title: [Microbiological assay method for T-3761 concentration in body fluids].
abstract: A microbiological assay method for measurement of T-3761 and its stability in body fluids were investigated. The paper disc method proved suitable for this assay using Escherichia coli Kp as a test organism and commercially available heart infusion agar as a test medium. When using the paper disc method, lower detection limit of T-3761 was approximately 0.05 microgram/ml and 0.1 microgram/ml for 1/15M phosphate buffer (pH 7.0) and human serum, respectively. T-3761 in human serum and urine was stable under freezing -20 degrees C for at least 28 days.
authors.affiliation: Research Laboratories, Toyama Chemical Co., Ltd.
authors.forename: J
authors.initials: J
authors.lastname: Maehana
authors.forename: R
authors.initials: R
authors.lastname: Kitayama
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: K
authors.initials: K
authors.lastname: Kumano
authors.forename: S
authors.initials: S
authors.lastname: Minami
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
authors.forename: Y
authors.initials: Y
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Narita
mesh_terms: D000362:Agar; D000890:Anti-Infective Agents; D001681:Biological Assay / Q000379:methods; D001826:Body Fluids / Q000378:metabolism*; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004355:Drug Stability; D004926:Escherichia coli / Q000187:drug effects; D024841:Fluoroquinolones; D006801:Humans; D010078:Oxazines / Q000493:pharmacokinetics* / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D003470:Culture Media; D024841:Fluoroquinolones; D010078:Oxazines; C078052:pazufloxacin; D000362:Agar