title: Genetic variation in Phoca vitulina (the harbour seal) revealed by DNA fingerprinting and RAPDs.
abstract: Genetic variation in two harbour seal (Phoca vitulina) populations from the Dutch Wadden Sea and Scotland was examined by RAPD analysis and DNA fingerprinting. For comparison a population of grey seals (Halichoerus grypus) was studied. The RAPD method revealed a very low number of polymorphic bands. The multilocus DNA fingerprinting technique showed very low levels of variation both within and between populations of the harbour seals. For one probe a significant difference in variation between populations was demonstrated. The estimated average heterozygosity, however, diverged more strongly between the two harbour seal populations because of more frequent occurrence of rare alleles in the Scottish population. Low levels of genetic variation seem to be characteristic for the subspecies Phoca vitulina vitulina, but not for all marine mammals in the North Sea, as the grey seal population showed significantly higher variation.
authors.affiliation: Department of Genetics, University of Groningen, Haren, The Netherlands.
authors.forename: A L
authors.initials: AL
authors.lastname: Kappe
authors.forename: L
authors.initials: L
authors.lastname: van de Zande
authors.forename: E J
authors.initials: EJ
authors.lastname: Vedder
authors.forename: R
authors.initials: R
authors.lastname: Bijlsma
authors.forename: W
authors.initials: W
authors.lastname: van Delden
mesh_terms: D000818:Animals; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods; D017931:DNA Primers; D003718:Denmark; D014644:Genetic Variation; D005828:Genetics, Population; D006579:Heterozygote; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques; D009792:Oceans and Seas; D011110:Polymorphism, Genetic / Q000235:genetics; D012606:Scotland; D046048:Seals, Earless / Q000235:genetics*
chemical_list: D017931:DNA Primers