title: Cre-mediated site-specific translocation between nonhomologous mouse chromosomes.
abstract: Chromosome rearrangements, such as large deletions, inversions, or translocations, mediate migration of large DNA segments within or between chromosomes, which can have major effects on cellular genetic control. A method for chromosome manipulation would be very useful for studying the consequences of large-scale DNA rearrangements in mammalian cells or animals. With the use of the Cre-loxP recombination system of bacteriophage P1, we induced a site-specific translocation between the Dek gene on chromosome 13 and the Can gene on chromosome 2 in mouse embryonic stem cells. The estimated frequency of Cre-mediated translocation between the nonhomologous mouse chromosomes is approximately 1 in 1200-2400 embryonic stem cells expressing Cre recombinase. These results demonstrate the feasibility of site-specific recombination systems for chromosome manipulation in mammalian cells in vivo, breaking ground for chromosome engineering.
authors.affiliation: Department of Genetics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: J
authors.initials: J
authors.lastname: Van Deursen
authors.forename: M
authors.initials: M
authors.lastname: Fornerod
authors.forename: B
authors.initials: B
authors.lastname: Van Rees
authors.forename: G
authors.initials: G
authors.lastname: Grosveld
mesh_terms: D000818:Animals; D017101:Bacteriophage P1 / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D015321:Gene Rearrangement; D018390:Gene Targeting; D005821:Genetic Techniques; D005822:Genetic Vectors; D019426:Integrases; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013234:Stem Cells; D014178:Translocation, Genetic; D014764:Viral Proteins
chemical_list: D017931:DNA Primers; D014764:Viral Proteins; D004247:DNA; C045073:Cre recombinase; D004254:DNA Nucleotidyltransferases; D019426:Integrases