title: Complex formation with methylamine dehydrogenase affects the pathway of electron transfer from amicyanin to cytochrome c-551i.
abstract: Methylamine dehydrogenase (MADH), amicyanin, and cytochrome c-551i are soluble redox proteins that form a complex in solution (Chen, L., Durley, R., Mathews, F. S., and Davidson, V. L. (1994) Science 264, 86-90), which is required for the physiologic electron transfer from the tryptophan tryptophylquinone cofactor of MADH to heme via the copper center of amicyanin. The reduction of cytochrome by amicyanin within the complex in solution has been demonstrated using rapid scanning stopped-flow spectroscopy. Electron transfer from free, uncomplexed, amicyanin to cytochrome c-551i occurs much more rapidly but only to a very small extent because the reaction is thermodynamically much less favorable when amicyanin is not associated with MADH (Gray, K. A., Davidson, V. L., and Knaff, D. B. (1988) J. Biol. Chem. 263, 13987-13990). These kinetic data suggest that amicyanin binding to cytochrome c-551i occurs at different sites when amicyanin is free and when it is in complex with MADH. A model for the interactions of these proteins is presented.
authors.affiliation: Department of Biochemistry, University of Mississippi Medical Center, Jackson 39216-4505, USA.
authors.forename: V L
authors.initials: VL
authors.lastname: Davidson
authors.forename: L H
authors.initials: LH
authors.lastname: Jones
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D003574:Cytochrome c Group / Q000737:chemistry / Q000378:metabolism*; D004579:Electron Transport; D007700:Kinetics; D008667:Metalloproteins / Q000378:metabolism; D008954:Models, Biological; D008961:Models, Structural; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000737:chemistry / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D013053:Spectrophotometry; D013997:Time Factors
chemical_list: D001426:Bacterial Proteins; D003574:Cytochrome c Group; D008667:Metalloproteins; C017079:mauC protein, Methylobacterium extorquens; C017167:cytochrome C(551); C021567:methylamine dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors