Designing of thermosensitive liposomes from natural lipids for multimodality cancer therapy.
Thermosensitive liposomes prepared from synthetic lipids such as dipalmitoyl phosphatidylcholine, distearoyl phosphatidyl choline and cholesterol (Ch) have been tried for local drug release in response to hyperthermia for achieving tumour drug targeting. Herein we report a novel method of preparation of temperature-sensitive liposomes using natural lipids egg phosphatidylcholine (PC):Ch (7:1 molar ratio) and ethanol 6% (v/v) having a transition temperature of 43 degrees C, temperature attainable by local hyperthermic treatment of tumours. The anticancer drug decarbazine [5-(3,3'-dimethyl-1-triazino) imidazole-4-carboxamide] was entrapped in these liposomes. The in vivo efficacy of temperature-sensitive unilamellar vesicles encapsulated decarbazine in combination with hyperthermia was determined in murine fibrosarcomas. These PC:Ch liposomes are biodegradable, non-toxic and more cost effective in comparison with liposomes prepared from synthetic lipids for use in multimodality cancer therapy.
Chelvi
T P
TP
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
Ralhan
R
R
D000818:Animals; D002784:Cholesterol; D015195:Drug Design; D005354:Fibrosarcoma / Q000473:pathology / Q000628:therapy; D006358:Hot Temperature; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D008081:Liposomes / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009369:Neoplasms / Q000628:therapy*; D010713:Phosphatidylcholines; D013816:Thermodynamics
D008081:Liposomes; D010713:Phosphatidylcholines; D002784:Cholesterol