New restriction endonucleases from thermophilic soil bacteria.
Using the most effective rapid method for the detection of restriction endonucleases (ENases) in microorganisms, 32 thermophilic producers have been isolated. All strains belong to the genus Bacillus. Thermostable isoschizomers of ENases, such as AvaI, BbvI, BbvII, BclI, BsaBI, BsiYI, BsrI, BstEII, BstNI, Cfr10I, ClaI, FspI, HaeIII, HpaII, Ksp632I and SfeI, were isolated.
Repin
V E
VE
State Research Center-ESP Vektor, Koltzovo, Novosibirsk region, Russia.
Lebedev
L R
LR
Puchkova
L
L
Serov
G D
GD
Tereschenko
T
T
Chizikov
V E
VE
Andreeva
I
I
D001407:Bacillus / Q000201:enzymology*; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability; D006358:Hot Temperature; D008969:Molecular Sequence Data; D012988:Soil Microbiology; D013045:Species Specificity
D004262:DNA Restriction Enzymes