Intestinal beta-carotene absorption and cleavage in men: response of beta-carotene and retinyl esters in the triglyceride-rich lipoprotein fraction after a single oral dose of beta-carotene.
Postprandial response curves of beta-carotene and retinyl esters in a triglyceride-rich lipoprotein (TRL) fraction were evaluated as a potential measure of beta-carotene uptake and cleavage. beta-Carotene, retinyl ester, and triglyceride concentrations in the TRL fraction (density < 1.006 kg/L) and plasma were measured in 10 men for 8 or 16 h after an oral dose of 15 mg beta-carotene. The beta-carotene response, unlike the triglyceride and retinyl ester response, can be evaluated in the TRL fraction but not in plasma. Intraindividual variations in the triglyceride-adjusted response of beta-carotene and retinyl palmitate in TRL fractions were 23% and 20% and interindividual variations were 42% and 36%, respectively. A low beta-carotene response was associated with a high ratio between retinyl palmitate and beta-carotene responses (r = -0.56, P = 0.013). In conclusion, the measurement of beta-carotene and retinyl esters in the TRL fraction after a dose of beta-carotene with a vitamin A-free meal may be an appropriate method to study beta-carotene uptake and cleavage.
van Vliet
T
T
TNO Nutrition and Food Research Institute, Zeist, Netherlands.
Schreurs
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WH
van den Berg
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D000284:Administration, Oral; D000328:Adult; D002338:Carotenoids / Q000008:administration & dosage / Q000378:metabolism* / Q000493:pharmacokinetics*; D004224:Diterpenes; D006801:Humans; D007408:Intestinal Absorption / Q000187:drug effects / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000502:physiology; D008074:Lipoproteins / Q000032:analysis / Q000097:blood*; D008297:Male; D000084562:Retinyl Esters; D014280:Triglycerides / Q000032:analysis / Q000097:blood*; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000032:analysis / Q000097:blood; D019207:beta Carotene
D004224:Diterpenes; D008074:Lipoproteins; D000084562:Retinyl Esters; D014280:Triglycerides; D019207:beta Carotene; D014801:Vitamin A; C014794:retinol palmitate; D002338:Carotenoids