Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene.
An efficient restriction barrier for methylated DNA in the actinomycete Amycolatopsis methanolica could be avoided by using a nonmethylating Escherichia coli strain for DNA isolations. The A. methanolica prephenate dehydratase gene was cloned from a gene bank in a pMEA300-derived shuttle vector in E. coli and characterized.
Vrijbloed
J W
JW
Department of Microbiology, University of Groningen, Haren, The Netherlands.
van Hylckama Vlieg
J
J
van der Put
N M
NM
Hessels
G I
GI
Dijkhuizen
L
L
D039903:Actinobacteria / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D015698:Genomic Library; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D011302:Prephenate Dehydratase / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014169:Transformation, Bacterial
D011302:Prephenate Dehydratase