Crystallization and preliminary X-ray analysis of a lipase from Staphylococcus hyicus.
Single cyrstals of a lipase from Staphylococcus hyicus have been obtained using a combination of 18 to 24% dimethylsulfoxide and 10% isopropanol as a precipitant. The crystals grow at 4 degrees C in 2-3 months. They belong to the orthorhombic space group P212121 with a = 73.31 A, b = 77.96 A, and c = 169.81 A, with two protein molecules per asymmetrical unit. The crystals diffract to at least 2.8 A resolution and are suitable for an X-ray structure analysis.
Ransac
S
S
Laboratory of Biophysical Chemistry, University of Groningen, The Netherlands.
Blaauw
M
M
Dijkstra
B W
BW
Slotboom
A T
AT
Boots
J W
JW
Verheij
H M
HM
D003460:Crystallization; D018360:Crystallography, X-Ray; D004121:Dimethyl Sulfoxide; D008049:Lipase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D013210:Staphylococcus / Q000201:enzymology*
D008049:Lipase; D004121:Dimethyl Sulfoxide