GTBP, a 160-kilodalton protein essential for mismatch-binding activity in human cells.
DNA mismatch recognition and binding in human cells has been thought to be mediated by the hMSH2 protein. Here it is shown that the mismatch-binding factor consists of two distinct proteins, the 100-kilodalton hMSH2 and a 160-kilodalton polypeptide, GTBP (for G/T binding protein). Sequence analysis identified GTBP as a new member of the MutS homolog family. Both proteins are required for mismatch-specific binding, a result consistent with the finding that tumor-derived cell lines devoid of either protein are also devoid of mismatch-binding activity.
Palombo
F
F
Istituto di Ricerche di Biologia Molecolare P. Angeletti, Pomezia, Italy.
Gallinari
P
P
Iaccarino
I
I
Lettieri
T
T
Hughes
M
M
D'Arrigo
A
A
Truong
O
O
Hsuan
J J
JJ
Jiricny
J
J
D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D003001:Cloning, Molecular; D015179:Colorectal Neoplasms; D004260:DNA Repair / Q000235:genetics; D004273:DNA, Neoplasm / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009692:Nucleic Acid Heteroduplexes / Q000378:metabolism*; D017421:Sequence Analysis; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; C094443:G-T mismatch-binding protein; D009692:Nucleic Acid Heteroduplexes