[Evaluation of the usefulness of commercial systems Quantum II and Rapid ID 32E for quick identification of rods from the Enterobacteriaceae family].
The usefulness of the commercial systems Quantum II (Abbott) and Rapid ID 32 E (bioMérieux) for quick identification of Enterobacteriaceae has been evaluated. The study was carried out on strains representing 12 genera of Enterobacteriaceae identified with the aid of classical biochemical tests. The Quantum II system was evaluated in 1985 using 386 strains and the Rapid ID 32 E system in 1992 using 106 strains. When the Quantum II system was used, 237(61.4%) strains were classified correctly in the species, 58(15.0%) were unidentified and 91(23.6%) were identified incorrectly. The Rapid ID 32 E system made possible correct identification of 63(59.4%) strains to the species (in the case of Salmonella rods to the subgenus) and 80(75.5%) strains to the genus. Incorrect identification results were most often obtained in the case of Shigella and Yersinia rods, Salmonella rods of subgenus I fermenting lactose and Enterobacter. The results show that complying with inoculum tests (0.5 according to the McFarland scale) as recommended by the manufacturer and a four-hour incubation, when investigating some Enterobacteriaceae strains which multiply more slowly or exhibit a weaker metabolic activity, does not ensure optimal conditions for the appearance of a set of biochemical features essential for strain identification.
Kałuzewski
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Zakład Bakteriologii PZH w Warszawie.
D015373:Bacterial Typing Techniques; D004755:Enterobacteriaceae / Q000145:classification* / Q000302:isolation & purification