title: Evaluation of a chemiluminescent molecular hybridization assay for the detection and quantitation of hepatitis B virus-DNA in serum.
abstract: The Digene Chemiluminescent Molecular Hybridization Assay for the detection and quantitation of hepatitis B virus DNA (HBV-DNA) in serum was tested by comparing it with the Abbott HBV-DNA assay, which uses a radiolabelled probe. For the values of clinical interest, a relationship was found between the results (in pg/ml) from the Digene (Y) and the Abbott (X) assays: Y = 7X + 5. The cut-off value to obtain positive results with a specificity of 100% (as determined by polymerase chain reaction), was found to be 15 pg/ml for the Digene test and 10 pg/ml for the Abbott test. It was concluded that the Digene Chemiluminescence Assay is a fast convenient test, which is suitable for routine use.
authors.affiliation: Laboratoire de Virologie, Hôpital de la Timone, Marseille, France.
authors.forename: X
authors.initials: X
authors.lastname: De Lamballerie
authors.forename: P
authors.initials: P
authors.lastname: Gallian
authors.forename: P H
authors.initials: PH
authors.lastname: De Micco
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral / Q000097:blood*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D008163:Luminescent Measurements; D015336:Molecular Probe Techniques; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral