[A study of the relationship between expression of IGF-II, IGF-IIR, HBxAg and the DNA ploidy, cell cycle of hepatocytes in hepatocarcinoma].
In order to study the role of insulin-like growth factor II (IGF-II) in the development of hepatocarcinoma (HCC), the expression of IGF-II, IGF-II receptor (IGF-IIR) and HBxAg in HCC was studied with immunohistochemistry (PAP method). Meanwhile DNA ploidy and S-phase fraction of hepatocytes were analyzed with flow cytometry. The results were as follows: (1) IGF-II, IGF-IIR and HBxAg showed positive staining simultaneously in the tumor tissues of 93% (n = 15) of the HCC cases with chronic liver disease and with positive evidence of HBV; (2) The mean S-phase incidence in tissues of IGF-II positive HCC was 28.6 +/- 6.4%; this was higher than 12.8 +/- 2.4% in the IGF-II negative tumors (P < 0.05); (3) The incidence of DNA-aneuploidy in IGF-II positive liver tissues was 100% (10/10); this was higher than 60% (6/10) in IGF-II negative liver tissues (P < 0.05). It is suggested that IGF-II might play an important role in the development of HCC when there is evidence of HBV and chronic liver disease involvement. IGF-II positive staining HCC have increased proliferative activity as compared with IGF-II negative staining tumors.
Deng
T
T
Institute of Liver Disease, PLA, Beijing.
Chen
N L
NL
Jia
K M
KM
D000328:Adult; D000368:Aged; D000782:Aneuploidy; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000235:genetics / Q000821:virology; D004273:DNA, Neoplasm / Q000378:metabolism*; D005434:Flow Cytometry; D006509:Hepatitis B / Q000276:immunology; D006511:Hepatitis B Antigens / Q000378:metabolism; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D008113:Liver Neoplasms / Q000737:chemistry / Q000235:genetics / Q000821:virology; D008875:Middle Aged; D017527:Receptor, IGF Type 2 / Q000378:metabolism*; D016196:S Phase; D015534:Trans-Activators / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins
D004273:DNA, Neoplasm; D006511:Hepatitis B Antigens; D017527:Receptor, IGF Type 2; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D007335:Insulin-Like Growth Factor II