[The effect of ionizing radiation and physical loading on the molecular heterogeneity of nucleoside phosphate kinases in liver subcellular fractions].
The different molecular forms of nucleoside-monophosphate kinases (KF 2.7.4.4) and nucleoside-diphosphate kinases (KF 1.7.4.6) which are responsible for the final steps of pyrimidine nucleotide synthesis were determined in mitochondrial rat hepatic supernatant under condition of combined influence of X-ray and maximum physical exercises (running up to complete exhaustion). The maximum activity of investigated nucleosidediphosphate kinases were observed in intact animals in that fractions which were eluted with tris-HCl buffer solution (0.075 and 1.0 M, pH 7.4). X-ray radiation and physical exercises caused the deviation of chromatographic data of maximal enzymatic activities. The drastic lowering of investigated enzymes was observed after X-ray irradiation and maximum physical exhaustion. This fact is in favour for the suppression of the final steps of primidine nucleotides synthesis under explored conditions of experimental investigations.
Nagiev
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D000818:Animals; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D005720:Gamma Rays; D018740:Genetic Heterogeneity / Q000528:radiation effects; D008099:Liver / Q000737:chemistry / Q000201:enzymology* / Q000528:radiation effects*; D008297:Male; D009703:Nucleoside-Phosphate Kinase / Q000032:analysis / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D005082:Physical Exertion / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000737:chemistry / Q000201:enzymology / Q000528:radiation effects
D009703:Nucleoside-Phosphate Kinase
Vliianie ioniziruiushcheĭ radiatsii i fizicheskoĭ nagruzki na molekuliarnuiu geterogennost' nukleozidfosfatkinaz v subkletochnykh fraktsiiakh pecheni.