Inhibition of apoptosis by the expression of antisense Nedd2.
Nedd2 belongs to a family of mammalian cysteine proteases which share similarity with the Caenorhabditis elegans cell death protein, Ced-3. Overexpression of Nedd2 has been shown to induce apoptosis in mammalian cells but in the absence of a specific known inhibitor, it remains to be seen whether this represents cytotoxic effects of the Nedd2 protease or the specific activation of an apoptotic pathway. The present work shows that the factor-dependent cell line FDC-P1 expressing mouse antisense Nedd2 mRNA, exhibits significant inhibition of cell death upon removal of the cytokines, thus providing evidence for a direct role of Nedd2 in mediating apoptosis.
Kumar
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Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Adelaide, Australia.
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D053143:Caspase 2; D002460:Cell Line; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D006801:Humans; D051379:Mice; D016372:RNA, Antisense / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
D015853:Cysteine Proteinase Inhibitors; D016372:RNA, Antisense; D012333:RNA, Messenger; C490360:CASP2 protein, human; C090208:Casp2 protein, mouse; D053143:Caspase 2; D003546:Cysteine Endopeptidases