Purification and characterization of the restriction endonuclease AvcI from Actinomyces cristalomycini.
The restriction endonuclease AvcI, an isoschizomer of Sau96I [Sussenbach et al., Nucleic Acids Res. 5 (1978) 1153-1163], was purified from Actinomyces cristalomycini. AvcI recognizes a 5-bp palindromic sequence, 5'-G decreases GNCC and cleaves it after the first G residue producing a 3-nucleotide 5'-overhang.
Rutkowska
S M
SM
Department of Microbiology, University of Gdańsk, Poland.
Skowron
P M
PM
Podhajska
A J
AJ
D000190:Actinomyces / Q000201:enzymology*; D001483:Base Sequence; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
D009838:Oligodeoxyribonucleotides; D004247:DNA; D004262:DNA Restriction Enzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C032262:GGNCC-specific type II deoxyribonucleases