Long-term human hematopoiesis in SCID-hu mice bearing transplanted fragments of adult bone and bone marrow cells.
An attempt was made to establish an SCID-hu murine model of long-term human hematopoiesis by coimplantation of a bone fragment and bone marrow (BM) cells from an adult human. The SCID-hu mice were treated with a cytokine mixture (recombinant human stem cell factor, interleukin-3, granulocyte/macrophage colony-stimulating factor, and granulocyte colony-stimulating factor) for 4 months and were then maintained for further 8 months under cytokine-free conditions. In the peripheral blood, spleen, and implanted bone fragments in the SCID-hu mice that had received both a bone fragment and BM cells, human CD59+ cells were detected 1 year after transplantation; however, they were not detectable in SCID-hu mice that had received either a bone fragment or BM cells only. Thus, implantation of both a bone fragment and BM cells appears to provide a model of long-term adult human hematopoiesis in SCID mice.
Heike
Y
Y
Pharmacology Division, National Cancer Center Research Institute, Tokyo, Japan.
Ohira
T
T
Takahashi
M
M
Saijo
N
N
D000368:Aged; D000818:Animals; D001483:Base Sequence; D016026:Bone Marrow Transplantation; D016025:Bone Transplantation; D005260:Female; D005455:Fluorescent Antibody Technique; D006085:Graft Survival; D006410:Hematopoiesis / Q000187:drug effects; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000494:pharmacology; D012272:Ribs / Q000166:cytology / Q000637:transplantation; D014183:Transplantation, Heterologous
D016298:Hematopoietic Cell Growth Factors; D011994:Recombinant Proteins