title: Identification of two opaque2 modifier loci in quality protein maize.
abstract: Genetic modifiers of opaque2 convert the soft, starchy endosperm of opaque2 maize mutants to a hard, vitreous phenotype, while maintaining the enhanced lysine content of the grain. Genetic analysis of F2 segregating seeds from crosses of opaque2 by modified opaque2 genotypes indicated that the modifiers are complex traits that act codominantly. We developed two different segregating F2 populations and mapped the modifier loci by restriction fragment length polymorphism (RFLP) analysis. A relationship was found between formation of vitreous endosperm and the locus encoding the gamma-zein storage protein, which maps near the centromere of chromosome 7. Endosperm modification was consistently associated with the presence of two rather than one gamma-zein gene at this locus. A second modifier locus was mapped near the telomere of chromosome 7L.
authors.affiliation: University of Arizona, Department of Plant Sciences, Tucson, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Lopes
authors.forename: K
authors.initials: K
authors.lastname: Takasaki
authors.forename: D E
authors.initials: DE
authors.lastname: Bostwick
authors.forename: T
authors.initials: T
authors.lastname: Helentjaris
authors.forename: B A
authors.initials: BA
authors.lastname: Larkins
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D010940:Plant Proteins; D014157:Transcription Factors; C067402:opaque-2 protein, Zea mays