Interleukin-1 and oncostatin M in combination promote the release of collagen fragments from bovine nasal cartilage in culture.
Interleukin-1 (IL-1) and Oncostatin M (OM) induce a rapid and reproducible release of proteoglycan and collagen fragments from bovine nasal cartilage in culture. Over 90% of the total collagen was released by day 14 compared to a variable release with IL-1 alone. This release was accompanied by the appearance of collagenolytic activity in the medium that cleaved collagen specifically at the one quarter/three quarter position. Tissue inhibitor of metalloproteinases (TIMP-1) activity was low or absent in media from resorbing tissue. The breakdown of cartilage collagen could be prevented by the addition of BB94, a specific matrix metalloproteinase (MMP) inhibitor. These results suggest that T-cell/macrophage products within inflammed joints can interact with pro-inflammatory cytokines and lead to the rapid destruction of connective tissue collagen.
Cawston
T E
TE
Rheumatology Research Unit, Addenbrooke's Hospital, Cambridge, UK.
Ellis
A J
AJ
Humm
G
G
Lean
E
E
Ward
D
D
Curry
V
V
D000818:Animals; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D007375:Interleukin-1 / Q000008:administration & dosage / Q000494:pharmacology*; D007700:Kinetics; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors; D009666:Nose; D053683:Oncostatin M; D010455:Peptides / Q000008:administration & dosage / Q000494:pharmacology*; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology; D011509:Proteoglycans / Q000378:metabolism; D013876:Thiophenes / Q000494:pharmacology; D019714:Tissue Inhibitor of Metalloproteinases
D006023:Glycoproteins; D006025:Glycosaminoglycans; D007375:Interleukin-1; D010455:Peptides; D011480:Protease Inhibitors; D011509:Proteoglycans; D013876:Thiophenes; D019714:Tissue Inhibitor of Metalloproteinases; D053683:Oncostatin M; D010649:Phenylalanine; D003094:Collagen; C080985:batimastat; D008666:Metalloendopeptidases