Purification and properties of storage proteins (vicilins) from cowpea (Vigna unguiculata) seeds which are susceptible or resistant to the bruchid beetle Callosobruchus maculatus.
Vicilins (7S storage proteins) from seeds of cowpea (Vigna unguiculata) cultivars which are susceptible or resistant to the bruchid beetle C. maculatus were purified by size-exclusion and ion-exchange chromatography. The vicilins were partially characterized by polyacrylamide gel electrophoresis under both denaturing and nondenaturing conditions, by Western blotting and by amino acid analysis. The variant vicilins from C. maculatus-resistant seeds do not differ appreciably from vicilins from susceptible seeds by these criteria except that they are more strongly bound to DEAE-Sepharose, suggesting differences in charge between the various molecules.
Macedo
M L
ML
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
Fernandes
K V
KV
Sales
M P
MP
Xavier-Filho
J
J
D000596:Amino Acids / Q000737:chemistry; D000818:Animals; D015153:Blotting, Western; D001517:Coleoptera / Q000502:physiology*; D004591:Electrophoresis, Polyacrylamide Gel; D015394:Molecular Structure; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification*; D055314:Seed Storage Proteins; D012639:Seeds / Q000737:chemistry*
D000596:Amino Acids; D010940:Plant Proteins; D055314:Seed Storage Proteins; C015575:vicilin protein, plant