[Relationship between cellular DNA contents and cell proliferation of non-small cell lung cancer estimated by simultaneous quantification of PCNA and DNA contents by flow cytometer].
Cellular DNA contents and proliferating cell nuclear antigen (PCNA) expression were studied in 273 fresh specimens from 65 surgically resected non-small cell lung cancers (adenocarcinoma 36, squamous cell carcinoma 29), and the relationship between the cellular DNA contents, especially the DNA ploidy pattern, and the cell proliferation was evaluated. The cellular DNA content and PCNA labeling index (LI) % were assayed with flow cytometry using simultaneous double staining technique. The cells of G0/G1 phase of cell cycle were classified into two categories by the DNA index (DI); diploid cells (DI = 1.0) and aneuploid cells (DI > 1.0). PCNA LI % was measured at all phase and G0/G1 phase of cell cycle. PCNA LI % of diploid tumors was significantly higher than that of normal lung tissues (p < 0.05), and PCNA LI % of aneuploid tumors was significantly higher than that of diploid tumors (p < 0.05). PCNA LI % of diploid cells of tumors was significantly higher than that of diploid cells of normal lung tissues (p < 0.05), and PCNA LI % of aneuploid cells of tumors was significantly higher than that of diploid cells of tumors (p < 0.05). In adenocarcinomas, % aneuploid was directly correlated to PCNA LI % at all phase. From this result, it was concluded that DNA aneuploid tumors have a high cell proliferative pattern when the % aneuploid is high in DNA histogram.
Morita
K
K
First Dept. of Surgery, Kanazawa University School of Medicine.
Hayashi
Y
Y
Nagao
S
S
Watanabe
Y
Y
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D002455:Cell Division; D004273:DNA, Neoplasm / Q000032:analysis* / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D011003:Ploidies; D011379:Prognosis; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*
D004273:DNA, Neoplasm; D018809:Proliferating Cell Nuclear Antigen