title: Subtyping of Legionella pneumophila isolates by arbitrarily primed polymerase chain reaction.
abstract: Arbitrarily primed polymerase chain reaction (AP-PCR) was used to differentiate strains of Legionella pneumophila isolated from different water sources in a resort hotel in Benidorm, Alicante, Spain, where an outbreak of Legionnaires' disease occurred among a group of tourists between 65 and 80 years of age. All isolates were L. pneumophila serogroup 1, subtype Pontiac (Knoxville 1). Five different patterns (P1 to P5) were obtained by AP-PCR. The number of bands per pattern varied between 4 and 11. Patterns P1 and P2 represented 60 and 20% of L. pneumophila isolates, respectively. Since different subpopulations of L. pneumophila coexisted (up to three different AP-PCR patterns were identified in a single room), it was not possible to link an individual L. pneumophila strain to the occurrence of this outbreak.
authors.affiliation: Laboratorios Real, C. E. Durviz, S. L., Valencia, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Ledesma
authors.forename: M L
authors.initials: ML
authors.lastname: Camaró
authors.forename: E
authors.initials: E
authors.lastname: Carbonell
authors.forename: T
authors.initials: T
authors.lastname: Sacristán
authors.forename: A
authors.initials: A
authors.lastname: Martí
authors.forename: S
authors.initials: S
authors.lastname: Pellicer
authors.forename: J
authors.initials: J
authors.lastname: Llorca
authors.forename: P
authors.initials: P
authors.lastname: Herrero
authors.forename: M A
authors.initials: MA
authors.lastname: Dasí
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015373:Bacterial Typing Techniques; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D016952:Legionella pneumophila / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D007877:Legionnaires' Disease / Q000453:epidemiology / Q000382:microbiology; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping; D013030:Spain / Q000453:epidemiology; D014871:Water Microbiology; D014881:Water Supply
chemical_list: D000911:Antibodies, Monoclonal; D017931:DNA Primers