ID--a dominant negative regulator of skeletal muscle differentiation--is not involved in maturation or differentiation of vascular smooth muscle cells.
Rat aortic vascular smooth muscle cells (VSMCs) expressing the MyoD gene formed myotubes at a low frequency in the presence of serum but at a high frequency in the absence of serum. Expression of an antisense Id gene increased myotube formation in the presence of serum indicating that a reduction in Id levels is a major mechanism by which serum withdrawal promotes myotube formation. The role of Id in the development of VSMCs was investigated by expressing an antisense Id gene in neonatal VSMCs. No evidence was found for the conversion of neonatal VSMCs to adult VSMCs in the presence of the antisense Id gene. Similarly reduction in Id by serum withdrawal also failed to cause conversion of the neonatal VSMCs to the adult phenotype. These data suggest that the maturation of neonatal smooth muscle cells is not controlled by a VSMC homologue of the skeletal muscle basic-helix-loop-helix proteins.
Kemp
P R
PR
Department of Biochemistry, Cambridge University, UK.
Metcalfe
J C
JC
Grainger
D J
DJ
D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D001769:Blood; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000502:physiology*; D018257:Helix-Loop-Helix Motifs; D051798:Inhibitor of Differentiation Protein 1; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects / Q000196:embryology; D017570:MyoD Protein / Q000235:genetics / Q000502:physiology; D051381:Rats; D012097:Repressor Proteins; D014157:Transcription Factors; D014162:Transfection
D000081:Acetamides; D004268:DNA-Binding Proteins; C497454:ID1 protein, rat; D051798:Inhibitor of Differentiation Protein 1; D017570:MyoD Protein; D012097:Repressor Proteins; D014157:Transcription Factors; C014026:hexamethylene bisacetamide