title: The essential helicase gene RAD3 suppresses short-sequence recombination in Saccharomyces cerevisiae.
abstract: We have isolated an allele of the essential DNA repair and transcription gene RAD3 that relaxes the restriction against recombination between short DNA sequences in Saccharomyces cerevisiae. Double-strand break repair and gene replacement events requiring recombination between short identical or mismatched sequences were stimulated in the rad3-G595R mutant cells. We also observed an increase in the physical stability of double-strand breaks in the rad3-G595R mutant cells. These results suggest that the RAD3 gene suppresses recombination involving short homologous sequences by promoting the degradation of the ends of broken DNA molecules.
authors.affiliation: Department of Molecular Genetics, Beckman Research Institute, City of Hope National Medical Center, Duarte, California 91010, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Bailis
authors.forename: S
authors.initials: S
authors.lastname: Maines
authors.forename: M T
authors.initials: MT
authors.lastname: Negritto
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics*; D000483:Alleles; D001483:Base Sequence; D015139:Blotting, Southern; D004249:DNA Damage; D004265:DNA Helicases / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics; D004271:DNA, Fungal / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics*; D005804:Genes, Lethal / Q000235:genetics; D016681:Genome, Fungal; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics; D011995:Recombination, Genetic; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D013489:Suppression, Genetic
chemical_list: D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D000251:Adenosine Triphosphatases; C061897:Rad3 protein, S cerevisiae; D004265:DNA Helicases