title: Dependence of peptide binding by MHC class I molecules on their interaction with TAP.
abstract: Major histocompatibility complex (MHC) class I molecules bind peptides that are delivered from the cytosol into the endoplasmic reticulum by the MHC-encoded transporter associated with antigen processing (TAP). Peptide capture by immature heterodimers of class I heavy chains and beta 2-microglobulin may be facilitated by their physical association with TAP. A genetic defect in a human mutant cell line causes the complete failure of diverse class I heterodimers to associate with TAP. This deficiency impairs the ability of the class I heterodimers to efficiently capture peptides and results from loss of function of an unidentified gene or genes linked to the MHC.
authors.affiliation: Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98104, USA.
authors.forename: A G
authors.initials: AG
authors.lastname: Grandea
authors.forename: M J
authors.initials: MJ
authors.lastname: Androlewicz
authors.forename: R S
authors.initials: RS
authors.lastname: Athwal
authors.forename: D E
authors.initials: DE
authors.lastname: Geraghty
authors.forename: T
authors.initials: T
authors.lastname: Spies
mesh_terms: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins / Q000378:metabolism; D037281:Calnexin; D002460:Cell Line; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006680:HLA Antigens / Q000378:metabolism; D015788:HLA-A1 Antigen / Q000378:metabolism; D015235:HLA-B Antigens / Q000378:metabolism; D015795:HLA-B8 Antigen / Q000378:metabolism; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D008285:Major Histocompatibility Complex / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D010455:Peptides / Q000378:metabolism*; D014162:Transfection; D001613:beta 2-Microglobulin / Q000378:metabolism
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D002135:Calcium-Binding Proteins; D006680:HLA Antigens; D015788:HLA-A1 Antigen; D015235:HLA-B Antigens; C035270:HLA-B5 antigen; D015795:HLA-B8 Antigen; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I; D008024:Ligands; D010455:Peptides; C073119:TAP1 protein, human; D001613:beta 2-Microglobulin; D037281:Calnexin; C071692:TAP2 protein, human