title: Activation of a novel form of phospholipase A2 during liver regeneration.
abstract: Activation of phospholipase A2 (PLA2) occurs following mitogenic stimulation of cells. This study examined PLA2 activation during liver regeneration. Increased activity was detected within 1 h after partial hepatectomy, was maximal by 6 h, and returned to control levels by 24 h. Fractionation of cell-free extracts revealed multiple peaks of PLA2 activity. One peak appeared identical to the previously described cPLA2, and was modestly stimulated during regeneration. A higher molecular weight form (hPLA2) was stimulated approximately 5-fold during regeneration. This enzyme was Ca(2+)-dependent and selective for arachidonoylphosphatidylethanolamine. The activation of this novel form of PLA2 represents an early event in liver regeneration, and is likely to contribute to the proliferative response.
authors.affiliation: Department of Medicine, University of Colorado School of Medicine, Denver 80262, USA.
authors.forename: P A
authors.initials: PA
authors.lastname: Wojtaszek
authors.forename: V
authors.initials: V
authors.lastname: Van Putten
authors.forename: R A
authors.initials: RA
authors.lastname: Nemenoff
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent; D004789:Enzyme Activation; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D010741:Phospholipases A / Q000037:antagonists & inhibitors / Q000378:metabolism*; D054467:Phospholipases A2; D051381:Rats; D017207:Rats, Sprague-Dawley; D013379:Substrate Specificity
chemical_list: D002413:Cations, Divalent; D007527:Isoenzymes; D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium