Cloning and primary structure of the chiA gene from Aeromonas caviae.
The chiA gene from Aeromonas caviae encodes an extracellular chitinase, 865 amino acids long, that shows a high degree of similarity to chitinase A of Serratia marcescens. Expression in Escherichia coli yielded an enzymatically active protein from which a leader sequence was removed, presumably during transport of the enzyme across the cell membrane.
Sitrit
Y
Y
Otto Warburg Center for Agricultural Biotechnology, Faculty of Agriculture, Hebrew University, Rehovot, Israel.
Vorgias
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CE
Chet
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I
Oppenheim
A B
AB
D000333:Aeromonas / Q000201:enzymology / Q000235:genetics*; D000595:Amino Acid Sequence; D001692:Biological Transport; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D021382:Protein Sorting Signals; D011994:Recombinant Proteins; D002688:Chitinases; C094590:chitinase ChiA