Characterization of cDNAs coding for glucose phosphate isomerase and phosphoglycerate kinase in Chinese hamster ovary cell line CHO-K1 and identification of defects in R1.1.7, a glycolysis-deficient variant of CHO-K1.
Full-length cDNAs for glucose phosphate isomerase (GPI) and phosphoglycerate kinase (PGK) of the Chinese hamster ovary cell line CHO-K1 have been characterized using RT-PCR and cycle sequencing of the PCR-amplified templates. Mutations in both genes have been identified in a glycolysis-deficient Chinese hamster ovary cell line, R1.1.7, derived from CHO-K1 cells.
Hassan-Walker
A F
AF
Wellcome Laboratory For Molecular Genetics, Division of Biochemistry and Molecular Biology, UMDS, London, UK.
Morgan
M J
MJ
Faik
P
P
D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D006224:Cricetinae; D018076:DNA, Complementary / Q000302:isolation & purification; D005260:Female; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D016133:Polymerase Chain Reaction
D018076:DNA, Complementary; D010735:Phosphoglycerate Kinase; D005956:Glucose-6-Phosphate Isomerase