Cloning and mapping of the mouse alpha 7-neuronal nicotinic acetylcholine receptor.
We report the isolation of cDNA clones for the mouse alpha 7 neuronal nicotinic acetylcholine receptor subunit (gene symbol Acra7), the only nicotinic receptor subunit known to bind alpha-bungarotoxin in mammalian brain. This gene may have relevance to nicotine sensitivity and to some electrophysiologic findings in schizophrenia. The mouse alpha 7 subunit gene encodes a protein of 502 amino acids with substantial identity to the rat (99.6%), human (92.8%), and chicken (87.5%) amino acid sequences. The alpha 7 gene was mapped to mouse chromosome 7 near the p locus with the following gene order from proximal to distal: Myod1-3.5 +/- 1.7 cM-Gas2-0.9 cM +/- 0.9 cM-D7Mit70-1.8 +/- 1.2 cM-Acra7-4.4 +/- 1.0 cM-Hras1-ps1/Igf1r/Snrp2a. The human gene was confirmed to map to the homologous region of human chromosome 15q13-q14.
Orr-Urtreger
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A
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, USA.
Seldin
M F
MF
Baldini
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Beaudet
A L
AL
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics; D002874:Chromosome Mapping; D016384:Consensus Sequence; D003433:Crosses, Genetic; D018076:DNA, Complementary / Q000235:genetics; D005796:Genes; D006801:Humans; D051379:Mice / Q000235:genetics*; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D009115:Muridae / Q000235:genetics; D051381:Rats / Q000235:genetics; D011978:Receptors, Nicotinic / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D018076:DNA, Complementary; D011978:Receptors, Nicotinic