Improved clean-up procedure for the high-performance liquid chromatographic assay of clomipramine and its demethylated metabolite in human plasma.
A rapid and selective assay of clomipramine and its metabolite desmethylclomipramine in human plasma, based on high-performance liquid chromatography with UV detection has been developed. The compounds were subjected to solid-phase extraction, using Extrelut 1 cartridges. Recoveries ranged between 88-95% for clomipramine, and 75-80% for desmethylclomipramine. This method has been used for therapeutic monitoring of clomipramine and its metabolite in individuals treated with this drug.
Altieri
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Clinical Biochemistry Department, Istituto Superiore di Sanità, Roma, Italy.
Pichini
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Pacifici
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Zuccaro
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P
D000929:Antidepressive Agents, Tricyclic / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002997:Clomipramine / Q000097:blood*; D006801:Humans; D008745:Methylation; D013056:Spectrophotometry, Ultraviolet
D000929:Antidepressive Agents, Tricyclic; D002997:Clomipramine