Biologic characteristics of T1 papillary bladder cancer. Flow cytometric study of paraffin-embedded material.
DNA ploidy, proliferative activity (evaluated in terms of the proliferative index [PI]) and epidermal growth factor receptors (EGF-R) expression in primary bladder cancer in 52 patients were studied by means of flow cytometry and immunohistochemistry. Forty of the 52 tumors yielded evaluable DNA histograms: 22 were diploid (55%) and 18 aneuploid (45%) (median DNA index = 1.5). Ninety-five percent of the tumors were positive for EGF-R expression. The median PI value of the entire case series, estimated with a mathematical model, was 5.4%, or 6.7% in diploid tumors and 12.1% in aneuploid ones. EGF-R expression was higher in aneuploid than diploid tumors. Most of the well-differentiated tumors were diploid, while aneuploid populations and positivity for EGF-R expression were more frequent in poorly and moderately differentiated tumors. No differences in proliferative activity were observed in relation to grade. Overexpression of EGF-R in aneuploid tumors and a relationship between it, proliferative activity and grading were observed. The disease-free survival rates were 72% and 91% for patients with aneuploid and diploid tumors, respectively.
Bucci
B
B
Laboratory of Experimental Chemotherapy, Regina Elena Cancer Institute, Rome, Italy.
Pansadoro
V
V
De Paula
F
F
Florio
A
A
Carico
E
E
Zupi
G
G
Vecchione
A
A
D000782:Aneuploidy; D002291:Carcinoma, Papillary / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics; D002455:Cell Division; D004247:DNA / Q000032:analysis; D018572:Disease-Free Survival; D066246:ErbB Receptors / Q000032:analysis; D005434:Flow Cytometry; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D016612:Paraffin Embedding; D012189:Retrospective Studies; D001749:Urinary Bladder Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics
D004247:DNA; D066246:ErbB Receptors