Oct-2 is required early in T cell-independent B cell activation for G1 progression and for proliferation.
Oct-2, a POU homeodomain protein expressed primarily in B cells, is a powerful transcriptional activator that binds to DNA at sites appropriately placed for major effects on immunoglobulin gene expression. Our examination of B cell development and function in Oct-2 null mice did not support an essential role for Oct-2 early in B cell development. Rather, Oct-2 was required later, when B cells were induced to differentiate to antibody-secreting cells. We show here that Oct-2 is not required for normal immunoglobulin production by mature B lymphocytes. Instead, it is essential for a normal proliferative response to polyclonal mitogens. Responses to signals from activated T cells are unaffected. The requirement for Oct-2 maps to an early activation step in G1, during which B cells make the commitment to progress through the cell cycle and to divide.
Corcoran
L M
LM
Walter and Eliza Hall Institute of Medical Research Post Office, Royal Melbourne Hospital, Victoria, Australia.
Karvelas
M
M
D000818:Animals; D000921:Antibody-Producing Cells / Q000378:metabolism; D000944:Antigens, Differentiation, B-Lymphocyte / Q000096:biosynthesis / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology*; D001853:Bone Marrow; D002454:Cell Differentiation; D004268:DNA-Binding Proteins / Q000502:physiology; D016193:G1 Phase; D007132:Immunoglobulin Isotypes / Q000096:biosynthesis; D016219:Immunotherapy, Adoptive; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D051379:Mice; D016513:Mice, SCID; D016254:Mutagenesis, Insertional; D050813:Octamer Transcription Factor-2; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*
D000944:Antigens, Differentiation, B-Lymphocyte; D004268:DNA-Binding Proteins; D007132:Immunoglobulin Isotypes; D050813:Octamer Transcription Factor-2; C489206:Pou2f2 protein, mouse; D014157:Transcription Factors