Cloning and characterization of the glucokinase gene of Brucella abortus 19 and identification of three other genes.
A clone from Brucella abortus 19 complemented an Escherichia coli strain deficient in phosphorylation of glucose. Open reading frames similar to E. coli mepA, glk, and genes encoding ATP-coupled exporters were found in the sequence. A fourth affected growth on minimal media of the ptsI glk strain with various carbon sources.
Essenberg
R C
RC
Department of Biochemistry, Oklahoma State University, Stillwater 74078, USA.
D000595:Amino Acid Sequence; D001483:Base Sequence; D002003:Brucella abortus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D002352:Carrier Proteins / Q000235:genetics; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005941:Glucokinase / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping
D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D005941:Glucokinase; D010450:Endopeptidases; C021813:mepA protein, E coli