Expression cloning of a cDNA encoding a fish prolactin receptor.
By using an expression cloning strategy, we isolated a single positive clone encoding a tilapia prolactin (PRL) receptor. Tilapia PRL188 was used to screen a freshwater tilapia kidney expression library transfected in COS cells. The tilapia PRL receptor is a mature protein of 606 amino acids. The extracellular domain is devoid of the tandem repeat units present in birds and has two pairs of cysteine residues, a Trp-Ser-Xaa-Trp-Ser motif, and two potential N-glycosylation sites. The cytoplasmic domain contains 372 amino acids, including box 1, a sequence previously shown to be important for signal transduction in mammalian species. Thus, the general structure is similar to the long form of mammalian PRL receptors; however, amino acid comparisons reveal a rather low identity (approximately 37%). Northern blot analysis shows the existence of a single transcript in osmoregulatory tissues and reproductive organs. This localization is in agreement with known functions of PRL in teleosts.
Sandra
O
O
Unité 344, Endocrinologie Moléculaire, Institut National de la Santé.
Sohm
F
F
de Luze
A
A
Prunet
P
P
Edery
M
M
Kelly
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001717:Birds; D002417:Cattle; D002460:Cell Line; D002645:Chickens; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D010856:Columbidae; D018076:DNA, Complementary / Q000096:biosynthesis / Q000378:metabolism; D015870:Gene Expression; D015723:Gene Library; D006801:Humans; D007668:Kidney; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D011817:Rabbits; D051381:Rats; D011981:Receptors, Prolactin / Q000096:biosynthesis* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D017210:Tilapia; D014162:Transfection
D018076:DNA, Complementary; D012333:RNA, Messenger; D011981:Receptors, Prolactin; D011994:Recombinant Proteins