title: The eco72IC gene specifies a trans-acting factor which influences expression of both DNA methyltransferase and endonuclease from the Eco72I restriction-modification system.
abstract: Eco72I from Escherichia coli RFL72 is a type-II restriction-modification (R-M) system recognizing and cleaving the sequence 5'-CAC decreases GTG-3'. The R-M genes are transcribed divergently and between the two genes is a small open reading frame codirectional to the R gene. This small ORF acts both to stimulate ENase expression and to depress DNA methyltransferase synthesis. The activity of beta Gal produced from the eco72IM::lacZ translational fusion increased tenfold, and eco72IR::lacZ translational fusion beta Gal activity decreased 130-fold when eco72IC was inactivated by a frameshift mutation. Analysis of nucleotide sequences of R-M systems, containing C genes, revealed a 5'-ACCTTATAGTC-3' consensus sequence upstream from the regulatory genes in all six analysed R-M systems. This sequence, named C-box, may play the role of an operator sequence.
authors.affiliation: Institute of Biotechnology FERMENTAS, Vilnius, Lithuania.
authors.forename: R
authors.initials: R
authors.lastname: Rimseliene
authors.forename: R
authors.initials: R
authors.lastname: Vaisvila
authors.forename: A
authors.initials: A
authors.lastname: Janulaitis
mesh_terms: D001483:Base Sequence; D016384:Consensus Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D016368:Frameshift Mutation; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D001616:beta-Galactosidase