Cloning and sequencing of the cDNA encoding the avian kidney cAMP-dependent protein kinase inhibitor protein.
The endogenous inhibitor of cAMP-dependent protein kinase (PKA) is down-regulated in the kidneys from vitamin-D-replete chicks as compared to vitamin-D-deficient chicks. Screening of a vitamin-D-deficient chick kidney library resulted in the isolation of a 450-bp cDNA clone encoding the 76-amino acid (aa) protein kinase inhibitor (PKI). The deduced aa sequence of avian PKI shares 80 and 41% identity with the mammalian PKI alpha and PKI beta 1 isoforms, respectively. The chick and mammalian PKI contain conserved N-terminal sequences, including the pseudo-substrate site (18GRRNA22), which are required for potent inhibition of the catalytic subunit of PKA. Chick kidney PKI contains ten unique aa in the C-terminal portion of the protein that are not shared with the mammalian PKI alpha or beta isoforms.
Marchetto
G S
GS
Department of Biochemistry, University of California, Riverside 92521, USA.
Henry
H L
HL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors*; D018076:DNA, Complementary / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins; D007668:Kidney; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; C015482:protein kinase modulator; D017868:Cyclic AMP-Dependent Protein Kinases