Vascular and tubular actions of diazepam in isolated and perfused rat kidney.
The effects of diazepam were studied in the isolated rat kidney under conditions of constant flow. Kidneys were perfused with modified Ringer-Krebs solution. Diazepam produced a raised fractional excretion of water and sodium without hemodynamic changes, suggesting a direct effect on tubular transport mechanisms. Diazepam decreased renal perfusion pressure in a concentration-dependent fashion when kidneys were pretreated with either noradrenaline or potassium chloride. Similar responses were observed when 7-chloro-5-[4-chloro-phenyl]-1, 3-dihydro-1-methyl-2H-1,4-benzodiazepin-2-one (Ro 5-4864) or clonazepam was used. These data provide evidence for a relaxant effect of benzodiazepines on preconstricted renal vasculature.
Monasterolo
L A
LA
Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina.
Ochoa
J E
JE
Trumper
L
L
Elías
M M
MM
D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D001808:Blood Vessels / Q000187:drug effects*; D002998:Clonazepam / Q000494:pharmacology; D003292:Convulsants / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology*; D004231:Diuresis / Q000187:drug effects; D066298:In Vitro Techniques; D007677:Kidney Function Tests; D007684:Kidney Tubules / Q000187:drug effects*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D009318:Natriuresis / Q000187:drug effects; D009638:Norepinephrine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation / Q000187:drug effects
D001570:Benzodiazepinones; D003292:Convulsants; C028513:4'-chlorodiazepam; D002998:Clonazepam; D011189:Potassium Chloride; D003975:Diazepam; D009638:Norepinephrine