The essential role of endogenous IFN alpha/beta in the anti-metastatic action of sensitized T lymphocytes in mice injected with Friend erythroleukemia cells.
Adoptive transfer of splenic T lymphocytes from DBA/2 mice immunized against Friend erythroleukemia cells (FLC) inhibited the development of visceral metastases and increased the survival time of DBA/2 mice challenged i.v. with parental FLC 24 hr to 2 months later. Immune spleen cells were ineffective in mice pre-treated with potent neutralizing antibody to mouse IFN alpha/beta (but not to IFN gamma), demonstrating the essential participation of endogenous IFN alpha/beta in the inhibitory action of immune T lymphocytes against FLC metastases. These findings suggest that the reported inability of immune T lymphocytes to exert an anti-FLC effect in immunodeficient DBA/2 mutant beige (bg/bg) mice (unless these mice had also been treated with IFN alpha/beta), may have been due to lower levels of endogenous IFN alpha/beta in DBA/2 bg/bg mice than in normal DBA/2+/bg mice. Experimental results in support of this hypothesis are presented.
Gresser
I
I
Laboratory of Viral Oncology (UPR CNRS 9045), Institut de Recherches sur le Cancer/IFC1, Villejuif, France.
Maury
C
C
Kaido
T
T
Bandu
M T
MT
Tovey
M G
MG
Maunoury
M T
MT
Fantuzzi
L
L
Gessani
S
S
Greco
G
G
Belardelli
F
F
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D005260:Female; D005622:Friend murine leukemia virus; D016219:Immunotherapy, Adoptive; D016898:Interferon-alpha / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D016899:Interferon-beta / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D007371:Interferon-gamma / Q000235:genetics; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology / Q000628:therapy*; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009362:Neoplasm Metastasis; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000906:Antibodies; D016898:Interferon-alpha; D012333:RNA, Messenger; D016899:Interferon-beta; D007371:Interferon-gamma