[Isolation and study of the properties of Streptomyces kanamyceticus mutants with disorders of kanamycin biosynthesis].
The strains of Streptomyces kanamyceticus 1375 and 1 were exposed to N-nitroso-N-methyl urea, ethidium bromide and acriflavine and mutants with impaired biosynthesis of kanamycin (Kan-) were isolated. The majority of the mutants were genetically unstable and segregated with the formation of both the Kan- and Kan+ clones. The stable Kan- mutants were tested in pairs for the cosynthesis. The capacity of the mutants for the recovery of the kanamycin synthesis in media supplemented with kanamycin precursors such as 2-desoxystreptamine and D-glucoseamine was studied. Four classes of the Kan- mutants involving various genes controlling the kanamycin biosynthesis in S. kanamyceticus were identified by the results of the tests.
Golets
L M
LM
Basiliia
L I
LI
Fedorenko
V A
VA
D003001:Cloning, Molecular; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005819:Genetic Markers; D007612:Kanamycin / Q000096:biosynthesis*; D009154:Mutation; D013302:Streptomyces / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D003470:Culture Media; D005819:Genetic Markers; D007612:Kanamycin