[Generation of active forms of oxygen by antibiotics of the tetracycline series during tetracycline catalysis of oxidation of ferrous iron].
During oxidation of Fe(2+) catalyzed by tetracyclines there was recorded lucigenin, activated chemiluminescence evident of generation of the oxygen radicals. It was also observed that during the Fe(2+) oxidation by the molecular oxygen catalyzed by tetracyclines there generated hydrogen peroxide which accelerated the Fe(2+) oxidation recorded photometrically by the formation of strongly absorbing tetracycline complexes with Fe(2+). In the presence of ascorbate reducing Fe(2+) in the complexes with tetracyclines and their subsequent oxidation there generated radicals modifying the antibiotic molecules evident from a change in their absorption spectra after the respective incubation. The results offered a pattern describing the mechanism of the tetracycline toxic effect on biological objects.
Petrenko
I U
IU
Titov
V Iu
VIu
Vladimirov
Iu A
IuA
D000166:Acridines; D001205:Ascorbic Acid / Q000378:metabolism; D002384:Catalysis; D005296:Ferrous Compounds / Q000378:metabolism*; D008163:Luminescent Measurements; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013053:Spectrophotometry; D013754:Tetracyclines / Q000494:pharmacology*
D000166:Acridines; D005296:Ferrous Compounds; D017382:Reactive Oxygen Species; D013754:Tetracyclines; C033472:10,10'-dimethyl-9,9'-biacridinium; D001205:Ascorbic Acid