Survivability and leucocyte migration inhibition in mice immunized with cryotreated ascites fibrosarcoma cells using different freeze-thaw cycles.
Attempts have been made to assess as to what extent in vitro assay of cellular immunity, e.g. leucocyte migration inhibition (LMI) in mice immunized with different freeze-thaw cycles could reflect host resistance in vivo. While survivability of animals improved significantly by immunization with single cycle (P < 0.05) to three cycle (P < 0.001) and programmed three cycle (P < 0.001) cryo-treated tumor cells compared to controls, the percentage LMI in the same groups of animals decreased progressively. The KCl(3M) extracted tumor cell protein (antigen) of both viable and cryo-treated cells showed a progressively increased protein concentrations per 1 x 10(6) tumor cells with viable cells being least and programmed three cycle cryo-treated cells highest. The apparent discrepancy observed between percentage migration inhibition and survivability may be due to the fact that (1) survivability is a function of body's total immune response while LMI represents the response of one effector limb only; (2) immuno-regulatory mechanisms depend on a balance between activation and suppression and suppressor cells being more sensitive and of shorter life span, affect migration inhibition but not the survivability; (3) cryo-treatment alters tumor cell surface antigen affecting immunological balance; and (4) suppressor and antitumor activities against antigenic stimulation develop simultaneously in different organs and LMI performed with sensitized splenic cells, where, perhaps, suppressor cell activity dominates.
Roy
A
A
Department of Nuclear & Experimental Medical Sciences, Institute of Post Craduate Medical Education & Research, Calcutta, India.
Lahiri
S
S
Lahiri
P
P
Pal
S
S
Ghosh
S
S
Roy
B
B
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D001201:Ascites / Q000276:immunology*; D002470:Cell Survival; D002634:Chemotaxis, Leukocyte; D015925:Cryopreservation; D005354:Fibrosarcoma / Q000276:immunology*; D007111:Immunity, Cellular; D007962:Leukocytes / Q000166:cytology* / Q000276:immunology; D008297:Male; D051379:Mice; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface