Insulin and insulin-like growth factor I stimulate expression of the primary response gene cMG1/TIS11b by a wortmannin-sensitive pathway in RIE-1 cells.
The addition of insulin or insulin-like growth factor I (IGF-I) to RIE-1 cells increased the expression of the primary response gene cMG1; dose-response analysis suggested that this effect was mediated largely through type 1 IGF receptors. Insulin/IGF-I did not affect the expression of the cMG1-related genes TIS11 and TIS11d, whereas epidermal growth factor, angiotensin II or 12-O-tetradecanoyl phorbol-13-acetate stimulated the expression of all three genes. Incubation with wortmannin (WM) prevented the insulin/IGF-I-induced elevation of cMG1 mRNA, but not that induced by the other mitogens or the stimulation of mitogen-activated protein kinase by insulin. We conclude that WM-sensitive phosphatidylinositol 3-kinase may be involved in the specific stimulation of cMG1 expression by insulin/IGF-I.
Corps
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AN
Department of Cellular Physiology, AFRC Babraham Institute, Cambridge, UK.
Brown
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D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D051817:Butyrate Response Factor 1; D002460:Cell Line; D004268:DNA-Binding Proteins; D005786:Gene Expression Regulation / Q000187:drug effects; D017874:Immediate-Early Proteins; D007328:Insulin / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D011506:Proteins / Q000235:genetics*; D051381:Rats; D051816:Tristetraprolin; D000077191:Wortmannin
D000730:Androstadienes; D051817:Butyrate Response Factor 1; D004268:DNA-Binding Proteins; D017874:Immediate-Early Proteins; D007328:Insulin; D011506:Proteins; D051816:Tristetraprolin; C497554:Zfp36 protein, rat; C497555:Zfp36l1 protein, rat; D007334:Insulin-Like Growth Factor I; D019869:Phosphatidylinositol 3-Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); D000077191:Wortmannin