Unchanged 5'-deiodinating activity during the induction of a nonthyroidal illness.
We investigated the formation of a "nonthyroidal illness" (NTI) in pigs undergoing ventricular fibrillation (VF) and resuscitation. Seven minutes after VF twenty-one pigs received either Epinephrine (E: 45 micrograms/kg B.W.; n = 7), Norepinephrine (NE: 45 micrograms/kg B.W.; n = 7), or Vasopressin (VP: 0.8 U/kg B.W.; n = 7). We determined the serum concentrations (sc) of total T4 (TT4), FT4, total T3 (TT3) and rT3 120 min before, during (t0), and 5, 15, 60 and 120 min after VF. At the end of the observation period we figured out the in-vitro T3-generation (kM, Vmax), the in-vitro rT3-generation, the in-vitro rT3-decomposition (kM, Vmax) and the content of cytosolic sulfhydryls (total sulfhydryls, non-protein bound sulfhydryls) in liver and kidney specimen. Animals not undergoing VF served as controls (C) for parameters measured in the intracellular compartment. TT4- and TT3-sc decreased to 3.3 +/- 0.6 micrograms/dl (p < 0.05, vs. t0) and 15.2 +/- 4.1 ng/dl (p < 0.05, vs t0), resp. FT4-sc remained stable for five minutes (2.63 +/- 0.41 ng/dl) before declining to 1.8 +/- 0.39 ng/dl (p < 0.05, vs. t0). The rT3-sc raised finally to 46.9 +/- 7.3 ng/dl (p < 0.05, vs t0). Iodothyronine sc did not exhibit differences between E-, NE- and VP-treatment. Neither in-vitro T3-generation, nor in-vitro rT3-generation, nor in-vitro rT3-decomposition nor intracellular sulfhydryl content were affected by the events of VF and resuscitation as compared to the controls.(ABSTRACT TRUNCATED AT 250 WORDS)
Wolf
C F
CF
Institut für Klinische Chemie, Medizinische Universitätsklinik und Poliklinik, Universität Ulm, Germany.
Keck
F S
FS
Brinkmann
A
A
Rigos
D
D
Lindner
K H
KH
Grünert
A
A
D000818:Animals; D004837:Epinephrine / Q000494:pharmacology; D007453:Iodide Peroxidase / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology; D009638:Norepinephrine / Q000494:pharmacology; D012151:Resuscitation; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013552:Swine; D013974:Thyroxine / Q000378:metabolism; D014284:Triiodothyronine / Q000096:biosynthesis; D014667:Vasopressins / Q000494:pharmacology; D014693:Ventricular Fibrillation / Q000201:enzymology*
D013438:Sulfhydryl Compounds; D014284:Triiodothyronine; D014667:Vasopressins; D007453:Iodide Peroxidase; D013974:Thyroxine; D009638:Norepinephrine; D004837:Epinephrine