Sequence analysis of HIV-1 vif gene in Spanish isolates.
We have determined the nucleotide sequence of the HIV-1 vif gene in viruses obtained from symptomatic patients of distinct risk groups in Madrid. The genetic diversity among the isolates was estimated in 4.6% (+/- 1.4 standard deviation), a similar value to that obtained for the gag gene 3.9% (+/- 0.8 standard deviation) and env 4.1% (+/- 1 standard deviation) (Rojas et al., Virus Res 31, 331-342, 1994). Amino acid sequence analysis revealed the presence of hypermutable residues at positions 101 and 167, close to antigenically relevant sequential epitopes (comprising amino acids 87-94 and 172-178). Phylogenetic analysis supports the existence of two virus lineages circulating preferentially within different risk groups.
Olivares
I
I
Centro Nacional de Biología Celular y Retrovirus, Instituto Carlos III, Majadahonda, Madrid, Spain.
Menéndez-Arias
L
L
Rodríguez-Bernabé
A
A
Martin
M J
MJ
Dopazo
J
J
López-Galíndez
C
C
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D015897:Comorbidity; D016384:Consensus Sequence; D016341:Genes, vif; D014644:Genetic Variation; D015658:HIV Infections / Q000151:congenital / Q000453:epidemiology / Q000821:virology; D015497:HIV-1 / Q000235:genetics* / Q000302:isolation & purification; D006716:Homosexuality; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D010802:Phylogeny; D012307:Risk Factors; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013030:Spain / Q000453:epidemiology; D015819:Substance Abuse, Intravenous / Q000453:epidemiology; D065227:Transfusion Reaction