Association of DQB1*0302 alloantigens in Japanese pediatric patients with steroid-sensitive nephrotic syndrome.
We identified human leukocyte alloantigens (HLA) class II alleles in 24 Japanese children with steroid-sensitive nephrotic syndrome (SSNS) by deoxyribonucleic acid (DNA) typing. The DQA1 and DQB1 alleles were identified using sequence-specific oligonucleotide probes for DQA and DQB. The frequency of DQB1*0302 was significantly higher in the patients than in the controls (54.0 vs. 16.0%, respectively; relative risk, RR = 6.2; pc < 0.00009. We also found that the frequency of DQA1*0103 in the patients was significantly lower than in the controls (RR = 0.194, pc < 0.04). Several studies have identified an association between certain HLA by serotyping. In the present study, we investigated the HLAs of Japanese patients with SSNS by DNA typing and observed a significant increase in the frequency of DQB1*0302 in patients with the disease. HLA-DQ3, which was proven to be associated with SSNS, consists of HLA DQ7, 8 and 9. DQB1*0302 is a component of HLA-DQ8. So we proposed the increase of DQ3 was due to an increase in DQ8.
Abe
K K
KK
Second Department of Internal Medicine, Fukuoka University School of Medicine, Japan.
Michinaga
I
I
Hiratsuka
T
T
Ogahara
S
S
Naito
S
S
Arakawa
K
K
Tsuru
N
N
Tokieda
K
K
D000293:Adolescent; D000483:Alleles; D001483:Base Sequence; D002648:Child; D004247:DNA / Q000235:genetics; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D009404:Nephrotic Syndrome / Q000188:drug therapy / Q000453:epidemiology / Q000235:genetics / Q000276:immunology*; D013256:Steroids / Q000627:therapeutic use*
D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; D059866:HLA-DQ beta-Chains; C069050:HLA-DQA1 antigen; C069051:HLA-DQB1 antigen; D013256:Steroids; D004247:DNA