The amino terminus of Tax is required for interaction with the cyclic AMP response element binding protein.
Tax of human T-cell leukemia virus type 1 was analyzed for interaction with the cyclic AMP response element binding protein (CREB) in vitro with and without Tax response element DNA. Mutations in the carboxy terminus of Tax (L296G and L320G) did not affect binding to CREB and led to supershifts. In contrast, mutants with changes in the amino-terminal cysteine-rich region lost the ability to bind to CREB. The S10A mutant protein bound moderately. Thus, the amino terminus of Tax is essential for Tax-CREB interaction.
Goren
I
I
Max-Planck-Institut fuer Molekulare Genetik, Berlin (Dahlem), Federal Republic of Germany.
Semmes
O J
OJ
Jeang
K T
KT
Moelling
K
K
D000595:Amino Acid Sequence; D001665:Binding Sites; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism*; D003545:Cysteine; D004279:DNA, Viral / Q000378:metabolism; D016356:Gene Products, tax / Q000737:chemistry / Q000378:metabolism*; D015368:Human T-lymphotropic virus 1 / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D011485:Protein Binding; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D015183:Restriction Mapping; D016335:Zinc Fingers
D017362:Cyclic AMP Response Element-Binding Protein; D004279:DNA, Viral; D016356:Gene Products, tax; D011994:Recombinant Proteins; D003545:Cysteine