title: Clusters of modular regulatory elements at DNA replication origins.
abstract: To study the specificity of eukaryotic origins of replication (ori), we have isolated a fraction of mouse DNA enriched in replication initiation sequences (RIS), and cloned and characterised some of these RIS. The sequences of three RIS were analysed for the presence of sequence elements common to other known eukaryotic ori. It was found that the three RIS were A+T rich and contained a number of sequence elements that may function in the initiation of DNA replication. The data support the idea that mammalian ori are organised from modular sequence elements.
authors.affiliation: Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
authors.forename: M
authors.initials: M
authors.lastname: Gencheva
authors.forename: B
authors.initials: B
authors.lastname: Anachkova
mesh_terms: D000818:Animals; D001483:Base Sequence; D002286:Carcinoma, Ehrlich Tumor / Q000235:genetics; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012045:Regulatory Sequences, Nucleic Acid; D018741:Replication Origin; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics
chemical_list: D004273:DNA, Neoplasm; D013762:Tetrahydrofolate Dehydrogenase