title: Prevention of mortality by in vivo depletion of alpha beta T cells in murine lethal listeriosis and involvement of gamma delta T cells in bacterial elimination.
abstract: We previously demonstrated that in murine lethal listeriosis, death is mainly due to massive liver necrosis. In the present study we found that in vivo depletion of alpha beta T cells by administration of anti-TCR beta mAb (H57-597) protected recipient mice from acute mortality and converted lethal listeriosis to sublethal infection. Furthermore, our findings suggested that gamma delta T cells were not involved in the liver necrosis in this condition. After depletion of alpha beta T cells, the number of bacteria decreased gradually to the limit for their detection (10(2) CFU) in 4 wks. Depletion of both alpha beta T cells and gamma delta T cells by administration of anti-TCR beta mAb (H57-597) and anti-TCR delta mAb (3A10) resulted in increased multiplication and persistent presence of bacteria in the liver and spleen. These findings suggest that gamma delta T cells play a significant protective role during infection in mice depleted of alpha beta T cells. In these mice, gamma delta T cells appeared with a peak on day 13 in the liver and on day 20 in the lymph nodes. No increase of gamma delta T cells in the spleen was observed throughout the course of infection.
authors.affiliation: Department of Laboratory Medicine, Nagasaki University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsutake
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: T
authors.initials: T
authors.lastname: Ogino
authors.forename: M
authors.initials: M
authors.lastname: Kaku
authors.forename: E
authors.initials: E
authors.lastname: Nakayama
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D005260:Female; D008089:Listeria monocytogenes / Q000254:growth & development; D008088:Listeriosis / Q000276:immunology / Q000401:mortality* / Q000517:prevention & control*; D008099:Liver / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D018655:Lymphocyte Count; D008212:Lymphocyte Depletion; D051379:Mice; D008809:Mice, Inbred C3H; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta