Lipopolysaccharide-associated elevation of serum and urinary nitrite/nitrate levels and hematological changes in rats.
The objective of this study was to assess the relationship between lipopolysaccharide (LPS)-induced elevation of nitric oxide (NO) levels and hematological changes. Twenty-four h following i.p. treatment of LPS (1 mg/kg body wt.), nitrite/nitrate (NO2-/NO3-) levels in the serum and urine of rats were, respectively, increased to 11 and 50 times those of control. Time-dependent decrease of white blood cells (80% of control), lymphocytes (40% of control), and platelets (35% of control) was also observed, while a significant increase of neutrophils (330% of control) and monocytes (650% of control) occurred during the 24-h post-treatment period. These results suggest that LPS-induced increase of NO2-/NO3- levels and coincident hematological changes may compromise immune functions.
Kitajima
S
S
Division of Toxicology, National Institute of Health Sciences, Tokyo, Japan.
Tsuda
M
M
Eshita
N
N
Matsushima
Y
Y
Saitoh
M
M
Momma
J
J
Kurokawa
Y
Y
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects; D004926:Escherichia coli; D007274:Injections, Intraperitoneal; D007958:Leukocyte Count / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D009566:Nitrates / Q000097:blood* / Q000652:urine; D009573:Nitrites / Q000097:blood* / Q000652:urine; D051381:Rats; D017208:Rats, Wistar
D008070:Lipopolysaccharides; D009566:Nitrates; D009573:Nitrites