title: [Behavior of Orf virus in permissive and nonpermissive systems].
abstract: Dogs were immunized i.m. with attenuated poxvirus vaccines (vaccinia virus, Orf-virus) and a bovine herpesvirus-1 (BHV-1) vaccine. After intradermal (i.d.) application of the vaccine viruses a specific delayed type hypersensitivity (DTH) reaction of the skin occurred only with vaccinia virus. The i.d. application of Orf-virus caused a short-term, non-specific inflammatory reaction of the skin, even in dogs not immunized with Orf-virus. Out of 30 sera from Orf-virus immunized beagles (n = 4) only eight were found reactive to Orf-virus in a competition ELISA. Three sera from dogs not Orf-virus immunized but skin-tested with the virus contained low antibody titers. Using indirect immunofluorescence (IIF) in flow cytometry, the existence of Orf-virus antigens was examined on the surface and in the cytoplasm of permissive (BFK and Vero)- and questionable permissive MDCK cells. The canine kidney MDCK cell line was found to be non-permissive for Orf-virus replication; the occurrence of an Orf-(ecthyma contagiosum) like disease in dogs is unlikely.
authors.affiliation: Institut für Medizinische Mikrobiologie, Infektions- und Seuchenmedizin, Veterinärmedizinische Fakultät, Universität München.
authors.forename: M
authors.initials: M
authors.lastname: Büttner
authors.forename: C P
authors.initials: CP
authors.lastname: Czerny
authors.forename: M
authors.initials: M
authors.lastname: Schumm
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002522:Chlorocebus aethiops; D004285:Dogs; D004474:Ecthyma, Contagious / Q000276:immunology / Q000517:prevention & control; D004797:Enzyme-Linked Immunosorbent Assay; D007242:Herpesvirus 1, Bovine / Q000276:immunology; D007668:Kidney; D009923:Orf virus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D014613:Vaccines, Attenuated; D014709:Vero Cells; D014765:Viral Vaccines; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines
vernacular_title: Verhalten von Orf-Virus in permissiven und nichtpermissiven Systemen.