Diffusion characteristics of chitosan-entrapped microsomal UDP-glucuronyl transferase gel beads.
Rabbit hepatic microsomal UDP-glucuronyl transferase (EC 2.4.1.17) was immobilized by entrapment in chitosan which is an ionotropic gelation agent and the resulting preparation was used as a biocatalyst for the glucuronidation of 1-naphthol in order to study the drug metabolism in vitro and obtaining artificial liver support (detoxification). In this study, depending on the porous structure of the chitosan/UDPGT gel beads, mainly the diffusion characteristics of 1-naphthol from chitosan gel beads in well stirred solutions were investigated, and the values of the effective diffusion coefficients (Dc) indicate that UDP-glucuronyl transferase in chitosan beads has no additional diffusion barrier. Using the data, the capacity of the chitosan-gel as an immobilization matrix for microsomal UDP-glucuronyl transferase is discussed from the point of view of diffusion characteristics.
Zihnioğlu
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Ege University, Faculty of Science, Department of Biochemistry, Bornova Izmir, Turkey.
Telefoncu
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D000818:Animals; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry; D048271:Chitosan; D004058:Diffusion; D004800:Enzymes, Immobilized; D005782:Gels; D014453:Glucuronosyltransferase / Q000737:chemistry*; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology*; D008863:Microspheres; D009284:Naphthols / Q000737:chemistry / Q000378:metabolism; D011817:Rabbits
D004800:Enzymes, Immobilized; D005782:Gels; D009284:Naphthols; D002686:Chitin; C029350:1-naphthol; D048271:Chitosan; D014453:Glucuronosyltransferase