Corticotropin expression by human melanocytes in the skin.
Highly dendritic melanocytes have been observed in rapidly proliferating seborrheic keratosis, epidermis overlying melanomas, and in melanomas. On staining for the presence of POMC with monoclonal antibody against human ACTH, the melanocytes show cytoplasmic positivity. Short term organ cultures of whole skin from the marginal zone of vitiligo patients show that 22.7% of controls and 45.5% on dark incubation in adriamycin and 87.5% exposed to a pulse of UV on adriamycin treatment show melanocytes positive for ACTH. The surrounding keratinocytes in the epidermis and in the seborrheic keratosis are negative, whereas in melanomas, isolated groups of melanocytes are positive for ACTH. These findings indicate that ACTH is expressed by the melanocytes in the G2-phase, the activity being enhanced on UV exposure. Thus UV dependent pigmentation is associated with POMC production in human skin. From this work it is evident that the melanocyte network varies the MSH/ACTH levels in correlation with repigmentation and depigmentation in the marginal zone in vitiligo by expressing POMC locally and is related to the UV-sensitivity of the melanocytes.
Iyengar
B
B
Institute of Pathology (ICMR), New Delhi, India.
D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D000911:Antibodies, Monoclonal; D003593:Cytoplasm / Q000737:chemistry; D004317:Doxorubicin / Q000494:pharmacology; D004817:Epidermis / Q000737:chemistry; D016195:G2 Phase; D006801:Humans; D007150:Immunohistochemistry; D017492:Keratosis, Seborrheic / Q000378:metabolism; D008544:Melanocytes / Q000737:chemistry*; D008545:Melanoma / Q000737:chemistry; D009924:Organ Culture Techniques; D011333:Pro-Opiomelanocortin / Q000032:analysis; D012867:Skin / Q000737:chemistry*; D014466:Ultraviolet Rays; D014820:Vitiligo / Q000378:metabolism
D000911:Antibodies, Monoclonal; D011333:Pro-Opiomelanocortin; D004317:Doxorubicin; D000324:Adrenocorticotropic Hormone