The T-cell receptor V beta repertoire in naive and human immunodeficiency virus-1 (HIV-1) infected chimpanzees.
Using a panel of human T-cell receptor (TCR) variable region beta chain (V beta) polymerase chain reaction (PCR) primers, we performed cross-sectional and longitudinal analyses of the TCR V beta repertoire in naive and HIV-1 infected chimpanzees. We demonstrate that our TCR PCR primer panel will support amplification of chimpanzee cDNA from most of the TCR V beta families. However, no differences in TCR V beta expression were found between the naive and HIV-1 infected chimpanzees, unlike the TCR V beta repertoire perturbation found in HIV-1 infected human subjects. This finding suggests that a complete TCR repertoire in HIV-1 infected chimpanzees is associated with the maintenance CD4+ T-cell numbers and lack of progression to AIDS.
Boldt-Houle
D M
DM
Department of Pathology, University of Pittsburgh School of Medicine, PA 15261, USA.
Nigida
S M
SM
Rinaldo
C R
CR
Ehrlich
G D
GD
D000818:Animals; D001483:Base Sequence; D003430:Cross-Sectional Studies; D004195:Disease Models, Animal; D005260:Female; D015658:HIV Infections / Q000235:genetics / Q000276:immunology*; D015497:HIV-1; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D008969:Molecular Sequence Data; D002679:Pan troglodytes / Q000276:immunology*; D016133:Polymerase Chain Reaction; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis / Q000235:genetics*
D016693:Receptors, Antigen, T-Cell, alpha-beta