title: DNA damage in UVB-irradiated keratinocytes.
abstract: A DNA lesion which results from the breakdown of a pyrimidine base leaving a formamido remnant has been associated with oxidative stress. This lesion is shown to be produced in keratinocytes irradiated in culture with UVB light. The amount of formamido lesion produced is comparable to the amount of the 8-hydroxyguanine lesion. The two lesions were measured by 32P-postlabeling and electrochemical detection methods respectively.
authors.affiliation: Department of Experimental Therapeutics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
authors.forename: A E
authors.initials: AE
authors.lastname: Maccubbin
authors.forename: J
authors.initials: J
authors.lastname: Przybyszewski
authors.forename: M S
authors.initials: MS
authors.lastname: Evans
authors.forename: E E
authors.initials: EE
authors.lastname: Budzinski
authors.forename: H B
authors.initials: HB
authors.lastname: Patrzyc
authors.forename: M
authors.initials: M
authors.lastname: Kulesz-Martin
authors.forename: H C
authors.initials: HC
authors.lastname: Box
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000378:metabolism* / Q000528:radiation effects*; D004249:DNA Damage; D006147:Guanine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D015603:Keratinocytes / Q000378:metabolism* / Q000528:radiation effects*; D051379:Mice; D014466:Ultraviolet Rays
chemical_list: C024829:8-hydroxyguanine; D006147:Guanine; D004247:DNA