Evaluation of disk method for hippurate hydrolysis by Campylobacter species.
A disk method for hippurate hydrolysis was compared with the ninhydrin tube method by using 140 genetically confirmed Campylobacter strains. Results were similar for 129 (92%) strains when the inoculum size for the disk method was standardized. Six strains (4.2%) showed variable results by each method. Our results conflict with those obtained in studies by others, who found the two methods to be dissimilar.
Nicholson
M A
MA
Foodborne Diseases Laboratory Section, U.S. Department of Health and Human Services, Atlanta, Georgia 30333, USA.
Patton
C M
CM
D000818:Animals; D001431:Bacteriological Techniques / Q000592:standards; D002167:Campylobacter / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D006626:Hippurates / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D009555:Ninhydrin; D010641:Phenotype; D013045:Species Specificity
D006626:Hippurates; D009555:Ninhydrin; C030514:hippuric acid