Isolation of brefeldin A.
1. A chromatographic method for the isolation of brefeldin A (BFA) from the culture medium of Penicillium brefeldianis has been developed. BFA is adsorbed on a reversed-phase (C18) silica, desorbed by methanol, and the crystalline BFA precipitated from the concentrated aqueous solution. The yield of crystalline BFA is 121 mg/l of culture medium from a sample containing 204 mg/l determined by analytical HPLC. 2. The detection limit of BFA is 50 ng by analytical HPLC and BFA in the mother liquor of crystallization is recovered by preparative HPLC.
Kim
H L
HL
Department of Veterinary Physiology and Pharmacology, Texas A&M University, College Station 77843, USA.
Kochevar
J
J
D000900:Anti-Bacterial Agents / Q000302:isolation & purification*; D000998:Antiviral Agents / Q000302:isolation & purification*; D020126:Brefeldin A; D002851:Chromatography, High Pressure Liquid; D003517:Cyclopentanes / Q000302:isolation & purification*; D018942:Macrolides; D010407:Penicillium / Q000737:chemistry* / Q000378:metabolism; D012680:Sensitivity and Specificity; D012997:Solvents
D000900:Anti-Bacterial Agents; D000998:Antiviral Agents; D003517:Cyclopentanes; D018942:Macrolides; D012997:Solvents; D020126:Brefeldin A