Pseudorabies virus early protein 0 transactivates the viral gene promoters.
Pseudorabies virus (PRV) early protein 0 (EP0) contains the RING finger domain with homology to the immediate-early (IE) protein ICP0 of herpes simplex virus type 1 (HSV-1). EP0 was detected by indirect immunofluorescence in the nuclei of the cells transfected with EP0 expression plasmid as is the case in cells infected with PRV. In transient expression assays, EP0 transactivated the PRV IE, thymidine kinase (TK) and glycoprotein X (gX) promoters, indicating that EP0, like ICP0 of HSV-1, is a transactivating protein.
Watanabe
S
S
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Ono
E
E
Shimizu
Y
Y
Kida
H
H
D000818:Animals; D000914:Antibodies, Viral; D002460:Cell Line; D002522:Chlorocebus aethiops; D011558:Herpesvirus 1, Suid / Q000235:genetics*; D017874:Immediate-Early Proteins / Q000235:genetics; D011401:Promoter Regions, Genetic; D013937:Thymidine Kinase / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D015533:Transcriptional Activation; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology / Q000502:physiology*
D000914:Antibodies, Viral; D017874:Immediate-Early Proteins; D015534:Trans-Activators; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C042966:pseudorabies virus glycoproteins; C070914:early protein O, Pseudorabies virus; D013937:Thymidine Kinase