title: Carcinogen-DNA adducts in human breast tissue.
abstract: Breast cancer is the second leading cause of cancer death among American women. Known risk factors account for only approximately one-third of the 182,000 new cases diagnosed each year in the United States. There is both concern and debate over the contribution of environmental exposures related to lifestyle, occupation, and ambient pollution, particularly in high risk areas such as Long Island, NY and the rest of the northeastern United States. Biomarkers such as carcinogen-DNA adducts can help to explore the role of environmental risk factors for breast cancer by documenting DNA damage from specific carcinogens directly in human tissue. In this pilot study, a total of 31 breast tissue samples were analyzed by the 32P-postlabeling method for carcinogen-DNA adducts characteristic of complex mixtures of aromatic compounds (such as polycyclic aromatic hydrocarbons) and tobacco smoke. The samples included tumor and tumor-adjacent tissues from 15 women with breast cancer and normal tissue samples from 4 women undergoing breast reduction. Among the breast cancer cases, the mean aromatic/hydrophobic-DNA adduct level in all tissues assayed was 5.3 +/- 2.4 (SD) adducts/10(8) nucleotides compared to 2.3 +/- 1.5 among the samples from the noncancer patients. Breast tissue (tumor and/or nontumor) from 30% (5 of 15) of women with breast cancer displayed a pattern of adducts (referred to as a diagonal zone of radioactivity) associated previously, in studies of other tissues, with exposure to tobacco smoke. The 5 positive samples were from current smokers; tissue samples from the 8 nonsmoking cases did not show this characteristic pattern (P < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Environmental Sciences Division, School of Public Health, Columbia University, New York, New York 10032, USA.
authors.forename: F P
authors.initials: FP
authors.lastname: Perera
authors.forename: A
authors.initials: A
authors.lastname: Estabrook
authors.forename: A
authors.initials: A
authors.lastname: Hewer
authors.forename: K
authors.initials: K
authors.lastname: Channing
authors.forename: A
authors.initials: A
authors.lastname: Rundle
authors.forename: L A
authors.initials: LA
authors.lastname: Mooney
authors.forename: R
authors.initials: R
authors.lastname: Whyatt
authors.forename: D H
authors.initials: DH
authors.lastname: Phillips
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000139:chemically induced* / Q000473:pathology; D002274:Carcinogens, Environmental; D002855:Chromatography, Thin Layer; D018736:DNA Adducts / Q000032:analysis*; D004249:DNA Damage; D005260:Female; D006801:Humans; D016462:Mammaplasty; D008875:Middle Aged; D011083:Polycyclic Compounds; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects / Q000473:pathology
chemical_list: D002274:Carcinogens, Environmental; D018736:DNA Adducts; D011083:Polycyclic Compounds