title: Comparison of two selective media and a membrane filter technique for isolation of Campylobacter species from diarrhoeal stools.
abstract: Diarrhoeal stool specimens from 415 patients were examined for Campylobacter spp. by culture on charcoal cefoperazone deoxycholate agar (CCDA), Skirrow medium and Columbia blood agar overlaid with a 0.65 micron pore size membrane filter. Forty-eight Campylobacter strains were isolated from 45 (10.8%) specimens by all media; 44 were Campylobacter jejuni (91.7%), three were Campylobacter coli (6.3%) and one was Campylobacter hyointestinalis (2.0%). The percentages of Campylobacter-positive specimens isolated on Skirrow medium, CCDA and the membrane filter were 62, 82 and 95%, respectively. The recovery of more Campylobacter spp. from the same stool sample was achieved by the membrane filter method only. The highest isolation rate (100%) was observed when culture on CCDA and the membrane filter method were combined.
authors.affiliation: Department of Clinical Microbiology, General Hospital Hospital Umberto 1st-Torrette, Ancona, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Piersimoni
authors.forename: S
authors.initials: S
authors.lastname: Bornigia
authors.forename: L
authors.initials: L
authors.lastname: Curzi
authors.forename: G
authors.initials: G
authors.lastname: De Sio
mesh_terms: D001431:Bacteriological Techniques; D002167:Campylobacter / Q000254:growth & development / Q000302:isolation & purification*; D003470:Culture Media; D003967:Diarrhea / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D012680:Sensitivity and Specificity
chemical_list: D003470:Culture Media