A highly conserved red pigment-concentrating hormone precursor in the blue crab Callinectes sapidus.
A cDNA library was established from the eyestalk ganglia of the blue crab Callinectes sapidus. One clone was isolated (644 bp excluding the poly(A) tail) which encodes the red pigment-concentrating hormone (RPCH)-precursor, consisting of the 25 amino acid residue signal peptide, the RPCH, and a 73 amino acid residue RPCH-precursor related peptide. This clone displays high sequence similarity with a clone isolated from an eyestalk cDNA library of the shore crab Carcinus maenas, in accordance with the close phylogenetic relationship between these species. Northern blot experiments indicated the presence of two different mRNA transcripts which hybridized with a specific RPCH-cDNA probe pointing to the possibility of multiple RPCH isoforms in the blue crab. Although crustacean RPCH and the insect adipokinetic hormones (AKH) are structurally related, their precursors show little similarity.
Klein
J M
JM
Department of Cellular and Molecular Biology, University of West Florida, Pensacola 32514-5751, USA.
Mohrherr
C J
CJ
Sleutels
F
F
Jaenecke
N
N
Riehm
J P
JP
Rao
K R
KR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003386:Brachyura; D017124:Conserved Sequence; D018076:DNA, Complementary; D005123:Eye; D017952:Ganglia, Invertebrate / Q000378:metabolism*; D015723:Gene Library; D007447:Invertebrate Hormones / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D009928:Organ Specificity; D011498:Protein Precursors / Q000096:biosynthesis* / Q000737:chemistry; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives; D015347:RNA Probes; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D007447:Invertebrate Hormones; D009842:Oligopeptides; D011498:Protein Precursors; D015347:RNA Probes; C022059:red pigment-concentrating hormone; D011761:Pyrrolidonecarboxylic Acid