title: Production of functional chick liver HMG 2a protein in Escherichia coli.
abstract: An efficient Escherichia coli system for the production of a variant form of high-mobility group-2a protein (HMG 2a), having the additional 5 amino acid residues (Ala-Pro-Thr-Leu-Glu) at the NH2-terminal, has been constructed. cDNA encoding HMG 2a was ligated with the Omp A signal peptide sequence and was inserted into an inducible bacterial expression vector pSH-L. After the plasmid introduced into E. coli was expressed by temperature shift, the recombinant product was purified by trichloacetic acid precipitation followed by Bio-Rex 70 column chromatography. The purified product showed the expected NH2-terminal sequence and the superhelical activity of circular DNA similar to the authentic HMG 2a isolated from chick liver.
authors.affiliation: Department of Nutrition, School of Medicine, University of Tokushima, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Oka
authors.forename: T
authors.initials: T
authors.lastname: Sasakawa
authors.forename: K
authors.initials: K
authors.lastname: Miyamoto
authors.forename: M
authors.initials: M
authors.lastname: Kuwahata
authors.forename: T
authors.initials: T
authors.lastname: Sassa
authors.forename: S
authors.initials: S
authors.lastname: Horiuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Natori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006609:High Mobility Group Proteins / Q000096:biosynthesis* / Q000235:genetics; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D006609:High Mobility Group Proteins; D011994:Recombinant Proteins