Intracellular accumulation of ofloxacin-loaded liposomes in human synovial fibroblasts.
In order to incorporate ofloxacin within liposomes, the reverse-phase evaporation technique was carried out. The liposome lipid matrix consisted of dipalmitoylphosphatidylcholine-cholesterol-dihexadecylphosphate (4: 3:4 molar ratio). The liposome formulation presented a mean size of 185 +/- 31 nm and had an encapsulation capacity of 5.3 microliters/mumol. The liposome formulation was able to deliver ofloxacin into McCoy cells in a greater amount (2.6-fold) than the free drug, improving antibiotic accumulation.
Fresta
M
M
Institute of Pharmaceutical and Toxicological Chemistry, Catania, Italy.
Spadaro
A
A
Cerniglia
G
G
Ropero
I M
IM
Puglisi
G
G
Furneri
P M
PM
D002478:Cells, Cultured; D004337:Drug Carriers; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008081:Liposomes; D015242:Ofloxacin / Q000008:administration & dosage* / Q000493:pharmacokinetics; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism*
D004337:Drug Carriers; D008081:Liposomes; D015242:Ofloxacin