Cloning and sequence analysis of the gene encoding the DNA polymerase I from Mycobacterium tuberculosis.
The polA gene (encoding DNA polymerase I) from Mycobacterium tuberculosis was cloned using an internal gene segment probe generated by PCR amplification of genomic DNA [Mizrahi et al., Gene 136 (1993) 287-290]. The gene encodes a polypeptide 904 amino acids (aa) in length that shares 89% identity with a 911-aa homologue from Mycobacterium leprae. The polypeptide has all of the primary structural elements necessary for DNA polymerase and 5'-3' exonuclease activity, but lacks the motifs required for an associated 3'-5' exonuclease (proofreading) activity.
Huberts
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Department of Hematology, University of the Witwatersrand Medical School, Johannesburg, South Africa.
Mizrahi
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V
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004256:DNA Polymerase I / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000201:enzymology / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D004256:DNA Polymerase I