In vitro synthesis of novel genes: mutagenesis and recombination by PCR.
Vallejo
A N
AN
Department of Immunology, Mayo Clinic/Foundation, Rochester, Minnesota 55905, USA.
Pogulis
R J
RJ
Pease
L R
LR
D004247:DNA / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase; D004587:Electrophoresis, Agar Gel; D005813:Genes, Synthetic; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis / Q000276:immunology; D006801:Humans; D007202:Indicators and Reagents; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology; D011995:Recombination, Genetic; D019914:Taq Polymerase
D017931:DNA Primers; D015395:Histocompatibility Antigens Class I; D007202:Indicators and Reagents; D011994:Recombinant Proteins; D004247:DNA; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase