The extracts from rat submandibular glands induce the erythroid differentiation of K-562 cells.
The extracts from rat submandibular glands (SMGs) induced erythroid differentiation of K-562. The activity was non-dialysable and abolished by heat, trypsin or 2-mercaptoethanol. Follistatin, which neutralizes the erythroid differentiation factor (EDF), had no effects on this activity. Analysis by gel chromatography and polyacrylamide gel electrophoresis-isoelectric focussing showed that the characteristics of this substance were different from those of erythropoietin, TGF-beta 1, EDF, stem cell factor and insulin-like growth factor-1. These results suggest the presence of a novel substance in rat SMGs which induces erythroid differentiation of K-562.
Sasaki
R
R
Department of Internal Medicine, Jichi Medical School, Tochigi, Japan.
Masuyama
A
A
Etoh
Y
Y
Yoshida
H
H
Miura
Y
Y
Sasaki
H
H
D000818:Animals; D001560:Benzidines; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006454:Hemoglobins / Q000032:analysis / Q000096:biosynthesis; D006801:Humans; D007525:Isoelectric Focusing; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017208:Rats, Wistar; D013363:Submandibular Gland / Q000737:chemistry* / Q000378:metabolism; D014020:Tissue Extracts / Q000032:analysis / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D001560:Benzidines; D016207:Cytokines; D006454:Hemoglobins; D014020:Tissue Extracts; C029876:benzidine