[The study on the mechanism of EGF inducing the in vitro meiotic maturation resumption of mouse oocytes].
The effect and mechanism of EGF and progesterone on the meiotic maturation resumption were studied. The results showed that progesterone regulated the action of EGF. The inhibitor of 3 beta-HSD, the key enzyme of progesterone synthesis, Epostane could not only prevent the effect of EGF, but inhibit the progesterone production promoted by EGF in cultured ovary granulosa cells as well. On the other hand, heparin could lessen the function of EGF and progesterone, which implies that calcium participated the effect of EGF and progesterone. Fura-2 was used to indicate the changes of intracellular calcium level in single cumulus cell. It is found that a calcium wave appeared in cumulus cell when EGF or progesterone acted. But Ca2+ level returned to normal immediately after progesterone was added, and Ca2+ in the cumulus cell still remained higher level after EGF acted. This results indicated that calcium in cumulus cell adjusted the action of EGF and progesterone.
Li
C J
CJ
Jiangsu Academy of Agriculture Science, Nanjing.
Wang
B
B
Fan
B Q
BQ
Wang
J X
JX
Zhang
W N
WN
Zhang
Z X
ZX
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D006133:Growth Substances / Q000494:pharmacology*; D008540:Meiosis / Q000187:drug effects*; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*; D011247:Pregnancy; D011374:Progesterone / Q000096:biosynthesis
D006133:Growth Substances; D011374:Progesterone; D004815:Epidermal Growth Factor; D002118:Calcium