title: Expression of cytochrome P450 CYP1B1 in breast cancer.
abstract: The expression of CYP1B1 has been identified in breast cancer using the reverse transcriptase-polymerase chain reaction and immunoblotting. CYP1B1 mRNA was expressed in the majority of breast tumours and immunoblotting of breast tumours identified a single protein band of molecular weight 60 kDa corresponding to the predicted molecular weight of human CYP1B1. This is the first study to identify CYP1B1 expression in a tumour where it may represent a previously unknown pathway for the metabolism of oestradiol and chemotherapeutic drugs.
authors.affiliation: Department of Pathology, University of Aberdeen, UK.
authors.forename: J A
authors.initials: JA
authors.lastname: McKay
authors.forename: W T
authors.initials: WT
authors.lastname: Melvin
authors.forename: A K
authors.initials: AK
authors.lastname: Ah-See
authors.forename: S W
authors.initials: SW
authors.lastname: Ewen
authors.forename: W F
authors.initials: WF
authors.lastname: Greenlee
authors.forename: C B
authors.initials: CB
authors.lastname: Marcus
authors.forename: M D
authors.initials: MD
authors.lastname: Burke
authors.forename: G I
authors.initials: GI
authors.lastname: Murray
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001189:Aryl Hydrocarbon Hydroxylases; D001483:Base Sequence; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D065633:Cytochrome P-450 CYP1B1; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis* / Q000235:genetics; D017931:DNA Primers; D005260:Female; D006801:Humans; D015151:Immunoblotting; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D001189:Aryl Hydrocarbon Hydroxylases; C585496:CYP1B1 protein, human; D065633:Cytochrome P-450 CYP1B1