title: Simple interface for microbore LC and electrospray ionization mass spectrometry and analysis of melphalan-alkylation sites in metallothionein.
abstract: A microbore high pressure liquid chromatograph has been interfaced to a Vestec electrospray ionization source retrofitted to a Hewlett-Packard quadrupole mass spectrometer. The chief features include the absence of a splitter, the use of a second, ballast column to provide a stable flow rate across the gradient, and an in-line UV detector. The system was evaluated for analysis of peptide mixtures and applied to identification of drug-modified peptides released by tryptic digestion of drug-alkylated metallothionein.
authors.affiliation: University of Maryland Baltimore County, Department of Chemistry and Biochemistry 21228, USA.
authors.forename: I A
authors.initials: IA
authors.lastname: Kaltashov
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.forename: C
authors.initials: C
authors.lastname: Fenselau
mesh_terms: D000477:Alkylating Agents; D000478:Alkylation; D000818:Animals; D002853:Chromatography, Liquid; D006868:Hydrolysis; D013058:Mass Spectrometry; D008558:Melphalan; D008668:Metallothionein / Q000032:analysis*; D008970:Molecular Weight; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis; D011817:Rabbits; D013056:Spectrophotometry, Ultraviolet; D014357:Trypsin
chemical_list: D000477:Alkylating Agents; D010455:Peptides; D008668:Metallothionein; D014357:Trypsin; D008558:Melphalan