title: Genetic map of 16 polymorphic markers forming three linkage groups assigned to rat chromosome 4.
abstract: Sixteen polymorphic markers, including markers for eight new loci, forming three linkage groups, were assigned to rat Chromosome (Chr) 4 by linkage analysis of the progeny of an F2 intercross of Fischer (F344/N) and Lewis (LEW/N) inbred rats. One gene, Igk, was mapped by restriction fragment length polymorphism (RFLP) analysis. One marker for Tcrb was identified by the polymorphic insertion of a repetitive LINE element. The remaining 14 markers contained polymorphic simple sequence repeats (SSRs). Ten were identified in genes (Tgfa, Npy, Prss1, Prss2, Aldr1, Iapp, Prp, Eno2, Cacnl1a1, and Il6), one was identified in a sequence related to a gene (Egr4l1), and three were identified in anonymous DNA segments. The SSR markers were highly polymorphic in 16 inbred rat strains. These markers expand the genetic map of the rat and should be useful in future genetic studies of inbred rats.
authors.affiliation: Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: E A
authors.initials: EA
authors.lastname: Goldmuntz
authors.forename: E F
authors.initials: EF
authors.lastname: Remmers
authors.forename: Y
authors.initials: Y
authors.lastname: Du
authors.forename: H
authors.initials: H
authors.lastname: Zha
authors.forename: P
authors.initials: P
authors.lastname: Mathern
authors.forename: L J
authors.initials: LJ
authors.lastname: Crofford
authors.forename: R L
authors.initials: RL
authors.lastname: Wilder
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002875:Chromosomes; D004247:DNA / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D051381:Rats / Q000235:genetics*; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D011919:Rats, Inbred Strains
chemical_list: D005819:Genetic Markers; D004247:DNA