Role of the kidney in the production of a low molecular weight growth factor (MW < 1000 Da): experimental study in the pig.
Small peptide molecules known as low molecular weight growth factor (LMW-GF) have been identified in human serum. They enhance the effect of IGF-1 (insulin-like growth factor) on proteoglycan synthesis. In the present work we investigated the role played by the kidney in the production of LMW-GF, using the pig as an experimental model. Six pigs underwent bilateral nephrectomy followed 24 h later by orthotopic autotransplantation of the kidney. Renal and liver functions were evaluated by measurement of serum creatinine, urea, electrolytes, amino transferases (ASAT, ALAT), proteins, and bilirubin. LMW-GF was measured by bioassay using 11-day-old pelvic chick embryo cartilages. We observed that LMW-GF quickly disappeared from pig serum after nephrectomy and only reappeared when transplantation was successful. Reappearance of LMW-GF can precede improvement of renal function evaluated by plasma creatinine levels. These data appear to demonstrate that the kidney is involved in LMW-GF production.
Jacob
C
C
Laboratory of Medical Biochemistry I and IBN School of Medicine, Vandoeuvre, France.
Hubert
J
J
Maachi
F
F
Punga-Maole
A
A
Dousset
B
B
Junke
E
E
Belleville
F
F
D058186:Acute Kidney Injury / Q000378:metabolism* / Q000503:physiopathology; D000818:Animals; D001681:Biological Assay; D002642:Chick Embryo; D006133:Growth Substances / Q000096:biosynthesis* / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000502:physiology; D007668:Kidney / Q000378:metabolism* / Q000503:physiopathology; D016030:Kidney Transplantation / Q000502:physiology; D008099:Liver / Q000378:metabolism / Q000503:physiopathology; D009392:Nephrectomy; D013552:Swine; D014182:Transplantation, Autologous
D006133:Growth Substances; D007334:Insulin-Like Growth Factor I