Trypanosoma cruzi immunosuppressive factor decreases the interleukin-2 mRNA level in cultured normal activated human lymphocytes.
We show here that the marked inhibition of interleukin-2 production seen in hosts acutely infected with Trypanosoma cruzi can be reproduced in vitro by adding a T. cruzi suspension filtrate to cultures of activated human peripheral blood mononuclear cells. This effect was not due to an accelerated decay of interleukin-2 mRNA and appeared to be selective since the levels of other mRNA molecules relevant to lymphocyte activation were not noticeably decreased. The use of normal lymphoid cells in this in vitro system makes it possible to examine the mechanisms used by T. cruzi to induce abnormalities in lymphocyte functions that also occur in infected hosts.
Majumder
S
S
Department of Microbiology, Michigan State University, East Lansing 48824, USA.
Kierszenbaum
F
F
D000818:Animals; D002478:Cells, Cultured; D014355:Chagas Disease / Q000276:immunology*; D005786:Gene Expression Regulation; D006801:Humans; D007108:Immune Tolerance; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007376:Interleukin-2 / Q000235:genetics*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D015800:Protozoan Proteins / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000276:immunology*
D007166:Immunosuppressive Agents; D007376:Interleukin-2; D015800:Protozoan Proteins; D012333:RNA, Messenger