[Enhancing effects of the supernatant of pulmonary alveolar macrophages stimulated by BCG on proliferation on pulmonary fibroblasts].
The supernatant of pulmonary alveolar macrophages (PAM) stimulated by 7.5-60mg/L of BCG could significantly enhance proliferation of pulmonary fibroblasts (PFB), and the supernatant contained high level of TNF. This result indicated BCG could stimulate PAM to produce TNF in vitro. On the other hand, the supernatant of PAM stimulated by 50mg/L of SiO2 could also enhance proliferation of PFB, but the level of TNF in the supernatant was very low and it demonstrated the ingredient in the supernatant enhancing proliferation was not TNF. TNF showed synergistic effect with this ingredient for enhancing proliferation of PFB. Therefore, TNF produced by tuberculosis bacteria-stimulated PAM might be an important factor enhancing the development of fibrosis of silicotuberculosis.
Zhang
S
S
North China Coal Medical College, Tangshan.
Xu
G
G
Luo
L
L
D000818:Animals; D001500:BCG Vaccine / Q000494:pharmacology*; D002455:Cell Division; D005347:Fibroblasts / Q000166:cytology; D008168:Lung / Q000166:cytology*; D016676:Macrophages, Alveolar / Q000502:physiology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012822:Silicon Dioxide / Q000494:pharmacology; D012830:Silicotuberculosis / Q000503:physiopathology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000502:physiology
D001500:BCG Vaccine; D014409:Tumor Necrosis Factor-alpha; D012822:Silicon Dioxide