Differential display and cloning of messenger RNAs from the late phase of rat liver regeneration.
Liver regeneration allows for recovery from hepatic injuries and regeneration after partial hepatectomy has been extensively used as a model system to study mitogenesis. Many genes have been identified that are induced in the early growing phase of liver regeneration but only a few genes have been identified that are induced in the later stages of regeneration before growth arrest. We used the newly discovered differential display method to identify several genes that were found to be induced at this later stage. Two of them were analyzed further. DNA sequencing of one of them revealed perfect homology to ribosomal protein S24. The sequence of the other gene did not have extensive homology to any sequence in the databases. These results may suggest a role of these two genes in the growth arrest phase of liver regeneration.
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Pittsburgh Transplantation Institute, University of Pittsburgh School of Medicine, PA 15260, USA.
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D000814:Aniline Compounds; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis* / Q000737:chemistry; D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D012269:Ribosomal Proteins / Q000096:biosynthesis
D000814:Aniline Compounds; D017931:DNA Primers; D015342:DNA Probes; D018076:DNA, Complementary; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C489055:Rps24 protein, rat; C487557:4-trifluoromethylaniline; D004247:DNA