Interaction of the MvaI and SsoII methyltransferases with DNAs altered at the central base pair of the recognition sequence.
The interaction of the MvaI and SsoII DNA methyltransferases (MTases; M.MVaI and M.SsoII, respectively) with a set of synthetic DNA duplexes, containing a M.MvaI and M.SsoII recognition site (CCWGG), was investigated. In these DNA duplexes dA or dT of the recognition site was replaced by nucleoside analogs with modified sugar moieties and heterocyclic bases (2'-deoxy-2'-fluorouridine (flU), 1-(beta-D-2'-deoxy-threo-pentofuranosyl)thymine (xT), 1-(beta-D-3'-deoxy-threo-pentofuranosyl)uracil (tU)), or by 1,3-propanediol (Prd). A new approach for monitoring methylation of each strand of DNA duplexes by MTases was developed. It allowed the determination of the influence of the modification in one DNA strand on the methylation of the other. In most cases, for both M.MvaI and M.SsoII, sugar analog-containing duplexes showed inhibition of methylation of only the modified strand. Prd-containing DNA duplexes were not substrates for M.MvaI. M.SsoII did not methylate DNA duplexes in which the dT residue was replaced by Prd.
Brevnov
M G
MG
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
Kubareva
E A
EA
Romanova
E A
EA
Volkov
E M
EM
Karyagina
A S
AS
Nikolskaya
I I
II
Gromova
E S
ES
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D015257:DNA-Cytosine Methylases / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D009705:Nucleosides / Q000737:chemistry; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity
D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D004247:DNA; C029528:DNA modification methylase EcoRII; C063974:DNA modification methylase SsoII; D015257:DNA-Cytosine Methylases