title: Characterization of a delta opioid receptor in rat pheochromocytoma cells.
abstract: In one subclone of PC12 pheochromocytoma cells, PC12h, the levels of delta opioid receptors markedly increase in response to nerve growth factor (NGF). This increase, as assessed by [3H]diprenorphine binding, is found only under specific culture conditions. NGF treatment of PC12h cells also results in the induction of delta opioid receptor (DOR-1) mRNA. The time course for NGF induction of mRNA and protein is similar, although the levels of mRNA increase approximately 5-fold, whereas the levels of receptor increase only 2-fold. Competition studies with selective delta ([D-Pen2,5]-enkephalin, DPDPE), mu ([D-Ala2,N-Me-Phe4,Gly-ol5]- enkephalin, DAMGO) and kappa (trans-[+/-]-3,4-dichloro-N-methyl-N-[2-(1-pyrrolidinyl)-cyclohexyl]- benzenacetamide methansulfonate salt, U50,488) opioid agonists to displace [3H]diprenorphine confirm that the delta subtype of opioid receptor is present on PC12h cells. The delta opioid receptor is coupled functionally, as indicated by agonist inhibition of forskolin-stimulated cAMP accumulation in a naloxone-reversible manner. Finally, the delta opioid receptor was cloned from PC12h cells. The sequence was identical with that described previously for the rat clone. The PC12h cell line thus provides a model system in which to study regulation of delta opioid receptors.
authors.affiliation: Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Abood
authors.forename: Q
authors.initials: Q
authors.lastname: Tao
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000494:pharmacology; D016716:PC12 Cells; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017465:Receptors, Opioid, delta / Q000819:agonists / Q000235:genetics / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D017465:Receptors, Opioid, delta; D000262:Adenylyl Cyclases