title: Activin inhibits but inhibin activates mouse placental lactogen-II secretion.
abstract: The regulation of mouse placental lactogen (mPL)-I and mPL-II secretion by activin and inhibin and the expression of activin and inhibin subunit mRNAs in the mouse decidua were examined. Activin-A at a concentration of 10 nM/l significantly inhibited mPL-II secretion by placental cells from days 9 and 12 of pregnancy. However, activin-A did not affect mPL-I secretion by cells from days 7 and 9 of pregnancy nor mPL-II secretion by cells from day 7 of pregnancy. By contrast, 10 nM/l inhibin activated mPL-II secretion by cells from day 12 of pregnancy. These effects of activin and inhibin on mPL-II secretion were dose-dependent. Follistatin, which binds to activin and blocks its bioactivity, completely eliminated the inhibitory effect of activin on mPL-II secretion. Incubation of placental cells from day 12 of pregnancy with activin-A resulted in a significant reduction of the mPL-II mRNA level assessed by Northern blot analysis. Northern blot analysis using poly(A)+RNA extracted from the decidua indicated that mouse decidua, as well as the placenta, express all activin and inhibin subunits and that their gene expressions increased during gestation. The expression of these mRNAs in the decidua was much higher than those in the placenta. These findings suggest that activin and inhibin regulate mPL-II secretion and suggest the presence of an autocrine or paracrine regulation of mPL-II secretion in mouse placenta by activin and inhibin after mid-pregnancy in vivo.
authors.affiliation: Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Tasaka
authors.forename: K
authors.initials: K
authors.lastname: Ogura
authors.forename: M
authors.initials: M
authors.lastname: Sakata
authors.forename: J
authors.initials: J
authors.lastname: Mizuki
authors.forename: A
authors.initials: A
authors.lastname: Miyake
mesh_terms: D028341:Activins; D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D003656:Decidua / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D038681:Follistatin; D015870:Gene Expression; D005865:Gestational Age; D006023:Glycoproteins / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D007265:Inhibins / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D010920:Placenta / Q000187:drug effects / Q000378:metabolism; D010928:Placental Lactogen / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D038681:Follistatin; D006023:Glycoproteins; D006133:Growth Substances; D012333:RNA, Messenger; C062303:placental lactogen II; D028341:Activins; C085699:placental lactogen I, rat; D007265:Inhibins; D010928:Placental Lactogen