title: Mechanism of the activation of proteinase inhibitor synthesis by systemin involves beta-sheet structure, a specific DNA-binding protein domain.
abstract: We analyzed a tertiary structure of systemin, the first identified polypeptide plant hormone, using two-dimensional NMR spectroscopy. From these data and molecular dynamics calculations we concluded that the peptide can adopt a Z-like-beta-sheet structure, which has previously been found in many specific DNA-binding proteins. Using DNA-cellulose affinity chromatography, we showed that systemin binds strongly to DNA. We suggest that the specific systemin-DNA interaction, particularly in a promoter region of the proteinase inhibitors, could effect gene expression and thus explain the biological activity of systemin.
authors.affiliation: Max-Planck-Institute for Medical Research, Heidelberg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Slósarek
authors.forename: H R
authors.initials: HR
authors.lastname: Kalbitzer
authors.forename: P
authors.initials: P
authors.lastname: Mucha
authors.forename: P
authors.initials: P
authors.lastname: Rekowski
authors.forename: G
authors.initials: G
authors.lastname: Kupryszewski
authors.forename: M
authors.initials: M
authors.lastname: Giel-Pietraszuk
authors.forename: M
authors.initials: M
authors.lastname: Szymański
authors.forename: J
authors.initials: J
authors.lastname: Barciszewski
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002846:Chromatography, Affinity; D003196:Computer Graphics; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007781:Lactoferrin / Q000378:metabolism; D018551:Solanum lycopersicum / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D011480:Protease Inhibitors / Q000378:metabolism*; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D004268:DNA-Binding Proteins; D010446:Peptide Fragments; D010455:Peptides; D010940:Plant Proteins; D011480:Protease Inhibitors; C073704:systemin; D004247:DNA; D007781:Lactoferrin