Characterization of immunoreactive adrenomedullin in human plasma and urine.
Adrenomedullin(AM) is a novel vasodilator peptide recently isolated from pheochromocytoma. Using a specific and sensitive radioimmunoassay for human AM, we have characterized immunoreactive AM in human plasma and urine. Patients with chronic renal failure had about five-fold higher plasma immunoreactive AM levels than normal subjects, which did not change before and after hemodialysis. Immunoreactive AM was present in normal human urine, whose concentrations were about six-fold greater than those in human plasma. Reverse-phase HPLC of human plasma and urine revealed that immunoreactive AM emerged as a single peak at a position identical to that of authentic human AM(1-52). These data suggest that circulating AM is cleared by the kidney and urinary excretion of AM may be derived from glomerular filtration and/or its renal production.
Sato
K
K
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Hirata
Y
Y
Imai
T
T
Iwashina
M
M
Marumo
F
F
D053607:Adrenomedullin; D000328:Adult; D000368:Aged; D000959:Antihypertensive Agents / Q000097:blood* / Q000276:immunology / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood / Q000652:urine; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000097:blood* / Q000276:immunology / Q000652:urine*; D011863:Radioimmunoassay / Q000379:methods; D012015:Reference Standards; D012016:Reference Values; D006435:Renal Dialysis; D012680:Sensitivity and Specificity
D000959:Antihypertensive Agents; D010455:Peptides; D053607:Adrenomedullin