Purification of Paracoccus denitrificans cytochrome c552 and sequence analysis of the gene.
Unlike mitochondria, many bacteria use a large repertoire of c-type cytochromes in different branches of their electron transport system. Among the many cytochromes c present in the soil bacterium Paracoccus denitrificans, a membrane-bound cytochrome (c552) has been suggested to mediate the electron transport between the cytochrome bc1 complex and cytochrome-c oxidase [Berry, E. A. & Trumpower, B. L. (1985) J. Biol. Chem. 260, 2458-2467]. We have purified this cytochrome from cytoplasmic membranes, and cloned and sequenced its gene, cycM. Sequence analysis reveals that, while its C-terminal portion is highly similar to type-I cytochromes c, its N-terminal part contains a hydrophobic segment providing membrane attachment. In addition, we present immunological evidence for its functional role in respiration.
Turba
A
A
Biozentrum Molekulare Genetik, Johann Wolfgang Goethe-Universität, Frankfurt, Germany.
Jetzek
M
M
Ludwig
B
B
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003574:Cytochrome c Group / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial; D004579:Electron Transport; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial; D007106:Immune Sera; D008969:Molecular Sequence Data; D010231:Paracoccus denitrificans / Q000201:enzymology* / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D003574:Cytochrome c Group; D004269:DNA, Bacterial; D007106:Immune Sera; C083951:cytochrome c553; C022302:cytochrome C-552