title: Rapid PCR site-directed mutagenesis.
authors.affiliation: Stratagene Cloning Systems, La Jolla, California 92037, USA.
authors.forename: M P
authors.initials: MP
authors.lastname: Weiner
authors.forename: G L
authors.initials: GL
authors.lastname: Costa
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004262:DNA Restriction Enzymes; D004259:DNA-Directed DNA Polymerase; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D014176:Protein Biosynthesis; D016364:Reading Frames; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D015183:Restriction Mapping; D019914:Taq Polymerase
chemical_list: D017931:DNA Primers; D007202:Indicators and Reagents; D011994:Recombinant Proteins; C072146:Pfu DNA polymerase; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes; C020102:endodeoxyribonuclease DpnI; D015252:Deoxyribonucleases, Type II Site-Specific