A hydroxymethylglutaryl-CoA reductase inhibitor synthesized by yeasts.
Screening of different yeast species showed that they are able to synthesize hydroxymethylglutaryl-CoA (HMGCoA) reductase inhibitors. Crude methanol extracts and the purified inhibitors from Pichia labacensis and Candida cariosilignicola were tested for their biological activity on the solubilized microsomal HMGCoA reductase from Chinese hamster ovary cells. Identification of the inhibitors was studied by thin layer chromatography, high pressure liquid chromatography and mass spectroscopy.
Gunde-Cimerman
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National Institute of Chemistry, Ljubljana, Slovenia.
Plemenitas
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Cimerman
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D000818:Animals; D016466:CHO Cells; D002175:Candida / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006224:Cricetinae; D004791:Enzyme Inhibitors / Q000378:metabolism*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000096:biosynthesis*; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent; D013058:Mass Spectrometry; D008861:Microsomes / Q000201:enzymology; D010843:Pichia / Q000378:metabolism; D015003:Yeasts / Q000201:enzymology*
D004791:Enzyme Inhibitors; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D006903:Hydroxymethylglutaryl CoA Reductases; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent