title: Binding sites of ZNC(C)PR, a pentapeptide fragment of argipressin, in rat brain.
abstract: OBJECTIVE
  To study the binding sites of ZNC (C) PR, a C-terminal pentapeptide fragment of argipressin, in rat brain.
  
  
  METHODS
  Using radioligand assay and radioligand [35S]ZNC(C)PR.
  
  
  RESULTS
  It was found that there exist native binding sites bound ZNC(C)PR with high affinity in a saturable, reversible and specific manner. Scatchard and kinetic analyses showed that these sites were homogeneous with a Kd value of 1.69 +/- 0.16 nmol.L-1 (in the presence of MgCl2 10 mmol.L-1). The binding of ZNC(C)PR to the sites was at a higher level in the brain regions (such as amygdala, cortex,) and was affected by Mg2+.
  
  
  CONCLUSIONS
  These binding sites represented a new type of receptors and mediated the action of ZNC(C)PR on memory.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
authors.forename: J H
authors.initials: JH
authors.lastname: Wu
authors.forename: Y C
authors.initials: YC
authors.lastname: Du
mesh_terms: D000679:Amygdala / Q000378:metabolism; D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D001665:Binding Sites; D001667:Binding, Competitive; D001921:Brain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D010446:Peptide Fragments / Q000378:metabolism*; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar
chemical_list: C094362:(phenylmethoxy)carbonyl-asparaginyl-(cysteinyl)cysteinyl-prolyl-arginine; D010446:Peptide Fragments; D001127:Arginine Vasopressin