Inhibitory effect of thyrotropin-releasing hormone on enzyme secretion from isolated rat pancreatic acinar cells.
This study reports a direct effect of TRH on amylase secretion from isolated rat exocrine pancreatic acinar cells. TRH inhibited carbachol (10(-5) M)-stimulated amylase secretion by a maximum of 24% at a concentration of 10(-11) M (p < 0.05), but did not affect basal amylase release in concentrations from 10(-13) M to 10(-8) M. Ceruletide (3 x 10(-10) M)-stimulated amylase secretion was maximally reduced by 23% at a TRH concentration of 10(-10) M (p < 0.05). Direct stimulation of protein kinase C-mediated secretion by the diacylglycerol analogue 1-oleoyl-2-acetyl-sn-glycerol (OAG) was not altered by TRH. The TRH metabolite cyclo (His-Pro) did not influence basal or stimulated pancreatic secretion in vitro. These findings point to a TRH-mediated modulation of exocrine pancreatic secretion at the receptor site.
Kemmer
T P
TP
Rehabilitationsklinik Neresheim, Germany.
Duntas
L
L
Munz
W
W
Malfertheiner
P
P
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002469:Cell Separation; D002108:Ceruletide / Q000494:pharmacology; D004075:Diglycerides / Q000494:pharmacology; D005765:Gastrointestinal Agents / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D010179:Pancreas / Q000166:cytology / Q000201:enzymology*; D010277:Parasympathomimetics / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000378:metabolism / Q000494:pharmacology; D010879:Piperazines / Q000378:metabolism / Q000494:pharmacology; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology*
D004075:Diglycerides; D005765:Gastrointestinal Agents; D010277:Parasympathomimetics; D010456:Peptides, Cyclic; D010879:Piperazines; D013973:Thyrotropin-Releasing Hormone; C038237:1-oleoyl-2-acetylglycerol; D002108:Ceruletide; D002217:Carbachol; D011493:Protein Kinase C; D000681:Amylases; C015924:histidyl-proline diketopiperazine