Primary cytotoxic response of bovine peripheral blood leukocytes to parainfluenza type 3 virus infection.
Peripheral blood mononuclear cells were isolated from calves following Parainfluenza Type-3 (PI-3) virus infection and incubated with autologous and non-autologous PI-3 virus-infected muscle cells in a 4 h chromium release assay. Peaks of cytotoxicity, which ranged from 12% to 53% were observed between Days 6 and 9 post infection, with an effector to target cell ratio of 100:1. Killing of PI-3 virus-infected or uninfected mismatched muscle cells was never more than 5%.
Bamford
A I
AI
Veterinary Sciences Division, Stormont, Belfast, UK.
Adair
B M
BM
Foster
J C
JC
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic / Q000276:immunology*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000821:virology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006386:Hemagglutination Tests / Q000662:veterinary; D015395:Histocompatibility Antigens Class I / Q000032:analysis / Q000096:biosynthesis; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism / Q000821:virology; D010224:Parainfluenza Virus 3, Human / Q000254:growth & development / Q000276:immunology*; D018184:Paramyxoviridae Infections / Q000276:immunology / Q000662:veterinary*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000914:Antibodies, Viral; D015395:Histocompatibility Antigens Class I