CheA, CheW, and CheY are required for chemotaxis to oxygen and sugars of the phosphotransferase system in Escherichia coli.
We carried out studies with Escherichia coli to determine the site at which the methylation-independent pathways for taxis to oxygen and to sugars of the phosphoenolpyruvate:sugar phosphotransferase transport system converge with the methylation-dependent chemotaxis pathways. Using genetic reconstitution of the pathways in a null strain, we determined that all pathways examined required the products of the genes cheA, cheW, and cheY. Thus, we conclude that both the methylation-independent and methylation-dependent pathways converge at CheA, the histidine kinase product of cheA.
Rowsell
E H
EH
Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, California 92350, USA.
Smith
J M
JM
Wolfe
A
A
Taylor
B L
BL
D001426:Bacterial Proteins / Q000235:genetics*; D002633:Chemotaxis / Q000235:genetics*; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology*; D029968:Escherichia coli Proteins; D005632:Fructose / Q000494:pharmacology; D000071677:Histidine Kinase; D008358:Mannose / Q000494:pharmacology; D008565:Membrane Proteins / Q000235:genetics*; D000072236:Methyl-Accepting Chemotaxis Proteins; D010100:Oxygen / Q000494:pharmacology; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics*
D001426:Bacterial Proteins; C489103:CheW protein, E coli; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins; C000607606:cheY protein, E coli; C051236:CheW protein, Bacteria; D005632:Fructose; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D000071677:Histidine Kinase; C000607608:cheA protein, E coli; D008358:Mannose; D010100:Oxygen