M-cadherin localization in developing adult and regenerating mouse skeletal muscle: possible involvement in secondary myogenesis.
In this work, we investigated the distribution of the Ca(2+)-dependent cell adhesion molecule, M-cadherin, in mouse limb muscle during normal development and regeneration. Using two unrelated anti-M-cadherin peptide antibodies, we found scarce M-cadherin immunostaining during primary myogenesis (E12-E14) with no accumulation at areas of cell-cell contact. In contrast, the staining sharply increased in intensity at E16, remained high during secondary myogenesis (E16-P0) but disappeared soon after birth. During secondary myogenesis, M-cadherin was specifically accumulated at the characteristic sites of insertion of secondary myotubes in neighbouring primary myotubes. M-cadherin was also accumulated at the areas of contact between fusing secondary myoblasts and myotubes in vitro. In the adult normal and regenerating muscle, we did not detect M-cadherin accumulations at the surface of myofibres. All together, these observations suggest that M-cadherin is specifically involved in secondary myogenesis.
Cifuentes-Diaz
C
C
INSERM U. 153, CNRS ERS 64, Paris, France.
Nicolet
M
M
Alameddine
H
H
Goudou
D
D
Dehaupas
M
M
Rieger
F
F
Mège
R M
RM
D000595:Amino Acid Sequence; D000818:Animals; D015820:Cadherins / Q000032:analysis*; D002450:Cell Communication / Q000502:physiology; D002478:Cells, Cultured; D005314:Embryonic and Fetal Development / Q000502:physiology; D006614:Hindlimb / Q000196:embryology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D024510:Muscle Development; D009124:Muscle Proteins / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000254:growth & development / Q000502:physiology; D012038:Regeneration / Q000502:physiology*
D015820:Cadherins; D009124:Muscle Proteins; C070896:M-cadherin