title: PCR detection of the lactocin S structural gene in bacteriocin-producing lactobacilli from meat.
abstract: The lactocin S structural gene (lasA) in seven bacteriocinogenic lactobacilli isolated from fermented sausages was studied. Two degenerate primers were synthesized to amplify a 75-bp fragment of the gene. Three strains amplified the fragment from their plasmid DNA, and hybridization analysis confirmed these results.
authors.affiliation: Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Rodríguez
authors.forename: L M
authors.initials: LM
authors.lastname: Cintas
authors.forename: P
authors.initials: P
authors.lastname: Casaus
authors.forename: A
authors.initials: A
authors.lastname: Suárez
authors.forename: P E
authors.initials: PE
authors.lastname: Hernández
mesh_terms: D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000235:genetics*; D001483:Base Sequence; D005516:Food Microbiology; D005798:Genes, Bacterial; D007778:Lactobacillus / Q000235:genetics*; D008460:Meat; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
chemical_list: D001430:Bacteriocins