title: Extent of sequence variability in a genomic region coding for capsid proteins of B19 parvovirus.
abstract: Genomic variability in human parvovirus B19 was analysed by direct partial sequencing of different isolates collected in Italy between 1989 and 1994. DNA was purified from viremic serum samples and the region of viral genome coding for structural proteins was amplified by polymerase chain reaction and partially sequenced (nt. 2400-3400). Data were compared to reference isolates Wi (U.K., 1973) and Au (U.S.A., 1982). The average relative distance between isolates was estimated at the low level of 0.61%, comparable with the distance to reference isolates. Out of 22 nucleotide substitutions found, 9 resulted in amino acid changes. From our results, this region of human parvovirus B19 genome appears to be stably conserved.
authors.affiliation: Institute of Microbiology, University of Bologna, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Gallinella
authors.forename: S
authors.initials: S
authors.lastname: Venturoli
authors.forename: G
authors.initials: G
authors.lastname: Gentilomi
authors.forename: M
authors.initials: M
authors.lastname: Musiani
authors.forename: M
authors.initials: M
authors.lastname: Zerbini
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D017931:DNA Primers; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016732:Parvovirus B19, Human / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers