[The effect of platelet-activating factor antagonists on early bacteria translocation of rat after burn injury].
30% third degree burn model of Wistar rat was used in this experiment. The animals were divided randomly into three groups (normal control, burn, and platelet-activating factor antagonist treatment). After poured E. coli which labelled with acridine orange into intestine, the rats were killed at 6, 12, 24, and 48 hrs postburn, the bacteria in mesentery lymph node (MLN), liver and pulmonary organisms were cultured and counted, also observed by fluorescent microscopy directly. The results showed that, in PAF antagonist (WEB2170) treatment group, the quantity of bacteria in MLN, liver and lung were decreased significantly (P < 0.001). The labelled bacteria in MLN, liver and lung of burn group were 100%, 80.0%, and 50.0% respectively compared with 40.0%, 30.0%, and 20.0% in treatment group. It is suggested that WEB 2170 could protect the intestine from bacteria translocation after burn injury.
Qin
X
X
Institute of Burn Research, Southwest Hospital, Third Millitary Medical University, Chongqing.
Yu
P
P
Yuan
J
J
D000818:Animals; D001381:Azepines / Q000494:pharmacology*; D018988:Bacterial Translocation / Q000187:drug effects*; D002056:Burns / Q000382:microbiology*; D004926:Escherichia coli / Q000502:physiology*; D004927:Escherichia coli Infections / Q000382:microbiology*; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014230:Triazoles / Q000494:pharmacology*
D001381:Azepines; D010972:Platelet Activating Factor; D014230:Triazoles; C063964:bepafant