Effects of deferoxamine and a diet deficient in vitamin E on isoelectric electroencephalographic responses associated with ischemia by the four vessel occlusion method.
This study reports the effects of subchronic administration of the iron chelator deferoxamine (4.2 mg/day by osmotic minipump for 6 days) and a diet deficient in Vitamin E (15% RDA for 60 days) on the isoelectric electroencephalographic responses associated with 15 minutes of global transient cerebral ischemia in rats. Brain levels of thiobarbiturate-reacting substance (TBARS), a measure of lipid peroxidation, were lower in deferoxamine-treated animals and higher in Vitamin E deficit animals suggesting the treatments altered free radical activity at the time of ischemia. During ischemia, all test animals were observed to lose the righting reflex and enter a quiescent state. Fifty percent of the animals in two control groups (N = 15 per group) demonstrated an isoelectric electroencephalographic pattern (defined as 10% or less of pre-ischemia total EEG power) with a mean onset of 5.44 minutes. One third of the animals treated with deferoxamine (N = 15) experienced an isoelectric encephalogram with a mean onset of 8.6 minutes and 73% of the Vitamin E-deficient group (N = 15) experienced an isoelectric EEG with a mean onset of 3.43 minutes. Following reperfusion, EEG patterns returned to power levels within 20% of pre-ischemia levels in all animals. Control animals obtained this EEG power level within 1.34 minutes, deferoxamine-treated animals within 1.25 minutes and animals provided a diet deficient in Vitamin E within 5.03 minutes. Compared to mean total EEG power prior to the onset of ischemia, mean total EEG power five days after reperfusion was reduced 14% in the control groups and 59% in the Vitamin E-deficient group and increased 123% in the deferoxamine group. Results are discussed in relation to the possible involvement of free radicals in the ischemic and postischemic process.
Lin
X M
XM
Department of Physiology and Pharmacology, University of South Dakota School of Medicine, Vermillion 57069-2390, USA.
Waller
S B
SB
Dietz
N J
NJ
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology*; D003676:Deferoxamine / Q000494:pharmacology*; D004032:Diet; D004569:Electroencephalography / Q000187:drug effects; D002546:Ischemic Attack, Transient / Q000503:physiopathology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*; D014811:Vitamin E Deficiency / Q000503:physiopathology*
D017392:Thiobarbituric Acid Reactive Substances; D003676:Deferoxamine