title: Synthesis and anti-HIV activity of 1,1'-dideoxygossypol and related compounds.
abstract: 1,1'-Dideoxygossypol (DDG), 1,1'-dideoxygossylic acid (DDGA), 8-deoxyhemigossypol (DHG), and 8-deoxyhemigossylic acid (DHGA) were synthesized and tested for their ability to inhibit the replication of HIV in vitro. The EC50 for DDGA was < 1 microM, and its threshold cytotoxicity was approximately 20 microM. DDG was less effective than DDGA against HIV and showed considerable toxicity at 5 microM. DHGA was ineffective against HIV and had very low cytotoxicity. DHG showed some anti-HIV activity, but the threshold cytotoxicity was 5 microM. The dissociation constants for the binding of the four compounds to human serum albumin were determined by fluorescence quenching titrations, and all four were found to have much lower affinities for albumin than the parent compound gossypol.
authors.affiliation: Department of Biochemistry, University of New Mexico School of Medicine, Albuquerque 87131, USA.
authors.forename: R E
authors.initials: RE
authors.lastname: Royer
authors.forename: L M
authors.initials: LM
authors.lastname: Deck
authors.forename: T J
authors.initials: TJ
authors.lastname: Vander Jagt
authors.forename: F J
authors.initials: FJ
authors.lastname: Martinez
authors.forename: R G
authors.initials: RG
authors.lastname: Mills
authors.forename: S A
authors.initials: SA
authors.lastname: Young
authors.forename: D L
authors.initials: DL
authors.lastname: Vander Jagt
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000138:chemical synthesis* / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D006072:Gossypol / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology; D015497:HIV-1 / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
chemical_list: C094630:1,1'-dideoxygossypol; D000998:Antiviral Agents; D012709:Serum Albumin; D006072:Gossypol