title: A mutation in the pore of the sodium channel alters gating.
abstract: Ion permeation and channel gating are classically considered independent processes, but site-specific mutagenesis studies in K channels suggest that residues in or near the ion-selective pore of the channel can influence activation and inactivation. We describe a mutation in the pore of the skeletal muscle Na channel that alters gating. This mutation, I-W53C (residue 402 in the mu 1 sequence), decreases the sensitivity to block by tetrodotoxin and increases the sensitivity to block by externally applied Cd2+ relative to the wild-type channel, placing this residue within the pore near the external mouth. Based on contemporary models of the structure of the channel, this residue is remote from the regions of the channel known to be involved in gating, yet this mutation abbreviates the time to peak and accelerates the decay of the macroscopic Na current. At the single-channel level we observe a shortening of the latency to first opening and a reduction in the mean open time compared with the wild-type channel. The acceleration of macroscopic current kinetics in the mutant channels can be simulated by changing only the activation and deactivation rate constants while constraining the microscopic inactivation rate constants to the values used to fit the wild-type currents. We conclude that the tryptophan at position 53 in the domain IP-loop may act as a linchpin in the pore that limits the opening transition rate. This effect could reflect an interaction of I-W53 with the activation voltage sensors or a more global gating-induced change in pore structure.
authors.affiliation: Department of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA.
authors.forename: G F
authors.initials: GF
authors.lastname: Tomaselli
authors.forename: N
authors.initials: N
authors.lastname: Chiamvimonvat
authors.forename: H B
authors.initials: HB
authors.lastname: Nuss
authors.forename: J R
authors.initials: JR
authors.lastname: Balser
authors.forename: M T
authors.initials: MT
authors.lastname: Pérez-García
authors.forename: R H
authors.initials: RH
authors.lastname: Xu
authors.forename: D W
authors.initials: DW
authors.lastname: Orias
authors.forename: P H
authors.initials: PH
authors.lastname: Backx
authors.forename: E
authors.initials: E
authors.lastname: Marban
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002104:Cadmium / Q000494:pharmacology; D002462:Cell Membrane / Q000502:physiology / Q000648:ultrastructure; D006063:Chorionic Gonadotropin; D004594:Electrophysiology; D005260:Female; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D007700:Kinetics; D008322:Mammals; D008961:Models, Structural; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism / Q000502:physiology*; D016297:Mutagenesis, Site-Directed; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D010641:Phenotype; D017354:Point Mutation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D015222:Sodium Channels / Q000096:biosynthesis / Q000737:chemistry* / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology; D014981:Xenopus
chemical_list: D006063:Chorionic Gonadotropin; D011994:Recombinant Proteins; D015222:Sodium Channels; D002104:Cadmium; D013779:Tetrodotoxin