Effects of ethanol on peripheral benzodiazepine binding sites in the mouse cerebellum and brain stem.
[3H]PK 11195 binding to the peripheral benzodiazepine binding site was investigated in the brain and liver of mice treated with ethanol (4 g/kg, p.o.) daily for 5 days. In the brain stem, Bmax was decreased by 78% in the ethanol-treated group with unaltered Kd (2 nM). The ethanol-withdrawn group did not differ from the control group in both parameters. In the cerebellum, Bmax was decreased by 74% but the binding affinity increased 5-fold as the Kd decreased from 10 to 2 nM. The ethanol-withdrawn group did not differ significantly from the ethanol-treated group. No changes were observed in the cerebrum and liver. These results further support the idea that [3H]PK 11195 binding may be a useful marker for ethanol consumption. The observed changes in these binding sites may represent a functional adaptive response to the ethanol insult and/or a role in the mediation of the effects of ethanol.
Wong
P T
PT
Department of Pharmacology, Faculty of Medicine, National University of Singapore, Kent Ridge.
Tan
C H
CH
Kwan
M F
MF
Ang
L K
LK
Teo
W L
WL
D000437:Alcoholism / Q000378:metabolism*; D000818:Animals; D001569:Benzodiazepines / Q000378:metabolism*; D001835:Body Weight / Q000187:drug effects; D001933:Brain Stem / Q000187:drug effects / Q000378:metabolism*; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism*; D004195:Disease Models, Animal; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D000431:Ethanol / Q000494:pharmacology*; D007546:Isoquinolines / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011963:Receptors, GABA-A / Q000187:drug effects* / Q000378:metabolism
D007546:Isoquinolines; D011963:Receptors, GABA-A; D001569:Benzodiazepines; D000431:Ethanol; C037850:PK 11195