Rapid processing of filter-grown cells for Epon embedding.
A simple and rapid method for fixation, processing, and Epon embedding of cells grown on a filter support is described. The results show that it is possible to embed filter-grown cells in resin within 30 min of applying the primary fixative and still to achieve good ultrastructural preservation.
Parton
R G
RG
European Molecular Biology Laboratory, Heidelberg, Germany.
D000818:Animals; D002478:Cells, Cultured; D004285:Dogs; D004853:Epoxy Resins; D007668:Kidney / Q000648:ultrastructure; D008854:Microscopy, Electron; D016613:Plastic Embedding / Q000379:methods*
D004853:Epoxy Resins; C004875:EPON