Identification of the human neuronal nicotinic cholinergic alpha 2 receptor locus, (CHRNA2), within an 8p21 mapped locus, by sequence homology with rat DNA.
We have identified a cosmid, at the D8S131 locus, that shows sequence homology with exon 2 of the rat gene for the neuronal nicotinic acetylcholine receptor alpha 2 subunit. A 357-bp sequence surrounding a rare cutter AscI site contains a 152-bp region of homology. The human CHRNA2 gene is therefore positioned at the D8S131 locus, which has been mapped to 8p21.
Wood
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S
Department of Medical Genetics, University of British Columbia, Vancouver, Canada.
Schertzer
M
M
Yaremko
M L
ML
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002898:Chromosomes, Human, Pair 8; D004247:DNA / Q000737:chemistry / Q000235:genetics; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D009474:Neurons / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051381:Rats; D011978:Receptors, Nicotinic / Q000096:biosynthesis* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D011978:Receptors, Nicotinic; D004247:DNA; C078382:endodeoxyribonuclease AscI; D015252:Deoxyribonucleases, Type II Site-Specific