title: Crystallization and preliminary X-ray crystallographic studies on the adenovirus ssDNA binding protein in complex with ssDNA.
abstract: Crystals of the C-terminal domain of the adenovirus single-stranded DNA binding protein (DBP) in complex with the single-stranded oligonucleotide (dT)16 have been obtained by a batch method from material obtained by chymotryptic digest of full-length DBP. The colorless crystals grow as hexagonal prisms to a maximal size of approximately 0.85 x 0.55 x 0.55 mm. The spacegroup is P3(1)21 with unit cell constants a = 151.5 A and c = 124.0 A. There are either three or four molecules of DBP per asymmetrical unit. To improve the reproducibility of crystallization, recombinant protein has been produced using a baculovirus expression system and shown to crystallize under the same conditions.
authors.affiliation: Structural Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: P N
authors.initials: PN
authors.lastname: Kanellopoulos
authors.forename: H
authors.initials: H
authors.lastname: van der Zandt
authors.forename: D
authors.initials: D
authors.lastname: Tsernoglou
authors.forename: P C
authors.initials: PC
authors.lastname: van der Vliet
authors.forename: P A
authors.initials: PA
authors.lastname: Tucker
mesh_terms: D001483:Base Sequence; D002845:Chromatography; D002918:Chymotrypsin / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D011071:Poly T / Q000737:chemistry / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: C095599:DBP protein, Adenovirus; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D014764:Viral Proteins; D011071:Poly T; D002918:Chymotrypsin