A mutant of Sindbis virus which is released efficiently from cells maintained in low ionic strength medium.
Experiments many years ago showed that reducing the ionic strength of the medium Sindbis virus-infected chick embryo fibroblasts were maintained prevented the release of progeny virus into the medium. It was proposed at that time that the susceptibility of virus release to inhibition by low ionic strength (LIS) medium might be determined by the properties of the viral envelope proteins. We thought that if such were the case, it might be possible to obtain a viral mutant which is resistant to the inhibitory effect of LIS medium. We report here that we were able to isolate such a mutant of Sindbis virus, and that we identified two amino acids (Glu23 and Thr76) which, when present in the E2 protein, made possible the release of infectious progeny virus from cells incubated in LIS medium.
Li
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ML
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854-5635, USA.
Stollar
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D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D002642:Chick Embryo; D006224:Cricetinae; D003470:Culture Media; D004252:DNA Mutational Analysis; D005347:Fibroblasts; D005814:Genes, Viral / Q000235:genetics; D018698:Glutamic Acid / Q000502:physiology; D009994:Osmolar Concentration; D017354:Point Mutation; D012845:Sindbis Virus / Q000235:genetics / Q000254:growth & development*; D013912:Threonine / Q000502:physiology; D014759:Viral Envelope Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics; D014776:Virus Cultivation
D003470:Culture Media; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; C064950:glycoprotein E2, Sindbis virus; D013912:Threonine; D018698:Glutamic Acid