Indenoindole depresses lipofuscin formation in cultured neonatal rat myocardial cells.
Lipofuscin accumulation in cultured rat myocardial cells is considered an index of intra-lysosomal oxidative reactions and was registered by autofluorescence measurements. Lipofuscinogenesis in secondary lysosomes is thought to be a consequence of Fenton reactions, and is much enhanced by oxidative stress obtained by culturing the cells in an atmosphere containing 40% oxygen. The influence of the synthetic antioxidant indenoindole (DHII), as compared to control cells, was a dose-responsive depression of lipofuscinogenesis to a degree of 19% and 17% with 20 microM DHII and to 25% and 23% with 40 microM DHII after 7 and 14 days in culture, respectively. This demonstrates a significant quenching of oxidative stress and suggests the therapeutic value of DHII and related antioxidants in protecting against oxygen radical-related diseases. It is also suggested that neonatal cardiac myocytes in culture are a suitable model system for the evaluation of oxygen radical-induced myocardial damage.
Marzabadi
M R
MR
Department of Pathology II, Linköping University, Sweden.
Jones
C
C
Rydström
J
J
D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D000975:Antioxidants / Q000494:pharmacology*; D002478:Cells, Cultured; D003864:Depression, Chemical; D005069:Evaluation Studies as Topic; D006321:Heart / Q000187:drug effects*; D007211:Indoles / Q000494:pharmacology*; D008062:Lipofuscin / Q000096:biosynthesis*; D009206:Myocardium / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D007211:Indoles; D008062:Lipofuscin; D014810:Vitamin E; C057286:indenoindole