Characterization and structural organization of the cardiac beta-myosin heavy chain gene from Syrian hamster.
In order to perform comparative studies of the cardiac myosin heavy chain (MyHC) genes, we determined the sequence of the Syrian hamster beta-MyHC gene and its 5' flanking region. This 33,960 basepair (bp) sequence contains 12,196 bp of the 5' flanking region as well as 21,731 bp of the complete beta-MyHC gene, with its 3' end overlapping with the alpha-MyHC gene. All the exon/intron boundaries were determined and relative to the human beta-MyHC gene, an extra 5' untranslated exon was identified. The isolation and sequencing of the Syrian hamster beta-MyHC gene may further the understanding about the regulation and the evolution of the cardiac MyHC genes.
Wang
R
R
Laboratory for Molecular Cardiology, Toronto Hospital, Canada.
Sole
M J
MJ
Cai
X
X
Liew
C C
CC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D005091:Exons; D005796:Genes; D008647:Mesocricetus / Q000235:genetics*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D018995:Myosin Heavy Chains