title: Double-repetitive-element PCR method for subtyping Mycobacterium tuberculosis clinical isolates.
abstract: We describe a rapid method for subtyping Mycobacterium tuberculosis based on PCR amplification of segments located between two distinct DNA repetitive elements. This method, double-repetitive-element PCR, classified 46 clinical isolates as having 25 distinct patterns; the conventional restriction fragment length polymorphism analysis classified the same isolates as having 23 distinct patterns. The double-repetitive-element PCR is a rapid subtyping method that has a discriminating power similar to that of the restriction fragment length polymorphism method.
authors.affiliation: Division of Infectious Diseases, Cornell University Medical College, New York, New York 10021, USA.
authors.forename: C R
authors.initials: CR
authors.lastname: Friedman
authors.forename: M Y
authors.initials: MY
authors.lastname: Stoeckle
authors.forename: W D
authors.initials: WD
authors.lastname: Johnson
authors.forename: L W
authors.initials: LW
authors.lastname: Riley
mesh_terms: D015373:Bacterial Typing Techniques; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial