[Immunocytochemical analysis of human prolactin receptors using anti-idiotypic antibodies in human breast cancer].
In this study, we have prepared antiidiotypic antibodies specific of human prolactin receptors (PRL-R) in order to localize these receptors in breast cancer. Antiidiotypic antibodies were prepared using anti-human prolactin (anti-hPRL) sera obtained from New Zealand rabbits. 25 breast cancer were PRL-R positive using radio receptor assay. For immunohistochemistry, the tissues fixed with Nakane and treated by NH4Cl and Saponin to improve staining specificity, were incubated with antiidiotypic antibodies (1/100e). Cytoplasm and membrane of human breast cancer cells were PRL-R positive in 20/25 breast cancer with antiidiotypic antibodies. There was no staining with preimmune serum and immunohistochemical staining elimination by prior absorption with anti-hPRL antibodies.
Leroy-Martin
B
B
Laboratoire d'Histologie-Embryologie et Biologie de la Reproduction, Faculté de Médecine, Lille.
Peyrat
J P
JP
Amrani
S
S
Lorthioir
M
M
Leonardelli
J
J
D000888:Antibodies, Anti-Idiotypic; D000918:Antibody Specificity; D001943:Breast Neoplasms / Q000175:diagnosis*; D006801:Humans; D007150:Immunohistochemistry; D011237:Predictive Value of Tests; D011981:Receptors, Prolactin / Q000032:analysis*
D000888:Antibodies, Anti-Idiotypic; D011981:Receptors, Prolactin
Analyse immunocytochimique des récepteurs prolactiniques (R-PRL) humains à l'aide d'anticorps antiidiotypes dans les cancers du sein humain.