Use of pulsed-field gel electrophoresis for epidemiological study of Escherichia coli O157:H7 during a food-borne outbreak.
Food and patient isolates from an Escherichia coli O157:H7 outbreak associated with undercooked ground beef were characterized by pulsed-field gel electrophoresis and Shiga-like toxin genotype. Pulsed-field gel electrophoresis confirmed the epidemiologically implicated source of the two-state outbreak and differentiated between outbreak and sporadic strains.
Johnson
J M
JM
Seattle District Laboratory, Food and Drug Administration, Bothell, Washington 98041, USA.
Weagant
S D
SD
Jinneman
K C
KC
Bryant
J L
JL
D001427:Bacterial Toxins / Q000235:genetics; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology*; D005516:Food Microbiology; D006801:Humans; D022641:Shiga Toxin 2
D001427:Bacterial Toxins; D022641:Shiga Toxin 2