Developmental and age-related alterations in rat brain presynaptic dopaminergic mechanisms.
Age-related changes in both pre- and post-synaptic components of dopamine neurons have been demonstrated in humans as well as in animals. Our study was designed to examine the effects of age on presynaptic DA neurons. To assess the developmental changes in rat striatal dopamine carrier, we used [3H]GBR 12935, which binds selectively to this transporter. In addition we monitored changes in amphetamine- and KCl-induced [3H]DA release from rat striatal slices. We were able to demonstrate age dependent changes in DA transporter density, which reached a peak at age 3 months. Amphetamine-induced released of stored DA was exactly reversed, with a nadir at age 3 months. We assumed that the combination of low DA transporter level with increased transporter-mediated DA release may have a major compensatory role with respect to the maintenance of dopaminergic transmission during normal development, aging and neuro-degenerative diseases.
Gordon
I
I
Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
Weizman
R
R
Rosenne
E
E
Rehavi
M
M
D000367:Age Factors; D000375:Aging / Q000378:metabolism*; D000661:Amphetamine / Q000494:pharmacology; D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism* / Q000502:physiology; D002352:Carrier Proteins / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism / Q000502:physiology; D004298:Dopamine / Q000378:metabolism* / Q000502:physiology; D050483:Dopamine Plasma Membrane Transport Proteins; D008297:Male; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D010879:Piperazines / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D017729:Presynaptic Terminals / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D009435:Synaptic Transmission
D002352:Carrier Proteins; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D010879:Piperazines; C044630:1-(2 (diphenylmethoxy)ethyl)-4-(3-phenylpropyl)piperazine; D000661:Amphetamine; D011188:Potassium; D004298:Dopamine