title: The DNA repair genes RAD54 and UNG1 are cell cycle regulated in budding yeast but MCB promoter elements have no essential role in the DNA damage response.
abstract: The DNA repair genes RAD54 and UNG1 have MCB elements in their promoters and are shown to be cell cycle regulated. Their transcripts are coordinately expressed with RNR1, ribonucleotide reductase, a MCB-regulated gene known to be expressed in late G1. However, no evidence was obtained for a direct role of MCB elements in DNA repair. Of the proteins that bind and activate MCB elements, only mutations in SWI6 have a defect in DNA repair, showing significant sensitivity to methyl methane sulphonate. Furthermore, analysis of the CDC9 promoter indicates that MCB elements are not required for the induction of the gene by ultraviolet light irradiation. These promoter elements may not respond directly to DNA damage but may have a role in enhancing the induction response.
authors.affiliation: Division of Yeast Genetics, National Institute for Medical Research, London, UK.
authors.forename: L H
authors.initials: LH
authors.lastname: Johnston
authors.forename: A L
authors.initials: AL
authors.lastname: Johnson
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D004249:DNA Damage; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics*; D005656:Fungal Proteins / Q000494:pharmacology; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D012264:Ribonucleotide Reductases / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins; D015398:Signal Transduction; D014157:Transcription Factors / Q000494:pharmacology
chemical_list: C000607890:CDC9 protein, S cerevisiae; D005656:Fungal Proteins; C083209:MBP1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D008741:Methyl Methanesulfonate; D012264:Ribonucleotide Reductases; D011088:DNA Ligases; D000072481:DNA Ligase ATP