Cloning and characterization of the human invasion suppressor gene E-cadherin (CDH1).
E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. By using recombinant lambda phage, cosmid, and P1 phage clones, we isolated the full-length human E-cadherin gene (CDH1). The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1 was confirmed by FISH analysis. Detailed restriction mapping and partial sequence analysis of the gene allowed us to identify 16 exons and a 65-kb-long intron 2. The intron-exon boundaries are highly conserved in comparison with other "classical cadherins." In intron 1 we identified a 5' high-density CpG island that may be implicated in transcription regulation during embryogenesis and malignancy.
Berx
G
G
Section Molecular Cell Biology, University of Ghent, Belgium.
Staes
K
K
van Hengel
J
J
Molemans
F
F
Bussemakers
M J
MJ
van Bokhoven
A
A
van Roy
F
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D015820:Cadherins / Q000235:genetics*; D002448:Cell Adhesion; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16; D003001:Cloning, Molecular; D004742:Enhancer Elements, Genetic; D005091:Exons; D005786:Gene Expression Regulation; D005796:Genes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D015820:Cadherins