Dexamethasone-induced changes in glucose transporter 4 in rat heart muscle, skeletal muscle and adipocytes.
To clarify the effect of glucocorticoid on glucose transporters (GLUT) in adipocytes and muscle, we examined the changes of GLUT4 in rat heart muscle, skeletal muscle and adipocytes during long-term administration of dexamethasone and the translocation of GLUT4. The levels of GLUT4 in the plasma membrane and the low-density microsome fraction were measured by Western blotting using anti-GLUT4 peptide antibody. The levels of GLUT4 in the heart and skeletal muscles of rat were unchanged by treatment of dexamethasone. In the adipocytes the level of GLUT4 in plasma membrane was changed, but it was decreased in the low-density microsome fraction. Although adipocytes are less involved in blood sugar regulation than skeletal muscle, this finding suggests that glucose metabolism in Cushing's syndrome is affected partly by a decrease of GLUT4 in the adipocytes.
Oda
N
N
Department of Internal Medicine, Fujita Health University School of Medicine, Aichi, Japan.
Nakai
A
A
Mokuno
T
T
Sawai
Y
Y
Nishida
Y
Y
Mano
T
T
Asano
K
K
Itoh
Y
Y
Kotake
M
M
Kato
S
S
D015720:5'-Nucleotidase / Q000032:analysis; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D000818:Animals; D000918:Antibody Specificity; D001786:Blood Glucose / Q000032:analysis; D015153:Blotting, Western; D001835:Body Weight / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology*; D005700:Galactosyltransferases / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D005951:Glucose Tolerance Test; D051275:Glucose Transporter Type 4; D006321:Heart / Q000187:drug effects; D007328:Insulin / Q000097:blood; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D009004:Monosaccharide Transport Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D009124:Muscle Proteins; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D001786:Blood Glucose; D051275:Glucose Transporter Type 4; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; C495694:Slc2a4 protein, rat; D003907:Dexamethasone; D005700:Galactosyltransferases; D015720:5'-Nucleotidase; D005947:Glucose