Protection by almagate of ethanol-induced gastric mucosal damage in rats.
The study was designed to analyse the protective effects of almagate on a model of gastric injury, ethanol-induced mucosal damage, in which acid plays little, if any, role. Pretreatment with almagate dose-dependently reduced the level of gastric damage induced by oral administration of 1 mL 100% ethanol. Administration of 12 mumol kg-1 alamagate 30 min before ethanol significantly reduced the area of mucosal damage by 65 +/- 10%, and the maximum level of inhibition (74 +/- 11%) was obtained with 150 mumol kg-1 almagate. Administration of higher doses of almagate (200-250 mumol kg-1) did not result in any further increase in the level of protection against ethanol-induced gastric damage. Administration of 1 mL 100% ethanol induces substantial damage to the gastric mucosa, with nearly 40% of the length of the section evaluated exhibiting deep necrotic and haemorrhagic damage. Pretreatment with almagate caused a significant diminution in all parameters of histological damage, whereas damage to the epithelial cell layer was only significantly reduced by pretreatment with the highest doses evaluated (25, 50 and 150 mumol kg-1). Administration of aluminium hydroxide did not modify ethanol-induced mucosal damage, even at doses containing concentrations of aluminium higher than those present in gastroprotective doses of almagate. Pretreatment with sucralfate, another aluminium containing compound, at doses of 250 mumol kg-1 protected the mucosa, although lower doses did not. The present study has shown that almagate prevents ethanol-induced gastric mucosal damage.(ABSTRACT TRUNCATED AT 250 WORDS)
Esplugues
J V
JV
Department of Pharmacology, Faculty of Medicine, University of Valencia, Spain.
Barrachina
M D
MD
Martínez-Cuesta
M A
MA
Calatayud
S
S
Moreno
L
L
Fernandez
A
A
Puig
J
J
Esplugues
J
J
D000284:Administration, Oral; D000536:Aluminum Hydroxide / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000863:Antacids / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002254:Carbonates / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects* / Q000293:injuries; D007213:Indomethacin / Q000008:administration & dosage / Q000494:pharmacology; D008276:Magnesium Hydroxide / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000517:prevention & control*; D013392:Sucralfate / Q000008:administration & dosage / Q000494:pharmacology
D000863:Antacids; D002254:Carbonates; D000431:Ethanol; D013392:Sucralfate; C043698:almagate; D000536:Aluminum Hydroxide; D008276:Magnesium Hydroxide; D007213:Indomethacin