A CD44 monoclonal antibody differentially regulates CD11a/CD18 binding to intercellular adhesion molecules CD54, CD102 and CD50.
We have made a monoclonal anti-CD44 antibody which is able to activate the leukocyte integrin CD11a/CD18. Activated T cells strongly aggregated, and the aggregation was shown to be intercellular adhesion molecule (ICAM)-1 (CD54) and ICAM-2 (CD102) dependent. Using purified ICAM coated on plastic, only binding to ICAM-1 was increased by the CD44 antibody, whereas activation by phorbol ester increased binding to both ICAM-1 and ICAM-3. The binding to ICAM-2 was not affected by either treatment. These findings show that the CD11a/CD18 integrin can be activated in a ligand-specific manner by engagement of CD44.
Vermot-Desroches
C
C
Department of Biosciences, University of Helsinki, Finland.
Wijdenes
J
J
Valmu
L
L
Roy
C
C
Pigott
R
R
Nortamo
P
P
Gahmberg
C G
CG
D000911:Antibodies, Monoclonal / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D000943:Antigens, Differentiation; D018845:CD11 Antigens / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002449:Cell Aggregation / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D018960:Hyaluronan Receptors / Q000276:immunology*; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D018845:CD11 Antigens; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; C065289:ICAM2 protein, human; C483616:ICAM3 protein, human; D018799:Intercellular Adhesion Molecule-1