Comet assay to measure DNA damage in apoptotic cells.
We have used microelectrophoresis technique to study DNA fragmentation in tumor cells undergoing apoptosis induced by NK cells through ADCC. The DNA damage in target cells is proportionate to the time of co-culture with the effector cells. The assay is simple, rapid and inexpensive.
Gopalakrishna
P
P
Centre for Cellular and Molecular Biology, Hyderabad, India.
Khar
A
A
D000818:Animals; D017209:Apoptosis / Q000235:genetics*; D004249:DNA Damage; D004586:Electrophoresis; D007694:Killer Cells, Natural / Q000473:pathology; D008832:Microchemistry; D015203:Reproducibility of Results; D013997:Time Factors; D014407:Tumor Cells, Cultured