title: The alpha-dystroglycan-beta-dystroglycan complex. Membrane organization and relationship to an agrin receptor.
abstract: Aberrant expression of the dystrophin-associated protein complex is thought to underlie the pathogenesis of Duchenne dystrophy, Becker muscular dystrophy, and severe childhood autosomal recessive muscular dystrophy. Recently, our laboratory identified an agrin receptor from Torpedo electric organ postsynaptic membranes. It is a heteromer of 190- and 50-kDa subunits with similarity to two components of the dystrophin-associated protein complex of alpha- and beta-dystroglycan. We now confirm the relationship between the Torpedo agrin receptor and mammalian dystroglycans and provide further information about the structure of the alpha-dystroglycan-beta-dystroglycan complex. The sequences of three peptides from each Torpedo subunit were 69% identical to mammalian dystroglycans. An antiserum to mammalian beta-dystroglycan recognizes the Torpedo 50-kDa polypeptide. Additionally, like alpha-dystroglycan, the 190-kDa agrin receptor subunit binds laminin. Previous studies have indicated that alpha- and beta-dystroglycan arise by cleavage of a precursor protein. Tryptic peptide mapping of both subunits and amino-terminal sequencing of Torpedo beta-dystroglycan indicate a single cleavage site, corresponding to serine 654 of the mammalian dystroglycan precursor. Gel electrophoresis analysis indicates there is at least one intrachain disulfide bond in beta-dystroglycan. These results provide precise primary structures for alpha- and beta-dystroglycan.
authors.affiliation: Neurobiology Group, Worcester Foundation for Biomedical Research, Shrewsbury, Massachusetts 01545, USA.
authors.forename: K A
authors.initials: KA
authors.lastname: Deyst
authors.forename: M A
authors.initials: MA
authors.lastname: Bowe
authors.forename: J D
authors.initials: JD
authors.lastname: Leszyk
authors.forename: J R
authors.initials: JR
authors.lastname: Fallon
mesh_terms: D018171:Agrin / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D003598:Cytoskeletal Proteins / Q000737:chemistry* / Q000276:immunology; D049030:Dystroglycans; D016189:Dystrophin / Q000737:chemistry; D004557:Electric Organ / Q000737:chemistry; D007797:Laminin / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000737:chemistry* / Q000276:immunology; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011498:Protein Precursors / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D051381:Rats; D017978:Receptors, Growth Factor / Q000737:chemistry; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014101:Torpedo
chemical_list: D018171:Agrin; D003598:Cytoskeletal Proteins; D016189:Dystrophin; D007797:Laminin; D008562:Membrane Glycoproteins; D011498:Protein Precursors; D017978:Receptors, Growth Factor; C080030:agrin receptor; D049030:Dystroglycans