title: Acidification of rabbit corneal endothelium during contact lens wear in vitro.
abstract: Contact lens wear causes significant epithelial and stromal acidosis. In this study, we tested whether lens wear can cause endothelial acidosis as well. Rabbit corneas were isolated and perfused in vitro. The endothelial intracellular pH (pHi) was measured with a pH sensitive fluorescent probe (BCECF). Three conditions were examined: 1) Polymethylmethacrylate (PMMA) and rigid gas-permeable (RGP) contact lens wear using a range of oxygen transmissibility (Dk/L) from 0 to 121, 2) epithelial hypoxia produced by exposure to oligomycin/sodium azide solution or epithelial perfusion with 100% N2 equilibrated Ringer's solution, and 3) epithelial exposure to Ringer's equilibrated with 5% CO2, balance air. PMMA and RGP contact lens wear acidified endothelial cells by 0.23 +/- 0.01 (n = 23) and 0.11 +/- 0.01 pH units (n = 23), respectively, within twenty min of lens insertion. Epithelial hypoxia, induced by sodium azide and oligomycin, reversibly acidified the endothelium by 0.04 +/- 0.01 pH units (n = 4). However, epithelial hypoxia induced by perfusion with 100% N2 equilibrated Ringer's did not have a significant effect on endothelial pHi. Introduction of 5% CO2 to the epithelium, acidified the endothelium by 0.15 +/- 0.02 pH units (n = 7) within 10 min. We conclude that contact lens wear can significantly acidify corneal endothelial cells. The endothelial pHi change is caused almost exclusively by a build up of CO2 behind the lens; hypoxia having very little contribution. As expected, RGP contact lenses induced less endothelial acidosis than PMMA controls.
authors.affiliation: Morton D. Sarver Center for Cornea and Contact Lens Research, University of California, School of Optometry, Berkeley 94720, USA.
authors.forename: C
authors.initials: C
authors.lastname: Giasson
authors.forename: J A
authors.initials: JA
authors.lastname: Bonanno
mesh_terms: D000138:Acidosis / Q000378:metabolism*; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D015687:Cell Hypoxia / Q000502:physiology; D003261:Contact Lenses; D003315:Cornea / Q000166:cytology / Q000378:metabolism; D004728:Endothelium, Corneal / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D020366:Methylmethacrylate; D008768:Methylmethacrylates; D010100:Oxygen / Q000378:metabolism; D011817:Rabbits
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D008768:Methylmethacrylates; D002245:Carbon Dioxide; D020366:Methylmethacrylate; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein; D010100:Oxygen