Lewis X structure increases cell substratum adhesion in L cells.
cDNAs of alpha-1,3-fucosyltransferase as well as alpha-1,3/4-fucosyltransferase were placed under the control of a beta-actin promoter and cytomegalovirus enhancer and were introduced into L cells. The transfected cells expressing Le(x) antigen showed increased cell substratum adhesion as compared to the antigen-negative cells, when they were cultured for 2 to 4 h in Dulbecco-modified minimum essential medium containing 0.05% bovine serum albumin. The increased cell substratum adhesion was completely inhibited by cycloheximide and anti-integrin antiserum, and partly by an RGD peptide and EGTA. These findings indicate that Le(x) structure promotes cell adhesion to substratum-bound material secreted by cells, and that the increased adhesion is mediated by integrin. Western blotting experiments have revealed an 85 kDa protein and a 50-60 kDa protein as carriers of Le(x) antigen in transfected cells. The latter is likely to be basigin, which is a member of the immunoglobulin superfamily and is considered to be an integrin-associated protein. We hypothesize that fucosylation of basigin enhances integrin-mediated cell substratum adhesion.
Sudou
A
A
Department of Biochemistry, Nagoya University School of Medicine.
Ozawa
M
M
Muramatsu
T
T
D000818:Animals; D002240:Carbohydrate Sequence; D018236:Carcinoma, Embryonal; D002448:Cell Adhesion / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D018076:DNA, Complementary; D004492:Edetic Acid / Q000494:pharmacology; D005647:Fucosyltransferases / Q000096:biosynthesis / Q000378:metabolism*; D006801:Humans; D007739:L Cells; D016256:Lewis X Antigen / Q000096:biosynthesis / Q000302:isolation & purification / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity; D014162:Transfection; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D016256:Lewis X Antigen; D009842:Oligopeptides; D011994:Recombinant Proteins; D003513:Cycloheximide; D004492:Edetic Acid; D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase; C019818:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase