An improved method for determination of thiocyanate in plasma and urine.
An improved spectrophotometric method is described for the determination of thiocyanate in plasma and urine. Thiocyanate is adsorbed on a weak anion-exchange resin with strong affinity for chaotropic ions, and eluted with perchlorate. Thiocyanate is then chlorinated by hypochlorite and quantified according to the König (J Prakt Chem 1904; 69:105-37) reaction by use of isonicotinic acid and 1,3-dimethyl-barbituric acid. The method affords a simple, rapid and sensitive assay for thiocyanate and has a detection limit of 0.93 mumol/l. At thiocyanate concentrations of 107.1 and 167.4 mumol/l in plasma and urine the within-day CVs were 0.69% and 1.1% respectively, and the total imprecision measured for a period of 65 days was 0.98%. Analytical recoveries were quantitative both with urine and plasma samples.
Lundquist
P
P
Department of Clinical Chemistry, University Hospital, Linköping, Sweden.
Kågedal
B
B
Nilsson
L
L
D001463:Barbiturates; D002852:Chromatography, Ion Exchange; D004396:Coloring Agents; D003486:Cyanides; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006997:Hypochlorous Acid; D007539:Isonicotinic Acids; D010472:Perchlorates; D012680:Sensitivity and Specificity; D017670:Sodium Compounds; D013056:Spectrophotometry, Ultraviolet; D013861:Thiocyanates / Q000097:blood* / Q000652:urine*
D001463:Barbiturates; D004396:Coloring Agents; D003486:Cyanides; D007539:Isonicotinic Acids; D010472:Perchlorates; D017670:Sodium Compounds; D013861:Thiocyanates; D006997:Hypochlorous Acid; C018924:1,3-dimethylbarbituric acid; C031068:sodium perchlorate