title: The drug resistance-related protein LRP is the human major vault protein.
abstract: Multidrug-resistant cancer cells frequently overexpress the 110-kD LRP protein (originally named Lung Resistance-related Protein). LRP overexpression has been found to predict a poor response to chemotherapy in acute myeloid leukaemia and ovarian carcinoma. We describe the cloning and chromosome localization of the gene coding for this novel protein. The deduced LRP amino acid sequence shows 87.7% identity with the 104-kD rat major vault protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. The LRP gene is located on chromosome 16, close to the genes coding for multidrug resistance-associated protein and protein kinase C-beta, and may mediate drug resistance, perhaps via a transport process.
authors.affiliation: Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
authors.forename: G L
authors.initials: GL
authors.lastname: Scheffer
authors.forename: P L
authors.initials: PL
authors.lastname: Wijngaard
authors.forename: M J
authors.initials: MJ
authors.lastname: Flens
authors.forename: M A
authors.initials: MA
authors.lastname: Izquierdo
authors.forename: M L
authors.initials: ML
authors.lastname: Slovak
authors.forename: H M
authors.initials: HM
authors.lastname: Pinedo
authors.forename: C J
authors.initials: CJ
authors.lastname: Meijer
authors.forename: H C
authors.initials: HC
authors.lastname: Clevers
authors.forename: R J
authors.initials: RJ
authors.lastname: Scheper
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002885:Chromosomes, Human, Pair 16; D018076:DNA, Complementary / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D015388:Organelles / Q000737:chemistry; D051381:Rats; D012261:Ribonucleoproteins / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014407:Tumor Cells, Cultured; D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein
chemical_list: D018076:DNA, Complementary; D009363:Neoplasm Proteins; D012261:Ribonucleoproteins; D020394:Vault Ribonucleoprotein Particles; D000099187:Major Vault Protein