Neutralization of feline immunodeficiency virus by polyclonal feline antibody: simultaneous involvement of hypervariable regions 4 and 5 of the surface glycoprotein.
Sites involved in antibody-mediated neutralization of feline immunodeficiency virus were mapped by reciprocal exchange of envelope fragments or amino acids between molecular clones of feline immunodeficiency virus with different susceptibilities to neutralization by a polyclonal cat serum. Combinations of mutations within HV-4 or within HV-4 and HV-5 changed the susceptibility of the viruses to neutralizing antibody.
Siebelink
K H
KH
Institute of Virology, Erasmus University Rotterdam, The Netherlands.
Huisman
W
W
Karlas
J A
JA
Rimmelzwaan
G F
GF
Bosch
M L
ML
Osterhaus
A D
AD
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002460:Cell Line; D003001:Cloning, Molecular; D004285:Dogs; D007106:Immune Sera; D016089:Immunodeficiency Virus, Feline / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000276:immunology*; D009500:Neutralization Tests; D017354:Point Mutation; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D007106:Immune Sera; D008562:Membrane Glycoproteins; D014764:Viral Proteins