Carry-over of mRNA during nuclear transfer in pigs.
This research was designed to evaluate the changes that might occur to protein production after nuclear transfer. Eight-cell stage pig embryo nuclei were transferred to enucleated metaphase II oocytes. Twelve hours after nuclear transfer the nuclear transfer embryos were labeled with L-[35S] methionine and subjected to 1-dimensional polyacrylamide gel electrophoresis. During normal pig embryo development, embryonic RNA is produced by the 4-cell stage and results in the production of a 51 kDa band. This 51 kDa band persists through the 8-cell and compact morula stages. Eight-cell stage blastomeres of reconstituted embryos continued the production of the 51 kDa band even after treatment with alpha-amanitin. Since alpha-amanitin should block new mRNA synthesis, the production of this 51 kDa band is likely the result of a carry-over of mRNA coding for this protein in the blastomere cytoplasm.
Parry
T W
TW
Department of Animal Science, University of Missouri-Columbia 65211, USA.
Prather
R S
RS
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004622:Embryo, Mammalian / Q000648:ultrastructure*; D005260:Female; D053652:Nuclear Transfer Techniques; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism*; D013552:Swine / Q000196:embryology*
D000546:Amanitins; D012333:RNA, Messenger