title: Separation of cyclodextrins and their derivatives by thin-layer and preparative column chromatography.
abstract: Cyclodextrins, their derivatives and products of their partial hydrolysis are clearly separated by chromatography on silica gel by mobile phases containing aqueous ammonia and organic solvent (acetonitrile or 1-propanol). This system allows separation of compounds with different numbers of substituents on cyclodextrin, but does not allow the separation of isomers; the system can be used both for thin-layer chromatography and for preparative column chromatography. The isomers in the fraction thus obtained can be in some cases separated, after peracetylation, by chromatography on silica gel using a mobile phase consisting of methanol and dichloromethane. This chromatographic sequence potentially yields derivatives of cyclodextrins in quantities which enable evaluation of their solubilization and biological properties.
authors.affiliation: National Institutes of Health, NIA/GRC, Baltimore, MD 21224, USA.
authors.forename: J
authors.initials: J
authors.lastname: Jindrich
authors.forename: J
authors.initials: J
authors.lastname: Pitha
authors.forename: B
authors.initials: B
authors.lastname: Lindberg
mesh_terms: D000107:Acetylation; D002845:Chromatography; D002855:Chromatography, Thin Layer; D003505:Cyclodextrins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D006868:Hydrolysis; D013058:Mass Spectrometry; D009005:Monosaccharides / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis; D012997:Solvents
chemical_list: D003505:Cyclodextrins; D009005:Monosaccharides; D009844:Oligosaccharides; D012997:Solvents