title: Detection of amplified Y chromosome-specific sequence by capillary electrophoresis with laser-induced fluorescence.
abstract: OBJECTIVE
  To develop a sensitive method for genetic diagnosis using capillary electrophoresis with laser-induced fluorescence.
  
  
  METHODS
  Using male DNA diluted with female DNA as an example, ZFY gene from Y chromosome was amplified specifically by polymerase chain reaction (PCR) with fluorescence-labeled primers and detected by capillary electrophoresis with laser-induced fluorescence.
  
  
  METHODS
  Laser operating laboratory.
  
  
  METHODS
  Human male and female DNA were extracted from healthy human male and female subjects.
  
  
  METHODS
  None.
  
  
  METHODS
  The concentration of human DNA was determined by using a spectrophotometer at an absorbance of 260 nm.
  
  
  RESULTS
  Deoxyribonucleic acid fragments amplified from a single copy of ZFY gene were detected by capillary electrophoresis with laser-induced fluorescence after 35 cycles of PCR amplification.
  
  
  CONCLUSIONS
  This method is potentially applicable for rapid and sensitive detection of fetal Y chromosome DNA sequence in maternal circulation and of single-cell DNA diagnosis.
authors.affiliation: Advanced Technology Center, Beckman Instruments Inc., Fullerton, California 92634, USA.
authors.forename: M S
authors.initials: MS
authors.lastname: Liu
authors.forename: S
authors.initials: S
authors.lastname: Rampal
authors.forename: R A
authors.initials: RA
authors.lastname: Evangelista
authors.forename: G C
authors.initials: GC
authors.lastname: Lee
authors.forename: F T
authors.initials: FT
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D004586:Electrophoresis; D005260:Female; D005453:Fluorescence; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D014998:Y Chromosome
chemical_list: D004247:DNA