title: [Identification of spermatozoa L-selectin and two potential pellucida ligands].
abstract: At the molecular level, gamete interactions partly depend on zona pellucida-glycoproteins fucosylation. We show, by immunocytochemistry, the sialyl-lewisx and sialyl-lewisa oligosaccharides on human zonae pellucidae. These epitopes are potential ligands of cell adhesion molecules named selectins which are known to play a role in endothelium-leukocyte interactions. By immunofluorescence, we find the leukocyte selectin (L-selectin) on the spermatozoa head. Preincubation of spermatozoa with an anti-L-selectin monoclonal antibody produces a significant inhibition of zona pellucida tight binding, under hemizona assay conditions. In contrast, preincubation of the zonae pellucidae with anti-sialyl-lewisx or anti-sialyl-lewisa antibodies does not produce a significant inhibition of spermatozoa binding. Western blot analysis of spermatozoa-detergent extracts revealed a band at approximatively 90 kDa with the anti-L-selectin monoclonal antibody. This spermatozoa selectin could play a role in human spermatozoa-zona pellucida binding. Zona-ligands have yet to be precisely defined.
authors.affiliation: INSERM U.419, Institut de biologie, Nantes, France.
authors.forename: H
authors.initials: H
authors.lastname: Lucas
authors.forename: J
authors.initials: J
authors.lastname: Le Pendu
authors.forename: J
authors.initials: J
authors.lastname: Harb
authors.forename: A
authors.initials: A
authors.lastname: Moreau
authors.forename: S
authors.initials: S
authors.lastname: Bercegeay
authors.forename: P
authors.initials: P
authors.lastname: Barrière
mesh_terms: D015153:Blotting, Western; D005455:Fluorescent Antibody Technique; D006801:Humans; D019041:L-Selectin / Q000302:isolation & purification*; D008024:Ligands; D008297:Male; D013077:Sperm Head / Q000737:chemistry*; D015044:Zona Pellucida / Q000737:chemistry*
chemical_list: D008024:Ligands; D019041:L-Selectin
vernacular_title: Identification d'une L-sélectine spermatique et de 2 ligands pellucidaires potentiels.