Pathogen antigen- and superantigen-reactive synovial fluid T cells in reactive arthritis.
Analysis of pathogen-reactive T cell clones (CD3+4+8-TCR alpha beta +), isolated from the synovial fluid of 2 HLA-B27-positive patients with Yersinia enterocolitica-triggered reactive arthritis, has provided important information about the cellular immune response to this disease-inciting pathogen. This study demonstrates that the proteins secreted by Y. enterocolitica, including a protein with tyrosine phosphatase activity (YopH), are potent immunogens stimulating CD4+ cells within the inflamed joint. The pathogen-reactive T cell clones preferentially utilized a limited set of T cell receptor variable region gene segments. A purified Yersinia superantigen triggered a proliferative response in most of the antigen-reactive T cell clones tested. These results suggest that the activity of this pathogen's superantigen influences the cellular immune response to its antigens.
Lahesmaa
R
R
Department of Leukocyte Biology, Syntex Research, Palo Alto, California, USA.
Soderberg
C
C
Bliska
J
J
Allsup
A
A
Luukkainen
R
R
Steinman
L
L
Uchiyama
T
T
Peltz
G
G
D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D001170:Arthritis, Infectious / Q000276:immunology*; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002999:Clone Cells; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D014644:Genetic Variation; D006680:HLA Antigens / Q000032:analysis; D006650:Histocompatibility Testing; D006801:Humans; D007111:Immunity, Cellular; D016130:Immunophenotyping; D007249:Inflammation; D008213:Lymphocyte Activation; D008297:Male; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000276:immunology; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017027:Protein Tyrosine Phosphatases / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D018089:Superantigens / Q000276:immunology*; D013582:Synovial Fluid / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014774:Virulence / Q000235:genetics; D015009:Yersinia Infections / Q000276:immunology*; D015008:Yersinia enterocolitica / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
D000942:Antigens, Bacterial; D017931:DNA Primers; D004269:DNA, Bacterial; D006680:HLA Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases