title: Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases.
abstract: DNA-activated protein kinase (DNA-PK) is a serine/threonine protein kinase that interacts with a DNA end-binding heterodimeric protein, Ku, and is activated by double-stranded DNA. Genomic clones that contain the DNA-PK gene complement the murine scid defect, indicating that DNA-PK affects double-strand break repair and V(D)J recombination. Here we describe the cDNA sequence of the region that corresponds to about 100 kDa of C-terminal sequence of this large (> p350 kDa) protein. This region contains a kinase domain that has strong homology to phosphatidylinositol kinases.
authors.affiliation: Department of Biological Sciences, St. John's University, Jamaica, NY 11439, USA.
authors.forename: V P
authors.initials: VP
authors.lastname: Poltoratsky
authors.forename: X
authors.initials: X
authors.lastname: Shi
authors.forename: J D
authors.initials: JD
authors.lastname: York
authors.forename: M R
authors.initials: MR
authors.lastname: Lieber
authors.forename: T H
authors.initials: TH
authors.lastname: Carter
mesh_terms: D019870:1-Phosphatidylinositol 4-Kinase; D000595:Amino Acid Sequence; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D051747:DNA-Activated Protein Kinase; D004268:DNA-Binding Proteins; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor); D019870:1-Phosphatidylinositol 4-Kinase; D051747:DNA-Activated Protein Kinase; C497263:PRKDC protein, human; D017346:Protein Serine-Threonine Kinases