title: Fractionation and characterization of two forms of peroxidase from Oryza sativa.
abstract: Peroxidase (E.C. 1.11.1.7., hydrogen donor oxidoreductase) is widely distributed and has been isolated from many higher plants (1). The wide distribution of the enzyme suggests that it could be of great biological importance. However the role that it plays in metabolism is not clear due to the large number of reactions it catalyzes and the considerable number of isozymic species (2). In tomato plants, Evans and Aldridge (3) separated out six isoperoxidases and in a later paper Evans reported 12 isoperoxidases from tomato shoots (4). A homogeneous tomato fruit peroxidase isozyme was obtained by Jen et al. (5) using hydrophobic chromatography. Isozymes were not detected in Euphorbia characias peroxidase (6), in Ipomoea batatas peroxidase (7) and in Hordeum vulgare peroxidase (8). The simultaneous presence of Cu (II) amine oxidase and peroxidase in cell walls suggests that the peroxide generated on oxidation of the amines could be utilized by the peroxidase (6,8,9). In the graminea Oryza sativa, widely distributed, an FAD amine oxidase is present that oxidizes diamines (10). In this plant we also found two isoperoxidases called perox I and II. Only perox I was purified to homogeneity and its enzymatic, physical and chemical properties have been studied.
authors.affiliation: Istituto di Chimica Biologica, Università di Cagliari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Padiglia
authors.forename: R
authors.initials: R
authors.lastname: Medda
authors.forename: G
authors.initials: G
authors.lastname: Pazzaglia
authors.forename: A
authors.initials: A
authors.lastname: Rescigno
authors.forename: E
authors.initials: E
authors.lastname: Cruciani
authors.forename: G
authors.initials: G
authors.lastname: Floris
mesh_terms: D005591:Chemical Fractionation; D003962:Dianisidine / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D012275:Oryza / Q000201:enzymology*; D010544:Peroxidases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013053:Spectrophotometry; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D007527:Isoenzymes; D010544:Peroxidases; D003962:Dianisidine