Antioxidant activity of thiocholesterol on copper-induced oxidation of low-density lipoprotein.
The effect of thiocholesterol (SH-Chol) on the copper-induced in vitro oxidation of low-density lipoprotein (LDL; 1.019 < d < 1.063) was investigated. Among the antioxidants tested, including cysteine, glutathione, 2-mercaptoethanol, dithiothreitol, probucol, thiopalmitic acid, and SH-Chol, SH-Chol was the most effective antioxidant in copper-induced LDL oxidation. Also, SH-Chol completely inhibited the formation of oxysterols, i.e., 7-hydroxycholesterol and 7-ketocholesterol, in LDL particles and reduced 1,1-diphenyl-2-picrylhydrazyl used as stable free-radical model. Moreover, SH-Chol suppressed the degradation of endogenous alpha-tocopherol in LDL particles. These findings indicate that SH-Chol acts as antioxidant in the oxidative damage of LDL in vitro and as a free-radical scavenger in lipid peroxidation.
Tanaka
M
M
Showa College of Pharmaceutical Sciences, Tokyo, Japan.
Nakagawa
M
M
D000975:Antioxidants / Q000494:pharmacology*; D002384:Catalysis; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000494:pharmacology; D003300:Copper / Q000037:antagonists & inhibitors*; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D012995:Solubility; D014867:Water / Q000737:chemistry
D000975:Antioxidants; D008077:Lipoproteins, LDL; D014867:Water; C002297:thiocholesterol; D003300:Copper; D002784:Cholesterol