Piperonyl butoxide mutagenicity in human RSa cells.
Piperonyl butoxide (PB) is used as a pesticide synergist and food additive. Its chemically induced mutagenicity was found in cultured human RSa cells by determination of ouabain-resistant (Ouar) phenotypic mutation, with the highest frequency at the concentration of 0.2 microgram/ml. Moreover, K-ras codon 12 mutations in genomic DNA, analyzed by polymerase chain reaction (PCR) and differential dot-blot hybridization using digoxigenin-labeled probes, were detected in RSa cells 6 days after exposure to PB (0.03-0.40 microgram/ml).
Suzuki
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H
Department of Toxicology, Tokyo Metropolitan Research Laboratory of Public Health, Japan.
Suzuki
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N
D001483:Base Sequence; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D011905:Genes, ras; D006801:Humans; D008969:Molecular Sequence Data; D009153:Mutagens / Q000633:toxicity*; D009154:Mutation; D010042:Ouabain / Q000494:pharmacology; D010641:Phenotype; D010882:Piperonyl Butoxide / Q000633:toxicity*; D016133:Polymerase Chain Reaction
D009153:Mutagens; D010042:Ouabain; D004247:DNA; D010882:Piperonyl Butoxide