Overproduction of His-tagged EcoRII restriction endonuclease and terminally deleted mutant proteins.
EcoRII was the first restriction endonuclease (ENase) reported requiring the cooperative interaction with at least two DNA sites for activity. Using two different expression systems the enzyme could be purified and its special substrate requirements were further analyzed. At the present state of knowledge we suggest a model of simultaneous binding of two DNA sites to one dimeric enzyme molecule (see Krüger et al., FEMS Microbiol. Rev. (1995) in press).
Kupper
D
D
Institute of Medical Virology, Humboldt University School of Medicine (Charité), Berlin, Germany.
Reuter
M
M
Krüger
D H
DH
D001665:Binding Sites; D004247:DNA / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000096:biosynthesis* / Q000302:isolation & purification / Q000378:metabolism; D015870:Gene Expression; D006639:Histidine; D016296:Mutagenesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D017384:Sequence Deletion; D016324:Sequence Tagged Sites; D013379:Substrate Specificity
D011994:Recombinant Proteins; D006639:Histidine; D004247:DNA; C046545:CCWGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific