Differential sensitivity to antigenic competition in antigen-specific and -nonspecific antigen presentation by B cells.
We have previously shown that a specific Ag presentation by B cells is different from a nonspecific one in the sensitivity to protein synthesis inhibition. In the present study we have compared the sensitivity of these Ag presentations to antigenic competition. A20-HL cells expressing TNP-specific IgM were pulsed with anti-mouse IgM goat IgG (aMGG) or trinitrophenylated goat IgG (TNP-NGG) as an Ag internalized through Ag receptor or NGG as an Ag internalized by fluid-phase pinocytosis. The pulsed cells induced IL-2 production by NGG-specific cloned T cells. The presence of dog IgG during pulsing A20-HL cells severely inhibited the presentation of NGG but not of aMGG or TNP-NGG. The presence did not decrease the internalization of 125I-NGG into A20-HL cells, suggesting that the inhibition was localized into the complex formation of antigenic peptides with MHC class II molecules. Thus, a specific Ag presentation by A20-HL cells is different from a nonspecific one in its sensitivity to antigenic competition.
Kakiuchi
T
T
Department of Immunology, Toho University School of Medicine, Tokyo, Japan.
Okada
Y
Y
Kokuho
T
T
Gyotoku
Y
Y
Mizuguchi
J
J
Nariuchi
H
H
D000818:Animals; D017951:Antigen Presentation / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D001667:Binding, Competitive / Q000276:immunology; D004285:Dogs; D000939:Epitopes / Q000235:genetics / Q000378:metabolism*; D006041:Goats; D006241:Haptens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology; D014407:Tumor Cells, Cultured
D000939:Epitopes; D006241:Haptens; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G; D014301:Trinitrobenzenes