HIV-1 DNA in fibroblast cultures infected with urine from HIV-seropositive cytomegalovirus (CMV) excretors.
Interactions between HIV-1 and CMV may be important in the pathogenesis of AIDS. We have studied whether active CMV infection alters the cell tropism of HIV-1 in dually-infected individuals. Urines from HIV-seropositive individuals excreting CMV were compared to urines from CMV non-excretors. Sixty-six urines from HIV-seropositive individuals were tested. Infectious HIV-1 was not detected in any of the concentrated urines tested. The urines were filtered, concentrated, DNase-treated and cultured on HIV-1 non-permissive human forestin fibroblasts. HIV-1 DNA was detected by PCR with pol gene primers in 5 of 39 MRHF cell cultures inoculated with CMV culture positive urine (p = 0.037). HIV-1 DNA was not detected by PCR in uninfected fibroblasts, in fibroblasts inoculated with CMV uninfected urine from 27 HIV-seropositive patients or in fibroblasts cultured with 9 CMV culture positive urines from 16 HIV-seronegative renal transplant recipients. Supernatant fluid from an HIV-1 PCR-positive culture was passaged onto another fibroblast monolayer, and these cells were negative for HIV-1 DNA. Direct inoculation of fibroblasts with HIV-1 did not yield evidence of infection by PCR. CMV infection may facilitate HIV-1 DNA entry into ordinarily non-permissive cells.
Margalith
M
M
Infectious Disease Unit, Massachusetts General Hospital, Harvard Medical School, Boston, USA.
D'Aquila
R T
RT
Manion
D J
DJ
Basgoz
N
N
Bechtel
L J
LJ
Smith
B R
BR
Kaplan
J C
JC
Hirsch
M S
MS
D002478:Cells, Cultured; D003586:Cytomegalovirus Infections / Q000652:urine*; D004279:DNA, Viral / Q000032:analysis*; D005347:Fibroblasts / Q000821:virology; D006679:HIV Seropositivity / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans
D004279:DNA, Viral