title: A role for interleukin-1 in epidermal differentiation: regulation by expression of functional versus decoy receptors.
abstract: Although human epidermis contains levels of interleukin-1 (IL-1) up to 100 times higher than other tissues, the role of this cytokine in epidermal biology is unknown. Here, we show that interleukin-1 regulates the expression of mRNAs for two proteins associated with the differentiated phenotype of human keratinocytes, cellular retinoic acid-binding protein type II (CRABP II) and small, proline rich protein 1 (SPRR1). The ability of IL-1 to induce these transcripts correlates directly with keratinocyte expression of the IL-1 receptor type I (IL-1 RI) during differentiation and inversely with the expression of the type II IL-1 receptor (IL-1 RII), shown in other cell types to be a nonfunctional, decoy receptor. Furthermore, addition to keratinocyte cultures of an IL-1 RI-blocking, but not an IL-1 RII-blocking, antibody reduces the levels of CRABP II and SPRR1 mRNAs in these cells. These data suggest that epidermal IL-1 functions to promote keratinocyte differentiation and that a change in the IL-1 receptor profile of these cells initiates this IL-1 response through a relative enhanced expression of functional IL-1 receptors.
authors.affiliation: Boston University School of Medicine, Department of Dermatology, MA, USA.
authors.forename: M S
authors.initials: MS
authors.lastname: Eller
authors.forename: M
authors.initials: M
authors.lastname: Yaar
authors.forename: K
authors.initials: K
authors.lastname: Ostrom
authors.forename: D D
authors.initials: DD
authors.lastname: Harkness
authors.forename: B A
authors.initials: BA
authors.lastname: Gilchrest
mesh_terms: D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D055233:Cornified Envelope Proline-Rich Proteins; D017931:DNA Primers; D015342:DNA Probes; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007231:Infant, Newborn; D007375:Interleukin-1 / Q000494:pharmacology*; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D008297:Male; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010641:Phenotype; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D017472:Receptors, Interleukin-1 / Q000096:biosynthesis*; D018168:Receptors, Retinoic Acid / Q000096:biosynthesis*; D012867:Skin / Q000166:cytology* / Q000187:drug effects; D012879:Skin Physiological Phenomena; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D055233:Cornified Envelope Proline-Rich Proteins; D017931:DNA Primers; D015342:DNA Probes; D007375:Interleukin-1; D008565:Membrane Proteins; D011506:Proteins; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D018168:Receptors, Retinoic Acid; C082928:retinoic acid binding protein II, cellular