Vitamin C activity of erythorbic acid in ascorbic acid-deficient guinea pigs.
The vitamin C activity of erythorbic acid (ErA) in ascorbic acid (AsA)-deficient guinea pigs was evaluated. The guinea pigs depleted AsA for 16 days were divided into two groups: one group (control group) was supplemented with 1, 5, or 100 mg/day AsA and the other group (experimental group) was supplemented with 1, 5, 20, or 100 mg/day ErA for 4 days. The contents of AsA and ErA in the tissues of guinea pigs were determined by using HPLC, and the activities of liver aniline hydroxylase, of serum alkaline phosphatase and the content of liver cytochrome P-450 were measured. The AsA tissue content of AsA-supplemented guinea pigs was much higher than the ErA tissue content of ErA-supplemented ones, and also, the activities of liver aniline hydroxylase, of serum alkaline phosphatase and the content of liver cytochrome P-450 of AsA-supplemented animals were much higher than those of ErA-supplemented animals, even when the supplemented amount of ErA was equal to that of AsA. Based on these results, the vitamin C activity of ErA seems to be much lower than that of AsA in the AsA-deficient guinea pigs. This suggested that the apparent vitamin C activity of ErA was dependent on the AsA tissue levels of guinea pigs.
Suzuki
E
E
Department of Human Biological Studies, Ochanomizu University, Tokyo, Japan.
Kurata
T
T
Tomita
M
M
Arakawa
N
N
D000311:Adrenal Glands / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000097:blood; D000815:Aniline Hydroxylase / Q000032:analysis; D000818:Animals; D001205:Ascorbic Acid / Q000493:pharmacokinetics / Q000502:physiology / Q000627:therapeutic use*; D001206:Ascorbic Acid Deficiency / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D006168:Guinea Pigs; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008297:Male; D013237:Stereoisomerism
C005056:isoascorbic acid; D003577:Cytochrome P-450 Enzyme System; D000815:Aniline Hydroxylase; D000469:Alkaline Phosphatase; D001205:Ascorbic Acid