title: Human papillomavirus type 11 transcripts are present at low abundance in latently infected respiratory tissues.
abstract: Respiratory tract tissues containing latent human papillomavirus (HPV) 11 were analyzed by reverse transcription-polymerase chain reaction for the presence of viral-specific RNA from the early region of the genome and compared to a similar analysis of laryngeal papillomas. Latently infected tissue contained low-abundance transcripts that could code for E1 and E2 proteins, but lacked evidence of spliced transcripts for the E6 and E7 proteins. Both latently infected tissue and papilloma tissue contained low-abundance antisense transcripts. Cultured cells infected with HPV 11 virions or transfected with HPV DNA, and cells derived from latently infected tissue, expressed transcripts similar to those seen in papillomas, but at a lower abundance. We postulate that latency is determined by the absence of or limiting levels of critical viral proteins.
authors.affiliation: Department of Otolaryngology and Communication Disorders, Long Island Jewish Medical Center, New Hyde Park, New York 11040, USA.
authors.forename: A
authors.initials: A
authors.lastname: Maran
authors.forename: C A
authors.initials: CA
authors.lastname: Amella
authors.forename: T P
authors.initials: TP
authors.lastname: Di Lorenzo
authors.forename: K J
authors.initials: KJ
authors.lastname: Auborn
authors.forename: L B
authors.initials: LB
authors.lastname: Taichman
authors.forename: B M
authors.initials: BM
authors.lastname: Steinberg
mesh_terms: D002478:Cells, Cultured; D004279:DNA, Viral; D004268:DNA-Binding Proteins / Q000032:analysis; D006801:Humans; D007822:Laryngeal Neoplasms / Q000821:virology; D010212:Papilloma / Q000821:virology; D027383:Papillomaviridae / Q000235:genetics / Q000502:physiology*; D030361:Papillomavirus Infections / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D016372:RNA, Antisense / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase; D012137:Respiratory System / Q000821:virology*; D012680:Sensitivity and Specificity; D014162:Transfection; D014412:Tumor Virus Infections / Q000821:virology; D014764:Viral Proteins / Q000032:analysis; D014771:Virion; D017735:Virus Latency / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; C084304:E1 protein, Human papillomavirus type 11; D016372:RNA, Antisense; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase