[Studies on inactivation of hepatitis B virus with polymerase chain reaction].
Polymerase chain reaction-ethidium bromide (PCR-EB) method for detecting hepatitis B virus DNA (HBV-DNA) was established with good specificity and a detection limit of 1 pg HBV-DNA, minimum HBV infection dose in susceptible animal, chimpanzees, could be detected with it. Determination of inactivation of HBV-DNA could be inactivated with active chlorine 1,250 mg/L for 60 minutes, or 2,500 mg/L for 30 minutes, 10 pg HBV-DNA in purified Dane particles could be inactivated by active chlorine 625 mg/L for 10 minutes. Accordingly, use of PCR to evaluate the effects of chlorine disinfectant in inactivating HBV was feasible, and HBV-DNA was a more reliable index for inactivation of HBV than HBsAg.
Tai
C
C
Baoan Health and Anti-epiemic Station, Shenzhen.
Zhang
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C
D001483:Base Sequence; D002713:Chlorine / Q000494:pharmacology*; D004279:DNA, Viral / Q000032:analysis* / Q000187:drug effects; D004202:Disinfectants / Q000494:pharmacology*; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods
D004279:DNA, Viral; D004202:Disinfectants; D002713:Chlorine