Evidence of a cadmium-thionein and the glycine cleavage system in Oxytricha granulifera.
This work presents the further purification of a Cd-linking protein in Oxytricha granulifera by reverse-phase chromatography. This protein contains 25% cysteine and no aromatic amino acid. It may be considered as a chelatin with some similarity to metallothioneins. During the purification of another Cd-linking compound, we were able to demonstrate that the H protein precursor of glycine cleavage is present in Oxytricha. This is the first finding of the presence of this system in Protozoa.
Irato
P
P
Dipartimento di Biologia, Università di Padova, Italy.
Piccinni
E
E
James
P
P
Ammermann
D
D
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002104:Cadmium / Q000378:metabolism*; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005998:Glycine / Q000378:metabolism*; D006801:Humans; D008668:Metallothionein / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016813:Oxytricha / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
D000596:Amino Acids; C004203:cadmium-binding protein; D002104:Cadmium; D008668:Metallothionein; D005998:Glycine