title: Effect of splenectomy on hepatic metastasis of colon carcinoma and natural killer activity in the liver.
abstract: We have previously demonstrated that administration of killed streptococcal preparation (OK432), a biological modifier, increased the number of asialo GM1-positive cells in the liver, enhanced NK activity of hepatic mononuclear cells, and reduced the number of hepatic metastases of colon 38 adenocarcinoma that were inoculated into the superior mesenteric vein of C57BL/6 strain mice. In the present study, to clarify the role of the spleen in immune surveillance of the liver, the effect of splenectomy on hepatic metastasis of colon carcinoma and on hepatic NK activity has been examined. The number of hepatic metastasis increased in the splenectomized mice, compared with that in sham-operated mice. Administration of OK432 increased the number of asialo GM1-positive cells in the liver and enhanced NK activity of hepatic mononuclear cells in both groups, but NK activity of hepatic mononuclear cells in the splenectomized mice was less than that of the sham-operated mice. An enhanced NK activity of these cells was abolished by treatment with anti-asialo-GM1 antibody plus complement in vitro. Interleukin-2 mRNA expression was increased in the spleen 2 hr after OK432 administration and persisted until 8 hr, but was scarcely noted in the liver. On the other hand, NK activity of hepatic mononuclear cells in the asialo GM1-positive cell-depleted (previous administration of antiserum against asialo GM1) mice was enhanced after OK432 administration in the sham operated and splenectomized mice, but an enhanced NK activity in these mice was only partially or not at all abolished by treatment with anti-asialo GM1 antibody plus complement in vitro, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Internal Medicine (II), Faculty of Medicine, University of Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shiratori
authors.forename: T
authors.initials: T
authors.lastname: Kawase
authors.forename: R
authors.initials: R
authors.lastname: Nakata
authors.forename: M
authors.initials: M
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Hikiba
authors.forename: K
authors.initials: K
authors.lastname: Okano
authors.forename: M
authors.initials: M
authors.lastname: Matsumura
authors.forename: Y
authors.initials: Y
authors.lastname: Niwa
authors.forename: Y
authors.initials: Y
authors.lastname: Komatsu
authors.forename: S
authors.initials: S
authors.lastname: Shiina
mesh_terms: D000818:Animals; D003110:Colonic Neoplasms / Q000473:pathology*; D005677:G(M1) Ganglioside / Q000276:immunology / Q000378:metabolism; D007376:Interleukin-2 / Q000235:genetics; D007694:Killer Cells, Natural / Q000276:immunology*; D008099:Liver / Q000276:immunology* / Q000378:metabolism; D008113:Liver Neoplasms / Q000276:immunology* / Q000556:secondary*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010844:Picibanil / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000276:immunology; D013156:Splenectomy
chemical_list: D007376:Interleukin-2; D012333:RNA, Messenger; D005677:G(M1) Ganglioside; D010844:Picibanil; C018835:asialo GM1 ganglioside