Molecular cloning of a widely expressed human homologue for the Drosophila trp gene.
The Drosophila transient receptor potential (trp) gene and its homologue, trpl, have been suggested to mediate calcium entry during the insect's phototransduction process. We isolated a human cDNA, human trp-1 (Htrp-1), encoding a polypeptide of 793 amino acids that is 37% identical (62% similar) to Drosophila trp and trpl. Northern analysis showed that the Htrp-1 transcript is approximately 5.5 kb and expressed in most human tissues, with higher amounts in ovary, testis, heart, and brain. Isolation of Htrp-1 suggests that a trp-type protein is present in mammals and should provide a useful tool in studying calcium-depletion induced calcium influx processes.
Zhu
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X
Department of Anesthesiology and Biological Chemistry, UCLA School of Medicine 90095-1778, USA.
Chu
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PB
Peyton
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Birnbaumer
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L
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000737:chemistry / Q000235:genetics*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D015870:Gene Expression; D017344:Genes, Insect; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D007301:Insect Hormones / Q000737:chemistry / Q000235:genetics*; D019476:Insect Proteins; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D050052:TRPC Cation Channels; D050051:Transient Receptor Potential Channels
D015220:Calcium Channels; D002148:Calmodulin-Binding Proteins; D018076:DNA, Complementary; D029721:Drosophila Proteins; D007301:Insect Hormones; D019476:Insect Proteins; D008565:Membrane Proteins; D050052:TRPC Cation Channels; D050051:Transient Receptor Potential Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; C063169:trp protein, Drosophila; C074192:trpl protein, Drosophila; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium