Heat shock protein 70 and HLA-DR molecules tissue expression. Prognostic implications in colorectal cancer.
OBJECTIVE
The expression of 70,000-Da heat shock protein (HSP 70) and HLA-DR molecules on cancer cells influences immunologic mechanisms that may be of some prognostic significance. The purpose of this study was to examine the relationship among immunohistochemical HSP 70, HLA-DR expression, and clinicopathologic tumor variables, as well as patient survival in a series of 128 colorectal carcinomas.


METHODS
A three-step immunoperoxidase staining technique was undertaken for detection of both markers.


RESULTS
Of the examined carcinomas 77.3 percent were HSP 70-positive and 74.2 percent were HLA-DR-positive. Increased HSP 70-positive expression correlated significantly with low differentiation (P < 0.05), showed a tendency to characterize advanced stages of disease, and was clearly associated with worse overall survival (P < 0.05). The highest rate of HLA-DR positivity was demonstrated in early stages and was significantly associated with more favorable prognosis (P < 0.001). HSP 70-positive/HLA-DR-negative patients had worse overall survival compared with the rest (P < 0.001).


CONCLUSIONS
The resulting opposite effects on prognosis of examined markers seem to be related to different pathophysiologic functional roles on tumor immunology.
Lazaris
A C
AC
Department of Pathology, Medical School of Athens University, Hippokration General Hospital, Greece.
Theodoropoulos
G E
GE
Davaris
P S
PS
Panoussopoulos
D
D
Nakopoulou
L
L
Kittas
C
C
Golematis
B C
BC
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000032:analysis; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000401:mortality*; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis*; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D015996:Survival Rate
D014408:Biomarkers, Tumor; D006684:HLA-DR Antigens; D018840:HSP70 Heat-Shock Proteins