title: M.BssHII: a new multispecific C5-DNA-methyltransferase.
abstract: M.BssHII is a new multispecific C5-DNA-methyltransferase recognizing five different targets. As the enzyme has been isolated from a thermophilic Bacillus, the protein should show enhanced intrinsic thermostability and therefore be a promising candidate for crystallizing a multispecific MTase.
authors.affiliation: Max-Planck-Institut für molekulare Genetik, Berlin, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Schumann
authors.forename: J
authors.initials: J
authors.lastname: Willert
authors.forename: C
authors.initials: C
authors.lastname: Wild
authors.forename: J
authors.initials: J
authors.lastname: Walter
authors.forename: T A
authors.initials: TA
authors.lastname: Trautner
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001412:Bacillus subtilis / Q000201:enzymology; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015257:DNA-Cytosine Methylases / Q000302:isolation & purification / Q000378:metabolism*; D004795:Enzyme Stability; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D013379:Substrate Specificity
chemical_list: D004247:DNA; C094508:BSSHII methyltransferase; D015257:DNA-Cytosine Methylases; D004262:DNA Restriction Enzymes