Characterization of pPvu1, the autonomous plasmid from Proteus vulgaris that carries the genes of the PvuII restriction-modification system.
Plasmid pPvu1 from Proteus vulgaris carries the genes of the PvuII restriction-modification system [Blumenthal et al., J. Bacteriol. 164 (1985) 501-509]. This report focuses on physical and functional features of the 4.84-kb plasmid, which shows a composite genetic architecture. Plasmid pPvu1 has a replication origin and an incompatibility locus that each function in Escherichia coli, and an apparent cer recombination site. The replication origin includes a possible RNA I gene, and the incompatibility locus closely resembles a rom gene. These loci show substantial sequence similarity to corresponding loci from the E. coli plasmids P15A, ColEI and pSC101, and closely flank the PvuII genes. The close association between a recombinational locus and the PvuII genes has implications for their mobility.
Calvin Koons
M D
MD
Department of Microbiology, Medical College of Ohio, Toledo 43699-0008, USA.
Blumenthal
R M
RM
D000595:Amino Acid Sequence; D001483:Base Sequence; D015257:DNA-Cytosine Methylases / Q000096:biosynthesis / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010957:Plasmids; D011514:Proteus vulgaris / Q000201:enzymology* / Q000235:genetics*; D018741:Replication Origin; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
C047084:DNA modification methylase PvuII; D015257:DNA-Cytosine Methylases; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific