title: An efficiently expressed 5.8S rRNA 'tag' for in vivo studies of yeast rRNA biosynthesis and function.
abstract: Inefficient expression or detrimental markers have limited mutational analyses of eukaryotic 5.8S rRNA and the associated rDNA transcribed spacers. We have found a neutral, 4-base insertion mutation that effectively tags the 5.8S rRNA for improved studies of rRNA expression, processing and function. Cells expressing the tagged rDNA plasmid contain 50-60% mutant 5.8S rRNA, but show a normal growth rate and polysomal profile and a constant distribution of tagged 5.8S rRNA. The high level of expression also demonstrates that plasmid-associated rDNA is preferentially transcribed over chromosomal copies.
authors.affiliation: Department of Molecular Biology and Genetics, University of Guelph, Ontario, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Abou Elela
authors.forename: L
authors.initials: L
authors.lastname: Good
authors.forename: R N
authors.initials: RN
authors.lastname: Nazar
mesh_terms: D001483:Base Sequence; D004275:DNA, Ribosomal / Q000032:analysis; D008969:Molecular Sequence Data; D012335:RNA, Ribosomal / Q000032:analysis* / Q000096:biosynthesis / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D016324:Sequence Tagged Sites
chemical_list: D004275:DNA, Ribosomal; D012335:RNA, Ribosomal