Liquid chromatographic determination of furazolidone in swine serum and avian egg.
A rapid, simple, and accurate method for determination of furazolidone (FZ) in swine serum and avian egg using liquid chromatography (LC) with a 358 nm ultraviolet-visible spectrophotometric detector is described. After liquid-liquid extraction of sample with ethyl acetate using Extrelut-3, the extract is evaporated, redissolved in 40% acetonitrile, and injected directly into the chromatograph. The antibiotic can be analyzed within 30 min. Within-day recoveries for swine serum and avian egg spiked with FZ at 1 ppm were 90.0 and 88.1%, respectively, with coefficients of variation of 3.52 and 3.88%, respectively. Between days recoveries for the 1 ppm samples were 87.2 and 87.0%, with coefficients of variation of 3.10 and 4.29%, respectively. Determination of FZ also was performed by LC/mass spectrometry (MS) with an atmospheric-pressure chemical-ionization interface (APCI) system. The LC/MS-APCI system is more applicable for qualitative analysis than quantitative analysis because the drug detection limit (about 0.1 microgram/L) is almost the same as that of the LC-UV detector.
Yoshida
K
K
Miyazaki University, Faculty of Agriculture, Department of Veterinary Public Health, Japan.
Kondo
F
F
D000818:Animals; D002138:Calibration; D002853:Chromatography, Liquid; D004531:Eggs / Q000032:analysis*; D005664:Furazolidone / Q000032:analysis* / Q000097:blood*; D011200:Poultry / Q000033:anatomy & histology*; D013056:Spectrophotometry, Ultraviolet; D013552:Swine / Q000097:blood*
D005664:Furazolidone