Effect of heat stress on follicular development during the estrous cycle in lactating dairy cattle.
In this study we examined, in two experiments, patterns of follicular development and dominance under conditions of heat stress. Estrous cycles were programmed to include two follicular waves (wave 1 and 2). On Day 1 of the estrous cycle (Day 0 = estrus), cows were assigned randomly to cooled (C; n = 6) or heat-stressed (H; n = 6) groups. In experiment 1, on Day 12 prostaglandin (PG) F2 alpha was injected and a controlled intravaginal drug release device (1.9 g progesterone) was inserted (this was removed on Day 17). In experiment 2, PGF 2 alpha was injected on Day 14. Ovarian structures were examined daily by ultrasonography, and blood samples were collected at each scanning. Cycle lengths were 20 and 17 days in experiments 1 and 2, respectively. Mean maximal body temperatures were higher (p < 0.01) in H (40.3 degrees C) than in C (38.8 degrees C) cows. In experiment 1, the rate of increase in number of large follicles (> or = 10 mm) was greater in H than in C cows (p < 0.01), resulting in 53% more large follicles in H cows during wave 1; this was associated with a lower (p < 0.05) number of medium-sized (6-9 mm) follicles between Days 7 and 10 of the cycle. Heat stress hastened (p < 0.02) the decrease in size of the first-wave dominant follicle and hastened (p < 0.01) the emergence of the second dominant (preovulatory) follicle by 2 days.(ABSTRACT TRUNCATED AT 250 WORDS)
Wolfenson
D
D
Department of Animal Science, Faculty of Agriculture, Hebrew University, Rehovot, Israel.
Thatcher
W W
WW
Badinga
L
L
Savio
J D
JD
Meidan
R
R
Lew
B J
BJ
Braw-Tal
R
R
Berman
A
A
D000818:Animals; D001831:Body Temperature; D002417:Cattle / Q000502:physiology*; D004958:Estradiol / Q000097:blood; D004971:Estrus / Q000502:physiology*; D005260:Female; D006358:Hot Temperature; D007774:Lactation / Q000502:physiology*; D006080:Ovarian Follicle / Q000254:growth & development*; D011374:Progesterone / Q000097:blood; D013312:Stress, Physiological / Q000503:physiopathology*
D011374:Progesterone; D004958:Estradiol