Shear stress induces expression of CNP gene in human endothelial cells.
To elucidate the effect of blood flow on gene transcription of C-type natriuretic peptide (CNP), human umbilical vein endothelial cells were exposed to shear stress in a cone-plate viscometer. Expression of CNP mRNA, evaluated by reverse transcription-polymerase chain reaction, was markedly increased by exposure to shear stress of 24 dyne/cm2 at 3 h. The CNP mRNA level was maintained until 12 h. Thus, the present study demonstrated for the first time that shear stress induces expression of CNP gene in human endothelial cells.
Okahara
K
K
Department of Surgery II, Osaka University Medical School, Japan.
Kambayashi
J
J
Ohnishi
T
T
Fujiwara
Y
Y
Kawasaki
T
T
Monden
M
M
D009320:Atrial Natriuretic Factor / Q000096:biosynthesis; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D004730:Endothelium, Vascular / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D020098:Natriuretic Peptide, C-Type; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013314:Stress, Mechanical; D014158:Transcription, Genetic; D014471:Umbilical Veins
D017931:DNA Primers; D012333:RNA, Messenger; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor