title: Gamma delta T cells in rhesus monkeys and their response to simian immunodeficiency virus (SIV) infection.
abstract: Recent reports of the increase in peripheral blood gamma delta T cells in HIV+ patients prompted us to examine the gamma delta T cell system in rhesus monkeys (Macaca mulatta) and the responses of these cells to SIV infection. Our results reveal differences in the gamma delta T cell subset composition and their expression of CD8 in the peripheral blood of monkeys and humans. The outgrowth of simian gamma delta T cells in response to Daudi cells is similar to that in humans, but the exposure to IL-2 stimulates preferentially the simian V delta 1 subset rather than the V gamma 9/V delta 2 subset as found in humans. Upon SIV infection of the monkeys, we observed a transient increase of the percentage of total gamma delta T cell and the V gamma 9 subset. gamma delta T cells from infected animals also express more activation markers such as CD69, CD44 and the memory marker CD45RO. However, they respond to a lesser degree to Daudi or IL-2 stimulation in the outgrowth experiments compared with uninfected animals, although the subset composition of total gamma delta T cells is similar in infected and uninfected animals. The results clearly indicate that gamma delta T cells in rhesus monkeys are influenced by SIV infection. The detailed analysis of the gamma delta T cell response to SIV infection can serve as a model for understanding human gamma delta T cell responses to HIV infections.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison 53706, USA.
authors.forename: Y H
authors.initials: YH
authors.lastname: Gan
authors.forename: C D
authors.initials: CD
authors.lastname: Pauza
authors.forename: M
authors.initials: M
authors.lastname: Malkovsky
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D000945:Antigens, Differentiation, T-Lymphocyte / Q000378:metabolism; D018791:CD4 Lymphocyte Count; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D016130:Immunophenotyping; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D008253:Macaca mulatta; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018960:Hyaluronan Receptors; D037181:Lectins, C-Type; D016692:Receptors, Antigen, T-Cell, gamma-delta; D000099192:CD69 Antigens