title: Distinct roles of the receptor tyrosine kinases Tie-1 and Tie-2 in blood vessel formation.
abstract: Tie-1 and Tie-2 define a new class of receptor tyrosine kinases that are specifically expressed in developing vascular endothelial cells. To study the functions of Tie-1 and Tie-2 during vascular endothelial cell growth and differentiation in vivo, targeted mutations of the genes in mice were introduced by homologous recombination. Embryos deficient in Tie-1 failed to establish structural integrity of vascular endothelial cells, resulting in oedema and subsequently localized haemorrhage. However, analyses of embryos deficient in Tie-2 showed that it is important in angiogenesis, particularly for vascular network formation in endothelial cells. This result contrasts with previous reports on Tie-2 function in vasculogenesis and/or endothelial cell survival. Our in vivo analyses indicate that the structurally related receptor tyrosine kinases Tie-1 and Tie-2 have important but distinct roles in the formation of blood vessels.
authors.affiliation: Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110, USA.
authors.forename: T N
authors.initials: TN
authors.lastname: Sato
authors.forename: Y
authors.initials: Y
authors.lastname: Tozawa
authors.forename: U
authors.initials: U
authors.lastname: Deutsch
authors.forename: K
authors.initials: K
authors.lastname: Wolburg-Buchholz
authors.forename: Y
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: M
authors.initials: M
authors.lastname: Gendron-Maguire
authors.forename: T
authors.initials: T
authors.lastname: Gridley
authors.forename: H
authors.initials: H
authors.lastname: Wolburg
authors.forename: W
authors.initials: W
authors.lastname: Risau
authors.forename: Y
authors.initials: Y
authors.lastname: Qin
mesh_terms: D000818:Animals; D001483:Base Sequence; D001808:Blood Vessels / Q000196:embryology* / Q000254:growth & development; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002460:Cell Line; D017931:DNA Primers; D004487:Edema / Q000196:embryology; D004730:Endothelium, Vascular / Q000166:cytology / Q000196:embryology*; D006470:Hemorrhage / Q000196:embryology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010641:Phenotype; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000502:physiology*; D042810:Receptor, TIE-1; D042787:Receptor, TIE-2; D042764:Receptors, TIE
chemical_list: D017931:DNA Primers; D020794:Receptor Protein-Tyrosine Kinases; D042810:Receptor, TIE-1; D042787:Receptor, TIE-2; D042764:Receptors, TIE