Structure, organization, and chromosomal location of the gene encoding a form of rice soluble starch synthase.
A rice (Oryza sativa L.) genomic clone encoding the gene for a form of soluble starch synthase (SSS1) and its 5'- and 3'-flanking regions has been isolated and sequenced. The SSS1 gene contained 15 exons interrupted by 14 introns. The exon/intron organization of the SSS1 gene was divergent from that of the rice Waxy gene coding for granule-bound starch synthase, thus suggesting that the SSS1 and granule-bound starch synthase genes have evolved from an ancestral gene in a different way or that the two genes are products of different ancestral genes that have converged during evolution. However, these two genes were closely located to each other on rice chromosome 6 at an approximate map distance of 5 centimorgans. The nucleotide sequence of the 5'-end region of the gene is unique because of the presence of some repetitive sequences.
Tanaka
K
K
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Ohnishi
S
S
Kishimoto
N
N
Kawasaki
T
T
Baba
T
T
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017343:Genes, Plant; D008040:Genetic Linkage; D015698:Genomic Library; D007438:Introns; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013214:Starch Synthase / Q000096:biosynthesis / Q000235:genetics*
D013214:Starch Synthase