Cloning and characterization of senC, a gene involved in both aerobic respiration and photosynthesis gene expression in Rhodobacter capsulatus.
The purple nonsulfur photosynthetic eubacterium Rhodobacter capsulatus is a versatile organism that can obtain cellular energy by several means, including the capture of light energy for photosynthesis as well as the use of light-independent respiration, in which molecular oxygen serves as a terminal electron acceptor. In this study, we have identified and characterized a novel gene, senC, mutations in which affect respiration as well as the induction of photosynthesis gene expression. The protein coded by senC exhibits 33% sequence identity to the yeast nucleus-encoded protein SCO1, which is thought to be a mitochondrion-associated cytochrome c oxidase assembly factor. Like yeast SCO1, SenC is required for optimal cytochrome c oxidase activity in aerobically grown R. capsulatus cells. We further show that senC is required for maximal induction from the puf and puh operons, which encode the structural polypeptides of the light-harvesting and reaction center complexes.
Buggy
J
J
Department of Biology, Indiana University, Bloomington 47405, USA.
Bauer
C E
CE
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D017124:Conserved Sequence; D003576:Electron Transport Complex IV / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005809:Genes, Regulator; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D010101:Oxygen Consumption / Q000235:genetics*; D010788:Photosynthesis / Q000235:genetics*; D045322:Photosynthetic Reaction Center Complex Proteins; D016354:Rhodobacter capsulatus / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D045322:Photosynthetic Reaction Center Complex Proteins; C096879:SenC protein, Rhodobacter capsulatus; D003576:Electron Transport Complex IV