title: Primary structure and partial characterization of a life-cycle-regulated cysteine protease from Trypanosoma (Nannomonas) congolense.
abstract: Trypanosoma (Nannomonas) congolense is an important pathogenic parasite of domestic livestock in Africa. We have cloned a cDNA encoding a prepro-cysteine protease of this protozoan, the sequence of which indicates it is an early mRNA processing intermediate. Northern analysis demonstrates a life-cycle-stage specificity similar to previously described enzymatic data. The deduced amino-acid sequence shows extensive similarity to cysteine proteases of other parasitic protozoa, as well as papain and cathepsin L. As with other African trypanosomes, a poly-proline tract connects the catalytic domain with an unusual C-terminal extension.
authors.affiliation: International Laboratory for Research on Animal Diseases (ILRAD), Nairobi, Kenya.
authors.forename: W R
authors.initials: WR
authors.lastname: Fish
authors.forename: Z M
authors.initials: ZM
authors.lastname: Nkhungulu
authors.forename: C W
authors.initials: CW
authors.lastname: Muriuki
authors.forename: D M
authors.initials: DM
authors.lastname: Ndegwa
authors.forename: J D
authors.initials: JD
authors.lastname: Lonsdale-Eccles
authors.forename: J
authors.initials: J
authors.lastname: Steyaert
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D014348:Trypanosoma congolense / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
chemical_list: D018076:DNA, Complementary; D003546:Cysteine Endopeptidases