title: Studies on the agronomic characteristics, yield, and saikosaponin content of two Bupleurum species in Taiwan.
abstract: Two Bupleurum species (Bupleurums spp.), i.e., B. falcatum L. cv. Tainung No. 1 and B. kaoi Liu, Chao, et Chuang, were compared for their differences in seed germination, plant characteristics and root yield, and saikosaponin content. Experimental data showed that the most suitable temperature for seed germination of the two species was 16 degrees C. Two treatments, cold stratification at 4 degrees C for 8 weeks and presoaking by running water for 2 days, resulted in higher germination rates. Tainung No. 1 possessed a higher 1,000-seed weight than B. kaoi. However, field survival rate was higher for B. kaoi than for Tainung No.1. Results from field experiments also revealed that stem diameter, leaf width and fresh weight of various plant parts except the root were superior for Tainung No. 1 to B. kaoi. On the contrary, tiller number and root diameter and weight of B. kaoi were higher than those of Tainung No. 1. Harvest data and elevation had significant effects on the agronomic performance of the two Bupleurum spp. Measurements of most traits of the 6 month-old plants were superior to those of the 3 and 10 month-old plants. Cultivation at higher elevation (850 m) favored the development of leaf weight and root length, while cultivation at lower elevation (85 m) facilitated the development of plant height, root diameter, and root weight. Analysis of saikosaponin concentration in the root tissue revealed that average contents of 3.19 and 3.80 mg/g, respectively, for plants grown at the elevations of 850 m and 85 m. Comparison between the two species showed no significant difference in saikosaponin content, ranging from 3.45 to 3.55 mg/g.
authors.affiliation: Department of Agronomy, Taiwan Agricultural Research Institute, Taichung.
authors.forename: S Y
authors.initials: SY
authors.lastname: Liu
authors.forename: W K
authors.initials: WK
authors.lastname: Wu
authors.forename: J Y
authors.initials: JY
authors.lastname: Wang
authors.forename: L M
authors.initials: LM
authors.lastname: Son
authors.forename: M H
authors.initials: MH
authors.lastname: Yen
authors.forename: C C
authors.initials: CC
authors.lastname: Lin
mesh_terms: D029972:Bupleurum; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018525:Germination; D007166:Immunosuppressive Agents; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots; D012502:Sapogenins; D012503:Saponins; D012639:Seeds; D013997:Time Factors
chemical_list: C051274:Bupleurum root extract; D004365:Drugs, Chinese Herbal; D007166:Immunosuppressive Agents; D010936:Plant Extracts; D012502:Sapogenins; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D