Human estrogen receptor messenger RNA variants in both normal and tumor breast tissues.
By using the PCR-SSCP technique we characterized various ER-specific RNA species present in a series of primary breast cancers, as well as in cell lines established from breast carcinomas and in mammary gland tissues from healthy specimens. A series of six truncated messenger RNAs generated by alternative splicing was characterized. These RNAs correspond to specific deletions of one (exons 2-7, except exon 6) or two (exons 3 + 4) exons. All these RNA variants are observed in each one of the analyzed RNAs, regardless of origin. In addition, the relative amount of these different variants in ER + tumors is comparable to that measured in ER - tumors and healthy mammary gland tissues. This data suggests that tumor progression is not related to the emergence of any of the ER mRNA variants.
Gotteland
M
M
CNRS, IRSC, Laboratory of Molecular Oncology, Villejuif, France.
Desauty
G
G
Delarue
J C
JC
Liu
L
L
May
E
E
D017398:Alternative Splicing; D001483:Base Sequence; D001940:Breast / Q000737:chemistry*; D001943:Breast Neoplasms / Q000235:genetics*; D018076:DNA, Complementary; D004587:Electrophoresis, Agar Gel; D005091:Exons; D005260:Female; D017353:Gene Deletion; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase; D011960:Receptors, Estrogen / Q000235:genetics*; D013698:Templates, Genetic; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D012194:RNA-Directed DNA Polymerase