Alternative RNA of epsilon transcripts produces mRNAs encoding two membrane and four secreted IgE isoforms.
We elucidated a series of alternatively spliced human epsilon mRNAs by employing a human epsilon-specific RT-PCR strategy. Two mRNA isoforms provide for the expression of distinct membrane IgE proteins, the common form of which contains a novel 56-amino-acid sequence after the last epsilon constant-region domain. Other mRNAs encode, in addition to the classic secreted epsilon chain, at least three novel epsilon secreted proteins that lack a transmembrane domain and possess distinctive C-terminal features. Furthermore, the expression of these epsilon mRNA isoforms is differentially regulated by stimuli such as interleukin-10, Fc epsilon RII cross-linking and aromatic hydrocarbons found in diesel exhaust.
Saxon
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Hart and Louise Lyon Laboratory, Department of Medicine, UCLA School of Medicine 90024-1680, USA.
Max
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Diaz-Sanchez
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Zhang
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D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D006801:Humans; D007073:Immunoglobulin E / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*
D012333:RNA, Messenger; D007073:Immunoglobulin E