title: Localization of chromosome breakpoints induced by AluI and BamHI in Chinese hamster ovary cells treated in the G1 phase of the cell cycle.
abstract: Intact Chinese hamster ovary cells were exposed to the restriction endonucleases (REs) AluI or BamHI. In metaphase spreads from these cells, 300 breakpoints per RE were localized in G-banded chromosome type aberrations (dicentrics, translocations, rings, terminal and interstitial deletions). The majority of breakpoints induced by both REs were localized in G-light bands and showed a similar distribution of breakpoint clusters. RE digestion of metaphase spreads with AluI induced C-banding, and with BamHI G-banding. The data indicate that nuclease sensitive sites associated with active genes are mainly responsible for the distribution of breakpoints.
authors.affiliation: División de Citogenética Humana y Microscopía Cuantitativa, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
authors.forename: G A
authors.initials: GA
authors.lastname: Folle
authors.forename: G
authors.initials: G
authors.lastname: Obe
mesh_terms: D000818:Animals; D016466:CHO Cells; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D006224:Cricetinae; D015245:Deoxyribonuclease BamHI / Q000494:pharmacology*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000494:pharmacology*; D016193:G1 Phase
chemical_list: D015245:Deoxyribonuclease BamHI; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific