Dog neutrophil stimulation and cell surface expression of heat shock proteins induced by AHN 086.
The effect of AHN 086 [1-(2-isothiocyanatoethyl)-7-chloro-1,3-dihydro-5-(4- chloro-phenyl)-2H-1,4-benzodiazepine-2-one hydrochloride], an irreversible ligand of the mitochondrial benzodiazepine receptor, was studied in the heat shock protein (HSP) expression and the activation of dog neutrophils. At micromolar concentrations, AHN 086 induced a dose-dependent increase in free radical production (309 +/- 13% at 1 x 10(-5) M). This compound also increased the phagocytic activity (73 +/- 3.5% at 5 x 10(-6) M). Immunohistochemical results showed that AHN 086 induced the expression of HSP in neutrophil membranes. At 1 x 10(-5) M, the percentage of positive cells was: 90.2 (27 kD), 90.6 (72 kD) and 92.8 (90 kD). This expression was accompanied by a decrease in the presence of nuclear and cytoplasmic HSP. Flow cytometry studies showed that this cell membrane expression was located on the neutrophil surface and could be a consequence of HSP mobilization from nucleus and cytoplasm.
Camins
A
A
Laboratory of Pharmacology and Pharmacognosy, Faculty Pharmacy, University of Barcelona, Spain.
Escubedo
E
E
Camarasa
J
J
Carretero
J
J
Newman
A H
AH
Kammüller
M E
ME
D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004285:Dogs; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D007150:Immunohistochemistry; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress; D010587:Phagocytosis / Q000187:drug effects; D016897:Respiratory Burst / Q000187:drug effects
D001570:Benzodiazepinones; D006360:Heat-Shock Proteins; C049442:AHN 086