title: Disruption of the architectural factor HMGI-C: DNA-binding AT hook motifs fused in lipomas to distinct transcriptional regulatory domains.
abstract: Lipomas are one of the most common mesenchymal neoplasms in humans. They are characterized by consistent cytogenetic aberrations involving chromosome 12 in bands q14-15. Interestingly, this region is also the site of rearrangement for other mesenchymally derived tumors. This study demonstrates that HMGI-C, an architectural factor that functions in transcriptional regulation, has been disrupted by rearrangement at the 12q14-15 chromosomal breakpoint in lipomas. Chimeric transcripts were isolated from two lipomas in which HMGI-C DNA-binding domains (AT hook motifs) are fused to either a LIM or an acidic transactivation domain. These results, identifying a gene rearranged in a benign neoplastic process that does not proceed to a malignancy, suggest a role for HMGI-C in adipogenesis and mesenchyme differentiation.
authors.affiliation: Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854, USA.
authors.forename: H R
authors.initials: HR
authors.lastname: Ashar
authors.forename: M S
authors.initials: MS
authors.lastname: Fejzo
authors.forename: A
authors.initials: A
authors.lastname: Tkachenko
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: J A
authors.initials: JA
authors.lastname: Fletcher
authors.forename: S
authors.initials: S
authors.lastname: Weremowicz
authors.forename: C C
authors.initials: CC
authors.lastname: Morton
authors.forename: K
authors.initials: K
authors.lastname: Chada
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D015321:Gene Rearrangement / Q000235:genetics*; D025743:HMGA2 Protein; D006609:High Mobility Group Proteins / Q000235:genetics* / Q000502:physiology; D006801:Humans; D008067:Lipoma / Q000737:chemistry / Q000235:genetics*; D008648:Mesoderm; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis; D014158:Transcription, Genetic / Q000235:genetics; D015533:Transcriptional Activation
chemical_list: D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D025743:HMGA2 Protein; D006609:High Mobility Group Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm