title: Characterization of human buccal epithelial cells transfected with the simian virus 40 T-antigen gene.
abstract: Serum-free cultures of normal human buccal epithelial cells were transfected with a plasmid containing the SV40 T-antigen (SV40T) gene. Two major lines developed that showed extended lifespans (between 30 and 40 weeks) as compared with the controls (approximately 6 weeks). Continued growth through one or two crises generated several sublines. They expressed the epithelial marker keratin and also exhibited nuclear expression of SV40T. The lines showed abnormal karyotypes with both numerical and structural aberrations and variably responded to agents that normally inhibit growth and/or induce terminal differentiation, i.e. transforming growth factor-beta 1 and fetal bovine serum. One of the lines, termed SVpgC2a, developed into an apparently immortal line, since it had undergone more than 700 population doublings from over 2 years in culture. Further characterization of this line demonstrated its clonal origin, with integration of two copies of SV40T at the same site and the presence of both normal retinoblastoma and wild-type p53 proteins. This line showed high resistance to growth inhibition by transforming growth factor-beta 1 and serum similar to that shown by buccal carcinoma cell line SqCC/Y1. Neither SVpgC2a nor its parental lines were tumorigenic when injected into athymic nude mice, whereas the SqCC/Y1 cells induced tumors. The various lines with extended but finite lifespans, complemented by one immortalized line, which retained non-malignant properties upon extended culture, provide a battery of model systems that will be useful for studying mechanisms of human oral carcinogenesis.
authors.affiliation: Institute of Environmental Medicine, Karolinska Institute, Stockholm, Sweden.
authors.forename: P S
authors.initials: PS
authors.lastname: Kulkarni
authors.forename: K
authors.initials: K
authors.lastname: Sundqvist
authors.forename: C
authors.initials: C
authors.lastname: Betsholtz
authors.forename: P
authors.initials: P
authors.lastname: Höglund
authors.forename: K G
authors.initials: KG
authors.lastname: Wiman
authors.forename: B
authors.initials: B
authors.lastname: Zhivotovsky
authors.forename: F
authors.initials: F
authors.lastname: Bertolero
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: R C
authors.initials: RC
authors.lastname: Grafström
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000096:biosynthesis / Q000235:genetics*; D015415:Biomarkers / Q000032:analysis; D001769:Blood; D002417:Cattle; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism; D005814:Genes, Viral; D006801:Humans; D007621:Karyotyping; D007633:Keratins / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008819:Mice, Nude; D009061:Mouth Mucosa / Q000166:cytology / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000473:pathology / Q000503:physiopathology; D011994:Recombinant Proteins / Q000096:biosynthesis; D016160:Retinoblastoma Protein / Q000096:biosynthesis; D013539:Simian virus 40 / Q000235:genetics*; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis; D016662:Virus Integration
chemical_list: D000952:Antigens, Polyomavirus Transforming; D015415:Biomarkers; D016895:Culture Media, Serum-Free; D011994:Recombinant Proteins; D016160:Retinoblastoma Protein; D016212:Transforming Growth Factor beta; D016159:Tumor Suppressor Protein p53; D007633:Keratins