Blood monocytes in rheumatoid arthritis are highly adherent to cultured endothelium.
Monocytes from 17 patients with rheumatoid arthritis (RA) were more adherent than monocytes from 17 control patients to monolayers of pig aortic endothelium irrespective of whether sera was included (median 27-34% increase; p = 0.002) or omitted (median 27% increase; p = 0.022) from the culture media. When human umbilical vein endothelial cells were used as the adherence substrate, rheumatoid monocytes from an additional 21 patients demonstrated a median 31% (p = 0.004) and 20% increase (p = 0.004) in adhesion when compared with monocytes from 21 normal healthy subjects in the absence and presence of autologous sera, respectively. Activation of control monocytes with muramyl dipeptide or treatment with RA sera increased their attachment to endothelium (mean 34 +/- 14% increase; p < 0.001). The expression of the adhesion molecules CD11b (p < 0.005), CD18 (p < 0.005), CD62L (p = 0.01) was enhanced on rheumatoid monocytes, but antibody-blocking studies suggested that CD18 and CD62L were not responsible for the augmented binding of the rheumatoid cells. A subpopulation of rheumatoid monocytes possessed a very low net negative surface charge, a property that favours binding to vessel walls. We propose that many rheumatoid monocytes are predisposed for sheer-resistant adhesion to vascular endothelium.
Mazure
G
G
Department of Immunology, UMDS, London, UK.
Fernandes
T
T
McCarthy
D A
DA
Macey
M
M
Perry
J D
JD
Taub
N A
NA
Dumonde
D C
DC
Brown
K A
KA
D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000276:immunology; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000276:immunology / Q000473:pathology*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000276:immunology; D002478:Cells, Cultured; D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000276:immunology / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology / Q000473:pathology*; D013552:Swine
D015703:Antigens, CD; D015815:Cell Adhesion Molecules