Polyethylene glycol-modified avidin: a novel agent for the selective extraction of biotinylated immune-complex in an aqueous two-phase system.
Chicken avidin was chemically modified with 2,4-bis[O-methoxypoly(ethylene glycol)]-6-chloro-s-triazine (activated PEG2) to form PEG-avidin. The PEG-avidin, in which 78% of the amino groups were modified, retained 49% of the active biotin-binding sites. The modified avidin was partitioned preferentially into the PEG-phase in an aqueous two-phase system (PEG/dextran). Using PEG-avidin, the immune-complex formed between biotinylated anti-mouse IgG and its antigen IgG (mouse) molecules, was successfully transferred into the PEG-phase in an aqueous two-phase system. This finding leads to the effective isolation of a specific antigen among various kinds of antigens by partitioning with a two-phase system using PEG-avidin.
Nishimura
H
H
Department of Material Science and Technology, Toin University of Yokohama, Japan.
Munakata
N
N
Hayashi
K
K
Hayakawa
M
M
Iwamoto
H
H
Terayama
S
S
Takahata
Y
Y
Kodera
Y
Y
Tsurui
H
H
Shirai
T
T
D000818:Animals; D000936:Antigen-Antibody Complex / Q000276:immunology / Q000302:isolation & purification*; D000941:Antigens / Q000302:isolation & purification; D001360:Avidin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D001665:Binding Sites; D001710:Biotin / Q000737:chemistry; D002645:Chickens; D003911:Dextrans / Q000737:chemistry; D016503:Drug Delivery Systems; D004529:Egg White; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
D000936:Antigen-Antibody Complex; D000941:Antigens; D003911:Dextrans; D007074:Immunoglobulin G; D001360:Avidin; D011092:Polyethylene Glycols; D001710:Biotin