A novel 6Fe (2 x [3Fe-4S]) ferredoxin from Mycobacterium smegmatis.
A novel ferredoxin was purified from Mycobacterium smegmatis by a series of hydrophobic chromatographies in the presence of high concentrations of ammonium sulfate and sodium chloride. The ferredoxin exhibited the same peptide map and N-terminal amino acid sequence as the known 7Fe ferredoxin from the same bacterium. On the other hand, this ferredoxin was found to contain approximately 6 Fe/mol ferredoxin and was also shown to contain only [3Fe-4S] clusters by resonance Raman spectroscopy, indicating that it is a novel 6Fe ferredoxin which contains two [3Fe-4S] clusters.
Imai
T
T
Department of Chemistry, Rikkyo (St. Paul's) University, Tokyo, Japan.
Urushiyama
A
A
Saito
H
H
Sakamoto
Y
Y
Ota
K
K
Ohmori
D
D
D004578:Electron Spin Resonance Spectroscopy; D005288:Ferredoxins / Q000737:chemistry / Q000302:isolation & purification*; D009161:Mycobacterium / Q000737:chemistry*; D013059:Spectrum Analysis, Raman
D005288:Ferredoxins