The cytotoxic T lymphocyte gene FIBLP with homology to fibrinogen beta and gamma subunits is also induced in mouse macrophages by IFN-gamma.
To identify genes induced in mouse macrophages by IFN-gamma, a cDNA subtraction library of IFN-gamma-induced genes was screened. One of the clones, 36F2, was identified by DNA sequencing as the FIBLP gene. The FIBLP (fibrinogen-like protein) gene is a T-lymphocyte-specific gene that is expressed in mouse cytotoxic T lymphocytes but not in helper T lymphocytes or B lymphocytes. The protein sequence shows a high homology to fibrinogen beta and gamma subunits. The FIBLP gene is not expressed in unstimulated mouse peritoneal macrophages but is induced by IFN-gamma to high levels. FIBLP mRNA is detected by 1 hr after the addition of IFN-gamma and maximal levels are reached by 12 hr. Expression of FIBLP mRNA was not induced by IL-2, IL-4, IL-10, or TNF-alpha. Though the function of this gene is unknown, its expression in both cytotoxic T lymphocytes and activated macrophages suggests that FIBLP may play an as yet undefined role in the cytotoxic function of these cells.
Lafuse
W P
WP
Department of Medical Microbiology and Immunology, Ohio State University, Columbus 43210, USA.
Castle
L
L
Brown
D
D
Zwilling
B S
BS
D000818:Animals; D004247:DNA; D005340:Fibrinogen / Q000037:antagonists & inhibitors / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology; D007371:Interferon-gamma / Q000494:pharmacology*; D007378:Interleukins / Q000502:physiology; D017737:Macrophages, Peritoneal / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D012689:Sequence Homology, Nucleic Acid; D013602:T-Lymphocytes, Cytotoxic / Q000502:physiology*
D007378:Interleukins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D007371:Interferon-gamma; D005340:Fibrinogen; D004247:DNA