Evaluation of serum peptide banding patterns of patients on hemodialysis therapy by discriminant analysis.
The present study was directed towards determining serum protein abnormalities, with emphasis on protein deficits in patients on short- and long-term hemodialysis. Serum protein profiles of healthy controls and chronic and end-stage renal failure patients on hemodialysis were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. A total of 104 distinct peptide bands in the molecular weight range of 23 kDa to 300 kDa were encountered for both healthy and patient populations. The SPSS/PC+ discriminant analysis employing group mean statistics revealed significant protein deficits in the patient population. As many as 72 (69.23%) of the 104 individual peptide bands had lower mean scores for the patients as compared to the healthy subjects. In all, 21 (29.2%) of these peptide bands belonged to the high molecular weight range of 185 kDa to 300 kDa while 13 (18.1%) were located in the low molecular weight range of 23 kDa to 36 kDa. To show that patients and healthy controls can be categorized into their respective groups on the basis of their serum protein profiles, a 100% correct classification was established by group membership prediction analysis. Furthermore, using minimization of Wilk's lambda as the criterion for variable selection, it was found that the 285 kDa, 265 kDa, 250 kDa, 155 kDa, 125 kDa, 110 kDa, 32 kDa, 27.5 kDa, 26 kDa and 23.5 kDa serum peptide bands were the most important in classifying an individual as healthy or diseased.
Vahidy
R
R
Department of Microbiology, University of Karachi, Pakistan.
Zaki
M
M
D000293:Adolescent; D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D002648:Child; D016002:Discriminant Analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood*; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D006435:Renal Dialysis
D001798:Blood Proteins