Expression of a yeast gene can be blocked by insertion of short yeast DNA fragments between a UAS and the TATA box.
We have constructed a plasmid, pOV10, which facilitates the introduction of putative upstream activating sequences (UAS) or upstream repressing sequences (URS) from yeast genes into plasmids containing CYCl-lacZ fusions. We have observed that the insertion of yeast sequences from 155 to 195 bp between the UAS and the TATA box of a CYCl-lacZ fusion gene can block beta-galactosidase expression. It is suggested that this block is related to the formation of nucleosomes on the DNA.
Vincent
O
O
Instituto de Investigaciones Biomédicas del C.S.I.C., Madrid, Spain.
Gancedo
J M
JM
D003001:Cloning, Molecular / Q000379:methods; D003574:Cytochrome c Group / Q000235:genetics; D045304:Cytochromes c; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors; D010957:Plasmids; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D016385:TATA Box; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
C014059:CYC1 protein, S cerevisiae; D003574:Cytochrome c Group; D029701:Saccharomyces cerevisiae Proteins; D045304:Cytochromes c; D001616:beta-Galactosidase