Biological characteristics of a continuous cultured cell line derived from a human malignant fibrous histiocytoma.
A continuous cultured cell line, NATO, was established from a human malignant histiocytoma of bone. The cultured cells consisted of at least 3 types of tumor cells; polygonal, long spindle and occasionally giant cells, which were primarily observed in the original tumor of the patient. Ultrastructurally, they were mostly immature cells that had poorly developed cell organelles and a few lysozomal granules. The cultured cells had phagocytotic activity and were positive for acid phosphatase, transferrin receptor, alpha 1-antitrypsin, alpha-naphthyl acetate esterase and HLA-DR, but negative for Fc- and C3-receptors. Allotransplantation of the cells into athymic nude mice produced tumors at early passages, but did not 3 years later. Four cloned sublines isolated from the parent line also showed essentially an identical morphology with that of the parent cell line, indicating that the 3 cell types were interchangeable.
Sekiguchi
M
M
Department of Surgery, University of Tokyo.
Asanuma
K
K
Shiroko
Y
Y
Mori
S
S
Shimoda
T
T
Kamada
N
N
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D002648:Child; D018219:Histiocytoma, Benign Fibrous / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D007621:Karyotyping; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D010587:Phagocytosis; D014407:Tumor Cells, Cultured
D014408:Biomarkers, Tumor