title: Characterization of the endosymbiont of a deep-sea bivalve, Calyptogena soyoae.
abstract: We have purified DNA from gill tissue of a marine bivalve, Calyptogena soyoae, collected from the deep-sea cold seep communities in Sagami Bay, Japan. An rRNA gene was amplified, cloned, and sequenced. In situ hybridization revealed that the sequence is that of a bacterial endosymbiont within the gill of C. soyoae.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan.
authors.forename: Y W
authors.initials: YW
authors.lastname: Kim
authors.forename: M
authors.initials: M
authors.lastname: Yasuda
authors.forename: A
authors.initials: A
authors.lastname: Yamagishi
authors.forename: T
authors.initials: T
authors.lastname: Oshima
authors.forename: S
authors.initials: S
authors.lastname: Ohta
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D049872:Bivalvia / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005880:Gills / Q000382:microbiology; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013559:Symbiosis
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S