Influence of aging and neurodegenerative disease on changes in band 3-like proteins in white blood cells.
Fluorescent microspheres were used to measure antibody-induced capping of leukocyte membrane proteins that are immunologically related to band 3, the anion exchanger of the erythrocyte. The degree of capping was found to increase with donor age. Surface labeling and capping characteristics of cells from healthy, age-matched controls were not different from those from patients with Alzheimer's disease, multi-infarct dementia, and Down's syndrome. Immunoblots, however, indicated increased expression and/or breakdown of band 3-like proteins in leukocytes from patients when compared with young and old control donors. These findings emphasize the possible involvement of band 3-like proteins of nucleated cells in aging and disease.
Bosman
G J
GJ
Department of Biochemistry, Faculty of Medicine, University of Nijmegen, The Netherlands.
Steetzel
B D
BD
De Man
A J
AJ
Van Kalmthout
P J
PJ
Visser
F E
FE
De Grip
W J
WJ
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000097:blood*; D000544:Alzheimer Disease / Q000097:blood; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism*; D001782:Blood Donors; D016022:Case-Control Studies; D015161:Dementia, Multi-Infarct / Q000097:blood; D004314:Down Syndrome / Q000097:blood; D006801:Humans; D015151:Immunoblotting; D007962:Leukocytes / Q000378:metabolism*; D008565:Membrane Proteins / Q000097:blood*; D008875:Middle Aged; D009410:Nerve Degeneration / Q000502:physiology*
D001457:Anion Exchange Protein 1, Erythrocyte; D008565:Membrane Proteins