The complete sequence of a cucumber mosaic virus from Ixora that is deficient in the replication of satellite RNAs.
A cucumber mosaic virus (CMV-Ix) from Ixora is unusual in that it does not support the accumulation of some well-characterized CMV satellite RNAs in plants. CMV-Ix can support a particular satellite RNA variant which causes lethal tomato necrosis when inoculated with other CMV strains but not when inoculated with CMV-Ix. This difference in ability to support accumulation of specific satellite variants is apparent even when their sequences differ by only 10 nucleotides. Electroporation of tomato protoplasts with combinations of CMV-Ix or CMV-1 RNA plus the same satellite variants showed similar differences in accumulation, indicating a defect in satellite RNA replication and not movement or encapsidation. Pseudorecombinant virus infections between CMV-1 and CMV-Ix indicated that the genomic determinants responsible for this phenotype reside on RNA 1 since only combinations with CMV-Ix RNA 1 failed to replicate satellite RNA. The complete genome of CMV-Ix was cloned, sequenced and compared with the genomes of other cucumoviruses. CMV-Ix is most similar in RNA and protein sequence to subgroup 1 CMV-Fny and CMV-Y but slightly less similar than they are to each other. CMV-Ix and all cucumovirus strains sequenced thus far share a domain in the 3' untranslated portion of their genomic RNAs in which 39 of 40 bases are completely conserved.
McGarvey
P
P
Molecular Plant Pathology Laboratory, PSI, USDA, Beltsville, MD 20705, USA.
Tousignant
M
M
Geletka
L
L
Cellini
F
F
Kaper
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017799:Cucumovirus / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary; D018551:Solanum lycopersicum; D008969:Molecular Sequence Data; D011523:Protoplasts; D012313:RNA / Q000096:biosynthesis*; D019244:RNA, Satellite; D012367:RNA, Viral / Q000096:biosynthesis*; D017386:Sequence Homology, Amino Acid; D014779:Virus Replication
D017931:DNA Primers; D018076:DNA, Complementary; D019244:RNA, Satellite; D012367:RNA, Viral; D012313:RNA