title: Identification of an endotoxin and IFN-inducible cDNA: possible identification of a novel protein family.
abstract: The response of macrophages to agents such as lipopolysaccharide (LPS) and interferon (IFN) includes the transcriptional activation of numerous genes. We have used the method of differential screening of a RAW 264.7 macrophage cell line cDNA library to isolate and characterize LPS-induced messages. One such message, LRG-47, is induced by LPS, IFN-gamma, and IFN-alpha/beta, but not by a panel of other cytokines or pharmacological activating agents. LRG-47 is homologous to two other IFN-gamma-induced genes, IRG-47 and Mg21. The LRG-47 sequence is approximately 33% identical and 52% similar to both these putative protein products. All three putative proteins, particularly Mg21, bear homology to a T cell product, Tgtp, induced by T cell receptor cross-linking. The three macrophage-derived proteins share areas of homology with GTP-binding proteins, are approximately 415 amino acids in length, and have similar kinetics of induction by IFN-gamma. This suggests that these genes may be members of a new family of IFN-inducible proteins.
authors.affiliation: Department of Pathology, Baltimore VA Medical Center, Maryland 21201, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Sorace
authors.forename: R J
authors.initials: RJ
authors.lastname: Johnson
authors.forename: D L
authors.initials: DL
authors.lastname: Howard
authors.forename: B E
authors.initials: BE
authors.lastname: Drysdale
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism; D001483:Base Sequence; D002478:Cells, Cultured; D002999:Clone Cells; D018076:DNA, Complementary / Q000032:analysis* / Q000096:biosynthesis* / Q000235:genetics; D019204:GTP-Binding Proteins / Q000737:chemistry; D007372:Interferons / Q000494:pharmacology*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D013268:Stimulation, Chemical
chemical_list: D018076:DNA, Complementary; C096000:Ifi1 protein, mouse; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007372:Interferons; D019204:GTP-Binding Proteins