Cell composition and co-stored peptides in the endocrine pancreas of Rana arvalis.
The endocrine pancreas of Rana arvalis studied by immunochemistry consisted of islets and diffuse endocrine cells. The islets showed a mammalian-like arrangement with a central core of B cells and a peripheral mantle of A/PP cells. A few D and vasoactive intestinal polypeptide cells were also present. Consecutive sections or double-labeling studies allowed us to detect several regulatory peptides colocalized in the same endocrine cells. The so-called A/PP cells contained subpopulations of cells showing various types of immunoreactivity and varying degrees of immunolabeling. Generally, glucagon/pancreatic polypeptide, glucagon/pancreatic polypeptide/phe-met-arg-phe-amide, glucagon/pancreatic polypeptide/peptide tyrosine tyrosine, glucagon/pancreatic polypeptide/peptide tyrosine tyrosine/phe-met-arg-phe-amide immunoreactivities were present in the islets, while peptide tyrosine tyrosine/neuropeptide tyrosine colocalization was also found in the parenchyma.
Putti
R
R
Dipartimento di Biologia evolutiva e comparata, Università Federico II, Napoli, Italy.
Della Rossa
A
A
Tagliafierro
G
G
Maglio
M
M
D000595:Amino Acid Sequence; D000818:Animals; D019835:FMRFamide; D005934:Glucagon / Q000032:analysis; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000737:chemistry* / Q000166:cytology*; D008297:Male; D008969:Molecular Sequence Data; D009478:Neuropeptide Y; D009479:Neuropeptides / Q000032:analysis; D010455:Peptides / Q000032:analysis*; D011898:Ranidae / Q000378:metabolism*
D009478:Neuropeptide Y; D009479:Neuropeptides; D010455:Peptides; D019835:FMRFamide; D005934:Glucagon