Correlating the pharmacology and molecular biology of opioid receptors. Cloning and antisense mapping a kappa 3-related opiate receptor.
We cloned a kappa 3-related opioid receptor, and although it is still not clear whether this clone corresponds to the kappa 3 receptor itself or is a related gene product, the extensive antisense mapping and the antibody immunoreactivity strongly associate this clone with the kappa 3 receptor. Our approach also indicates the usefulness of antisense approaches in mapping and identifying orphan receptors. Perhaps it is most effective in identifying partial sequences prior to cloning them in their entirety. It also provides a mechanism of identifying proteins that are not expressed functionally.
Pasternak
G W
GW
Cotzias Laboratory of Neuro-Oncology Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Pan
Y X
YX
Cheng
J
J
D000818:Animals; D003001:Cloning, Molecular; D051379:Mice; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D017473:Receptors, Opioid, kappa / Q000187:drug effects* / Q000235:genetics / Q000276:immunology; D017450:Receptors, Opioid, mu / Q000187:drug effects
D016376:Oligonucleotides, Antisense; D017473:Receptors, Opioid, kappa; D017450:Receptors, Opioid, mu