title: Specific inhibition of vascular cell adhesion molecule-1 expression by type IV collagen in endothelial cells.
abstract: The effects of matrices on the expression of adhesion molecules were studied in cultured human umbilical cord vein endothelial cells(HUVEC). The expression of vascular cell adhesion molecule-1 (VCAM-1) but not of intercellular adhesion molecule-1 stimulated with various substances was remarkably low in HUVEC cultured on type IV collagen, the major component of the basement membrane, compared with that on type I collagen. This inhibition was observed not only at the protein level but also at the mRNA level. These data suggested that VCAM-1 expression in endothelial cells is strictly regulated by the underlying basement membrane.
authors.affiliation: Second Department of Internal Medicine, School of Medicine, Chiba University, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Morisaki
authors.forename: T
authors.initials: T
authors.lastname: Kanzaki
authors.forename: K
authors.initials: K
authors.lastname: Tamura
authors.forename: I
authors.initials: I
authors.lastname: Saito
authors.forename: R
authors.initials: R
authors.lastname: Shiina
authors.forename: Y
authors.initials: Y
authors.lastname: Saito
mesh_terms: D001485:Basement Membrane / Q000502:physiology; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression / Q000187:drug effects*; D005786:Gene Expression Regulation; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology; D015847:Interleukin-4 / Q000494:pharmacology; D007700:Kinetics; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects; D014471:Umbilical Veins; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis*
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D011994:Recombinant Proteins; D019010:Vascular Cell Adhesion Molecule-1; D015847:Interleukin-4; D003094:Collagen