[Participation of sialic acid residue in the fibrinogen-fibrin conversion by thrombin].
In order to estimate the effects of sialic acid residues in fibrinogen on the fibrinogen-fibrin conversion by bovine thrombin the Michaelis constant (Km) and maximum velocity (Vmax) were determined. The Km value obtained by the use of intact-fibrinogen was smaller than that of asialo-fibrinogen. This fact suggests that the sialic acid residues affected the formation of the enzyme-substrate complex. It was also found that in comparison with the asialo-fibrinogen, the intact-fibrinogen was significantly influenced in the gel formation time by the ionic strength in the reaction solution.
Akihama
S
S
Department of Biochemistry, Meiji College of Pharmacy, Tokyo, Japan.
Matsuda
Y
Y
Fukase
T
T
Yamanaka
A
A
Okude
M
M
D000818:Animals; D001212:Asialoglycoproteins / Q000494:pharmacology* / Q000502:physiology; D001777:Blood Coagulation / Q000187:drug effects; D001779:Blood Coagulation Factors / Q000494:pharmacology* / Q000502:physiology; D002417:Cattle; D005337:Fibrin / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism* / Q000494:pharmacology* / Q000502:physiology; D006801:Humans; D013917:Thrombin / Q000494:pharmacology* / Q000502:physiology
D001212:Asialoglycoproteins; D001779:Blood Coagulation Factors; C032692:asialofibrinogen; D005337:Fibrin; D005340:Fibrinogen; D013917:Thrombin