title: Rat skeletal muscle selenoprotein W: cDNA clone and mRNA modulation by dietary selenium.
abstract: Rat skeletal muscle selenoprotein W cDNA was isolated and sequenced. The isolation strategy involved design of degenerate PCR primers from reverse translation of a partial peptide sequence. A reverse transcription-coupled PCR product from rat muscle mRNA was used to screen a muscle cDNA library prepared from selenium-supplemented rats. The cDNA sequence confirmed the known protein primary sequence, including a selenocysteine residue encoded by TGA, and identified residues needed to complete the protein sequence. RNA folding algorithms predict a stem-loop structure in the 3' untranslated region of the selenoprotein W mRNA that resembles selenocysteine insertion sequence (SE-CIS) elements identified in other selenocysteine coding cDNAs. Dietary regulation of selenoprotein W mRNA was examined in rat muscle. Dietary selenium at 0.1 ppm as selenite increased muscle mRNA 4-fold relative to a selenium-deficient diet. Higher dietary selenium produced no further increase in mRNA levels.
authors.affiliation: Department of Agricultural Chemistry, Oregon State University, Corvallis 97331, USA.
authors.forename: S C
authors.initials: SC
authors.lastname: Vendeland
authors.forename: M A
authors.initials: MA
authors.lastname: Beilstein
authors.forename: J Y
authors.initials: JY
authors.lastname: Yeh
authors.forename: W
authors.initials: W
authors.lastname: Ream
authors.forename: P D
authors.initials: PD
authors.lastname: Whanger
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000235:genetics; D004032:Diet; D004305:Dose-Response Relationship, Drug; D005260:Female; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000096:biosynthesis / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000378:metabolism* / Q000494:pharmacology; D017279:Selenocysteine / Q000235:genetics; D051151:Selenoprotein W; D051140:Selenoproteins
chemical_list: D018076:DNA, Complementary; D009124:Muscle Proteins; D011506:Proteins; D012333:RNA, Messenger; D051151:Selenoprotein W; D051140:Selenoproteins; D017279:Selenocysteine; D012643:Selenium