Modified poly(glycidyl methacrylate-co-ethylene dimethacrylate) continuous rod columns for preparative-scale ion-exchange chromatography of proteins.
A continuous rod of porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) has been prepared by a free radical polymerization within the confines of a 300 x 8 mm I.D. chromatographic column. The epoxide groups of the rod have been modified by a reaction with diethylamine that affords ionizable functionalities required for the ion-exchange chromatographic mode. The properties of this rod column have been characterized and the column has been used successfully for the chromatographic separation of proteins. The column exhibits a dynamic capacity that exceeds 300 mg at a flow velocity of 200 cm/min. An excellent selectivity allows the separation of up to 300 mg of a protein mixture in a single run.
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Cornell University, Department of Chemistry, Baker Laboratory, Ithaca, NY 14853-1301, USA.
Fréchet
J M
JM
D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D003207:Conalbumin / Q000032:analysis; D004852:Epoxy Compounds; D008689:Methacrylates; D009113:Muramidase / Q000032:analysis; D010047:Ovalbumin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D014361:Trypsin Inhibitors / Q000032:analysis
D004852:Epoxy Compounds; D008689:Methacrylates; D011506:Proteins; D014361:Trypsin Inhibitors; D003207:Conalbumin; D012710:Serum Albumin, Bovine; C004919:ethylene dimethacrylate; D010047:Ovalbumin; D009113:Muramidase; C007870:glycidyl methacrylate