Impaired tumor phenotypes in class II major histocompatibility complex antigen-inducible cells originated from human lung adenocarcinoma.
The class II major histocompatibility complex (MHC) antigens play important roles in T cell activation and are thought to be involved in tumor development. Using anti-class II antibodies with interferon gamma (IFN-gamma), we have here selected class II MHC antigen positive cancer cells from the human lung adenocarcinoma cell line A549, which is originally negative (> 95%) for the antigens. A part of the class II antigen-positive cells presented a flat morphology, which was not observed in the parental A549 cells. Class II antigen expression in these flat cells was IFN-gamma inducible; there was a correlation between the inducibility and phenotypic changes. A class II antigen-inducible flat subline restored the ability of contact inhibition and anchorage-dependent growth as well as lost tumorigenicity in athymic mice.
Kawamoto
S
S
Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.
Inoue
Y
Y
Shinozaki
Y
Y
Katakura
Y
Y
Tachibana
H
H
Shirahata
S
S
Murakami
H
H
D000230:Adenocarcinoma / Q000276:immunology*; D000818:Animals; D005434:Flow Cytometry; D000949:Histocompatibility Antigens Class II / Q000032:analysis* / Q000502:physiology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008175:Lung Neoplasms / Q000276:immunology*; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010641:Phenotype; D014407:Tumor Cells, Cultured
D000949:Histocompatibility Antigens Class II; D007371:Interferon-gamma