title: TAP1-independent loading of class I molecules by exogenous viral proteins.
abstract: Presentation of peptides derived from endogenous proteins on class I molecules needs functional TAP peptide transporters. To reveal whether class I-associated presentation of exogenous proteins also required the presence of TAP transporters, we assessed in vitro the ability of spleen cells and macrophages from TAP1-deficient mice (TAP1-/-) to present peptides derived from exogenous recombinant viral proteins on their class I molecules. We found that recombinant glyco- and nucleoprotein from lymphocytic choriomeningitis virus and nucleoprotein of vesicular stomatitis virus were presented as efficiently by TAP1-/- cells as by control cells. Peptide regurgitation was not involved. Since particulate, non-replicating antigens can efficiently prime anti-viral cytotoxic T cells in vivo, this new, TAP-independent pathway of class I-associated antigen presentation may be applicable for vaccine strategies.
authors.affiliation: Department of Pathology, University of Zürich, Switzerland.
authors.forename: M F
authors.initials: MF
authors.lastname: Bachmann
authors.forename: A
authors.initials: A
authors.lastname: Oxenius
authors.forename: H
authors.initials: H
authors.lastname: Pircher
authors.forename: H
authors.initials: H
authors.lastname: Hengartner
authors.forename: P A
authors.initials: PA
authors.lastname: Ashton-Richardt
authors.forename: S
authors.initials: S
authors.lastname: Tonegawa
authors.forename: R M
authors.initials: RM
authors.lastname: Zinkernagel
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D002478:Cells, Cultured; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism*; D013154:Spleen / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology* / Q000378:metabolism
chemical_list: D015395:Histocompatibility Antigens Class I; D009842:Oligopeptides; D014764:Viral Proteins; C080396:trypsinogen activation peptide