Mammalian artificial chromosomes: a new tool for gene therapy.
Effective therapy by in vivo delivery of DNA requires efficient delivery, long-term maintenance of the DNA that is delivered and physiological levels of expression of the therapeutic gene. Full levels of physiologically controlled expression can be obtained after transfer of intact genes on fragments of DNA hundreds of kilobases in size, as has been demonstrated by the transfer of yeast artificial chromosomes into transgenic mice. Long-term maintenance of input DNA could be achieved if the DNA carried replication origins, a centromere and telomeres to allow maintenance and segregation in mammalian cells, and there has been recent progress towards cloning these elements. These features could be combined as a mammalian artificial chromosome which would confer full levels of controlled expression as well as being maintained in any cell into which it was introduced. Methods which would allow delivery of such large fragments of DNA include liposomes and receptor-mediated uptake, both of which have been shown to work in vivo, making such large constructs potentially applicable for use in gene therapy.
Huxley
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Department of Biochemistry and Molecular Genetics, St Mary's Hospital Medical School, London, UK.
D000818:Animals; D002503:Centromere / Q000235:genetics; D002875:Chromosomes / Q000235:genetics*; D004261:DNA Replication; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D016615:Telomere / Q000235:genetics; D014780:Viruses / Q000235:genetics
D004274:DNA, Recombinant