[Hypocrellin B-ethanolamine (HB-E) sensitized photodamage on rat mitochondria].
The Hypocrellin B-Ethanolamine (HB-E) sensitized photodamage on lipid peroxidation, sulfhydryl oxidation and ATPase inactivation of rat liver mitochondria was studied under illumination of 640 nm, The HB-E sensitized photodamage of lipid peroxidation is less than sulfhydryl photooxidation, when HB-E concentration is 50 mumol/L. To elucidate the mechanism of photodamage of HB-E, antioxidant: BHT, Vit E, oxygen radical quencher: L-His, beta-Cart, were investigated. The results showed that HB photodamage of mitochondria is not only by oxygen radical, but also by HB-E radical as well. From compared tests among HB, HA, Hp, MB and HB-E, we found that HB-E had higher ability to photodamage lipid peroxidation, sulfhydryl oxidation and tryptophan than other photosensitizers. The experiments showed that the superoxide generated may be by HB-E radical.
Yue
J C
JC
Institute of Biophysics, Academia Sinica, Beijing, China.
Liu
M
M
Wan
P
P
Pang
S Z
SZ
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D004983:Ethanolamines / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism; D017319:Photosensitizing Agents / Q000494:pharmacology*; D011809:Quinones / Q000494:pharmacology*; D051381:Rats; D014364:Tryptophan / Q000378:metabolism
D004983:Ethanolamines; D017319:Photosensitizing Agents; D011809:Quinones; C094309:hypocrellin B ethanolamine; D008315:Malondialdehyde; D014364:Tryptophan; D000251:Adenosine Triphosphatases