[Clinical experience using the cryoprotector "hecmolit" in performing autologous bone marrow transplantation].
The authors summarize clinical evidence obtained on the efficacy of a new Russian cryoprotector hecmolit in the conduction of autotransplantation of 8 patients with acute leukemia. The markers of the preserved hemopoietic potential of the unfrozen bone marrow were the amount of myelokaryocytes, their viability in the test with vital stain, CFU-GM level. Infusions of the marrow suspension incorporating hecmolit (120-180 ml) produced minimaL side effects. After thawing myelokaryocytes remained in the amount 81.7 +/- 8.2% or 1.6 +/- 0.4 x 10(8) cell / kg, on the average. Estimation of the aggregates in terms of the weight revealed that hemopoietic potential was sufficient for the transplant survival (2.9 +/- 0.7 x 10(3) CFU-GM/kg) though only 50 +/- 10% of CFU-GM remained safe. Hecmolit tolerance was satisfactory. The preparation is thought convenient for bone marrow transplantation practice.
Abdulkadyrov
K M
KM
Moiseev
S I
SI
Ganapiev
A A
AA
Gritsaev
S V
SV
Balashova
V A
VA
Golubev
A E
AE
D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation; D003451:Cryoprotective Agents / Q000627:therapeutic use*; D006801:Humans; D009930:Organic Chemicals; D014182:Transplantation, Autologous
D003451:Cryoprotective Agents; D009930:Organic Chemicals; C096681:hecmolit
Opyt klinicheskogo primeneniia krioprotektora "gekmolit" pri provedenii autologichnoĭ transplantatsii kostnogo mozga.