[Muscle relaxants in the morphometric study of the respiratory muscles in human beings].
The morphological examination of respiratory muscle can be affected by muscular contraction following biopsy. Most morphometric studies of respiratory muscles, however, have been carried out without taking into account this factor, the effect of which can be reduced by using relaxants when taking samples. Objective. To examine the effect of using a relaxant in the morphometric analysis of muscle fibers. We examined 31 muscle samples from 7 patients. Immediately after removal, each pipe was divided in half. One was placed in an isotonic physiological solution and the other in a solution of curare 0.02%. Later, both samples were processed for morphometric study with ATP-ase, NADTH and PAS tincture. Morphological data recorded for the different types of fibers included measurement of minimum diameter (Dmin), atrophy and hypertrophy indices (AI and HI) and heterogeneity of distribution (SDDmin). The Dmin was smaller in fibers transported in a curare solution than in those transported in physiological solution (67 +/- 2 microns vs. 71 +/- microns, p < 0.05). The same was true of SDDmin (13 +/- 3 vs. 12 +/- 3, p < 0.05), HI (300 +/- 88 vs. 457 +/- 107, p < 0.05). Likewise, we found a similar direct correlation between size of fibers processed with physiological solution and those processed in curare (Dmin, r = 0.731, p < 0.001; HI, r = 0.827, p < 0.001; SDDmin, r = 0.636, p < 0.0001). The use of relaxants in processing muscle samples prevents contraction and should be used systematically in the morphological analysis of muscle fibers.(ABSTRACT TRUNCATED AT 250 WORDS)
Aguar
M C
MC
Servicio de Neumología, Hospital del Mar, Barcelona.
Gea
J
J
Orozco-Levi
M
M
Corominas
J
J
Pastó
M
M
Broquetas
J M
JM
D001706:Biopsy; D003472:Curare / Q000494:pharmacology*; D006652:Histological Techniques; D006801:Humans; D066298:In Vitro Techniques; D008875:Middle Aged; D003473:Neuromuscular Nondepolarizing Agents / Q000494:pharmacology*; D012132:Respiratory Muscles / Q000187:drug effects* / Q000473:pathology; D018709:Statistics, Nonparametric
D003473:Neuromuscular Nondepolarizing Agents; D003472:Curare
Relajantes musculares en el estudio morfométrico de los músculos respiratorios en seres humanos.