Regulation of IgE production from human mononuclear cells by beta 2-adrenoceptor agonists.
The present study examined the effect of beta 2-adrenoceptor agonists on the interleukin-4 (IL-4)-driven IgE production and on the possible mechanisms of action of these compounds. We present evidence that salbutamol and fenoterol potentiated the IL-4-induced IgE production by human peripheral blood mononuclear cells (PBMC). No significant effect of incubation in the presence of beta 2-adrenoceptor agonists on IgG, IgA and IgM production was observed. Salbutamol and fenoterol inhibited interferon-(IFN)-gamma production by PHA-activated human PBMC suggesting that the blockade of the production of this cytokine could possibly explain the enhancement of IgE production. Salbutamol and fenoterol potentiated the IL-4-induced production of sCD23 whereas no effect on CD23 expression was observed. The potentiating effect of salbutamol on IgE production was blocked by two antagonists of beta 2-adrenoceptor, namely butoxamine and D,L-propranolol, suggesting a beta-adrenoceptor-mediated event. These results demonstrate that beta 2-adrenoceptor stimulation results in an increase in IgE production by human B lymphocytes.
Coqueret
O
O
Institut Henri Beaufour, Immunology Department, Les Ulis, France.
Dugas
B
B
Mencia-Huerta
J M
JM
Braquet
P
P
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000420:Albuterol / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007136:Immunoglobulins / Q000097:blood; D015847:Interleukin-4 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008222:Lymphokines / Q000097:blood; D009000:Monocytes / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000502:physiology; D017455:Receptors, IgE / Q000378:metabolism; D012995:Solubility
D000318:Adrenergic beta-Agonists; D007136:Immunoglobulins; D008222:Lymphokines; D011943:Receptors, Adrenergic, beta; D017455:Receptors, IgE; D015847:Interleukin-4; D007073:Immunoglobulin E; D000420:Albuterol