title: DNA fingerprinting by pulsed-field gel electrophoresis to investigate a nosocomial pneumonia caused by Legionella bozemanii serogroup 1.
abstract: We typed 18 isolates of Legionella bozemanii obtained from clinical and environmental sources by pulsed-field gel electrophoresis. Each of the unrelated strains showed individual restriction patterns of the genomic DNA when either the SfiI or NotI restriction enzyme was used. One strain isolated from a patient with nosocomial legionellosis and two strains from the corresponding hospital water supply were indistinguishable, arguing for a transmission of L. bozemanii from the water supply to the patient. In conclusion, macrorestriction analysis is a valuable tool for studies of the molecular epidemiology of L. bozemanii.
authors.affiliation: Institut für Medizinische Mikrobiologie und Hygiene, Technische Universität Dresden, Germany.
authors.forename: P C
authors.initials: PC
authors.lastname: Lück
authors.forename: J H
authors.initials: JH
authors.lastname: Helbig
authors.forename: H J
authors.initials: HJ
authors.lastname: Hagedorn
authors.forename: W
authors.initials: W
authors.lastname: Ehret
mesh_terms: D003428:Cross Infection / Q000382:microbiology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field; D007875:Legionella / Q000235:genetics / Q000302:isolation & purification*; D018410:Pneumonia, Bacterial / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial