Human gene for beta-microseminoprotein: its promoter structure and chromosomal localization.
The gene for human beta-microseminoprotein (MSP, beta-inhibin, prostatic secretory protein94, or PSP94) was isolated. The nucleotide sequence of its upstream region (2,860 bp), exon 1 (35 bp), and a part of the following intron (218 bp) was determined. Transient transfection analysis on MSP-expressing (PC-3) and -nonexpressing (HepG2) cells using luciferase reporter plasmids suggested that the region from -2738 to -276 is not essential for the basal promoter activity, and that the regions from -275 to -207 and from -186 to -128 function in a cell-specific manner. The chromosome locus of the gene (MSMB) was determined through application of PCR to the DNAs of rodent-human somatic cell hybrids and also by the fluorescence in situ hybridization technique to be region q11.2 of chromosome 10.
Ochiai
Y
Y
Department of Medical Biochemistry, Shiga University of Medical Science.
Inazawa
J
J
Ueyama
H
H
Ohkubo
I
I
D000818:Animals; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D017931:DNA Primers; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008113:Liver Neoplasms; D008297:Male; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms; D029584:Prostatic Secretory Proteins; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D029589:Seminal Plasma Proteins; D016385:TATA Box; D013735:Testicular Hormones / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D017931:DNA Primers; D029584:Prostatic Secretory Proteins; D011506:Proteins; D011994:Recombinant Proteins; D029589:Seminal Plasma Proteins; D013735:Testicular Hormones; C043076:beta-microseminoprotein; D015500:Chloramphenicol O-Acetyltransferase