title: Preconditioning with dobutamine in the isolated rat heart.
abstract: The ability of dobutamine to precondition the isolated rat heart against postischemic contractile dysfunction was assessed. Hearts were perfused with varying concentrations of dobutamine for 5 min followed by a 5 min "washout" period and 30 min of global ischemia. The hearts were reperfused for 30 min to assess postischemic function. Dobutamine improved postischemic developed pressure, +dp/dt, heart rate x developed pressure, end diastolic pressure, and coronary flow in a concentration-dependent manner. The concentration of dobutamine showing the maximum protective effect was 10(-6)M. Propranolol administered with dobutamine significantly attenuated the protective effect. The results indicate that transient treatment with dobutamine can precondition the rat heart against ischemia/reperfusion injury. The mechanism of protection appears to involve beta-adrenergic stimulation.
authors.affiliation: Department of Surgery, University of Texas Medical Branch, Galveston 77555-0528, USA.
authors.forename: G K
authors.initials: GK
authors.lastname: Asimakis
authors.forename: V R
authors.initials: VR
authors.lastname: Conti
mesh_terms: D000818:Animals; D004280:Dobutamine / Q000037:antagonists & inhibitors / Q000627:therapeutic use*; D004347:Drug Interactions; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D006339:Heart Rate / Q000187:drug effects; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects; D017202:Myocardial Ischemia / Q000209:etiology / Q000517:prevention & control*; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D016277:Ventricular Function, Left / Q000187:drug effects; D017725:Ventricular Pressure / Q000187:drug effects
chemical_list: D007773:Lactates; D019344:Lactic Acid; D004280:Dobutamine; D011433:Propranolol