Comparison of a direct antigen enzyme immunoassay, Herpchek, with cell culture for detection of herpes simplex virus from clinical specimens.
Direct enzyme immunoassay (Herpchek) was compared with culture on 21,522 specimens mainly from asymptomatic women for herpes simplex virus detection. Sensitivity and specificity were 73.8 and 97.7%, respectively. The 33% detection rate by enzyme immunoassay in 5 h increased to 43% when the enzyme immunoassay was combined with culture. Herpchek alone was not sensitive enough, but in combination with culture, maximum detection was achieved.
Verano
L
L
Diagnostic Virology Laboratory, Corning Clinical Laboratory, Teterboro, New Jersey 07608, USA.
Michalski
F J
FJ
D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005260:Female; D006558:Herpes Genitalis / Q000150:complications / Q000175:diagnosis / Q000821:virology; D006561:Herpes Simplex / Q000150:complications / Q000175:diagnosis / Q000821:virology; D018259:Herpesvirus 1, Human / Q000276:immunology / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data; D008297:Male; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis / Q000821:virology; D012680:Sensitivity and Specificity; D018139:Simplexvirus / Q000276:immunology / Q000302:isolation & purification*; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data; D014776:Virus Cultivation / Q000379:methods / Q000706:statistics & numerical data
D000956:Antigens, Viral