Enhanced intracellular calcium concentration during poliovirus infection.
The infection of human fibroblasts by poliovirus leads to a notable increase in the intracellular calcium concentration, [Ca2+]i, measured by microfluorimetry or by flow cytometry. [Ca2+]i increases from 2 to 3 h postinfection, and by the fifth hour there is a 5- to 10-fold increase in [Ca2+]i. At this time postinfection there is active viral protein synthesis. The modifications in [Ca2+]i are not observed in the presence of cycloheximide, guanidine, or Ro 09-0179, indicating that virus gene expression is required for the increase in [Ca2+]i. Attempts to identify the source of the intracellular Ca2+ by using different inhibitors of calcium fluxes suggest that calcium enters from the culture medium through voltage-sensitive calcium channels.
Irurzun
A
A
Centro de Biología Molecular, CSIC-UAM, Universidad Autónoma de Madrid, Canto Blanco, Spain.
Arroyo
J
J
Alvarez
A
A
Carrasco
L
L
D002118:Calcium / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D005814:Genes, Viral; D006367:HeLa Cells; D006801:Humans; D017955:Poliovirus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D014700:Verapamil / Q000494:pharmacology
D014700:Verapamil; D002118:Calcium