Matrix-assisted laser desorption mass spectrometry for the detection of recombinant bovine growth hormone in sustained-release form.
We employed matrix-assisted laser desorption mass spectrometry (LD-MS) to detect recombinant bovine growth hormone (r-bGH) in sustained-release preparations. After preliminary extraction in phosphate buffer, LD-MS provided a precise determination of the molecular mass (M(r)) of the r-bGH contained in 38 sustained-release preparations. The hormone was characterised using enzyme immunoassay, immunoblotting and amino acid sequencing. Rapid detection is essential for analysing large numbers of samples, and for monitoring the use of r-bGH in zootechnical productions and its administration as a "high-tech" drug for therapeutic purposes.
Borromeo
V
V
Istituto di Fisiologia Veterinaria e Biochimica, Milan, Italy.
Berrini
A
A
Secchi
C
C
Brambilla
G F
GF
Cantafora
A
A
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003692:Delayed-Action Preparations / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D013006:Growth Hormone / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D003692:Delayed-Action Preparations; D011994:Recombinant Proteins; D013006:Growth Hormone