Recovery characteristics of a rigid, nonmetallic microdialysis probe for use in an electromagnetic field.
It is well known that metal objects perturb electromagnetic fields. Therefore, a conventional metal microdialysis probe cannot be used to determine the bioeffects of electromagnetic radiation. Using fused-silica tubing, we developed an inexpensive nonmetallic, rigid microdialysis probe for use in electromagnetic radiation research or during magnetic resonance imaging. This probe has a concentric tube design, with the membrane length adjustable to the size of the area to be dialyzed. The probes tested had regenerated-cellulose membranes that were 3 mm in length. This report describes how to make this probe. Average relative recovery rates at flow rates of 2.0, 1.0, and 0.5 microliters/min were 21%, 27%, and 42%, respectively. These rates were slightly lower than the 30%, 42%, and 68% obtained with the commercially available metallic CMA10 microdialysis probe with a 3 mm membrane. This may be due to the fused-silica probe and CMA10 probe being made with different types of dialysis membranes.
Mason
P A
PA
Operational Technologies Corporation, San Antonio, Texas, USA.
Romano
W F
WF
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015306:Biogenic Monoamines / Q000032:analysis; D002404:Catheterization / Q000295:instrumentation; D002482:Cellulose / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004574:Electromagnetic Fields; D004867:Equipment Design; D007031:Hypothalamus / Q000378:metabolism; D007440:Intubation / Q000295:instrumentation; D008297:Male; D008567:Membranes, Artificial; D008670:Metals / Q000737:chemistry; D017551:Microdialysis / Q000295:instrumentation*; D010539:Permeability; D011095:Polyethylenes / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012212:Rheology; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties
D000596:Amino Acids; D015306:Biogenic Monoamines; D008567:Membranes, Artificial; D008670:Metals; D011095:Polyethylenes; D012822:Silicon Dioxide; D002482:Cellulose