Successful expression in pollen of various plant species of in vitro synthesized mRNA introduced by particle bombardment.
Gold particles coated with beta-glucuronidase (GUS) mRNA with a 5' cap structure that had been synthesized in vitro were introduced, by use of a pneumatic particle gun, into pollen grains of lily (Lilium longiflorum), freesia (Freesia refracta) and tulip (Tulipa gesneriana). A fluorometric assay for the GUS activity indicated that in vitro synthesized GUS mRNA introduced into these pollen cells by particle bombardment was successfully expressed. GUS activity in extracts of the bombarded lily pollen became detectable fluorometrically within 30 min after bombardment, peaked at 6 h, then gradually decreased. This activity changed as a function of the developmental stage of the pollen cell of lily.
Tanaka
T
T
Graduate Department of Gene Science, Faculty of Science, Hiroshima University, Japan.
Nishihara
M
M
Seki
M
M
Sakamoto
A
A
Tanaka
K
K
Irifune
K
K
Morikawa
H
H
D015870:Gene Expression; D005966:Glucuronidase / Q000096:biosynthesis* / Q000235:genetics; D008967:Molecular Biology / Q000379:methods*; D011058:Pollen / Q000235:genetics*; D012315:RNA Caps; D012333:RNA, Messenger; D013045:Species Specificity
D012315:RNA Caps; D012333:RNA, Messenger; D005966:Glucuronidase