title: Exposure of Drosophila melanogaster embryonic cell cultures to 60-Hz sinusoidal magnetic fields: assessment of potential teratogenic effects.
abstract: There is considerable concern about potential detrimental health effects associated with exposure to environmentally relevant magnetic fields. One specific concern relates to potential effects of magnetic field (MF) exposure on reproduction and development. Consequently, an in vitro teratogenesis (developmental toxicity) assay employing embryonic Drosophila cells has been used to determine whether exposure to a 60-Hz MF of 100 microT for 16-18 hr is itself teratogenic and whether such an exposure could potentiate the teratogenic response induced by a chemical teratogen (developmental toxicant). The results demonstrated that (1) MF exposure alone did not induce a teratogenic response, whether the MF was oriented parallel or perpendicular to the plane of the culture dishes; and (2) MF exposure did not alter the teratogenic response induced by optimal or suboptimal concentrations of three chemical teratogens (retinoic acid, hydroxyurea, and cadmium). Furthermore, in additional studies, Drosophila embryos were exposed to 60-Hz MFs of 10 and 100 microT for 24 hr or for their entire development time (i.e., until adult ecolsion, about 10 days). Results demonstrated that MF exposure did not produce an increase in developmental abnormalities over those observed in unexposed controls.
authors.affiliation: California State University San Bernardino, Department of Biology 92407, USA.
authors.forename: P
authors.initials: P
authors.lastname: Nguyen
authors.forename: N
authors.initials: N
authors.lastname: Bournias-Vardiabasis
authors.forename: W
authors.initials: W
authors.lastname: Haggren
authors.forename: W R
authors.initials: WR
authors.lastname: Adey
authors.forename: J L
authors.initials: JL
authors.lastname: Phillips
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity; D002478:Cells, Cultured; D004331:Drosophila melanogaster / Q000196:embryology* / Q000528:radiation effects*; D004574:Electromagnetic Fields / Q000009:adverse effects*; D017952:Ganglia, Invertebrate / Q000528:radiation effects; D006918:Hydroxyurea / Q000633:toxicity; D018485:Muscle Fibers, Skeletal / Q000528:radiation effects; D013723:Teratogens / Q000633:toxicity*; D014212:Tretinoin / Q000633:toxicity
chemical_list: D013723:Teratogens; D002104:Cadmium; D014212:Tretinoin; D006918:Hydroxyurea