Establishment of a cell line from an adenocarcinoma of the lung producing carcinoembryonic antigen (CEA) and CA19-9.
An adenocarcinoma cell line was established from pleural effusion of a 67-year-old woman and designated as TK-SA. The population doubling time of the cells was 100.4 hr. Chromosomal analysis revealed the TK-SA to have human aneuploid chromosomes with a near-triploid mode. Ultrastructurally, the TK-SA was compatible with adenocarcinoma. The cell line was highly tumorigenic. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide dye assay demonstrated resistance of the cell line to Cis-platin (CDDP), Cyclophosphamide (CPM) and Tegafur/Uracil (UFT). High carcinoembryonic antigen (CEA) and CA19-9 levels were similar in patient's serum and conditioned medium.
Miki
M
M
Department of Respiratory Medicine, Tohoku University, Sendai, Japan.
Isawa
T
T
Teshima
T
T
Anazawa
Y
Y
Mahmud
A M
AM
Nukiwa
T
T
D000230:Adenocarcinoma / Q000648:ultrastructure*; D000368:Aged; D000941:Antigens; D002272:Carcinoembryonic Antigen; D002452:Cell Count; D002460:Cell Line; D002875:Chromosomes; D003520:Cyclophosphamide / Q000494:pharmacology; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000648:ultrastructure*; D008854:Microscopy, Electron; D014407:Tumor Cells, Cultured
D000941:Antigens; D002272:Carcinoembryonic Antigen; D003520:Cyclophosphamide