Site-specific reactivities of cysteine residues in horse L-apoferritin.
Recombinant horse L-apoferritin and its mutants were used to compare the reactivities of two different cysteinyl residues with 7-fluoro-4-sulfamoyl-2,1,3-benzoxadiazole (ABD-F), p-chloromercuribenzoic acid (PCMB), N-(9-acridinyl)maleimide (NAM), and 4-maleimido-2,2,6,6-tetramethylpiperidine-N-oxyl (NEM-TEMPO). ABD-F selectively reacted with cysteine 52 (C52), which is located on the inner surface of the peptide shell of apoferritin. In contrast, PCMB reacted only with cysteine 130 (C130), which is located at the 3-fold channels of the shell. NAM and NEM-TEMPO reacted with both C52 and C130.
Takeda
S
S
Nagayama Protein Array Project, ERATO, JRDC, Ibaraki.
Yamaki
M
M
Ebina
S
S
Nagayama
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001052:Apoferritins / Q000737:chemistry* / Q000378:metabolism*; D001483:Base Sequence; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003497:Cyclic N-Oxides / Q000494:pharmacology; D003545:Cysteine; D017931:DNA Primers; D005456:Fluorescent Dyes; D006736:Horses; D007700:Kinetics; D008301:Maleimides / Q000494:pharmacology; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010069:Oxadiazoles / Q000494:pharmacology; D010449:Peptide Mapping; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D020245:p-Chloromercuribenzoic Acid
D002729:Chloromercuribenzoates; D003497:Cyclic N-Oxides; D017931:DNA Primers; D005456:Fluorescent Dyes; D008301:Maleimides; D010069:Oxadiazoles; D011994:Recombinant Proteins; C017480:4-maleimido-2,2,6,6-tetramethylpiperidinooxyl; C080490:N-(9-acridinyl)maleimide; D020245:p-Chloromercuribenzoic Acid; D001052:Apoferritins; C046364:4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole; D003545:Cysteine