Chondroitin sulfate is a major proteoglycan constituent of human cameral mucous gel.
The presence of chondroitin- and heparan sulfate-containing proteoglycans and type IV collagen was searched for in human cameral mucous gel (CMG). Monoclonal antibodies specific for human heparan sulfate, chondroitin sulfate and collagen IV were used in ultrathin sections of Lowicryl-embedded human CMG. Only chondroitin sulfate is revealed with this method. Heparan sulfate and collagen IV were not elicited by the monoclonal antibodies. We conclude that chondroitin sulfate is a major component of the proteoglycans of human CMG, where it may contribute to the colloidosmotic properties of the gel and play a role in the outflow of aqueous humor.
Carreras
F J
FJ
Department of Surgery (Ophthalmology), Faculty of Medicine, University of Granada, Spain.
Porcel
D
D
D000867:Anterior Chamber / Q000737:chemistry*; D000911:Antibodies, Monoclonal; D002809:Chondroitin Sulfates / Q000032:analysis*; D003094:Collagen / Q000032:analysis; D005782:Gels; D006497:Heparan Sulfate / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D008875:Middle Aged; D009093:Mucus / Q000737:chemistry*; D011509:Proteoglycans / Q000032:analysis*
D000911:Antibodies, Monoclonal; D002809:Chondroitin Sulfates; D003094:Collagen; D005782:Gels; D006497:Heparan Sulfate; D011509:Proteoglycans