Selective removal of immunoglobulin E from rat blood by membrane-immobilized antibody.
We examined the suitability of an immunoaffinity membrane [rabbit IgG specific for rat immunoglobulin E (IgE) immobilized on a cellulose membrane] for removing IgE from rat blood passed through a simple extracorporeal circulatory system. To determine the concentration of IgE in the blood, we also developed a highly sensitive chemiluminescence enzyme immunoassay for rat IgE. The IgE levels in the outlet blood from the immunoaffinity membrane module decreased to 30% of the initial concentration within 30 min.
Adachi
T
T
Hatano Research Institute, Food and Drug Safety Center, Kanagawa, Japan.
Mogi
M
M
Harada
M
M
Kojima
K
K
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D007073:Immunoglobulin E / Q000097:blood* / Q000276:immunology; D008163:Luminescent Measurements; D008954:Models, Biological; D051381:Rats
D007073:Immunoglobulin E