Cloning and chromosomal localization of the human ileal lipid-binding protein.
A human ileal lipid-binding protein (ILBP3) cDNA was isolated and shown to encode a 128 amino acid protein with homology to the fatty acid-binding protein gene family. The human ILBP amino acid sequence exhibited 78% identity to the rat ILBP sequence. Northern blot analysis revealed a single transcript of approx. 0.6 kb in small intestine, but no hybridization to liver, spleen, thymus, prostate, ovary, caecum, or colon. Southern blot analysis of genomic DNA from a panel of rodent-human somatic cell hybrids revealed that the human ILBP gene resides on chromosome 5 and is not linked to any other known fatty acid-binding protein family member.
Oelkers
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Department of Internal Medicine-Gastroenterology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157, USA.
Dawson
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PA
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D002895:Chromosomes, Human, Pair 5; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D006801:Humans; D006822:Hybrid Cells; D007082:Ileum / Q000378:metabolism* / Q000648:ultrastructure; D008969:Molecular Sequence Data; D029363:Organic Anion Transporters, Sodium-Dependent; D016415:Sequence Alignment; D027981:Symporters
D002352:Carrier Proteins; D018076:DNA, Complementary; D029363:Organic Anion Transporters, Sodium-Dependent; D027981:Symporters; C071908:sodium-bile acid cotransporter