title: Confirmation of the identity of human skeletal remains using Multiplex PCR Amplification and Typing Kits.
abstract: The identify of human skeletal remains found in a wooded area approximately one year after the person was reported missing was provisionally established by routine methods and circumstantial evidence. Multiplex PCR systems--the AmpliType PM PCR Amplification and Typing Kit and the GenePrint STR Triplex Amplification and Typing Kit--were used to confirm the identification. DNA profiles from femur bone from the remains were compared with profiles derived from head hairs from a hairbrush recovered in the missing woman's apartment. In addition, a sex typing procedure using the X-Y homologous gene amelogenin was carried out. This is the first report of a case using commercially available multiplex PCR amplification and typing kits to confirm the identity of skeletal remains.
authors.affiliation: DNA Identity Laboratory, University of Berne, Switzerland.
authors.forename: M N
authors.initials: MN
authors.lastname: Hochmeister
authors.forename: B
authors.initials: B
authors.lastname: Budowle
authors.forename: U V
authors.initials: UV
authors.lastname: Borer
authors.forename: O
authors.initials: O
authors.lastname: Rudin
authors.forename: M
authors.initials: M
authors.lastname: Bohnert
authors.forename: R
authors.initials: R
authors.lastname: Dirnhofer
mesh_terms: D001842:Bone and Bones; D004247:DNA / Q000032:analysis*; D018732:Forensic Anthropology; D006197:Hair; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D004247:DNA