Gelatin fragments block adherence of Candida albicans to extracellular matrix proteins.
The adherence of Candida albicans to extracellular matrix proteins may be a critical step in the pathogenesis of candidiasis. Yeast cell adherence to type I and IV collagen, fibronectin and laminin was blocked by peptide fragments from denatured type I collagen (gelatin). Gelatin fragments were obtained by digestion of the reduced protein with trypsin or CNBr. The fragments did not have antifungal properties, presumably inhibiting adherence by blocking receptors (adhesins) on the surface of the fungus. A 10-mer (GQRGVVGLPG) fashioned from the alpha-1 chain of type I collagen reduced adherence by 68%. However, a gelatin peptide possessing 47 amino acids reduced fungal adherence to type I collagen by 100%. Peptides derived from the biocompatible protein gelatin, therefore, may have a potential role in reducing the adherence of the fungus to host proteins.
Klotz
S A
SA
Department of Medicine, University of Kansas School of Medicine, Kansas City 64128, USA.
Smith
R L
RL
D000595:Amino Acid Sequence; D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects*; D003094:Collagen / Q000378:metabolism; D003488:Cyanogen Bromide; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005353:Fibronectins / Q000378:metabolism; D005780:Gelatin / Q000494:pharmacology*; D007797:Laminin / Q000378:metabolism; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology*; D017421:Sequence Analysis; D014357:Trypsin
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D010446:Peptide Fragments; D005780:Gelatin; D003094:Collagen; D014357:Trypsin; D003488:Cyanogen Bromide