title: Immunohistochemical localisation of steroidogenic enzymes and phenylethanolamine-N-methyl-transferase (PNMT) in the adrenal gland of the fetal and newborn foal.
abstract: An increase in fetal adrenal cortisol output signals the onset of parturition in many animal species but, in the fetal horse, plasma concentrations of cortisol remain low for much of late pregnancy, with a rise occurring only very close to the time of birth (term 320-360 days). Immunohistochemistry was used to determine the localisation and changes in distribution of key steroidogenic enzymes for cortisol production; P450scc, P450C17 and 3 beta-hydroxysteroid dehydrogenase (3 beta HSD) in adrenal tissue from fetal and newborn horses and these findings were correlated with the appearance of immunoreactive (IR)-phenylethanolamine-N-methyl-transferase (PNMT), a cortisol-dependent enzyme. Five micron sections of adrenal tissue from fetuses at Day 100-156 (n = 5), Day 244-295 (n = 8), greater than Day 300 (n = 4) and from newborn foals (n = 6), were stained using specific antibodies and the avidin-biotin-peroxidase technique. All 3 steroidogenic enzymes were present by Day 150, but in less than 20% of the cortical cells. By late gestation the steroidogenic enzymes were present in approximately 30% of the cells, but the distribution varied. P450SCC and P450C17 predominated in cortical cells proximal to the medulla; 3 beta HSD was present throughout the cortex, but more in the zona fasciculata. In foals after birth, IR-3 beta HSD and IR-P450SCC had increased substantially throughout the adrenal cortex, and IR-P450C17 was present in most cells of the presumptive zonae fasciculata and reticularis. IR-PMNT was localised to nuclei of scattered medullary cells at the medullary-cortical interface by Day 150.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Lawson Research Institute, University of Western Ontario, Department Obstetrics and Gynaecology, London, Canada.
authors.forename: X
authors.initials: X
authors.lastname: Han
authors.forename: A L
authors.initials: AL
authors.lastname: Fowden
authors.forename: M
authors.initials: M
authors.lastname: Silver
authors.forename: N
authors.initials: N
authors.lastname: Holdstock
authors.forename: A J
authors.initials: AJ
authors.lastname: McGladdery
authors.forename: J C
authors.initials: JC
authors.lastname: Ousey
authors.forename: W R
authors.initials: WR
authors.lastname: Allen
authors.forename: P D
authors.initials: PD
authors.lastname: Rossdale
authors.forename: J R
authors.initials: JR
authors.lastname: Challis
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis* / Q000276:immunology; D000311:Adrenal Glands / Q000196:embryology / Q000201:enzymology* / Q000276:immunology; D000446:Aldehyde-Lyases / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000831:Animals, Newborn; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000032:analysis / Q000276:immunology; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000276:immunology; D005333:Fetus / Q000201:enzymology; D006736:Horses / Q000196:embryology / Q000378:metabolism*; D006854:Hydrocortisone / Q000096:biosynthesis*; D007124:Immunoenzyme Techniques; D010625:Phenylethanolamine N-Methyltransferase / Q000032:analysis* / Q000276:immunology; D013254:Steroid 17-alpha-Hydroxylase
chemical_list: D003577:Cytochrome P-450 Enzyme System; D015096:3-Hydroxysteroid Dehydrogenases; D013254:Steroid 17-alpha-Hydroxylase; D002786:Cholesterol Side-Chain Cleavage Enzyme; D010625:Phenylethanolamine N-Methyltransferase; D000446:Aldehyde-Lyases; D006854:Hydrocortisone