Cytokine expression in large cell lymphoma associated with acquired immunodeficiency syndrome.
Cytokine expression was examined by reverse transcriptase-polymerase chain reaction (RT-PCR) in a retrospective sampling of 16 AIDS-associated large cell lymphomas (LCL). IL-6 receptor (IL-6R) and IL-10 expression was detected in a majority of the tumor specimens tested, IL-6 expression was detected in 5 of 16 lymphomas that also expressed IL-6R, suggestive of an autocrine mechanism of disease. A subset of tumor samples described as mixed immunophenotype contained large numbers of infiltrating T lymphocytes and macrophages. Immunoperoxidase staining of a representative tumor of mixed immunophenotype demonstrated the presence of HIV-infected macrophages that also stained with anti-IL-6. This finding suggests that IL-6 produced by nonlymphoid cells may act as a paracrine growth factor for tumor cells that express IL-6R. Although earlier studies of AIDs burkitt's lymphoma cell lines suggested that IL-10 expression required EBV infection, 7 of 12 AIDS LCLs that expressed IL-10 did so in the absence of EBV by EBER in situ hybridization. Because AIDS LCLs frequently express cell surface CD5, we speculate that IL-10 may act as an autocrine or paracrine growth factor for this class of lymphoma. These studies suggest that IL-6 and IL-10 are involved in the pathogenesis of AIDS-associated large cell and mixed immunophenotype lymphoma.
Marsh
J W
JW
Department of Medicine, University of California, San Francisco 94110, USA.
Herndier
B
B
Tsuzuki
A
A
Ng
V L
VL
Shiramizu
B
B
Abbey
N
N
McGrath
M S
MS
D001483:Base Sequence; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology; D016483:Lymphoma, AIDS-Related / Q000150:complications / Q000276:immunology* / Q000821:virology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000150:complications / Q000276:immunology* / Q000821:virology; D008969:Molecular Sequence Data; D012313:RNA / Q000096:biosynthesis; D012367:RNA, Viral / Q000032:analysis; D012189:Retrospective Studies
D016207:Cytokines; D015850:Interleukin-6; D012367:RNA, Viral; D016753:Interleukin-10; D012313:RNA