title: Degradation of sinigrin by Lactobacillus agilis strain R16.
abstract: Forty-two lactobacilli were screened for their potential to degrade glucosinolate sinigrin. One of them, strain R16, demonstrated a high level of sinigrin degradation; it was identified as Lactobacillus agilis. The sinigrin degrading activity of L. agilis R16 could only be demonstrated when intact cells were used. The products of sinigrin degradation are allyl-isothiocyanate (AITC) and glucose (which is further fermented to DL-lactic acid), suggesting that myrosinase activity is involved. The activity was induced by the presence of sinigrin. Glucose inhibited the myrosinase activity, even in induced cells. Lactobacillus agilis R16 was able to grow on an extract of brown mustard seed and caused glucosinolate degradation.
authors.affiliation: Departamento de Química Analítica y Tecnología de Alimentos, Facultad de Ciencias Químicas, Universidad de Castilla-La Mancha, Ciudad Real, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Llanos Palop
authors.forename: J P
authors.initials: JP
authors.lastname: Smiths
authors.forename: B T
authors.initials: BT
authors.lastname: Brink
mesh_terms: D001673:Biodegradation, Environmental; D005961:Glucosinolates / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000502:physiology; D017879:Isothiocyanates / Q000378:metabolism; D007778:Lactobacillus / Q000254:growth & development / Q000378:metabolism*; D009149:Mustard Plant; D010936:Plant Extracts / Q000378:metabolism; D010946:Plants, Medicinal; D013045:Species Specificity
chemical_list: D005961:Glucosinolates; D017879:Isothiocyanates; D010936:Plant Extracts; C010330:sinigrin; C004471:allyl isothiocyanate; D006026:Glycoside Hydrolases; C022739:thioglucosidase