title: Small extrachromosomal nucleic acid segments in protozoan parasites.
abstract: Viruses have been described in the following protozoa: Babesia spp., Trichomonas vaginalis, Giardia lamblia, Leishmania braziliensis and Eimeria spp. In order to study the Babesia bovis virus, merozoites have been prepared from the blood of infected cattle. Agarose gel electrophoresis of nucleic extracts from the bovine protozoa B. bovis and Babesia bigemina were separated into genomic DNA and at least two additional nucleic acids. One molecule with a relative mobility of 5.5 kilobase pairs (kbp) was identified as a double-stranded RNA virus-like particle. Another 6.2 kbp DNA molecule had sequences related to mitochondrial genome.
authors.affiliation: Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
authors.forename: I
authors.initials: I
authors.lastname: Hotzel
authors.forename: R
authors.initials: R
authors.lastname: Kabakoff
authors.forename: L S
authors.initials: LS
authors.lastname: Ozaki
mesh_terms: D000818:Animals; D001403:Babesia / Q000821:virology; D016793:Babesia bovis / Q000302:isolation & purification / Q000821:virology*; D001404:Babesiosis / Q000097:blood* / Q000821:virology; D002417:Cattle; D002418:Cattle Diseases; D016054:DNA, Protozoan / Q000097:blood; D004279:DNA, Viral / Q000097:blood; D004539:Eimeria / Q000821:virology; D056890:Eukaryota / Q000821:virology*; D016829:Giardia lamblia / Q000821:virology; D007892:Leishmania braziliensis / Q000821:virology; D014246:Trichomonas vaginalis / Q000821:virology; D014779:Virus Replication
chemical_list: D016054:DNA, Protozoan; D004279:DNA, Viral