Brief overview of control of genetic expression by antisense oligonucleotides and in vivo applications. Prospects for neurobiology.
Over the past several years, the use of synthetic oligonucleotides and functional analogs thereof as a possibly general means of controlling genetic expression has received widespread attention. Following a brief overview of some of the basic principles and strategies for this approach, attention is focused here on summarizing some recent reports of in vitro and, in particular, in vivo investigations in various animal models using phosphorothioate analogs of 2'-deoxyoligonucleotides. In view of these findings, which include studies related to neurobiology, this field should find significant utility in applications of the antisense method for controlling genetic expression.
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Lynx Therapeutics, Inc., Hayward, CA 94545, USA.
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002986:Clinical Trials as Topic; D005544:Forecasting; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009369:Neoplasms / Q000628:therapy; D009374:Neoplasms, Experimental / Q000628:therapy; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D012313:RNA / Q000037:antagonists & inhibitors / Q000235:genetics; D012326:RNA Splicing; D013873:Thionucleotides / Q000494:pharmacology / Q000627:therapeutic use; D014777:Virus Diseases / Q000628:therapy
D016376:Oligonucleotides, Antisense; D013873:Thionucleotides; D012313:RNA