Analysis of the lipid content and the motility of human sperm after follicular fluid treatment.
The effect of human follicular fluid (hFF) on the cholesterol and phospholipid content and the movement characteristics of human spermatozoa were studied. Semen was selected by a discontinuous Percoll gradient and incubated during in vitro capacitating conditions with B2 medium supplemented with hFF 20%. Percoll pelleted spermatozoa were incubated in either B2 (B2-Percoll) or B2 supplemented with hFF (hFF-Percoll). In hFF-Percoll, we observed a time-dependent (24 h) decrease in both the cholesterol and phospholipid contents (cholesterol: 10.1 vs. 8.7 nmol 10(-7) spermatozoa; phospholipids: 17.5 vs. 15.7 nmol 10(-7) spermatozoa, P < 0.05). This decrease in cholesterol and phospholipids in human spermatozoa was concomitant with a high straight line velocity, a high progressive motility percentage and an increased value of lateral head displacement without any significant alteration of the spermatozoal membrane. No modification of the cholesterol: phospholipid ratio after 2 and 24 h of incubation in either B2-Percoll (0.61, 0.54) in hFF-Percoll (0.59, 0.63) was observed when compared with original control semen. It is suggested that the decrease in cholesterol and phospholipids in hFF-Percoll may be taken into account for the changes of membrane modification as part of the capacitation process.
Hamamah
S
S
Departement Gynécologie-Obstetrique, INSERM No 316, CHU Bretonneau, Tours, France.
Lanson
M
M
Barthelemy
C
C
Garrigue
M A
MA
Muh
J P
JP
Royere
D
D
Lansac
J
J
D002784:Cholesterol / Q000032:analysis*; D005260:Female; D015571:Follicular Fluid; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010743:Phospholipids / Q000032:analysis*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000737:chemistry / Q000187:drug effects* / Q000648:ultrastructure
D010743:Phospholipids; D002784:Cholesterol