Evaluation of a high sodium-low potassium cold-storage solution by the isolated perfused rat kidney technique.
The isolated perfused rat kidney (IPK) model was used to assess initial renal function after 24 h preservation in 3 different cold storage solutions: EuroCollins (EC), a solution prepared according to the formulation of Belzer's solution (High-K+ UW) and a high Na(+)-low K+ Belzer UW solution (High-Na+ UW). GFR and FRNa were measured after 24 h cold storage in each of the solutions during 60 min, and were compared to values obtained in a control group in which renal function was measured immediately after the kidneys had been harvested. ATP and CP were measured in fresh renal tissue, in kidneys preserved for 24 h in each solution, in control IPK, and in reperfused IPK after they had been preserved for 24 h. Main results showed that preservation in either solution caused a dramatic decrease in GFR and in FRNa within the first 60 min following reperfusion of cold-stored kidneys. However FRNa was significantly higher in the High-Na+ UW group. ATP and CP content were decreased to approximately 10% of basal values in all experimental groups after cold-storage. Normothermic reperfusion of IPK after cold-storage induced a restoration of ATP levels, but CP content decreased further. There was no significant difference in ATP and CP content between cold-storage solutions, nor any correlation between metabolic and functional parameters.
Ramella
S G
SG
Department of Renal Functional Exploration, Edouard Herriot Hospital, Lyon, France.
Hadj-Aïssa
A
A
Barbieux
A
A
Steghens
J P
JP
Colpart
J J
JJ
Zech
P
P
Pozet
N
N
D000241:Adenosine / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000378:metabolism; D000493:Allopurinol / Q000737:chemistry; D000818:Animals; D003080:Cold Temperature; D005919:Glomerular Filtration Rate / Q000187:drug effects; D005978:Glutathione / Q000737:chemistry; D006982:Hypertonic Solutions / Q000737:chemistry* / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000737:chemistry; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000503:physiopathology; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D010477:Perfusion; D010725:Phosphocreatine / Q000378:metabolism; D011188:Potassium / Q000737:chemistry*; D011887:Raffinose / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000737:chemistry*
C037096:Euro-Collins' solution; D006982:Hypertonic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D010725:Phosphocreatine; D000493:Allopurinol; D000255:Adenosine Triphosphate; D012964:Sodium; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D011188:Potassium