Construction of an integrative shuttle vector for Zymomonas mobilis.
An integrative shuttle vector, pZMOCP1, was constructed by ligating EcoRV digests of the plasmid cloning vector pBluescript and pZMP1, a cryptic plasmid of Zymomonas mobilis PROIMI A1. The 7.2-kb plasmid pZMOCP1 replicated in Escherichia coli and could also be transferred from this host by electroporation to Z. mobilis ATCC 29191. The transformants were selected by ampicillin resistance. The integrative characteristic was detected by hybridization in situ. The vector was stably maintained in Z. mobilis after 200 generations without selective pressure.
Delgado
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OD
PROIMI, Tucumán, Argentina.
Abate
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CM
Siñeriz
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F
D002874:Chromosome Mapping; D018274:Electroporation; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors / Q000737:chemistry / Q000302:isolation & purification*; D010957:Plasmids / Q000737:chemistry / Q000302:isolation & purification*; D015183:Restriction Mapping; D017355:Zymomonas / Q000235:genetics*