Rare occurrence of p53 and ras gene mutations in preneoplastic and neoplastic mouse endometrial lesions induced by N-methyl-N-nitrosourea and 17 beta-estradiol.
To examine K-, H-, or N-ras and p53 gene mutations in mouse endometrial carcinogenesis induced by N-methyl-N-nitrosourea and 17 beta-estradiol, we performed polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) in 13 adenocarcinomas and 11 other preneoplastic lesions. A significant shifted band in exon 5 of p53 using PCR-SSCP was detected in one of 13 adenocarcinomas. Direct sequencing showed that the mutation was TCA-to-TGA (Ser-to-End) transition. These results suggest that ras gene mutations were not related to carcinogenesis and inactivation of p53 may occur with low frequency during the mouse endometrial carcinogenesis in this model.
Murase
T
T
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
Niwa
K
K
Morishita
S
S
Itoh
N
N
Mori
H
H
Tanaka
T
T
Tamaya
T
T
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D000818:Animals; D001483:Base Sequence; D016889:Endometrial Neoplasms / Q000139:chemically induced* / Q000235:genetics* / Q000473:pathology; D004717:Endometrium / Q000473:pathology; D004958:Estradiol; D005091:Exons; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016158:Genes, p53; D011905:Genes, ras; D006965:Hyperplasia / Q000235:genetics / Q000473:pathology; D008770:Methylnitrosourea; D051379:Mice; D008813:Mice, Inbred ICR; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured
D004958:Estradiol; D008770:Methylnitrosourea