Involvement of folic acid in the synthesis of membrane-associated nucleotide sugars by normal and transformed mouse fibroblasts.
A plasma-membrane-containing fraction isolated from Swiss mouse (3T3) fibroblasts was found to contain uridine diphosphate sugars and methyl-uridine diphosphate (mUDP) sugars. The synthesis of the mUDP sugars by membrane preparations of normal and transformed 3T3 cells occurred from the intrinsic membrane uridine-diphosphate sugars + methylenetetrahydrofolic acid. Compared to the normal 3T3 cells, the synthesis of mUDP sugars by the transformed 3T3 cells was reduced by 49%. It is proposed that the plasma-membrane-associated mUDP sugars may be essential for normal cell growth.
Wood
R C
RC
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston 77555-1019, USA.
D016475:3T3 Cells; D000818:Animals; D002455:Cell Division; D002458:Cell Fractionation; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000378:metabolism*; D005492:Folic Acid / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D013763:Tetrahydrofolates / Q000378:metabolism; D014539:Uridine Diphosphate Sugars / Q000096:biosynthesis*
D009702:Nucleoside Diphosphate Sugars; D013763:Tetrahydrofolates; D014539:Uridine Diphosphate Sugars; C013123:5,10-methylenetetrahydrofolic acid; D005492:Folic Acid