Immunohistochemical changes in sigmoid colon after allogeneic and autologous bone marrow transplantation.
OBJECTIVE
To determine whether there are characteristic immunohistological changes in the colonic mucosa in acute graft versus host disease (GvHD).


METHODS
Consecutive allogeneic (n = 11) and autologous (n = 11) bone marrow transplant recipients underwent endoscopic biopsy of sigmoid mucosa before transplant and on day 30 post-transplant. Immunohistochemical staining and quantitation of intraepithelial and lamina propria mononuclear cells were undertaken using a panel of monoclonal antibodies and a Streptavidin-biotin alkaline phosphatase staining technique.


RESULTS
In the allogeneic group (nine of whom had clinical acute GvHD) there was a fivefold increase in lamina propria CD16+ mononuclear cells (3.1 +/- 4.3 to 17.0 +/- 12.2 per 100 lamina propria nucleated cells), compared with autologous transplant recipients in whom this rise was twofold (5.5 +/- 4.6 to 10.6 +/- 7.1 per 100 lamina propria nucleated cells). The CD16+ mononuclear cells had morphological appearances of tissue macrophages, but in neither the allogeneic nor autologous groups was there an increase in total macrophage numbers (CD14+). In patients with acute GvHD the lamina propria CD4+:CD8+ lymphocyte ratio fell (1.97 +/- 1.12 to 1.07 +/- 1.01), primarily because of a fall in the number of lamina propria CD4+ lymphocytes. In both allogeneic and autologous groups there was a fall in intraepithelial lymphocyte numbers, but there was no change in CD19+ (B cell), CD25+ (interleukin-2 receptor positive) or CD56+ (natural killer) cell numbers.


CONCLUSIONS
Following bone marrow transplantation, there appears to be upregulation of lamina propria tissue macrophage CD16 (an Fc receptor for IgG), a change which is more noticeable after allogeneic transplantation and which may be related to the development of acute GvHD. In patients with acute GvHD there was a fall in the lamina propria CD4+:CD8+ lymphocyte ratio. If these changes are functionally important, they may have significant implications for understanding the pathogenesis of GvHD.
Forbes
G M
GM
Department of Gastroenterology, Royal Perth Hospital, Western Australia.
Erber
W N
WN
Herrmann
R P
RP
Davies
J M
JM
Collins
B J
BJ
D000208:Acute Disease; D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D016026:Bone Marrow Transplantation / Q000276:immunology* / Q000473:pathology; D016516:CD4-CD8 Ratio; D012809:Colon, Sigmoid / Q000276:immunology* / Q000473:pathology; D005260:Female; D006086:Graft vs Host Disease / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000276:immunology; D007958:Leukocyte Count; D016131:Lymphocyte Subsets / Q000473:pathology; D008297:Male; D008875:Middle Aged; D017452:Receptors, IgG / Q000032:analysis; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous
D015703:Antigens, CD; D017452:Receptors, IgG