[Use of 4 microbiological methods to detect Helicobacter pylori].
The presence of Helicobacter pylori (HPY) was confirmed in 90% of a group of 76 patients with gastric disorders. The methods included the direct urease test, a preparation directly stained by the Gram method, culture and serological study using Cobas Core Anti-H. pylori EIA. High levels of IgG HPY antibodies were found in the serum of patients with gastritis chronica and ulcus duodeni. Urease activity can be observed even after 48 hours. Nevertheless such interpretation of test results requires confirmation by other microbiological methods applied simultaneously. HPY was obtained in 50% of culture. Already one hour after the test started, it was possible to confirm this species on the basis of Rapidec pylori. The presented procedure can be used and performed in a laboratory cooperating effectively with a gastroenterologist.
Gospodarek
E
E
Katedra i Zakład Mikrobiologii AM w Bydgoszczy.
Sieradzka
E
E
Zegarski
W
W
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000032:analysis; D001431:Bacteriological Techniques; D004381:Duodenal Ulcer / Q000382:microbiology; D005260:Female; D005756:Gastritis / Q000382:microbiology; D005767:Gastrointestinal Diseases / Q000382:microbiology*; D016480:Helicobacter pylori / Q000201:enzymology / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008875:Middle Aged; D012698:Serologic Tests; D014510:Urease / Q000378:metabolism
D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D014510:Urease