title: Quantitative measurement of HbA1c by an immunoturbidimetric assay compared to a standard HPLC method.
abstract: Determination of hemoglobin A1c (HbA1c) is one of the most important monitoring procedures for long-term control of diabetes mellitus. Several analytical methods have been developed for the measurement of glycohemoglobin (GHb). Those most frequently used are ion-exchange chromatography for HbA1c and affinity chromatography for total GHb. In this study, a new turbidimetric immunoassay for HbA1c (Boehringer Mannheim, Germany) was evaluated that was performed on a Hitachi 911 clinical chemistry analyzer (Boehringer Mannheim). Good linearity in the range of 5% to 15% HbA1c, within-run and between-run coefficients of variation ranging from 2.4% to 5.9% were obtained. Results of 179 diabetic and nondiabetic patients showed good correlation to those of a routine HPLC method (r = 0.96). In addition, HbA2, HbS, and HbF in samples from nondiabetic patients were not detected by the immunoturbimetric assay and the "labile" HbA1c fraction (Schiff base) did not interfere with the new test.
authors.affiliation: Institute for Medical and Clinical Laboratory Diagnosis, University of Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Hamwi
authors.forename: C R
authors.initials: CR
authors.lastname: Schweiger
authors.forename: M
authors.initials: M
authors.lastname: Veitl
authors.forename: R
authors.initials: R
authors.lastname: Schmid
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards*; D003920:Diabetes Mellitus / Q000097:blood; D006442:Glycated Hemoglobin / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods / Q000592:standards*; D007231:Infant, Newborn; D016014:Linear Models; D009391:Nephelometry and Turbidimetry; D015203:Reproducibility of Results
chemical_list: D006442:Glycated Hemoglobin