Detection of Bartonella (Rochalimaea) henselae bacteremia using BacT/Alert blood culture system.
Bartonella henselae was isolated from the blood of five febrile immunosuppressed patients using BacT/Alert (Organon Teknika, Durham, NC) automated microbial detection system. An immunofluorescence assay (using 1:1000 dilutions) was used to confirm identification of fastidious, pleomorphic, non-Gram staining, argyrophilic bacilli displaying rachety motility that had been presumptively identified as Bartonella spp. The practicality of identification of Bartonella henselae using goat antisera for use in a routine clinical microbiology laboratory was demonstrated by this study.
Tierno
P M
PM
Department of Microbiology, New York University Medical Center, New York, NY 10016, USA.
Inglima
K
K
Parisi
M T
MT
D017088:AIDS-Related Opportunistic Infections / Q000150:complications; D000328:Adult; D016917:Angiomatosis, Bacillary / Q000175:diagnosis*; D016470:Bacteremia / Q000382:microbiology*; D001431:Bacteriological Techniques / Q000295:instrumentation*; D001474:Bartonella Infections / Q000175:diagnosis / Q000382:microbiology; D018416:Bartonella henselae / Q000302:isolation & purification*; D004396:Coloring Agents; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D013997:Time Factors
D004396:Coloring Agents