Conversion of D-arabinose to D-erythroascorbic acid and oxalic acid in Sclerotinia sclerotiorum.
D-glycero-Pent-2-enono-1,4-lactone (trivial name: D-erythroascorbic acid) occurs in the phytopathogen, Sclerotinia sclerotiorum (Lib.) de Bary, where it has a potential role as precursor of oxalic acid. On Glc/yeast/malt medium, S. sclerotiorum produces only nominal amounts of D-erythroascorbic acid but even partial replacement of Glc by D-Ara increases production of erythroascorbic acid and oxalic acid. Use of D-[1-14C]-, -[3-14C]-, or -[6-14C]Glc and D-[5-3H]-, -[2-14C,5-3H]-, or -[UL-14C]Ara provide additional information on erythroascorbic acid biosynthesis and cleavage. The latter process resembles that obtained by peroxygenation of erythroascorbic acid in alkaline solution. An unknown erythroascorbic acid-like compound also occurs in both Glc- and Ara-based cultures.
Loewus
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Institute of Biological Chemistry, Washington State University, Pullman 99164-6340, USA.
Saito
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Suto
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Maring
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D001089:Arabinose / Q000378:metabolism*; D001203:Ascomycota / Q000254:growth & development / Q000378:metabolism*; D001205:Ascorbic Acid / Q000302:isolation & purification / Q000378:metabolism*; D001711:Biotransformation; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D005947:Glucose / Q000378:metabolism; D010070:Oxalates / Q000302:isolation & purification / Q000378:metabolism*; D019815:Oxalic Acid; D011865:Radioisotope Dilution Technique; D014316:Tritium
D002250:Carbon Radioisotopes; D010070:Oxalates; D014316:Tritium; C005056:isoascorbic acid; D019815:Oxalic Acid; D001089:Arabinose; D005947:Glucose; D001205:Ascorbic Acid