title: Technical note: tissue residues of mimosine and 2,3-dihydroxypyridine after intravenous infusion in goats.
abstract: Sixteen growing Alpine wethers (average BW 35 +/- 2 kg) were assigned to one of four treatments to evaluate tissue retention of the leucaena toxins mimosine (MIM) and 2,3-dihydroxypyridine (2,3-DHP). Treatments were infused i.v. for 2 d and were 1) saline control, 2) MIM (200 mg.kg BW-.75.d-1), 3) 2,3-DHP (200 mg.kg BW-.75.d-1), or 4) MIM (100 mg.kg BW-.75.d-1) + 2,3-DHP (100 mg.kg BW-.75.d-1). Immediately after the infusion, the goats were slaughtered and tissue concentrations of MIM and 2,3-DHP were determined via HPLC. No detectable levels of either toxin were found in spleen, heart, lung, or muscle; however, appreciable amounts of MIM and 2,3-DHP were found in plasma, kidney, and liver samples. Kidney MIM content was greater (P < .01) than that of liver, although liver tended to retain slightly more 2,3-DHP (P > .05). Infusion of MIM resulted in a plasma MIM content of 39 to 54 mumol/L and reduced (P < .01) plasma PHE and LEU. Infusion of 2,3-DHP resulted in a plasma 2,3-DHP content of 9.4 mumol/L and increased plasma THR, ARG, VAL, PHE, ILE, LEU, and LYS concentrations (P < .10). Humans consuming offals from ruminants consuming large amounts of the leguminous forage leucaena may be exposed to appreciable quantities of MIM and 2,3-DHP.
authors.affiliation: E. (Kika) de la Garza Institute for Goat Research, Langston University, OK 73050.
authors.forename: T
authors.initials: T
authors.lastname: Sahlu
authors.forename: R
authors.initials: R
authors.lastname: Puchala
authors.forename: P J
authors.initials: PJ
authors.lastname: Reis
authors.forename: J J
authors.initials: JJ
authors.lastname: Davis
authors.forename: K
authors.initials: K
authors.lastname: Tesfai
authors.forename: J M
authors.initials: JM
authors.lastname: Fernandez
authors.forename: A A
authors.initials: AA
authors.lastname: Millamena
mesh_terms: D000818:Animals; D001120:Arginine / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues / Q000032:analysis*; D006041:Goats / Q000378:metabolism*; D007262:Infusions, Intravenous; D007668:Kidney / Q000737:chemistry* / Q000378:metabolism; D007930:Leucine / Q000097:blood; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D008898:Mimosine / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood; D011725:Pyridines / Q000008:administration & dosage / Q000032:analysis* / Q000097:blood; D011897:Random Allocation; D014633:Valine / Q000097:blood
chemical_list: D011725:Pyridines; D008898:Mimosine; C035877:2,3-dihydroxypyridine; D001120:Arginine; D007930:Leucine; D014633:Valine