Photochemical linkage of antibodies to silicon chips.
Antibodies and antigen binding fragments thereof were photochemically immobilized on surface-modified silicon chips of 5 x 5 mm size. Silicon surface-grafted diazirines and benzophenones formed covalent bonds with the immunoreagents on light activation. Photolithographic immobilization of monoclonal antibodies in aqueous media was achieved on silicon chips by activating surface-grafted benzophenones. The presence of bovine serum albumin during irradiation reduced nonspecific adsorption of the immunoreagents and retained the immunoactivity of the photoimmobilized molecules.
Sundarababu
G
G
Institute of Biochemistry, University of Bern, Switzerland.
Gao
H
H
Sigrist
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D051379:Mice; D010777:Photochemistry / Q000379:methods; D012825:Silicon / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D012825:Silicon