Detection of planarian Antennapedia-like homeobox genes expressed during regeneration.
Using the polymerase chain reaction with degenerate oligo primers we identified six new Antennapedia-like homeobox sequences expressed in a regenerating planarian. cDNA fragments containing the entire homeobox sequences for four of these genes were obtained. Two of them, Dutarh-3 and Dutarh-4, belong to the classical Antennapedia type and display extensive identity with Antennapedia- and Deformed-type homeodomains respectively. The third homeodomain, Dutarh-1, exhibits some similarity to the Hox1.5-, AbdB- and Dfd-type homeodomains and a fourth gene, Dutarh-6, is very similar to Mox2 in the mouse. Our results suggest that, in spite of their simple body plan, planarians contain a number of Antennapedia-like homeobox genes, probably enough to fill a cluster such as found in higher animals.
Tarabykin
V S
VS
Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
Lukyanov
K A
KA
Potapov
V K
VK
Lukyanov
S A
SA
D000595:Amino Acid Sequence; D000818:Animals; D051943:Antennapedia Homeodomain Protein; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D017238:Genes, Helminth / Q000235:genetics*; D005801:Genes, Homeobox / Q000235:genetics*; D018398:Homeodomain Proteins; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D010932:Planarians / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012038:Regeneration / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors
D051943:Antennapedia Homeodomain Protein; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors