Fed before diethylnitrosamine, Fusarium moniliforme and F proliferatum mycotoxins alter the persistence of placental glutathione S-transferase-positive hepatocytes in rats.
Groups of 5-w-old F344/N female rats were fed a semipurified diet for 13 w with or without 20 mg fumonisin B1/kg provided from an aqueous extract of Fusarium moniliforme-corn culture. After 1 w, a single dose of 30 mg diethylnitrosamine (DEN)/kg was given orally. Twelve weeks later, the presence of placental glutathione S-transferase-positive (PGST-[+]) hepatocytes were immunohistochemically quantified. Rats given DEN and the FB1-containing diet for 1 or 13 w developed 4-fold more PGST-[+] hepatocytes than rats given DEN alone. In a second study, male and female F344/N rats were fed 20 mg purified FB1/kg diet or F proliferatum-corn culture material containing 20 mg FB1/kg diet for 1 w before DEN treatment. One week after DEN treatment, male rats fed the F proliferatum-corn culture material had significantly fewer PGST-[+] hepatocytes than those fed DEN with or without purified FB1. At 9 w after DEN treatment, PGST-[+] cells in female rats given DEN and fed F proliferatum-corn culture material were more persistent than in rats given DEN alone. Males given DEN and fed FB1 or F proliferatum culture material had significantly fewer PGST-[+] hepatocytes than males given DEN alone. These results suggest that F moniliforme and F proliferatum components are cocarcinogens in females. In males, however, FB1 and unidentified F proliferatum components reduced the persistence of DEN-initiated preneoplastic hepatocytes.
Lebepe-Mazur
S
S
Iowa State University, Ames 50011, USA.
Hopmans
E
E
Murphy
P A
PA
Hendrich
S
S
D000284:Administration, Oral; D000704:Analysis of Variance; D000818:Animals; D002273:Carcinogens / Q000633:toxicity*; D002274:Carcinogens, Environmental / Q000633:toxicity; D003470:Culture Media; D004032:Diet; D004052:Diethylnitrosamine / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D037341:Fumonisins; D005670:Fusarium / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism*; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D009183:Mycotoxins / Q000633:toxicity*; D010920:Placenta / Q000201:enzymology; D051381:Rats; D011916:Rats, Inbred F344
D002273:Carcinogens; D002274:Carcinogens, Environmental; D003470:Culture Media; D037341:Fumonisins; D009183:Mycotoxins; D004052:Diethylnitrosamine; C056933:fumonisin B1; D005982:Glutathione Transferase