title: Localisation of metallothionein isoform mRNAs in rat hepatoma (H4) cells.
abstract: The localisation of metallothionein isoform mRNAs in rat hepatoma (H4) cells was investigated using two approaches, namely Northern hybridisation of total RNA extracted from free, cytoskeletal-bound and membrane-bound polysomes isolated by a sequential detergent/salt extraction procedure and in situ hybridisation. The cytoskeletal-bound polysomes were enriched in metallothionein-I (MT-I) and c-myc mRNAs but showed a significantly lower enrichment in MT-II mRNA. These findings indicate that the MT-I mRNA is localised to the cytoskeleton during translation. In situ hybridisation using a biotin-labelled oligonucleotide probe revealed a predominantly perinuclear localisation for the MT-I mRNA.
authors.affiliation: Division of Biochemical Sciences, Rowett Research Institute, Aberdeen, Scotland, UK.
authors.forename: P
authors.initials: P
authors.lastname: Mahon
authors.forename: J
authors.initials: J
authors.lastname: Beattie
authors.forename: L A
authors.initials: LA
authors.lastname: Glover
authors.forename: J
authors.initials: J
authors.lastname: Hesketh
mesh_terms: D000199:Actins / Q000032:analysis; D000818:Animals; D015152:Blotting, Northern; D002458:Cell Fractionation; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure; D003600:Cytosol / Q000378:metabolism / Q000648:ultrastructure; D017403:In Situ Hybridization; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008668:Metallothionein / Q000032:analysis / Q000096:biosynthesis*; D011132:Polyribosomes / Q000378:metabolism / Q000648:ultrastructure; D012333:RNA, Messenger / Q000032:analysis* / Q000378:metabolism; D012334:RNA, Neoplasm / Q000032:analysis / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis
chemical_list: D000199:Actins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014746:Vimentin; D008668:Metallothionein