Effects of sex steroids on cell growth and C-myc oncogene expression in LN-CaP and DU-145 prostatic carcinoma cell lines.
The prostate gland is dependent on androgens for the maintenance of its normal growth and functional integrity. Initially, growth of the majority of prostate tumours can be manipulated by endocrine therapy. In the present study, we evaluated the effects of sex steroids on the cell growth and expression of the C-myc oncogene in two human prostatic adenocarcinoma cell lines. We found that dihydrotestosterone increases the proliferation rate of prostatic cells, and amplification of C-myc oncogene is hormone-dependent. We also demonstrated a positive correlation between the number of cells positive for C-myc oncogene and oncoprotein in hormone-treated prostate cell lines.
Asadi
F K
FK
Department of Urology, University of Illinois Medical Center at Chicago, USA.
Sharifi
R
R
D000230:Adenocarcinoma / Q000235:genetics*; D015139:Blotting, Southern; D002455:Cell Division / Q000187:drug effects; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D006801:Humans; D017403:In Situ Hybridization; D066298:In Vitro Techniques; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D016271:Proto-Oncogene Proteins c-myc; D013196:Dihydrotestosterone; D004958:Estradiol