The synthesis of melanin-concentrating hormone is stimulated by ventromedial hypothalamic lesions in the rat lateral hypothalamus: a time-course study.
The activity of melanin-concentrating (MCH) neurons, was investigated by immunocytochemical and hybridocytochemical techniques in male rats bearing limited lesions of the ventromedial hypothalamic nuclei (VMN). 2 days after operation, the abundance of immunoreactive cell bodies and fibres and the intensity of labelling seemed slightly decreased in lesioned rats as compared to controls while no significant difference could be detected in MCH gene expression. After 8 days, synthesis, storage and transport of MCH appeared strongly stimulated and this stimulation lasted until the end of the experiment (day 35), suggesting that VMN plays a physiological role in controlling MCH neuron activity.
Griffond
B
B
C.N.R.S. URA 561, Laboratoire d'Histologie Embryologie Cytogénétique, Faculté de Médecine, Besançon, France.
Deray
A
A
Nguyen
N U
NU
Colard
C
C
Fellmann
D
D
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D004435:Eating; D007026:Hypothalamic Area, Lateral / Q000166:cytology / Q000378:metabolism*; D007028:Hypothalamic Hormones / Q000096:biosynthesis*; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007328:Insulin / Q000097:blood; D008297:Male; D008543:Melanins / Q000096:biosynthesis*; D009474:Neurons / Q000378:metabolism*; D010907:Pituitary Hormones / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013238:Stereotaxic Techniques; D013997:Time Factors; D014697:Ventromedial Hypothalamic Nucleus / Q000502:physiology* / Q000601:surgery
D001786:Blood Glucose; D007028:Hypothalamic Hormones; D007328:Insulin; D008543:Melanins; D010907:Pituitary Hormones; D012333:RNA, Messenger; C019463:melanin-concentrating hormone