[T cell receptor V beta repertoire of locoregional lymphocytes in malignant effusions].
We analyzed the T cell receptor (TCR) V beta repertoire of lymphocytes obtained from patients with malignant effusion treated by locoregional immunotherapy. Polymerase chain reaction using a panel of V beta subfamily specific oligonucleotide primers(V beta 1-20) was employed after reverse transcription of mRNA isolated from the locoregional cells. No major oligoclonality of TCR repertoire was observed in effusion lymphocytes before the treatment. The expression level of V beta 13.1 repertoire was significantly higher in effusion lymphocytes than in peripheral blood lymphocytes before treatment. V beta 20 gene expression increased significantly after locoregional administration of OK-432. It is suggested that TCR V beta 13.1 may be responsible for effusion lymphocytes and TCR V beta 20 for OK-432-related antigenic stimulus.
Miyahara
E
E
Department of Surgical Oncology, Hiroshima University.
Yamaguchi
Y
Y
Hihara
J
J
Noma
K
K
Funakoshi
M
M
Takashima
I
I
Sawamura
A
A
Toge
T
T
D000328:Adult; D000368:Aged; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D007167:Immunotherapy; D008297:Male; D008875:Middle Aged; D010844:Picibanil / Q000627:therapeutic use*; D016066:Pleural Effusion, Malignant / Q000276:immunology* / Q000628:therapy; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology*
D000970:Antineoplastic Agents; D016693:Receptors, Antigen, T-Cell, alpha-beta; D010844:Picibanil