title: Isoproterenol-dependent decrease in oxygen uptake and respiratory enzyme activities in rat myocardial tissue and mitochondria.
abstract: OBJECTIVE
  Myocardial damage induced by isoproterenol is believed to be secondary to increased oxygen demands on the heart. Our objective was to test an additional primary action of isoproterenol on tissue and mitochondrial oxidative metabolism and to compare these effects with the effects of other adrenergic agents in the presence of adrenergic inhibitors.
  
  
  METHODS
  Prospective, dose-response study.
  
  
  METHODS
  Research laboratory at a university hospital.
  
  
  METHODS
  Fifty Sprague-Dawley female rats (200 to 350 g), slightly anesthetized with ether and divided into several groups.
  
  
  METHODS
  In 26 rats, the heart was removed, cut into fine slices (0.5-mm thickness), and placed in an ice-cold buffer. In 22 animals, the hearts were perfused in the Langendorff manner and chopped and processed for mitochondrial studies.
  
  
  RESULTS
  We determined the following: a) the direct "in vitro" effects of isoproterenol and related catecholamines on normal oxygen uptake using myocardial slices; b) rat heart oxygen consumption and mitochondrial oxygen uptake from isolated organs, perfused with isoproterenol; c) measurements of enzyme activities in submitochondrial particles from the same perfused hearts; and d) the direct effects of isoproterenol on normal mitochondria isolated from normal nonperfused hearts. The oxygen uptake was determined polarographically with a Clark-type electrode and enzymatic activities were assayed by spectrophotometric reduction of cytochrome c at 550 nm with different mitochondrial substrates. Isoproterenol (0.01 to 100 nM) decreased the oxygen uptake by the heart slices in a dose-dependent manner. In comparison, epinephrine or norepinephrine per se did not change the parameter. However, with the addition of alpha-adrenergic receptor inhibitors, oxygen uptake decreased to values similar to those values obtained with isoproterenol. Also, mitochondria isolated from hearts perfused with isoproterenol had decreased state 3 respiratory rates (by 50%) and decreased respiratory control ratios (by 30%), without changes in adenosine 5'-diphosphate/oxygen ratios. The respiratory chain enzyme activities were also lowered.
  
  
  CONCLUSIONS
  The data suggest that while isoproterenol increases "in vivo" oxygen uptake by the working rat heart, isoproterenol can simultaneously decrease maximal adenosine 5'-diphosphate-induced mitochondrial oxygen uptake and in vitro myocardial tissue oxygen uptake, probably by modifying the mitochondrial respiratory enzymes. This action could be counteracted by alpha-adrenergic agonist effects.
authors.affiliation: Laboratory of Oxygen Metabolism, University Hospital, School of Medicine, University of Buenos Aires, Argentina.
authors.forename: J J
authors.initials: JJ
authors.lastname: Poderoso
authors.forename: S
authors.initials: S
authors.lastname: Fernandez
authors.forename: M C
authors.initials: MC
authors.lastname: Carreras
authors.forename: C G
authors.initials: CG
authors.lastname: Del Bosco
authors.forename: A
authors.initials: A
authors.lastname: Boveris
mesh_terms: D000818:Animals; D002395:Catecholamines / Q000494:pharmacology; D005260:Female; D007545:Isoproterenol / Q000494:pharmacology*; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000201:enzymology; D009206:Myocardium / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002395:Catecholamines; D009245:NADH Dehydrogenase; D007545:Isoproterenol