title: A novel series of amphiphilic imidazolinium compounds for in vitro and in vivo gene delivery.
abstract: We have developed three catioinic amphiphiles based on the structure 1-[2-(acyloxy)ethyl]-2-alkyl(alkenyl)-3-(2-hydroxyethyl)imidazolinium chloride. Although these three compounds differ only in the structure of the hydrophobic acyl chains, they differ greatly in their ability to mediate in vivo and in vitro gene delivery. Moreover, in vitro efficiency is not predictive of in vivo efficiency. The myristoyl form is the most effective compound in vitro, and the oleoyl form is the most effective compound in vivo. The compounds readily form suspensions in aqueous media, both in the pure form and as mixtures with either cholesterol or dioleoylphosphatidylethanolamine. These suspensions can be sonicated to produce smaller particles. Particle size, electron microscopy, and the ability to capture glucose suggest that these lipids form liposomes on suspension in aqueous media. When mixed with plasmid DNA, the lipid particles appear to fuse and form larger particles. Fusion is maximal at the critical DNA:lipid ratio where extensive aggregation and precipitation are observed. Therefore, these compounds behave similarly to other cationic liposome-forming lipids upon interaction with DNA.
authors.affiliation: School of Pharmacy, University of Wisconsin, Madison 53706, USA.
authors.forename: I
authors.initials: I
authors.lastname: Solodin
authors.forename: C S
authors.initials: CS
authors.lastname: Brown
authors.forename: M S
authors.initials: MS
authors.lastname: Bruno
authors.forename: C Y
authors.initials: CY
authors.lastname: Chow
authors.forename: E H
authors.initials: EH
authors.lastname: Jang
authors.forename: R J
authors.initials: RJ
authors.lastname: Debs
authors.forename: T D
authors.initials: TD
authors.lastname: Heath
mesh_terms: D000818:Animals; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D002522:Chlorocebus aethiops; D004247:DNA; D005260:Female; D018014:Gene Transfer Techniques; D005798:Genes, Bacterial; D007093:Imidazoles / Q000138:chemical synthesis* / Q000737:chemistry; D007202:Indicators and Reagents; D007668:Kidney; D008081:Liposomes; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008813:Mice, Inbred ICR; D010957:Plasmids; D013329:Structure-Activity Relationship; D014162:Transfection; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D007093:Imidazoles; D007202:Indicators and Reagents; D008081:Liposomes; D004247:DNA; D008156:Luciferases; D015500:Chloramphenicol O-Acetyltransferase; D001616:beta-Galactosidase