The platelet endothelial cell adhesion molecule-1 (PECAM1) contributes to endothelial barrier function.
In this study we have analyzed the role of the platelet-endothelial cell adhesion molecule-1 (PECAM1) in vascular barrier function. PECAM1 is an immunoglobulin gene superfamily member expressed by endothelial cells at the cell boundaries. Macromolecule permeability assays performed on cell monolayers that express native or transfected PECAM1, indicated that the molecule participates in the establishment and maintenance of vascular barrier function in vitro. This hypothesis was confirmed by the finding that in vivo injection of the specific monoclonal antibody directed against the murine vascular PECAM1 led to a detectable leakage of hepatic and renal blood vessels.
Ferrero
E
E
Laboratory of Adoptive Immunotherapy, Scientific Institute San Raffaele, Milan, Italy.
Ferrero
M E
ME
Pardi
R
R
Zocchi
M R
MR
D000818:Animals; D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics / Q000502:physiology*; D002199:Capillary Permeability; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D004730:Endothelium, Vascular / Q000502:physiology*; D006801:Humans; D051379:Mice; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011994:Recombinant Proteins; D014162:Transfection; D014471:Umbilical Veins
D000911:Antibodies, Monoclonal; D015214:Antigens, Differentiation, Myelomonocytic; D015815:Cell Adhesion Molecules; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D011994:Recombinant Proteins