title: Generation and characterization of monoclonal antibodies to human type-5 tartrate-resistant acid phosphatase: development of a specific immunoassay of the isoenzyme in serum.
abstract: We have characterized four monoclonal antibodies (mAbs) to the purple ("tartrate-resistant," band 5) acid phosphatase of the human osteoclast (TRAP) and used these to develop a specific serum immunoassay. All four mAbs are of high affinity (Ka = 1-5 x 10(8) L/mol) with a very fast Kassoc (0.2-2.0 x 10(5) L mol-1 s-1) and a moderate Kdissoc (1-3 x 10(-3) s). Two of the mAbs were selected to develop a time-resolved fluorescence immunoassay to measure serum concentrations of TRAP. The mean serum immunoreactive TRAP in a group of healthy premenopausal women and men was 3.7 +/- 1.8 micrograms/L (mean +/- SD) and 3.5 +/- 1.6 micrograms/L, respectively. Significantly higher concentrations of TRAP were found in postmenopausal women (6.3 +/- 2.3 micrograms/L) and in eight patients with Gaucher disease (19.3 +/- 4.7 micrograms/L). Further studies are required to investigate the value of serum TRAP as a marker of bone resorption.
authors.affiliation: SmithKline Beecham Pharmaceuticals, Epsom, Surrey, UK.
authors.forename: P
authors.initials: P
authors.lastname: Chamberlain
authors.forename: J
authors.initials: J
authors.lastname: Compston
authors.forename: T M
authors.initials: TM
authors.lastname: Cox
authors.forename: A R
authors.initials: AR
authors.lastname: Hayman
authors.forename: R C
authors.initials: RC
authors.lastname: Imrie
authors.forename: K
authors.initials: K
authors.lastname: Reynolds
authors.forename: S D
authors.initials: SD
authors.lastname: Holmes
mesh_terms: D000135:Acid Phosphatase / Q000097:blood* / Q000276:immunology*; D000293:Adolescent; D000328:Adult; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000915:Antibody Affinity; D015415:Biomarkers; D001847:Bone Diseases / Q000201:enzymology; D001862:Bone Resorption; D005260:Female; D015200:Fluoroimmunoassay / Q000379:methods* / Q000706:statistics & numerical data; D005776:Gaucher Disease / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000097:blood* / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D017698:Postmenopause; D017697:Premenopause; D011994:Recombinant Proteins / Q000276:immunology; D012680:Sensitivity and Specificity; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D007527:Isoenzymes; D011994:Recombinant Proteins; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase