title: A nonmammalian homolog of the PAF1 gene (Zellweger syndrome) discovered as a gene involved in caryogamy in the fungus Podospora anserina.
abstract: The car1 gene of the filamentous fungus Podospora anserina was cloned by complementation of a mutant defective for caryogamy (nuclear fusion), a process required for sexual sporulation. This gene encodes a protein that shows similarity to the mammalian PAF1 protein (Zellweger syndrome). Besides sequence similarity, the two proteins share a transmembrane domain and the same type of zinc finger motif. A combination of molecular, physiological, genetical, and ultrastructural approaches gave evidence that the P. anserina car1 protein is actually a peroxisomal protein. This study shows that peroxisomes are required at a specific stage of sexual development, at least in P. anserina, and that a functional homolog of the PAF1 gene is present in a lower eucaryote.
authors.affiliation: Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France.
authors.forename: V
authors.initials: V
authors.lastname: Berteaux-Lecellier
authors.forename: M
authors.initials: M
authors.lastname: Picard
authors.forename: C
authors.initials: C
authors.lastname: Thompson-Coffe
authors.forename: D
authors.initials: D
authors.lastname: Zickler
authors.forename: A
authors.initials: A
authors.lastname: Panvier-Adoutte
authors.forename: J M
authors.initials: JM
authors.lastname: Simonet
mesh_terms: D000595:Amino Acid Sequence; D001119:Arginase / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D003360:Cosmids; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D005800:Genes, Fungal; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D008830:Microbodies / Q000378:metabolism / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D000074428:Peroxisomal Biogenesis Factor 2; D010957:Plasmids; D011994:Recombinant Proteins / Q000096:biosynthesis; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D013098:Xylariales / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D015211:Zellweger Syndrome / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; D000074428:Peroxisomal Biogenesis Factor 2; D001119:Arginase