The Candida boidinii peroxisomal membrane protein Pmp30 has a role in peroxisomal proliferation and is functionally homologous to Pmp27 from Saccharomyces cerevisiae.
The mechanism of peroxisome proliferation is poorly understood. Candida boidinii is a methylotrophic yeast that undergoes rapid and massive peroxisome proliferation and serves as a good model system for this process. Pmp30A and Pmp30B (formerly designated Pmp31 and Pmp32, respectively) are two closely related proteins in a polyploid strain of this yeast that are strongly induced by diverse peroxisome proliferators such as methanol, oleate, and D-alanine. The function of these proteins is not understood. To study this issue, we used a recently described haploid strain (S2) of C. boidinii that can be manipulated genetically. We now report that strain S2 contains a single PMP30 gene very similar in sequence (greater than 93% identity at the DNA level) to PMP30A and PMP30B. When PMP30 was disrupted, cell growth on methanol was greatly inhibited, and cells grown in both methanol and oleate had fewer, larger, and more spherical peroxisomes than wild-type cells. A similar phenotype was recently described for Saccharomyces cerevisiae cultured on oleate in which PMP27, which encodes a protein of related sequence that is important for peroxisome proliferation, was disrupted. To determine whether Pmp27 is a functional homolog of Pmp30, gentle complementation was performed. PMP30A was expressed in the PMP27 disruptant of S. cerevisiae, and PMP27 was expressed in the PMP30 disruptant of C. boidinii S2. Complementation, in terms of both cell growth and organelle size, shape, and number, was successful in both directions, although reversion to a wild-type phenotype was only partial for the PMP30 disruptant. We conclude that these proteins are functional homologs and that both Pmp30 and Pmp27 have a direct role in proliferation and organelle size rather than a role in a specific peroxisomal metabolic pathway of substrate utilization.
Sakai
Y
Y
Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Japan.
Marshall
P A
PA
Saiganji
A
A
Takabe
K
K
Saiki
H
H
Kato
N
N
Goodman
J M
JM
D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D006238:Haploidy; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D008830:Microbodies / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D019301:Oleic Acid; D009829:Oleic Acids / Q000378:metabolism; D000074401:Peroxins; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D005656:Fungal Proteins; D008565:Membrane Proteins; D009829:Oleic Acids; C092128:PEX11 protein, S cerevisiae; C092216:PMP31 protein, Candida boidinii; C092217:PMP32 protein, Candida boidinii; D000074401:Peroxins; D029701:Saccharomyces cerevisiae Proteins; D019301:Oleic Acid; D000432:Methanol