Use of haloacetate dehalogenase genes as selection markers for Escherichia coli and Pseudomonas vectors.
The haloacetate dehalogenase gene, dehH2, cloned from Moraxella sp. strain B could be used a selection marker gene for vectors in Escherichia coli and Pseudomonas putida. Haloacetates, especially iodoacetate, inhibit the growth of some microorganisms. The dehH2 gene introduced into the cells conferred iodoacetate resistance on them. Therefore, E. coli and P. putida transformed with vectors marked with dehH2 could be easily selected on plates containing iodoacetate.
Kawasaki
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Department of Agricultural Chemistry, University of Osaka Prefecture, Japan.
Kuriyama
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Tonomura
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D000085:Acetates / Q000494:pharmacology; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D005819:Genetic Markers; D005822:Genetic Vectors; D006867:Hydrolases / Q000235:genetics*; D007461:Iodoacetates / Q000494:pharmacology*; D019807:Iodoacetic Acid; D011549:Pseudomonas / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D012641:Selection, Genetic
D000085:Acetates; D005819:Genetic Markers; D007461:Iodoacetates; D006867:Hydrolases; C023098:haloacetate dehalogenase; D019807:Iodoacetic Acid