title: Antibodies to the nonstructural protein of parvovirus B19 in persistently infected patients: implications for pathogenesis.
abstract: Three patients with persistent parvovirus B19 infection, as documented by the prolonged presence of IgM directed to the viral capsid proteins and detection of viral DNA in serum by dot-blot hybridization or polymerase chain reaction (PCR), were investigated for the presence of antibodies to the nonstructural protein NS-1 of parvovirus B19. This was done by using an ELISA based on recombinant NS-1 protein. Whereas control sera displayed no reactivity, sera from persistently infected patients showed a strong specific antibody response to NS-1. Patients were followed for 3-18 months, during which IgM titers declined but IgG directed to the nonstructural protein remained detectable. The appearance of NS-1-specific antibodies might indicate an altered course of viral infection leading to the establishment of persistently active infection and subsequent destruction of cells of nonerythroid lineage.
authors.affiliation: Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: von Poblotzki
authors.forename: A
authors.initials: A
authors.lastname: Hemauer
authors.forename: A
authors.initials: A
authors.lastname: Gigler
authors.forename: E
authors.initials: E
authors.lastname: Puchhammer-Stöckl
authors.forename: F X
authors.initials: FX
authors.lastname: Heinz
authors.forename: J
authors.initials: J
authors.lastname: Pont
authors.forename: K
authors.initials: K
authors.lastname: Laczika
authors.forename: H
authors.initials: H
authors.lastname: Wolf
authors.forename: S
authors.initials: S
authors.lastname: Modrow
mesh_terms: D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000097:blood*; D000917:Antibody Formation; D004279:DNA, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D016731:Erythema Infectiosum / Q000276:immunology*; D005260:Female; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D008875:Middle Aged; D016732:Parvovirus B19, Human / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D011994:Recombinant Proteins / Q000276:immunology; D013997:Time Factors; D017361:Viral Nonstructural Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D007075:Immunoglobulin M; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins