Generation of effector T cells in Hymenolepis nana-infected, FK-506-treated BALB/c mice.
FK-506 administered into mice daily at a dose of 10.0 mg/kg (but not 1.0 and 5.0 mg/kg) caused suppression of protective immunity to Hymenolepis nana, when the agent was injected intraperitoneally during the induction phase of protective immunity. Daily administration of 10.0 mg/kg FK-506, during the course of larval development from challenge, also suppressed protective immunity. Inhibition of protective immunity was only observed in mice that received FK-506 for 6 days at a daily dose of 10.0 mg/kg and were challenged 24 h after the final FK-506 injection. FK-506 did not inhibit formation of effector cells that mediate delayed-type hypersensitivity (DTH) to H. nana egg antigen when the agent was administered intraperitoneally at a dose of 10.0 mg/kg/day for 6 days before cell preparation. However, FK-506 did inhibit DTH effector cell activation when cells prepared from infected, saline-injected mice were transferred into 10.0 mg/kg FK-506-treated recipient mice. These results strongly indicate that FK-506 cannot inhibit the generation of effector cells but will suppress their function in vivo.
Asano
K
K
Department of Medical Biology, School of Medicine, Showa University, Tokyo, Japan.
Matsuo
S
S
Okamoto
K
K
D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D006926:Hymenolepis / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000209:etiology; D007166:Immunosuppressive Agents / Q000008:administration & dosage / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000187:drug effects / Q000469:parasitology*; D016559:Tacrolimus / Q000008:administration & dosage / Q000494:pharmacology*
D007166:Immunosuppressive Agents; D016559:Tacrolimus