Association of mouse fibrinogen-like protein with murine hepatitis virus-induced prothrombinase activity.
Previously, we demonstrated induction of a unique macrophage prothrombinase during infection of BALB/cJ mice by mouse hepatitis virus strain 3 (MHV-3). By immunologic screening, a clone representing this prothrombinase was isolated from a cDNA library and sequenced. The sequence identified this clone as representing part of a gene, musfiblp, that encodes a fibrinogen-like protein. Six additional clones were isolated, and one clone, p11-3-1, encompassed the entire coding region of musfiblp. Murine macrophages did not constitutively express musfiblp but, when infected with MHV-3, synthesized musfiblp-specific mRNA. musfiblp mRNA induction was earlier and significantly greater in BALB/cJ than A/J macrophages. Prothrombinase activity was demonstrated when musfiblp was expressed from p11-3-1 in RAW 264.7 cells. These data suggest that musfiblp encodes the MHV-induced prothrombinase.
Parr
R L
RL
Department of Pathology, University of Texas Medical School at Houston 77225, USA.
Fung
L
L
Reneker
J
J
Myers-Mason
N
N
Leibowitz
J L
JL
Levy
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D004279:DNA, Viral; D004790:Enzyme Induction; D005340:Fibrinogen; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D006517:Murine hepatitis virus / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013925:Thromboplastin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
D004279:DNA, Viral; C490637:Fgl2 protein, mouse; D012333:RNA, Messenger; D005340:Fibrinogen; D013925:Thromboplastin