title: Functional analysis of sequences adjacent to dapE of Corynebacterium glutamicum reveals the presence of aroP, which encodes the aromatic amino acid transporter.
abstract: An initially nonclonable DNA locus close to a gene of L-lysine biosynthesis in Corynebacterium glutamicum was analyzed in detail. Its stepwise cloning and its functional identification by monitoring the amino acid uptakes of defined mutants, together with mechanistic studies, identified the corresponding structure as aroP, the general aromatic amino acid uptake system.
authors.affiliation: Forschungszentrum Jülich GmbH, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Wehrmann
authors.forename: S
authors.initials: S
authors.lastname: Morakkabati
authors.forename: R
authors.initials: R
authors.lastname: Krämer
authors.forename: H
authors.initials: H
authors.lastname: Sahm
authors.forename: L
authors.initials: L
authors.lastname: Eggeling
mesh_terms: D000595:Amino Acid Sequence; D026905:Amino Acid Transport Systems; D000596:Amino Acids / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010649:Phenylalanine / Q000378:metabolism; D017433:Protein Structure, Secondary; D014364:Tryptophan / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
chemical_list: D026905:Amino Acid Transport Systems; D000596:Amino Acids; C063201:AroP protein, E coli; D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D014443:Tyrosine; D010649:Phenylalanine; D014364:Tryptophan