Stimulation of ionotropic glutamate receptors activates transcription factor NF-kappa B in primary neurons.
L-Glutamate is the most common excitatory neurotransmitter in the brain and plays a crucial role in neuronal plasticity as well as in neurotoxicity. While a large body of literature describes the induction of immediate-early genes, including c-fos, fosB, c-jun, junB, zif/268, and krox genes by glutamate and agonists in neurons, very little is known about preexisting transcription factors controlling the induction of such genes. This prompted us to investigate whether stimulation of glutamate receptors can activate NF-kappa B, which is present in neurons in either inducible or constitutive form. Here we report that brief treatments with kainate or high potassium strongly activated NF-kappa B in granule cells from rat cerebellum. This was detected at the single cell level by immunostaining with a monoclonal antibody that selectively reacts with the transcriptionally active, nuclear form of NF-kappa B p65. The activation of NF-kappa B could be blocked with the antioxidant pyrrolidine dithiocarbamate, suggesting the involvement of reactive oxygen intermediates. The data may explain the kainate-induced cell surface expression of major histocompatibility complex class I molecules, which are encoded by genes known to be controlled by NF-kappa B. Moreover, NF-kappa B activity was found to change dramatically in neurons during development of the cerebellum between days 5 and 7 after birth.
Kaltschmidt
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C
Institute of Biochemistry, Albert-Ludwigs-University, Freiburg, Germany.
Kaltschmidt
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B
Baeuerle
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PA
D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000494:pharmacology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D003594:Cytoplasmic Granules; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D005805:Genes, MHC Class I; D007150:Immunohistochemistry; D007608:Kainic Acid / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D011759:Pyrrolidines / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017470:Receptors, Glutamate / Q000378:metabolism*; D013859:Thiocarbamates / Q000494:pharmacology; D014158:Transcription, Genetic
D000975:Antioxidants; D018690:Excitatory Amino Acid Agonists; D016328:NF-kappa B; D011759:Pyrrolidines; D017470:Receptors, Glutamate; D013859:Thiocarbamates; C020972:pyrrolidine dithiocarbamic acid; D011188:Potassium; D007608:Kainic Acid