A novel apparatus that permits multiple routes for infusions and body-fluid collections in a freely-moving animal.
A novel apparatus is described for simultaneous performance of multi-channel infusions/body-fluid collections and multichannel electrical recordings/stimulations in a freely behaving animals. This apparatus consists of a cylindrical cage and other devices described below. Electrical contacts are achieved via a slip-ring commutator. A rotation detector detects the turning of the animal in the cage, and a controller rotates the floor under the animal in the opposite direction by means of a stepping motor. Thus, excessive twisting of the fluid tubing between the animal and experimental equipment is released by rotation of the floor. Floor rotation starts when the animal turns in either direction and exceeds a present number of rotations. When the turning exceeds certain rates, the floor is rotated with higher angular velocities. This floor rotation little affected sleep-wake activities, brain temperature, food and water intake, and general behavior of the rat. Thus, multi-channel fluid routes have been realized; and by excluding the swivel apparatus from the fluid lines, advantages such as no fluid leakage at the swivel, no extra dead volume, and low torque to rotate the device have been gained.
Matsumura
H
H
Osaka Bioscience Institute, Suita, Japan.
Kinoshita
G
G
Satoh
S
S
Osaka
T
T
Hayaishi
O
O
D000818:Animals; D001826:Body Fluids; D001921:Brain / Q000502:physiology; D004326:Drinking; D004435:Eating; D004558:Electric Stimulation; D004576:Electromyography; D006799:Housing, Animal; D017551:Microdialysis; D008991:Monitoring, Physiologic / Q000295:instrumentation; D009043:Motor Activity / Q000502:physiology*; D010477:Perfusion / Q000295:instrumentation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012890:Sleep; D013048:Specimen Handling / Q000295:instrumentation