A novel Streptomyces restriction endonuclease, Sse1825I, cleaving at 5'-GG/GWCCC-3'.
We isolated and characterized from a Streptomyces species a new class-II restriction endonuclease, which recognizes the palindromic heptanucleotide sequence [sequence: see text] (where W = A or T) and cleaves double-stranded DNA after the second G in this sequence. This Sse1825I enzyme cleaves phage lambda DNA at one site, adenovirus type 2 DNA at eight sites, but does not cleave pBR322, SV40, ColE1, pUC18 and pUC19, and replicative forms of M13mp18 and M13mp19, and phiX174 DNAs.
Nomura
Y
Y
Biotechnology research Laboratories, Takara Shuzo Co., Ltd. Otsu, Shiga, Japan.
Ishino
Y
Y
Kimizuka
F
F
Kato
I
I
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity
D009838:Oligodeoxyribonucleotides; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific