An improved SDS-polyacrylamide gel electrophoresis for resolution of peptides in the range of 3.5-200kDa.
Resolution of a wide range of polypeptides, 3.5-200kDa, on a single low acrylamide and cross linkage gel of 7.7% T, 2.6% C is described here. Laemmli's (4) original discontinuous SDS polyacrylamide gel electrophoresis (SDS-PAGE) system is modified by increasing the ionic strength of both stacking and resolving gels, and replacing the usual glycine buffer with a tricine cathode buffer as described by Schagger and von Jagow (7). This system offers the advantage of a wide range of protein fractionation, with sufficient band resolution, on a single, low acrylamide concentration and cross linkage gel. Moreover, increased gel ionic concentration allows higher protein and salt load, and renders this system suitable for preparative work.
Khalkhali-Ellis
Z
Z
Department of Biology, Washington University, St. Louis, MO 63130, USA.
D000818:Animals; D002021:Buffers; D002645:Chickens; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005782:Gels; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009994:Osmolar Concentration; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
D002021:Buffers; D005782:Gels; D011506:Proteins