Immunosuppressive activity of angiogenin in comparison with bovine seminal ribonuclease and pancreatic ribonuclease.
Angiogenin, a member of the pancreatic-like ribonuclease family with a special biological action (RISBAses), is a basic protein that induces blood vessel formation. Another member of these special ribonucleases, bovine seminal ribonuclease (BS RNase), displays biological properties, including aspermatogenic, embryotoxic, antitumor and immunosuppressive activities. The effects of two angiogenin preparations tested on the biological activities mentioned above are reported and compared with those of BS RNase and RNase A. In contrast to RNase A, which was ineffective in all biological activities tested, angiogenin suppressed significantly the proliferation of human lymphocytes stimulated by phytohemagglutinin or concanavalin A or by allogenic human lymphocytes (mixed lymphocyte culture). However, angiogenin did not affect the growth of human tumor cell lines, development of cow and mouse embryos and spermatogenicity in mice. On the basis of these results, angiogenin is the first monomeric ribonuclease described so far that displays immunosuppressive activity similar to that of the dimeric BS RNase. The immunosuppressive activity of angiogenin might synergize with the effect on neovascularization of tumor tissues and thus contribute to the development of tumor.
Matousek
J
J
Institute of Animal Physiology and Genetics, Academy of Sciences of Czech Republic, Libĕchov, Czech Republic.
Soucek
J
J
Ríha
J
J
Zankel
T R
TR
Benner
S A
SA
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D005314:Embryonic and Fetal Development / Q000187:drug effects; D004722:Endoribonucleases / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D011247:Pregnancy; D011506:Proteins / Q000494:pharmacology*; D012259:Ribonuclease, Pancreatic / Q000494:pharmacology*; D013091:Spermatogenesis / Q000187:drug effects; D014407:Tumor Cells, Cultured / Q000187:drug effects
D007166:Immunosuppressive Agents; D011506:Proteins; D004722:Endoribonucleases; C046366:angiogenin; C032523:ribonuclease SPL; D012259:Ribonuclease, Pancreatic