Effects of peptide T derivatives on the proliferation of cultured human keratinocytes.
[D-Ala1]peptideT-amide, the linear hexapeptide H-Thr-Hse-Asn-Tyr-Thr-Asp-OH (LPT) and its cyclic analog, cyclo(-Thr-Hse-Asn-Tyr-Thr-Asp-) (CPT), were tested for their effects on the proliferation of cultured normal human keratinocytes (KTs) in comparison with vasoactive intestinal peptide (VIP). [D-Ala1]PT-NH2, LPT and VIP (all 0.1 mumol/l) increased the cell number in KT cultures, whereas CPT was ineffective. The VIP antagonist [N-Ac-Tyr1,D-Phe2]GRF (1-29)-NH2 significantly inhibited the VIP effects on KTs. On the other hand this antagonist did not affect the peptide T (PT) compounds-induced stimulation of KTs, providing indirect evidence that the mitogenic effects of VIP and PT peptides are probably mediated via different receptors.
Marastoni
M
M
Dipartimento di Scienze Farmaceutiche dell' Università di Ferrara, Italy.
Scaranari
V
V
Fantini
F
F
Sevignani
C
C
Tomatis
R
R
D000241:Adenosine / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D015603:Keratinocytes / Q000187:drug effects*; D008969:Molecular Sequence Data; D015717:Peptide T / Q000031:analogs & derivatives* / Q000494:pharmacology*; D051381:Rats; D013268:Stimulation, Chemical; D014660:Vasoactive Intestinal Peptide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D007328:Insulin; D015717:Peptide T; D014660:Vasoactive Intestinal Peptide; D014700:Verapamil; D000241:Adenosine