Expression of membrane-associated C-reactive protein by human monocytes: indications for a selectin-like activity participating in adhesion.
We have shown previously that rat liver macrophages (Kupffer cells) express a membrane-bound form of C-reactive protein (mCRP) on their surface which is identical to a galactose-specific particle receptor activity. We now establish the presence of mCRP on human monocyte-macrophages using immunocytochemistry with an anti-neoCRP specific monoclonal antibody and RNA-RNA in situ hybridization to demonstrate the presence of CRP-specific mRNA. Concomitant with mCRP expression, cells exhibit galactose-dependent uptake of particles coated with lactosylated bovine serum albumin. Adhesion experiments on fibronectin-coated surfaces that mCRP on human blood monocytes may act as a selectin-like adhesion molecule, mediating initial carbohydrate-specific contacts which are followed by peptide-specific recognition via integrin receptors.
Kolb-Bachofen
V
V
Institute for Immunobiology, Medical Faculty, Heinrich-Heine-University Düsseldorf, Germany.
Puchta-Teudt
N
N
Egenhofer
C
C
D000595:Amino Acid Sequence; D002097:C-Reactive Protein / Q000096:biosynthesis* / Q000235:genetics / Q000502:physiology; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000502:physiology*; D004705:Endocytosis; D005353:Fibronectins / Q000378:metabolism; D005690:Galactose / Q000494:pharmacology; D015870:Gene Expression; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D016023:Integrins / Q000378:metabolism; D007700:Kinetics; D007792:Lactulose / Q000494:pharmacology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000378:metabolism / Q000502:physiology*; D009842:Oligopeptides / Q000494:pharmacology
D015815:Cell Adhesion Molecules; D005353:Fibronectins; D016023:Integrins; D009842:Oligopeptides; D007792:Lactulose; D002097:C-Reactive Protein; D005947:Glucose; D005690:Galactose