Optimisation of CCL64-based bioassay for TGF-beta.
Transforming growth factor beta (TGF-beta) is released by a variety of cells and known to be involved in many different processes including the immune response, wound healing and carcinogenesis. As most experimental investigations have been based on quantitative analysis of TGF-beta production using a bioassay, it seemed important to test the validity and limitations of this method. This paper analyses several parameters that may impair TGF-beta quantification by bioassay. Recommendations are made concerning the influence of technical parameters and the presence of other cytokines (EGF and bFGF) commonly released by cultured cells to which the Mv1Lu mink lung epithelial cell line (CCL64) is sensitive.
Garrigue-Antar
L
L
INSERM U 419, Institut de Biologie, Nantes, France.
Barbieux
I
I
Lieubeau
B
B
Boisteau
O
O
Grégoire
M
M
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001790:Blood Physiological Phenomena; D002277:Carcinoma / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D003110:Colonic Neoplasms / Q000378:metabolism; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004848:Epithelium / Q000187:drug effects / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006131:Growth Inhibitors / Q000032:analysis / Q000494:pharmacology; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008907:Mink; D011309:Preservation, Biological; D011994:Recombinant Proteins / Q000494:pharmacology; D012680:Sensitivity and Specificity; D013552:Swine; D016212:Transforming Growth Factor beta / Q000032:analysis* / Q000494:pharmacology
D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D016207:Cytokines; D006131:Growth Inhibitors; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor