Analysis of lysine-dependent yeast sporulation: a decrease in cyclic AMP is not required for initiation of meiosis and sporulation in Saccharomyces cerevisiae.
Cells of the yeast Saccharomyces cerevisiae sporulated in nutrient-rich medium containing L-lysine. Sporulation was specific to the presence of L-lysine and was initiated when the cellular content of this basic amino acid reached approximately 0.2-0.5 mmol (g cells)-1, at early stationary phase. The formation of asci was most efficient at pH 7.0 and 50-100 mM L-lysine; in these optimum conditions, the sporulation frequency reached about 60% after 5 d incubation. The L-lysine-dependent sporulation system in nutrient-rich conditions was distinct from the currently used potassium-acetate-dependent system in nutrient-deficient conditions. Analysis of the L-lysine-dependent system indicated that, prior to entrance into meiosis and/or sporulation processes, the yeast cells change in shape, their pool sizes for L-cysteine and glutathione alter, and they synthesize a protein with a molecular mass of 15 kDa. A low level of cAMP was not required for the entrance into meiosis and/or sporulation.
Suizu
T
T
Research Institute, Gekkeikan Sake, Co., Ltd, Kyoto, Japan.
Tsutsumi
H
H
Kawado
A
A
Inose
T
T
Suginami
K
K
Murata
K
K
D000085:Acetates / Q000494:pharmacology; D019342:Acetic Acid; D000596:Amino Acids / Q000032:analysis; D000242:Cyclic AMP / Q000032:analysis*; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000032:analysis; D006601:Hexoses / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000494:pharmacology*; D008320:Maltose / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*; D013172:Spores, Fungal / Q000187:drug effects / Q000502:physiology
D000085:Acetates; D000596:Amino Acids; D006601:Hexoses; D008320:Maltose; D000242:Cyclic AMP; D005978:Glutathione; D008239:Lysine; D019342:Acetic Acid