[The detection of Toxoplasma gondii in abortion tissues of sheep using the polymerase chain reaction].
A polymerase chain reaction was applied to detect Toxoplasma gondii DNA in placental and fetal tissue samples of 47 unselected ovine abortions of the lambing season 1990/91 (Baden-Württemberg, Rhineland, Hesse). For the amplification a 190 bp or 223 bp sequence of the B1-gene of T. gondii was selected as the target sequence. Both sequences were detected in five abortions. All positive results were immunohistochemically confirmed using the peroxidase antiperoxidase technique (PAP-staining). Thus, in Germany, too, T. gondii infection in sheep during pregnancy should be considered as a possible cause of abortions, particularly in case of abortions of unknown genesis.
Steuber
S
S
Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin Berlin.
Niu
A
A
Bauer
C
C
Reetz
J
J
Roth
A
A
Janitschke
K
K
D000034:Abortion, Veterinary / Q000469:parasitology*; D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis*; D005260:Female; D005333:Fetus / Q000469:parasitology; D010920:Placenta / Q000469:parasitology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology*; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000469:parasitology*
D016054:DNA, Protozoan