Monoclonal antibodies against camel (Camelus dromedarius) IgG, IgM and light chains.
Monoclonal antibodies specific for camel IgG and IgM heavy chains and immunoglobulin light chains were produced by a simple, time-saving and efficient method. Popliteal lymph nodes isolated 9 days after a primary foot-pad immunisation were used as the source of antibody producing hybridoma cells. Ascites was induced in mice and ascitic fluid collected. The specificity of anti-IgG and anti-IgM monoclonal antibodies was determined by enzyme-linked immunosorbent assay (ELISA) and Western blotting. Monoclonal antibodies specifically reacting to IgG1 subclasses were demonstrated; no monoclonal antibodies specific to IgG2 or IgG3 were generated. An unexpected finding was that some class-specific monoclonal antibodies were light chain, and not heavy chain, reactive.
Azwai
S M
SM
Department of Veterinary Pathology, University of Liverpool, UK.
Carter
S D
SD
Woldehiwet
Z
Z
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D002162:Camelus / Q000276:immunology*; D002459:Cell Fusion; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006825:Hybridomas / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains / Q000276:immunology; D007147:Immunoglobulin Light Chains / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M