[Elaboration of the polymerase chain reaction system for subtype specific differentiation of influenza A virus strains].
New subtype specific primers for hemagglutinin (H1, H2, H3) of influenza A viruses were selected. The specificity of the primers' performance was tested in a model experiment which used cDNA synthesized on the vRNA from the collection of influenza A viruses (H1, H2, H3 subtypes) as templates. The sensibility of the PCR system was determined on DNA plasmids- and RNA templates (with preliminary reverse transcription) and amounted to 10(2) and 10(5)-10(6) virion particles, respectively.
Tarasov
K V
KV
Tsvirkunova
S Iu
SIu
Bekhtereva
T A
TA
D004279:DNA, Viral / Q000032:analysis; D006389:Hemagglutinins, Viral; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D013698:Templates, Genetic
D004279:DNA, Viral; D006389:Hemagglutinins, Viral; D012367:RNA, Viral
Razrabotka sistemy polimeraznoĭ tsepnoĭ reaktsii dlia subtipospetsifichnoĭ differentsiatsii shtammov virusa grippa A.