title: Bactericidal effect of bovine normal and immune serum, colostrum and milk against Helicobacter pylori.
abstract: Serum and colostrum but not post-colostral milk from non-immunized Friesian cows was found highly bactericidal for Helicobacter pylori NCTC 11637. This bactericidal activity was destroyed by heating at 56 degrees C for 30 min and restored by the addition of fetal calf serum as a source of complement, indicating that the bacterial effect was probably dependent on an antibody-complement system. Systemic, serial immunization of non-lactating, pregnant cows with H. pylori resulted in high specific antibody titres in serum and colostrum. No titres were found in post-colostral milk, even after booster-immunization during lactation. Immunization did not enhance the bactericidal activity of serum and colostrum, but increased it in post-colostral milk. The bactericidal activity was not correlated with titres of specific antibody or with IgG concentrations.
authors.affiliation: Valio Research & Development Centre, Helsinki, Finland.
authors.forename: H
authors.initials: H
authors.lastname: Korhonen
authors.forename: E L
authors.initials: EL
authors.lastname: Syväoja
authors.forename: H
authors.initials: H
authors.lastname: Ahola-Luttila
authors.forename: S
authors.initials: S
authors.lastname: Sivelä
authors.forename: S
authors.initials: S
authors.lastname: Kopola
authors.forename: J
authors.initials: J
authors.lastname: Husu
authors.forename: T U
authors.initials: TU
authors.lastname: Kosunen
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D001770:Blood Bactericidal Activity; D002417:Cattle; D003126:Colostrum / Q000276:immunology*; D005260:Female; D006361:Heating; D016480:Helicobacter pylori / Q000276:immunology*; D007109:Immunity; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D008892:Milk / Q000276:immunology*; D011247:Pregnancy; D014611:Vaccination / Q000662:veterinary
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D007074:Immunoglobulin G