A Klebsiella aerogenes moaEF operon is controlled by the positive MoaR regulator of the monoamine regulon.
A 30-kDa protein accumulated upon induction by a high concentration of tyramine or dopamine in cells of Klebsiella aerogenes (Ka). These cells carried a plasmid (pAS123) that included the arylsulfatase operon (atsBA). Deletion analysis showed that the region essential for induction of the 30-kDa protein was located within a 2.0-kb cloned segment downstream of the atsBA operon. The nucleotide (nt) sequence of the 2.0-kb fragment revealed two open reading frames (ORFs), moaE and moaF. Transcription from a putative promoter of moaE was induced by the addition of tyramine, and the moaF gene was co-transcribed from this monoamine-inducible Ka promoter. The deduced Ka MoaE protein was homologous to insect-type alcohol dehydrogenase. The sequence of the 18 amino acids from the N-terminus of the purified 30-kDa protein agreed with that deduced from the nt sequence of moaF. Using a Ka strain with a mutant moaR gene, we found that MoaR, that acts as the positive regulator of the monoamine regulon, also acts as the positive regulator of the moaEF operon.
Azakami
H
H
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
Sugino
H
H
Iwata
N
N
Yokoro
N
N
Yamashita
M
M
Murooka
Y
Y
D000426:Alcohol Dehydrogenase / Q000235:genetics; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D015306:Biogenic Monoamines / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D018085:Regulon; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics; D014157:Transcription Factors; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D015306:Biogenic Monoamines; D004268:DNA-Binding Proteins; C096552:MoaF protein, Klebsiella aerogenes; C083495:MoaR protein, Klebsiella aerogenes; D014157:Transcription Factors; D013401:Sugar Alcohol Dehydrogenases; D000426:Alcohol Dehydrogenase; C096551:MoaE protein, Klebsiella aerogenes; C022529:ribitol 2-dehydrogenase