title: Cloning and primary structure of the chiA gene from Aeromonas caviae.
abstract: The chiA gene from Aeromonas caviae encodes an extracellular chitinase, 865 amino acids long, that shows a high degree of similarity to chitinase A of Serratia marcescens. Expression in Escherichia coli yielded an enzymatically active protein from which a leader sequence was removed, presumably during transport of the enzyme across the cell membrane.
authors.affiliation: Otto Warburg Center for Agricultural Biotechnology, Faculty of Agriculture, Hebrew University, Rehovot, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Sitrit
authors.forename: C E
authors.initials: CE
authors.lastname: Vorgias
authors.forename: I
authors.initials: I
authors.lastname: Chet
authors.forename: A B
authors.initials: AB
authors.lastname: Oppenheim
mesh_terms: D000333:Aeromonas / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D001692:Biological Transport; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D002688:Chitinases; C094590:chitinase ChiA