title: Does the sulfhydryl or the adenine moiety of CoA enhance firefly luciferase activity?
abstract: Light production by firefly luciferase is limited by product release resulting in flash kinetics. Several compounds (CoA, PPi, and nucleotides) transform the flash-form of light production into continuous light production. The sulfhydryl group of CoA is required; however, since nucleotides are also active, at least two mechanisms (sites) must exist.
authors.affiliation: Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078-0454, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Ford
authors.forename: L M
authors.initials: LM
authors.lastname: Buck
authors.forename: F R
authors.initials: FR
authors.lastname: Leach
mesh_terms: D000227:Adenine Nucleotides / Q000737:chemistry*; D000818:Animals; D003065:Coenzyme A / Q000737:chemistry*; D001517:Coleoptera / Q000201:enzymology; D004229:Dithiothreitol; D004789:Enzyme Activation; D008156:Luciferases / Q000737:chemistry / Q000378:metabolism*; D008163:Luminescent Measurements; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D000227:Adenine Nucleotides; D013438:Sulfhydryl Compounds; D008156:Luciferases; D003065:Coenzyme A; D004229:Dithiothreitol