Isolation of influenza A and B viruses in HeLa cells.
The HeLa cell line which is one of the most popular cell lines was shown to be suitable for isolation of types A (H3N2) and B influenza viruses from throat washings of patients. Sixty-nine and 67 out of 147 throat washings taken from patients during the period from January to April 1994, were positive for influenza A virus in HeLa cells and MDCK cells respectively. Seven out of 10 throat washings taken between January and March, 1993, were positive for influenza B virus in MDCK. Of these 7, 4 were also positive for HeLa cells.
Yamanaka
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T
Divison of Virology, Public Health Laboratory of Chiba Prefecture, Japan.
Shirasawa
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H
Yoshizawa
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S
D000818:Animals; D002460:Cell Line / Q000821:virology; D003588:Cytopathogenic Effect, Viral; D004285:Dogs; D004848:Epithelium; D005069:Evaluation Studies as Topic; D005289:Ferrets / Q000097:blood / Q000276:immunology; D006367:HeLa Cells / Q000821:virology*; D006801:Humans; D009980:Influenza A virus / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology; D009981:Influenza B virus / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology; D007668:Kidney; D010614:Pharynx / Q000821:virology*; D014776:Virus Cultivation; D014779:Virus Replication