Human pancreatic phospholipase A2 stimulates the growth of human pancreatic cancer cell line.
Phospholipase A2 (PLA2) from human pancreas, designated hPLA2-I, functions as a digestive enzyme. Interestingly, the present study demonstrated that the mature form of hPLA2-I stimulated the growth of a human pancreatic cancer cell line MIAPaCa-2, whereas the pro-form was ineffective. PLA2s from Laticauda semifasciata fraction I, Crotalus adamanteus venom, Streptomyces violaceoruber and bee venom, showed no proliferative effect to the growth of MIAPaCa-2. The Scatchard plot analysis revealed that the MIAPaCa-2 cell had a specific binding site for the mature hPLA2-I. The equilibrium binding constant (Kd) and the maximum binding capacity (Bmax) were 2.6 nM and 0.4 fmol/10(6) cells, respectively. These results suggest that the mature hPLA2-I, but not the pro-form, may function as a growth factor of pancreas carcinoma via the specific binding site.
Hanada
K
K
First Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
Kinoshita
E
E
Itoh
M
M
Hirata
M
M
Kajiyama
G
G
Sugiyama
M
M
D001514:Bee Venoms; D002455:Cell Division / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004792:Enzyme Precursors / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D010179:Pancreas / Q000201:enzymology*; D010190:Pancreatic Neoplasms; D010741:Phospholipases A / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D054467:Phospholipases A2; D011485:Protein Binding; D012910:Snake Venoms; D014407:Tumor Cells, Cultured
D001514:Bee Venoms; D004792:Enzyme Precursors; D012910:Snake Venoms; D010741:Phospholipases A; D054467:Phospholipases A2