title: Physical map of the linear chromosome of Streptomyces griseus.
abstract: The chromosomal DNA of Streptomyces griseus 2247 (a derivative of strain IFO3237) was digested with several restriction endonucleases and analyzed by pulsed-field gel electrophoresis (PFGE). Digestion with AseI and DraI gave 15 and 9 fragments, respectively, the total sizes of which were 7.8 Mb. All the AseI and DraI fragments were aligned on a linear chromosome map by using linking plasmids and cosmids. PFGE analysis of the intact chromosome also showed a linear DNA band of about 8 Mb. Detailed physical maps of both terminal regions were constructed; they revealed the presence of a 24-kb terminal inverted repeat on each end. PFGE analysis with and without proteinase K treatment suggested that each end of the chromosome carries a protein molecule.
authors.affiliation: Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Lezhava
authors.forename: T
authors.initials: T
authors.lastname: Mizukami
authors.forename: T
authors.initials: T
authors.lastname: Kajitani
authors.forename: D
authors.initials: D
authors.lastname: Kameoka
authors.forename: M
authors.initials: M
authors.lastname: Redenbach
authors.forename: H
authors.initials: H
authors.lastname: Shinkawa
authors.forename: O
authors.initials: O
authors.lastname: Nimi
authors.forename: H
authors.initials: H
authors.lastname: Kinashi
mesh_terms: D002874:Chromosome Mapping / Q000379:methods; D002876:Chromosomes, Bacterial; D003360:Cosmids / Q000235:genetics; D015342:DNA Probes; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D016521:Electrophoresis, Gel, Pulsed-Field; D008970:Molecular Weight; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013305:Streptomyces griseus / Q000235:genetics*; D016615:Telomere / Q000235:genetics
chemical_list: D015342:DNA Probes; D004269:DNA, Bacterial; C052712:endodeoxyribonuclease VspI; D015252:Deoxyribonucleases, Type II Site-Specific; C035594:TTTAAA -specific type II deoxyribonucleases