title: [Quantitative evaluation of corneal epithelial barrier function in corneal epithelial edema].
abstract: Corneal epithelial barrier function in patients with corneal epithelial edema was evaluated by fluorophotometry. Fluorescein uptake through the intact corneal epithelium was 28.1 +/- 2.7 ng/ml (n = 35, mean +/- SE (standard error), calculated from the standard line) at 30 minutes after instillation of 3 microliters of 0.5% sodium fluorescein (20 minutes after eye washing with 20 ml of BSS PLUS). Fluorescein uptake by the cornea in 7 patients with corneal epithelial edema and 6 patients with corneal stromal edema without epithelial edema were 2,489.7 +/- 1,045.6 ng/ml and 99.3 +/- 25.0 ng/ml, respectively, showing a significant increase when compared to normal corneal epithelium (p < 0.0005). Moreover, in patients with corneal epithelial edema, the corneal epithelial barrier function showed significant decrease in comparison with those having corneal stromal edema without epithelial edema (p < 0.05). These results demonstrate a significant decrease in the corneal epithelial barrier function in the epithelial edema.
authors.affiliation: Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Komuro
authors.forename: N
authors.initials: N
authors.lastname: Yokoi
authors.forename: K
authors.initials: K
authors.lastname: Nishida
authors.forename: S
authors.initials: S
authors.lastname: Kinoshita
mesh_terms: D003315:Cornea / Q000503:physiopathology*; D015715:Corneal Edema / Q000503:physiopathology*; D003319:Corneal Stroma / Q000503:physiopathology; D004848:Epithelium / Q000503:physiopathology; D015590:Fluorophotometry; D006801:Humans