Gonadotropin-releasing hormone analog-induced patterns of luteinizing hormone secretion in female Wapiti (Cervus elaphus nelsoni) during the breeding season, anestrus, and pregnancy.
We conducted two studies to determine the pattern of GnRH analog-induced LH secretion in adult female wapiti during the breeding season, anestrus, and pregnancy. In the first study, we measured LH secretion during the breeding season and anestrus in five females challenged with each of five doses of GnRH analog (0.3, 1, 3, 10, and 30 micrograms/50 kg BW). In the second study, LH response was determined in six pregnant females treated with six doses of GnRH analog (0.5, 1, 2, 4, 8, and 16 micrograms/50 kg BW). Animals were fitted nonsurgically with indwelling jugular catheters, and blood samples were collected at 0, 30, 60, 90, 120, 180, 240, 300, 360, 420, and 480 min postinjection. Mean maximum serum concentrations of LH were not different (p = 0.45) between females treated during the breeding season (mean = 29.1 +/- 1.9 [SE] ng/ml) and anestrus (mean = 32.4 +/- 4.3 [SE] ng/ml); however, anestrous females required 3.3 times more GnRH analog (3 vs. 10 micrograms/50 kg BW) to produce the same response. Maximal inducible LH concentrations were lowest (mean = 7.7 +/- 2.5 [SE] ng/ml) for pregnant wapiti, and the magnitude of the response decreased exponentially (r2 = 0.98, p = 0.002, SEb = 0.65) during gestation. Although the magnitude of the response declined over time, maximal release consistently occurred at the same dose (2 micrograms/50 kg BW) (treatment x trial interaction, p = 0.53). We conclude from these studies that the amount of LH released in response to a given dose of GnRH analog depends on reproductive status.(ABSTRACT TRUNCATED AT 250 WORDS)
Baker
D L
DL
Colorado Division of Wildlife, Fort Collins 80526, USA.
Miller
M W
MW
Nett
T M
TM
D000780:Anestrus / Q000502:physiology*; D000818:Animals; D003670:Deer / Q000502:physiology*; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000378:metabolism*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011374:Progesterone / Q000097:blood; D012098:Reproduction / Q000502:physiology*; D012621:Seasons
D006728:Hormones; D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; C010661:surfagon; D007986:Luteinizing Hormone