Isolation of SagI, a new HaeIII isoschizomer from Streptococcus agalactiae.
A new HaeIII isoschizomer from Streptococcus agalactiae was isolated by a single-step purification method. The highly active restriction endonuclease, SagI, was free of nonspecific nuclease activity and was suitable for use in molecular biology procedures. The rapid isolation procedure may be applicable for the recovery of other restriction endonucleases from bacteria.
Poch
M T
MT
U. S. Department of Agriculture, Agricultural Research Service, Philadelphia, PA 19118, USA.
Somkuti
G A
GA
D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification* / Q000378:metabolism; D013292:Streptococcus agalactiae / Q000201:enzymology*; D013379:Substrate Specificity
D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases