Naturally occurring motoneuron cell death in rat upper respiratory tract motor nuclei: a histological, fast DiI and immunocytochemical study of the nucleus ambiguus.
The mammalian upper respiratory tract (URT) serves as the common modality for aspects of respiration, deglutition, and vocalization. Although these actions are dependent on coordinated and specific neuromuscular control, little is known about the development of URT control centers. As such, this study investigated the occurrence of naturally occurring motoneuron cell death (MCD) in the nucleus ambiguus (NA) of a developmental series of rats. Standard histological techniques were used to count motoneurons in the ventrolateral brainstem where the mature NA is found. In addition, the neural tracer, fast DiI, was used to determine whether motoneurons were still migrating into the region of the NA during the period that cell counts were first taken. Furthermore, to elucidate the potential effect of inadvertently counting large interneurons on the assessment of motoneuron numbers, an antibody to gamma-aminobutyric acid (GABA) was used. The results of this study have, for the first time, demonstrated that MCD occurs in a URT-related motor nucleus. Approximately a 50% cell death was observed during the prenatal development of NA, with no further loss seen postnatally. The fast DiI studies showed that by embryonic day 17, NA was fully formed, suggesting that motoneuron migration from the basal plate was complete. In addition, use of the GABA antibody showed a lack of inhibitory interneurons within the NA. The finding of MCD in the NA helps define a critical period in the formation of URT neuromuscular control. As the course of MCD is modifiable by epigenetic signals, insult to the organism during this prenatal period may compromise future URT control.
Friedland
D R
DR
Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York, New York 10029, USA.
Eden
A R
AR
Laitman
J T
JT
D000818:Animals; D001933:Brain Stem / Q000196:embryology / Q000254:growth & development / Q000473:pathology*; D002232:Carbocyanines; D016923:Cell Death / Q000502:physiology; D005456:Fluorescent Dyes; D007150:Immunohistochemistry; D007395:Interneurons / Q000502:physiology; D007823:Laryngeal Nerves / Q000196:embryology / Q000254:growth & development / Q000473:pathology*; D009046:Motor Neurons / Q000166:cytology*; D010614:Pharynx / Q000294:innervation*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012137:Respiratory System / Q000166:cytology*; D005680:gamma-Aminobutyric Acid / Q000502:physiology
D002232:Carbocyanines; D005456:Fluorescent Dyes; C024286:3,3'-dioctadecylindocarbocyanine; D005680:gamma-Aminobutyric Acid