title: Combined FISH painting and harlequin staining for cell cycle-controlled chromosome analysis in human lymphocytes.
abstract: We present a simple method that allows scoring of FISH-painted chromosomes exclusively in the first division human lymphocytes. It consists of a combination of FISH with chromosome-specific libraries and a differential sister chromatid staining after BrdU treatment of lymphocyte cultures. The method allows a precise quantification of induced chromosome damage for human biodosimetry.
authors.affiliation: Institut für Strahlenbiologie, GSF-Forschungszentrum für Umwelt und Gesundheit Neuherberg, Oberschleissheim, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Kulka
authors.forename: R
authors.initials: R
authors.lastname: Huber
authors.forename: P
authors.initials: P
authors.lastname: Müller
authors.forename: S
authors.initials: S
authors.lastname: Knehr
authors.forename: M
authors.initials: M
authors.lastname: Bauchinger
mesh_terms: D002453:Cell Cycle; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000648:ultrastructure*