title: Analysis of the lipid content and the motility of human sperm after follicular fluid treatment.
abstract: The effect of human follicular fluid (hFF) on the cholesterol and phospholipid content and the movement characteristics of human spermatozoa were studied. Semen was selected by a discontinuous Percoll gradient and incubated during in vitro capacitating conditions with B2 medium supplemented with hFF 20%. Percoll pelleted spermatozoa were incubated in either B2 (B2-Percoll) or B2 supplemented with hFF (hFF-Percoll). In hFF-Percoll, we observed a time-dependent (24 h) decrease in both the cholesterol and phospholipid contents (cholesterol: 10.1 vs. 8.7 nmol 10(-7) spermatozoa; phospholipids: 17.5 vs. 15.7 nmol 10(-7) spermatozoa, P < 0.05). This decrease in cholesterol and phospholipids in human spermatozoa was concomitant with a high straight line velocity, a high progressive motility percentage and an increased value of lateral head displacement without any significant alteration of the spermatozoal membrane. No modification of the cholesterol: phospholipid ratio after 2 and 24 h of incubation in either B2-Percoll (0.61, 0.54) in hFF-Percoll (0.59, 0.63) was observed when compared with original control semen. It is suggested that the decrease in cholesterol and phospholipids in hFF-Percoll may be taken into account for the changes of membrane modification as part of the capacitation process.
authors.affiliation: Departement Gynécologie-Obstetrique, INSERM No 316, CHU Bretonneau, Tours, France.
authors.forename: S
authors.initials: S
authors.lastname: Hamamah
authors.forename: M
authors.initials: M
authors.lastname: Lanson
authors.forename: C
authors.initials: C
authors.lastname: Barthelemy
authors.forename: M A
authors.initials: MA
authors.lastname: Garrigue
authors.forename: J P
authors.initials: JP
authors.lastname: Muh
authors.forename: D
authors.initials: D
authors.lastname: Royere
authors.forename: J
authors.initials: J
authors.lastname: Lansac
mesh_terms: D002784:Cholesterol / Q000032:analysis*; D005260:Female; D015571:Follicular Fluid; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010743:Phospholipids / Q000032:analysis*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D010743:Phospholipids; D002784:Cholesterol