The detection of gene mutation in transgenic mice (Muta Mouse) following a single oral dose of 2-acetylaminofluorene.
Transgenic mouse assays, such as Muta Mouse, provide a method to predict the potential target organ carcinogenicity of chemical compounds. As part of a validation study, 2-acetylaminofluorene was administered as a single oral dose of either 50 or 100 mg/kg. The mice were killed 3, 7, 14, 28, 56 or 112 days after this single treatment. Mutation frequencies were determined in liver DNA. The results showed that a mutagenic response was observed in the mice treated at the highest dose (100 mg/kg) and this increase was expressed from 28 up to 112 days after the single exposure.
Brooks
T M
TM
Hazleton Europe, North Yorkshire, UK.
Szegedi
M
M
Rosher
P
P
Dean
S W
SW
D015073:2-Acetylaminofluorene / Q000008:administration & dosage / Q000494:pharmacology*; D000284:Administration, Oral; D000818:Animals; D008099:Liver / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D016296:Mutagenesis / Q000187:drug effects; D015203:Reproducibility of Results
D015073:2-Acetylaminofluorene