title: Identification of major proteins associated with Dictyostelium discoideum endocytic vesicles.
abstract: Magnetic isolation of endocytic vesicles from Dictyostelium discoideum was accomplished after feeding the amoebae with iron oxide particles. Proteins associated with the endocytic vesicles were resolved by SDS-PAGE and digested 'in-gel' with endoproteinase Lys-C or Asp-N to generate peptides for amino acid sequencing. This strategy allowed the identification of the major protein constituents of the vesicles: namely, the A, B, D, E and 110 kDa subunits of a vacuolar type H(+)-ATPase, actin, a Rab 7-like GTPase, a p34 protein corresponding to a new cysteine proteinase and the 25 kDa product of a recently sequenced D. discoideum open reading frame.
authors.affiliation: Laboratoire de Chimie des Protéines, CEA, Centre d'Etudes Nucléaires, Grenoble, France.
authors.forename: C
authors.initials: C
authors.lastname: Adessi
authors.forename: A
authors.initials: A
authors.lastname: Chapel
authors.forename: M
authors.initials: M
authors.lastname: Vinçon
authors.forename: T
authors.initials: T
authors.lastname: Rabilloud
authors.forename: G
authors.initials: G
authors.lastname: Klein
authors.forename: M
authors.initials: M
authors.lastname: Satre
authors.forename: J
authors.initials: J
authors.lastname: Garin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003594:Cytoplasmic Granules / Q000378:metabolism; D004023:Dictyostelium / Q000166:cytology / Q000378:metabolism*; D004705:Endocytosis; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D011506:Proteins