title: Modulation of a recombinant glycine transporter (GLYT1b) by activation of protein kinase C.
abstract: Treatment of human embryonic kidney cells (HEK 293 cells) expressing the mouse glycine transporter 1 (GLYT1b) with the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) decreased specific [3H]glycine uptake. This down-regulation resulted from a reduction of the maximal transport rate and was blocked by the PKC inhibitors 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7) and staurosporine. The inhibitory effect of PMA treatment was also observed after removing all five predicted phosphorylation sites for PKC in GLYT1b by site-directed mutagenesis. These data indicate that glycine transport by GLYT1b is modulated by PKC activation; however, this regulation may involve indirect phosphorylation mechanisms.
authors.affiliation: Abteilung Neurochemie, Max-Planck-Institut für Hirnforschung, Frankfurt, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: R
authors.initials: R
authors.lastname: Adams
authors.forename: H
authors.initials: H
authors.lastname: Betz
authors.forename: P
authors.initials: P
authors.lastname: Schloss
mesh_terms: D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins / Q000378:metabolism*; D002460:Cell Line; D004789:Enzyme Activation; D005998:Glycine / Q000378:metabolism; D050487:Glycine Plasma Membrane Transport Proteins; D006801:Humans; D008564:Membrane Potentials; D009154:Mutation; D011493:Protein Kinase C / Q000378:metabolism*; D011994:Recombinant Proteins; D017923:Sodium-Hydrogen Exchangers / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; D050487:Glycine Plasma Membrane Transport Proteins; D011994:Recombinant Proteins; C493268:SLC6A9 protein, human; D017923:Sodium-Hydrogen Exchangers; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D005998:Glycine