title: Reduced virulence of Candida albicans PHR1 mutants.
abstract: Candida albicans mutants lacking PHR1 exhibit a pH-dependent morphogenic defect which is expressed at pH 7.5, a pH comparable to that of mammalian blood (S. M. Saporito-Irwin, C. E. Birse, P. S. Sypherd, and W. A. Fonzi, Mol. Cell. Biol. 15:601-613, 1995). The in vivo relevance of this expression pattern was tested in a mouse model of systemic candidiasis. A phr1/phr1 mutant was found to be less virulent than an isogenic Phr1+ strain and exhibited altered morphological development in vivo. These results indicate that PHR1 contributes to the virulence of C. albicans.
authors.affiliation: Division of Infectious Diseases, Harbor-UCLA Research and Education Institute, St. John's Cardiovascular Research Center, Torrance 90509, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Ghannoum
authors.forename: B
authors.initials: B
authors.lastname: Spellberg
authors.forename: S M
authors.initials: SM
authors.lastname: Saporito-Irwin
authors.forename: W A
authors.initials: WA
authors.lastname: Fonzi
mesh_terms: D000818:Animals; D001051:Apoenzymes; D002176:Candida albicans / Q000235:genetics / Q000472:pathogenicity*; D002177:Candidiasis / Q000382:microbiology*; D004255:Deoxyribodipyrimidine Photo-Lyase; D005656:Fungal Proteins / Q000502:physiology*; D005800:Genes, Fungal; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000382:microbiology; D008562:Membrane Glycoproteins; D051379:Mice; D008807:Mice, Inbred BALB C; D009024:Morphogenesis
chemical_list: D001051:Apoenzymes; D005656:Fungal Proteins; D008562:Membrane Glycoproteins; C091515:PHR1 protein, Candida albicans; D004255:Deoxyribodipyrimidine Photo-Lyase