[Inhibitory effects of calcium channel antagonist on the proliferation of vascular smooth muscle cells induced by adrenoceptor activators].
In cultured vascular smooth muscle cells (VSMC) of SD rat, it was demonstrated that 10(-6) - 10(-5) mol/L NE and 10(-7) - 10(-5) mol/L isoproterenol stimulated proliferation and 3H-TdR incorporation of the cells in a dose dependent manner. These effects might be significantly inhibited by co-incubation with either alpha-receptor blocker phentolamine (10(-6) mol/L) or beta-receptor blocker propranolol (10(-5) mol/L). It was also found that cell counting and 3H-TdR incorporation were markedly decreased after incubation nifedipine (10(-6) mol/L) or verapamil (10(-6) mol/L) with NE (P < 0.01 as compared to that of NE group). It was further observed that nifedipine and verapamil inhibited DNA synthesis and proliferation of VSMC induced by isoproterenol.
Li
Y
Y
Department of Pathophysiology, Fourth Military Medical University, Xi'an.
Wang
R
R
Lin
S X
SX
Jia
B
B
Song
K M
KM
D000322:Adrenergic Agonists / Q000494:pharmacology*; D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects*; D009543:Nifedipine / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014700:Verapamil / Q000494:pharmacology
D000322:Adrenergic Agonists; D002121:Calcium Channel Blockers; D011433:Propranolol; D014700:Verapamil; D009543:Nifedipine; D010646:Phentolamine