title: Lactate dehydrogenase isoenzymes in mammalian cells exposed to isophenphos.
abstract: The cytotoxicity of isophenphos, an organophosphorus insecticide that has the potential to cause delayed polyneuropathy, was evaluated in GBK and V79 cells. A 72 h time course following isophenphos exposure indicated a dose-dependent growth inhibition as determined by cell counts. The administration of isophenphos (20 g/ml) to GBK cells cultured at high densities indicated a decrease in the activities of LDH isoenzymes. Analysis of V79 cells revealed a decrease of LDH3, the only LDH isoenzyme detected in these cells.
authors.affiliation: Instituto Biologico, São Paulo, Brazil.
authors.forename: M A
authors.initials: MA
authors.lastname: Rodrigues
authors.forename: M J
authors.initials: MJ
authors.lastname: Fernandes
authors.forename: M D
authors.initials: MD
authors.lastname: Angelo
mesh_terms: D000818:Animals; D002417:Cattle; D002452:Cell Count / Q000187:drug effects; D002460:Cell Line; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D007306:Insecticides / Q000633:toxicity*; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000187:drug effects*; D009946:Organothiophosphorus Compounds / Q000633:toxicity*
chemical_list: D007306:Insecticides; D007527:Isoenzymes; D009946:Organothiophosphorus Compounds; C035019:isofenphos; D007770:L-Lactate Dehydrogenase