title: Adrenomedullin inhibits the secretion of cytokine-induced neutrophil chemoattractant, a member of the interleukin-8 family, from rat alveolar macrophages.
abstract: This study was undertaken to determine the effects of adrenomedullin (AM) on the secretion of cytokine-induced neutrophil chemoattractant (CINC), a member of the interleukin-8 family, from lipopolysaccharide-stimulated rat alveolar macrophages in vitro. AM significantly increased cAMP levels in alveolar macrophages in a dose-dependent fashion. On the other hand, AM significantly inhibited CINC secretion from alveolar macrophages in a dose-dependent fashion, and 8-bromo-cyclic adenosine monophosphate (8-Br-cAMP) also significantly inhibited CINC secretion. These findings suggest that AM may play important roles in the regulation of airway inflammation via a cAMP-dependent mechanism.
authors.affiliation: Second Department of Biochemistry, Osaka City University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kamoi
authors.forename: H
authors.initials: H
authors.lastname: Kanazawa
authors.forename: K
authors.initials: K
authors.lastname: Hirata
authors.forename: N
authors.initials: N
authors.lastname: Kurihara
authors.forename: Y
authors.initials: Y
authors.lastname: Yano
authors.forename: S
authors.initials: S
authors.lastname: Otani
mesh_terms: D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D053607:Adrenomedullin; D000818:Animals; D001993:Bronchodilator Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000145:classification / Q000378:metabolism*; D000242:Cyclic AMP / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D006133:Growth Substances / Q000145:classification / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8 / Q000145:classification; D008070:Lipopolysaccharides / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000187:drug effects*; D008297:Male; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001993:Bronchodilator Agents; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D008070:Lipopolysaccharides; D010455:Peptides; D053607:Adrenomedullin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP