Pancreatic tau related maps: biochemical and immunofluorescence analysis in a tumoral cell line.
In the present study, we report the existence of four tau-related microtubule-associated proteins (MAPs) of 48, 50, 55 and 58 kDa in a pancreatic exocrine cell line (AR4-2J). Using immunofluorescence, we demonstrate that these tau-related MAPs are associated with microtubules in AR4-2J cells. That colocalization is particularly striking on microtubules bundles in cellular extensions and is the first evidence for tau-related MAPs colocalization with microtubules in non-neuronal cells. As it has been often discussed for neuronal tau, the localization of tau-related proteins in AR4-2J cells suggests that these proteins may be involved in microtubule bundling.
Michalik
L
L
INSERM U.381, Ontogénèse et pathologie du systéme digestif, Strasbourg, France.
Neuville
P
P
Vanier
M T
MT
Launay
J F
JF
D000818:Animals; D000906:Antibodies / Q000032:analysis; D002460:Cell Line; D002846:Chromatography, Affinity; D005455:Fluorescent Antibody Technique; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000032:analysis* / Q000737:chemistry; D008870:Microtubules / Q000737:chemistry / Q000187:drug effects; D017239:Paclitaxel / Q000494:pharmacology; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000473:pathology*; D011817:Rabbits; D051381:Rats; D014404:Tubulin / Q000187:drug effects; D014747:Vinblastine / Q000494:pharmacology; D016875:tau Proteins / Q000032:analysis / Q000737:chemistry / Q000276:immunology
D000906:Antibodies; D008869:Microtubule-Associated Proteins; D014404:Tubulin; D016875:tau Proteins; D014747:Vinblastine; D017239:Paclitaxel