Activation of dual T cell signaling pathways by the chemokine RANTES.
The chemokine RANTES induced biphasic mobilization of Ca2+ in T cells. The initial peak, a transient increase in cytosolic Ca2+ mediated by a heterotrimeric guanine nucleotide-binding protein (G protein)--coupled pathway, was associated predominantly with chemotaxis. The second peak, Ca2+ release and sustained influx dependent on protein tyrosine kinases, was associated with a spectrum of cellular responses--Ca2+ channel opening, interleukin-2 receptor expression, cytokine release, and T cell proliferation--characteristic of T cell receptor activation. Other chemokines did not produce these responses. Thus, in addition to inducing chemotaxis, RANTES can act as an antigen-independent activator of T cells in vitro.
Bacon
K B
KB
Department of Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA.
Premack
B A
BA
Gardner
P
P
Schall
T J
TJ
D016227:Benzoquinones; D017252:CD3 Complex / Q000276:immunology; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000378:metabolism; D018946:Chemokine CCL5 / Q000494:pharmacology*; D002634:Chemotaxis, Leukocyte; D002999:Clone Cells; D016207:Cytokines / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D006801:Humans; D047029:Lactams, Macrocyclic; D008213:Lymphocyte Activation; D018408:Patch-Clamp Techniques; D010835:Phytohemagglutinins / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011809:Quinones / Q000494:pharmacology; D018121:Receptors, Cytokine / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000378:metabolism; D017828:Rifabutin / Q000031:analogs & derivatives; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D010566:Virulence Factors, Bordetella / Q000494:pharmacology
D016227:Benzoquinones; D017252:CD3 Complex; D015220:Calcium Channels; D018946:Chemokine CCL5; D016207:Cytokines; D047029:Lactams, Macrocyclic; D010835:Phytohemagglutinins; D011809:Quinones; D018121:Receptors, Cytokine; D015375:Receptors, Interleukin-2; D010566:Virulence Factors, Bordetella; D017828:Rifabutin; C020754:herbimycin; D011505:Protein-Tyrosine Kinases; D019204:GTP-Binding Proteins; D002118:Calcium