Trophic actions of 2-chloroadenosine and bFGF on cultured myenteric neurones.
The effects of the stable adenosine analogue, 2-chloroadenosine (2-CA) and basic fibroblast growth factor (bFGF) on myenteric neurones in dissociated cell culture were examined. 2-CA had no effect on neuronal numbers, but increased neurite length, in a dose-dependent manner. bFGF increased both the number of myenteric neurones and neurite length. When 2-CA was applied together with bFGF, an enhanced increase in neurite outgrowth, but no additional increase in neuronal numbers was observed. 2-CA-induced effects were blocked by the adenosine antagonist 8-(p-sulphophenyl)theophylline. These results show, for the first time, that both purines and bFGF may have trophic actions on myenteric neurones and also indicate that purines enhance some effects of bFGF, in a synergistic manner.
Schäfer
K H
KH
Department of Anatomy and Developmental Biology, University College London, UK.
Saffrey
M J
MJ
Burnstock
G
G
D015762:2-Chloroadenosine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D004357:Drug Synergism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D005724:Ganglia / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D009197:Myenteric Plexus / Q000166:cytology* / Q000187:drug effects; D016501:Neurites / Q000502:physiology / Q000648:ultrastructure; D009474:Neurons / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley
D016222:Fibroblast Growth Factor 2; D015762:2-Chloroadenosine