[Genetic and molecular analysis of new recombinant t-haplotypes t(M8) and t(M9)].
The results of genetic and molecular analysis of new recombinant mouse haplotypes tM8 and tM9, derived from tw12 and t12, are described. Data on viability and fertility of tM/tM homozygotes are presented, as well as that on t-chromosome transmission ratio distortion (TRD) in the progeny of heterozygous males. Blot-hybridization analysis was performed using four t-specific probes: Tu48, Tu66, Tu119, and Tu122. Comparison of data obtained in the study with that on the structure of the initial haplotypes suggests that both recombinant haplotypes resulted from unequal crossing-over. In the case of tM9 haplotype, the crossing-over site was probably located in the region of distal t complex inversion, and tM8 haplotype resulted from two crossing-over events in the medial part of t complex.
Shustrova
I V
IV
Tokarskaia
O N
ON
Safronova
L D
LD
Ryskov
A P
AP
D000818:Animals; D005298:Fertility / Q000235:genetics; D006239:Haplotypes; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011995:Recombination, Genetic