title: The release of glutamate and accumulation of intracellular calcium in the guinea pig hippocampal slices during glucose deprivation.
abstract: Simultaneous recordings of the time course of change in the extracellular glutamate concentration ([Glu]O) and intracellular Ca2+ level [Ca2+]i during glucose deprivation were performed in the CA3 area of hippocampal slices from guinea pig. Direct measurement of [Glu]O with a glutamate sensitive sensor revealed that [Glu]0 showed a biphasic increase during glucose deprivation and decreased rapidly after re-introduction of glucose in the perfusing medium. The initial increase of [Glu]O preceded [Ca2+]i increase and thereafter a sustained increase of [Glu]O was observed concomitant with massive [Ca2+]i accumulation. Replacement of Na with Li markedly attenuated the rapid reduction of [Glu]O, suggesting that the reduction of [Glu]O was due to Na-dependent glutamate uptake system, which worked immediately after energy recovery.
authors.affiliation: Department of Physiology, Kobe University, School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Takata
authors.forename: H
authors.initials: H
authors.lastname: Hirai
authors.forename: T
authors.initials: T
authors.lastname: Shigemoto
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D005947:Glucose / Q000172:deficiency*; D018698:Glutamic Acid / Q000378:metabolism*; D006168:Guinea Pigs; D006624:Hippocampus / Q000378:metabolism*; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism*; D009994:Osmolar Concentration
chemical_list: D018698:Glutamic Acid; D005947:Glucose; D002118:Calcium