title: Amplification of DAT1 (human dopamine transporter gene) 3' variable region in the Japanese population.
abstract: Amplification of the human dopamine transporter gene (DAT1) was performed by the polymerase chain reaction, and polymorphism of the VNTR locus was analyzed in a population sample of 176 randomly selected Japanese individuals. Genomic DNAs were extracted from whole blood, and the 3' untranslated region of the human DAT1 gene was amplified. The amplification products were distinguished by agarose gel electrophoresis, and visualized directly by ethidium bromide staining. In this way, four alleles were visualized directly and designated A, B, C, and D in decreasing order according to size. The relative frequencies of DAT1 allels A through D were 0.0171, 0.0625, 0.9119, and 0.0085, respectively. Evidence of significant deviations from the Hardy-Weinberg equilibrium was observed from these Japanese population data. The DAT1 locus was estimated to have a heterozygosity index of 13% and polymorphic information content of 0.16.
authors.affiliation: Department of Legal Medicine, Osaka University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nakatome
authors.forename: K
authors.initials: K
authors.lastname: Honda
authors.forename: M N
authors.initials: MN
authors.lastname: Islam
authors.forename: M
authors.initials: M
authors.lastname: Terada
authors.forename: M
authors.initials: M
authors.lastname: Yamazaki
authors.forename: H
authors.initials: H
authors.lastname: Kuroki
authors.forename: Y
authors.initials: Y
authors.lastname: Ogura
authors.forename: H
authors.initials: H
authors.lastname: Bai
authors.forename: C
authors.initials: C
authors.lastname: Wakasugi
mesh_terms: D000483:Alleles; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D004247:DNA / Q000235:genetics; D017931:DNA Primers; D004298:Dopamine / Q000378:metabolism; D050483:Dopamine Plasma Membrane Transport Proteins; D005787:Gene Frequency; D014644:Genetic Variation; D006801:Humans; D007564:Japan; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: D002352:Carrier Proteins; D017931:DNA Primers; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; C493214:SLC6A3 protein, human; D004247:DNA; D004298:Dopamine