1H NMR approach to observe tissue oxygenation with the signals of myoglobin.
The myoglobin technique measures oxygen tension in myocytes. It relies on a quantitative measurement of the Val E11 and His F8 signals and an accurate value for the [O2]50 for Mb. Even though the Mb oxygen affinity in the cell is in question, the NMR results still reflect the degree of Mb oxygen saturation. Although magnetic resonance has established a variety of strategies to measure tissue oxygenation, the Mb approach is the most direct and will lead to a better understanding of oxygen's role in regulating cellular activity.
Jue
T
T
Biological Chemistry Department, University of California Davis 95616-8635, USA.
Kreutzer
U
U
Chung
Y
Y
D000818:Animals; D006639:Histidine / Q000737:chemistry; D006859:Hydrogen; D066298:In Vitro Techniques; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D009206:Myocardium / Q000378:metabolism; D009211:Myoglobin / Q000737:chemistry / Q000378:metabolism*; D010100:Oxygen / Q000032:analysis / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism; D011485:Protein Binding; D014018:Tissue Distribution; D014633:Valine / Q000737:chemistry
D009211:Myoglobin; D010725:Phosphocreatine; D006639:Histidine; D006859:Hydrogen; D014633:Valine; D010100:Oxygen