Inhibitory effects of coffee on transplacental genotoxicity in mice.
Experiments were carried out to ascertain whether or not coffee can modulate the genotoxicity of transplacentally active genotoxins/carcinogens. Coffee was orally administered to Swiss albino mice (gestation, 15-16 days), 90 min before exposure to cyclophosphamide (CPH), N-nitrosodiethylamine (DEN), N-nitroso-N-ethylurea (ENU) and mitomycin C (MMC). At the end of the treatment, the induction of micronucleated polychromatic erythrocytes (MnPCEs) was evaluated in the fetal liver (FL), fetal blood (FB) and maternal bone marrow (MBM). The results of this transplacental micronucleus test showed a consistent trend which suggests that the administration of coffee instead of water (control) can significantly inhibit the genotoxic effects of CPH, DEN, ENU and MMC in the FL and FB. When the fetal cells were evaluated either 22 and 28 h after CPH treatment, or 24 and 48 h after MMC treatment, there was no evidence for a significant interaction between the sampling time and the inhibitory effect of coffee (two-factor ANOVA). However, a significant interaction was observed between sampling time and the inhibitory effects of coffee when the fetal cells were sampled 24 and 40 h after DEN treatment (two-factor ANOVA). Coffee was also effective in significantly inhibiting the genotoxicity of CPH, ENU and MMC in the MBM. The differential response of fetal and maternal target cells was evident from this study.
Abraham
S K
SK
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
D000704:Analysis of Variance; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D003069:Coffee; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors / Q000378:metabolism; D004052:Diethylnitrosamine / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects; D005038:Ethylnitrosourea / Q000378:metabolism; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008431:Maternal-Fetal Exchange / Q000187:drug effects*; D051379:Mice; D015162:Micronucleus Tests; D016685:Mitomycin / Q000037:antagonists & inhibitors / Q000378:metabolism; D016012:Poisson Distribution; D011247:Pregnancy
D016587:Antimutagenic Agents; D003069:Coffee; D004052:Diethylnitrosamine; D016685:Mitomycin; D003520:Cyclophosphamide; D005038:Ethylnitrosourea