title: On the regulation and diversity of restriction in Escherichia coli.
abstract: The effect of UV irradiation on restriction mediated by four endogenous restriction systems of E. coli K-12 was investigated using a uniform testing method. Restriction by all four systems was reduced when treated cells were separately challenged with lambda phage carrying modification patterns that elicit restriction by each system. The response of each system was genetically and physiologically distinct.
authors.affiliation: New England Biolabs, Beverly, MA 01915, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Kelleher
authors.forename: E A
authors.initials: EA
authors.lastname: Raleigh
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism / Q000528:radiation effects; D010582:Bacteriophage lambda / Q000502:physiology; D004262:DNA Restriction Enzymes / Q000378:metabolism* / Q000528:radiation effects; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial / Q000528:radiation effects*; D015971:Gene Expression Regulation, Enzymologic / Q000528:radiation effects*; D010088:Oxidoreductases; D015534:Trans-Activators; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D015534:Trans-Activators; C088146:mcrB protein, Streptomyces lavendulae; D010088:Oxidoreductases; C088145:mcrA protein, Streptomyces lavendulae; D004262:DNA Restriction Enzymes; C070141:Mrr protein, E coli; C089629:endodeoxyribonuclease EcoKI