title: Randomly amplified polymorphic DNA analysis of Xylella fastidiosa Pierce's disease and oak leaf scorch pathotypes.
abstract: Randomly amplified polymorphic DNA analysis was conducted with 14 primers to 17 strains of Xylella fastidiosa. There was a high degree of similarity among the seven Pierce's disease (PD) strains (Sxy > 0.93) and the seven oak leaf scorch (OLS) strains (Sxy > 0.96). However, the two groups were different, with a similarity index of 0.67, confirming the presence of a PD DNA cluster and suggesting the presence of a new OLS cluster. The control plum leaf scald strains (two strains) together with the periwinkle wilt strain had a much smaller similarity index (0.44) compared with the PD and OLS clusters.
authors.affiliation: Center for Viticultural Science, Florida A&M University, Tallahassee 32307, USA.
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: O
authors.initials: O
authors.lastname: Lamikanra
authors.forename: C J
authors.initials: CJ
authors.lastname: Chang
authors.forename: D L
authors.initials: DL
authors.lastname: Hopkins
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial