title: A base pair between tRNA and 23S rRNA in the peptidyl transferase centre of the ribosome.
abstract: Interaction of the conserved CCA terminus of tRNA with rRNA in the peptidyl transferase P site has been studied by in vitro genetics. A watson-Crick G-C pair between G2252 in a conserved hairpin loop of 23S rRNA and C74 at the acceptor end of tRNA is required for proper functional interaction of the CCA end of tRNA with the ribosomal P site. These findings establish a direct role for 23S rRNA in protein synthesis.
authors.affiliation: Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz 95064, USA.
authors.forename: R R
authors.initials: RR
authors.lastname: Samaha
authors.forename: R
authors.initials: R
authors.lastname: Green
authors.forename: H F
authors.initials: HF
authors.lastname: Noller
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D017124:Conserved Sequence; D004926:Escherichia coli; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009690:Nucleic Acid Conformation; D010458:Peptidyl Transferases / Q000378:metabolism*; D012329:RNA, Bacterial / Q000378:metabolism; D012338:RNA, Ribosomal, 23S / Q000235:genetics / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
chemical_list: D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S; D012343:RNA, Transfer; D010458:Peptidyl Transferases