Long-term enhancement of Na,K-ATPase pump during blasttransformation of human lymphocytes is controlled first by translational, then by transcriptional mechanisms.
The transition of phytohemagglutinin-activated human lymphocytes from resting state to proliferation is accompanied by a long-term increase in ouabain-sensitive Rb(K) influx which is closely related to a cyclosporin A-sensitive step of G0/G1/S progression. At least two distinct phases of the up-regulation of cation pump has been revealed: the initial stage (5-20 h) which is cycloheximide-inhibitable and actinomycin D (alpha-amanitin)-unaffected, and the later stage (after 20 h) which is cycloheximide- and actinomycin D (alpha-amanitin)-inhibitable. Thus, the enhanced Na,K-ATPase pump during the cell progression from quiescence to proliferation is controlled both at translational and transcriptional levels.
Marakhova
I I
II
Institute of Cytology, Academy of Sciences, St. Petersburg, Russian Federation.
Vereninov
A A
AA
Toropova
F V
FV
Vinogradova
T A
TA
D002453:Cell Cycle; D002455:Cell Division; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation / Q000502:physiology*; D008214:Lymphocytes / Q000166:cytology* / Q000201:enzymology; D010835:Phytohemagglutinins / Q000494:pharmacology; D014176:Protein Biosynthesis; D000254:Sodium-Potassium-Exchanging ATPase / Q000096:biosynthesis*; D014158:Transcription, Genetic
D010835:Phytohemagglutinins; D003609:Dactinomycin; D016572:Cyclosporine; D000254:Sodium-Potassium-Exchanging ATPase