Identification of malaria-infected dried mosquitoes by biotinylated DNA probe.
Dot-blot hybridization with cloned genomic DNA labeled with photobiotin as a probe is used to detect Plasmodium falciparum sporozoites in dried mosquitoes. The assay is sensitive enough to detect 2 pg Plasmodium parasite DNA, or one infected mosquito in a pool of 40 insects using DNA extraction samples, or one infected mosquito in a pool of 25 insects without DNA extraction. A single mosquito squashed directly on a nitrocellulose filter for the determination of parasites provides a simple method for the detection of sporozoites. The mosquitoes triturated in reduction buffer were efficient for the screening of malaria infection of mosquitoes in a large number of samples. The species specificity, sensitivity, and ease of performance of this assay as well as the stability of the reagents may make it a useful epidemiological tool.
Liu
K Y
KY
Shandong Institute of Parasitic Diseases, People's Republic of China.
Chen
X X
XX
Huing
B C
BC
Cheng
Y L
YL
D000818:Animals; D001483:Base Sequence; D001710:Biotin; D009033:Culicidae / Q000469:parasitology*; D015342:DNA Probes; D016054:DNA, Protozoan / Q000032:analysis*; D003890:Desiccation; D007553:Isotope Labeling; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000302:isolation & purification*; D012091:Repetitive Sequences, Nucleic Acid; D012680:Sensitivity and Specificity
D015342:DNA Probes; D016054:DNA, Protozoan; D001710:Biotin