title: Overexpression in Escherichia coli of 12 vitamin B12 biosynthetic enzymes.
abstract: The first 12 enzymes involved in the biosynthesis of vitamin B12 from its five-carbon precursor, aminolevulinic acid, have been overexpressed in recombinant strains of Escherichia coli. The activity of each enzyme has been demonstrated by the biosynthesis of hydrogenobyrinic acid from aminolevulinic acid.
authors.affiliation: Center for Biological NMR, Department of Chemistry, Texas A&M University, College Station 77843-3255, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Roessner
authors.forename: J B
authors.initials: JB
authors.lastname: Spencer
authors.forename: S
authors.initials: S
authors.lastname: Ozaki
authors.forename: C
authors.initials: C
authors.lastname: Min
authors.forename: B P
authors.initials: BP
authors.lastname: Atshaves
authors.forename: P
authors.initials: P
authors.lastname: Nayar
authors.forename: N
authors.initials: N
authors.lastname: Anousis
authors.forename: N J
authors.initials: NJ
authors.lastname: Stolowich
authors.forename: M T
authors.initials: MT
authors.lastname: Holderman
authors.forename: A I
authors.initials: AI
authors.lastname: Scott
mesh_terms: D000622:Aminolevulinic Acid / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D015870:Gene Expression; D005798:Genes, Bacterial; D005822:Genetic Vectors; D006801:Humans; D011163:Hydroxymethylbilane Synthase / Q000096:biosynthesis / Q000235:genetics; D019751:Intramolecular Transferases; D007535:Isomerases / Q000096:biosynthesis / Q000235:genetics; D008780:Methyltransferases / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000096:biosynthesis / Q000235:genetics; D010105:Oxygenases / Q000096:biosynthesis / Q000235:genetics; D000623:Porphobilinogen Synthase / Q000096:biosynthesis / Q000235:genetics; D011549:Pseudomonas / Q000201:enzymology / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000378:metabolism; D014576:Uroporphyrinogen III Synthetase / Q000096:biosynthesis / Q000235:genetics; D014578:Uroporphyrins / Q000096:biosynthesis*; D014805:Vitamin B 12 / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; D014578:Uroporphyrins; C078447:precorrin 3; C072476:hydrogenobyrinic acid; D000622:Aminolevulinic Acid; D010088:Oxidoreductases; D010105:Oxygenases; C083406:CobG protein, Pseudomonas denitrificans; C094566:precorrin-6 reductase; C490608:precorrin-6 reductase, E coli; D008780:Methyltransferases; C066231:precorrin-2 O-methyltransferase; C094568:precorrin-4 methyltransferase, E coli; C094570:precorrin-5 methyltransferase, bacteria; C094567:precorrin-6 decarboxylase, E coli; C060508:uroporphyrin-III C-methyltransferase; D011163:Hydroxymethylbilane Synthase; D000623:Porphobilinogen Synthase; D014576:Uroporphyrinogen III Synthetase; D007535:Isomerases; D019751:Intramolecular Transferases; C072474:CobH protein, Pseudomonas denitrificans; D014805:Vitamin B 12