Reduced virulence of Candida albicans PHR1 mutants.
Candida albicans mutants lacking PHR1 exhibit a pH-dependent morphogenic defect which is expressed at pH 7.5, a pH comparable to that of mammalian blood (S. M. Saporito-Irwin, C. E. Birse, P. S. Sypherd, and W. A. Fonzi, Mol. Cell. Biol. 15:601-613, 1995). The in vivo relevance of this expression pattern was tested in a mouse model of systemic candidiasis. A phr1/phr1 mutant was found to be less virulent than an isogenic Phr1+ strain and exhibited altered morphological development in vivo. These results indicate that PHR1 contributes to the virulence of C. albicans.
Ghannoum
M A
MA
Division of Infectious Diseases, Harbor-UCLA Research and Education Institute, St. John's Cardiovascular Research Center, Torrance 90509, USA.
Spellberg
B
B
Saporito-Irwin
S M
SM
Fonzi
W A
WA
D000818:Animals; D001051:Apoenzymes; D002176:Candida albicans / Q000235:genetics / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology*; D004255:Deoxyribodipyrimidine Photo-Lyase; D005656:Fungal Proteins / Q000502:physiology*; D005800:Genes, Fungal; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000382:microbiology; D008562:Membrane Glycoproteins; D051379:Mice; D008807:Mice, Inbred BALB C; D009024:Morphogenesis
D001051:Apoenzymes; D005656:Fungal Proteins; D008562:Membrane Glycoproteins; C091515:PHR1 protein, Candida albicans; D004255:Deoxyribodipyrimidine Photo-Lyase