Localization of pro-collagen type II mRNA and collagen type II in the healing tooth socket of the rat.
Sprague-Dawley rats (50 days old) were anaesthetized and the maxillary right molars extracted. The rats were killed at 2, 3, 6, 8 and 10 days after extraction. The maxillae were dissected and prepared for either routine histology, in situ hybridization for pro-collagen type II mRNA, or immunohistochemical detection of collagen type II. Pro-collagen type II mRNA was expressed maximally in the healing tooth socket at 8 days after the extractions, but the protein was not expressed at any time. This suggests that the translation of pro-collagen type II mRNA does not occur in osteoblasts following tooth extraction. Ossification was present in the socket at 6 days after the extractions, which is consistent with the suggestion that an early feature of osteoblastic differentiation may be the expression of type II pro-collagen mRNA.
Devlin
H
H
Department of Restorative Dentistry, University Dental Hospital of Manchester, U.K.
Hoyland
J
J
Freemont
A J
AJ
Sloan
P
P
D000539:Alveolar Process / Q000737:chemistry* / Q000502:physiology; D000818:Animals; D003094:Collagen / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D007150:Immunohistochemistry; D010006:Osteoblasts / Q000378:metabolism; D011347:Procollagen / Q000096:biosynthesis*; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014081:Tooth Extraction; D014945:Wound Healing / Q000502:physiology*
D011347:Procollagen; D012333:RNA, Messenger; D003094:Collagen