title: Comparison of hemodialysis versus hemoperfusion in the clearance of high-dose methotrexate in pigs.
abstract: To evaluate the different capabilities of hemo-perfusion (HP) and hemodialysis (HD) treatment in resolving the side effects during high-dose methotrexate (MTX) therapy, an experimental comparative study was done. Under general anesthesia, 12 female Large-White pigs, 35 +/- 5 kg body weight, underwent a bilateral renal vessel ligature to create an acute renal failure and avoid the physiologic elimination of MTX. An MTX i.v. infusion was performed using a dose of 12.5 g/m2 and for a specific time according to its pharmacokinetics. The animals were divided into 2 groups of 6 animals each and connected to the extracorporeal circuit; the first group was submitted to a 1-h HP with an anion exchange resin (Dow 1X-2, Dow Chemical) and the second group to 1-h HD with capillary polysulfone fibers (F6, Fresenius). Blood samples were taken at established intervals for hematological and biochemical evaluations. The results demonstrate a higher capability of HP treatment (P < 0.001) related to HD in MTX removal.
authors.affiliation: Department of Experimental Surgery, Rizzoli Orthopaedic Institute, Bologna, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Giardino
authors.forename: M
authors.initials: M
authors.lastname: Fini
authors.forename: G
authors.initials: G
authors.lastname: Giavaresi
authors.forename: M
authors.initials: M
authors.lastname: Spighi
authors.forename: S
authors.initials: S
authors.lastname: Faenza
authors.forename: M L
authors.initials: ML
authors.lastname: Florio
authors.forename: M
authors.initials: M
authors.lastname: Orlandi
authors.forename: S
authors.initials: S
authors.lastname: Stefoni
mesh_terms: D000818:Animals; D005260:Female; D006464:Hemoperfusion; D008727:Methotrexate / Q000008:administration & dosage* / Q000097:blood / Q000493:pharmacokinetics*; D006435:Renal Dialysis; D013552:Swine
chemical_list: D008727:Methotrexate