Effects of anisodamine against myocardial ischemia-reperfused injuries and antilipid peroxidation.
OBJECTIVE
Anisodamine (Ani), an alkaloid first isolated in China. To study the relationship between the protective effects of Ani on myocardial cells of reperfused injuries and the antilipid peroxidation.


METHODS
Coronary ligation for 15-min followed by 10 min reperfusion was performed in anesthetized rats.


RESULTS
Ani 1, 3, 5 mg.kg-1 i.v. 1 min prior to reperfusion could dose-dependently lower the release of creatine kinase (282 +/- 29, 252 +/- 53, 226 +/- 50), counteract the increase of malondialdehyde content (3.3 +/- 1.3, 3.2 +/- 1.6, 3.1 +/- 1.2) in the reperfused myocardium and preserve the SOD activity (41 +/- 7, 46 +/- 8, 55 +/- 8). Ani completely abolished the drop in the contents of principal unsaturated fatty acids (oleic, linoleic, and arachidonic acids) of the membrane lipids in the reperfused myocardium. SOD 75 U.kg-1 i.v. 1 min prior to reperfusion exerted similar effects like Ani 3 mg.kg-1.


CONCLUSIONS
It is suggested that the antilipid peroxidative effect of Ani may contribute to its protection against reperfusion-induced myocardial injuries.
Yao
X J
XJ
Department of Pharmacology, Fourth Military Medical University, Xi-an, China.
Tan
Y H
YH
Xu
Z C
ZC
Li
X Y
XY
Chen
S Y
SY
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D003402:Creatine Kinase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012991:Solanaceous Alkaloids / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D000889:Anti-Arrhythmia Agents; D012991:Solanaceous Alkaloids; C003922:anisodamine; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D003402:Creatine Kinase