Effect of heparin on hemagglutination by Akabane and Aino viruses belonging to the Simbu group of bunyaviruses.
Heparin inhibited the hemagglutinin activity of Akabane and Aino viruses. The minimal inhibitory concentration of heparin required to inhibit 8 hemagglutination (HA) U of Akabane and Aino viruses was 10 U/ml. Goose erythrocytes failed to combine with the HA inhibitory factor of heparin. On the other hand, goose erythrocytes treated with heparinase had greatly reduced agglutinability by Akabane virus. Virus-heparin complex formation was observed by sedimenting heparin with the virus particles.
Jusa
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ER
Department of Veterinary Epizootiology, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
Inaba
Y
Y
Ishibashi
K
K
Noda
M
M
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D002043:Bunyaviridae / Q000187:drug effects* / Q000502:physiology; D002498:Centrifugation; D005777:Geese; D006387:Hemagglutination, Viral / Q000187:drug effects*; D006493:Heparin / Q000494:pharmacology*; D019763:Heparin Lyase; D051379:Mice; D011133:Polysaccharide-Lyases / Q000494:pharmacology
D000925:Anticoagulants; D006493:Heparin; D011133:Polysaccharide-Lyases; D019763:Heparin Lyase