Analysis of recombinant human tumor necrosis factor beta by capillary electrophoresis.
Capillary electrophoresis was used for the analysis of recombinant human tumor necrosis factor beta (rhTNF-beta). Reproducible separation of the protein could be achieved in Polybrene-coated capillaries. Optimum conditions for separation were achieved with a pH 4.5 sodium acetate buffer. Besides Polybrene-coated capillaries, other commercially available column and column-coating reagents were also examined for the separation of rhTNF-beta. Capillary electrophoresis was found to be a potentially useful method for product analysis and process monitoring in recombinant-DNA technology.
Yao
Y J
YJ
Department of Chemistry, National University of Singapore.
Loh
K C
KC
Chung
M C
MC
Li
S F
SF
D004586:Electrophoresis / Q000379:methods*; D006583:Hexadimethrine Bromide / Q000737:chemistry; D006801:Humans; D008233:Lymphotoxin-alpha / Q000032:analysis*; D011993:Recombinant Fusion Proteins / Q000032:analysis
D008233:Lymphotoxin-alpha; D011993:Recombinant Fusion Proteins; D006583:Hexadimethrine Bromide