Prolonged exposure of hamsters to cold changes the levels of G proteins in brown adipose tissue plasma membranes.
The levels of G proteins in plasma membranes prepared from brown adipose tissue of control and cold-exposed hamsters were determined by quantitative immunoblotting and competitive ELISA. Prolonged (four weeks) exposure of hamsters to cold decreased significantly the total content of the alpha subunits of the stimulatory (Gs alpha) as well as inhibitory (Gi alpha (1,2)) G proteins. Interestingly, the reduction in the Gs alpha content was solely due to a large reduction in the content of the short (45 kDa) isoform of Gs alpha, while the level of the long (52 kDa) isoform of Gs alpha remained unchanged. The level of the beta subunit of G protein was decreased comparably to the reduction in the total content of the alpha subunits. Cold-induced alterations in the G protein network associated with plasma membranes of brown adipose tissue were accompanied by changed characteristics of AlF(4-)-sensitive adenylyl cyclase activity.
Kvapil
P
P
Wallenberg Laboratory for Cardiovascular Research, Gothenburg University, Sweden.
Novotny
J
J
Ransnäs
L A
LA
D000262:Adenylyl Cyclases / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000737:chemistry*; D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D003080:Cold Temperature; D006224:Cricetinae; D019204:GTP-Binding Proteins / Q000032:analysis*; D008647:Mesocricetus; D011817:Rabbits
D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases