title: PCR analysis of the short tandem repeat (STR) system HUMVWA31. Allele and genotype frequencies in an Italian population sample.
abstract: A population study in a sample of 211 unrelated individuals from 2 cities in North and Central Italy was carried out to investigate the short tandem repeat (STR) system HUMVWA31. Separation of PCR- amplified DNA fragments was performed by high-resolution horizontal denaturing polyacrylamide gel electrophoresis (PAGE) followed by silver staining. The 7 common alleles were found, together with a new smaller allele. Distribution of the observed genotypes did not deviate from Hardy-Weinberg (H-W) equilibrium. The power of discrimination for this locus was 0.93 and the chance of exclusion was 0.61. Good agreement was found between the allele frequencies in 2 Italian population samples and previous studies on Caucasians. The results of this study suggest that this STR system may be a useful tool in forensic investigations.
authors.affiliation: Istituto di Medicina Legale, Università di Parma, Ospedale Maggiore, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Buscemi
authors.forename: N
authors.initials: N
authors.lastname: Cucurachi
authors.forename: R
authors.initials: R
authors.lastname: Mencarelli
authors.forename: A
authors.initials: A
authors.lastname: Tagliabracci
authors.forename: P
authors.initials: P
authors.lastname: Wiegand
authors.forename: S D
authors.initials: SD
authors.lastname: Ferrara
mesh_terms: D000483:Alleles; D000941:Antigens / Q000235:genetics*; D001801:Blood Stains; D004247:DNA / Q000235:genetics*; D005787:Gene Frequency / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D007558:Italy; D009838:Oligodeoxyribonucleotides / Q000235:genetics*; D010334:Paternity; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D015183:Restriction Mapping; D014841:von Willebrand Factor / Q000276:immunology
chemical_list: D000941:Antigens; D005819:Genetic Markers; D009838:Oligodeoxyribonucleotides; C029417:Von Willebrand antigen; D014841:von Willebrand Factor; D004247:DNA