Cloning of two chitin synthase gene fragments from a protoplastic entomophthorale.
Chitin synthase expression was studied in spontaneously produced protoplasts and in hyphal bodies of the Entomophthorale species Entomophaga aulicae. The absence of wall in protoplasts was correlated to an absence of chitin synthase. Two chitin synthase activities with different biochemical characteristics have been detected in the hyphal bodies. Two chitin synthase gene fragments EaCHS1 and EaCHS2 of 600 bp were obtained using PCR amplification of genomic DNA. Their amino acid sequences showed 75% identity. Compared with other fungal chitin synthases, they belong to class II. EaCHS1 and EaCHS2 were used to probe total RNA from E. aulicae hyphal bodies and protoplasts. A single transcript of 2.4 kb hybridized only with EaCHS1 in protoplasts and hyphal bodies.
Thomsen
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L
Unité de Mycologie, Institut Pasteur, Paris, France.
Beauvais
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D002687:Chitin Synthase / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D004773:Entomophthora / Q000201:enzymology* / Q000235:genetics; D008969:Molecular Sequence Data; D011523:Protoplasts / Q000201:enzymology*
D002687:Chitin Synthase