Chromatographic evidence for Amadori product formation in rat liver aminophospholipids.
This work presents data which indicates the presence of Amadori product derived from Maillard reaction in aminophospholipids. The presence of 5-HMF, a stable derivative of acid-treated Amadori product, in the phospholipidic fraction from cell membranes was established by HPLC-UV and subsequent GC/MS analysis. The assay for 5-HMF in rat liver phospholipid revealed the presence of this molecule in membrane phospholipids of euglycemic rats, and showed increased glycation levels in membrane phospholipids from streptozotocin-induced diabetic rats (p < 0.001). This gives a new insight to cell membrane physiology and physiopathology.
Pamplona
R
R
Dpt. Basic Medical Sciences, Faculty of Medicine, University of Lleida, Spain.
Bellmunt
M J
MJ
Portero
M
M
Riba
D
D
Prat
J
J
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D015416:Maillard Reaction; D008563:Membrane Lipids / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley
D008563:Membrane Lipids; D010714:Phosphatidylethanolamines; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde