title: Purification and characterization of two restriction endonucleases isolated from Phormidium inundatum.
abstract: We have isolated two restriction endonucleases, PinBI and PinBII, from the cyanobacterial strain Phormidium inundatum, and identified them as isoschizomers of AvaIII and BspMII, respectively.
authors.affiliation: Department of Microbiology, University of Gdańsk, Poland.
authors.forename: S
authors.initials: S
authors.lastname: Piechula
authors.forename: J
authors.initials: J
authors.lastname: Kur
authors.forename: J
authors.initials: J
authors.lastname: Woszczyk
authors.forename: A J
authors.initials: AJ
authors.lastname: Podhajska
mesh_terms: D010582:Bacteriophage lambda / Q000378:metabolism; D001483:Base Sequence; D000458:Cyanobacteria / Q000201:enzymology*; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism*; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000302:isolation & purification; D013379:Substrate Specificity
chemical_list: D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes; C048503:ATGCAT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C053919:TCCGGA-specific type II deoxyribonucleases