Ontogenesis of rat liver microsomal glutathione transferase.
The ontogenesis of rat liver microsomal glutathione transferase was investigated by activity measurements and immunochemical methods. The activity rises from a very low level (3% of adults) at day 8 pre-partum to adult levels at days 50-150. Increases are associated with the neonatal and late-suckling clusters. Interestingly the capacity to become activated by N-ethylmaleimide is much lower in females early and late in life (days 35-100 and 300-550). After the initial increases (from 10% of adult levels at day 8 pre-partum), protein levels determined immunochemically remain constant throughout life with no apparent sex differences. The developmental pattern of microsomal glutathione transferase resembles those of other drug-metabolizing enzymes indicating that the function of the enzyme is required in adult life.
Lundqvist
G
G
Division of Toxicology, Karolinska Institutet, Stockholm, Sweden.
Morgenstern
R
R
D000375:Aging; D000818:Animals; D004137:Dinitrochlorobenzene / Q000378:metabolism; D005033:Ethylmaleimide; D005260:Female; D005333:Fetus; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000378:metabolism; D008099:Liver / Q000196:embryology / Q000254:growth & development; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D004137:Dinitrochlorobenzene; D005982:Glutathione Transferase; D005033:Ethylmaleimide