Cloning and characterization of the gene encoding the DsaV methyltransferase.
A gene encoding the M.DsaV methyltransferase was cloned and characterized. The enzyme methylates the internal cytosines in the 5'-CCTGG recognition sequence, as determined by a novel rapid method employing 3H label and exonuclease III.
Gopal
J
J
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
Yebra
M J
MJ
Bhagwat
A S
AS
D001419:Bacteria / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D015257:DNA-Cytosine Methylases / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D015723:Gene Library; D005798:Genes, Bacterial; D016366:Open Reading Frames; D013379:Substrate Specificity
C090447:DNA modification methylase DsaV; D015257:DNA-Cytosine Methylases