title: Cloning, expression and immunological characterization of Ory s 1, the major allergen of rice pollen.
abstract: We have isolated and characterized a cDNA clone, Ory s 1, encoding a group-1 allergen of rice pollen. The Ory s 1 protein shows significant sequence identity to the major allergen of rye-grass pollen, Lol p 1. RNA gel blot analysis shows that the Ory s 1 gene is expressed in mature anthers, but not in vegetative or other floral tissues tested. Southern blot analysis indicates that this clone represents a member of a small gene family in rice. Western blot analyses of total rice pollen proteins with the group-1 allergen-specific monoclonal 3A2 and IgE antibodies from grass pollen-allergic patients, revealed the presence of cross-reactive antigenic and allergenic epitopes in Ory s 1.
authors.affiliation: School of Botany, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.forename: P
authors.initials: P
authors.lastname: Theerakulpisut
authors.forename: N
authors.initials: N
authors.lastname: Goulding
authors.forename: C
authors.initials: C
authors.lastname: Suphioglu
authors.forename: M B
authors.initials: MB
authors.lastname: Singh
authors.forename: P L
authors.initials: PL
authors.lastname: Bhalla
mesh_terms: D000485:Allergens / Q000235:genetics* / Q000276:immunology; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003429:Cross Reactions; D018076:DNA, Complementary; D015870:Gene Expression; D017343:Genes, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family; D012275:Oryza / Q000235:genetics*; D018515:Plant Leaves; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D018517:Plant Roots; D011058:Pollen; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D000485:Allergens; D018076:DNA, Complementary; C096821:Ory s I protein, Oryza sativa; D010940:Plant Proteins