title: Monitoring human risk and exposure to trinitrotoluene (TNT) using haemoglobin adducts as biomarkers.
abstract: Two studies were carried out in a trinitrotoluene (TNT) plant using TNT haemoglobin (TNT-Hb) adduct as a biomarker to study dose-adduct and adduct-response relationships. In the first study, TNT-Hb adduct levels were determined in 117 TNT-exposed workers in different working sites with different exposure conditions. External exposure was calculated from the inhaled air concentration plus skin contamination. TNT-Hb adduct levels in blood were significantly correlated with their external exposure to TNT. Two methods, HPLC-UV and CI-ELISA, were developed for measuring TNT-Hb adduct: good correlations (r = 0.77 and 0.86) were found between these 2 methods. In the second study, TNT cataract was used as an indicator of health effects. The prevalence of cataract and the degree of lenticular damage increased with the increase of blood TNT-Hb level.
authors.affiliation: Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Liu
authors.forename: M
authors.initials: M
authors.lastname: Yao
authors.forename: J L
authors.initials: JL
authors.lastname: Fang
authors.forename: Y W
authors.initials: YW
authors.lastname: Wang
mesh_terms: D002386:Cataract / Q000209:etiology; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D016273:Occupational Exposure / Q000009:adverse effects; D011485:Protein Binding; D018570:Risk Assessment; D014303:Trinitrotoluene / Q000097:blood* / Q000378:metabolism*
chemical_list: D006454:Hemoglobins; D014303:Trinitrotoluene