[Monitoring of hepatocyte xenotransplantation. Usefulness of various laboratory parameters].
The authors report a study in which they evaluate the efficacy of some laboratory parameters for monitoring intrasplenic hepatocyte xenotransplantation (mouse to rat) as an alternative to 99Tc-HIDA dynamic scan and histologic exam. Swiss mouse and wistar rat hepatocytes were obtained with collagenase digestion. Wistar male rats were used as recipient and were allocated into three groups: A) omotransplanted rats; B) xenotransplanted rats; C) xenotransplanted and immunosuppressed (Cyclosporin A: 20 mg/kg/daily orally) rats. All rats underwent > 70% hepatectomy. Blood samples were obtained daily from a femoral vein and AST, ALT, ALP, bilirubin, albumin and urea were measured. No statistical differences were observed among groups and the laboratory parameters tested can't be considered a valid technique to xenotransplant rejection monitoring.
Lorenzo
M
M
Istituto di Chirurgia Generale e dei Trapianti d'Organo, Facoltà di Medicina e Chirurgia, Università degli Studi di Napoli Federico II, Napoli.
Angrisani
L
L
Triassi
M
M
Ventriglia
R
R
Savino
B
B
Santoro
A
A
Di Salvo
E
E
D000818:Animals; D017690:Cell Transplantation; D016014:Linear Models; D008099:Liver / Q000166:cytology*; D008297:Male; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D014183:Transplantation, Heterologous