Cholinergic modulation of immunoglobulin secretion from avian plasma cells: the role of cyclic mononucleotides.
The chicken Harderian (lacrimal) gland contains an abundance of plasma cells in the interstitium of the gland that secrete IgG, IgM, and IgA. In in vitro preparations of this gland, the cholinergic agonist carbachol causes a transient increase in the secretion rate of IgG above a basal level of secretion. We have investigated the effects of the cyclic mononucleotides cAMP and cGMP on this secretagogue response. Pretreatment with 20 microM forskolin or 1 mM dibutyryl cAMP abolished the carbachol-induced secretory response. When the gland was isolated in normal media and then treated with either forskolin or dibutyryl cAMP, there was no change in the baseline secretion rate. cGMP at either 10 microM or 1.0 mM did not affect the baseline secretion rate, nor did it have an effect on the carbachol response. We postulate that muscarinic receptor activation leads to a calcium influx that in turn leads to an increased secretion rate of IgG. The opposing effects of elevating cAMP and cGMP are discussed in the context of this model of cholinergic activation of avian plasma cells.
Cameron
R H
RH
Department of Physiology and Biophysics, SUNY Stony Brook, NY 11794-8661, USA.
Walcott
B
B
Claros
N
N
Mendel
K
K
Brink
P R
PR
D000818:Animals; D002217:Carbachol / Q000494:pharmacology*; D002645:Chickens; D046508:Culture Techniques; D000242:Cyclic AMP / Q000502:physiology*; D006152:Cyclic GMP / Q000502:physiology*; D006243:Harderian Gland / Q000166:cytology; D007074:Immunoglobulin G / Q000378:metabolism*; D010277:Parasympathomimetics / Q000494:pharmacology*; D010950:Plasma Cells / Q000378:metabolism*; D012636:Secretory Rate / Q000187:drug effects
D007074:Immunoglobulin G; D010277:Parasympathomimetics; D002217:Carbachol; D000242:Cyclic AMP; D006152:Cyclic GMP