title: Lack of a synergistic effect between estradiol and dihydrotestosterone in the masculinization of the zebra finch song system.
abstract: Previous studies have suggested that both major active metabolites of testosterone, estradiol (E2) and dihydrotestosterone (DHT), are needed for complete masculinization of the brain regions that control song in passerine birds. However, DHT treatment of hatchling female zebra finches has only small masculinizing effects on the song system. To assess whether E2 and DHT have a synergistic effect on the masculinization of the zebra finch song system, female zebra finches were given Silastic implants of E2 on the day of hatching (day 1) either without any additional hormone treatment or in combination with DHT on days 1, 14, or 70. At 105 to 110 days of age, we measured the volumes of Area X, higher vocal center (HVC), robust nucleus of the archistriatum (RA), soma sizes in HVC, RA, and the lateral magnocellular nucleus of the neostriatum (IMAN), and neuron density and number in RA. E2 masculinized all of the measures in the song system with the exception of the number of neurons in RA. DHT did not synergize with E2 to produce any additional masculinization of the attributes measured. These data demonstrate that the combination of E2 and DHT did not result in the complete masculinization of the song control nuclei and argue against the importance of androgen in sexual differentiation of the song system.
authors.affiliation: Program in Neuroscience, University of California, Los Angeles 90095-1527, USA.
authors.forename: E C
authors.initials: EC
authors.lastname: Jacobs
authors.forename: W
authors.initials: W
authors.lastname: Grisham
authors.forename: A P
authors.initials: AP
authors.lastname: Arnold
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001717:Birds / Q000502:physiology*; D002452:Cell Count / Q000187:drug effects; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004357:Drug Synergism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D009474:Neurons / Q000187:drug effects; D012727:Sex Characteristics; D014770:Virilism / Q000503:physiopathology*; D014828:Vocalization, Animal / Q000187:drug effects*
chemical_list: D013196:Dihydrotestosterone; D004958:Estradiol