A single cytochrome P-450 system is involved in degradation of the herbicides EPTC (S-ethyl dipropylthiocarbamate) and atrazine by Rhodococcus sp. strain NI86/21.
During atrazine degradation by Rhodococcus sp. strain N186/21, N-dealkylated metabolites and an hydroxyisopropyl derivative are produced. The cytochrome P-450 system that is involved in degradation of thiocarbamate herbicides by strain N186/21 (I. Nagy, G. Schoofs, F. Compernolle, P. Proost, J. Vanderleyden, and R. De Mot, J. Bacteriol. 177:676-687, 1995) is also required for atrazine degradation. Atrazine-degrading activity was conferred on the atrazine-negative strains, mutant FAJ2027 of Rhodococcus sp. strain N186/21 and Rhodococcus erythropolis SQ1, upon transformation with the genes encoding the cytochrome P-450 system.
Nagy
I
I
Department of Applied Plant Sciences, Catholic University of Leuven, Heverlee, Belgium.
Compernolle
F
F
Ghys
K
K
Vanderleyden
J
J
De Mot
R
R
D000478:Alkylation; D001280:Atrazine / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism*; D004790:Enzyme Induction; D006540:Herbicides / Q000378:metabolism*; D012240:Rhodococcus / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D012989:Soil Pollutants / Q000378:metabolism*; D013859:Thiocarbamates / Q000378:metabolism*
D001426:Bacterial Proteins; D006540:Herbicides; D012989:Soil Pollutants; D013859:Thiocarbamates; D003577:Cytochrome P-450 Enzyme System; D001280:Atrazine; C036251:EPTC