title: Increased growth of brown adipose tissue but its reduced thermogenic activity in creatine-depleted rats fed beta-guanidinopropionic acid.
abstract: To study the responses of thermogenic activity in brown adipose tissue (BAT) to creatine depletion, male Wistar rats were fed creatine analogue beta-guanidinopropionic acid (beta-GPA) for about 10 weeks. Compared to control rats, a marked decrease in the levels of high-energy phosphates, such as phosphocreatine and ATP, was noted in BAT of beta-GPA rats. Conversely, upward trends in other chemical components (DNA, glycogen, and total protein) in BAT as well as an increase in BAT mass were observed in beta-GPA rats, suggesting a tendency to hyperplasia of the BAT. The thermogenic activity (which was assessed by guanosine 5'-diphosphate binding to BAT mitochondria) in the mitochondria recovered from BAT of beta-GPA rats, however, was not increased in response to such changes but rather decreased. Moreover, uncoupling protein (UCP) content in the mitochondrial fraction of beta-GPA rats was significantly lower than that in control rats (the relative amounts were 77 +/- 6 and 100 +/- 4%, respectively). Nevertheless, surprisingly, the level of UCP mRNA was remarkably greater in beta-GPA rats than in control rats. These observations indicate that there is a discordance between BAT growth and activity in beta-GPA rats, thereby suggesting that a failure on and after UCP translation may be involved in the impairment of BAT thermogenic activity with creatine depletion. The impairment of BAT thermogenic activity, that is, UCP activity may indicate that uncoupling or heat production was inhibited in order to increase the ATP synthesis in BAT of beta-GPA rats in compensation for a reduction in the levels of high-energy phosphates (including ATP), with resultant hypothermia.
authors.affiliation: Department of Hygiene, National Defense Medical College, Tokorozawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamashita
authors.forename: Y
authors.initials: Y
authors.lastname: Ohira
authors.forename: T
authors.initials: T
authors.lastname: Wakatsuki
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: T
authors.initials: T
authors.lastname: Kizaki
authors.forename: S
authors.initials: S
authors.lastname: Oh-ishi
authors.forename: H
authors.initials: H
authors.lastname: Ohno
mesh_terms: D002001:Adipose Tissue, Brown / Q000254:growth & development* / Q000503:physiopathology; D000818:Animals; D001835:Body Weight; D003401:Creatine / Q000172:deficiency*; D004032:Diet; D006146:Guanidines / Q000008:administration & dosage*; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D011422:Propionates / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D013696:Temperature
chemical_list: D006146:Guanidines; D011422:Propionates; D003401:Creatine; C005775:guanidinopropionic acid