Primer extension analysis of Streptococcus mutans promoter structures.
To characterize the promoter structures of Streptococcus mutans genes, primer extension analysis was carried out with 4 genes previously isolated in this laboratory. An analysis of the transcription start sites for the gtfB, gtfC, gtfD and ftf genes revealed that each transcript was initiated at a purine residue. Based on these sites, the -10 and -35 sequences for each transcript were deduced and compared. For all 4 transcripts, the -10, but not the -35, sequences were homologous to the Escherichia coli promoter consensus sequences.
Smorawinska
M
M
Department of Oral Biology, State University of New York, Buffalo 14214-3092, USA.
Kuramitsu
H K
HK
D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D005964:Glucosyltransferases / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000032:analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D017422:Sequence Analysis, DNA / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D013295:Streptococcus mutans / Q000235:genetics*; D014158:Transcription, Genetic
D017931:DNA Primers; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; D005964:Glucosyltransferases