Targeted and random mutagenesis of the Campylobacter coli chromosome with integrational plasmid vectors.
A number of integrational vectors were developed for use as genetic tools in the food-borne pathogen Campylobacter coli. Integration of the plasmids occurred following genetic recombination via a Campbell-like mechanism. For an integrative plasmid containing a DNA fragment internal to the C. coli catalase gene, the insertion was mutagenic and led to a catalase-deficient phenotype. A procedure for generating random mutations in the C. coli chromosome, with these suicide-plasmids, was developed. In addition, the construction and utility of an integrable plasmid for generating transcriptional fusions to a cat reporter gene is described.
Dickinson
J H
JH
Institute of Food Research, Reading Laboratory, UK.
Grant
K A
KA
Park
S F
SF
D001377:Azaserine / Q000494:pharmacology; D001483:Base Sequence; D017000:Campylobacter coli / Q000187:drug effects / Q000235:genetics*; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D017931:DNA Primers; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010957:Plasmids; D012641:Selection, Genetic; D014158:Transcription, Genetic
D017931:DNA Primers; D001377:Azaserine; D015500:Chloramphenicol O-Acetyltransferase