Molecular cloning and functional characterization of human lymphotactin.
We describe the isolation of a cDNA that encodes human lymphotactin (Ltn), a new class of lymphocyte-specific chemokine. Human Ltn shows similarity to some members of the C-C chemokine family but has lost the first and third cysteine residues that are characteristic of the C-C and C-X-C chemokines. Ltn is chemotactic for lymphocytes but not for monocytes, a characteristic that makes it unique among chemokines. In addition, calcium flux desensitization studies indicate that Ltn uses a unique receptor. The human Ltn gene maps to a different chromosome than do the C-C and C-X-C chemokine families. Taken together, these characteristics indicate that Ltn is the first example of a new class of human chemokines with preferential effects on lymphocytes.
Kennedy
J
J
Department of Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA.
Kelner
G S
GS
Kleyensteuber
S
S
Schall
T J
TJ
Weiss
M C
MC
Yssel
H
H
Schneider
P V
PV
Cocks
B G
BG
Bacon
K B
KB
Zlotnik
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002118:Calcium / Q000378:metabolism; D019744:Chemokines, C; D002634:Chemotaxis, Leukocyte / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D015723:Gene Library; D006801:Humans; D008222:Lymphokines / Q000032:analysis / Q000235:genetics* / Q000502:physiology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis; D012795:Sialoglycoproteins / Q000032:analysis / Q000235:genetics* / Q000502:physiology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D019744:Chemokines, C; D008222:Lymphokines; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; C092390:XCL1 protein, human; C018997:lymphotactin; D002118:Calcium