title: Point mutation screening for 16 exons of the dystrophin gene by multiplex single-strand conformation polymorphism analysis.
abstract: We have developed a rapid and nonradioactive method to screen for point mutations using the Pharmacia PhastSystem. In an SSCP analysis, we applied the two multiplex exon PCR kits, commonly used for the detection of deletions in Duchenne and Becker muscular dystrophy patients. The different exon bands in the multiplex SSCP pattern could be identified by running well-characterised deletion patients in this system. Two common polymorphisms were easily identifiable and are helpful in the haplotype analysis in families. Screening of 70 patients in which no gross rearrangement was detectable with the multiplex PCR and Southern blot, resulted in the identification of 6 patients with a band shift after SSCP analysis. Of these 6 band shifts, 5 were the result of a frame shift or termination mutation. The other band shift was found to be a rare polymorphism unlikely to be the cause of the patient's phenotype. Application of this technique enabled us to improve diagnosis in the families involved and will allow us to extend the search for point mutations in the remaining exons of the dystrophin gene.
authors.affiliation: MGC Department of Human Genetics, Leiden University, University Hospital Leiden, The Netherlands.
authors.forename: A L
authors.initials: AL
authors.lastname: Kneppers
authors.forename: P P
authors.initials: PP
authors.lastname: Deutz-Terlouw
authors.forename: J T
authors.initials: JT
authors.lastname: den Dunnen
authors.forename: G J
authors.initials: GJ
authors.lastname: van Ommen
authors.forename: E
authors.initials: E
authors.lastname: Bakker
mesh_terms: D015139:Blotting, Southern; D016189:Dystrophin / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005091:Exons; D005260:Female; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D008297:Male; D009136:Muscular Dystrophies / Q000235:genetics*; D010375:Pedigree; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA
chemical_list: D016189:Dystrophin