Complete sequence of an infectious full-length cDNA clone of citrus tatter leaf capillovirus: comparative sequence analysis of capillovirus genomes.
The complete nucleotide sequence of citrus tatter leaf capillovirus (CTLV lily strain) was determined. It is 6496 nucleotides long, excluding the 3'-terminal poly(A) tract, and contains two putative overlapping open reading frames (ORFs). ORF1 (positions 37-6354) encodes a potential polyprotein of molecular mass 242 kDa. ORF2 (positions 4788-5750) codes for a 36 kDa protein. The 242 kDa polypeptide contains several non-structural protein domains (i.e. methyltransferase, NTP-binding helicase, papain-like proteinase and polymerase) and, at its C terminus, the putative coat protein. The N-terminal region of the 36 kDa protein displays sequence similarity to the cell-to-cell movement proteins of the '30 K superfamily'. Such a genome structure is conserved between CTLV and apple stem grooving capillovirus. Capped transcripts from a plasmid containing the complete sequence of CTLV, with a T7 RNA promoter, successfully infected Chenopodium quinoa plants and caused symptoms characteristic of CTLV. Uncapped transcripts were noninfectious.
Ohira
K
K
Laboratory of Bioresource Technology, Graduate School, University of Tokyo, Japan.
Namba
S
S
Rozanov
M
M
Kusumi
T
T
Tsuchizaki
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D002957:Citrus / Q000821:virology*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004279:DNA, Viral; D016679:Genome, Viral; D008780:Methyltransferases / Q000032:analysis; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000032:analysis
D018076:DNA, Complementary; D004279:DNA, Viral; D014764:Viral Proteins; D008780:Methyltransferases