Isolation and localization of transcribed sequences on human chromosome 22.
Recently, we reported the generation of a heteronuclear (hn) cDNA library from a human x rodent somatic cell hybrid retaining human chromosome 22. Here, we report the characterization and localization of 12 cDNA derived clones from this library. Human-specific cDNA sequences have been selectively amplified by inter-Alu PCR. To exclude Alu transcripts, only clones with inserts larger than 500 bp were analyzed. Ten of the 12 clones were localized by PCR on chromosome 22, with four clones mapping on additional chromosomes. One PCR-localized clone and two additional clones were mapped on chromosome 22 by in situ hybridization. Transcripts in human cells were identified for seven of the eight clones analyzed by RT-PCR. None of the clones showed significant sequence similarities within the GenBank and EMBL databases, indicating that these clones represent previously unknown genes. This is the first report on the isolation of chromosome 22-specific transcripts from a human x rodent somatic cell hybrid.
Gimbel
W
W
Department of Human Genetics, Medical School, University of Saarland, Homburg/Saar, Germany.
Klein
V
V
Brass
N
N
Fischer
U
U
Piontek
K
K
Overmyer
K
K
Göttert
E
E
Zang
K D
KD
Meese
E
E
D000818:Animals; D001483:Base Sequence; D002892:Chromosomes, Human, Pair 22; D006224:Cricetinae; D017931:DNA Primers; D018076:DNA, Complementary; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid; D014158:Transcription, Genetic
D017931:DNA Primers; D018076:DNA, Complementary