title: Strategies for the synthesis and screening of glycoconjugates. 2. Covalent immobilization for flow cytometry.
abstract: Glycosylamines are readily available carbohydrate derivatives that undergo acylation reactions with homobifunctional N-hydroxysuccinimidyl esters. The product glycosylamides carry a spacer group equipped with one active ester functionality. This route provides well-defined glycoconjugates, which may be cross-linked to various amino-functionalized resins. Carbohydrate recognition of the resulting sugar-bead conjugates is probed by lectin immunostaining or flow cytometry using a fluorescently labeled lectin.
authors.affiliation: Affymax Research Institute, Palo Alto, California 94304, USA.
authors.forename: D
authors.initials: D
authors.lastname: Vetter
authors.forename: E M
authors.initials: EM
authors.lastname: Tate
authors.forename: M A
authors.initials: MA
authors.lastname: Gallop
mesh_terms: D002240:Carbohydrate Sequence; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006001:Glycoconjugates / Q000032:analysis / Q000138:chemical synthesis*; D037102:Lectins / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D005456:Fluorescent Dyes; D006001:Glycoconjugates; D037102:Lectins