Early replication block of human immunodeficiency virus type 1 in monkey cells.
The genetic and functional basis of the replication-defective nature of human immunodeficiency virus type 1 (HIV-1) in monkey cells was studied. By the generation and characterization of chimeras between HIV-1 and simian immunodeficiency virus, the sequence encompassing the 3' half of the long terminal repeat, gag and pol genes of HIV-1 was found to be responsible for the growth restriction. Early and late phases of HIV-1 replication in monkey cells were analysed in detail using several assay systems: transfection/coculture, transcomplementation between various proviral clones carrying the CAT gene and effector clones and evaluation of transcription and reverse transcription. All the data were consistent with the notion that HIV-1 replication is blocked at a very early stage(s) such as uncoating and/or reverse transcription in monkey cells.
Shibata
R
R
Department of Viral Oncology, Kyoto University, Japan.
Sakai
H
H
Kawamura
M
M
Tokunaga
K
K
Adachi
A
A
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D017931:DNA Primers; D004279:DNA, Viral / Q000096:biosynthesis*; D003673:Defective Viruses / Q000235:genetics / Q000502:physiology; D015750:Genes, gag; D015751:Genes, pol; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D000882:Haplorhini; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000821:virology; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D015302:Simian Immunodeficiency Virus / Q000235:genetics; D014158:Transcription, Genetic; D014779:Virus Replication
D017931:DNA Primers; D004279:DNA, Viral