Molecular cloning of cDNA for sapecin B, an antibacterial protein of Sarcophaga, and its detection in larval brain.
A cDNA clone for sapecin B, an antibacterial protein of Sarcophaga, was isolated. This cDNA encoded a precursor protein of sapecin B consisting of a signal sequence (24 residues), prosegment (30 residues) and mature sapecin B (34 residues). Sapecin B was synthesized almost exclusively in the fat body when the larval body wall was injured, but the brain of naive larvae was also demonstrated to contain a significant amount of sapecin B. These findings suggested that sapecin B is a bifunctional protein.
Lee
S R
SR
Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Kurata
S
S
Natori
S
S
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents; D001483:Base Sequence; D001921:Brain / Q000196:embryology; D001923:Brain Chemistry; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004175:Diptera / Q000196:embryology / Q000235:genetics*; D007301:Insect Hormones / Q000235:genetics*; D019476:Insect Proteins; D007814:Larva / Q000737:chemistry; D008969:Molecular Sequence Data
D000890:Anti-Infective Agents; D018076:DNA, Complementary; D007301:Insect Hormones; D019476:Insect Proteins; C080667:sapecin B protein, Sarcophaga peregrina