Properties of base-pairing in the stem region of hairpin antisense oligonucleotides containing 2'-methoxynucleosides.
We have designed a new type of antisense oligodeoxyribonucleotide. These oligonucleotides are able to form hairpin loop structures at the 3'-ends. The stability to nuclease degradation was observed by incubation of these hairpin oligonucleotides with snake venom phosphodiesterase, DNA polymerase, and fetal bovine serum. Of particular interest is the hairpin antisense oligonucleotide containing 2'-methoxynucleosides with base-pairing in the stem region at the 3'-end, which has increased nuclease resistance.
Hosono
K
K
Department of Industrial Chemistry, Chiba Institute of Technology, Japan.
Kuwasaki
T
T
Tsukahara
S
S
Takai
K
K
Takaku
H
H
D001482:Base Composition; D001483:Base Sequence; D004256:DNA Polymerase I / Q000378:metabolism; D004355:Drug Stability; D015694:Gene Products, rev / Q000235:genetics; D015497:HIV-1 / Q000235:genetics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000378:metabolism; D043264:Phosphodiesterase I; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D016914:Ribonuclease H / Q000378:metabolism; D054321:rev Gene Products, Human Immunodeficiency Virus
D015694:Gene Products, rev; D016376:Oligonucleotides, Antisense; D054321:rev Gene Products, Human Immunodeficiency Virus; D004256:DNA Polymerase I; D016914:Ribonuclease H; D010727:Phosphoric Diester Hydrolases; D043264:Phosphodiesterase I; C470790:phosphodiesterase I, snake venom