Possible involvement of mononuclear cells stimulated with specific allergen from asthmatic patients in ICAM-1 expression on the eosinophilic cell line.
Adhesion molecules, particularly intercellular adhesion molecule-1 (ICAM-1), recently have been considered to play a key role in inflammatory processes in asthma. Thus, from the point of view of cell interactions between mononuclear cells and eosinophils, we examined whether the supernatant of mononuclear cells (MNC) obtained from mite-allergic asthmatic patients cultured with specific allergen is involved in ICAM-1 expression using an eosinophilic cell line (EoL). ICAM-1 expression was induced by the supernatant of MNC from mite-allergic asthmatic patients stimulated with mite allergen as well as by a combination of IL-3, GM-CSF, and IL-5. Thus, we could conclude that some cytokines produced by specific allergen-stimulated MNC in asthmatics might be involved in allergic inflammation through the induction of adhesion molecule expression such as ICAM-1 on eosinophils in asthma or allergic disorders.
Chihara
J
J
Fourth Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.
Kurachi
D
D
Yamamoto
T
T
Yamada
H
H
Hayashi
N
N
Nakajima
S
S
D000485:Allergens / Q000494:pharmacology*; D000818:Animals; D001249:Asthma / Q000276:immunology*; D002460:Cell Line; D004804:Eosinophils / Q000276:immunology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008925:Mites / Q000276:immunology
D000485:Allergens; D018799:Intercellular Adhesion Molecule-1