Allergen-specific human T cell clones produce interleukin-5 upon stimulation with the Th1 cytokine interleukin-2.
CD4+ T cell clones specific for Der fII (a major allergen of the house dust mite) were established from peripheral blood mononuclear cells of atopic patients. All of the T cell clones were classified as having the Th0 phenotype, since they produced both interleukin (IL)-2 and IL-4 upon stimulation. Some of the clones produced IL-5 upon antigenic stimulation. Human recombinant IL-2 induced these T cell clones to express IL-5 mRNA and produce IL-5 protein in a dose-dependent manner. IL-2 did not induce IL-4 production, indicating a discrete signal requirement for IL-4 versus IL-5 production by T cells. Moreover, IL-5 production induced by immobilized anti-CD3 monoclonal antibody was completely suppressed by the addition of anti-IL-2 monoclonal antibody, suggesting that IL-5 production, designated as a Th2-type immune response, is dependent on IL-2, a Thl cytokine. IL-2 produced at the site of allergic inflammation may contribute to IL-5 production by T cells in vivo.
Mori
A
A
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Japan.
Suko
M
M
Tsuruoka
N
N
Kaminuma
O
O
Ohmura
T
T
Nishizaki
Y
Y
Ito
K
K
Okudaira
H
H
D000485:Allergens / Q000276:immunology*; D000818:Animals; D039741:Antigens, Dermatophagoides; D001249:Asthma / Q000097:blood / Q000276:immunology / Q000473:pathology; D002478:Cells, Cultured; D002999:Clone Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D015848:Interleukin-5 / Q000096:biosynthesis* / Q000235:genetics; D008925:Mites / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
D000485:Allergens; D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D007376:Interleukin-2; D015848:Interleukin-5; D011994:Recombinant Proteins