Characterization of the testis-specific gene 'calmegin' promoter sequence and its activity defined by transgenic mouse experiments.
We have cloned the genomic DNA of calmegin [(1992) J. Biol. Chem. 269, 7744-7749] and analyzed its promoter region. It contained GC-rich sequences and potential binding sites for AP 2 and Sp 1, but lacked the TATA sequence. The 330 bp 5' flanking sequence of calmegin genomic DNA fused with the CAT gene was used for the study of promoter activity in transgenic mice. The CAT gene activity was detected exclusively in testes, indicating that the 330 bp calmegin 5' sequence was sufficient for the testis-specific expression. The existence of testicular nuclear factors specifically bound to the putative promoter sequence was also demonstrated.
Watanabe
D
D
Research Institute for Microbial Diseases, Osaka University, Japan.
Okabe
M
M
Hamajima
N
N
Morita
T
T
Nishina
Y
Y
Nishimune
Y
Y
D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D037281:Calnexin; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013737:Testis / Q000378:metabolism*
D002135:Calcium-Binding Proteins; D018832:Molecular Chaperones; D009687:Nuclear Proteins; D037281:Calnexin; C087341:calmegin; D004247:DNA; D015500:Chloramphenicol O-Acetyltransferase