Reconstitution photoactive yellow protein from apoprotein and p-coumaric acid derivatives.
We report reconstitution of photoactive yellow protein (PYP) from apoPYP and p-coumaric acid derivatives. The addition of p-coumaric acid to the apoPYP sample did not result in the recovery of PYP. In contrast, yellow products were obtained by the addition of p-coumaryl thiophenyl ester or p-coumaric anhydride to the apoPYP sample, the absorption spectra of which were indistinguishable from the spectrum of intact PYP. Our findings provide strong evidence that PYP has the p-coumaryl chromophore. This reconstitution technique opens the way for further biophysical studies of PYP using artificial chromophore analogs.
Imamoto
Y
Y
Department of Earth and Space Science, Faculty of Science, Osaka University, Japan.
Ito
T
T
Kataoka
M
M
Tokunaga
F
F
D001059:Apoproteins / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry*; D002841:Chromatiaceae; D003373:Coumaric Acids / Q000737:chemistry*; D020283:Photoreceptors, Microbial; D011422:Propionates; D013057:Spectrum Analysis; D013329:Structure-Activity Relationship
D001059:Apoproteins; D001426:Bacterial Proteins; D003373:Coumaric Acids; D020283:Photoreceptors, Microbial; D011422:Propionates; C050162:photoactive yellow protein, Bacteria; C495469:p-coumaric acid