Identification of Acan125 as a myosin-I-binding protein present with myosin-I on cellular organelles of Acanthamoeba.
We have discovered the first protein to bind to a non-filamentous myosin, aside from actin. This protein, Acan125, is a 125-kDa protein from Acanthamoeba that associates with the SH3 domain of Acanthamoeba myosin-IC and not the SH3 domain of human fodrin. Antibodies raised against Acan125 recognize a single protein of 125 kDa from a whole cell lysate of Acanthamoeba; antibodies to myosin-I (M1.7 and M1.8) do not recognize Acan125 on the same blot. Double labeling of Acanthamoeba show Acan125 and myosin-I to be present on the same intracellular organelle, most likely amoebastomes. Immunoprecipitation with either anti-myosin-I or anti-Acan125 antibodies coprecipitates both Acan125 and myosin-I from a lysate of Acanthamoeba, demonstrating that Acan125 interacts with native myosin-I.
Xu
P
P
Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235-9040, USA.
Zot
A S
AS
Zot
H G
HG
D000048:Acanthamoeba / Q000737:chemistry*; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000276:immunology / Q000378:metabolism*; D002451:Cell Compartmentation; D002846:Chromatography, Affinity; D005455:Fluorescent Antibody Technique; D008969:Molecular Sequence Data; D009218:Myosins / Q000378:metabolism*; D015388:Organelles / Q000378:metabolism*; D011233:Precipitin Tests; D011485:Protein Binding; D015800:Protozoan Proteins / Q000276:immunology / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D018909:src Homology Domains
C096372:Acan125 protein, Acanthamoeba; D002352:Carrier Proteins; D015800:Protozoan Proteins; D011993:Recombinant Fusion Proteins; D009218:Myosins