Lysine oxidation by growing pigs receiving diets containing free and protein-bound lysine.
Knowledge of amino acid availability in feedstuffs is central to accurate diet formulation. Dietary lysine oxidation was evaluated as a means of predicting dietary lysine availability. Growing pigs (30 kg) were offered control (1.43% lysine), free lysine-supplemented soybean meal-, or cottonseed meal-supplemented diets. Supplemented diets provided equivalent total lysine (approximately 24.2 g/d at 30 kg BW) but availabilities of lysine, determined by slope-ratio assay, in the free lysine, soybean meal, and cottonseed meal were 100, 90, and 30%, respectively. Feed was offered in eight equally spaced meals per day to achieve three times maintenance energy intake. Following a meal containing L-[U-14C]lysine (1 mu Ci/kg BW), lysine oxidation, as 14CO2 expired, was lower (P < .05) for the control diet but not different between the other three diets, contrary to the hypothesis. Lysine oxidation following an intravenous bolus dose was lowest (P < .05) for pigs fed the control, highest (P < .05) for pigs fed the cottonseed diet, and intermediate (P < .05) for pigs fed the free lysine-supplemented diet (27.1, 80.2, and 47.5, dpm/kg x 10(-2), respectively). Plasma lysine concentration was lower (P < .05) and lysine specific radioactivity tended to be higher (P < .10) following a meal containing cottonseed than following a meal containing free lysine, indicating that the lysine pool was smaller in pigs receiving the cottonseed-meal diet.(ABSTRACT TRUNCATED AT 250 WORDS)
Ball
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RO
Department of Animal and Poultry Science, University of Guelph, Ontario, Canada.
Batterham
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ES
van Barneveld
R J
RJ
D000821:Animal Feed / Q000032:analysis* / Q000592:standards; D000818:Animals; D001682:Biological Availability; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D003369:Cottonseed Oil / Q000737:chemistry; D004032:Diet / Q000592:standards*; D005260:Female; D008239:Lysine / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics; D010084:Oxidation-Reduction; D013025:Glycine max / Q000737:chemistry; D013552:Swine / Q000254:growth & development* / Q000378:metabolism; D013997:Time Factors
D002250:Carbon Radioisotopes; D003369:Cottonseed Oil; D002245:Carbon Dioxide; D008239:Lysine