P450aldo in hamster adrenal cortex: immunofluorescent and immuno-gold electron microscopic studies.
The zonal distribution of aldosterone synthase cytochrome P450 (P450aldo) in the adrenal cortex of male hamsters was investigated by immunofluorescence and electron microscopy, using an anti-P450aldo peptide antibody. On cryostat sections the immunolocalization of P450aldo was confined to the zona glomerulosa cells. On semi-thin plastic sections, P450aldo was shown to be located in mitochondria. Studies in electron microscopy, using the colloidal gold technique, confirmed that P450aldo was located in mitochondria.
LeHoux
J G
JG
Department of Biochemistry, Faculty of Medicine, Sherbrooke University, QC, Canada.
Martel
D
D
LeHoux
J
J
Ducharme
L
L
Lefebvre
A
A
Brière
N
N
D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D006224:Cricetinae; D019405:Cytochrome P-450 CYP11B2; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D017979:Gold Colloid; D008297:Male; D008647:Mesocricetus; D016253:Microscopy, Immunoelectron; D013252:Steroid 11-beta-Hydroxylase / Q000032:analysis*
D017979:Gold Colloid; D003577:Cytochrome P-450 Enzyme System; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase