[Effect of various culture media on growth behavior of human squamous epithelial carcinoma cells of the upper aerodigestive tract].
Sera containing media are frequently used for cultivation of epithelial cells, but the influence of serum on analytic methods can be significant. For this reason the development of serum-free media is of great significance. We investigated the influence of serum-free medium on growth characteristics of human squamous cell carcinoma (SCC) cells of the upper aerodigestive tract and compared these in serum-containing media, using cloned SCC cell lines HLaC-78 and HCFMK-1. Three serum-containing culture media were used: MEM-Instamed, RPMI-1640 and MCDB-153, each containing 10% fetal calf serum. Serum-free culture medium was MCDB-153 supplemented with bovine hypophysis extract, insulin and ethanolamine. Results showed that supplement-enriched, serum-free medium was suitable for culturing SCC cells. When compared to serum-containing media, cell growth of the SCC cell lines was significantly higher in the serum-free medium.
Görögh
T
T
Klinik für HNO-Heilkunde, Universität Kiel.
Lippert
B M
BM
Saffran
S
S
Heissenberg
M
M
Werner
J A
JA
D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002417:Cattle; D002455:Cell Division / Q000502:physiology*; D002460:Cell Line; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D006133:Growth Substances / Q000502:physiology*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D008855:Microscopy, Electron, Scanning; D009062:Mouth Neoplasms / Q000473:pathology*; D012710:Serum Albumin, Bovine; D014407:Tumor Cells, Cultured / Q000473:pathology*
D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D006133:Growth Substances; D012710:Serum Albumin, Bovine