The cattle major histocompatibility complex (MHC) class-I possesses HLA-like promoters.
To study the genetic regulation of the cattle major histocompatibility complex (MHC) class-I, a cattle MHC class-I promoter DNA fragment was isolated and characterized for the first time. Semi-degenerate PCR was performed on cattle genomic DNA and the resulting product was isolated, subcloned and sequenced. Sequence comparison of the HLA-A, -B and -C promoters to the cloned product, designated BL3-6prmtr, revealed the cattle MHC class-I promoter to have close homology to human MHC class-I promoters. To address the ability of the cattle MHC class-I promoter to initiate transcription, BL3-6prmtr was subcloned into a luciferase reporter vector and transiently transfected into cattle and human B-lymphoblastoid cell lines. A strong transcription initiation ability of BL3-6prmtr was observed, including the ability of the enhancer A and interferon response sequence (IRS) to upregulate transcription initiation.
Harms
J S
JS
Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison 53706, USA.
Li
W
W
Splitter
G A
GA
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000276:immunology; D004742:Enhancer Elements, Genetic; D005805:Genes, MHC Class I; D017930:Genes, Reporter; D006680:HLA Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014158:Transcription, Genetic; D014162:Transfection
D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D011993:Recombinant Fusion Proteins; D008156:Luciferases