title: Identification of a new member of the human eIF-5A gene family.
abstract: Using an oligodeoxynucleotide generated by rapid PCR amplification of 5'-cDNA ends (5'-RACE) as a detection probe, we have isolated a new genomic clone encoding the human eukaryotic initiation factor 5A (eIF-5A). Sequence analysis revealed that the eIF-5A coding region is identical to the corresponding cDNA but interrupted by three introns. In a plasmid shuffle experiment we show functional replacement of the essential homologous gene in Saccharomyces cerevisiae by this human eIF-5A.
authors.affiliation: SANDOZ Research Institute, Vienna, Austria.
authors.forename: K
authors.initials: K
authors.lastname: Koettnitz
authors.forename: T
authors.initials: T
authors.lastname: Wöhl
authors.forename: B
authors.initials: B
authors.lastname: Kappel
authors.forename: F
authors.initials: F
authors.lastname: Lottspeich
authors.forename: J
authors.initials: J
authors.lastname: Hauber
authors.forename: D
authors.initials: D
authors.lastname: Bevec
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005816:Genetic Complementation Test; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D010448:Peptide Initiation Factors / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016601:RNA-Binding Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D000097574:Eukaryotic Translation Initiation Factor 5A
chemical_list: D018076:DNA, Complementary; D010448:Peptide Initiation Factors; D016601:RNA-Binding Proteins; D000097574:Eukaryotic Translation Initiation Factor 5A