title: Growth-rate-dependent transcription initiation from the dam P2 promoter.
abstract: Transcription of the dam gene in Escherichia coli is dependent on growth rate. Using single-copy promoter::lacZYA fusions we found that of the five promoter regions which affect dam expression, only the P2 promoter shows growth-rate dependence. The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point.
authors.affiliation: Department of Pharmacology, University of Massachusetts Medical School, Worcester 01655, USA.
authors.forename: L J
authors.initials: LJ
authors.lastname: Rasmussen
authors.forename: A
authors.initials: A
authors.lastname: Løbner-Olesen
authors.forename: M G
authors.initials: MG
authors.lastname: Marinus
mesh_terms: D001483:Base Sequence; D003106:Colon / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008780:Methyltransferases / Q000096:biosynthesis* / Q000235:genetics; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D013379:Substrate Specificity; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D008780:Methyltransferases; C058693:Dam methyltransferase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); C499294:dam protein, E coli; D001616:beta-Galactosidase