Histochemical studies of progressive p53 mutations during colonic carcinogenesis in Sprague-Dawley rats induced by N-methyl-N-nitro-nitrosoguanidine or azoxymethane.
We studied the increasing expression of the p53 tumor suppressor gene in Sprague-Dawley rats, chemically induced to develop colon cancer. p53 expression was evaluated histochemically at various stages of tumor progression (during a period of 40 weeks) that can be followed by colonic hyperproliferation labeled by 3H-thymidine incorporation. We found that high level nuclear expression of p53 protein correlates with progression of malignancy in carcinogen-induced animals, whereas cytoplasmic staining is related to the onset and early development of malignancy.
Tendler
Y
Y
Clinical and Research Laboratories, Nahariya Regional Hospital, Israel.
Reshef
R
R
Cohen
I
I
Barzilai
M
M
Shasha
S M
SM
Rotter
V
V
Shkolnik
T
T
D000230:Adenocarcinoma / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D000818:Animals; D001397:Azoxymethane; D002467:Cell Nucleus / Q000737:chemistry; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000473:pathology; D003593:Cytoplasm / Q000737:chemistry; D004848:Epithelium / Q000473:pathology; D015870:Gene Expression; D016158:Genes, p53; D007150:Immunohistochemistry; D008297:Male; D008769:Methylnitronitrosoguanidine; D009154:Mutation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016159:Tumor Suppressor Protein p53 / Q000032:analysis*
D016159:Tumor Suppressor Protein p53; D008769:Methylnitronitrosoguanidine; D001397:Azoxymethane