title: Peptide ligation and semisynthesis.
abstract: Semisynthesis is used to create defined analogues of proteins by the chemical manipulation of peptide fragments largely derived from the natural protein and the subsequent reassembly of those fragments into a near-native conformation. In common with the total synthesis of proteins, it requires efficient and non-destructive methods for peptide religation. Recently, a wide range of chemoselective ligation schemes have been elaborated that now permit the assembly of minimally protected peptides from either synthetic or natural sources.
authors.affiliation: Dalhousie University, Halifax, Canada.
authors.forename: C J
authors.initials: CJ
authors.lastname: Wallace
mesh_terms: D010446:Peptide Fragments / Q000378:metabolism*; D014176:Protein Biosynthesis; D011487:Protein Conformation; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D013816:Thermodynamics
chemical_list: D010446:Peptide Fragments; D011506:Proteins