Melatonin binding sites in interstitial cells from immature rat testes.
Melatonin binding sites in rat testes interstitial cells were identified using 2-[125I]-iodomelatonin. Saturation studies of cells revealed a single class of high affinity binding sites, with an apparent equilibrium dissociation constant (Kd) of 100 +/- 20 pM, and a total binding capacity (B max) of 3.0 +/- 0.2 x 10(3) melatonin molecules per cell. Binding was reversible and inhibited by non radioactive melatonin. These results suggest that interstitial cells from immature rat testes have specific receptors for melatonin.
Vera
H
H
Unidad de Biología de la Reproducción, INTA, Universidad de Chile, Santiago.
Tijmes
M
M
Ronco
A M
AM
Valladares
L E
LE
D000818:Animals; D001665:Binding Sites; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D008550:Melatonin / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012741:Sexual Maturation; D013737:Testis / Q000166:cytology / Q000378:metabolism; D013997:Time Factors
C044556:2-iodomelatonin; D008550:Melatonin