Assignment of the uteroferrin gene (ACP5) to swine chromosome 2q12-->q21 by fluorescence in situ hybridization.
A cosmid clone containing the uteroferrin gene (ACP5) was selected from a cosmid library of swine genomic fragments by colony hybridization using uteroferrin cDNA as a probe. The genomic fragment thus cloned was examined by Southern blot and sequence analysis which demonstrated that it contained at least a part of the uteroferrin gene. The cosmid clone DNA was labeled with biotin, and used a probe for in situ hybridization to swine chromosomes. Hybridization was visualized by the FITC-labeled streptavidin/biotinylated antistreptavidin system together with R-banding of chromosomes. The hybridization signals revealed that the uteroferrin gene (ACP5) is located on swine chromosome 2q12-->q21.
Yasue
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H
Animal Genome Research Group, National Institute of Animal Industry, Ibaraki, Japan.
Kusumoto
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Mikami
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D000135:Acid Phosphatase; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D017404:In Situ Hybridization, Fluorescence; D007527:Isoenzymes; D008667:Metalloproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D013552:Swine / Q000235:genetics*; D000071681:Tartrate-Resistant Acid Phosphatase
D018076:DNA, Complementary; D007527:Isoenzymes; D008667:Metalloproteins; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase