Anti-platelet activating factor (PAF) antibody inhibits CFW mouse preimplantation embryo development.
OBJECTIVE
Our purpose was to investigate the effect of anti-PAF antibodies on CFW mouse embryo development in vitro.


METHODS
We studied the in vitro development of CFW mouse one-cell-stage embryos cultured in MEM supplemented with anti-PAF, anti-IgG, or MEM alone to the hatched blastocyst stage.


RESULTS
Mouse embryos cultured with anti-PAF (1:5 dilution; 61%) significantly decreased embryo development compared to controls (MEM alone; 93%), whereas embryos cultured in anti-mouse IgG-supplemented MEM (1:10 dilution; 93%) had no effect.


CONCLUSIONS
The results provide additional evidence that PAF is produced and secreted by cleavage-stage embryos and is required during the preimplantation period.
Roudebush
W E
WE
Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston 29425-2233, USA.
Mathur
S
S
Butler
W J
WJ
D000818:Animals; D000906:Antibodies / Q000276:immunology / Q000494:pharmacology*; D001755:Blastocyst / Q000187:drug effects / Q000276:immunology / Q000502:physiology; D002478:Cells, Cultured; D047108:Embryonic Development / Q000276:immunology*; D005314:Embryonic and Fetal Development / Q000276:immunology*; D005260:Female; D006736:Horses; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010972:Platelet Activating Factor / Q000276:immunology* / Q000378:metabolism / Q000494:pharmacology; D011247:Pregnancy; D012756:Sheep
D000906:Antibodies; D007074:Immunoglobulin G; D010972:Platelet Activating Factor