Negative and positive elements in the promoter region of the human apoferritin L gene.
We have characterized the promoter of the human gene coding for the apoferritin L subunit. Transient transfections of 5' and 3' deletion mutants indicate that the efficiency of the L promoter depends on both negative and positive cis-elements, located upstream and downstream of the transcription start point. DNaseI footprinting analysis of this DNA region revealed the presence of five protected segments. The most upstream one (element 1) corresponds to the negative cis-element and is recognized by factor(s) sharing a GC-sequence specificity. Three positive elements are in the region upstream of the start of transcription; a fifth positive cis-element (element 5) is localized in the first exon of the L gene.
D'Agostino
P
P
Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli 'Federico II', Italy.
Faniello
M C
MC
Quaresima
B
B
Bevilacqua
M A
MA
Tiano
M T
MT
Ammendola
R
R
Cimino
F
F
Costanzo
F
F
D001052:Apoferritins / Q000235:genetics*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D018983:DNA Footprinting; D003850:Deoxyribonuclease I; D005091:Exons; D017353:Gene Deletion; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D014162:Transfection
D009687:Nuclear Proteins; D004247:DNA; D001052:Apoferritins; D003850:Deoxyribonuclease I