Effects of excess doses of alpha-tocopherol on the lipids and function of rainbow trout liver.
Rainbow trout (Oncorhynchus mykiss) were kept on diets containing three different alpha-tocopherol levels (0, 10, and 1,000 mg/100 g diet) to investigate the effects of excess doses of alpha-tocopherol on the liver lipids and liver functions. Both the content of the lipids except alpha-tocopherol in the liver and the triglyceride ratio in total lipids were significantly increased by the diets containing 1,000 mg of alpha-tocopherol per 100 g of diet. Many lipid droplets, 5-20 microns in the diameter, were observed in the liver. The TBARS per milligrams of liver lipids was decreased by 1,000-mg doses of alpha-tocopherol. GOT and GPT activity in serum were in the normal range regardless of the dose of alpha-tocopherol. From these data, it is considered that excess alpha-tocopherol promotes lipid accumulation enough to form droplets in the liver tissue, although liver function is not impaired.
Tokuda
M
M
Laboratory of Marine Biochemistry, Faculty of Agriculture, Tohoku University, Miyagi, Japan.
Takeuchi
M
M
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001835:Body Weight / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis / Q000502:physiology; D008099:Liver / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology; D017686:Oncorhynchus mykiss / Q000378:metabolism / Q000502:physiology*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
D008055:Lipids; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase