Development of new nephrons in adult kidneys following gentamicin-induced nephrotoxicity.
Gentamicin-induced nephrotoxicity results in necrosis of proximal tubular epithelial cells. In mammals, the injured nephron can be repaired by migration and division of surviving cells. We examined this repair process in the fresh-water goldfish, Carassius auratus. Fish were given a single intraperitoneal injection of 50 mg/kg gentamicin and sacrificed at 1, 4, 14, and 21 days. Injured nephrons showed regeneration along the basement membrane several days following gentamicin administration. In addition, 2-3 weeks following the injection, entirely new nephrons formed in the renal interstitium. Development of new nephrons in adult fish kidneys provides an excellent model for studying renal development and toxicity, and could provide insights into new therapies for chronic renal disease.
Reimschuessel
R
R
Department of Pathology, University of Maryland School of Medicine, Baltimore 21201-1192, USA.
Williams
D
D
D000704:Analysis of Variance; D000818:Animals; D002470:Cell Survival; D004195:Disease Models, Animal; D005839:Gentamicins / Q000009:adverse effects*; D006054:Goldfish; D007150:Immunohistochemistry; D007683:Kidney Tubular Necrosis, Acute / Q000139:chemically induced* / Q000473:pathology*; D009399:Nephrons / Q000166:cytology / Q000502:physiology*; D012038:Regeneration
D005839:Gentamicins