title: The importance of the quality of water in Limulus Amebocyte Lysate tests.
abstract: Commercial pharmaceutical waters were evaluated regarding their interference in the chromogenic Limulus Amebocyte Lysate (LAL) test. Two of the four tested waters strongly inhibited the U.S. Reference Standard Endotoxin, while a Control Standard Endotoxin was less affected. The inhibitory effect could be mimiced by adding trace amounts of certain metal ions to a non-interfering water. In conclusion, the water used in the LAL test must be chosen with great care.
authors.affiliation: Chromogenix AB, Mölndal, Sweden.
authors.forename: K I
authors.initials: KI
authors.lastname: Dunér
mesh_terms: D004731:Endotoxins / Q000633:toxicity*; D004926:Escherichia coli; D005618:Fresh Water / Q000032:analysis*; D008033:Limulus Test / Q000379:methods*; D012015:Reference Standards; D012492:Salts / Q000032:analysis
chemical_list: D004731:Endotoxins; D012492:Salts