Induction of HSP72 in rat liver by chronic ethanol consumption combined with exercise: association with the prevention of ethanol-induced fatty liver by exercise.
Ethanol-induced fatty liver in rats was attenuated by repeated running exercise, and the protective effect of exercise was associated with the synergistic expression of heat shock proteins (HSP72). Rats were placed in four groups of six. The two ethanol-fed groups of rats received a liquid diet (Lieber-DeCarli formulation) in which 36% of the calories were derived from ethanol. One group remained sedentary (S/E), whereas the other was trained to run on a rodent treadmill at a speed of 27 m/min, 1 hr/day, 5 days/week, for 7 weeks (R/E). Two other groups--one exercised as previously mentioned (R/C) and one sedentary (S/C)--received control-liquid diets in which the ethanol was isocalorically substituted with a dextran/maltose mixture. The degree of fatty infiltration in liver sections stained with hematoxylin and eosin was graded on a 0-4 scale and the data analyzed by ANOVA on ranks. Ethanol significantly induced fatty infiltration in the S/E group, whereas fatty infiltration in the livers of the R/E group was not different from the S/C group. Electrophoresis and Western blotting of liver homogenates demonstrated that HSP72 was not expressed in either the S/C or S/E groups and was only slightly expressed in the R/C group. The combination of exercise and ethanol, however, resulted in an elevated expression of HSP72 in the R/E group. The content of HSP73 was unaffected by any treatment.
Trudell
J R
JR
Department of Anesthesia, Stanford University School of Medicine, California, USA.
Lin
W Q
WQ
Chrystof
D A
DA
Kirshenbaum
G
G
Ardies
C M
CM
D000818:Animals; D000431:Ethanol / Q000633:toxicity; D005235:Fatty Liver, Alcoholic / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology*; D008297:Male; D005082:Physical Exertion / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D000431:Ethanol