Diisopropyl phosphorofluoridate (DFP) treatment alters calcium-activated proteinase activity and cytoskeletal proteins of the hen sciatic nerve.
Diisopropyl phosphorofluoridate (DFP) produces delayed neurotoxicity (OPIDN) in hens that is characterized by peripheral and central axonal degeneration. DFP administration resulted in mCANP activity inhibition in sciatic nerve and significant decrease in total NF-H, phosphorylated NF-H, vimentin, GFAP, tubulin, and tau. The degradation of cytoskeletal proteins even in the presence of decreased CANP activity may be ascribed to the release of intracellular Ca2+, elevation of other proteinase activity, or modification of cytoskeletal proteins resulting in their increased susceptibility in OPIDN.
Gupta
R P
RP
Duke University Medical Center, Department of Pharmacology, Durham, NC, USA.
Abou-Donia
M B
MB
D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D001923:Brain Chemistry / Q000187:drug effects; D002154:Calpain / Q000037:antagonists & inhibitors*; D002645:Chickens; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D005260:Female; D007531:Isoflurophate / Q000633:toxicity*; D009422:Nervous System Diseases / Q000139:chemically induced / Q000378:metabolism; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism*; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism
D000911:Antibodies, Monoclonal; D003598:Cytoskeletal Proteins; D007531:Isoflurophate; D002154:Calpain