Separation of peptide derivatives by pH-zone-refining counter-current chromatography.
pH-Zone-refining counter-current chromatography was applied to the preparative separation of oligopeptide derivatives containing up to three amino acids. Both acidic benzyloxycarbonyl peptides and basic peptides-beta-naphthylamide were successfully separated with two-phase solvent systems composed of methyl tert.-butyl ether, 1-butanol, acetonitrile and water using a set of suitable retainer and eluent reagents for each group. The preparative ability of the method was demonstrated in the separation of multigram quantities of analyte with a standard separation column with a total capacity of 325 ml. The effects of important factors such as polarity of the two-phase solvent system, concentrations of the eluent base and the retainer acid were discussed.
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Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
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D000595:Amino Acid Sequence; D002845:Chromatography / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000032:analysis* / Q000737:chemistry
D009842:Oligopeptides