Alternative to polyacrylamide gels improves the electrophoretic mobility shift assay.
In this paper we outline a simplified protocol for the electrophoretic mobility shift assay utilizing TreviGel 500, a nontoxic alternative to polyacrylamide. The TreviGel 500 matrix combines the strength and resolution of polyacrylamide with the simplicity and flexibility of agarose in the casting of gels. Therefore, this method provides a simple, rapid and nontoxic alternative to current protocols for the investigation of protein: DNA interactions.
Vanek
P G
PG
Department of Biochemistry, Georgetown University Medical Center, Washington, DC 20007, USA.
Fabian
S J
SJ
Fisher
C L
CL
Chirikjian
J G
JG
Collier
G B
GB
D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000032:analysis; D004586:Electrophoresis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight
D004268:DNA-Binding Proteins; D005782:Gels