title: Genetic and antigenic properties of Dobrava virus: a unique member of the Hantavirus genus, family Bunyaviridae.
abstract: We examined the genetic and antigenic properties of Dobrava (DOB) virus, a hantavirus associated with severe haemorrhagic fever with renal syndrome in Europe. Cloning and sequence analyses revealed the DOB M segment to consist of 3644 nucleotides, with a coding capacity of 1134 amino acids in the virus complementary-sense RNA (cRNA). Seven potential asparagine-linked glycosylation sites were identified in the M segment gene product, one in the G2 and six in the G1 coding regions. The S segment is 1667 nucleotides long, and has a single ORF in the cRNA capable of encoding a protein of 428 amino acids. Phylogenetic comparisons of the M and S segments of DOB virus to those of other hantaviruses indicated that DOB virus is similar to, but clearly distinct from Hantaan (HTN) and Seoul (SEO) viruses. Certain G2-specific, but not G1-specific monoclonal antibodies to HTN virus reacted to the same titre with DOB and homologous viral antigen. Plaque-reduction neutralization tests indicated that, of the sera tested, only antisera to SEO virus were able to neutralize DOB virus to a titre greater than 1:10; however, this neutralization titre was eightfold lower than that observed with homologous SEO virus. The data reported here confirm that DOB virus is a unique species in the Hantavirus genus, family Bunyaviridae.
authors.affiliation: Virology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011, USA.
authors.forename: T
authors.initials: T
authors.lastname: Avsic-Zupanc
authors.forename: A
authors.initials: A
authors.lastname: Toney
authors.forename: K
authors.initials: K
authors.lastname: Anderson
authors.forename: Y K
authors.initials: YK
authors.lastname: Chu
authors.forename: C
authors.initials: C
authors.lastname: Schmaljohn
mesh_terms: D000818:Animals; D001483:Base Sequence; D002522:Chlorocebus aethiops; D017931:DNA Primers; D018076:DNA, Complementary / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D016679:Genome, Viral; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics; D006031:Glycosylation; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D014709:Vero Cells; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D006023:Glycoproteins; D014764:Viral Proteins