title: Location of light-repressible, small GTP-binding protein of the YPT/rab family in the growing zone of etiolated pea stems.
abstract: YPT/rab proteins are ras-like small GTP-binding proteins that serve as key regulators of vesicular transport. The mRNA levels of two YPT/rab genes in pea plants are repressed by light, with the process mediated by phytochrome. Here, we examined the mRNA expression and the location of the two proteins, pra2- and pra3-encoded proteins, using monoclonal antibodies. The pra2 and pra3 mRNA levels were highest in the stems of dark-grown seedlings. The corresponding proteins were found in the cytosol and the membranes of the stems. Most of the pra2 protein was in the growing internodes, especially in the growing region, but the pra3 protein was widespread. These results suggest that the pra2 protein is important for vesicular transport in stems, possibly contributing to stem growth in the dark, and that the pra3 protein is important for general vesicular transport. The amounts of pra2 and pra3 proteins decreased with illumination. The decrease in these proteins may be related to the phytochrome-dependent inhibition of stem growth that occurs in etiolated pea seedlings.
authors.affiliation: Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nagano
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: H
authors.initials: H
authors.lastname: Narita
authors.forename: Y
authors.initials: Y
authors.lastname: Asaka
authors.forename: Y
authors.initials: Y
authors.lastname: Sasaki
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003624:Darkness; D005260:Female; D019204:GTP-Binding Proteins / Q000096:biosynthesis*; D015870:Gene Expression; D017343:Genes, Plant; D015151:Immunoblotting; D008027:Light; D051379:Mice; D008807:Mice, Inbred BALB C / Q000276:immunology; D018532:Pisum sativum / Q000378:metabolism*; D010940:Plant Proteins; D018547:Plant Stems; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D020691:rab GTP-Binding Proteins
chemical_list: D000911:Antibodies, Monoclonal; C094511:PRA3 protein, Pisum sativum; D010940:Plant Proteins; D012333:RNA, Messenger; D019204:GTP-Binding Proteins; C094510:PRA2 protein, Pisum sativum; D020691:rab GTP-Binding Proteins