A subpopulation of large ganglion neurons express IsK protein mRNA: an in situ hybridization analysis in the rat eye.
Rat IsK protein is a 130-amino acid membrane protein and subserves generation of K+ outward current. The expression of this putative K+ ion channel mRNA was explored in the eye with in situ hybridization. The ganglion cell layer of the retina expressed IsK mRNA in a subpopulation of ganglion cells composed of large cell bodies. The hybridization-positive cells were scattered throughout the retina. The hybridization signal was also localized to the epithelial cells throughout the cornea. The results provided evidence for IsK message in neuronal cells. Expression of IsK message in the eye was thus shown to be restricted to particular cell types.
Tsukahara
Y
Y
Department of Anatomy, Kansai Medical University, Osaka, Japan.
Houtani
T
T
Ueyama
T
T
Ikeda
M
M
Nakanishi
S
S
Yamamoto
A
A
Tashiro
Y
Y
Sugimoto
T
T
D000818:Animals; D048429:Cell Size; D003315:Cornea / Q000737:chemistry; D004848:Epithelium / Q000737:chemistry; D005136:Eye Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D015221:Potassium Channels / Q000235:genetics*; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000737:chemistry*
D005136:Eye Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; C059983:potassium channel protein I(sk)