title: Contribution of the TCR alpha-chain to the differential recognition of bacterial and retroviral superantigens.
abstract: The TCR alpha-chain has been implicated in superantigen recognition by some T cells. In this study, recognition of a retroviral (minor lymphocyte stimulating Ag-1, Mls-1) and a bacterial (staphylococcal enterotoxin B, SEB) superantigen by T cells expressing an identical (transgenic) V beta 8.1 beta-chain and either a V alpha 2+ or V alpha 11+ alpha-chain have been compared. Individual hybridomas were found to vary in their recognition of the two superantigens. Whereas some T cell hybridomas recognized both superantigens comparably, other hybridomas preferentially recognized either Mls-1 or SEB, indicating that the contribution of the TCR alpha-chain to recognition of these superantigens is not equivalent. The degree of heterogeneity in recognition varied between the V alpha 2+ and V alpha 11+ hybridomas, although there were individual hybridomas within each group with distinct patterns of reactivity that varied only in CDR3 alpha. These data are consistent with recent evidence that recognition of Mls-1 and SEB involves distinct TCR/MHC interactions.
authors.affiliation: Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: K
authors.initials: K
authors.lastname: Daly
authors.forename: P
authors.initials: P
authors.lastname: Nguyen
authors.forename: D
authors.initials: D
authors.lastname: Hankley
authors.forename: W J
authors.initials: WJ
authors.lastname: Zhang
authors.forename: D L
authors.initials: DL
authors.lastname: Woodland
authors.forename: M A
authors.initials: MA
authors.lastname: Blackman
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D001483:Base Sequence; D004768:Enterotoxins / Q000276:immunology*; D005434:Flow Cytometry; D006825:Hybridomas / Q000276:immunology; D007376:Interleukin-2 / Q000032:analysis; D051379:Mice; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D016747:Minor Lymphocyte Stimulatory Antigens / Q000276:immunology*; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*; D013210:Staphylococcus / Q000276:immunology*; D018089:Superantigens / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D004768:Enterotoxins; D007376:Interleukin-2; D016747:Minor Lymphocyte Stimulatory Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens; C031818:enterotoxin B, staphylococcal