title: Variation in antigenicity and infectivity of derivatives of Borrelia burgdorferi, strain B31, maintained in the natural, zoonotic cycle compared with maintenance in culture.
abstract: The original isolate of Borrelia burgdorferi, strain B31, can be maintained in vitro indefinitely. A number of studies have demonstrated that there are recognizable changes in the genetic composition of the spirochete after more than 60 passages. We have maintained B31 in the natural zoonotic cycle of transmission of infection between laboratory mice and laboratory-reared Ixodes ticks. To determine whether similar changes occur in the natural transmission cycle, we reisolated strain B31 from mouse skin at the fifth zoonotic cycle. This reisolated derivative had the same infectivity as the parent B31 strain, had lost the 8-kb supercoiled plasmid present in B31, and induced a gross serum antibody response indistinguishable from the B31 immune response. Analysis of antigen expression with monoclonal antibodies generated against B31, however, showed differential expression of a subset of antigens between B31 and the isolated derivative.
authors.affiliation: Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA.
authors.forename: W T
authors.initials: WT
authors.lastname: Golde
authors.forename: M C
authors.initials: MC
authors.lastname: Dolan
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000911:Antibodies, Monoclonal / Q000276:immunology; D001426:Bacterial Proteins / Q000032:analysis; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity*; D003470:Culture Media; D018884:Ixodes / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR
chemical_list: D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; D003470:Culture Media