Bacterial degradation of m-nitrobenzoic acid.
Pseudomonas sp. strain JS51 grows on m-nitrobenzoate (m-NBA) with stoichiometric release of nitrite. m-NBA-grown cells oxidized m-NBA and protocatechuate but not 3-hydroxybenzoate, 4-hydroxy-3-nitrobenzoate, 4-nitrocatechol, and 1,2,4-benzenetriol. Protocatechuate accumulated transiently when succinate-grown cells were transferred to media containing m-NBA. Respirometric experiments indicated that the conversion of m-NBA to protocatechuate required 1 mol of oxygen per mol of substrate. Conversions conducted in the presence of 18O2 showed the incorporation of both atoms of molecular oxygen into protocatechuate. Extracts of m-NBA-grown cells cleaved protocatechuate to 2-hydroxy-4-carboxymuconic semialdehyde. These results provide rigorous proof that m-NBA is initially oxidized by a dioxygenase to produce protocatechuate which is further degraded by a 4,5-dioxygenase.
Nadeau
L J
LJ
Armstrong Laboratory, Tyndall Air Force Base, Florida 32403-5323, USA.
Spain
J C
JC
D001673:Biodegradation, Environmental; D003470:Culture Media; D062385:Hydroxybenzoates / Q000378:metabolism; D008956:Models, Chemical; D009579:Nitrobenzoates / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010103:Oxygen Isotopes; D011549:Pseudomonas / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D014865:Waste Disposal, Fluid
D003470:Culture Media; D062385:Hydroxybenzoates; D009579:Nitrobenzoates; D010103:Oxygen Isotopes; C009091:protocatechuic acid; C040770:3-nitrobenzoic acid