title: Confocal microscopy analysis of NPY and TH immunoreactivities in the hypothalamo-hypophysial system of the frog.
abstract: Co-expression of tyrosine hydroxylase (TH) and neuropeptide Y (NPY) in local circuits innervating the hypothalamo-pituitary complex of the green frog, Rana ridibunda, was investigated using simultaneous double immunohistochemical technique, aided by dual-channel confocal laser scanning microscopy. NPY and TH immunoreactivities were observed co-occurring within a discrete neuronal population located in the suprachiasmatic region. In other hypothalamic areas, NPY-immunoreactive (IR) perikarya were generally codistributed, but distinct from TH-IR cells. In the adenohypophysial pars intermedia, the overlap between the two markers was partial, demonstrating the existence of multiple neuronal sources for the inputs to the gland.
authors.affiliation: Department of Animal Biology, University of Torino, Italy.
authors.forename: A A
authors.initials: AA
authors.lastname: Battaglia
authors.forename: M
authors.initials: M
authors.lastname: Feuilloley
authors.forename: B
authors.initials: B
authors.lastname: Mulatero
authors.forename: M
authors.initials: M
authors.lastname: Beltramo
authors.forename: J
authors.initials: J
authors.lastname: Thibault
authors.forename: M F
authors.initials: MF
authors.lastname: Franzoni
authors.forename: A
authors.initials: A
authors.lastname: Calas
authors.forename: H
authors.initials: H
authors.lastname: Vaudry
authors.forename: A
authors.initials: A
authors.lastname: Fasolo
mesh_terms: D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D007030:Hypothalamo-Hypophyseal System / Q000737:chemistry* / Q000166:cytology; D008297:Male; D018613:Microscopy, Confocal; D009412:Nerve Fibers / Q000737:chemistry; D009474:Neurons / Q000737:chemistry; D009478:Neuropeptide Y / Q000032:analysis*; D011895:Rana ridibunda; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
chemical_list: D009478:Neuropeptide Y; D014446:Tyrosine 3-Monooxygenase