Inhibition of Western-diet induced hyperproliferation and hyperplasia in mouse colon by two sources of calcium.
A Western-style diet containing high-fat and phosphate, and low calcium and vitamin D was fed to mice for 20 weeks. Starting at week 8, subgroups of animals received the Western-style diet supplemented by two different calcium sources: tricalcium phosphate and calcium citrate malate. Hyperproliferation (increased [3H]thymidine-labelled cells/colonic crypt) and hyperplasia (increased total epithelial cells/crypt) developed in the sigmoid colon after 8 weeks of feeding the Western-style diet confirming previous results, and these were reversed at later periods by the addition of the two calcium sources to the Western-style diet. Findings indicate that the modified colonic epithelial cell hyperproliferation and hyperplasia which have been associated with subsequent development of colonic neoplasia, are induced in mice fed a Western-style diet, and the addition of calcium to the diet inhibited their development in the colonic mucosa.
Richter
F
F
Irving Weinstein Laboratory for Gastrointestinal Cancer Prevention, Gastroenterology and Nutrition Service, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Newmark
H L
HL
Richter
A
A
Leung
D
D
Lipkin
M
M
D000818:Animals; D002136:Calcium, Dietary / Q000008:administration & dosage*; D002455:Cell Division; D003106:Colon / Q000473:pathology*; D003110:Colonic Neoplasms / Q000209:etiology; D004041:Dietary Fats / Q000009:adverse effects*; D005260:Female; D006965:Hyperplasia; D051379:Mice; D008810:Mice, Inbred C57BL; D010710:Phosphates / Q000008:administration & dosage / Q000009:adverse effects*; D014807:Vitamin D / Q000008:administration & dosage*
D002136:Calcium, Dietary; D004041:Dietary Fats; D010710:Phosphates; D014807:Vitamin D