An approach towards surface imprinting using the enzyme ribonuclease A.
An adsorbent showing enhanced selectivity for the enzyme RNase A was prepared by a surface imprinting procedure based on metal coordination. A metal chelating monomer, N-(4-vinyl)-benzyl iminodiacetic acid, was polymerized onto methacrylate-derivatized silica particles in the presence of RNase A and metal ions. Lysozyme and RNase A were separated on the adsorbent used as stationary phase in high-performance liquid chromatography.
Kempe
M
M
University of Lund, Sweden.
Glad
M
M
Mosbach
K
K
D000327:Adsorption; D000818:Animals; D002417:Cattle; D002614:Chelating Agents / Q000138:chemical synthesis; D002851:Chromatography, High Pressure Liquid; D007098:Imino Acids / Q000138:chemical synthesis; D009682:Magnetic Resonance Spectroscopy; D009113:Muramidase / Q000302:isolation & purification*; D011487:Protein Conformation; D012259:Ribonuclease, Pancreatic / Q000737:chemistry* / Q000302:isolation & purification*; D013343:Styrenes / Q000138:chemical synthesis; D013499:Surface Properties
D002614:Chelating Agents; D007098:Imino Acids; C094450:N-(4-vinyl)benzyliminodiacetic acid; D013343:Styrenes; D012259:Ribonuclease, Pancreatic; D009113:Muramidase