Evidence that the antigen receptors of cytotoxic T lymphocytes interact with a common recognition pattern on the H-2Kb molecule.
Recognition of class I MHC antigens involves interaction between TCRs of cytotoxic T lymphocytes (CTL) and the two alpha helices of MHC molecules. Using a combined panel of H-2Kb mutants selected by either a CTL clone or MAbs, we have shown evidence that the TCRs of 59 Kb-specific CTL clones shared a common binding pattern on the H-2Kb molecule. Mutations of amino acid residues at the C-terminal regions, but not the N-terminal regions, of the alpha helices abrogated the recognition by the majority of the clones. The data suggests that TCRs predominantly recognize the class I MHC molecule with an orientation that is parallel to the beta-pleated strands and diagonal to the alpha helices.
Sun
R
R
Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA.
Shepherd
S E
SE
Geier
S S
SS
Thomson
C T
CT
Sheil
J M
JM
Nathenson
S G
SG
D000818:Animals; D002461:Cell Line, Transformed; D003601:Cytotoxicity Tests, Immunologic; D006183:H-2 Antigens / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation / Q000235:genetics; D011948:Receptors, Antigen, T-Cell / Q000276:immunology* / Q000378:metabolism*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism*
D006183:H-2 Antigens; C032876:H-2Kb protein, mouse; D011948:Receptors, Antigen, T-Cell