Cloning and characterization of the Streptomyces peucetius dnrQS genes encoding a daunosamine biosynthesis enzyme and a glycosyl transferase involved in daunorubicin biosynthesis.
The dnrQS genes from the daunorubicin producer Streptomyces peucetius were characterized by DNA sequencing, complementation analysis, and gene disruption. The dnrQ gene is required for daunosamine biosynthesis, and dnrS appears to encode a glycosyltransferase for the addition of the 2,3,6-trideoxy-3-aminohexose, daunosamine, to epsilon-rhodomycinone.
Otten
S L
SL
School of Pharmacy, University of Wisconsin, Madison 53706, USA.
Liu
X
X
Ferguson
J
J
Hutchinson
C R
CR
D000595:Amino Acid Sequence; D018943:Anthracyclines / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System; D003630:Daunorubicin / Q000096:biosynthesis*; D005798:Genes, Bacterial / Q000235:genetics*; D005816:Genetic Complementation Test; D016695:Glycosyltransferases; D006595:Hexosamines / Q000096:biosynthesis* / Q000378:metabolism; D008956:Models, Chemical; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*
D018943:Anthracyclines; D001426:Bacterial Proteins; C096749:DnrQ protein, Streptomyces peucetius; D006595:Hexosamines; C015490:rhodomycinone; C007217:daunosamine; D003577:Cytochrome P-450 Enzyme System; D016695:Glycosyltransferases; C096750:DnrS protein, Streptomyces peucetius; D003630:Daunorubicin