Identification of denatured double-stranded DNA by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
We created double-stranded DNA (dsDNA) by annealing synthetic oligonucleotides and used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to measure the difference in molecular weight of the complementary strands after denaturation. Refined sample preparation and deliberate rectilinear construction at a linear mass spectrometer produced a mass resolution exceeding 1000 for single-stranded DNA. We present methodologies and demonstrate the potential for analysing dsDNA by determining the mass of the complementary strands.
Benner
W H
WH
Lawrence Berkeley Laboratory, University of California, Berkeley 94720, USA.
Horn
D
D
Katz
J
J
Jaklevic
J
J
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000032:analysis
D009841:Oligonucleotides; D004247:DNA