Preferential reduction of nm23-H1 gene product in metastatic tissues from papillary and follicular carcinomas of the thyroid.
The nm23 gene product is a possible mediator of cancer invasion and metastasis, and has been divided into two distinct gene products, NM23-H1 and NM23-H2. It has been shown previously that expression of nm23-H1 messenger RNA is reduced in metastatic lymph nodes from patients with papillary carcinoma of the thyroid (Am J Pathol 142:1938-1944, 1993). Since mAb H1-229 specific for NM23-H1 is now available, we further extended our study to examine the expression of nm23-H1 gene product in various types of thyroid cancer (37 cases of papillary carcinoma, 42 cases of follicular carcinoma, 17 cases of anaplastic carcinoma, and 3 cases of medullary carcinoma). NM23-H1 was expressed in primary papillary and follicular carcinomas (14/15 and 21/22), but weakly or hardly expressed in metastatic lymph nodes and metastatic bone marrow (6/23 and 1/4) (P < 0.01). Expression of NM23-H1 was low or absent in anaplastic and medullary carcinoma (3/17 and 0/3) (P < 0.01).
Arai
T
T
Department of Pathology, Saitama Medical Center, Saitama Medical School, Kawagoe, Japan.
Yamashita
T
T
Urano
T
T
Masunaga
A
A
Itoyama
S
S
Itoh
K
K
Shiku
H
H
Sugawara
I
I
D018263:Adenocarcinoma, Follicular / Q000378:metabolism* / Q000556:secondary; D000911:Antibodies, Monoclonal; D001853:Bone Marrow / Q000378:metabolism; D018276:Carcinoma, Medullary / Q000378:metabolism* / Q000556:secondary; D002291:Carcinoma, Papillary / Q000378:metabolism* / Q000556:secondary; D006801:Humans; D015151:Immunoblotting; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000378:metabolism; D008207:Lymphatic Metastasis; D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase / Q000096:biosynthesis; D013964:Thyroid Neoplasms / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000096:biosynthesis*
D000911:Antibodies, Monoclonal; D054778:NM23 Nucleoside Diphosphate Kinases; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins