Evidence for a cholesterol-lowering gene in a French-Canadian kindred with familial hypercholesterolemia.
We describe a four-generation kindred with familial hypercholesterolemia (FH) in which two of the eight heterozygotes for a 5-kb deletion (exons 2 and 3) in the low density lipoprotein (LDL) receptor gene were found to have normal LDL-cholesterol levels. In our search for a gene responsible for the cholesterol-lowering effect in this family, we have studied variation in the genes encoding the LDL receptor, apolipoprotein (apo) B, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, apoAI-CIII-AIV, and lipoprotein lipase. The analysis showed that it was unlikely that variation in any of these genes was responsible for the cholesterol-lowering effect. Expression of the LDL receptor, as assessed in vitro with measurements of activity and mRNA levels, was similar in normo and hyperlipidemic subjects carrying the deletion. Analysis of the apo E isoforms revealed that most of the e2 allele carriers in this family, including the two normolipidemic 5-kb deletion carriers, were found to have LDL-cholesterol levels substantially lower than subjects with the other apo E isoforms. Thus, this kindred provides evidence for the existence of a gene or genes, including the apo e2 allele, with profound effects on LDL-cholesterol levels.
Sass
C
C
Hyperlipidemia and Atherosclerosis Research Group, Clinical Research Institute of Montreal, Quebec, Canada.
Giroux
L M
LM
Ma
Y
Y
Roy
M
M
Lavigne
J
J
Lussier-Cacan
S
S
Davignon
J
J
Minnich
A
A
D002784:Cholesterol / Q000097:blood / Q000235:genetics*; D002872:Chromosome Deletion; D004247:DNA / Q000032:analysis; D005260:Female; D005602:France / Q000208:ethnology; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000235:genetics*; D008875:Middle Aged; D010375:Pedigree; D011973:Receptors, LDL / Q000235:genetics
D011973:Receptors, LDL; D004247:DNA; D002784:Cholesterol