title: Replication of human herpesvirus type 6 (strain AJ) in JJHan cells grown in protein-free medium.
abstract: The human T-cell line JJHan, commonly used as a substrate for human herpesvirus type 6 (HHV-6) replication, was established in a protein-free 1:1 mixture of Ham's F12 and Iscove's modified Dulbecco's medium. After 250 passages (4 years) in protein-free medium, the cells, designated JJHan-PF, were cocultured with JJHan cells chronically infected with strain AJ of HHV-6 conventionally grown in serum-supplemented medium. The production of viral antigen was monitored by indirect immunofluorescent antibody staining. The infectivity of the virus in JJHan-PF cells was similar to that in JJHan cultures as shown by HHV-6 antigen detection using a convalescent phase serum. The results show that JJHan-PF cells provide a sensitive substrate for HHV-6 replication without the interference of serum or other proteins usually added to the culture medium.
authors.affiliation: Centre of Hygiene, J.W. Goethe University, Frankfurt a. M. Germany.
authors.forename: J
authors.initials: J
authors.lastname: Cinatl
authors.forename: J
authors.initials: J
authors.lastname: Cinatl
authors.forename: B
authors.initials: B
authors.lastname: Weber
authors.forename: H
authors.initials: H
authors.lastname: Rabenau
authors.forename: H O
authors.initials: HO
authors.lastname: Gümbel
authors.forename: B
authors.initials: B
authors.lastname: Kornhuber
authors.forename: H W
authors.initials: HW
authors.lastname: Doerr
mesh_terms: D002460:Cell Line / Q000821:virology*; D003470:Culture Media; D015654:Herpesvirus 6, Human / Q000502:physiology*; D006801:Humans; D013601:T-Lymphocytes / Q000821:virology; D014779:Virus Replication
chemical_list: D003470:Culture Media