Effects of cisplatin and selenite on the level of thiols in pig blood platelets.
The level of free sulfhydryl groups of glutathione and proteins in pig blood platelets after short lasting incubation (30 min.) of these cells with cisplatin (cis-diamminedichloroplatinum II) and sodium selenite was investigated in vitro. The concentration of the platelet thiols after sodium selenite or cisplatin treatment distinctly decreased (p < 0.001). Pretreatment of blood platelets with sodium selenite at the low concentration (0.1 microM) protected platelet glutathione against the reaction with cisplatin (p < 0.001). The present study shows that in blood platelets after the incubation with cisplatin the GS-platinum complex was formed. The results of our experiments demonstrated also that the extracellular GSH (1 mM) had the protective effect against cisplatin-induced peroxidation of platelet lipids.
Wachowicz
B
B
Department of Biochemistry, University of Lódź, Poland.
Krajewski
T
T
Olas
B
B
Zbikowska
H M
HM
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002945:Cisplatin / Q000494:pharmacology*; D008315:Malondialdehyde / Q000037:antagonists & inhibitors / Q000097:blood; D018038:Sodium Selenite / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000097:blood*; D013552:Swine / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D018038:Sodium Selenite; D002945:Cisplatin