Influence of DNA topology on expression of the tdc operon in Escherichia coli K-12.
TdcB activity expressed from the chromosomal gene and LacZ expression from single-copy tdc-lacZ transcriptional and translational fusions were measured in Escherichia coli strains harboring mutations in the genes encoding DNA gyrase, topoisomerase I and the HU protein. The pattern of tdc operon expression in these mutants suggests that relaxation of supercoiled DNA enhances tdc transcription in vivo.
Wu
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Y
Department of Biological Chemistry, The University of Michigan, Ann Arbor 48109-0606, USA.
Datta
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P
D004269:DNA, Bacterial / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D009690:Nucleic Acid Conformation; D009876:Operon
D004269:DNA, Bacterial