Cloning of the rat angiotensin II type 2 receptor gene and identification of its functional promoter region.
We have cloned the rat angiotensin II receptor type 2 (AT2) gene, whose physiological function remains unclear. Sequence analysis indicated that exons 1 and 2 exist in the 5'-untranslated region and the initiation codon ATG is located in exon 3. The 1.6-kb genomic fragment at positions -1567 to +26 relative to the putative transcription start site was found to contain a functional promoter region using transient chloramphenicol acetyltransferase assay. This is the first report demonstrating the nucleotide sequence of the promoter region of this gene.
Kobayashi
S
S
Second Department of Internal Medicine, Yokohama City University, School of Medicine, Japan.
Ohnishi
J
J
Nibu
Y
Y
Nishimatsu
S
S
Umemura
S
S
Ishii
M
M
Murakami
K
K
Miyazaki
H
H
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005091:Exons; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D017207:Rats, Sprague-Dawley; D011945:Receptors, Angiotensin / Q000737:chemistry / Q000235:genetics*
D011945:Receptors, Angiotensin