title: Construction of an Actinobacillus pleuropneumoniae-Escherichia coli cosmid cloning vector [Gene 160 (1995) 81-86].
abstract: We constructed a cosmid vector, pSW206, which should be useful for the construction of genomic libraries of Actinobacillus pleuropneumoniae (Apl) DNA. pSW206 is based on the broad-host-range plasmid RK2 and can be introduced into Apl by conjugation.
authors.affiliation: Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: West
authors.forename: M J
authors.initials: MJ
authors.lastname: Romero
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D003227:Conjugation, Genetic; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D015698:Genomic Library; D010641:Phenotype; D010957:Plasmids; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial