Repair of plasmid and genomic DNA in a rad7 delta mutant of yeast.
Repair of UV-induced cyclobutane pyrimidine dimers (CPDs) was examined in a yeast plasmid of known chromatin structure and in genomic DNA in a radiation-sensitive deletion mutant of yeast, rad7 delta, and its isogenic wild-type strain. A whole plasmid repair assay revealed that only approximately 50% of the CPDs in plasmid DNA are repaired after 6 h in this mutant, compared with almost 90% repaired in wild-type. Using a site-specific repair assay on 44 individual CPD sites within the plasmid we found that repair in the rad7 delta mutant occurred primarily in the transcribed regions of each strand of the plasmid, however, the rate of repair at nearly all sites measured was less than in the wild-type. There was no apparent correlation between repair rate and nucleosome position. In addition, approximately 55% of the CPDs in genomic DNA of the mutant are repaired during the 6 h period, compared with > 80% in the wild-type.
Mueller
J P
JP
Department of Biochemistry and Biophysics, Washington State University, Pullman 99164-4660, USA.
Smerdon
M J
MJ
D002843:Chromatin / Q000648:ultrastructure; D004249:DNA Damage / Q000528:radiation effects; D004260:DNA Repair; D004271:DNA, Fungal / Q000235:genetics* / Q000528:radiation effects; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics*; D010957:Plasmids; D011740:Pyrimidine Dimers; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014158:Transcription, Genetic; D014466:Ultraviolet Rays
D002843:Chromatin; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D011740:Pyrimidine Dimers; C063915:RAD7 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins