title: Listeria monocytogenes infection increases neutrophil adhesion and damage to a murine hepatocyte cell line in vitro.
abstract: Several studies have reported that Listeria monocytogenes multiples within hepatocytes and that inflammatory neutrophils inhibit this intracellular growth in vivo. In the present study, we used a murine embryonic hepatocyte cell line (ATCC TIB73) as an in vitro model to investigate neutrophil-hepatocyte interactions. Murine peritoneal exudate neutrophils adhered more readily to L. monocytogenes-infected hepatocyte monolayers than to uninfected monolayers or monolayers infected with actA- and hly- mutants of L. monocytogenes. L. monocytogenes-infected TIB73 cells increased their surface expression of ICAM-1 as compared with uninfected TIB73 cells. Neutrophil adherence and oxidative stress to TIB73 cells were reduced by pre-incubating the hepatocyte monolayers with anti-ICAM-1 monoclonal antibody and diminished further by pre-incubating the peritoneal exudate neutrophils with an anti-CR3 monoclonal antibody.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Wisconsin-Madison 53706, USA.
authors.forename: N M
authors.initials: NM
authors.lastname: Boury
authors.forename: C J
authors.initials: CJ
authors.lastname: Czuprynski
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002448:Cell Adhesion / Q000276:immunology*; D002460:Cell Line; D007166:Immunosuppressive Agents / Q000494:pharmacology; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D008089:Listeria monocytogenes / Q000235:genetics / Q000276:immunology; D008088:Listeriosis / Q000276:immunology*; D008099:Liver / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D016177:Macrophage-1 Antigen / Q000276:immunology; D051379:Mice; D009504:Neutrophils / Q000276:immunology / Q000382:microbiology*; D018384:Oxidative Stress / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D007166:Immunosuppressive Agents; D016177:Macrophage-1 Antigen; D018799:Intercellular Adhesion Molecule-1