title: Tumor suppressor gene p53 mutations in human prostate cancer.
abstract: The genetic background underlying the growth and development of human prostatic cancer is not yet clear. Here we searched for possible mutations in the entire coding region of tumor suppressor gene p53 in primary human prostatic carcinomas, using polymerase chain reaction and single-strand conformational polymorphism analysis of RNA. We found p53 gene mutations in 4 of 21 cases (19%). DNA sequencing of the polymerase chain reaction products revealed missense point mutations that resulted in amino acid changes in exon 5 or 3 in three cases and single base deletions in exon 7 in two cases. One case contained both a missense point mutation and a single base deletion. Three of these four cases were pathologically diagnosed as poorly differentiated adenocarcinomas, and three of the four cases were clinically localized to stage C or D. None of seven noncancerous prostate tissues nor three well-differentiated adenocarcinoma tissues showed any mutations. The present results suggest that p53 gene mutation is involved in the late progression steps of human prostate carcinogenesis.
authors.affiliation: Department of Urology, Yokohama City University, School of Medicine, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kubota
authors.forename: T
authors.initials: T
authors.lastname: Shuin
authors.forename: H
authors.initials: H
authors.lastname: Uemura
authors.forename: K
authors.initials: K
authors.lastname: Fujinami
authors.forename: H
authors.initials: H
authors.lastname: Miyamoto
authors.forename: S
authors.initials: S
authors.lastname: Torigoe
authors.forename: Y
authors.initials: Y
authors.lastname: Dobashi
authors.forename: H
authors.initials: H
authors.lastname: Kitamura
authors.forename: Y
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: K
authors.initials: K
authors.lastname: Danenberg
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D001483:Base Sequence; D004273:DNA, Neoplasm / Q000032:analysis; D005091:Exons; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D012334:RNA, Neoplasm / Q000032:analysis
chemical_list: D004273:DNA, Neoplasm; D012334:RNA, Neoplasm