title: SacNI, an isoschizomer of BanII isolated from Streptomyces achromogenes recognizes the 5'-GRGCY/C sequence.
abstract: SacNI, an isoschizomer of the restriction endonuclease, BanII [Sugisaki et al., Nucleic Acids Res. 10 (1982) 5747-5752], has been isolated from Streptomyces achromogenes N-J-H. SacNI recognizes the palindromic sequence, 5'-GRGCY/C, and cleaves within the recognition sequence, generating a 3' protruding RGCY end (where R = A or G, and Y = C or G).
authors.affiliation: Department of Microbiology, University of Gdańsk, Poland.
authors.forename: S M
authors.initials: SM
authors.lastname: Rutkowska
authors.forename: P M
authors.initials: PM
authors.lastname: Skowron
authors.forename: K
authors.initials: K
authors.lastname: Bielawski
authors.forename: A J
authors.initials: AJ
authors.lastname: Podhajska
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity
chemical_list: D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific; C051481:GRGCYC-specific type II deoxyribonucleases