RK2, a glial-specific homeodomain protein required for embryonic nerve cord condensation and viability in Drosophila.
We report the identification of RK2, a glial-specific homeodomain protein. RK2 is localized to the nucleus of virtually all embryonic and imaginal glial cells, with the exception of midline glia. Embryos mutant for the gene encoding RK2 are embryonic lethal but normal for early gliogenesis (birth, initial divisions and migration of glia) and axonogenesis (neuronal pathfinding and fasciculation). However, later in development, there are significantly fewer longitudinal glia that are spatially disorganized; in addition, there is a slight disorganization of axon fascicles and a defective nerve cord condensation. This suggests that RK2 is not required for early glial determination, but rather for aspects of glial differentiation or function that are required for embryonic viability.
Campbell
G
G
Center for Neurobiology and Behavior, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA.
Göring
H
H
Lin
T
T
Spana
E
E
Andersson
S
S
Doe
C Q
CQ
Tomlinson
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004330:Drosophila / Q000196:embryology*; D029721:Drosophila Proteins; D017344:Genes, Insect; D018398:Homeodomain Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009457:Neuroglia / Q000502:physiology*; D010641:Phenotype
D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; C089972:repo protein, Drosophila