title: Molecular cloning of human liver sulfite oxidase.
abstract: A 2.4 kilobase cDNA clone of human sulfite oxidase was isolated from a human liver cDNA library in lambda gt10. Comparison of three sulfite oxidase sequences to several plant and fungal nitrate reductase sequences reveals a single conserved cysteine with highly conserved flanking sequences. The conserved cysteine is postulated to be a ligand of molybdenum in sulfite oxidase and nitrate reductase.
authors.affiliation: Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Garrett
authors.forename: D B
authors.initials: DB
authors.lastname: Bellissimo
authors.forename: K V
authors.initials: KV
authors.lastname: Rajagopalan
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000302:isolation & purification*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D050862:Oxidoreductases Acting on Sulfur Group Donors