Adrenomedullin inhibits the secretion of cytokine-induced neutrophil chemoattractant, a member of the interleukin-8 family, from rat alveolar macrophages.
This study was undertaken to determine the effects of adrenomedullin (AM) on the secretion of cytokine-induced neutrophil chemoattractant (CINC), a member of the interleukin-8 family, from lipopolysaccharide-stimulated rat alveolar macrophages in vitro. AM significantly increased cAMP levels in alveolar macrophages in a dose-dependent fashion. On the other hand, AM significantly inhibited CINC secretion from alveolar macrophages in a dose-dependent fashion, and 8-bromo-cyclic adenosine monophosphate (8-Br-cAMP) also significantly inhibited CINC secretion. These findings suggest that AM may play important roles in the regulation of airway inflammation via a cAMP-dependent mechanism.
Kamoi
H
H
Second Department of Biochemistry, Osaka City University Medical School, Japan.
Kanazawa
H
H
Hirata
K
K
Kurihara
N
N
Yano
Y
Y
Otani
S
S
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D053607:Adrenomedullin; D000818:Animals; D001993:Bronchodilator Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000145:classification / Q000378:metabolism*; D000242:Cyclic AMP / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D006133:Growth Substances / Q000145:classification / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8 / Q000145:classification; D008070:Lipopolysaccharides / Q000494:pharmacology; D016676:Macrophages, Alveolar / Q000187:drug effects*; D008297:Male; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
D001993:Bronchodilator Agents; D019743:Chemokines, CXC; D002630:Chemotactic Factors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D016209:Interleukin-8; D008070:Lipopolysaccharides; D010455:Peptides; D053607:Adrenomedullin; D015124:8-Bromo Cyclic Adenosine Monophosphate; D000242:Cyclic AMP