Effect of Picibanil (OK-432) on the scavenging effect of free radicals produced during liver regeneration in the rat.
We administered a biological response modifier Picibanil (OK-432), attenuated Streptococcus pyogenes, via the dorsal vein of the penis after 70% hepatectomy in rats, and clarified the scavenging effect of Picibanil on free radicals generated in the regenerating liver. A group of 5 rats was intravenously administered with 25 KE/kg of OK-432 after hepatectomy, while the control group was given saline after hepatectomy. Serum levels of aspartate aminotransferase and alanine aminotransferase and the value of thiobarbituric acid-reactive substances in serum and hepatic tissue after hepatectomy were serially measured, and these values were significantly lower in Picibanil treated animals than in control animals. Free radical production in the regenerating liver was also measured by electron spin resonance spectrometry, and OK-432 injection significantly reduced free radical production. These results suggested that OK-432 reduced hepatocellular damage in regenerating liver by inhibiting lipid peroxidation.
Okamoto
K
K
First Department of Surgery, Okayama University Medical School, Japan.
Hamazaki
K
K
Iwagaki
H
H
Orita
K
K
Mori
A
A
D000410:Alanine Transaminase / Q000097:blood / Q000187:drug effects; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood / Q000187:drug effects; D016166:Free Radical Scavengers / Q000378:metabolism*; D006498:Hepatectomy; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism; D008115:Liver Regeneration / Q000187:drug effects* / Q000502:physiology; D008297:Male; D010844:Picibanil / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D016166:Free Radical Scavengers; D017392:Thiobarbituric Acid Reactive Substances; D010844:Picibanil; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase