title: Quantitative determination of functional thiol groups on intact cell surfaces by resonance Raman spectroscopy.
abstract: The sensitivity and selectivity of resonance Raman spectroscopy, combined with electronic spectroscopy, has been used to develop a method to quantify the membrane thiol population in situ in viable erythrocytes. This technique is based on the thiol-disulfide reaction of Ellman's reagent (5,5'-dithio-bis-2-nitrobenzoic acid). It has the advantage that continuous monitoring of lysis is simple and a correction can be made for any interference resulting from lysis. In addition, the extent of reaction can be expressed as a ratio of the reagent signal, providing an internal calibrant.
authors.affiliation: Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK.
authors.forename: M
authors.initials: M
authors.lastname: O'Donnell
authors.forename: W E
authors.initials: WE
authors.lastname: Smith
authors.forename: A C
authors.initials: AC
authors.lastname: MacCuish
authors.forename: R
authors.initials: R
authors.lastname: Wilson
mesh_terms: D002138:Calibration; D004910:Erythrocyte Membrane / Q000737:chemistry*; D006801:Humans; D013059:Spectrum Analysis, Raman; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D013438:Sulfhydryl Compounds