Hemozoin differentially modulates the production of interleukin 6 and tumor necrosis factor in murine malaria.
When murine peritoneal macrophages were loaded in vitro with Plasmodium vinckei hemozoin and stimulated for 24 hours with interferon-gamma and/or Escherichia coli lipopolysaccharide, the production of interleukin 6 (IL-6) was drastically reduced, whereas the secretion of tumor necrosis factor (TNF) was increased. In addition, non-radioactive in situ hybridizations in spleen sections of P. vinckei infected mice showed more TNF than IL-6 gene expression in the red pulp around hemozoin accumulation. These results provide evidence that IL-6 and TNF are differentially modulated by hemozoin and that subsequently, the secretion of IL-6 seems to be independent of the TNF production during murine malaria.
Prada
J
J
Institut für Tropenmedizin, Humboldt Universität, Berlin, Germany.
Malinowski
J
J
Müller
S
S
Bienzle
U
U
Kremsner
P G
PG
D000818:Animals; D002478:Cells, Cultured; D004926:Escherichia coli; D005260:Female; D006420:Hemeproteins / Q000494:pharmacology*; D017403:In Situ Hybridization; D007371:Interferon-gamma / Q000494:pharmacology; D015850:Interleukin-6 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology*; D008288:Malaria / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010860:Pigments, Biological; D010961:Plasmodium; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D006420:Hemeproteins; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010860:Pigments, Biological; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C020753:hemozoin; D007371:Interferon-gamma