title: Specific interaction of Escherichia coli O157:H7-derived Shiga-like toxin II with human renal endothelial cells.
abstract: In Escherichia coli O157:H7 foodborne infections of humans, the Shiga-like toxins (SLTs) are thought to be the cause of life-threatening vascular complications, including acute renal disease known as hemolytic uremic syndrome or HUS. As virtually all E. coli O157:H7 isolates from HUS patients produce SLT-II (vs. SLT-I), the possible preferential interaction of SLT-II with human renal microvascular endothelial cells (HRMEC), the putative target of the SLTs in the development of HUS, was studied. SLT-II was 1000 times more potent a cytotoxic agent than SLT-I toward HRMEC. Toxin binding studies showed that this occurred although HRMEC could bind 10 times more SLT-I than SLT-II. This preferential action of SLT-II was specific for renal endothelial cells, as human umbilical vein endothelial cells were almost equally affected by SLT-I and SLT-II.
authors.affiliation: Department of Microbiology and Immunology, University of Rochester School of Medicine & Dentistry, New York 14642, USA.
authors.forename: C B
authors.initials: CB
authors.lastname: Louise
authors.forename: T G
authors.initials: TG
authors.lastname: Obrig
mesh_terms: D001427:Bacterial Toxins / Q000378:metabolism* / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D004768:Enterotoxins / Q000633:toxicity*; D004926:Escherichia coli; D006017:Glycolipids / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008833:Microcirculation; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012079:Renal Circulation; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; D014470:Umbilical Cord
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D006017:Glycolipids; D011956:Receptors, Cell Surface; D022622:Shiga Toxin 1; D022641:Shiga Toxin 2; C068556:Shiga-like toxin receptor