Expression of cytoskeletal and matrix genes following exposure to ionizing radiation: dose-rate effects and protein synthesis requirements.
Experiments were designed to examine the effects of radiation dose-rate and cycloheximide on expression of cytoskeletal and matrix elements in Syrian hamster embryo cells. Results here demonstrated little effect of dose-rate for fission-spectrum neutrons when comparing expression of alpha-tubulin or fibronectin genes. Effects of cycloheximide, however, revealed several findings: (a) Cycloheximide repressed accumulation of alpha-tubulin following exposure to high dose-rate neutrons or gamma-rays. (b) Cycloheximide did not affect accumulation of mRNA for actin genes. (c) Cycloheximide abrogated the moderate induction of fibronectin-mRNA which occurred following exposure to gamma-rays and high dose-rate neutrons. These results suggest a role for labile proteins in the maintenance of alpha-tubulin and fibronectin mRNA accumulation following exposure to ionizing radiation.
Woloschak
G E
GE
Center for Mechanistic Biology and Biotechnology, Argonne National Laboratory, Illinois 60439-4811, USA.
Felcher
P
P
Chang-Liu
C M
CM
D000818:Animals; D002478:Cells, Cultured; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D003598:Cytoskeletal Proteins / Q000096:biosynthesis* / Q000235:genetics*; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005109:Extracellular Matrix / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics*; D005347:Fibroblasts / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D015870:Gene Expression / Q000528:radiation effects; D008647:Mesocricetus; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D003598:Cytoskeletal Proteins; D016326:Extracellular Matrix Proteins; D012333:RNA, Messenger; D003513:Cycloheximide