Extracellular guanosine and guanosine-5'-triphosphate increase: NGF synthesis and release from cultured mouse neopallial astrocytes.
Cultures of neonatal mouse cortical astrocytes synthesized NGF mRNA and released immunoreactive NGF (ir-NGF) into the culture medium. Addition of 10 microM guanosine or GTP to the cultures increased ir-NGF release by 6 and 2 fold, respectively, after 24 h, and increased NGF mRNA 6 fold after 4 h and 2-3 fold after 24 h. In contrast, neither adenosine nor ATP (each 1-100 microM) affected either NGF mRNA synthesis or ir-NGF release.
Middlemiss
P J
PJ
Department of Biomedical Sciences, McMaster University, Hamilton, Ont., Canada.
Gysbers
J W
JW
Rathbone
M P
MP
D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005917:Globus Pallidus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006151:Guanosine / Q000494:pharmacology*; D006160:Guanosine Triphosphate / Q000494:pharmacology*; D051379:Mice; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D013268:Stimulation, Chemical
D009414:Nerve Growth Factors; D012333:RNA, Messenger; D006151:Guanosine; D006160:Guanosine Triphosphate