title: Extracellular adenosine triphosphate. A shock to hemopoietic cells.
abstract: Many or perhaps all cell types in the body possess extracellular binding sites for nucleosides and nucleotides, the purinoceptors. The binding sites that favor adenosine triphosphate (ATP) have been classified as P2-purinoceptors. One subclass of the P2-purinoceptors is the P2z-purinoceptor that mediates the permeabilizing effect of ATP4- (the fully ionized form of adenosine triphosphate). In the presence of millimolar concentrations of ATP4-, this receptor, which was found on all cells of hemopoietic origin but not on cells of stromal origin, renders the sensitive cells permeable for molecules up to 1 kD. This property has been used to eliminate cells of hemopoietic origin from mixed populations. Skeletal- and blood-forming tissues have a complex cellular composition of predominantly stromal and hemopoietic cells. The 2 cell types influence each others' formation, differentiation, and activities in a largely unclarified manner. Rigorous separation would help the study of the properties and potencies of the 2 cell types and their mutual interactions. A short treatment of cell populations isolated from bone, bone marrow, or cartilage with 2 mM adenosine triphosphate and 1 mM of cytotoxic, but not permeant, potassium thiocyanate obliterates all cells of hemopoietic origin, including macrophages, osteoclasts, and their progenitors from these populations.
authors.affiliation: Department of Cell Biology, Faculty of Medicine, Leiden University, The Netherlands.
authors.forename: P J
authors.initials: PJ
authors.lastname: Nijweide
authors.forename: W E
authors.initials: WE
authors.lastname: Modderman
authors.forename: C E
authors.initials: CE
authors.lastname: Hagenaars
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001854:Bone Marrow Cells; D001842:Bone and Bones / Q000166:cytology; D002356:Cartilage / Q000166:cytology; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002469:Cell Separation / Q000379:methods; D004396:Coloring Agents; D004996:Ethidium; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D018048:Receptors, Purinergic P2 / Q000187:drug effects* / Q000502:physiology; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects*; D013861:Thiocyanates / Q000494:pharmacology
chemical_list: D004396:Coloring Agents; D018048:Receptors, Purinergic P2; D013861:Thiocyanates; D000255:Adenosine Triphosphate; D004996:Ethidium; C009941:potassium thiocyanate