Mapping of linear epitopes of the regulatory proteins of human T cell lymphotropic virus (HTLV) type II: identification of an HTLV-IIb-restricted epitope.
Epitope mapping analyses using synthetic peptides representing the RexII and TaxII proteins identified predominant seroreactivity to the carboxyl terminus of the HTLV-IIG12 TaxII protein (G12Tax 22-G12Tax24, amino acids [aa] 312-356). Moderate reactivity to only 1 RexII peptide (G12Rex9, aa 121-140) was found, while all other RexII and TaxII peptides exhibited minimal reactivity. Peptide G12Tax24 (aa 337-356) corresponded to the extended portion of the TaxII protein characteristic of HTLV-IIb viruses and appeared to represent an HTLV-IIb-restricted epitope. This study showed that this peptide can be used in immunoassays as a quick, simple serologic tool for assessing the minimal number of HTLV-IIb viruses present within specific populations, especially when genomic DNA is not available.
Pardi
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Retrovirus Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Coligan
J E
JE
Lal
R B
RB
D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D016022:Case-Control Studies; D016370:Gene Products, rex / Q000276:immunology*; D016356:Gene Products, tax / Q000276:immunology*; D015491:HTLV-II Infections / Q000276:immunology / Q000821:virology; D015367:Human T-lymphotropic virus 2 / Q000276:immunology*; D016056:Immunodominant Epitopes; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D016370:Gene Products, rex; D016356:Gene Products, tax; C094779:HTLV-II(G12) TaxII protein (337-356); D016056:Immunodominant Epitopes; D010446:Peptide Fragments