Paramyxovirus induced cell fusion. Hemagglutination and hemolysis is enhanced by methylphenidate.
The influence of methylphenidate (MP) upon cell fusion, hemagglutination and hemolysis induced by paramyxovirus was evaluated in vitro. A direct correlation between MP concentrations (range 40 mg/ml to 1.25 mg/ml) and hemagglutination and hemolysis induced by Newcastle disease virus (NDV) was found. Furthermore, MP (500 micrograms/ml) increased formation of the syncytia by LPM paramyxovirus in PK-15 cells. It is fair to speculate that MP leads to activation of the F and/or HN proteins in paramyxovirus by mechanisms other than proteolytic cleavage.
Aglailar Setien
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Unidad de Investigación Médica en Inmunología, Centro Médico Nacional Siglo XXI, México, D.F.
Kretschmer
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Espinosa Larios
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EL
D000818:Animals; D002459:Cell Fusion / Q000187:drug effects*; D002645:Chickens; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D015707:HN Protein / Q000502:physiology; D006387:Hemagglutination, Viral / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D008647:Mesocricetus; D008774:Methylphenidate / Q000494:pharmacology*; D009522:Newcastle disease virus / Q000502:physiology; D018108:Respirovirus / Q000502:physiology*; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000502:physiology
D015707:HN Protein; D014760:Viral Fusion Proteins; D008774:Methylphenidate