Instability of Rickettsia prowazekii RNA polymerase-promoter complexes.
The Rickettsia prowazekii sigma factor was overexpressed, purified, and used to reconstitute RNA polymerase holoenzyme species. R. prowazekii RNA polymerase-promoter complexes were unstable and remained dissociable and heparin sensitive under conditions in which the corresponding Escherichia coli complexes were not. The R. prowazekii core played the major role in determining heparin sensitivity.
Aniskovitch
L P
LP
Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile 36688, USA.
Winkler
H H
HH
D003067:Coenzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology; D009994:Osmolar Concentration; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012283:Rickettsia prowazekii / Q000201:enzymology / Q000235:genetics*; D012808:Sigma Factor / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D003067:Coenzymes; D004269:DNA, Bacterial; D012808:Sigma Factor; D006493:Heparin; D012321:DNA-Directed RNA Polymerases