Molecular cloning of human liver sulfite oxidase.
A 2.4 kilobase cDNA clone of human sulfite oxidase was isolated from a human liver cDNA library in lambda gt10. Comparison of three sulfite oxidase sequences to several plant and fungal nitrate reductase sequences reveals a single conserved cysteine with highly conserved flanking sequences. The conserved cysteine is postulated to be a ligand of molybdenum in sulfite oxidase and nitrate reductase.
Garrett
R M
RM
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Bellissimo
D B
DB
Rajagopalan
K V
KV
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000302:isolation & purification*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis
D018076:DNA, Complementary; D011994:Recombinant Proteins; D050862:Oxidoreductases Acting on Sulfur Group Donors