A novel intracellular antigen in HL-60 cells that changes in molecular weight after granulocytic and monocytic differentiation.
The present study reports the identification and partial characterization of a novel antigen with M(r) 100,000 by a monoclonal antibody (D29A8) that was obtained by immunizing BALB/c mice with nuclei of HL-60 cells. D29A8 detected mainly a nucleolar macromolecule with M(r) 100,000 (p100). On the other hand, when HL-60 cells were induced to differentiate either into a granulocytic or monocytic pathway, the antibody detected mainly a cytoplasmic macromolecule with M(r) 95,000 (p95). Since two subtypes of the antigen (p100 and p95) appear to be present in the same cells that differ in the stage of cell differentiation, the antigen may play an important role in cellular differentiation.
Katsura
Y
Y
3rd Department of Internal Medicine, National Defense Medical College, Tokyo, Japan.
Shimomura
S
S
Takatani
O
O
Sato
N
N
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000912:Antibodies, Neoplasm / Q000276:immunology; D000951:Antigens, Neoplasm / Q000737:chemistry*; D002454:Cell Differentiation; D002466:Cell Nucleolus / Q000276:immunology*; D006098:Granulocytes / Q000166:cytology*; D018922:HL-60 Cells / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009000:Monocytes / Q000166:cytology*; D011233:Precipitin Tests
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm