Enhancement of beta-galactosidase gene expression in rat pheochromocytoma cells by exposure to extremely low frequency magnetic fields.
Exposure of PC12-VG cells to an extremely low frequency magnetic field (ELFMF) enhanced the beta-galactosidase gene expression stimulated by treatment of the cells with forskolin. The enhancing effect of the ELFMF was inhibited by treatment of the cells with a specific inhibitor of PKC, calphostin C, as well as with the Ca2+ entry blockers nifedipin and dantrolen. Enhancement appeared within the first hour of a 4h forskolin treatment when the ELFMF was given at different times during culture. We speculate that exposure of PC12-VG cells to an ELFMF during the early response to forskolin treatment affects cell signal transduction, resulting in enhanced gene expression.
Ohtsu
S
S
Department of Radiation Genetics, Faculty of Medicine, Kyoto University, Japan.
Miyakoshi
J
J
Tsukada
T
T
Hiraoka
M
M
Abe
M
M
Takebe
H
H
D000310:Adrenal Gland Neoplasms / Q000201:enzymology; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D003620:Dantrolene / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000528:radiation effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000528:radiation effects*; D006801:Humans; D007700:Kinetics; D008280:Magnetics; D009281:Naphthalenes / Q000494:pharmacology; D009543:Nifedipine / Q000494:pharmacology; D016716:PC12 Cells; D010673:Pheochromocytoma / Q000201:enzymology; D011401:Promoter Regions, Genetic; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014162:Transfection; D014660:Vasoactive Intestinal Peptide / Q000235:genetics; D001616:beta-Galactosidase / Q000096:biosynthesis*
D009281:Naphthalenes; D011994:Recombinant Proteins; D005576:Colforsin; D014660:Vasoactive Intestinal Peptide; D011493:Protein Kinase C; D001616:beta-Galactosidase; D003620:Dantrolene; C058819:calphostin C; D009543:Nifedipine; D013755:Tetradecanoylphorbol Acetate