A biochemical evaluation of oral squamous cell carcinoma growth by measurement of specific activity of succinate dehydrogenase in the subrenal capsule assay.
An auxiliary method for determination of chemosensitivity with the subrenal capsule assay (SRCA) was developed in which the specific activity of succinate dehydrogenase (SD) of tumor implanted beneath the renal capsule is measured. The appropriate conditions for measuring the specific activity of SD were determined. The chemosensitivity of tumors, derived from six xenograft lines originating from oral squamous cell carcinomas, to peplomycin (PEP), cisplatin (CDDP), and 5-fluorouracil (5-FU) were evaluated by the SRCA and the nude mouse assay (NMA). The chemosensitivity evaluated by NMA displayed a higher degree of correlation with that determined by the improved SRCA than with that determined by the conventional SRCA. The correlations between overall accuracy of prediction with the NMA and those with the conventional SRCA and the improved SRCA were 72.2% and 88.9%, respectively. These findings suggest that our new assay may be useful for evaluation of chemosensitivity in the SRCA.
Munakata
H
H
Department of Oral and Maxillofacial Surgery I, Hiroshima University School of Dentistry, Japan.
Kayada
Y
Y
Kawahara
M
M
Sakamoto
T
T
Yoshiga
K
K
Takada
K
K
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy* / Q000201:enzymology; D002945:Cisplatin / Q000627:therapeutic use; D003124:Colorimetry; D004396:Coloring Agents; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D005472:Fluorouracil / Q000627:therapeutic use; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000201:enzymology; D009368:Neoplasm Transplantation; D017663:Peplomycin / Q000627:therapeutic use; D012044:Regression Analysis; D013370:Subrenal Capsule Assay; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles
D000970:Antineoplastic Agents; D004396:Coloring Agents; D013778:Tetrazolium Salts; D013844:Thiazoles; D017663:Peplomycin; D013385:Succinate Dehydrogenase; C022616:thiazolyl blue; D002945:Cisplatin; D005472:Fluorouracil