad spectrum of leukemias induced by a cloned MuLV is unusual. Flaccid hindlimb paralysis induced by SRS 19-6 MuLV could be attributed to meningeal B-lymphoma. Immunofluorescent staining with a panel of env-specific monoclonal antibodies confirmed that the 19-6 and 19-7 viral stocks contained different viruses, which differed from previously characterized MuLVs. The viruses of the SRS complex may provide interesting reagents for investigations of MuLV-induced disease.
authors.affiliation: Department of Molecular Biology and Biochemistry, University of California at Irvine 92717, USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Bundy
authors.forename: M
authors.initials: M
authors.lastname: Ru
authors.forename: B F
authors.initials: BF
authors.lastname: Zheng
authors.forename: L
authors.initials: L
authors.lastname: Cheng
authors.forename: P K
authors.initials: PK
authors.lastname: Pattengale
authors.forename: J L
authors.initials: JL
authors.lastname: Portis
authors.forename: H
authors.initials: H
authors.lastname: Fan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002681:China; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000235:genetics; D015752:Genes, env / Q000235:genetics; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000472:pathogenicity; D007942:Leukemia, Experimental / Q000473:pathology / Q000821:virology*; D051379:Mice; D008969:Molecular Sequence Data; D010243:Paralysis / Q000821:virology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000473:pathology / Q000821:virology; D011533:Proviruses / Q000235:genetics; D015183:Restriction Mapping; D012192:Retroviridae Infections / Q000473:pathology / Q000821:virology*; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000821:virology; D014412:Tumor Virus Infections / Q000473:pathology / Q000821:virology*
chemical_list: D004279:DNA, Viral