Deafferentation-induced expression of GAP-43, NCAM, and NILE in the adult rat dorsal horn following pronase injection of the sciatic nerve.
The expression of growth-associated protein 43 (GAP-43), neural cell adhesion molecule (NCAM), and nerve-growth-factor-inducible large external glycoprotein (NILE) in the adult rat dorsal horn was examined at several survival times after unilateral pronase injection of the sciatic nerve. Pronase injection produces a permanent major loss of sciatic primary afferents in the dorsal horn, and there is a later sprouting of saphenous afferents into the sciatic territory. Small-diameter myelinated and nonmyelinated saphenous afferents sprout within the superficial dorsal horn, and larger, myelinated afferents sprout within the deep dorsal horn. In the present study, GAP-43 and NCAM immunoreactivity increased in the superficial dorsal horn by 10 days after injection. By 20 days, the increase spread into the deep dorsal horn; NCAM returned to normal after 1-2 months, but GAP-43 persisted up to 4 months. NILE immunoreactivity appeared in laminae I and II by 10 days and increased up to 30 days; by 2 months no NILE remained. NILE never spread into the deeper dorsal horn, regardless of survival time. These data suggest a correlation in the expression of both NCAM and NILE with the sprouting of fine-diameter sprouting afferents in laminae I and II, and of NCAM expression with the sprouting of larger-diameter afferents in the deep dorsal horn.(ABSTRACT TRUNCATED AT 250 WORDS)
LaMotte
C C
CC
Section of Neurosurgery, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
Kapadia
S E
SE
Arsenault
K
K
Wolfe
M
M
D000344:Afferent Pathways / Q000187:drug effects / Q000502:physiology; D000818:Animals; D005260:Female; D019922:GAP-43 Protein; D005727:Ganglia, Spinal / Q000187:drug effects / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000502:physiology*; D009414:Nerve Growth Factors / Q000502:physiology*; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000502:physiology*; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules / Q000502:physiology*; D017729:Presynaptic Terminals / Q000187:drug effects / Q000502:physiology; D011402:Pronase; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000187:drug effects / Q000502:physiology*
D019922:GAP-43 Protein; D008562:Membrane Glycoproteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules; D011402:Pronase