title: Engraftment kinetics and long-term stability of hematopoiesis following autografting of peripheral blood stem cells.
abstract: BACKGROUND
  We analyzed short-term and sustained hematopoietic reconstitution after high-dose therapy with peripheral blood stem cell (PBSC) support in patients with various malignant disorders.
  
  
  METHODS
  Fifty-six patients, all with malignant hematologic disorders, were autografted between 1989 and 1994 using PBSC (47 pts) or PBSC + bone marrow (BM) cells (9 pts). PBSC were collected after mobilization with chemotherapy +/- hematopoietic growth factors (GF).
  
  
  RESULTS
  All patients engrafted > 0.5 x 10(9)/L polymorphonuclear cells (PMN) and > 50.0 x 10(9)/L Plt at a median of 12 (8-32) and 13 (9-365) days, respectively. Thirty-nine patients were evaluable for long-term graft performance, and their hematologic values at 30 and 100 days, at 6 months and at 1, 2, 3, 4 and 5 years were retrospectively analyzed. Steady counts were recorded over the years. None of the patients had late graft failure.
  
  
  CONCLUSIONS
  PBSC given after high-dose chemotherapy ensure a fast hematologic recovery with stable graft performance up to five years after autograft. Though this is not definitive proof of the presence of uncommitted stem cells in the PBSC population, it gives further support to the idea that PBSC are as safe as bone marrow for long-term engraftment. A delayed or incomplete recovery of platelets may occur with low PBSC counts or when disease relapse occurs rapidly after autograft.
authors.affiliation: Department of Hematology, Ospedale Cervello, Palermo, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Indovina
authors.forename: I
authors.initials: I
authors.lastname: Majolino
authors.forename: F
authors.initials: F
authors.lastname: Buscemi
authors.forename: R
authors.initials: R
authors.lastname: Scimè
authors.forename: S
authors.initials: S
authors.lastname: Vasta
authors.forename: A
authors.initials: A
authors.lastname: Santoro
authors.forename: M
authors.initials: M
authors.lastname: Pampinella
authors.forename: P
authors.initials: P
authors.lastname: Catania
authors.forename: F
authors.initials: F
authors.lastname: Caronia
mesh_terms: D000293:Adolescent; D000328:Adult; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000009:adverse effects / Q000627:therapeutic use; D001855:Bone Marrow Diseases / Q000139:chemically induced / Q000628:therapy; D016026:Bone Marrow Transplantation; D002648:Child; D005260:Female; D006085:Graft Survival; D006410:Hematopoiesis; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009369:Neoplasms / Q000188:drug therapy / Q000628:therapy*; D012189:Retrospective Studies