title: Effects of prenatal exposure to low dose ionizing radiation on the development of the cerebellar cortex in the rat.
abstract: The effects of maternal exposure to a single dose of whole body irradiation (0.5 Gy) on gestational days (GD) 17, 18, 19, or 20 on the development of the cerebellar cortex was examined in the offspring of Sprague Dawley rats at 21 and 28 days postnatally. No gross cerebellar anomalies were observed in the irradiated animals. However, compared to control animals, rat irradiated on each of GD-17, 18, 19 and 20 showed a significantly higher incidence (p < 0.05) of circumscribed cerebellar lesions (CL) distributed in the inner granular layer of the anterior and posterior lobes. These lesions were characterized by a loss of granule cells and atrophied and/or reduced number of Purkinje cells. In 21 days old rats, irradiation on GD-17 resulted in more CL anteriorly (75%) and in the vermis whereas on GD-20, the CL predominated posteriorly (100%) and in the lateral hemispheres. In 28 day old rats, following irradiation on each of GD-17 and GD-20, there was an equal distribution of CL in both the anterior and posterior lobes. However, with irradiation on both GD-17 and GD-20, these CL occurred more frequently in the lateral hemispheres of the anterior lobe, whereas in the posterior lobe they predominated in the vermis. These results suggest that a direct relationship exists between the proliferation, migration, development, and maturation of granule cells and their induction by Purkinje cells. The findings also support the view that both cell death and the regulation of granule cells by Purkinje cells maximize the effective development and organization of the cerebellum.
authors.affiliation: Department of Anatomy, University of Manitoba, Winnipeg, Canada.
authors.forename: T A
authors.initials: TA
authors.lastname: Ralcewicz
authors.forename: T V
authors.initials: TV
authors.lastname: Persaud
mesh_terms: D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D002465:Cell Movement / Q000528:radiation effects; D002525:Cerebellar Cortex / Q000166:cytology / Q000254:growth & development* / Q000528:radiation effects*; D005260:Female; D005865:Gestational Age; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D011689:Purkinje Cells / Q000528:radiation effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D014916:Whole-Body Irradiation