Gsh-2, a murine homeobox gene expressed in the developing brain.
A novel murine dispersed homeobox gene, designated Gsh-2, is described. Analysis of cDNA sequence, including the full open reading frame, reveals an encoded homeodomain that is surprisingly similar to those of the Antennapedia-type clustered Hox genes. In addition, the encoded protein includes polyhistidine and polyalanine tracts, as observed for several other genes of developmental significance. In situ hybridizations showed Gsh-2 expression in the developing central nervous system, including the ganglionic eminences of the forebrain, the diencephalon, which gives rise to the thalamus and hypothalamus, and in the hindbrain. Furthermore, a random oligonucleotide selection and PCR amplification procedure was used to define a target DNA binding sequence, CNAATTAG, as a first step towards the identification of downstream target genes.
Hsieh-Li
H M
HM
Children's Hospital Research Foundation, Department of Pediatrics, University of Cincinnati College of Medicine, Ohio, USA.
Witte
D P
DP
Szucsik
J C
JC
Weinstein
M
M
Li
H
H
Potter
S S
SS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005801:Genes, Homeobox; D051379:Mice; D008969:Molecular Sequence Data