title: Isolation, characterization and chromosomal localization of a human pseudogene for hexokinase II.
abstract: A processed pseudogene for hexokinase II (HKII), the first such reported for a member of the hexokinase gene family, was isolated from a human genomic library by using a rat HKII cDNA as a probe. The pseudogene contains a region that is identical to the open reading frame of the human HKII cDNA at 97% of the nucleotide positions, but it contains several frameshift mutations, small deletions and insertions, and several stop codons. The human HKII pseudogene is located on the X chromosome and is integrated into a long interspersed nuclear repetitive DNA element (LINE). We estimate that this integration event occurred approximately 14-16 Myr (million years) ago.
authors.affiliation: Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
authors.forename: H
authors.initials: H
authors.lastname: Ardehali
authors.forename: R L
authors.initials: RL
authors.lastname: Printz
authors.forename: S
authors.initials: S
authors.lastname: Koch
authors.forename: J A
authors.initials: JA
authors.lastname: Phillips
authors.forename: D K
authors.initials: DK
authors.lastname: Granner
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D005260:Female; D016368:Frameshift Mutation; D015698:Genomic Library; D006593:Hexokinase / Q000235:genetics*; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016366:Open Reading Frames; D017354:Point Mutation; D011544:Pseudogenes; D051381:Rats; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D014960:X Chromosome
chemical_list: D004251:DNA Transposable Elements; D018076:DNA, Complementary; D006593:Hexokinase