Bowman-Birk proteinase inhibitor (BBI) modulates radiosensitivity and radiation-induced differentiation of human fibroblasts in culture.
The radiosensitivity and differentiation pattern of cultured normal human fibroblasts was analysed as a function of treatment of the cells with the Bowman-Birk proteinase inhibitor (BBI). Upon irradiation with doses from 0 to 8 Gy normal human fibroblasts are induced to a premature terminal differentiation within 14-21 days of postirradiation incubation. Treatment of the cells with 10 microM BBI for 2 h prior to the irradiation procedure resulted in a significant shift of the radiation survival curve, increased SF2 values 0.63 vs. 0.84 and the cell type composition of the test fibroblast cultures. Upon pretreatment with BBI the radiation-induced premature terminal differentiation of progenitor fibroblasts to postmitotic fibrocytes could significantly be inhibited. Based on this data, it can be postulated that BBI may serve as a radioprotector of normal fibroblasts which are involved in radiation-induced tissue injuries like radiation fibrosis.
Dittmann
K
K
Department of Radiotherapy, Eberhard-Karls-University, Tübingen, Germany.
Löffler
H
H
Bamberg
M
M
Rodemann
H P
HP
D002454:Cell Differentiation / Q000187:drug effects / Q000528:radiation effects; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects*; D006801:Humans; D008938:Mitosis / Q000187:drug effects / Q000528:radiation effects; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000187:drug effects*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D012867:Skin / Q000166:cytology; D013234:Stem Cells / Q000187:drug effects / Q000528:radiation effects; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000494:pharmacology*
D011837:Radiation-Protective Agents; D014358:Trypsin Inhibitor, Bowman-Birk Soybean