title: Glutamine supplementation prevents the decrease of mitogen response after a treadmill exercise in rats.
abstract: The effect of glutamine (Gln)-supplemented diet on mitogen response decreased immediately after a treadmill exercise was examined by measuring the proliferations of peripheral blood lymphocytes (PBL) with phytohemagglutinin (PHA) and concanavalin A (ConA) in male Fisher rats. Although the plasma Gln concentration was significantly decreased in the control group immediately after a treadmill exercise (20 m/min, 60 min) compared to rested rats, plasma Gln concentration of rats fed Gln-supplemented diet for 3 weeks was significantly higher than that of control group in resting and was not significantly decreased even immediately after a treadmill exercise. In addition, proliferation of PBL with PHA or ConA and interleukin 2 (IL2) production were also significantly decreased immediately after a treadmill exercise in control group. On the contrary, their functions were almost maintained in Gln-supplemented group even immediately after a treadmill exercise. PBL from rats fed Gln-supplemented diet showed a higher response to mitogens such as PHA and ConA compared to the control group. Furthermore, their PBL showed higher incorporation of [3H]Gln compared to that of the control group irrespective of treadmill exercise. These results indicate that the preventive effect of Gln-supplemented diet on mitogen response decreased after a treadmill exercise is due to an increased response to mitogen and increased uptake of Gln as sources of fuel and nucleotides to the immune cells.
authors.affiliation: Department of Nutrition, School of Medicine, University of Tokushima, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Moriguchi
authors.forename: H
authors.initials: H
authors.lastname: Miwa
authors.forename: Y
authors.initials: Y
authors.lastname: Kishino
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology; D003208:Concanavalin A / Q000494:pharmacology; D004435:Eating / Q000502:physiology; D005080:Exercise Test; D005527:Food, Fortified; D005973:Glutamine / Q000097:blood / Q000378:metabolism / Q000494:pharmacology*; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D005082:Physical Exertion / Q000502:physiology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014316:Tritium
chemical_list: D007376:Interleukin-2; D010835:Phytohemagglutinins; D005973:Glutamine; D014316:Tritium; D003208:Concanavalin A