title: Identifying genes within microdissected genomic DNA: isolation of brain expressed genes from a translocation region associated with inherited mental illness.
abstract: An improved protocol has been developed for physical enrichment of cDNA sequences by hybridization to genomic DNA. When applied to microdissection recombinants derived from a translocation breakpoint region associated with inherited mental illness, a single cycle of the procedure permitted enriched cDNAs to be visualized directly by agarose gel electrophoresis. Hybridization screening of a library of clones derived from the enriched cDNAs, employing the genomic resource as a probe, led to the identification of six novel gene fragments. This general approach to the isolation of regionally encoded genes could be applied to any subchromosomal interval as a first step towards global transcription map construction.
authors.affiliation: Molecular Genetics Section, MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.
authors.forename: A J
authors.initials: AJ
authors.lastname: Brookes
authors.forename: E M
authors.initials: EM
authors.lastname: Slorach
authors.forename: K L
authors.initials: KL
authors.lastname: Evans
authors.forename: M L
authors.initials: ML
authors.lastname: Thomson
authors.forename: C M
authors.initials: CM
authors.lastname: Gosden
authors.forename: W J
authors.initials: WJ
authors.lastname: Muir
authors.forename: D J
authors.initials: DJ
authors.lastname: Porteous
mesh_terms: D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D001921:Brain / Q000378:metabolism*; D002144:Callithrix; D002415:Cats; D002417:Cattle; D002878:Chromosomes, Human, Pair 1; D006224:Cricetinae; D018076:DNA, Complementary / Q000235:genetics; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D015723:Gene Library; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011618:Psychotic Disorders / Q000235:genetics*; D011817:Rabbits; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D014178:Translocation, Genetic
chemical_list: D018076:DNA, Complementary