Inhibition of the effector phase of IgE-mediated allergies by tolerogenic conjugates of allergens and monomethoxypolyethylene glycol.
In this study we established that the conjugate of ovalbumin (OVA), which was used as a model allergen, with monomethoxypolyethylene glycol (mPEG), i.e., OVA(mPEG)11, was not only tolerogenic and essentially non-allergenic, but also capable of inactivating mast cells sensitized with anti-OVA IgE antibodies (Abs). Moreover, mast cells sensitized with a mixture of anti-OVA and anti-DNP IgE Abs were also desensitized by OVA(mPEG)11 with respect to both sensitivities. Most importantly, treatment of OVA-sensitive mice by OVA(mPEG)11 protected them from systemic anaphylaxis on challenge with OVA. The possibility of inactivating IgE-sensitized mast cells with mPEG conjugates of single epitopes of a given allergen is being investigated.
Bitoh
S
S
Immunology Department, University of Manitoba, Winnipeg, Canada.
Wakefield
R
R
Lang
G M
GM
Sehon
A H
AH
D000485:Allergens / Q000009:adverse effects / Q000276:immunology / Q000627:therapeutic use*; D000818:Animals; D002417:Cattle; D003888:Desensitization, Immunologic / Q000379:methods*; D004136:Dinitrobenzenes / Q000276:immunology; D007114:Immunization; D007073:Immunoglobulin E / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism; D051379:Mice; D010047:Ovalbumin / Q000009:adverse effects / Q000276:immunology / Q000627:therapeutic use; D010323:Passive Cutaneous Anaphylaxis; D011092:Polyethylene Glycols / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000276:immunology
D000485:Allergens; D004136:Dinitrobenzenes; D012710:Serum Albumin, Bovine; D012701:Serotonin; D007073:Immunoglobulin E; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol; D010047:Ovalbumin