High-affinity RNA-binding domains of alfalfa mosaic virus coat protein are not required for coat protein-mediated resistance.
A virus-based vector was used for the transient expression of the alfalfa mosaic virus coat protein (CP) gene in protoplasts and plants. The accumulation of wild-type CP conferred strong protection against subsequent alfalfa mosaic virus infection, enabling the efficacy of CP mutants to be determined without developing transgenic plants. Expression of the CP mRNA alone without CP accumulation conferred weaker protection against infection. The activity of the N-terminal mutant CPs in protection did not correlate with their activities in genome activation. The activity of a C-terminal mutant suggested that encapsidation did not have a role in protection. Our results indicate that interaction of the CP with alfalfa mosaic virus RNA is not important in protection, thereby leaving open the possibility that interactions with host factors lead to protection.
Yusibov
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Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 49706, USA.
Loesch-Fries
L S
LS
D017794:Alfalfa mosaic virus / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics / Q000494:pharmacology*; D036022:Capsid Proteins; D004252:DNA Mutational Analysis; D008969:Molecular Sequence Data; D010935:Plant Diseases; D010947:Plants, Toxic; D011523:Protoplasts / Q000821:virology; D012367:RNA, Viral / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014026:Nicotiana / Q000821:virology; D014774:Virulence / Q000187:drug effects
D036022:Capsid Proteins; D012367:RNA, Viral; D016601:RNA-Binding Proteins; C095691:coat protein, Alfalfa mosaic virus