Localisation of androgen receptors in dermal fibroblasts, grown in vitro, from normal subjects and from patients with androgen insensitivity syndrome.
Human genital skin fibroblasts show the androgen receptor (AR) as bright red granules (0.5 micron in diameter) after treatment with one (of 3) primary polyclonal antibody and a secondary antibody linked to alkaline phosphatase. Almost all AR were cytoplasmic in 12 normal strains and in 8 strains from patients with partial (PAIS) and 6 with complete androgen insensitivity syndrome (CAIS). When 25.8 nM 5-alpha-dihydrotestosterone (DHT) or methyltrienolone was added to the medium, some AR were translocated into the nucleus in 13 experiments with 8 strains of normal fibroblasts; of 8 strains of patients with PAIS 3 failed to translocate to a detectable extent in the presence of androgen in the medium and none of the 6 strains from patients with CAIS translocated.
Jacobson
W
W
University Department of Paediatrics, Addenbrooke's Hospital, Cambridge, UK.
Routledge
J
J
Davies
H
H
Saich
T
T
Hughes
I
I
Brinkmann
A
A
Brown
B
B
Clarkson
P
P
D000728:Androgens / Q000378:metabolism* / Q000494:pharmacology / Q000502:physiology; D001360:Avidin; D002478:Cells, Cultured; D003470:Culture Media; D004700:Endocrine System Diseases / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D011944:Receptors, Androgen / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism*
D000728:Androgens; D003470:Culture Media; D011944:Receptors, Androgen; D018160:Receptors, Cytoplasmic and Nuclear; D001360:Avidin