title: Localization of pro-collagen type II mRNA and collagen type II in the healing tooth socket of the rat.
abstract: Sprague-Dawley rats (50 days old) were anaesthetized and the maxillary right molars extracted. The rats were killed at 2, 3, 6, 8 and 10 days after extraction. The maxillae were dissected and prepared for either routine histology, in situ hybridization for pro-collagen type II mRNA, or immunohistochemical detection of collagen type II. Pro-collagen type II mRNA was expressed maximally in the healing tooth socket at 8 days after the extractions, but the protein was not expressed at any time. This suggests that the translation of pro-collagen type II mRNA does not occur in osteoblasts following tooth extraction. Ossification was present in the socket at 6 days after the extractions, which is consistent with the suggestion that an early feature of osteoblastic differentiation may be the expression of type II pro-collagen mRNA.
authors.affiliation: Department of Restorative Dentistry, University Dental Hospital of Manchester, U.K.
authors.forename: H
authors.initials: H
authors.lastname: Devlin
authors.forename: J
authors.initials: J
authors.lastname: Hoyland
authors.forename: A J
authors.initials: AJ
authors.lastname: Freemont
authors.forename: P
authors.initials: P
authors.lastname: Sloan
mesh_terms: D000539:Alveolar Process / Q000737:chemistry* / Q000502:physiology; D000818:Animals; D003094:Collagen / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D007150:Immunohistochemistry; D010006:Osteoblasts / Q000378:metabolism; D011347:Procollagen / Q000096:biosynthesis*; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014081:Tooth Extraction; D014945:Wound Healing / Q000502:physiology*
chemical_list: D011347:Procollagen; D012333:RNA, Messenger; D003094:Collagen