Effect of selenium on malignant tumor cells of brain.
Some reports have demonstrated that selenium can inhibit tumorigenesis in some tissues of animal. However, little is known about the inhibitory effect on malignant tumor cells of brain. The purpose of our study was to determine the biological effect of selenium on growth of rat glioma and human glioblastoma cell lines. Cell lines C6 and A172 were obtained from Japanese Cancer Research Resources Bank, Tokyo, Japan (JCRB). Cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal calf serum at 37 degrees C in a humidified atmosphere of air and 5% CO2. Antiproliferative effects of selenium were evaluated using growth rate assay quantifying cell number by MTT assay. An antiproliferative effect of selenium was found in two cell lines, which was more effective on human A172 glioblastoma and less effective on rat C6 glioma.
Zhu
Z
Z
Department of Hygiene, Faculty of Medicine, Kyoto University, Japan.
Kimura
M
M
Itokawa
Y
Y
Nakatsu
S
S
Oda
Y
Y
Kikuchi
H
H
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000473:pathology / Q000517:prevention & control*; D016923:Cell Death / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D005909:Glioblastoma / Q000473:pathology / Q000517:prevention & control*; D005910:Glioma / Q000473:pathology / Q000517:prevention & control*; D006801:Humans; D051381:Rats; D012643:Selenium / Q000494:pharmacology*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured / Q000187:drug effects
D016588:Anticarcinogenic Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue; D012643:Selenium