A strategy to sequence repetitive DNA based on partial restriction enzyme cleavage.
The strategy to sequence repetitive DNA described in this article is based on partial restriction enzyme cleavage. It is an alternative to using nested deletion with exonuclease III or similar enzymes in which progressively more remote regions of the target DNA are brought into range for sequencing by universal primers.
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Centro de Pesquisas Aggeu Magalhaes-FIOCRUZ, Cidade Universitaria, Recife, Brazil.
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D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004262:DNA Restriction Enzymes; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid
D004247:DNA; D004262:DNA Restriction Enzymes