title: Vascular and tubular actions of diazepam in isolated and perfused rat kidney.
abstract: The effects of diazepam were studied in the isolated rat kidney under conditions of constant flow. Kidneys were perfused with modified Ringer-Krebs solution. Diazepam produced a raised fractional excretion of water and sodium without hemodynamic changes, suggesting a direct effect on tubular transport mechanisms. Diazepam decreased renal perfusion pressure in a concentration-dependent fashion when kidneys were pretreated with either noradrenaline or potassium chloride. Similar responses were observed when 7-chloro-5-[4-chloro-phenyl]-1, 3-dihydro-1-methyl-2H-1,4-benzodiazepin-2-one (Ro 5-4864) or clonazepam was used. These data provide evidence for a relaxant effect of benzodiazepines on preconstricted renal vasculature.
authors.affiliation: Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina.
authors.forename: L A
authors.initials: LA
authors.lastname: Monasterolo
authors.forename: J E
authors.initials: JE
authors.lastname: Ochoa
authors.forename: L
authors.initials: L
authors.lastname: Trumper
authors.forename: M M
authors.initials: MM
authors.lastname: Elías
mesh_terms: D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D001693:Biological Transport, Active / Q000187:drug effects; D001808:Blood Vessels / Q000187:drug effects*; D002998:Clonazepam / Q000494:pharmacology; D003292:Convulsants / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology*; D004231:Diuresis / Q000187:drug effects; D066298:In Vitro Techniques; D007677:Kidney Function Tests; D007684:Kidney Tubules / Q000187:drug effects*; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D009318:Natriuresis / Q000187:drug effects; D009638:Norepinephrine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012079:Renal Circulation / Q000187:drug effects
chemical_list: D001570:Benzodiazepinones; D003292:Convulsants; C028513:4'-chlorodiazepam; D002998:Clonazepam; D011189:Potassium Chloride; D003975:Diazepam; D009638:Norepinephrine