title: Coactosin interferes with the capping of actin filaments.
abstract: Coactosin, a 16 kDa protein associated with the actin cytoskeleton from Dictyostelium discoideum, was purified by an improved method, in which other components of the cytoskeleton were removed. The highly purified coactosin had no effect on the time course of actin polymerization, but when added to actin in presence of capping proteins, coactosin counteracted the capping activity of these proteins. The capping proteins cap32/34 and severin domain 1 retarded actin polymerization, on addition of coactosin to samples containing one of these capping proteins the time course of actin polymerization became close to controls without capping proteins.
authors.affiliation: Max-Planck-Institut für Biochemie, Martinsried, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Röhrig
authors.forename: G
authors.initials: G
authors.lastname: Gerisch
authors.forename: L
authors.initials: L
authors.lastname: Morozova
authors.forename: M
authors.initials: M
authors.lastname: Schleicher
authors.forename: A
authors.initials: A
authors.lastname: Wegner
mesh_terms: D000199:Actins / Q000187:drug effects*; D000818:Animals; D001704:Biopolymers; D004023:Dictyostelium; D005656:Fungal Proteins / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D008840:Microfilament Proteins / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D015800:Protozoan Proteins; D011817:Rabbits
chemical_list: D000199:Actins; D001704:Biopolymers; D005656:Fungal Proteins; D008840:Microfilament Proteins; D015800:Protozoan Proteins; C069272:cap32-34 protein, Dictyostelium; C085568:coactosin protein, Dictyostelium discoideum; C036571:severin protein, Dictyostelium