title: Antigenic determinants of the membrane-bound hydrogenase in Alcaligenes eutrophus are exposed toward the periplasm.
abstract: Electron microscopic immunogold labeling experiments were performed with ultrathin sections of plasmolyzed cells of Alcaligenes eutrophus and "whole-mount" samples of spheroplasts and protoplasts. They demonstrated that antigenic determinants of the membrane-bound hydrogenase are exposed, at the outside of the cytoplasmic membrane, to the periplasm.
authors.affiliation: Institut für Mikrobiologie, Georg-August-Universität zu Göttingen, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Eismann
authors.forename: K
authors.initials: K
authors.lastname: Mlejnek
authors.forename: D
authors.initials: D
authors.lastname: Zipprich
authors.forename: M
authors.initials: M
authors.lastname: Hoppert
authors.forename: H
authors.initials: H
authors.lastname: Gerberding
authors.forename: F
authors.initials: F
authors.lastname: Mayer
mesh_terms: D000421:Alcaligenes / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000276:immunology / Q000648:ultrastructure*; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure*; D016764:Cell Polarity; D000939:Epitopes; D006864:Hydrogenase / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure*; D016253:Microscopy, Immunoelectron; D011487:Protein Conformation
chemical_list: D001426:Bacterial Proteins; D000939:Epitopes; D008565:Membrane Proteins; D006864:Hydrogenase