Interleukin-3, erythropoietin, and prolactin activate a STAT5-like factor in lymphoid cells.
Interleukin-3 (IL-3)-, erythropoietin (EPO)-, and prolactin (PRL)-induced signal transduction via the JAK/STAT pathway was studied in the IL-3-dependent BAF-3 lymphoid cell line. Transfected cells expressing either the long form of the PRL receptor or the EPO receptor were used. We demonstrated that IL-3, EPO, and PRL activated a transcription factor related to the mammary transcription factor STAT5 but not to STAT1, -2, -3, or -4 as opposed to interferon gamma (IFN gamma) which activated STAT1 in the same cells. Similarly, PRL and EPO activated a STAT5-like factor (STAT5-L) in the rat Nb2 and the human UT7 cells expressing endogenous PRL and EPO receptors, respectively. The hematopoietic STAT5-L activated by IL-3, EPO, or PRL was identified as a 97-kDa tyrosine-phosphorylated protein. These results confer to STAT5 a much broader role than previously suggested.
Pallard
C
C
U363 INSERM, Institut Cochin de Génétique Moléculaire, Hopital Cochin, Paris, France.
Gouilleux
F
F
Charon
M
M
Groner
B
B
Gisselbrecht
S
S
Dusanter-Fourt
I
I
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004921:Erythropoietin / Q000494:pharmacology*; D007377:Interleukin-3 / Q000494:pharmacology*; D008214:Lymphocytes / Q000378:metabolism*; D008894:Milk Proteins; D008969:Molecular Sequence Data; D011388:Prolactin / Q000494:pharmacology*; D011485:Protein Binding; D051381:Rats; D017467:Receptors, Erythropoietin / Q000235:genetics / Q000378:metabolism; D016185:Receptors, Interleukin-3 / Q000378:metabolism; D011981:Receptors, Prolactin / Q000235:genetics / Q000378:metabolism; D050799:STAT5 Transcription Factor; D015398:Signal Transduction; D013347:Subcellular Fractions / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism*; D014162:Transfection
D004268:DNA-Binding Proteins; D007377:Interleukin-3; D008894:Milk Proteins; D017467:Receptors, Erythropoietin; D016185:Receptors, Interleukin-3; D011981:Receptors, Prolactin; D050799:STAT5 Transcription Factor; D015534:Trans-Activators; D004921:Erythropoietin; D011388:Prolactin