Adhesion-inducible DNA synthesis regulated by tyrosine phosphorylation/dephosphorylation.
Non-malignant rat liver epithelial cell line BRL was reported to adhere to a substrate by fibronectin. DNA synthesis of these cells was induced by adhesion to a substrate by fibronectin, while DNA synthesis in non-adhered cells was not observed. These results indicated that DNA synthesis in BRL cells is inducible by the cell adhesion signaling. Adhesion-inducible DNA synthesis was strongly inhibited by Herbimycin A. In contrast, vanadate showed the tendency to promote adhesion-inducible DNA synthesis. These results suggest that DNA synthesis caused by the cell adhesion in these cells was regulated by both phosphorylation and dephosphorylation at tyrosine residue.
Mashima
K
K
Department of Science, Rikkyo (St. Paul's) University Nishi-Ikeburkuro, Tokyo, Japan.
Furuya
Y
Y
D000818:Animals; D016227:Benzoquinones; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004848:Epithelium / Q000378:metabolism; D005353:Fibronectins; D047029:Lactams, Macrocyclic; D008099:Liver / Q000378:metabolism; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D011809:Quinones / Q000494:pharmacology; D051381:Rats; D017828:Rifabutin / Q000031:analogs & derivatives; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism*; D014638:Vanadates / Q000494:pharmacology
D016227:Benzoquinones; D004791:Enzyme Inhibitors; D005353:Fibronectins; D047029:Lactams, Macrocyclic; D011809:Quinones; D017828:Rifabutin; D019000:Phosphotyrosine; D014638:Vanadates; D014443:Tyrosine; C020754:herbimycin; D004247:DNA; D011505:Protein-Tyrosine Kinases