Determination of ochratoxin A in bile of swine by high-performance liquid chromatography.
A method for the determination of low concentrations of the mycotoxin ochratoxin A (OA) in bile of pigs is described. OA was extracted with acidified chloroform and the extract was purified on a silicagel cartridge, followed by liquid-liquid partition. OA was determined by high-performance liquid chromatography with fluorescence detection. The detection limit was 0.3 ng/ml, the mean recovery was 84%. An enzyme-linked immunosorbent assay proved to be suitable for confirmation of positive results. Because of the low detection limit, biliary elimination of OA in pigs fed with low-contaminated feedstuffs can be examined by this method. This is an important requirement for the study of pharmacokinetic profiles of OA.
Kühn
I
I
Institute of Animal Nutrition, Federal Agricultural Research Centre Braunschweig-Völkenrode, Germany.
Valenta
H
H
Rohr
K
K
D000821:Animal Feed / Q000032:analysis; D000818:Animals; D001646:Bile / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D009793:Ochratoxins / Q000032:analysis* / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence; D013552:Swine
D009793:Ochratoxins; C025589:ochratoxin A