Fluorescent substances and high molecular weight protein aggregates formed in rat heart mitochondria upon doxorubicin-induced lipid peroxidation.
A rat heart mitochondrial suspension was incubated with doxorubicin, FeCl3 and NADH. Fluorescent substances and high molecular weight protein aggregates were observed in the mitochondrial membranes upon the formation of thiobarbituric acid-reactive substances. Since both fluorescent substances and high molecular weight protein aggregates are retained in mitochondrial membranes, they can be of use in the clarification of the site of doxorubicin-induced lipid peroxidation.
Fukuda
F
F
Department of Biopharmaceutics, Tokyo College of Pharmacy, Japan.
Kitada
M
M
Horie
T
T
Awazu
S
S
D000818:Animals; D001665:Binding Sites; D002712:Chlorides; D004317:Doxorubicin / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D005290:Ferric Compounds / Q000494:pharmacology; D005453:Fluorescence; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D008970:Molecular Weight; D009243:NAD / Q000494:pharmacology; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D013050:Spectrometry, Fluorescence; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D002712:Chlorides; D005290:Ferric Compounds; D011506:Proteins; D017392:Thiobarbituric Acid Reactive Substances; D009243:NAD; D004317:Doxorubicin; C024555:ferric chloride