Decline of taste sensitivity in protein deficient adult rats.
The influence of dietary protein levels on taste sensitivity was studied in adult rats. Low protein diets of 0.0, 2.5, or 5.0% purified egg protein (PEP) were fed to animals for 28 days. Two bottle choice preference tests between aqueous solutions of either 2, 9, 17, or 86 mM sodium chloride and deionized water were conducted in an ascending order on days 14, 16, 18, and 20. Urine samples were collected for zinc and creatinine analysis. Blood samples were also collected for measuring serum zinc and creatinine concentrations. Scanning electron microscopy was performed to observe rats' tongue epithelia. Protein free diet group showed significantly lower taste sensitivity and renal reabsorption rate than other protein containing diet groups, while serum zinc and creatinine concentrations, and creatinine clearance were not affected by dietary protein level. Degeneration of filiform papillae and imperforation of taste pore of fungiform papillae were observed in protein free diet group. This experiment implies at least 2.5% dietary protein is required to manifest normal taste function in the adult.
Ohara
I
I
Laboratory of Nutrition, Faculty of Home Economics, Kobe Women's University, Japan.
Tabuchi
R
R
Kimura
M
M
Itokawa
Y
Y
D000367:Age Factors; D000818:Animals; D003401:Creatine / Q000097:blood; D005260:Female; D005518:Food Preferences / Q000502:physiology; D005919:Glomerular Filtration Rate / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D009410:Nerve Degeneration / Q000502:physiology; D011488:Protein Deficiency / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013650:Taste Buds / Q000473:pathology / Q000503:physiopathology; D013652:Taste Threshold / Q000502:physiology*; D015032:Zinc / Q000097:blood
D015032:Zinc; D003401:Creatine