Cloning and sequence of bone morphogenetic protein 4 (BMP-4) from a human placental cDNA library.
A cDNA clone encoding bone morphogenetic protein-4 (BMP-4) has been isolated from a human placental cDNA library. Sequence analysis of this clone revealed that the nucleotide sequence of 5' region was different from that of human osteosarcoma BMP-4 and the deduced amino acid sequence indicated deletion of N-terminal 6 amino acids. We confirmed the expression of this type of BMP-4 mRNA in one human osteogenic cell line in addition to the placenta by the polymerase chain reaction (PCR).
Oida
S
S
Department of Biochemistry, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Iimura
T
T
Maruoka
Y
Y
Takeda
K
K
Sasaki
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019485:Bone Morphogenetic Proteins; D001859:Bone Neoplasms / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D015723:Gene Library; D006133:Growth Substances / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment
D019485:Bone Morphogenetic Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D006133:Growth Substances; D011506:Proteins; D012333:RNA, Messenger