title: Purification and characterization of an NAD(P)H:quinone oxidoreductase from Glycine max seedlings.
abstract: An NAD(P)H:(quinone acceptor) oxidoreductase (EC 1.6.99.2) was purified from Glycine max seedlings by means of chromatographic procedures. After 1371-fold purification, the enzyme showed a single band in IEF corresponding to an isoelectric point of 6.1. A single band was also found in native-PAGE both by activity staining and Coomassie brilliant blue staining. The molecular mass determined in SDS-PAGE was 21900 Da, while in HPLC gel-filtration it was 61000 Da. The NAD(P)H:quinone oxidoreductase was able to use NADH or NADPH as the electron donor. Among the artificial quinones which are reduced by this enzyme, 6-hydroxydopa- and 6-hydroxydopamine-quinone are of particular interest because of their neurotoxic effects.
authors.affiliation: Istituto di Chimica Biologica, Università di Cagliari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Rescigno
authors.forename: F
authors.initials: F
authors.lastname: Sollai
authors.forename: S
authors.initials: S
authors.lastname: Masala
authors.forename: M C
authors.initials: MC
authors.lastname: Porcu
authors.forename: E
authors.initials: E
authors.lastname: Sanjust
authors.forename: A C
authors.initials: AC
authors.lastname: Rinaldi
authors.forename: N
authors.initials: N
authors.lastname: Curreli
authors.forename: D
authors.initials: D
authors.lastname: Grifi
authors.forename: A
authors.initials: A
authors.lastname: Rinaldi
mesh_terms: D015086:2,6-Dichloroindophenol / Q000378:metabolism; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010944:Plants / Q000201:enzymology; D013025:Glycine max / Q000201:enzymology*; D013053:Spectrophotometry; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D009243:NAD; D009249:NADP; D015086:2,6-Dichloroindophenol; D016660:NAD(P)H Dehydrogenase (Quinone)