Expression and purification of Clostridium perfringens beta-toxin glutathione S-transferase fusion protein.
The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid difference between the two proteins. The purified beta-toxin fusion protein is not toxic in mice, but rabbit antiserum raised against it neutralises the toxic effect of C. perfringens type C culture filtrate in mice.
Steinthorsdottir
V
V
Institute for Experimental Pathology, University of Iceland, Reykjavík.
Fridriksdottir
V
V
Gunnarsson
E
E
Andrésson
O S
OS
Steinporsdóttir
Frithriksdóttir
V
V
D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D003016:Clostridium perfringens / Q000472:pathogenicity*; D005982:Glutathione Transferase / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*
D001427:Bacterial Toxins; C031884:CPB protein, Clostridium perfringens; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase