Identification of enterohaemorrhagic Escherichia coli by means of their production of enterohaemolysin.
With increasing interest in verocytotoxigenic Escherichia coli (VTEC) being associated with both human and animal infection, a simple system of rapidly identifying the majority of VTEC has been devised. This depends on the fact that most VTEC produce enterohaemolysin, which is rarely produced by non-VTEC. By employing two media, traditional sheep blood agar (SBA) and washed sheep blood agar supplemented with calcium (WSBA-Ca), enterohaemolysin-producing strains can be easily differentiated from other E. coli enabling most VTEC types, including ones belonging to serogroups other than O157, to be isolated.
Bettelheim
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Biomedical Reference Laboratory, VIDRL, Fairfield Hospital, Victoria, Australia.
D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis; D001427:Bacterial Toxins / Q000096:biosynthesis*; D002118:Calcium / Q000494:pharmacology*; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D006801:Humans; D012756:Sheep / Q000097:blood; D022622:Shiga Toxin 1; D014118:Toxins, Biological
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003470:Culture Media; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli; D022622:Shiga Toxin 1; D014118:Toxins, Biological; C066219:enterohemolysin; D002118:Calcium