Lack of protective effect of R(-)-deprenyl on programmed cell death of mouse thymocytes induced by dexamethasone.
R(-)-Deprenyl, an archetypical MAO-B inhibitor, has been shown to delay the onset of the disabling syndrome of Parkinson's disease and to be useful in the treatment of Alzheimer's disease. Recently, R(-)-deprenyl has been claimed to be capable of preventing apoptosis of PC12 cells, which had been primed with nerve growth factor (NGF) and followed by withdrawal of serum. We investigated the effect of R(-)-deprenyl in a non-neuronal cell model, namely, apoptosis of mouse thymocytes induced by dexamethasone. Trypan blue exclusion and lactate dehydrogenase activity were applied to assess the cell survival. R(-)-Deprenyl did not exhibit any detectable protective effect to the thymocytes from apoptosis. The result is further confirmed by examining the apoptotic DNA fragmentation using gel electrophoresis and assessing the soluble DNA released by a spectrophotometric method.
Fang
J
J
Department of Psychiatry University of Saskatchewan, Saskatoon, Canada.
Zuo
D M
DM
Yu
P H
PH
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D003907:Dexamethasone / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D051379:Mice; D012642:Selegiline / Q000494:pharmacology*; D013237:Stereoisomerism; D013950:Thymus Gland / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D014343:Trypan Blue / Q000493:pharmacokinetics
D012642:Selegiline; D003907:Dexamethasone; D004247:DNA; D007770:L-Lactate Dehydrogenase; D014343:Trypan Blue