[Damage effects of methylmercury on cell membrane].
Damage to cell membrane system caused by methylmercury (Me-Hg) and its mechanism were studied with biochemical and cell biological methods experimentally. Results showed erythrocyte membrane and activities of T-ATPase, Mg(++)-ATPase and Na(+)-K(+)-ATPase of microsome membrane in brain, liver and kidney of the exposed animals decreased more significantly than of controls with a dose-effect pattern. Activities of Mg(++)-ATPase and Na(+)-K(+)-ATPase of erythrocyte membrane correlated positively with those of microsome membrane in brain and kidney. Sulfhydryl content of erythrocyte membrane and brain microsome membrane decreased significantly in the exposed animals (P < 0.01). Sulfhydryl content correlated inversely with the activities of above three kinds of ATPase in kidney, but so did in brain positively. Fluorescent polarization of membranes of erythrocytes and microsome in brain, liver and kidney increased, membrane fluidity decreased, LDH activities increased in plasma and decreased in cells and cell permeability increased in the exposed animals. Activities of respiratory enzymes of mitochondrion in exposed animals were lower than those in controls (P < 0.05). 3H-TdR incorporation was inhibited in spleen, liver and brain in exposed animals. Abilities of DNA synthesis and UDS repair were inhibited with high-dose of Me-Hg, and stimulated with low-dose. SCE frequency in bone marrow cells increased with dose of Me-Hg (P < 0.001). Proportion of splenocyte from G0/G1 to S phases increased and those from S to G2/M phases lowered, and it suggested DNA synthesis had been inhibited.
Lin
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X
School of Preventive Medicine, Norman Bethune University of Medical Sciences, Changchun.
Li
Y
Y
Mu
Y
Y
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000201:enzymology; D008767:Methylmercury Compounds / Q000633:toxicity*; D051379:Mice; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D008767:Methylmercury Compounds; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase