The properties of scrambled hirudins.
Scrambled hirudins serve as relevant folding intermediates during in vitro renaturation of the reduced/denatured hirudin. Eleven species of scrambled hirudins were isolated and structurally identified. Their properties, including their relative stability and the kinetics of their consolidation (disulfide reshuffling) to attain the native structure, have been investigated and are reported here.
Chang
J Y
JY
Pharmaceuticals Research Laboratories, Ciba-Geigy Ltd., Basel, Switzerland.
D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000737:chemistry; D019791:Guanidine; D006146:Guanidines / Q000494:pharmacology; D006629:Hirudins / Q000737:chemistry*; D010084:Oxidation-Reduction; D010446:Peptide Fragments / Q000302:isolation & purification; D011489:Protein Denaturation; D017510:Protein Folding; D012697:Serine Endopeptidases / Q000378:metabolism; D014508:Urea / Q000494:pharmacology
D004220:Disulfides; D006146:Guanidines; D006629:Hirudins; D010446:Peptide Fragments; D014508:Urea; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase; D019791:Guanidine