Ribozyme mediated cleavage of acute phase serum amyloid A (A-SAA) mRNA in vitro.
The 1000-fold induction of acute phase serum amyloid A (A-SAA) in the liver during inflammation indicates that this protein plays an important, though ill-defined, role in host defence. Paradoxically, prolonged overproduction of A-SAA is a causative factor in secondary amyloidosis and possibly other diseases such as atherosclerosis; the ability to down-regulate A-SAA synthesis is therefore of considerable clinical importance. We have successfully generated anti-SAA hammerhead ribozymes and we report that they are capable of cleaving A-SAA mRNA in vitro.
Gaughan
D J
DJ
Department of Genetics and Biotechnology Institute, Trinity College, University of Dublin, Ireland.
Steel
D M
DM
Whitehead
A S
AS
D000209:Acute-Phase Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D017931:DNA Primers; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D000685:Serum Amyloid A Protein / Q000235:genetics*; D013379:Substrate Specificity
D000209:Acute-Phase Proteins; D017931:DNA Primers; D016337:RNA, Catalytic; D012333:RNA, Messenger; D000685:Serum Amyloid A Protein