Early detection of amphotericin B induced nephrotoxicity by 99mTc-DTPA: a useful test.
Amphotericin B (AMB) with a broad spectrum of antifungal activity is used for the treatment of life-threatening mycoses, especially in immunocomprised patients. Since measurements of the blood level of AMB and of creatinine do not provide early warning of AMB induced renal toxicity, we studied the effects of AMB on biodistribution of a glomerular agent, 99mTc-DTPA. In Swiss mice, the toxicity of AMB was studied at single intravenous doses of 0.5-3.5 mg/kg body weight. Dose dependent effects consisted of decreased blood clearance and urinary excretion. Recovery of function was shown in dose ranges corresponding to high level clinical schedules. Serum creatinine changes lagged behind the 99mTc-DTPA alterations. This suggests that AMB toxicity might be monitored by blood/urinary clearance of 99mTc-DTPA.
Sawas-Dimopoulou
C
C
Institute of Radioisotopes and Radiodiagnostic Products, National Center for Scientific Research Demokritos, Aghia Paraskevi Attikis, Greece.
Papathanassiou
P
P
Margaritis
L
L
D000666:Amphotericin B / Q000633:toxicity*; D000818:Animals; D003404:Creatinine / Q000097:blood; D004305:Dose-Response Relationship, Drug; D007275:Injections, Intravenous; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008657:Metabolic Clearance Rate; D051379:Mice; D016284:Technetium Tc 99m Pentetate / Q000493:pharmacokinetics*; D014018:Tissue Distribution
D000666:Amphotericin B; D003404:Creatinine; D016284:Technetium Tc 99m Pentetate