title: Phosphorylation of beta-lactoglobulin using amino acids as the sole base and nucleophile of the reaction.
abstract: beta-Lactoglobulin was phosphorylated with 20, 40, and 80 mol of POCl3/mol protein in the presence of 4, 5, and 6 molar excess of basic amino acid per mol POCl3. Maximal phosphorylation yields of 5 and 3 mol P/mol protein were achieved when the highest stoichiometries of POCl3/arginine and lysine were used. Proportional high amounts of basic amino acids were also grafted to the protein molecule during its phosphorylation through the phosphoamide bond. Modified proteins displayed increased negative charges and reduced isoelectric points and were monomeric. The phosphorylated and phosphoamidated beta-lactoglobulin showed improved functional properties.
authors.affiliation: Institut National de la Recherche Agronomique, Laboratoire d'Etude des Interactions des Molécules Alimentaires, Nantes, France.
authors.forename: M
authors.initials: M
authors.lastname: Sitohy
authors.forename: J M
authors.initials: JM
authors.lastname: Chobert
authors.forename: T
authors.initials: T
authors.lastname: Haertlé
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004655:Emulsions; D006863:Hydrogen-Ion Concentration; D007782:Lactoglobulins / Q000737:chemistry / Q000378:metabolism*; D010758:Phosphorus / Q000737:chemistry; D017553:Phosphorus Compounds; D010766:Phosphorylation
chemical_list: D000596:Amino Acids; D004655:Emulsions; D007782:Lactoglobulins; D017553:Phosphorus Compounds; D010758:Phosphorus; C013196:phosphoryl chloride