Non-isotopic in situ hybridization at the ultrastructural level.
Non-isotopic in situ hybridization techniques are becoming increasingly widely used at the ultrastructural level, permitting rapid localization of nucleic acid targets with a high degree of resolution. Technical considerations dictate that the great specificity of the method cannot be matched by a similar degree of sensitivity; the value of non-isotopic ultrastructural in situ hybridization lies in its unique ability to localize nucleic acid targets in relation to submicroscopic cellular structures. This article presents an overview of non-isotopic ultrastructural hybridization methods and applications.
Morey
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Division of Anatomical Pathology, St Vincent's Hospital, Sydney, N.S.W., Australia.
D000818:Animals; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods*; D008854:Microscopy, Electron; D009696:Nucleic Acids / Q000032:analysis*
D009696:Nucleic Acids