title: Experimental study of the biocompatibility of four root canal sealers and their influence on the zinc and calcium content of several tissues.
abstract: Four root canal sealers (AH-26, Roth 811, CRCS, and Sealapex) were tested for tissue biocompatibility in rat connective tissue. Each sealer was placed in Teflon tubes and implanted subcutaneously in Wistar-Furth rats. The implants were removed after 7, 14, and 21 days, fixed, and histologically prepared for microscopical evaluation. Brain, liver, kidneys, and uterus were removed from the animals killed at the first experimental period (7 days) and analyzed for zinc and calcium concentration by flame atomic absorption spectrophotometry. In total, 100 specimens were examined. At the seventh day, the most irritant material was seen to be AH-26, but this inflammatory reaction decreased with time. Roth 811 and Sealapex caused moderate-to-severe inflammatory reaction, whereas CRCS caused mild to moderate. CRCS and Roth 811 induced redistribution of zinc, whereas AH-26 induced changes in calcium content in some organs.
authors.affiliation: Department of Dental Pathology and Therapeutics, Faculty of Dentistry, Aristotelian University of Thessaloniki, Greece.
authors.forename: N
authors.initials: N
authors.lastname: Economides
authors.forename: V P
authors.initials: VP
authors.lastname: Kotsaki-Kovatsi
authors.forename: A
authors.initials: A
authors.lastname: Poulopoulos
authors.forename: I
authors.initials: I
authors.lastname: Kolokuris
authors.forename: G
authors.initials: G
authors.lastname: Rozos
authors.forename: R
authors.initials: R
authors.lastname: Shore
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000493:pharmacokinetics; D001729:Bismuth / Q000009:adverse effects / Q000493:pharmacokinetics; D001923:Brain Chemistry; D002118:Calcium / Q000032:analysis; D002126:Calcium Hydroxide / Q000009:adverse effects / Q000493:pharmacokinetics; D003238:Connective Tissue / Q000187:drug effects* / Q000378:metabolism; D004338:Drug Combinations; D004853:Epoxy Resins; D005260:Female; D005549:Foreign-Body Reaction / Q000209:etiology; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008709:Methenamine / Q000009:adverse effects / Q000493:pharmacokinetics; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000009:adverse effects* / Q000493:pharmacokinetics; D012459:Salicylates; D012834:Silver / Q000009:adverse effects / Q000493:pharmacokinetics; D014018:Tissue Distribution; D014025:Titanium / Q000009:adverse effects / Q000493:pharmacokinetics; D014599:Uterus / Q000737:chemistry; D015032:Zinc / Q000032:analysis; D015034:Zinc Oxide / Q000009:adverse effects / Q000493:pharmacokinetics; D015035:Zinc Oxide-Eugenol Cement / Q000009:adverse effects / Q000493:pharmacokinetics
chemical_list: D001672:Biocompatible Materials; D004338:Drug Combinations; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; D012459:Salicylates; D015035:Zinc Oxide-Eugenol Cement; C055601:Sealapex; C075563:Calcibiotic Root Canal Sealer; D012834:Silver; C028044:epoxy resin AH-26; C095719:Roth's 811 sealer; D014025:Titanium; D015032:Zinc; D008709:Methenamine; D002126:Calcium Hydroxide; D015034:Zinc Oxide; D002118:Calcium; D001729:Bismuth