title: Gene fusion expression systems in Escherichia coli.
abstract: In recent years, Escherichia coli gene fusion expression systems have circumvented many of the problems inherent in the use of this bacterium for the production of recombinant proteins. These systems also provide a powerful means for identifying peptides or proteins with desired binding specificities. Gene fusion technology continues to expand with the introduction of new fusion partners, purification and detection tags, cleavage reagents and ways to display peptides on the surface of bacteria.
authors.affiliation: Department of Molecular Biology and Gene Expression, Genetics Institute, Cambridge, Massachusetts 02140, USA.
authors.forename: E R
authors.initials: ER
authors.lastname: LaVallie
authors.forename: J M
authors.initials: JM
authors.lastname: McCoy
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D011993:Recombinant Fusion Proteins