title: Use of pulsed-field gel electrophoresis for epidemiological study of Escherichia coli O157:H7 during a food-borne outbreak.
abstract: Food and patient isolates from an Escherichia coli O157:H7 outbreak associated with undercooked ground beef were characterized by pulsed-field gel electrophoresis and Shiga-like toxin genotype. Pulsed-field gel electrophoresis confirmed the epidemiologically implicated source of the two-state outbreak and differentiated between outbreak and sporadic strains.
authors.affiliation: Seattle District Laboratory, Food and Drug Administration, Bothell, Washington 98041, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Johnson
authors.forename: S D
authors.initials: SD
authors.lastname: Weagant
authors.forename: K C
authors.initials: KC
authors.lastname: Jinneman
authors.forename: J L
authors.initials: JL
authors.lastname: Bryant
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology*; D005516:Food Microbiology; D006801:Humans; D022641:Shiga Toxin 2
chemical_list: D001427:Bacterial Toxins; D022641:Shiga Toxin 2