Development of a simple and selective separation of 67Cu from irradiated zinc for use in antibody labelling: a comparison of methods.
A procedure for the production and separation of Cu isotopes from irradiated Zn was developed. Following a comparison of methods based on extraction, electrolysis and ion-exchange chromatography, a technique for the separation of Cu employing three ion-exchange matrices was developed which was simple, reproducible and hot cell-compatible. The specific activity of the final product was 37 MBq 67Cu/microgram Cu at EOB. The level of impurities was so low that no interference with antibody labelling was observed.
Schwarzbach
R
R
Radiopharmacy Division, Paul Scherrer Institute, Würenlingen, Switzerland.
Zimmermann
K
K
Bläuenstein
P
P
Smith
A
A
Schubiger
P A
PA
D000906:Antibodies / Q000737:chemistry*; D002623:Chemistry Techniques, Analytical; D002852:Chromatography, Ion Exchange; D003301:Copper Radioisotopes / Q000302:isolation & purification*; D004572:Electrolysis; D005069:Evaluation Studies as Topic; D007553:Isotope Labeling / Q000379:methods; D011854:Radiochemistry; D015032:Zinc / Q000737:chemistry / Q000528:radiation effects*
D000906:Antibodies; D003301:Copper Radioisotopes; D015032:Zinc