title: Diabetes affects alpha-crystallin chaperone function.
abstract: In vitro glycation was previously shown to influence alpha-crystallin chaperone function. In the present study we show that this function is compromised in diabetes. The alpha H, alpha L and the total alpha fractions were isolated by gel permeation chromatography from the water-soluble protein of streptozotocin-diabetic as well as age-matched normal rats. Based on the beta L-crystallin thermal denaturation assay the chaperone function was significantly decreased in the diabetic rats.
authors.affiliation: Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta 30912-2100, USA.
authors.forename: M
authors.initials: M
authors.lastname: Cherian
authors.forename: E C
authors.initials: EC
authors.lastname: Abraham
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D018833:Chaperonins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002850:Chromatography, Gel; D003459:Crystallins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D006031:Glycosylation; D011489:Protein Denaturation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D003459:Crystallins; D018833:Chaperonins