title: Structure/function analysis of human interleukin 5 and its receptor.
abstract: We have performed a detailed structure-function analysis of human interleukin 5 (hIL5) and its receptor. By testing a hIL5 mutant panel in a solid phase binding assay and a proliferation assay using hIL5 dependent cell-lines, areas on hIL5 involved in either the receptor alpha-subunit interaction or in receptor activation were identified. Epitope mapping data of a neutralizing and a non-neutralizing monoclonal antibody were in agreement with the mutant analysis. hIL5 binding areas on the IL5R alpha-subunit were identified by interspecies chimaera analysis. Finally, hIL5 mutants with reduced receptor activation potential have antagonistic properties.
authors.affiliation: Roche Research Gent, Belgium.
authors.forename: J
authors.initials: J
authors.lastname: Tavernier
authors.forename: S
authors.initials: S
authors.lastname: Cornelis
authors.forename: R
authors.initials: R
authors.lastname: Devos
authors.forename: Y
authors.initials: Y
authors.lastname: Guisez
authors.forename: G
authors.initials: G
authors.lastname: Plaetinck
authors.forename: J
authors.initials: J
authors.lastname: Van der Heyden
mesh_terms: D000818:Animals; D006801:Humans; D015848:Interleukin-5 / Q000737:chemistry / Q000502:physiology*; D051379:Mice; D015394:Molecular Structure; D009154:Mutation; D010446:Peptide Fragments / Q000737:chemistry / Q000502:physiology; D018123:Receptors, Interleukin / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D053649:Receptors, Interleukin-5; D013329:Structure-Activity Relationship
chemical_list: D015848:Interleukin-5; D010446:Peptide Fragments; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5