title: The primary structure of a hemorrhagic factor, HR2b, from the venom of Okinawa habu (Trimeresurus flavoviridis).
abstract: The complete amino acid sequence of a hemorrhagic factor, HR2b, from the venom of Okinawa habu was determined. The hemorrhagic factor was fragmented by CNBr cleavage, trypsin, staphylococcal protease V8 and lysyl endopeptidase digestions. The resulting peptides were purified on high performance chromatography, and sequenced by Edman degradation. HR2b was composed of 204 amino acids with pyroglutamyl residue at the amino terminus, and the calculated mol. wt based on the amino acid composition was 23,335. There are three disulfide linkages in the primary structure. The consensus sequence (His-Glu-Xaa-Xaa-His) for zinc-binding site of zinc-requiring metalloproteinases was found in the structure. The primary structure of HR2b shows a significant similarity with that of HR2a of Amami habu venom; 98.5% identity.
authors.affiliation: Tropical Technology Center Ltd., Okinawa, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Iha
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Qi
authors.forename: T
authors.initials: T
authors.lastname: Kannki
authors.forename: Y
authors.initials: Y
authors.lastname: Tomihara
authors.forename: K
authors.initials: K
authors.lastname: Yonaha
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D003435:Crotalid Venoms / Q000032:analysis* / Q000737:chemistry* / Q000201:enzymology; D003488:Cyanogen Bromide / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism
chemical_list: D000596:Amino Acids; D003435:Crotalid Venoms; C052578:hemorrhagic toxin HR-2b; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D014357:Trypsin; C036813:lysyl endopeptidase; D003488:Cyanogen Bromide