Abnormalities of the RB1 and DCC tumor suppressor genes: uncommon in human pancreatic adenocarcinoma.
Evidence of RB1 allele loss was found in only 6% of pancreatic cancers, and we found no significant sequence abnormalities nor loss of RB protein expression in a panel of tumors and cell lines. Using reverse transcription-polymerase chain reaction and Southern blot analysis, we found no evidence for loss of DCC expression in pancreatic cancer cell lines, and allele loss only rarely in tumor biopsies. These findings suggest that abnormalities of RB1 and DCC are unlikely to play a major role in pancreatic carcinogenesis.
Barton
C M
CM
Imperial Cancer Research Fund Molecular Pathology Laboratory, Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.
McKie
A B
AB
Hogg
A
A
Bia
B
B
Elia
G
G
Phillips
S M
SM
Ding
S F
SF
Lemoine
N R
NR
D000230:Adenocarcinoma / Q000235:genetics*; D000483:Alleles; D001483:Base Sequence; D017353:Gene Deletion; D015870:Gene Expression; D016163:Genes, DCC / Q000235:genetics*; D016161:Genes, Retinoblastoma / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009154:Mutation; D010190:Pancreatic Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D011233:Precipitin Tests; D017421:Sequence Analysis; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured