Physical and linkage mapping of the bovine genome with cosmids.
We have initiated a mapping strategy using cosmid clones to chromosomally anchor a high-resolution bovine genetic linkage map. Ten cosmids containing microsatellites were assigned to bovine chromosomes by fluorescence in situ suppression hybridization (FISH). Four cosmid clones, three of which contain an informative microsatellite, were assigned to autosomes 5, 13, 24, and 28. The assignment to autosome 13 anchors bovine syntenic group U11. Two additional cosmid clones, each containing informative microsatellites, are assigned to autosomes 9 and 29, anchoring bovine linkage groups U2 and U8, respectively. Four cosmid clones, three of which contain informative microsatellites, also provide the first assignment to autosome 25, anchoring bovine syntenic group U7 and orienting the corresponding linkage group relative to the centromere.
Hawkins
G A
GA
USDA, Roman L. Hruska U.S. Meat Animal Research Center (MARC), Clay Center, Nebraska 68933, USA.
Toldo
S S
SS
Bishop
M D
MD
Kappes
S M
SM
Fries
R
R
Beattie
C W
CW
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods*; D003360:Cosmids / Q000235:genetics*; D004276:DNA, Satellite / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D016678:Genome; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data
D004276:DNA, Satellite; D005819:Genetic Markers