Inhibition of ICE family proteases by baculovirus antiapoptotic protein p35.
The baculovirus antiapoptotic protein p35 inhibited the proteolytic activity of human interleukin-1 beta converting enzyme (ICE) and three of its homologs in enzymatic assays. Coexpression of p35 prevented the autoproteolytic activation of ICE from its precursor form and blocked ICE-induced apoptosis. Inhibition of enzymatic activity correlated with the cleavage of p35 and the formation of a stable ICE-p35 complex. The ability of p35 to block apoptosis in different pathways and in distantly related organisms suggests a central and conserved role for ICE-like proteases in the induction of apoptosis.
Bump
N J
NJ
BASF Bioresearch Corporation, Worcester, MA 01605, USA.
Hackett
M
M
Hugunin
M
M
Seshagiri
S
S
Brady
K
K
Chen
P
P
Ferenz
C
C
Franklin
S
S
Ghayur
T
T
Li
P
P
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001665:Binding Sites; D001667:Binding, Competitive; D020170:Caspase 1; D002460:Cell Line; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000494:pharmacology; D014162:Transfection; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology
D015853:Cysteine Proteinase Inhibitors; D051034:Inhibitor of Apoptosis Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; C086430:inhibitor of apoptosis, Nucleopolyhedrovirus; D003546:Cysteine Endopeptidases; D020170:Caspase 1