title: Chemical techniques employed for the primary structural analysis of proteins and peptides.
abstract: This chapter summarises modern microchemical approaches to the purification, identification and primary structure analysis of peptides and proteins. Discussion of high-sensitivity purification methods is restricted to two-dimensional polyacrylamide gel electrophoresis (2-DE) and microbore/capillary column reversed-phase high-performance liquid chromatography (RP-HPLC). Associated techniques are discussed, particularly with respect to analysis of the products with current automated amino acid sequencers and mass spectrometers.
authors.affiliation: Joint Protein Structure Laboratory, Ludwig Institute for Cancer Research (Melbourne Branch) Parkville, Victoria Australia.
authors.forename: A S
authors.initials: AS
authors.lastname: Inglis
authors.forename: G E
authors.initials: GE
authors.lastname: Reid
authors.forename: R J
authors.initials: RJ
authors.lastname: Simpson
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D017421:Sequence Analysis
chemical_list: D000596:Amino Acids; D010455:Peptides; D011506:Proteins