Quantitation of the protein content of diphtheria and tetanus toxoids by the Biuret method during production of combined vaccines.
The protein content of 15 batches of diphtheria toxoid and 12 batches of tetanus toxoid was measured by Biuret method and was compared to the values obtained by the Kjeldahl method. Statistical analysis did not show any difference between the two methods. The Biuret method, is specific, easy to carry out and takes less time for protein estimation of purified toxoids during production of combined vaccines.
Shrivastaw
K P
KP
Biochemistry Laboratory, Central Research Institute, Kasauli, India.
Jhamb
S S
SS
Kumar
A
A
D002623:Chemistry Techniques, Analytical / Q000379:methods; D004168:Diphtheria Toxoid / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D013745:Tetanus Toxoid / Q000737:chemistry*; D017778:Vaccines, Combined / Q000737:chemistry
D004168:Diphtheria Toxoid; D011506:Proteins; D013745:Tetanus Toxoid; D017778:Vaccines, Combined