Conditioned medium from a cell strain derived from a patient with mastocytosis induces the development of mature human mast cells in vitro from normal human bone marrow.
Mature human mast cells were obtained from human bone marrow in a coculture system using conditioned medium from a human mastocytosis cell strain. These cells expressed the high-affinity IgE receptor and were tryptase, chymase and carboxypeptidase positive.
Li
L
L
Department of Immunology, Allergy and Infectious Disease, St. George Hospital, Kogarah, Australia.
Macpherson
J J
JJ
Adelstein
S
S
Bunn
C L
CL
Atkinson
K
K
Phadke
K
K
Krilis
S A
SA
D015415:Biomarkers; D001854:Bone Marrow Cells; D002268:Carboxypeptidases / Q000032:analysis; D002478:Cells, Cultured; D053818:Chymases; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D006801:Humans; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects*; D008415:Mastocytosis / Q000473:pathology*; D017455:Receptors, IgE / Q000096:biosynthesis / Q000235:genetics; D012697:Serine Endopeptidases / Q000032:analysis; D053802:Tryptases
D015415:Biomarkers; D017077:Culture Media, Conditioned; D017455:Receptors, IgE; D002268:Carboxypeptidases; D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases