Repression of preprotachykinin-A promoter activity is mediated by a proximal promoter element.
The rat preprotachykinin-A promoter, which is able to direct reporter gene expression in adult dorsal root ganglia neurons grown in culture, has no detectable activity in HeLa and PC12 cells. DNAase 1 footprinting and electrophoretic mobility shift analyses with HeLa nuclear extract indicated the presence of a protein complex binding to a region of the rat preprotachykinn-A gene promoter between the TATA box and the major transcriptional start site. We demonstrate that the sequence of the preprotachykinin-A promoter spanning nucleotides -47 to +92 functions to repress reporter gene expression in HeLa and PC12 cells but not in adult rat dorsal root ganglia grown in culture, and that this repression is correlated with a protein(s) binding to the element between the TATA box and major transcription initiation site. These results indicate that the tissue-specific expression of the preprotachykinin-A gene could require the interaction of both positive and negative regulatory DNA elements.
Mendelson
S C
SC
MRC Brain Metabolism Unit, Royal Edinburgh Hospital, U.K.
Morrison
C F
CF
McAllister
J
J
Paterson
J M
JM
Dobson
S P
SP
Mulderry
P K
PK
Quinn
J P
JP
D000818:Animals; D001665:Binding Sites; D002478:Cells, Cultured / Q000187:drug effects; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D016716:PC12 Cells / Q000187:drug effects; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D012097:Repressor Proteins; D013126:Spinal Nerve Roots; D015320:Tachykinins / Q000235:genetics* / Q000494:pharmacology; D014158:Transcription, Genetic
D012097:Repressor Proteins; D015320:Tachykinins