title: Isolation of Japanese encephalitis virus from mosquitoes collected in Sabak Bernam, Selangor, Malaysia in 1992.
abstract: Detection and isolation of Japanese encephalitis (JE) virus were attempted from female mosquitoes collected in Kampong Pasir Panjang, Sabak Bernam, Selangor, from May to November 1992. A total of 7,400 mosquitoes consisting of 12 species in 148 pools were processed and inoculated into Aedes albopictus clone C6/36 cell cultures. Of these, 26 pools showed the presence of viral antigens in the infected C6/36 cells by specific immunoperoxidase staining using an anti-JE virus polyclonal antibody. Presence of JE virus genome was confirmed in the infected culture fluid for 16 pools by using reverse transcriptase-polymerase chain reaction and JE virus-specific primers. Of these, 3 pools were from Culex tritaeniorhynchus, 4 from Culex vishnui, 3 from Culex bitaeniorhynchus, 2 from Culex sitiens, one from Aedes species, and 3 from Culex species. Isolation of JE virus from Cx. sitiens, Cx. bitaeniorhynchus, and Aedes sp. (Aedes butleri and Ae. albopictus) is reported for the first time in Malaysia.
authors.affiliation: Institute for Medical Research, Kuala Lumpur, Malaysia.
authors.forename: I
authors.initials: I
authors.lastname: Vythilingam
authors.forename: K
authors.initials: K
authors.lastname: Oda
authors.forename: T K
authors.initials: TK
authors.lastname: Chew
authors.forename: S
authors.initials: S
authors.lastname: Mahadevan
authors.forename: B
authors.initials: B
authors.lastname: Vijayamalar
authors.forename: K
authors.initials: K
authors.lastname: Morita
authors.forename: H
authors.initials: H
authors.lastname: Tsuchie
authors.forename: A
authors.initials: A
authors.lastname: Igarashi
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001483:Base Sequence; D002460:Cell Line; D003465:Culex / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D008296:Malaysia; D008969:Molecular Sequence Data
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral