Primary toxic effects of anthraquinone-2-sulfonic acid in rat liver microsomes.
(1) The endogenous, NADPH-supported production of H2O2 and of O2-.-radicals in rat liver microsomes was very strongly enhanced in the presence of anthraquinone-2-sulfonic acid (AQSA). (2) This induction of H2O2 and of O2-.-radicals was catalyzed by the microsomal NADPH:cytochrome P450 oxidoreductase (EC 1.6.2.4). (3) AQSA was reduced to AQSA radicals by reductase; AQSA radicals reduce molecular oxygen to O2-.-radicals, which are readily dismutated to H2O2 by the microsomal superoxide dismutase. (4) O2-.-radicals are the sole precursors of all AQSA-induced production of H2O2 in liver microsomes.
Leskovac
V
V
Faculty of Technology, Novi Sad, Yugoslavia.
Trivić
S
S
Peggins
J O
JO
D000818:Animals; D000880:Anthraquinones / Q000378:metabolism / Q000633:toxicity*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004347:Drug Interactions; D006861:Hydrogen Peroxide / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013481:Superoxides / Q000378:metabolism
D000880:Anthraquinones; D013481:Superoxides; C023554:anthraquinone sulfonate; D009249:NADP; D006861:Hydrogen Peroxide; D004121:Dimethyl Sulfoxide