Differentiation of the rat stria vascularis.
The differentiation of the rat stria vascularis (SV) was investigated by light- and electron microscopy and by immunocytochemistry. Loss of the basal lamina at the epithelial-mesenchymal interface of SVs as indicated by immunoreactions of laminin and fibronectin induces the formation of vascular feet by basal infoldings of the marginal cells (MCs), and the development of the strial capillaries (SCs) by mesenchymal cells in a manner of vasculogenesis is progressing at the same time. The production of fibronectin in the rough endoplasmic reticulum of mesenchymal cells and the involvement of this glycoprotein in a mechanical linkage between the vasoformative mesenchymal cells and endothelial ones of the SCs are indicated by immunocytochemistry. The plasma membrane of the marginal cells (MCs) begins to show immunoreactions of Na+.K+ ATPase at postnatal day 5 and is conjugated to each other by tight junctions at postnatal day 14. The apical tubules of the differentiating MCs do not seem to be involved in the endocytotic activity but are involved in the plasma membrane supply for the rapid differentiation.
Sagara
T
T
Department of Otorhinolaryngology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
Furukawa
H
H
Makishima
K
K
Fujimoto
S
S
D000818:Animals; D002454:Cell Differentiation; D002462:Cell Membrane / Q000201:enzymology; D003053:Cochlear Duct / Q000166:cytology / Q000196:embryology / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005353:Fibronectins / Q000378:metabolism; D007150:Immunohistochemistry; D007797:Laminin / Q000378:metabolism; D008648:Mesoderm / Q000166:cytology; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D016253:Microscopy, Immunoelectron; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013316:Stria Vascularis / Q000166:cytology* / Q000648:ultrastructure
D005353:Fibronectins; D007797:Laminin; D000254:Sodium-Potassium-Exchanging ATPase