High levels of DNA adducts in human colon are associated with colorectal cancer.
Colon cancer is one of the most frequent causes of cancer death in western countries. Epidemiological studies suggest that colorectal cancer can be attributed, at least in part, to carcinogens and mutagens present in the diet and/or the environment. The covalent binding of these xenobiotics or their reactive metabolites to DNA is believed to initiate this chemical carcinogenesis. In the present study, using a 32P-postlabeling method, we investigated DNA adduct levels in control colons from patients without colorectal adenocarcinoma and in nontumoral and tumoral tissues from patients with colorectal adenocarcinoma. Our results show that the DNA adduct level is significantly higher (P < 0.001) in nontumoral than in control or tumoral colon samples. For the first time, we demonstrate in humans that the presence of numerous adducts in colonic mucosa is associated with colorectal cancer, a finding in agreement with the importance of chemical factors in causing this disease; therefore, after confirmation of the link between DNA adducts and colorectal cancer, the measurement of DNA adduct levels in colon samples could constitute a useful approach to the early detection of colorectal cancer.
Pfohl-Leszkowicz
A
A
Ecole Nationale Supérieure Agronomique de Toulouse, Laboratoire de Toxicologie et Sécurié Alimentaire, France.
Grosse
Y
Y
Carrière
V
V
Cugnenc
P H
PH
Berger
A
A
Carnot
F
F
Beaune
P
P
de Waziers
I
I
D000230:Adenocarcinoma / Q000737:chemistry*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D003092:Colitis; D003106:Colon / Q000737:chemistry*; D003110:Colonic Neoplasms / Q000737:chemistry*; D004247:DNA / Q000378:metabolism; D018736:DNA Adducts / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012004:Rectal Neoplasms / Q000737:chemistry*; D012810:Sigmoid Diseases; D015262:Xenobiotics / Q000378:metabolism
D018736:DNA Adducts; D015262:Xenobiotics; D004247:DNA