Analysis of overlapping cDNA clones specific for a putative second DNA methyltransferase-encoding gene in Arabidopsis thaliana.
We have isolated and sequenced overlapping cDNA clones specific for a putative second DNA methyltransferase (MTase)-encoding gene (MTase11) from Arabidopsis thaliana (At) recently described as a genomic DNA fragment [Scheidt et al., Nucleic Acids Res. 22 (1994) 953-958]. The gene seems to be present as a single copy in the At genome and is transcribed into a 2.2-kb messenger detectable only in young seedlings. Using sequence comparison we found structural differences between the cDNA clones and the previously reported genomic fragment. The amino-acid sequence deduced from the 1.8-kb cDNA sequence shows the occurrence of the conserved motif number VI characteristic for m5C-MTases. Northern and Southern analysis detects no cross-hybridization with the originally described MTase-encoding At gene (MTase1) [Finnegan and Dennis, Nucleic Acids Res. 21 (1993) 2383-2388].
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Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
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D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D029681:Arabidopsis Proteins; D015152:Blotting, Northern; D015139:Blotting, Southern; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary; D017343:Genes, Plant; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis; D017386:Sequence Homology, Amino Acid
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D011994:Recombinant Proteins; C094512:MTase11 protein, Arabidopsis; D004248:DNA (Cytosine-5-)-Methyltransferases