title: Effect of glutamine supplement and hepatectomy on DNA and protein synthesis in the remnant liver.
abstract: One of the physiological roles of glutamine is as a precursor for DNA synthesis. The aims of this study were to determine (1) whether glutamine-supplemented total parenteral nutrition (TPN) enhanced DNA and protein synthesis in the liver and (2) whether glutamine uptake was increased following partial hepatectomy in rats. Male Donryu rats (n = 59; body weight, 250-275 g) were randomized into four groups: (1) sham operation + standard TPN solution (C-STPN); (2) C + glutamine-supplemented TPN (C-GTPN); (3) 70% partial hepatectomy + STPN (H-STPN); (4) partial hepatectomy + GTPN (H-GTPN). On Day 0, rats underwent either a sham operation or 70% partial hepatectomy and concomitantly were catheterized in the jugular vein. TPN was begun immediately after the surgery. GTPN was isocaloric and isonitrogenous with STPN and 25% of total nitrogen was given as glutamine. On Day 2, the animals were sacrificed after either a continuous infusion of 1-14C-leucine or a bolus i.v. injection of bromodeoxyuridine. The rate of hepatic regeneration was enhanced with glutamine supplementation (H-STPN, 60.8 +/- 1.6% vs H-GTPN, 66.3 +/- 2.0, P < 0.05) due to an increase in protein synthesis in the liver (H-STPN, 134.0 +/- 10.3%/day vs H-GTPN, 160.9 +/- 6.9, P < 0.05) and DNA synthesis in hepatocytes (H-STPN, 23.1 +/- 2.5% vs H-GTPN, 31.4 +/- 2.9, P < 0.05). The uptake of glutamine by the liver was increased following hepatectomy with GTPN supplementation.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: First Department of Surgery, Kurume University, School of Medicine, Fukuoka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Yoshida
authors.forename: T
authors.initials: T
authors.lastname: Yunoki
authors.forename: K
authors.initials: K
authors.lastname: Aoyagi
authors.forename: J
authors.initials: J
authors.lastname: Ohta
authors.forename: N
authors.initials: N
authors.lastname: Ishibashi
authors.forename: T
authors.initials: T
authors.lastname: Noake
authors.forename: T
authors.initials: T
authors.lastname: Kakegawa
mesh_terms: D000818:Animals; D001835:Body Weight; D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis*; D005973:Glutamine / Q000008:administration & dosage*; D006498:Hepatectomy; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D014176:Protein Biosynthesis; D051381:Rats
chemical_list: D005973:Glutamine; D004247:DNA; D001973:Bromodeoxyuridine