Presence of Streptococcus DNA sequence in surgical specimens of gastric cancer.
In Southern blot analysis using Mycoplasma 16S ribosomal DNA (rDNA) as a probe, positive signals were detected in DNA samples from surgical specimens of gastric cancers. The DNA that hybridized to Mycoplasma 16S rDNA was eluted from the gel, cloned and sequenced. The cloned sequence was identical to 16S rDNA of Streptococcus anginosus. In Southern blot analysis with the S. anginosus 16S rDNA fragment as a new probe, positive signals were detected in 9 (20%) out of 43 cases of gastric cancer.
Sasaki
H
H
Genetics Division, National Cancer Center Research Institute, Tokyo.
Igaki
H
H
Ishizuka
T
T
Kogoma
Y
Y
Sugimura
T
T
Terada
M
M
D000230:Adenocarcinoma / Q000382:microbiology* / Q000601:surgery; D001483:Base Sequence; D015139:Blotting, Southern; D015342:DNA Probes; D004269:DNA, Bacterial / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013274:Stomach Neoplasms / Q000382:microbiology* / Q000601:surgery; D013291:Streptococcus / Q000235:genetics*
D015342:DNA Probes; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S