An alternative view of T-cell receptor-MHC interaction: T-cell receptor binds transversally to the alpha-helices of the MHC molecule.
We have attempted to elucidate the relative orientation of the T-cell receptor (TCR) to the major histocompatibility complex (MHC)-antigen complex during antigen recognition, using the T-cell response to B10.A (I-Ek) and B10.A(5R) (I-Eb) mice to the 1-23(H) peptide derived from glycoprotein D of the herpes simplex virus. The 1-23(H)-specific T-cells derived from both B10.A and B10.A(5R) mice use the same set of V alpha genes and a different array of V beta genes. The CDR1s of these TCR beta chains share residues at particular positions. The CDR2s of the TCR beta chains have a negative charge, which correlates with I-Eb reactivity and with the positively charged polymorphic residues residing at the C-terminal end of the alpha-helix of the I-Eb beta chain of the class II molecule. Taken together, the data suggest that the TCR beta chain interacts with both the alpha and beta chains of the MHC class II molecule, as does the TCR alpha chain.
Tang
X X
XX
Wistar Institute, Philadelphia, PA 19104, USA.
Ikegaki
N
N
Danska
J S
JS
Heber-Katz
E
E
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D003574:Cytochrome c Group / Q000737:chemistry / Q000276:immunology; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D015332:Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D007477:Ions; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D009036:Moths; D011110:Polymorphism, Genetic; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D018139:Simplexvirus / Q000276:immunology*; D013329:Structure-Activity Relationship; D013601:T-Lymphocytes / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000276:immunology* / Q000378:metabolism
D000956:Antigens, Viral; D003574:Cytochrome c Group; D017931:DNA Primers; D000949:Histocompatibility Antigens Class II; C024138:I-E-antigen; D007477:Ions; D046911:Macromolecular Substances; D011948:Receptors, Antigen, T-Cell; D014759:Viral Envelope Proteins; C039447:glycoprotein D, Human herpesvirus 1