title: Monoclonal antibodies reveal complex structure in the membrane skeleton of Tetrahymena.
abstract: Twelve monoclonal antibodies were raised that are specific for the membrane skeleton of Tetrahymena. Five were directed against T. pyriformis and seven were directed against T. thermophila. Some cross-reactivity between species was found. Each monoclonal antibody recognized one of the three major components of epiplasm, i.e. the bands A, B, and C identified in electrophoretic separations of epiplasmic proteins. It was found, using these antibodies, that the epiplasmic proteins A, B and C have overlapping but independent distributions within the cell.
authors.affiliation: Department of Biological Sciences, University of Iowa, Iowa City 52242, USA.
authors.forename: N E
authors.initials: NE
authors.lastname: Williams
authors.forename: J E
authors.initials: JE
authors.lastname: Honts
authors.forename: V M
authors.initials: VM
authors.lastname: Dress
authors.forename: E M
authors.initials: EM
authors.lastname: Nelsen
authors.forename: J
authors.initials: J
authors.lastname: Frankel
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002462:Cell Membrane / Q000648:ultrastructure; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice / Q000276:immunology; D016253:Microscopy, Immunoelectron; D015800:Protozoan Proteins / Q000032:analysis*; D013769:Tetrahymena pyriformis / Q000166:cytology* / Q000648:ultrastructure; D016808:Tetrahymena thermophila / Q000166:cytology* / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; D015800:Protozoan Proteins