Modulation of adhesive properties of DEAE-dextran with laminin.
Human squamous epithelial cells produce lower amounts of laminin and fibronectin when cultured on DEAE-dextran than when cultured on gelatin-coated polystyrene (Biotechnol. Bioeng., 33:1235). The epithelial cells also spread much more slowly on DEAE-dextran than they do on gelatin-coated polystyrene. To determine if the low level of matrix production by cells grown on DEAE-dextran contributed to the slowness of cell spreading on this substrate, microcarriers made from DEAE-dextran were treated with exogenous laminin (10 micrograms/cm2 of surface area) and then examined for ability to support cell adhesion. Squamous epithelial cells spread as rapidly on the laminin-treated DEAE-dextran as they did on gelatin-coated polystyrene (much more rapidly than on untreated DEAE-dextran). This indicates 1) that laminin can bind to DEAE-dextran in a fashion that is biologically usable by anchorage-dependent cells, and 2) that when laminin is bound to DEAE-dextran, the failure of squamous epithelial cells to rapidly spread is overcome. These data support the hypothesis that failure of the cells to synthesize an intact extracellular matrix on DEAE-dextran is responsible, at least in part, for the slowness with which cells spread on this substrate.
Varani
J
J
Department of Pathology, University of Michigan, Ann Arbor 48109, USA.
Fligiel
S E
SE
Inman
D R
DR
Beals
T F
TF
Hillegas
W J
WJ
D000269:Adhesives; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002448:Cell Adhesion; D002460:Cell Line; D003637:DEAE-Dextran; D004847:Epithelial Cells; D005109:Extracellular Matrix / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D007797:Laminin; D008855:Microscopy, Electron, Scanning; D011137:Polystyrenes; D012878:Skin Neoplasms / Q000473:pathology; D013499:Surface Properties
D000269:Adhesives; D007797:Laminin; D011137:Polystyrenes; D003637:DEAE-Dextran