Mouse Col18a1 is expressed in a tissue-specific manner as three alternative variants and is localized in basement membrane zones.
We have isolated overlapping cDNAs encoding the N-terminal non-triple-helical region of mouse alpha 1(XVIII) collagen and shown that three different variants of alpha 1(XVIII) collagen exist. Each of the three variants shows characteristic tissue-specific expression patterns. Immunohistochemical studies show positive staining for alpha 1(XVIII) collagen along the basement membrane zones of vessels in the intestinal villi, the choroid plexus, skin, liver, and kidney. Thus, we conclude that alpha 1(XVIII) collagen may interact (directly or indirectly) with components in basement membrane zones or on the basal surface of endothelial/epithelial cells.
Muragaki
Y
Y
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.
Timmons
S
S
Griffith
C M
CM
Oh
S P
SP
Fadel
B
B
Quertermous
T
T
Olsen
B R
BR
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001485:Basement Membrane / Q000737:chemistry*; D001808:Blood Vessels / Q000737:chemistry; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D018076:DNA, Complementary / Q000235:genetics; D004622:Embryo, Mammalian / Q000737:chemistry / Q000166:cytology; D014644:Genetic Variation; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D014018:Tissue Distribution
D018076:DNA, Complementary; D003094:Collagen