Identification of enteropathogenic Escherichia coli by PCR-based detection of the bundle-forming pilus gene.
A rapid and simple method of detecting enteropathogenic Escherichia coli (EPEC) was developed. The procedure is based on amplifying by the PCR method a 326-bp region of the bundle-forming pilus gene of EPEC. The oligonucleotide DNA primers used in this procedure did not amplify DNA of any other bacterial enteropathogens tested. The procedure was 100% specific for EPEC strains that exhibit a characteristic pattern of attachment (localized adherence) to HeLa cells.
Gunzburg
S T
ST
Department of Medicine, Cornell University Medical College, New York, New York 10021, USA.
Tornieporth
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NG
Riley
L W
LW
D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology; D005069:Evaluation Studies as Topic; D010861:Fimbriae, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D012703:Serotyping
D017931:DNA Primers; D004269:DNA, Bacterial