title: The gtcRS operon coding for two-component system regulatory proteins is located adjacent to the grs operon of Bacillus brevis.
abstract: We identified, cloned and sequenced an operon comprising of two genes gtcR and gtcS located adjacent to the grs operon of Bacillus brevis, which encodes the multienzymes involved in the biosynthesis of the peptide antibiotic gramicidin S. The transcription initiation site of the gtcRS operon was determined in Bacillus brevis and Bacillus subtilis. The encoded proteins GtcR and GtcS were identified as members of the two-component system family of signal transducing proteins.
authors.affiliation: Philipps Universität Marburg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Turgay
authors.forename: M A
authors.initials: MA
authors.lastname: Marahiel
mesh_terms: D000591:Amino Acid Isomerases / Q000235:genetics; D000595:Amino Acid Sequence; D001407:Bacillus / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004755:Enterobacteriaceae / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D005809:Genes, Regulator; D006096:Gramicidin / Q000096:biosynthesis; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics; D009876:Operon; D010453:Peptide Synthases / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; C096180:GtcR protein, Bacillus brevis; C096181:GtcS protein, Bacillus brevis; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D006096:Gramicidin; D011494:Protein Kinases; D000591:Amino Acid Isomerases; D010453:Peptide Synthases; C020831:gramicidin-S-synthetase 2