title: Cell culture-PCR technique for detection of infectious cytomegalovirus in peripheral blood.
abstract: We determined the efficacy of PCR for the direct detection of infectious cytomegalovirus (CMV) in specimen-inoculated MRC-5 tube cultures (cell culture-PCR [CC-PCR]). Parallel testing was performed by a shell vial assay-indirect immunofluorescence assay (SVA-IFA) and isolation (conventional MRC tube cultures [TC-CPE]. The sensitivity, specificity, and positive and negative predictive values for CC-PCR, SVA-IFA, and TC-CPE were 81, 99, 94, and 94%, 86, 100, 100, and 96%, and 77, 100, 100, and 93%, respectively. Future application of CC-PCR may be directed toward its use as a screening tool for cytomegalovirus genotypic variants.
authors.affiliation: Jane and Dayton Brown and Dayton T. Brown Jr., Virology Laboratory, Department of Medicine, North Shore University Hospital, Manhasset, New York 11030, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Lipson
authors.forename: A B
authors.initials: AB
authors.lastname: Ashraf
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.forename: M H
authors.initials: MH
authors.lastname: Kaplan
authors.forename: D H
authors.initials: DH
authors.lastname: Shepp
mesh_terms: D001769:Blood / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000821:virology; D003588:Cytopathogenic Effect, Viral; D003951:Diagnostic Errors; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique / Q000706:statistics & numerical data; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D014766:Viremia / Q000175:diagnosis / Q000821:virology; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data