Spatial synaptic integration in Purkinje cell dendrites.
Synaptic integration occurs within a framework of synaptic connections, and cell type-specific, intrinsic and transmitter-gated ion channels. These components are differentially distributed over the somato-dendritic membrane. Recent results from Purkinje cells and pyramidal cells exemplify some of these mechanisms of spatial synaptic integration. This paper focusses on the cerebellar Purkinje cell. In these neurons, the amplitude and distribution of single climbing fibre and parallel fibre EPSP-evoked Ca2+ influx were regulated by the transient outward, IA-like current in the distal (spiny) dendrites. The synaptically evoked Ca2+ influx was graded from a local response involving only a few terminal spiny dendrites to a propagated Ca2+ spike. The climbing fibre-evoked Ca2+ influx in the spiny dendrites was finely graded by parallel fibre-induced depolarization. Climbing fibre and parallel fibre-evoked Ca2+ influx elicited a short lasting afterhyperpolarization that affected subsequent dendritic Ca2+ influx. In addition, inhibitory synaptic input controlled dendritic Ca2+ influx. Interaction between information from different sources along the dendrites is thus controlled by intrinsic potassium conductances and IPSPs. Different electrophysiological properties are found in the cerebellar neurons. Thus, Golgi cells, stellate cells and granule cells seem to integrate on a shorter intrinsic timescale than do Purkinje cells, the output neuron of the cerebellar cortex. The specific mechanisms by which different types of presynaptic neurons specifically innervate a given dendritic compartment remain to be elucidated, but recent results provide some experimental evidence of a differential distribution of cell adhesion molecules between the axonal and the somato-dendritic membrane, suggesting one mechanism contributing to the ordered distribution of synapses during synaptogenesis.
Midtgaard
J
J
Department of Medical Physiology, University of Copenhagen, Denmark.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003712:Dendrites / Q000502:physiology* / Q000648:ultrastructure; D005071:Evoked Potentials / Q000187:drug effects / Q000502:physiology; D005110:Extracellular Space / Q000502:physiology; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology; D009412:Nerve Fibers / Q000502:physiology; D018408:Patch-Clamp Techniques; D011689:Purkinje Cells / Q000502:physiology* / Q000648:ultrastructure; D013569:Synapses / Q000502:physiology; D014426:Turtles / Q000502:physiology*
D007473:Ion Channels; D002118:Calcium