Aminoacid recovery via microdialysis and photoinduced focal cerebral ischemia in brain cortex of rats.
A photochemical method using the Rose Bengal dye as thrombogenic agent was employed to induce focal cerebral ischemia in frontoparietal cortex of rats. A transcerebral microdialysis probe was used to collect samples from ischemic cortical area. An increase in glutamate (6-fold) and in taurine (4-fold) within the first hour occurred. Neuropathological investigations demonstrate a reproducible damaged area surrounded by a thin peripheral area showing neuronal apoptotic phenomena. The method represents a reproducible model of focal cerebral ischemia with neuropathological aspects superimposable to those characteristic of thrombogenic stroke in man. This method could also be relevant in the study of neurotransmitters during the evolution of ischemia. Furthermore, the presence of apoptotic phenomena in the perilesional halo confirms an ischemic penumbra suggesting the significance of preclinical pharmacological trials.
Montalbetti
L
L
Istituto Neurologico C. Mondino, IRCCS Università di Pavia, Italy.
Rozza
A
A
Rizzo
V
V
Favalli
L
L
Scavini
C
C
Lanza
E
E
Savoldi
F
F
Racagni
G
G
Scelsi
R
R
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D002545:Brain Ischemia / Q000139:chemically induced*; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism*; D004195:Disease Models, Animal; D018698:Glutamic Acid / Q000378:metabolism; D008297:Male; D017551:Microdialysis; D018377:Neurotransmitter Agents / Q000378:metabolism; D010777:Photochemistry; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012395:Rose Bengal; D013654:Taurine / Q000378:metabolism
D000596:Amino Acids; D018377:Neurotransmitter Agents; D013654:Taurine; D012395:Rose Bengal; D018698:Glutamic Acid