Apoptotic cell death of a temperature-sensitive central neuronal cell line.
A neuron-like cell line HS-2, derived from a primary fetal rat (E17) hippocampal cell culture using the temperature-sensitive SV 40 large T antigen, exhibits flat shape and grows well in culture medium with 5% fetal calf serum (FCS) at the permissive temperature (PT, 33.5 degrees C). At the non-permissive temperature (NPT, 38.5 degree C), many, but not all cells, have a neuronal shape with processes. The addition of dibutyryl-cAMP promotes the morphological changes in the cells to a neuron-like shape with long neurite-like processes and the cells exhibit neuron-specific enolase- and glutamic acid decarboxylase-immunoreactivity. Apoptotic cell death also occurs in these cultures at the NPT. DNA fragmentation and chromatin condensation that are characteristic of apoptosis occur within 8 h of being placed at the NPT. By 48 h after being placed at the NPT, the number of surviving cells decreases by 40% in the presence of 5% FCS. This cell line should be useful for investigating the mechanisms of 'programmed cell death' of neurons, which appears to occur during brain development and possibly in CNS degenerative diseases.
Hisanaga
K
K
Department of Neurology, University of California at San Francisco, USA.
Kure
S
S
Bredesen
D E
DE
Ikeda
Y
Y
Kohsaka
S
S
Sharp
F R
FR
D000818:Animals; D000952:Antigens, Polyomavirus Transforming; D017209:Apoptosis; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D004249:DNA Damage; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D051381:Rats; D013696:Temperature
D000952:Antigens, Polyomavirus Transforming; D003994:Bucladesine