title: Limited TCR repertoire of infiltrating T cells in the kidneys of Sjögren's syndrome patients with interstitial nephritis.
abstract: To analyze the mechanism of interstitial nephritis in patients with Sjörgren's syndrome (SS), we examined the TCR repertoire of infiltration T cells in kidney, labial salivary glands, and PBLs using a PCR. The repertoire of the TCR V beta gene on infiltrating T cells from the kidneys of SS patients was more restricted than those on infiltrating T cells in labial salivary glands and PBL. The TCR V beta 2 gene was expressed predominantly in six of seven (86%) SS patients. Junctional sequences of cDNAs encoding the V beta 2 gene on infiltrating T cells in the kidneys of five SS patients showed that some of the cells expanded clonally, indicating Ag-driven stimulation rather than superantigen-induced proliferation. The same V beta 2 clones in the kidney were not detected in labial salivary glands of the same SS patients; the conserved amino acid (arginine at position 96) in the CDR3 region of the V beta 2 gene was found at a frequency of 48.0% in the kidney, whereas it was detected in only 15.4% of the clones in lips. In conclusion, these findings suggest the possibility that T cells that infiltrate the kidneys of SS patients with interstitial nephritis might recognize different autoantigens than those that infiltrate labial salivary glands.
authors.affiliation: Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Murata
authors.forename: Y
authors.initials: Y
authors.lastname: Kita
authors.forename: A
authors.initials: A
authors.lastname: Sakamoto
authors.forename: I
authors.initials: I
authors.lastname: Matsumoto
authors.forename: R
authors.initials: R
authors.lastname: Matsumura
authors.forename: T
authors.initials: T
authors.lastname: Sugiyama
authors.forename: M
authors.initials: M
authors.lastname: Sueishi
authors.forename: K
authors.initials: K
authors.lastname: Takabayashi
authors.forename: I
authors.initials: I
authors.lastname: Iwamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Saitoh
authors.forename: K
authors.initials: K
authors.lastname: Nishioka
authors.forename: T
authors.initials: T
authors.lastname: Sumida
mesh_terms: D000595:Amino Acid Sequence; D015496:CD4-Positive T-Lymphocytes / Q000473:pathology; D002465:Cell Movement; D006801:Humans; D007668:Kidney / Q000276:immunology* / Q000473:pathology; D008969:Molecular Sequence Data; D009395:Nephritis, Interstitial / Q000097:blood / Q000276:immunology* / Q000473:pathology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D012470:Salivary Glands, Minor / Q000276:immunology / Q000473:pathology; D012859:Sjogren's Syndrome / Q000097:blood / Q000276:immunology* / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000473:pathology*
chemical_list: D016693:Receptors, Antigen, T-Cell, alpha-beta