title: A cell type-specific silencer in the human choline acetyltransferase gene requiring two distinct and interactive E boxes.
abstract: We have previously reported that cholinergic neuron-specific expression of the human choline acetyltransferase gene is mediated by two co-operative silencers. We have now localized the proximal silencer to the region from nucleotide -2195 to -2409, which contains two distinct E boxes (CACCTG and CATGTG). Deletion or mutation of either of these E boxes results in a loss of silencer activity. There are specific nuclear proteins in adrenergic cells which bind to each of the two E boxes. However, nuclear proteins from cholinergic cells only bind the 5' E box not the 3' E box. It is this interaction which appears to be the cause of the inactivity of this silencer in these cells.
authors.affiliation: Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas 75235, USA.
authors.forename: Y P
authors.initials: YP
authors.lastname: Li
authors.forename: F
authors.initials: F
authors.lastname: Baskin
authors.forename: R
authors.initials: R
authors.lastname: Davis
authors.forename: D
authors.initials: D
authors.lastname: Wu
authors.forename: L B
authors.initials: LB
authors.lastname: Hersh
mesh_terms: D001483:Base Sequence; D002795:Choline O-Acetyltransferase / Q000096:biosynthesis* / Q000235:genetics; D015342:DNA Probes; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D009447:Neuroblastoma / Q000235:genetics; D009474:Neurons / Q000201:enzymology / Q000378:metabolism*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D015342:DNA Probes; D002795:Choline O-Acetyltransferase