Stimulation by epigallocatechin gallate of interleukin-1 production by human peripheral blood mononuclear cells.
(-) Epigallocatechin gallate (EGCg) potently stimulated the production of interleukin-1 (IL-1) and tumor necrosis factor by human peripheral blood mononuclear cells. The intracellular amounts of IL-1 beta and especially IL-1 alpha induced by EGCg, were significantly higher than their extracellular counterparts. ECCg stimulated the production of adherent cells, with IL-1 producing capacity (per cell basis) that was significantly higher than nonadherent cells. Although IL-1 alpha mRNA synthesis (assessed by Reverse Transcriptase-Polymerase Chain Reaction) was slightly enhanced, IL-1 beta mRNA synthesis was not significantly enhanced by EGCg treatment. Lipopolysaccharide (LPS) also stimulated the production of IL-1 alpha and IL-1 beta production, but failed to induce the adherent cells. These data suggest that EGCg and LPS stimulate mononuclear cells by different mechanisms.
Sakagami
H
H
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Takeda
M
M
Sugaya
K
K
Omata
T
T
Takahashi
H
H
Yamamura
M
M
Hara
Y
Y
Shimamura
T
T
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001483:Base Sequence; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002478:Cells, Cultured; D017931:DNA Primers; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000097:blood; D007700:Kinetics; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000970:Antineoplastic Agents; D017931:DNA Primers; D007375:Interleukin-1; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D002392:Catechin; C045651:epigallocatechin gallate