Detection of beta-1,2-mannosyltransferase in Candida albicans cells.
A particulate insoluble fraction from Candida albicans J-1012 (serotype A) strain cells was obtained as the residue after extracting a 105,000 x g pellet of cell homogenate with 1% Triton X-100. Incubation of this fraction with a mannopentaose, Man beta 1-->2Man alpha 1-->(2Man alpha 1-->)(2)2Man (alpha beta Man5), in the presence of GDP-mannose followed by high performance liquid chromatography showed the formation of a mannohexaose. Analysis of the product by 1H NMR indicates that alpha beta Man5 was changed to Man beta 1-->2Man beta 1-->2Man alpha 1-->(2Man alpha 1-->)2 2Man (alpha beta Man6). This beta-1,2-mannosyltransferase (ManTase) II activity was completely inhibited by Zn2+ and was not restored by the addition of EDTA. The corresponding enzyme fraction from C. albicans NIH B-792 (serotype B) strain cells, the mannan of which does not possess both the alpha beta Man5 and alpha beta Man6 side chains, also exhibited the same beta-1,2-ManTase II activity.
Suzuki
A
A
Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Miyagi, Japan.
Takata
Y
Y
Oshie
A
A
Tezuka
A
A
Shibata
N
N
Kobayashi
H
H
Okawa
Y
Y
Suzuki
S
S
D002176:Candida albicans / Q000201:enzymology*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D004492:Edetic Acid / Q000494:pharmacology; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000096:biosynthesis; D008362:Mannosides / Q000378:metabolism; D008364:Mannosyltransferases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D008670:Metals / Q000494:pharmacology; D008969:Molecular Sequence Data; D013379:Substrate Specificity
D008351:Mannans; D008362:Mannosides; D008670:Metals; D004492:Edetic Acid; D008364:Mannosyltransferases