title: RMP-7, a bradykinin agonist, increases permeability of blood-ocular barriers in the guinea pig.
abstract: OBJECTIVE
  To determine if the bradykinin agonist, RMP-7, could increase the ocular tissue concentration of agents that normally have limited access across the blood-ocular barriers. The extracellular space marker, sucrose, and the anti-viral drug, ganciclovir, were tested.
  
  
  METHODS
  Using the perfused eye method in guinea pigs, RMP-7 (1 microgram/kg over 5 minutes) or saline were administered intraarterially into the ocular circulation before either radiolabeled sucrose or ganciclovir (0.4 to 0.6 microCi/ml per minute). At time intervals ranging from 0.25 minute to 10 minutes, perfused eyes were removed, and the radioactivity within various compartments was measured using liquid scintillography.
  
  
  RESULTS
  Pretreatment with RMP-7 significantly increased uptake of both sucrose (up to 4.5-fold) and ganciclovir (up to 2-fold) into the guinea pig retina and lens. Smaller and less consistent effects were observed in other eye compartments.
  
  
  CONCLUSIONS
  This report demonstrates that RMP-7 enhances the permeability of blood-ocular barriers, and it provides the first pharmacologic evidence for a means to enhance the concentration of ganciclovir into the retina. Thus, these data support the concept that RMP-7 may prove to be a useful adjunct for enhanced delivery of therapeutics to the eye under conditions in which ocular barriers limit treatment.
authors.affiliation: Alkermes Inc., Los Angeles, California, USA.
authors.forename: P J
authors.initials: PJ
authors.lastname: Elliott
authors.forename: J B
authors.initials: JB
authors.lastname: Mackic
authors.forename: W F
authors.initials: WF
authors.lastname: Graney
authors.forename: R T
authors.initials: RT
authors.lastname: Bartus
authors.forename: B V
authors.initials: BV
authors.lastname: Zlokovic
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000378:metabolism; D001693:Biological Transport, Active; D001769:Blood / Q000378:metabolism*; D018916:Blood-Aqueous Barrier / Q000187:drug effects; D001813:Blood-Retinal Barrier / Q000187:drug effects; D001920:Bradykinin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D002199:Capillary Permeability / Q000187:drug effects*; D003315:Cornea / Q000378:metabolism; D005123:Eye / Q000378:metabolism*; D005260:Female; D015774:Ganciclovir / Q000493:pharmacokinetics; D006168:Guinea Pigs; D007261:Infusions, Intra-Arterial; D008297:Male; D013395:Sucrose / Q000493:pharmacokinetics; D014018:Tissue Distribution; D014822:Vitreous Body / Q000378:metabolism
chemical_list: C089876:RMP 7; D013395:Sucrose; D015774:Ganciclovir; D001920:Bradykinin