title: Molecular comparison of the mitochondrial and cytoplasmic hsp70 of Trypanosoma cruzi, Trypanosoma brucei and Leishmania major.
abstract: We compared the expression and localization of the mitochondrial and cytoplasmic hsp70 of the protozoans Trypanosoma cruzi, Trypanosoma brucei and Leishmania major. The mitochondrial protein is encoded by multiple mRNA in all species, while the cytoplasmic protein is encoded by a single mRNA. In all three species, the mitochondrial hsp70 is concentrated in the kinetoplast, a submitochondrial structure that houses the unusual DNA (kDNA) that characterizes this group of organisms, while the cytoplasmic protein is distributed throughout the cell. These results suggest that, in all kinetoplastid species, mt-hsp70 has a specific function in kDNA biology, possibly in the processes of kDNA replication, RNA editing or kinetoplast structure.
authors.affiliation: Department of Microbiology-Immunology, Northwestern University Medical School, Chicago 60611, USA.
authors.forename: K G
authors.initials: KG
authors.lastname: Klein
authors.forename: C L
authors.initials: CL
authors.lastname: Olson
authors.forename: J E
authors.initials: JE
authors.lastname: Donelson
authors.forename: D M
authors.initials: DM
authors.lastname: Engman
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000737:chemistry*; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis* / Q000096:biosynthesis; D018320:Leishmania major / Q000737:chemistry* / Q000378:metabolism; D008928:Mitochondria / Q000737:chemistry*; D015800:Protozoan Proteins / Q000032:analysis* / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D016053:RNA, Protozoan / Q000096:biosynthesis; D014345:Trypanosoma / Q000737:chemistry*; D014346:Trypanosoma brucei brucei / Q000737:chemistry / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000378:metabolism
chemical_list: D018840:HSP70 Heat-Shock Proteins; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan