title: Silica-induced micronuclei and chromosomal aberrations in Chinese hamster lung (V79) and human lung (Hel 299) cells.
abstract: Silica is one of the most abundant and widely used mineral groups. A large number of workers are potentially exposed to one or more forms of silica. Therefore, the potential carcinogenic hazard of silica to the exposed workers is of great concern. This study examines the genotoxic potential of silica with the micronucleus and chromosomal aberration assays using cultured Chinese hamster lung fibroblasts (V79) and human embryonic lung (Hel 299) cells. One-day-old cultures were treated with two types of silica, Min-U-Sil 5 and Min-U-Sil 10, for 24 h at concentrations of 40, 80, 160 and 320 micrograms/cm2. Both Min-U-Sils at 160 and 320 micrograms/cm2 induced micronucleus formation in V79 and Hel 299 cells. In V79 cells, a significant increase in the micronucleus frequency was also found with 40 and 80 micrograms/cm2. However, the chromosomal aberration frequency was unaffected by either Min-U-Sil 5 or 10 treatment of V79 or Hel 299 cells. Results indicated that silica, in different particle sizes, was capable of inducing micronuclei but not chromosomal aberrations in cultured animal and human lung cells and suggested that V79 cells were relatively more sensitive to silica than Hel 299 cells.
authors.affiliation: Genetics and Developmental Biology Program, College of Agriculture and Forestry, West Virginia University, Morgantown 26506, USA.
authors.forename: R
authors.initials: R
authors.lastname: Nagalakshmi
authors.forename: J
authors.initials: J
authors.lastname: Nath
authors.forename: T
authors.initials: T
authors.lastname: Ong
authors.forename: W Z
authors.initials: WZ
authors.lastname: Whong
mesh_terms: D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D008168:Lung / Q000187:drug effects* / Q000648:ultrastructure; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects* / Q000235:genetics / Q000648:ultrastructure; D009152:Mutagenicity Tests; D012822:Silicon Dioxide / Q000633:toxicity*
chemical_list: D012822:Silicon Dioxide