title: Immunocytochemical study of the ontogeny of the marsupial Didelphis albiventris immune system.
abstract: Marsupials have considerable merits as models for studying the developmental dynamics of the mammalian immune system, but until recently there has been a conspicuous lack of specific immune probes to facilitate such studies. To begin a precise study of the ontogeny of the marsupial Didelphis albiventris we have used cross-reactive polyclonal antibodies raised against evolutionarily highly conserved peptides which form part of the antigen specific receptor complexes of human differentiated lymphocytes. Moreover, because of antigen receptor conservation, the antibodies also recognise specifically the immunocompetent T and B lymphocytes of other species including those in the organs of the opossum. Use of the antipeptide antibodies together with other cross-reacting antibodies has allowed us to study the cellular immunology of T and B cells and antigen presenting cells (APC) during the development of thymus, skin, lymph nodes and spleen in the Brazilian white-belly opossum. The molecular nature and identity of the T cell antigens detected in opossum tissues were confirmed by immunoblotting. These findings indicate that it is now possible to exploit these antibody probes for comparative mammalian studies, and indeed to investigate interesting features of the opossum, such as reaction of the immature immune system of the pouch young to antigenic stimulation.
authors.affiliation: Centro de Pesquisas Aggeu Magalhäes/FIOCRUZ, Recife, Brazil.
authors.forename: H B
authors.initials: HB
authors.lastname: Coutinho
authors.forename: H F
authors.initials: HF
authors.lastname: Sewell
authors.forename: P
authors.initials: P
authors.lastname: Tighe
authors.forename: G
authors.initials: G
authors.lastname: King
authors.forename: J C
authors.initials: JC
authors.lastname: Nogueira
authors.forename: T I
authors.initials: TI
authors.lastname: Robalinho
authors.forename: V B
authors.initials: VB
authors.lastname: Coutinho
authors.forename: V M
authors.initials: VM
authors.lastname: Cavalcanti
mesh_terms: D000818:Animals; D000906:Antibodies; D000938:Antigen-Presenting Cells / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D017252:CD3 Complex / Q000032:analysis; D006684:HLA-DR Antigens / Q000032:analysis; D007107:Immune System / Q000254:growth & development; D007111:Immunity, Cellular; D015151:Immunoblotting; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000254:growth & development; D008643:Mesentery; D009893:Opossums / Q000254:growth & development / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D012867:Skin / Q000254:growth & development; D013154:Spleen / Q000254:growth & development; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000254:growth & development
chemical_list: D000906:Antibodies; D017252:CD3 Complex; D006684:HLA-DR Antigens; D010455:Peptides