Contribution of the TCR alpha-chain to the differential recognition of bacterial and retroviral superantigens.
The TCR alpha-chain has been implicated in superantigen recognition by some T cells. In this study, recognition of a retroviral (minor lymphocyte stimulating Ag-1, Mls-1) and a bacterial (staphylococcal enterotoxin B, SEB) superantigen by T cells expressing an identical (transgenic) V beta 8.1 beta-chain and either a V alpha 2+ or V alpha 11+ alpha-chain have been compared. Individual hybridomas were found to vary in their recognition of the two superantigens. Whereas some T cell hybridomas recognized both superantigens comparably, other hybridomas preferentially recognized either Mls-1 or SEB, indicating that the contribution of the TCR alpha-chain to recognition of these superantigens is not equivalent. The degree of heterogeneity in recognition varied between the V alpha 2+ and V alpha 11+ hybridomas, although there were individual hybridomas within each group with distinct patterns of reactivity that varied only in CDR3 alpha. These data are consistent with recent evidence that recognition of Mls-1 and SEB involves distinct TCR/MHC interactions.
Daly
K
K
Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
Nguyen
P
P
Hankley
D
D
Zhang
W J
WJ
Woodland
D L
DL
Blackman
M A
MA
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D001483:Base Sequence; D004768:Enterotoxins / Q000276:immunology*; D005434:Flow Cytometry; D006825:Hybridomas / Q000276:immunology; D007376:Interleukin-2 / Q000032:analysis; D051379:Mice; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D016747:Minor Lymphocyte Stimulatory Antigens / Q000276:immunology*; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*; D013210:Staphylococcus / Q000276:immunology*; D018089:Superantigens / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D004768:Enterotoxins; D007376:Interleukin-2; D016747:Minor Lymphocyte Stimulatory Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens; C031818:enterotoxin B, staphylococcal