High-performance capillary electrophoretic separation of proteins and peptides using a bonded hydrophilic phase capillary.
High performance capillary electrophoresis (HPCE) was applied to the separation of protein and peptide mixtures with molecular masses ranging from 1300 to 96000 Da using a new bonded hydrophilic phase capillary, CElect-P150. This coated capillary reduces the interaction between proteins and silanol groups in capillary walls, allowing a complete recovery of the proteins and peptides of interest. HPCE was also used for the analysis of a complex mixture of tryptic fragments and to monitor the process of enzymatic digestion. Moreover, using a CElect-P150 capillary, highly reproducible analysis was possible without preconditioning the capillary with acid or basic solutions before each new analysis.
Piccoli
G
G
Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, Italy.
Fiorani
M
M
Biagiarelli
B
B
Palma
F
F
Vallorani
L
L
De Bellis
R
R
Stocchi
V
V
D000818:Animals; D002417:Cattle; D003574:Cytochrome c Group / Q000737:chemistry / Q000378:metabolism; D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D014357:Trypsin
D003574:Cytochrome c Group; D010455:Peptides; D011506:Proteins; D012710:Serum Albumin, Bovine; D014357:Trypsin