title: Herbicide-induced DNA damage in human lymphocytes evaluated by the single-cell gel electrophoresis (SCGE) assay.
abstract: The genotoxicity of the herbicides, alachlor, atrazine, maleic hydrazide, paraquat and trifluralin has been evaluated in the single-cell gel electrophoresis (SCGE) assay by using human peripheral blood lymphocytes. All treatments were conducted with and without the presence of an external bioactivation source (S9 mix). The results indicate that all the herbicides tested are able to give positive results by increasing the comet tail length, which would confirm both the genotoxicity of the herbicides and the sensitivity of the assay in front of these chemicals. Alachlor and atrazine give similar results in treatments with and without S9, while when the S9 mix was not used paraquat and trifluralin genotoxicity was higher. On the other hand, although maleic hydrazide genotoxicity was higher when S9 mix was used at normal pH (7.4), our data show that its genotoxicity depends largely on the pH solution, increasing as the pH decreased.
authors.affiliation: Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: G
authors.initials: G
authors.lastname: Ribas
authors.forename: G
authors.initials: G
authors.lastname: Frenzilli
authors.forename: R
authors.initials: R
authors.lastname: Barale
authors.forename: R
authors.initials: R
authors.lastname: Marcos
mesh_terms: D001711:Biotransformation; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D004586:Electrophoresis / Q000379:methods*; D006540:Herbicides / Q000493:pharmacokinetics / Q000633:toxicity*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D009153:Mutagens / Q000493:pharmacokinetics / Q000633:toxicity*
chemical_list: D006540:Herbicides; D009153:Mutagens; D004247:DNA