Plasticity of myofibroblasts appearing in granulation tissues after acetic acid treatment. Effect of bFGF.
To clarify the origin of the myofibroblasts appearing in the healing process of the acetic acid-induced ulcer and effect of basic fibroblast growth factor (bFGF) on these myofibroblasts, we conducted an immunohistochemical study using antibody to intermediate filaments, desmin and vimentin. The binding sites of bFGF on the regenerative tissues were also studied by the radioautographic study of soluble compounds. As a result, the binding sites of bFGF were accumulated on the fibroblasts and myofibroblasts as well as on endothelial cells. The effect of CS23, acid-stable human recombinant bFGF was shown on distribution of myofibroblasts and regeneration of the microvascular system in the mucosal and submucosal layers.
Nakamura
M
M
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
Oda
M
M
Inoue
J
J
Ito
T
T
Akiba
Y
Y
Kitajima
M
M
Tsuchiya
M
M
Ishii
H
H
D000085:Acetates; D019342:Acetic Acid; D000818:Animals; D001345:Autoradiography; D003893:Desmin / Q000032:analysis; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism / Q000494:pharmacology*; D005347:Fibroblasts / Q000737:chemistry / Q000473:pathology*; D005753:Gastric Mucosa / Q000098:blood supply; D006097:Granulation Tissue / Q000737:chemistry / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D008833:Microcirculation; D009130:Muscle, Smooth / Q000737:chemistry / Q000473:pathology; D051381:Rats; D011994:Recombinant Proteins; D013276:Stomach Ulcer / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D014746:Vimentin / Q000032:analysis
D000085:Acetates; D003893:Desmin; D011994:Recombinant Proteins; D014746:Vimentin; D016222:Fibroblast Growth Factor 2; D019342:Acetic Acid