[A study on preparation and characteristics of monoclonal antibody against laryngeal carcinoma].
The monoclonal antibody (McAb) to laryngeal squamous carcinoma was prepared by using the technique of B lymph cell hybridomas. After screening, 4 strains of hybridomas which can secrete McAb were obtained and the McAbs in ascites were all collected. Its specificity and biological characteristics were also studied. The results showed that the McAbs can combine with the antigens of standard laryngeal cancer cell strain; the antibody titre were higher than 1: 1280; the McAbs can specifically detect the laryngeal cancer antigen in carcinoma tissues; the McAbs were of high purity and the molecular weight was 100-20kd; the cellular chromosomes of the monoclonal hybridomas were all polyploid.
Guan
G
G
Second Affiliated Hospital, Bethune University of Medical Sciences, Chanchun.
Xu
Y
Y
Sun
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000912:Antibodies, Neoplasm / Q000096:biosynthesis*; D000951:Antigens, Neoplasm / Q000032:analysis; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D005260:Female; D006825:Hybridomas / Q000378:metabolism; D007822:Laryngeal Neoplasms / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm