Intercellular adhesion molecule-2 (CD102) binds to the leukocyte integrin CD11b/CD18 through the A domain.
The interactions between the leukocyte-specific beta 2-integrins cluster of differentiation (CD) Ag CD11/CD18 and their ligands, the intercellular adhesion molecules (ICAMs), play important roles in many adhesion-dependent leukocyte functions. ICAM-1 is known to be a ligand for both CD11a/CD18 and CD11b/CD18. ICAM-2, whose two extracellular Ig domains show the highest homology to the two NH2-terminal domains of ICAM-1, has been previously shown to be a ligand for CD11a/CD18. We recently found that a 22-amino acid CD11a/CD18-binding peptide, P1, derived from the first domain of ICAM-2, also binds to purified CD11b/CD18. In the present study, we demonstrate that the ICAM-2 protein interacts with CD11b/CD18, and the binding is through the CD11b A domain.
Xie
J
J
Department of Biosciences, University of Helsinki, Finland.
Li
R
R
Kotovuori
P
P
Vermot-Desroches
C
C
Wijdenes
J
J
Arnaout
M A
MA
Nortamo
P
P
Gahmberg
C G
CG
D000818:Animals; D015703:Antigens, CD / Q000737:chemistry / Q000276:immunology*; D001665:Binding Sites; D016466:CHO Cells; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000276:immunology*; D006224:Cricetinae; D006801:Humans; D016177:Macrophage-1 Antigen / Q000737:chemistry / Q000276:immunology*; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; C065289:ICAM2 protein, human; D016177:Macrophage-1 Antigen; D011994:Recombinant Proteins