Molecular cloning of linotte in Drosophila: a novel gene that functions in adults during associative learning.
The linotte (lio) gene was identified in a screen for mutations that disrupted 3 hr memory after olfactory associative learning, without affecting the perception of odors or electroshock. The mutagenesis yielded a transposon-tagged gene disruption, which allowed rapid cloning of genomic DNA. The lio transcription unit was identified via rescue of the lio1 learning/memory defect by induced expression of a lio+ transgene in adults. The perception of odors or electroshock remained normal when the lio+ transgene was expressed in these lio1 flies. Learning/memory remained normal when the lio+ transgene was expressed in wild-type (lio+) flies. The lio gene produces only one transcript, the level of expression of which varies throughout development. Sequence analysis indicates that lio encodes a novel protein.
Bolwig
G M
GM
Cold Spring Harbor Laboratory, New York 11724, USA.
Del Vecchio
M
M
Hannon
G
G
Tully
T
T
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D015342:DNA Probes; D004251:DNA Transposable Elements; D004330:Drosophila / Q000196:embryology / Q000235:genetics* / Q000502:physiology; D029721:Drosophila Proteins; D017344:Genes, Insect; D006358:Hot Temperature; D007814:Larva / Q000378:metabolism; D007858:Learning / Q000502:physiology*; D008568:Memory / Q000502:physiology; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D011679:Pupa / Q000378:metabolism; D020794:Receptor Protein-Tyrosine Kinases; D015183:Restriction Mapping; D012903:Smell
D015342:DNA Probes; D004251:DNA Transposable Elements; D029721:Drosophila Proteins; D011506:Proteins; C096467:pigeon protein, Drosophila; D020794:Receptor Protein-Tyrosine Kinases