Comparison of Pseudomonas pseudomallei from humans, animals, soil and water by restriction endonuclease analysis.
Pseudomonas pseudomallei isolates from 62 human, 17 animal, 3 soil and 3 water samples were examined by genomic DNA digestion with PstI. Five major (RE I, II, III, IV, V) reproducible restriction patterns were observed, with most (56/62) of the human isolates displaying RE I (30/62), II (5/62), III (15/62), IV (4/62), V (2/62), and the animal (16/17), soil (2/3), water (3/3) isolates showing predominantly RE II profiles. Six human and one soil isolates showed patterns different from those of RE I to V. Restriction endonuclease analysis may be applied in epidemiological studies of melioidosis.
Yap
E H
EH
Department of Microbiology, National University of Singapore.
Thong
T W
TW
Tan
A L
AL
Yeo
M
M
Tan
H C
HC
Loh
H
H
Teo
T P
TP
Thong
K T
KT
Singh
M
M
Chan
Y C
YC
D000818:Animals; D016957:Burkholderia pseudomallei / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D008554:Melioidosis / Q000453:epidemiology; D015183:Restriction Mapping; D012703:Serotyping; D012846:Singapore / Q000453:epidemiology; D012988:Soil Microbiology; D014871:Water Microbiology