Age-dependent changes in voltage-gated calcium channels and ATP-dependent potassium channels in Fischer 344 rats.
1. Radioligand binding and 45Ca2+ uptake measurements quantitated ion channel numbers and properties in brain membranes from Fischer 344 rats at 6, 12, 18 and 30 months of age. 2. Decreases in 1,4-dihydropyridine density occurred in striatum, hippocampus and cortex with a decreased affinity. 3. Decreases in w-conotoxin binding occurred in hippocampus and striatum with an increase in affinity. 4. K+ depolarization-mediated 45Ca2+ uptake decreased only in striatum at 18 months. 5. Decreases in glibenclamide binding occurred in cortex and cerebellum at 12-30 months. 6. No changes in 1,4-dihydropyridine binding occurred with age in heart, but glibenclamide binding density was significantly decreased at 30 months.
Bangalore
R
R
School of Pharmacy, State University of New York, Buffalo 14260, USA.
Triggle
D J
DJ
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000375:Aging; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology*; D005905:Glyburide / Q000494:pharmacology; D006339:Heart Rate / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects; D017275:Isradipine / Q000494:pharmacology; D008297:Male; D015221:Potassium Channels / Q000187:drug effects / Q000502:physiology*; D011869:Radioligand Assay; D051381:Rats; D011916:Rats, Inbred F344
D015220:Calcium Channels; D015221:Potassium Channels; D000255:Adenosine Triphosphate; D005905:Glyburide; D002118:Calcium; D017275:Isradipine