Combined FISH painting and harlequin staining for cell cycle-controlled chromosome analysis in human lymphocytes.
We present a simple method that allows scoring of FISH-painted chromosomes exclusively in the first division human lymphocytes. It consists of a combination of FISH with chromosome-specific libraries and a differential sister chromatid staining after BrdU treatment of lymphocyte cultures. The method allows a precise quantification of induced chromosome damage for human biodosimetry.
Kulka
U
U
Institut für Strahlenbiologie, GSF-Forschungszentrum für Umwelt und Gesundheit Neuherberg, Oberschleissheim, Germany.
Huber
R
R
Müller
P
P
Knehr
S
S
Bauchinger
M
M
D002453:Cell Cycle; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000648:ultrastructure*