A mutant of pseudorabies virus with deletion of glycoprotein gIII gene prepared from a Japanese isolate: it fails to agglutinate mouse erythrocytes.
A mutant with deletion of the glycoprotein gIII gene was produced from a Japanese isolate of pseudorabies virus (PrV) and characterized. Viral titers of the mutant propagated in PK-15 cells were always lower than those of the parental virus. The parental virus agglutinated BALB/c mouse erythrocytes, whereas the deletion mutant showed no hemagglutinating activity. Pigs inoculated with the parental virus produced not only neutralizing but also hemagglutination-inhibiting antibodies. On the other hand, the mutant induced high titers of neutralizing antibody comparable to the parental virus but no hemagglutination-inhibiting antibody in inoculated pigs, suggesting that glycoprotein gIII is an essential component for hemagglutination of PrV. Finally, no evidence that the deletion mutant lost virulence for mice was obtained.
Yamada
S
S
Second Research Division, National Institute of Animal Health, Ibaraki, Japan.
Imada
T
T
Shimizu
M
M
Miura
Y
Y
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D017353:Gene Deletion; D006387:Hemagglutination, Viral; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D007114:Immunization; D007564:Japan; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D011557:Pseudorabies / Q000097:blood / Q000276:immunology / Q000517:prevention & control; D013552:Swine; D014759:Viral Envelope Proteins / Q000235:genetics*; D014774:Virulence
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C042966:pseudorabies virus glycoproteins