title: Cycloheximide and buthionine sulfoximine prevent induction of genotoxic adaptation by cadmium salt against methyl mercuric chloride in embryonic shoot cells of Hordum vulgare L.
abstract: Presoaked seeds of barley Hordeum vulgare L. pretreated with cycloheximide (CH), 10(-6) M or bythionine sulfoximine (BSO), 10(-4) M, were exposed to methyl mercuric chloride (MMCl), 10(-4) M, with or without prior conditioning with cadmium sulfate (CdSO4), 10(-4) M. Subsequently as the seeds germinated the endpoints measured were mitotic index, cells with mitotic aberrations and micronuclei (MNC) in embryonic shoot cells fixed at 40, 44, 48 and 52 h of recovery. Indicated by the significant reduction (p < or = 0.05) of the yield of cells with aberrations or MNC, the results confirmed that CdSO4-conditioning triggered an adaptive response to MMCl-challenge. Pretreatments of CH and BSO, whereas they potentiated the genotoxicity of MMCl, significantly prevented (p < or = 0.05) the Cd-induced genotoxic adaptation. That underscores a possible involvement of proteins in addition to phytochelatins in the underlaying mechanisms.
authors.affiliation: Department of Botany, Berhampur University, India.
authors.forename: J
authors.initials: J
authors.lastname: Patra
authors.forename: A V
authors.initials: AV
authors.lastname: Subhadra
authors.forename: B B
authors.initials: BB
authors.lastname: Panda
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D019328:Buthionine Sulfoximine; D002104:Cadmium / Q000494:pharmacology*; D019187:Cadmium Compounds; D003513:Cycloheximide / Q000494:pharmacology*; D001467:Hordeum / Q000187:drug effects*; D008717:Methionine Sulfoximine / Q000031:analogs & derivatives* / Q000494:pharmacology; D008767:Methylmercury Compounds / Q000633:toxicity*; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index / Q000187:drug effects; D009152:Mutagenicity Tests; D018520:Plant Shoots / Q000166:cytology; D012639:Seeds / Q000187:drug effects; D013431:Sulfates
chemical_list: D019187:Cadmium Compounds; D008767:Methylmercury Compounds; D013431:Sulfates; D002104:Cadmium; D008717:Methionine Sulfoximine; D019328:Buthionine Sulfoximine; C037123:cadmium sulfate; D003513:Cycloheximide; C004925:methylmercuric chloride