title: Tumor necrosis factors alpha and beta protect neurons against amyloid beta-peptide toxicity: evidence for involvement of a kappa B-binding factor and attenuation of peroxide and Ca2+ accumulation.
abstract: In Alzheimer disease (AD) the amyloid beta-peptide (A beta) accumulates in plaques in the brain. A beta can be neurotoxic by a mechanism involving induction of reactive oxygen species (ROS) and elevation of intracellular free calcium levels ([Ca2+]i). In light of evidence for an inflammatory response in the brain in AD and reports of increased levels of tumor necrosis factor (TNF) in AD brain we tested the hypothesis that TNFs affect neuronal vulnerability to A beta. A beta-(25-35) and A beta-(1-40) induced neuronal degeneration in a concentration- and time-dependent manner. Pretreatment of cultures for 24 hr with TNF-beta or TNF-alpha resulted in significant attenuation of A beta-induced neuronal degeneration. Accumulation of peroxides induced in neurons by A beta was significantly attenuated in TNF-pretreated cultures, and TNFs protected neurons against iron toxicity, suggesting that TNFs induce antioxidant pathways. The [Ca2+]i response to glutamate (quantified by fura-2 imaging) was markedly potentiated in neurons exposed to A beta, and this action of A beta was suppressed in cultures pretreated with TNFs. Electrophoretic mobility-shift assays demonstrated an induction of a kappa beta-binding activity in hippocampal cells exposed to TNFs. Exposure of cultures to I kappa B (MAD3) antisense oligonucleotides, a manipulation designed to induce NF-kappa B, mimicked the protection by TNFs. These data suggest that TNFs protect hippocampal neurons against A beta toxicity by suppressing accumulation of ROS and Ca2+ and that kappa B-dependent transcription is sufficient to mediate these effects. A modulatory role for TNF in the neurodegenerative process in AD is proposed.
authors.affiliation: Sanders-Brown Research Center on Aging, University of Kentucky, Lexington 40536-0230, USA.
authors.forename: S W
authors.initials: SW
authors.lastname: Barger
authors.forename: D
authors.initials: D
authors.lastname: Hörster
authors.forename: K
authors.initials: K
authors.lastname: Furukawa
authors.forename: Y
authors.initials: Y
authors.lastname: Goodman
authors.forename: J
authors.initials: J
authors.lastname: Krieglstein
authors.forename: M P
authors.initials: MP
authors.lastname: Mattson
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D018698:Glutamic Acid / Q000633:toxicity; D006801:Humans; D007700:Kinetics; D008233:Lymphotoxin-alpha / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D009498:Neurotoxins / Q000633:toxicity*; D009838:Oligodeoxyribonucleotides; D010446:Peptide Fragments / Q000633:toxicity; D010545:Peroxides / Q000378:metabolism*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
chemical_list: D016229:Amyloid beta-Peptides; D008233:Lymphotoxin-alpha; D016328:NF-kappa B; D009498:Neurotoxins; D009838:Oligodeoxyribonucleotides; D010446:Peptide Fragments; D010545:Peroxides; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D018698:Glutamic Acid; D002118:Calcium