title: Carbohydrate specificity of the Escherichia coli P-pilus papG protein is mediated by its N-terminal part.
abstract: The adherence of pyelonephritic Escherichia coli isolates to mammalian host cells is mediated by the P-pili structures on the bacterial surface. The protein constituting the distal part of the pili structure, papG, interacts with glycan receptors on the host cell. Variation in specificity for different glycoconjugates between the isolates, that may reflect variation in host tropism, has been correlated to three different classes of papG. Truncated variants of the class I, II and III papG adhesins were produced as fusion protein in E. coli and analysed for carbohydrate binding. The results showed that both carbohydrate binding and specificity of the papG adhesin resided in a linear part of the N-terminus of the protein.
authors.affiliation: Symbicom AB, Umeå, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Hansson
authors.forename: P
authors.initials: P
authors.lastname: Wallbrandt
authors.forename: J O
authors.initials: JO
authors.lastname: Andersson
authors.forename: M
authors.initials: M
authors.lastname: Byström
authors.forename: A
authors.initials: A
authors.lastname: Bäckman
authors.forename: A
authors.initials: A
authors.lastname: Carlstein
authors.forename: K
authors.initials: K
authors.lastname: Enquist
authors.forename: H
authors.initials: H
authors.lastname: Lönn
authors.forename: C
authors.initials: C
authors.lastname: Otter
authors.forename: M
authors.initials: M
authors.lastname: Strömqvist
mesh_terms: D018830:Adhesins, Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D001483:Base Sequence; D001665:Binding Sites; D050260:Carbohydrate Metabolism; D002846:Chromatography, Affinity; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000737:chemistry*; D006384:Hemagglutination; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D018830:Adhesins, Escherichia coli; C092426:PapG protein, E coli; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D036781:Fimbriae Proteins