Efficient production of syngeneic anti-IgE monoclonal antibodies with high affinity and diverse specificity.
Syngeneic monoclonal anti-IgE antibodies are of value in studies of the suppression of IgE synthesis. Procedures are described here for the production of high titers of murine anti-IgE antibodies by initiating immunization in the perinatal period, before mice develop tolerance to their autologous IgE. This in turn facilitates the production of monoclonal anti-IgE antibodies. Properties of some of these mAbs are reported, including affinity, fine specificity and ability to bind to IgE on B lymphoma cells or mast cells.
Haba
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Rosenstiel Research Center, Department of Biology, Brandeis University, Waltham, MA 02254, USA.
Nisonoff
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D000707:Anaphylaxis / Q000209:etiology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000915:Antibody Affinity; D000918:Antibody Specificity; D015550:Cell Degranulation; D002460:Cell Line; D006825:Hybridomas; D007132:Immunoglobulin Isotypes / Q000276:immunology; D016393:Lymphoma, B-Cell / Q000276:immunology; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D017455:Receptors, IgE / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007132:Immunoglobulin Isotypes; D017455:Receptors, IgE; C026408:anti-IgE antibodies