Influence of human luteinizing hormone on cell growth and CA 125 secretion of primary epithelial ovarian carcinomas in vitro.
In the present study, the influence of human luteinizing hormone(hLH) on the growth and the CA 125 secretion of primary ovarian carcinoma cell cultures derived from 11 previously untreated patients was investigated. Two different patterns of in vitro growth of unstimulated ovarian carcinoma cells could be detected: 5 cell cultures in which no changes of cell number were observed during an incubation period of 6 days (group A) and 6 cell cultures which grew without any hormonal support (group B). All tumors of group A showed enhanced cell proliferation when hLH was added to the culture medium, reaching a maximum at dosages of 100 mIU/ml on day 2 after incubation, while such an effect was not observed in group B. In most cases, no positive correlation was found between hLH-induced cell growth and CA 125 release. Our findings demonstrated a dose-dependent hLH-induced stimulation of epithelial ovarian tumors in vitro.
Kurbacher
C M
CM
Department of Obstetrics and Gynecology, University of Erlangen-Nürnberg, Germany.
Jäger
W
W
Kurbacher
J A
JA
Bittl
A
A
Wildt
L
L
Lang
N
N
D000230:Adenocarcinoma / Q000473:pathology / Q000503:physiopathology; D000368:Aged; D000369:Aged, 80 and over; D018394:CA-125 Antigen / Q000096:biosynthesis*; D002277:Carcinoma / Q000473:pathology* / Q000503:physiopathology; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival; D003470:Culture Media; D005260:Female; D006801:Humans; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008875:Middle Aged; D015999:Multivariate Analysis; D010051:Ovarian Neoplasms / Q000473:pathology* / Q000503:physiopathology*; D014407:Tumor Cells, Cultured
D018394:CA-125 Antigen; D003470:Culture Media; D007986:Luteinizing Hormone