A combined staphylococcal coagglutination assay for rapid identification of rotavirus and adenovirus (COARA).
An improved staphylococcal coagglutination test was developed for rapid detection, in a single assay, of rotavirus and adenovirus in stool samples (COARA). Suspensions of Staphylococcus aureus coated respectively with anti-rotavirus and anti-adenovirus sera were used to identify these viruses in 327 stool samples of children. The samples were also tested by an enzyme immunoassay. The data analysis has demonstrated a high degree of correlation between the two assays.
Peret
T C
TC
Department of Microbiology, University of São Paulo, Brazil.
Durigon
E L
EL
Candeias
J M
JM
Stewien
K E
KE
Candeias
J A
JA
D000256:Adenoviridae / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D005243:Feces / Q000821:virology; D006168:Guinea Pigs; D006801:Humans; D007124:Immunoenzyme Techniques; D007223:Infant; D011817:Rabbits; D051381:Rats; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus
D000914:Antibodies, Viral