Characterization and functional dissection of the galectin-1 gene promoter.
The galectin-1 gene encodes a beta-galactoside-binding protein whose overexpression is associated with neoplastic transformation and loss of differentiation. Transient transfection assays of a series of deletions constructs (pGAT) showed that the galectin-1 promoter is highly active in cells both expressing and non-expressing the endogenous gene, and that the basal activity is determined by sequences encompassing the transcription start site (-50/+50). Both an upstream (-50/-26) and a downstream position-dependent (+10/+50) cis-elements are necessary for efficient transcriptional activity and are able to bind nuclear proteins.
Salvatore
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Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Università degli Studi di Napoli Federico II, Italy.
Contursi
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Benvenuto
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Bruni
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Chiariotti
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D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D037483:Galectin 1; D015870:Gene Expression; D006388:Hemagglutinins / Q000096:biosynthesis* / Q000235:genetics*; D037102:Lectins / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping; D017384:Sequence Deletion; D014162:Transfection
D037483:Galectin 1; D006388:Hemagglutinins; D037102:Lectins; D011994:Recombinant Proteins; D015500:Chloramphenicol O-Acetyltransferase