Xanthine analysis in biological fluids by capillary electrophoresis.
Xanthine, a precursor of uric acid, is measured here in serum, urine, and cerebrospinal fluids by capillary electrophoresis (CE) after deproteinization with acetonitrile. The migration time is about 7.5 min with a minimum detection limit of 0.4 mg/l. Different purines and pyrimidines did not interfere with the determination. The method demonstrates the suitability of the CE for determination of small molecules present in a complex matrix at levels of ca. 1mg/l. It also demonstrates that acetonitrile deproteinization is a simple and effective method for preparing samples for CE, allowing a large volume to be introduced into the capillary.
Shihabi
Z K
ZK
Department of Pathology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157, USA.
Hinsdale
M E
ME
Bleyer
A J
AJ
D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D006168:Guinea Pigs; D006801:Humans; D008099:Liver / Q000737:chemistry; D012015:Reference Standards; D015203:Reproducibility of Results; D014970:Xanthines / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000652:urine
D014970:Xanthines