Emodin inhibits the mutagenicity and DNA adducts induced by 1-nitropyrene.
Polygonum cuspidatum S. (PC) is frequently used as a laxative and an anticancer drug in Chinese medicine. The inhibitory effect of this herb and its component, emodin, on the direct-acting mutagenicity of 1-nitropyrene (1-NP) was examined using the Ames/microsomal test with Salmonella typhimurium TA98 and the genotoxicity of 1-NP was evaluated using the SOS chromotest with E. coli PQ37. Emodin and water extracts of PC markedly decreased the mutagenicity of 1-NP in a dose-dependent manner in both assay systems. Furthermore, emodin and the extracts of PC significantly inhibited the formation of 1-NP DNA adducts in S. typhimurium TA98 in the 32P-postlabeling study. The results suggest that PC extracts and emodin act as blocking and/or suppressing agents to reduce the direct-acting mutagenicity of 1-NP.
Su
H Y
HY
Environmental Toxicological Center, Chung Shan Medical and Dental College, Taichung, Taiwan, ROC.
Cherng
S H
SH
Chen
C C
CC
Lee
H
H
D016587:Antimutagenic Agents / Q000494:pharmacology*; D018736:DNA Adducts; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004642:Emodin / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D009152:Mutagenicity Tests; D009601:Nitroreductases / Q000037:antagonists & inhibitors; D010946:Plants, Medicinal / Q000737:chemistry*; D011721:Pyrenes / Q000494:pharmacology*; D013014:SOS Response, Genetics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000201:enzymology / Q000235:genetics
D016587:Antimutagenic Agents; D018736:DNA Adducts; D004365:Drugs, Chinese Herbal; D011721:Pyrenes; D009601:Nitroreductases; D004642:Emodin; C032668:1-nitropyrene