title: [Genetic structure of soil population of the fungus Fusarium oxysporum Schlechtend.: Fr.: molecular reidentification of the species and genetic differentiation of isolates using polymerase chain reaction with universal primers (UP-PCR)].
abstract: The genetic structure of three soil populations of fungus Fusarium oxysporum was analyzed using polymerase chain reaction with universal primers (UP-PCR). Distinct UP-PCR variants revealed by means of cross-dot hybridization of amplified DNA and restriction analysis of nuclear ribosomal DNA represent subspecies or sibling species of F. oxysporum. The remaining isolates of F. oxysporum showed moderate UP-PCR polymorphism characterized by numerous types, whose relatedness was analyzed by computer treatment of the UP-PCR patterns. The genetic distance trees based on the UP-PCR patterns, which were obtained with different universal primers, demonstrated similar topology. This suggests that evolutionarily important genome rearrangements correlatively occur within the entire genome. Isolates representing different UP-PCR polymorphisms were encountered in all populations, being distributed asymmetrically in two of these. In general, soil populations of F. oxysporum were represented by numerous genetically isolated groups with a similar genome structure. The genetic heterogeneity of the isolates within these groups is likely to be caused by the parasexual process. The usefulness of the UP-PCR technique for population studies of F. oxysporum was demonstrated.
authors.forename: S A
authors.initials: SA
authors.lastname: Bulat
authors.forename: N V
authors.initials: NV
authors.lastname: Mironenko
authors.forename: Iu G
authors.initials: IuG
authors.lastname: Zholkevich
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D005670:Fusarium / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012988:Soil Microbiology
chemical_list: D017931:DNA Primers