Classification of human peripheral nerve fibre groups by conduction velocity and nerve fibre diameter is preserved following spinal cord lesion.
(1) Single nerve fibre action potentials (APs) of lower sacral nerve roots were recorded extracellularly with two pairs of wire electrodes during an operation in which an anterior root stimulator for bladder control was implanted in 9 humans with a spinal cord lesion and dyssynergia of the urinary bladder. Roots that were not saved and that were used to record from were later used for morphometry. (2) Nerve fibre groups were identified by conduction velocity distribution histograms of single afferent and efferent fibres and partly by nerve fibre diameter distribution histograms, and correlation analysis was performed. Group conduction velocity values were obtained additionally from compound action potentials (CAPs) evoked by electrical stimulation of nerve roots and the urinary bladder. (3) The group conduction velocities and group nerve fibre diameters had the following pair-values at 35.5 degrees C: Spindle afferents: SP1 (65 m/s/13.1 microns), SP2 (51/12.1); touch afferents: T1 (47/11.1), T2 (39/10.1), T3 (27/9.1), T4 (19/8.1); urinary bladder afferents: S1 (41 m/s/-), ST (35/-); alpha-motoneurons: alpha 13 (-/14.4), alpha 12 (65m/s/13.1 microns), alpha 11 (60?/12.1)(FF), alpha 2 (51/10.3)(FR), alpha 3 (41/8.2)(S); gamma-motoneurons: gamma beta (27/7.1), gamma 1 (21/6.6), gamma 21 (16/5.8), gamma 22 (14/5.1); preganglionic parasympathetic motoneurons: (10 m/s/3.7 microns). (4) The values of group conduction velocity and group nerve fibre diameter measured in the paraplegics were very similar to those obtained earlier from brain-dead humans and patients with no spinal cord lesions. Also, the number and the density of myelinated fibres were preserved in the roots. Thus, the classification and identification of nerve fibre groups remained preserved following spinal cord lesion. A direct comparison can thus be made of natural impulse patterns of afferent and efferent nerve fibres between paraplegics (pathologic) and brain-dead humans (supraspinal destroyed CNS, in many respects physiologic). (5) When changing the root temperature from 32 degrees C to 35.5 degrees C, the group conduction velocities changed in the following way in one case: SP2: 40 m/s (32 degrees C) to 50 m/s (35.5 degrees C), S1: 31.3 to 40, ST: 25 to 33.8, M: 12.5 to 13.8; alpha 2: 40 to 50, alpha 3: 33 to 40. The group conduction velocities showed different temperature dependence apart from SP2 fibres and alpha 2-motoneurons. (6) Upon retrograde bladder filling the urinary bladder stretch (S1) and tension receptor afferent (ST) activity levels were undulating and increased.(ABSTRACT TRUNCATED AT 400 WORDS)
Schalow
G
G
Department of Clinical Research, Swiss Paraplegic Centre Nottwil.
Zäch
G A
GA
Warzok
R
R
D000200:Action Potentials / Q000502:physiology; D000328:Adult; D001369:Axons / Q000502:physiology; D002799:Cholinergic Fibers / Q000145:classification* / Q000502:physiology / Q000648:ultrastructure; D004558:Electric Stimulation; D005260:Female; D005726:Ganglia, Parasympathetic / Q000166:cytology / Q000502:physiology; D005727:Ganglia, Spinal / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D008297:Male; D009431:Neural Conduction / Q000502:physiology*; D009475:Neurons, Afferent / Q000502:physiology; D009476:Neurons, Efferent / Q000502:physiology; D010525:Peripheral Nerves / Q000166:cytology / Q000502:physiology*; D013119:Spinal Cord Injuries / Q000473:pathology* / Q000503:physiopathology*; D001743:Urinary Bladder / Q000294:innervation; D014563:Urodynamics / Q000502:physiology