The regulatory C proteins from different restriction-modification systems can cross-complement.
The BamHI restriction-modification system contains a third gene, bamHIC, which positively regulates bamHIR. Similar small genes from other systems were tested in vivo for their ability to cross-complement. C.BamHI protein was identified, purified, and used to raise polyclonal antibodies. Attempts to detect other C proteins in cell extracts by cross-reactivity with C.BamHI antibodies proved unsuccessful.
Ives
C L
CL
New England Biolabs, Beverly, Massachusetts 01915, USA.
Sohail
A
A
Brooks
J E
JE
D001407:Bacillus / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D002474:Cell-Free System; D003429:Cross Reactions; D015280:DNA Restriction-Modification Enzymes / Q000235:genetics* / Q000276:immunology; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000276:immunology; D005798:Genes, Bacterial / Q000235:genetics*; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D014176:Protein Biosynthesis; D011514:Proteus vulgaris / Q000235:genetics / Q000276:immunology; D013045:Species Specificity; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D015280:DNA Restriction-Modification Enzymes; C096463:BamHIC protein, Bacillus amyloliquefaciens; D015245:Deoxyribonuclease BamHI; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific