title: Glycosylated human prolactin: detection by immunoblotting of biological fluids is confounded by antisera crossreactivity with immunoglobulins.
abstract: Glycosylated human prolactin is found in several biological fluids and can only be detected by western immunoblotting. Glycosylated prolactin migrates at 25,000-27,000 molecular weight while prolactin migrates at 23,000. We have discovered that several anti-human prolactin sera crossreact with some light chain components of human immunoglobulins, which also migrate at 25,000 on electrophoretic gels run under reducing conditions. Evidence is provided for prolactin binding to immunoglobulins in biological fluids, which may explain why some polyclonal anti-prolactin sera demonstrate this crossreactivity.
authors.affiliation: Department of Obstetrics and Gynecology, Brigham and Women's Hospital, Boston, MA 02115, USA.
authors.forename: L J
authors.initials: LJ
authors.lastname: Heffner
authors.forename: E
authors.initials: E
authors.lastname: Markoff
authors.forename: R W
authors.initials: RW
authors.lastname: Yuan
authors.forename: D W
authors.initials: DW
authors.lastname: Lee
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry; D000818:Animals; D016477:Artifacts; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D011247:Pregnancy; D011388:Prolactin / Q000031:analogs & derivatives* / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins; C043928:prolactin, glycosylated; D011388:Prolactin