The NACP/synuclein gene: chromosomal assignment and screening for alterations in Alzheimer disease.
The major component of the vascular and plaque amyloid deposits in Alzheimer disease is the amyloid beta peptide (A beta). A second intrinsic component of amyloid, the NAC (non-A beta component of amyloid) peptide, has recently been identified, and its precursor protein was named NACP. A computer homology search allowed us to establish that the human NACP gene was homologous to the rat synuclein gene. We mapped the NACP/synuclein gene to chromosome 4 and cloned three alternatively spliced transcripts in lymphocytes derived from a normal subject. We analyzed by RT-PCR and direct sequencing the entire coding region of the NACP/synuclein gene in a group of patients with familial early onset Alzheimer disease. No mutation was found in 26 unrelated patients. Further studies are required to investigate the implication of the NACP/synuclein gene in Alzheimer disease.
Campion
D
D
CHR, Rouen, France.
Martin
C
C
Heilig
R
R
Charbonnier
F
F
Moreau
V
V
Flaman
J M
JM
Petit
J L
JL
Hannequin
D
D
Brice
A
A
Frebourg
T
T
D017668:Age of Onset; D000544:Alzheimer Disease / Q000453:epidemiology / Q000235:genetics*; D000595:Amino Acid Sequence; D000682:Amyloid / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D002874:Chromosome Mapping; D002894:Chromosomes, Human, Pair 4; D004252:DNA Mutational Analysis; D004198:Disease Susceptibility; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D008214:Lymphocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D051381:Rats / Q000235:genetics; D051843:Synucleins
D000682:Amyloid; D009419:Nerve Tissue Proteins; D011498:Protein Precursors; D051843:Synucleins