Interaction of organophosphorus analogues of amino acids with P450.
1. This study deals with the oxidation of organophosphorus amino acid analogues by phenobarbital-induced rabbit liver microsomes. It has been shown that 1-aminoalkylphosphonous and 1-aminoalkylthiophosphonic acids are converted by P450 to 1-aminoalkylphosphonic acids. 2. Phosphonous analogues of amino acids cause type I spectral changes, and thiophosphonic analogues produce reverse type I changes in different spectra. 3. In the presence of NADPH, the 1-aminoalkylphosphonous acids form the corresponding 1-aminoalkylphosphonic acids by the reaction P-H-->P-OH, as monitored using 1H nmr spectroscopy. 4. Aminoalkylthiophosphonic acids have also proven to be the substrates for the NADPH-dependent monoxygenase system. During the course of oxidative desulphuration 1-aminoalkylphosphonic acids were formed by the reaction P = S-->P = O, as monitored by 31P-nmr spectroscopy. 5. Using resonance Raman (RR) spectroscopy, the interaction of 1-aminoisobutyl-phosphonous acid with P450 was investigated, and characteristic changes in spectral frequencies in the region between 1370 and 1700 cm-1 were demonstrated. These latter changes indicate that substrate binding of organophosphorus compounds leads to alterations in haem conformation and to redistribution of the electron density.
Shumyantseva
V V
VV
Institute of Biomedical Chemistry, Moscow, Russia.
Meshkov
S V
SV
Ivanov YuD
Alexandrova
O V
OV
Uvarov VYu
Archakov
A I
AI
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D008958:Models, Molecular; D009249:NADP / Q000494:pharmacology; D063065:Organophosphonates / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology; D017976:Phosphorus Acids / Q000378:metabolism; D011817:Rabbits; D013059:Spectrum Analysis, Raman; D013379:Substrate Specificity
D000596:Amino Acids; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D017976:Phosphorus Acids; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital