Fluorometric detection of glycosphingolipids on thin-layer chromatographic plates.
A microdetection system for glycosphingolipid analysis has been developed using 5-hydroxy-1-tetralone as the fluorescent labeling reagent. The reagents in H2SO4 permit the fluorometric detection of acidic and neutral glycosphingolipids both in test tube and on thin-layer chromatographic plates. Glycosphingolipids can be detected at concentrations as low as 5 pmol on the thin-layer chromatographic plate. The method is a rapid and simple, and feasible for determination of glycosphingolipid from small amounts of biological samples.
Watanabe
K
K
Division of Biochemistry, Shigei Medical Research Institute, Okayama, Japan.
Mizuta
M
M
D000818:Animals; D002855:Chromatography, Thin Layer; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods*; D006028:Glycosphingolipids / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D009284:Naphthols; D051381:Rats; D012788:Shrews; D013050:Spectrometry, Fluorescence; D044024:Tetralones
D005456:Fluorescent Dyes; D006028:Glycosphingolipids; D009284:Naphthols; D044024:Tetralones; C001428:5-hydroxy-1-tetralone