SacNI, an isoschizomer of BanII isolated from Streptomyces achromogenes recognizes the 5'-GRGCY/C sequence.
SacNI, an isoschizomer of the restriction endonuclease, BanII [Sugisaki et al., Nucleic Acids Res. 10 (1982) 5747-5752], has been isolated from Streptomyces achromogenes N-J-H. SacNI recognizes the palindromic sequence, 5'-GRGCY/C, and cleaves within the recognition sequence, generating a 3' protruding RGCY end (where R = A or G, and Y = C or G).
Rutkowska
S M
SM
Department of Microbiology, University of Gdańsk, Poland.
Skowron
P M
PM
Bielawski
K
K
Podhajska
A J
AJ
D001483:Base Sequence; D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity
D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific; C051481:GRGCYC-specific type II deoxyribonucleases