A meta cleavage pathway for 4-chlorobenzoate, an intermediate in the metabolism of 4-chlorobiphenyl by Pseudomonas cepacia P166.
Bacterial degradation of biphenyl and polychlorinated biphenyls proceeds by a well-studied pathway which produces benzoate and 2-hydroxypent-2,4-dienoate (or, in the case of polychlorinated biphenyls, the chlorinated derivatives of these compounds). Pseudomonas cepacia P166 utilizes 4-chlorobiphenyl for growth and produces 4-chlorobenzoate as a central intermediate. In this study we found that strain P166 further transforms 4-chlorobenzoate to 4-chlorocatechol, which is mineralized by a meta cleavage pathway. Key metabolites which we identified include the meta cleavage product (5-chloro-2-hydroxymuconic semialdehyde), 5-chloro-2-hydroxymuconate, 5-chloro-2-oxopent-4-enoate, 5-chloro-4-hydroxy-2-oxopentanoate, and chloroacetate. Chloroacetate accumulated transiently, and slow but stoichiometric dehalogenation was observed.
Arensdorf
J J
JJ
Environmental Toxicology Graduate Program, University of California, Riverside 92521, USA.
Focht
D D
DD
D001673:Biodegradation, Environmental; D001713:Biphenyl Compounds / Q000737:chemistry / Q000378:metabolism*; D016956:Burkholderia cepacia / Q000378:metabolism*; D002723:Chlorobenzoates / Q000737:chemistry / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D008956:Models, Chemical
D001713:Biphenyl Compounds; D002723:Chlorobenzoates; C040768:4-chlorobenzoic acid; C006983:4-chlorobiphenyl