Identification of fibroblast growth factors in bovine cheese whey.
Acidic and basic fibroblast growth factors (FGF) were identified in bovine cheese whey after partial purification using a two step procedure. Cation-exchange chromatography produced a mitogen-rich extract which was loaded on to a heparin-sepharose column and eluted stepwise with 0.8, 1.2 and 2.0 M-NH4HCO3. Mitogenic activity was found in all three fractions by cell growth assays using Balb/c-3T3 fibroblasts. Immunoblotting identified acidic FGF in the 1.2 M-eluate and basic FGF in the 2.0 M-eluate, but neither acidic nor basic FGF was detected in the 0.8 M-fraction. Quantitative radioreceptor assays indicated 5.8 ng of acidic FGF-like activity and 19.8 ng of basic FGF-like activity per 1 whey in the appropriate eluates. This study represents the first direct demonstration of FGF in milk.
Rogers
M L
ML
Cooperative Research Centre for Tissue Growth and Repair, Adelaide, Australia.
Belford
D A
DA
Francis
G L
GL
Ballard
F J
FJ
D016475:3T3 Cells; D000818:Animals; D001681:Biological Assay; D002412:Cations; D002417:Cattle; D002455:Cell Division; D002611:Cheese / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D016220:Fibroblast Growth Factor 1 / Q000032:analysis*; D016222:Fibroblast Growth Factor 2 / Q000032:analysis*; D005347:Fibroblasts / Q000166:cytology; D006493:Heparin / Q000378:metabolism; D015151:Immunoblotting; D051379:Mice; D008807:Mice, Inbred BALB C; D011869:Radioligand Assay
D002412:Cations; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D006493:Heparin