Palmitoylation of endogenous and viral acceptor proteins by fatty acyltransferase (PAT) present in erythrocyte ghosts and in placental membranes.
Human erythrocyte ghosts were shown to have palmitoylating activity which acylates both endogenous ghost polypeptides and exogenous proteins derived from Semliki Forest virus (SFV). Cell-free fatty acid transfer from [3H]palmitoyl-CoA to endogenous protein was greatly enhanced in ghosts when pre-existing fatty acids linked to the endogenous acyl proteins were removed by hydroxylamine treatment prior to the transfer reaction. In contrast to erythrocyte acyl proteins acceptor proteins present in human placental membranes were palmitoylated in vitro to a similar extent with or without prior deacylation by hydroxylamine treatment. This indicates the presence of large pools of non-acylated proteins in placenta and small pools in erythrocytes. In testing for the protein substrate specificity of the palmitoyl transferase (PAT) present in ghosts we found that the SFV acceptor proteins, which are totally unrelated to erythrocytes, competed with the palmitoylation of endogenous ghost protein acceptors. This palmitoylating enzyme is inhibited by Cibacron Blue, SDS, and heat treatment, but stimulated in the presence of low concentrations of mild detergent (TX-100). Since PAT operating at the surface membrane of red blood cells has properties very similar to those of PAT present in human placental microsomes [1], we suggest that only one type of PAT may transfer fatty acids to various acylproteins that occur at multiple locations in different tissues [2].
Schmidt
M F
MF
Institut für Immunologie und Molekularbiologie, Fachbereich Veterinärmedizin, Freie Universität Berlin, Germany.
McIlhinney
R A
RA
Burns
G R
GR
D000215:Acylation; D000217:Acyltransferases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000201:enzymology*; D005453:Fluorescence; D006801:Humans; D019811:Hydroxylamine; D006898:Hydroxylamines / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D017830:Octoxynol / Q000494:pharmacology; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D010920:Placenta / Q000201:enzymology*; D012672:Semliki forest virus / Q000737:chemistry; D013696:Temperature; D014227:Triazines / Q000494:pharmacology; D014764:Viral Proteins / Q000378:metabolism*
D006898:Hydroxylamines; D014227:Triazines; D014764:Viral Proteins; D010171:Palmitoyl Coenzyme A; D019811:Hydroxylamine; C012842:Cibacron Blue F 3GA; D017830:Octoxynol; D000217:Acyltransferases; C043421:glycoprotein N-palmitoyltransferase