Isolation of a human allo-peptide presented by HLA-B51 molecules.
Recent studies have demonstrated directly that alloreactive mouse CTL recognize peptides presented by MHC class I molecules. However, there is no direct evidence that human alloreactive CTL recognize peptides presented by HLA class I molecules. We have isolated an HLA-B51 alloreactive CTL clone, 2B3, that did not kill the TAP defective cell lines T2 and .174, whereas it killed the TAP-positive cell line T1 and .174 cells transfected with TAP genes. These findings suggested that this clone recognizes a TAP-dependent allo-peptide. We attempted to isolate the human allo-peptide recognized by the 2B3 clone from HLA-B51 molecules. A naturally occurring HLA-B*5101 binding peptide isolated from T1 cells was recognized by the 2B3 clone. The peptide was also isolated from HLA-B*5101 molecules purified from C1R-B*5101 cells. In the present study, we directly demonstrated that a human alloreactive CTL clone recognizes peptide presented by HLA class I molecules.
Tomiyama
H
H
Department of Tumor Biology, University of Tokyo, Japan.
Takamiya
Y
Y
Hill
A B
AB
Cerundolo
V
V
Kelly
A
A
Egawa
K
K
Trowsdale
J
J
Takiguchi
M
M
D017951:Antigen Presentation; D002460:Cell Line; D015235:HLA-B Antigens / Q000276:immunology*; D059949:HLA-B51 Antigen; D006801:Humans; D007519:Isoantigens / Q000737:chemistry / Q000302:isolation & purification*; D010455:Peptides / Q000276:immunology* / Q000302:isolation & purification; D013602:T-Lymphocytes, Cytotoxic / Q000737:chemistry
D015235:HLA-B Antigens; D059949:HLA-B51 Antigen; D007519:Isoantigens; D010455:Peptides