title: Usage of beta 1 integrin ligands by B cells is developmentally regulated in avian bursa.
abstract: Chicken B cell development takes place in a separate organ, the bursa of Fabricius, which provides the blood-borne stem cells, a microenvironment specialized for B cell maturation. Therefore, chicken can be used as a model to study specifically the molecules and interactions which control the development of the B cell compartment. In this work, we studied expression, localization and function of beta 1 integrins on maturing B cells and bursal stroma. The expression of beta 1 integrins on B cells increases during the embryonic development and beta 1 integrin-positive cells can be found both in the medulla and the cortex throughout the bursal development. The binding assays show that the attachment of B cells to stroma is mediated by beta 1 integrins. Binding to 10-day-old embryonic stroma is fibronectin-independent, whereas fibronectin-mediated binding takes place in an increasing manner during further embryonic maturation. After hatching fibronectin appears to be the main binding site for B cells. However, the epitope of beta 1 integrin which takes part in the adhesion of B cells to stroma and to fibronectin is not crucial for the homing of cells into the bursa in a cell transfer model. Our results indicate that the interaction of beta 1 integrins with their ligands is developmentally regulated in the bursa and suggest that B cell maturation may be partially controlled by this interaction.
authors.affiliation: Department of Medical Microbiology, Turku University, Finland.
authors.forename: E
authors.initials: E
authors.lastname: Palojoki
authors.forename: S
authors.initials: S
authors.lastname: Jalkanen
authors.forename: P
authors.initials: P
authors.lastname: Toivanen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002060:Bursa of Fabricius / Q000196:embryology / Q000276:immunology*; D002478:Cells, Cultured; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D019012:Integrin beta1 / Q000032:analysis / Q000378:metabolism*; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D017154:Stromal Cells / Q000378:metabolism
chemical_list: D019012:Integrin beta1; D008024:Ligands