High molecular weight polypeptides related to dynein heavy chains in Nicotiana tabacum pollen tubes.
Nicotiana tabacum pollen tubes contain two high molecular weight polypeptides (about 400 kDa), which are specifically expressed during pollen germination and pollen tube growth in BK medium. The high molecular weight doublet resembles the dynein heavy chains in some biochemical properties. Sedimentation profiles of pollen tube extracts show that the high molecular weight bands have sedimentation coefficients of 22 S and 12 S, respectively. ATPase assay of sedimentation fractions shows an activity ten times higher when stimulated by the presence of bovine brain microtubules in fractions containing the 22 S high molecular weight polypeptide. Both these high molecular weight polypeptides can bind microtubules in an ATP-dependent fashion. A mouse antiserum to a synthetic peptide reproducing the sequence of the most conserved ATP-binding site among dynein heavy chains recognized the two high molecular weight polypeptides. Therefore these polypeptides have sequences immunologically related to the ATP binding sites of dynein heavy chains.
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Dipartimento di Biologia Ambientale, Università di Siena, Italia.
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D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D001665:Binding Sites; D001921:Brain / Q000378:metabolism; D002417:Cattle; D004398:Dyneins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D007120:Immunochemistry; D066298:In Vitro Techniques; D008870:Microtubules / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010947:Plants, Toxic; D011058:Pollen / Q000737:chemistry; D014026:Nicotiana / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D000906:Antibodies; D010455:Peptides; D010940:Plant Proteins; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D004398:Dyneins