Overexpressing Cu/Zn superoxide dismutase enhances survival of transplanted neurons in a rat model of Parkinson's disease.
A high survival rate of grafted dopamine neurons is crucial for reversing neurological deficits following brain tissue transplantation in Parkinson's disease. For unknown reasons the survival rate of transplanted dopamine neurons is only around 10% in experimental animals. The hypothesis that oxidative stress causes the loss of transplanted neurons was tested by grafting neurons from transgenic mice that overexpress Cu/Zn superoxide dismutase. Compared with the survival of those taken from non-transgenic littermates, the survival was 4 times higher for the transgenic dopamine neurons with a concomitant more extensive functional recovery. The results provide direct support for the free radical hypothesis of dopaminergic neuron death in brain tissue grafting.
Nakao
N
N
Department of Medical Cell Research, University of Lund, Sweden.
Frodl
E M
EM
Widner
H
H
Carlson
E
E
Eggerding
F A
FA
Epstein
C J
CJ
Brundin
P
P
D000662:Amphetamines / Q000494:pharmacology; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D006085:Graft Survival; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology / Q000637:transplantation*; D051379:Mice; D008822:Mice, Transgenic; D010300:Parkinson Disease / Q000628:therapy*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013239:Stereotyped Behavior / Q000187:drug effects; D013378:Substantia Nigra / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism*; D014183:Transplantation, Heterologous
D000662:Amphetamines; D012333:RNA, Messenger; D013482:Superoxide Dismutase