title: Complete genomic sequence of the fish rhabdovirus infectious haematopoietic necrosis virus.
abstract: The complete nucleotide sequence of the genome of the fish rhabdovirus infectious haematopoietic necrosis virus (IHNV) has been determined after cDNA cloning of the viral genomic RNA. Sequence analysis showed the presence of six open reading frames encoding the nucleoprotein N, the matrix proteins M1 and M2, the glycoprotein G, a so-called non-structural protein NV, and the RNA polymerase L. The genome organization is 3'N-M1-M2-G-NV-L 5'. The extreme 5' and 3' ends of the genome were sequenced after RNA ligation or RACE. Prokaryotic expression products of the open reading frames predicted to encode the matrix proteins M1 and M2, the glycoprotein G and the NV protein reacted with rabbit anti-IHNV serum thereby confirming their identity. This is the first complete nucleotide sequence of a fish rhabdovirus. Knowledge of the complete sequence is an essential prerequisite for future manipulation of the genome and also serves to provide gene- and protein specific reagents for use in further examination of the replication of the fish rhabdoviruses.
authors.affiliation: Federal Research Centre for Virus Diseases of Animals, Institute of Molecular and Cellular Virology, Friedrich-Loeffler-Institutes, Insel-Riems, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Schütze
authors.forename: P J
authors.initials: PJ
authors.lastname: Enzmann
authors.forename: R
authors.initials: R
authors.lastname: Kuchling
authors.forename: E
authors.initials: E
authors.lastname: Mundt
authors.forename: H
authors.initials: H
authors.lastname: Niemann
authors.forename: T C
authors.initials: TC
authors.lastname: Mettenleiter
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral; D001483:Base Sequence; D003001:Cloning, Molecular; D016679:Genome, Viral; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000821:virology; D016366:Open Reading Frames / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012209:Rhabdoviridae / Q000235:genetics* / Q000276:immunology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000032:analysis / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins