Abnormal expression of PDC-E2 on the apical surface of biliary epithelial cells in patients with antimitochondrial antibody-negative primary biliary cirrhosis.
The presence of antimitochondrial antibodies (AMA) is a major criterion for the diagnosis of primary biliary cirrhosis (PBC). Although it is not clear that AMA are involved in the pathogenesis of the disease, the study of these autoantibodies has enabled much information to be accumulated about the specificity of this response. The autoantigens have been identified as components of a functionally related enzyme family, the 2-oxo-acid-dehydrogenase complex. Within this complex, pyruvate dehydrogenase E2 subunit (PDC-E2) has been determined to be the immunodominant autoantigen. Using a panel of mouse monoclonal antibodies and human combinatorial autoantibodies, it has been demonstrated that patients with PBC, but not controls, have an abnormal expression of either PDC-E2 or a cross-reacting molecule in the apical region of biliary epithelium. Others have shown a similar reaction using rabbit sera directed to PDC-E2. Our previous studies have concentrated on AMA-positive patients. In this study, the presence of PDC-E2, class II, immunoglobulin (Ig) A, and B7/BB1 in the bile duct epithelial cells of AMA-positive as well as AMA-negative patients is addressed. Most patients with AMA-negative PBC (seven of nine) react in a fashion similar to AMA-positive patients with intense staining of the apical region of the bile duct epithelial cells of "PDC-E2," increased IgA expression, and little major histocompatibility complex (MHC) class II staining in the early-stage patients. Interestingly, the two AMA-negative patients that did not express PDC-E2 on the apical side of their biliary epithelium had anticentromere antibodies and Sjögren's syndrome.(ABSTRACT TRUNCATED AT 250 WORDS)
Tsuneyama
K
K
Division of Rheumatology, Allergy and Clinical Immunology University of California Davis 95616, USA.
Van De Water
J
J
Van Thiel
D
D
Coppel
R
R
Ruebner
B
B
Nakanuma
Y
Y
Dickson
E R
ER
Gershwin
M E
ME
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001323:Autoantibodies / Q000032:analysis; D001324:Autoantigens / Q000032:analysis; D001653:Bile Ducts, Intrahepatic / Q000201:enzymology / Q000276:immunology*; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; D004848:Epithelium / Q000201:enzymology / Q000276:immunology; D005260:Female; D000949:Histocompatibility Antigens Class II / Q000032:analysis; D006801:Humans; D008105:Liver Cirrhosis, Biliary / Q000201:enzymology / Q000276:immunology*; D008297:Male; D051379:Mice; D018613:Microscopy, Confocal; D008875:Middle Aged; D008928:Mitochondria / Q000276:immunology; D011768:Pyruvate Dehydrogenase Complex / Q000032:analysis*; D011817:Rabbits; D012212:Rheology
D001323:Autoantibodies; D001324:Autoantigens; D000949:Histocompatibility Antigens Class II; D011768:Pyruvate Dehydrogenase Complex; D051047:Dihydrolipoyllysine-Residue Acetyltransferase