title: Cloned antigens and antiidiotypes.
abstract: Both monoclonal and polyclonal antiidiotypic antibodies mimicking the human colorectal carcinoma (CRC) associated antigen CO17-1A/GA733 have induced antigen-specific humoral and cellular immunity in CRC patients. The immune responses may underlie the clinical responses observed in some of the treated patients. Recently, the CO17-1A/GA733 antigen has been molecularly cloned and expressed in baculo-, adeno-, and vaccinia viruses. In preclinical studies, these recombinant antigen preparations elicited specific humoral immunity (cytotoxic antibodies) and cellular immunity (DTH-reactive and proliferative T cells). Antibody titers elicited in animals by recombinant antigen were significantly higher than those elicited by antiidiotypes. The recombinant antigen has a potential as a vaccine for CRC patients.
authors.affiliation: Wistar Institute, Philadelphia, PA 19104, USA.
authors.forename: D
authors.initials: D
authors.lastname: Herlyn
authors.forename: R
authors.initials: R
authors.lastname: Somasundaram
authors.forename: J
authors.initials: J
authors.lastname: Zaloudik
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: L
authors.initials: L
authors.lastname: Jacob
authors.forename: D
authors.initials: D
authors.lastname: Harris
authors.forename: M P
authors.initials: MP
authors.lastname: Kieny
authors.forename: R
authors.initials: R
authors.lastname: Ricciardi
authors.forename: E
authors.initials: E
authors.lastname: Gonczol
authors.forename: H
authors.initials: H
authors.lastname: Sears
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000235:genetics*; D000912:Antibodies, Neoplasm / Q000096:biosynthesis; D000917:Antibody Formation; D000951:Antigens, Neoplasm / Q000276:immunology* / Q000627:therapeutic use; D002277:Carcinoma / Q000235:genetics* / Q000276:immunology / Q000628:therapy; D003001:Cloning, Molecular; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000276:immunology / Q000628:therapy; D006801:Humans; D007111:Immunity, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology* / Q000627:therapeutic use
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D011994:Recombinant Proteins