A simple HPLC method for the isolation and quantification of the allergens tuliposide A and tulipalin A in Alstroemeria.
A practical, rapid, reliable and sensitive method for the isolation and determination of the allergens tuliposide A and alpha-methylene-gamma-butyrolactone (tulipalin A), by reversed-phase high-performance liquid chromatography (RP-HPLC), has been developed in order to select Alstroemeria species for breeding purposes. From the aqueous extracts of flowers, stems and leaves, of several Alstroemeria species, the contents of 6-tuliposide A and tulipalin A were determined by isocratic RP-HPLC, using distilled water as mobile phase. The compounds were detected by an UV detector at 208 nm. Differences in 6-tuliposide A and tulipalin A content were found among the species investigated, with the highest concentrations in stems and flowers. The absence of other tuliposides (e.g., 1-tuliposide A, 1- and 6-tuliposide B) in extracts was proven by TLC, RP-HPLC, 1H- and 13C-NMR. 6-Tuliposide A and tulipalin A were identified by 1H- and 13C-NMR and comparison with authentic material, respectively. With this HPLC method, it is possible to investigate a large number of plants for their contents of tuliposide A and tulipalin A, within a minimum of time, and to isolate them directly from aqueous extracts.
Christensen
L P
LP
Department of Ornamentals, Research Center Arslev, Danish Institute of Plant and Soil Science.
Kristiansen
K
K
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000302:isolation & purification; D000485:Allergens / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003877:Dermatitis, Contact / Q000209:etiology; D006027:Glycosides / Q000302:isolation & purification*; D006801:Humans; D010944:Plants / Q000737:chemistry*; D012978:Sodium Oxybate / Q000031:analogs & derivatives* / Q000302:isolation & purification
D000485:Allergens; D006027:Glycosides; C062516:tuliposide A; C014096:alpha-methylene gamma-butyrolactone; D012978:Sodium Oxybate; D015107:4-Butyrolactone