The glucose-dependent transactivation activity of ABF1 on the expression of the TDH3 gene in yeast.
Autonomously replicating sequence binding factor 1 (ABF1) has been implicated in the control of a variety of gene expressions in Saccharomyces cerevisiae. In this paper evidence is presented that ABF1 is involved in the glucose-dependent expression of the TDH3 gene which encodes glyceraldehyde-3-phosphate dehydrogenase. ABF1 binds to consensus sites located between -420 and -250, and between +77 and +200, and acts as a transactivator in an orientation-independent manner on both upstream and downstream sites. TDH3-lacZ fusions having an ABF1 consensus motif showed glucose-dependent expression of TDH3, whereas in the abf1 mutant strain JCA35 glucose-dependent expression disappeared. These findings suggest that ABF1 functions as a glucose-dependent transactivator for the expression of the TDH3 gene.
Jung
S Y
SY
Department of Molecular Biology, Seoul National University, Korea.
Yoo
H Y
HY
Kim
Y H
YH
Kim
J
J
Rho
H M
HM
D016384:Consensus Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D000431:Ethanol / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000494:pharmacology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics*; D005990:Glycerol / Q000494:pharmacology; D009154:Mutation; D010766:Phosphorylation; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D015533:Transcriptional Activation
C061886:ABF1 protein, S cerevisiae; D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000431:Ethanol; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005947:Glucose; D005990:Glycerol