Normal human cells at confluence get heat resistance by efficient accumulation of hsp72 in nucleus.
We investigated the heat-sensitivity of normal human cells and cells derived from human cancers (cancer cells) heated at 43 degrees C under different culture conditions. Primary human cells which keep in contact with each other show heat resistance, but cancer cells do not. This does not correlate with the total cellular amount of heat shock protein 72, but rather with accumulation of the protein in the nucleus after heating.
Watanabe
M
M
Department of Health Sciences, Faculty of Pharmaceutical Sciences, Nagasaki University, Japan.
Suzuki
K
K
Kodama
S
S
Sugahara
T
T
D015153:Blotting, Western; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002470:Cell Survival; D004622:Embryo, Mammalian; D050884:HSP72 Heat-Shock Proteins; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000032:analysis / Q000096:biosynthesis*; D006358:Hot Temperature; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D014407:Tumor Cells, Cultured
D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins