title: Individual time-courses of ECP and EPX during allergen provocation tests in asthmatic children.
abstract: To study the time-course of eosinophil cationic protein (ECP) and eosinophil protein X (EPX) during bronchial allergen provocation, we investigated 32 asthmatic children sensitive to house-dust mites as well as 6 non-atopic young adult controls. In all subjects, allergen challenges were performed with house dust mite extracts of Dermatophagoides pteronys-sinus or Dermatophagoides farinae. Blood samples were taken at regular intervals during the 24-h observation period. The individual time-courses of ECP and EPX revealed different characteristic groups of patterns: (1) an isolated early serum peak of both mediators during or within the first 60 min after provocation (2) an early plus a late peak (3) an isolated late peak 12 h after provocation (4) an isolated late peak 24 h after provocation, and (5) no significant variation during the 24-h observation period. The early peak could be due to short-term changes in eosinophil activation, while late peaks may reflect eosinophil proliferation, recruitment, subsequent priming and enhancing of the propensity to release their proteins. ECP and EPX showed a corresponding parallel time-course in nearly all challenges, with EPX-concentration exceeding that of ECP. There was no correlation between ECP/EPX serum concentrations and clinical parameters such as lung function data. From our results we conclude that the striking groups of time-courses of ECP/EPX serum concentration indicate different uniform patterns of eosinophil activation during allergen challenge-but do not predict clinical outcome of provocation. The role of the eosinophil in early asthmatic reactions remains to be established in further studies.
authors.affiliation: University Children's Hospital (KAVH), Berlin, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Kleinau
authors.forename: B
authors.initials: B
authors.lastname: Niggemann
authors.forename: U
authors.initials: U
authors.lastname: Wahn
mesh_terms: D000293:Adolescent; D000328:Adult; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000097:blood* / Q000276:immunology; D001798:Blood Proteins / Q000493:pharmacokinetics*; D002648:Child; D047091:Eosinophil Granule Proteins; D047208:Eosinophil-Derived Neurotoxin; D005260:Female; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D008297:Male; D009299:Nasal Provocation Tests; D012129:Respiratory Function Tests; D012260:Ribonucleases
chemical_list: D039741:Antigens, Dermatophagoides; D001798:Blood Proteins; D047091:Eosinophil Granule Proteins; D006023:Glycoproteins; D047208:Eosinophil-Derived Neurotoxin; D012260:Ribonucleases