title: Cloning and characterization of a human type II receptor for bone morphogenetic proteins.
abstract: Bone morphogenetic proteins (BMPs) are members of the transforming growth factor beta superfamily. Several members of this family have been shown to transduce their signals through binding to type I and type II serine-(threonine) kinase receptors. Here we report the cDNA cloning and characterization of a human type II receptor for BMPs (BMPR-II), which is distantly related to DAF-4, a BMP type II receptor from Caenorhabditis elegans. In transfected COS-1 cells, osteogenic protein (OP)-1/BMP-7, and less efficiently BMP-4, bound to BMPR-II. BMPR-II bound ligands only weakly alone, but the binding was facilitated by the presence of previously identified type I receptors for BMPs. Binding of OP-1/BMP-7 to BMPR-II was also observed in nontransfected cell lines. Moreover, a transcriptional activation signal was transduced by BMPR-II in the presence of type I receptors after stimulation by OP-1/BMP-7.
authors.affiliation: Synergen, Inc., Boulder, CO 80301-2546, USA.
authors.forename: B L
authors.initials: BL
authors.lastname: Rosenzweig
authors.forename: T
authors.initials: T
authors.lastname: Imamura
authors.forename: T
authors.initials: T
authors.lastname: Okadome
authors.forename: G N
authors.initials: GN
authors.lastname: Cox
authors.forename: H
authors.initials: H
authors.lastname: Yamashita
authors.forename: P
authors.initials: P
authors.lastname: ten Dijke
authors.forename: C H
authors.initials: CH
authors.lastname: Heldin
authors.forename: K
authors.initials: K
authors.lastname: Miyazono
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D052004:Bone Morphogenetic Protein Receptors; D019485:Bone Morphogenetic Proteins; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D018076:DNA, Complementary; D005909:Glioblastoma; D006133:Growth Substances / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D017978:Receptors, Growth Factor; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013378:Substantia Nigra / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D019485:Bone Morphogenetic Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D006133:Growth Substances; D011506:Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017978:Receptors, Growth Factor; D011994:Recombinant Proteins; D052004:Bone Morphogenetic Protein Receptors