rRNA operon multiplicity in Escherichia coli and the physiological implications of rrn inactivation.
Here we present evidence that only five of the seven rRNA operons present in Escherichia coli are necessary to support near-optimal growth on complex media. Seven rrn operons are necessary, however, for rapid adaptation to nutrient and temperature changes, suggesting it is the ability to adapt quickly to changing environmental conditions that has provided the selective pressure for the persistence of seven rrn operons in E. coli. We have also found that one consequence of rrn operon inactivation is a miscoordination of the concentrations of initiation factor IF3 and ribosomes.
Condon
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Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
Liveris
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Squires
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Schwartz
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Squires
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D000222:Adaptation, Physiological / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D018628:Gene Dosage; D005810:Multigene Family / Q000235:genetics*; D009876:Operon / Q000235:genetics*; D010448:Peptide Initiation Factors / Q000378:metabolism; D039665:Prokaryotic Initiation Factor-3; D012335:RNA, Ribosomal / Q000235:genetics*; D012269:Ribosomal Proteins / Q000378:metabolism; D012641:Selection, Genetic
D004275:DNA, Ribosomal; D010448:Peptide Initiation Factors; D039665:Prokaryotic Initiation Factor-3; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins