Molecular characterization of a Galactomyces geotrichum lipase, another member of the cholinesterase/lipase family.
Geotrichum candidum secretes several lipase isoenzymes, differing in their selectively towards esters of long chain fatty acids with a cis-9 double bond. One group shows an absolute selectively towards these fatty acid esters, the other group has a more relaxed specificity and will also hydrolyze other long chain fatty acid esters. Galactomyces geotrichum secrets a lipase that has the same specificity as the latter group. The corresponding lipase gene was cloned from Galactomyces geotrichum. From an alignment of our enzymes' primary structure with those of different strains of Geotrichum candidum, remarkable conservation is evident and it is not yet possible to identify residues/structures responsible for differences in fatty acid specificity. Comparison of the GCL/GGL family with a variety of lipases from other sources, indicated that they are more related to mammalian than microbial lipases.
Phillips
A
A
Department of Microbiology and Biochemistry, University of the Orange Free State, Bloemfontein, Republic of South Africa.
Pretorius
G H
GH
van Rensburg
H G
HG
D000595:Amino Acid Sequence; D001483:Base Sequence; D002802:Cholinesterases / Q000737:chemistry*; D003001:Cloning, Molecular; D005848:Geotrichum / Q000737:chemistry / Q000235:genetics*; D008049:Lipase / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013379:Substrate Specificity
D008049:Lipase; D002802:Cholinesterases