title: Gender identification of dried human bloodstains using fluorescence in situ hybridization.
abstract: Identification of the gender of an individual(s) from whom a bloodstain is derived represents important evidence in medicolegal cases. The efficacy of fluorescence in situ hybridization (FISH) using chromosome X and Y centromeric probes was tested to determine its ability to identify correctly the gender of extracted dried bloodstains. In this preliminary study, FISH correctly identified the gender of 2-week-old dried bloodstains in prepared mixtures of male-to-female blood as low as 1%. The technique is accurate, rapid, sensitive, easily performed and readily available. This application of FISH as a forensic laboratory technique holds great promise.
authors.affiliation: Department of Pediatrics, Bowman Gray School of Medicine of Wake Forest University, USA.
authors.forename: M J
authors.initials: MJ
authors.lastname: Pettenati
authors.forename: P N
authors.initials: PN
authors.lastname: Rao
authors.forename: S
authors.initials: S
authors.lastname: Schnell
authors.forename: R
authors.initials: R
authors.lastname: Hayworth-Hodge
authors.forename: P E
authors.initials: PE
authors.lastname: Lantz
authors.forename: K R
authors.initials: KR
authors.lastname: Geisinger
mesh_terms: D001801:Blood Stains; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D012732:Sex Determination Analysis / Q000379:methods*