Development of a species-specific probe for Mycobacterium xenopi.
In Europe, Mycobacterium xenopi is a frequently isolated species among opportunist mycobacteria, and represents one of the main agents of pulmonary infection due to non-tuberculous mycobacteria. Conventional identification of mycobacteria is a time-consuming and laborious process. In this study, we propose a rapid and simple method for the identification of M. xenopi using polymerase chain reaction. The amplified product consists of a specific probe, present in all 38 M. xenopi strains tested, which could not be amplified and did not present cross-hybridization with a set of 110 strains belonging to 23 other mycobacterial species. The probe was cloned and sequenced. Comparison with data bases revealed no significant homologies with previously described sequences.
Picardeau
M
M
Centre National de Référence des Mycobactéries, Institut Pasteur, Paris.
Vincent
V
V
D001483:Base Sequence; D015139:Blotting, Southern; D015342:DNA Probes / Q000737:chemistry*; D004269:DNA, Bacterial / Q000737:chemistry*; D004587:Electrophoresis, Agar Gel; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*
D015342:DNA Probes; D004269:DNA, Bacterial