title: Improved PCR method for high-efficiency site-directed mutagenesis using class 2S restriction enzymes.
authors.affiliation: University of Brussels, Nivelles, Belgium.
authors.forename: J P
authors.initials: JP
authors.lastname: Vilardaga
authors.forename: E
authors.initials: E
authors.lastname: Di Paolo
authors.forename: A
authors.initials: A
authors.lastname: Bollen
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D015252:Deoxyribonucleases, Type II Site-Specific