Regulation of the multiple antibiotic resistance (mar) regulon by marORA sequences in Escherichia coli.
The mar operon and adjacent sequences were subcloned on a low-copy-number plasmid to identify essential regulatory elements. A 1.1-kbp fragment containing 133 bp of the operator-promoter region (marO), the full marRA gene sequences, and only 10 of 72 marB codons provided a dela mar strain with normal repressibility and inducibility and the ability to beget mar constitutive mutants.
Martin
R G
RG
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892-0560, USA.
Nyantakyi
P S
PS
Rosner
J L
JL
D001426:Bacterial Proteins / Q000235:genetics; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins; D004352:Drug Resistance, Microbial / Q000235:genetics*; D018432:Drug Resistance, Multiple / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D016296:Mutagenesis; D009875:Operator Regions, Genetic / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D018085:Regulon / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C080002:MarA protein, E coli; C080004:MarR protein, E coli; D012097:Repressor Proteins; D014157:Transcription Factors