The TBP-TFIIA interaction in the response to acidic activators in vivo.
A yeast TBP mutant (N2-1) is described here that is defective specifically in responding to acidic activators in vivo. N2-1 does not support activation by Gal4, Ace1, and Gcn4, but appears unaffected for constitutive transcription, repression by the Cyc8-Tup1 and Not complexes, and transcription by polymerase I (Pol) and Pol III. In vitro, N2-1 fails to interact with TFIIA, but it associates normally with a TATA element, an acidic activation domain, and TFIIB. Fusion of the small subunit of TFIIA to N2-1 restores activation function in vivo. Thus, an efficient interaction between TBP and TFIIA is required for transcriptional activation in vivo.
Stargell
L A
LA
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Struhl
K
K
D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005656:Fungal Proteins / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D017874:Immediate-Early Proteins / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D011494:Protein Kinases / Q000502:physiology; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D016385:TATA Box; D035181:TATA-Box Binding Protein; D015534:Trans-Activators / Q000502:physiology*; D035561:Transcription Factor TFIIA; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015533:Transcriptional Activation
C060256:CUP2 protein, S cerevisiae; C065455:CYC8 protein, S cerevisiae; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C052130:GAL4 protein, S cerevisiae; D017874:Immediate-Early Proteins; D009687:Nuclear Proteins; D057126:Nuclear Receptor Subfamily 4, Group A, Member 2; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D035181:TATA-Box Binding Protein; C066502:TUP1 protein, S cerevisiae; D015534:Trans-Activators; D035561:Transcription Factor TFIIA; D014157:Transcription Factors; D011494:Protein Kinases; D012321:DNA-Directed RNA Polymerases