Sequences of actin implicated in the polymerization process: a simplified mathematical approach to probe the role of these segments.
Regulation of actin polymerization and depolymerization is essential for the functions of actin in non-muscle cells and is mediated by a large number of heterologous actin-binding proteins which questions their true impact on the polymerization process. As a model, we report here the modulating effect of monospecific antibody fragments (Fab) as in vitro effectors on actin polymerization kinetics. Polymerization curves were obtained through fluorescence measurements. They were fitted using analytical equations derived from classical models describing the actin polymerization process with the aim of identifying kinetic steps potentially altered by the effectors. The study was limited to three short segments bore by the 300-328 sequence which is located in actin subdomain 3 and implicated in one of the monomer-monomer interfaces. We observed that antibodies which inhibited actin polymerization reacted with both G- and F-actins, modulated both nucleation and elongation steps, enhanced actin monomer dissociation from the filament and apparently did not act as capping or sequestering proteins. Among the antibody populations specific for a restricted and selected sequence in subdomain 3 of actin (sequence 300-326), only those directed to epitopes located near Met 305 and 325 were effective. In contrast, antibodies directed towards the alpha-helix located between the two preceding epitopes had no effect. All the results analyzed here emphasize the important role of some discrete regions and their conformational state in regulation of the interconversion between monomeric and polymeric actins which could be controlled in different ways by the various actin-binding proteins.
Houmeida
A
A
CNRS, UPR 9008 Centre de Recherches de Biochimie Macromoléculaire, INSERM, U. 249, Université de Montpellier 1, France.
Bennes
R
R
Benyamin
Y
Y
Roustan
C
C
D000199:Actins / Q000737:chemistry* / Q000276:immunology; D000818:Animals; D001665:Binding Sites; D055592:Biophysical Phenomena; D001703:Biophysics; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D000939:Epitopes / Q000737:chemistry; D066298:In Vitro Techniques; D008274:Magnesium / Q000737:chemistry; D008956:Models, Chemical; D015394:Molecular Structure; D008974:Mollusca; D011108:Polymers / Q000737:chemistry; D011487:Protein Conformation; D011817:Rabbits
D000199:Actins; D000939:Epitopes; D011108:Polymers; D008274:Magnesium