Interferon-gamma and interleukin-2 suppress the experimental metastasis of mouse renal adenocarcinoma.
While cytokines such as interferon-alpha (IFN-alpha), interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) are partially effective in the treatment of metastatic renal cell carcinoma, these cytokines have not been investigated for their ability to suppress the development of metastasis. We investigated whether they suppressed experimental pulmonary metastasis by subline-2 of streptozotocin-induced mouse renal adenocarcinoma (MRAC-PM2). IFN-gamma (1 x 10(4) and 1 x 10(5) U/mouse) and IL-2 (1 x 10(4) and 1 x 10(5) U/mouse) suppressed the experimental pulmonary metastasis of MRAC-PM2 in a dose-dependent manner, but 1 x 10(5) IU/mouse of IFN-alpha did not. The combination of 1 x 10(5) U IFN-gamma with 1 x 10(5) U IL-2 had the most powerful inhibitory effect on pulmonary metastasis without any side-effects. In addition, the combination of 1 x 10(5) U IFN-gamma with 1 x 10(5) U IL-2 had a synergistic inhibitory effect on the growth of subcutaneous tumors. These results indicate that either IFN-gamma or IL-2, or their combination, produce a more favorable effect than IFN-alpha on the metastatic development of renal adenocarcinoma.
Masumori
N
N
Department of Urology, Sapporo Medical University, School of Medicine, Japan.
Tsukamoto
T
T
Kumamoto
Y
Y
D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology / Q000556:secondary*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004357:Drug Synergism; D007371:Interferon-gamma / Q000627:therapeutic use*; D007376:Interleukin-2 / Q000627:therapeutic use*; D007680:Kidney Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D008175:Lung Neoplasms / Q000517:prevention & control / Q000556:secondary; D051379:Mice; D008808:Mice, Inbred CBA; D009362:Neoplasm Metastasis / Q000517:prevention & control; D012878:Skin Neoplasms / Q000473:pathology / Q000556:secondary
D004273:DNA, Neoplasm; D007376:Interleukin-2; D007371:Interferon-gamma; D003094:Collagen