title: HIV-1 DNA in fibroblast cultures infected with urine from HIV-seropositive cytomegalovirus (CMV) excretors.
abstract: Interactions between HIV-1 and CMV may be important in the pathogenesis of AIDS. We have studied whether active CMV infection alters the cell tropism of HIV-1 in dually-infected individuals. Urines from HIV-seropositive individuals excreting CMV were compared to urines from CMV non-excretors. Sixty-six urines from HIV-seropositive individuals were tested. Infectious HIV-1 was not detected in any of the concentrated urines tested. The urines were filtered, concentrated, DNase-treated and cultured on HIV-1 non-permissive human forestin fibroblasts. HIV-1 DNA was detected by PCR with pol gene primers in 5 of 39 MRHF cell cultures inoculated with CMV culture positive urine (p = 0.037). HIV-1 DNA was not detected by PCR in uninfected fibroblasts, in fibroblasts inoculated with CMV uninfected urine from 27 HIV-seropositive patients or in fibroblasts cultured with 9 CMV culture positive urines from 16 HIV-seronegative renal transplant recipients. Supernatant fluid from an HIV-1 PCR-positive culture was passaged onto another fibroblast monolayer, and these cells were negative for HIV-1 DNA. Direct inoculation of fibroblasts with HIV-1 did not yield evidence of infection by PCR. CMV infection may facilitate HIV-1 DNA entry into ordinarily non-permissive cells.
authors.affiliation: Infectious Disease Unit, Massachusetts General Hospital, Harvard Medical School, Boston, USA.
authors.forename: M
authors.initials: M
authors.lastname: Margalith
authors.forename: R T
authors.initials: RT
authors.lastname: D'Aquila
authors.forename: D J
authors.initials: DJ
authors.lastname: Manion
authors.forename: N
authors.initials: N
authors.lastname: Basgoz
authors.forename: L J
authors.initials: LJ
authors.lastname: Bechtel
authors.forename: B R
authors.initials: BR
authors.lastname: Smith
authors.forename: J C
authors.initials: JC
authors.lastname: Kaplan
authors.forename: M S
authors.initials: MS
authors.lastname: Hirsch
mesh_terms: D002478:Cells, Cultured; D003586:Cytomegalovirus Infections / Q000652:urine*; D004279:DNA, Viral / Q000032:analysis*; D005347:Fibroblasts / Q000821:virology; D006679:HIV Seropositivity / Q000821:virology*; D015497:HIV-1 / Q000235:genetics*; D006801:Humans
chemical_list: D004279:DNA, Viral