title: The effect of ureteric stenting on the function and morphology of long-term rat renal allografts.
abstract: In the development of a reliable model for chronic rejection in rat renal allografts, the effect of modifying the ureteric anastomosis was tested. Rats, tolerized by pretreatment with two donor blood transfusions under Cyclosporin A, received renal allografts with either sewn or stented ureter. Control groups received isografts or underwent uninephrectomy with insertion of ureteric stents. For the first 6 days after transplantation, serum creatinine and urea values were lower in allograft recipients with stented ureters than in the group with sewn ureters. The method of ureteric anastomosis did not affect the long-term incidence of abnormal function. Allograft morphology was extremely variable from minor to extensive tubular atrophy, interstitial fibrosis, glomerular hypertrophy, focal and segmental glomerulosclerosis as well as vascular changes. Glomerulosclerosis was absent in controls and increased with time in the allografts. Two hundred days after transplantation all allograft recipients with sewn ureters exhibited some glomerulosclerosis, in half of these kidneys more than 25% of glomeruli were affected. Only 33% recipients of allografts with stented ureters exhibited some glomerulosclerosis and less than 20% of glomeruli were affected. The stented ureteric anastomosis provides a reliable method, a reduction of the technical failure rate, a reduction of the incidence of hydronephrosis, allows more accurate assessment of early renal function and may be of importance in reducing the occurrence and prevalence of glomerulosclerosis in the long-term allografts.
authors.affiliation: Monash University Department of Surgery, Monash Medical Centre, Clayton, Victoria, Australia.
authors.forename: P
authors.initials: P
authors.lastname: Jablonski
authors.forename: K
authors.initials: K
authors.lastname: Baxter
authors.forename: B O
authors.initials: BO
authors.lastname: Howden
authors.forename: V C
authors.initials: VC
authors.lastname: Marshall
authors.forename: A
authors.initials: A
authors.lastname: Stein-Oakley
authors.forename: N M
authors.initials: NM
authors.lastname: Thomson
mesh_terms: D000818:Animals; D001803:Blood Transfusion; D005500:Follow-Up Studies; D006084:Graft Rejection; D006085:Graft Survival; D016030:Kidney Transplantation / Q000379:methods / Q000473:pathology* / Q000502:physiology*; D008297:Male; D009392:Nephrectomy; D051381:Rats; D015607:Stents; D014184:Transplantation, Homologous; D014513:Ureter / Q000601:surgery*