title: Multichannel PCR and serial transfer machine as a future tool in evolutionary biotechnology.
abstract: As an improved strategy for producing functional macromolecules by in vitro evolutionary optimization, we propose an automated machine that can process up to 960 samples in parallel. It consists of a 960-well PCR machine with special sealed plastic reaction vessels and appropriate handling devices. We show that the heat-sealing technique does not significantly affect the activity of Taq DNA Polymerase or that of the temperature-sensitive Q beta RNA polymerase but avoids cross-contamination and evaporation of the samples. Initial experiments demonstrate the suitability of the apparatus to uniformly process the samples and to perform the thermocycling. Serial transfer of reaction products into fresh reaction solution was used to initiate further rounds of amplification as a typical experimental setup for an evolutionary biotechnology application.
authors.affiliation: Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Schober
authors.forename: N G
authors.initials: NG
authors.lastname: Walter
authors.forename: U
authors.initials: U
authors.lastname: Tangen
authors.forename: G
authors.initials: G
authors.lastname: Strunk
authors.forename: T
authors.initials: T
authors.lastname: Ederhof
authors.forename: J
authors.initials: J
authors.lastname: Dapprich
authors.forename: M
authors.initials: M
authors.lastname: Eigen
mesh_terms: D017908:Allolevivirus / Q000235:genetics; D001483:Base Sequence; D005075:Biological Evolution; D004247:DNA / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase; D005615:Freezing; D006361:Heating / Q000295:instrumentation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000295:instrumentation*; D012371:Robotics / Q000295:instrumentation; D013048:Specimen Handling / Q000379:methods
chemical_list: D004247:DNA; D004259:DNA-Directed DNA Polymerase