title: Guanosine 3',5'-bispyrophosphate (ppGpp) synthesis in cells of Escherichia coli starved for Pi.
abstract: Cells of Escherichia coli which enter a phase of starvation for Pi induce the synthesis of the nucleotide guanosine 3',5'-bispyrophosphate (ppGpp). This induction is relA independent but depends on the spoT gene product. A mutant unable to produce ppGpp is impaired in the expression of two genes which belong to the pho regulon, a defect which is dependent on the product of spoT. We suggest that ppGpp is essential for the proper induction of the genes which belong to the pho regulon.
authors.affiliation: Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Israel.
authors.forename: B
authors.initials: B
authors.lastname: Spira
authors.forename: N
authors.initials: N
authors.lastname: Silberstein
authors.forename: E
authors.initials: E
authors.lastname: Yagil
mesh_terms: D000469:Alkaline Phosphatase / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D006159:Guanosine Tetraphosphate / Q000096:biosynthesis*; D010710:Phosphates / Q000172:deficiency*; D011755:Pyrophosphatases / Q000096:biosynthesis; D018085:Regulon / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D010710:Phosphates; C061278:PhoB protein, Bacteria; D006159:Guanosine Tetraphosphate; D000469:Alkaline Phosphatase; C027627:guanosine-3',5'-bis(diphosphate) 3'-pyrophosphatase; D011755:Pyrophosphatases