Effect of proteolytic and glycolytic enzymes on a factor in Sorghum bicolor that induces mycelial growth in the smut fungus, Sporisorium reilianum.
Proteins obtained from seedling shoots and floral meristems of Sorghum bicolor (L.) Moench cv. NK 1210 induced mycelial growth in the smut fungus, Sporisorium reilianum in vitro. Proteins precipitated with trichloroacetic acid and ammonium sulfate were equally effective as inducers, although there were minor variations in the pattern of mycelial growth. Hydrolysis of the protein fraction with the proteolytic enzyme pronase E resulted in considerable reduction in the proteins' ability to induce mycelial growth. Digestion of the protein fraction with driselase, resulted in a slight enhancement of biological activity. The results suggest that amino sugar moieties in glycoproteins may act as inducers of mycelial growth in Sporisorium reilianum.
Bhaskaran
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S
Soil & Crop Sciences Department, TAES, Texas A&M University, College Station, USA.
Smith
R H
RH
D002523:Edible Grain / Q000737:chemistry / Q000382:microbiology*; D005656:Fungal Proteins; D006026:Glycoside Hydrolases / Q000494:pharmacology; D006133:Growth Substances / Q000302:isolation & purification / Q000494:pharmacology; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D011402:Pronase / Q000494:pharmacology; D014587:Ustilaginales / Q000187:drug effects* / Q000254:growth & development*
D005656:Fungal Proteins; D006133:Growth Substances; D010940:Plant Proteins; D006026:Glycoside Hydrolases; C035212:driselase; D011402:Pronase