Overexpression of p53 and MDM2 proteins in cervical neoplasia.
Sixteen methacarn-fixed and paraffin-embedded cervical squamous lesions were examined using monoclonal antibodies to p53 and MDM2 in order to compare the expression of both proteins in cervical neoplasia. Standard avidin-biotin or streptavidin-biotin immunoperoxidase techniques were employed. The results show that the overexpression of both proteins takes place in a meaningful proportion of cervical neoplastic lesions. The expression of either protein is not very frequent in CIN 1 and 2. The overexpression of either p53 (9/12) or MDM2 (4/10) proteins was recorded in the group of more advanced lesions, their level fluctuating from 10% to 60% positive cells. The results suggest the possibility of an interaction of the p53 and MDM2 proteins in some cases of cervical neoplasia.
Lukás
Z
Z
2nd Department of Pathology, J. G. Mendel Children University Hospital, Brno, Czech Republic.
Vojtĕsek
B
B
Anton
M
M
Picksley
S M
SM
Kubálek
V
V
D000085:Acetates; D019342:Acetic Acid; D000911:Antibodies, Monoclonal; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D002725:Chloroform; D005260:Female; D005404:Fixatives; D015870:Gene Expression; D006652:Histological Techniques; D006801:Humans; D000432:Methanol; D009363:Neoplasm Proteins / Q000032:analysis / Q000096:biosynthesis; D009687:Nuclear Proteins; D010232:Paraffin; D011518:Proto-Oncogene Proteins / Q000032:analysis / Q000096:biosynthesis*; D051736:Proto-Oncogene Proteins c-mdm2; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology*
D000085:Acetates; D000911:Antibodies, Monoclonal; D005404:Fixatives; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D016159:Tumor Suppressor Protein p53; C046544:methacarn; D002725:Chloroform; D010232:Paraffin; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2; D019342:Acetic Acid; D000432:Methanol