Large-scale protoplast isolation and regeneration of Arabidopsis thaliana.
A simple method for large-scale isolation and regeneration of Arabidopsis thaliana green protoplasts utilizing a novel starting material is presented. This technique takes advantage of shooty/embryogenic masses regenerating from fresh or cultured roots. There is no time-consuming mechanical isolation of starting material. Economical Bacto agar is used as an embedding agent. Plating efficiencies are between 5% and 10%. Seventy-six percent of protoplast-derived colonies produce shoots.
Wenck
A R
AR
University of South Carolina, Columbia 29201, USA.
Márton
L
L
D000362:Agar; D017360:Arabidopsis / Q000502:physiology*; D002118:Calcium / Q000494:pharmacology; D002478:Cells, Cultured / Q000166:cytology / Q000187:drug effects; D003080:Cold Temperature; D003470:Culture Media; D011523:Protoplasts / Q000166:cytology / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*
D003470:Culture Media; D000362:Agar; D002118:Calcium