Sequence of Asr2, a member of a gene family from Lycopersicon esculentum encoding chromosomal proteins: homology to an intron of the polygalacturonase gene.
Asr is a family of genes regulated by abscisic acid, stress and ripening in tomato. Asr2, a recently reported member of this family, has been further characterized through sequencing of a genomic clone. We report the sequencing of 2029 bp of Asr2, spanning its AT-rich (62%) upstream region with probable regulatory functions. This region displays several candidate TATA and CAAT boxes that might be involved in transcription initiation, as well as a motif similar to one previously reported to be responsible for induction by ABA. Apart from that, we found a 108-bp stretch from the 3' non-coding region which displays a high homology (92%) to a region within intron 6 of the polygalacturonase gene, which, like Asr2, is expressed in ripening tomato fruit. This striking similarity suggests the presence of either a conserved regulatory motif or an abundant mobile element.
Rossi
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Departamento de Ciencias Biológicas-Area Genética Molecular y Biotecnología-, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Argentina.
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D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D017343:Genes, Plant; D007438:Introns / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010940:Plant Proteins / Q000235:genetics*; D011096:Polygalacturonase / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
C091854:Asr2 protein, Lycopersicon esculentum; D018744:DNA, Plant; D010940:Plant Proteins; D011096:Polygalacturonase