title: Identification of rat urinary kinin as bradykinin.
abstract: Bradykinin (BK)-like activity, which was detected by BK-enzyme-immunoassay, was purified from 80 ml of ureter urine of Sprague-Dawley rats by Sephadex G 25 chromatography, FPLC, and reversed phase HPLC. The purified kinin fraction showed the same retention time as authentic BK on HPLC and produced contraction of isolated rat uterus, the contraction being suppressed by a B2-antagonist Hoe140. There was no other kinin detected on the HPLC at the corresponding retention time to kallidin, arginyl-BK or T-kinin. The peptide showed an amino acid sequence identical to that of BK by amino acid sequence analysis.
authors.affiliation: Department of Pharmacology, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hagiwara
authors.forename: M
authors.initials: M
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Kuraishi
authors.forename: I
authors.initials: I
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Miyata
authors.forename: S
authors.initials: S
authors.lastname: Oh-ishi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001681:Biological Assay; D001920:Bradykinin / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017207:Rats, Sprague-Dawley; D017421:Sequence Analysis; D014590:Uterine Contraction / Q000187:drug effects
chemical_list: C065679:icatibant; D001920:Bradykinin