title: Protein transfer of preformed MHC-peptide complexes sensitizes target cells to T cell cytolysis.
abstract: Recombinant GPI-anchored HLA-A2.1 (HLA-A2.1-GPI/beta 2m) was used as a protein transfer vehicle to deliver a hepatitis B virus antigenic peptide to the surfaces of cytotoxic T cell targets. Empty HLA-A2.1-GPI/beta 2m was first produced in D. melanogaster cotransfectants and immunoaffinity purified. Cell coating with HLA-A2.1-GPI/beta 2m was shown to occur rapidly, and to be protein concentration dependent. Protein-transferred HLA-A2.1-GPI/beta 2m effectively presented a hepatitis B virus peptide to peptide-specific HLA-A2.1-restricted T cell clones in cytotoxicity assays. Protein transfer of functional GPI-modified class I MHC-antigenic peptide complexes represents a novel strategy for delivering functional antigenic complexes to cell surfaces that bypasses limitations of gene transfer and permits control of antigenic peptide densities at cell surfaces.
authors.affiliation: Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106, USA.
authors.forename: J H
authors.initials: JH
authors.lastname: Huang
authors.forename: R R
authors.initials: RR
authors.lastname: Getty
authors.forename: F V
authors.initials: FV
authors.lastname: Chisari
authors.forename: P
authors.initials: P
authors.lastname: Fowler
authors.forename: N S
authors.initials: NS
authors.lastname: Greenspan
authors.forename: M L
authors.initials: ML
authors.lastname: Tykocinski
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D000938:Antigen-Presenting Cells / Q000378:metabolism* / Q000648:ultrastructure; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D004306:Dose-Response Relationship, Immunologic; D004331:Drosophila melanogaster; D017261:Glycosylphosphatidylinositols / Q000235:genetics / Q000378:metabolism; D015789:HLA-A2 Antigen / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D006518:Hepatitis Viruses / Q000276:immunology; D006801:Humans; D051379:Mice; D010455:Peptides / Q000378:metabolism* / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism*; D014162:Transfection; D014759:Viral Envelope Proteins / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000378:metabolism
chemical_list: D017261:Glycosylphosphatidylinositols; D015789:HLA-A2 Antigen; D010455:Peptides; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins; D001613:beta 2-Microglobulin