Isolation of the rat F1-ATPase inhibitor gene and its pseudogenes.
Multiple mitochondrial ATPase inhibitor genes have been identified in the rat-genome. The sequences of two genomic clones indicate that one encodes the functional gene, and the other is a processed pseudogene. The ATPase inhibitor gene isolated is about 1.5 kb long and the coding region contains three exons and two introns. The presence of multiple pseudogenes in the rat is suggested by this study and this is unique since in the bovine genome only a single gene has been found, which is also confirmed here. The presence of multiple inhibitor transcripts in the rat suggests that the functional gene might have multiple transcriptional start sites.
Samuel
D S
DS
Department of Biology, Syracuse University, NY 13244, USA.
Belote
J M
JM
Chan
S H
SH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D004791:Enzyme Inhibitors / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors*; D011544:Pseudogenes / Q000235:genetics*; D051381:Rats
D018076:DNA, Complementary; D004791:Enzyme Inhibitors; D006180:Proton-Translocating ATPases