title: A note on the development of a non-aldehyde fixation technique for examining spores of Bacillus subtilis under the electron microscope.
abstract: Various techniques were studied for fixing spores of Bacillus subtilis prior to examining them by transmission or scanning electron microscopy. A non-aldehyde technique employing carbodiimide in cacodylate buffer produced excellent results and could be of value in studying the cytological changes produced in spores exposed to inimical treatments.
authors.affiliation: Welsh School of Pharmacy, University of Wales College of Cardiff, UK.
authors.forename: A G
authors.initials: AG
authors.lastname: Knott
authors.forename: A C
authors.initials: AC
authors.lastname: Hann
authors.forename: A D
authors.initials: AD
authors.lastname: Russell
mesh_terms: D001412:Bacillus subtilis / Q000648:ultrastructure*; D001431:Bacteriological Techniques; D002234:Carbodiimides; D005404:Fixatives; D008854:Microscopy, Electron / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D013171:Spores, Bacterial / Q000648:ultrastructure
chemical_list: D002234:Carbodiimides; D005404:Fixatives