Isolation of porcine adenovirus as a candidate of 5th serotype.
A strain of cytopathic virus, named strain TG/K79, was isolated from the brain of a newborn piglet, pure Hampshire breed, which died shortly after birth. The physicochemical property of virus was considered to be that of the family Adenoviridae. A significant difference between our isolated and 4 reference porcine adenoviruses was demonstrated by cross-seroneutralization test. Differences between TG/K79 and other porcine adenoviruses were also seen in electrophoretic patterns of viral DNA in agarose gel after digested by restriction endonucleases. Two SPF pigs, 2-month-old, experimentally infected via intranasal showed a fever and a hemorrhagic enteritis. A serological survey indicates that at least swine in the farm where the virus was isolated have been highly contaminated.
Kadoi
K
K
National Veterinary Assay Laboratory for Biological Products, Ministry of Agriculture, Tokyo, Japan.
Inoue
Y
Y
Ikeda
T
T
Kamata
H
H
Yukawa
M
M
Iwabuchi
M
M
Inaba
Y
Y
D000257:Adenoviridae Infections / Q000662:veterinary* / Q000821:virology; D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D001921:Brain / Q000821:virology*; D016172:DNA Fingerprinting; D004279:DNA, Viral / Q000032:analysis; D004751:Enteritis / Q000662:veterinary / Q000821:virology; D005260:Female; D008297:Male; D017921:Mastadenovirus / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D014771:Virion / Q000648:ultrastructure
D000914:Antibodies, Viral; D004279:DNA, Viral