title: Effect of mild malnutrition on disaccharidase activity and glucose uptake in intestinal brush border vesicles of growing monkeys.
abstract: BACKGROUND
  The effect of severe malnutrition has been reported by some workers, but there is a paucity of information on milder forms even though 85% of pre-school children show evidence of mild to moderate malnutrition in India.
  
  
  METHODS
  Mild to moderate protein energy malnutrition was induced in 12 monkeys, and 12 were kept as controls. After a 30-40% reduction in body weight as compared with their controls, the animals were killed, and brush border membrane vesicles were prepared from three small parts of the small intestine. Purified vesicles were used for the assay of lactase, sucrase, maltase and glucose uptake in vitro.
  
  
  RESULTS
  The activity of lactase, sucrase, and maltase, and uptake of (U-14C)-D-glucose was decreased in all three parts of small intestine, and the lactose tolerance test result was also abnormal in the protein energy-malnourished group. On nutritional rehabilitation, the disaccharidase activities recovered completely.
  
  
  CONCLUSIONS
  This study indicates that even mild to moderate malnutrition decreases the digestion and absorption of carbohydrates throughout the small intestine, and it should not be ignored during the developing stage.
authors.affiliation: Dept. of Gastroenterology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: S V
authors.initials: SV
authors.lastname: Rana
authors.forename: D
authors.initials: D
authors.lastname: Gupta
authors.forename: K
authors.initials: K
authors.lastname: Vaiphei
authors.forename: S
authors.initials: S
authors.lastname: Bhardwaj
authors.forename: S K
authors.initials: SK
authors.lastname: Mehta
mesh_terms: D000818:Animals; D004186:Disaccharidases / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D007408:Intestinal Absorption / Q000502:physiology; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000648:ultrastructure; D007421:Intestine, Small / Q000378:metabolism* / Q000648:ultrastructure; D007789:Lactose Tolerance Test; D008253:Macaca mulatta; D008871:Microvilli / Q000378:metabolism; D011502:Protein-Energy Malnutrition / Q000378:metabolism*
chemical_list: D004186:Disaccharidases; D005947:Glucose