Preliminary investigations of preconcentration-capillary electrophoresis-mass spectrometry.
Analyte preconcentration on-line with capillary electrophoresis-mass spectrometry (PC-CE-MS) is described. Preconcentration cartridges were fabricated from PTFE tubing filled with ca. 1-2 mm bed of reversed-phase C18 HPLC packing or polymeric reversed-phase beads. The particle size of the stationary phase was of larger dimension than the internal diameter of the CE capillary. Therefore, PC-CE capillaries were assembled without frit material and held together by friction. The wide applicability of on-line PC-CE-MS is demonstrated by the analysis of solutions containing peptides, proteins, and synthetic analogues of putative metabolites of the neuroleptic agent haloperidol.
tomlinson
A J
AJ
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA.
Braddock
W D
WD
Benson
L M
LM
Oda
R P
RP
Naylor
S
S
D000595:Amino Acid Sequence; D019075:Electrophoresis, Capillary / Q000379:methods*; D006220:Haloperidol / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis
D010455:Peptides; D011506:Proteins; D006220:Haloperidol