title: Simultaneous identification of bacterial virulence genes by negative selection.
abstract: An insertional mutagenesis system that uses transposons carrying unique DNA sequence tags was developed for the isolation of bacterial virulence genes. The tags from a mixed population of bacterial mutants representing the inoculum and bacteria recovered from infected hosts were detected by amplification, radiolabeling, and hybridization analysis. When applied to a murine model of typhoid fever caused by Salmonella typhimurium, mutants with attenuated virulence were revealed by use of tags that were present in the inoculum but not in bacteria recovered from infected mice. This approach resulted in the identification of new virulence genes, some of which are related to, but functionally distinct from, the inv/spa family of S. typhimurium.
authors.affiliation: Department of Infectious Diseases and Bacteriology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
authors.forename: M
authors.initials: M
authors.lastname: Hensel
authors.forename: J E
authors.initials: JE
authors.lastname: Shea
authors.forename: C
authors.initials: C
authors.lastname: Gleeson
authors.forename: M D
authors.initials: MD
authors.lastname: Jones
authors.forename: E
authors.initials: E
authors.lastname: Dalton
authors.forename: D W
authors.initials: DW
authors.lastname: Holden
mesh_terms: D000818:Animals; D001483:Base Sequence; D004251:DNA Transposable Elements; D005798:Genes, Bacterial; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000472:pathogenicity*; D016324:Sequence Tagged Sites; D014774:Virulence / Q000235:genetics
chemical_list: D004251:DNA Transposable Elements