title: Human platelet antigen gene frequencies in the Austrian population.
abstract: Gene frequencies for the human platelet antigen systems HPA-1, -2, -3, and -5 were determined directly from DNA isolated from cord blood of more than 900 randomly selected Caucasoid newborns in Vienna, Austria. Genotyping was performed by specific amplification of the respective regions coding for platelet glycoproteins GP Ib, IIb, IIIa, and Ia by PCR. These PCR products were analyzed after restriction enzyme digestion and electrophoresis. The observed gene frequencies were: HPA-1a: 0.852, HPA-1b: 0.148; HPA-2a: 0.918, HPA-2b: 0.082; HPA-3a: 0.612, HPA-3b: 0.388; HPA-5a: 0.892, HPA-5b: 0.108. There was a good fit with the Hardy-Weinberg equilibrium. Results from serological determinations and genotyping showed no discrepancies.
authors.affiliation: Clinical Department for Blood Group Serology, University of Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Holensteiner
authors.forename: S
authors.initials: S
authors.lastname: Walchshofer
authors.forename: A
authors.initials: A
authors.lastname: Adler
authors.forename: E M
authors.initials: EM
authors.lastname: Kittl
authors.forename: W R
authors.initials: WR
authors.lastname: Mayr
authors.forename: S
authors.initials: S
authors.lastname: Panzer
mesh_terms: D016824:Antigens, Human Platelet / Q000145:classification / Q000235:genetics*; D001317:Austria; D015331:Cohort Studies; D004247:DNA / Q000032:analysis; D005312:Fetal Blood / Q000276:immunology; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D016133:Polymerase Chain Reaction; D011897:Random Allocation; D044465:White People / Q000235:genetics
chemical_list: D016824:Antigens, Human Platelet; D004247:DNA