Rat mitochondrial mtDNA-binding proteins to inter-specifically conserved sequences in the displacement loop region of vertebrate mtDNAs.
We describe two new inter-specifically conserved sequences named Mt5 and Mt6 elements which are around the termination associated sequence (TAS) at the 3' end of the displacement loop (D-loop) region of vertebrate mtDNAs. We employed gel mobility shift assay to search for D-loop-binding proteins using partially purified rat mitochondrial mtDNA-binding proteins and 10 human DNA fragments covering the entire D-loop region as probe DNAs. Competition binding assays indicated the presence of four different rat mitochondrial D-loop-binding proteins. Each of the rat proteins bound, in a sequence-specific manner, to a probe DNA containing an inter-specifically conserved sequence; TAS, Mt5, B block, and a A+T-rich sequence overlapping the conserved sequence block I.
Kumar
S
S
Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya, Japan.
Suzuki
H
H
Onoue
S
S
Suzuki
S
S
Hattori
N
N
Ozawa
T
T
D000818:Animals; D001483:Base Sequence; D001667:Binding, Competitive; D017124:Conserved Sequence / Q000235:genetics*; D015342:DNA Probes; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D006801:Humans; D008928:Mitochondria / Q000378:metabolism; D008969:Molecular Sequence Data; D051381:Rats; D013045:Species Specificity
D015342:DNA Probes; D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins