FimA, a major virulence factor associated with Streptococcus parasanguis endocarditis.
Adherence of microorganisms to damaged heart tissue is a crucial event in the pathogenesis of infective endocarditis. In the present study, we investigated the role of the FimA protein as a potential virulence factor associated with Streptococcus parasanguis endocarditis. FimA is a 36-kDa surface protein that is a recognized adhesin in the oral cavity where it mediates adherence to the salivary pellicle. An insertion mutant and a deletion mutant of S. parasanguis were employed in the rat model of endocarditis to determine the relevance of FimA in endocarditis pathogenesis. Catheterized rats were infected with either the fimA deletion mutant VT929, the fimA insertion mutant VT930, or the isogenic, wild-type S. parasanguis FW213. Rats inoculated with FW213 developed endocarditis more frequently (50.9%) than animals inoculated with either the deletion mutant (2.7%) or the insertion mutant (7.6%) (P < 0.001). A series of in vitro assays were performed to explore the mechanism(s) by which FimA enhanced the infectivity of S. parasanguis. FimA did not inhibit the uptake or the subsequent killing of S. parasanguis by phagocytic granulocytes. Similarly, FimA did not play a role in the adherence to or the aggregation of platelets. Significant differences were noted between FW213 and VT929 (P < 0.05) and FW213 and VT930 (P < 0.001) in their abilities to bind to fibrin monolayers. The mean percent adherence of FW213 to fibrin monolayers (2.1%) was greater than those of VT929 (0.5%) and VT930 (0.12%). Taken together, these results indicate that FimA is a major virulence determinant associated with S. parasanguis endocarditis and further suggest that its role is associated with initial colonization of damaged heart tissue.
Burnette-Curley
D
D
Department of Microbiology and Immunology, Medical College of Virginia-Virginia Commonwealth University, Richmond 23298, USA.
Wells
V
V
Viscount
H
H
Munro
C L
CL
Fenno
J C
JC
Fives-Taylor
P
P
Macrina
F L
FL
D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D044622:Dental Pellicle; D004697:Endocarditis, Bacterial / Q000209:etiology*; D036781:Fimbriae Proteins; D006801:Humans; D008297:Male; D009154:Mutation; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013290:Streptococcal Infections / Q000209:etiology*; D013291:Streptococcus / Q000472:pathogenicity; D014774:Virulence
D001426:Bacterial Proteins; C063055:fimbrillin; D036781:Fimbriae Proteins