title: The detection of clonal proliferation in granular lymphocyte-proliferative disorders of natural killer cell lineage.
abstract: The clonal proliferation of large granular lymphocytes can be detected in patients with T-cell-lineage granular lymphocyte-proliferative disorders (T-GLPD) by Southern blotting T-cell receptor genes. However, this cannot be applied to patients with natural killer-cell-lineage GLPD (NK-GLPD) as it lacks a clonal marker. We therefore investigated the use of two other diagnostic techniques in evaluating clonal proliferation in Japanese patients with NK-GLPD (n = 4) and T-GLPD (n = 3) by chromosomal analysis of peripheral blood mononuclear cells (PBMC) stimulated with either interleukin-2 or phytohaemagglutinin, and Epstein-Barr viral (EBV) genomic DNA analysis. Chromosomal analysis revealed abnormal karyotypes in the PBMC of three of four patients with NK-GLPD, whereas EBV analysis showed a monoclonal terminal configuration in the PBMC in the fourth patient. Southern blots revealed rearrangements of the TCR genes in all three patients with T-GLPD but in none of those with NK-GLPD. It is suggested that these methods may be useful in detecting the abnormal proliferation of large granular lymphocytes in NK-GLPD.
authors.affiliation: Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Shimodaira
authors.forename: F
authors.initials: F
authors.lastname: Ishida
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: B
authors.initials: B
authors.lastname: Mahbub
authors.forename: K
authors.initials: K
authors.lastname: Kawa-Ha
authors.forename: K
authors.initials: K
authors.lastname: Kitano
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D015139:Blotting, Southern; D002455:Cell Division; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D015329:Gene Rearrangement, T-Lymphocyte; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D007621:Karyotyping; D007694:Killer Cells, Natural / Q000473:pathology*; D008232:Lymphoproliferative Disorders / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D013601:T-Lymphocytes / Q000473:pathology
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D004279:DNA, Viral