title: Alternative RNA of epsilon transcripts produces mRNAs encoding two membrane and four secreted IgE isoforms.
abstract: We elucidated a series of alternatively spliced human epsilon mRNAs by employing a human epsilon-specific RT-PCR strategy. Two mRNA isoforms provide for the expression of distinct membrane IgE proteins, the common form of which contains a novel 56-amino-acid sequence after the last epsilon constant-region domain. Other mRNAs encode, in addition to the classic secreted epsilon chain, at least three novel epsilon secreted proteins that lack a transmembrane domain and possess distinctive C-terminal features. Furthermore, the expression of these epsilon mRNA isoforms is differentially regulated by stimuli such as interleukin-10, Fc epsilon RII cross-linking and aromatic hydrocarbons found in diesel exhaust.
authors.affiliation: Hart and Louise Lyon Laboratory, Department of Medicine, UCLA School of Medicine 90024-1680, USA.
authors.forename: A
authors.initials: A
authors.lastname: Saxon
authors.forename: E E
authors.initials: EE
authors.lastname: Max
authors.forename: D
authors.initials: D
authors.lastname: Diaz-Sanchez
authors.forename: K
authors.initials: K
authors.lastname: Zhang
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D006801:Humans; D007073:Immunoglobulin E / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D007073:Immunoglobulin E