Keyhole limpet hemocyanin contains Gal(beta 1-3)-GalNAc determinants that are cross-reactive with the T antigen.
Keyhole limpet hemocyanin (KLH) is widely used as a carrier molecule to enhance immune responses to administered antigens, and for immunotherapy of bladder and renal carcinoma. In the present study we show, using lectin and antibody binding studies, that native KLH contains Gal(beta 1-3)GalNAc-bearing oligosaccharides, and that immunization with KLH in Lewis rats induces the production of anti-Gal(beta 1-3) GalNAc antibodies. This might explain the beneficial effect of KLH in bladder cancers that express cross-reactive Gal(beta 1-3)GalNAc determinants or the T antigen.
Wirguin
I
I
Department of Neurology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA.
Suturkova-Milosević
L
L
Briani
C
C
Latov
N
N
D000818:Animals; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000276:immunology*; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D005260:Female; D005677:G(M1) Ganglioside / Q000276:immunology; D006031:Glycosylation; D006433:Hemocyanins / Q000737:chemistry / Q000276:immunology*; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D037102:Lectins / Q000378:metabolism; D008969:Molecular Sequence Data; D019887:Peanut Agglutinin; D051381:Rats; D011917:Rats, Inbred Lew
D015295:Antigens, Tumor-Associated, Carbohydrate; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D037102:Lectins; D019887:Peanut Agglutinin; C016467:Thomsen-Friedenreich antigen; D005677:G(M1) Ganglioside; C018835:asialo GM1 ganglioside; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin