Purification and characterization of two restriction endonucleases isolated from Phormidium inundatum.
We have isolated two restriction endonucleases, PinBI and PinBII, from the cyanobacterial strain Phormidium inundatum, and identified them as isoschizomers of AvaIII and BspMII, respectively.
Piechula
S
S
Department of Microbiology, University of Gdańsk, Poland.
Kur
J
J
Woszczyk
J
J
Podhajska
A J
AJ
D010582:Bacteriophage lambda / Q000378:metabolism; D001483:Base Sequence; D000458:Cyanobacteria / Q000201:enzymology*; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism*; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000302:isolation & purification; D013379:Substrate Specificity
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes; C048503:ATGCAT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C053919:TCCGGA-specific type II deoxyribonucleases