Influence of Pseudomonas aeruginosa exoproducts on virulence factor production in Burkholderia cepacia: evidence of interspecies communication.
The effect of concentrated cell-free extracellular material from stationary-phase cultures of Burkholderia cepacia 10661 and Pseudomonas aeruginosa PAO1 on virulence factor production in B. cepacia was assessed. While increasing concentrations of the B. cepacia exoproduct caused a slight increase in siderophore, lipase, and protease production in the producing organism, a significant in productivity was observed for all three virulence factors with the addition of the PAO1 exoproduct. Moreover, the addition of the exoproduct from a strain of P. aeruginosa producing reduced amounts of autoinducer caused only a slightly greater response than that of the control. Both B. cepacia 10661 and P. aeruginosa PAO1, along with two matched clinical isolates of both organisms obtained from a cystic fibrotic patient, were shown to produce variable amounts of three different types of autoinducer. The potential for interspecies signalling in microbial pathogenicity is discussed.
McKenney
D
D
Department of Pharmacy, University of Manchester, England, United Kingdom.
Brown
K E
KE
Allison
D G
DG
D016956:Burkholderia cepacia / Q000472:pathogenicity / Q000502:physiology*; D010450:Endopeptidases / Q000378:metabolism; D008049:Lipase / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D017262:Siderophores / Q000378:metabolism; D014774:Virulence
D017262:Siderophores; D008049:Lipase; D010450:Endopeptidases