title: Comparative study of fibrinogen degradation by four arginine ester hydrolases from the venom of Agkistrodon caliginosus (Kankoku-Mamushi).
abstract: When purified capillary permeability-increasing (CPI)-enzyme-1, CPI-enzyme-2, kininogenase-1 or kininogenase-2 was incubated with human fibrinogen at a ratio of 1:100 by weight, a loss of fibrinogen coagulability was seen at prolonged incubation times. CPI-enzyme-2 and kininogenase-1 caused a rapid loss of coagulability, while CPI-enzyme-1 and kininogenase-2 acted more slowly. When human fibrinogen and each enzyme were incubated at 37 degrees C, CPI-enzyme-2 first cleaved the A alpha-chain then the B beta-chain. The action of CPI-enzyme-1 was similar, but slower. Kininogenase-1 initially caused slow degradation of the B beta-chain than the A alpha-chain, while kininogenase-2 only caused slow cleavage of the A alpha-chain. Although these enzymes did not show thrombin-like activity, CPI-enzyme-2 was able to release fibrinopeptide B faster than fibrinopeptide A, while kininogenase-1 only released fibrinopeptide A. These results indicate that these enzymes differ in their ability to degrade human fibrinogen.
authors.affiliation: Division of Chemistry of Hygiene, Meiji College of Pharmacy, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Shimokawa
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001777:Blood Coagulation; D002265:Carboxylic Ester Hydrolases / Q000494:pharmacology*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003435:Crotalid Venoms / Q000201:enzymology* / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000032:analysis* / Q000378:metabolism*; D005344:Fibrinopeptide A / Q000378:metabolism; D005345:Fibrinopeptide B / Q000378:metabolism; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects
chemical_list: C031442:Agkistrodon venoms; D000596:Amino Acids; D003435:Crotalid Venoms; D005344:Fibrinopeptide A; D005345:Fibrinopeptide B; D005340:Fibrinogen; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases