title: Characterization of group A streptococci isolated in Kuala Lumpur, Malaysia.
abstract: T-agglutination patterns of 190 strains of group A streptococci isolated between January 1989 and December 1993 from body fluids (10), throat culture (56), pus (51) and skin lesions (73) were determined. Mucoid colonial morphology was exhibited by 6.3% (12/190) of the strains on initial isolation. Type T-5,11,27,44 comprised 23.7%, followed by T-1,3,13,B3264 (11.1%), T-4,6 (8.4%) and T-8,25, Imp 19 (7.9%). About 42% (80/190) strains could not be characterized by T agglutination pattern. T-typing of 71 selected strains at WHO Collaborating Center, Minneapolis yielded similar results. Nineteen selected strains were further characterized by M-typing; only three strains were M-typeable. These strains were isolated from throat (M1), sputum (M5) and pus (M12). About 68% (48/71) isolates produced serum opacity factor. These data support the existence of as yet uncharacterized group A streptococcal serotypes in this region.
authors.affiliation: National Streptococcus Reference Laboratory, Faculty of Medicine, National University of Malaysia.
authors.forename: F
authors.initials: F
authors.lastname: Jamal
authors.forename: S
authors.initials: S
authors.lastname: Pit
authors.forename: D R
authors.initials: DR
authors.lastname: Johnson
authors.forename: E L
authors.initials: EL
authors.lastname: Kaplan
mesh_terms: D000372:Agglutination Tests; D000942:Antigens, Bacterial; D016470:Bacteremia / Q000382:microbiology; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D001826:Body Fluids / Q000382:microbiology; D002352:Carrier Proteins; D006801:Humans; D008296:Malaysia; D010447:Peptide Hydrolases / Q000032:analysis; D010614:Pharynx / Q000382:microbiology; D012703:Serotyping; D012867:Skin / Q000382:microbiology; D013290:Streptococcal Infections / Q000382:microbiology*; D013297:Streptococcus pyogenes / Q000145:classification* / Q000166:cytology / Q000302:isolation & purification; D013492:Suppuration / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C021908:opacity factor; C014689:streptococcal M protein; D010447:Peptide Hydrolases