Insulin receptor overexpression in 184B5 human mammary epithelial cells induces a ligand-dependent transformed phenotype.
To determine the role of the insulin receptor overexpression in breast epithelial cell transformation, the 184B5 human breast epithelial cell line was transfected with human insulin receptor cDNA. In two cell lines transfected with and overexpressing human insulin receptors (IR) (223.8 and 184.5 ng IR/10(6) cells), but not in untransfected cells, insulin binding and tyrosine kinase activity were elevated, and insulin induced a dose-dependent increase in colony formation in soft agar.
Frittitta
L
L
Department of Medicine, University of California, San Francisco 94115, USA.
Vigneri
R
R
Stampfer
M R
MR
Goldfine
I D
ID
D001665:Binding Sites; D001667:Binding, Competitive; D015153:Blotting, Western; D001940:Breast / Q000166:cytology / Q000378:metabolism*; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D018076:DNA, Complementary / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004848:Epithelium; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D008024:Ligands; D010641:Phenotype; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011863:Radioimmunoassay; D011972:Receptor, Insulin / Q000096:biosynthesis* / Q000235:genetics; D015533:Transcriptional Activation; D014162:Transfection
D018076:DNA, Complementary; D007328:Insulin; D008024:Ligands; D011505:Protein-Tyrosine Kinases; D011972:Receptor, Insulin