title: Dehalogenation of haloalkanes by Rhodococcus erythropolis Y2. The presence of an oxygenase-type dehalogenase activity complements that of an halidohydrolase activity.
abstract: Rhodococcus erythropolis Y2 produced two types of dehalogenase: a hydrolytic enzyme, that is an halidohydrolase, which was induced by C3 to C6 1-haloalkane substrates, and at least one oxygenase-type dehalogenase induced by C7 to C16 1-haloalkanes and n-alkanes. The oxygenase-type activity dehalogenated C4 to C18 1-chloroalkanes with an optimum activity towards 1-chlorotetradecane. The halidohydrolase catalysed the dehalogenation of a wide range of 1- and alpha,omega-disubstituted haloalkanes and alpha,omega-substituted haloalcohols. In resting cell suspensions of hexadecane-grown R. erythropolis Y2 the oxygenase-type dehalogenase had a specific activity of 12.9 mU (mg protein)-1 towards 1-chlorotetradecane (3.67 mU mg-1 towards 1-chlorobutane) whereas the halidohydrolase in 1-chlorobutane-grown batch cultures had a specific activity of 44 mU (mg protein)-1 towards 1-chlorobutane. The significance of the two dehalogenase systems in a single bacterial strain is discussed in terms of their contribution to the overall catabolic potential of the organism.
authors.affiliation: Research School of Biosciences, University of Kent at Canterbury, UK.
authors.forename: S J
authors.initials: SJ
authors.lastname: Armfield
authors.forename: P J
authors.initials: PJ
authors.lastname: Sallis
authors.forename: P B
authors.initials: PB
authors.lastname: Baker
authors.forename: A T
authors.initials: AT
authors.lastname: Bull
authors.forename: D J
authors.initials: DJ
authors.lastname: Hardman
mesh_terms: D000473:Alkanes / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D004790:Enzyme Induction; D006219:Halogens / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000145:classification / Q000378:metabolism*; D008854:Microscopy, Electron; D011422:Propionates / Q000378:metabolism*; D012240:Rhodococcus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure; D013379:Substrate Specificity
chemical_list: D000473:Alkanes; D006219:Halogens; D011422:Propionates; D006867:Hydrolases; C007022:3-chloropropionic acid