An expression profile of active genes in human colonic mucosa.
An expression profile of genes active in the human colonic mucosa was obtained by collecting 959 partial sequences from a 3'-directed cDNA library. Seven genes were found to produce mRNA each of which comprised more than 1% of total mRNA. Four of these genes are novel, and are likely to be uniquely expressed in the colonic mucosa, and the other three have been identified as genes for fatty acid binding protein, immunoglobulin lambda chain, and carcinoma-associated antigen GA733-2. In the remaining 952 clones, 310 were composed of 118 species occurred recurrently but less than 1%, and 533 clones appeared only once. Because the 3'-directed cDNA library faithfully represents the mRNA population in the source tissue, these numbers represent the relative activities of the gene expression. Altogether 156 gene species were identified in GenBank, and a significant portion of these genes encode proteins found in Golgi apparatus and lysosomes, chromosome-encoded mitochondrial proteins, cell surface proteins, and components in the protein synthesis machinery. The types and proportions of genes identified is consistent with the known major activities of the colonic mucosa such as mucous protein production, energy-dependent water absorption, and rapid cell proliferation and turnover.
Okubo
K
K
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Yoshii
J
J
Yokouchi
H
H
Kameyama
M
M
Matsubara
K
K
D000368:Aged; D001483:Base Sequence; D003106:Colon; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D015723:Gene Library; D005796:Genes; D006801:Humans; D007413:Intestinal Mucosa / Q000502:physiology*; D008297:Male; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary