Neural cell adhesion molecule (N-CAM) distribution may predict the effect of neurotoxins on the brain.
The neural cell adhesion molecule (N-CAM) is a convenient neurospecific marker for investigating the effects of neurotoxins on cell migration, cell recognition and differentiation of neurons during development. In this report, we discuss the developmental toxicity of valproic acid studied by two different approaches (the immunochemical detection of N-CAM content and polypeptide composition, and immunohistochemical analysis of N-CAM topography). Immunohistochemical analysis of distribution of N-CAM as a surface marker on the neural cells predicted the effect of the neurotoxin.
Ushakova
G A
GA
Department of Biophysics and Biochemistry, State University, Dniepropetrovsk, Ukraine.
Berezin
V A
VA
Skibo
G G
GG
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology / Q000378:metabolism; D015152:Blotting, Northern; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D001923:Brain Chemistry / Q000187:drug effects*; D007150:Immunohistochemistry; D008566:Membranes / Q000187:drug effects / Q000648:ultrastructure; D008854:Microscopy, Electron; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D009498:Neurotoxins / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D014635:Valproic Acid / Q000494:pharmacology
D000954:Antigens, Surface; D019006:Neural Cell Adhesion Molecules; D009498:Neurotoxins; D014635:Valproic Acid