Retroviral-mediated gene transfer in human bone marrow cells growth in continuous perfusion culture vessels.
Hematopoietic stem cell gene therapy holds the promise of being able to treat a variety of inherited and acquired diseases of the hematopoietic stem cell. However, to date, genetic modification of the human hematopoietic stem cell has been relatively inefficient. Here, we report the results of using a bioreactor system to expand hematopoietic cells after a brief retrovirus infection using a high titer, replication defective virus encoding for murine CD18. The retrovirus transduced culture continued to produce genetically modified hematopoietic progenitors for up to 6 weeks, the duration of the culture period. Up to one-third of the long-term culture initiating cell (LTC-IC) are genetically modified by the culture conditions. Murine CD18 can be expressed on the cell surface of up to 20% of the mature cells generated by the culture system, suggesting that clinically significant levels of gene transfer may be occurring. These results demonstrate the feasibility of using continuous perfusion bioreactors as a method of efficiently modifying human hematopoietic stem cells.
Eipers
P G
PG
Department of Internal Medicine, University of Michigan, Ann Arbor 48109-0668, USA.
Krauss
J C
JC
Palsson
B O
BO
Emerson
S G
SG
Todd
R F
RF
Clarke
M F
MF
D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells; D018821:CD18 Antigens / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D003673:Defective Viruses / Q000235:genetics*; D005822:Genetic Vectors; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000821:virology; D006801:Humans; D016169:Lymphocyte Function-Associated Antigen-1 / Q000737:chemistry; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology; D046911:Macromolecular Substances; D008565:Membrane Proteins / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D010477:Perfusion; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D018821:CD18 Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D046911:Macromolecular Substances; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins