title: Comparison of a direct antigen enzyme immunoassay, Herpchek, with cell culture for detection of herpes simplex virus from clinical specimens.
abstract: Direct enzyme immunoassay (Herpchek) was compared with culture on 21,522 specimens mainly from asymptomatic women for herpes simplex virus detection. Sensitivity and specificity were 73.8 and 97.7%, respectively. The 33% detection rate by enzyme immunoassay in 5 h increased to 43% when the enzyme immunoassay was combined with culture. Herpchek alone was not sensitive enough, but in combination with culture, maximum detection was achieved.
authors.affiliation: Diagnostic Virology Laboratory, Corning Clinical Laboratory, Teterboro, New Jersey 07608, USA.
authors.forename: L
authors.initials: L
authors.lastname: Verano
authors.forename: F J
authors.initials: FJ
authors.lastname: Michalski
mesh_terms: D000293:Adolescent; D000328:Adult; D000956:Antigens, Viral; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005260:Female; D006558:Herpes Genitalis / Q000150:complications / Q000175:diagnosis / Q000821:virology; D006561:Herpes Simplex / Q000150:complications / Q000175:diagnosis / Q000821:virology; D018259:Herpesvirus 1, Human / Q000276:immunology / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007124:Immunoenzyme Techniques / Q000706:statistics & numerical data; D008297:Male; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis / Q000821:virology; D012680:Sensitivity and Specificity; D018139:Simplexvirus / Q000276:immunology / Q000302:isolation & purification*; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data; D014776:Virus Cultivation / Q000379:methods / Q000706:statistics & numerical data
chemical_list: D000956:Antigens, Viral