Interleukin 8 and biocompatibility of dialysis membranes.
To determine whether interleukin 8 (IL-8) can be used as an index of biocompatibility of dialysis membranes, the effects of hemodialysis (HD) on plasma IL-8 levels and the expression of IL-8 mRNA in peripheral blood mononuclear cell (PBMC) were compared among regenerated cellulose (RC), polyacrylonitrile (PAN) and polymethylmethacrylate (PMMA) dialyzers. HD using RC dialysers significantly increased plasma IL-8 levels and induced abundant expression of IL-8 mRNA in PBMC. HD using PMMA dialyzers also increased plasma IL-8 levels and induced slight expression of IL-8 mRNA. In contrast, HD using PAN dialyzers neither increased plasma IL-8 levels nor induced the expression of IL-8 mRNA. In vitro studies demonstrated that IL-8 was considerably adsorbed by PAN membranes and not at all by RC and PMMA. These studies indicate that plasma IL-8 level and expression of PBMC IL-8 mRNA can be used as indices of biocompatibility of dialysis membranes.
Niwa
T
T
Department of Internal Medicine, Nagoya University Branch Hospital, Japan.
Miyazaki
T
T
Sato
M
M
Kambe
F
F
Tsuzuki
T
T
Uema
K
K
Maeda
K
K
Seo
H
H
D000180:Acrylic Resins; D001672:Biocompatible Materials; D002482:Cellulose; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000097:blood; D007676:Kidney Failure, Chronic / Q000097:blood / Q000628:therapy; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D006435:Renal Dialysis / Q000295:instrumentation*
D000180:Acrylic Resins; D001672:Biocompatible Materials; D016209:Interleukin-8; D008567:Membranes, Artificial; D008768:Methylmethacrylates; D012333:RNA, Messenger; C010504:polyacrylonitrile; D002482:Cellulose