title: The SsoII and NlaX DNA methyltransferases: overproduction and functional analysis.
abstract: Overproduction of the NlaX DNA methyltransferase (M.NlaX) in an Escherichia coli host conferred resistance to SsoII restriction endonuclease (R.SsoII) digestion. This suggested an overlap of sequence specificity between M.NlaX and M.SsoII, the latter of which modifies the internal cytosine of the target sequence 5'-CCNGG-3'. A variant of M.NlaX (M.Sso/Nla), containing an N-terminal extension from M.SsoII, was also enzymatically active. Using deletion analysis, the N-terminal 71 amino-acid residues of M.SsoII were shown to be essential for modification activity.
authors.affiliation: Institute of BioMedical Chemistry, Moscow, Russia.
authors.forename: A S
authors.initials: AS
authors.lastname: Karyagina
authors.forename: V G
authors.initials: VG
authors.lastname: Lunin
authors.lastname: Levtchenko IYa
authors.forename: D
authors.initials: D
authors.lastname: Labbé
authors.forename: R
authors.initials: R
authors.lastname: Brousseau
authors.forename: P C
authors.initials: PC
authors.lastname: Lau
authors.forename: I I
authors.initials: II
authors.lastname: Nikolskaya
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D015257:DNA-Cytosine Methylases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli; D007700:Kinetics; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D009343:Neisseria / Q000201:enzymology* / Q000235:genetics; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D011994:Recombinant Proteins; C063974:DNA modification methylase SsoII; D015257:DNA-Cytosine Methylases