Regulation of cell surface GLUT1, GLUT3, and GLUT4 by insulin and IGF-I in L6 myotubes.
The effects of insulin and IGF-I on the cell surface quantities of GLUT1, GLUT3 and GLUT4 glucose transporters in L6 myotubes were determined with the exofacial bis-mannose phololabel (ATB-BMPA). In basal cells, an equal molar quantity of each transporter isoform was found at the cell surface. Insulin stimulated the translocation of all three glucose transporter isoforms to the plasma membrane fraction from the light microsome fraction, resulting in equal molar quantities on the cell surface. IGF-I stimulated a similar translocation of all isoforms, augmented by an increase in surface GLUT3 as assessed by ATB-BMPA.
Wilson
C M
CM
Experimental Diabetes, Metabolism and Nutrition Section, NIDDK National Institutes of Health, Bethesda, MD 20892-1420, USA.
Mitsumoto
Y
Y
Maher
F
F
Klip
A
A
D000345:Affinity Labels; D000818:Animals; D001386:Azides; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004187:Disaccharides; D005947:Glucose / Q000378:metabolism*; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D051275:Glucose Transporter Type 4; D006027:Glycosides; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D051379:Mice; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D009124:Muscle Proteins; D018482:Muscle, Skeletal / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D011437:Propylamines
D000345:Affinity Labels; D001386:Azides; D004187:Disaccharides; D051272:Glucose Transporter Type 1; D051274:Glucose Transporter Type 3; D051275:Glucose Transporter Type 4; D006027:Glycosides; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D011437:Propylamines; C495677:SLC2A1 protein, human; C495687:SLC2A3 protein, human; C495692:SLC2A4 protein, human; C495678:Slc2a1 protein, mouse; C495689:Slc2a3 protein, mouse; C495693:Slc2a4 protein, mouse; C065412:2-N-(4-(1-azitrifluoroethyl)benzoyl)-1,3-bis-(mannos-4-yloxy)-2-propylamine; D007334:Insulin-Like Growth Factor I; D005947:Glucose