STT3, a novel essential gene related to the PKC1/STT1 protein kinase pathway, is involved in protein glycosylation in yeast.
Mutations of genes involved in the STT1/PKC1 pathway in yeast show staurosporine and temperature sensitivities (stt) which are suppressed by the addition of 1 M sorbitol [Yoshida et al., Mol. Gen. Genet. 242 (1994) 631-640]. Among the stt mutants, stt3-2 shares this phenotype. The STT3 gene encodes a novel 718-amino-acid protein with significant homology to potential transmembrane proteins of Caenorhabditis elegans and mouse mandibular condyle (about 80% homologous and 60% identical). Unlike the STT1/PKC1 gene, STT3 is essential for cell growth irrespective of osmotic support. Pulse-chase experiments show that the sst3 mutants are defective in protein glycosylation. The stt3 mutants are sensitive to hygromycin B and resistant to sodium orthovanadate, whose phenotypes are common to those defective in protein glycosylation.
Yoshida
S
S
Department of Plant Sciences, University of Tokyo, Japan.
Ohya
Y
Y
Nakano
A
A
Anraku
Y
Y
D000470:Alkaloids / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D006031:Glycosylation; D006602:Hexosyltransferases; D006921:Hygromycin B / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009997:Osmotic Pressure; D011493:Protein Kinase C / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D019311:Staurosporine
D000470:Alkaloids; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; D006921:Hygromycin B; D006602:Hexosyltransferases; C096554:STT3 protein, S cerevisiae; D011493:Protein Kinase C; D019311:Staurosporine