[Identification of tuberculosis pathogens in clinical material using the polymerase chain reaction].
The authors examined the possibility of detecting M. tuberculosis cells in various types of diagnostic material (sputum, blood, bone marrow, bronchoalveolar lavage fluid) from tuberculosis patients using polymerase chain reaction (PCR). The developed PCR-based test systems helped detect M. tuberculosis in 48 (90.6%) out of 53 tuberculosis patients, in contrast to much slower microbiological methods which permitted detection of Mycobacteria in only 21 (39.6%) patients. High specificity and virtually no false-positive results of PCR were demonstrated in testing diagnostic material from patients with chronic nonspecific pulmonary diseases and from children with lympholeukemia and anemia.
Grishina
T D
TD
Shaginian
I A
IA
Puzanov
V A
VA
Nesterenko
L N
LN
Golyshevskaia
V I
VI
Gintsburg
A L
AL
Khomenko
A G
AG
Prozorovskiĭ
S V
SV
D016470:Bacteremia; D001853:Bone Marrow / Q000382:microbiology; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D014376:Tuberculosis / Q000097:blood / Q000382:microbiology*