A mRNA variant encoding a soluble form of 4-1BB, a member of the murine NGF/TNF receptor family.
The murine 4-1BB gene, encoding a member of the nerve growth factor/tumor necrosis factor (NGF/TNF) receptor family, is thought to be selectively expressed in T cells and is involved in the regulation of lymphocyte proliferation. We detected two forms of the 4-1BB mRNA by RT-PCR which were expressed in an activation-dependent pattern in splenocytes and thymocytes. cDNA sequencing showed that the smaller form was a mRNA splice variant lacking the transmembrane region (4-1BB delta TM), because of the deletion of exon 8. The two forms of mRNA are differentially expressed in murine T cells, macrophages, 3T3 fibroblasts and epitheloid cells. Northern blotting also identifies two forms of mRNA of 1.5 and 2.4 kb, and the cell-type-specific pattern correlated with the PCR results. These results identify a novel form of 4-1BB. This and the previously known membrane-associated form have a broad tissue distribution, suggesting a more diverse role in host defense.
Setareh
M
M
Sam and Rose Stein Institute for Research on Aging, University of California at San Diego, La Jolla 92093-0663, USA.
Schwarz
H
H
Lotz
M
M
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D001483:Base Sequence; D015152:Blotting, Northern; D017931:DNA Primers; D005091:Exons; D015870:Gene Expression; D014644:Genetic Variation; D008213:Lymphocyte Activation; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis*; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics*; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis* / Q000235:genetics*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9
D015703:Antigens, CD; D017931:DNA Primers; D021382:Protein Sorting Signals; D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor; D018124:Receptors, Tumor Necrosis Factor; C505715:Tnfrsf9 protein, mouse; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9