DNA fingerprinting by pulsed-field gel electrophoresis to investigate a nosocomial pneumonia caused by Legionella bozemanii serogroup 1.
We typed 18 isolates of Legionella bozemanii obtained from clinical and environmental sources by pulsed-field gel electrophoresis. Each of the unrelated strains showed individual restriction patterns of the genomic DNA when either the SfiI or NotI restriction enzyme was used. One strain isolated from a patient with nosocomial legionellosis and two strains from the corresponding hospital water supply were indistinguishable, arguing for a transmission of L. bozemanii from the water supply to the patient. In conclusion, macrorestriction analysis is a valuable tool for studies of the molecular epidemiology of L. bozemanii.
Lück
P C
PC
Institut für Medizinische Mikrobiologie und Hygiene, Technische Universität Dresden, Germany.
Helbig
J H
JH
Hagedorn
H J
HJ
Ehret
W
W
D003428:Cross Infection / Q000382:microbiology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D007875:Legionella / Q000235:genetics / Q000302:isolation & purification*; D018410:Pneumonia, Bacterial / Q000382:microbiology*
D004269:DNA, Bacterial