[Genetic study of plasmid pVM82 which contributes to the pathogenicity of the pseudotuberculosis pathogen].
The large pVM82 plasmid isolated from epidemic strains of Yersinia pseudotuberculosis includes the 25MD segment, which encodes a series of properties affecting the virulence of the bacterium. Insertion mutants of pVM82 containing transposition-defective Tn2507 with a kanamycin-resistance marker in different Hind III fragments of the 25MD segment were obtained. By recombination between two homologous pVM82 containing genetic markers in different parts, deletion derivatives of pVM82 plasmid and insertions of the plasmid segment, carrying kanamycin-resistance marker, into a chromosome were obtained. Results were obtained suggesting the presence in the plasmid 25MD segment of a transposon-like structure capable of migrating from pVM82 plasmid onto a chromosome and from a chromosome and pVM82 onto pRP1.2 plasmid of a broad host range.
Il'ina
T S
TS
Fil'kova
S L
SL
D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements; D007613:Kanamycin Resistance / Q000235:genetics; D016254:Mutagenesis, Insertional; D010957:Plasmids; D014774:Virulence / Q000235:genetics; D015011:Yersinia pseudotuberculosis / Q000235:genetics* / Q000472:pathogenicity
D004251:DNA Transposable Elements
Geneticheskoe izuchenie plazmidy pVM82, vnosiashcheĭ vklad v patogennost' vozbuditelia psevdotuberkuleza.