title: GCAP-II: isolation and characterization of the circulating form of human uroguanylin.
abstract: The systematic isolation of circulating regulatory peptides which generate cGMP as second messenger resulted in the identification of a novel member of the guanylin family. In the present study we describe the purification and amino acid sequence of a new guanylate cyclase C activating peptide (GCAP-II). GCAP-II contains 24 amino acids in the following sequence: FKTLRTIANDDCELCVNVACTGCL. Its molecular mass is 2597.7 Da. The 16 C-terminal amino acids are identical to uroguanylin from human urine. native and synthetic GCAP-II activate GC-C, the specific guanylate cyclase receptor, of cultured human colon carcinoma (T84) cells. GCAP-II stimulates chloride secretion in isolated human intestinal mucosa mediated by intracellular cGMP increase. GCAP-II specific antibodies were used to localize the peptide by immunohistochemistry in entero-endocrine cells of the colonic mucosa.
authors.affiliation: Lower Saxony Institute for Peptide Research (IPF), Hannover, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Hess
authors.forename: M
authors.initials: M
authors.lastname: Kuhn
authors.forename: P
authors.initials: P
authors.lastname: Schulz-Knappe
authors.forename: M
authors.initials: M
authors.lastname: Raida
authors.forename: M
authors.initials: M
authors.lastname: Fuchs
authors.forename: J
authors.initials: J
authors.lastname: Klodt
authors.forename: K
authors.initials: K
authors.lastname: Adermann
authors.forename: V
authors.initials: V
authors.lastname: Kaever
authors.forename: Y
authors.initials: Y
authors.lastname: Cetin
authors.forename: W G
authors.initials: WG
authors.lastname: Forssmann
mesh_terms: D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification* / Q000502:physiology; D006152:Cyclic GMP / Q000378:metabolism; D051604:Guanylate Cyclase-Activating Proteins; D006801:Humans; D008969:Molecular Sequence Data; D045265:Natriuretic Peptides; D010455:Peptides / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
chemical_list: D002135:Calcium-Binding Proteins; C496739:GUCA1B protein, human; D051604:Guanylate Cyclase-Activating Proteins; D045265:Natriuretic Peptides; D010455:Peptides; C084362:uroguanylin; D006152:Cyclic GMP