Structure of prejunctional receptor binding analog of human neuropeptide Y dimer ANA-NPY.
Human neuropeptide Y (NPY) analog ANA-NPY or [L17, Q19, A20, A23, L28, L31]NPY (13-36)-amide binds weakly to postjunctional receptors to raise blood pressure but binds tightly to prejunctional receptors to inhibit neurotransmitter release. ANA-NPY forms a well-conserved anti-parallel helical structure overlapping E23-Y36 with strong amphipathic character. The C-terminal portions of the monomers are better defined than the N-terminal ends. The N-terminal helices extend only from D16-E23/L24. The prejunctional receptor-specific binding site is confined within the C-terminal helices while the residues responsible for partial binding to the postjunctional receptors are located in the more disordered N-terminal segments.
Barden
J A
JA
Department of Anatomy and Histology, University of Sydney, NSW, Australia.
D000595:Amino Acid Sequence; D001665:Binding Sites; D006801:Humans; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D017433:Protein Structure, Secondary; D017476:Receptors, Neuropeptide Y / Q000378:metabolism*
D046911:Macromolecular Substances; D009478:Neuropeptide Y; D010446:Peptide Fragments; D017476:Receptors, Neuropeptide Y; C094733:neuropeptide Y(13-36)amide, N-acetyl-(Leu(17,28,31)Gln(19)Ala(20,23))-