Expression of Reg gene in the Syrian golden hamster pancreatic islet regeneration model.
We have reported previously that cellophane wrapping of the hamster pancreas is a stimulus that leads to the induction of duct epithelial cell proliferation, followed by endocrine cell differentiation and new islet formation. Reg is a candidate gene that has been reported to be expressed in regenerating pancreatic islets, suggesting a role in islet growth. We examined Reg gene expression in the cellophane-wrap model by isolating total RNA from hamster pancreata at various times after wrapping. Northern blot analysis using a rat cDNA Reg probe showed no expression of Reg in control non-wrapped hamster pancreas, whereas a strong signal was detected in control wrapped rat pancreas. Using reverse transcription of RNA followed by polymerase chain reaction (PCR) we amplified, isolated and sequenced a 194 base pair product which showed homology to rat Reg in both control and wrapped hamster pancreas. When the PCR product was used as a probe for Northern blot analysis, no signal was detected in control non-wrapped pancreata. In contrast, a strong signal was detected 1 and 2 days after wrapping, which then returned to basal between 4 and 6 days after wrapping. A similar temporal pattern was observed using in situ hybridization to localize the Reg gene. One- and 2-day wrapped but not control pancreas expressed Reg in acinar cells, but not in islets.(ABSTRACT TRUNCATED AT 250 WORDS)
Rafaeloff
R
R
Diabetes Institutes, Department of Internal Medicine, Eastern Virginia Medical School, Norfolk 23510, USA.
Barlow
S W
SW
Rosenberg
L
L
Vinik
A I
AI
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002135:Calcium-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D006224:Cricetinae; D017931:DNA Primers; D018076:DNA, Complementary; D005260:Female; D015870:Gene Expression; D017403:In Situ Hybridization; D007515:Islets of Langerhans / Q000378:metabolism / Q000502:physiology*; D051847:Lithostathine; D008647:Mesocricetus; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D010179:Pancreas / Q000378:metabolism / Q000502:physiology*; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D012038:Regeneration; D012689:Sequence Homology, Nucleic Acid
D002135:Calcium-Binding Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D051847:Lithostathine; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; C497616:Reg1a protein, rat