Receptor-binding properties of the peptides corresponding to the ACTH-like sequence of human pro-interleukin-1 alpha.
The octapeptide Gly-Lys-Val-Leu-Lys-Lys-Arg-Arg (termed leukocorticotropin, LCT) corresponding to the ACTH-like sequence 81-88 of human pro-interleukin-1 alpha and its derivative Tyr-Gly-Lys-Val-Leu-Lys-Lys-Arg-Arg were synthesized. The 125I-labeled Tyr-LCT specifically interacts with one type of receptor on the surface of murine splenocytes (Kd = (1.45 +/- 0.04) x 10(-8) M, the number of binding sites is equal to 4500) and with two types of receptors on the surface of murine peritoneal macrophages (Kd1 = (5.9 +/- 1.0) x 10(-9) M and Kd2 = (2.6 +/- 2.2) x 10(-7) M). LCT and Tyr-LCT significantly increase the adenylate cyclase activity of murine peritoneal macrophages. The receptor binding and adenylate cyclase stimulation activity of LCT and Tyr-LCT are inhibited by ACTH (13-24).
Zav'yalov
V P
VP
Institute of Immunology, Moscow Region, Chekhov District, Russia.
Maiorov
V A
VA
Safonova
N G
NG
Navolotskaya
E V
EV
Volodina EYu
Abramov
V M
VM
D000262:Adenylyl Cyclases / Q000187:drug effects; D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive / Q000502:physiology; D006801:Humans; D007375:Interleukin-1 / Q000737:chemistry* / Q000276:immunology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry* / Q000276:immunology; D011498:Protein Precursors / Q000737:chemistry* / Q000276:immunology; D018043:Receptors, Corticotropin / Q000737:chemistry* / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000166:cytology
D007375:Interleukin-1; D010446:Peptide Fragments; D011498:Protein Precursors; D018043:Receptors, Corticotropin; D000262:Adenylyl Cyclases