Fluorescence drift detection as a novel QC procedure for DNA cell-cycle analysis.
The goal of this work was to develop an objective, quantitative, and reproducible method of detecting fluorescence drift which may have occurred during DNA cell-cycle data acquisition. Quality control software, "TruPloid," is described that analyzes list-mode files to detect and quantify fluorescence drift using three separate statistical tests. We show that fluorescence drift may lead to a variety of measurement artifacts including high coefficients of variation, obscuring of small populations and creation of distinct artificial peaks. Forty percent of 50 archived list-mode files displayed fluorescence drift, which demonstrates the need for detection methods to deal with this source of DNA cell-cycle histogram artifacts.
Seamer
L C
LC
University of New Mexico, Albuquerque, USA.
Altobelli
K K
KK
D016477:Artifacts; D002453:Cell Cycle / Q000235:genetics*; D004247:DNA / Q000032:analysis*; D003627:Data Interpretation, Statistical; D005434:Flow Cytometry / Q000592:standards*; D006801:Humans; D011786:Quality Control; D015203:Reproducibility of Results; D012984:Software
D004247:DNA