title: Targeted disruption of mouse EGF receptor: effect of genetic background on mutant phenotype.
abstract: Gene targeting was used to create a null allele at the epidermal growth factor receptor locus (Egfr). The phenotype was dependent on genetic background. EGFR deficiency on a CF-1 background resulted in peri-implantation death due to degeneration of the inner cell mass. On a 129/Sv background, homozygous mutants died at mid-gestation due to placental defects; on a CD-1 background, the mutants lived for up to 3 weeks and showed abnormalities in skin, kidney, brain, liver, and gastrointestinal tract. The multiple abnormalities associated with EGFR deficiency indicate that the receptor is involved in a wide range of cellular activities.
authors.affiliation: Department of Genetics, Case Western Reserve University, Cleveland, OH 44106-4955, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: Threadgill
authors.forename: A A
authors.initials: AA
authors.lastname: Dlugosz
authors.forename: L A
authors.initials: LA
authors.lastname: Hansen
authors.forename: T
authors.initials: T
authors.lastname: Tennenbaum
authors.forename: U
authors.initials: U
authors.lastname: Lichti
authors.forename: D
authors.initials: D
authors.lastname: Yee
authors.forename: C
authors.initials: C
authors.lastname: LaMantia
authors.forename: T
authors.initials: T
authors.lastname: Mourton
authors.forename: K
authors.initials: K
authors.lastname: Herrup
authors.forename: R C
authors.initials: RC
authors.lastname: Harris
mesh_terms: D000015:Abnormalities, Multiple / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000002:abnormalities / Q000166:cytology; D002455:Cell Division; D004064:Digestive System / Q000166:cytology; D004065:Digestive System Abnormalities; D005314:Embryonic and Fetal Development; D066246:ErbB Receptors / Q000172:deficiency / Q000235:genetics* / Q000502:physiology*; D005260:Female; D018390:Gene Targeting; D006197:Hair / Q000002:abnormalities; D006720:Homozygote; D007668:Kidney / Q000166:cytology; D008168:Lung / Q000166:cytology; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010641:Phenotype; D012867:Skin / Q000166:cytology; D012868:Skin Abnormalities
chemical_list: D066246:ErbB Receptors