Modulation of somatocardiac sympathetic reflexes mediated by opioid receptors at the spinal and brainstem level.
Modulation of somatosympathetic reflexes at the spinal cord and the brainstem was studied by administering opioid receptor agonists into the intrathecal space of the lumbar spinal cord and into the subarachnoid space of the cisterna magna in rats anesthetized with alpha-chloralose and urethane. Somatocardiac sympathetic A- and C-reflexes were elicited by electrical stimulation of myelinated (A) and unmyelinated (C) afferent fibers of the tibial nerve, respectively. Intrathecal administration of the mu-opioid receptor agonist DAMGO selectively depressed the C-reflex in a dose-dependent manner (minimum effective dose 10 ng), whereas the intrathecal injection of the delta-opioid receptor agonist DPDPE and the kappa-opioid receptor agonist U-50,488H only at doses of 10 micrograms and 100 micrograms, respectively, led to a significant depression of the C-reflex. Injection of DAMGO into the cisterna magna enhanced both A- and C-reflexes in a dose-dependent manner (minimum effective dose 1 ng). The administration of neither DPDPE nor U-50,488H into the cisterna magna affected A- or C-reflexes. It is concluded that the activation of mu-opioid receptors is mainly or exclusively responsible for suppressing somatosympathetic C-reflexes at the spinal cord and for enhancing them at the brainstem.
Sato
A
A
Department of the Autonomic Nervous System, Tokyo Metropolitan Institute of Gerontology, Japan.
Sato
Y
Y
Schmidt
R F
RF
D000320:Adrenergic Fibers / Q000187:drug effects*; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D001933:Brain Stem / Q000187:drug effects*; D002946:Cisterna Magna / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D004745:Enkephalins / Q000494:pharmacology*; D007278:Injections, Spinal; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D017450:Receptors, Opioid, mu / Q000819:agonists* / Q000187:drug effects*; D012018:Reflex / Q000187:drug effects*; D013116:Spinal Cord / Q000187:drug effects*
D000700:Analgesics; D004745:Enkephalins; D017450:Receptors, Opioid, mu; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-