title: BIAcore as a tool in antibody engineering.
abstract: The BIAcore biosensor provides a simple and rapid approach for analysing recombinant antibodies and phage displayed antibody libraries. In this review we describe the application of the biosensor in the screening of recombinant antibody fragments, kinetic selection of phage displayed antibodies, characterization and epitope mapping of monoclonal antibodies and their fragments.
authors.affiliation: Department of Immunotechnology, Lund University, Sweden.
authors.forename: A C
authors.initials: AC
authors.lastname: Malmborg
authors.forename: C A
authors.initials: CA
authors.lastname: Borrebaeck
mesh_terms: D000486:Allergy and Immunology / Q000295:instrumentation*; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation / Q000276:immunology; D001435:Bacteriophages / Q000276:immunology; D015374:Biosensing Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping / Q000295:instrumentation; D006801:Humans; D011994:Recombinant Proteins / Q000276:immunology
chemical_list: D000906:Antibodies; D011994:Recombinant Proteins