title: Microvascular reactivity after crystalloid, cold blood, and warm blood cardioplegic arrest.
abstract: BACKGROUND
  The effects of three techniques of cardioplegic arrest on endothelium-dependent microvascular function of the right and left ventricles were examined in a canine model of cardiopulmonary bypass.
  
  
  METHODS
  Oxygenated cold crystalloid cardioplegia and cold blood cardioplegia groups, (n = 11 each) had hypothermic cardiopulmonary bypass (28 degrees C), topical cooling, antegrade arrest, and intermittent antegrade delivery. A warm blood cardioplegia group (n = 11) had normothermic cardiopulmonary bypass (37 degrees C), antegrade arrest, and continuous antegrade delivery. All groups underwent cardioplegic arrest for 1 hour followed by 1 hour of reperfusion. Dogs that did not have instrumentation were used as controls (n = 10). Coronary microvessels (100 to 200 microns in internal diameter) were examined in a pressurized, no-flow state with video microscopic imaging and electronic dimension analysis.
  
  
  RESULTS
  Ischemic arrest with cold crystalloid cardioplegia significantly (p < 0.05) impaired endothelium-dependent relaxations in both ventricles to acetylcholine (left ventricle, 69% +/- 4%, and right ventricle, 73% +/- 5%, versus control left ventricle, 100% +/- 0.3%, and control right ventricle, 100% +/- 0.3%) and the calcium ionophore (left ventricle, 70% +/- 6%, and right ventricle, 68% +/- 3%, versus control left ventricle, 98% +/- 1%, and control right ventricle, 98% +/- 1%). In the cold blood cardioplegia group, endothelium-dependent relaxations to acetylcholine (left ventricle, 96% +/- 1%, and right ventricle, 87% +/- 4%) and the calcium ionophore (left ventricle, 88% +/- 3%, and right ventricle, 78% +/- 7%) were preserved. In the warm blood cardioplegia group, endothelium-dependent responses to acetylcholine (92% +/- 3%) and the calcium ionophore (96% +/- 1%) were preserved in the left ventricle, but the right ventricle showed reduced (p < 0.05) reactivity to the endothelium-dependent acetylcholine (77% +/- 8%) and the calcium ionophore (69% +/- 8%). Endothelium-independent relaxation to sodium nitroprusside was similar to controls in all groups for both ventricles, thus indicating normal smooth muscle responsiveness.
  
  
  CONCLUSIONS
  Cardioplegic arrest with cold blood cardioplegia preserved the endothelium-dependent response in the right and left ventricles, whereas cold crystalloid cardioplegia impairs this response. Warm blood cardioplegia preserved the endothelium-dependent response in the left ventricle, but this response was reduced in the right ventricle. This suggests that blood cardioplegia and hypothermia may be important in protection of microvascular endothelium and that the right ventricle may be more vulnerable to damage than the left ventricle.
authors.affiliation: Department of Surgery, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: C O
authors.initials: CO
authors.lastname: Murphy
authors.lastname: Pan-Chih
authors.forename: J P
authors.initials: JP
authors.lastname: Gott
authors.forename: R A
authors.initials: RA
authors.lastname: Guyton
mesh_terms: D000818:Animals; D003331:Coronary Vessels / Q000502:physiology*; D004285:Dogs; D004727:Endothelium / Q000502:physiology; D006324:Heart Arrest, Induced / Q000379:methods*; D007036:Hypothermia, Induced; D008833:Microcirculation; D013696:Temperature; D016277:Ventricular Function, Left / Q000502:physiology*; D016278:Ventricular Function, Right / Q000502:physiology*