title: Immunolocalization of the neural cell adhesion molecule L1 in non-proliferating epithelial cells of the male urogenital tract.
abstract: The localization of the neural cell adhesion molecule L1 in the male urogenital tract (including seminal vesicles and prostate) of the mouse and bull was investigated using immunocytochemical and immunochemical methods in order to better understand the function of this glycoprotein in non-neural tissues. L1 antibodies labeled non-myelinated nerves in all portions of the urogenital tract investigated. However, L1 immunoreactivity was also found between epithelial cells of several regions of the urogenital system including epididymal tail, deferent duct, ejaculatory duct and seminal vesicles. Some L1 immunoreactivity was also demonstrated between epithelial cells of murine urinary bladder and urethra. The specificity of the immunoreaction was verified by western blots. There was no correlation between L1 expression and proliferating activity as revealed by double immunocytochemistry using various markers of cell proliferation. This unexpected expression of L1 in nonneural tissues is mainly restricted to non-proliferating epithelia of those portions of the urogenital tract that are derived from the Wolffian duct. It is suggested that L1 in these epithelia could enhance the mechanical resistance and reduce transepithelial permeability.
authors.affiliation: Institut für Anatomie, Universität Regensburg, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Kujat
authors.forename: F
authors.initials: F
authors.lastname: Miragall
authors.forename: D
authors.initials: D
authors.lastname: Krause
authors.forename: R
authors.initials: R
authors.lastname: Dermietzel
authors.forename: K H
authors.initials: KH
authors.lastname: Wrobel
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D015816:Cell Adhesion Molecules, Neuronal / Q000032:analysis*; D002455:Cell Division; D004822:Epididymis / Q000378:metabolism* / Q000648:ultrastructure; D007150:Immunohistochemistry; D039841:Leukocyte L1 Antigen Complex; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011467:Prostate / Q000378:metabolism*; D012669:Seminal Vesicles / Q000378:metabolism*
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D039841:Leukocyte L1 Antigen Complex; D018809:Proliferating Cell Nuclear Antigen