title: Pancreatic metaplasia in Barrett's esophagus. An immunohistochemical study.
abstract: While pancreatic metaplasia has been observed in gastric mucosa of patients with chronic gastritis, it has not been described in ectopic gastric mucosa. We have identified focal clusters of cells resembling pancreatic acinar cells (CPACs) in 11 of 350 biopsies of Barrett's mucosa from 120 patients with Barrett's esophagus enrolled in a clinical efficacy trial of omeprazole versus ranitidine for treatment of gastroesophageal reflux disease. Three additional cases from our surgical files were also studied. Immunoreactivity for trypsin and chymotrypsin was present in the CPACs of all 14 cases, while stains for alpha-amylase and lipase were each positive in 12 of 13. A few cells in the CPACs were also positive for chomogranins (12 of 13 cases), serotonin (seven of 13 cases), somatostatin (three of 12), gastrin (four of 11), and pancreatic polypeptide (two of 13). No staining was seen for insulin or glucagon. Ultrastructural studies performed in one case showed features of pancreatic exocrine and endocrine (PP-type) cells in cells within CPACs. These results collectively indicate that the CPACs are aggregates of true pancreatic acinar cells admixed with a few endocrine cells. This pancreatic parenchyma in Barrett's mucosa is most likely of metaplastic origin and could be derived from the transitional zone cells or from pluripotent stem cells in the esophageal mucosa or from metaplasia of mucus cells. While the development of pancreatic metaplasia in Barrett's esophagus appears to be unrelated to drug therapy, the clinical relevance of this distinctive histological finding needs further investigation.
authors.affiliation: New England Medical Center, Department of Pathology, Boston, Massachusetts 02111, USA.
authors.forename: S
authors.initials: S
authors.lastname: Krishnamurthy
authors.forename: Y
authors.initials: Y
authors.lastname: Dayal
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001471:Barrett Esophagus / Q000378:metabolism / Q000473:pathology*; D002864:Chromogranins / Q000032:analysis; D002918:Chymotrypsin / Q000032:analysis; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008049:Lipase / Q000032:analysis; D008297:Male; D008679:Metaplasia; D008875:Middle Aged; D010179:Pancreas / Q000737:chemistry / Q000473:pathology*; D010191:Pancreatic Polypeptide / Q000032:analysis; D014357:Trypsin / Q000032:analysis; D000516:alpha-Amylases / Q000032:analysis
chemical_list: D002864:Chromogranins; D010191:Pancreatic Polypeptide; D008049:Lipase; D000516:alpha-Amylases; D002918:Chymotrypsin; D014357:Trypsin