title: Multiple Haemophilus influenzae strains and strain variants coexist in the respiratory tract of patients with cystic fibrosis.
abstract: To investigate the epidemiology of nontypeable Haemophilus influenzae in the respiratory tract of cystic fibrosis (CF) patients, H. influenzae isolates from sputum specimens of 40 CF patients were analyzed longitudinally for 2 years. The isolates were characterized by analysis of the major outer membrane protein (MOMP) patterns. MOMP variant H. influenzae strains were discriminated from distinct strains by randomly amplified polymorphic DNA analysis of genomic DNA. Multiple H. influenzae strains and MOMP variant strains were isolated from single sputum specimens of 29 patients. In 22 patients, a distinct H. influenzae strain persisted over time (median persistence, 8 months; range 2-24). In general, the appearance of MOMP variant strains did not coincide with the occurrence of exacerbations.
authors.affiliation: Department of Medical Microbiology, University of Amsterdam, Netherlands.
authors.forename: L V
authors.initials: LV
authors.lastname: Möller
authors.forename: A G
authors.initials: AG
authors.lastname: Regelink
authors.forename: H
authors.initials: H
authors.lastname: Grasselier
authors.forename: J E
authors.initials: JE
authors.lastname: Dankert-Roelse
authors.forename: J
authors.initials: J
authors.lastname: Dankert
authors.forename: L
authors.initials: L
authors.lastname: van Alphen
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D003550:Cystic Fibrosis / Q000150:complications / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D005260:Female; D005500:Follow-Up Studies; D014644:Genetic Variation; D006192:Haemophilus Infections / Q000150:complications* / Q000382:microbiology; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012137:Respiratory System / Q000276:immunology*; D013183:Sputum / Q000382:microbiology*; D013997:Time Factors
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial