Analysis of pyrrolizidine alkaloids: a competitive enzyme-linked immunoassay (ELISA) for the quantitative determination of some toxic pyrrolizidine alkaloids.
A highly sensitive and specific competitive enzyme-linked immunoassay (ELISA) for the determination of retrorsine, senecionine, and integerrimine in the nanogram range has been developed. Pyrrolizidine alkaloids were detected in Senecio rupestris. No cross-reactivity was seen with either retrosine N-oxide, otonecine alkaloids, or other macrocyclic pyrrolizidine alkaloids.
Roeder
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Pharmazeutisches Institut, Universität Bonn, Germany.
Pflueger
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T
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D011763:Pyrrolizidine Alkaloids / Q000032:analysis*; D011817:Rabbits; D012015:Reference Standards
D011763:Pyrrolizidine Alkaloids; C003300:retrorsine