Chemical techniques employed for the primary structural analysis of proteins and peptides.
This chapter summarises modern microchemical approaches to the purification, identification and primary structure analysis of peptides and proteins. Discussion of high-sensitivity purification methods is restricted to two-dimensional polyacrylamide gel electrophoresis (2-DE) and microbore/capillary column reversed-phase high-performance liquid chromatography (RP-HPLC). Associated techniques are discussed, particularly with respect to analysis of the products with current automated amino acid sequencers and mass spectrometers.
Inglis
A S
AS
Joint Protein Structure Laboratory, Ludwig Institute for Cancer Research (Melbourne Branch) Parkville, Victoria Australia.
Reid
G E
GE
Simpson
R J
RJ
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D017421:Sequence Analysis
D000596:Amino Acids; D010455:Peptides; D011506:Proteins