title: Expression and function of Pax 1 during development of the pectoral girdle.
abstract: Pax 1 is a member of the paired-box containing gene family. Expression has previously been observed in the developing sclerotomes and later in the anlagen of the intervertebral discs. Analysis of Pax 1-deficient undulated mice revealed an important role for this gene in the development of the axial skeleton, in which Pax 1 apparently functions as a mediator of notochordal signals during sclerotome differentiation. Here we demonstrate that Pax 1 is also transiently expressed in the developing limb buds. A comparative phenotypic analysis of different undulated alleles shows that this expression is of functional significance. In mice that are mutant for the Pax 1 gene severe developmental abnormalities are found in the pectoral girdle. These include fusions of skeletal elements which would normally remain separate, and failures in the differentiation of blastemas into cartilaginous structures. Although Pax 1 is also expressed in the developing hindlimb buds and Wolffian ridge, no malformations could be detected in the corresponding regions of Pax 1 mutant mice. These findings show that, in addition to its role in the developing vertebral column, Pax 1 has an important function in the development of parts of the appendicular skeleton.
authors.affiliation: Laboratory of Eukaryotic Molecular Genetics, MRC National Institute for Medical Research, Mill Hill, London, UK.
authors.forename: P M
authors.initials: PM
authors.lastname: Timmons
authors.forename: J
authors.initials: J
authors.lastname: Wallin
authors.forename: P W
authors.initials: PW
authors.lastname: Rigby
authors.forename: R
authors.initials: R
authors.lastname: Balling
mesh_terms: D000818:Animals; D001483:Base Sequence; D001842:Bone and Bones / Q000196:embryology*; D002968:Clavicle / Q000196:embryology; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D005121:Extremities / Q000196:embryology*; D015870:Gene Expression; D005801:Genes, Homeobox; D017403:In Situ Hybridization; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D051761:Paired Box Transcription Factors; D010641:Phenotype; D012540:Scapula / Q000196:embryology; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology
chemical_list: D004268:DNA-Binding Proteins; D051761:Paired Box Transcription Factors; D014157:Transcription Factors; C070835:PAX1 transcription factor