The cellular prion protein (PrP) selectively binds to Bcl-2 in the yeast two-hybrid system.
Bcl-2 can rescue neurons from death and might, therefore, exert its action by associating with neuron-specific proteins. Using LexA-Bcl-2 as bait, we find that the cellular prion protein (PrP) interacts with Bcl-2, but not Bax, in the yeast two-hybrid system. Since the PrP gene has been implicated in neurodegenerative disorders, this preliminary observation suggests a potential pathogenic mechanism for these conditions.
Kurschner
C
C
Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110, USA.
Morgan
J I
JI
D017209:Apoptosis / Q000235:genetics; D001665:Binding Sites; D008565:Membrane Proteins / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D009410:Nerve Degeneration; D011233:Precipitin Tests; D011328:Prions / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D015003:Yeasts
D008565:Membrane Proteins; D011328:Prions; D011518:Proto-Oncogene Proteins; D011993:Recombinant Fusion Proteins; D008715:Methionine