Bradykinin induces rise of free calcium in nuclei of neuroblastoma x glioma hybrid NG 108-15 cells.
Confocal fluorescence microscopy was used to study the bradykinin-induced calcium signals in the neuroblastoma x glioma cell line NG 108-15. We found that bradykinin induced a rise in free calcium, not only in the cytoplasm but also in the nucleus. The nuclear and cytosolic calcium concentrations were not significantly different and rose to about 1.2 microM. The signal was mediated by the B2-receptor subtype as confirmed using the specific antagonist Hoe 140. Both the onset and the intensity of the calcium signals were concentration-dependent. The rise of nuclear calcium level was independent of extracellular calcium and suppressed by thapsigargin which is known to deplete inositol 1,4,5-trisphosphate-sensitive calcium stores. Bradykinin-induced calcium increase desensitizes rapidly. This desensitization was shown not to involve activation of protein kinase C.
Beckmann
R
R
Institute for Biochemistry, Free University Berlin, Germany.
Lindschau
C
C
Haller
H
H
Buchner
K
K
D000818:Animals; D001920:Bradykinin / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D016257:Fura-2; D005910:Glioma / Q000378:metabolism* / Q000648:ultrastructure; D006822:Hybrid Cells; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D018613:Microscopy, Confocal; D009447:Neuroblastoma / Q000378:metabolism* / Q000648:ultrastructure; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D014407:Tumor Cells, Cultured
C065679:icatibant; D015544:Inositol 1,4,5-Trisphosphate; D011493:Protein Kinase C; D001920:Bradykinin; D002118:Calcium; D016257:Fura-2