Intestinal amebiasis: cyclic suppression of the immune response.
The cellular immune response was evaluated in a C3H/HeJ mouse model of intestinal amebiasis at 5-60 days postinoculation with Entamoeba histolytica. At various intervals, spleen lymphocytes were obtained from infected and noninfected control mice and cultured with concanavalin A (Con A), pokeweed mitogen (PWM), or ameba antigen. E. histolytica infection induced a cyclic depression of DNA synthesis when spleen lymphocytes were stimulated with a T-cell mitogen (Con A), a T- and B-cell (PWM) mitogen, or an ameba antigen. A similar response was observed in the determinations of interleukin-2 in the supernatants of Con A-stimulated spleen cells from infected mice. When spleen cells from E. histolytica-infected mice were stimulated with phorbol myristate acetate plus ionomycin, results indicated a signal-transduction defect. These alterations, observed at the cellular level, might facilitate invasion of the host by the parasite.
Ghosh
P K
PK
Departamento de Inmunología, Universidad Nacional Autónoma de México, México, D. F.
Castellanos-Barba
C
C
Ortiz-Ortiz
L
L
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D004195:Disease Models, Animal; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000276:immunology*; D007111:Immunity, Cellular; D007376:Interleukin-2 / Q000032:analysis; D007411:Intestinal Diseases, Parasitic / Q000276:immunology*; D015759:Ionomycin / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D008934:Mitogens; D015398:Signal Transduction; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000953:Antigens, Protozoan; D007376:Interleukin-2; D008934:Mitogens; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate