title: Distribution of mRNA encoding the inwardly rectifying K+ channel, BIR1 in rat tissues.
abstract: The distribution of mRNA encoding the inwardly rectifying K+ channel, BIR1 [1] was investigated in rat tissues, and a comparison made with the expression of related genes rcKATP and GIRK1 using the reverse transcription-polymerase chain reaction (RT-PCR). This showed BIR1 to be expressed in all areas of the brain examined, in the eye but not in any other peripheral tissue. This pattern was distinct from rcKATP and GIRK1. Additional in situ hybridisation studies of the central expression of BIR1 demonstrated high levels of BIR1 mRNA in the hippocampus, dentate gyrus, taenia tecta and cerebellum and at lower levels in the cortex, habenular nucleus, olfactory bulb, primary olfactory cortex, thalamus, pontine nucleus and amygdaloid nucleus.
authors.affiliation: Human Genetics Group, Sanger Centre, Hinxton, Cambs, UK.
authors.forename: A K
authors.initials: AK
authors.lastname: Dixon
authors.forename: A K
authors.initials: AK
authors.lastname: Gubitz
authors.forename: M L
authors.initials: ML
authors.lastname: Ashford
authors.forename: P J
authors.initials: PJ
authors.lastname: Richardson
authors.forename: T C
authors.initials: TC
authors.lastname: Freeman
mesh_terms: D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D017931:DNA Primers; D015342:DNA Probes; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015221:Potassium Channels / Q000235:genetics* / Q000378:metabolism; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D051676:G Protein-Coupled Inwardly-Rectifying Potassium Channels; C497070:Kcnj6 protein, rat; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger