Noradrenaline effects on cultured rat sensory neurones exposed to morphine in utero or in vitro.
Dorsal root ganglion (DRG) neurones cultured from newborn rats were chronically exposed to 1 microM morphine. Untreated cells and in vitro morphine treated cells were cultured for 1 or 2 weeks from control offspring while in utero morphine treated cells were cultured for 1 week from offspring of morphine dependent rats. Noradrenaline (1 microM) was applied by pressure ejection. Intracellular recordings measured the change in action potential duration (APD). Noradrenaline significantly decreased the APD of 1-week-old untreated cells while the 2-week-old untreated cells and the groups of morphine treated cells showed no significant change of APD following the drug application. Thus, newborn rat DRG neurones demonstrated a different sensitivity to noradrenaline with the culture duration or with morphine exposure.
Villière
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Prince of Wales Medical Research Institute, Randwick, NSW, Australia.
Neering
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IR
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured / Q000187:drug effects; D005260:Female; D005727:Ganglia, Spinal / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology*; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors; D014599:Uterus / Q000187:drug effects*
D009020:Morphine; D009638:Norepinephrine