title: A 10-amino-acid linear sequence of VP1 of foot and mouth disease virus containing B- and T-cell epitopes induces protection in mice.
abstract: The area of foot and mouth disease virus (FMDV) comprising residues 140 and 160 of capsid protein VP1 has been used extensively as an immunogen in natural and experimental hosts. A detailed epitope mapping of this region, however, has not been reported. For this purpose a synthetic peptide containing the residues 135 to 160 (p135-160) of VP1 of FMDV O1 Campos was analyzed for its T- and B-cell epitopes. The p135-160 is highly immunogenic, either by itself or coupled to a carrier protein (BSA), elicits a long-lasting neutralizing antibody response in mice, and provides solid protection against virulent challenge. By using a set of synthetic 10mer overlapping peptides, which cover the entire sequence 135-160 of VP1, we have shown that at least four discrete B epitopes are regularly distributed along the peptide. Although immunization with each of the 10mers coupled with BSA as a carrier protein induced peptide-specific antibody responses, individually none of the 10mers was able to induce neutralizing antibodies. However, anti-135-160 antibodies sorted by immunoaffinity chromatography using each of the 10mers revealed the existence of at least four discrete neutralizing sites: one spanning residues 135-144, at least two more between residues 140 and 154, and another in the region 150-160. Moreover, T-cell epitopes were identified, both by antigen-dependent proliferation assays and by adoptive cell transfer. By both methods, a T-cell epitope was located in the area comprising residues 135-144; the cell transfer experiment, which seems to be more sensitive, also identified a second T-cell epitope between residues 150 and 160. Interestingly, when the region 135-144, which contains both B- and T-cell epitopes, was in a tandem repeat configuration it induced a strong neutralizing antibody response in mice and solid protection against the challenge.
authors.affiliation: Instituto de Virologia, C.I.C.V., INTA-Castelar, Pcia, Buenos Aires, Rep. Argentina.
authors.forename: P
authors.initials: P
authors.lastname: Zamorano
authors.forename: A
authors.initials: A
authors.lastname: Wigdorovitz
authors.forename: M
authors.initials: M
authors.lastname: Perez-Filgueira
authors.forename: C
authors.initials: C
authors.lastname: Carrillo
authors.forename: J M
authors.initials: JM
authors.lastname: Escribano
authors.forename: A M
authors.initials: AM
authors.lastname: Sadir
authors.forename: M V
authors.initials: MV
authors.lastname: Borca
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D005537:Aphthovirus / Q000276:immunology*; D002213:Capsid / Q000737:chemistry / Q000276:immunology*; D036022:Capsid Proteins; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control*; D016219:Immunotherapy, Adoptive; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D009842:Oligopeptides / Q000138:chemical synthesis / Q000276:immunology; D012710:Serum Albumin, Bovine; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D018985:Epitopes, B-Lymphocyte; D018984:Epitopes, T-Lymphocyte; D009842:Oligopeptides; C442501:VP1 protein, Foot-and-mouth disease virus; D012710:Serum Albumin, Bovine