Non-destructive analysis of the protein conformational structure of human pituitary adenomas using reflectance FT-IR microspectroscopy.
Reflectance Fourier-transform infrared microspectroscopy was used to determine the secondary structure and composition of the different human pituitary adenomas. Non-functioning pituitary adenomas exhibited similar protein secondary structure and conformational composition, but active pituitary adenomas revealed different behavior. The differences in secondary structure for the different human pituitary adenomas might possibly be due to the different protein conformations of the proliferated adenoma tissues and various hormones shared.
Lee
L S
LS
Neurological Institute, Veterans General Hospital-Taipei, Taiwan, Republic of China.
Lin
S Y
SY
Chi
C W
CW
Liu
H C
HC
Cheng
C L
CL
D000236:Adenoma / Q000737:chemistry*; D000328:Adult; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000737:chemistry*; D010907:Pituitary Hormones / Q000737:chemistry*; D010911:Pituitary Neoplasms / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D009363:Neoplasm Proteins; D010907:Pituitary Hormones