title: Copper(II) complexes encapsulated in human red blood cells.
abstract: Copper(II) complexes were encapsulated in human red blood cells in order to test their possible use as antioxidant drugs by virtue of their labile character. ESR spectroscopy was used to verify whether encapsulation in red blood cells leads to the modification of such complexes. With copper(II) complexes bound to dipeptides or tripeptides, an interaction with hemoglobin was found to be present, the hemoglobin having a strong coordinative site formed by four nitrogen donor atoms. Instead, with copper(II) complexes with TAD or PheANN3, which have the greatest stability. ESR spectra always showed the original species. Only the copper(II) complex with GHL gave rise to a complicated behavior, which contained signals from iron(III) species probably coming from oxidative processes. Encapsulation of all copper(II) complexes in erythrocytes caused a slight oxidative stress, compared to the unloaded and to the native cells. However, no significant differences were observed in the major metabolic properties (GSH, glycolytic rate, hexose monophosphate shunt, Ca(2+)-ATPase) of erythrocytes loaded with different copper(II) complexes, with the exception of methemoglobin levels, which were markedly increased in the case of [Cu(GHL)H-1] compared to [Cu(TAD)]. This latter finding suggests that methemoglobin formation can be affected by the type of complex used for encapsulation, depending on the direct interaction of the copper(II) complex with hemoglobin.
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Catania, Italy.
authors.forename: R P
authors.initials: RP
authors.lastname: Bonomo
authors.forename: A
authors.initials: A
authors.lastname: De Flora
authors.forename: E
authors.initials: E
authors.lastname: Rizzarelli
authors.forename: A M
authors.initials: AM
authors.lastname: Santoro
authors.forename: G
authors.initials: G
authors.lastname: Tabbí
authors.forename: M
authors.initials: M
authors.lastname: Tonetti
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D003300:Copper / Q000494:pharmacology*; D004151:Dipeptides / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D006454:Hemoglobins / Q000737:chemistry; D006571:Heterocyclic Compounds / Q000494:pharmacology; D006801:Humans; D007773:Lactates / Q000378:metabolism; D008706:Methemoglobin / Q000378:metabolism; D015394:Molecular Structure; D009842:Oligopeptides / Q000494:pharmacology; D009942:Organometallic Compounds / Q000494:pharmacology*; D018384:Oxidative Stress; D010427:Pentose Phosphate Pathway / Q000187:drug effects; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000494:pharmacology; D011437:Propylamines / Q000494:pharmacology; D013054:Spectrophotometry, Atomic
chemical_list: D000975:Antioxidants; D004151:Dipeptides; D006454:Hemoglobins; D006571:Heterocyclic Compounds; D007773:Lactates; C096134:N,N'-bis(phenylalanyl)-1,3-propanediamine; D009842:Oligopeptides; D009942:Organometallic Compounds; D011437:Propylamines; C024560:cyclam; D010649:Phenylalanine; D003300:Copper; D008706:Methemoglobin; D000252:Calcium-Transporting ATPases; D005978:Glutathione