title: Analysis of underivatized amino acids by capillary electrophoresis using constant potential amperometric detection.
abstract: A mixture of native (underivatized) amino acids is separated by capillary electrophoresis under alkaline conditions (pH approximately 12) and amperometrically detected with a copper-disk microelectrode. A simple design facilitates capillary-electrode alignment without the need for micropositioning equipment. The limits of detection for the amino acids are in the low microM concentration range, and the signal response is linear over 2-3 orders of magnitude. This procedure is applied to analyze the amino acid hydrolysis products from cytochrome c.
authors.affiliation: Department of Chemistry, State University of New York at Buffalo 14260-3000, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.forename: L A
authors.initials: LA
authors.lastname: Colón
authors.forename: R
authors.initials: R
authors.lastname: Dadoo
authors.forename: R N
authors.initials: RN
authors.lastname: Zare
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D003300:Copper; D003574:Cytochrome c Group / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods*; D006868:Hydrolysis; D008839:Microelectrodes; D010710:Phosphates / Q000737:chemistry; D012972:Sodium Hydroxide / Q000737:chemistry
chemical_list: D000596:Amino Acids; D003574:Cytochrome c Group; D010710:Phosphates; D012972:Sodium Hydroxide; D003300:Copper; C018279:sodium phosphate