title: DNA insertion system for complex yeast shuttle vectors.
abstract: A DNA insertion system, termed marked homologous recombination, was devised for the construction of complex yeast shuttle plasmids. This system, which is efficient, rapid and easy to use, should contribute to our understanding of gene-gene interactions in yeast cells.
authors.affiliation: Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, Gif-sur-Yvette, France.
authors.forename: J
authors.initials: J
authors.lastname: Daniel
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005819:Genetic Markers; D005822:Genetic Vectors; D010957:Plasmids / Q000235:genetics; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014169:Transformation, Bacterial; D014170:Transformation, Genetic
chemical_list: D005819:Genetic Markers