title: Effects of dietary fat supplementation on inositol phosphate release and metabolism in rat left atria.
abstract: Eight weeks dietary supplementation with oils enriched in saturated fats, n-6 polyunsaturated fats and n-3 polyunsaturated fats resulted in a reduced inositol phosphate response in isolated rat left atria. Reductions in both basal activity and norepinephrine-stimulated activity were observed. Diets supplemented with n-3 polyunsaturated fats produced a greater decrease in the norepinephrine-stimulated release than the other dietary groups. In addition, supplementation with n-6 polyunsaturated fats resulted in higher levels of the Ca(2+)-releasing compound inositol(1,4,5)trisphosphate while addition of n-3 fats eliminated accumulation of inositol(1, 4)bisphosphate in response to norepinephrine. The reduction in inositol phosphate accumulation observed in all fat-supplemented groups demonstrates the need for caution in choosing relevant control groups in such dietary studies. The specific effects of n-6 and n-3 polyunsaturated fats on individual inositol phosphate isomers demonstrates subtle effects on inositol phosphate metabolism, the significance of which requires further investigation.
authors.affiliation: Cellular Biochemistry Laboratory, Baker Medical Research Institute, Melbourne, Australia.
authors.forename: E A
authors.initials: EA
authors.lastname: Woodcock
authors.forename: K E
authors.initials: KE
authors.lastname: Anderson
authors.forename: X J
authors.initials: XJ
authors.lastname: Du
authors.forename: A M
authors.initials: AM
authors.lastname: Dart
mesh_terms: D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage*; D006325:Heart Atria / Q000187:drug effects / Q000378:metabolism*; D007295:Inositol Phosphates / Q000378:metabolism*; D008297:Male; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004041:Dietary Fats; D007295:Inositol Phosphates; D009638:Norepinephrine