Cloning of 2.4 kb bovine herpesvirus-1 DNA fragment containing glycoprotein III gene into pUC18 plasmid vector.
A recombinant pBR322 plasmid containing bovine herpesvirus-1 HindIII 'I' fragment was analysed using EcoRI and BamHI restriction endonucleases. This recombinant plasmid was labelled with [alpha 32P]dATP and hybridized with southern blot of HindIII digested BHV-1 DNA fragments. A 2.4 kb double digested EcoRI-BamHI fragment of HindIII 'I' was subcloned into pUC18 plasmid to get complete gIII gene. The recombinant pUC18 plasmid was analysed for 2.4 kb BHV-1 DNA insert by restriction digestion with EcoRI and BamHI. Southern blot of restriction digested plasmid was hybridized with [alpha 32P]dATP labelled BHV-1 DNA probe.
Gupta
P K
PK
Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, India.
Chaudhuri
P
P
Sharma
B
B
Singh
V P
VP
Balain
D S
DS
D000818:Animals; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000302:isolation & purification / Q000378:metabolism*; D015247:Deoxyribonuclease HindIII; D004587:Electrophoresis, Agar Gel; D005822:Genetic Vectors; D007242:Herpesvirus 1, Bovine / Q000235:genetics* / Q000378:metabolism*; D007668:Kidney; D010957:Plasmids; D015183:Restriction Mapping; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*
D004279:DNA, Viral; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins; D015247:Deoxyribonuclease HindIII