Characterization of the melanogenic system in Vibrio cholerae, ATCC 14035.
The nature of the pigment formed by Vibrio cholerae and the characterization of its biosynthetic pathway is shown. This microorganism is able to synthesize melanin-like pigment when cultured in the presence of L-tyrosine. Other phenolic chemicals related to L-tyrosine do not lead to pigment production. The microorganism has no tyrosine hydroxylase activity, and the levels of dopa oxidase activity are very low, making the existence of a tyrosinase very unlikely. However, Vibrio cholerae contained transaminases that transforms L-tyrosine into p-hydroxyphenylpyruvate. Moreover, Vibrio cholerae is able to go further in the catabolic pathway, releasing a great amount of homogentisic acid. This acid can spontaneously be oxidized to its p-quinone form, which subsequently polymerizes leading to pigment formation. It is concluded that the pigment formed by Vibrio cholerae is not synthesized by the Raper-Mason pathway, but by a L-tyrosine catabolism pathway leading to homogentisic acid. Some simple properties of that melanin are compared to model eu- and pheomelanin, but no clear distinction could be stated, indicating the similarity between all these pigments.
Ruzafa
C
C
Department of Biochemistry, Molecular Biology B, Faculty of Biology, University of Murcia, Spain.
Sanchez-Amat
A
A
Solano
F
F
D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D006713:Homogentisic Acid / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008543:Melanins / Q000096:biosynthesis*; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010667:Phenylpyruvic Acids / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism; D014443:Tyrosine; D014734:Vibrio cholerae / Q000378:metabolism*
D003470:Culture Media; D008543:Melanins; D010667:Phenylpyruvic Acids; C010590:4-hydroxyphenylpyruvic acid; D014443:Tyrosine; D014442:Monophenol Monooxygenase; D000637:Transaminases; D006713:Homogentisic Acid