cDNA cloning and protein analysis of a bovine dermal allergen with homology to psoriasin.
Immunoscreening of a cDNA library from bovine skin led to isolation of clones coding for an allergen named BDA11. Sequence analysis of the clones revealed that they can encode a protein of 11.6 kDa with a predicted pI of 5.19. Allergenicity of BDA11 was verified by the IgE reactivity in cattle-allergic patients' sera with the recombinant protein produced in Escherichia coli. A biochemically purified native allergen of 11 kDa from bovine dander was identified as BDA11 by peptide sequencing. Homology comparisons showed that BDA11 had a 63.4% amino acid identity with human psoriasin. Psoriasin is a calcium-binding protein expressed in keratinocytes, and it is strongly up-regulated in psoriatic skin. BDA11 also had segments homologous with calcium-binding proteins from three other species.
Rautiainen
J
J
Department of Clinical Microbiology, University of Kuopio, Finland.
Rytkönen
M
M
Parkkinen
S
S
Pentikäinen
J
J
Linnala-Kankkunen
A
A
Virtanen
T
T
Pelkonen
J
J
Mäntyjärvi
R
R
D000485:Allergens / Q000737:chemistry* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D000939:Epitopes / Q000032:analysis; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D017433:Protein Structure, Secondary; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; D017386:Sequence Homology, Amino Acid; D012867:Skin / Q000276:immunology*
D000485:Allergens; D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D000939:Epitopes; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; C089242:S100A7 protein, human