Effects of amiodarone on beating rate and Na-K-ATPase activity in cultured neonatal rat heart myocytes.
1. The purpose of this study was to examine the possibility that the cellular action of amiodarone is mediated by inhibition of thyroid hormone regulatory functions within the myocardial cell. We measured the rate of cell beating and the activity of Na-K-ATPase in cultured neonatal rat heart myocytes. 2. Amiodarone (0.25 and 1 microgram/ml) reduced beating rate up to 75% within 20 min, and Na-K-ATPase activity up to 40% within 2 hr. No toxic effects were detected in the treated cells. 3. The inhibitory actions of amiodarone on beating rate and Na-K-ATPase activity were the same in myocytes grown in the presence or absence of 3-iodothyronine (T3, 5 nM). 4. These data indicate that amiodarone affects beating rate and Na-K-ATPase activity independently of thyroid hormone. It is suggested that interference of amiodarone with thyroid hormone action is not the only mechanism by which this drug modulates some functions of the myocardial cell.
Bergman
M
M
Hasharon Hospital, Petah-Tikva, Israel.
Cohen
F
F
Schlesinger
H
H
Kessler-Icekson
G
G
D000638:Amiodarone / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D003864:Depression, Chemical; D004347:Drug Interactions; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D006339:Heart Rate / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects* / Q000378:metabolism*; D014284:Triiodothyronine / Q000494:pharmacology
D014284:Triiodothyronine; D000254:Sodium-Potassium-Exchanging ATPase; D000638:Amiodarone