Genomic sequencing indicates a correlation between DNA hypomethylation in the 5' region of the pS2 gene and its expression in human breast cancer cell lines.
The methylation status of the cytosines located on the 5' region of the pS2 gene have been investigated in two human breast cancer cell lines. The genomic sequencing method used is based on an oxydative deamination by NaHSO3 of the unmethylated cytosines, but not 5-methylcytosine. Data obtained indicate that in the DNA extracted from the pS2-expressing cell line, MCF7, the CpG located at the 5' flanking sequence of pS2 are unmethylated. In contrast in the non-expressing cell line, BT20, these CpG are largely methylated. However, this correlation is not observed at all CpG sites, since a significant portion of the cloned PCR fragments obtained from BT20 cells are unmethylated at specific CpG sites. These results suggest that the methylation status of only some of the CpG located at the 5' flanking sequences of pS2 might differ between expressing and nonexpressing cell lines.
Martin
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INSERM U 218, Centre Léon Bérard, Lyon, France.
Ribieras
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Song-Wang
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Rio
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MC
Dante
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D001483:Base Sequence; D001943:Breast Neoplasms; D002460:Cell Line; D002999:Clone Cells; D003001:Cloning, Molecular; D004273:DNA, Neoplasm / Q000235:genetics* / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011506:Proteins; D011994:Recombinant Proteins / Q000096:biosynthesis; D000071163:Trefoil Factor-1; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins
D004273:DNA, Neoplasm; D004967:Estrogens; D009363:Neoplasm Proteins; D011506:Proteins; D011994:Recombinant Proteins; C051838:TFF1 protein, human; D000071163:Trefoil Factor-1; D025521:Tumor Suppressor Proteins