Induction of differentiation of HL-60 cells by the anti-fungal antibiotic, radicicol.
The anti-fungal antibiotic, radicicol, produced in the culture broth of Neocosmospora tenuicristata, was found to induce differentiation of HL-60 cells into macrophages from the following evidence: (1) it caused morphological changes into macrophage-like cells, (2) induced NBT (Nitrobluetetrazolium) reduction activity, (3) induced phagocytosis, and (4) induced alpha-naphthyl acetate esterase activity. The concentration of radicicol required to differentiate HL-60 cells is 50-100 ng/ml, and the incubation time required for commitment of differentiation is 16 hours. Flow cytometry analysis indicated that radicicol blocks the cell cycle of HL-60 cells at the G1 and G2 sites. In addition, radicicol induced reversal of the transformed phenotype of ras-transformed NIH3T3 cells (DT cells) at 25 ng/ml.
Shimada
Y
Y
Pharmacology and Molecular Biology Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan.
Ogawa
T
T
Sato
A
A
Kaneko
I
I
Tsujita
Y
Y
D000935:Antifungal Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D005434:Flow Cytometry; D018922:HL-60 Cells / Q000187:drug effects*; D006801:Humans; D007783:Lactones / Q000302:isolation & purification / Q000494:pharmacology*; D018942:Macrolides; D009283:Naphthol AS D Esterase / Q000187:drug effects / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects; D014407:Tumor Cells, Cultured
D000935:Antifungal Agents; D007783:Lactones; D018942:Macrolides; D009580:Nitroblue Tetrazolium; D009283:Naphthol AS D Esterase; C035359:monorden