Reperfusion-induced arrhythmias and lethality are reduced by a 2KDa heparin fragment.
The influence of a low molecular weight heparin (Oligo-H, m.w. 2KDa) on ventricular arrhythmias and lethality induced by heart reperfusion following a 5 min coronary occlusion was studied in anesthetized rats. Both intravenous (i.v.) and subcutaneous (s.c.) injection of the compound dose- and time-dependently prevented the reperfusion syndrome: in all saline-pretreated animals post-ischemic reperfusion induced ventricular tachycardia (VT), which degenerated into ventricular fibrillation (VF) in 25 out of 30 rats, with a mortality rate of 73%; on the other hand, in rats i.v. or s.c. pretreated with Oligo-H (20 mg/kg, 30 and 90 min, respectively, before coronary occlusion), VT occurred in 4 out of 10-11 animals and degenerated into VF in 2-3 out of 10-11 animals, with a mortality rate of 18-20%. Even more effective was a low molecular weight dermatan sulfate (Oligo-DS, m.w. 2.1KDa). In rats treated with lidocaine, used as reference compound, at the dose of 5 mg/kg i.v. 10 min before coronary occlusion, VT occurred in 2 out of 10 animals and degenerated into VF in 1 out of 10 animals, with a mortality rate of 10%. It is concluded that low molecular weight glycosaminoglycans significantly reduce the consequences of heart reperfusion.
Guarini
S
S
Department of Biomedical Sciences, University of Modena, Italy.
Martini
M C
MC
Bertolini
A
A
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000209:etiology / Q000401:mortality / Q000517:prevention & control*; D003871:Dermatan Sulfate / Q000008:administration & dosage / Q000627:therapeutic use; D005260:Female; D006352:Heart Ventricles; D006493:Heparin / Q000008:administration & dosage / Q000627:therapeutic use*; D007275:Injections, Intravenous; D007279:Injections, Subcutaneous; D008297:Male; D008970:Molecular Weight; D015428:Myocardial Reperfusion Injury / Q000401:mortality / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D014693:Ventricular Fibrillation / Q000517:prevention & control
D003871:Dermatan Sulfate; D006493:Heparin