title: Effects of cisplatin and selenite on the level of thiols in pig blood platelets.
abstract: The level of free sulfhydryl groups of glutathione and proteins in pig blood platelets after short lasting incubation (30 min.) of these cells with cisplatin (cis-diamminedichloroplatinum II) and sodium selenite was investigated in vitro. The concentration of the platelet thiols after sodium selenite or cisplatin treatment distinctly decreased (p < 0.001). Pretreatment of blood platelets with sodium selenite at the low concentration (0.1 microM) protected platelet glutathione against the reaction with cisplatin (p < 0.001). The present study shows that in blood platelets after the incubation with cisplatin the GS-platinum complex was formed. The results of our experiments demonstrated also that the extracellular GSH (1 mM) had the protective effect against cisplatin-induced peroxidation of platelet lipids.
authors.affiliation: Department of Biochemistry, University of Lódź, Poland.
authors.forename: B
authors.initials: B
authors.lastname: Wachowicz
authors.forename: T
authors.initials: T
authors.lastname: Krajewski
authors.forename: B
authors.initials: B
authors.lastname: Olas
authors.forename: H M
authors.initials: HM
authors.lastname: Zbikowska
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002945:Cisplatin / Q000494:pharmacology*; D008315:Malondialdehyde / Q000037:antagonists & inhibitors / Q000097:blood; D018038:Sodium Selenite / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000097:blood*; D013552:Swine / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D018038:Sodium Selenite; D002945:Cisplatin