Carp macrophages and neutrophilic granulocytes secrete an interleukin-1-like factor.
Carp, Cyprinus carpio L, macrophages and neutrophilic granulocytes obtained from pronephros were cultured. Supernatant was harvested after 48 h and tested for interleukin-1 (IL-1) bioactivity. A concentration-dependent stimulation of proliferation was found of carp Ig- lymphocytes as well as of the murine IL-1 dependent D10 (N4) M cell line. A 4 h treatment of cells with phorbol myristate acetate prior to culture gave a two- to fourfold enhancement of the bioactivity in the supernatant. Antibodies raised in sheep against human recombinant IL-1 alpha or IL-1 beta added to the supernatant annulled the IL-1 bioactivity. Western blot analysis of supernatants with sheep or rabbit polyclonal antisera against human IL-1s revealed 22 kDa and 15 kDa protein species. The predominant newly synthesized protein that was immunoprecipitated with these antisera was a 15 kDa molecular species. We conclude that carp macrophages and neutrophilic granulocytes produce an IL-1-like molecule with T-cell proliferating potency that shares structural similarities with mammalian IL-1. This is the first evidence for the IL-1 signal protein in carp immunocompetent cells.
Verburg-van Kemenade
B M
BM
Department of Experimental Animal Morphology and Cell Biology, Agricultural University, Wageningen, The Netherlands.
Weyts
F A
FA
Debets
R
R
Flik
G
G
D000818:Animals; D002347:Carps / Q000097:blood / Q000276:immunology*; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007375:Interleukin-1; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects; D008221:Lymphoid Tissue / Q000166:cytology; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D009504:Neutrophils / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013045:Species Specificity; D013601:T-Lymphocytes / Q000187:drug effects
D016207:Cytokines; D007375:Interleukin-1; D010835:Phytohemagglutinins