EPR study of the sea anemone cytolysin, equinatoxin II, cytotoxicity on V-79 cells.
Electron paramagnetic resonance (EPR) was used to study the effect of equinatoxin II (EqT II), a cytolytic protein isolated from the sea anemone Actinia equina L., on membrane fluidity and cell metabolism of V-79 cells; the reduction of the spin probe incorporated into the cell membranes as well as the oxygen consumption in the cell suspension were measured. The results were compared with the results obtained by the cell viability study. Under the influence of EqT II (less than 37.5 micrograms/10(6) cells) no significant changes in cell membrane fluidity were observed, while reduction kinetics of the spin probe and the oxygen consumption decreased when the cells were kept in Tris buffer solution. However, in the presence of 10% fetal calf serum, which prevented cell lysis, the effects of EqT II were diminished. The oxygen consumption corresponds to the cell viability changes but the reduction kinetics alterations indicate that some oxidation-reduction processes other than cell respiration are affected by EqT II in the absence of serum. The effect seems to be indirect, probably due to the formation of pores which are associated with changed permeability of plasmalemma for metabolites and ions.
Batista
U
U
Institute of Biophysics, Medical Faculty, University of Ljubljana, Slovenia.
Sentjurc
M
M
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D003064:Cnidarian Venoms / Q000633:toxicity*; D006224:Cricetinae; D004578:Electron Spin Resonance Spectroscopy; D005347:Fibroblasts / Q000187:drug effects; D007700:Kinetics; D008560:Membrane Fluidity / Q000187:drug effects*; D008928:Mitochondria / Q000378:metabolism; D009589:Nitrogen Oxides / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption / Q000187:drug effects; D012615:Sea Anemones; D013113:Spin Labels
D003064:Cnidarian Venoms; D009589:Nitrogen Oxides; D013113:Spin Labels; C012307:equinatoxin