title: Structural and functional comparison of trematode haemoglobins.
abstract: Haemoglobins from three different species of trematodes (Gastrothylax crumenifer, Paramphistomum epiclitum and Isoparorchis hypselobagri) and their respective hosts were digested with papain. Peptides so produced were separated on 10-15% SDS-PAGGE. The probability of coincidence of the peptides was analysed statistically. Oxygen affinity curves of trematode haemoglobins were found to be a rectangular hyperbolic. The Hill coefficient values of all the trematode haemoglobins were found to be 1. The P50 values, at 25 degrees C in 0.2M phosphate buffer pH 7.4, ranged from 0.8 to 1.6 mmHg.
authors.affiliation: Department of Zoology, Aligarh Muslim University, India.
authors.forename: K A
authors.initials: KA
authors.lastname: Rashid
authors.forename: M
authors.initials: M
authors.lastname: Haque
authors.forename: A H
authors.initials: AH
authors.lastname: Siddiqi
mesh_terms: D000818:Animals; D006454:Hemoglobins / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D010108:Oxyhemoglobins / Q000378:metabolism*; D010206:Papain; D010446:Peptide Fragments / Q000737:chemistry; D011336:Probability; D013045:Species Specificity; D014200:Trematoda / Q000378:metabolism*
chemical_list: D006454:Hemoglobins; D010108:Oxyhemoglobins; D010446:Peptide Fragments; D010206:Papain