Identification of phenobarbital N-glucuronides as urinary metabolites of phenobarbital in mice.
Mice were evaluated for their ability to form phenobarbital N-glucuronides. Following oral administration of [14C]phenobarbital to mice, a radiolabeled phenobarbital metabolite cochromatographed with synthetic standards of phenobarbital N-glucuronides. The phenobarbital N-glucuronides were partially purified from the mouse urine as phenobarbital N-methylglucuronates. The phenobarbital N-methylglucuronates isolated from mouse urine had similar chromatographic and spectroscopic properties as synthetic standards. The diastereomers of phenobarbital N-glucuronides and phenobarbital N-glucosides accounted for 7.8 +/- 2.3% and 1.6 +/- 0.6%, respectively, of the radioactivity excreted in mouse urine in the first 48 hr after dosing. This study indicates that the mouse may be a suitable species to study both N-glucosidation and N-glucuronidation simultaneously as metabolic pathways for barbiturates.
Neighbors
S M
SM
Department of Medicinal Chemistry, School of Pharmacy, Virginia Commonwealth University, Richmond 23298-0540, USA.
Soine
W H
WH
D000818:Animals; D002250:Carbon Radioisotopes; D005965:Glucuronates / Q000652:urine*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010634:Phenobarbital / Q000378:metabolism / Q000652:urine*; D013237:Stereoisomerism
D002250:Carbon Radioisotopes; D005965:Glucuronates; D010634:Phenobarbital