Determination of doxycycline in human plasma and urine samples by high performance liquid chromatography. Application for drug monitoring in malaria chemoprophylaxis.
A reverse-phase high performance liquid chromatographic method with ultraviolet detection is described for the measurement of doxycycline in human plasma and urine. After liquid-solid extraction on a Bond Elut C18 cartridge, doxycycline and demeclocycline (internal standard) are separated on a Novapak C18 column by isocratic elution. The mobile phase consists of acetonitrile-oxalate buffer, pH 2.3 (25:75; v/v). The eluent is monitored with an ultraviolet detector at 355 nm. The lower limit of quantification in plasma is close to 25 ng/ml. No chromatographic interference can be detected from endogenous compounds, tetracycline group antibiotics or antimalarial drugs. The method is accurate and precision is good with inter- and intra-assay relative standard deviations lower than 6.7%. The chromatographic procedure takes 8 minutes and can be used for therapeutic drug monitoring, clinical and pharmacokinetic studies.
Prévosto
J M
JM
Laboratoire de biochimie, toxicologie et pharmacologie du professeur Blanchot, hôpital d'instruction des Armées Desgenettes, Lyon, France.
Béraud
B
B
Cheminel
V
V
Gaillard
Y
Y
Mounier
C
C
Chaulet
J F
JF
D000900:Anti-Bacterial Agents / Q000097:blood / Q000627:therapeutic use / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004318:Doxycycline / Q000097:blood* / Q000627:therapeutic use / Q000652:urine*; D005500:Follow-Up Studies; D006801:Humans; D008288:Malaria / Q000517:prevention & control*
D000900:Anti-Bacterial Agents; D004318:Doxycycline