Genomic cloning of the human histo-blood group ABO locus.
A P1 phage clone containing the near complete protein coding region of the human histo-blood group ABO gene locus was isolated and characterized. The insert was estimated at approximately 41 kp, and an approximately 20 kbp BamHI fragment contained the near complete coding region as evaluated by Southern blot analysis. Intron/exon boundaries were determined and the coding region was found to span at least seven exons. In situ hybridization to metaphase chromosomes confirmed the predicted localization to 9q34.
Bennett
E P
EP
Faculty of Health Sciences, School of Dentistry, University of Copenhagen, Denmark.
Steffensen
R
R
Clausen
H
H
Weghuis
D O
DO
van Kessel
A G
AG
D000017:ABO Blood-Group System / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D003001:Cloning, Molecular; D017931:DNA Primers; D005091:Exons; D005260:Female; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D013737:Testis / Q000378:metabolism; D014158:Transcription, Genetic
D000017:ABO Blood-Group System; D017931:DNA Primers; D015345:Oligonucleotide Probes