title: Gsh-2, a murine homeobox gene expressed in the developing brain.
abstract: A novel murine dispersed homeobox gene, designated Gsh-2, is described. Analysis of cDNA sequence, including the full open reading frame, reveals an encoded homeodomain that is surprisingly similar to those of the Antennapedia-type clustered Hox genes. In addition, the encoded protein includes polyhistidine and polyalanine tracts, as observed for several other genes of developmental significance. In situ hybridizations showed Gsh-2 expression in the developing central nervous system, including the ganglionic eminences of the forebrain, the diencephalon, which gives rise to the thalamus and hypothalamus, and in the hindbrain. Furthermore, a random oligonucleotide selection and PCR amplification procedure was used to define a target DNA binding sequence, CNAATTAG, as a first step towards the identification of downstream target genes.
authors.affiliation: Children's Hospital Research Foundation, Department of Pediatrics, University of Cincinnati College of Medicine, Ohio, USA.
authors.forename: H M
authors.initials: HM
authors.lastname: Hsieh-Li
authors.forename: D P
authors.initials: DP
authors.lastname: Witte
authors.forename: J C
authors.initials: JC
authors.lastname: Szucsik
authors.forename: M
authors.initials: M
authors.lastname: Weinstein
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: S S
authors.initials: SS
authors.lastname: Potter
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005801:Genes, Homeobox; D051379:Mice; D008969:Molecular Sequence Data