Acetylcholine esterase and peripherin mRNA level decrease in wobbler mouse.
Homozygote wobbler mice develop motoneurone degeneration. Throughout development the expression of choline acetyltransferase, of trkC receptor and F3 adhesion molecule genes is similar in wobbler and wild-type spinal cord. Acetylcholinesterase mRNA level instead is decreased to about 50% with respect to wild-type values in one forth of P5 and P10 wobbler progeny, putative wr/wr individuals; at P21 its expression is equally highly reduced in known homozygotes and it is reduced to 35% of normal values in about one half of the progeny, putative heterozygotes. Thus, similarly to medium neurofilament gene over-expression, reduced acetylcholinesterase gene expression is an early molecular marker for the wobbler mutation before onset of the illness.
Pernas-Alonso
R
R
International Institute of Genetics and Biophysics, CNR, Naples, Italy.
Schaffner
A E
AE
Hansen
C T
CT
Barker
J L
JL
di Porzio
U
U
D000110:Acetylcholinesterase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007381:Intermediate Filament Proteins / Q000235:genetics*; D008562:Membrane Glycoproteins; D051379:Mice; D008818:Mice, Neurologic Mutants; D008969:Molecular Sequence Data; D009046:Motor Neurons / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D009479:Neuropeptides / Q000235:genetics*; D064531:Peripherins; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics; D020812:Receptor, trkC; D017475:Receptors, Nerve Growth Factor / Q000235:genetics; D012016:Reference Values; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology / Q000254:growth & development
D015816:Cell Adhesion Molecules, Neuronal; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D064531:Peripherins; D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D020812:Receptor, trkC; D000110:Acetylcholinesterase