title: Determination of the anticancer drug metabolite WR1065 using pre-column derivatization and diode laser induced fluorescence detection.
abstract: A liquid chromatographic (LC) procedure using alumina as stationary phase in both the pre- and the analytical column, is reported for the determination of WR1065, the active metabolite of the amino- and thiol-containing anticancer drug WR2721. After pre-column derivatization of the thiol group, the analyte is determined by LC with diode laser induced fluorescence detection in the near-infrared. Selective removal of excess label is achieved by means of column switching; it allows the detection of 5 x 10(-9) M WR1065 in water and 10-fold diluted, deproteinated plasma samples. The detection limit is determined by the derivatization reaction and not by the fluorescence detection of the labelled analyte. Endogeneous thiols do not interfere.
authors.affiliation: Department of General and Analytical Chemistry, Free University, Amsterdam, The Netherlands.
authors.forename: A J
authors.initials: AJ
authors.lastname: Mank
authors.forename: E J
authors.initials: EJ
authors.lastname: Molenaar
authors.forename: C
authors.initials: C
authors.lastname: Gooijer
authors.forename: H
authors.initials: H
authors.lastname: Lingeman
authors.forename: N H
authors.initials: NH
authors.lastname: Velthorst
authors.forename: U A
authors.initials: UA
authors.lastname: Brinkman
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002855:Chromatography, Thin Layer; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007834:Lasers; D008624:Mercaptoethylamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D011837:Radiation-Protective Agents / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D000970:Antineoplastic Agents; D007202:Indicators and Reagents; D008624:Mercaptoethylamines; D011837:Radiation-Protective Agents; C020174:N-(2-mercaptoethyl)-1,3-diaminopropane