Pulmonary hypertension in rats. 1. Role of bromodeoxyuridine-positive mononuclear cells and alveolar macrophages.
The role of activated cells determined by staining with bromodeoxyuridine (BrdU) and the role of alveolar macrophages as a source of cytokines were investigated in monocrotaline (MCT)-induced pulmonary hypertension (PH) in rats. BrdU was mainly incorporated by mononuclear cells; their number increased strikingly prior to the development of PH. Cytokines such as interleukin (IL)-1, IL-6 and tumor necrosis factor were produced during the development of PH. The amount of IL-6 showed significant correlation (p < 0.05) with the number of BrdU-positive cells, which, in turn, correlated with the extent of PH. These results indicate that BrdU-positive cells and cytokines produced by alveolar macrophages may play an important role in the pathogenesis of MCT-induced PH in rats.
Miyata
M
M
Department of Internal Medicine II, Fukushima Medical College, Japan.
Sakuma
F
F
Yoshimura
A
A
Ishikawa
H
H
Nishimaki
T
T
Kasukawa
R
R
D000818:Animals; D001973:Bromodeoxyuridine; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D006976:Hypertension, Pulmonary / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000473:pathology*; D016676:Macrophages, Alveolar / Q000276:immunology / Q000473:pathology*; D008297:Male; D016686:Monocrotaline / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley
D016207:Cytokines; D016686:Monocrotaline; D001973:Bromodeoxyuridine