Protective effect of amphotericin B against lethal photodynamic treatment in yeast.
The effect of polyenic antibiotic amphotericin B on photodynamically induced cell damage was investigated using Kluyveromyces fragilis. The photosensitizers applied are known to act via cell membrane damage (rose bengal and toluidine blue) or via DNA modification causing genotoxic effects (8-methoxypsoralen). Methylene blue was shown to cause membrane damage comparable with the effect of rose bengal and toluidine blue. Under conditions of photodynamic damage a pronounced protective effect of the antibiotic was evident in increased cell survival with all of the photosensitizers tested. Mitochondrial activity indicated a tendency of the antibiotic to protect the cells. The protective role of amphotericin B is discussed in the light of possible implications for photodynamic therapy of microbial infections.
Lazarova
G
G
Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bontchev, Sofia.
Tashiro
H
H
D000666:Amphotericin B / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects*; D004249:DNA Damage; D007716:Kluyveromyces / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects*; D008027:Light; D008730:Methoxsalen / Q000494:pharmacology; D008751:Methylene Blue / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism; D017319:Photosensitizing Agents / Q000494:pharmacology*; D012395:Rose Bengal / Q000494:pharmacology; D014048:Tolonium Chloride / Q000494:pharmacology
D017319:Photosensitizing Agents; D014048:Tolonium Chloride; D012395:Rose Bengal; D000666:Amphotericin B; D008751:Methylene Blue; D008730:Methoxsalen