T cells, but not B cells, are required for bowel inflammation in interleukin 2-deficient mice.
Interleukin-2 (IL-2)-deficient (IL-2-/-) mice develop anemia and colonic inflammatory bowel disease. To elucidate the mechanism of this disease, we have bred IL-2-/- mice to two strains of immunodeficient mice, RAG-2-deficient (RAG-2-/-, lacking B and T cells) and JH-deficient mice (JH-/-, lacking B cells). IL-2-/-, RAG-2-/- double-mutant mice are disease free, while IL-2-/-, JH-/- double-mutant mice succumb to bowel disease at the same rate as IL-2-/- littermates. IL-2-/-, JH-/- mice do not, however, succumb to anemia. Thus, spontaneous intestinal inflammation in IL-2-/- mice requires mature T cells, not B cells, while anemia is dependent on B cells.
Ma
A
A
Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA.
Datta
M
M
Margosian
E
E
Chen
J
J
Horak
I
I
D000744:Anemia, Hemolytic, Autoimmune / Q000209:etiology / Q000276:immunology; D000818:Animals; D001323:Autoantibodies / Q000032:analysis; D001327:Autoimmune Diseases / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D001402:B-Lymphocytes / Q000276:immunology*; D003106:Colon / Q000473:pathology; D004268:DNA-Binding Proteins; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007144:Immunoglobulin J-Chains / Q000235:genetics; D007153:Immunologic Deficiency Syndromes / Q000150:complications / Q000235:genetics; D015212:Inflammatory Bowel Diseases / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D007376:Interleukin-2 / Q000172:deficiency* / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D018448:Models, Immunological; D009894:Opportunistic Infections / Q000150:complications; D011506:Proteins / Q000235:genetics; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000276:immunology*
D001323:Autoantibodies; D004268:DNA-Binding Proteins; D007143:Immunoglobulin Heavy Chains; D007144:Immunoglobulin J-Chains; D007376:Interleukin-2; D011506:Proteins; C499308:Rag2 protein, mouse; C064655:V(D)J recombination activating protein 2