title: Luteolytic potency of a prostaglandin analogue at different stages of the oestrous cycle in red deer (Cervus elaphus) hinds.
abstract: The luteolytic effect of the prostaglandin F2 alpha analogue, cloprostenol, was investigated in red deer by monitoring concentrations of plasma progesterone, the induction of oestrus and ovulation, and fertility. Oestrus was synchronized in 48 adult hinds by intravaginal delivery of exogenous progesterone for 12 days and i.m. injection of 250 iu pregnant mares' serum gonadotrophin at progesterone withdrawal. A single i.m. dose of 500 micrograms cloprostenol was administered at day 4, 6, 8, 10, 12, 14 or 16 of the subsequent oestrous cycle (n = 6 hinds per treatment; day 0 = oestrus). Six other hinds were monitored by intensive collection of blood samples between day 16 and day 19 to define changes in plasma progesterone concentrations during spontaneous luteolysis. Samples of jugular blood, collected every second day throughout the study and every 6 h for 78 h from the time of administration of cloprostenol, were analysed for plasma concentrations of progesterone and LH. Oestrus was detected by continuous observation during the period of intensive collection of blood samples and all hinds were subjected to transrectal ultrasonography to assess pregnancy status. On the basis of changes in plasma progesterone concentrations, cloprostenol induced complete luteolysis in all hinds treated on days 8-16 and in five of six hinds treated on day 6. Oestrus, ovulation and conception occurred in 25 (69%), 28 (78%) and 25 (69%), respectively, of hinds treated on days 6-16 inclusive (n = 36). Luteolysis was incomplete in all hinds treated on day 4, and none of the animals exhibited oestrus or ovulated; luteolysis was incomplete for one hind treated on day 6.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: AgResearch, Invermay Agricultural Centre, Mosgiel, New Zealand.
authors.forename: G W
authors.initials: GW
authors.lastname: Asher
authors.forename: M W
authors.initials: MW
authors.lastname: Fisher
authors.forename: D K
authors.initials: DK
authors.lastname: Berg
authors.forename: F A
authors.initials: FA
authors.lastname: Veldhuizen
authors.forename: C J
authors.initials: CJ
authors.lastname: Morrow
mesh_terms: D000818:Animals; D003008:Cloprostenol / Q000494:pharmacology*; D003670:Deer / Q000378:metabolism*; D004971:Estrus / Q000502:physiology*; D005260:Female; D005298:Fertility / Q000187:drug effects; D006064:Gonadotropins, Equine / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000097:blood; D003341:Luteolysis; D010060:Ovulation / Q000187:drug effects; D011374:Progesterone / Q000097:blood / Q000494:pharmacology; D013997:Time Factors
chemical_list: D006064:Gonadotropins, Equine; D003008:Cloprostenol; D011374:Progesterone; D007986:Luteinizing Hormone