Heterogeneity in p53 mutations in mouse mammary tumor subpopulations with different metastatic potential from the orthotopic site.
We have found that p53 expression is altered with progression to the metastatic phenotype of a series of neoplastic subpopulations of a single mouse mammary tumor. Single strand conformation polymorphism (SSCP) analysis of p53 transcripts indicate that the expressed p53 genes in each of the subpopulations contain one or more differences from wild type p53. Although the mutations in the coding sequence of p53 are different in each of the sublines, suggesting that independent mutational events have occurred, the mutations are clustered in exons 2-4 and in exon 6. In the metastatic subpopulations, there is either a complete loss of p53 transcriptional activity or accumulation of transcripts with an additional alteration in exons 4-5.
Shekhar
P V
PV
Breast Cancer Program, Michigan Cancer Foundation, Meyer L. Prentis Comprehensive Cancer Center of Metropolitan Detroit 48201, USA.
Werdell
J
J
Christman
J K
JK
Miller
F R
FR
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004262:DNA Restriction Enzymes; D016158:Genes, p53; D008325:Mammary Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D009154:Mutation; D009362:Neoplasm Metastasis; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping
D017931:DNA Primers; D004262:DNA Restriction Enzymes