title: Ultrastructure of the cell wall of Schizosaccharomyces pombe following treatment with various glucanases.
abstract: The ultrastructure of isolated cell walls of Schizosaccharomyces pombe was studied by electron microscopy after treatment with the following purified enzymes: endo-beta-(1-->3)-glucanase, endo-beta-(-->6)-glucanase, and endo-alpha-(1-->3)-glucanase produced by Bacillus circulans; exo-beta-(1-->3)-glucanase and endo-beta-(1-->3)-glucanase produced by Schizosaccharomyces japonicus var. versatilis. The exo-beta-(1-->3)-glucanase had no detectable effect on the walls, but amorphous wall material was removed by action of the endo-beta-(1-->3)- and endo-beta-(1-->6)-glucanases of B. circulans to reveal a wall component consisting of densely interwoven microfibrils. The fibrils were hydrolyzed by treatment with the Schiz. japonicus endo-beta-(1-->3)-glucanase followed by B. circulans endo-alpha-(1-->3)-glucanase--suggesting that they were composed of -beta-(1-->3)-linked glucan and alpha-(1-->3)-linked glucan. The presence of a fibrillar component in untreated walls was evident after negative staining.
authors.affiliation: Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Kopecká
authors.forename: G H
authors.initials: GH
authors.lastname: Fleet
authors.forename: H J
authors.initials: HJ
authors.lastname: Phaff
mesh_terms: D008841:Actin Cytoskeleton / Q000648:ultrastructure; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure*; D043326:Glucan 1,3-beta-Glucosidase; D005936:Glucans / Q000032:analysis*; D006026:Glycoside Hydrolases / Q000378:metabolism; D008854:Microscopy, Electron; D012568:Schizosaccharomyces / Q000737:chemistry / Q000648:ultrastructure*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D005936:Glucans; D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase; C020105:endo-1,6-beta-glucanase; C021780:exo-1,3-alpha-glucanase