Bovine elastin and kappa-elastin secondary structure determination by optical spectroscopies.
Elastin is the macromolecular polymer of tropoelastin molecules responsible for the elastic properties of tissues. The understanding of its specific elasticity is uncertain because its structure is still unknown. Here, we report the first experimental quantitative determination of bovine elastin secondary structures as well as those of its corresponding soluble kappa-elastin. Using circular dichroism and Fourier transform infrared and near infrared Fourier transform Raman spectroscopic data, we estimated the secondary structure contents of elastin to be approximately 10% alpha-helices, approximately 45% beta-sheets, and approximately 45% undefined conformations. These values were very close to those we had previously determined for the free monomeric tropoelastin molecule, suggesting thus that elastin would be constituted of a closely packed assembly of globular beta structural class tropoelastin molecules cross-linked to form the elastic network (liquid drop model of elastin architecture). The presence of a strong hydration shell is demonstrated for elastin, and its possible contribution to elasticity is discussed.
Debelle
L
L
Laboratoire de Spectroscopies et Structures Biomoéculaires, Université de Reims Champagne-Ardenne, INSERM Unité 314, CHRU, France.
Alix
A J
AJ
Jacob
M P
MP
Huvenne
J P
JP
Berjot
M
M
Sombret
B
B
Legrand
P
P
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004548:Elasticity; D004549:Elastin / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods; D014333:Tropoelastin / Q000737:chemistry
D000596:Amino Acids; D014333:Tropoelastin; D004549:Elastin