title: Cytotoxigenicity in Salmonella serovars.
abstract: Twenty two strains of Salmonella belonging to eight different serovars, namely S. enterica subsp. enterica serovar S. typhimurium, S. nchanga, S. newport, S. virchow, S. bovismorbificans, S. seftenberg, S. weltevreden and S. indiana, isolated from foods of animal origin, were tested for their cytotoxicity on MDBK and Vero cell-lines. Although all the strains were found to be cytotoxic for both the cell-lines, their cytotoxic activity varied greatly. A dose-related cytotoxic effect was observed. Polymyxin B sulphate @.25 mg/ml in PBS, pH 7.2), urea (8M in Tris-HCl buffer, pH 8.2) and cell-sonication were found to augment the release of cytotoxin.
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary Sciences, G.B. Pant University of Agriculture & Technology, Pantnagar, India.
authors.forename: P
authors.initials: P
authors.lastname: Malik
authors.forename: V D
authors.initials: VD
authors.lastname: Sharma
authors.forename: R
authors.initials: R
authors.lastname: Chandra
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism / Q000633:toxicity; D002417:Cattle; D002470:Cell Survival; D002474:Cell-Free System; D002522:Chlorocebus aethiops; D011112:Polymyxin B / Q000494:pharmacology; D012475:Salmonella / Q000145:classification / Q000472:pathogenicity*; D012703:Serotyping; D013045:Species Specificity; D014508:Urea / Q000494:pharmacology; D014709:Vero Cells
chemical_list: D001427:Bacterial Toxins; D014508:Urea; D011112:Polymyxin B