title: Eicosanoids are produced by microglia, not by astrocytes, in rat glial cell cultures.
abstract: To determine principal sources of eicosanoid production in glial cells, we analyzed the metabolites of arachidonic acid in cultured rat glial cells by use of reversed-phase, high-performance liquid chromatography and an on-line radioisotope detector. Prostaglandin D2, leukotriene B4, leukotriene C4, and 5-hydroxyeicosatetraenoic acid were present in cultures in which microglia appeared on a monolayer astrocytes. None were detected in culture dishes that contained only astrocytes, although astrocytes have been believed to be a main source of eicosanoid production in brain.
authors.affiliation: Department of Pediatrics, Saga Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsuo
authors.forename: Y
authors.initials: Y
authors.lastname: Hamasaki
authors.forename: F
authors.initials: F
authors.lastname: Fujiyama
authors.forename: S
authors.initials: S
authors.lastname: Miyazaki
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism*; D001253:Astrocytes / Q000378:metabolism*; D002478:Cells, Cultured; D005069:Evaluation Studies as Topic; D017628:Microglia / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016718:Arachidonic Acid