[Effects of emodin, sennosides and rheum polysaccharides on free calcium in isolated rat liver cells].
The effects of emodin (EMD), sennosides (SEN) and Rheum polysaccharides (RPP) on the free calcium level in the isolated rat liver cells were studied with Ca2+ level 131.60 +/- 37.79 nmol/L in the liver cells. After adding CaCl2 (2 mmol/L) and KCl (120 mmol/L) to liver cell suspension sequentially, the free Ca2+ levels were significantly elevated compared with that of the resting status (P < 0.01). When the liver cells were pretreated with EMD (0.037 mmol/L) for 10 min, in the resting status or using the above doses of CaCl2 and KCl, the free Ca2+ levels were significantly increased compared with that of the control groups (P < 0.01). On the contrary, after administration of SEN (0.046-0.092 mmol/L) and RPP (0.1-0.2 mg/ml), the free Ca2+ levels were obviously decreased compared with that of the control groups (P < 0.01). Furthermore, the inhibitory effect was dose dependent. The opposite effects of the different active ingredients of rhubarb (EMD,SEN,RPP) on the free Ca2+ levels suggested that rhubarb has many kinds of regulatory function in the liver cells.
Lin
X Z
XZ
Dept. of Pharmacology, Tianjin University of Medical Sciences.
Ma
D L
DL
Cui
R F
RF
D000818:Animals; D000880:Anthraquinones / Q000494:pharmacology*; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D002478:Cells, Cultured; D004642:Emodin / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D010946:Plants, Medicinal; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D012250:Rheum / Q000737:chemistry; D012676:Senna Extract; D000081226:Sennosides
D000880:Anthraquinones; D011134:Polysaccharides; D000081226:Sennosides; D012676:Senna Extract; D004642:Emodin; D002118:Calcium