title: Alteration in the fluorescence polarization of rat plasma and liver cell membranes following bile duct ligation in rats.
abstract: The fluorescence polarization levels of liver cell membranes and plasma were analyzed to determine membrane fluidity following bile duct ligation (BDL) in rats. Fluorescence polarization was measured with a spectrofluorophotometer equipped with polarizers, using 1,6-diphenyl-1,3,5-hexatrien (DPH) as a probe. After bile duct ligation, liver cell membrane fluidity decreased significantly for up to 14 days after surgery (P < 0.001 on 3rd and 7th days). The polarization of the plasma in rats with BDL slightly but significantly increased compared to the levels in the control animals over the 14-day period following BDL. In addition, a small but significant correlation in the polarization levels between plasma and liver cell membranes (r = 0.362, P < 0.02) was observed. The co-incubation of BDL plasma with normal liver cell membranes resulted in a decrease in membrane fluidity, which suggested that BDL rat plasma had a direct effect on membrane fluidity. After a 70% hepatectomy, the polarization of the membranes from remnant livers in the BDL rats remained elevated relative to the sham-operated controls. It is thus concluded that the membrane fluidity of the livers in BDL rats decreases following bile duct ligation and does not increase after a 70% hepatectomy, presumably due to the increased plasma level of bilirubin.
authors.affiliation: First Department of Surgery, Kyoto University School of Medicine, Japan.
authors.forename: W B
authors.initials: WB
authors.lastname: Zhong
authors.forename: J
authors.initials: J
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Komoto
authors.forename: T
authors.initials: T
authors.lastname: Kasamatsu
authors.forename: M
authors.initials: M
authors.lastname: Yoshida
authors.forename: K
authors.initials: K
authors.lastname: Fujita
authors.forename: T
authors.initials: T
authors.lastname: Kaido
authors.forename: N
authors.initials: N
authors.lastname: Funaki
authors.forename: M
authors.initials: M
authors.lastname: Imamura
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000097:blood; D002462:Cell Membrane / Q000502:physiology*; D001651:Cholestasis, Extrahepatic / Q000503:physiopathology*; D005454:Fluorescence Polarization; D006498:Hepatectomy; D008099:Liver / Q000503:physiopathology*; D008115:Liver Regeneration / Q000502:physiology; D008560:Membrane Fluidity / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001663:Bilirubin