title: Direct and competitive kinetic analysis of the interaction between human IgG1 and a one domain analogue of protein A.
abstract: Direct and competitive kinetic analysis of the binding between a one domain analogue of protein A, and mutants thereof, to immobilised hIgG1 was compared using a biosensor system based on surface plasmon resonance detection. Rate constants determined from both assays were almost identical. The experiments demonstrate that competitive kinetic analysis can be used in combination with biosensor technology, and indicate that competitive kinetic analysis may extend the use of the technology to include low molecular weight analytes.
authors.affiliation: Pharmacia Biosensor AB, Uppsala, Sweden.
authors.forename: R
authors.initials: R
authors.lastname: Karlsson
authors.forename: L
authors.initials: L
authors.lastname: Jendeberg
authors.forename: B
authors.initials: B
authors.lastname: Nilsson
authors.forename: J
authors.initials: J
authors.lastname: Nilsson
authors.forename: P A
authors.initials: PA
authors.lastname: Nygren
mesh_terms: D000486:Allergy and Immunology / Q000295:instrumentation; D001667:Binding, Competitive; D015374:Biosensing Techniques; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D013205:Staphylococcal Protein A / Q000737:chemistry* / Q000378:metabolism
chemical_list: D007074:Immunoglobulin G; D013205:Staphylococcal Protein A