title: Chronic encephalitis associated with epilepsy: immunohistochemical and ultrastructural studies.
abstract: Chronic encephalitis has been recognized as a cause of epilepsy since the work of Rasmussen et al. in the late 1950s. Despite this, few immunohistochemical studies of the affected brain tissue have been attempted. We have studied specimens of brain tissue from seven patients with this condition who underwent therapeutic multilobar cortical resection or hemispherectomy. Immunohistochemical studies were carried out using antibodies to glial fibrillary acidic protein (GFAP), proliferating cell nuclear antigen (PCNA, PC10), T lymphocytes (UCHL-1), B lymphocytes (L26), macrophages and microglia (HAM-56), and major histocompatibility complex molecules (LN3 and beta 2-microglobulin). Additionally, the results of preliminary immunohistochemical and ultrastructural investigation of possible immune complex deposition in blood vessel walls of affected brain tissue are presented. The pattern of GFAP immunoreactivity suggested a patchy and/or laminar disease process in most patients. GFAP immunoreactive cells were especially prominent around microvessels in some cases, suggesting an abnormality and perivascular collections of inflammatory cells, seen to a variable extent in all cases, contained abundant cells immunolabelled with UCHL-1, LN3 and beta 2-microglobulin. L26-labelled B lymphocytes were extremely sparse. Anti-PCNA frequently labelled microvascular endothelial cells, rare pericytes and occasional cells with microglial/macrophage morphology. The data suggest that chronic encephalitis found in patients with epilepsy results from patchy but widespread parenchymal brain injury, in the course of which cells of both microglial and lymphocyte series accumulate or proliferate within brain.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Pathology (Neuropathology), Beaumont Hospital, Dublin, Ireland.
authors.forename: M A
authors.initials: MA
authors.lastname: Farrell
authors.forename: O
authors.initials: O
authors.lastname: Droogan
authors.forename: D L
authors.initials: DL
authors.lastname: Secor
authors.forename: V
authors.initials: V
authors.lastname: Poukens
authors.forename: B
authors.initials: B
authors.lastname: Quinn
authors.forename: H V
authors.initials: HV
authors.lastname: Vinters
mesh_terms: D000293:Adolescent; D017668:Age of Onset; D000906:Antibodies; D001253:Astrocytes / Q000473:pathology; D002540:Cerebral Cortex / Q000648:ultrastructure; D002648:Child; D002675:Child, Preschool; D004660:Encephalitis / Q000473:pathology*; D004827:Epilepsy / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000473:pathology; D017628:Microglia / Q000473:pathology
chemical_list: D000906:Antibodies