title: Amino acid sequence heterogeneity of the chromosomal encoded Borrelia burgdorferi sensu lato major antigen P100.
abstract: The entire nucleotide sequence of the chromosomal encoded major antigen p100 of the European Borrelia garinii isolate B29 was determined and the deduced amino acid sequence was compared to the homologous antigen p83 of the North American Borrelia burgdorferi sensu stricto strain B31 and the p100 of the European Borrelia afzelii (group VS461) strain PKo. p100 of strain B29 shows 87% amino acid sequence identity to strain B31 and 79.2% to strain PKo, p100 of strain B31 and PKo shows 62.5% identity to each other. In addition, partial nucleotide sequences of the most heterogeneous region of the p100 gene of two other Borrelia garinii isolates (PBi and VS286) have been determined and the deduced amino acid sequences were compared with all p100 of Borrelia garinii published so far. We found an amino acid sequence identity between 88.6 and 100% within the same genospecies. The N-terminal part of the p100 proteins is highly conserved whereas a striking heterogeneous region within the C-terminal part of the proteins was observed.
authors.affiliation: Institut für Mikrobiologie (Med. Fak.), Universität Innsbruck, Austria.
authors.forename: W
authors.initials: W
authors.lastname: Fellinger
authors.forename: A
authors.initials: A
authors.lastname: Farencena
authors.forename: B
authors.initials: B
authors.lastname: Redl
authors.forename: V
authors.initials: V
authors.lastname: Sambri
authors.forename: R
authors.initials: R
authors.lastname: Cevenini
authors.forename: G
authors.initials: G
authors.lastname: Stöffler
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D001898:Borrelia / Q000235:genetics*; D015748:Borrelia burgdorferi Group / Q000235:genetics; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C085289:p100 protein, Borrelia