title: Disruption of the target G-C base-pair by the HhaI methyltransferase.
abstract: HhaI MTase binds DNA duplexes containing mismatches at the target base position with higher affinity than that observed for the canonical substrate. The stability of these MTase-DNA complexes inversely correlates with the strength of the base pair that is disrupted upon interaction. This finding may offer a general tool to detect other enzymes that flip bases out of the DNA helix.
authors.affiliation: Cold Spring Harbor Laboratory, NY 11724, USA.
authors.forename: S
authors.initials: S
authors.lastname: Klimasauskas
authors.forename: R J
authors.initials: RJ
authors.lastname: Roberts
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D003596:Cytosine; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D015257:DNA-Cytosine Methylases / Q000378:metabolism*; D006147:Guanine; D008969:Molecular Sequence Data; D013379:Substrate Specificity
chemical_list: D006147:Guanine; D003596:Cytosine; D004247:DNA; C037664:DNA modification methylase HhaI; D015257:DNA-Cytosine Methylases