Related organelles of the endosome-lysosome system contain a different repertoire of ubiquitinated proteins in Sf9 insect cells.
Two components of the endosomal/lysosomal compartment of Sf9 cells, multivesicular bodies (MVB) and light vacuoles with membrane complexes (LVMC) have been isolated and probed for ubiquitin protein conjugates with a specific antibody. Immunogold electron microscopy indicates that whereas ubiquitin-protein conjugates are localised to electron dense areas of MVB they are associated with the membranes of LVMC. Five ubiquitinated polypeptides are revealed in MVB by immunoblotting while numerous ubiquitinated species forming a smear following electrophoresis are present in LVMC. We suggest two possible routes for entry of ubiquitin-protein conjugates into these organelles, via the cell surface and via primary lysosomes.
Löw
P
P
Department of General Zoology, Eötvös University, Budapest, Hungary.
Doherty
F J
FJ
Fellinger
E
E
Sass
M
M
Mayer
R J
RJ
László
L
L
D000818:Animals; D002460:Cell Line; D011992:Endosomes / Q000737:chemistry*; D008247:Lysosomes / Q000737:chemistry*; D016253:Microscopy, Immunoelectron; D015388:Organelles / Q000737:chemistry*; D011506:Proteins / Q000032:analysis; D018411:Spodoptera; D013347:Subcellular Fractions; D014452:Ubiquitins / Q000032:analysis*
D011506:Proteins; D014452:Ubiquitins