Molecular cloning of the mouse osteoglycin-encoding gene.
Osteoglycin (OG) is a glycoprotein that was first isolated from bovine bone. The deduced amino acid (aa) sequence from the cDNA analysis showed that a precursor of OG has consensus leucine-rich repeats. In this study, we have isolated from a mouse limb-bud library cDNA clones encoding a 298-aa OG. This molecule shows 85 and 86% homology to human and bovine OG, respectively. Furthermore, the C-terminal two-thirds of the protein shows 48% homology to the corresponding portion of chick proteoglycan (PG)-Lb, a PG that has been shown to be preferentially expressed in the zone of flattened chondrocytes in the developing limb cartilage. Northern blot analysis of various mouse tissues revealed a 3.7-kb transcript in a limited number of these tissues.
Ujita
M
M
Institute for Molecular Science of Medicine, Aichi Medical University, Japan.
Shinomura
T
T
Kimata
K
K
D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D003871:Dermatan Sulfate / Q000235:genetics; D005121:Extremities / Q000196:embryology; D015698:Genomic Library; D006023:Glycoproteins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D011509:Proteoglycans; D017386:Sequence Homology, Amino Acid; D000071232:Small Leucine-Rich Proteoglycans; D013045:Species Specificity
D030161:Avian Proteins; D011508:Chondroitin Sulfate Proteoglycans; D018076:DNA, Complementary; C504412:Epyc protein, mouse; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C512906:Ogn protein, mouse; D011509:Proteoglycans; D000071232:Small Leucine-Rich Proteoglycans; C072190:DSPG3 protein, chicken; D003871:Dermatan Sulfate