title: Inhibition of S-fimbria-mediated adhesion to human ileostomy glycoproteins by a protein isolated from bovine colostrum.
abstract: The aim of this study was to isolate and purify the component in bovine colostrum which is responsible for the inhibition of S-fimbria-mediated adhesion of Escherichia coli. Whey from defatted colostrum was fractionated by ultrafiltration, and the < 100K, < 30K, and < 10K fractions and the colostral whey were tested for inhibition of in vitro adhesion of radiolabelled S-fimbria-bearing E. coli to human ileostomy glycoproteins, which provide a model for human intestinal mucus. The inhibiting compound was purified from a dialyzed < 30K fraction with an anion exchange column which was eluted with a NaCl gradient (0 to 1.0 M). The compound was found to be a heat-resistant but pepsin-sensitive protein with an Mr of approximately 18,000 and an isoelectric point of approximately 5.75. The protein appears to block receptor sites for S-fimbriae on ileostomy glycoproteins, with steric hindrance being the most likely mechanism. Analysis of the amino acid sequence of the amino terminus of the 18K protein showed similarity with the sequence of beta-lactoglobulin.
authors.affiliation: Department of General and Marine Microbiology, Göteborg University, Sweden.
authors.forename: A C
authors.initials: AC
authors.lastname: Ouwehand
authors.forename: P L
authors.initials: PL
authors.lastname: Conway
authors.forename: S J
authors.initials: SJ
authors.lastname: Salminen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D002417:Cattle; D003126:Colostrum / Q000737:chemistry*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007081:Ileostomy; D008969:Molecular Sequence Data; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D006023:Glycoproteins; D011506:Proteins