Alterations of histone phosphorylation in rat spleen cells after treatment with the aromatic amine, 4,4'-methylene-bis(2-chloroaniline).
Alterations of the phosphorylation pattern of histones by the carcinogen, 4,4'-methylene-bis(2-chloroaniline) (MOCA) were investigated using rodent spleen cells. Spleen cells were isolated from Sprague-Dawley rats and treated with either 5, 10, 25, or 50 microM MOCA or acetone vehicle controls for 1, 2, 4, or 8 hours. Cells were incubated with 32P-phosphoric acid, and histones from these cells were fractionated utilizing two-dimensional polyacrylamide gel electrophoresis. Marked stimulation of histone phosphorylation was observed with the 10 microM MOCA treatment. A transient decrease in histone phosphorylation was observed at the 1 and 2 hour time points followed by a marked stimulation at 4 hours.
DeBord
D G
DG
Department of Health and Human Services, Centers for Disease Control and Prevention, Cincinnati, OH 45226, USA.
Cheever
K L
KL
Booth-Jones
A D
AD
Swearengin
T F
TF
Savage
R E
RE
D000818:Animals; D015415:Biomarkers; D004305:Dose-Response Relationship, Drug; D006657:Histones / Q000378:metabolism*; D066298:In Vitro Techniques; D008753:Methylenebis(chloroaniline) / Q000633:toxicity*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
D015415:Biomarkers; D006657:Histones; D008753:Methylenebis(chloroaniline); D010770:Phosphotransferases