Implication of the alpha v beta 3 integrin in the adhesion of the ovarian-adenocarcinoma cell line IGROV1.
Accumulating evidence suggests that integrins, which participate in many complex cellular processes, are important for tumor progression and metastasis. In order to understand the role of these cell-surface receptors and of their ligands in the biological behavior of ovarian tumor cells, we have examined the expression of integrins in the human ovarian-adenocarcinoma cell line IGROV1. These cells expressed the alpha v sub-unit and used it to attach on vitronectin (Vn). A monoclonal antibody (MAb) (69-6-5) specific to alpha v blocked the attachment of IGROV1 cells on Vn and fibrinogen (Fg), but not on fibronectin (FN) and other adhesive ligands. Immunoprecipitation of surface biotinylated cells followed by Western blotting showed that the alpha v sub-unit was associated with beta 3, but not with beta 1, beta 5 or beta 6. When cells were cultivated on glass coverslips or on Vn sub-stratum in serum-free medium, immunofluorescence staining with MAb 69-6-5 revealed the presence of alpha v at cell-cell contacts and at focal contacts, supporting its active participation in adhesion as part of a functional heterodimer. Furthermore, immunofluorescence data showed the presence of Vn as a fibrillar network in IGROV1 cells cultivated on FN-coated slides. These results suggest that alpha v beta 3 and its Vn ligand may play a important role in the behavior of ovarian epithelial tumors.
Carreiras
F
F
Laboratoire de Cancérologie Expérimentale, Centre F. Baclesse, Caen, France.
Lehmann
M
M
Sichel
F
F
Marvaldi
J
J
Gauduchon
P
P
Le Talaer
J Y
JY
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D002448:Cell Adhesion / Q000502:physiology; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D046911:Macromolecular Substances; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D019030:Receptors, Vitronectin / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D019096:Vitronectin / Q000378:metabolism
D000911:Antibodies, Monoclonal; D046911:Macromolecular Substances; D019030:Receptors, Vitronectin; D019096:Vitronectin; D005340:Fibrinogen