Non-muscle myosin II heavy chain has a cryptic cell-adhesion domain.
We have discovered a cryptic cell-adhesion domain in non-muscle myosin II heavy chain. A 205 kDa cell-adhesion-promoting polypeptide (p205) was extracted from BHK cells by Nonidet P-40 or Dounce homogenization. Adhesion to p205 was specifically inhibited by the peptide Gly-Arg-Gly-Asp-Ser-Pro, indicating a role for the Arg-Gly-Asp cell-adhesion motif. Purified p205 was identified as non-muscle myosin II heavy chain, based on sequence analysis and on the cross-reactivity of p205 with anti-(bovine trachea myosin) antibodies. Further experiments showed that the heavy chain of purified myosin II has cell-adhesion-promoting activity in a cell-blotting assay, and cross-reacted with anti-p205 antibodies. Finally, the adhesion domain was located in the tail portion of myosin II heavy chain, where an Arg-Gly-Asp-containing sequence can be found.
Grinnell
F
F
Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235, USA.
Ho
C H
CH
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D002448:Cell Adhesion; D002460:Cell Line; D006224:Cricetinae; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000737:chemistry; D009218:Myosins / Q000737:chemistry* / Q000302:isolation & purification
D009218:Myosins