The nuclear membrane-associated honeycomb structure of the unicellular organism Amoeba proteus: on the search for homologies with the nuclear lamina of metazoa.
In the protozoon Amoeba proteus, a complex and highly organized structure with the morphology of a honeycomb is associated with the nucleoplasmic surface of the nuclear membrane. We have tested whether this structure exhibits similarity to the nuclear lamina of metazoic organisms. First, we have shown that the honeycomb layer is composed of 3 to 5 nm thick protein fibrils resistant to treatment with detergent, high salt, and digestion with nucleases, thus possessing properties typical for karyoskeletal elements. However, in contrast to the meshwork of lamin filaments in somatic cells of metazoic organisms, the honeycomb layer is not tightly anchored to the nucleoplasmic side of pore complexes, or to the inner nuclear membrane. Second, in microinjection experiments we investigated whether fluorescently labeled lamins of Xenopus laevis (lamins A and LI) and Drosophila melanogaster (lamin Dmo) were able to associate in vivo with the Amoeba proteus honeycomb structure. In microinjected amoeba these three lamins were efficiently transported into the nucleus, but did not associate with the nuclear envelope. Our results suggest that the Amoeba proteus nuclear envelope, including the honeycomb layer, does not contain proteins exhibiting high homologies to lamins of metazoan species thus preventing the localized assembly of microinjected lamins along the nuclear periphery.
Schmidt
M
M
Division of Electron Microscopy, Theodor Boveri Institute, University of Würzburg, Germany.
Grossmann
U
U
Krohne
G
G
D016475:3T3 Cells; D000656:Amoeba / Q000648:ultrastructure*; D000818:Animals; D003851:Deoxyribonucleases / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D004330:Drosophila; D019084:Fluorescent Antibody Technique, Indirect; D034882:Lamins; D051379:Mice; D008845:Microinjections; D008856:Microscopy, Fluorescence; D009685:Nuclear Envelope / Q000187:drug effects / Q000648:ultrastructure*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D012260:Ribonucleases / Q000494:pharmacology; D014981:Xenopus
D003902:Detergents; D034882:Lamins; D009687:Nuclear Proteins; D003851:Deoxyribonucleases; D012260:Ribonucleases