Negative-ion fast atom bombardment tandem mass spectrometry for characterization of sulfated unsaturated disaccharides from heparin and heparan sulfate.
Negative-ion fast atom bombardment tandem mass spectrometry has been used in the characterization of non-, mono-, di- and trisulfated disaccharides from heparin and heparan sulfate. The positional isomers of the sulfate group of monosulfated disaccharides were distinguished from each other by negative-ion fast atom bombardment tandem mass spectra, which provide an easy way of identifying the positional isomers. This fast atom bombardment collision induced dissociation mass spectrometry/mass spectrometry technique was also applied successfully to the characterization of di- and trisulfated disaccharides.
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Research and Development Laboratories, Soda Aromatic Co., Ltd, Chiba, Japan.
Kubota
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Okuda
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Hirano
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Ohashi
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D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000032:analysis* / Q000737:chemistry; D006493:Heparin / Q000737:chemistry*; D006497:Heparan Sulfate / Q000737:chemistry*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D016339:Spectrometry, Mass, Fast Atom Bombardment / Q000379:methods; D013431:Sulfates / Q000032:analysis* / Q000737:chemistry
D004187:Disaccharides; D006493:Heparin; D006497:Heparan Sulfate; D013431:Sulfates