title: Effect of copper depletion and repletion on lymphocyte blastogenesis and neutrophil bactericidal function in beef heifers.
abstract: Thirty-two beef heifers were used to examine the effect of dietary copper depletion and repletion on neutrophil and lymphocyte functions. Heifers allotted to the control group (C+; n = 8) were fed a basal roughage/concentrate diet with Cu-sulfate supplementation (Cu > or = 8 ppm). To induce a Cu deficiency (depletion phase d 0 to 60), treated (T; n = 24) heifers received a diet supplemented with sulfur (.3% of diet) and sodium molybdate to achieve a Cu:Mo ratio of 1:1.5. Liver biopsies were collected on d 0, 27, and 60. Despite random allocation, T heifers had lower initial liver Cu concentrations (P < .01) than C+ heifers. At the start of the repletion phase (d 0, equal to d 60 of depletion), treated heifers were allotted by liver Cu concentration to three treatments (n = 8/treatment): Cu sulfate (S; Cu = 10 ppm), Cu proteinate (P; Cu = 10 ppm), or a negative control (C-) that remained on Mo and S supplementation. During the repletion phase, livers were biopsied on d 0, 14, and 45. By d 45, both S and P heifers had greater (P < .05) liver Cu concentrations than C- heifers. For both depletion and repletion phases, no treatment differences were detected in liver Mo or S concentrations. Jugular blood was collected on d 0, 27, and 55 of the depletion phase and d 0, 13, and 42 of the repletion phase. Neutrophils were isolated and incubated with Staphylococcus aureus to determine neutrophil bactericidal capacity (NBC).(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Animal Sciences and Industry, Kansas State University, Manhattan 66506-0201, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Arthington
authors.forename: L R
authors.initials: LR
authors.lastname: Corah
authors.forename: F
authors.initials: F
authors.lastname: Blecha
authors.forename: D A
authors.initials: DA
authors.lastname: Hill
mesh_terms: D000818:Animals; D001706:Biopsy; D002417:Cattle / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003300:Copper / Q000172:deficiency / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D005527:Food, Fortified; D006400:Hematocrit; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D010835:Phytohemagglutinins / Q000494:pharmacology; D011897:Random Allocation; D013211:Staphylococcus aureus / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D015430:Weight Gain / Q000502:physiology
chemical_list: D010835:Phytohemagglutinins; D014316:Tritium; D003208:Concanavalin A; D003300:Copper; D013936:Thymidine