Identification of a stable complex of trichosanthin with nicotinamide adenine dinucleotide phosphate.
Trichosanthin, a type I ribosome-inactivating protein with RNA N-glycosidase activity, forms a stable complex with nicotinamide adenine dinucleotide phosphate, a substrate analog. Difference UV spectroscopy, fluorescence spectroscopy, and 31P NMR are used to identify the formation of the complex, followed by a crystal structure analysis carried out to elucidate the active-site structure of trichosanthin. The determination of germinal vesicle breakdown indicates that the complex does not, at least for abortion-inducing activity, result in competitive inhibition to the protein.
Xiong
J P
JP
Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences.
Xia
Z X
ZX
Wang
Y
Y
D000020:Abortifacient Agents, Nonsteroidal / Q000737:chemistry; D000034:Abortion, Veterinary / Q000139:chemically induced; D000818:Animals; D002023:Bufo bufo; D018360:Crystallography, X-Ray; D004355:Drug Stability; D005260:Female; D009682:Magnetic Resonance Spectroscopy; D009249:NADP / Q000737:chemistry* / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects; D010758:Phosphorus; D011247:Pregnancy; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D015978:Trichosanthin / Q000737:chemistry* / Q000494:pharmacology
D000020:Abortifacient Agents, Nonsteroidal; D010758:Phosphorus; D009249:NADP; D015978:Trichosanthin