title: Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene.
abstract: An efficient restriction barrier for methylated DNA in the actinomycete Amycolatopsis methanolica could be avoided by using a nonmethylating Escherichia coli strain for DNA isolations. The A. methanolica prephenate dehydratase gene was cloned from a gene bank in a pMEA300-derived shuttle vector in E. coli and characterized.
authors.affiliation: Department of Microbiology, University of Groningen, Haren, The Netherlands.
authors.forename: J W
authors.initials: JW
authors.lastname: Vrijbloed
authors.forename: J
authors.initials: J
authors.lastname: van Hylckama Vlieg
authors.forename: N M
authors.initials: NM
authors.lastname: van der Put
authors.forename: G I
authors.initials: GI
authors.lastname: Hessels
authors.forename: L
authors.initials: L
authors.lastname: Dijkhuizen
mesh_terms: D039903:Actinobacteria / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D011302:Prephenate Dehydratase / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014169:Transformation, Bacterial
chemical_list: D011302:Prephenate Dehydratase