title: Effects of intravenous anesthetics on phosphatidylinositol turnover in rat cerebral cortical prisms.
abstract: Noradrenergic pathways in the brain have been thought to be related to the site of anesthetic action. Norepinephrine (NE) in the central nervous system stimulates phosphatidylinositol (PI) turnover through alpha 1-adrenergic receptors. The present study was designed to examine the effects of intravenous anesthetics on NE-induced PI turnover in rat cerebral cortical prisms. NE-induced inositol monophosphate (IP1) formation was inhibited by droperidol (dose for 50% inhibition [ID50], 0.0258 +/- 0.00023 microM [mean +/- SE]), fentanyl (2.36 +/- 0.0017), diazepam (201 +/- 2.12), and thiamylal (231 +/- 1.94) in a dose-dependent manner, but was not affected by ketamine. Naloxone or flumazenil did not attenuate the inhibitory effect of fentanyl or diazepam on NE-induced IP1 formation. The results suggest that these effects on the PI turnover in the cortex may be related to their pharmacologic properties including the anesthetic action.
authors.affiliation: Department of Anesthesiology, Nagasaki University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Makita
authors.forename: O
authors.initials: O
authors.lastname: Shibata
authors.forename: T
authors.initials: T
authors.lastname: Tsujita
authors.forename: T
authors.initials: T
authors.lastname: Fujigaki
authors.forename: H
authors.initials: H
authors.lastname: Nakamura
authors.forename: K
authors.initials: K
authors.lastname: Sumikawa
mesh_terms: D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism; D003975:Diazepam / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004329:Droperidol / Q000494:pharmacology; D005283:Fentanyl / Q000494:pharmacology; D005442:Flumazenil / Q000494:pharmacology; D007295:Inositol Phosphates / Q000096:biosynthesis*; D007649:Ketamine / Q000494:pharmacology; D008297:Male; D009270:Naloxone / Q000494:pharmacology; D009638:Norepinephrine / Q000502:physiology; D010716:Phosphatidylinositols / Q000378:metabolism*; D011224:Prazosin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013840:Thiamylal / Q000494:pharmacology
chemical_list: D018686:Anesthetics, Intravenous; D007295:Inositol Phosphates; D010716:Phosphatidylinositols; D013840:Thiamylal; C002647:inositol 1-phosphate; D009270:Naloxone; D005442:Flumazenil; D007649:Ketamine; D004329:Droperidol; D003975:Diazepam; D005283:Fentanyl; D009638:Norepinephrine; D011224:Prazosin