title: Characterization of a cDNA encoding the protein moiety of a putative arabinogalactan protein from Lycopersicon esculentum.
abstract: The nucleotide sequence of a cDNA prepared from poly(A)+ RNA from Lycopersicon esculentum fruit codes for a protein, M(r) 20,812, with features representative of the protein core of arabinogalactan proteins. The deduced amino acid sequence resembles that of peptides of arabinogalactan proteins isolated from carrot and rose and is most similar to the sequence of tryptic peptides from Lolium multiflorum (Gleeson et al., Biochem J 264 (1989) 857-862). The similar sequences include a number of Ala-Pro repeats, a feature considered distinctive of arabinogalactan proteins. The amino acid composition is similar to that of the peptide core of the Lolium multiflorum arabinogalactan protein; alanine, serine and proline account for 57% of the polypeptide. The mRNA corresponding to the cDNA sequence was detected in roots, leaves and fruit. The levels of mRNA are reduced in older leaves, in fruit that have commenced ripening and in leaves and fruit that have been wounded.
authors.affiliation: Division of Horticulture, CSIRO, North Ryde, NSW, Australia.
authors.forename: B J
authors.initials: BJ
authors.lastname: Pogson
authors.forename: C
authors.initials: C
authors.lastname: Davies
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D018076:DNA, Complementary / Q000235:genetics; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D009088:Mucoproteins / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D018076:DNA, Complementary; D009088:Mucoproteins; D010940:Plant Proteins; C006619:arabinogalactan proteins