title: Serum concentrations of MIP-1 alpha and interleukin-8 in patients suffering from acute Plasmodium falciparum malaria.
abstract: The chemokines are a superfamily of small proteins secreted primarily by leukocytes and related by a conserved four-cystein motif. In the present study we investigated the serum levels of macrophage inflammatory protein 1 alpha (MIP-1 alpha) and interleukin-8 (IL-8). MIP-1 alpha is a neutrophil chemotactic protein important in acute and chronic inflammation. Recent studies demonstrated that MIP-1 alpha may also act as potent inhibitor of hemopoetic stem cell proliferation, which may be important in the development of prolonged anemia in patients suffering from Plasmodium falciparum malaria. IL-8 serum concentrations correlate with severity and outcome of infectious diseases. Moreover, recent reports indicate that IL-8 plays a major role in fatal gram-negative sepsis. It was the aim of this study to investigate the time course of MIP-1 alpha and IL-8 concentrations in patients suffering from acute P. falciparum infection. Blood samples of 20 patients suffering from severe P. falciparum malaria were investigated. MIP-1 alpha and IL-8 concentrations were determined using ELISA technique at admission, on Days 7, 14, 21, and 28. Maximal concentrations of MIP-1 alpha and IL-8 were found on Day 14, at a time when parasites were not detected in the smears. The serum levels of IL-8 on the day of admission were correlated to the parasite count. No correlation was seen between the hematokrit values and the MIP-1 alpha concentrations at any time.
authors.affiliation: Department of Infectious Diseases, University of Vienna, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Burgmann
authors.forename: U
authors.initials: U
authors.lastname: Hollenstein
authors.forename: C
authors.initials: C
authors.lastname: Wenisch
authors.forename: F
authors.initials: F
authors.lastname: Thalhammer
authors.forename: S
authors.initials: S
authors.lastname: Looareesuwan
authors.forename: W
authors.initials: W
authors.lastname: Graninger
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000740:Anemia / Q000209:etiology; D037621:Artemisinins; D000077332:Artesunate; D001769:Blood / Q000469:parasitology; D001831:Body Temperature; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006400:Hematocrit; D006801:Humans; D016209:Interleukin-8 / Q000097:blood*; D019402:Macrophage Inflammatory Proteins; D016778:Malaria, Falciparum / Q000276:immunology* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D015846:Monokines / Q000097:blood*; D012717:Sesquiterpenes / Q000627:therapeutic use; D013785:Thailand
chemical_list: D037621:Artemisinins; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016207:Cytokines; D016209:Interleukin-8; D019402:Macrophage Inflammatory Proteins; D015846:Monokines; D012717:Sesquiterpenes; D000077332:Artesunate; C031327:artemisinin