Degradation of trans-ferulic and p-coumaric acid by Acinetobacter calcoaceticus DSM 586.
Cell suspensions of Acinetobacter calcoaceticus strain DSM 586 and DSM 590 were able to grow on benzoic, p-hydroxybenzoic and vanillic acid as sole carbon source. Testing the utilization of trans-ferulic and p-coumaric acid, we found that the sole A. calcoaceticus DSM 586 efficiently degraded the lignocellulose related monomers. Cells induced with trans-ferulic acid were able to oxidize trans-ferulic, p-coumaric, vanillic, p-hydroxybenzoic and protocatechuic acid at rates higher than the uninduced culture. The same activity was found in the p-coumaric acid induced culture. Two aromatic compounds, vanillic and p-hydroxybenzoic acid, were isolated from culture filtrates of trans-ferulic and p-coumaric acid grown cells, respectively, and further characterized by high performance liquid chromatography. 1H- and 13C-nuclear magnetic resonance and ultraviolet spectrophotometry. Cell extracts of trans-ferulic or p-coumaric acid induced cultures were shown to rapidly convert protocatechuic acid to beta-carboxymuconic acid. Moreover, A. calcoaceticus DSM 586 produced high levels of protocatechuic 3,4-dioxygenase compared to cathecol 1,2-dioxygenase and gentisate 1,2-dioxygenase in the degradation of trans-ferulic or p-coumaric acid. Based upon these results, a reaction sequence for the complete degradation of trans-ferulic and p-coumaric acid in A. calcoaceticus DSM 586 is proposed.
Delneri
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Microbiology Department, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
Degrassi
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G
Rizzo
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R
Bruschi
C V
CV
D016954:Acinetobacter calcoaceticus / Q000254:growth & development / Q000378:metabolism*; D050548:Catechol 1,2-Dioxygenase; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000378:metabolism*; D049308:Dioxygenases; D062385:Hydroxybenzoates / Q000378:metabolism; D008031:Lignin / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D010105:Oxygenases / Q000378:metabolism; D010226:Parabens / Q000302:isolation & purification / Q000378:metabolism; D011422:Propionates; D011520:Protocatechuate-3,4-Dioxygenase / Q000378:metabolism; D013053:Spectrophotometry; D014641:Vanillic Acid / Q000302:isolation & purification / Q000378:metabolism
D003373:Coumaric Acids; D062385:Hydroxybenzoates; D010226:Parabens; D011422:Propionates; C009091:protocatechuic acid; D008031:Lignin; C004999:ferulic acid; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; D011520:Protocatechuate-3,4-Dioxygenase; D014641:Vanillic Acid; C495469:p-coumaric acid; C038193:4-hydroxybenzoic acid