Exercise-induced changes in the expression of surface adhesion molecules on circulating granulocytes and lymphocytes subpopulations.
This study examined the relationship between exercise-induced changes in the concentration of circulating immunocompetent cells and their surface expression of adhesion molecules: L-selectin (CD62L) and three beta 2-integrins [LFA-1(CD11a/CD18), Mac-1 (CD11b/CD18), and p150/95(CD11c/CD18)]. Eight young male volunteers exercised on a cycle ergometer for 60 min at 60% maximal oxygen uptake. Peripheral blood samples, collected every 30 min throughout exercise and during the 2-h recovery period, were used for flow-cytometric analysis. The experimental results were compared with control data obtained ever 60 min at corresponding times of the nonexercise day. The exercise regimen induced a granulocytosis and a lymphocytosis, mainly due to an elevation of CD8+ and CD16+ cells. During recovery, a further granulocytosis occurred but accompanied by a lymphopenia. The increased CD8+ cell-count during exercise was characterized by a selective mobilization of the CD62L- and CD11ahigh cells, i.e. primed CD8+ cells. A postexercise suppression of CD4+ cell-count was derived only from CD62L+ cells. The CD11b+ and CD11c+ lymphocytes also increased during exercise, largely attributable to an increase in CD16+ cells which co-expressed CD11b and CD11c molecules. The CD62L surface density of granulocytes increased significantly during recovery. This resulted from a selective influx of CD62Lhigh granulocytes into the circulation. There were no significant changes in per-cell density of the three beta 2-integrins on granulocytes and lymphocytes throughout the experimental period. These results suggest that the cell-surface expression of CD62L (and CD11a) molecules is associated with the differential mobilization of CD8+ cells during exercise, the postexercise suppression of CD4+ cell-counts and the granulocytosis following exercise.
Kurokawa
Y
Y
Department of Hygiene and Public Health, Ehime University School of Medicine, Japan.
Shinkai
S
S
Torii
J
J
Hino
S
S
Shek
P N
PN
D000328:Adult; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D016167:Integrin alphaXbeta2 / Q000378:metabolism; D019041:L-Selectin / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology; D018655:Lymphocyte Count; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D016131:Lymphocyte Subsets / Q000166:cytology; D008214:Lymphocytes / Q000378:metabolism*; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008297:Male; D005082:Physical Exertion
D015815:Cell Adhesion Molecules; D016167:Integrin alphaXbeta2; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D019041:L-Selectin