Absence of anti-idiotypic antibodies in IVIG preparations to autoantibodies of rare autoimmune diseases.
Intravenous immunoglobulins (IVIG) were found to contain anti-idiotypic antibodies against autoantibodies of various autoimmune diseases. We examined commercial IVIG preparations, from three different manufactures, for the presence of autoantibodies and anti-idiotypic antibodies of two rare autoimmune diseases--primary biliary cirrhosis [anti-pyruvate dehydrogenase (PDH) antibodies] and Goodpasture's syndrome (anti-NC1 antibodies). We used ELISA studies as well as immunoblotting and anti-PDH enzyme activity for the detection of anti-PDH antibodies. ELISA and immunofluorescence studies were used for the detection of anti-NC1 antibodies. The presence of anti-idiotypic activity against anti-PDH fragments on Sepharose-bound IVIG [F(ab)2]. Anti-anti-NC1 activity was evaluated employing inhibition ELISA and immunofluorescence studies. The commercial IVIG preparations that were examined did not contain anti-PDH or anti-NC1 antibodies nor anti-idiotypic activity against these autoantibodies. We conclude that commercial IVIG may lack anti-idiotypic activity against rare autoantibodies.
Krause
I
I
Department of Medicine B, Sheba Medical Center, Tel-Hashomer, Israel.
Hacham
S
S
Gilburd
B
B
Damianovitch
M
M
Blank
M
M
Shoenfeld
Y
Y
D019867:Anti-Glomerular Basement Membrane Disease / Q000276:immunology*; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D001323:Autoantibodies / Q000032:analysis / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology; D001327:Autoimmune Diseases / Q000276:immunology*; D003094:Collagen / Q000276:immunology; D024141:Collagen Type IV; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000276:immunology*; D008105:Liver Cirrhosis, Biliary / Q000276:immunology*; D011768:Pyruvate Dehydrogenase Complex / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D001324:Autoantigens; D024141:Collagen Type IV; D016756:Immunoglobulins, Intravenous; D011768:Pyruvate Dehydrogenase Complex; C057112:type IV collagen alpha3 chain; D003094:Collagen