title: Characterization of toxin-producing cyanobacteria by using an oligonucleotide probe containing a tandemly repeated heptamer.
abstract: Cyanobacteria produce toxins that kill animals. The two main classes of cyanobacterial toxins are cyclic peptides that cause liver damage and alkaloids that block nerve transmission. Many toxin-producing strains from Finnish lakes were brought into axenic culture, and their toxins were characterized. Restriction fragment length polymorphism analysis, probing with a short tandemly repeated DNA sequence found at many locations in the chromosome of Anabaena sp. strain PCC 7120, distinguishes hepatotoxic Anabaena isolates from neurotoxin-producing strains and from Nostoc spp.
authors.affiliation: Department of Applied Chemistry and Microbiology, University of Helsinki, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Rouhiainen
authors.forename: K
authors.initials: K
authors.lastname: Sivonen
authors.forename: W J
authors.initials: WJ
authors.lastname: Buikema
authors.forename: R
authors.initials: R
authors.lastname: Haselkorn
mesh_terms: D001427:Bacterial Toxins / Q000494:pharmacology; D001483:Base Sequence; D000458:Cyanobacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D000087522:Cyanobacteria Toxins; D004791:Enzyme Inhibitors; D005618:Fresh Water; D008387:Marine Toxins; D052998:Microcystins; D008969:Molecular Sequence Data; D009498:Neurotoxins; D015345:Oligonucleotide Probes; D010456:Peptides, Cyclic; D012091:Repetitive Sequences, Nucleic Acid; D014871:Water Microbiology
chemical_list: D001427:Bacterial Toxins; D000087522:Cyanobacteria Toxins; D004791:Enzyme Inhibitors; D008387:Marine Toxins; D052998:Microcystins; D009498:Neurotoxins; D015345:Oligonucleotide Probes; D010456:Peptides, Cyclic; C078588:microcystin