Determination of ephedrine compounds in nutritional supplements by cyclodextrin-modified capillary electrophoresis.
Capillary electrophoresis was utilized for the separation, identification, and quantitation of ten stereoisomers in the ephedrine family. Chiral discrimination was accomplished through the use of hydroxypropyl-beta-cyclodextrin, and separation was enhanced at pH 2 in the presence of tetramethylammonium chloride and sodium dodecyl sulfate. Calibration plots of the ephedrines were linear over the range 4-100 micrograms/ml. This method was used in the analysis of nutritional supplements that contain Ma Huang, a Chinese herbal preparation that is made from plants in the genus Ephedra.
Flurer
C L
CL
U.S. Food and Drug Administration, National Forensic Chemistry Center, Cincinnati, OH 45202, USA.
Lin
L A
LA
Satzger
R D
RD
Wolnik
K A
KA
D003505:Cyclodextrins / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004809:Ephedrine / Q000032:analysis*; D005504:Food Analysis; D013237:Stereoisomerism
D003505:Cyclodextrins; D004809:Ephedrine