An immunodominant antigen of Brugia malayi is an asparaginyl-tRNA synthetase.
Lymphatic filariasis is caused by infection with the filarial nematodes Brugia malayi, Brugia timori, Wuchereria bancrofti and Onchocerca volvulus which collectively infect about 200 million persons throughout the world. Protein sequence homology analysis of a major nematode antigen suggested that it was a class II aminoacyl-tRNA synthetase. The overproduction, purification and verification that the major B. malayi antigen is an asparaginyl-tRNA synthetase is described.
Kron
M
M
Department of Medicine, Michigan State University, East Lansing 48824, USA.
Marquard
K
K
Härtlein
M
M
Price
S
S
Leberman
R
R
D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000947:Antigens, Helminth / Q000235:genetics / Q000276:immunology*; D001226:Aspartate-tRNA Ligase; D001483:Base Sequence; D017178:Brugia malayi / Q000276:immunology*; D017931:DNA Primers; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D012346:RNA, Transfer, Amino Acyl; D012348:RNA, Transfer, Asn; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology
D000947:Antigens, Helminth; D017931:DNA Primers; D016056:Immunodominant Epitopes; D012346:RNA, Transfer, Amino Acyl; D012348:RNA, Transfer, Asn; D011993:Recombinant Fusion Proteins; D000604:Amino Acyl-tRNA Synthetases; D001226:Aspartate-tRNA Ligase; C029194:asparaginyl-tRNA synthetase