Assessment of minimal residual disease in acute promyelocytic leukaemia with t(15;17) by chromosome painting.
To detect the minimal residual disease (MRD) in acute promyelocytic leukaemia patients treated with all-trans retinoic acid, we compared the sensitivity of metaphase fluorescence in situ hybridization (FISH) with conventional analysis of G-banded metaphases. 5 out of 6 patients studied at diagnosis showed the t(15;17) translocation. 4 out of 5 patients carrying t(15;17) achieved complete remission and conventional cytogenetic conversion. In 3 cases the whole chromosome painting (WCP) probe 17 discovered one normal chromosome 17 and two fragments indicative of t(15;17) persistence. The FISH-WCP technique seems to be highly sensitive and recommendable in monitoring leukaemias with specific chromosome rearrangements.
Temperani
P
P
Department of Medical Sciences, University of Modena, Italy.
Vaccari
P
P
Giacobbi
F
F
Gandini
G
G
Ferrari
M G
MG
Emilia
G
G
D000328:Adult; D000368:Aged; D002871:Chromosome Banding; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D015473:Leukemia, Promyelocytic, Acute / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual; D014178:Translocation, Genetic; D014212:Tretinoin / Q000627:therapeutic use
D014212:Tretinoin