DNA damage and cancer: measurement and mechanism.
There is currently great interest in the possible role of reactive nitrogen species and reactive oxygen species in causing DNA damage that leads to cancer. It appears likely that certain reactive oxygen species can act as complete carcinogens. However, the development of human cancer will depend on other factors such as the extent of DNA damage, antioxidant levels and DNA repair systems. The true picture will only be seen if we have reliable and sensitive techniques for the measurement of DNA damage. In this article we outline various methods for measuring DNA damage base, with special emphasis on HPLC and GC-MS based systems.
Wiseman
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Department of Nutrition and Dietetics, King's College London, Kensington, UK.
Kaur
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Halliwell
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B
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004249:DNA Damage; D004260:DNA Repair; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006147:Guanine / Q000031:analogs & derivatives / Q000652:urine; D006801:Humans; D017672:Nitrogen Compounds / Q000032:analysis / Q000633:toxicity*; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000032:analysis / Q000633:toxicity*
D017672:Nitrogen Compounds; D017382:Reactive Oxygen Species; C024829:8-hydroxyguanine; D006147:Guanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine