Immunogenetic analysis of HLA-DR10 homozygous individuals.
Molecular, cellular and serological analysis of Major Histocompatibility Complex alleles was performed on three individuals who appeared to be HLA-DR10 homozygous by DNA restriction fragment analysis. Each donor was of different ethnic origin: Caucasoid, Asian Indian and African Negroid. The results of our studies show that the Caucasoid and Asian donors are indeed homozygous for the HLA-DR10 allele, while the African donor also possesses the DRB1*0103 allele. Homozygosity for the HLA-A1-B37-Cw6-DR10-DQ5 haplotype in the Caucasoid donor was confirmed by familial segregation analysis. The B-lymphoblastoid cell line produced from this donor should prove useful in studies of HLA immunogenetics.
Young
N T
NT
Nuffield Department of Surgery, University of Oxford, UK.
Thompson
J
J
Gibson
K
K
Dewar
K
K
Darke
C
C
D000483:Alleles; D001402:B-Lymphocytes / Q000276:immunology; D044383:Black People / Q000235:genetics; D002461:Cell Line, Transformed; D005787:Gene Frequency; D005802:Genes, MHC Class II; D006680:HLA Antigens / Q000235:genetics; D006684:HLA-DR Antigens / Q000235:genetics*; D006239:Haplotypes / Q000235:genetics; D006720:Homozygote; D006801:Humans; D007194:India / Q000208:ethnology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012675:Senegal / Q000208:ethnology; D006113:United Kingdom; D044465:White People / Q000235:genetics
D006680:HLA Antigens; D006684:HLA-DR Antigens