Indirect gene diagnoses for complex (multifactorial) diseases--a review.
The analysis of multifactorial diseases requires the efficient investigation of large numbers of (gene) loci and patient (family) samples. Since simple repetitive DNA markers are dispersed all over the chromosomes, molecular techniques employing these tools render most conventional screening procedures obsolete. Examples of tumors, autoimmune diseases and infections are presented to validate concepts of indirect gene diagnoses via simple, tandemly arranged, repetitive DNA sequences. The salient advantages of microsatellite technologies vs. those of multilocus DNA fingerprinting are weighed.
Epplen
J T
JT
Ruhr University, Bochum, Germany.
Buitkamp
J
J
Bocker
T
T
Epplen
C
C
D000818:Animals; D001327:Autoimmune Diseases / Q000175:diagnosis / Q000235:genetics*; D016172:DNA Fingerprinting / Q000379:methods; D005819:Genetic Markers; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics*; D009838:Oligodeoxyribonucleotides; D010029:Ostertagiasis / Q000175:diagnosis / Q000235:genetics* / Q000662:veterinary; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000235:genetics
D005819:Genetic Markers; D009838:Oligodeoxyribonucleotides