Comparative mapping of YRRM- and TSPY-related cosmids in man and hominoid apes.
Using chromosomal in situ hybridization it has been demonstrated that specific members of the YRRM and the TSPY families are multicopy and Y chromosome specific in hominoids. After hybridization with the YRRM-related cosmid A5F and the TSPY-related cosmids cos36 and cY91, a reverse and complementary pattern of main and secondary signals is detected on the Y chromosomes of the human, the pygmy chimpanzee and the gorilla, while the location of signals coincides on the Y chromosomes of the chimpanzee, both orang-utan subspecies and the white hand gibbon. This complementary distribution of YRRM and TSPY sequences on the hominoid Y chromosomes possibly originates from a similar sequence motif that is shared by and evolutionarily conserved between certain members of both gene families and/or repeated elements flanking those genes. Otherwise this complementary distribution could go back to a common organization of these genes next to each other on an ancient Y chromosome which was disrupted by chromosomal rearrangements and amplification of one or other of the genes at each of the locations.
Schempp
W
W
Institute of Human Genetics and Anthropology, University of Freiburg, Germany.
Binkele
A
A
Arnemann
J
J
Gläser
B
B
Ma
K
K
Taylor
K
K
Toder
R
R
Wolfe
J
J
Zeitler
S
S
Chandley
A C
AC
D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D017124:Conserved Sequence; D003360:Cosmids / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D006922:Hylobates / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D008297:Male; D009687:Nuclear Proteins; D012733:Sex Differentiation / Q000235:genetics*; D051876:Sex-Determining Region Y Protein; D013091:Spermatogenesis / Q000235:genetics*; D014157:Transcription Factors; D014998:Y Chromosome
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; C497749:TSPY1 protein, human; D014157:Transcription Factors