Sequence identity of the n-1 product of a synthetic oligonucleotide.
After synthesis and purification of an oligonucleotide, the final product usually contains a low level of n-1 congeneric species. We have sequenced the n-1 population of a 25mer phosphodiester oligonucleotide. The n-1 band was cut from the gel and eluted. Oligonucleotides were tailed with dA and annealed to a dT-tailed plasmid. The recombinant plasmid was ligated and used to transform competent bacteria. Our results show that the n-1 population was heterogeneous. The frequency of truncated nucleotides at the 3'-end was much higher than at the 5'-end of the oligomer. No truncated nucleotides were found in the last four nucleotides at the 5'-end. Our results also show that the chain of oligonucleotides can grow on unreacted sites of a controlled-pore glass support.
Temsamani
J
J
Hybridon, Inc., Worcester, MA 01605, USA.
Kubert
M
M
Agrawal
S
S
D001483:Base Sequence; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D017421:Sequence Analysis
D009841:Oligonucleotides