title: Two kinds of neutral serine proteinases in salted muscle of anchovy, Engraulis japonica.
abstract: Two kinds of proteinases, type-I and type-II, were purified or partially purified from salted muscle of anchovy, Engraulis japonica. Mol. wts. of type-I and type-II proteinases were estimated to 25,000 and 37,000, respectively, on electrophoretic analysis. Both proteinases strongly hydrolyzed synthetic tri or tetrapeptide substrates specific to trypsin, alpha-thrombin, and an activated protein C, while they hardly hydrolyzed Arg-MCA and benzoyl Arg-MCA derivatives. The proteinases were inhibited by common trypsin inhibitors. Optimal pH for the proteinase activities were pH 6.8 (type-I) and pH 7.0 to 7.5 (type-II), and the proteinases showed the highest activities at 45 degrees C (type-I) and 50 degrees C (type-II). The N-terminal amino acid sequence of type-I proteinase, 1I-2V-3G-4G ... (29 residues were identified), was significantly similar to sequences of trypsins and tryptases. Based on these findings, both proteinases were presumed to be kinds of tryptases in E. japonica muscle.
authors.affiliation: Laboratory of Biochemistry of Marine Resources, Tokyo University of Fisheries, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ishida
authors.forename: N
authors.initials: N
authors.lastname: Sugiyama
authors.forename: M
authors.initials: M
authors.lastname: Sato
authors.forename: F
authors.initials: F
authors.lastname: Nagayama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004795:Enzyme Stability; D005399:Fishes; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008969:Molecular Sequence Data; D009132:Muscles / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology; D012965:Sodium Chloride; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D015842:Serine Proteinase Inhibitors; D012965:Sodium Chloride; D012697:Serine Endopeptidases