Type beta 1 transforming growth factor is an inhibitor of 3 beta-hydroxysteroid dehydrogenase isomerase in mouse adrenal tumor cell line Y1.
In the Y1 mouse adrenal tumor cell line, the 3 beta-hydroxysteroid dehydrogenase isomerase enzyme (3 beta-HSD) which catalyzes the transformation of 3 beta-hydroxy-5-ene steroids to 3-keto-4-ene steroids is active. The effect of type beta 1 transforming growth factor (TGF beta 1), a potent modulator of adrenocortical differentiated functions, on the 3 beta-HSD enzyme was studied. Four isoforms of 3 beta-HSD yielding proteins of different mobility on SDS-PAGE were previously detected in the mouse; whereas only one form was present in the mouse adrenal, we detected two isoforms in the Y1 cells. An inhibition of the basal enzymatic activity was observed after TGF beta 1 treatment which was correlated with a decrease in 3 beta-HSD protein (both isoforms) and mRNA levels.
Cherradi
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N
CEA, INSERM U 244, DBMS, CENG, Grenoble.
Chambaz
E M
EM
Defaye
G
G
D000306:Adrenal Cortex Neoplasms / Q000201:enzymology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D051379:Mice; D009097:Multienzyme Complexes / Q000037:antagonists & inhibitors*; D011375:Progesterone Reductase / Q000037:antagonists & inhibitors*; D012333:RNA, Messenger / Q000378:metabolism; D013251:Steroid Isomerases / Q000037:antagonists & inhibitors*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
C021425:3 beta-hydroxysteroid oxidoreductase-delta(5) 3-ketosteroid isomerase; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D011375:Progesterone Reductase; D013251:Steroid Isomerases