Reduced capacity of antibodies from patients infected with human immunodeficiency virus type 1 (HIV-1) group O to neutralize primary isolates of HIV-1 group M viruses.
Neutralizing antibody patterns in sera of persons infected with human immunodeficiency virus type 1 (HIV-1) groups M and O to their homologous and heterologous primary isolates were determined in a peripheral blood mononuclear cell-based neutralization assay and correlated with their ability to bind to V3 loop synthetic peptides. Most HIV-1 group M sera (9/16) neutralized HIV-1 group O viruses, whereas fewer group O sera (3/13) only weakly neutralized HIV-1 group M viruses. Group M sera neutralizing HIV-1 group O viruses neutralized other HIV-1 group M viruses with titers of 1:10-1:1280. V3 loop binding capacity of sera did not reflect their neutralizing capacity of the homologous isolate. Despite the reduced neutralizing capacity of group O-infected patients' sera to group M viruses, some group M-infected patients' sera neutralized both HIV-1 group M and O isolates, suggesting that they share some conserved neutralizing epitopes.
Nyambi
P N
PN
Institute of Tropical Medicine, Antwerp, Belgium.
Nkengasong
J
J
Peeters
M
M
Simon
F
F
Eberle
J
J
Janssens
W
W
Fransen
K
K
Willems
B
B
Vereecken
K
K
Heyndrickx
L
L
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood / Q000276:immunology*; D000595:Amino Acid Sequence; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology*; D015686:Gene Products, env / Q000235:genetics / Q000276:immunology*; D015752:Genes, env; D015750:Genes, gag; D015483:HIV Antibodies / Q000097:blood / Q000276:immunology*; D015497:HIV-1 / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D008214:Lymphocytes / Q000276:immunology* / Q000821:virology; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D010802:Phylogeny
D000939:Epitopes; D015686:Gene Products, env; D015483:HIV Antibodies; D010446:Peptide Fragments