Serum concentration and increased temperature alter Mayaro virus RNA and protein synthesis in Aedes albopictus (mosquito)-infected cells.
We have previously shown the inhibition of Mayaro virus multiplication in Aedes albopictus-infected cells maintained at a supraoptimal temperature for growth (37 degrees C) and a stimulation of virus production in response to high serum concentrations in the incubation medium. In the present study, we addressed the question of how the effect of continuous heat stress and high serum concentration soon after infection interfere with virus macromolecule synthesis. Cells maintained at 28 degrees C in the presence of 2% serum synthesized a viral genomic RNA of 12 kb and a subgenomic RNA of 5.2 kb 6 h postinfection. Analysis of the protein profile showed the presence of the viral nucleocapsid protein of 34 kDa (P34). However, if infected cells were maintained at 37 degrees C, a smear starting immediately below the 5.2-kb RNA was noticed and the viral P34 was not detected by SDS-PAGE. Addition of 10% serum to the growth medium of infected cells maintained at 37 degrees C results in a viral RNA profile and protein synthesis similar to those observed in cultures kept at 28 degrees C, i.e., the smear was not observed and the P34 protein was detected. The results suggest that the inhibition of virus multiplication by temperature may be related to the inhibition of viral nonstructural protein synthesis early during infection. The presence of high serum levels in the incubation medium protects macromolecule synthesis against heat stress.
Motta
M C
MC
Instituto de Biofísica Carlos Chagas Filho, CCS, Universidade Federal do Rio de Janeiro, Brasil.
Fournier
M V
MV
Carvalho
M G
MG
D000330:Aedes / Q000821:virology*; D000524:Alphavirus / Q000254:growth & development / Q000502:physiology*; D000818:Animals; D001769:Blood; D002478:Cells, Cultured; D003470:Culture Media; D016679:Genome, Viral; D012367:RNA, Viral / Q000096:biosynthesis*; D013696:Temperature; D017361:Viral Nonstructural Proteins / Q000502:physiology*; D014779:Virus Replication
D003470:Culture Media; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins