A new method for spectrophotometric determination of thiomersal in biologicals.
A coloured complex formed with thiomersal by the action of dithizone, acetone, water and sodium hydroxide has been described and developed to quantitate even less than a microgram of thiomersal in biologicals. The method is simple, specific, easy to carry out, consumes little time and requires a few microlitres of sample. The results are reproducible.
Shrivastaw
K P
KP
Biochemistry Laboratory, Central Research Institute, Kasauli, India.
Singh
S
S
D000096:Acetone / Q000737:chemistry; D001688:Biological Products / Q000737:chemistry*; D004230:Dithizone / Q000737:chemistry; D015203:Reproducibility of Results; D012972:Sodium Hydroxide / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods*; D013849:Thimerosal / Q000032:analysis*; D014867:Water / Q000737:chemistry
D001688:Biological Products; D014867:Water; D000096:Acetone; D013849:Thimerosal; D012972:Sodium Hydroxide; D004230:Dithizone