title: Assessment of cellular proliferation of sebaceous neoplasms by AgNOR counts and immunohistochemical demonstrations of PCNA and Ki-67.
abstract: We assessed cellular proliferation of sebaceous neoplasms by AgNOR counts and the immunohistochemical demonstration of proliferating cell nuclear antigen (PCNA) and Ki-67, using formalin-fixed and paraffin-embedded tissue specimens. We used three categories of sebaceous neoplasms: four cases of sebaceoma, three cases of basal cell carcinoma with sebaceous differentiation (BCSD), and seven cases of sebaceous carcinoma (SC). Significant differences were noted between SC and non-SC tumors (sebaceoma and BCSD) in AgNOR counts and semi-quantitative grading of PCNA and Ki-67 labelling indices (P < 0.01). When a cut-off value of 6 was chosen, the AgNOR value discriminated SC from non-SC tumors with high specificity and sensitivity. When a cut-off value of 25% was chosen, PCNA and Ki-67 labelling indices also discriminated between these tumors. Significant differences were not observed between sebaceoma and BCSD with PCNA and Ki-67 labelling indices. AgNOR counts of BCSD were a little higher than those of sebaceoma, but the number of cases was too small to perform statistical assessment. We consider AgNOR counts and semi-quantitative grading of PCNA and Ki-67 labelling indices to be useful in differentiating SC from BCSD and sebaceoma.
authors.affiliation: Department of Dermatology, Yamagata University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ansai
authors.forename: S
authors.initials: S
authors.lastname: Koseki
authors.forename: Y
authors.initials: Y
authors.lastname: Hozumi
authors.forename: S
authors.initials: S
authors.lastname: Kondo
authors.forename: K
authors.initials: K
authors.lastname: Aso
mesh_terms: D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis; D009697:Nucleolus Organizer Region; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012626:Sebaceous Gland Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D012627:Sebaceous Glands / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure
chemical_list: D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D018809:Proliferating Cell Nuclear Antigen