Adrenal-specific transgene expression and derivation of conditionally immortal rat adrenocortical cell lines.
Conditional immortalisation of cells is a powerful tool for establishing in vitro models maintaining a differentiated phenotype. We are utilising this approach to derive cell lines that maintain the characteristics of glomerulosa and fasciculata cells of the adrenal cortex. Such cell lines should provide a system in which to study aspects of adrenocortical function that are relevant to hypertension, such as the effects of the renin-angiotensin system on steroidogenesis.
Morley
S D
SD
Centre for Genome Research, Edinburgh, UK.
Viard
I
I
Parker
K L
KL
Mullins
J J
JJ
D000302:Adrenal Cortex / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D030801:Animals, Genetically Modified; D000956:Antigens, Viral; D002454:Cell Differentiation; D002460:Cell Line; D002470:Cell Survival / Q000276:immunology; D015870:Gene Expression; D051379:Mice; D008822:Mice, Transgenic; D009928:Organ Specificity / Q000235:genetics; D010641:Phenotype; D051381:Rats; D011916:Rats, Inbred F344; D013539:Simian virus 40 / Q000276:immunology; D013696:Temperature
D000956:Antigens, Viral