Cloning and sequence analysis of caprine N-acetylglucosamine 6-sulfatase cDNA.
Mucopolysaccharidosis IIID results from the deficiency of N-acetylglucosamine 6-sulfatase activity. A Nubian goat with this lysosomal storage disease has been identified. As a first step in developing this animal model for testing treatment methods, we cloned and sequenced the caprine N-acetylglucosamine 6-sulfatase cDNA coding region. Overall there is 88% nucleotide homology between the goat and human sequence and 94% homology of the deduced amino acid sequence. The human and two ruminant species differ by the presence of an imperfect trinucleotide (CCG) repeat in the ruminant signal sequence.
Friderici
K
K
Department of Pathology, Michigan State University, East Lansing 48824-1314, USA.
Cavanagh
K T
KT
Leipprandt
J R
JR
Traviss
C E
CE
Anson
D S
DS
Hopwood
J J
JJ
Jones
M Z
MZ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry*; D006041:Goats / Q000235:genetics*; D008969:Molecular Sequence Data; D009084:Mucopolysaccharidosis III / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013429:Sulfatases / Q000172:deficiency / Q000235:genetics*
D018076:DNA, Complementary; D013429:Sulfatases; C018719:N-acetylglucosamine-6-sulfatase