Antioxidant and prooxidant roles of copper in Tween 20-induced hemolysis of hamster and pig erythrocytes containing marginal vitamin E.
The concentration-dependent effects of copper acting either as an antioxidant or as a prooxidant were examined in vitro using Tween 20-induced hemolysis. When cupric ion concentration was more than 10 microM, free copper(II) acted as a prooxidant; both extensive hemolysis and production of unknown thiobarbituric acid-reactive substance occurred in hamster and pig erythrocytes irrespective of vitamin E status. However, when cupric ion concentration was 2-4 microM in the incubation medium, copper showed a clear antioxidant activity, reducing both hemolysis and malondialdehyde production induced either by diluted peroxide-containing Tween 20 with ascorbic acid and sodium azide in vitamin E-deficient hamster erythrocytes, or by peroxide-containing Tween 20 in pig erythrocytes containing marginal amounts of vitamin E. Copper(II) is taken up by the erythrocytes, where copper(I)-complexes may contribute to the protection of cells with membrane vitamin E against oxidative radical attack.
Hamada
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Laboratory of Trace Elements Nutrition, National Institute of Animal Industry, Ibaraki, Japan.
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003300:Copper / Q000494:pharmacology*; D006224:Cricetinae; D004032:Diet; D004912:Erythrocytes / Q000502:physiology*; D006461:Hemolysis / Q000187:drug effects; D008297:Male; D016877:Oxidants / Q000378:metabolism; D011136:Polysorbates / Q000494:pharmacology; D013552:Swine; D014810:Vitamin E / Q000008:administration & dosage / Q000378:metabolism*; D014811:Vitamin E Deficiency / Q000378:metabolism
D000975:Antioxidants; D016877:Oxidants; D011136:Polysorbates; D014810:Vitamin E; D003300:Copper