Cell cycle-dependent expression of Ki-67 antigen in human melanoma cells subjected to irradiation and/or hyperthermia.
The proliferation of human melanoma cells in vitro during the first 3 days after irradiation and/or hyperthermia was followed by two-parameter flow cytometry combining cell cycle analysis on the basis of DNA content with Ki-67 antibody labeling. It was found that cells arrested or delayed in the S and G2 phases of the cell cycle were Ki-67-positive in spite of the antigen's very short half-life. Thus Ki-67 staining failed to reflect those changes in cell proliferation which typically occur in the course of a fractionated radiotherapy as well as those expected in the case of hyperthermia or a combined treatment.
Zölzer
F
F
Institut für Medizinische Strahlenbiologie, Universitätsklinikum Essen, Germany.
Streffer
C
C
D002453:Cell Cycle / Q000528:radiation effects*; D005434:Flow Cytometry; D006358:Hot Temperature; D006801:Humans; D019394:Ki-67 Antigen; D008545:Melanoma / Q000276:immunology* / Q000473:pathology; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009687:Nuclear Proteins / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins