Molecular cloning of NKB1. A natural killer cell receptor for HLA-B allotypes.
The expression of certain MHC class I allotypes by potential target cells can inhibit NK cell-mediated cytotoxicity. We recently identified the NKB1 surface Ag, expressed on T and NK cell subsets, as a putative inhibitory receptor for HLA-B class I molecules possessing the Bw4 serologic epitope. NKB1 is a 70-kDa glycoprotein that after deglycosylation migrates as a 50-kDa protein as determined by SDS-PAGE. A cDNA encoding the NKB1 receptor was cloned from a NKB1+T cell cDNA library by expression in COS-7 cells using the anti-NKB1 mAb DX9. NKB1 is a member of the lg superfamily containing three lg-like domains in the extracellular region and is related to the recently identified family (p58/NKAT) of human NK and T cell surface molecules that appear to function as inhibitory receptors for HLA class I.
D'Andrea
A
A
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304, USA.
Chang
C
C
Franz-Bacon
K
K
McClanahan
T
T
Phillips
J H
JH
Lanier
L L
LL
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002999:Clone Cells; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis*; D015870:Gene Expression; D015235:HLA-B Antigens; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D011971:Receptors, Immunologic / Q000235:genetics*; D054340:Receptors, KIR; D054344:Receptors, KIR2DL3; D054341:Receptors, KIR3DL1; D054349:Receptors, KIR3DS1; D013601:T-Lymphocytes / Q000276:immunology
D018076:DNA, Complementary; D015235:HLA-B Antigens; C515078:KIR2DL3 protein, human; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D054340:Receptors, KIR; D054344:Receptors, KIR2DL3; D054341:Receptors, KIR3DL1; D054349:Receptors, KIR3DS1