Recognition ability and cytotoxicity of some oligosaccharidyl substituted beta-cyclodextrins.
This paper reports a chemico-enzymatic synthesis of beta-CD derivatives. The recognition properties of these derivatives were tested using flocculating yeast and isolated lectins. It was observed that the substitution of beta-cyclodextrins with galactose end arms induces the better recognition by a cell-linked galactose-specific lectin. The physicochemical effects of the beta-CD derivatives on membranes were estimated using red blood cells and the effects on the viability of yeast and human rectal tumor cells were appreciated by measuring the mitochondrial deshydrogenase activity. The substitutions of the beta-CD ring by sugar antennae decrease the negative physicochemical effects of the beta-CD, ie their hemolytic properties. However, these substitutions induce significant modifications of the biological properties of the molecules, particularly the cytotoxicity and the growth of eukaryotic cells.
Attioui
F
F
Biochimie Microbienne, Microbiologie Moléculaire, GEVSM, Faculté des Sciences Pharmaceutiques et Biologiques, BP 403, Université Henri Poincaré, Nancy 1, France.
al-Omar
A
A
Leray
E
E
Parrot-Lopez
H
H
Finance
C
C
Bonaly
R
R
D000818:Animals; D002240:Carbohydrate Sequence; D002470:Cell Survival / Q000187:drug effects; D003505:Cyclodextrins / Q000138:chemical synthesis*; D005057:Eukaryotic Cells / Q000187:drug effects; D005428:Flocculation Tests; D006386:Hemagglutination Tests; D006801:Humans; D007716:Kluyveromyces / Q000187:drug effects / Q000254:growth & development; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development; D014407:Tumor Cells, Cultured; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D009844:Oligosaccharides; D047392:beta-Cyclodextrins; C031215:betadex