title: Mucosal T cells recovered from mice after infection with respiratory syncytial virus display a memory/activation phenotype.
abstract: Pgp-1 expression was studied as a marker of memory/activation on systemic and mucosal T cells of BALB/c and C57BL/6 mice after infection with respiratory syncytial virus (RSV), using two-color dual fluorescence flow cytometry employing anti-L3T4 (CD4), anti-Ly2 (CD8), and anti-Pgp-1 (CD44) monoclonal antibodies. Pgp-1 was expressed in relatively low densities on T cells of C57BL/6 mice, allowing differentiation of a dual population of Pgp-1(10) and Pgp-1hi T cells after antigenic stimulation in vivo. On the contrary, T cells of BALB/c mice were uniformly Pgp-1hi, making this mouse strain less suitable for studies with this marker. In blood and spleen consistently more CD8+ than CD4+ T cells were Pgp-1hi, while in BAL more CD4+ than CD8+ T cells were Pgp-1hi. After primary but not after secondary infection, CD4+ Pgp-1hi T cells increased significantly in the blood and spleen. After secondary infection both CD4+ Pgp-1hi and CD8+ Pgp-1hi T cells increased in the BAL. It is hypothesized that after primary infection systemic RSV-specific T cells acquire an activation/memory phenotype as characterized by an enhanced expression of Pgp-1, resulting in a faster and stronger influx of these cells in the lungs after secondary infection.
authors.affiliation: Beatrix Children's Hospital, University Hospital, Groningen, The Netherlands.
authors.forename: J L
authors.initials: JL
authors.lastname: Kimpen
authors.forename: P L
authors.initials: PL
authors.lastname: Ogra
mesh_terms: D000818:Animals; D005260:Female; D018960:Hyaluronan Receptors; D007156:Immunologic Memory; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D008562:Membrane Glycoproteins / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009092:Mucous Membrane / Q000276:immunology / Q000821:virology; D010586:Phagocytes / Q000276:immunology; D012136:Respiratory Syncytial Viruses / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000821:virology
chemical_list: C476399:Cd44 protein, mouse; D018960:Hyaluronan Receptors; D008562:Membrane Glycoproteins