title: Isolation and primary structure determination of a metallothionein from Paracentrotus lividus (Echinodermata, Echinoidea).
abstract: A low-molecular-mass zinc-binding protein was purified from the eggs of the sea urchin Paracentrotus lividus using procedures that included gel-permeation and anion-exchange chromatography followed by HPLC. The primary structure of this protein was derived from the sequences of peptide fragments obtained by digestion with trypsin and thermolysin. The reconstructed sequence showed the presence of 20 cysteinyl residues, thus resembling that of a metallothionein. The Paracentrotus protein was most similar to the metallothionein of Strongylocentrotus purpuratus, another member of the order of Echinoida, living along the coast of the Pacific Ocean. However, the presence of non-conservative amino acid substitution, together with a deletion of two residues in the Strongylocentrotus metallothionein, make the similarity scores of the two sea urchin proteins lower than that of metallothioneins from vertebrates of the same order. In addition, the present data show that sea urchin metallothioneins display no homology with metallothioneins of any other species.
authors.affiliation: Istituto di Biochimica delle Proteine ed Enzimologia, Consiglio Nazionale Delle Ricerche, Naples, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Scudiero
authors.forename: C
authors.initials: C
authors.lastname: Capasso
authors.forename: F
authors.initials: F
authors.lastname: Del Vecchio-Blanco
authors.forename: G
authors.initials: G
authors.lastname: Savino
authors.forename: A
authors.initials: A
authors.lastname: Capasso
authors.forename: A
authors.initials: A
authors.lastname: Parente
authors.forename: E
authors.initials: E
authors.lastname: Parisi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004447:Echinodermata / Q000737:chemistry*; D008668:Metallothionein / Q000737:chemistry / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D016415:Sequence Alignment; D013820:Thermolysin; D014357:Trypsin
chemical_list: D010446:Peptide Fragments; D008668:Metallothionein; D014357:Trypsin; D013820:Thermolysin