title: Localisation of androgen receptors in dermal fibroblasts, grown in vitro, from normal subjects and from patients with androgen insensitivity syndrome.
abstract: Human genital skin fibroblasts show the androgen receptor (AR) as bright red granules (0.5 micron in diameter) after treatment with one (of 3) primary polyclonal antibody and a secondary antibody linked to alkaline phosphatase. Almost all AR were cytoplasmic in 12 normal strains and in 8 strains from patients with partial (PAIS) and 6 with complete androgen insensitivity syndrome (CAIS). When 25.8 nM 5-alpha-dihydrotestosterone (DHT) or methyltrienolone was added to the medium, some AR were translocated into the nucleus in 13 experiments with 8 strains of normal fibroblasts; of 8 strains of patients with PAIS 3 failed to translocate to a detectable extent in the presence of androgen in the medium and none of the 6 strains from patients with CAIS translocated.
authors.affiliation: University Department of Paediatrics, Addenbrooke's Hospital, Cambridge, UK.
authors.forename: W
authors.initials: W
authors.lastname: Jacobson
authors.forename: J
authors.initials: J
authors.lastname: Routledge
authors.forename: H
authors.initials: H
authors.lastname: Davies
authors.forename: T
authors.initials: T
authors.lastname: Saich
authors.forename: I
authors.initials: I
authors.lastname: Hughes
authors.forename: A
authors.initials: A
authors.lastname: Brinkmann
authors.forename: B
authors.initials: B
authors.lastname: Brown
authors.forename: P
authors.initials: P
authors.lastname: Clarkson
mesh_terms: D000728:Androgens / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D001360:Avidin; D002478:Cells, Cultured; D003470:Culture Media; D004700:Endocrine System Diseases / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D011944:Receptors, Androgen / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism*
chemical_list: D000728:Androgens; D003470:Culture Media; D011944:Receptors, Androgen; D018160:Receptors, Cytoplasmic and Nuclear; D001360:Avidin