title: Biochemical characterization of VspI methyltransferase.
abstract: The gene (vspIM) encoding VspI methyltransferase (MTase) has previously been cloned and sequenced, and shown to belong to the gamma class of m6-adenine MTases [Degtyarev et al., Nucleic Acids Res. 21 (1993) 2015]. Here it is shown that the MTase modifies the third adenine within the recognition sequence 5'-ATTAAT-3'.
authors.affiliation: SibEnzyme, Novosibirsk, Russia.
authors.forename: S Kh
authors.initials: SKh
authors.lastname: Degtyarev
authors.forename: E A
authors.initials: EA
authors.lastname: Prikhod'ko
authors.forename: N I
authors.initials: NI
authors.lastname: Rechkunova
authors.forename: G G
authors.initials: GG
authors.lastname: Prikhod'ko
authors.forename: V N
authors.initials: VN
authors.lastname: Krasnykh
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D005798:Genes, Bacterial; D007700:Kinetics; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific) / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D011994:Recombinant Proteins; C081230:VspI methylase; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific)