title: Acetylcholine esterase and peripherin mRNA level decrease in wobbler mouse.
abstract: Homozygote wobbler mice develop motoneurone degeneration. Throughout development the expression of choline acetyltransferase, of trkC receptor and F3 adhesion molecule genes is similar in wobbler and wild-type spinal cord. Acetylcholinesterase mRNA level instead is decreased to about 50% with respect to wild-type values in one forth of P5 and P10 wobbler progeny, putative wr/wr individuals; at P21 its expression is equally highly reduced in known homozygotes and it is reduced to 35% of normal values in about one half of the progeny, putative heterozygotes. Thus, similarly to medium neurofilament gene over-expression, reduced acetylcholinesterase gene expression is an early molecular marker for the wobbler mutation before onset of the illness.
authors.affiliation: International Institute of Genetics and Biophysics, CNR, Naples, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Pernas-Alonso
authors.forename: A E
authors.initials: AE
authors.lastname: Schaffner
authors.forename: C T
authors.initials: CT
authors.lastname: Hansen
authors.forename: J L
authors.initials: JL
authors.lastname: Barker
authors.forename: U
authors.initials: U
authors.lastname: di Porzio
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007381:Intermediate Filament Proteins / Q000235:genetics*; D008562:Membrane Glycoproteins; D051379:Mice; D008818:Mice, Neurologic Mutants; D008969:Molecular Sequence Data; D009046:Motor Neurons / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D009479:Neuropeptides / Q000235:genetics*; D064531:Peripherins; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis*; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics; D020812:Receptor, trkC; D017475:Receptors, Nerve Growth Factor / Q000235:genetics; D012016:Reference Values; D013116:Spinal Cord / Q000166:cytology / Q000196:embryology / Q000254:growth & development
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D064531:Peripherins; D012333:RNA, Messenger; D017475:Receptors, Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D020812:Receptor, trkC; D000110:Acetylcholinesterase