title: A rice membrane calcium-dependent protein kinase is induced by gibberellin.
abstract: A rice (Oryza sativa) seed plasma-membrane calcium-dependent serine/threonine protein kinase (CDPK) has been partially purified. Comparing results in seeds that were treated with and without the plant hormone gibberellin (GA) for 10 min showed that rice CDPK was highly induced by GA. After separating solubilized membrane proteins by sodium dodecyl sulfate-gel electrophoresis, followed by renaturation, a radiolabeled phosphoprotein band of approximately 58 kD was detected, and it was apparently produced by autophosphorylation. There are five aspects of the rice CDPK that show similarity to mammalian protein kinase C (PKC) and to other plant CDPKs: (a) Histone IIIS and PKC peptide-ser25 (19-31) are phosphorylated by rice CDPK. (b) The phosphorylation reaction is strictly dependent on calcium. (c) The activity of the rice CDPK is inhibited by either staurosporine or the PKC inhibitory peptide (19-36). (d) Addition of calmodulin has no effect on the activity of the enzyme; however, the CDPK is inhibited by the calmodulin antagonists trifluoperazine and W-7. (e) The rice CDPK reacts with a mammalian anti-PKC antibody in immunoblotting analysis. However, there is one major difference between the rice CDPK and other CDPKs: the rice CDPK is induced by GA, whereas no mammalian PKC or other plant CDPKs are known to be induced by any hormone.
authors.affiliation: Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA.
authors.forename: M
authors.initials: M
authors.lastname: Abo-el-Saad
authors.forename: R
authors.initials: R
authors.lastname: Wu
mesh_terms: D000818:Animals; D000906:Antibodies; D002147:Calmodulin / Q000037:antagonists & inhibitors; D002462:Cell Membrane / Q000201:enzymology; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004790:Enzyme Induction; D005875:Gibberellins / Q000494:pharmacology*; D007700:Kinetics; D008322:Mammals; D008970:Molecular Weight; D012275:Oryza / Q000201:enzymology*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000032:analysis; D011494:Protein Kinases / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D012639:Seeds / Q000201:enzymology*; D013449:Sulfonamides / Q000494:pharmacology; D014268:Trifluoperazine / Q000494:pharmacology; D017871:Calcium-Calmodulin-Dependent Protein Kinases
chemical_list: D000906:Antibodies; D002147:Calmodulin; D005875:Gibberellins; D011493:Protein Kinase C; D011494:Protein Kinases; D013449:Sulfonamides; D014268:Trifluoperazine; D017871:Calcium-Calmodulin-Dependent Protein Kinases; C017967:W 7