Glycosphingolipids in human pancreatic juice. Unique fatty acid compositions of glucosylceramides and lactosylceramides.
Glycosphingolipids in human pancreatic juice were isolated and purified by DEAE and silica gel column chromatographs, and further by HPLC on silica gel and reversed-phase columns. The structures of the glycosphingolipids were determined to be glycosylceramides and lactosylceramides by means of fast atom bombardment mass spectrometry, 1H-NMR spectroscopy, and component analysis involving GLC-mass spectrometry. The ceramide portions of the glucosylceramides consist of palmitic, tetracosanoic, and alpha-hydroxy tetracosanoic fatty acids, and d18:1 and t18:0 sphingosines. The ceramide portions of the lactosylceramides consist of palmitic, teracosanoic, tetracosenoic, and tetracosadienoic fatty acids, and d18:1 sphingosine. The fatty acid compositions are different from the free fatty acid compositions of serum and pancreatic juice. The predominance of saturated, unsaturated, and hydroxy tetracosanoic fatty acid is quite unique, and the possibility that these glycosphingolipids may play a specific role in pancreatic juice deserves consideration. These glycosphingolipids were documented to be major components of the pancreatic juice from 15 patients with a variety of pancreatic diseases.
Hamanaka
Y
Y
Department of Surgery II, Yamaguchi University School of Medicine, Japan.
Hamanaka
S
S
Kaneko
T
T
Suzuki
T
T
Suzuki
M
M
Suzuki
A
A
D000328:Adult; D000368:Aged; D005260:Female; D005963:Glucosylceramides / Q000032:analysis*; D006801:Humans; D007790:Lactosylceramides / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D010189:Pancreatic Juice / Q000737:chemistry*
D005963:Glucosylceramides; D007790:Lactosylceramides