title: New cell lines of gastric and pancreatic cancer: distinct morphology, growth characteristics, expression of epithelial and immunoregulatory antigens.
abstract: Two new cell lines from stomach cancers and one from a pancreatic carcinoma are presented. MZ-GC-1 was established from a hepatic metastasis of a well differentiated gastric adenocarcinoma. MZ-GC-2 was derived from ascites induced by a poorly differentiated gastric adenocarcinoma. MZ-PC-1 originated from the pleural effusion of a moderately well differentiated pancreatic ductal adenocarcinoma. MZ-GC-1 cells were adherent and partially polarized, connected tightly via desmosomes. In contrast MZ-GC-2 cells consisted of slightly adherent or floating subpopulations and displayed no desmosomes. MZ-PC-1 cells were adherent and showed polarized growth, connected by apical junctional complexes. Cell doubling times were 7 days for MZ-GC-1 and 45 h for MZ-GC-2 and MZ-PC-1 cells. MZ-GC-2 and MZ-PC-1 gave rise to nude mouse tumours, resembling the original lesions. Chromosome analysis of the cell lines revealed a high range of numerical abnormalities. Each cell line had cytokeratin patterns fitting well to typical in vivo patterns. Furthermore the cell lines expressed a panel of antigens typical for gastrointestinal epithelia. Unique for MZ-PC-1 were high amounts of secreted Ca19-9. gamma-Interferon enhanced HLA-class I antigens up to twofold and induced ICAM-1 expression on each cell line. HLA-class II antigens were differentially enhanced by gamma-interferon. Due to their distinct characteristics the three tumour cell lines may be useful models in the investigation of the cell biology and immunogenicity of gastrointestinal tumours.
authors.affiliation: I. Medizinische Klinik und Poliklinik, Johannes Gutenberg-Universität, Mainz, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Heike
authors.forename: O
authors.initials: O
authors.lastname: Röhrig
authors.forename: H E
authors.initials: HE
authors.lastname: Gabbert
authors.forename: R
authors.initials: R
authors.lastname: Moll
authors.forename: K H
authors.initials: KH
authors.lastname: Meyer zum Büschenfelde
authors.forename: W G
authors.initials: WG
authors.lastname: Dippold
authors.forename: A
authors.initials: A
authors.lastname: Knuth
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000954:Antigens, Surface / Q000032:analysis; D014408:Biomarkers, Tumor / Q000032:analysis; D002454:Cell Differentiation; D002455:Cell Division; D002461:Cell Line, Transformed; D004848:Epithelium / Q000276:immunology / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009362:Neoplasm Metastasis / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000473:pathology*; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D000954:Antigens, Surface; D014408:Biomarkers, Tumor