Regulation of histamine release from human bronchoalveolar lavage mast cells by stem cell factor in several respiratory diseases.
We investigated the effects of stem cell factor (SCF) on histamine release (HR) from human bronchoalveolar lavage (BAL) mast cells. BAL cells were recovered from lavage performed in patients undergoing clinical bronchoscopy. SCF (0.02-20 ng/ml), which is by itself a poor secretagogue (mean +/- SEM HR: 3.7 +/- 0.9%; n = 27), strongly enhanced HR induced by anti-IgE in a concentration-related manner. Significant potentiation began at 0.2 ng/ml (30 +/- 10%; p < 0.05; n = 12) and reached a plateau at 2 ng/ml (40 +/- 10%; P < 0.01 at 2 ng/ml and 45 +/- 10%; P < 0.01 at 20 ng/ml; n = 12). In contrast, SCF failed to enhance HR induced by calcium ionophore A23187. Among the BAL cell samples initially unresponsive to anti-IgE (55% of samples), 36% (10/28) were converted to responders if the cells were shortly preincubated with SCF. In 25% of samples (7/27), SCF (20 ng/ml) caused direct HR of 10 +/- 2.1%. The mast cells which released histamine when challenged with SCF also secreted higher levels of histamine in response to anti-IgE and calcium ionophore than those nonresponsive to SCF. While interleukin (IL)-3 and IL-5 (20 ng/ml) were unable to modulate immunologic HR, GM-CSF (20 ng/ml) produced significant potentiation (P < 0.05), which was, however, smaller than that observed with SCF.(ABSTRACT TRUNCATED AT 250 WORDS)
Louis
R
R
Department of Pneumology, University Hospital, Sart-Tilman, Liège, Belgium.
Tilkin
P
P
Poncelet
M
M
Corhay
J L
JL
Mendez
P
P
Weber
T
T
Radermecker
M
M
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000888:Antibodies, Anti-Idiotypic / Q000494:pharmacology; D001980:Bronchi / Q000187:drug effects / Q000276:immunology; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology*; D000001:Calcimycin / Q000494:pharmacology; D016022:Case-Control Studies; D002452:Cell Count; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000494:pharmacology; D008171:Lung Diseases / Q000276:immunology*; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D008875:Middle Aged; D019089:Stem Cell Factor / Q000494:pharmacology*; D007507:Therapeutic Irrigation / Q000379:methods; D013997:Time Factors
D000276:Adjuvants, Immunologic; D000888:Antibodies, Anti-Idiotypic; D016207:Cytokines; D019089:Stem Cell Factor; D007073:Immunoglobulin E; D000001:Calcimycin