Phenytoin inhibits expression of microtubule-associated protein 2 and influences cell-viability and neurite growth of cultured cerebellar granule cells.
The objective of this study was to show whether an in vitro model for Phenytoin-related cytoskeletal impairment could be helpful to investigate cerebellar side effects of Phenytoin (DPH). DPH dose-and time-dependently resulted in decreasing numbers of vital cells. Cells formed only a rarefied intercellular neuritic network. This effect was already evident 24 h after plating. Western-blot analysis revealed that the expression of the dendritic marker microtubule-associated protein 2 (MAP2) was dramatically decreased in DPH-treated cultures.
Kempermann
G
G
Abteilung Neuropathologie des Pathologischen Institutes, Neurozentrum der Universitt, Freiburg i. Br., Germany.
Volk
B
B
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology* / Q000187:drug effects / Q000254:growth & development; D003599:Cytoskeleton / Q000187:drug effects; D003864:Depression, Chemical; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis*; D016501:Neurites / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010672:Phenytoin / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles
D008869:Microtubule-Associated Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D010672:Phenytoin; C022616:thiazolyl blue