title: A three nucleotide signature sequence in small subunit rRNA divides human Giardia in two different genotypes.
abstract: The nucleotide sequence of the 16S rRNA gene and the space DNA region was determined for Giardia duodenalis, obtained from humans in The Netherlands (AMC-4) and Washington State (CM). These rDNA sequences differ from other G. duodenalis isolates (Portland-1 and BRIS/83/HEPU/ 106) both of which have virtually identical rDNA sequences. The most characteristic feature was found close to the 5' end of the 16S rRNA. The Portland-1 -Bris/83/HEPU/ 106 type has GCG in position 22-24, while AMC-4 and CM have AUC in this position. These two sequences, present in an otherwise conserved region of the 16S rRNA, are "signature" sequences, which divide Giardia isolates into two different groups.
authors.affiliation: Department of Biology, Cleveland State University, Ohio 44115, USA.
authors.forename: H
authors.initials: H
authors.lastname: van Keulen
authors.forename: W L
authors.initials: WL
authors.lastname: Homan
authors.forename: S L
authors.initials: SL
authors.lastname: Erlandsen
authors.forename: E L
authors.initials: EL
authors.lastname: Jarroll
mesh_terms: D000818:Animals; D001483:Base Sequence; D001530:Belgium; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D017125:Genes, Protozoan; D005838:Genotype; D005872:Giardia / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D009426:Netherlands; D011044:Poland; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014861:Washington
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S