title: The mRNA-binding protein COLBP is glutamate dehydrogenase.
abstract: Expression of the liver-type isopeptides of cytochrome c oxidase is regulated post-transcriptionally. An RNA-binding activity has been found in only those cells where the liver-type subunits are detected. This binding protein, termed COLBP, recognises sequences or structures within the 3'-untranslated regions of transcripts encoding these liver-type isopeptides and has been implicated in the modulation of mRNA expression. We now show by subcellular fractionation, immunocompetition, UV-crosslinking and shift-Western studies that the metabolic enzyme glutamate dehydrogenase, previously reported as being able to bind RNA, is the cytochrome c oxidase transcript-binding protein, COLBP.
authors.affiliation: Division of Clinical Neuroscience, University of Newcastle upon Tyne, The Medical School, UK.
authors.forename: T
authors.initials: T
authors.lastname: Preiss
authors.forename: A E
authors.initials: AE
authors.lastname: Sang
authors.forename: Z M
authors.initials: ZM
authors.lastname: Chrzanowska-Lightowlers
authors.forename: R N
authors.initials: RN
authors.lastname: Lightowlers
mesh_terms: D000818:Animals; D002417:Cattle; D003576:Electron Transport Complex IV / Q000235:genetics*; D005786:Gene Expression Regulation; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000737:chemistry* / Q000378:metabolism*; D051381:Rats
chemical_list: D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D005969:Glutamate Dehydrogenase; D003576:Electron Transport Complex IV