Detection and counting of specific cell populations by means of magnetic markers linked to monoclonal antibodies.
We report on experiments aimed at the assessment of a new method for cell marking. This method combines superparamagnetic particles, commonly used for cell separation, linked to monoclonal antibodies, and biomagnetic instrumentation featuring an extremely high magnetic field sensitivity. The final goal of the method is to locate and estimate specific cell populations in the human body. In this experiment, quantitative features of the method are evaluated in vitro with lymphocytes and carcinoma cells. Comparison between estimation and direct counting of cells is quite satisfactory and motivates further development of the technique.
Del Gratta
C
C
Institute of Medical Physics, University G D Annunzio, Chieti, Italy.
Della Penna
S
S
Battista
P
P
Di Donato
L
L
Vitullo
P
P
Romani
G L
GL
Di Luzio
S
S
D000911:Antibodies, Monoclonal; D002452:Cell Count / Q000379:methods*; D002460:Cell Line; D003110:Colonic Neoplasms / Q000473:pathology*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D008280:Magnetics; D008962:Models, Theoretical; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal