title: Genetic relationships among strains of Xylella fastidiosa from RAPD-PCR data.
abstract: Genetic relationships among 11 Xylella fastidiosa strains isolated from mulberry, almond, ragweed, grape, plum, elm, and citrus were determined by random amplified polymorphic DNA (RAPD). Twenty-two 10-base primers amplified a total of 77 discrete polymorphic bands. Phenetic analysis based on a similarity matrix corresponded well with previous reports on X. fastidiosa RFLP-based similarity relationships, indicating that RAPD-PCR amplification products can be used as a reliable indicator of genetic distance in X. fastidiosa. Cladistic analysis suggests the existence of five groups of X. fastidiosa: the citrus group, the plum-elm group, the grape-ragweed group, the almond group, and the mulberry group.
authors.affiliation: United States Department of Agriculture, Beltsville Agricultural Research Center, MD 20705, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Pooler
authors.forename: J S
authors.initials: JS
authors.lastname: Hartung
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
chemical_list: D017931:DNA Primers