Enhancing protein solubilization with nondetergent sulfobetaines.
A significant increase in the yield of protein extraction by the addition of sulfobetaine-type mild solubilization agents is shown for microsomal membrane proteins and proteins from lyophilized platelets, with a maximum increase in yield of up to 100%. These agents are also applied to native isoelectric focusing in immobilized pH gradients. The absence of significant denaturation induced by these solubilizers is shown for creatine kinase.
Vuillard
L
L
Institute Laue-Langevin, Grenoble, France.
Marret
N
N
Rabilloud
T
T
D000818:Animals; D001622:Betaine / Q000031:analogs & derivatives*; D001792:Blood Platelets / Q000737:chemistry; D001798:Blood Proteins / Q000302:isolation & purification; D003402:Creatine Kinase / Q000302:isolation & purification; D005612:Freeze Drying; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007425:Intracellular Membranes / Q000737:chemistry; D007525:Isoelectric Focusing / Q000379:methods; D008565:Membrane Proteins / Q000302:isolation & purification; D051379:Mice; D008810:Mice, Inbred C57BL; D008861:Microsomes / Q000737:chemistry; D009132:Muscles / Q000201:enzymology; D011506:Proteins / Q000302:isolation & purification*; D011817:Rabbits; D012995:Solubility; D013552:Swine
D001798:Blood Proteins; D007202:Indicators and Reagents; D008565:Membrane Proteins; D011506:Proteins; D001622:Betaine; D003402:Creatine Kinase