title: Altered sequence recognition specificity of a C5-DNA methyltransferase carrying a chimeric 'target recognizing domain'.
abstract: A MTase with a chimeric TRD with the N-terminal half derived from a TRD recognizing GCNGC, the C-terminal half from one with CCWGG recognition, was constructed. Its target specificity is reported.
authors.affiliation: Max-Planck-Institut für molekulare Genetik, Berlin, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Lange
authors.forename: C
authors.initials: C
authors.lastname: Wild
authors.forename: T A
authors.initials: TA
authors.lastname: Trautner
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D004248:DNA (Cytosine-5-)-Methyltransferases