title: Assessment of minimal residual disease in acute promyelocytic leukaemia with t(15;17) by chromosome painting.
abstract: To detect the minimal residual disease (MRD) in acute promyelocytic leukaemia patients treated with all-trans retinoic acid, we compared the sensitivity of metaphase fluorescence in situ hybridization (FISH) with conventional analysis of G-banded metaphases. 5 out of 6 patients studied at diagnosis showed the t(15;17) translocation. 4 out of 5 patients carrying t(15;17) achieved complete remission and conventional cytogenetic conversion. In 3 cases the whole chromosome painting (WCP) probe 17 discovered one normal chromosome 17 and two fragments indicative of t(15;17) persistence. The FISH-WCP technique seems to be highly sensitive and recommendable in monitoring leukaemias with specific chromosome rearrangements.
authors.affiliation: Department of Medical Sciences, University of Modena, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Temperani
authors.forename: P
authors.initials: P
authors.lastname: Vaccari
authors.forename: F
authors.initials: F
authors.lastname: Giacobbi
authors.forename: G
authors.initials: G
authors.lastname: Gandini
authors.forename: M G
authors.initials: MG
authors.lastname: Ferrari
authors.forename: G
authors.initials: G
authors.lastname: Emilia
mesh_terms: D000328:Adult; D000368:Aged; D002871:Chromosome Banding; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D015473:Leukemia, Promyelocytic, Acute / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics; D008297:Male; D008875:Middle Aged; D018365:Neoplasm, Residual; D014178:Translocation, Genetic; D014212:Tretinoin / Q000627:therapeutic use
chemical_list: D014212:Tretinoin