Identification of a 120-kDa protein associated with aromatic hydrocarbon receptor nuclear translocator.
The aromatic hydrocarbon receptor nuclear translocator (ARNT) is a basic helix-loop-helix-PAS protein which forms a heterodimer with aromatic hydrocarbon receptor (AHR), this heterodimer mediating the signal transduction in response to the various xenobiotics such as 2,3,7,8-tetrachlorodibenzo-p-dioxin and directly interacting with target genes by binding to xenobiotic responsive elements. An anti-ARNT antibody was raised in rabbits against the bacterially expressed ARNT of amino acids 21-328 from the N-terminal. Using this antibody, besides ARNT itself, we detected at least one protein, 120 kDa, in the immunoprecipitate of anti-ARNT antibodies in HepG2 cells as well as in Hepa-1 cells. However, this protein is not present in the immunoprecipitate of the anti-AHR antisera nor in that of the preimmune sera of the rabbits used for the immunization.
Hossain
A
A
Department of Molecular Genetics, Tohoku University, Sendai, Japan.
Kikuchi
H
H
Ikawa
S
S
Sagami
I
I
Watanabe
M
M
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D008113:Liver Neoplasms; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D008970:Molecular Weight; D000072317:Polychlorinated Dibenzodioxins / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D011817:Rabbits / Q000276:immunology; D018336:Receptors, Aryl Hydrocarbon / Q000096:biosynthesis / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects; D014157:Transcription Factors / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry; D014407:Tumor Cells, Cultured; D015262:Xenobiotics / Q000494:pharmacology
C497396:ARNT protein, human; D000906:Antibodies; D017931:DNA Primers; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; D015262:Xenobiotics; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator