title: A YAC contig map of Plasmodium falciparum chromosome 4: characterization of a DNA amplification between two recently separated isolates.
abstract: We have generated a physical map of Plasmodium falciparum chromosome 4 using yeast artificial chromosomes (YACs). The map is defined by a YAC contig spanning approximately 1.05 Mb, which has been restriction mapped to a resolution of 30 kb and is punctuated by 22 sequence-tagged sites. The physical information obtained has enabled us to compare and contrast the structure of chromosome 4 in detail between FCR3 and B8, two recently separated isolates of P. falciparum, leading to characterization of a novel chromosome polymorphism occurring in a subtelomeric region. Comparison of chromosomes 4 from 10 different isolates has shown that chromosome size polymorphisms are restricted to both subtelomeric regions. These analyses provide a high-resolution physical map that will be important to complement genetic analysis of this human pathogen.
authors.affiliation: Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Victoria, Australia.
authors.forename: J P
authors.initials: JP
authors.lastname: Rubio
authors.forename: T
authors.initials: T
authors.lastname: Triglia
authors.forename: D J
authors.initials: DJ
authors.lastname: Kemp
authors.forename: D
authors.initials: D
authors.lastname: de Bruin
authors.forename: J V
authors.initials: JV
authors.lastname: Ravetch
authors.forename: A F
authors.initials: AF
authors.lastname: Cowman
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D017125:Genes, Protozoan; D016678:Genome; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D016324:Sequence Tagged Sites