Assessment of mitochondrial function and control in normal and diseased states.
Mitochondrial function in muscle in vivo can be quantitatively evaluated using 31-phosphorus nuclear magnetic resonance. In resting muscle, the concentrations of ions (e.g. H+, Na+) and two of the major bioenergetic components (inorganic phosphate and creatine) are determined by regulated transcellular transport processes. During recovery after exercise the kinetics and control of mitochondrial ATP synthesis can be established. During exercise the relative contributions to ATP synthesis of phosphocreatine (using creatine kinase), anaerobic glycogenolysis and oxidative phosphorylation are dissected and have been shown to change with time. The consequences of mitochondrial lesions and dysfunctions on these processes have been summarised.
Radda
G K
GK
MRC Biochemical and Clinical Magnetic Resonance Unit, Oxford Radcliffe Hospital, UK.
Odoom
J
J
Kemp
G
G
Taylor
D J
DJ
Thompson
C
C
Styles
P
P
D000255:Adenosine Triphosphate / Q000378:metabolism; D004734:Energy Metabolism; D006801:Humans; D007511:Ischemia / Q000378:metabolism; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008928:Mitochondria / Q000378:metabolism*; D017240:Mitochondrial Myopathies / Q000175:diagnosis / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism; D012016:Reference Values; D006435:Renal Dialysis; D014511:Uremia / Q000378:metabolism / Q000628:therapy
D010725:Phosphocreatine; D000255:Adenosine Triphosphate