title: Microdissection of the Y chromosome and fluorescence in situ hybridization analysis of the sex chromosomes of lake trout, Salvelinus namaycush.
abstract: Lake trout, Salvelinus namaycush, is one of the few salmonids with morphologically differentiated sex chromosomes. Genetic analysis suggested that the sex-determining region of this species lies on the short arm of the Y chromosome. The differential arm of the Y chromosome was microdissected and the resulting DNA amplified in a sequence-independent manner. Amplified DNA was biotin labeled as a probe for fluorescence in situ hybridization (FISH). Strong hybridization signals were seen covering defined regions of both the Y and X chromosomes. Homeologous chromosomes of the ancestrally tetraploid genome were not identified by FISH with the Y probe, indicating diploidization of this region of the genome.
authors.affiliation: Department of Biological Sciences, University of Wisconsin-Milwaukee 53201, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Reed
authors.forename: S K
authors.initials: SK
authors.lastname: Bohlander
authors.forename: R B
authors.initials: RB
authors.lastname: Phillips
mesh_terms: D000818:Animals; D001483:Base Sequence; D002875:Chromosomes; D015342:DNA Probes; D005260:Female; D005821:Genetic Techniques; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D014337:Trout / Q000235:genetics*; D014960:X Chromosome; D014998:Y Chromosome
chemical_list: D015342:DNA Probes