Simple interface for microbore LC and electrospray ionization mass spectrometry and analysis of melphalan-alkylation sites in metallothionein.
A microbore high pressure liquid chromatograph has been interfaced to a Vestec electrospray ionization source retrofitted to a Hewlett-Packard quadrupole mass spectrometer. The chief features include the absence of a splitter, the use of a second, ballast column to provide a stable flow rate across the gradient, and an in-line UV detector. The system was evaluated for analysis of peptide mixtures and applied to identification of drug-modified peptides released by tryptic digestion of drug-alkylated metallothionein.
Kaltashov
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IA
University of Maryland Baltimore County, Department of Chemistry and Biochemistry 21228, USA.
Yu
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X
Fenselau
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C
D000477:Alkylating Agents; D000478:Alkylation; D000818:Animals; D002853:Chromatography, Liquid; D006868:Hydrolysis; D013058:Mass Spectrometry; D008558:Melphalan; D008668:Metallothionein / Q000032:analysis*; D008970:Molecular Weight; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D014357:Trypsin
D000477:Alkylating Agents; D010455:Peptides; D008668:Metallothionein; D014357:Trypsin; D008558:Melphalan