title: Recombinant proteins L and LG: efficient tools for purification of murine immunoglobulin G fragments.
abstract: In order to improve antibody purification methods, recombinant proteins L and LG were tested in the purification of murine monoclonal immunoglobulin G (IgG) and its fragments. After affinity constant evaluation in different buffer systems, high-performance affinity chromatographic columns were prepared by coupling the proteins to Affi-prep 10 resin and tested with eight different murine monoclonal antibodies and their fragments of different isotypes. Affinity chromatographic experiments confirmed radioimmunoassay results showing that protein L bound 75% of the tested antibody fragments whereas protein LG had affinity for all the tested fragments. These results demonstrate that protein LG is the most powerful Ig-binding tool so far described.
authors.affiliation: Biochemical Oncology Laboratories, SORIN Biomedica, Saluggia (VC), Italy.
authors.forename: R
authors.initials: R
authors.lastname: Vola
authors.forename: A
authors.initials: A
authors.lastname: Lombardi
authors.forename: L
authors.initials: L
authors.lastname: Tarditi
authors.forename: L
authors.initials: L
authors.lastname: Björck
authors.forename: M
authors.initials: M
authors.lastname: Mariani
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D007128:Immunoglobulin Fragments / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000302:isolation & purification*; D051379:Mice; D010462:Peptostreptococcus; D011994:Recombinant Proteins / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; C055047:Ig L-binding protein, Peptostreptococcus; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D011994:Recombinant Proteins