title: Immunoelectron microscopic localization of one of the spore germination proteins, GerAB, in Bacillus subtilis spores.
abstract: Ultrastructural localization of GerAB, one of the proteins of Bacillus subtilis spores related to L-alanine-initiated germination, was investigated by immunoelectron microscopy with antipeptide (residues 61 to 80 of GerAB) antiserum and a colloidal gold-immunoglobulin G complex. Immunogold particles were visualized in the boundary region between the cortex and coat of dormant spores, and they were broadly dispersed into the cortex region after germination.
authors.affiliation: Department of Microbiology, Nagoya City University Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Sakae
authors.forename: Y
authors.initials: Y
authors.lastname: Yasuda
authors.forename: K
authors.initials: K
authors.lastname: Tochikubo
mesh_terms: D000907:Antibodies, Bacterial; D001412:Bacillus subtilis / Q000254:growth & development / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002451:Cell Compartmentation; D008565:Membrane Proteins / Q000302:isolation & purification*; D016253:Microscopy, Immunoelectron; D013171:Spores, Bacterial / Q000254:growth & development / Q000648:ultrastructure
chemical_list: D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; C096462:GerAB protein, Bacillus subtilis; D008565:Membrane Proteins