[Hepatocellular transplantation in rats with congenital ascorbic acid deficiency].
The purpose of this study was to investigate suitable sites for hepatocyte transplantation in rats with congenital liver enzyme deficiency. Hepatocytes were isolated from ODS-(+)/+ rats, which are congenic to ODS-od/od rats and have hepatic L-gulonolactone oxidase. A total of 1 x 10(7), 1 x 10(7), and 2.5 x 10(6) hepatocytes were respectively transplanted into the peritoneal cavity, spleen, or portal vein of ODS-od/od rats, which are unable to synthesize ascorbic acid (AsA) due to lack of hepatic L-gulonolactone oxidase. After 4 days of oral pretreatment with 0.05% 2-acetylaminofluorene, recipients underwent 70% partial hepatectomy just before transplantation. AsA administration was discontinued at 6 weeks after transplantation. The symptom-free survival rate and the serum AsA level of recipient rats were determined at 6 weeks after discontinuing AsA administration. The symptom-free survival rate of untransplanted rats and recipient rats with intraperitoneal, intrasplenic and intraportal hepatocyte transplantation were 0%, 0%, 60%, and 100%, respectively. The serum AsA levels were 0.20 +/- 0.20 microgram/ml, 0.14 +/- 0.05 microgram/ml, 1.06 +/- 0.26 microgram/ml, and 1.58 +/- 0.61 microgram/ml, respectively. Intrasplenic or intraportal transplantation was able to cure ODS-od/od rats. A subsequent splenectomy study showed that hepatocytes reaching the liver via the splenic vein following intrasplenic hepatocyte transplantation played a major role in this experimental success.
Onodera
K
K
Second Department of Surgery, Asahikawa Medical College, Japan.
Kasai
S
S
Mito
M
M
D000818:Animals; D001205:Ascorbic Acid / Q000096:biosynthesis / Q000097:blood; D001206:Ascorbic Acid Deficiency / Q000151:congenital* / Q000378:metabolism / Q000601:surgery; D017690:Cell Transplantation; D050544:L-Gulonolactone Oxidase; D008099:Liver / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D010529:Peritoneal Cavity; D011169:Portal Vein; D051381:Rats; D011922:Rats, Mutant Strains; D013154:Spleen; D013401:Sugar Alcohol Dehydrogenases / Q000172:deficiency
D013401:Sugar Alcohol Dehydrogenases; D050544:L-Gulonolactone Oxidase; D001205:Ascorbic Acid