Characterization of acute homologous desensitization of mu-opioid receptor-induced currents in locus coeruleus neurones.
1. Acute homologous desensitization of mu-opioid receptor-induced currents was pharmacologically characterized in locus coeruleus (LC) neurones by use of intracellular and whole cell recording in superfused brain slices. 2. Following desensitization of opioid receptors by perfusion with a high concentration of [Met5] enkephalin (ME) for 5 min, there was a reduction in the maximum response and a rightward shift of the concentration-response curves for ME, [D-Ala2, N-MePhe4, Gly-ol]enkephalin (DAMGO) and normorphine. 3. By simultaneously fitting the operational model to the paired pre- and post-desensitization concentration-response data for each agonist, estimates of the level of desensitization were obtained. The values obtained for the three agonists (between 88% and 96%) were similar and did not vary according to the efficacy of the agonist used. 4. Use of whole cell patch recording techniques caused a slow rundown in the amplitude of ME currents (approx. 40% reduction over 60 min) but did not greatly affect the expression of acute desensitization of opioid currents. 5. When included in the patch recording solution, the phosphatase inhibitors, microcystin (50 nM-4 microM) and okadaic acid (1 microM) had no effect on the induction of desensitization or the normal ability of opioid or alpha 2-adrenoceptors to produce currents. Microcystin decreased the rate of recovery of the ME (300 nM) currents following desensitization; however, okadaic acid had little effect on the rate of recovery from desensitization. 6. Strong calcium buffering with BAPTA (10-20 mM) had no effect on desensitization or the recovery from desensitization. 6. Strong calcium buffering with BAPTA (10-20 mM) had no effect on desensitization or the recovery from desensitization.7 These results suggest that acute homologous desensitization of micro-opioid receptors in LC neurones entails a rapid loss of responsiveness that involves a majority of the receptor population. The mechanism by which desensitization is reversed may involve a non-calcium-dependent protein phosphatase but the processess that cause desensitization remain unclear.
Osborne
P B
PB
Vollum Institute, Oregon Health Sciences University, Portland 97201, USA.
Williams
J T
JT
D000818:Animals; D001921:Brain / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004361:Drug Tolerance; D004745:Enkephalins / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008125:Locus Coeruleus / Q000187:drug effects*; D008297:Male; D052998:Microcystins; D009022:Morphine Derivatives / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D010456:Peptides, Cyclic / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017450:Receptors, Opioid, mu / Q000187:drug effects*; D013997:Time Factors
D004745:Enkephalins; D004791:Enzyme Inhibitors; D052998:Microcystins; D009022:Morphine Derivatives; D010456:Peptides, Cyclic; D017450:Receptors, Opioid, mu; C078588:microcystin; C010616:normorphine