title: Two-dimensional crystallization and preliminary structure analysis of light harvesting II (B800-850) complex from the purple bacterium Rhodovulum sulfidophilum.
abstract: Light-harvesting complex II (B800/850) from the purple bacterium Rhodovulum (Rhv.) sulfidophilum has been isolated using a new protocol. It has been shown by analytical ultracentrifugation and native gels to be most likely an octamer. Two-dimensional crystals have been obtained by microdialysis. The plane group is p4212 with a = b = 157 A. The crystals diffract to 18 A in negative stain. Projection maps show clearly that LHII is organized in ring-like particles with an outer diameter of about 76 A.
authors.affiliation: European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Montoya
authors.forename: M
authors.initials: M
authors.lastname: Cyrklaff
authors.forename: I
authors.initials: I
authors.lastname: Sinning
mesh_terms: D001419:Bacteria / Q000737:chemistry*; D001426:Bacterial Proteins; D003460:Crystallization; D007091:Image Processing, Computer-Assisted; D045342:Light-Harvesting Protein Complexes; D017551:Microdialysis; D008854:Microscopy, Electron; D015394:Molecular Structure; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000648:ultrastructure
chemical_list: D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; C081485:light-harvesting protein complex II, Rhodobacter capsulatus