title: In vitro inhibition of Helicobacter pylori NCTC 11637 by organic acids and lactic acid bacteria.
abstract: In this study the effects of both pH and organic acids on Helicobacter pylori NCTC 11637 were tested. Lactobacillus acidophilus, Lact. casei, Lact. bulgaricus, Pediococcus pentosaceus and Bifidobacterium bifidus were assayed for their lactic acid production, pH and inhibition of H. pylori growth. A standard antimicrobial plate well diffusion assay was employed to examine inhibitory effects. Lactic, acetic and hydrochloric acids demonstrated inhibition of H. pylori growth in a concentration-dependent manner with the lactic acid demonstrating the greatest inhibition. This inhibition was due both to the pH of the solution and its concentration. Six strains of Lact. acidophilus and one strain of Lact. casei subsp. rhamnosus inhibited H. pylori growth where as Bifidobacterium bifidus, Ped. pentosaceus and Lact. bulgaricus did not. Concentrations of lactic acid produced by these strains ranged from 50 to 156 mmol l-1 and correlated with H. pylori inhibition. The role of probiotic organisms and their metabolic by-products in the eradication of H. pylori in vivo remains to be determined.
authors.affiliation: Department of Microbiology, Monash Medical Centre, Clayton, Victoria, Australia.
authors.forename: P D
authors.initials: PD
authors.lastname: Midolo
authors.forename: J R
authors.initials: JR
authors.lastname: Lambert
authors.forename: R
authors.initials: R
authors.lastname: Hull
authors.forename: F
authors.initials: F
authors.lastname: Luo
authors.forename: M L
authors.initials: ML
authors.lastname: Grayson
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D019342:Acetic Acid; D000898:Antibiosis; D001644:Bifidobacterium / Q000502:physiology; D016480:Helicobacter pylori / Q000187:drug effects / Q000254:growth & development*; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000096:biosynthesis / Q000494:pharmacology*; D019344:Lactic Acid; D007778:Lactobacillus / Q000502:physiology*; D009994:Osmolar Concentration; D010376:Pediococcus / Q000502:physiology
chemical_list: D000085:Acetates; D007773:Lactates; D019344:Lactic Acid; D019342:Acetic Acid