Protein-free culture of the human pancreatic cancer cell line, SUIT-2.
A human pancreatic cancer cell line (SUIT-2), usually cultured in serum-supplemented medium (DMEM/FBS), was adapted to protein-free conditions using a 1:1 mixture of DMEM and Ham's F12 medium (DMEM/F12). The cells have been maintained in DMEM/F12 for more than 2 years, with over 50 passages. The SUIT-2 cells grew in DMEM/F12 with a doubling time of 35.7 h, which was similar to that in DMEM/FBS (35.0 h). The cellular morphology was similar in both media. Type IV collagenolytic activity was detected in the conditioned media from cells grown in DMEM/F12. The secretion of CEA and CA19-9 initially decreased in DMEM/F12. CEA was not detected after passage 5 (p5) but the concentration of CA19-9 did not decrease further after the first few serial passages in protein-free medium. Xenografts of SUIT-2 cells cultured in DMEM/F12 remained tumorigenic and could form metastatic tumors in nude mice. In conclusion, SUIT-2 cells grown in protein-free media continued to produce CA19-9 and type IV collagenase in vitro and formed metastatic tumors in vivo.
Taniguchi
S
S
Department of Surgery I, Miyazaki Medical College.
Iwamura
T
T
Kitamura
N
N
Yamanari
H
H
Kojima
A
A
Hidaka
K
K
Seguchi
K
K
Setoguchi
T
T
D000818:Animals; D002455:Cell Division; D003094:Collagen / Q000378:metabolism; D003470:Culture Media; D006801:Humans; D008207:Lymphatic Metastasis; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000378:metabolism / Q000473:pathology*; D011506:Proteins; D014407:Tumor Cells, Cultured
D003470:Culture Media; D011506:Proteins; D003094:Collagen