Styrene: from characterisation of DNA adducts to application in styrene-exposed lamination workers.
Styrene oxide, a metabolite of styrene, reacts with many centres in nucleosides but in DNA N-7-, N2- and O6-guanine are the main sites. A 32P-postlabelling method was developed for the detection of O6-styrene oxide DNA adducts from white blood cells. The method involved use of nuclease P1 and magnet transfer. The O6 adducts were detected at a fmol range with about 10% labelling efficiency. In lamination workers the O6 adducts, adjusted for adduct recovery, were detected at a level of 5 adducts/10(8) nucleotides.
Hemminki
K
K
Center for Nutrition and Toxicology, Karolinska Institute, Novum, Huddinge, Sweden.
Vodicka
P
P
D018736:DNA Adducts / Q000378:metabolism*; D004784:Environmental Monitoring / Q000379:methods; D004852:Epoxy Compounds / Q000378:metabolism*; D006801:Humans; D007553:Isotope Labeling; D016273:Occupational Exposure / Q000009:adverse effects*
D018736:DNA Adducts; D004852:Epoxy Compounds; C013690:styrene oxide