Time-dependent effect of glutaraldehyde on the tendency to calcify of both autografts and xenografts.
To determine mechanisms responsible for the reduced calcification in short-term glutaraldehyde (Glu)-treated autologous pericardial bioprostheses, we studied the time effect of Glu on subsequent calcification and differences in calcification of autograft and xenograft implants in a rat subcutaneous implantation model. In experiment 1, four groups of bovine pericardial pieces (1 cm2) were prepared: (A) fresh bovine pericardium without Glu, (B) with 15-minute Glu, (C) with 60-minute Glu, and (D) with 120-minute Glu. Seven young male Sprague-Dawley rats were used; each received four bovine pericardial pieces from group A, B, C, or D for subcutaneous implantation. Calcium content of the implants (microgram/mg dry weight) 45 days later was 4.8 +/- 2.9, 29.8 +/- 13.6, 106.3 +/- 13.7, and 176.3 +/- 85.5 in groups A, B, C, and D, respectively (p < 0.05 between any two groups). Experiment 2 used 8 young male Sprague-Dawley rats from different mothers. Each received five subcutaneous skin implants. The five skin implants were prepared as follows: (1) fresh self skin, (2) self skin with 30-minute Glu, (3) self skin with 48-hour Glu, (4) fresh skin of others, and (5) skin of others with 48-hour Glu. After 45 days of implantation, the calcium content of the implants was 1.4 +/- 1.1, 57.9 +/- 35.4, 142.7 +/- 61.4, 1.5 +/- 1.1, and 94.9 +/- 24.1 micrograms/mg dry weight in groups 1, 2, 3, 4, and 5, respectively (p < 0.05 for 1 versus 2, 3, or 5; 2 versus 3, 4, or 5; 3 versus 4; and 4 versus 5).(ABSTRACT TRUNCATED AT 250 WORDS)
Liao
K
K
Department of Cardiothoracic Surgery, Montefiore Medical Center, Albert Einstein College of Medicine, New York, New York, USA.
Frater
R W
RW
LaPietra
A
A
Ciuffo
G
G
Ilardi
C F
CF
Seifter
E
E
D000818:Animals; D001705:Bioprosthesis; D002114:Calcinosis / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D002118:Calcium / Q000032:analysis; D005976:Glutaral / Q000494:pharmacology*; D006350:Heart Valve Prosthesis; D006351:Heart Valves / Q000737:chemistry / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000737:chemistry; D016038:Skin Transplantation; D013997:Time Factors; D014182:Transplantation, Autologous; D014183:Transplantation, Heterologous
D002118:Calcium; D005976:Glutaral