title: Integrin alpha 5 during early development of Xenopus laevis.
abstract: The full length sequence of the Xenopus integrin alpha 5 subunit is reported. Analysis of cloned cDNA fragments reveals that alternative polyadenylation of alpha 5 mRNA occurs in the embryo. Furthermore, a variant form of the alpha 5 mRNA is expressed which encodes an integrin alpha 5 subunit with a truncated cytoplasmic domain. Integrin alpha 5 mRNA and protein are expressed in oocytes, eggs and throughout development. Spatial expression of alpha 5 mRNAs is first detected by whole mount in situ hybridization in presumptive neural crest cells and in the somitic mesoderm from the midgastrula stage onwards. In contrast, the alpha 5 protein is present on newly formed plasma membranes beginning at first cleavage. During neurulation, the integrin alpha 5 subunit disappears from the outer layer of the ectoderm, the notochord and the neural tube and accumulates in the sensorial layer of the ectoderm, the somites and the neural crest cells. These results provide evidence for the position specific regulation of alpha subunit expression in early vertebrate embryos.
authors.affiliation: Max-Planck-Institut für Entwicklungsbiologie, Abt. V, Tübingen, FRG.
authors.forename: T O
authors.initials: TO
authors.lastname: Joos
authors.forename: C A
authors.initials: CA
authors.lastname: Whittaker
authors.forename: F
authors.initials: F
authors.lastname: Meng
authors.forename: D W
authors.initials: DW
authors.lastname: DeSimone
authors.forename: V
authors.initials: V
authors.lastname: Gnau
authors.forename: P
authors.initials: P
authors.lastname: Hausen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D002970:Cleavage Stage, Ovum; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D014644:Genetic Variation; D039482:Integrin alpha5; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D014982:Xenopus laevis
chemical_list: D015703:Antigens, CD; D039482:Integrin alpha5; D012333:RNA, Messenger