Canine oligodendrocytes undergo morphological changes in response to basic fibroblast growth factor (bFGF) in vitro.
The mature oligodendrocyte, though able to divide under certain circumstances, has been regarded as incapable of changing into a phenotypically plastic cell type. To further explore this question, we developed an in vitro system in which a virtually pure population of early postnatal canine oligodendrocytes were cultured in a serum free, defined medium. We tested the oligodendrocytes' morphological and mitotic responses to concentration of basic Fibroblast Growth Factor (bFGF) ranging from 5 ng to 100 ng/ml. We found that bFGF effected both the morphology and mitotic potential of these cells. In addition, oligodendrocytes exposed to bFGF respond to 10% fetal bovine serum (FBS) by undergoing morphological changes that are quite different than naive oligodendrocytes exposed to 10% FBS, suggesting that bFGF causes some fundamental change in plasticity.
Hoffman
K L
KL
Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706, USA.
Duncan
I D
ID
D000818:Animals; D001345:Autoradiography; D002478:Cells, Cultured; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008938:Mitosis; D009836:Oligodendroglia / Q000166:cytology / Q000187:drug effects*; D013936:Thymidine / Q000494:pharmacology
D016222:Fibroblast Growth Factor 2; D013936:Thymidine