title: Effects of overexpressing folding modulators on the in vivo folding of heterologous proteins in Escherichia coli.
abstract: Interest continues to increase in the use of folding modulators to overcome problems with heterologous protein folding in Escherichia coli. Currently, this approach, though highly successful with a number of individual proteins, remains a somewhat hit-and-miss affair. Ongoing research directed at unraveling the precise role and specificity of these folding modulators should generate a clearer understanding of the potential and limitations of overexpressing folding catalysts in vivo. This will facilitate the development, in the not too distant future, of a more structured and rational approach to improving the folding of heterologous gene products in E. coli.
authors.affiliation: University of Zürich, Switzerland.
authors.forename: J G
authors.initials: JG
authors.lastname: Wall
authors.forename: A
authors.initials: A
authors.lastname: Plückthun
mesh_terms: D010450:Endopeptidases / Q000172:deficiency; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D007536:Isomerism; D018832:Molecular Chaperones; D015202:Protein Engineering; D017510:Protein Folding; D021382:Protein Sorting Signals; D011994:Recombinant Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D018832:Molecular Chaperones; D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D010450:Endopeptidases