Confocal microscopy analysis of NPY and TH immunoreactivities in the hypothalamo-hypophysial system of the frog.
Co-expression of tyrosine hydroxylase (TH) and neuropeptide Y (NPY) in local circuits innervating the hypothalamo-pituitary complex of the green frog, Rana ridibunda, was investigated using simultaneous double immunohistochemical technique, aided by dual-channel confocal laser scanning microscopy. NPY and TH immunoreactivities were observed co-occurring within a discrete neuronal population located in the suprachiasmatic region. In other hypothalamic areas, NPY-immunoreactive (IR) perikarya were generally codistributed, but distinct from TH-IR cells. In the adenohypophysial pars intermedia, the overlap between the two markers was partial, demonstrating the existence of multiple neuronal sources for the inputs to the gland.
Battaglia
A A
AA
Department of Animal Biology, University of Torino, Italy.
Feuilloley
M
M
Mulatero
B
B
Beltramo
M
M
Thibault
J
J
Franzoni
M F
MF
Calas
A
A
Vaudry
H
H
Fasolo
A
A
D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D007030:Hypothalamo-Hypophyseal System / Q000737:chemistry* / Q000166:cytology; D008297:Male; D018613:Microscopy, Confocal; D009412:Nerve Fibers / Q000737:chemistry; D009474:Neurons / Q000737:chemistry; D009478:Neuropeptide Y / Q000032:analysis*; D011895:Rana ridibunda; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*
D009478:Neuropeptide Y; D014446:Tyrosine 3-Monooxygenase