title: Comparison of the cDNA and amino acid sequences of lipoprotein lipase in eight species.
abstract: By aligning nucleotide and amino acid sequences of lipoprotein lipase in eight species (man, pig, cow, sheep, mouse, rat, guinea-pig and chicken), we found that the main domains (catalytic, N-glycosylation and putative heparin binding sites) are well conserved. The longest identical amino acid chain was encoded by a sequence between the end of exon 2 and the beginning of exon 3, emphasizing the importance of this region which encodes the beta 5-loop of the active site, among other domains. Exon 10 is entirely untranslated in the seven mammals studied here and contains species-characteristic deletions, insertions or elements rich in A or A + T. In chicken, the beginning of exon 10 is translated. These eight previously unreported alignments could be a useful tool for further studies on LPL function.
authors.affiliation: CHU Pitié-Salpêtrière, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Raisonnier
authors.forename: J
authors.initials: J
authors.lastname: Etienne
authors.forename: F
authors.initials: F
authors.lastname: Arnault
authors.forename: D
authors.initials: D
authors.lastname: Brault
authors.forename: L
authors.initials: L
authors.lastname: Noé
authors.forename: J C
authors.initials: JC
authors.lastname: Chuat
authors.forename: F
authors.initials: F
authors.lastname: Galibert
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D053304:Apolipoprotein C-II; D001055:Apolipoproteins B / Q000378:metabolism; D001056:Apolipoproteins C / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D005075:Biological Evolution; D002417:Cattle; D002645:Chickens; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005091:Exons; D006031:Glycosylation; D006168:Guinea Pigs; D006493:Heparin / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D008074:Lipoproteins / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011485:Protein Binding; D051381:Rats; D011973:Receptors, LDL / Q000502:physiology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep; D013552:Swine
chemical_list: D053304:Apolipoprotein C-II; D001055:Apolipoproteins B; D001056:Apolipoproteins C; D018076:DNA, Complementary; D008074:Lipoproteins; D011973:Receptors, LDL; D006493:Heparin; D008071:Lipoprotein Lipase