title: Clonal expansion of T cells and HIV genotypes in microdissected splenic white pulps indicates viral replication in situ and infiltration of HIV-specific cytotoxic T lymphocytes.
abstract: Human immunodeficiency virus (HIV) replication and T cell proliferation was investigated in situ by a PCR based analysis of individual microdissected splenic white pulps. Founder effects, revealed by an exquisite compartmentalization of HIV genotypes and T cells, indicated the recruitment of latently infected CD4+ T cells through highly localized antigen presentation, rather than the infection of CD4+ T lymphoblasts by blood borne virus or immune complexes. HIV infected white pulps could be infiltrated by HIV specific cytotoxic T lymphocytes, so implicating them in CD4+ T cell destruction in vivo. Together these data describe an iterative and deleterious mechanism of antigen driven T cell recruitment and activation, HIV replication and spread, with consequent destruction of the newly infected cells.
authors.affiliation: Unité de Rétrovirologie Moléculaire, Institut Pasteur, Paris, France.
authors.forename: R
authors.initials: R
authors.lastname: Cheynier
authors.forename: S
authors.initials: S
authors.lastname: Henrichwark
authors.forename: F
authors.initials: F
authors.lastname: Hadida
authors.forename: E
authors.initials: E
authors.lastname: Pelletier
authors.forename: E
authors.initials: E
authors.lastname: Oksenhendler
authors.forename: B
authors.initials: B
authors.lastname: Autran
authors.forename: S
authors.initials: S
authors.lastname: Wain-Hobson
mesh_terms: D000595:Amino Acid Sequence; D000918:Antibody Specificity; D002999:Clone Cells; D004210:Dissection; D005838:Genotype; D006678:HIV / Q000235:genetics / Q000502:physiology*; D015483:HIV Antibodies; D006801:Humans; D008846:Micromanipulation; D008969:Molecular Sequence Data; D013154:Spleen / Q000473:pathology*; D013602:T-Lymphocytes, Cytotoxic; D014779:Virus Replication
chemical_list: D015483:HIV Antibodies