Oestrogen and progesterone receptors in normal human endometrium: comparison of immunocytochemical analysis on frozen and paraffin sections with or without enzymatic pretreatment.
Immunocytochemical study in paraffin sections of human endometrium showed that the receptor contents for both oestrogen and progesterone receptors were lower than in the frozen sections although the staining patterns were similar in these two section types. Pretreating the specimens with proteolytic enzymes like trypsin, DNase and pronase improved the oestrogen receptor staining but a better result with progesterone receptor staining was obtained when no enzymatic pretreatment was applied to the sections.
Fung
H Y
HY
Department of Obstetrics and Gynaecology, Prince of Wales Hospital, Chinese University of Hong Kong, Shatin.
Wong
Y L
YL
Wong
F W
FW
Rogers
M S
MS
D003851:Deoxyribonucleases / Q000494:pharmacology; D004717:Endometrium / Q000737:chemistry*; D010450:Endopeptidases / Q000494:pharmacology*; D005260:Female; D005629:Frozen Sections; D006801:Humans; D007150:Immunohistochemistry; D010232:Paraffin; D011402:Pronase / Q000494:pharmacology; D011960:Receptors, Estrogen / Q000032:analysis*; D011980:Receptors, Progesterone / Q000032:analysis*; D014357:Trypsin / Q000494:pharmacology
D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D010232:Paraffin; D003851:Deoxyribonucleases; D010450:Endopeptidases; D014357:Trypsin; D011402:Pronase