Instability at the H-ras minisatellite is associated with the spontaneous abortion of the embryo.
Recently we have shown that microsatellite instability (MI) is a detectable phenomenon in aborted embryonic tissues. In the present study we investigated if instability is also detectable in a minisatellite located at the 3'-end of the H-ras proto-oncogene, affecting either the repetition number of the 28-bp core generating larger or smaller alleles or its sequence creating a detectable restriction fragment length polymorphism (RFLP). Among 30 aborted embryonic tissues, alterations at the repetition number of the core were found in 3 (10%) while point mutations were detected in 7 (23%) cases. These results indicate that structural alterations of the H-ras minisatellite may be associated with the rejection of the embryo.
Kiaris
H
H
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.
Ergazaki
M
M
Spandidos
D A
DA
D000022:Abortion, Spontaneous / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D004276:DNA, Satellite / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D004622:Embryo, Mammalian; D005260:Female; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D000090063:Proto-Oncogene Mas; D011519:Proto-Oncogenes; D012091:Repetitive Sequences, Nucleic Acid
D017931:DNA Primers; D004276:DNA, Satellite; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific