Evaluation of malondialdehyde as an index of lead damage in rat brain homogenates.
Lipid peroxidation in vitro homogenates of brain was examined as sequela of lead toxicity. The levels of malondialdehyde (MDA) in homogenates of rat brain (1 ml, 5% w/v) treated with lead (50 micrograms) alone or in combination with ascorbic acid (100 micrograms), alphatocopherol (100 micrograms) or hydroquinone (100 micrograms) were evaluated. The levels of MDA were consistently evoked by lead in a dose-related manner. The toxicity of lead was further advanced by the action of the pro-oxidant drug ascorbic acid on the brain. However, the anti-oxidant drugs alphatocopherol and hydroquinone decreased the toxic effect of lead on the brain. These results clearly show that the enhanced lipid peroxidation may provide a basis of lead-induced neurotoxicity.
Shafiq-ur-Rehman
Department of Pharmacology, J. N. Medical College, Muslim University, Aligarh, India.
Rehman
S
S
Chandra
O
O
Abdulla
M
M
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004347:Drug Interactions; D006873:Hydroquinones / Q000494:pharmacology; D007854:Lead / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008315:Malondialdehyde / Q000032:analysis*; D051381:Rats; D014810:Vitamin E / Q000494:pharmacology
D006873:Hydroquinones; D014810:Vitamin E; D007854:Lead; D008315:Malondialdehyde; D001205:Ascorbic Acid; C031927:hydroquinone