title: Evidence for elongation of the helical pitch of the RecA filament upon ATP and ADP binding using small-angle neutron scattering.
abstract: Structural changes of the RecA filament upon binding of cofactors have been investigated by small-angle neutron scattering. Both ATP and ADP increased the helical pitch of the RecA homopolymer, which is observed to be 7 nm in the absence of any cofactor. The binding of ATP altered the pitch to 9 nm, whereas the binding of ADP only produced a pitch of 8.2 nm. The pitch determined for the RecA complex with the ATP analog adenosine 5'-[gamma-thio]triphosphate was similar to that found with ATP. Thus, at least three, somewhat different. RecA helical filamentous structures may form in solution. The binding of DNA to RecA did not alter the pitch significantly, indicating that the cofactor binding is the determining factor for the size of the helical pitch of the RecA filament. We also found that elongation of the helical pitch is a necessary, but not a sufficient condition, for the coprotease activity of RecA. The presence of acetate or glutamate ions is also required. The pitch of the ADP.RecA filament is in agreement with that found in the crystal structure. This correlation indicates that this structure corresponds to that of the ADP.RecA filament in solution, although this is not the species active in recombination.
authors.affiliation: Groupe d'Etude Mutagénese et Cancérogénèse, Centre National de la Recherche Scientifique Unité 1342, Orsay, France.
authors.forename: C
authors.initials: C
authors.lastname: Ellouze
authors.forename: M
authors.initials: M
authors.lastname: Takahashi
authors.forename: P
authors.initials: P
authors.lastname: Wittung
authors.forename: K
authors.initials: K
authors.lastname: Mortensen
authors.forename: M
authors.initials: M
authors.lastname: Schnarr
authors.forename: B
authors.initials: B
authors.lastname: Nordén
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D009502:Neutrons; D011487:Protein Conformation; D011938:Rec A Recombinases / Q000737:chemistry* / Q000378:metabolism; D012542:Scattering, Radiation
chemical_list: D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D011938:Rec A Recombinases