PEI6, a new basic secretagogue in rat peritoneal mast cells: characteristics of polyethylenimine PEI6 resemble those of compound 48/80.
1. Polyethylenimine with a molecular weight of 600 (PEI6) was the simplest and the most useful to investigate mast cell-activating mechanisms via pertussis toxin (IAP)-sensitive G protein pathway. 2. IAP, lidocaine, or dibutyryl cyclic AMP were inhibitors of the histamine release induced by PEI6, but anti-allergic drug DSCG, the calcium antagonist, D-600, kinase inhibitors, H-7 and K252a, or the calmodulin inhibitor, W-7 were not. 3. The additive effects of compound 48/80 and PEI6 suggested that the action sites for PEI6 overlapped the binding sites of compound 48/80. 4. Mast cell activation induced by PEI6 was sugar-specifically inhibited by N-acetylglucosamine(Glc-NAc)-specific lectins and/or by sialic acid (Sia)-specific lectins, suggesting that the action sites for PEI6 were glycoproteins having GlcNAc and/or Sia residues. 5. Four glycoproteins seemed to be involved in histamine release, including the IAP-sensitive G-protein pathway.
Suzuki-Nishimura
T
T
Department of Molecular Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.
Oku
N
N
Nango
M
M
Uchida
M K
MK
D000818:Animals; D006636:Histamine Release; D008407:Mast Cells / Q000502:physiology*; D037342:Pertussis Toxin; D011094:Polyethyleneimine / Q000494:pharmacology*; D051381:Rats; D010566:Virulence Factors, Bordetella; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D010566:Virulence Factors, Bordetella; D003189:p-Methoxy-N-methylphenethylamine; D011094:Polyethyleneimine; D037342:Pertussis Toxin