title: Molecular cloning of the complementary DNA for the mouse pyruvate kinase M-2 gene whose expression is dependent upon cell differentiation.
abstract: A cDNA encoding M2-type pyruvate kinase from mouse was cloned and its nucleotide sequence was determined. The cDNA encoded a protein containing 531 amino acids, the nucleotide and amino acid sequences of which were 93.2% and 98.1%, respectively, homologous to those of rat M2 pyruvate kinase. Expression of the pyruvate kinase in mouse embryonal carcinoma P19 cells altered according to differentiation stages; high at undifferentiated and low at differentiated stages.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Izumi
authors.forename: A
authors.initials: A
authors.lastname: Manabe
authors.forename: A
authors.initials: A
authors.lastname: Tomoyasu
authors.forename: F
authors.initials: F
authors.lastname: Kihara-Negishi
authors.forename: H
authors.initials: H
authors.lastname: Ariga
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism*; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D011770:Pyruvate Kinase / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D011770:Pyruvate Kinase