Targeting peptides transiently block a mitochondrial channel.
The effects of synthetic targeting peptides on the activity of the multiple conductance channel (MCC) of mouse and yeast mitochondria were investigated using patch-clamp techniques. Amino-terminal targeting peptides of two inner membrane proteins reversibly decreased the open probability and mean open time of MCC. One of these targeting peptides had no effect on two other voltage-dependent mitochondrial channels. Furthermore, the effects induced by the two targeting peptides on MCC were not elicited by two peptides of an outer membrane protein. The specific interactions of targeting peptides with MCC suggest that this channel may be involved in protein import across the inner mitochondrial membrane.
Lohret
T A
TA
Division of Molecular Medicine, Wadsworth Center, Albany, New York 12201-0509, USA.
Kinnally
K W
KW
D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000187:drug effects / Q000378:metabolism*; D002451:Cell Compartmentation; D004553:Electric Conductivity; D007668:Kidney; D008565:Membrane Proteins / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D021382:Protein Sorting Signals / Q000378:metabolism* / Q000494:pharmacology; D012441:Saccharomyces cerevisiae
D002352:Carrier Proteins; D008565:Membrane Proteins; D021382:Protein Sorting Signals