Functional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius.
Sequence analysis of the Streptomyces peucetius daunorubicin biosynthetic gene cluster revealed a partial (dnrQ) and two complete (dnrD and dnrP) open reading frames flanking dnrK. Bioconversion experiments showed that DnrD converts aklanonic acid methylester to aklaviketone and that DnrC is a methyltransferase that converts aklanonic acid to aklanonic acid methylester. The deduced dnrP gene product, homologous to known esterases, may catalyze the conversion of 10-carbomethoxy-13-deoxycarminomycin to its 10-carboxy derivative. The dnrKPQS genes may be transcribed as a polycistronic mRNA.
Madduri
K
K
School of Pharmacy, University of Wisconsin, Madison 53706, USA.
Hutchinson
C R
CR
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System; D003630:Daunorubicin / Q000096:biosynthesis*; D004950:Esterases / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D007535:Isomerases / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C096749:DnrQ protein, Streptomyces peucetius; D012333:RNA, Messenger; D003577:Cytochrome P-450 Enzyme System; D008780:Methyltransferases; C091660:aklanonic acid methyltransferase; C091662:DauP protein, Streptomyces; D004950:Esterases; D007535:Isomerases; C091661:aklanonic acid methyl ester cyclase; D003630:Daunorubicin