Day of estrous cycle affects follicular dynamics after induced luteolysis in ewes.
Effects of day of estrous cycle and induced luteal regression on follicular dynamics in ewes were examined. In Exp. 1, ewes received PGF2 alpha (i.m.) on d 5, 8, or 11 after estrus (d 0) or saline on d 14 (n = 27, 27, 31, and 29, respectively). Ewes were laparotomized before treatment and again 48 h after induced estrus to record numbers, sizes, and locations of follicles > or = 2 mm and corpora lutea (CL). The two largest follicles (designated F1 and F2) and any of equal size (F1.5 and F2.5) were marked with India ink in stroma around the follicle. Ewes treated on d 8 ovulated fewer F1 and F1.5 (39%) than ewes treated on d 5 (69%; P < .05); d 11 (51%) and 14 (56%) were intermediate. In Exp. 2, ewes received PGF2 alpha on d 5 or 8 (n = 15/d). Ovaries were examined with transrectal ultrasonography daily from 4 d before PGF2 alpha until ovulation, and on d 5 and 8 after ovulation. Diameters, locations, and ranks (at time of PGF2 alpha) of follicles > or = 2 mm and CL were recorded. Ewes treated on d 8 ovulated fewer F1 and F1.5 (35%) than ewes treated on d 5 (86%; P < .05). Ovulatory follicles at time of PGF2 alpha were larger on d 5 (4.7 +/- .2 mm) than on d 8 (4.0 +/- .2 mm; P < .05).(ABSTRACT TRUNCATED AT 250 WORDS)
Houghton
J A
JA
Division of Animal and Veterinary Sciences, West Virginia University, Morgantown 26506-6108, USA.
Liberati
N
N
Schrick
F N
FN
Townsend
E C
EC
Dailey
R A
RA
Inskeep
E K
EK
D000818:Animals; D003338:Corpus Luteum / Q000187:drug effects* / Q000502:physiology; D015237:Dinoprost / Q000494:pharmacology*; D004971:Estrus / Q000502:physiology*; D005260:Female; D003341:Luteolysis / Q000187:drug effects*; D006080:Ovarian Follicle / Q000502:physiology*; D010053:Ovary / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology; D010060:Ovulation / Q000502:physiology; D011897:Random Allocation; D012756:Sheep / Q000502:physiology*; D014463:Ultrasonography
D015237:Dinoprost