title: A GCN-like response in Candida albicans.
abstract: The control of amino acid and purine biosynthesis in the yeast Saccharomyces cerevisiae is mediated by the transcriptional activator GCN4. We previously identified the presence of two putative GCN4 responsive elements (GCREs) in the promoter sequence of the Candida albicans ARO3 gene, which encodes an enzyme in the aromatic amino acid pathway. We now show that amino acid deprivation results in a dramatic rise in the steady-state level of ARO3-specific mRNA, indicative of a GCN-like pathway in C. albicans.
authors.affiliation: Department of Molecular Biology, Lehigh University, Bethlehem, PA 18015, USA.
authors.forename: S A
authors.initials: SA
authors.lastname: Pereira
authors.forename: G P
authors.initials: GP
authors.lastname: Livi
mesh_terms: D000596:Amino Acids / Q000172:deficiency; D000640:Amitrole / Q000494:pharmacology; D002176:Candida albicans / Q000166:cytology / Q000235:genetics* / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000378:metabolism*; D015870:Gene Expression / Q000502:physiology; D011494:Protein Kinases / Q000378:metabolism*; D012331:RNA, Fungal / Q000032:analysis; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology
chemical_list: D000596:Amino Acids; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D011494:Protein Kinases; D000640:Amitrole