Cell culture-PCR technique for detection of infectious cytomegalovirus in peripheral blood.
We determined the efficacy of PCR for the direct detection of infectious cytomegalovirus (CMV) in specimen-inoculated MRC-5 tube cultures (cell culture-PCR [CC-PCR]). Parallel testing was performed by a shell vial assay-indirect immunofluorescence assay (SVA-IFA) and isolation (conventional MRC tube cultures [TC-CPE]. The sensitivity, specificity, and positive and negative predictive values for CC-PCR, SVA-IFA, and TC-CPE were 81, 99, 94, and 94%, 86, 100, 100, and 96%, and 77, 100, 100, and 93%, respectively. Future application of CC-PCR may be directed toward its use as a screening tool for cytomegalovirus genotypic variants.
Lipson
S M
SM
Jane and Dayton Brown and Dayton T. Brown Jr., Virology Laboratory, Department of Medicine, North Shore University Hospital, Manhasset, New York 11030, USA.
Ashraf
A B
AB
Lee
S H
SH
Kaplan
M H
MH
Shepp
D H
DH
D001769:Blood / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis / Q000821:virology; D003588:Cytopathogenic Effect, Viral; D003951:Diagnostic Errors; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique / Q000706:statistics & numerical data; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D014766:Viremia / Q000175:diagnosis / Q000821:virology; D014773:Virology / Q000379:methods* / Q000706:statistics & numerical data