Constitutively expressed Oct-2 prevents immunoglobulin gene silencing in myeloma x T cell hybrids.
Recent experiments involving disruption of the Oct-2 gene have shown that this largely B cell-restricted transcription factor is not required in the early stages of B cell development. However, B cells that lack Oct-2 may be blocked from differentiation past the surface immunoglobulin-positive stage. To identify a possible function for Oct-2 in the late stage immunoglobulin-secreting cell, we have used the method of somatic cell fusion. When the immunoglobulin-producing myeloma MPC11 is fused to a T lymphoma, Oct-2 production ceases, as does the expression of immunoglobulin, J chain, and several other B cell-specific gene products. In the present study, we show that by preventing the loss of Oct-2 in the hybrid cells, we can preserve expression of all other tested B cell-specific genes. These results establish a central role for Oct-2 in maintaining the genetic program of the immunoglobulin-secreting plasmacyte.
Radomska
H S
HS
Department of Biological Sciences, Hunter College, City University of New York, New York 10021, USA.
Shen
C P
CP
Kadesch
T
T
Eckhardt
L A
LA
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D001483:Base Sequence; D002460:Cell Line; D004247:DNA / Q000032:analysis; D004268:DNA-Binding Proteins / Q000502:physiology; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D006801:Humans; D006822:Hybrid Cells; D007144:Immunoglobulin J-Chains / Q000096:biosynthesis; D007142:Immunoglobulin gamma-Chains / Q000096:biosynthesis / Q000235:genetics; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis / Q000235:genetics; D016399:Lymphoma, T-Cell; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009101:Multiple Myeloma; D009687:Nuclear Proteins / Q000378:metabolism; D050813:Octamer Transcription Factor-2; D012333:RNA, Messenger / Q000032:analysis; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014162:Transfection
D004268:DNA-Binding Proteins; D007144:Immunoglobulin J-Chains; D007142:Immunoglobulin gamma-Chains; D007145:Immunoglobulin kappa-Chains; D009687:Nuclear Proteins; D050813:Octamer Transcription Factor-2; C058435:POU2F2 protein, human; C489206:Pou2f2 protein, mouse; D012333:RNA, Messenger; D014157:Transcription Factors; D004247:DNA