Comparative study of bone marrow induced by purified BMP and recombinant human BMP-2.
Into a calf muscle pouch in Wistar rats, 50 micrograms purified bone morphogenetic protein (pBMP) or 50 micrograms recombinant human bone morphogenetic protein-2 (rhBMP-2) was implanted using atelopeptide type I collagen solution (CL) as a carrier. Three weeks later bone and bone marrow were induced in both groups. These induced bone and bone marrow were studied histologically. In the pBMP+CL group (n = 5), rich bone matrix and little bone marrow were observed. There was no fatty marrow or angioid tissue observed. In the rhBMP-2+CL group (n = 5), bone matrix and rich marrow including fatty marrow and angioid tissue were observed around and among the bony trabeculae. It was suggested that a "self-supporting bone organ" was induced.
Kusumoto
K
K
Department of Plastic and Reconstructive Surgery, Kansai Medical University, Osaka.
Bessho
K
K
Fujimura
K
K
Konishi
Y
Y
Ogawa
Y
Y
Iizuka
T
T
D000818:Animals; D001853:Bone Marrow / Q000033:anatomy & histology / Q000187:drug effects* / Q000502:physiology*; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000033:anatomy & histology / Q000187:drug effects; D004343:Drug Implants; D006133:Growth Substances / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D008297:Male; D009132:Muscles; D011506:Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology
D019485:Bone Morphogenetic Proteins; D004343:Drug Implants; D006133:Growth Substances; D011506:Proteins; D011994:Recombinant Proteins