Human embryonic zeta-globin gene expression in mouse-human hybrid erythroid cell lines.
The human alpha-globin-like embryonic zeta-globin chains are present in abundance during the first 5 to 6 weeks of gestation. Subsequently, zeta-globin chains are present in fetal blood at a very low level, which is supplanted by the expression of alpha-globin chains. Adult individuals who are carriers of the (--SEA/) alpha-thalassemia deletion, in contrast to normal adults, have low levels of embryonic zeta-globin chains in their circulating erythrocytes. In this investigation, we constructed stable mouse-human hybrid cells with murine erythroleukemia cells bearing human chromosome 16, with either the normal alpha-globin gene cluster (alpha alpha/) or the (--SEA/) type of alpha-thalassemia deletion. The results on the human zeta-globin gene expression in these hybrid cells indicate that murine adult erythroid transcription factors can induce the expression of human embryonic zeta-globin gene is cis to the (--SEA/) deletion, in parallel with the endogenous mouse alpha-globin gene expression. These data also show the importance of the DNA sequences within the (--SEA) deletion in regulating the expression of zeta-globin gene in cis during normal human hemoglobin ontogeny.
Luo
H Y
HY
Department of Pathology, McMaster University School of Medicine, Hamilton, Ontario, Canada.
Deisseroth
A B
AB
Chui
D H
DH
D000818:Animals; D015672:Erythroid Precursor Cells / Q000502:physiology*; D015870:Gene Expression; D005914:Globins / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid; D017384:Sequence Deletion
D012333:RNA, Messenger; D005914:Globins