Augmentation of murine hematopoiesis by interleukin 2-activated irradiated T cells.
We have examined the role of T cells activated with interleukin-2 (IL-2) in vitro and subsequently irradiated (2500 rads), in stimulating murine hematopoiesis in a syngeneic system. Our data suggest that activated, irradiated T (AIT) cells significantly increased the progenitor cell activity of T cell-depleted bone marrow (BM) both in vitro and in vivo as compared with controls (P < 0.001). The efficacy of AIT cells was comparable to that of activated, nonirradiated T (AT) cells (P > 0.05). Optimal stimulation of BM progenitor cell activity was seen when T cells were activated for 4 days and used in a BM to T cell ratio of 1:2 or 1:5. The effect of these activated cells was related to the release of factors with ability to enhance hematopoiesis. These observations may have implications in enhancing the engraftment of T cell-depleted BM in allogeneic transplantation.
Charak
B S
BS
Bone Marrow Transplantation Program, Georgetown University Medical Center, Washington, D.C. 20007, USA.
Brown
E G
EG
Mazumder
A
A
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D005260:Female; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D066298:In Vitro Techniques; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D011839:Radiation, Ionizing; D013601:T-Lymphocytes / Q000502:physiology / Q000528:radiation effects*
D007376:Interleukin-2