title: Binding and phagocytosis of apoptotic vascular smooth muscle cells is mediated in part by exposure of phosphatidylserine.
abstract: Apoptosis of vascular smooth muscle cells has recently been demonstrated to occur in vitro and in vivo. Uptake of apoptotic cells into adjacent normal cells appears to be rapid and specific. We have investigated binding and phagocytosis of apoptotic vascular smooth muscle cells by normal smooth muscle cell monolayers. Vascular smooth muscle cells were infected with the proto-oncogene c-myc or the adenovirus E1A gene, induced to undergo apoptosis in low-serum conditions, and then incubated with normal smooth muscle cells. Apoptosis was accompanied by a marked increase in exposure of phosphatidylserine on the outer surface of the cell, which was recognized by binding to annexin V. Liposomes containing phosphatidylserine but not phosphatidylinositol inhibited uptake of apoptotic cells in a dose-dependent manner to a maximum of 50% inhibition; annexin V also inhibited the uptake of apoptotic cells in a dose-dependent and calcium-dependent manner. Binding of apoptotic bodies did not appear to be mediated by endogenous annexin V, as evidenced by the inability of an antibody to annexin V to inhibit uptake. Smooth muscle cells were also able to recognize exposed phosphatidylserine on other cell types, as judged by their ability to bind erythrocytes having a high degree of exposed phosphatidylserine. We conclude that smooth muscle cells express phosphatidylserine during apoptosis, and this exposure partly mediates binding and phagocytosis of dead cells. This mechanism may be important in promoting rapid cell removal in the vessel wall.
authors.affiliation: Department of Pathology, University of Washington, Seattle, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Bennett
authors.forename: D F
authors.initials: DF
authors.lastname: Gibson
authors.forename: S M
authors.initials: SM
authors.lastname: Schwartz
authors.forename: J F
authors.initials: JF
authors.lastname: Tait
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D017304:Annexin A5 / Q000276:immunology / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D004912:Erythrocytes / Q000378:metabolism; D016259:Genes, myc; D005822:Genetic Vectors; D008081:Liposomes; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D010587:Phagocytosis; D010718:Phosphatidylserines / Q000494:pharmacology*; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D012190:Retroviridae
chemical_list: D017304:Annexin A5; D008081:Liposomes; D010718:Phosphatidylserines; D004247:DNA