Analysis of underivatized amino acids by capillary electrophoresis using constant potential amperometric detection.
A mixture of native (underivatized) amino acids is separated by capillary electrophoresis under alkaline conditions (pH approximately 12) and amperometrically detected with a copper-disk microelectrode. A simple design facilitates capillary-electrode alignment without the need for micropositioning equipment. The limits of detection for the amino acids are in the low microM concentration range, and the signal response is linear over 2-3 orders of magnitude. This procedure is applied to analyze the amino acid hydrolysis products from cytochrome c.
Guo
Y
Y
Department of Chemistry, State University of New York at Buffalo 14260-3000, USA.
Colón
L A
LA
Dadoo
R
R
Zare
R N
RN
D000596:Amino Acids / Q000032:analysis*; D003300:Copper; D003574:Cytochrome c Group / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods*; D006868:Hydrolysis; D008839:Microelectrodes; D010710:Phosphates / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry
D000596:Amino Acids; D003574:Cytochrome c Group; D010710:Phosphates; D012972:Sodium Hydroxide; D003300:Copper; C018279:sodium phosphate