Introduction of the octanucleotide restriction site SwaI into the bicistronic vector pTiSDT for high level synthesis of proteins.
The octanucleotide recognition site for the endonuclease SwaI was introduced into the Escherichia coli bicistronic expression vector pTiSDT by mutating a single position in the coupling SD sequence between a truncated form of the cro-gene and the multicloning site. This mutation does not influence the expression rate. The introduction of this restriction site allows high level production of proteins, that are modified only by an N-terminal methionine incorporated as the start codon.
Grütz
G
G
Institute of Medical Immunology, Humboldt-University, Medical School Charité, Berlin, Germany.
Randow
F
F
Niemann
B
B
von Baehr
R
R
D001483:Base Sequence; D003001:Cloning, Molecular; D003062:Codon; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*
D003062:Codon; D009841:Oligonucleotides; D011506:Proteins; C074767:endodeoxyribonuclease SwaI; D015252:Deoxyribonucleases, Type II Site-Specific