Expression of the ret proto-oncogene in phaeochromocytoma. An in situ hybridization and northern blot study.
The expression and distribution of ret proto-oncogene mRNA were investigated in five phaeochromocytomas of both familial and sporadic types by in situ hybridization (ISH) using digoxigenin-labelled cRNA probes and Northern blot (NB) analysis with random priming labelled cDNA probes. The probes corresponded to the tyrosine kinase domain of the gene. An excellent correlation was found between the ISH and NB results. By both techniques, the expression of the ret proto-oncogene was detected in three of the five cases, two of four familial tumours, and the only sporadic tumour that was studied. The results confirm that ret is frequently expressed in phaeochromocytomas and suggest that it might be an important event in their development and/or progression.
Matias-Guiu
X
X
Department of Pathology, Hospital Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Spain.
Colomer
A
A
Mato
E
E
Cuatrecasas
M
M
Komminoth
P
P
Prat
J
J
Wolfe
H
H
D000310:Adrenal Gland Neoplasms / Q000235:genetics*; D000328:Adult; D015152:Blotting, Northern; D029721:Drosophila Proteins; D005260:Female; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D008875:Middle Aged; D009377:Multiple Endocrine Neoplasia / Q000235:genetics*; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D010673:Pheochromocytoma / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D051096:Proto-Oncogene Proteins c-ret; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis / Q000235:genetics*
D029721:Drosophila Proteins; C000715470:MAS1 protein, human; D009363:Neoplasm Proteins; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D051096:Proto-Oncogene Proteins c-ret; D020794:Receptor Protein-Tyrosine Kinases; C416649:Ret protein, Drosophila