title: Meso1, a basic-helix-loop-helix protein involved in mammalian presomitic mesoderm development.
abstract: To identify genes involved in the regulation of early mammalian development, we have developed a dominant-negative mutant basic-helix-loop-helix (bHLH) protein probe for interaction cloning and have isolated a member of the bHLH family of transcription factors, Meso1. Meso1-E2A heterodimers are capable of binding to oligonucleotide probes that contain a bHLH DNA recognition motif. In mouse embryos, Meso1 is expressed prior to MyoD1 family members. Meso1 expression is first detected at the neural plate stage of development in the paraxial mesoderm of the head and in presomitic mesodermal cells prior to their condensation into somites. Our findings suggest that Meso1 may be a key regulatory gene involved in the early events of vertebrate mesoderm differentiation.
authors.affiliation: Hormone Research Institute, University of California, San Francisco 94143, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Blanar
authors.forename: P H
authors.initials: PH
authors.lastname: Crossley
authors.forename: K G
authors.initials: KG
authors.lastname: Peters
authors.forename: E
authors.initials: E
authors.lastname: Steingrímsson
authors.forename: N G
authors.initials: NG
authors.lastname: Copeland
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: G R
authors.initials: GR
authors.lastname: Martin
authors.forename: W J
authors.initials: WJ
authors.lastname: Rutter
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003433:Crosses, Genetic; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D005314:Embryonic and Fetal Development; D005260:Female; D018257:Helix-Loop-Helix Motifs; D017403:In Situ Hybridization; D007668:Kidney / Q000378:metabolism; D008297:Male; D008648:Mesoderm / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009115:Muridae; D018482:Muscle, Skeletal / Q000378:metabolism; D017570:MyoD Protein / Q000096:biosynthesis / Q000235:genetics; D009206:Myocardium / Q000378:metabolism; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013737:Testis / Q000378:metabolism; D015534:Trans-Activators / Q000096:biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D017570:MyoD Protein; D012333:RNA, Messenger; D015534:Trans-Activators; D014157:Transcription Factors; C094319:Meso1 protein, Mesocricetus auratus; C057363:MyoD1 myogenic differentiation protein