Possible involvement of a ubiquitous and several distinct elements in the transcription regulation of the chicken H3 histone gene family.
We have studied possible modes of transcription regulation of four members (H3-II, H3-III, H3-IV and H3-V) of the twelve chicken H3 genes. Results of transient CAT assays using 5'-truncated mutants of H3-IV, together with those reported previously for H3-II and H3-III, indicated that all these four H3 genes possessed a ubiquitous element, the CCAAT sequence, in addition to several distinct elements. Transient CAT assays using the 5'-extended mutants of these four H3 genes showed that the promoter activities decreased with increasing lengths of the 5'-extended fragments, and that the effects were strong for H3-IV and H3-V, but only moderate for H3-II and H3-III.
Takami
Y
Y
Department of Biochemistry, Miyazaki Medical College, Japan.
Nakayama
T
T
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis / Q000235:genetics; D004262:DNA Restriction Enzymes; D006657:Histones / Q000235:genetics*; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D006657:Histones; D015500:Chloramphenicol O-Acetyltransferase; D004262:DNA Restriction Enzymes