A simple technique for the long-term non-polarised and polarised culture of human fallopian tube epithelial cells.
Fallopian tubes were obtained from 25 women undergoing abdominal hysterectomy. Pieces of fallopian tube mucosa were placed in culture flasks containing minimum essential medium in Earle's salts supplemented with fetal bovine serum. First passage was carried out after 7-10 days and subcultures in 4-5 days. For polarised cell culture, epithelial cells were seeded onto an extracellular matrix system. New epithelial cells were seen on day 2-3 of the primary culture and epithelial patches on day 7-10. Cells reached confluence in 4-5 days in subcultures. The cells could be subcultured for 7-11 passages with a life span of 42-60 days. Epithelial origins of the cells were confirmed by immunofluorescence staining with anti-cytokeratin antibody. Polarised cells showed a columnar pattern, microvilli on their apical surface and basally located nucleus whereas non-polarised cells were flat. It was concluded that the human fallopian tube epithelial cells can be cultured in vitro to create non-polarised and polarised cell layers by using a simple and reproducible technique and this system can be a potential model to study function of the fallopian tube.
Kervancioglu
M E
ME
Academic Unit of Obstetrics & Gynaecology, London Hospital Medical College, Royal London Hospital Trust, Whitechapel, UK.
Saridogan
E
E
Martin
J E
JE
Maguiness
S D
SD
Djahanbakhch
O
O
D000328:Adult; D016764:Cell Polarity / Q000502:physiology*; D002478:Cells, Cultured; D003470:Culture Media; D004847:Epithelial Cells; D005187:Fallopian Tubes / Q000166:cytology*; D005260:Female; D006801:Humans; D008875:Middle Aged; D015203:Reproducibility of Results; D013997:Time Factors
D003470:Culture Media