Immunoassays for detecting cytokines: what are they really measuring?
In summary, many factors influence the estimates of cytokine levels provided by immunoassays. If immunoassays are to supply data which are valid and useful to researchers and clinicians, these factors must be fully investigated during assay design and construction.
Mire-Sluis
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AR
Division of Immunobiology, National institute for Biological Standards and Control, Potters Bar, Herts, UK.
Gaines-Das
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R
Thorpe
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R
D000818:Animals; D000918:Antibody Specificity; D016477:Artifacts; D016207:Cytokines / Q000032:analysis*; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000592:standards; D011487:Protein Conformation; D018121:Receptors, Cytokine / Q000378:metabolism; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D013048:Specimen Handling
D016207:Cytokines; D000939:Epitopes; D018121:Receptors, Cytokine; D011994:Recombinant Proteins