Escherichia coli adherence to HEp-2 cells with prefixed cells.
We describe a new method which uses cold absolute methanol-prefixed cells for adherence of enteropathogenic Escherichia coli to HEp-2 cells. We found that a method using bacteria grown in Penassay broth to 10(6) to 10(7) CFU/ml and incubated with prefixed cells for 3 h at 37 degrees C, showed 100% sensitivity and specificity against a method using live cells.
Zepeda-Lopez
H M
HM
Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas, Mexico D.F., Mexico.
Gonzalez-Lugo
G M
GM
D001422:Bacterial Adhesion / Q000502:physiology*; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D002460:Cell Line; D003967:Diarrhea / Q000209:etiology; D004926:Escherichia coli / Q000145:classification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000209:etiology; D005069:Evaluation Studies as Topic; D005404:Fixatives; D006801:Humans; D000432:Methanol; D012680:Sensitivity and Specificity; D012703:Serotyping; D014774:Virulence
D005404:Fixatives; D000432:Methanol