Effect of sulfated xylans during the interaction of [125I]-thrombin with antithrombin III or heparin cofactor II of human plasma.
[125I]-Labeled thrombin was incubated with human plasma and its interactions with the two plasma protease inhibitors antithrombin III (AT-III) or heparin cofactor II (HC-II) were investigated in the presence of oat spelts xylan sulfate (OSXS), sodium pentosan polysulfate (SP-54), and the results were compared with heparin and dermatan sulfate. Addition of OSXS or SP-54 enhanced the complexation of thrombin with HC-II or with both AT-III and HC-II depending upon the concentration and the duration of the interactions of the sulfated compounds with plasma. During the 30 s interaction, OSXS and SP-54 enhanced both AT-III-thrombin and HC-II-thrombin interaction while heparin was more selective and enhanced only the AT-III-thrombin interaction. However after a 2 min interaction, heparin showed an increase in the HC-II-thrombin interaction at higher concentrations. The complexations of AT-III-thrombin and HC-II-thrombin were reversed in the presence of 200 micrograms/ml of SP-54 during a 30 s interaction or in presence of 100 micrograms/ml of OSXS during a 2 min interaction. The Western blotting method of detecting thrombin showed that during the 10 s interaction, heparin enhanced the thrombin-AT-III complex formation while OSXS enhanced the thrombin-HC-II complex formation.
Simmons
R B
RB
Chemistry Department, Prairie View A&M University, Texas, USA.
Newton
G R
GR
Doctor
V M
VM
D000990:Antithrombin III / Q000378:metabolism*; D015153:Blotting, Western; D003871:Dermatan Sulfate / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006493:Heparin / Q000494:pharmacology; D015844:Heparin Cofactor II / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D010426:Pentosan Sulfuric Polyester / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate; D013431:Sulfates / Q000494:pharmacology; D013917:Thrombin / Q000378:metabolism*; D014990:Xylans / Q000494:pharmacology*
D007457:Iodine Radioisotopes; D013431:Sulfates; D014990:Xylans; D003871:Dermatan Sulfate; D012967:Sodium Dodecyl Sulfate; D010426:Pentosan Sulfuric Polyester; D015844:Heparin Cofactor II; D000990:Antithrombin III; D006493:Heparin; D013917:Thrombin