Does the sulfhydryl or the adenine moiety of CoA enhance firefly luciferase activity?
Light production by firefly luciferase is limited by product release resulting in flash kinetics. Several compounds (CoA, PPi, and nucleotides) transform the flash-form of light production into continuous light production. The sulfhydryl group of CoA is required; however, since nucleotides are also active, at least two mechanisms (sites) must exist.
Ford
S R
SR
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078-0454, USA.
Buck
L M
LM
Leach
F R
FR
D000227:Adenine Nucleotides / Q000737:chemistry*; D000818:Animals; D003065:Coenzyme A / Q000737:chemistry*; D001517:Coleoptera / Q000201:enzymology; D004229:Dithiothreitol; D004789:Enzyme Activation; D008156:Luciferases / Q000737:chemistry / Q000378:metabolism*; D008163:Luminescent Measurements; D013438:Sulfhydryl Compounds / Q000737:chemistry*
D000227:Adenine Nucleotides; D013438:Sulfhydryl Compounds; D008156:Luciferases; D003065:Coenzyme A; D004229:Dithiothreitol