title: Reduced nitric oxide responsive soluble guanylyl cyclase activity in the superior temporal cortex of patients with Alzheimer's disease.
abstract: Particulate and soluble guanylyl cyclase activities were studied in postmortem temporal cortex from a series of Alzheimer's disease patients and matched control subjects. Particulate guanylyl cyclase activity was not significantly different between groups. In contrast, the Vmax values for basal and sodium nitroprusside-stimulated soluble guanylyl cyclase activities were approximately 50% lower in the Alzheimer's disease cases, compared to controls. This difference between groups was statistically significant for sodium nitroprusside-stimulated, but not for the basal, enzyme activities. These results provide the first evidence for a loss of nitric oxide responsive guanylyl cyclase activity in Alzheimer's disease brain.
authors.affiliation: Karolinska Institute, Department of Clinical Neuroscience and Family Medicine, Huddinge, Sweden.
authors.forename: W L
authors.initials: WL
authors.lastname: Bonkale
authors.forename: B
authors.initials: B
authors.lastname: Winblad
authors.forename: R
authors.initials: R
authors.lastname: Ravid
authors.forename: R F
authors.initials: RF
authors.lastname: Cowburn
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000378:metabolism*; D001344:Autopsy; D006152:Cyclic GMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006162:Guanylate Cyclase / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D013702:Temporal Lobe / Q000378:metabolism*
chemical_list: D009569:Nitric Oxide; D006162:Guanylate Cyclase; D006152:Cyclic GMP; D008274:Magnesium