Mechanism of the enhancing effect of sorbitol on ileal Ca uptake in rat enterocytes.
The effect of sorbitol on Ca uptake by isolated ileal epithelial cells was investigated. Intestinal cells were isolated from rat ileum by mechanical vibration. 45Ca uptake was approximately 2 times higher in cells exposed to 200 mM sorbitol of D-alanine than in control cells. This enhancing effect of sorbitol on percentage Ca uptake decreased with increasing Ca concentrations in the incubation medium suggesting an effect on Ca entry velocity. The addition of 10 microM nifedipine or 200 microM verapamil to the incubation medium was devoid of any effect on Ca uptake in ileal cells, whereas 100 microM trifluoperazine or chlorpromazine abolished the stimulatory effect of sorbitol. Finally, the effect of sorbitol on isolated cells was independent of a measurable change of cellular ATP content. In conclusion, the stimulatory effect of sorbitol on ileal Ca uptake is probably exerted through mechanisms other than an increase in intracellular ATP concentration. Sorbitol may enhance enterocyte Ca transport via a direct interaction with calmodulin and/or the Ca pump. It may also exert its effect through an inhibition of the basolateral Na Ca exchanger.
Tardivel
S
S
Métabolisme Minéral des Mammifères, EPHE, Faculté de Pharmacie, Chatenay Malabry, France.
Razanamaniraka
L
L
Drüeke
T
T
Lacour
B
B
D000255:Adenosine Triphosphate; D000409:Alanine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002746:Chlorpromazine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007082:Ileum / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013012:Sorbitol / Q000494:pharmacology*; D013997:Time Factors; D014268:Trifluoperazine / Q000494:pharmacology
D014268:Trifluoperazine; D013012:Sorbitol; D000255:Adenosine Triphosphate; D000409:Alanine; D002118:Calcium; D002746:Chlorpromazine