The IL-2 receptor gamma c chain does not function as a subunit shared by the IL-4 and IL-13 receptors. Implication for the structure of the IL-4 receptor.
The IL-2 receptor (IL-2R) gamma c subunit is also a component of the receptors for IL-4, IL-7, IL-9, and IL-15. The IL-4R and IL-13R appear to share a common subunit, and gamma c was proposed to be this shared subunit. In this study, we have assessed the relative contribution of gamma c to the mouse IL-4R and IL-13R. The MC/9 mast cell line constitutively expresses gamma c and proliferates to IL-4 and IL-13, but only the response to IL-4 was blocked by anti-gamma c mAbs. After transfection of the IL-4- and IL-13-responsive gamma c-negative B9 plasmacytoma with full length (m gamma) or cytoplasmic-tailless gamma c cDNA (m gamma t), only the proliferative response to IL-4 was affected by the surface expression of these gamma c molecules. The inability of m gamma or m gamma t expression to affect IL-13-induced proliferation by B9 indicates that gamma c does not obviously contribute to the IL-13R and does not function as the shared subunit of the IL-4R and IL-13R. This study suggests that there are two distinct IL-4R, one of which is independent of gamma c.
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Department of Microbiology and Immunology, University of Miami School of Medicine, FL 33136, USA.
Malek
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D015152:Blotting, Northern; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D007127:Immunoglobulin Constant Regions / Q000737:chemistry / Q000502:physiology; D007142:Immunoglobulin gamma-Chains / Q000737:chemistry / Q000502:physiology; D018793:Interleukin-13 / Q000494:pharmacology; D053661:Interleukin-13 Receptor alpha1 Subunit; D015847:Interleukin-4 / Q000494:pharmacology; D008407:Mast Cells / Q000166:cytology; D051379:Mice; D015394:Molecular Structure; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D018123:Receptors, Interleukin / Q000737:chemistry* / Q000502:physiology; D053660:Receptors, Interleukin-13; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis / Q000737:chemistry* / Q000502:physiology*; D019948:Receptors, Interleukin-4; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; C508183:Il13ra1 protein, mouse; D007127:Immunoglobulin Constant Regions; D007142:Immunoglobulin gamma-Chains; D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053660:Receptors, Interleukin-13; D015375:Receptors, Interleukin-2; D019948:Receptors, Interleukin-4; D015847:Interleukin-4