title: Prevalence of anti-endothelial cell antibodies in patients with Sneddon's syndrome.
abstract: BACKGROUND
  Sneddon's syndrome consists of widespread livedo reticularis and ischemic cerebral manifestations. Its pathogenesis remains unclear. Endothelial cells could be the primary target tissue.
  
  
  OBJECTIVE
  Our aim was to determine the prevalence of anti-endothelial cell antibodies (AECA) in a large series of patients with Sneddon's syndrome. The results were compared with those of three groups of control subjects: 39 patients with active periarteritis nodosa, 20 patients hospitalized for stroke without livedo, and 28 healthy persons.
  
  
  METHODS
  AECA were detected with enzyme-linked immunosorbent assay with hybrid cells (EA.hy926) before and after absorption on epithelial cells (A 549/8) to avoid false positivity from antibodies reacting with membranous epithelial antigens.
  
  
  RESULTS
  Twenty-two patients with Sneddon's syndrome had AECA (35%). Of the control subjects, 11 patients with active periarteritis nodosa (28%), 1 of 20 patients with a recent stroke without livedo, and no healthy persons had AECA.
  
  
  CONCLUSIONS
  AECA were frequently found in patients with Sneddon's syndrome, in contrast to the patients with stroke without livedo. The clinical significance and involvement of these antibodies in the pathogenesis of endothelial lesions in Sneddon's syndrome remain to be ascertained.
authors.affiliation: Service de Médecine Interne, Groupe Hospitalier Pitié-Salpêtrière, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Francès
authors.forename: M
authors.initials: M
authors.lastname: Le Tonquèze
authors.forename: K V
authors.initials: KV
authors.lastname: Salohzin
authors.forename: L A
authors.initials: LA
authors.lastname: Kalashnikova
authors.forename: J C
authors.initials: JC
authors.lastname: Piette
authors.forename: P
authors.initials: P
authors.lastname: Godeau
authors.forename: E L
authors.initials: EL
authors.lastname: Nasonov
authors.forename: P
authors.initials: P
authors.lastname: Youinou
mesh_terms: D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000097:blood*; D002545:Brain Ischemia / Q000276:immunology*; D016022:Case-Control Studies; D004727:Endothelium / Q000166:cytology* / Q000276:immunology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012867:Skin / Q000098:blood supply*; D017445:Skin Diseases, Vascular / Q000276:immunology*; D013577:Syndrome
chemical_list: D000906:Antibodies