title: Differentiation of pathogenic Escherichia coli strains in Brazilian children by PCR.
abstract: A PCR technique to differentiate pathogenic enteric Escherichia coli strains in a field setting was evaluated. Among 76 children with acute diarrhea, this technique identified 12 children (16%) with enterotoxigenic E. coli, 6 (8%) with enteropathogenic E. coli, and 1 (1%) with enteroinvasive E. coli infection. Compared with the conventional assays, the PCR method proved to be simpler, more rapid, and inexpensive and therefore suitable for application in a developing-country field setting.
authors.affiliation: Department of Medicine, Universidade Federal da Bahia, Salvador de Bahia, Brazil.
authors.forename: N G
authors.initials: NG
authors.lastname: Tornieporth
authors.forename: J
authors.initials: J
authors.lastname: John
authors.forename: K
authors.initials: K
authors.lastname: Salgado
authors.forename: P
authors.initials: P
authors.lastname: de Jesus
authors.forename: E
authors.initials: E
authors.lastname: Latham
authors.forename: M C
authors.initials: MC
authors.lastname: Melo
authors.forename: S T
authors.initials: ST
authors.lastname: Gunzburg
authors.forename: L W
authors.initials: LW
authors.lastname: Riley
mesh_terms: D001431:Bacteriological Techniques; D001483:Base Sequence; D001938:Brazil / Q000453:epidemiology; D002675:Child, Preschool; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D003967:Diarrhea / Q000453:epidemiology / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology; D005069:Evaluation Studies as Topic; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial