L-type Ca2+ channels mediate joint modulation by gamma-amino-butyric acid and glutamate of [Ca2+]i and neuropeptide secretion in immortalized gonadodropin-releasing hormone neurons.
To examine the role of calcium signaling in the joint modulation of gonadotropin-releasing hormone (GnRH) secretion by gamma-aminobutyric acid (GABA) and glutamate, cytoplasmic calcium ([Ca2+]i) responses to the two transmitters were analyzed in monolayer networks of the GT1-7 line of immortalized GnRH neurons. [Ca2+]i was increased by GABA and the GABAA receptor agonist, muscimol, and these responses were inhibited by the GABAA receptor antagonist, bicuculline. In contrast, the GABAB receptor agonist, baclofen, and the GABAB receptor antagonist, phaclofen, had no effect on basal and GABA- and glutamate-induced Ca2+ levels in GT1-7 neurons. The GABA- and muscimol-induced responses consisted of a spike increase in [Ca2+]i followed by a decrease to a plateau; both the increase and the subsequent decrease in [Ca2+]i depended on agonist concentration. Glutamate, N-methyl-D-aspartate (NMDA), and kainate also increased [Ca2+]i, but were less effective than GABA. GABA-, glutamate-, NMDA-, and kainate-induced [Ca2+]i responses were almost abolished in Ca(2+)-free medium and were markedly attenuated by nifedipine. The [Ca2+]i response to GABA was unaffected by prior application of glutamate, and vice versa. This additive effect of glutamate on the GABA-induced [Ca2+]i response was mimicked by prior or simultaneous application of low (10 mM) KCl. The [Ca2+]i response to simultaneous application of GABA and glutamate was also equal to the sum of the individual responses, whereas the GnRH secretory response was larger. However, the secretory responses to GABA and glutamate applied individually or together, were markedly attenuated by nifedipine.(ABSTRACT TRUNCATED AT 250 WORDS)
Spergel
D J
DJ
Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, MD 20892, USA.
Krsmanovic
L Z
LZ
Stojilkovic
S S
SS
Catt
K J
KJ
D001640:Bicuculline / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels / Q000502:physiology*; D002461:Cell Line, Transformed; D003593:Cytoplasm / Q000378:metabolism; D018698:Glutamic Acid / Q000494:pharmacology*; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D007608:Kainic Acid / Q000494:pharmacology; D009118:Muscimol / Q000494:pharmacology; D016202:N-Methylaspartate / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009479:Neuropeptides / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D015220:Calcium Channels; D009479:Neuropeptides; D009118:Muscimol; D007987:Gonadotropin-Releasing Hormone; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D016202:N-Methylaspartate; D011189:Potassium Chloride; D007608:Kainic Acid; D002118:Calcium; D001640:Bicuculline