title: A novel 6Fe (2 x [3Fe-4S]) ferredoxin from Mycobacterium smegmatis.
abstract: A novel ferredoxin was purified from Mycobacterium smegmatis by a series of hydrophobic chromatographies in the presence of high concentrations of ammonium sulfate and sodium chloride. The ferredoxin exhibited the same peptide map and N-terminal amino acid sequence as the known 7Fe ferredoxin from the same bacterium. On the other hand, this ferredoxin was found to contain approximately 6 Fe/mol ferredoxin and was also shown to contain only [3Fe-4S] clusters by resonance Raman spectroscopy, indicating that it is a novel 6Fe ferredoxin which contains two [3Fe-4S] clusters.
authors.affiliation: Department of Chemistry, Rikkyo (St. Paul's) University, Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Imai
authors.forename: A
authors.initials: A
authors.lastname: Urushiyama
authors.forename: H
authors.initials: H
authors.lastname: Saito
authors.forename: Y
authors.initials: Y
authors.lastname: Sakamoto
authors.forename: K
authors.initials: K
authors.lastname: Ota
authors.forename: D
authors.initials: D
authors.lastname: Ohmori
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D005288:Ferredoxins / Q000737:chemistry / Q000302:isolation & purification*; D009161:Mycobacterium / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
chemical_list: D005288:Ferredoxins