Antigenic determinants of the membrane-bound hydrogenase in Alcaligenes eutrophus are exposed toward the periplasm.
Electron microscopic immunogold labeling experiments were performed with ultrathin sections of plasmolyzed cells of Alcaligenes eutrophus and "whole-mount" samples of spheroplasts and protoplasts. They demonstrated that antigenic determinants of the membrane-bound hydrogenase are exposed, at the outside of the cytoplasmic membrane, to the periplasm.
Eismann
K
K
Institut für Mikrobiologie, Georg-August-Universität zu Göttingen, Germany.
Mlejnek
K
K
Zipprich
D
D
Hoppert
M
M
Gerberding
H
H
Mayer
F
F
D000421:Alcaligenes / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000276:immunology / Q000648:ultrastructure*; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology / Q000648:ultrastructure*; D016764:Cell Polarity; D000939:Epitopes; D006864:Hydrogenase / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure*; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000276:immunology / Q000302:isolation & purification / Q000648:ultrastructure*; D016253:Microscopy, Immunoelectron; D011487:Protein Conformation
D001426:Bacterial Proteins; D000939:Epitopes; D008565:Membrane Proteins; D006864:Hydrogenase