Virion positions and relationships of lactococcal temperate bacteriophage TP901-1 proteins.
The major proteins of phage TP901-1 virion were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and structural relations were determined using specific antibodies, obtained by affinity purification from a polyclonal serum. A 23-kDa protein was identified as the major tail protein, and a 31-kDa molecule as the major head protein, respectively. Labeling experiments with antibodies against two proteins, with molecular masses of 20 and 19 kDa, indicated that they were baseplate-related components. A 72-kDa protein was found to be part of a neck passage structure, which includes a collar. Evidence for the presence of attached whiskers was also obtained. T7 RNA polymerase-mediated expression of the two major proteins confirmed the gene location of the previously sequenced region of the phage genome. The relation to other lactococcal phages was determined by DNA hybridization and antibody probing, showing that despite low DNA similarity, TP901-1 NPS epitopes were detected in both related and unrelated small isometric-headed phages.
Johnsen
M G
MG
Danish Center for Lactic Acid Bacteria, Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg C.
Neve
H
H
Vogensen
F K
FK
Hammer
K
K
D000914:Antibodies, Viral; D000918:Antibody Specificity; D001435:Bacteriophages / Q000737:chemistry* / Q000276:immunology / Q000648:ultrastructure; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics; D013294:Lactococcus lactis / Q000821:virology; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D015678:Viral Structural Proteins / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D014771:Virion / Q000737:chemistry* / Q000648:ultrastructure
D000914:Antibodies, Viral; D004279:DNA, Viral; D011993:Recombinant Fusion Proteins; D015678:Viral Structural Proteins