Catabolism of indole-3-acetic acid and 4- and 5-chloroindole-3-acetic acid in Bradyrhizobium japonicum.
Some strains of Bradyrhizobium japonicum have the ability to catabolize indole-3-acetic acid. Indoleacetic acid (IAA), 4-chloro-IAA (4-Cl-IAA), and 5-Cl-IAA were metabolized to different extents by strains 61A24 and 110. Metabolites were isolated and analyzed by high-performance liquid chromatography and conventional mass spectrometry (MS) methods, including MS-mass spectroscopy, UV spectroscopy, and high-performance liquid chromatography-MS. The identified products indicate a novel metabolic pathway in which IAA is metabolized via dioxindole-3-acetic acid, dioxindole, isatin, and 2-aminophenyl glyoxylic acid (isatinic acid) to anthranilic acid, which is further metabolized. Degradation of 4-Cl-IAA apparently stops at the 4-Cl-dioxindole step in contrast to 5-Cl-IAA which is metabolized to 5-Cl-anthranilic acid.
Jensen
J B
JB
Department of Molecular Biology, University of Aarhus, Denmark.
Egsgaard
H
H
Van Onckelen
H
H
Jochimsen
B U
BU
D002851:Chromatography, High Pressure Liquid; D007210:Indoleacetic Acids / Q000378:metabolism*; D007510:Isatin / Q000378:metabolism; D013058:Mass Spectrometry; D008954:Models, Biological; D010937:Plant Growth Regulators / Q000378:metabolism*; D012230:Rhizobiaceae / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D007210:Indoleacetic Acids; D010937:Plant Growth Regulators; C096199:5-chloroindole-3-acetic acid; C096198:4-chloroindole-3-acetic acid; C030737:indoleacetic acid; D007510:Isatin