title: Detection of Bartonella (Rochalimaea) henselae bacteremia using BacT/Alert blood culture system.
abstract: Bartonella henselae was isolated from the blood of five febrile immunosuppressed patients using BacT/Alert (Organon Teknika, Durham, NC) automated microbial detection system. An immunofluorescence assay (using 1:1000 dilutions) was used to confirm identification of fastidious, pleomorphic, non-Gram staining, argyrophilic bacilli displaying rachety motility that had been presumptively identified as Bartonella spp. The practicality of identification of Bartonella henselae using goat antisera for use in a routine clinical microbiology laboratory was demonstrated by this study.
authors.affiliation: Department of Microbiology, New York University Medical Center, New York, NY 10016, USA.
authors.forename: P M
authors.initials: PM
authors.lastname: Tierno
authors.forename: K
authors.initials: K
authors.lastname: Inglima
authors.forename: M T
authors.initials: MT
authors.lastname: Parisi
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000150:complications; D000328:Adult; D016917:Angiomatosis, Bacillary / Q000175:diagnosis*; D016470:Bacteremia / Q000382:microbiology*; D001431:Bacteriological Techniques / Q000295:instrumentation*; D001474:Bartonella Infections / Q000175:diagnosis / Q000382:microbiology; D018416:Bartonella henselae / Q000302:isolation & purification*; D004396:Coloring Agents; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D008875:Middle Aged; D013997:Time Factors
chemical_list: D004396:Coloring Agents