title: Subcellular and intranuclear localization of neptunium-237 (V) in rat liver.
abstract: The present investigation was aimed at establishing the distribution of 237Np within the different structures of hepatocytes. Rats were contaminated experimentally by intravenous injection of 237Np (V) and the subcellular structures of the liver were separated by ultracentrifugation. Twenty-four hours after contamination, the nuclear and cytosolic fractions bound 54 and 32%, respectively, of the total radionuclide. Purification of the nuclei followed by dissociation of the protein components in medium of increasing ionic strength showed a specific binding of neptunium to the structural proteins of the nuclear matrix.
authors.affiliation: Laboratoire de Radiotoxicologie, CEA. DSV. DPTE, Bruyères le châtel, France.
authors.forename: F
authors.initials: F
authors.lastname: Paquet
authors.forename: M
authors.initials: M
authors.lastname: Verry
authors.forename: G
authors.initials: G
authors.lastname: Grillon
authors.forename: C
authors.initials: C
authors.lastname: Landesman
authors.forename: R
authors.initials: R
authors.lastname: Masse
authors.forename: D M
authors.initials: DM
authors.lastname: Taylor
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D009405:Neptunium / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D009405:Neptunium