Disappearance of the Vicia villosa-positivity from the perineuronal net containing chondroitin proteoglycan after chondroitinase digestion.
Anti-chondroitin unsulfated proteoglycan (COS-PG) 1B5 monoclonal antibody (MAb) and Vicia villosa agglutinin (VVA) recognize the same neuronal subset. Moreover, when in double immunofluorescence study the sections were digested with chondroitinase ABC (ChABC), a procedure necessary to create the epitope of 1B5 MAb, and incubated with VVA, no VVA positive neurons were detected. This finding suggests that VVA binds terminal N-acetyl-galactosamine present in the glycidic chains of COS-PG or of glycoproteins that form perineuronal molecular aggregates with COS-PG.
Bertolotto
A
A
University of Turin, San Luigi Gonzaga Hospital, Centro Sclerosi Multipla, Orbassano, Italy.
Manzardo
E
E
Iudicello
M
M
Lovisetto
C
C
Riccio
A
A
D000818:Animals; D000906:Antibodies / Q000276:immunology; D002807:Chondroitin / Q000378:metabolism*; D002810:Chondroitinases and Chondroitin Lyases / Q000378:metabolism*; D007150:Immunohistochemistry; D009474:Neurons; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000906:Antibodies; D011509:Proteoglycans; D002807:Chondroitin; D002810:Chondroitinases and Chondroitin Lyases