title: Comparison of tribromoethanol, ketamine/acetylpromazine, Telazol/xylazine, pentobarbital, and methoxyflurane anesthesia in HSD:ICR mice.
abstract: Variation in the duration of surgical anesthesia in mice prompted an evaluation of various commonly used anesthetics. Using biotelemetric technology, we evaluated the effects of six anesthetic regimens (tribromoethanol, ketamine and acetylpromazine in combination, Telazol and xylazine in two combinations, pentobarbital, and methoxyflurane) on temperature and activity. Six groups of four male HSD:ICR mice received one of the anesthetic regimens or an equivalent volume of saline. Induction time (time from anesthetic administration until righting reflex loss) and duration of anesthesia (loss of response to interdigital toe pinch) were evaluated. Methoxyflurane and both doses of Telazol combinations resulted in the shortest and most repeatable induction times. None of the mice in the ketamine/acetylpromazine- and pentobarbital-treated groups lost the interdigital toe pinch reflex. Duration of anesthesia was superior in the two Telazol/xylazine-treated groups. A direct correlation existed between duration of anesthesia and magnitude and duration of temperature reduction. Duration of anesthesia can be used to predict extent of hypothermia.
authors.affiliation: Division of Veterinary Services, Food and Drug Administration, Bethesda, MD 20892, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Gardner
authors.forename: J A
authors.initials: JA
authors.lastname: Davis
authors.forename: P J
authors.initials: PJ
authors.lastname: Weina
authors.forename: B
authors.initials: B
authors.lastname: Theune
mesh_terms: D000075:Acepromazine; D000768:Anesthesia, General / Q000662:veterinary*; D018681:Anesthetics, General; D000818:Animals; D001831:Body Temperature; D004338:Drug Combinations; D000431:Ethanol / Q000031:analogs & derivatives; D007649:Ketamine; D008297:Male; D008733:Methoxyflurane; D051379:Mice; D008813:Mice, Inbred ICR; D010424:Pentobarbital; D013686:Telemetry; D014991:Xylazine
chemical_list: D018681:Anesthetics, General; D004338:Drug Combinations; C062527:tribromoethanol; D014991:Xylazine; D008733:Methoxyflurane; D000431:Ethanol; D000075:Acepromazine; D007649:Ketamine; D010424:Pentobarbital