Characterization of sugar receptor expression by neoglycoproteins in oral and oropharyngeal squamous cell carcinomas.
Recognition of the carbohydrate part of cellular glycoconjugates by sugar receptors like lectins may contribute to biosignaling and interactions between normal and transformed cells. Such recognitions may be essential for establishing phenotypic characteristics in neoplastic cells, including metastasis-associated properties. To evaluate various glycoconjugates in tumor diagnosis and clinical therapy, a panel of 18 biotinylated neoglycoproteins was prepared. This included conjugates of a histochemically inert carrier protein and crucial sugar moieties such as D-glucuronic acid, alpha- and beta-N-acetyl-galactosamine, beta-N-acetyl-glucosamine, melibiose, lactose, maltose, cellobiose, mannose, mannose-6-phosphate, fucose, rhamnose, and xylose. In so doing the diazo derivative of the respective p-aminophenyl glycosides was coupled with galactose, beta-N-acetyl-galactosamine or beta-N-acetyl-glucosamine via an epoxy group-containing aliphatic spacer. Other glycoconjugates used were the proteoglycan heparin and the sulfated fucan fucoidan. Labeling was effected with cyanogen bromide activation and aminoalkylation for specific detection of endogeneous sugar receptors, especially lectins. Tissues studied were paraformaldehyde-fixed, paraffin-embedded surgical biopsies from patients with different stages of squamous cell carcinomas (SCCs) of the oral cavity (n = 16) and oropharynx (n = 17), including three lymph node metastases from oropharyngeal primary tumors. Semiquantitative binding differences of probes to tumor stages were evaluated statistically by the Mann-Whitney U-Wilcoxon rank sum W test. Specific binding of a probe to cytoplasmic and nuclear structures was detected with apparent quantitative differences. Overall, the cytoplasmic compartment revealed a higher intensity of histochemical reaction than did nuclear structures, indicating a comparatively higher density of specific carbohydrate receptors.(ABSTRACT TRUNCATED AT 250 WORDS)
Steuer
M K
MK
Department of Otohinolarynology, University of Regensburg, Germany.
Steuer
M
M
Bonkowsky
V
V
Gabius
H J
HJ
Hofstädter
F
F
D000328:Adult; D000368:Aged; D001665:Binding Sites; D002241:Carbohydrates / Q000737:chemistry; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000648:ultrastructure*; D005260:Female; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D037102:Lectins / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D009959:Oropharyngeal Neoplasms / Q000175:diagnosis / Q000648:ultrastructure*; D009960:Oropharynx / Q000648:ultrastructure*
D002241:Carbohydrates; D006023:Glycoproteins; D037102:Lectins