Mononuclear cells isolated from fibrotic skin lesions in avian scleroderma constitutively produce fibroblast-activating cytokines and immunoglobulin M.
University of California, Davis line 200 and 206 chickens develop an inherited autoimmune fibrotic disease associated with autoantibodies and other features similar to human scleroderma. Early in life, line 200/206 chickens develop mononuclear cell (MNC) infiltrates in the skin, followed by severe dermal fibrosis and vascular occlusions. Cultured fibroblasts derived from fibrotic skin lesions of line 200/206 chickens display an activated phenotype producing elevated quantities of collagen and glycosaminoglycan (GAG) compared to fibroblasts derived from normal chicken skin. To demonstrate a link between skin mononuclear cell infiltration and fibroblast activation, we cultured MNC isolated from developing fibrotic skin lesions, normal appearing skin, and peripheral blood of line 206 chickens for 24-72 h. Subsequent assay of MNC culture supernatants for effect on the collagen and GAG production of normal chicken skin fibroblasts demonstrated that only fibrotic lesion MNC supernatants contained significant collagen and GAG synthesis stimulatory activity. Both stimulatory activities were constitutively produced, undialyzable, heat and protease sensitive, and coeluted on gel filtration with a M(r) of 24-32 kD. Gel filtration also revealed that fibrotic lesion MNC secrete a protein > 250 kD, which we have identified as immunoglobulin (Ig) M by Western blot analysis. These results demonstrate that skin infiltrating MNC secrete both fibroblast-activating cytokines and IgM and thus they likely play an important role as effector cells in the development of dermal fibrosis and autoantibodies in this avian model of scleroderma.
Duncan
M R
MR
Department of Internal Medicine, University of California Davis, School of Medicine, USA.
Berman
B
B
Van de Water
J
J
Boyd
R L
RL
Wick
G
G
Gershwin
M E
ME
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D002850:Chromatography, Gel; D016207:Cytokines / Q000096:biosynthesis*; D005347:Fibroblasts / Q000473:pathology; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D012595:Scleroderma, Systemic / Q000378:metabolism* / Q000473:pathology; D012867:Skin / Q000473:pathology*
D016207:Cytokines; D007075:Immunoglobulin M