title: Structural features of archaebacterial and eukaryotic proteasomes.
abstract: The 26S proteasome is the central protease of the ubiquitin-dependent pathway of protein degradation. The molecule has a molecular mass of approximately 2000 kD and has a highly conserved structure in eukaryotes. The 26S proteasome is formed by a barrel-shaped 20S core complex and two polar 19S complexes. The 20S complex has C2 symmetry and is formed by four seven-membered rings of which the outer rings (alpha-type subunits) are rotated by 25.7 degrees relative to the inner rings while the inner rings (beta-type subunits) are in register. From a comparison of the activity and regulation of the 26S and 20S particles it can be deduced that the 20S particle contains the protease activity while the 19S complex contains isopeptidase, ATPase and protein unfolding activities. In this article we describe the structures of various proteasome complexes as determined by electron microscopy and discuss structural implications of their subunit sequences.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried, Germany.
authors.forename: A J
authors.initials: AJ
authors.lastname: Koster
authors.forename: J
authors.initials: J
authors.lastname: Walz
authors.forename: A
authors.initials: A
authors.lastname: Lupas
authors.forename: W
authors.initials: W
authors.lastname: Baumeister
mesh_terms: D000818:Animals; D019843:Archaeal Proteins; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000648:ultrastructure; D010450:Endopeptidases / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D009097:Multienzyme Complexes / Q000737:chemistry / Q000648:ultrastructure; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000648:ultrastructure; D046988:Proteasome Endopeptidase Complex; D011487:Protein Conformation; D013822:Thermoplasma / Q000201:enzymology
chemical_list: D019843:Archaeal Proteins; D009097:Multienzyme Complexes; D010450:Endopeptidases; D010447:Peptide Hydrolases; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease; C093138:proteasome, Thermoplasma