title: Versatile E. coli thioredoxin specific monoclonal antibodies afford convenient analysis and purification of prokaryote expressed soluble fusion protein.
abstract: A recently developed E. coli thioredoxin (Trx) gene fusion expression system has circumvented the difficulties associated with inclusion body formation. Although ample quantities of soluble recombinant protein can be expressed using this system, no universal means of quantifying or purifying the fusion product exists. To facilitate the study of Trx fusion proteins, anti-E. coli Trx monoclonal antibodies (mAb) were generated. Two distinct Trx epitopes were defined by competitive ELISA. Both mAb were capable of detecting Trx fusion proteins by sandwich ELISA, and by immunoblot analysis under reducing and non-reducing conditions. In addition, these mAb enabled purification of Trx fusion proteins by immunoprecipitation, as well as affinity chromatography. This report provides the first description of anti-Trx antibodies. These reagents represent a major advance in the isolation and analysis of prokaryote expressed recombinant Trx fusion proteins.
authors.affiliation: Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: R R
authors.initials: RR
authors.lastname: Dickason
authors.forename: R A
authors.initials: RA
authors.lastname: Edwards
authors.forename: J
authors.initials: J
authors.lastname: Bryan
authors.forename: D P
authors.initials: DP
authors.lastname: Huston
mesh_terms: D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000201:enzymology; D011233:Precipitin Tests; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification*; D013879:Thioredoxins / Q000276:immunology*
chemical_list: D011993:Recombinant Fusion Proteins; D013879:Thioredoxins