On the cellular basis of immunological T cell memory.
We have studied memory in T cell receptor (TCR) transgenic mice expressing a Db-restricted TCR specific for the male peptide (H-Y). CD8+ T cells from female TCR transgenic C57BL/6 (B6) mice were activated by transferring them into X-irradiated male (B6 x bm12)F1 hybrid recipients. Subsequently, they were highly purified by cell sorting and transferred for various lengths of time into female B6 nu/nu recipient mice. Other nu/nu recipient mice received highly purified naive T cells expressing the transgenic TCR. The functional potential of naive and "memory" T cells was analyzed by stimulation with male cells in vivo. The results show that memory cells can be derived from activated T cells and persist in the absence of antigen for at least 13 weeks. Naive and memory T cells differ in that memory T cells give a more vigorous and sustained response than naive T cells.
Bruno
L
L
Basel Institute for Immunology, Switzerland.
Kirberg
J
J
von Boehmer
H
H
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D005260:Female; D006183:H-2 Antigens / Q000276:immunology*; D006182:H-Y Antigen / Q000276:immunology*; D064532:Histocompatibility Antigen H-2D; D007114:Immunization; D007156:Immunologic Memory / Q000502:physiology*; D016219:Immunotherapy, Adoptive; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008822:Mice, Transgenic; D011828:Radiation Chimera; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*
D006183:H-2 Antigens; D006182:H-Y Antigen; D064532:Histocompatibility Antigen H-2D; D011948:Receptors, Antigen, T-Cell