title: Expression of leukocyte chemotactic cytokines in myocardial tissue.
abstract: Cytotoxic action of leukocytes may be involved in the pathogenesis of inflammatory heart muscle disorders. We investigated the expression of rat leukocyte chemotactic cytokines--cytokine induced neutrophil chemoattractant (CINC) and JE--in cultured neonatal rat cardiac myocytes; CINC belongs to the interleukin 8 (IL-8) family and JE is a homologue of human monocyte chemoattractant protein 1 (MCP-1). In Northern blot analysis, CINC and JE transcripts were not clearly observed in unstimulated rat cardiac myocytes. However, their expression was clearly observed after exposure to tumour necrosis factor-alpha (TNF-alpha; 100 U/ml) for 2-6 h. We then evaluated IL-8 and MCP-1 mRNA expression in human endomyocardial biopsy specimens from seven patients with idiopathic dilated cardiomyopathy by polymerase chain reaction analysis. Both IL-8 and MCP-1 mRNA transcripts were recognized in all patients studied. These results show for the first time that leukocyte chemotactic cytokines, IL-8 and MCP-1, are expressed in myocardial tissue, which might contribute to the pathogenesis of inflammatory heart muscle disorders.
authors.affiliation: Department of Cardiology, Jichi Medical School, Tochigi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Seino
authors.forename: U
authors.initials: U
authors.lastname: Ikeda
authors.forename: H
authors.initials: H
authors.lastname: Sekiguchi
authors.forename: M
authors.initials: M
authors.lastname: Morita
authors.forename: K
authors.initials: K
authors.lastname: Konishi
authors.forename: T
authors.initials: T
authors.lastname: Kasahara
authors.forename: K
authors.initials: K
authors.lastname: Shimada
mesh_terms: D000818:Animals; D001483:Base Sequence; D018932:Chemokine CCL2; D002630:Chemotactic Factors / Q000096:biosynthesis*; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D016207:Cytokines / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018932:Chemokine CCL2; D002630:Chemotactic Factors; D016207:Cytokines; D016209:Interleukin-8