The isolation and sequence of canine interleukin-2.
A cDNA corresponding to canine IL-2 has been isolated and sequenced. The cDNA was synthesised using RT-PCR, with oligonucleotide primers designed from conserved regions of published IL-2 sequences. The cDNA encodes a predicted full length IL-2 protein of 155 amino-acids. At the nucleic acid level, the canine cDNA shows 92, 88, 88, 82 and 74% homology to published sequences of feline, human, equine, bovine and murine IL-2, respectively. The derived protein shows 90, 86, 85, 76 and 75% similarity to feline, human, equine, murine and bovine IL-2 homologs.
Dunham
S P
SP
Department of Veterinary Pathology, University of Glasgow Veterinary School, UK.
Argyle
D J
DJ
Onions
D E
DE
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004285:Dogs / Q000235:genetics*; D006736:Horses; D006801:Humans; D007376:Interleukin-2 / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
D018076:DNA, Complementary; D007376:Interleukin-2