Phage-mediated conjugation in staphylococci as a means of transfer of drug-resistance.
Penicillin resistance plasmid was transferred from Staphylococcus aureus B4 (PcrKms, donor) to S. aureus ML351 (PcsKmr, recipient) by co-cultivation of the donor with the recipient in nutrient broth with or without the modifying effects of CaCl2 or sodium dodecyl sulfate. It was found that the transfer of drug-resistance occurred maximally between 6 and 18 hr postinoculation; however, addition of DNase (200 micrograms/ml) could totally prevent such a transfer up to 6 hr and significantly reduce it thereafter. Cell-free filtrate of the donor culture when mixed with the recipient was ineffective in bringing about the transfer of Pcr.
Mitra
M
M
Department of Medical Microbiology and Parasitology, Calcutta University College of Medicine, India.
Mukherjee
M
M
Gupta
M S
MS
Chakrabarty
A N
AN
Dastidar
S G
SG
D003227:Conjugation, Genetic; D018014:Gene Transfer Techniques; D007613:Kanamycin Resistance / Q000235:genetics*; D010403:Penicillin Resistance / Q000235:genetics*; D010957:Plasmids; D013204:Staphylococcus Phages / Q000235:genetics*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*