Nucleotide-selective cleavage of duplex DNA by nitric oxide.
In the presence of sodium nitrite and sodium hydrosulfite, the produced NO has been confirmed by the detection of bleomycin-Fe(II)-NO adduct complex. Nitric oxide clearly induces DNA form change of form I-->forms II & III. Of special interest is the fact that (1) NO attacks guanine bases as the most preferential cleaving sites and (2) the frequency of the NO-induced cutting decreases in the order G > C > A > T. The preferred cleavage at GC sequences by NO may correlate to predominant mutagenicity at GC sites by nitric oxide.
Sugiura
Y
Y
Institute for Chemical Research, Kyoto University, Japan.
Matsumoto
T
T
D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000187:drug effects; D018736:DNA Adducts; D004249:DNA Damage; D004278:DNA, Superhelical / Q000737:chemistry / Q000187:drug effects; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D008969:Molecular Sequence Data; D009153:Mutagens / Q000737:chemistry* / Q000494:pharmacology; D009569:Nitric Oxide / Q000737:chemistry* / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D017422:Sequence Analysis, DNA
D018736:DNA Adducts; D004278:DNA, Superhelical; D009153:Mutagens; D009569:Nitric Oxide; D004247:DNA; D015252:Deoxyribonucleases, Type II Site-Specific