Interleukin-4 stimulates pro-alpha 1(VI) collagen gene expression in cultured human osteoblast-like cells.
Interleukin-4 (IL-4) is a pleiotropic immunomodulatory cytokine produced mainly by T lymphocyte. It is known that many kinds of non-haematopoietic cells, as well as immune cells and haematopoietic cells, express IL-4 receptors. Recently, we found that IL-4 increased hydroxyproline and osteocalcin accumulation and caused mineralization in cultured human osteoblast-like cells. To clarify the mechanism of those effects of IL-4, we explored mRNAs whose expression levels were increased or decreased by IL-4 treatment using the method of differential display. As a result, expression of pro-alpha 1(VI) collagen gene in osteoblast-like cells proved to be substantially enhanced by IL-4 treatment. Considering that type VI collagen binds many kinds of extracellular matrices, type VI collagen probably mediates IL-4-dependent increase of accumulation of hydroxyproline and osteocalcin in cell layers of osteoblast-like cells.
Ishibashi
H
H
Department of Orthopaedics, University of Tokyo, Japan.
Karube
S
S
Yamakawa
A
A
Koshihara
Y
Y
D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D003001:Cloning, Molecular; D015870:Gene Expression; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011347:Procollagen / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA
D011347:Procollagen; D012333:RNA, Messenger; D015847:Interleukin-4