title: Lack of effect of hormonal treatment and smoking on cytokeratin expression in buccal mucosa.
abstract: Differences in cytokeratin expression of clinically normal buccal mucosa were studied in 50 healthy women by indirect immunofluorescence staining with monoclonal antibodies. The subjects were divided into four groups: control group (N = 18), smokers (N = 8), oral contraceptive users (N = 8) and smokers receiving oral contraceptives (N = 16). Our findings indicate that cytokeratin expression in noncornified stratified epithelium is not influenced by smoking or oestradiol/progesterone treatment. Only cytokeratin No. 19 showed variable patterns of expression but the differences could not be ascribed to smoking or contraceptives. Cytokeratin No. 19 gave a positive reaction in the basal and suprabasal layers in 34 subjects (68%). In 9 (18%) specimens, the staining was positive in the basal cells and showed a positive heterogeneous cytoplasmic reaction in the suprabasal cells. Interestingly, cytokeratin No. 7 was recognized in all epithelial cells except the basal cells. Our results suggest that changes in the serum oestradiol levels do not affect the cytokeratin pattern in buccal mucosa.
authors.affiliation: Department of Oral Pathology, University of Turku, Finland.
authors.forename: R
authors.initials: R
authors.lastname: Leimola-Virtanen
authors.forename: S
authors.initials: S
authors.lastname: Syrjänen
authors.forename: R P
authors.initials: RP
authors.lastname: Happonen
authors.forename: I
authors.initials: I
authors.lastname: Virtanen
authors.forename: M
authors.initials: M
authors.lastname: Luomanen
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal; D003276:Contraceptives, Oral / Q000097:blood / Q000494:pharmacology*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004958:Estradiol / Q000097:blood / Q000494:pharmacology*; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes; D015870:Gene Expression; D006801:Humans; D007633:Keratins / Q000032:analysis* / Q000145:classification / Q000235:genetics; D009061:Mouth Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D011374:Progesterone / Q000494:pharmacology*; D012907:Smoking / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013194:Staining and Labeling
chemical_list: D000911:Antibodies, Monoclonal; D003276:Contraceptives, Oral; D005456:Fluorescent Dyes; D011374:Progesterone; D004958:Estradiol; D007633:Keratins; D016650:Fluorescein-5-isothiocyanate