title: Immune response in the hamster: definition of a novel IgG not expressed in all hamster strains.
abstract: A new IgG isotype is described in serum from Syrian hamsters. This 7S-IgG is called IgG3 and was isolated from IgG1 and IgG2 because of its great affinity for protein A. The unique antigenic determinants of IgG3 were identified with a specific rabbit antisera. IgG3 is the least expressed IgG subclass in Syrian hamsters, but serum levels increase more than 10-fold after immunization or infection. Although found in all tested outbred strains, IgG3 is expressed in only some of the commercially available inbred strains of Syrian hamsters. Five inbred hamster strains were examined, and in three strains (CB, LHC and MHA) IgG3 was not detected in normal serum or in immune serum, indicating serum levels at least 100-fold less than other normal inbred/outbred hamsters. The results of breeding experiments suggests a single gene defect is responsible for this non-expression of IgG3. Immunodeficiency was not associated with this IgG3 deficiency. Selective deficiencies of immunoglobulin classes/subclasses in experimental animals are rare. The evolution of a similar IgG3 deficiency in these three hamster strains during inbreeding suggests a novel and efficient mechanism for regulation of IgG3 synthesis in the Syrian hamster.
authors.affiliation: Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Coe
authors.forename: R F
authors.initials: RF
authors.lastname: Schell
authors.forename: M J
authors.initials: MJ
authors.lastname: Ross
mesh_terms: D000818:Animals; D001947:Breeding; D006224:Cricetinae; D003433:Crosses, Genetic; D000939:Epitopes / Q000276:immunology; D005260:Female; D017099:IgG Deficiency / Q000235:genetics; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000097:blood* / Q000235:genetics / Q000276:immunology; D007518:Isoantibodies / Q000097:blood* / Q000235:genetics / Q000276:immunology; D008297:Male; D008647:Mesocricetus / Q000235:genetics / Q000276:immunology*; D010641:Phenotype; D013045:Species Specificity; D014210:Treponema pallidum / Q000276:immunology
chemical_list: D000939:Epitopes; D007074:Immunoglobulin G; D007518:Isoantibodies