A role for platelet release of serotonin in the initiation of contact sensitivity.
Finding contact sensitivity (CS) responses that were fairly normal in ear swelling, and in serotonin (5-HT) dependence in mast-cell-deficient mice, led to experiments to determine whether platelets supplemented mast cells as a source of 5-HT in CS. Severe depletion of platelets, and consequently blood 5-HT, with antiplatelet antibody, strongly inhibited CS, especially in mast-cell-deficient mice, suggesting that platelets supplemented mast cells. Furthermore, human platelets sensitized in vitro with anti-(tri-nitro-phenyl) IgE, and transferred intravenously together with isolated late-acting effector T cells, provided CS initiation due to local 5-HT release. Similar, IgE-dependent in vitro release of 5-HT was C dependent. These findings establish the importance of antigen-specific platelet release of 5-HT in CS initiation.
Askenase
P W
PW
Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520-8013, USA.
Geba
G P
GP
Levin
J
J
Ratzlaff
R E
RE
Anderson
G M
GM
Ushio
H
H
Ptak
W
W
Matsuda
H
H
D000661:Amphetamine / Q000494:pharmacology; D000818:Animals; D001792:Blood Platelets / Q000187:drug effects / Q000378:metabolism*; D017449:Dermatitis, Allergic Contact / Q000503:physiopathology*; D007073:Immunoglobulin E / Q000276:immunology; D008407:Mast Cells / Q000473:pathology; D051379:Mice; D008817:Mice, Mutant Strains; D011817:Rabbits; D017455:Receptors, IgE / Q000276:immunology; D012701:Serotonin / Q000097:blood / Q000378:metabolism*; D012867:Skin / Q000473:pathology; D014301:Trinitrobenzenes / Q000276:immunology
D017455:Receptors, IgE; D014301:Trinitrobenzenes; D012701:Serotonin; D007073:Immunoglobulin E; D000661:Amphetamine