7-Deaza purine bases offer a higher ion stability in the analysis of DNA by matrix-assisted laser desorption/ionization mass spectrometry.
Oligodeoxynucleotides which contain 7-deaza analogues of the normal purine nucleotides have been synthesized both enzymatically and chemically. When subjected to matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) analysis, the modified samples offer both higher stability and increased sensitivity compared to otherwise identical unmodified oligodeoxynucleotides. In view of these observations, models for the fragmentation of oligodeoxynucleotides in MALDI-MS with positive ion detection mode are presented. Additionally, the potential use of 7-deaza purine nucleotides in the MALDI-MS analysis of DNA sequencing reactions is discussed.
Kirpekar
F
F
Department of Molecular Biology, University of Odense, Denmark.
Nordhoff
E
E
Kristiansen
K
K
Roepstorff
P
P
Hahner
S
S
Hillenkamp
F
F
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D007834:Lasers; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis; D011687:Purines / Q000737:chemistry*
D009841:Oligonucleotides; D011687:Purines; D004247:DNA