title: Proteolytic activity of proteasome on myofibrillar structures.
abstract: The physiologic function of proteasome remains unclear. Evidence suggests a role in degradation of ubiquitin-protein conjugates, MHC antigen presentation, and some specificity of substrate within certain cell types. To explore further the properties of proteasome we have examined its effect on a well defined structure, the myofibril. We find that despite its large size (20S) proteasome is able to degrade myofibrils and intact, permeabilized muscle fibrils. The proteins degraded showed some specificity because actin, myosin and desmin were degraded faster than alpha-actinin, troponin T and tropomyosin. Changes in ultrastructure were slow and included a general loss of structure with Z and I bands effected before the M band and costameres.
authors.affiliation: INRA, Station de Recherche sur la Viande, St Genes Champanelle, France.
authors.forename: R G
authors.initials: RG
authors.lastname: Taylor
authors.forename: C
authors.initials: C
authors.lastname: Tassy
authors.forename: M
authors.initials: M
authors.lastname: Briand
authors.forename: N
authors.initials: N
authors.lastname: Robert
authors.forename: Y
authors.initials: Y
authors.lastname: Briand
authors.forename: A
authors.initials: A
authors.lastname: Ouali
mesh_terms: D000818:Animals; D002417:Cattle; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism*; D018485:Muscle Fibers, Skeletal / Q000378:metabolism / Q000648:ultrastructure; D009124:Muscle Proteins / Q000378:metabolism; D009210:Myofibrils / Q000378:metabolism* / Q000648:ultrastructure; D046988:Proteasome Endopeptidase Complex
chemical_list: D009097:Multienzyme Complexes; D009124:Muscle Proteins; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex