A novel method employing polymerase chain reaction to disrupt genes lacking convenient restriction enzyme sites in yeast.
A novel method employing polymerase chain reaction was developed for the disruption of yeast genes lacking convenient restriction enzyme sites. The method was found to be easy and effective. Using this method, a yeast YKE2 gene (a yeast homolog of murine k-region expressed genes) were successfully disrupted by replacement of HIS3 marker gene.
Wu
M
M
Institute of Molecular and Cell Biology, National University of Singapore.
Shang
H S
HS
Yeong
C Y
CY
Tan
P H
PH
Lu
Z X
ZX
Wong
S M
SM
D003001:Cloning, Molecular; D005800:Genes, Fungal / Q000235:genetics*; D005819:Genetic Markers; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping; D015003:Yeasts / Q000235:genetics*
D005819:Genetic Markers