RAPD analysis of Aeromonas salmonicida and Aeromonas hydrophila.
The randomly amplified polymorphic DNA (RAPD) technique was used to analyse the genetic differentiation of 13 strains of Aeromonas salmonicida subsp. salmonicida, and seven strains of Aer. hydrophila. Reproducible profiles of genomic DNA fingerprints were generated by polymerase chain reaction (PCR) using a single randomly designed primer. The RAPD profiles of all the non-motile aeromonads, Aer. salmonicida subsp. salmonicida were identical. However, profiles of the motile aeromonads, Aer. hydrophila differed between isolates. These findings reveal genomic homogeneity in Aer. salmonicida subsp. salmonicida and genetic variety in Aer. hydrophila strains.
Miyata
M
M
Department of Aquatic Biosciences, Tokyo University of Fisheries, Japan.
Aoki
T
T
Inglis
V
V
Yoshida
T
T
Endo
M
M
D000333:Aeromonas / Q000145:classification* / Q000235:genetics; D016980:Aeromonas hydrophila / Q000145:classification* / Q000235:genetics; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002465:Cell Movement; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D014644:Genetic Variation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
D004269:DNA, Bacterial