Detection of XerC and XerD recombinases in gram-negative bacteria of the family Enterobacteriaceae.
XerC and XerD are site-specific recombinases of the lambda integrase family which resolve multimeric replicons to monomers by acting at specific sites such as cer, ckr, nmr, parB, and psi, which are found in plasmids, or at the dif site found in the Escherichia coli chromosome. By using Southern hybridizations to cloned E. coli xerC and xerD genes and a cer-nmr plasmid-based resolution assay, the presence of these genes in several species of Enterobacteriaceae is shown.
Sirois
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Département de Microbiologie et Immunologie, Université de Montréal, Québec, Canada.
Szatmari
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D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D004755:Enterobacteriaceae / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D019426:Integrases; D009693:Nucleic Acid Hybridization; D045522:Recombinases; D011995:Recombination, Genetic; D017385:Sequence Homology; D013045:Species Specificity
D029968:Escherichia coli Proteins; D045522:Recombinases; C473360:XerC protein, E coli; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C473361:XerD protein, E coli; C115006:integron integrase IntI1