title: Brain FOS-like expression in developing and adult honeybees.
abstract: This study was undertaken to examine the expression of the protooncogene c-fos in an invertebrate, the honeybee. We investigated first the expression of Fos-like proteins, testing different methodologies (immunohistochemistry, immunocytochemistry, immunoblotting). These studies were carried out at the embryonic, nymphal and adult stages. We found immunoreactive bands at approximately 30 kD and 57 kD. The first ones are revealed at the embryonic, nymphal and adult stages. The approximately 57 kD proteins are present at the adult stage. Beside this qualitative variation, intensive modification occurs with a burst of protein expression a few days after the insect emergence. The results suggest that Fos-like proteins are found in the developing and maturing nervous system. Basal level of expression is found in the olfactory pathway, convincing to explore the protooncogene c-fos expression in the cellular and molecular analyses of olfactory plasticity.
authors.affiliation: Laboratoire de Neurobiologie Comparée des Invertébrés, INRA-CNRS (URA 1190), Bures-sur-Yvette, France.
authors.forename: C
authors.initials: C
authors.lastname: Fonta
authors.forename: J
authors.initials: J
authors.lastname: Gascuel
authors.forename: C
authors.initials: C
authors.lastname: Masson
mesh_terms: D000818:Animals; D001516:Bees / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D016762:Genes, fos; D015151:Immunoblotting; D007150:Immunohistochemistry; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis*
chemical_list: D016760:Proto-Oncogene Proteins c-fos