An ascending non-obstructive model for chronic pyelonephritis in BALB/c mice.
Chronic pyelonephritis was successfully produced in female BALB/c mice with Escherichia coli after introduction of the bacterial inoculum by the ascending route. E. coli strain 31-B, a nalidixic acid-resistant derivative of strain TN675 expressing type-1 pili, and E. coli strain BH-5, a mutant of 31-B, not expressing type-1 pili, were used in the model. Both the strains were able to induce chronic renal inflammation in the experimental animals. Whereas the initial colonisation was greater with strain 31-B, its non-pilate mutant BH-5 caused a significantly greater inflammatory response and also caused renal scarring observable 5 months after the infection.
Gupta
R
R
Department of Microbiology, Government Medical College, Chandigarh, India.
Ganguly
N K
NK
Ahuja
V
V
Joshi
K
K
Sharma
S
S
D000818:Animals; D002908:Chronic Disease; D004195:Disease Models, Animal; D004926:Escherichia coli / Q000502:physiology* / Q000648:ultrastructure; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000473:pathology; D005260:Female; D010861:Fimbriae, Bacterial / Q000502:physiology; D007668:Kidney / Q000382:microbiology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D011704:Pyelonephritis / Q000382:microbiology* / Q000473:pathology