Structure of the N-linked oligosaccharides from tridacnin, a lectin found in the haemolymph of the giant clam Hippopus hippopus.
Tridacnin, a glycoprotein lectin, was isolated from the symbiotic marine clam Hippopus hippopus and the structure of its major N-glycan chains determined. Tridacnin contains only N-linked glycans which were quantitatively cleaved by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F. Following purification by anion-exchange HPLC, the structures of the oligosaccharides were established using a combination of electrospray ionisation mass spectrometry, 1H-NMR spectroscopy and linkage analysis. The N-glycans are primarily of the oligomannose type but, in addition, some contain a novel 6-O-Me group on the terminal mannose residue of the chain. The N-glycan chains had the following structures. [formula: see text]
Puanglarp
N
N
Department of Molecular Sciences, James Cook University, Townsville, Queensland, Australia.
Oxley
D
D
Currie
G J
GJ
Bacic
A
A
Craik
D J
DJ
Yellowlees
D
D
D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D002240:Carbohydrate Sequence; D006031:Glycosylation; D006458:Hemolymph / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D008766:Methylmannosides; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry*; D011234:Precipitins / Q000097:blood / Q000737:chemistry* / Q000378:metabolism
D037102:Lectins; D008766:Methylmannosides; D009844:Oligosaccharides; D011234:Precipitins; C010801:tridacnin