title: Characterization of defective RNAs derived from RNA 3 of the Fny strain of cucumber mosaic cucumovirus.
abstract: Two defective RNAs (designated D RNA 3 alpha and D RNA 3 beta) were found to be associated with the Fny strain of cucumber mosaic cucumovirus but not with the Sny strain after serial passages in a tobacco host. The D RNAs were derived from RNA 3 by single, in-frame deletions within the 3a open reading frame. A full-length cDNA clone from which biologically active transcripts can be produced in vitro has been constructed for D RNA 3 beta. This transcript can be replicated in tobacco plants infected with subgroup I and II cucumber mosaic cucumovirus strains and with peanut stunt cucumovirus. Translation of D RNA 3 beta in vitro produced a 20-kDa peptide, which was consistent with the predicted coding capacity of the deleted 3a open reading frame. D RNA 3 beta was also associated with polyribosomes isolated from infected tobacco plants. The presence of the D RNAs had no apparent effect upon helper virus yield or symptom production.
authors.affiliation: Plant Biology Division, Samuel Roberts Noble Foundation, Inc., Ardmore, Oklahoma 73402-2180, USA.
authors.forename: M V
authors.initials: MV
authors.lastname: Graves
authors.forename: M J
authors.initials: MJ
authors.lastname: Roossinck
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017799:Cucumovirus / Q000235:genetics*; D017931:DNA Primers; D003673:Defective Viruses / Q000235:genetics*; D006378:Helper Viruses / Q000502:physiology; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D012367:RNA, Viral