title: A cocaine-sensitive active dopamine transport in human lymphocytes.
abstract: Human lymphocytes possess a cocaine-sensitive high-affinity transport system for [3H]dopamine. [3H]Dopamine uptake was saturated with increasing dopamine concentrations and followed Michaelis-Menten kinetics. The uptake was temperature, sodium, and chloride dependent and was affected by the co-addition of ouabain, phloridzin, potassium cyanide, gramicidin, and other metabolic inhibitors. The uptake of dopamine was blocked significantly in a concentration-dependent manner by cocaine and its congeners. Furthermore, preliminary evidence is presented linking the possible relationship between decreased lymphocyte [3H]dopamine uptake and chronic cocaine abuse in humans.
authors.affiliation: Department of Radiology (Division of Nuclear Medicine), Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Faraj
authors.forename: Z L
authors.initials: ZL
authors.lastname: Olkowski
authors.forename: R T
authors.initials: RT
authors.lastname: Jackson
mesh_terms: D000328:Adult; D001692:Biological Transport / Q000187:drug effects; D003042:Cocaine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004298:Dopamine / Q000378:metabolism*; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009293:Opioid-Related Disorders / Q000378:metabolism; D013329:Structure-Activity Relationship; D019966:Substance-Related Disorders / Q000378:metabolism
chemical_list: D018765:Dopamine Uptake Inhibitors; D003042:Cocaine; D004298:Dopamine