Ultraviolet B and melanocyte-stimulating hormone (MSH) stimulate mRNA production for alpha MSH receptors and proopiomelanocortin-derived peptides in mouse melanoma cells and transformed keratinocytes.
Cell lines of cutaneous origin, namely melanocytes and keratinocytes, were previously demonstrated to exhibit functional melanocyte-stimulating hormone (MSH) receptors that are up-regulated by ultraviolet (UV) radiation and by MSH itself. In this study, it is demonstrated that UVB irradiation, exposure to MSH, or exposure to N6,O2-dibutyryl cyclic adenosine monophosphate stimulates production of mRNAs for both alpha MSH receptors and proopiomelanocortin in cultured mouse Cloudman S91 melanoma cells, and that UVB stimulates production and release of MSH and adrenocorticotropin peptides in both melanoma cells and transformed PAM 212 mouse keratinocytes. The results add support to the hypothesis that the effects of UVB on cutaneous melanogenesis are mediated through a series of coordinated events in which MSH receptors and proopiomelanocortin-derived peptides play a central role.
Chakraborty
A
A
Department of Dermatology, Yale School of Medicine, New Haven, Connecticut 06520, USA.
Slominski
A
A
Ermak
G
G
Hwang
J
J
Pawelek
J
J
D000818:Animals; D002461:Cell Line, Transformed; D015603:Keratinocytes / Q000166:cytology* / Q000378:metabolism; D009074:Melanocyte-Stimulating Hormones / Q000494:pharmacology*; D008546:Melanoma, Experimental; D051379:Mice; D011333:Pro-Opiomelanocortin / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects*; D012333:RNA, Messenger / Q000378:metabolism*; D011979:Receptors, Pituitary Hormone / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects*; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays; D000521:alpha-MSH / Q000378:metabolism
D012333:RNA, Messenger; D011979:Receptors, Pituitary Hormone; D000521:alpha-MSH; D011333:Pro-Opiomelanocortin; C029446:MSH receptor; D009074:Melanocyte-Stimulating Hormones