title: Observations on the use of purified follicle-stimulating hormone in the treatment of luteal phase defects.
abstract: We treated 18 infertile patients affected by histologically confirmed luteal phase deficiency with 75 IU of purified follicle-stimulating hormone (FSH) daily during the first 5 days of the cycle. Patients who were not pregnant after the first cycle of treatment underwent a second cycle. In the second cycle the daily doses of purified FSH were doubled if luteal phase deficiency had persisted during the first cycle. During the two cycles before treatment and during treatment, patients underwent an endometrial biopsy 1-3 days before the expected onset of menses. An assessment of progesterone serum concentrations was also performed on days 8, 6 and 4 before the expected onset of menses. Treatment was administered in a total of 33 cycles resulting in 30 ovulatory cycles. Six pregnancies were achieved. Among non-conception ovulatory cycles, 13 presented delayed endometrial dating and 11 normal endometrium. The mean +/- SD of the sum of the three progesterone determinations was 14.7 +/- 1.4 ng/ml in pretreatment cycles, 14.6 +/- 1.6 ng/ml in cycles with normalization of endometrial dating, 14.8 +/- 1.7 ng/ml in cycles with persistence of luteal phase deficiency and 30.4 +/- 3.0 ng/ml in conception cycles (P < 0.05 versus other groups). We conclude that purified FSH, if effective in the treatment of luteal phase deficiency, does not act through an increase in progesterone concentrations.
authors.affiliation: Department of Obstetrics and Gynecology, University of Naples, Federico II, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Di Carlo
authors.forename: P
authors.initials: P
authors.lastname: Affinito
authors.forename: M J
authors.initials: MJ
authors.lastname: Farace
authors.forename: A R
authors.initials: AR
authors.lastname: Gargiulo
authors.forename: F
authors.initials: F
authors.lastname: Zullo
authors.forename: C
authors.initials: C
authors.lastname: Nappi
mesh_terms: D000328:Adult; D001706:Biopsy; D004717:Endometrium / Q000473:pathology; D005069:Evaluation Studies as Topic; D005260:Female; D005640:Follicle Stimulating Hormone / Q000302:isolation & purification / Q000627:therapeutic use*; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000188:drug therapy*; D008183:Luteal Phase / Q000187:drug effects*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011446:Prospective Studies; D016896:Treatment Outcome
chemical_list: D011374:Progesterone; D005640:Follicle Stimulating Hormone