title: Large-cell anaplastic non-Hodgkin's lymphoma originating in donor cells after allogenic bone marrow transplantation.
abstract: Second neoplasms after allogenic bone marrow transplantation (BMT) occur in donor cells; however, host origin generally cannot be excluded. Using fluorescence in situ hybridization (FISH) the origin of the malignant population can be proven indisputably. In a female patient with CD30+ large-cell anaplastic non-Hodgkin's lymphoma (LCAL) after BMT with an HLA-identical brother donor, FISH using anti-CD30 immunocytochemistry in combination with anti-Y- and anti-EBV RNA probes was applied. In pathological lymph nodes the majority of cells were of donor type (Y). CD30-positive cells were Y-positive; these cells were also EBV-positive. Using FISH and immunocytochemistry we have demonstrated convincingly that this, possibly EBV-induced, LCAC originated in donor cells.
authors.affiliation: Department of Internal Medicine, University Hospital Maastricht/University Limburg Maastricht, The Netherlands.
authors.forename: H C
authors.initials: HC
authors.lastname: Schouten
authors.forename: A H
authors.initials: AH
authors.lastname: Hopman
authors.forename: A M
authors.initials: AM
authors.lastname: Haesevoets
authors.forename: J W
authors.initials: JW
authors.lastname: Arends
mesh_terms: D016026:Bone Marrow Transplantation; D005260:Female; D006566:Herpesviridae Infections / Q000150:complications; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D017728:Lymphoma, Large-Cell, Anaplastic / Q000821:virology*; D008875:Middle Aged; D016609:Neoplasms, Second Primary / Q000473:pathology / Q000821:virology*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000628:therapy*; D014184:Transplantation, Homologous; D014412:Tumor Virus Infections / Q000150:complications