Aggregation properties of semisynthetic GD1a ganglioside (IV3Neu5AcII3Neu5AcGgOse4Cer) containing an acetyl group as acyl moiety.
GD1a ganglioside containing an acetyl group as acyl moiety, GD1a(acetyl), was synthesized from natural GD1a. The aggregative properties in aqueous solution of GD1a(acetyl) have been studied by static and dynamic laser light-scattering measurements. GD1a(acetyl) spontaneously aggregates as small micelles showing a hydrodynamic radius and molecular mass of 33 A and 96 kDa, respectively. Vibrio cholerae sialidase showed a very high activity on the micelles of GD1a(acetyl), compared to GD1a. This has been explained as a consequence of the high surface curvature of the the small micelles. High resolution proton NMR spectra were recorded from micelles of GD1a(acetyl) in deuterated water. The low overall correlation time of the GD1a(acetyl) micelles was calculated to be about 2 x 10(-8)s, a value one order of magnitude lower than that determined for natural GD1a.
Brocca
P
P
Department of Medical Chemistry and Biochemistry, Medical School, University of Milan, Italy.
Cantu
L
L
Sonnino
S
S
D015060:1,2-Dipalmitoylphosphatidylcholine; D000107:Acetylation; D005732:Gangliosides / Q000138:chemical synthesis / Q000737:chemistry*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D008970:Molecular Weight; D009439:Neuraminidase / Q000378:metabolism; D010316:Particle Size; D012542:Scattering, Radiation; D013696:Temperature
D005732:Gangliosides; D008823:Micelles; C021298:ganglioside, GD1a; D015060:1,2-Dipalmitoylphosphatidylcholine; D009439:Neuraminidase