Studies on T-cell receptors involved in experimental autoimmune encephalomyelitis using the complementary peptide recognition approach.
Based upon Blalock's complementary recognition approach, a complementary or antisense peptide (CP) was designed to the experimental autoimmune encephalomyelitis (EAE) epitope peptide, rat myelin basic protein (MBP) peptide 72-82. This peptide (EAE CP) was shown to have some sequence similarities to T-cell receptors (TCR) and MHC II molecules in a sequence homology search. Solid-phase binding assays demonstrated specific and high affinity binding (3 and 4 microM) between the EAE CP and the rat and guinea pig EAE epitope peptides (Rt72-82 and Gp69-82), respectively. This EAE CP was also found to be immunogenic in rats in an ear swelling test for delayed type hypersensitivity (DTH) reactions and an ELISA for antibody responses. However, a rabbit antibody generated to EAE CP was shown to be unable to stain the V beta 8+ EAE susceptible T-cells in immunofluorescence analyses. This EAE CP was also used in attempts to down-regulate EAE and the results showed that prior immunization with EAE CP in complete Freund's adjuvant could not prevent the Lewis rats from developing EAE. Although the data on sense-antisense peptide interaction were positive and the EAE CP was immunogenic, the inability of EAE CP to regulate EAE indicates that the CP approach may not be generally applicable.
Xian
C J
CJ
Biotechnology Program, School of Biological and Environmental Sciences, Murdoch University, Western Australia.
Simmons
R D
RD
Willenborg
D O
DO
Vandenbark
A A
AA
Hashim
G A
GA
Carnegie
P R
PR
D000595:Amino Acid Sequence; D000818:Animals; D016375:Antisense Elements (Genetics) / Q000276:immunology / Q000378:metabolism; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000503:physiopathology* / Q000517:prevention & control; D000939:Epitopes; D005821:Genetic Techniques; D006168:Guinea Pigs; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D007074:Immunoglobulin G / Q000276:immunology; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology
D016375:Antisense Elements (Genetics); D018076:DNA, Complementary; D000939:Epitopes; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G; D004676:Myelin Basic Protein; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell