Human muscle macrophages express beta-amyloid precursor and prion proteins and their mRNAs.
Well-characterized antibodies against beta-amyloid precursor protein (beta APP) and prion protein (PrP), and specific cRNA probes, were used to localize beta APP and PrP and their mRNAs in human muscle macrophages. Macrophages present in muscle biopsies of 51 patients with various neuromuscular disorders showed accumulation of beta APP and PrP, and strongly expressed beta APP and PrP mRNAs. These were present in all muscle macrophages unrelated to their localization within the muscle tissue or diagnosis. Our study provides the first demonstration that human muscle resident macrophages synthesize and accumulate beta APP and PrP. We suggest that those proteins play a role in biology of muscle macrophages, including their participation in inflammatory and immune responses.
Askanas
V
V
Department of Neurology, University of Southern California School of Medicine, Los Angeles 90017, USA.
Sarkozi
E
E
Bilak
M
M
Alvarez
R B
RB
Engel
W K
WK
D000293:Adolescent; D000328:Adult; D000368:Aged; D016564:Amyloid beta-Protein Precursor / Q000096:biosynthesis*; D002648:Child; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008264:Macrophages / Q000378:metabolism*; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009132:Muscles / Q000166:cytology / Q000378:metabolism*; D011328:Prions / Q000096:biosynthesis*; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis*
D016564:Amyloid beta-Protein Precursor; D011328:Prions; D015347:RNA Probes; D012333:RNA, Messenger