title: Structural analysis of invasion plasmid antigen D (IpaD) from Shigella flexneri.
abstract: Invaison plasmid antigen D (IpaD) and water-extracted outer-membrane proteins (OMPs) from Shigella flexneri were used to investigate some of the structural relationships of this pathogen's invasions. Extracellular presentation of the three invasion plasmid antigens (Ipa) B, C, and D is required for the S. flexneri invasive phenotype; however, little is known of the structural properties of these essential virulence components. Biochemical data suggest IpaB, C, and D present in S. flexneri OMPs from soluble protein complexes and IpaD may be able to form large homopolymeric complexes.
authors.affiliation: Department of Biology, Saint Louis University, MO 63103-2010, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Marquart
authors.forename: W L
authors.initials: WL
authors.lastname: Picking
authors.forename: W D
authors.initials: WD
authors.lastname: Picking
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry*; D003001:Cloning, Molecular; D004735:Energy Transfer; D004926:Escherichia coli; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D005798:Genes, Bacterial; D010641:Phenotype; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D012763:Shigella flexneri / Q000235:genetics / Q000472:pathogenicity*; D013050:Spectrometry, Fluorescence; D014774:Virulence / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D005456:Fluorescent Dyes; C095770:IpaD protein, Shigella flexneri; D016650:Fluorescein-5-isothiocyanate