title: On the nature of replication origins in higher eukaryotes.
abstract: Establishing whether DNA replication in higher eukaryotic cells is regulated by genetic replicators has been one of the more challenging problems in cell biology. Several important replicon-mapping techniques have been developed in the past decade that have opened up new windows on replication origins. In the past few years, the application of these strategies has identified a large number of origins in a variety of different loci and organisms. Comparison of sequence motifs and chromosomal milieu, as well as genetic manipulation, should begin to uncover the secrets of these illusive regulatory elements.
authors.affiliation: Department of Biochemistry, University of Virginia School of Medicine, Charlottesville 22908, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Hamlin
authors.forename: P A
authors.initials: PA
authors.lastname: Dijkwel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005057:Eukaryotic Cells; D006801:Humans; D008969:Molecular Sequence Data; D018741:Replication Origin; D012093:Replicon