[Effect of low dose rhTNF-alpha on immune function of lymphocytes in burned rats].
SD rats (200-230 g body weight) were randomly divided into three groups: (1) sham burn; (2) burn; (3) burn plus rhTNF-alpha. Third degree burn of 30% TBSA on the trunk was produced in groups 2 and 3. Intravenous rhTNF-alpha(10 micrograms/kg) was administered 6 hours postburn and once daily thereafter for 5 days in group 3. At 5 days postburn, splenic lymphocytes obtained from all the animals were used for the determination of distribution of T lymphocyte subsets with flowcytic analysis and activity of IL-2(Gills method). The results indicated that low dose rhTNF-alpha in vivo could improve distribution of splenic T lymphocyte subsets in burned rats by increasing the numbers of W3/25(Th) and decreasing the number of OX-8(Ts), and the ratio of W3/25/OX-8 was raised. In vitro the proliferation response of T lymphocytes to ConA was enhanced and the activity of IL-2 was increased by 108%, 149% and 168%, respectively, in group 3 compared with that in group 2. However, a dose-dependent effect, in that a small dose (< or = 10 ng/2.5 x 10(6) cells) of rhTNF-alpha improved the immune function of T lymphocytes, while a higher dose inhibited T lymphocyte immune function. These findings showed that rhTNF-alpha improved not only the distribution of T lymphocyte subsets but also their immune activity.
Su
B
B
Burn Institute, Changhai Hospital, Second Military Medical University, Shanghai.
Xue
J
J
Chen
Y
Y
D000818:Animals; D002056:Burns / Q000276:immunology*; D016516:CD4-CD8 Ratio; D007275:Injections, Intravenous; D007376:Interleukin-2 / Q000096:biosynthesis; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage / Q000494:pharmacology*
D007376:Interleukin-2; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha