Simultaneous visualization of Q-bands and FISH signals using a novel fluorochrome.
We developed a new HNPP-azo dye method for detection of fluorescence in situ hybridization (FISH) signals on Q-banded chromosomes by use of a newly synthesized fluorochrome, HNPP (3-hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester), which reacts enzymatically with alkaline phosphatase and azo dye. The FISH staining method permits simultaneous detection of orange HNPP signals on chromosomal sites labeled by Q-banding, allowing the assignment of small (440-1,200 bp) probes.
Kondoh
Y
Y
Aisin Cosmos R&D Co., Ltd., Kariya, Aichi, Japan.
Ono
T
T
Kagiyama
N
N
Fujita
S
S
Momiyama
M
M
Hori
S H
SH
Yoshida
M C
MC
D000818:Animals; D001391:Azo Compounds; D002478:Cells, Cultured; D002871:Chromosome Banding / Q000379:methods*; D005456:Fluorescent Dyes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D051379:Mice; D009281:Naphthalenes
D001391:Azo Compounds; D005456:Fluorescent Dyes; D009281:Naphthalenes; C094602:3-hydroxy-N-(2'-biphenyl)-2-naphthalenecarboxamide phosphate