title: Crystal structure of Bacillus licheniformis 1,3-1,4-beta-D-glucan 4-glucanohydrolase at 1.8 A resolution.
abstract: The crystal structure of the 1,3-1,4-beta-D-glucan 4-glucanohydrolase from Bacillus licheniformis is solved at a resolution of 1.8 A and refined to R = 16.5%. The protein has a similar beta-sandwich structure as the homologous enzyme from Bacillus macerans and the hybrid H(A16-M). This demonstrates that the jellyroll fold of these proteins is remarkably rigid and only weakly influenced by crystal contacts. The crystal structure permits to extend mechanistic considerations derived for the B. licheniformis enzyme to the entire class of bacterial 1,3-1,4-beta-D-glucan 4-glucanohydrolases.
authors.affiliation: Forschungsgruppe Kristallographie, Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Hahn
authors.forename: J
authors.initials: J
authors.lastname: Pons
authors.forename: A
authors.initials: A
authors.lastname: Planas
authors.forename: E
authors.initials: E
authors.lastname: Querol
authors.forename: U
authors.initials: U
authors.lastname: Heinemann
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001665:Binding Sites; D018360:Crystallography, X-Ray; D006026:Glycoside Hydrolases / Q000737:chemistry*; D011487:Protein Conformation
chemical_list: D006026:Glycoside Hydrolases; C028020:licheninase