PCR detection of the lactocin S structural gene in bacteriocin-producing lactobacilli from meat.
The lactocin S structural gene (lasA) in seven bacteriocinogenic lactobacilli isolated from fermented sausages was studied. Two degenerate primers were synthesized to amplify a 75-bp fragment of the gene. Three strains amplified the fragment from their plasmid DNA, and hybridization analysis confirmed these results.
Rodríguez
J M
JM
Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Cintas
L M
LM
Casaus
P
P
Suárez
A
A
Hernández
P E
PE
D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000235:genetics*; D001483:Base Sequence; D005516:Food Microbiology; D005798:Genes, Bacterial; D007778:Lactobacillus / Q000235:genetics*; D008460:Meat; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D001430:Bacteriocins