title: Neutrophil regulation of splanchnic blood flow after hemorrhagic shock.
abstract: OBJECTIVE
  This study examines the hypothesis that neutrophils impair splanchnic blood flow during resuscitation from hemorrhage by inhibiting the release of the compensatory vasodilator PGI2 from the bowel.
  
  
  BACKGROUND
  Resuscitation from hemorrhagic shock is associated with neutrophil infiltration into the intestine, reduced splanchnic perfusion, and reduced release of PGI2 from the intestine.
  
  
  METHODS
  Sprague-Dawley rats received either vinblastine (VIN) to deplete circulating neutrophils or normal saline (NS). These animals then underwent either hemorrhage and resuscitation (SK + R) or sham operation (SHAM). Superior mesenteric artery flow and splanchnic 6-keto PGF1a (metabolite of PGI2) release were measured.
  
  
  RESULTS
  Superior mesenteric artery blood flow was significantly greater in VIN-treated animals sustaining SK + R than in those treated with NS (p < 0.05). Neutrophil depletion preserved 6-keto PGF1a release after SK + R, whereas 6-keto PGF1a release in the NS-treated, SK + R group was significantly reduced (p < 0.05).
  
  
  CONCLUSIONS
  These data are compatible with the hypothesis that neutrophils may influence splanchnic perfusion after SK + R by inhibiting splanchnic PGI2 release.
authors.affiliation: Department of Surgery, University of Texas Southwestern Medical School at Dallas 76235, USA.
authors.forename: R H
authors.initials: RH
authors.lastname: Turnage
authors.forename: K M
authors.initials: KM
authors.lastname: Kadesky
authors.forename: T
authors.initials: T
authors.lastname: Rogers
authors.forename: R
authors.initials: R
authors.lastname: Hernandez
authors.forename: L
authors.initials: L
authors.lastname: Bartula
authors.forename: S I
authors.initials: SI
authors.lastname: Myers
mesh_terms: D000818:Animals; D001011:Aorta; D001794:Blood Pressure / Q000502:physiology; D011464:Epoprostenol / Q000096:biosynthesis; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000473:pathology; D008297:Male; D017538:Mesenteric Artery, Superior; D009504:Neutrophils / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012151:Resuscitation; D012771:Shock, Hemorrhagic / Q000503:physiopathology*; D013152:Splanchnic Circulation / Q000502:physiology*
chemical_list: D011464:Epoprostenol