Photostructurized electrochemical biosensors for bioreactor control and measurement in body fluids.
This article details a new type of biosensor for the simultaneous analysis of glucose, glutamate and glutamine in complex biological fluids like fermentation broths and blood. Simultaneous analysis was made possible by the application of different enzyme layers onto different electrodes of one photostructurized sensor. Photostructuring was done by means of a new developed photopolymer. Preparation of the photopolymer and the enzyme layers as well as the characterization of the sensors thus constructed with respect to linearity, response time and sensitivity are described.
Lobmaier
C
C
Institut für Biochemie und Molekulare Zellbiologie, Universität Wien, Luswig Boltzmann Forschungsstelle für Biochemie, Austria.
Schalkhammer
T
T
Hawa
G
G
Ecker
B
B
Pittner
F
F
D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D001826:Body Fluids / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D005285:Fermentation; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D018698:Glutamic Acid / Q000032:analysis* / Q000097:blood; D005972:Glutaminase; D005973:Glutamine / Q000032:analysis* / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D008839:Microelectrodes; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001786:Blood Glucose; D007202:Indicators and Reagents; D005973:Glutamine; D018698:Glutamic Acid; D005949:Glucose Oxidase; D005972:Glutaminase; D005947:Glucose