Germline transmission of exogenous genes in chickens using helper-free ecotropic avian leukosis virus-based vectors.
We have used vectors derived from avian leukosis viruses to transduce exogenous genes into early somatic stem cells of chicken embryos. The ecotropic helper cell line, Isolde, was used to generate stocks of NL-B vector carrying the Neo(r) selectable marker and the Escherichia coli lacZ gene. Microinjection of the NL-B vector directly beneath unincubated chicken embryo blastoderms resulted in infection of germline stem cells. One of the 16 male birds hatched (6.25%) from the injected embryos contained vector DNA sequences in its semen. Vector sequences were transmitted to G1 progeny at a frequency of 2.7%. Neo(r) and lacZ genes were transcribed in vitro in chicken embryo fibroblast cultures from transgenic embryos of the G2 progeny.
Thoraval
P
P
Laboratoire de Virologie et d'Oncologie Aviaire, INRA, Nouzilly, France.
Afanassieff
M
M
Cosset
F L
FL
Lasserre
F
F
Verdier
G
G
Coudert
F
F
Dambrine
G
G
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics*; D001354:Avian Leukosis Virus / Q000235:genetics*; D001483:Base Sequence; D001947:Breeding; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D004274:DNA, Recombinant / Q000032:analysis / Q000097:blood; D004279:DNA, Viral / Q000032:analysis / Q000097:blood; D005260:Female; D005347:Fibroblasts; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D019868:Kanamycin Kinase; D008297:Male; D008845:Microinjections; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D012661:Semen / Q000737:chemistry; D013234:Stem Cells; D019076:Transgenes / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
D004274:DNA, Recombinant; D004279:DNA, Viral; D017853:Phosphotransferases (Alcohol Group Acceptor); D019868:Kanamycin Kinase; D001616:beta-Galactosidase