The release of glutamate and accumulation of intracellular calcium in the guinea pig hippocampal slices during glucose deprivation.
Simultaneous recordings of the time course of change in the extracellular glutamate concentration ([Glu]O) and intracellular Ca2+ level [Ca2+]i during glucose deprivation were performed in the CA3 area of hippocampal slices from guinea pig. Direct measurement of [Glu]O with a glutamate sensitive sensor revealed that [Glu]0 showed a biphasic increase during glucose deprivation and decreased rapidly after re-introduction of glucose in the perfusing medium. The initial increase of [Glu]O preceded [Ca2+]i increase and thereafter a sustained increase of [Glu]O was observed concomitant with massive [Ca2+]i accumulation. Replacement of Na with Li markedly attenuated the rapid reduction of [Glu]O, suggesting that the reduction of [Glu]O was due to Na-dependent glutamate uptake system, which worked immediately after energy recovery.
Takata
T
T
Department of Physiology, Kobe University, School of Medicine, Japan.
Hirai
H
H
Shigemoto
T
T
Okada
Y
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D005947:Glucose / Q000172:deficiency*; D018698:Glutamic Acid / Q000378:metabolism*; D006168:Guinea Pigs; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism*; D009994:Osmolar Concentration
D018698:Glutamic Acid; D005947:Glucose; D002118:Calcium