Identification and analysis of a 'young' polymorphic Alu element.
A polymorphic Alu element belonging to a young subfamily of Alu repeats has been identified. Sequence analysis showed that this Alu element is flanked by perfect direct repeats and a 3' oligo(dA)-rich tail. The Alu element, designated A25, is deleted by 34 nucleotides at the 5' end and has a single CpG mutation compared to the human-specific consensus sequence. Using a PCR-based assay, we demonstrated that the A25 Alu repeat is localized to human chromosome 8 and is polymorphic in humans.
Arcot
S S
SS
Human Genome Center, Lawrence Livermore National Laboratory, CA 94551, USA.
Fontius
J J
JJ
Deininger
P L
PL
Batzer
M A
MA
D001435:Bacteriophages / Q000378:metabolism; D001483:Base Sequence; D005787:Gene Frequency; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*