Cloning and expression of a myc family member from the pituitary gland of the Rainbow trout, Oncorhynchus mykiss.
A myc gene cloned from a Rainbow trout pituitary gland cDNA library is described. This clone (Tmyc2) shows extensive homology to Rainbow trout C-myc, which is expressed in the liver. Tmyc2 does not appear in the liver but is expressed in the pituitary gland (1.9 kb transcript), brain (2.0 kb transcript) and, at very low levels, in the heart (2.0 kb transcript). Tmyc2 contains three highly modified areas within the coding sequence, one of which shows an extensive loss of acidic residues that is uncommon in C-myc family members and may be important in determining the function of Tmyc2 in the pituitary gland and brain.
Panno
J P
JP
Department of Biological Sciences, Simon Fraser University, Burnaby, B.C., Canada.
McKeown
B A
BA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000378:metabolism; D015870:Gene Expression; D016259:Genes, myc; D008969:Molecular Sequence Data; D017683:Oncorhynchus / Q000235:genetics*; D010902:Pituitary Gland / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis
D018076:DNA, Complementary; D016271:Proto-Oncogene Proteins c-myc; D011994:Recombinant Proteins