title: Chromosome polymorphisms close to the cm-ADE1 locus of candida maltosa.
abstract: The imperfect yeast Candida maltosa has an ill-defined genetic constitution; it is nominally diploid, but probably highly aneuploid, in nature. We report on polymorphisms specifically affecting those chromosomes which bear the cm-ADE1 gene. This gene encodes phosphoribosylaminoimidazole-succino-carboxamide synthetase, an enzyme in the adenine biosynthetic pathway. By electrophoretic karyotype analysis, three differently sized chromosomes were demonstrated to carry cm-ADE1; the size (but not the number) of these chromosomes was also found to vary, both between strains and during the mitotic growth of a single strain. Four different alleles of cm-ADE1 have been cloned and sequenced from one prototrophic strain. DNA sequence divergence between these different alleles is as high as 8%, with the greatest divergence being found in the upstream region. Mitotic recombination events that led to changes in the karyotype were followed by using cm-ADE1 DNA as an hybridization probe. A recombination hot-spot in the neighbourhood of the gene appears to be responsible for the instability of the chromosomes on which it residues.
authors.affiliation: Fachrichtung Biologie, Ernst-Mortiz-Arndt-Universität Greifswald, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Becher
authors.forename: S
authors.initials: S
authors.lastname: Schulze
authors.forename: A
authors.initials: A
authors.lastname: Kasüske
authors.forename: R
authors.initials: R
authors.lastname: Stoll
authors.forename: H
authors.initials: H
authors.lastname: Wedler
authors.forename: S G
authors.initials: SG
authors.lastname: Oliver
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D010453:Peptide Synthases / Q000235:genetics*; D011110:Polymorphism, Genetic; D016415:Sequence Alignment
chemical_list: D010453:Peptide Synthases; C022379:phosphoribosylaminoimidazole-succinocarboxamide synthetase