title: Negative-ion fast atom bombardment tandem mass spectrometry for characterization of sulfated unsaturated disaccharides from heparin and heparan sulfate.
abstract: Negative-ion fast atom bombardment tandem mass spectrometry has been used in the characterization of non-, mono-, di- and trisulfated disaccharides from heparin and heparan sulfate. The positional isomers of the sulfate group of monosulfated disaccharides were distinguished from each other by negative-ion fast atom bombardment tandem mass spectra, which provide an easy way of identifying the positional isomers. This fast atom bombardment collision induced dissociation mass spectrometry/mass spectrometry technique was also applied successfully to the characterization of di- and trisulfated disaccharides.
authors.affiliation: Research and Development Laboratories, Soda Aromatic Co., Ltd, Chiba, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ii
authors.forename: M
authors.initials: M
authors.lastname: Kubota
authors.forename: S
authors.initials: S
authors.lastname: Okuda
authors.forename: T
authors.initials: T
authors.lastname: Hirano
authors.forename: M
authors.initials: M
authors.lastname: Ohashi
mesh_terms: D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000032:analysis* / Q000737:chemistry; D006493:Heparin / Q000737:chemistry*; D006497:Heparan Sulfate / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods; D013431:Sulfates / Q000032:analysis* / Q000737:chemistry
chemical_list: D004187:Disaccharides; D006493:Heparin; D006497:Heparan Sulfate; D013431:Sulfates