title: Mapping of receptor binding sites on IL-1 beta by reconstruction of IL-1ra-like domains.
abstract: Upon structure comparison between IL-1 beta and its antagonist IL-1ra, single or multiple residues along the IL-1 beta sequence were replaced with the corresponding amino acids present in the IL-1ra protein, in the attempt to identify sites important for receptor binding and for biologic activity on the two molecules. Ten of fifteen mutant proteins had activity comparable to that of wild-type IL-1 beta in three different biologic assays and in receptor binding, indicating that the introduced changes did not influence the functional structure of the protein. Conversely, three mutants (SMIL-9: 127/263 R/T-->W/Y; SMIL-10: 125/127/263/265 T/R/T/Q-->R/W/Y/E; SMIL-15:222/227 I/E-->S/S) showed an increased binding capacity for IL-1RI, not paralleled by increased agonist activity, indicating that the introduced IL-1ra residues could be involved in the nonagonist IL-1RI binding site. On the other hand, two mutants showed diminished binding capacity with concomitant decrease in biologic activity. Both mutants (SMIL-1, five substitutions in the loop 202-214; and SMIL-3, total replacement of the loop 164-173 with the IL-1ra stretch 52-55) included substitutions of residues allegedly important for agonist binding to IL-1RI. Mutant SMIL-3 showed the most profound reduction in binding capacity for IL-1RI (CDw121a) and a more than 1,000-fold reduced biologic activity both in vitro and in vivo, but it retained full capacity of binding to IL-1RII (CDw121b) and acted as a selective antagonist of IL-1RII. From these results the following conclusions can be drawn. IL-1 beta binds to IL-1RI and to IL-1RII through different sites, and the loop 164-173 appears as one of the areas involved in the selective interaction with IL-1RI. Agonist (IL-1 beta) and nonagonist (IL-1ra) binding to IL-1RI occur through distinct sites, with loops 164-173 and 202-214 of IL-1 beta identified as two of the sites selectively involved in agonist binding to the activating receptor.
authors.affiliation: Research Center Dompé SpA, L'Aquila, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Boraschi
authors.forename: P
authors.initials: P
authors.lastname: Bossù
authors.forename: P
authors.initials: P
authors.lastname: Ruggiero
authors.forename: A
authors.initials: A
authors.lastname: Tagliabue
authors.forename: R
authors.initials: R
authors.lastname: Bertini
authors.forename: G
authors.initials: G
authors.lastname: Macchia
authors.forename: C
authors.initials: C
authors.lastname: Gasbarro
authors.forename: L
authors.initials: L
authors.lastname: Pellegrini
authors.forename: G
authors.initials: G
authors.lastname: Melillo
authors.forename: E
authors.initials: E
authors.lastname: Ulisse
authors.forename: U
authors.initials: U
authors.lastname: Visconti
authors.forename: C
authors.initials: C
authors.lastname: Bizzarri
authors.forename: E
authors.initials: E
authors.lastname: Del Grosso
authors.forename: A R
authors.initials: AR
authors.lastname: Mackay
authors.forename: G
authors.initials: G
authors.lastname: Frascotti
authors.forename: F
authors.initials: F
authors.lastname: Frigerio
authors.forename: R
authors.initials: R
authors.lastname: Grifantini
authors.forename: G
authors.initials: G
authors.lastname: Grandi
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D005260:Female; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010449:Peptide Mapping; D017472:Receptors, Interleukin-1 / Q000737:chemistry* / Q000378:metabolism; D016415:Sequence Alignment; D017421:Sequence Analysis; D012795:Sialoglycoproteins / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: C507763:IL1RN protein, human; C507764:Il1rn protein, mouse; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D017472:Receptors, Interleukin-1; D012795:Sialoglycoproteins