Hypoxia and reoxygenation-induced injury of renal epithelial cells: effect of free radical scavengers.
The aim of this study was to characterize injuries of LLC-PK1 and MDCK cells exposed to hypoxia and reoxygenation. Exposure of LLC-PK1 cells to hypoxia reduced the ATP contents and increased the leakage of lactate dehydrogenase (LDH), but MDCK cells had no such injuries. Hypoxia-reoxygenation of LLC-PK1 cells dramatically increased LDH leakage, which was suppressed by free radical scavengers, N,N'-diphenyl-p-phenylenediamine, superoxide dismutase and N,N'-dimethylthiourea. These results suggest that use of LLC-PK1 cells has advantages for the investigation of ischemia-reperfusion injury of the kidney as an in vitro model and that generation of oxygen radicals is involved in the cellular injury induced by hypoxia-reoxygenation.
Yonehana
T
T
Division of Pharmacology, Osaka University of Pharmaceutical Sciences, Japan.
Gemba
M
M
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D015687:Cell Hypoxia / Q000502:physiology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D007668:Kidney / Q000098:blood supply* / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D018374:LLC-PK1 Cells; D015227:Lipid Peroxidation / Q000187:drug effects; D010100:Oxygen / Q000633:toxicity*; D051381:Rats; D013482:Superoxide Dismutase / Q000494:pharmacology; D013552:Swine; D013890:Thiourea / Q000031:analogs & derivatives / Q000494:pharmacology
D000975:Antioxidants; D016166:Free Radical Scavengers; D000255:Adenosine Triphosphate; C038983:1,3-dimethylthiourea; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; D013890:Thiourea; D010100:Oxygen