Allogeneic killing by earthworm effector cells.
We observed spontaneous allogeneic cytotoxicity by coelomocytes (Lumbricus terrestris) using three assays: trypan blue, lactate dehydrogenase release and chromium-51 release. Cell-cell contact may not be essential to effect cytotoxicity, since killing of allogeneic cells occurred in pooled allogeneic coelomic fluid derived from worms raised in two different geographic locales. We observed no significant spontaneous cytotoxicity against autogeneic target coelomocytes haptenated with 2,4,6-trinitrobenzene sulfonic acid; however, coelomocytes effected significant spontaneous cytotoxicity against haptenated allogeneic targets. These results support the view that earthworm coelomocytes can act as effector cells that can specifically kill nonself target cells.
Suzuki
M M
MM
Department of Anatomy and Cell Biology, School of Medicine, University of California Los Angeles 90024-1763, USA.
Cooper
E L
EL
D000818:Animals; D000917:Antibody Formation / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000494:pharmacology; D002450:Cell Communication; D002860:Chromium Radioisotopes; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000187:drug effects / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology*; D007694:Killer Cells, Natural / Q000276:immunology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007962:Leukocytes / Q000276:immunology; D009835:Oligochaeta / Q000166:cytology*; D014343:Trypan Blue / Q000032:analysis
D015815:Cell Adhesion Molecules; D002860:Chromium Radioisotopes; D007770:L-Lactate Dehydrogenase; D014343:Trypan Blue