title: CheA, CheW, and CheY are required for chemotaxis to oxygen and sugars of the phosphotransferase system in Escherichia coli.
abstract: We carried out studies with Escherichia coli to determine the site at which the methylation-independent pathways for taxis to oxygen and to sugars of the phosphoenolpyruvate:sugar phosphotransferase transport system converge with the methylation-dependent chemotaxis pathways. Using genetic reconstitution of the pathways in a null strain, we determined that all pathways examined required the products of the genes cheA, cheW, and cheY. Thus, we conclude that both the methylation-independent and methylation-dependent pathways converge at CheA, the histidine kinase product of cheA.
authors.affiliation: Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, California 92350, USA.
authors.forename: E H
authors.initials: EH
authors.lastname: Rowsell
authors.forename: J M
authors.initials: JM
authors.lastname: Smith
authors.forename: A
authors.initials: A
authors.lastname: Wolfe
authors.forename: B L
authors.initials: BL
authors.lastname: Taylor
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D002633:Chemotaxis / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology*; D029968:Escherichia coli Proteins; D005632:Fructose / Q000494:pharmacology; D000071677:Histidine Kinase; D008358:Mannose / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics*; D000072236:Methyl-Accepting Chemotaxis Proteins; D010100:Oxygen / Q000494:pharmacology; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; C489103:CheW protein, E coli; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; C000607606:cheY protein, E coli; C051236:CheW protein, Bacteria; D005632:Fructose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D000071677:Histidine Kinase; C000607608:cheA protein, E coli; D008358:Mannose; D010100:Oxygen