title: Association of mitogen-activated protein kinase with the microtubule cytoskeleton.
abstract: Using indirect immunofluorescence microscopy and biochemical techniques, we have determined that approximately one-third of the total mitogen-activated protein kinase (MAPK) is associated with the microtubule cytoskeleton in NIH 3T3 mouse fibroblasts. This population of enzyme can be separated from the soluble form that is found distributed throughout the cytosol and is also present in the nucleus after mitogen stimulation. The microtubule-associated enzyme pool constitutes half of all detectable MAPK activity after mitogenic stimulation. These findings extend the known in vivo associations of MAPK with microtubules to include the entire microtubule cytoskeleton of proliferating cells, and they suggest that a direct association of MAPK with microtubules may be in part responsible for the observed correlations between MAPK activities and cytoskeletal alteration.
authors.affiliation: Department of Biochemistry, University of Washington, Seattle 98195, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Reszka
authors.forename: R
authors.initials: R
authors.lastname: Seger
authors.forename: C D
authors.initials: CD
authors.lastname: Diltz
authors.forename: E G
authors.initials: EG
authors.lastname: Krebs
authors.forename: E H
authors.initials: EH
authors.lastname: Fischer
mesh_terms: D016475:3T3 Cells; D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000302:isolation & purification / Q000378:metabolism*; D002451:Cell Compartmentation; D002453:Cell Cycle / Q000502:physiology*; D002458:Cell Fractionation; D015151:Immunoblotting; D007150:Immunohistochemistry; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D017871:Calcium-Calmodulin-Dependent Protein Kinases