title: Section directed cryosectioning of specimens for scanning electron microscopy: a new method to study cardiac development.
abstract: A new method to study the developing heart was developed. Using this "section directed" cryosectioning method, appropriate fixed embryos can be trimmed optimally to obtain sectional planes that, if necessary, can be matched with histologically treated sections. As a result, the morphological information obtained with the scanning electron microscope can be compared in detail with the information on the molecular phenotypes of the subpopulations of cells as deducted from staining patterns of the sections. This method allows combination of the specific advantages of sophisticated histological techniques, such as immunohistochemistry and in situ hybridisation, with those of the scanning electron microscope.
authors.affiliation: Department of Pathology, Seoul National University of College of Medicine, Korea.
authors.forename: J W
authors.initials: JW
authors.lastname: Seo
authors.forename: E K
authors.initials: EK
authors.lastname: Kim
authors.forename: N A
authors.initials: NA
authors.lastname: Brown
authors.forename: A
authors.initials: A
authors.lastname: Wessels
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D003402:Creatine Kinase / Q000032:analysis; D016616:Cryoultramicrotomy / Q000379:methods*; D006321:Heart / Q000196:embryology*; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008855:Microscopy, Electron, Scanning; D016253:Microscopy, Immunoelectron; D009206:Myocardium / Q000737:chemistry / Q000648:ultrastructure*; D009218:Myosins / Q000032:analysis
chemical_list: D000911:Antibodies, Monoclonal; D003402:Creatine Kinase; D009218:Myosins