Confirmation of the identity of human skeletal remains using Multiplex PCR Amplification and Typing Kits.
The identify of human skeletal remains found in a wooded area approximately one year after the person was reported missing was provisionally established by routine methods and circumstantial evidence. Multiplex PCR systems--the AmpliType PM PCR Amplification and Typing Kit and the GenePrint STR Triplex Amplification and Typing Kit--were used to confirm the identification. DNA profiles from femur bone from the remains were compared with profiles derived from head hairs from a hairbrush recovered in the missing woman's apartment. In addition, a sex typing procedure using the X-Y homologous gene amelogenin was carried out. This is the first report of a case using commercially available multiplex PCR amplification and typing kits to confirm the identity of skeletal remains.
Hochmeister
M N
MN
DNA Identity Laboratory, University of Berne, Switzerland.
Budowle
B
B
Borer
U V
UV
Rudin
O
O
Bohnert
M
M
Dirnhofer
R
R
D001842:Bone and Bones; D004247:DNA / Q000032:analysis*; D018732:Forensic Anthropology; D006197:Hair; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods
D004247:DNA