title: Ultrastructural localization of Na,K-ATPase in the gerbil cochlea.
abstract: The transport enzyme Na,K-ATPase has been localized to several different cell types within the inner ear by enzyme cytochemistry, immunohistochemistry, and in situ hybridization. Although these histochemical procedures have provided a fairly consistent pattern of the enzyme's distribution, the precise location of Na,K-ATPase in the cell membrane of some polarized and non-polarized cell types remains uncertain. We addressed this problem in the gerbil cochlea using electron microscopic immunogold cytochemistry. The results confirmed prior ultrastructural localization of Na,K-ATPase along the basolateral plasma membrane of strial marginal and outer sulcus epithelial cells but differed from a previous report in failing to detect the enzyme at the surface of strial intermediate cells. The findings also concurred with and extended previous work in showing immunogold labeling along the entire cell membrane of non-polarized Type II fibrocytes in the inferior portion of the spiral ligament and of subpopulations of fibrocytes in the suprastrial and supralimbal regions. Our observations agreed further with light microscopic immunostaining in displaying uniform gold labeling for Na,K-ATPase in the neurilemma of Type I spiral ganglion neurons, even though these cells are completely ensheathed by myelin. Surprisingly, the enzyme was detectable in the neurilemma of afferent but not that of efferent nerve processes beneath hair cells.
authors.affiliation: Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston 29425, USA.
authors.forename: K
authors.initials: K
authors.lastname: Nakazawa
authors.forename: S S
authors.initials: SS
authors.lastname: Spicer
authors.forename: B A
authors.initials: BA
authors.lastname: Schulte
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D003051:Cochlea / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology / Q000648:ultrastructure; D005849:Gerbillinae; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis*
chemical_list: D000254:Sodium-Potassium-Exchanging ATPase