Haplotype analysis of intra-specific backcross curly-tail mice confirms the localization of ct to chromosome 4.
We have determined the order of a number of SSR and SSC polymorphic markers that map to distal mouse Chromosome (Chr) 4 and have used analysis of these markers in backcrosses designed to test the localization of the curly-tail (ct) mutation. We have confirmed that ct maps to this region, close to the locus D4Mit69. Our results also support the hypothesis that ct is a semidominant, rather than a recessive, mutation, since we have identified abnormal-tailed mice that are likely to be heterozygous at the ct locus. Finally, we examined Pax7 as a candidate gene for the ct mutation and found no evidence of protein sequence differences in ct compared with wild-type mice.
Beier
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DR
Genetics Division, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
Dushkin
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H
Telle
T
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D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002875:Chromosomes; D003433:Crosses, Genetic; D005260:Female; D008040:Genetic Linkage; D006239:Haplotypes; D018398:Homeodomain Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D008811:Mice, Inbred DBA / Q000235:genetics; D008815:Mice, Inbred Strains / Q000235:genetics; D008817:Mice, Mutant Strains / Q000235:genetics*; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics*; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051763:PAX7 Transcription Factor; D013623:Tail / Q000002:abnormalities*
D018398:Homeodomain Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D051763:PAX7 Transcription Factor; C497322:Pax7 protein, mouse