title: Inhibition of cell cycle progression by sodium butyrate in normal rat kidney fibroblasts is altered by expression of the adenovirus 5 early 1A gene.
abstract: The effect of sodium butyrate (NaBut) on cell growth was studied in normal rat kidney (NRK) fibroblasts, and in NRK cells stably transfected with either the adenoviral gene E1A (wild-type), or mutated E1A (E1Amut; with a deletion in the CR1 domain), or with the transforming Ha-ras (EJ) gene. The growth of all these cell lines was inhibited by milimolar concentrations of sodium butyrate (NaBut). However, whereas the NRK cells as well as the NRK-E1Amut and NRK-ras cells were arrested in the G1 phase of the cell cycle, the NRK-E1A cells progressively accumulated in the G2 phase, suggesting that the E1A gene expression caused a "leaky" inhibition of G1 phase progression. The expression of late cell cycle-related genes cdc2 and PCNA (proliferating cell nuclear antigen) was not affected by NaBut in the NRK-E1A cells while it was totally suppressed in the other NRK-derived cell lines.
authors.affiliation: Helsinki University Hospital, Department of Oncology, Finland.
authors.forename: T
authors.initials: T
authors.lastname: Joensuu
authors.forename: J
authors.initials: J
authors.lastname: Mester
mesh_terms: D017500:Adenovirus E1A Proteins / Q000235:genetics* / Q000502:physiology; D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D016203:CDC2 Protein Kinase / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects*; D002461:Cell Line, Transformed; D005347:Fibroblasts / Q000166:cytology; D015870:Gene Expression; D006801:Humans; D007668:Kidney / Q000166:cytology; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D017500:Adenovirus E1A Proteins; D002087:Butyrates; D015513:Oncogene Proteins; D018809:Proliferating Cell Nuclear Antigen; C077183:human oncogene protein EJ-ras; D020148:Butyric Acid; D016203:CDC2 Protein Kinase