Which nutrient supplements for optimal HIV-1 production by cultured human lymphocytes?
Under standard conditions, all established cell-lines of HIV-1-infected lymphocytes are cultured in a medium containing a high percentage of serum. We have found that whilst partial or total serum depletion impedes their cell growth, it dramatically increases their virus production. Serial subcultures in serum-depleted medium are necessary to insure permanent cell growth and a relatively high reverse transcriptase activity. Following optimization of culture conditions, both HIV-infected and uninfected H9 cells have been regularly grown in a new protein-free medium. Such chemically defined culture conditions were required to detect the effect of minor components, such as vitamins, on host cell-virus interactions.
Landureau
J C
JC
Laboratoire de Physiologie Cellulaire, Université Pierre et Marie Curie, Paris, France.
Achour
A
A
Fouchard
M
M
M'Bika
J P
JP
Mazière
J C
JC
Zagury
D
D
D001798:Blood Proteins; D002455:Cell Division; D002460:Cell Line; D002784:Cholesterol / Q000494:pharmacology; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004279:DNA, Viral / Q000096:biosynthesis; D054303:HIV Reverse Transcriptase; D015497:HIV-1 / Q000254:growth & development*; D006801:Humans; D015999:Multivariate Analysis; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012256:Riboflavin / Q000494:pharmacology; D013997:Time Factors; D014779:Virus Replication / Q000187:drug effects
D001798:Blood Proteins; D003470:Culture Media; D016895:Culture Media, Serum-Free; D004279:DNA, Viral; D002784:Cholesterol; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase; D012256:Riboflavin