An autologous fibrin tissue adhesive with greater bonding power.
OBJECTIVE
To describe and evaluate an autologous fibrin tissue adhesive (AFTA) that uses a combination of ethanol and freezing to precipitate fibrinogen (AFTA-E).


METHODS
The bonding power of AFTA-E was compared with that of a conventional AFTA based on ammonium sulfate precipitation of fibrinogen (AFTA-A). In this study, Silastic, porcine dermis, and human dura mater blocks were bonded together for 10 and 30 minutes with AFTA-E or AFTA-A. The blocks were then separated and the bonding power was measured. The efficacy of AFTA-E was also evaluated after a 24-hour refrigeration.


METHODS
The Department of Otolaryngology Research Laboratory at the University of Illinois Eye and Ear Infirmary, Chicago, Ill.


METHODS
Blood was drawn from 86 healthy volunteers and AFTA-E and/or AFTA-A was manufactured.


RESULTS
The AFTA-E was shown statistically to bond stronger than the AFTA-A. In addition, it was found that the efficacy of AFTA-E was unchanged after a 24-hour refrigeration.


CONCLUSIONS
The improved AFTA, AFTA-E, is a superior alternative to the conventional AFTA-A. Furthermore, AFTA-E can be manufactured before surgery and stored, thus minimizing preparation time during surgery.
Siedentop
K H
KH
Department of Otolaryngology-Head and Neck Surgery, University of Illinois, College of Medicine, Chicago, USA.
Park
J J
JJ
Sanchez
B
B
D000268:Adhesiveness; D000645:Ammonium Sulfate; D000818:Animals; D003487:Cyanoacrylates / Q000494:pharmacology; D004388:Dura Mater / Q000187:drug effects; D000431:Ethanol; D015718:Fibrin Tissue Adhesive / Q000302:isolation & purification / Q000494:pharmacology*; D005615:Freezing; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing / Q000379:methods; D012826:Silicone Elastomers; D012867:Skin / Q000187:drug effects; D013552:Swine; D013997:Time Factors
D003487:Cyanoacrylates; D015718:Fibrin Tissue Adhesive; D012826:Silicone Elastomers; D000431:Ethanol; D000645:Ammonium Sulfate