title: The isolation and sequence of canine interleukin-2.
abstract: A cDNA corresponding to canine IL-2 has been isolated and sequenced. The cDNA was synthesised using RT-PCR, with oligonucleotide primers designed from conserved regions of published IL-2 sequences. The cDNA encodes a predicted full length IL-2 protein of 155 amino-acids. At the nucleic acid level, the canine cDNA shows 92, 88, 88, 82 and 74% homology to published sequences of feline, human, equine, bovine and murine IL-2, respectively. The derived protein shows 90, 86, 85, 76 and 75% similarity to feline, human, equine, murine and bovine IL-2 homologs.
authors.affiliation: Department of Veterinary Pathology, University of Glasgow Veterinary School, UK.
authors.forename: S P
authors.initials: SP
authors.lastname: Dunham
authors.forename: D J
authors.initials: DJ
authors.lastname: Argyle
authors.forename: D E
authors.initials: DE
authors.lastname: Onions
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004285:Dogs / Q000235:genetics*; D006736:Horses; D006801:Humans; D007376:Interleukin-2 / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D007376:Interleukin-2