title: Endogenous marinobufagenin-like immunoreactive factor and Na+, K+ ATPase inhibition during voluntary hypoventilation.
abstract: In previous studies investigators found that conditioned hypoventilatory breathing potentiated a sodium-sensitive form of hypertension in dogs that was not mediated by sympathetic nervous system arousal. Our study investigated effects of 30 minutes of voluntary hypoventilation, maintained by a respiratory gas monitor and feedback procedure, in 16 normotensive humans of both sexes on (1) plasma concentrations of endogenous digitalis-like factors (ouabain-like and marinobufagenin-like immunoreactivity), (2) activity of erythrocyte Na+, K+ -ATPase, (3) inhibitory activity of plasma Na+, K+ -ATPase, and (4) blood pressure. Increased end tidal PCO2 (41 +/- 0.78 mm Hg versus 37.6 +/- 1.03 mm Hg) was associated with (1) an increase in plasma marinobufagenin-like immunoreactivity (1.23 +/- 0.47 versus 4.96 +/- 1.19 nmol/L), (2) an inhibition of Na+, K+ -ATPase in red blood cells (3.68 +/- 0.22 versus 2.15 +/- 0.25 mmol Pi/mL-1/h-1; P < .01), (3) increase in plasma Na+, K+ -ATPase inhibitory activity (34.9 +/- 4.0% versus 48.8 +/- 2.1%, P < .02), and (4) increases in systolic (112.4 +/- 2.6 versus 107.6 +/- 1.8 mm Hg) and diastolic (73.5 +/- 2.1 versus 68.8 +/- 2.1 mm Hg) blood pressures. Plasma levels of ouabain-like immunoreactivity did not increase significantly. Incubation of erythrocytes obtained during hypoventilation with antidigoxin antibody restored the Na+, K+ -ATPase activity (3.99 +/- 0.34 mmol Pi/mL-1/h-1). Cessation of hypoventilation was associated with decreases in diastolic blood pressure (70.5 +/- 2.2 mm Hg) and restoration of Na+, K+ -ATPase activity in erythrocytes (2.99 +/- 0.43 mmol Pi/mL-1/h1).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratory of Behavioral Sciences, National Institute on Aging, Baltimore, MD 21224, USA.
authors.forename: A Y
authors.initials: AY
authors.lastname: Bagrov
authors.forename: O V
authors.initials: OV
authors.lastname: Fedorova
authors.forename: J L
authors.initials: JL
authors.lastname: Austin-Lane
authors.forename: R I
authors.initials: RI
authors.lastname: Dmitrieva
authors.forename: D E
authors.initials: DE
authors.lastname: Anderson
mesh_terms: D000328:Adult; D001794:Blood Pressure; D002018:Bufanolides / Q000097:blood* / Q000652:urine; D004791:Enzyme Inhibitors / Q000097:blood; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D006801:Humans; D007040:Hypoventilation / Q000503:physiopathology*; D008297:Male; D010042:Ouabain / Q000097:blood / Q000652:urine; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis* / Q000037:antagonists & inhibitors
chemical_list: D002018:Bufanolides; D004791:Enzyme Inhibitors; C093896:marinobufagenin; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase