title: HIV type 1 grown on interferon gamma-treated U937 cells shows selective increase in virion-associated intercellular adhesion molecule 1 and HLA-DR and enhanced infectivity for CD4-negative cells.
abstract: Cellular adhesion molecules, such as ICAM-1, -2, and -3; LFA-1; and HLA class I and II are incorporated into HIV-1 virions during budding from infected cells. These virion-associated molecules can be involved in the adsorption to susceptible cells displaying the corresponding counterligands. A number of cytokines have been shown to upregulate the cellular expression of adhesion molecules, such as ICAM-1 and HLA-DR. In this study we investigated the effects of IFN-gamma on the incorporation of ICAM-1, LFA-1, and HLA-DR into mature HIV-1 progeny from chronically infected cells. The ability of such virus progeny to infect either CD4-positive or -negative cells was also investigated. The results indicate that IFN-gamma stimulates the expression of ICAM-1 and of HLA-DR on HIV-1-infected cells, whereas LFA-1 expression is unaffected. The same modifications were also observed on virus progeny, because specific MAbs to ICAM-1 and HLA-DR captured infectious HIV-1 from IFN-treated cells with higher efficiency as compared to virus from control cells, whereas virus binding to anti LFA-1 MAb was unchanged. Moreover, the HIV-1 progeny released from IFN-treated cells showed an increased ability to bind to and to infect CD4-negative cells, whereas the infectivity was basically unchanged for CD4-positive cells. Our results suggest that cytokines, as well as other soluble factors, may expand the host cell range of HIV-1, possibly through modifications of the cell-derived surface molecules on the virions.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Institute of Virology, University La Sapienza, Rome.
authors.forename: C
authors.initials: C
authors.lastname: Castilletti
authors.forename: M R
authors.initials: MR
authors.lastname: Capobianchi
authors.forename: S
authors.initials: S
authors.lastname: Fais
authors.forename: I
authors.initials: I
authors.lastname: Abbate
authors.forename: B
authors.initials: B
authors.lastname: Ficociello
authors.forename: F
authors.initials: F
authors.lastname: Ameglio
authors.forename: P
authors.initials: P
authors.lastname: Cordiali Fei
authors.forename: S M
authors.initials: SM
authors.lastname: Santini
authors.forename: F
authors.initials: F
authors.lastname: Dianzani
mesh_terms: D015704:CD4 Antigens; D002460:Cell Line; D015497:HIV-1 / Q000378:metabolism / Q000472:pathogenicity*; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism / Q000821:virology
chemical_list: D015704:CD4 Antigens; D006684:HLA-DR Antigens; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma