Protoplast fusion method for efficient transformation of mouse spermatogenic cells.
The efficiencies of methods for introducing DNAs into spermatogenic cells were investigated. The calcium phosphate method has been most commonly used to transfect DNA into cultured cells, but this method severely damages spermatogenic cells. Therefore, we investigated the efficiencies of transfection of spermatogenic cells by DEAE-dextran, modified liposome, and protoplast fusion methods, which seemed to cause less damage to spermatogenic cells than the calcium phosphate method. The protoplast fusion method was the most suitable for transfection of spermatogenic cells.
Jimbo
M
M
Department of Life Science, Tokyo Institute of Technology, Yokohama.
Matsumoto
M
M
Kurata
S
S
Hoshi
M
M
D000818:Animals; D002478:Cells, Cultured; D015500:Chloramphenicol O-Acetyltransferase / Q000032:analysis / Q000096:biosynthesis*; D003637:DEAE-Dextran; D004337:Drug Carriers; D004926:Escherichia coli; D008081:Liposomes; D008297:Male; D008561:Membrane Fusion; D051379:Mice; D008813:Mice, Inbred ICR; D008815:Mice, Inbred Strains; D010957:Plasmids; D011092:Polyethylene Glycols / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D011523:Protoplasts / Q000502:physiology; D013091:Spermatogenesis; D013737:Testis / Q000166:cytology / Q000502:physiology*; D014162:Transfection / Q000379:methods*
D004337:Drug Carriers; D008081:Liposomes; D011092:Polyethylene Glycols; D003637:DEAE-Dextran; D015500:Chloramphenicol O-Acetyltransferase