title: The skeletal effects of spaceflight in growing rats: tissue-specific alterations in mRNA levels for TGF-beta.
abstract: Dynamic weight bearing is important for normal growth and maintenance of the skeleton in humans and laboratory animals. Transforming growth factor-beta (TGF-beta) has been implicated as having autocrine and paracrine actions in bone. The purpose of this study was to examine mRNA levels of TGF-beta in skeletal tissues of growing male rats following skeletal unweighting during an 11-day spaceflight. Animals were sacrificed 5-8 h after the skeleton was reloaded. Spaceflight resulted in decreases in cortical bone area and periosteal bone formation, but no change in medullary area and endocortical bone formation. In addition, spaceflight had no effect on longitudinal bone growth. TGF-beta was reduced relative to the ground controls in the hindlimb periosteum, but was not significantly altered in the growth zone of the tibial metaphysis. Similarly, mRNA levels for type I collagen were reduced in the periosteum, but not in the metaphysis of flight animals. The results suggest a potential role of TGF-beta as an intermediate in the signal transduction pathway for mechanical loading. Further, they indicate skeletal tissue compartment-dependent changes in mRNA levels for TGF-beta following weightlessness.
authors.affiliation: Department of Orthopedics and Biochemistry and Molecular Biology, Mayo Graduate School of Medicine, Rochester, Minnesota, USA.
authors.forename: K C
authors.initials: KC
authors.lastname: Westerlind
authors.forename: R T
authors.initials: RT
authors.lastname: Turner
mesh_terms: D000818:Animals; D001846:Bone Development / Q000502:physiology; D001842:Bone and Bones / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D018483:Diaphyses / Q000502:physiology; D005269:Femur / Q000502:physiology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006614:Hindlimb / Q000502:physiology; D007091:Image Processing, Computer-Assisted; D008297:Male; D010521:Periosteum / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D013026:Space Flight; D013977:Tibia / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000235:genetics*; D016474:Weight-Bearing; D014893:Weightlessness / Q000009:adverse effects*
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases