title: Tumor uptake of intravenously administered radiolabeled antibodies in ovarian carcinoma patients in relation to antigen expression and other tumor characteristics.
abstract: To study factors that possibly influence the heterogeneous tumor uptake of radiolabeled antibodies, tissues from 34 ovarian-carcinoma patients were obtained 2 to 8 days after i.v. injection with radiolabeled murine OV-TL3 or chimeric MOv18 (cMOv18). The tumor uptake and the ratio of tumor to normal tissue (T/NT) were studied in relation to the histopathological classification, prior treatment, site of tumor, time interval, antigen expression, volume percentage of (malignant) epithelium in the tumor tissue, and the size of the tumor. The results of immunoscintigraphy were also included. In addition, autoradiography using storage phosphor technology was performed on tissue sections from patients injected with iodine-labeled cMOv18. Tumor uptake varied largely, not only between patients, but also between tumor deposits within the same patient. Uptake of OV-TL3 F(ab')2 was higher than of cMOv18 F(ab')2, but the T/NT ratios were similar. The antibody uptake was positively correlated with the pattern of antigen expression and inversely correlated with the time interval between injection and surgery. No correlation was observed with any of the other factors studied. The visibility with immunoscintigraphy was related to the size of the detected lesion, but not to the other factors studied. Autoradiography showed that antibodies preferentially localized in areas with cancer cells, which were immunohistochemically positive for MOv18. In areas with weak antigen expression, autoradiography revealed less activity. The antigen expression by the tumor is an important factor for estimation of the tumor uptake of radiolabeled antibodies.
authors.affiliation: Department of Obstetrics and Gynaecology, Free University Hospital, Amsterdam, The Netherlands.
authors.forename: M R
authors.initials: MR
authors.lastname: Buist
authors.forename: P
authors.initials: P
authors.lastname: Kenemans
authors.forename: C F
authors.initials: CF
authors.lastname: Molthoff
authors.forename: J C
authors.initials: JC
authors.lastname: Roos
authors.forename: W
authors.initials: W
authors.lastname: Den Hollander
authors.forename: M
authors.initials: M
authors.lastname: Brinkhuis
authors.forename: J P
authors.initials: JP
authors.lastname: Baak
mesh_terms: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000378:metabolism*; D001345:Autoradiography; D002277:Carcinoma / Q000000981:diagnostic imaging* / Q000276:immunology / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007275:Injections, Intravenous; D016014:Linear Models; D010051:Ovarian Neoplasms / Q000000981:diagnostic imaging* / Q000276:immunology / Q000473:pathology; D010053:Ovary / Q000000981:diagnostic imaging* / Q000276:immunology; D011877:Radionuclide Imaging
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm