Molecular cloning of the human gene SUVCC2 associated with mutagenesis following the induction of non-dimer DNA damages by solar UV radiation.
A mutant cell line, DRP 512, sensitive to the induction of non-dimer DNA damages produced by solar UV radiation was derived from ICR 2A frog cells. In addition, the DRP 512 cells exhibited an abnormally high level of ouabain-resistant mutants after exposure to solar UV. A level of 1.1. mutants per 10(6) survivors per kJ m-2 was measured for ICR 2A whereas the yield was 4.2 mutants per 10(6) survivors per kJ m-2 for the solar-UV-sensitive cell line. The DRP 512 cells were transfected with human DNA and a secondary transformant obtained in which normal solar UV sensitivity and mutation induction were restored. DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the human gene capable of restoring normal solar UV sensitivity and mutation induction to DRP 512. This gene has been designated SUVCC2.
Rosenstein
B S
BS
Department of Radiation Oncology, Mount Sinai School of Medicine, City University of New York, NY 10029, USA.
Vaslet
C A
CA
D000328:Adult; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D003001:Cloning, Molecular; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005347:Fibroblasts / Q000528:radiation effects; D005796:Genes / Q000528:radiation effects*; D015698:Genomic Library; D006801:Humans; D016296:Mutagenesis; D010782:Photolysis; D011898:Ranidae; D012867:Skin / Q000528:radiation effects*; D013472:Sunlight; D014466:Ultraviolet Rays; D014965:X-Rays