Characterization of multiple prohormone convertase PC1/3 transcripts in porcine ovary.
Overlapping cDNAs encoding porcine prohormone convertase, PC1/3, have been isolated from a pregnant sow ovary cDNA library using a mouse PC1/3 cDNA as a probe. Nucleotide sequence analysis of these cDNAs predicts a PC1/3 precursor protein of 753 amino acid residues, which shares an overall sequence homology of 96, 92, and 92% with the human, rat, and mouse counterparts, respectively. Furthermore, five different polyadenylation sites have been observed. The utilization of these polyadenylation sites results in a length difference of 40-440 bp in the 3' untranslated regions of the transcripts.
Dai
G
G
Department of Obstetrics and Gynecology, Albert Einstein Medical Center, Philadelphia, PA 19141-3098, USA.
Smeekens
S P
SP
Steiner
D F
DF
McMurtry
J P
JP
Kwok
S C
SC
D000595:Amino Acid Sequence; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D001483:Base Sequence; D001665:Binding Sites; D015342:DNA Probes; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D005260:Female; D008969:Molecular Sequence Data; D010053:Ovary / Q000201:enzymology*; D011247:Pregnancy; D045664:Proprotein Convertase 1; D043484:Proprotein Convertases; D017386:Sequence Homology, Amino Acid; D013552:Swine
D015342:DNA Probes; D018076:DNA, Complementary; D043484:Proprotein Convertases; C473656:Pcsk1 protein, mouse; D045664:Proprotein Convertase 1; D016282:Aspartic Acid Endopeptidases