title: Mei-S332, a Drosophila protein required for sister-chromatid cohesion, can localize to meiotic centromere regions.
abstract: Mutations in the Drosophila mei-S332 gene cause premature separation of the sister chromatids in late anaphase of meiosis I. Therefore, the mei-S332 protein was postulated to hold the centromere regions of sister chromatids together until anaphase II. The mei-S332 gene encodes a novel 44 kDa protein. Mutations in mei-S332 that differentially affect function in males or females map to distinct domains of the protein. A fusion of mei-S332 to the green fluorescent protein (GFP) is fully functional and localizes specifically to the centromere region of meiotic chromosomes. When sister chromatids separate at anaphase II, mei-S332-GFP disappears from the chromosomes, suggesting that the destruction or release of this protein is required for sister-chromatid separation.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
authors.forename: A W
authors.initials: AW
authors.lastname: Kerrebrock
authors.forename: D P
authors.initials: DP
authors.lastname: Moore
authors.forename: J S
authors.initials: JS
authors.lastname: Wu
authors.forename: T L
authors.initials: TL
authors.lastname: Orr-Weaver
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002503:Centromere / Q000378:metabolism; D002842:Chromatids / Q000378:metabolism; D016386:Chromosome Walking; D002875:Chromosomes / Q000378:metabolism*; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D005260:Female; D017344:Genes, Insect; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics; D008297:Male; D008540:Meiosis / Q000235:genetics*; D008938:Mitosis / Q000502:physiology; D008969:Molecular Sequence Data; D011485:Protein Binding; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012727:Sex Characteristics; D013090:Spermatocytes / Q000648:ultrastructure
chemical_list: D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D008164:Luminescent Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; C096223:mei-S332 protein, Drosophila; D049452:Green Fluorescent Proteins