A ribosome-associated peptidyl-prolyl cis/trans isomerase identified as the trigger factor.
Peptidyl-prolyl cis/trans isomerases (PPIases) are enzymes that catalyse protein folding both in vitro and in vivo. We isolated a peptidyl-prolyl cis/trans isomerase (PPIase) which is specifically associated with the 50S subunit of the Escherichia coli ribosome. This association was abolished by adding at least 1.5 M LiCl. Sequencing the N-terminal amino acids in addition to three proteolytic fragments totalling 62 amino acids revealed that this PPIase is identical to the E.coli trigger factor. A comparison of the amino acid sequence of trigger factor with those of other PPIase families shows little similarities, suggesting that trigger factor may represent an additional family of PPIases. Trigger factor was purified to homogeneity on a preparative scale from E.coli and its enzymatic properties were studied. In its activity towards oligopeptide substrates, the trigger factor resembles the FK506-binding proteins (FKBPs). Additionally, the pattern of subsite specificities with respect to the amino acid preceding proline in Suc-Ala-Xaa-Pro-Phe-4-nitroanilides is reminiscent of FKBPs. However, the PPIase activity of the trigger factor was not inhibited by either FK506 or by cyclosporin A at concentrations up to 100 microM. In vitro, the trigger factor catalysed the proline-limited refolding of a variant of RNase T1 much better than all other PPIases that have been examined so far.
Stoller
G
G
Max-Planck-Gesellschaft, Arbeitsgruppe Enzymologie der Peptidbindung, Berlin, Germany.
Rücknagel
K P
KP
Nierhaus
K H
KH
Schmid
F X
FX
Fischer
G
G
Rahfeld
J U
JU
D000591:Amino Acid Isomerases / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D002451:Cell Compartmentation; D004268:DNA-Binding Proteins; D004926:Escherichia coli / Q000201:enzymology*; D006360:Heat-Shock Proteins; D007536:Isomerism; D018021:Lithium Chloride / Q000494:pharmacology; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000378:metabolism; D019696:Peptidylprolyl Isomerase; D017510:Protein Folding; D006163:Ribonuclease T1 / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000201:enzymology*; D017421:Sequence Analysis; D012995:Solubility; D013379:Substrate Specificity; D016559:Tacrolimus / Q000494:pharmacology; D022021:Tacrolimus Binding Proteins
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D009842:Oligopeptides; D006163:Ribonuclease T1; D000591:Amino Acid Isomerases; D022021:Tacrolimus Binding Proteins; D019696:Peptidylprolyl Isomerase; D018021:Lithium Chloride; D016559:Tacrolimus