Coordinate regulation of rat renal parathyroid hormone receptor mRNA and Na-Pi cotransporter mRNA and protein.
Parathyroid hormone (PTH) acts on the kidney by binding to the PTH receptor, leading to a decrease in the active renal reabsorption of phosphate by the Na-Pi cotransporter, which is also independently activated by hypophosphatemia. We have studied the effects of hypo- and hyperparathyroidism and hypophosphatemia on PTH receptor mRNA and Na-Pi cotransporter mRNA and protein. Both surgical parathyroidectomy and hypophosphatemia, which itself leads to hypoparathyroidism, led to an upregulation of the PTH receptor mRNA and Na-Pi cotransporter mRNA and protein. Parathyroidectomized rats fed a low-Pi diet had an increase in PTH receptor and Na-Pi cotransporter mRNAs. Diet-induced hyperparathyroidism had no effect on PTH receptor mRNA and Na-Pi cotransporter mRNA and protein. The effect of hypoparathyroidism and hypophosphatemia to increase both PTH receptor mRNA and Na-Pi cotransporter mRNA and protein shows that there is a tight coordinate regulation of these factors, which are both involved in PTH action.
Kilav
R
R
Minerva Center for Calcium and Bone Metabolism, Hadassah University Hospital, Hebrew University Hadassah Medical School, Jerusalem, Israel.
Silver
J
J
Biber
J
J
Murer
H
H
Naveh-Many
T
T
D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000096:biosynthesis*; D005786:Gene Expression Regulation; D006961:Hyperparathyroidism / Q000378:metabolism*; D007011:Hypoparathyroidism / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D008297:Male; D016105:Parathyroidectomy; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D018016:Receptors, Parathyroid Hormone / Q000096:biosynthesis*; D012016:Reference Values; D050608:Sodium-Phosphate Cotransporter Proteins; D027981:Symporters
D002352:Carrier Proteins; D012333:RNA, Messenger; D018016:Receptors, Parathyroid Hormone; D050608:Sodium-Phosphate Cotransporter Proteins; D027981:Symporters