title: The gamma-aminobutyric acid receptor gamma 3 subunit gene (GABRG3) is tightly linked to the alpha 5 subunit gene (GABRA5) on human chromosome 15q11-q13 and is transcribed in the same orientation.
abstract: GABAA receptors are heterooligomeric ligand-gated ion channels that mediate the effect of the inhibitory neurotransmitter gamma-aminobutyric acid. The GABAA receptors consist of at least 15 different receptor subunits that can be classified into 5 subfamilies (alpha, beta, gamma, delta, rho) on the basis of sequence similarity. Chromosomal mapping studies have revealed that several of the GABAA receptor subunit genes appear to be organized as clusters. One such cluster, which consists of the GABAA receptor beta 3 (GABRB3) and alpha 5 (GABRA5) subunit genes, is located in chromosome 15q11-q13. It is shown here that the GABAA receptor gamma 3 subunit gene (GABRG3) also maps to this region. Lambda and P1 phage clones surrounding both ends of GABRG3 were isolated; the clones derived from the 5' end of GABRG3 were linked to an existing phage contig spanning the 3' end of GABRA5. The two genes are located within 35 kb of each other and are transcribed in the same orientation.
authors.affiliation: Genetics Division, Children's Hospital, Boston, MA 02115, USA.
authors.forename: V
authors.initials: V
authors.lastname: Greger
authors.forename: J H
authors.initials: JH
authors.lastname: Knoll
authors.forename: E
authors.initials: E
authors.lastname: Woolf
authors.forename: K
authors.initials: K
authors.lastname: Glatt
authors.forename: R F
authors.initials: RF
authors.lastname: Tyndale
authors.forename: T M
authors.initials: TM
authors.lastname: DeLorey
authors.forename: R W
authors.initials: RW
authors.lastname: Olsen
authors.forename: A J
authors.initials: AJ
authors.lastname: Tobin
authors.forename: J M
authors.initials: JM
authors.lastname: Sikela
authors.forename: Y
authors.initials: Y
authors.lastname: Nakatsu
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002884:Chromosomes, Human, Pair 15; D003001:Cloning, Molecular; D015870:Gene Expression; D005796:Genes; D008040:Genetic Linkage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D011963:Receptors, GABA-A / Q000737:chemistry / Q000235:genetics*
chemical_list: D011963:Receptors, GABA-A