title: Repression of preprotachykinin-A promoter activity is mediated by a proximal promoter element.
abstract: The rat preprotachykinin-A promoter, which is able to direct reporter gene expression in adult dorsal root ganglia neurons grown in culture, has no detectable activity in HeLa and PC12 cells. DNAase 1 footprinting and electrophoretic mobility shift analyses with HeLa nuclear extract indicated the presence of a protein complex binding to a region of the rat preprotachykinn-A gene promoter between the TATA box and the major transcriptional start site. We demonstrate that the sequence of the preprotachykinin-A promoter spanning nucleotides -47 to +92 functions to repress reporter gene expression in HeLa and PC12 cells but not in adult rat dorsal root ganglia grown in culture, and that this repression is correlated with a protein(s) binding to the element between the TATA box and major transcription initiation site. These results indicate that the tissue-specific expression of the preprotachykinin-A gene could require the interaction of both positive and negative regulatory DNA elements.
authors.affiliation: MRC Brain Metabolism Unit, Royal Edinburgh Hospital, U.K.
authors.forename: S C
authors.initials: SC
authors.lastname: Mendelson
authors.forename: C F
authors.initials: CF
authors.lastname: Morrison
authors.forename: J
authors.initials: J
authors.lastname: McAllister
authors.forename: J M
authors.initials: JM
authors.lastname: Paterson
authors.forename: S P
authors.initials: SP
authors.lastname: Dobson
authors.forename: P K
authors.initials: PK
authors.lastname: Mulderry
authors.forename: J P
authors.initials: JP
authors.lastname: Quinn
mesh_terms: D000818:Animals; D001665:Binding Sites; D002478:Cells, Cultured / Q000187:drug effects; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D016716:PC12 Cells / Q000187:drug effects; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D012097:Repressor Proteins; D013126:Spinal Nerve Roots; D015320:Tachykinins / Q000235:genetics* / Q000494:pharmacology; D014158:Transcription, Genetic
chemical_list: D012097:Repressor Proteins; D015320:Tachykinins