A model to study cytokine profiles in primary and heterologously secondary Dengue-2 virus infections.
Roles of cytokines in primary and secondary Dengue virus (DV) infections are not completely understood. In this study, we challenged mononuclear leukocytes (MNLs) obtained from non-immune and DV-1-infected donors with DV-2 in vitro to mimic primary and heterologously secondary DV-2 infections, respectively. We found that MNLs in response to DV-2 could release a large amount of interleukin-1 (IL-1 beta) and tumor necrosis factor alpha (TNF alpha). However, IL-1 beta and TNF alpha released by MNLs with primary or heterologously secondary exposure to DV-2 were not significantly different. In contrast, MNLs with heterologously secondary DV-2 infections produced significantly higher amount of interferon gamma (IFN gamma) than those with primary DV-2 infections. These results suggest that IFN gamma, but not TNF alpha or IL-1 beta, may in part participate in the pathogenesis of Dengue shock syndrome (DSS) frequently found in heterologously secondary DV-2 infections.
Yang
K D
KD
Department of Pediatrics, Tri-service General Hospital, Taipei, Republic of China.
Lee
C S
CS
Hwang
K P
KP
Chu
M L
ML
Shaio
M F
MF
D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D003715:Dengue / Q000276:immunology*; D003716:Dengue Virus; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D007375:Interleukin-1 / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D007375:Interleukin-1; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma