title: High-performance liquid chromatographic assay for xanomeline, a specific M-1 agonist, and its metabolite in human plasma.
abstract: A reversed-phase high-performance liquid chromatographic assay (HPLC) was utilized for monitoring xanomeline (LY246708/NNC 11-0232) and a metabolite, desmethylxanomeline, in human plasma. Xanomeline, desmethylxanomeline and internal standard were extracted from plasm with hexane at basic pH. The organic solvent extract was evaporated to dryness with nitrogen and the dried residue was reconstituted with 0.2 M HCl-methanol (50:50, v/v). A Zorbax CN 150 x 4.6 mm I.D., 5-microns column and mobile phase consisting of 0.5% (5 ml/l) triethylamine (TEA) adjusted to pH 3.0 with concentrated orthophosphoric acid-tetrahydrofuran (THF) (70:30, v/v) produced consistent resolution of analytes from endogenous co-extracted plasma components. Column effluent was monitored at 296 nm/0.008 a.u.f.s. and the assay limit of quantification was 1.5 ng/ml. A linear response of 1.5 to 20 ng/ml was sufficient to monitor plasma drug/metabolite concentrations during clinical trials. HPLC assay validation as well as routine assay quality control (QC) samples indicated assay precision/accuracy was better than +/- 15%.
authors.affiliation: Lilly Research Laboratories, Lilly Laboratory for Clinical Research, Eli Lilly and Company, Indianapolis, IN 46202, USA.
authors.forename: S C
authors.initials: SC
authors.lastname: Kasper
authors.forename: P L
authors.initials: PL
authors.lastname: Bonate
authors.forename: A F
authors.initials: AF
authors.lastname: DeLong
mesh_terms: D000544:Alzheimer Disease / Q000097:blood* / Q000188:drug therapy; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D018721:Muscarinic Agonists / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D011725:Pyridines / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013830:Thiadiazoles / Q000097:blood* / Q000493:pharmacokinetics / Q000627:therapeutic use
chemical_list: D018721:Muscarinic Agonists; D011725:Pyridines; D013830:Thiadiazoles; C075257:xanomeline