title: Characterization and functional dissection of the galectin-1 gene promoter.
abstract: The galectin-1 gene encodes a beta-galactoside-binding protein whose overexpression is associated with neoplastic transformation and loss of differentiation. Transient transfection assays of a series of deletions constructs (pGAT) showed that the galectin-1 promoter is highly active in cells both expressing and non-expressing the endogenous gene, and that the basal activity is determined by sequences encompassing the transcription start site (-50/+50). Both an upstream (-50/-26) and a downstream position-dependent (+10/+50) cis-elements are necessary for efficient transcriptional activity and are able to bind nuclear proteins.
authors.affiliation: Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Università degli Studi di Napoli Federico II, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Salvatore
authors.forename: C
authors.initials: C
authors.lastname: Contursi
authors.forename: G
authors.initials: G
authors.lastname: Benvenuto
authors.forename: C B
authors.initials: CB
authors.lastname: Bruni
authors.forename: L
authors.initials: L
authors.lastname: Chiariotti
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D037483:Galectin 1; D015870:Gene Expression; D006388:Hemagglutinins / Q000096:biosynthesis* / Q000235:genetics*; D037102:Lectins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D017384:Sequence Deletion; D014162:Transfection
chemical_list: D037483:Galectin 1; D006388:Hemagglutinins; D037102:Lectins; D011994:Recombinant Proteins; D015500:Chloramphenicol O-Acetyltransferase