Anti-peptide monoclonal antibodies to the beta-adrenergic receptor: use in purification of beta receptor.
This article describes a new immunopurification procedure based on monoclonal antibodies raised against peptides of the carboxy-terminal region of the turkey beta-adrenergic receptor. This procedure constitutes a significant purification step of recombinant beta-adrenergic receptors expressed in baculovirus-infected Sf9 cells, and allows the recovery of receptors able to activate Gs in phospholipid vesicles. Additionally, this procedure can be combined with affinity chromatography to yield nearly homogeneous receptor.
Luxembourg
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Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, USA.
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D007163:Immunosorbent Techniques; D007202:Indicators and Reagents; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology*; D011943:Receptors, Adrenergic, beta / Q000276:immunology* / Q000302:isolation & purification*; D014422:Turkeys
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; D010446:Peptide Fragments; D011943:Receptors, Adrenergic, beta