title: Nucleotide sequence of the region between crr and cysM in Salmonella typhimurium: five novel ORFs including one encoding a putative transcriptional regulator of the phosphotransferase system.
abstract: A 4471 bp region between crr and cysM on the Salmonella typhimurium chromosome (49.5 min) has been sequenced. Five ORFs were found within this region, one of which is likely to be the putative regulatory gene, ptsJ, that corresponds in map position to a gene which when mutated allows expression of a cryptic Enzyme I of the phosphotransferase system. The deduced amino acid sequence of the encoded protein is similar to those of several open reading frames (ORFs) including ORFT2 of Rhodobacter spheroides with which it is 28% identical throughout most of its length (comparison score of 21 S.D.). PtsJ exhibits a putative, N-terminal, helix-turn-helix, DNA binding domain that is similar in sequence to those in members of the GntR family of transcriptional regulators. Analyses of the sequences of the ORFs encoded within this region are presented.
authors.affiliation: Department of Biology, University of California at San Diego, La Jolla 92093-0116, USA.
authors.forename: F
authors.initials: F
authors.lastname: Titgemeyer
authors.forename: J
authors.initials: J
authors.lastname: Reizer
authors.forename: A
authors.initials: A
authors.lastname: Reizer
authors.forename: J
authors.initials: J
authors.lastname: Tang
authors.forename: T R
authors.initials: TR
authors.lastname: Parr
authors.forename: M H
authors.initials: MH
authors.lastname: Saier
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005809:Genes, Regulator; D018257:Helix-Loop-Helix Motifs; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics* / Q000378:metabolism; D017852:Phosphotransferases (Nitrogenous Group Acceptor) / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics; D015183:Restriction Mapping; D012242:Rhodobacter sphaeroides / Q000235:genetics; D012486:Salmonella typhimurium / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C081598:GntR protein, E coli; D012097:Repressor Proteins; D014157:Transcription Factors; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D017852:Phosphotransferases (Nitrogenous Group Acceptor); C022360:phosphoenolpyruvate-protein phosphotransferase