Decreased phospholipid polyunsaturated fatty acid content and superoxide dismutase activity in cardiac muscle of malignant hyperthermia-susceptible swine.
Homogenates of cardiac left ventricle from malignant hyperthermia-susceptible (MHS) pigs produced a circa 72% more pentane than those from malignant hyperthermia-resistant (MHR) animals, indicating enhanced peroxidation of n-6 fatty acids. This is consistent with the observed circa 70% decrease in total phospholipid polyunsaturated fatty acids (PUFA) in MHS compared with MHR tissue, a decrease mainly due to the quantitatively greater loss of n-6 PUFA. Although the percentage loss of n-3 PUFA was greater than that of n-6 PUFA (90% vs 60%), absolute amounts were insufficient to register as ethane production. Three-fold greater phospholipid content of MHS compared with MHR ventricles indicates reduced neutral lipid content probably due to increased catecholamine stimulation. These findings were associated with a small but significant decrease in superoxide dismutase activity in MHS tissues.
Wahle
K W
KW
Rowett Research Institute, Aberdeen, Scotland, United Kingdom.
Morrice
P C
PC
Arthur
J R
JR
Duthie
G G
GG
D000818:Animals; D000975:Antioxidants / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D008055:Lipids / Q000032:analysis; D008305:Malignant Hyperthermia / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D012643:Selenium / Q000032:analysis; D013482:Superoxide Dismutase / Q000032:analysis*; D013552:Swine; D014810:Vitamin E / Q000032:analysis
D000975:Antioxidants; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D014810:Vitamin E; D013482:Superoxide Dismutase; D012643:Selenium