Hemoglobin analysis by capillary zone electrophoresis.
The analysis of haemoglobin is routine in medical laboratories for the purpose of assessing blood disorders and related pathologies. Haemoglobin is structurally diverse and possesses many variant forms, some disease-producing. With recent improvements in technology, capillary electrophoresis is now being adapted in the clinical laboratory. This paper describes the analysis of haemoglobin variants using uncoated fused-silica capillaries. The effects of using different buffer salts at different concentrations with different buffer pH values to separate haemoglobin variants in these capillaries are described.
Sahin
A
A
Düzen Laboratories, Ankara, Turkey.
Laleli
Y R
YR
Ortancil
R
R
D002021:Buffers; D004586:Electrophoresis / Q000379:methods*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration
D002021:Buffers; D006454:Hemoglobins