Synaptic connections and putative functions of the dopaminergic innervation of the guinea pig cochlea.
We report our findings in the guinea pig involving dopamine in postsynaptic regulation of the activity of glutamatergic inner hair cells (IHCs) and in protection of primary auditory neurons during transient ischemia. Seven days after intracochlear perfusion of 6-hydroxydopamine, no immunoreactivity to tyrosine hydroxylase (TH) was demonstrable within the organ of Corti. TH and aromatic amino acid decarboxylase were immunolocalized at an ultrastructural level within lateral olivocochlear varicosities synapsing with radial auditory dendrites postsynaptic to the IHCs. The D2 agonist piribedil induced a dose-dependent decrease in the amplitude of the compound action potential of the auditory nerve. Piribedil also prevented appearance of ischemia-induced swelling of the radial dendrites.
d'Aldin
C
C
INSERM U-254, Montpellier, France.
Eybalin
M
M
Puel
J L
JL
Charachon
G
G
Ladrech
S
S
Renard
N
N
Pujol
R
R
D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy; D003051:Cochlea / Q000166:cytology / Q000187:drug effects / Q000294:innervation*; D018491:Dopamine Agonists / Q000494:pharmacology*; D007758:Ear, Inner / Q000166:cytology / Q000187:drug effects; D006168:Guinea Pigs / Q000502:physiology*; D006198:Hair Cells, Auditory / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D006801:Humans; D009925:Organ of Corti / Q000187:drug effects / Q000502:physiology; D016627:Oxidopamine; D010891:Piribedil / Q000494:pharmacology / Q000627:therapeutic use; D017729:Presynaptic Terminals / Q000187:drug effects / Q000502:physiology*; D017448:Receptors, Dopamine D2 / Q000378:metabolism / Q000502:physiology*
D018491:Dopamine Agonists; D017448:Receptors, Dopamine D2; D016627:Oxidopamine; D010891:Piribedil