Mechanism of interferon action: double-stranded RNA-specific adenosine deaminase from human cells is inducible by alpha and gamma interferons.
Treatment of human amnion U cells with interferon increased the steady state level of mRNA encoding the double-stranded (ds) RNA-specific adenosine deaminase (AdD) as measured by Northern gel-blot analysis. A single major dsRNA-specific AdD transcript of approximately 6.7 kb was detected; the transcript was induced by both interferon-alpha (IFN-alpha) and interferon-gamma (IFN-gamma). Likewise, Western immunoblot analysis revealed that a 150-kDa protein recognized by antiserum prepared against recombinant dsRNA-specific AdD was increased in the human amnion U and neuroblastoma SH-SY5Y cell lines treated with interferon. Both IFN-alpha and IFN-gamma induced the 150-kDa protein. These results, which establish that dsRNA-specific AdD is an IFN-inducible protein in human cells, have implications regarding the possible role of interferon in persistent viral infections.
Patterson
J B
JB
Department of Biological Sciences, University of California, Santa Barbara 93106, USA.
Thomis
D C
DC
Hans
S L
SL
Samuel
C E
CE
D000243:Adenosine Deaminase / Q000096:biosynthesis / Q000235:genetics*; D000650:Amnion / Q000166:cytology / Q000201:enzymology; D002478:Cells, Cultured; D018076:DNA, Complementary; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D011994:Recombinant Proteins; D014158:Transcription, Genetic / Q000187:drug effects; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D016898:Interferon-alpha; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D007371:Interferon-gamma; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase