Two cDNA clones encoding 14-3-3 homologs from tomato fruit.
Two tomato cDNA clones with homology to the 14-3-3 family of proteins were identified through immunoscreening a ripening tomato fruit library (Clontech). These two clones share approx. 71% identity at the nucleotide level and 84% identity at the deduced amino acid level, with radical amino acid substitutions clustering at the acidic carboxy-terminus. Southern hybridization data indicate that each clone represents a unique gene. Distinct transcript accumulation patterns during tomato fruit ripening together with the homology to brain regulatory proteins suggest potential involvement in fruit development.
Laughner
B
B
Department of Horticultural Sciences, University of Florida, Gainesville 32611, USA.
Lawrence
S D
SD
Ferl
R J
RJ
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000737:chemistry; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D018076:DNA, Complementary; D011506:Proteins; D014446:Tyrosine 3-Monooxygenase