Alteration in liver plasma membrane phospholipids and protein kinase activities during the development of chick embryo.
The changes in phospholipid compositions, membrane fluidity and protein kinase A, protein kinase C, tyrosine and casein kinase activities in chick embryo liver plasma membranes during development have been investigated. The percentage participation of sphingomyelin increased while that of phosphatidylserine decreased during chick embryo development. The alterations in membrane sphingomyelin accompanied an increase of steady-state fluorescence anisotropy (rs) of membrane bilayer. Regression analysis indicated positive linear correlations between the percentage participation of sphingomyelin in total membrane phospholipids and (i) protein kinase C (r = 0.903); (ii) casein kinase (r = 0.936); (iii) protein kinase A (r = 0.850); (iv) tyrosine kinase (r = 0.960) activities. We suggest that sphingomyelin might be an specific activator for all types of protein kinase activities investigation.
Gavrilova
N J
NJ
Institute of Biophysics, Bulgarian Academy of Sciences, Sofia.
Setchenska
M S
MS
Petkova
D H
DH
D000818:Animals; D047388:Casein Kinases; D002642:Chick Embryo / Q000201:enzymology* / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D005314:Embryonic and Fetal Development / Q000502:physiology; D004983:Ethanolamines / Q000032:analysis; D005260:Female; D008099:Liver / Q000196:embryology / Q000201:enzymology* / Q000648:ultrastructure; D008244:Lysophosphatidylcholines / Q000032:analysis; D008563:Membrane Lipids / Q000032:analysis* / Q000737:chemistry; D010718:Phosphatidylserines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis* / Q000737:chemistry; D011493:Protein Kinase C / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D013109:Sphingomyelins / Q000032:analysis; D013997:Time Factors
D004983:Ethanolamines; D008244:Lysophosphatidylcholines; D008563:Membrane Lipids; D010718:Phosphatidylserines; D010743:Phospholipids; D011506:Proteins; D013109:Sphingomyelins; C005448:phosphorylethanolamine; D011494:Protein Kinases; D011505:Protein-Tyrosine Kinases; D047388:Casein Kinases; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C