Binding of vitamin D to low-density-lipoprotein (LDL) and LDL receptor-mediated pathway into cells.
The present study was undertaken to identify serum components other than vitamin D-binding proteins that bind to 1,25(OH)2 D3, and its analog. The binding rate of 1,25(OH)2 D3, 22-oxa-1,25(OH)2D3 (OCT) or 25(OH) D3 to total lipoprotein(TLP) represented 16.7%, 4.65%, and 3.11% of total counts added, respectively. Polyacrylamide gel electrophoresis of the TLP revealed that 1,25(OH)2 D3 and OCT were associated with LDL. The binding studies of OCT-bound LDL to the fibroblasts showed specific pathway to the cells mediated by LDL-receptor. These findings may have important implications in understanding the mechanisms of the diverse biological actions of 1,25(OH)2 D3 and in designing a novel delivery system for vitamin D analogs.
Teramoto
T
T
First Department of Internal Medicine, Teikyo University School of Medicine, Tokyo, Japan.
Endo
K
K
Ikeda
K
K
Kubodera
N
N
Kinoshita
M
M
Yamanaka
M
M
Ogata
E
E
D053299:Apolipoprotein B-100; D001055:Apolipoproteins B / Q000378:metabolism; D002112:Calcifediol / Q000097:blood; D002117:Calcitriol / Q000031:analogs & derivatives / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D008074:Lipoproteins / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood*; D011485:Protein Binding; D011973:Receptors, LDL / Q000502:physiology*; D014807:Vitamin D / Q000097:blood*
D053299:Apolipoprotein B-100; D001055:Apolipoproteins B; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D011973:Receptors, LDL; D014807:Vitamin D; D002117:Calcitriol; C051883:maxacalcitol; D002112:Calcifediol