[Development of a method of obtaining lyophilized antileukocyte histotyping sera intended for long-term storage].
An optimal scheme for sublimation of anti-HLA-sera of locuses A, B, C, and DR has been developed, consisting of freezing at 60 degrees C and lyophilization to 29-30 degrees C, this permitting the preparation of initially active antileukocytic sera. The activity and specificity of defrosted native and lyophilized anti-HLA-sera were assessed in the complement-dependent cytotoxicity test. Four-year follow-up of the activity of dried anti-HLA-sera showed that the stabilizer was not needed, for the activities of the sera lyophilized with and without it was virtually equally high. The optimal storage temperature for dry anti-HLA-sera is 4 degrees C.
Ivanova
R P
RP
Kochetkova
G A
GA
Sivakova
N P
NP
Bubnova
L N
LN
Khoroshaĭlova
A E
AE
D003601:Cytotoxicity Tests, Immunologic; D005500:Follow-Up Studies; D005612:Freeze Drying; D005615:Freezing; D006680:HLA Antigens / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D007106:Immune Sera; D007962:Leukocytes / Q000276:immunology*; D011309:Preservation, Biological; D013696:Temperature; D013997:Time Factors
D006680:HLA Antigens; D007106:Immune Sera
Razrabotka metoda polucheniia sukhikh antileĭkotsitarnykh gistotipiruiushchikh syvorotok s tsel'iu ikh dolgosrochnogo khraneniia.