Streptavidin-reactive protein in Fasciola hepatica.
Streptavidin-binding protein was found in an extract of Fasciola hepatica, and the protein was analyzed by the inhibition assay using avidin or D-biotin, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting using peroxidase-conjugated streptavidin. Inhibition assays revealed that the protein could bind to streptavidin by a mechanism similar to the avidin-biotin reaction, and the molecular size of the protein was estimated by SDS-PAGE to be 71.7 kDa.
Takeyoshi
M
M
Hita Research Laboratories, Chemicals Inspection and Testing Institute, Oita, Japan.
Inoue
T
T
Iwata
H
H
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis; D000947:Antigens, Helminth / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay / Q000706:statistics & numerical data; D005189:False Positive Reactions; D005210:Fasciola hepatica / Q000276:immunology / Q000378:metabolism*; D015801:Helminth Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D007073:Immunoglobulin E / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011485:Protein Binding; D012680:Sensitivity and Specificity; D019809:Streptavidin
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D001426:Bacterial Proteins; D002352:Carrier Proteins; D015801:Helminth Proteins; D007073:Immunoglobulin E; D001710:Biotin; D019809:Streptavidin