title: Removal of adrenal steroids from the medium reverses the stimulating effect of catecholamines on corticotropin-releasing hormone neurons in organotypic cultures.
abstract: An organotypic culture system of anterior hypothalamic slices was developed for studying the secretory responses of corticotropin-releasing hormone (CRH) neurons to corticosteroid-catecholamine interactions. The standard culture medium included 5% horse serum containing 50 micrograms/l cortisol. In 1- to 3-day cultures, the tissue viability was demonstrated by the presence of arginine vasopressin immunolabeled perikarya and axons in the paraventricular nucleus and by sustained tissue concentrations of CRH (around 50 pg/mg protein). However, immunoreactive CRH neurons were not detectable in cultures in the standard medium. Exposure of cultures to high K+ (56 mM) in the medium induced a ten-fold increase in basal CRH release which was completely abolished in a Ca(2+)-free medium containing 2 mM EGTA. Noradrenaline (NA) triggered CRH release in a dose-dependent (1-20 microM) and time-dependent (0.5-6 h) manner. Removal of corticosteroids from the media by charcoal treatment led to (1) the visualization of immunolabelled CRH perikarya and fibers and a 55% rise in CRH content of the paraventricular nucleus tissue and (2) to a five-fold increase in CRH release. Both effects were reversed by supplementation of the culture medium with corticosterone (50 micrograms/l). Under steroid-free conditions, NA (1-10 microM) not only failed to induce CRH release, but strongly inhibited the consistent baseline in CRH release. This was reminiscent of a similar corticosteroid-dependent inversion of the NA effect on the hypothalamic-pituitary-adrenal axis described in vivo. Overall, these results are direct evidence of complex corticosteroid-catecholamine interrelationships as major regulatory factors of the hypothalamic-pituitary-adrenal axis.
authors.affiliation: Endocrinological Neurobiology Laboratory, URA 1197 CNRS, University of Montpellier 2, France.
authors.forename: A
authors.initials: A
authors.lastname: Szafarczyk
authors.forename: E
authors.initials: E
authors.lastname: Feuvrier
authors.forename: P
authors.initials: P
authors.lastname: Siaud
authors.forename: G
authors.initials: G
authors.lastname: Rondouin
authors.forename: M
authors.initials: M
authors.lastname: Lacoste
authors.forename: S
authors.initials: S
authors.lastname: Gaillet
authors.forename: F
authors.initials: F
authors.lastname: Malaval
authors.forename: I
authors.initials: I
authors.lastname: Assenmacher
mesh_terms: D000305:Adrenal Cortex Hormones / Q000008:administration & dosage* / Q000502:physiology; D000818:Animals; D001127:Arginine Vasopressin / Q000032:analysis; D002395:Catecholamines / Q000494:pharmacology*; D003346:Corticotropin-Releasing Hormone / Q000032:analysis / Q000378:metabolism*; D003470:Culture Media; D007032:Hypothalamus, Anterior / Q000187:drug effects / Q000502:physiology*; D007150:Immunohistochemistry; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D009924:Organ Culture Techniques; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000305:Adrenal Cortex Hormones; D002395:Catecholamines; D003470:Culture Media; D001127:Arginine Vasopressin; D003346:Corticotropin-Releasing Hormone; D011188:Potassium; D009638:Norepinephrine