Reversible decrease of surface beta 2-adrenoceptor number and response in lymphocytes of patients with pheochromocytoma.
To study the effect of chronic exposure to elevated plasma catecholamines on surface beta 2-adrenoceptor density, we measured these receptors in the lymphocytes of 9 patients with pheochromocytoma as well as in 27 healthy control subjects. Binding experiments were performed on intact lymphocytes using the hydrophilic ligand [3H]-CGP12177. Lymphocyte beta 2-adrenergic response was also measured in three patients. beta 2-adrenoceptor density (p < 0.01), and isoproterenol-stimulated increase in cAMP were reduced in patients with pheochromocytoma. Both parameters normalized (p < 0.05) when patients were reevaluated 4 weeks after tumor removal, coinciding with normalization of plasma epinephrine (r = -0.95, p < 0.01) and to log of plasma norepinephrine (r = -0.58, p < 0.05) in patients. We conclude that chronic catecholamine excess induces a decrease of lymphocyte beta 2-adrenoceptor surface number and response that is reversible upon normalization of plasma catecholamine levels. This regulation is mainly dependent on plasma levels of the hormone epinephrine, but norepinephrine may also play a regulatory role at supraphysiological levels.
Cases
A
A
Hypertension Research Unit, Hospital Clinic i Provincial, Barcelona, Catalonia, Spain.
Bono
M
M
Gaya
J
J
Jimenez
W
W
Calls
J
J
Esforzado
N
N
Rivera
F
F
Revert
L
L
D000310:Adrenal Gland Neoplasms / Q000097:blood*; D000319:Adrenergic beta-Antagonists; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002395:Catecholamines / Q000097:blood*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D007545:Isoproterenol; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010673:Pheochromocytoma / Q000097:blood*; D011412:Propanolamines; D011863:Radioimmunoassay; D011869:Radioligand Assay; D018343:Receptors, Adrenergic, beta-2 / Q000378:metabolism*
D000319:Adrenergic beta-Antagonists; D002395:Catecholamines; D011412:Propanolamines; D018343:Receptors, Adrenergic, beta-2; D007545:Isoproterenol; C033394:CGP 12177