Use of restriction fragment length polymorphism as a genetic marker for typing Mycobacterium avium strains.
Restriction fragment length polymorphism (RFLP) was used to study 75 clinical isolates identified as Mycobacterium avium. Two repetitive insertion sequences, IS1311 and IS900, were used as DNA probes. Although less than 25% of isolates showed RFLP patterns with IS900, all strains gave banding patterns with IS1311. M. avium strains isolated from patients with AIDS exhibited marked polymorphism with both probes.
Roiz
M P
MP
Departamento de Microbiologia, Hospital Universitario 12 de Octubre, Madrid, Spain.
Palenque
E
E
Guerrero
C
C
Garcia
M J
MJ
D017088:AIDS-Related Opportunistic Infections / Q000150:complications / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D015342:DNA Probes / Q000235:genetics; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D008969:Molecular Sequence Data; D015269:Mycobacterium avium Complex / Q000145:classification* / Q000235:genetics*; D015270:Mycobacterium avium-intracellulare Infection / Q000150:complications / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid
D015342:DNA Probes; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D005819:Genetic Markers