The mode of actions of glyceraldehyde-3-phosphate dehydrogenase identified as an immunoglobulin production stimulating factor.
Immunoglobulin production stimulating factor-II alpha, which enhances immunoglobulin production of human and mouse hybridomas was purified from cell lysate of human Burkitt's lymphoma, Namalwa cells, and identified as glyceraldehyde-3-phosphate dehydrogenase. The enhancement of immunoglobulin production with this enzyme was not linked with its enzymatic activity. The enzyme enhanced immunoglobulin productivity of transcription-suppressed hybridomas, but did not enhance that of translation-suppressed hybridomas. From these results, it is suggested that this enzyme takes part in the post-translational control or the enhancement of translation activity to stimulate immunoglobulin production of hybridomas.
Sugahara
T
T
Department of Biotechnology, College of Agriculture, Ehime University, Japan.
Shirahata
S
S
Sasaki
T
T
Murakami
H
H
D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D006801:Humans; D006825:Hybridomas; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000235:genetics; D007155:Immunologic Factors / Q000378:metabolism*; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
D007136:Immunoglobulins; D007155:Immunologic Factors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases