Unbudded G2 as well as G1 arrest in the stationary phase of the basidiomycetous yeast Cryptococcus neoformans.
Stationary-phase cells of Cryptococcus neoformans displayed two morphological characteristics: virtually all the cells were unbudded even in the early stationary phase and even when grown in rich media, and average cell size increased from that of exponential-phase cells. DNA contents for small and large stationary-phase cells were determined by quantitative fluorescence microscopy after DNA staining with propidium iodide or DAPI. Small cells contained G1 DNA, whereas large unbudded cells had either a G2 or G1 DNA content, indicating that Cr. neoformans can enter into the stationary phase from either the G1 or G2 period.
Takeo
K
K
Department of Fungal Infections, Chiba University, Japan.
Tanaka
R
R
Miyaji
M
M
Nishimura
K
K
D003455:Cryptococcus neoformans / Q000254:growth & development* / Q000378:metabolism; D004271:DNA, Fungal / Q000032:analysis; D016193:G1 Phase; D016195:G2 Phase
D004271:DNA, Fungal