Development of rapid mercury assays. Synthesis of sulfur- and mercury-containing conjugates.
We have devised rapid analyses for mercury exploiting the high affinity of dithiocarbamate chelators for the mercuric ion. Our first assay is based on a sandwich chelate formed by a ligand supported on the well of an ELISA plate, Hg2+ ion of the investigated sample, and another ligand bound to a reporter enzyme. The second assay utilizes competitive binding of the analyte Hg2+ ions versus an organomercury conjugate to a chelating conjugate. Low ppb sensitivity and high selectivity for Hg2+ ions have been achieved in our pilot studies.
Szurdoki
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F
Department of Entomology, University of California, Davis 95616, USA.
Kido
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H
Hammock
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BD
D002614:Chelating Agents / Q000138:chemical synthesis* / Q000737:chemistry; D008024:Ligands; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D013455:Sulfur / Q000737:chemistry*; D013859:Thiocarbamates / Q000138:chemical synthesis* / Q000737:chemistry
D002614:Chelating Agents; D008024:Ligands; D011108:Polymers; D011506:Proteins; D013859:Thiocarbamates; D013455:Sulfur; D008628:Mercury