Characterization of a major Mycoplasma penetrans lipoprotein and of its gene.
A novel mycoplasmal species designated as Mycoplasma penetrans has been isolated recently from patients infected with human immunodeficiency virus. p35, a major antigen extracted from the membrane of this mycoplasma using Triton X-114 has been found to be a lipoprotein. After proteolytic treatment of p35, the sequence of one of the resulting peptides was determined and a corresponding oligonucleotide was deduced. Using this oligonucleotide as a probe the p35 gene was cloned and sequenced. Sequence analysis revealed an amino-terminal signal peptide with a potential acylation site which would result in a 35.3 kDa mature product. In addition, the p35 gene was followed by an open reading frame with a corresponding polypeptide partially homologous to p35, in particular to the N-terminus region.
Ferris
S
S
Institut Pasteur, Departement du SIDA et des Retrovirus, Unité d'Oncologie Virale et URA CNRS 1157, Paris, France.
Watson
H L
HL
Neyrolles
O
O
Montagnier
L
L
Blanchard
A
A
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001483:Base Sequence; D003001:Cloning, Molecular; D008074:Lipoproteins / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D018661:Mycoplasma penetrans / Q000737:chemistry*
D000942:Antigens, Bacterial; D008074:Lipoproteins