title: Expression of immune regulatory molecules in Epstein-Barr virus-associated nasopharyngeal carcinomas with prominent lymphoid stroma. Evidence for a functional interaction between epithelial tumor cells and infiltrating lymphoid cells.
abstract: Undifferentiated nasopharyngeal carcinomas (UNPC) are characterized by an association with Epstein-Barr virus and an abundant lymphoid stroma. The role of this lymphoid stroma is uncertain but is mostly thought to represent an immune response against viral or tumor antigens. We have analyzed the expression of immune regulatory receptor/ligand pairs in snap-frozen biopsies of 20 UNPCs. All cases were Epstein-Barr virus positive and the virus-encoded latent membrane protein, LMP1, was expressed in 6 cases. By immunohistochemistry, we have demonstrated the expression of CD70 and CD40 in the tumor cells of 16 and 18 cases, respectively. Infiltrating lymphoid cells expressing CD27, the CD70 receptor, and the CD40 ligand were present in all cases. The Bcl-2 protein was detected in 17 cases. Unexpectedly, tumor cells of 5 cases expressed at least one member of the B7 family (CD80, CD86, and B7-3) and many lymphoid cells expressing the corresponding counter-receptor, CD28, were detected in all cases. Interestingly, 5 of 6 LMP1-positive cases also expressed B7, whereas all 14 LMP1-negative cases were also B7 negative. Our results indicate that T cells and carcinoma cells communicate in the microenvironment of UNPCs and suggest that the presence of a lymphoid stroma may be a requirement for UNPC growth at least in certain stages of tumor development.
authors.affiliation: Department of Pathology, University of Birmingham, United Kingdom.
authors.forename: A
authors.initials: A
authors.lastname: Agathanggelou
authors.forename: G
authors.initials: G
authors.lastname: Niedobitek
authors.forename: R
authors.initials: R
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Nicholls
authors.forename: W
authors.initials: W
authors.lastname: Yin
authors.forename: L S
authors.initials: LS
authors.lastname: Young
mesh_terms: D002277:Carcinoma / Q000473:pathology* / Q000821:virology*; D004848:Epithelium / Q000473:pathology / Q000503:physiopathology; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D007107:Immune System / Q000502:physiology*; D008221:Lymphoid Tissue / Q000473:pathology* / Q000503:physiopathology; D009303:Nasopharyngeal Neoplasms / Q000473:pathology* / Q000821:virology*; D010641:Phenotype