Single-cell observation of calcium signals in T cells and antigen-presenting cells during antigen presentation.
Intracellular calcium ion mobilization in T-cell hybridomas and antigen-presenting cells (APC) during the interaction was observed using confocal fluorescence microscopy. No calcium signal was detected in non-activated T-cell hybridomas by antigen presentation. However, in activated T-cell hybridomas, intracellular calcium ion concentration rapidly increased by antigen presentation and thereafter apoptosis was induced. On the contrary, during the interaction with T-cell hybridomas, calcium signal was induced in APCs irrespective of the activation of T-cell hybridomas. Chemical modification of APCs with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, which is known to induce T-cell unresponsiveness during antigen presentation, inhibited cap formation of surface MHC class II molecules and suppressed calcium signals during the interaction with T-cell hybridomas.
Torigoe
C
C
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Tadakuma
T
T
Nakanishi
M
M
D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007424:Intracellular Fluid / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008806:Mice, Inbred AKR; D018613:Microscopy, Confocal; D015398:Signal Transduction / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
D000949:Histocompatibility Antigens Class II; D002118:Calcium