Local synthesis of apolipoprotein J in the eye.
Apolipoprotein J (apoJ), a secretory glycoprotein known to transport lipids and to regulate terminal complement function, is present in the human eye in both aqueous and vitreous, as well as in the retina. Ocular apoJ is the product of local synthesis, rather than plasma contamination, as demonstrated by its distinct structural properties and the presence of abundant apoJ mRNA in retina and retina pigment epithelium. ApoJ mRNA is also present in mouse eye, with a developmentally regulated pattern of expression. In fetal mouse, apoJ mRNA is present in retina, lens and cornea. In contrast, adult eye apoJ mRNA is present in retina and ciliary body. We propose that apoJ is important in tissue remodeling and in stabilizing hydrophobic molecules which are required for vision and/or which would otherwise be deleterious and membrane-active.
Reeder
D J
DJ
Department of Ophthalmology, University of Cincinnati College of Medicine, OH, USA.
Stuart
W D
WD
Witte
D P
DP
Brown
T L
TL
Harmony
J A
JA
D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000737:chemistry; D051152:Clusterin; D003169:Complement Inactivator Proteins / Q000032:analysis / Q000096:biosynthesis*; D015180:Electrophoresis, Gel, Two-Dimensional; D005123:Eye / Q000378:metabolism*; D005136:Eye Proteins / Q000032:analysis; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones; D014822:Vitreous Body / Q000737:chemistry
C495242:CLU protein, human; D051152:Clusterin; D003169:Complement Inactivator Proteins; D005136:Eye Proteins; D006023:Glycoproteins; D018832:Molecular Chaperones