A simple analysis of 5 thinner components in human body fluids by headspace solid-phase microextraction (SPME).
A simple method for the extraction of 5 thinner components from human whole blood and urine, using the headspace solid-phase microextraction (SPME) method is presented. After heating a vial containing the samples with 5 compounds (toluene, benzene, n-butyl acetate, n-butanol and n-isoamyl acetate) at 80 degrees C, a polydimethylsiloxane-coated SPME fiber was exposed to the headspace of the vial to allow adsorption of the compounds. The fiber needle was then injected into a capillary gas chromatography (GC) port. The headspace SPME-GC gave intense peaks for each compound and a low level of background noise was seen only for whole blood. Recovery rates of the 5 compounds by use of the headspace SPME-GC were 50-70%. Reproducibility for headspace SPME-GC data were excellent for both body fluids. The calibration curves showed linearity in the range 2-100 ng/0.5 ml whole blood or urine. The detection limits of each compound were 1.1-2.4 ng/0.5 ml sample. The present results on the analysis of 5 thinner components by headspace SPME-GC suggest its applicability to a number of other volatile compounds in forensic toxicology.
Lee
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XP
Department of Legal Medicine, Showa University School of Medicine, Tokyo, Japan.
Kumazawa
T
T
Sato
K
K
D020001:1-Butanol; D000085:Acetates / Q000097:blood / Q000652:urine; D001554:Benzene / Q000032:analysis; D000440:Butanols / Q000097:blood / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods*; D005554:Forensic Medicine; D006801:Humans; D000439:Pentanols / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results; D012997:Solvents / Q000032:analysis*; D015813:Substance Abuse Detection / Q000379:methods*; D014050:Toluene / Q000097:blood / Q000652:urine
D000085:Acetates; D000440:Butanols; D000439:Pentanols; D012997:Solvents; D014050:Toluene; C006848:butyl acetate; D020001:1-Butanol; D001554:Benzene; C020377:isoamyl acetate