title: Two different subpopulations of Ig-bearing cells in lymphoid organs of rainbow trout.
abstract: In a previous report we described the existence of two structural and antigenic variants of light chains in serum trout Igs, defined by mAbs 2H9 and 2A1. In this report, we analyse the cell distribution, the "in vitro" kinetics of production, and the ontogenic appearance of these two variants. Both variants were already present at day 8 before hatching and their kinetics of appearance correlated with that of total Ig, showing a peak around hatching. These mAbs stained two distinct lymphoid cell populations in the pronephros, mesonephros, spleen, and peripheral blood. Both mAbs reacted with approximately 1% of thymocytes. The ratio between these two cell populations (2A1+/2H9+) was about 2/1 in the lymphoid organs analysed. No differences were found between the profiles of the "in vitro" production of these variants in pronephric cell cultures stimulated with LPS. In these cultures, the 2A1 and 2H9 Igs together accounted for 10-70% of the total trout Ig produced, suggesting the existence of at least one additional L chain variant.
authors.affiliation: Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Sánchez
authors.forename: A
authors.initials: A
authors.lastname: Alvarez
authors.forename: A
authors.initials: A
authors.lastname: Castillo
authors.forename: A
authors.initials: A
authors.lastname: Zapata
authors.forename: A
authors.initials: A
authors.lastname: Villena
authors.forename: J
authors.initials: J
authors.lastname: Domínguez
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000921:Antibody-Producing Cells / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis*; D008221:Lymphoid Tissue / Q000166:cytology*; D017686:Oncorhynchus mykiss / Q000254:growth & development / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D007147:Immunoglobulin Light Chains