Determination of gabapentin in serum by capillary electrophoresis.
A rapid capillary electrophoresis method for the quantification of gabapentin, a new anticonvulsant drug, in serum was developed. The assay involves derivatization of gabapentin with fluorescamine to provide a chromophore for UV-fluorescence detection. The migration time is about 11 min. The assay was linear between 0 and 20 mg/l. No other therapeutic drugs or amino acids interfered with the gabapentin peak. The relative standard deviation is 2.4% at a mean of 11 mg/l (n = 17). The mean serum level for 52 patients on this drug was 5.2 mg/l with a range of 0-12 mg/l.
Garcia
L L
LL
Department of Pathology, Bowman Gray School of Medicine, Winston-Salem, NC 27157, USA.
Shihabi
Z K
ZK
Oles
K
K
D000085:Acetates / Q000097:blood*; D000588:Amines; D000927:Anticonvulsants / Q000097:blood*; D003509:Cyclohexanecarboxylic Acids; D019075:Electrophoresis, Capillary / Q000379:methods*; D000077206:Gabapentin; D006801:Humans; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D005680:gamma-Aminobutyric Acid
D000085:Acetates; D000588:Amines; D000927:Anticonvulsants; D003509:Cyclohexanecarboxylic Acids; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin