title: Computer image sperm selection as a novel approach to subzonal insemination in the human.
abstract: Utilizing real-time computer image analysis, individual spermatozoa were selected using microaspiration. Selection criteria were based on potential hyperactivation motility characteristics; the amplitude of lateral head displacement > 7.5 microns, curvilinear velocity > 70 microns/s and linearity of < 30%. For this pilot study, 16 patients (eight in each group) were recruited. Using subzonal insemination (SUZI), up to five (mean = 4.4 +/- 0.3) spermatozoa selected using computer-image sperm selection (CISS) were micro-injected, or up to 15 (mean = 12.8 +/- 1.3 SD) unselected spermatozoa. In the group which utilized CISS, 28 out of 49 (57%) oocytes were fertilized compared with 13 out of 52 (25%) utilizing conventional SUZI (P < 0.04); polyspermy was 20% (n = 10) and 2% (n = 1) respectively. CISS with SUZI showed increased efficiency in achieving fertilization and is a novel approach to studying individual sperm function in a sperm egg bioassay where gamete ratios are close to unity.
authors.affiliation: Department of Obstetrics and Gynaecology, Queen's Medical Centre, Nottingham, UK.
authors.forename: S
authors.initials: S
authors.lastname: Green
authors.forename: S
authors.initials: S
authors.lastname: Fishel
authors.forename: J
authors.initials: J
authors.lastname: Hall
authors.forename: A
authors.initials: A
authors.lastname: Hunter
authors.forename: S
authors.initials: S
authors.lastname: Fleming
authors.forename: G
authors.initials: G
authors.lastname: Hobson
authors.forename: H
authors.initials: H
authors.lastname: Roe
authors.forename: K
authors.initials: K
authors.lastname: Dowell
authors.forename: S
authors.initials: S
authors.lastname: Thornton
authors.forename: L
authors.initials: L
authors.lastname: Klentzeris
mesh_terms: D000328:Adult; D003199:Computer Systems; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007315:Insemination, Artificial / Q000379:methods*; D008297:Male; D008845:Microinjections; D008875:Middle Aged; D010865:Pilot Projects; D013081:Sperm Motility; D013084:Sperm-Ovum Interactions; D013094:Spermatozoa; D013396:Suction; D015044:Zona Pellucida