title: Cloning and sequencing of a gene from the archaeon Pyrococcus furiosus with high homology to a gene encoding phosphoenolpyruvate synthetase from Escherichia coli.
abstract: A gene from the hyperthermophilic archaeon Pyrococcus furiosus, strain Vc1 (DSM 3638), contains an 817-amino-acid open reading frame which shows 42% identity to the phosphoenolpyruvate (PEP) synthetase of Escherichia coli. This putative P. furiosus PEP synthetase is slightly larger than the E. coli enzyme, the region between residues 58 and 89 being absent from the latter.
authors.affiliation: Department of Biochemistry and Molecular Biology, University College London, UK.
authors.forename: C E
authors.initials: CE
authors.lastname: Jones
authors.forename: T M
authors.initials: TM
authors.lastname: Fleming
authors.forename: P W
authors.initials: PW
authors.lastname: Piper
authors.forename: J A
authors.initials: JA
authors.lastname: Littlechild
authors.forename: D A
authors.initials: DA
authors.lastname: Cowan
mesh_terms: D000595:Amino Acid Sequence; D001105:Archaea / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017866:Phosphotransferases (Paired Acceptors) / Q000096:biosynthesis / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D017866:Phosphotransferases (Paired Acceptors); C022509:pyruvate, water dikinase