Two newly established cell lines derived from the same colonic adenocarcinoma exhibit differences in EGF-receptor ligand and adhesion molecule expression.
Two morphologically distinct cell lines, GP2d and GP5d, derived from the same adenocarcinoma of the colon, have been established and characterised. Both clones have the same genetic changes, consistent with the usual pattern of tumour progression in colon cancer. The cells also have an inverted duplication of bands 10q11 to 10q21, but Southern blot analysis failed to identify any translocations involving the ret protooncogene, which maps to this region. GP2d grew by spreading from the edges of microcolonies to form a confluent layer of cells. GP5d grew in discrete islands of cells forming multi-layered colonies. These differing patterns of growth correlated with variation in expression or cellular distribution of alpha 2-integrin, desmoplakin and e-cadherin. Only GP2d responded to exogenously added epidermal growth factor (EGF), transforming growth factor-alpha (TGF alpha) or insulin with an increase in cell numbers, even though both cell lines possessed similar numbers of EGF receptors. Analysis of EGF receptor ligand expression showed that GP5d cells expressed relatively more TGF alpha mRNA than did GP2d; in contrast, amphiregulin mRNA, which was abundant in GP2d, was virtually undetectable in GP5d. Even though GP5d failed to exhibit a growth response to EGF, it underwent a marked epithelial-mesenchymal transition when treated with EGF, indicating separation of growth and morphological responses to EGF.
Solic
N
N
CRC Medical Oncology Unit, Southampton General Hospital, UK.
Collins
J E
JE
Richter
A
A
Holt
S J
SJ
Campbell
I
I
Alexander
P
P
Davies
D E
DE
D000230:Adenocarcinoma / Q000737:chemistry* / Q000473:pathology; D001483:Base Sequence; D015815:Cell Adhesion Molecules / Q000032:analysis*; D002455:Cell Division; D003110:Colonic Neoplasms / Q000737:chemistry* / Q000473:pathology; D004815:Epidermal Growth Factor / Q000032:analysis / Q000235:genetics / Q000494:pharmacology; D066246:ErbB Receptors / Q000032:analysis*; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D016211:Transforming Growth Factor alpha / Q000032:analysis / Q000235:genetics; D014407:Tumor Cells, Cultured
D015815:Cell Adhesion Molecules; D008024:Ligands; D012333:RNA, Messenger; D016211:Transforming Growth Factor alpha; D004815:Epidermal Growth Factor; D066246:ErbB Receptors