On-line monitoring of glucose, glutamate and glutamine during mammalian cell cultivations.
Amperometric biosensors (based on rhodinised carbon electrodes) for glucose, glutamine and glutamate were constructed. The sensors were incorporated into a three cell parallel FIA system and used to monitor the three analytes on-line during two mammalian cell perfusion cultures. All measurements were made simultaneously from undiluted media sample. Use of the FIA system enabled easy and rapid exchange of the sensors, during cultivation. The inclusion of a calibration step, regularly for all sensors, helped to maintain the accuracy of all measurements. Comparison with off-line measurements indicated that all three biosensors operated successfully, providing accurate information.
White
S F
SF
Cranfield Biotechnology Centre, Cranfield University, Bedfordshire, UK.
Turner
A P
AP
Biltewski
U
U
Bradley
J
J
Schmid
R D
RD
D015374:Biosensing Techniques; D002478:Cells, Cultured; D005947:Glucose / Q000032:analysis*; D018698:Glutamic Acid / Q000032:analysis*; D005973:Glutamine / Q000032:analysis*; D006801:Humans
D005973:Glutamine; D018698:Glutamic Acid; D005947:Glucose