Physiological effect of glucagon in human isolated adipocytes.
In human isolated adipocytes, glucagon induces a dose dependent increment of the glycerol release, which is already observed at physiological concentrations of the hormone. Furthermore, glucagon at 10(-8) M, significantly stimulates the adenylate cyclase activity in both non-solubilized and solubilized fat plasma membranes, and at already 10(-11) M, a significant increment of the adipocyte cAMP content is observed. These data support previous in vivo positive results indicating that glucagon plays a role in human fat metabolism.
Perea
A
A
Department of Metabolism, Nutrition and Hormones, Fundación Jiménez Díaz, Madrid, Spain.
Clemente
F
F
Martinell
J
J
Villanueva-Peñacarrillo
M L
ML
Valverde
I
I
D000262:Adenylyl Cyclases / Q000378:metabolism; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005934:Glucagon / Q000494:pharmacology*; D005990:Glycerol / Q000008:administration & dosage / Q000378:metabolism; D006801:Humans; D008066:Lipolysis / Q000187:drug effects; D009638:Norepinephrine / Q000494:pharmacology; D012969:Sodium Fluoride / Q000494:pharmacology; D012995:Solubility
D012969:Sodium Fluoride; D005934:Glucagon; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D005990:Glycerol; D009638:Norepinephrine