Identification of two highly homologous presynaptic proteins distinctly localized at the dendritic and somatic synapses.
Through screening of a murine brain cDNA library, we have isolated two brain specific cDNAs encoding highly homologous proteins, named 921-L and 921-S, comprised of 134 amino acids with 80% identity. Immunohistological study with the mAbs raised against the bacterially expressed 921 proteins showed that 921-L protein is distributed at the dendritic region and 921-S at the neuronal somatic surface. Immuno-electron microscopic study revealed that both 921 proteins are localized at the presynaptic terminal, indicating that the 921 proteins are differentially expressed at the dendritic and somatic presynapses.
Takahashi
S
S
Department of Immunology, Kochi Medical School, Japan.
Yamamoto
H
H
Matsuda
Z
Z
Ogawa
M
M
Yagyu
K
K
Taniguchi
T
T
Miyata
T
T
Kaba
H
H
Higuchi
T
T
Okutani
F
F
D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000166:cytology / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D003712:Dendrites / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013569:Synapses / Q000378:metabolism*
D033942:Adaptor Proteins, Vesicular Transport; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; C095992:complexin I; C095993:complexin II