[Effects of the maturity of human oocytes on fertilization and embryonic development: application of oocyte maturation in medium containing follicle-stimulating hormone].
A total of 2,145 oocytes from 355 IVF cycles were classified as overmature (O), mature (M), transitional (T), immature (I) and abnormal (A) according to the morphology of the corona radiata. The rates of fertilization (%F) and embryonic development (%D) per oocyte were the highest in the M group, and decreased significantly with the decreasing maturity of the oocytes (T and I) and were the lowest in the A group. Decreases in %F and %D were also observed in the O group. %F and %D were significantly higher in oocytes with a polar body (PB) than without a PB. Mature oocytes classified according to the morphology of the corona radiata had significantly higher %F and %D than those classified according to the morphology of the cumulus oophorus. %F and %D were increased when T-oocytes without a PB and I-oocytes were matured in vitro for 18-24 hours in medium supplemented with FSH. A normal female baby was delivered, following IVF-ET of two T-oocytes matured in vitro with FSH.
Jinno
M
M
Department of Obstetrics and Gynecology, School of Medicine, Kyorin University, Tokyo.
Hanyu
I
I
Ubukata
Y
Y
Satou
M
M
Katsumata
Y
Y
Yoshimura
Y
Y
Nakamura
Y
Y
D000328:Adult; D005314:Embryonic and Fetal Development / Q000187:drug effects*; D005260:Female; D005306:Fertilization / Q000187:drug effects*; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006801:Humans; D007231:Infant, Newborn; D009865:Oocytes / Q000254:growth & development*; D009866:Oogenesis / Q000187:drug effects*
D005640:Follicle Stimulating Hormone