Expression and function of Fc gamma R in mouse mast cells.
Activation of mast cells for the release of secretory granule, membrane lipid, and cytokine mediators via Fc gamma R requires cell surface expression of Fc gamma RIII. A soluble factor(s) from fibroblasts up-regulates the surface expression of Fc gamma RIII in interleukin-3-dependent, bone-marrow-derived mast cells, which otherwise degrade Fc gamma RIII intracellularly. Using a receptor-specific rabbit polyclonal antibody made to a peptide from the cytoplasmic domain of Fc gamma RIII alpha chain, we show that steady-state levels of Fc gamma RIII alpha protein increase in bone-marrow-derived mast cells after coculture with fibroblasts. Thus, the posttranslational stability of this functionally relevant receptor in mast cells is probably regulated by a fibro-blast-derived cytokine(s).
Katz
H R
HR
Department of Medicine, Harvard Medical School, Brigham and Women's Hospital, Boston, MA 02115, USA.
Lobell
R B
RB
D016475:3T3 Cells / Q000378:metabolism; D000818:Animals; D001685:Biological Factors / Q000378:metabolism / Q000494:pharmacology / Q000502:physiology*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D004721:Endoplasmic Reticulum / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology; D007377:Interleukin-3 / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D011499:Protein Processing, Post-Translational; D011940:Receptor Aggregation; D017452:Receptors, IgG / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D015854:Up-Regulation / Q000187:drug effects
D001685:Biological Factors; D007074:Immunoglobulin G; D007377:Interleukin-3; D017452:Receptors, IgG