[The effects of Ebselen upon post-ischemic functional recovery in rat heart].
The effects of Ebselen (DR-3305) upon cardiac function and CK leakage with or without ischemia reperfusion insults were investigated in this isolated working rat heart study. In order to evaluate the effects of Ebselen upon cardiac function, it was administrated to the isolated rat heart with Langendorff perfusion for 15 min at 100 cmH2O. Cardiac functions such as aortic and coronary flow, heart rate and aortic pressure, before and after Ebselen treatment, were measured and expressed as percent of the control value. The coronary resistance was significantly reduced in 100 nM, 500 nM and 1000 nM of Ebselen pretreatment with dose dependent manner. However pretreatment with 1000 nM of Ebselen significantly decreased cardiac output with significant increase of CK leakage. The myocardial protective effects of Ebselen in the cardioplegia or reperfusate were investigated under conditions of normothermic ischemia. After measuring preischemic cardiac functions, the St. Thomas' Hospital cardioplegic solution was infused for 3 min followed by 35 min of global ischemia at 37 degrees C. Hearts were then aerobically reperfused for 15 min in the Langendorff mode. Subsequently, cardiac function was measured and expressed as percent of the preischemic control value. Ebselen addition to the St. Thomas' cardioplegic solution did not possess any myocardial protective effects, although addition to reperfusate possess the protective abilities at a concentration of 10 nM. From these data, Ebselen, which was one of the free radical scavenger, had the myocardial protective effects if it was added to reperfusate at a concentration of 10 nM.
Ishikawa
T
T
Department of Cardiovascular Surgery, National Cardiovascular Center, Osaka, Japan.
Yamamoto
F
F
Ohashi
T
T
Shimada
Y
Y
Kagisaki
K
K
Kumada
Y
Y
Kito
Y
Y
Kawashima
Y
Y
D000818:Animals; D001393:Azoles / Q000494:pharmacology*; D003326:Coronary Circulation; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D054833:Isoindoles; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism; D016566:Organoselenium Compounds / Q000494:pharmacology*; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar
D001393:Azoles; D054833:Isoindoles; D016566:Organoselenium Compounds; C042986:ebselen