Cycle delay in irradiated cytochalasin-induced polykaryons. Does cytoskeleton status affect cell cycle checkpoints?
Following exposure of CHO-K1 cells to 137Cs irradiation at doses up to 20Gy, a delay in G2 was observed to occur in cells permitted to divide normally, while cells induced to become giants by means of cytochalasin B demonstrated a minimal delay in the transition 2C-8C suggesting that the inhibition of cytokinesis results in modification of one or more cell cycle checkpoints. We postulate that this may occur as a consequence of damage tolerance, or by a feedback loop resulting from the reorganisation of the cytoskeleton that precludes cytokinesis.
Davies
H E
HE
Radiation Science Laboratory, Velindre Hospital, Cardiff, Wales, United Kingdom.
Court
J B
JB
D000818:Animals; D016466:CHO Cells / Q000166:cytology / Q000528:radiation effects; D002453:Cell Cycle / Q000187:drug effects* / Q000528:radiation effects; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002588:Cesium Radioisotopes; D006224:Cricetinae; D003572:Cytochalasins / Q000494:pharmacology*; D003599:Cytoskeleton / Q000187:drug effects / Q000528:radiation effects; D015726:Giant Cells / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D013997:Time Factors
D002588:Cesium Radioisotopes; D003572:Cytochalasins