Tyrosine kinase may participate in Ca2+ entry for endothelial nitric oxide production.
We have suggested that cyclopiazonic acid (CPA), a Ca(2+)-ATPase inhibitor, produces nitric oxide (NO) by triggering a Ca(2+)-influx resulting from Ca(2+)-depletion in the endoplasmic reticulum of endothelial cells. The tyrosine kinase inhibitor methyl 2,5-dihydroxycinnamate, while having no effect on relaxations induced by A23187 or Na nitroprusside, did inhibit the CPA-induced relaxation and cyclic GMP formation in rat aorta. Tyrosine kinase may participate in endothelial NO synthesis through activation of a Ca2+ entry mechanism.
Hisayama
T
T
Department of Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
Kida
K
K
Imada
K
K
Moritoki
H
H
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D007211:Indoles / Q000494:pharmacology; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D007211:Indoles; D009599:Nitroprusside; D009569:Nitric Oxide; D011505:Protein-Tyrosine Kinases; D000252:Calcium-Transporting ATPases; D002118:Calcium; C000543:cyclopiazonic acid