Flow cytometric evaluation of erythrocyte response to oxidant stress.
Erythrocyte response to oxidant challenge has been used as an indicator of cell membrane tolerance to oxidant stress. This study describes a simple technique for evaluation of erythrocyte response to hydrogen peroxide oxidant stress using flow cytometry as an assay method. Challenged cells showed a detectable increase in fluorescence over the basal level. This increase in fluorescence was both concentration and time dependent. The test was standardized using 0.1 mM hydrogen peroxide and 2 h incubation. The standardized test showed a higher fluorescence of challenged umbilical cord erythrocytes compared to challenged adult red blood cells. We recommend further use of this test for examining other populations of red blood cells.
el-Rahman
A
A
Department of Biochemistry, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Hammouda
M A
MA
Fakeir
A
A
D000328:Adult; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects*; D005260:Female; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007231:Infant, Newborn; D015227:Lipid Peroxidation; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*
D006861:Hydrogen Peroxide