Importance of the matrix and its chemical activation on the stability of immuno-adsorbents.
Various matrices were reacted with four different activation reagents, in order to prepare immuno-adsorbents for the selective removal of low-density lipoproteins from blood plasma of patients affected by hypercholesterolaemia. The resulting immuno-adsorbents were compared to that obtained earlier with cyanogen bromide activation, in terms of coupling yield, adsorption capacity and moreover stability towards the various chemical and biochemical conditions to which they are submitted during their handling. Tresyl chloride-activated Sepharose 6 Fast Flow turns out to afford optimal stability in the whole pH range tested.
Ubrich
N
N
Centre de recherche INSERM, CHU Brabois, France.
Hubert
P
P
Dellacherie
E
E
Rivat
C
C
D002846:Chromatography, Affinity / Q000379:methods*; D003488:Cyanogen Bromide; D004355:Drug Stability; D006801:Humans; D006937:Hypercholesterolemia / Q000097:blood*; D007164:Immunosorbents; D007202:Indicators and Reagents; D008077:Lipoproteins, LDL / Q000097:blood* / Q000302:isolation & purification; D012685:Sepharose / Q000031:analogs & derivatives
D007164:Immunosorbents; D007202:Indicators and Reagents; D008077:Lipoproteins, LDL; C035771:Sepharose CL 4B; D012685:Sepharose; D003488:Cyanogen Bromide