title: Short report: isolation and partial characterization of a Puumala virus from a human case of nephropathia epidemica in France.
abstract: Puumala (PUU) virus (Bunyaviridae: Hantavirus), the etiologic agent of nephropathia epidemica (NE), the mid form of hemorrhagic fever with renal syndrome, is enzootic in Europe and has been known to occur in France since 1983. We report the first isolation of PUU virus in France and western Europe from a case of NE acquired in France. The virus was isolated from a serum collected in the acute phase of the clinical course by successive blind passages in Vero E6 cells. Serologic typing using monoclonal antibodies confirmed the identity of the virus as PUU. The sequence of an 832-nucleotide fragment of the virus medium RNA segment obtained by the polymerase chain reaction (PCR) also classified it as a PUU virus. The sequence of this isolate from a human case in France is closely related to the sequence of a PUU virus obtained by the PCR from a German patient.
authors.affiliation: Centre National de Reference pour les Fievres Hemorragiques Virales, Institut Pasteur, Paris.
authors.forename: P E
authors.initials: PE
authors.lastname: Rollin
authors.forename: M D
authors.initials: MD
authors.lastname: Bowen
authors.forename: H
authors.initials: H
authors.lastname: Kariwa
authors.forename: J F
authors.initials: JF
authors.lastname: Saluzzo
authors.forename: S
authors.initials: S
authors.lastname: Guerard
authors.forename: A
authors.initials: A
authors.lastname: Flechaire
authors.forename: D
authors.initials: D
authors.lastname: Coudrier
authors.forename: P
authors.initials: P
authors.lastname: Sureau
authors.forename: C J
authors.initials: CJ
authors.lastname: Peters
authors.forename: S T
authors.initials: ST
authors.lastname: Nichol
mesh_terms: D000328:Adult; D000818:Animals; D002522:Chlorocebus aethiops; D005602:France; D005838:Genotype; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D018778:Hantavirus Infections / Q000821:virology*; D006801:Humans; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D012692:Serial Passage; D012703:Serotyping; D014709:Vero Cells
chemical_list: D012367:RNA, Viral