Effect of oxidant exposure on isolated rat colonocytes.
BACKGROUND
The role of reactive oxygen species in injury to cells lining the gastrointestinal tract has gained importance in recent times, and their role in intestinal diseases has been studied.


METHODS
In this study colonic epithelial cells isolated from the rat colon were exposed in vitro to various oxidants such as menadione, xanthine-xanthine oxidase, hydrogen peroxide, cumene hydroperoxide, and tertiary butyl hydroperoxide, separately. Changes in cell viability, thiol status, and the antioxidant enzyme activities were measured.


RESULTS
Colonocytes were found to be sensitive to menadione and were not affected by various other oxidants. Decrease in cell viability, depletion of reduced glutathione and protein thiol, and change in antioxidant enzyme activities were observed when the cells were exposed to menadione.


CONCLUSIONS
This study suggests that, unlike other cells, colonocytes are susceptible only to certain selective oxidants.
Baskar
L
L
Welcome Trust Research Laboratory, Dept. of Gastrointestinal Sciences, Christian Medical College Hospital, Vellore, India.
Balasubramanian
K A
KA
D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000201:enzymology; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology*; D010545:Peroxides / Q000494:pharmacology; D051381:Rats; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014812:Vitamin K / Q000494:pharmacology; D019820:Xanthine; D014969:Xanthine Oxidase / Q000494:pharmacology; D014970:Xanthines / Q000494:pharmacology; D020122:tert-Butylhydroperoxide
D001555:Benzene Derivatives; D016877:Oxidants; D010545:Peroxides; D013438:Sulfhydryl Compounds; D014970:Xanthines; D014812:Vitamin K; D019820:Xanthine; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D014969:Xanthine Oxidase; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005978:Glutathione; C007164:cumene hydroperoxide