Onchocerca volvulus larval antigen, OvB20, induces partial protection in a rodent model of onchocerciasis.
OvB20 is an antigen of Onchocerca volvulus preferentially recognized by sera from cattle vaccinated with irradiated infective larvae of Onchocerca lienalis. Antibodies raised against the recombinant protein were used to characterize the expression of the native protein in different developmental stages of O. volvulus and the rodent filaria Acanthocheilonema viteae. In O. volvulus, antibodies reacted to a polypeptide of 42 kDa in microfilariae and with proteins of 52 and 65 kDa in third-stage larvae. No products were detected in adult stages. Immunogold electron microscopy localized the native protein to discrete patches of the hypodermis and cuticle of infective larvae. Characterization of a homologous protein in A. viteae confirmed the stage-specific expression in infective larvae of the 65-kDa protein, which was secreted during in vitro culture. Vaccination of rodents against A. viteae with a B20-maltose-binding-protein fusion protein resulted in a 49 to 60% reduction in adult worm recoveries with a corresponding 97% reduction in microfilaremia.
Taylor
M J
MJ
Liverpool School of Tropical Medicine, United Kingdom.
Abdel-Wahab
N
N
Wu
Y
Y
Jenkins
R E
RE
Bianco
A E
AE
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology* / Q000378:metabolism; D002417:Cattle; D003001:Cloning, Molecular; D005849:Gerbillinae; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D017181:Onchocerca volvulus / Q000276:immunology* / Q000648:ultrastructure; D009855:Onchocerciasis / Q000517:prevention & control*; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology
D000947:Antigens, Helminth; D015800:Protozoan Proteins; D014614:Vaccines, Synthetic