GM-CSF rapidly primes mice for enhanced cytokine production in response to LPS and TNF.
Subcutaneous administration of Granulocyte-macrophage-colony stimulating factor (GM-CSF) to mice enhanced LPS-induced cytokine production in the circulation (TNF, IL-6) and peritoneal cavity (IL-1 beta, IL-6). This effect was induced rapidly (within 1 h) and persisted for 4 h. Mice made leukopenic with cyclophosphamide retained the ability to respond to GM-CSF. In addition, TNF induced IL-6 production was also enhanced. These results demonstrate that GM-CSF has a pronounced priming effect on cytokine producing cells in vivo and raises the possibility that this cytokine may contribute to the pathogenesis of septic shock.
Brissette
W H
WH
Department of Immunology and Infectious Diseases, Pfizer Inc., Groton, CT 06340, USA.
Baker
D A
DA
Stam
E J
EJ
Umland
J P
JP
Griffiths
R J
RJ
D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D015850:Interleukin-6 / Q000096:biosynthesis; D007970:Leukopenia / Q000097:blood / Q000139:chemically induced; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000494:pharmacology*
D016207:Cytokines; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D016178:Granulocyte-Macrophage Colony-Stimulating Factor