title: The relationship between worm burden and levels of a circulating antigen (CAA) of five species of Schistosoma in mice.
abstract: This study examines the ability of an assay which measures the amount of a schistosome specific antigen (CAA) in the host circulation to reliably reflect relative worm burden. Mice were infected with 5 species of schistosome with a range of infection dose. The levels of serum CAA increased during schistosome maturation. In all species tested CAA levels correlated well with adult worm burden once the parasites achieved sexual maturity and remained relatively stable during the establishment of egg production. The amount of CAA produced varied between species but within each species CAA levels were proportional to worm numbers: no density-dependent effects on CAA levels were observed even when mice carried worm burdens that were very large relative to host size. T-cell deprivation of the host had no effect on the CAA/worm burden relationship in either Schistosoma mansoni or S. haematobium infections and the CAA equilibrium was unaltered in intact mice when reduction of worm fecundity occurred. These data support the use of the CAA as an accurate and robust estimate of relative schistosome burden in man.
authors.affiliation: Wellcome Centre for Parasitic Infections, Imperial College of Science, Technology and Medicine, London, UK.
authors.forename: A
authors.initials: A
authors.lastname: Agnew
authors.forename: A J
authors.initials: AJ
authors.lastname: Fulford
authors.forename: N
authors.initials: N
authors.lastname: De Jonge
authors.forename: F W
authors.initials: FW
authors.lastname: Krijger
authors.forename: M
authors.initials: M
authors.lastname: Rodriguez-Chacon
authors.forename: V
authors.initials: V
authors.lastname: Gutsmann
authors.forename: A M
authors.initials: AM
authors.lastname: Deelder
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D015801:Helminth Proteins / Q000097:blood*; D008212:Lymphocyte Depletion; D008297:Male; D051379:Mice; D009929:Organ Size; D010270:Parasite Egg Count; D012547:Schistosoma / Q000254:growth & development / Q000276:immunology*; D012552:Schistosomiasis / Q000469:parasitology* / Q000473:pathology; D013154:Spleen / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D006023:Glycoproteins; D015801:Helminth Proteins; C090907:circulating anodic antigen, Schistosoma mansoni