title: Modified amyloid beta protein ending at 42 or 40 with different solubility accumulates in the brain of Alzheimer's disease.
abstract: Serial extraction study of Alzheimer's disease (AD) and control brains revealed 4, 3.7 and 3 kD amyloid beta protein (A beta) species accumulated in AD brains. In the fractions extracted with TBS, 10% SDS and formic acid, considerable amounts of A beta species were recovered in SDS fractions besides TBS and formic acid fractions. Immunoblotting with several site-specific antibodies confirmed not only the presence of 4 kD A beta starting at the first amino acid of A beta but also 2 smaller A beta species with modification of their amino-termini in the highly resolutional Tris/Tricine gel system. A beta solubility using these solvents was associated with both modification of the amino-terminus and length of carboxyl-terminus of A beta. Especially, a large amount of modified A beta was found to be accumulated as forms with different solubility in AD brains.
authors.affiliation: Department of Neurology, Gunma University School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Harigaya
authors.forename: M
authors.initials: M
authors.lastname: Shoji
authors.forename: T
authors.initials: T
authors.lastname: Kawarabayashi
authors.forename: M
authors.initials: M
authors.lastname: Kanai
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Iizuka
authors.forename: Y
authors.initials: Y
authors.lastname: Igeta
authors.forename: T C
authors.initials: TC
authors.lastname: Saido
authors.forename: N
authors.initials: N
authors.lastname: Sahara
authors.forename: H
authors.initials: H
authors.lastname: Mori
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000276:immunology; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001923:Brain Chemistry; D015200:Fluoroimmunoassay; D006801:Humans; D015151:Immunoblotting
chemical_list: D016229:Amyloid beta-Peptides; D000911:Antibodies, Monoclonal