Synergistic induction of the heat shock response in Escherichia coli by simultaneous treatment with chemical inducers.
Escherichia coli strains carrying transcriptional fusions of four sigma 32-controlled E. coli heat shock promoters to luxCDABE or lacZ reporter genes were stressed by chemicals added singly or in pairs. Much more than additive induction resulted from combinations of cadmium chloride, copper sulfate, ethanol, formamide, 4-nitrophenol, and pentachlorophenol.
Van Dyk
T K
TK
DuPont Company, Wilmington, Delaware 19880-0173, USA.
Reed
T R
TR
Vollmer
A C
AC
LaRossa
R A
RA
D001483:Base Sequence; D002104:Cadmium / Q000494:pharmacology; D019256:Cadmium Chloride; D002712:Chlorides / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D019327:Copper Sulfate; D004357:Drug Synergism; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D000431:Ethanol / Q000494:pharmacology; D005559:Formamides / Q000494:pharmacology; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D018869:Heat-Shock Response / Q000502:physiology*; D007763:Lac Operon; D008156:Luciferases / Q000235:genetics; D008163:Luminescent Measurements; D008242:Lysogeny / Q000235:genetics; D008969:Molecular Sequence Data; D009596:Nitrophenols / Q000494:pharmacology; D010416:Pentachlorophenol / Q000494:pharmacology; D010957:Plasmids / Q000032:analysis; D011401:Promoter Regions, Genetic / Q000235:genetics
D002712:Chlorides; D005559:Formamides; D009596:Nitrophenols; D002104:Cadmium; D000431:Ethanol; C031066:formamide; D003300:Copper; D010416:Pentachlorophenol; D008156:Luciferases; D019256:Cadmium Chloride; D019327:Copper Sulfate; C024836:4-nitrophenol