Fos immunoreactivity assessment on human normal and pathological bronchial biopsies.
The transcription factor Fos is involved in cell proliferation and differentiation. Its expression in normal and pathological adult human tissues and cells has rarely been studied. We therefore studied bronchial biopsies obtained from 14 normal subjects (NS), 18 non-steroid-treated asthmatics, 10 corticosteroid-treated asthmatics and 10 patients with chronic bronchitis (CB), in addition to 34 patients with lung cancer (LC), by immunofluorescence for Fos immunoreactivity, using a highly specific polyclonal antibody. Bronchial tissue of 0/10 NS, 11/18 non-steroid-treated asthmatics, 1/10 steroid-treated asthmatics, 0/10 CB and 1/34 LC expressed Fos. In asthmatic patients, the expression was heterogeneous, localized to epithelial cells and correlated with the epithelium shedding (tau = 0.45, P = 0.0001). Corticosteroid-treated patients rarely expressed Fos, suggesting a role for this proto-oncogene in asthmatic bronchial inflammation. Fos was rarely expressed in the normal and pathological (CB, LC) proliferative compartment of the human bronchi, suggesting its low role in cell proliferation of the large airways.
Demoly
P
P
Clinique des Maladies Respiratoires-CJF. INSERM 92-10, Hôpital Arnaud de Villeneuve, Centre Hospitalier Universitaire, Montpellier, France.
Chanez
P
P
Pujol
J L
JL
Gauthier-Rouvière
C
C
Michel
F B
FB
Godard
P
P
Bousquet
J
J
D000230:Adenocarcinoma / Q000737:chemistry; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001249:Asthma / Q000188:drug therapy; D001507:Beclomethasone / Q000627:therapeutic use; D001980:Bronchi / Q000737:chemistry*; D001991:Bronchitis; D019819:Budesonide; D018288:Carcinoma, Small Cell / Q000737:chemistry; D002294:Carcinoma, Squamous Cell / Q000737:chemistry; D002908:Chronic Disease; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008175:Lung Neoplasms / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D011282:Pregnenediones / Q000627:therapeutic use; D000090063:Proto-Oncogene Mas; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis*
C000715470:MAS1 protein, human; D011282:Pregnenediones; D000090063:Proto-Oncogene Mas; D016760:Proto-Oncogene Proteins c-fos; D019819:Budesonide; D001507:Beclomethasone