Separation and purification of peptides by high-speed counter-current chromatography.
Preparative separations of peptides have been accomplished using high-speed counter-current chromatography. This has been made possible by the use of a particular solvent system that does not exhibit solvent carryover at high speed flow and centrifugation conditions. The solvent system composed of tert.-butyl methyl ether, n-butanol, acetonitrile and aqueous trifluoroacetic acid which can be adjusted in volume ratios and percent acid is employed in two instruments for counter-current chromatography. The cross-axis coil planet centrifuge was used for separation of various dipeptides. Superior resolution was obtained with the multi-layer coil planet centrifuge in the separation of six dipeptides and in the preparative purification of two synthetic peptides.
Knight
M
M
Peptide Technologies Corporation, Gaithersburg, MD 20877, USA.
Fagarasan
M O
MO
Takahashi
K
K
Geblaoui
A Z
AZ
Ma
Y
Y
Ito
Y
Y
D000595:Amino Acid Sequence; D002498:Centrifugation; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*
D009842:Oligopeptides