CD4 down-modulation by ganglioside and phorbol ester inhibits human herpesvirus 7 infection.
Recently, data demonstrating that CD4 is an essential component of the receptor for human herpesvirus 7 (HHV-7) as well as for human immunodeficiency virus have been accumulating. Since gangliosides and phorbol esters are known to induce selective down-modulation of cell surface CD4 expression, it might be expected that treatment with these agents would interfere with HHV-7 infection of CD4+ T cells. The present study, undertaken to verify this possibility, demonstrated that addition of monosialoganglioside-GM1 or 12-O-tetradecanoylphorbol 13-acetate effectively induced disappearance of CD4 from the cell surface and also reduced HHV-7 infectivity, as judged by the CPE on virus-infected cells and studies of indirect immunofluorescence, TCID50 and semi-quantitative PCR of the HHV-7 genome. Taken together with previous studies, the present data strongly suggest that the CD4 molecule is a critical component of the receptor for HHV-7.
Yasukawa
M
M
First Department of Internal Medicine, Ehime University School of Medicine, Japan.
Inoue
Y
Y
Sada
E
E
Yakushijin
Y
Y
Furukawa
M
M
Fujita
S
S
D000956:Antigens, Viral / Q000032:analysis; D000998:Antiviral Agents / Q000494:pharmacology*; D001483:Base Sequence; D015704:CD4 Antigens / Q000187:drug effects*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D017931:DNA Primers; D015536:Down-Regulation; D005677:G(M1) Ganglioside / Q000494:pharmacology*; D006566:Herpesviridae Infections / Q000821:virology; D016199:Herpesvirus 7, Human / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008969:Molecular Sequence Data; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D000956:Antigens, Viral; D000998:Antiviral Agents; D015704:CD4 Antigens; D017931:DNA Primers; D007166:Immunosuppressive Agents; D005677:G(M1) Ganglioside; D013755:Tetradecanoylphorbol Acetate