Purification and immunochemistry of a soluble matrix protein of the chicken eggshell (ovocleidin 17).
The protein components of biomineralized structures (matrix proteins) are believed to modulate crystal nucleation and growth, and thereby influence the shape and strength of the final structure. The chicken eggshell contains a complex array of distinct matrix proteins. The most abundant of these was purified to homogeneity by a combination of anionic exchange and hydroxyapatite chromatographies. Antibodies to this protein were raised in rabbit, and utilized for Western blotting and immunohistochemistry. These studies indicated that the 17 kDa antigen (ovocleidin 17, OC-17) is found in the shell gland mucosa, and that only the tubular gland cells were positive. Immunohistochemistry with decalcified shell indicated that OC-17 is uniformly distributed throughout the shell matrix, but concentrated in the mammillary bodies. Our results indicate that this protein is secreted during shell formation and becomes incorporated into this structure. It may therefore play a role in the crystallization process and influence the properties of the resulting eggshell.
Hincke
M T
MT
Department of Anatomy and Neurobiology, University of Ottawa, Canada.
Tsang
C P
CP
Courtney
M
M
Hill
V
V
Narbaitz
R
R
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D015153:Blotting, Western; D002645:Chickens; D002852:Chromatography, Ion Exchange; D004527:Egg Proteins / Q000032:analysis / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification*; D004528:Egg Shell / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D007120:Immunochemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight
D000596:Amino Acids; D004527:Egg Proteins; C095273:ovocleidin 17