Analysis of the iron-sulfur clusters within the complex I (NADH:ubiquinone oxidoreductase) isolated from potato tuber mitochondria.
The mitochondrial complex I (NADH:ubiquinone oxidoreductase) isolated from potato (Solanum tuberosum) has been investigated for the presence of iron-sulfur clusters. EPR spectroscopic analysis detected signals arising from clusters N1, N2, N3 and N4. Quantitation of the content of iron and sulfur within the isolated complex I showed the preparation to contain 22.6 mol acid-labile sulfide and 30.4 mol iron/mol complex I. The iron-sulfur cluster composition of the plant complex I appears to be similar to the well-known composition found in Neurospora crassa.
Lin
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Institut für Genbiologische Forschung, Berlin, Germany.
Sled
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VD
Ohnishi
T
T
Brennicke
A
A
Grohmann
L
L
D004578:Electron Spin Resonance Spectroscopy; D042967:Electron Transport Complex I; D007501:Iron / Q000032:analysis*; D008928:Mitochondria / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis* / Q000302:isolation & purification; D011198:Solanum tuberosum / Q000201:enzymology*; D013455:Sulfur / Q000032:analysis*
D013455:Sulfur; D007501:Iron; D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I