In vitro differentiation and proliferation in a newly established human rhabdomyosarcoma cell line.
A newly established cell line, designated NRS-1, was derived from an alveolar rhabdomyosarcoma that developed in the left forearm of a 7-year-old girl. The cell line had a t(2; 13) chromosomal translocation. Reverse transcription-polymerase chain reaction demonstrated that 5' PAX3-3' FKHR chimeric transcript was expressed in NRS-1 cells. NRS-1 cells showed myogenic differentiation without any particular stimulus in vitro and exhibited various kinds of muscle markers. All-trans retinoic acid promoted cell differentiation in the myogenic direction. Transforming growth factor-beta (TGF-beta) inhibited myogenic differentiation of those cells and promoted cell proliferation.
Ogose
A
A
Department of Pathology, Niigata University School of Medicine, Japan.
Motoyama
T
T
Hotta
T
T
Watanabe
H
H
D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002461:Cell Line, Transformed; D002648:Child; D005260:Female; D005542:Forearm; D006801:Humans; D007621:Karyotyping; D008969:Molecular Sequence Data; D012208:Rhabdomyosarcoma / Q000235:genetics / Q000473:pathology*; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology*; D015291:Transforming Growth Factors / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D014212:Tretinoin; D015291:Transforming Growth Factors