title: The effects of blockade of retinal cell action potentials on ocular growth, emmetropization and form deprivation myopia in young chicks.
abstract: To investigate the influence of brain mediated functions on control of ocular growth, young chicks were treated monocularly with intravitreally injected tetrodotoxin (TTX) to block retinal ganglion cell action potentials. TTX injections (0.7 micrograms in 7 microliters) were given on day 6 after hatching in both binocularly open and monocularly deprived chicks. Injections were repeated every 48 hr for a period of 8 days (TTX-open; TTX-MD). Control groups of animals received intravitreally injected phosphate buffered saline (PBS-open; PBS-MD) to one eye on the same schedule. There was a minimum of eight animals in each group. Recovery from form-deprivation myopia during blockade of retinal cell action potentials was also investigated. Results demonstrate that blockade of retinal cell action potentials by TTX produces reduced growth of the anterior segment of the eye and crystalline lens in both binocularly open and MD chicks. Blockade of retinal cell action potentials does not prevent form-deprivation induced vitreous chamber elongation and myopia. Form deprived myopic eyes were found to emmetropize despite blockade of retinal ganglion cell action potentials giving further evidence for local ocular control of emmetropization. Blockade of retinal ganglion cell action potentials did not prevent changes in choroidal thickness in eyes developing axial myopia or eyes recovering from induced myopia.
authors.affiliation: Department of Optometry and Vision Sciences, University of Wales, College of Cardiff.
authors.forename: N A
authors.initials: NA
authors.lastname: McBrien
authors.forename: H O
authors.initials: HO
authors.lastname: Moghaddam
authors.forename: C L
authors.initials: CL
authors.lastname: Cottriall
authors.forename: E M
authors.initials: EM
authors.lastname: Leech
authors.forename: L M
authors.initials: LM
authors.lastname: Cornell
mesh_terms: D000200:Action Potentials / Q000187:drug effects*; D000818:Animals; D000869:Anterior Eye Segment / Q000254:growth & development; D001699:Biometry; D002645:Chickens; D002829:Choroid / Q000473:pathology; D003315:Cornea / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D005123:Eye / Q000254:growth & development*; D007908:Lens, Crystalline / Q000254:growth & development / Q000473:pathology; D009216:Myopia / Q000209:etiology* / Q000473:pathology; D012165:Retinal Ganglion Cells / Q000502:physiology*; D012683:Sensory Deprivation / Q000502:physiology*; D013779:Tetrodotoxin / Q000494:pharmacology; D013997:Time Factors
chemical_list: D013779:Tetrodotoxin