A novel rat mesangial matrix protein, MMP-50/100, involved in mesangial glomerulopathies.
BACKGROUND
Within the glomerular extracellular matrix, the glomerular basement membrane and the mesangial matrix have different compositions, presumably related to their different functions. In this study, a novel mesangial matrix protein is recognized by mAb ED5 and KiM4R, which were originally selected for reactivity with follicular dendritic cells of rat lymphoid organs.


METHODS
Distribution of this mesangial matrix protein (MMP-50/100) was studied in normal Wistar rat kidneys by indirect immunofluorescence and immunoelectron microscopy. For partial immunobiochemical characterization, ED5-affinity-purified glomerular matrices were subjected to SDS-PAGE analysis. Expression of MMP-50/100 was additionally studied in kidneys of rats depleted for complement and in kidneys of rats depleted for resident macrophages. Functional significance of MMP-50/100 was studied in kidneys of rats with mesangial glomerulopathies.


RESULTS
Immunoelectron microscopy showed that MMP-50/100 is located in the extracellular matrix of the rat renal mesangium between mesangial cells and the basement membrane and on the mesangial cell membrane. SDS-PAGE analysis of affinity-purified glomerular matrices indicated that MMP-50/100 is a polypeptide glycoprotein with chains of apparent molecular weights of 50 and 100 kDa. Both in vivo and in vitro results indicate that MMP-50/100 does not appear to be a complement factor, or an Fc or complement receptor. In rats partially depleted for resident macrophages, the expression of MMP-50/100 was similar to that in control rats. In rats with BSA-induced chronic serum sickness nephritis, in rats with anti-Thy-1 nephritis, and in rats with uninephrectomy-induced focal glomerular sclerosis, the mesangial expression of MMP-50/100 was significantly increased. In the first model, double-label immunofluorescence demonstrated identical localization of MMP-50/100 with mesangial immune complex deposits.


CONCLUSIONS
We conclude that MMP-50/100 is an intrinsic component of the mesangial matrix, presumably related to the "classic" mesangial cell. Expression of MMP-50/100 is increased in expanded mesangial matrices during development of glomerular disease. Furthermore, MMP-50/100 appears to be involved in the handling of mesangial immune complexes.
Kootstra
C J
CJ
Department of Pathology, University of Leiden, The Netherlands.
Arkema
A
A
Hogendoorn
P C
PC
Daha
M R
MR
Weening
J J
JJ
Parwaresch
M R
MR
Dijkstra
C D
CD
de Heer
E
E
D000818:Animals; D000936:Antigen-Antibody Complex / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins / Q000032:analysis* / Q000502:physiology; D005920:Glomerular Mesangium / Q000737:chemistry* / Q000473:pathology; D005921:Glomerulonephritis / Q000209:etiology*; D051381:Rats; D017208:Rats, Wistar
D000936:Antigen-Antibody Complex; D016326:Extracellular Matrix Proteins