Cloning and characterization of the 5'-flanking region of metallothionein-I gene from Chinese hamster ovary cells.
The 5'-flanking region of the Chinese hamster ovary metallothionein-I gene (MT-I) was isolated and sequenced. This region contains several cis-regulatory sequences, including metal-responsive element (MRE), AP1-binding site, Sp1-binding site and glucocorticoid-responsive element (GRE). These elements are located within 250 bases upstream of the transcription initiation site, as determined by primer extension. This region was fused with a reporter gene that could be activated by the challenge of cadmium only when the MREs were present. Thus, the isolated DNA is the promoter region of the CHO MT-I gene.
Yu
C W
CW
Lin
L Y
LY
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D004247:DNA / Q000302:isolation & purification; D004268:DNA-Binding Proteins; D008668:Metallothionein / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics; D000097809:Transcription Factor MTF-1
D004247:DNA; D004268:DNA-Binding Proteins; D008668:Metallothionein; D014157:Transcription Factors; D000097809:Transcription Factor MTF-1