Hepatocyte growth factor/scatter factor expressed in follicular papilla cells stimulates human hair growth in vitro.
Hepatocyte growth factor/scatter factor (HGF/SF) is a multifunctional polypeptide which acts as mitogen, motogen, or morphogen. In this study, we examined the effect of HGF/SF on human hair growth using organ and cell culture systems. HGF/SF was found to stimulate hair length and DNA synthesis in hair follicles at increasing concentrations up to 10 ng/ml (P < 0.05 and P < 0.01, respectively). HGF/SF stimulated [3H]thymidine incorporation by hair bulb-derived keratinocytes with the strongest response at 30 ng/ml of HGF/SF (P < 0.05). Cultured follicular papilla cells secreted HGF/SF, measured by an enzyme-linked immunoassay, in response to interleukin 1-alpha (IL1-alpha, 10 ng/ml), tumor necrosis factor-alpha (TNF-alpha, 10 ng/ml), or tetradecanoylphorbolacetate (100 nM) at levels ranging from 0.2 to 0.3 ng/mg protein/48 h. HGF/SF mRNA expressions, measured by the reverse transcription-polymerase chain reaction, were detected in follicular papilla cells, and were also stimulated by the three reagents. Transforming growth factor-beta (10 ng/ml) suppressed both protein and mRNA levels. These results suggest that hair follicle elongation induced by HGF/SF in organ culture occurs partly due to the mitogenic activity of HGF/SF expressed in follicular papilla cells on hair bulb-derived keratinocytes.
Shimaoka
S
S
Department of Dermatology, Juntendo University, School of Medicine, Tokyo, Japan.
Tsuboi
R
R
Jindo
T
T
Imai
R
R
Takamori
K
K
Rubin
J S
JS
Ogawa
H
H
D001483:Base Sequence; D002478:Cells, Cultured; D046508:Culture Techniques; D006197:Hair / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism*; D017228:Hepatocyte Growth Factor / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D015603:Keratinocytes / Q000187:drug effects; D015335:Molecular Probes / Q000235:genetics; D008969:Molecular Sequence Data; D009924:Organ Culture Techniques; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D014158:Transcription, Genetic
D015335:Molecular Probes; D012333:RNA, Messenger; D017228:Hepatocyte Growth Factor