title: Sulfate and thiosulfate transport in Escherichia coli K-12: evidence for a functional overlapping of sulfate- and thiosulfate-binding proteins.
abstract: In Escherichia coli, sulfate and thiosulfate ions are transported by an ABC-type transporter consisting of both the membrane components (the products of cysT, cysW, and cysA genes) and the periplasmic binders (the products of cysP and sbp genes). The single cysP and sbp mutants are able to utilize both sulfate and thiosulfate as a sole sulfur source, while the inactivation of both genes leads to cysteine auxotrophy resulting from the block in the transport of both ions.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.
authors.forename: A
authors.initials: A
authors.lastname: Sirko
authors.forename: M
authors.initials: M
authors.lastname: Zatyka
authors.forename: E
authors.initials: E
authors.lastname: Sadowy
authors.forename: D
authors.initials: D
authors.lastname: Hulanicka
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000235:genetics*; D002455:Cell Division; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D033902:Periplasmic Binding Proteins; D017422:Sequence Analysis, DNA; D013431:Sulfates / Q000378:metabolism*; D013885:Thiosulfates / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; C094586:CysP protein, E coli; D029968:Escherichia coli Proteins; D033902:Periplasmic Binding Proteins; D013431:Sulfates; D013885:Thiosulfates; C489040:sbp protein, E coli; C044773:sulfate-binding protein, bacteria