Targeted integration of transfected and infected adeno-associated virus vectors containing the neomycin resistance gene.
Human adeno-associated virus (AAV) integrates specifically into a site (AAVS1) on chromosome 19q13.3-qter. We report that both transfected and infected AAV-vectors will target a foreign gene preferentially to AAVS1. Of 11 infected cell lines studied, nine (82%) showed integration of the neomycin resistance marker into AAVS1, which confirms the findings of previous investigations. We also show site-specific integration (nine out of 12 cell lines, 75%) following transfection of an AAV vector containing the neomycin gene. The ability of transfected AAV sequences to preferentially target and express selectable genes at a known site in the human genome extends the potential use of AAV as a vector in gene therapy.
Shelling
A N
AN
Biochemistry Department, University of Otago, Dunedin, New Zealand.
Smith
M G
MG
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002460:Cell Line; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics; D000229:Dependovirus / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D005819:Genetic Markers; D015316:Genetic Therapy; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D009355:Neomycin / Q000494:pharmacology; D014162:Transfection; D016662:Virus Integration / Q000235:genetics
D000900:Anti-Bacterial Agents; D004274:DNA, Recombinant; D005819:Genetic Markers; D009355:Neomycin