Interleukin-10 in human milk.
The concentrations of immunoreactive IL-10 in the aqueous fraction of 20 specimens of human milk obtained during the first 80 h of lactation and stored at -60 degrees C ranged from 66 to 9301 pg/mL (mean +/- SD, 3304 +/- 3127 pg/mL). IL-10 was present also in the lipid layer of milk. Gel filtration revealed that IL-10 was located in a high molecular weight fraction, where certain other cytokines in human milk have been found. In addition, immunoreactive IL-10 in milk increased after treatment with sodium taurocholate. Bioactive IL-10 was demonstrated by the finding that human milk inhibited [3H]thymidine uptake by human blood lymphocytes and that inhibition was partly overcome by concomitant incubation with antibodies to human IL-10. IL-10 mRNA but no protein product was found in cultured human mammary epithelial cells. Some IL-10 was associated with preparations of human milk leukocytes, but the data did not suggest that the cells were producing the cytokine. Bioactive IL-10 in a possible protected compartment suggests that IL-10 in human milk may have immunomodulating, antiinflammatory effects on the alimentary tract of the recipient infant.
Garofalo
R
R
University of Texas Medical Branch, Department of Pediatrics, Galveston 77555-0369, USA.
Chheda
S
S
Mei
F
F
Palkowetz
K H
KH
Rudloff
H E
HE
Schmalstieg
F C
FC
Rassin
D K
DK
Goldman
A S
AS
D000328:Adult; D001483:Base Sequence; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis*; D008895:Milk, Human / Q000737:chemistry*; D008969:Molecular Sequence Data; D013696:Temperature
D016207:Cytokines; D016753:Interleukin-10