A relatively small 5' regulatory region of esterase S gene of Drosophila virilis determines the specific expression as revealed in transgenic experiments.
Expression of the esterase S gene of Drosophila virilis was studied in transgenic experiments. Truncated genomic copy of this gene including 400 bp of 5' regulatory region was integrated into the genome of Drosophila melanogaster. The products of the transferred gene were detected. It was found that strict temporal and tissue specificity of the esterase S gene expression is conserved in transformed flies. The results suggest that this specificity is evidently determined by the regulatory region of the esterase S gene and controlled by cis mechanism.
Korochkin
L I
LI
Institute of Gene Biology, Russian Academy of Sciences, Moscow.
Panin
V M
VM
Pavlova
G V
GV
Kopantseva
M R
MR
Shostak
N G
NG
Bashkirov
V N
VN
Gabitova
L B
LB
Sergeev
P V
PV
D000818:Animals; D030801:Animals, Genetically Modified; D043182:Carboxylesterase; D002265:Carboxylic Ester Hydrolases / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics*; D002874:Chromosome Mapping; D015342:DNA Probes; D004330:Drosophila / Q000201:enzymology* / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D017344:Genes, Insect; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping
D015342:DNA Probes; D029721:Drosophila Proteins; D002265:Carboxylic Ester Hydrolases; D043182:Carboxylesterase; C469609:esterase-S, Drosophila