Development and application of a microneutralization ELISA for the detection of antibodies to bovine respiratory syncytial viruses.
A microneutralization enzyme-linked immunosorbent assay (ELISA) was developed to detect specific antibodies to bovine respiratory syncytial viruses (BRSVs) in cattle sera using a monoclonal antibody to the fusion protein of the virus. Serum from 20 naturally exposed, 24 experimentally infected, and 15 immunized cattle were evaluated using 3 different BRSV isolates. Antibody titers determined with the microneutralization ELISA were compared with those derived from a classical virus neutralization assay, an indirect ELISA, and a fusion inhibition assay. These studies demonstrated a high degree of correlation (usually 0.90) among the assays. Furthermore, the results showed that immunization of cattle with one isolate (subgroup) of BRSV induced antibody responses that cross-reacted with at least 2 disparate isolates. These results document the utility of the microneutralization ELISA in assessing functionally important antibody responses to BRSVs in cattle.
Ellis
J A
JA
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
Hassard
L
L
Morley
P S
PS
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000276:immunology / Q000517:prevention & control; D002459:Cell Fusion; D003429:Cross Reactions; D003588:Cytopathogenic Effect, Viral; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005069:Evaluation Studies as Topic; D007114:Immunization; D009500:Neutralization Tests / Q000379:methods*; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary; D018112:Respiratory Syncytial Virus, Bovine / Q000276:immunology* / Q000472:pathogenicity
D000914:Antibodies, Viral; D000956:Antigens, Viral