Inhibition of intestinal secretion by rice.
Rates of stool output are reduced when cooked rice is incorporated into oral rehydration solutions. We found that a fraction extracted from rice inhibited the response of intestinal epithelial crypt cells to adenosine 3'5'-cyclic monophosphate, a major intracellular mediator of secretion. This response to rice was seen as an inhibition of cell shrinkage and of chloride efflux in fresh suspended guinea pig crypt cells. The active fraction was of low molecular weight (< 1.5 kDa), hydrophobic, and not a peptide or glycoprotein. It may be chloride-channel blocker.
Macleod
R J
RJ
McGill University-Montreal Children's Hospital Research Institute.
Bennett
H P
HP
Hamilton
J R
JR
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000187:drug effects; D002712:Chlorides / Q000378:metabolism; D000242:Cyclic AMP / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D007421:Intestine, Small / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D012275:Oryza / Q000737:chemistry; D010936:Plant Extracts / Q000008:administration & dosage / Q000032:analysis / Q000494:pharmacology; D015532:Rehydration Solutions / Q000008:administration & dosage / Q000494:pharmacology
D018118:Chloride Channels; D002712:Chlorides; D010936:Plant Extracts; D015532:Rehydration Solutions; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP