title: Analysis of T-cell and B-cell responses to recombinant M. leprae antigens in leprosy patients and in healthy contacts: significant T-cell responses to antigens in M. leprae nonresponders.
abstract: The recognition of a panel of recombinant Mycobacterium leprae antigens by T cells and B cells from 29 borderline tuberculoid/tuberculoid (BT/TT) and 18 lepromatous leprosy (LL) patients and from 21 healthy controls (HC) in leprosy-endemic regions of Ethiopia was examined. All 11 antigenic molecules tested (including M. leprae hsp 10, hsp18, hsp65 and several novel M. leprae antigens) were shown to be recognized by T cells, but clear quantitative differences existed between reactivities induced by individual antigens. Similar quantitative differences were observed when antibody responses to hsp10 and hsp65 antigens were determined. No associations were found between the antigen-specific responses and the subject status of either BT/TT and LL patients or HC. Fifteen percent of the patients who were nonresponsive to sonicates of M. leprae showed significant T-cell responses to one or more individual M. leprae antigens. This indicates that M. leprae constituents other than the proteins tested are responsible for the M. leprae-specific nonresponsiveness in these patients, which may be exploited for the design of vaccines or immunotherapeutic modalities aimed at inducing M. leprae-specific immunity in nonresponders.
authors.affiliation: Department of Immunohaematology and Bloodbank, University Hospital Leiden, The Netherlands.
authors.forename: J E
authors.initials: JE
authors.lastname: Thole
authors.forename: A A
authors.initials: AA
authors.lastname: Janson
authors.forename: A
authors.initials: A
authors.lastname: Kifle
authors.forename: R C
authors.initials: RC
authors.lastname: Howe
authors.forename: K
authors.initials: K
authors.lastname: McLean
authors.forename: A
authors.initials: A
authors.lastname: Nurilygn
authors.forename: E
authors.initials: E
authors.lastname: Filley
authors.forename: E J
authors.initials: EJ
authors.lastname: Shannon
authors.forename: G J
authors.initials: GJ
authors.lastname: Bulla
authors.forename: J
authors.initials: J
authors.lastname: Hermans
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology; D005002:Ethiopia; D006360:Heat-Shock Proteins / Q000276:immunology; D006801:Humans; D007918:Leprosy / Q000276:immunology*; D015439:Leprosy, Borderline / Q000276:immunology; D015440:Leprosy, Lepromatous / Q000276:immunology; D015441:Leprosy, Tuberculoid / Q000276:immunology; D008213:Lymphocyte Activation; D009166:Mycobacterium leprae / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D011993:Recombinant Fusion Proteins