title: In situ mitral valve stabilization with glutaraldehyde.
abstract: Mitral valve repair in the young rheumatic patient carries a high reoperation rate due to progression of the disease. In an attempt to halt or at least slow down this process, the possibility of fixing in situ the valve tissues with glutaraldehyde was explored. Six weanling sheep underwent tanning of their anterior mitral leaflet for two minutes with 0.5% buffered glutaraldehyde. The non-treated posterior mitral leaflet served as control. The animals were sacrificed at varying intervals between 2.5 and 6 months. At sacrifice, Doppler echocardiography and hemodynamic studies were done. The leaflets were subjected to histopathologic examination and calcium and glutaraldehyde contents were estimated. Glutaraldehyde treatment of the anterior leaflet caused thickening of the cusp and chordae associated with partial devitalization of its core tissue, partial loss of endothelium and intense fibrocellular reaction with abundant elastic fibers without altering its functional integrity. It did not induce calcification. There were no detectable levels of glutaraldehyde at explantation. The posterior mitral leaflets were normal. Although the absence of calcification and partial viability of the tissue are encouraging, it does not necessarily follow that this treatment would arrest progression of the underlying disease. This process may have clinical application in the future, but it is not yet recommended.
authors.affiliation: Department of Cardiovascular Diseases, King Faisal Specialist Hospital & Research Centre, Riyadh, Saudi Arabia.
authors.forename: S P
authors.initials: SP
authors.lastname: Kumar
authors.forename: M L
authors.initials: ML
authors.lastname: Ali
authors.forename: G
authors.initials: G
authors.lastname: Prabhakar
authors.forename: A
authors.initials: A
authors.lastname: Becker
authors.forename: C M
authors.initials: CM
authors.lastname: Duran
mesh_terms: D000818:Animals; D002114:Calcinosis / Q000473:pathology; D002118:Calcium / Q000032:analysis; D002815:Chordae Tendineae / Q000187:drug effects / Q000473:pathology; D004195:Disease Models, Animal; D015150:Echocardiography, Doppler; D004547:Elastic Tissue / Q000187:drug effects / Q000473:pathology; D004727:Endothelium / Q000187:drug effects / Q000473:pathology; D005404:Fixatives; D005500:Follow-Up Studies; D005976:Glutaral / Q000032:analysis / Q000627:therapeutic use*; D006349:Heart Valve Diseases / Q000628:therapy; D006439:Hemodynamics; D008943:Mitral Valve / Q000737:chemistry / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D012214:Rheumatic Heart Disease / Q000628:therapy; D012756:Sheep; D014022:Tissue Survival
chemical_list: D005404:Fixatives; D002118:Calcium; D005976:Glutaral