title: Localization and quantification of endoplasmic reticulum Ca(2+)-ATPase isoform transcripts.
abstract: The Ca(2+)-adenosinetriphosphatase pump of the sarcoplasmic or endoplasmic reticulum (SERCA) plays a critical role in Ca2+ signaling and homeostasis in all cells and is encoded by a family of homologous and alternatively spliced genes. To understand more clearly the role the different isoforms play in cell physiology, we have undertaken a quantitative and qualitative assessment of the tissue distribution of transcripts encoding each SERCA isoform. SERCA1 expression is restricted to fast-twitch striated muscles, SERCA2a to cardiac and slow-twitch striated muscles, whereas SERCA2b is ubiquitously expressed. SERCA3 is expressed most abundantly in large and small intestine, thymus, and cerebellum and at lower levels in spleen, lymph node, and lung. In situ hybridization analyses revealed SERCA3 transcripts in cells of the intestinal crypt, the thymic cortex, and Purkinje cells in cerebellum. In addition, SERCA3 was expressed abundantly in isolated rat spleen lymphocytes, in various murine lymphoid cell lines, and in primary cultured microvascular endothelial cells. This analysis demonstrates that SERCA3 is expressed selectively in cells in which Ca2+ signaling plays a critical and sensitive role in regulating physiological processes.
authors.affiliation: Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: K D
authors.initials: KD
authors.lastname: Wu
authors.forename: W S
authors.initials: WS
authors.lastname: Lee
authors.forename: J
authors.initials: J
authors.lastname: Wey
authors.forename: D
authors.initials: D
authors.lastname: Bungard
authors.forename: J
authors.initials: J
authors.lastname: Lytton
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D000252:Calcium-Transporting ATPases / Q000235:genetics*; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012519:Sarcoplasmic Reticulum / Q000201:enzymology; D014018:Tissue Distribution
chemical_list: D007527:Isoenzymes; D015335:Molecular Probes; D012333:RNA, Messenger; D000252:Calcium-Transporting ATPases