title: Expression of the c-fos transcription factor in the rat auditory pathway following postnatal auditory deprivation.
abstract: As an animal model for inborn hearing loss rat pups were reared in a sound-proof chamber from birth until age 21 days. In addition, pinnae were bilaterally sutured closed to reduce any influence of ambient sound. At the end of the sound deprivation, outer ear channels were reopened. Since previous studies failed to show any difference in the number or morphology of neurons in the auditory pathway in bilaterally sound-deprived animals, expression of c-fos protein was used as a functional marker to map trans-synaptic information transfer in the auditory pathway with cellular resolution. At day 21 sound-deprived rats and untreated controls were stimulated with pure tones of 8kHz for 5min at different sound pressure levels. Acoustic stimulation induced c-fos in both parts of the cochlear nucleus, superior olivary complex and inferior colliculus. Compared to untreated rats, deprivation reduced the number of c-fos labeled neurons in the dorsal and ventral part of the cochlear nucleus and inferior colliculus by 58% and 30%, respectively, following low sound pressure levels (90dB). In contrast, high sound pressure levels (120dB) increased the number of c-fos labeled neurons in these areas and evoked only minor differences in the number of labeled neurons in both untreated and sound deprived rats.
authors.affiliation: Universitäts-HNO-Klinik, Theodor-Kutzer-Ufer, Mannheim, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Keilmann
authors.forename: T
authors.initials: T
authors.lastname: Herdegen
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D000831:Animals, Newborn; D001309:Auditory Threshold; D017626:Cochlear Nucleus / Q000166:cytology / Q000502:physiology* / Q000503:physiopathology*; D016762:Genes, fos / Q000235:genetics*; D006311:Hearing Disorders / Q000175:diagnosis* / Q000503:physiopathology*; D007245:Inferior Colliculi / Q000166:cytology / Q000502:physiology* / Q000503:physiopathology*; D008297:Male; D051381:Rats; D014158:Transcription, Genetic