Alternative splicing of AMPA receptor subunits: regulation in clonal cell lines.
Alternative splicing of AMPA receptors was investigated in rat PC12 and human SH-SY5Y cells. PC12 cells predominantly expressed GluR-B flip mRNA before and after differentiation. In SH-SY5Y cells, each AMPA-receptor subunit showed a distinct splice variant expression profile throughout differentiation. GluR-B mRNA was comparable in expression levels for flip and flop splice variants. In the other AMPA-receptor subunit transcripts the flip form was the more prominent splice variant. After three days post induction a transient elevation of GluR-B and -D flop mRNA expression could be observed.
Christnacher
A
A
Sandoz Pharma Ltd., Preclinical Research, Basle, Switzerland.
Sommer
B
B
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002460:Cell Line; D002999:Clone Cells; D017931:DNA Primers; D014644:Genetic Variation; D006801:Humans; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D016716:PC12 Cells; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D018091:Receptors, AMPA / Q000096:biosynthesis*
D017931:DNA Primers; D046911:Macromolecular Substances; D012333:RNA, Messenger; D018091:Receptors, AMPA