title: Attempted induction of blood brain barrier in vitro by co-culture of gerbil brain microvessel endothelial cells and glial cells.
abstract: The characteristics of isolated and subcultured Mongolian gerbil brain microvessel endothelial cells (BECs) were investigated, especially the disappearance of blood brain barrier (BBB) functions, and subcultured BECs were co-cultured with gerbil glial cells to assess a mass-producible BBB filter model. The subcultured cells lost specific characteristics observed in freshly isolated BECs, such as limited permeability to albumin and inulin, high gamma-glutamyl transpeptidase activity, and strong connections between individual cells. The subcultured BECs did not regain BBB functions during co-culture with glial cells, even derived from the same species. Only freshly isolated endothelial cells can be used for the BBB filter model, and a mass-producible BBB filter model may be impossible to make.
authors.affiliation: Department of Neurosurgery, Niigata University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Aiba
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000502:physiology*; D001921:Brain / Q000098:blood supply*; D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000166:cytology*; D005849:Gerbillinae; D008833:Microcirculation / Q000166:cytology; D009457:Neuroglia / Q000166:cytology*