title: Genetic analysis of herpes simplex virus type 1 isolates from recurrent lesions and clinical reinfections.
abstract: A study of the genetic variability of herpes simplex virus (HSV) type 1 from recurrent lesions and clinical reinfections was done using restriction endonuclease analysis and the RNase A mismatch cleavage method. Comparative genetic analyses of HSV-1 recurrent isolates from 1 patient and of HSV-1 isolates from different anatomic areas (vagina and lip) from another patient showed differences only in the glycoprotein B gene but not in the thymidine kinase gene even though the viruses had the same restriction endonuclease pattern. These results suggest the RNase A mismatch cleavage method is useful for epidemiologic studies of DNA viruses.
authors.affiliation: Centro Nacional de Biologia Celular y Retrovirus, Instituto de Salud Carlos III, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Rojas
authors.forename: S
authors.initials: S
authors.lastname: Sánchez-Palomino
authors.forename: M
authors.initials: M
authors.lastname: Santana
authors.forename: C
authors.initials: C
authors.lastname: López-Galíndez
authors.forename: E
authors.initials: E
authors.lastname: Tabarés
mesh_terms: D005260:Female; D006561:Herpes Simplex / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000235:genetics*; D006801:Humans; D012008:Recurrence; D012259:Ribonuclease, Pancreatic / Q000494:pharmacology; D013937:Thymidine Kinase / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics
chemical_list: D014759:Viral Envelope Proteins; C050591:glycoprotein gG-1, herpes simplex virus type 1; D013937:Thymidine Kinase; D012259:Ribonuclease, Pancreatic