title: Acute and chronic effects of transient myocardial ischemia on sympathetic nerve activity, density, and norepinephrine content.
abstract: OBJECTIVE
  The sympathetic nervous system has profound influences on myocardial function, particularly during ischemia. There is controversy, however, as to whether myocardial ischemia results in damage to myocardial sympathetic nerves coursing through the ischemic territory. To further evaluate these issues, we assessed the acute and chronic effects of transient myocardial ischemia on sympathetic nerve function and morphology.
  
  
  METHODS
  A total of 20 dogs were studied. For acute studies (n = 9), we performed serial dynamic imaging of I-123 metaiodobenzylguanidine (MIBG) washout during coronary occlusion and reperfusion, and assessed residual myocardial perfusion with thallium-201. For chronic studies (n = 11), we assessed sympathetic innervation and perfusion 11 days following a transient intracoronary balloon occlusion. Imaging results were correlated with electrocardiographic responses, histology, and tissue norepinephrine (NE).
  
  
  RESULTS
  In the acute studies, regional MIBG washout increased more than 2-fold in the ischemic territory compared to the control region during coronary occlusion (14.2 +/- 2.3 vs. 5.9 +/- 1.2%, P < 0.01). Tissue NE was reduced in the ischemic territory compared to the non-ischemic territory (335 +/- 162 vs. 751 +/- 190 ng/g, P < 0.01). Myocardial perfusion was normal. In the chronic studies, 9/11 dogs showed ischemic ECG changes during balloon occlusion, and developed ventricular arrhythmias. On follow-up imaging, 5/11 dogs showed reduced MIBG uptake relative to thallium, in viable myocardium overlying necrotic subendocardium, reduced NE (226 +/- 77 vs. 733 +/- 82 ng/g in control regions, P < 0.01), decreased nerve density, and a larger extent of denervation than scar (25.5 +/- 3.7 vs. 8.2 +/- 2.7%, P < 0.02). Six of 11 dogs showed normal innervation patterns.
  
  
  CONCLUSIONS
  These studies suggest that the sympathetic nerves are acutely affected in regions of myocardial ischemia as detected by enhanced regional washout of MIBG. In addition, chronic sympathetic nerve denervation can occur in the absence of transmural myocardial necrosis; however, the occurrence of transient ischemia does not predict the development of chronic denervation. The severity of ischemia, as evidenced by the extent of the related necrosis, does appear to predict chronic denervation. The severity of ischemia, as evidenced by the extent of the related necrosis, does appear to predict chronic denervation. The mechanisms leading to chronic denervation of sympathetic nerves in the absence of transmural infarction remain to be defined.
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco 94143, USA.
authors.forename: M W
authors.initials: MW
authors.lastname: Dae
authors.forename: J W
authors.initials: JW
authors.lastname: O'Connell
authors.forename: E H
authors.initials: EH
authors.lastname: Botvinick
authors.forename: M C
authors.initials: MC
authors.lastname: Chin
mesh_terms: D019797:3-Iodobenzylguanidine; D000208:Acute Disease; D000818:Animals; D002908:Chronic Disease; D004285:Dogs; D004562:Electrocardiography; D006321:Heart / Q000000981:diagnostic imaging; D007091:Image Processing, Computer-Assisted; D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D008856:Microscopy, Fluorescence; D017202:Myocardial Ischemia / Q000000981:diagnostic imaging / Q000378:metabolism / Q000503:physiopathology*; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism*; D011877:Radionuclide Imaging; D013564:Sympathetic Nervous System / Q000503:physiopathology*; D013566:Sympathomimetics / Q000378:metabolism*
chemical_list: D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D013566:Sympathomimetics; D019797:3-Iodobenzylguanidine; D009638:Norepinephrine