title: [Establishment and characterization of strain KE-39 derived from human gastric cancer].
abstract: We succeeded in establishing a human gastric carcinoma cell line (KE-39) from oncocytes obtained from the primary focus of a 77-year-old male stomach cancer patient. From a histopathological point of view the gastric carcinoma was a poorly differentiated adenocarcinoma exhibiting a funicular from and a structure with a solid vesicular focus. The oncocytes adhered to glass and proliferated in cell clusters, with a doubling time of about 38.4 hours. Upon transplantation of the cancer cells into nude mice, no visible tumors were found, but from a histological point of view poorly differentiated adenocarcinoma similar to the primary focus was found in all cases. With immunological staining they were found to be positive for anti-CEA antibody and anti-CA19-9 antibody, but negative for anti-ICAM-1 antibody. KE-39 is a cell line which was established from the primary focus, and it was reported in the belief that it is a useful cell line, upon the investigation of its cancer metastasis mechanism and cytological characteristics.
authors.affiliation: Division of Surgical Pathology, East Hospital Kitasato University.
authors.forename: H
authors.initials: H
authors.lastname: Uesugi
authors.forename: K
authors.initials: K
authors.lastname: Yamashita
authors.forename: E
authors.initials: E
authors.lastname: Atari
authors.forename: H
authors.initials: H
authors.lastname: Shinoda
authors.forename: T
authors.initials: T
authors.lastname: Nishiyama
authors.forename: I
authors.initials: I
authors.lastname: Okayasu
authors.forename: S
authors.initials: S
authors.lastname: Yasuda
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D018395:CA-19-9 Antigen / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000032:analysis; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D013274:Stomach Neoplasms / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D018395:CA-19-9 Antigen; D002272:Carcinoembryonic Antigen