title: Analysis of peripheral blood lymphocyte subsets, NK cells, and delayed type hypersensitivity skin test in patients with premature ovarian failure.
abstract: OBJECTIVE
  Premature ovarian failure (POF) probably belongs to the group of autoimmune endocrinopathies. Cell-mediated immune parameters were investigated. Sex steroids have a profound effect on the immune system. POF patients and postmenopausal control women (PM) were tested with or without estrogen substitution.
  
  
  METHODS
  A novel FACS analysis system (using double labeling techniques) was used in 30 patients with POF to enumerate the subjects of peripheral blood lymphocytes and NK cells. Eighteen PM women and 30 healthy men and women served as controls. We also tested the delayed type hypersensitivity skin test (DTH) toward Candida in the POF patient group to be informed on their cell-mediated immune function.
  
  
  RESULTS
  The numbers of blood lymphocytes, CD3+, CD4+ and CD8+T cells, were not abnormal in POF patients. However, HLA-DR+T cells were increased in POF patients and in PM women (P < 0.05). These elevated numbers were partially reversible by estrogen substitution. The number of CD19+ cells (B cells) was elevated, whereas CD3-/CD16+/CD56+ cells (NK cells) were decreased in POF patients (P < 0.05), irrespective of estrogen substitution. DTH skin tests toward 0.1% Candidin (0.1 ml intradermal injection) were negative in 11 out of 20 tested POF patients, compared to only 2 out of 10 tested controls (P < 0.05).
  
  
  CONCLUSIONS
  POF patients show numerous immune cell abnormalities. These abnormalities were only partially due to estrogen deficiency. We hypothesize that these abnormalities either lead to ovarian autoimmunity or may have direct effects on the ovarian function.
authors.affiliation: Department of Obstetrics and Gynaecology, Free University Hospital, Amsterdam, The Netherlands.
authors.forename: A
authors.initials: A
authors.lastname: Hoek
authors.forename: Y
authors.initials: Y
authors.lastname: van Kasteren
authors.forename: M
authors.initials: M
authors.lastname: de Haan-Meulman
authors.forename: H
authors.initials: H
authors.lastname: Hooijkaas
authors.forename: J
authors.initials: J
authors.lastname: Schoemaker
authors.forename: H A
authors.initials: HA
authors.lastname: Drexhage
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000097:blood / Q000276:immunology*; D007518:Isoantibodies / Q000097:blood; D007694:Killer Cells, Natural / Q000276:immunology*; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D016649:Primary Ovarian Insufficiency / Q000097:blood / Q000276:immunology*; D012882:Skin Tests
chemical_list: D007518:Isoantibodies