Optimization of the Magnetic Bead Antigen Capture Enzyme Immuno Assay for the detection of circulating anodic antigens in mixed Schistosoma infections.
In the present study, simplification and adaptation of the Magnetic Bead Antigen Capture Enzyme Immuno Assay (MBAC-EIA) technique for detection of circulating anodic antigens (CAA) under field conditions was achieved. It was shown that the assay could be performed successfully within the broad temperature range of 18-37 degrees C. The slightly lower sensitivity observed at low temperatures could be adjusted for by prolonging the incubation period. Shaking the plate by hand was as good as automatic mechanical shaking, aspiration of the supernatant before the addition of conjugate was not necessary, and the use of whole blood and serum offered similar assay sensitivity. Furthermore incubation times could be considerably shortened without loss of sensitivity. A major advantage of the MBAC-EIA was that the beads, after elution of bound components, were found to be reusable. The study also showed that the sensitivity of the MBAC-EIA technique in diagnosis of schistosomiasis in a Zimbabwean community endemic for both urinary and intestinal schistosomiasis, was 94%.
Ndhlovu
P
P
Blair Research Laboratory, Harare, Zimbabwe.
Cadman
H
H
Gundersen
S G
SG
Vennervald
B
B
Friis
H
H
Christensen
N
N
Mutasa
G
G
Haagensen
I
I
Chandiwana
S K
SK
Deelder
A M
AM
D000293:Adolescent; D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D002648:Child; D006801:Humans; D007124:Immunoenzyme Techniques; D008280:Magnetics; D012548:Schistosoma haematobium / Q000276:immunology*; D012550:Schistosoma mansoni / Q000276:immunology*; D012552:Schistosomiasis / Q000175:diagnosis*; D013696:Temperature; D013997:Time Factors
D000947:Antigens, Helminth