Biochemical characterization of VspI methyltransferase.
The gene (vspIM) encoding VspI methyltransferase (MTase) has previously been cloned and sequenced, and shown to belong to the gamma class of m6-adenine MTases [Degtyarev et al., Nucleic Acids Res. 21 (1993) 2015]. Here it is shown that the MTase modifies the third adenine within the recognition sequence 5'-ATTAAT-3'.
Degtyarev
S Kh
SKh
SibEnzyme, Novosibirsk, Russia.
Prikhod'ko
E A
EA
Rechkunova
N I
NI
Prikhod'ko
G G
GG
Krasnykh
V N
VN
D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D005798:Genes, Bacterial; D007700:Kinetics; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D013379:Substrate Specificity
D011994:Recombinant Proteins; C081230:VspI methylase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)