title: Structural analysis of glycosaminoglycans derived from axonally transported proteoglycans in regenerating goldfish optic nerve.
abstract: Structural characteristics of glycosaminoglycans (GAGs) derived from axonally transported proteoglycans (PGs) were compared in 21 days regenerating and intact goldfish optic tracts. Twenty one days following unilateral optic nerve crushes, fish received intraocular injections of 35SO4. Eight hours post injection, tracts were removed and the 35SO4-labeled GAGs, chondroitin sulfate (CS) and heparan sulfate (HS), isolated. The HS from regenerating optic tracts had a DEAE elution profile indicative of decreased charge density, while heparitinase treatment of HS followed by Sephadex G50 analysis of the resulting fragments showed a change in the elution pattern, suggesting reduced overall sulfation. HPLC analysis of HS disaccharides revealed a difference in the sulfation pattern of regenerating tract HS, characterized by the reduced presence of tri-sulfated disaccharides. Other structural features, such as the sizes of CS and HS, and the sulfation of CS, showed no changes during regeneration. These results indicate that changes in the structure of axonally transported HS accompany regeneration of goldfish optic axons.
authors.affiliation: Program in Neuroscience, Florida State University, Tallahassee 32306, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: Challacombe
authors.forename: J S
authors.initials: JS
authors.lastname: Elam
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism*; D001692:Biological Transport / Q000502:physiology; D002240:Carbohydrate Sequence; D002809:Chondroitin Sulfates / Q000737:chemistry / Q000378:metabolism; D006025:Glycosaminoglycans / Q000737:chemistry*; D006054:Goldfish / Q000378:metabolism / Q000502:physiology*; D006497:Heparan Sulfate / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D009416:Nerve Regeneration / Q000502:physiology*; D009900:Optic Nerve / Q000502:physiology*; D011509:Proteoglycans / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D002809:Chondroitin Sulfates; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D011509:Proteoglycans