[Simultaneous measurement of unsaturated fatty acid and lipid peroxide in vivo by high performance liquid chromatography].
A chemiluminescence high performance liquid chromatography (HPLC) method for the simultaneous measurement of unsaturated fatty acid and lipid peroxide is newly described. This method was developed using reversed-phase HPLC which could separate unsaturated fatty acid and lipid peroxide clearly. Linear relationship was observed between these lipid peroxides and their peak areas on the chromatogram. The hydroperoxides were recently suggested to cause some diseases such as gastric lesions due to stress, but convenient method has not been established. The most popular method is the thiobarbituric acid (TBA) test, which has been extensively modified during the last several year. But the major disadvantage in this method is the interference of other thiobarbituric acid reactive substances (TBARS) and it is uncertain whether TBARS reflects the hydroperoxide concentration. This method is a highly sensitive fluorometric method for the quantitation of hydroperoxide at the nanomole level. Chemiluminescence was produced through luminol oxidation during the reaction of the hydroperoxide and cytochrome cheme. It is concluded that reversed phase HPLC can complement each other to serve as a direct and sensitive method for the determination of unsaturated fatty acid and lipid peroxide levels in vivo.
Ohisa
Y
Y
Department of Clinical & Laboratory Medicine, Tohoku University School of Medicine, Sendai.
Kawamura
T
T
Mizonobe
K
K
Funato
T
T
Sasaki
T
T
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005231:Fatty Acids, Unsaturated / Q000032:analysis*; D008054:Lipid Peroxides / Q000032:analysis*; D008163:Luminescent Measurements; D051381:Rats
D005231:Fatty Acids, Unsaturated; D008054:Lipid Peroxides