title: Serodiagnosis of Helicobacter pylori infection by enzyme-linked immunoelectrotransfer blot. Gastrointestinal Physiology Working Group of Cayetano Heredia University.
abstract: Immunoreactive bands in sera from 81 Peruvian adults (48 females and 33 males) aged between 18 and 85 years were detected using enzyme-linked immunoelectrotransfer blot with a glycine-extracted Helicobacter pylori antigen. Sensitivity and specificity of bands were determined by comparison with results of H. pylori obtained by culture and silver stain of gastric biopsies. A crude antigen and a commercial enzyme-linked immunosorbent assay (Pylori Stat, Whittaker) were also evaluated for their diagnostic utility. H. pylori was detected in 57/81 (70%) of the subjects. The crude antigen enzyme-linked immunosorbent assay and the commercial enzyme-linked immunosorbent assay kits were, respectively, 84% and 88% sensitive. Specificity of both was 92%. The glycine-extracted antigen produced ten bands. The best single diagnostic band was at 20 kDa (83% sensitive and 96% specific). Three bands (13, 23, 30 kDa) were 100% specific. The combination of two diagnostic bands 20 and 30 kDa was 90% sensitive and 96% specific. Low molecular weight bands could be useful for the diagnosis of H. pylori infection.
authors.affiliation: Department of Microbiology and Tropical Medicine Institute, Johns Hopkins University, Baltimore, MD 21205, USA.
authors.forename: G
authors.initials: G
authors.lastname: Madico
authors.forename: M
authors.initials: M
authors.lastname: Verástegui
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity