Altered sequence recognition specificity of a C5-DNA methyltransferase carrying a chimeric 'target recognizing domain'.
A MTase with a chimeric TRD with the N-terminal half derived from a TRD recognizing GCNGC, the C-terminal half from one with CCWGG recognition, was constructed. Its target specificity is reported.
Lange
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Max-Planck-Institut für molekulare Genetik, Berlin, Germany.
Wild
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Trautner
T A
TA
D000595:Amino Acid Sequence; D001483:Base Sequence; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D004248:DNA (Cytosine-5-)-Methyltransferases