Glucocorticoid-suppressible hyperaldosteronism and adrenal tumors occurring in a single French pedigree.
Glucocorticoid-suppressible hyperaldosteronism is a dominantly inherited form of hypertension believed to be caused by the presence of a hybrid CYP11B1/CYP11B2 gene which has arisen from an unequal crossing over between the two CYP11B genes in a previous meiosis. We have studied a French pedigree with seven affected individuals in which two affected individuals also have adrenal tumors and two others have micronodular adrenal hyperplasia. One of the adrenal tumors and the surrounding adrenal tissue has been removed, giving a rare opportunity to study the regulation and action of the hybrid gene causing the disease. The hybrid CYP11B gene was demonstrated to be expressed at higher levels than either CYP11B1 or CYP11B2 in the cortex of the adrenal by RT-PCR and Northern blot analysis. In situ hybridization showed that both CYP11B1 and the hybrid gene were expressed in all three zones of the cortex. In cell culture experiments hybrid gene expression was stimulated by ACTH leading to increased production of aldosterone and the hybrid steroids characteristic of glucocorticoid-suppressible hyperaldosteronism. The genetic basis of the adrenal pathologies in this family is not known but may be related to the duplication causing the hyperaldosteronism.
Pascoe
L
L
INSERM U36, Collège de France, Paris, France.
Jeunemaitre
X
X
Lebrethon
M C
MC
Curnow
K M
KM
Gomez-Sanchez
C E
CE
Gasc
J M
JM
Saez
J M
JM
Corvol
P
P
D000310:Adrenal Gland Neoplasms / Q000235:genetics* / Q000378:metabolism; D000311:Adrenal Glands / Q000201:enzymology* / Q000473:pathology; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D001483:Base Sequence; D002478:Cells, Cultured; D019405:Cytochrome P-450 CYP11B2; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D005260:Female; D005602:France; D015971:Gene Expression Regulation, Enzymologic; D005938:Glucocorticoids / Q000378:metabolism*; D006801:Humans; D006929:Hyperaldosteronism / Q000235:genetics* / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D010375:Pedigree; D016133:Polymerase Chain Reaction; D013252:Steroid 11-beta-Hydroxylase / Q000235:genetics*
D005938:Glucocorticoids; D000804:Angiotensin II; D000324:Adrenocorticotropic Hormone; D003577:Cytochrome P-450 Enzyme System; D019405:Cytochrome P-450 CYP11B2; D013252:Steroid 11-beta-Hydroxylase