A developmentally regulated chromosomal origin of replication uses essential transcription elements.
Only one of the two chromosomes in the asymmetric Caulobacter predivisional cell initiates replication in the progeny cells. Transcription from a strong promoter within the origin occurs uniquely from the replication-competent chromosome at the stalked pole of the predivisional cell. This regulated promoter has an unusual sequence organization, and transcription from this promoter is essential for regulated (cell type-specific) replication. Our analysis defines a new class of bacterial origins and suggests a coupling between transcription and replication that is consistent with the phylogenetic relationship of Caulobacter to the ancestral mitochondrion.
Marczynski
G T
GT
Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, California 94305-5427, USA.
Lentine
K
K
Shapiro
L
L
D001483:Base Sequence; D016934:Caulobacter / Q000235:genetics*; D002455:Cell Division; D002876:Chromosomes, Bacterial; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D018507:Gene Expression Regulation, Developmental; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D018741:Replication Origin; D017384:Sequence Deletion; D014158:Transcription, Genetic