Molecular cloning of the complementary DNA for the mouse pyruvate kinase M-2 gene whose expression is dependent upon cell differentiation.
A cDNA encoding M2-type pyruvate kinase from mouse was cloned and its nucleotide sequence was determined. The cDNA encoded a protein containing 531 amino acids, the nucleotide and amino acid sequences of which were 93.2% and 98.1%, respectively, homologous to those of rat M2 pyruvate kinase. Expression of the pyruvate kinase in mouse embryonal carcinoma P19 cells altered according to differentiation stages; high at undifferentiated and low at differentiated stages.
Izumi
S
S
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Manabe
A
A
Tomoyasu
A
A
Kihara-Negishi
F
F
Ariga
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism*; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D011770:Pyruvate Kinase / Q000235:genetics*
D018076:DNA, Complementary; D011770:Pyruvate Kinase