title: [Monitoring of hepatocyte xenotransplantation. Usefulness of various laboratory parameters].
abstract: The authors report a study in which they evaluate the efficacy of some laboratory parameters for monitoring intrasplenic hepatocyte xenotransplantation (mouse to rat) as an alternative to 99Tc-HIDA dynamic scan and histologic exam. Swiss mouse and wistar rat hepatocytes were obtained with collagenase digestion. Wistar male rats were used as recipient and were allocated into three groups: A) omotransplanted rats; B) xenotransplanted rats; C) xenotransplanted and immunosuppressed (Cyclosporin A: 20 mg/kg/daily orally) rats. All rats underwent > 70% hepatectomy. Blood samples were obtained daily from a femoral vein and AST, ALT, ALP, bilirubin, albumin and urea were measured. No statistical differences were observed among groups and the laboratory parameters tested can't be considered a valid technique to xenotransplant rejection monitoring.
authors.affiliation: Istituto di Chirurgia Generale e dei Trapianti d'Organo, Facoltà di Medicina e Chirurgia, Università degli Studi di Napoli Federico II, Napoli.
authors.forename: M
authors.initials: M
authors.lastname: Lorenzo
authors.forename: L
authors.initials: L
authors.lastname: Angrisani
authors.forename: M
authors.initials: M
authors.lastname: Triassi
authors.forename: R
authors.initials: R
authors.lastname: Ventriglia
authors.forename: B
authors.initials: B
authors.lastname: Savino
authors.forename: A
authors.initials: A
authors.lastname: Santoro
authors.forename: E
authors.initials: E
authors.lastname: Di Salvo
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D016014:Linear Models; D008099:Liver / Q000166:cytology*; D008297:Male; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D014183:Transplantation, Heterologous