Evidence for giant linear plasmids in the ascomycete Podospora anserina.
In the extrachromosomal mutant AL2 of the ascomycete Podospora anserina longevity is correlated with the presence of the linear mitochondrial plasmid pAL2-1. In addition to this autonomous genetic element, two types of closely related pAL2-1-homologous molecules were detected in the high-molecular-weight mitochondrial DNA (mtDNA). One of these molecules is of linear and the other of circular structure. Both molecules contain pAL2-1 sequences which appear to be integrated at the same site in the mtDNA. Sequence analysis of a DNA fragment cloned from one of these molecules revealed that it contains an almost full-length copy of pAL2-1. At the site of plasmid integration a 15-nucleotide AT-spacer and long inverted mtDNA sequences were identified. Finally, two giant linear plasmid-like DNAs of about 50 kbp and 70 kbp were detected in pulsed-field gels of mutant AL2. These molecules are composed of mtDNA and pAL2-1-specific sequences and may result from the integration of mtDNA sequences into linear plasmid pAL2-1.
Hermanns
J
J
Department of Molecular Biology of the Aging Processes, German Cancer Research Center, Heidelberg.
Asseburg
A
A
Osiewacz
H D
HD
D001483:Base Sequence; D015139:Blotting, Southern; D003573:Cytochrome b Group / Q000235:genetics; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D016521:Electrophoresis, Gel, Pulsed-Field; D005092:Exonucleases / Q000378:metabolism; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D013098:Xylariales / Q000235:genetics* / Q000254:growth & development
D003573:Cytochrome b Group; D004271:DNA, Fungal; D004272:DNA, Mitochondrial; D005092:Exonucleases