title: Regulation of Pax-3 expression in the dermomyotome and its role in muscle development.
abstract: The segmented mesoderm in vertebrates gives rise to a variety of cell types in the embryo including the axial skeleton and muscle. A number of transcription factors containing a paired domain (Pax proteins) are expressed in the segmented mesoderm during embryogenesis. These include Pax-3 and a closely related gene, Pax-7, both of which are expressed in the segmental plate and in the dermomyotome. In this paper, we show that signals from the notochord pattern the expression of Pax-3, Pax-7 and Pax-9 in somites and the subsequent differentiation of cell types that arise from the somitic mesoderm. We directly assess the role of the Pax-3 gene in the differentiation of cell types derived from the dermomyotome by analyzing the development of muscle in splotch mouse embryos which lack a functional Pax-3 gene. A population of Pax-3-expressing cells derived from the dermomyotome that normally migrate into the limb are absent in homozygous splotch embryos and, as a result, limb muscles are lost. No abnormalities were detected in the trunk musculature of splotch embryos indicating that Pax-3 is necessary for the development of the limb but not trunk muscle.
authors.affiliation: Molecular Neurobiology Laboratory, Salk Institute, La Jolla, CA 92037, USA.
authors.forename: M
authors.initials: M
authors.lastname: Goulding
authors.forename: A
authors.initials: A
authors.lastname: Lumsden
authors.forename: A J
authors.initials: AJ
authors.lastname: Paquette
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002490:Central Nervous System; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D008648:Mesoderm / Q000166:cytology / Q000502:physiology*; D051379:Mice; D018482:Muscle, Skeletal / Q000196:embryology*; D009672:Notochord / Q000502:physiology / Q000637:transplantation; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D014157:Transcription Factors
chemical_list: D004268:DNA-Binding Proteins; D000072041:PAX3 Transcription Factor; D051761:Paired Box Transcription Factors; D014157:Transcription Factors; C068686:Pax3 protein, mouse