title: Multiplicity of N-terminal structures of medium-chain alcohol dehydrogenases. Mass-spectrometric analysis of plant, lower vertebrate and higher vertebrate class I, II, and III forms of the enzyme.
abstract: Ten different alcohol dehydrogenases, representing several classes of the enzyme and a wide spread of organisms, were analyzed for patterns of N-terminal structures utilizing a combination of conventional and mass spectrometric peptide analysis. Results show all forms to be N-terminally acetylated and allow comparisons of now 40 such alcohol dehydrogenases covering a large span of forms and origins. Patterns illustrate roles of acetylation in proteins in general, define special importance of the class I N-terminal acetylation, and distinguish separate acetylated structures for all classes, as well as a common alcohol dehydrogenase motif.
authors.affiliation: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Hjelmqvist
authors.forename: M
authors.initials: M
authors.lastname: Hackett
authors.forename: J
authors.initials: J
authors.lastname: Shafqat
authors.forename: O
authors.initials: O
authors.lastname: Danielsson
authors.forename: J
authors.initials: J
authors.lastname: Iida
authors.forename: R C
authors.initials: RC
authors.lastname: Hendrickson
authors.forename: H
authors.initials: H
authors.lastname: Michel
authors.forename: J
authors.initials: J
authors.lastname: Shabanowitz
authors.forename: D F
authors.initials: DF
authors.lastname: Hunt
authors.forename: H
authors.initials: H
authors.lastname: Jörnvall
mesh_terms: D000107:Acetylation; D000426:Alcohol Dehydrogenase / Q000737:chemistry* / Q000145:classification; D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000145:classification; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry; D010944:Plants / Q000201:enzymology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014714:Vertebrates
chemical_list: D007527:Isoenzymes; D010940:Plant Proteins; D000426:Alcohol Dehydrogenase