AmpliType PM and HLA DQ alpha typing from pap smear, semen smear, and postcoital slides.
Deoxyribonucleic acid (DNA) samples extracted from stained pap smear, semen smear and postcoital slides were amplified by the polymerase chain reaction (PCR) and typed for the AmpliType PM and HLA DQ alpha alleles. HLA DQ alpha and PM types consistent with blood controls from the donors were obtained. Stained cells fixed to slides can provide a valuable source of material for determining a genetic profile of the sample donor, particularly in sexual assault cases and possibly in missing person cases involving women.
Roy
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R
N.S.P. Criminalistics Laboratory, Lincoln, NE, USA.
Reynolds
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R
D003075:Coitus; D004247:DNA / Q000032:analysis*; D005260:Female; D005554:Forensic Medicine / Q000379:methods*; D005802:Genes, MHC Class II; D006683:HLA-DQ Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D065006:Papanicolaou Test; D016133:Polymerase Chain Reaction; D011902:Rape / Q000175:diagnosis*; D012661:Semen / Q000737:chemistry*; D014626:Vaginal Smears
D006683:HLA-DQ Antigens; D004247:DNA