A member of the CTF/NF-1 transcription factor family regulates murine growth hormone receptor gene promoter activity.
Deletional analysis by transient transfection of COS-7 cells with the murine growth hormone (GH) receptor gene promoter for the L1 transcript identified a 16-bp enhancer element located approximately 3.0 kb upstream of the major transcription start sites. The sequence of this enhancer element suggested a binding motif for the CTF/NF-1 family of transcription factors. In electromobility shift assays (EMSA) this DNA-element formed a sequence-specific complex with nuclear proteins from COS-7 cells. Competition experiments with oligonucleotide containing a consensus binding motif for CTF/NF-1 protein(s) and supershift EMSA with an anti-CTF/NF-1 antibody established that a CTF/NF-1 like protein binds to this enhancer element.
Zou
L
L
Department of Pediatrics, Children's Hosp. of Pittsburgh, University of Pittsburgh School of Medicine, PA 15213, USA.
Menon
R K
RK
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D001667:Binding, Competitive; D022762:CCAAT-Enhancer-Binding Proteins; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism / Q000494:pharmacology*; D004742:Enhancer Elements, Genetic; D017353:Gene Deletion; D005786:Gene Expression Regulation / Q000187:drug effects*; D051379:Mice; D008969:Molecular Sequence Data; D051839:NFI Transcription Factors; D011401:Promoter Regions, Genetic; D011986:Receptors, Somatotropin / Q000235:genetics*; D014157:Transcription Factors / Q000378:metabolism / Q000494:pharmacology*; D014162:Transfection
D022762:CCAAT-Enhancer-Binding Proteins; C077305:CTF-1 transcription factor; D004268:DNA-Binding Proteins; D051839:NFI Transcription Factors; D011986:Receptors, Somatotropin; D014157:Transcription Factors; D004247:DNA