HIV-1 soluble antigens induced CD8+ cytotoxic T-cell responses in an immunized individual.
In an attempt to determine whether immunization of healthy HIV-1 seronegative individuals with a soluble gp160 candidate vaccine could induce an anti-HIV specific immune response, volunteers were immunized by two injections of a water-in-oil emulsion containing a mixture of gp160 antigen together with selected peptides. Following immunization, lymphocytes were collected and stimulated in vitro with autologous HIV-1-infected cells. The results showed that immunization with soluble HIV-1 envelope was able to generate CD3+ CD8+ CTLs directed to gp160 antigen. The CTL response was restricted to class I molecule HLA-A2. The CTL response was comparable to that elicited by immunization with HIV-1-envelope recombinant vaccinia virus.
Achour
A
A
Université Pierre et Marie Curie, Paris, France.
Moukrim
Z
Z
Picard
O
O
Bizzini
B
B
Burny
A
A
Zagury
D
D
D016915:AIDS Vaccines / Q000276:immunology; D000595:Amino Acid Sequence; D017252:CD3 Complex / Q000096:biosynthesis; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003601:Cytotoxicity Tests, Immunologic; D015686:Gene Products, env / Q000276:immunology*; D019302:HIV Envelope Protein gp160; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology; D011498:Protein Precursors / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014616:Vaccinia virus / Q000276:immunology
D016915:AIDS Vaccines; D017252:CD3 Complex; D015686:Gene Products, env; D019302:HIV Envelope Protein gp160; D010446:Peptide Fragments; D011498:Protein Precursors; D014614:Vaccines, Synthetic