Cloning and characterization of Wnt-4 and Wnt-11 cDNAs from chick embryo.
We have isolated two members of the Wnt gene family, Wnt-4 and Wnt-11, from chick embryo cDNA library, and determined the entire coding sequences. The Wnt-4 and Wnt-11 genes encode secretory proteins composed of 351 and 354 amino acids, respectively, both having 24 Cys residues conserved among other Wnt family members. Alignment of the deduced amino acid sequences reveals that chicken Wnt-4 and Wnt-11 are most similar to Xenopus Wnt-4 and mouse Wnt-11; respectively. Northern blot analysis indicates the Wnt-4 expression at 1.5 kilobase and the Wnt-11 expression at 2.0 kilobase in the chick embryo.
Tanda
N
N
Department of Pharmacology, Kawasaki Medical School, Okayama, Japan.
Kawakami
Y
Y
Saito
T
T
Noji
S
S
Nohno
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002642:Chick Embryo / Q000502:physiology*; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D015723:Gene Library; D006023:Glycoproteins; D006031:Glycosylation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals; D011506:Proteins / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D051153:Wnt Proteins; D060528:Wnt4 Protein; D029867:Xenopus Proteins
D017931:DNA Primers; D018076:DNA, Complementary; D006023:Glycoproteins; D021382:Protein Sorting Signals; D011506:Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D051153:Wnt Proteins; D060528:Wnt4 Protein; C555584:Wnt4 protein, Xenopus; C555582:Wnt4 protein, mouse; D029867:Xenopus Proteins; C512785:wnt11b protein, Xenopus