Climbing fibre destruction triggers mossy fibre sprouting in adult rat cerebellum.
Molecular layers of the adult rat cerebellum, the inferior olivary neurones and climbing fibres which had been destroyed by 3-acetylpyridine (3-AP) and inferior cerebellar pedunculotomy, were examined using an antibody against corticotropin releasing factor (CRF) to visualize mossy fibres. Sprouting mossy fibres appeared in the molecular layer 2 days after 3-AP treatment. Morphologically, they showed no bundle formation, rosette-like swellings along their course, made ectopic synapses with interneurones, and had no association with arrested granule cells. The number of sprouting fibres gradually increased with time after 3-AP treatment. These findings indicate that mossy fibre sprouting is continuously induced in the climbing fibre-deficient cerebellum.
Murase
S
S
Department of Anatomy, School of Medicine, Keio University, Tokyo, Japan.
D000344:Afferent Pathways / Q000187:drug effects; D000818:Animals; D002531:Cerebellum / Q000187:drug effects* / Q000648:ultrastructure; D007150:Immunohistochemistry; D007274:Injections, Intraperitoneal; D008297:Male; D008636:Mesencephalon / Q000187:drug effects; D009847:Olivary Nucleus / Q000187:drug effects* / Q000648:ultrastructure; D017729:Presynaptic Terminals / Q000187:drug effects*; D011725:Pyridines; D051381:Rats; D011916:Rats, Inbred F344
D011725:Pyridines; C010835:3-acetylpyridine