The promoting action of magnesium lithospermate B on the kinin-prostaglandin E2 system in the kidney.
The effect of magnesium lithospermate B purified from Salviae Miltiorrhizae Radix on the kinin-prostaglandin E2 system was investigated using kidney slices and isolated kidney microsomes. Magnesium lithospermate B markedly increased the amount of thiobarbituric acid-reactive substances produced by incubation of arachidonic acid in renal slices. Enhancement of arachidonic acid-mediated oxygenation of 1,3-diphenylisobenzofuran was also observed in renal microsomes from rats treated with magnesium lithospermate B, indicating an increase of prostaglandin biosynthesis. Furthermore, magnesium lithospermate B significantly increased the synthesis of prostaglandin E2 in renal slices, but the magnesium lithospermate B-mediated response was blunted with kinin antagonist. On the basis of the above results, it is apparent that magnesium lithospermate B exerts an influence on the prostaglandin system via kinin receptors.
Chung
H Y
HY
College of Pharmacy, Pusan National University, Gum Jung-Ku, Korea.
Kim
J S
JS
Chung
H Y
HY
Yokozawa
T
T
Oura
H
H
D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D001572:Benzofurans / Q000378:metabolism; D001920:Bradykinin / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000494:pharmacology; D015232:Dinoprostone / Q000096:biosynthesis*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018002:Receptors, Bradykinin / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D001572:Benzofurans; D004365:Drugs, Chinese Herbal; D018002:Receptors, Bradykinin; D017392:Thiobarbituric Acid Reactive Substances; C053748:B 4162; C061091:lithospermate B; D016718:Arachidonic Acid; C011238:1,3-diphenylisobenzofuran; D015232:Dinoprostone; D010100:Oxygen; D001920:Bradykinin