Evaluation of a new agar in Uricult-Trio for rapid detection of Escherichia coli in urine.
A new commercial agar (Uricult-Trio) with 8-hydroxyquinoline-beta-glucuronide was used to assess 2,536 uropathogens for beta-glucuronidase activity typical of Escherichia coli. Included in the study were 1,807 strains of the family Enterobacteriaceae, 284 strains of nonfermentative bacilli, 345 strains of gram-positive cocci, and 100 yeast strains. In identifying E. coli, the test agar gave a sensitivity of 95.5% and a specificity of 97.2%. Fifty E. coli isolates gave negative reactions; 31 non-E. coli strains produced black colonies characteristic of E. coli. No growth of gram-positive cocci and no false-positive reactions from yeasts were observed. The recovery rate for E. coli on this agar was at least 10% higher than that on blood agar.
Dalet
F
F
Microbiology Department, Puigvert Foundation, Barcelona, Spain.
Segovia
T
T
D000362:Agar; D001431:Bacteriological Techniques / Q000706:statistics & numerical data; D001437:Bacteriuria / Q000175:diagnosis; D003951:Diagnostic Errors; D004926:Escherichia coli / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis; D005069:Evaluation Studies as Topic; D006801:Humans; D006912:Hydroxyquinolines; D011804:Quinolines; D012680:Sensitivity and Specificity; D014552:Urinary Tract Infections / Q000175:diagnosis; D014556:Urine / Q000382:microbiology*
D006912:Hydroxyquinolines; D011804:Quinolines; C056147:8-hydroxyquinoline glucuronide; D000362:Agar