Synthesis and characterization of (d)NTP derivatives substituted with residues of different photoreagents.
Chemical cross-linking agents having a photoactivable azido group are promising for the study of the spatial organization of biopolymers. We describe here a variety of (d)NTPs derivatives (6a, 6b, 7, 11, 12, 14, and 16) bearing the residues of three different photoreagents containing an aromatic azido group (1a, 2a, and 3a). These conjugates provide a wide choice of instruments to investigate nucleic acid-nucleic acid and nucleic acid-protein interaction. The synthesis of new photoreagent 2a has been also fulfilled. This compound is the most attractive for affinity modification of the nucleic acids.
Wlassoff
W A
WA
Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk.
Dobrikov
M I
MI
Safronov
I V
IV
Dudko
R Y
RY
Bogachev
V S
VS
Kandaurova
V V
VV
Shishkin
G V
GV
Dymshits
G M
GM
Lavrik
O I
OI
D001391:Azo Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000138:chemical synthesis* / Q000737:chemistry; D003854:Deoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010777:Photochemistry; D013053:Spectrophotometry; D013329:Structure-Activity Relationship
D001391:Azo Compounds; D003432:Cross-Linking Reagents; D003854:Deoxyribonucleotides; D007202:Indicators and Reagents