title: NMR, molecular modeling, and crystallographic studies of lentil lectin-sucrose interaction.
abstract: The conformational features of sucrose in the combining site of lentil lectin have been characterized through elucidation of a crystalline complex at 1.9-A resolution, transferred nuclear Overhauser effect experiments performed at 600 Mhz, and molecular modeling. In the crystal, the lentil lectin dimer binds one sucrose molecule per monomer. The locations of 229 water molecules have been identified. NMR experiments have provided 11 transferred NOEs. In parallel, the docking study and conformational analysis of sucrose in the combining site of lentil lectin indicate that three different conformations can be accommodated. Of these, the orientation with lowest energy is identical with the one observed in the crystalline complex and provides good agreement with the observed transferred NOEs. These structural investigations indicate that the bound sucrose has a unique conformation for the glycosidic linkage, close to the one observed in crystalline sucrose, whereas the fructofuranose ring remains relatively flexible and does not exhibit any strong interaction with the protein. Major differences in the hydrogen bonding network of sucrose are found. None of the two inter-residue hydrogen bonds in crystalline sucrose are conserved in the complex with the lectin. Instead, a water molecule bridges hydroxyl groups O2-g and O3-f of sucrose.
authors.affiliation: Institut National de la Recherche Agronomique, Nantes, France.
authors.forename: F
authors.initials: F
authors.lastname: Casset
authors.forename: T
authors.initials: T
authors.lastname: Hamelryck
authors.forename: R
authors.initials: R
authors.lastname: Loris
authors.forename: J R
authors.initials: JR
authors.lastname: Brisson
authors.forename: C
authors.initials: C
authors.lastname: Tellier
authors.forename: M H
authors.initials: MH
authors.lastname: Dao-Thi
authors.forename: L
authors.initials: L
authors.lastname: Wyns
authors.forename: F
authors.initials: F
authors.lastname: Poortmans
authors.forename: S
authors.initials: S
authors.lastname: Pérez
authors.forename: A
authors.initials: A
authors.lastname: Imberty
mesh_terms: D003198:Computer Simulation; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D037102:Lectins / Q000737:chemistry*; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008968:Molecular Conformation; D037121:Plant Lectins; D013395:Sucrose / Q000737:chemistry*
chemical_list: D037102:Lectins; D008024:Ligands; D037121:Plant Lectins; C033619:lentil lectin; D013395:Sucrose