Ligand binding analysis of interleukin-2 receptor complexes using surface plasmon resonance.
In this study we have employed surface plasmon resonance to examine the kinetic binding constants of a new class of soluble cytokine receptor complexes. The solution assembly of both homomeric and heteromeric interleukin-2 receptor ectodomain complexes has been achieved using coiled-coil molecular recognition. These complexes were immobilized on a biosensor surface and the kinetic binding constants were determined. The values obtained for these parameters compared favorably with those reported in studies of comparable cell surface complexes. The combination of these methods provides a powerful approach to the ligand interactions of cytokine receptors.
Wu
Z
Z
Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, NH 03755, USA.
Johnson
K W
KW
Ciardelli
T L
TL
D000936:Antigen-Antibody Complex / Q000276:immunology*; D015374:Biosensing Techniques; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D011485:Protein Binding / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000032:analysis* / Q000378:metabolism*; D013057:Spectrum Analysis / Q000379:methods*
D000936:Antigen-Antibody Complex; D008565:Membrane Proteins; D015375:Receptors, Interleukin-2