title: Inhibition of influenza virus protein synthesis by a plant preparation from Geranium sanguineum L.
abstract: A polyphenolic complex (PC) with antiviral properties has been isolated from the Bulgarian medicinal plant Geranium sanguineum L. A study was undertaken to investigate the effect of PC on virus-specific protein synthesis in influenza virus-infected cells. The expression of viral glycoproteins on the surface of chick embryo fibroblasts infected with virus A/FPV, strain Rostock (H7N1) was suppressed. Virus protein synthesis was selectively inhibited as shown by SDS polyacrylamide gel electrophoresis of 35S-methionine-labelled proteins and proteins immunoprecipitated with monoclonal antibodies. The inhibitory effect was dose-dependent and better pronounced when PC was applied after virus infection. Two variants of influenza virus FPV/Rostock with reduced drug susceptibility were selected. PC affected to a lesser extent the synthesis of viral proteins in cells infected with the variants as compared to the sensitive parental virus.
authors.affiliation: Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.
authors.forename: J
authors.initials: J
authors.lastname: Serkedjieva
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D006801:Humans; D009980:Influenza A virus / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D014764:Viral Proteins / Q000096:biosynthesis*
chemical_list: D000998:Antiviral Agents; D010636:Phenols; D011500:Protein Synthesis Inhibitors; D014764:Viral Proteins