Genetic analyses of tattered, an X-linked dominant, developmental mouse mutation.
Tattered (Td) is an X-linked dominant mouse mutation that causes prenatal lethality in affected males. To map the locus, we analyzed 199 normal male and affected female progeny from a backcross of Td and Mus castaneus. Pedigree analysis of these animals suggests a gene order of cen-DXWas70-(Td, DXMit26, Gata1, Tcfe3)-(Cybb, Otc)-tel, where Tcfe3 is a transcription factor homologous to a gene involved in the murine microphthalmia (mi) mutation [Hodgkinson et al. Cell 74, 395-404, 1993]. To evaluate Tcfe3 as a candidate for Td, heterozygous tattered females were crossed to xid males to obtain females in which > 95% of B cells expressed genes solely from the Td X Chromosome (Chr). Fluorescent activated cell sorting (FACS) analysis and Western blotting of isolated splenocytes from Td/xid double heterozygotes rule out Tcfe3 as a likely candidate for the Td mutation.
Merrell
K
K
Integrated Program in Molecular Biology and Cellular Biophysics, Columbia College of Physicians and Surgeons, New York, New York, USA.
Gonzales
J C
JC
Wells
S
S
Calame
K
K
Herman
G E
GE
D000818:Animals; D015153:Blotting, Western; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005260:Female; D005434:Flow Cytometry; D005804:Genes, Lethal; D008040:Genetic Linkage; D006239:Haplotypes; D006579:Heterozygote; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009115:Muridae / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction; D014960:X Chromosome