Interaction of filarial proteins on growth regulation of normal lung epithelial cells in vitro.
An in vitro model to examine the effects of filarial proteins on lung epithelial cells has been developed. Several of these proteins appear in circulation of infected individuals. A close association between tropical pulmonary eosinophilia (TPE) and filariasis has been reported by several workers. [3H]-thymidine studies do indicate that when optimum concentration of these filarial proteins were added to lung cultures in proliferating and basal/maintenance media a further increase in growth stimulation was observed early in culture. However, on longer exposures and at higher concentrations an inhibitory effect with distinct morphological changes were noted. The dual role of these proteins on lung epithelial cells in vitro may highlight the possibility of a direct interaction of these proteins with lung cells during disease also contributing to tissue damage.
Maya
A
A
Centre for Biotechnology, Anna University, Madras, India.
Usha
S
S
Jayaraman
K
K
Baba
K
K
Sukumaran
M
M
Balakrishnan
A
A
D000818:Animals; D017178:Brugia malayi / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured / Q000469:parasitology; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004848:Epithelium / Q000469:parasitology; D015801:Helminth Proteins / Q000009:adverse effects*; D006790:Host-Parasite Interactions / Q000502:physiology; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology* / Q000469:parasitology; D008174:Lung Diseases, Parasitic / Q000469:parasitology / Q000503:physiopathology; D008842:Microfilariae / Q000737:chemistry; D011657:Pulmonary Eosinophilia / Q000469:parasitology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D017182:Setaria Nematode / Q000502:physiology; D013997:Time Factors
D003470:Culture Media; D015801:Helminth Proteins