[Hematopoietic and lymphoid cell recovery from radiation damage by cytokines].
Radiation therapy causes various degrees of damage to hematopoietic cells. Cepharanthine (CE), a plant alkaloid, showed the most prominent restorative effect on hematopoiesis among the antileukopenia drugs examined in a previous study. Its mechanism of action was further analyzed in this study, and the following results were obtained: Intravenous administration of CE every other day for 10 days after irradiation increased CFU-S two-fold. CE-conditioned medium from bone marrow stroma cells increased CFU-C. Higher concentrations of CE in the culture non-specifically suppressed the growth of various types of cells, including T cells. However, this suppression disappeared in the presence of peritoneal exudate macrophages (PEC). When Con A blasts were co-cultivated on PEC, concentrations between 10(-6) to 10(-12) M promoted growth of both PEC and T cells. 10 pg/ml and 8 pg/ml of IL-1 were produced from 10(6)/ml of PEC and bone marrow stroma cells, respectively, in 24 hrs with CE. A lesser amount was also produced from thymus stroma cells. A detectable amount of IL-6 was produced from CE-stimulated bone marrow stroma cells. These findings suggested that CE acted as a cytokine-inducer on various epithelial cells in the body and the cytokines thus produced stimulated multipotential hematopoietic progenitors and their progeny as growth and/or differentiation factors.
Makidono
R
R
Department of Immunology, School of Medicine, Kurume University.
Makidono
Y
Y
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D044182:Benzylisoquinolines; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D016207:Cytokines / Q000502:physiology*; D005260:Female; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000528:radiation effects*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D052117:Benzodioxoles
D000470:Alkaloids; D044182:Benzylisoquinolines; D016207:Cytokines; C006947:cepharanthine; D052117:Benzodioxoles