An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding.
We have isolated a major integral membrane protein from Golgi-derived coatomer-coated vesicles. This 24-kDa protein, p24, defines a family of integral membrane proteins with homologs present in yeast and humans. In addition to sequence similarity, all p24 family members contain a motif with the characteristic heptad repeats found in coiled coils. When the yeast p24 isoform, yp24A, is knocked out in a strain defective for vesicle fusion, a dramatic reduction in the accumulation of transport vesicles is observed. Together, these results indicate a role for this protein family in the budding of coatamer-coated and other species of coated vesicles.
Stamnes
M A
MA
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
Craighead
M W
MW
Hoe
M H
MH
Lampen
N
N
Geromanos
S
S
Tempst
P
P
Rothman
J E
JE
D020727:ADP-Ribosylation Factors; D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000378:metabolism; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D016466:CHO Cells; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle; D020756:Coatomer Protein; D006224:Cricetinae; D003600:Cytosol / Q000378:metabolism; D017931:DNA Primers; D004285:Dogs; D019204:GTP-Binding Proteins / Q000378:metabolism; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D015388:Organelles / Q000502:physiology; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000502:physiology; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D020756:Coatomer Protein; D017931:DNA Primers; D008565:Membrane Proteins; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors