Prednisolone excitation of medial vestibular nucleus neurons in cats.
An electrophysiological study was performed to determine whether prednisolone hydrochloride directly influenced neuronal activities of the medial vestibular nucleus (MVN) in alpha-chloralose-anesthetized cats. Single neuronal activities of MVN were recorded extracellularly with a glass-insulated silver wire microelectrode attached along a seven-barreled micropipette. Each barrel was filled with prednisolone, glutamate, glutamic acid diethylester (GDEE) or CoCl2. Except for prednisolone, which was administered both intravenously and microiontophoretically, other chemicals were applied microiontophoretically to the immediate vicinity of the target neurons. These MVN neurons were classified as type I and II neurons according to their responses to horizontal and sinusoidal rotations. Intravenous prednisolone (up to 5 mg/kg) enhanced spontaneous and rotation-induced neuronal firings of both type I and II neurons in a dose-dependent manner. In a similar tendency, microiontophoretically applied prednisolone (50-200 nA) dose-dependently increased spontaneous and rotation-induced firings of both type I and II neurons. Microiontophoretic GDEE, a non-selective glutamate receptor antagonist, inhibited glutamate- and rotation-induced neuronal discharges without affecting prednisolone-induced increases in neuronal responses of MVN. In addition, iontophoretically applied CoCl2, a Ca2+ channel blocker, did not affect prednisolone-, glutamate- and rotation-induced neuronal findings of MVN. These results suggest that prednisolone induces excitation of type I and II neurons, probably by acting directly on the membrane of MVN neurons. Thus, glucocorticoids such as prednisolone may be effective for the treatment of vertigo resulting from hypofunction of vestibular nucleus neurons.
Yamanaka
T
T
Department of Oto-Rhino-Laryngology, Nara Medical University, Japan.
Amano
T
T
Sasa
M
M
Matsunaga
T
T
D000818:Animals; D002415:Cats; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D004986:Ether / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D007267:Injections; D007478:Iontophoresis; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D011239:Prednisolone / Q000494:pharmacology*; D012399:Rotation; D014726:Vestibular Nuclei / Q000187:drug effects* / Q000502:physiology
D004986:Ether; D018698:Glutamic Acid; D011239:Prednisolone