Studies on the procedure for the construction of cellular protein databases employing micro two-dimensional electrophoresis: an HL-60 protein database.
The procedure to construct a cellular protein database employing a micro two-dimensional electrophoresis (2-DE) technique was studied. Protein information on human cultured leukemic cell HL-60 was collected as an HL-60 protein database, which consists of a 2-DE polypeptide map of 670 recognized spots with spot numbering and information on isoelectric point (pI), molecular mass, and percent integrated density. The procedure employed in this report will be applicable for the comparative studies of cellular proteins and will help to construct comprehensive cellular protein databases.
Manabe
T
T
Department of Material Science, Faculty of Science, Himeji Institute of Technology, Hyogo, Japan.
Yamamoto
H
H
Kawai
M
M
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007526:Isoelectric Point; D015473:Leukemia, Promyelocytic, Acute / Q000378:metabolism*; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis* / Q000737:chemistry; D016622:Silver Staining; D014407:Tumor Cells, Cultured
D009363:Neoplasm Proteins