Increased immunoreactive rat lung ICAM-1 in oleic acid-induced lung injury.
Levels of immunoreactive ICAM-1 in rat lung were followed during the kinetic development of acute oleic acid-induced lung injury in the rat by the ELISA assay. Significant increases in ICAM-1 immunoreactivity were found on rat lung membranes within 30 min of oleic acid injection. The increased immunoreactive ICAM-1 persisted for the duration of the study (4 h) and paralleled lung injury as measured by decreased lung compliance. Enhanced ICAM-1 immunofluorescence was also observed on cryostat sections of lungs from oleic acid-treated rats. No direct effect of oleic acid on ICAM-1 levels of cultured human umbilical vein endothelial cells or rat lung microvascular endothelial cells was observed. This suggests that either oleic acid raises rat lung ICAM-1 levels on endothelial cells by an indirect mechanism or that oleic acid increases ICAM-1 levels on other cell types, such as fibroblasts or lung epithelial cells, by direct or indirect mechanisms. Some of the increased ICAM-1 may also be due to the accumulation of ICAM-1 containing circulating leukocytes in the lung. The role of ICAM-1 in the pathophysiology of oleic acid-induced lung injury and the mechanism by which oleic acid increases ICAM-1 expression in the lung therefore remain to be defined by future experimentation.
Syrbu
S
S
Department of Pharmacology, University of Connecticut Health Center, Farmington 06030, USA.
Thrall
R S
RS
Wisniecki
P
P
Lifchez
S
S
Smilowitz
H M
HM
D000818:Animals; D000911:Antibodies, Monoclonal; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D007700:Kinetics; D008168:Lung / Q000187:drug effects* / Q000378:metabolism*; D008170:Lung Compliance / Q000187:drug effects; D055370:Lung Injury; D008297:Male; D019301:Oleic Acid; D009829:Oleic Acids / Q000633:toxicity*; D051381:Rats; D011916:Rats, Inbred F344
D000911:Antibodies, Monoclonal; D009829:Oleic Acids; D018799:Intercellular Adhesion Molecule-1; D019301:Oleic Acid