Murine tumorlytic factor, immunologically distinct from tumor necrosis factor-alpha and -beta, induced in the serum of mice treated with a T-cell mitogen of Corynebacterium kutscheri.
Murine tumorlytic factor (TF), immunologically distinct from murine tumor necrosis factor (TNF)-alpha and -beta, was purified to a homogeneity from the serum of mice injected with a T-cell mitogen of Corynebacterium kutscheri. The treated mouse serum was purified by Lentil lectin-Sepharose chromatography, DEAE-cellulose chromatography, preparative isoelectric focusing, and high-pressure liquid chromatography to the specific activity of 1.5 x 10(6) U/mg protein. TF was 42 kDa in its oligomeric form and 14 kDa in its monomeric form. TF activity was not impaired with hamster monoclonal antibody (mAb) to recombinant murine TNF-alpha and -beta and, reciprocally, rabbit antibody to TF neutralized the bioactivity of neither murine TNF-alpha nor -beta. TF was not precipitated with the mAb to murine TNF-alpha and -beta in Western blot analysis. The partial amino acid sequence of TF was at most 33% homologous to the 46-63 sequence of mouse TNF-beta. Thus, these results suggest that TF might be a novel tumorlytic factor which is immunologically distinct from mouse TNF-alpha and -beta.
Kita
E
E
Department of Bacteriology, Nara Medical University, Japan.
Matsui
N
N
Sawaki
M
M
Mikasa
K
K
Katsui
N
N
D000595:Amino Acid Sequence; D000818:Animals; D003352:Corynebacterium; D003603:Cytotoxins / Q000096:biosynthesis / Q000097:blood* / Q000302:isolation & purification; D005260:Female; D008233:Lymphotoxin-alpha / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification
D003603:Cytotoxins; D008233:Lymphotoxin-alpha; D008934:Mitogens; D014409:Tumor Necrosis Factor-alpha; C096504:serum tumorlytic factor