title: Specific variants of H1 histone regulate CpG methylation in eukaryotic DNA.
abstract: Upon HPLC fractionation of human placenta or calf thymus H1 histone preparations, only some fractions enriched in the H1e-c variants were able to exert a severe inhibition on in vitro enzymatic DNA methylation. These fractions, though similar to the other variants in interacting with genomic DNA, were also the only ones which could bind CpG-rich ds-oligodeoxyribonucleotides (oligos). Both the 6-CpG ds-oligo and the DNA purified from chromatin fractions enriched in 'CpG islands' were good competitors for the binding of H1e-c to the 6meCpG ds-oligo. This ability to bind any DNA sequence and to suppress the enzymatic methylation in any sequence containing CpG dinucleotides suggests, for these particular H1 variants, a possible role in maintaining CpG island DNA and linker DNA at low methylation levels.
authors.affiliation: Department of Human Biopathology, University of Rome, La Sapienza, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Strom
authors.forename: R
authors.initials: R
authors.lastname: Santoro
authors.forename: M
authors.initials: M
authors.lastname: D'Erme
authors.forename: S
authors.initials: S
authors.lastname: Mastrantonio
authors.forename: A
authors.initials: A
authors.lastname: Reale
authors.forename: S
authors.initials: S
authors.lastname: Marenzi
authors.forename: G
authors.initials: G
authors.lastname: Zardo
authors.forename: P
authors.initials: P
authors.lastname: Caiafa
mesh_terms: D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002843:Chromatin / Q000737:chemistry / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D015226:Dinucleoside Phosphates; D005260:Female; D014644:Genetic Variation; D006657:Histones / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008745:Methylation; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D013950:Thymus Gland / Q000378:metabolism
chemical_list: D002843:Chromatin; D004247:DNA; D015226:Dinucleoside Phosphates; D006657:Histones; D009838:Oligodeoxyribonucleotides; C015772:CpG dinucleotide