Double-sandwich enzyme-linked immunosorbent assay (ELISA) with murine monoclonal antibodies for detection of staphylococcal enterotoxin B.
Three monoclonal antibodies (MAbs) which react specifically with staphylococcal enterotoxin B (SEB) were studied for their suitability for use in ELISA. One of them (MAb B14) worked well as a coating antibody; MAb B12 was shown to be a good probing antibody (conjugated with peroxidase) when ELISA plates were coated with MAb B14. This effective pair of MAbs (B14-B12PO) is able to detect 0.625 ng SEB ml, and to distinguish between SEB and other proteins present in food extracts.
Goyache
J
J
Departamento de Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
Orden
J A
JA
Blanco
J L
JL
Doménech
A
A
Suárez
G
G
Gómez-Lucía
E
E
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004768:Enterotoxins / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005516:Food Microbiology; D008461:Meat Products / Q000382:microbiology; D051379:Mice; D008892:Milk / Q000382:microbiology; D013211:Staphylococcus aureus; D015014:Yogurt / Q000382:microbiology
D000911:Antibodies, Monoclonal; D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal