Phase-contrast radiographs of nonstained rat cerebellar specimen.
Phase-contrast radiography using an x-ray interferometer is presented for observing organic matter. High sensitivity of phase-contrast radiography is demonstrated with a rat cerebellar specimen without staining it with a contrast medium. The layer structure of the cerebellum can be observed in the obtained image while there is no clear structure in the corresponding absorption-contrast image. Quantitative image analysis is made possible by converting an x-ray interference pattern to an x-ray phase shift distribution. The lipid distribution in the cerebellum is discussed by evaluating x-ray phase shifts before and after lipid removal.
Momose
A
A
Advanced Research Laboratory, Hitachi, Ltd., Saitama, Japan.
Fukuda
J
J
D000818:Animals; D002531:Cerebellum / Q000166:cytology* / Q000000981:diagnostic imaging*; D066298:In Vitro Techniques; D007368:Interferometry; D008433:Mathematics; D011859:Radiography / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D012680:Sensitivity and Specificity