beta-1,3-Glucanase is highly-expressed in laticifers of Hevea brasiliensis.
Clones encoding beta-1,3-glucanase have been isolated from a Hevea cDNA library prepared from the latex of Hevea brasiliensis using a probe Nicotiana plumbaginifolia cDNA encoding beta-1,3-glucanase, gnl. Nucleotide sequence analysis showed that a 1.2 kb Hevea cDNA encoding a basic beta-1,3-glucanase showed 68% nucleotide homology to gnl cDNA. Northern blot analysis using the Hevea cDNA as probe detected a mRNA of 1.3 kb which was expressed at higher levels in latex than in leaf. In situ hybridization analysis using petiole sections from Hevea localized the beta-1,3-glucanase mRNA to the laticifer cells. Genomic Southern analysis suggested the presence of a low-copy gene family encoding beta-1,3-glucanases in H. brasiliensis.
Chye
M L
ML
Department of Botany, University of Hong Kong, Hong Kong.
Cheung
K Y
KY
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D043326:Glucan 1,3-beta-Glucosidase; D017403:In Situ Hybridization; D007840:Latex / Q000737:chemistry; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000166:cytology / Q000201:enzymology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014197:Trees / Q000033:anatomy & histology / Q000201:enzymology* / Q000235:genetics; D001617:beta-Glucosidase / Q000235:genetics* / Q000302:isolation & purification*
D015342:DNA Probes; D018076:DNA, Complementary; D007840:Latex; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase