bcl-1, bcl-2, p53, c-myc, and lyt-10 analysis in cutaneous lymphomas.
In the present study we investigated the pathogenetic role of c-myc, bcl-2, and lyt-10 oncogenes, bcl-1 locus, and p53 suppressor gene in a representative panel of cutaneous lymphomas, including 25 cases of cutaneous B cell lymphoma (CBCL) and 29 cases of cutaneous T cell lymphoma (CTCL). In our analysis four cases of CBCL were found rearranged for bcl-2 and two for the bcl-1 locus. Two cases of CTCL and one case of CBCL were found rearranged for lyt-10. No rearrangements of c-myc oncogene were found in CBCL. Analysis of p53 gene showed mutation only in one case of mycosis fungoides in tumoral stage, at codon 163 of p53 gene (TAC-->CAC; Tyr--> Asp). Our data suggest that in primary CBCL bcl-2 oncogenes and bcl-1 locus are rarely involved. Furthermore, in primary CTCL p53 gene is not affected at significant frequency. The occurrence of p53 mutation in a patient affected by mycosis fungoides in tumoral stage may represent an involvement of p53 gene in tumor progression of CTCL, a finding observed in several types of human cancer.
Garatti
S A
SA
Laboratorio di Ematologia Sperimentale e Genetica molecolare, Istituto di Scienze Mediche, Milan, Italy.
Roscetti
E
E
Trecca
D
D
Fracchiolla
N S
NS
Neri
A
A
Berti
E
E
D001483:Base Sequence; D002869:Chromosome Aberrations; D019938:Cyclin D1; D004252:DNA Mutational Analysis; D004273:DNA, Neoplasm / Q000235:genetics*; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D015329:Gene Rearrangement, T-Lymphocyte; D005803:Genes, Immunoglobulin; D016259:Genes, myc; D016158:Genes, p53; D006801:Humans; D016393:Lymphoma, B-Cell / Q000145:classification / Q000235:genetics* / Q000473:pathology; D016410:Lymphoma, T-Cell, Cutaneous / Q000145:classification / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D009182:Mycosis Fungoides / Q000235:genetics; D016328:NF-kappa B / Q000235:genetics; D052003:NF-kappa B p52 Subunit; D009857:Oncogenes; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011518:Proto-Oncogene Proteins / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics; D012878:Skin Neoplasms / Q000235:genetics* / Q000473:pathology
D004273:DNA, Neoplasm; D016328:NF-kappa B; D052003:NF-kappa B p52 Subunit; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D019938:Cyclin D1