Immunoelectron microscopic localization of one of the spore germination proteins, GerAB, in Bacillus subtilis spores.
Ultrastructural localization of GerAB, one of the proteins of Bacillus subtilis spores related to L-alanine-initiated germination, was investigated by immunoelectron microscopy with antipeptide (residues 61 to 80 of GerAB) antiserum and a colloidal gold-immunoglobulin G complex. Immunogold particles were visualized in the boundary region between the cortex and coat of dormant spores, and they were broadly dispersed into the cortex region after germination.
Sakae
Y
Y
Department of Microbiology, Nagoya City University Medical School, Japan.
Yasuda
Y
Y
Tochikubo
K
K
D000907:Antibodies, Bacterial; D001412:Bacillus subtilis / Q000254:growth & development / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002451:Cell Compartmentation; D008565:Membrane Proteins / Q000302:isolation & purification*; D016253:Microscopy, Immunoelectron; D013171:Spores, Bacterial / Q000254:growth & development / Q000648:ultrastructure
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; C096462:GerAB protein, Bacillus subtilis; D008565:Membrane Proteins