title: Genomic structure and sequence of the Fugu rubripes glucose-6-phosphate dehydrogenase gene (G6PD).
abstract: To investigate the organization of the compact genome of the puffer fish Fugu rubripes at the level of an individual housekeeping gene, we have cloned and sequenced the glucose-6-phosphate dehydrogenase (G6PD) gene from this fish and compared it to the corresponding human gene. The intron/exon structure of the two genes is identical throughout the protein coding regions. The puffer fish gene is four times smaller than the human gene--the difference is accounted for by the fact that the puffer fish gene has smaller introns. Intron 2 is the largest intron in both species. We have constructed a molecular phylogeny for 10 G6PD protein sequences. The sequences fall in the expected arrangement based on established phylogenetic relationships, with the Plasmodium falciparum sequence the most widely diverged.
authors.affiliation: Department of Haematology, Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.
authors.forename: P J
authors.initials: PJ
authors.lastname: Mason
authors.forename: D J
authors.initials: DJ
authors.lastname: Stevens
authors.forename: L
authors.initials: L
authors.lastname: Luzzatto
authors.forename: S
authors.initials: S
authors.lastname: Brenner
authors.forename: S
authors.initials: S
authors.lastname: Aparicio
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA; D005400:Fishes, Poisonous / Q000235:genetics*; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
chemical_list: D004247:DNA; D005954:Glucosephosphate Dehydrogenase