Serotonergic sprouting into transplanted C-6 gliomas is blocked by S-100 beta antisense gene.
S-100 beta, a calcium binding protein produced by astrocytes, has been proposed to be a neuronotropic agent. In order to test the tropic effects of S-100 beta in vivo, the technique of cell transplantation was used. C6 glioma cells and C6 cells containing a S-100 beta antisense gene (C6AS) were transplanted into contralateral hippocampi. 5-HT immunoreactive, varicose fibers with a normal appearance penetrated into the glioma mass and were seen in high density around the C6 cell mass. However, 5-HT fibers with enlarged, abnormal varicosities were seen bordering C6AS tissue and were very rarely observed within the C6AS cell mass. Extracellular S-100 beta from normal C6 cells may function as a growth factor on sprouting serotonergic fibers.
Ueda
S
S
Department of Biology, New York University, NY 10003, USA.
Kokotos Leonardi
E T
ET
Bell
J
J
Azmitia
E C
EC
D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics; D017690:Cell Transplantation; D016373:DNA, Antisense; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D005910:Glioma / Q000235:genetics*; D009368:Neoplasm Transplantation; D009412:Nerve Fibers / Q000502:physiology; D009414:Nerve Growth Factors / Q000235:genetics*; D009416:Nerve Regeneration; D051381:Rats; D017207:Rats, Sprague-Dawley; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000235:genetics; D012701:Serotonin / Q000502:physiology*
D002135:Calcium-Binding Proteins; D016373:DNA, Antisense; D009414:Nerve Growth Factors; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D012701:Serotonin