title: The GroES homolog of Helicobacter pylori confers protective immunity against mucosal infection in mice.
abstract: Helicobacter pylori is an important etiologic agent of gastroduodenal disease. In common with other organisms, H. pylori bacteria express heat shock proteins that share homologies with the GroES-GroEL class of proteins from Escherichia coli. We have assessed the heat shock proteins of H. pylori as potential protective antigens in a murine model of gastric Helicobacter infection. Orogastric immunization of mice with recombinant H. pylori GroES- and GroEL-like proteins protected 80% (n = 20) and 70% (n = 10) of animals, respectively, from a challenge dose of 10(4) Helicobacter felis bacteria (compared to control mice, P = 0.0042 and P = 0.0904, respectively). All mice (n = 19) that were immunized with a dual antigen preparation, consisting of H. pylori GroES-like protein and the B subunit of H. pylori urease, were protected against infection. This represented a level of protection equivalent to that provided by a sonicated Helicobacter extract (P = 0.955). Antibodies directed against the recombinant H. pylori antigens were predominantly of the IgG1 class, suggesting that a type 2 T-helper cell response was involved in protection. This work reports a protein belonging to the GroES class of heat shock proteins that was shown to induce protective immunity. In conclusion, GroES-like and urease B-subunit proteins have been identified as potential components of a future H. pylori subunit vaccine.
authors.affiliation: Unité des Entérobactéries, Institut National de la Santé et de la Recherche Médicle U389, Paris, France.
authors.forename: R L
authors.initials: RL
authors.lastname: Ferrero
authors.forename: J M
authors.initials: JM
authors.lastname: Thiberge
authors.forename: I
authors.initials: I
authors.lastname: Kansau
authors.forename: N
authors.initials: N
authors.lastname: Wuscher
authors.forename: M
authors.initials: M
authors.lastname: Huerre
authors.forename: A
authors.initials: A
authors.lastname: Labigne
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000097:blood; D018835:Chaperonin 10 / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000378:metabolism; D005753:Gastric Mucosa / Q000276:immunology* / Q000473:pathology; D016481:Helicobacter Infections / Q000276:immunology* / Q000517:prevention & control; D016480:Helicobacter pylori / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D051379:Mice; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D018835:Chaperonin 10; D007074:Immunoglobulin G; D011994:Recombinant Proteins