Inner ear autoantibodies in patients with sensorineural hearing loss.
In order to identify the inner ear autoantibodies, sera of 195 patients with sensorineural hearing loss and/or vertigo were reached with bovine inner ear antigen by western blotting method. Positive reaction was seen at several molecular weights, in which 33-35 kD, 42 kD and 68 kD were highly reactive. In moderate to severe hearing loss patients, positive reactions with 33-35 kD, 42 kD, 68 kD and others were 25.8%, 26.8%, 26.8% and 71.5% respectively. On the other hand, a positive rate did not clearly correlate with diagnostic entities. Further study to purify inner ear antigen may evaluate inner ear autoimmune disease.
Kosaka
K
K
Department of Otorhinolaryngology, Nippon Medical School, Tokyo, Japan.
Yamanobe
S
S
Tomiyama
S
S
Yagi
T
T
D000818:Animals; D000941:Antigens / Q000737:chemistry / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis*; D002417:Cattle; D007758:Ear, Inner / Q000276:immunology*; D006309:Hearing; D006319:Hearing Loss, Sensorineural / Q000276:immunology* / Q000503:physiopathology; D006801:Humans; D008970:Molecular Weight; D014020:Tissue Extracts / Q000276:immunology; D014717:Vertigo / Q000276:immunology
D000941:Antigens; D001323:Autoantibodies; D014020:Tissue Extracts