Genetically determined CD45 variant of value in leucocyte tracing in vivo in the pig.
This paper describes a monoclonal antibody (mAb), anti-pig CD45 (MAC323), that is directed against a polymorphic determinant. A monomorphic anti-pig CD45 mAb (K252.1E4) bound strongly to leucocytes from both MAC323+ and MAC323- pigs, demonstrating the absence of the epitope rather than the CD45 molecule. The MAC323 determinant was present on all leucocytes in positive pigs, exhibiting different expression levels on subsets (monocytes > lymphocytes > polymorphs), but was absent on red blood cells. Pigs lacking this determinant were healthy and grew normally. Careful selection of male and female SLAb/b pigs produced families that were either positive or negative for this epitope. Interbreeding within these families identified the genetic segregation of this variation, which is consistent with the CD45(323) epitope being inherited as a simple dominant autosomal gene. The lack of this determinant in certain lines of inbred pigs has been used to study the homing, lifespan and tissue distribution of donor unlabelled MAC323+ leucocytes and their subsets (using single- and two-colour immunocytological techniques) in MAC323- recipients after either intravenous injection or exchange transfusion. These results, identifying trafficking areas and subsets in constitutive lymphoid and inflammatory tissues, obtained using this genetic marker, extend those obtained previously using fluorescein isothiocyanate (FITC)-labelled donor cells.
Binns
R M
RM
Department of Immunology, Babraham Institute, Cambridge, UK.
Licence
S T
ST
Whyte
A
A
Wilby
M
M
Rothkötter
H J
HJ
Bacon
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology*; D001947:Breeding; D000939:Epitopes / Q000235:genetics / Q000276:immunology*; D005078:Exchange Transfusion, Whole Blood; D005260:Female; D005434:Flow Cytometry; D005799:Genes, Dominant; D005819:Genetic Markers; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000276:immunology*; D007962:Leukocytes / Q000166:cytology / Q000276:immunology*; D016131:Lymphocyte Subsets / Q000502:physiology / Q000637:transplantation; D008297:Male; D051379:Mice; D013552:Swine / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D005819:Genetic Markers; D017493:Leukocyte Common Antigens