title: Lipids are covalently attached to rigid corneocyte protein envelopes existing predominantly as beta-sheets: a solid-state nuclear magnetic resonance study.
abstract: C solid-state nuclear magnetic resonance at natural abundance was used to study isolated corneocyte envelopes from porcine stratum corneum. The presence of lipids covalently attached to the protein envelopes was detected by chemical shifts of methylene and methyl groups of the bound lipids. The corneocyte protein envelopes are rigid, as suggested by efficient 1H to 13C cross polarization and 13C spin-lattice relaxation studies. The chemical shift of the carbonyl carbons of the protein envelopes supports the prediction that the chemically bound lipid envelope is attached to proteins arranged predominantly in the beta-sheet conformation, allowing a dense palisade of ceramide molecules to form a water-impermeable external sheath.
authors.affiliation: Marshall Research Laboratories, Department of Dermatology, University of Iowa College of Medicine, Iowa City 52242, USA.
authors.forename: N D
authors.initials: ND
authors.lastname: Lazo
authors.forename: J G
authors.initials: JG
authors.lastname: Meine
authors.forename: D T
authors.initials: DT
authors.lastname: Downing
mesh_terms: D000818:Animals; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D050356:Lipid Metabolism; D009682:Magnetic Resonance Spectroscopy; D011506:Proteins / Q000378:metabolism*; D013552:Swine
chemical_list: D011506:Proteins