Molecular cloning and functional characterization of a novel member of the C-C chemokine family.
Chemokines play an important role in immune and inflammatory responses by inducing migration and adhesion of leukocytes. We have isolated a novel chemokine cDNA, designated CCF18, from a cDNA library of an IL-3-dependent murine pro-B cell line, Ba/F3. The cDNA encodes a protein structurally related to the C-C chemokine members. Among this family, C10 shows the highest homology to CCF18, and MIP-1 alpha also has a significant homology but to a lesser extent. CCF18 produced from COS cells induced chemotaxis and Ca2+ flux in CD4+ T cell clones. Moreover, prior administration of MIP-1 alpha desensitized the cells to CCF18. The CCF18 gene (Scya10) was mapped to a middle region of murine chromosome 11, where other genes for several C-C chemokine members are localized. These results clearly indicate that CCF18 is a new member of the C-C chemokine family. Since a high level of CCF18 mRNA is constitutively expressed in macrophage and myeloid cell lines, CCF18 may play a role in inflammatory processes.
Hara
T
T
DNAX Research Institute of Molecular and Cellular Biology, Palo Alto 94304, USA.
Bacon
K B
KB
Cho
L C
LC
Yoshimura
A
A
Morikawa
Y
Y
Copeland
N G
NG
Gilbert
D J
DJ
Jenkins
N A
NA
Schall
T J
TJ
Miyajima
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D018925:Chemokines / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D019742:Chemokines, CC; D002634:Chemotaxis, Leukocyte / Q000502:physiology; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D005260:Female; D015723:Gene Library; D019402:Macrophage Inflammatory Proteins; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D014162:Transfection
C095218:Ccl9 protein, mouse; D018925:Chemokines; D019742:Chemokines, CC; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger