title: Participation of the propeptide on procathepsin D activation of human peripheral lymphocytes and neutrophils.
abstract: Flow cytometry was used to test the role of the propeptide of procathepsin D in the activation of human peripheral lymphocytes and neutrophils. A selective inhibition of the activation was achieved by antibodies targeted against the propeptide. Synthetic peptide corresponding to the cathepsin D propeptide had a very similar effect as procathepsin D itself. The interaction of procathepsin D with the cells was blocked by the propeptide as monitored in experiments with fluorescently labeled procathepsin D. Our data indicate that procathepsin D activation of human neutrophils and leukocytes described earlier is mediated through the propeptide of the procathepsin D.
authors.affiliation: Department of Pathology, University of Louisville, Kentucky 40292, USA.
authors.forename: V
authors.initials: V
authors.lastname: Vaclav
authors.forename: V
authors.initials: V
authors.lastname: Jana
authors.forename: F
authors.initials: F
authors.lastname: Martin
mesh_terms: D002402:Cathepsin D / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D004792:Enzyme Precursors / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D019041:L-Selectin / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D016177:Macrophage-1 Antigen / Q000378:metabolism; D008360:Mannosephosphates / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
chemical_list: D004792:Enzyme Precursors; D016177:Macrophage-1 Antigen; D008360:Mannosephosphates; D019041:L-Selectin; C027693:mannose-6-phosphate; C058345:procathepsin D; D002402:Cathepsin D