Secretion of growth hormone (GH)-releasing hormone by GH-producing pituitary adenoma assessed by cell immunoblot analysis.
To detect growth hormone-releasing hormone (GHRH) secreted by monodispersed human GH-producing pituitary adenoma cells, the cell immunoblot assay was performed. GHRH-secreting cells were observed in 3 cases of GH-producing pituitary adenomas. The incidence of GHRH-secreting cells in the total cell populations was 0.24-3.42%. By contrast, no GHRH-secreting cells were observed in 2 cases of clinically nonfunctioning pituitary adenomas. The findings obtained by immunohistochemical studies for adenohypophysial hormones and for nuclear antigens of proliferative activity revealed that the incidence of GHRH-secreting cells is more related to the frequency of GH cells than to the proliferative activity in GH-producing pituitary adenomas.
Kawamoto
K
K
Department of Neurosurgery, Hiroshima University School of Medicine, Japan.
Uozumi
T
T
Arita
K
K
Takechi
A
A
Kawamoto
H
H
D000236:Adenoma / Q000378:metabolism*; D000328:Adult; D005260:Female; D013006:Growth Hormone / Q000378:metabolism*; D013007:Growth Hormone-Releasing Hormone / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D010911:Pituitary Neoplasms / Q000378:metabolism*
D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone