title: Hyperglycemia and non-enzymatic glycation of serum and tissue proteins in chickens.
abstract: The objectives of these studies were to determine whether elevated plasma glucose concentrations in broiler breeder chickens (200-250 mg/dl) can result in the non-enzymatic attachment of glucose to serum proteins (fructosamine) and eventual cross-linking of tissue proteins (basement membrane thickness), and to investigate the effects of a factor that may influence this cross-linking process. In response to feeding the satiety factor calcium propionate (CaP, 1.7%), plasma glucose and fructosamine concentrations were increased (P < 0.05) from 1 to 9 weeks of age, whereas concentrations of plasma glucose and fructosamine in feed-restricted chicks were reduced for the first 7 weeks after hatch. In a second study, the age-related increase in kidney capillary basement membrane thickness was prevented (P < 0.05) by feeding the cross-linking inhibitor aminoguanidine (AG, 800 ppm) to 30-week-old broiler breeder hens for 34 weeks. The results from these studies suggest that concentrations of plasma glucose in chickens may, in fact, be exerting long-term detrimental effects on tissue proteins, which can be ameliorated by factors that limit the cross-linking reaction.
authors.affiliation: Department of Animal and Veterinary Sciences, West Virginia University, Morgantown 26506, USA.
authors.forename: H
authors.initials: H
authors.lastname: Klandorf
authors.forename: S B
authors.initials: SB
authors.lastname: Holt
authors.forename: J A
authors.initials: JA
authors.lastname: McGowan
authors.forename: Y
authors.initials: Y
authors.lastname: Pinchasov
authors.forename: D
authors.initials: D
authors.lastname: Deyette
authors.forename: R A
authors.initials: RA
authors.lastname: Peterson
mesh_terms: D000284:Administration, Oral; D000375:Aging / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D001485:Basement Membrane / Q000187:drug effects; D001786:Blood Glucose / Q000378:metabolism*; D002645:Chickens; D003432:Cross-Linking Reagents; D005260:Female; D005508:Food Deprivation / Q000502:physiology; D019270:Fructosamine; D006031:Glycosylation; D006146:Guanidines / Q000008:administration & dosage / Q000633:toxicity*; D006595:Hexosamines / Q000097:blood*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D011422:Propionates / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology; D011897:Random Allocation
chemical_list: D001786:Blood Glucose; D003432:Cross-Linking Reagents; D006146:Guanidines; D006595:Hexosamines; D011422:Propionates; D019270:Fructosamine; C514136:calcium propionate; C029658:propionic acid; C004479:pimagedine