The first isolation of Borrelia burgdorferi from Ixodes ricinus (Acari, Ixodidae) ticks in Poland.
Among ticks Ixodes ricinus collected in April 1994 in the forest near Fraknowo (Olsztyn province, N. C. Poland), 17 ticks were evaluated for Borrelia infection by cultivating in the BSK-H medium (Sigma). The ticks were examined in five pools--four of 3 females and one pool of 5 nymphs. Spirochaetes were cultured successfully only from one pool of females and identified as Borrelia burgdorferi with monoclonal antibodies: H 9724, H 5332, H 3TS and 11 G 1 by indirect immunofluorescence assay (IFA). This is the first reported isolate of B. burgdorferi from Ixodes ricinus in Poland.
Dabrowski
J
J
Institute of Maritime and Tropical Medicine, Gdynia, Poland.
Schönberg
A
A
Wegner
Z
Z
Stańczak
J
J
Kruminis-Lozowska
W
W
D000818:Animals; D000911:Antibodies, Monoclonal; D001096:Arachnid Vectors / Q000382:microbiology*; D015748:Borrelia burgdorferi Group / Q000302:isolation & purification*; D004197:Disease Reservoirs; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D018884:Ixodes / Q000382:microbiology*; D051379:Mice; D009758:Nymph; D011044:Poland; D011817:Rabbits
D000911:Antibodies, Monoclonal