title: Cloning and sequence analysis of caprine N-acetylglucosamine 6-sulfatase cDNA.
abstract: Mucopolysaccharidosis IIID results from the deficiency of N-acetylglucosamine 6-sulfatase activity. A Nubian goat with this lysosomal storage disease has been identified. As a first step in developing this animal model for testing treatment methods, we cloned and sequenced the caprine N-acetylglucosamine 6-sulfatase cDNA coding region. Overall there is 88% nucleotide homology between the goat and human sequence and 94% homology of the deduced amino acid sequence. The human and two ruminant species differ by the presence of an imperfect trinucleotide (CCG) repeat in the ruminant signal sequence.
authors.affiliation: Department of Pathology, Michigan State University, East Lansing 48824-1314, USA.
authors.forename: K
authors.initials: K
authors.lastname: Friderici
authors.forename: K T
authors.initials: KT
authors.lastname: Cavanagh
authors.forename: J R
authors.initials: JR
authors.lastname: Leipprandt
authors.forename: C E
authors.initials: CE
authors.lastname: Traviss
authors.forename: D S
authors.initials: DS
authors.lastname: Anson
authors.forename: J J
authors.initials: JJ
authors.lastname: Hopwood
authors.forename: M Z
authors.initials: MZ
authors.lastname: Jones
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry*; D006041:Goats / Q000235:genetics*; D008969:Molecular Sequence Data; D009084:Mucopolysaccharidosis III / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013429:Sulfatases / Q000172:deficiency / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D013429:Sulfatases; C018719:N-acetylglucosamine-6-sulfatase