Association of serum antibodies against defined epitopes of human papillomavirus L1, E2, and E7 antigens and of HPV DNA with incident cervical cancer.
In order to provide a large-scale evaluation of the association with cervical cancer of antibodies against human papillomavirus (HPV) antigens, sera from 233 patients with primary, untreated cervical cancer and from 157 healthy age- and sex-matched blood donors were analyzed for IgG and IgA antibodies against HPV-derived peptide antigens and against bovine papillomavirus. Several serological responses were strongly associated with cervical cancer, notably the IgG response against the HPV 16 epitopes L1:13 (Relative risk [RR]: 5.3), E2:9 (RR: 2.9), and E7:5 (RR: 4.3), and the IgA response against an HPV 18 E2-derived antigen (245:18, RR: 3.1). HPV DNA in corresponding cervical tumors was analyzed by Southern blotting (SB) and polymerase chain reaction (PCR) in 47 patients. Sixty-six percent of the patients carried HPV DNA as determined by SB, 91% of patients analyzed by PCR. Neither the antibody responses, nor the presence of HPV DNA were significantly associated with the biological properties of the tumors.
Dillner
L
L
Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
Zellbi
A
A
Avall-Lundqvist
E
E
Heino
P
P
Eklund
C
C
Pettersson
C A
CA
Forslund
O
O
Hansson
B G
BG
Grandien
M
M
Bistoletti
P
P
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood*; D052688:Bovine papillomavirus 1 / Q000276:immunology; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008875:Middle Aged; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000276:immunology*; D002583:Uterine Cervical Neoplasms / Q000821:virology*
D000914:Antibodies, Viral; D004279:DNA, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G