title: Unbudded G2 as well as G1 arrest in the stationary phase of the basidiomycetous yeast Cryptococcus neoformans.
abstract: Stationary-phase cells of Cryptococcus neoformans displayed two morphological characteristics: virtually all the cells were unbudded even in the early stationary phase and even when grown in rich media, and average cell size increased from that of exponential-phase cells. DNA contents for small and large stationary-phase cells were determined by quantitative fluorescence microscopy after DNA staining with propidium iodide or DAPI. Small cells contained G1 DNA, whereas large unbudded cells had either a G2 or G1 DNA content, indicating that Cr. neoformans can enter into the stationary phase from either the G1 or G2 period.
authors.affiliation: Department of Fungal Infections, Chiba University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Takeo
authors.forename: R
authors.initials: R
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Miyaji
authors.forename: K
authors.initials: K
authors.lastname: Nishimura
mesh_terms: D003455:Cryptococcus neoformans / Q000254:growth & development* / Q000378:metabolism; D004271:DNA, Fungal / Q000032:analysis; D016193:G1 Phase; D016195:G2 Phase
chemical_list: D004271:DNA, Fungal