title: Relationship between phosphoinositide hydrolysis and prostaglandin F2 alpha secretion in vitro from endometrium of cyclic pigs on day 15 postestrus.
abstract: The mechanism for the luteolytic release of prostaglandin (PG)F2 alpha in swine is not known. This study examined the potential role of oxytocin (OT)-induced phosphoinositide (PI) hydrolysis in promoting PGF2 alpha secretion in vitro from the endometrium of cyclic gilts on Day 15 postestrus. In Experiment 1, endometrial PI hydrolysis was increased (P < 0.05) by 100 nM OT and was increased quadratically (P < 0.05) by LiCl, but was not affected by the LiCl x OT interaction (P > 0.30). PI hydrolysis was maximal at 50 mM LiCl and declined at 100 mM LiCl. In Experiment 2, endometrial PI hydrolysis and PGF2 alpha secretion were similarly increased (P < 0.01) by 0, 0.1, 1, 10, and 100 nM OT in a dose-dependent manner. In Experiment 3, the linear increase in PI hydrolysis occurring 0, 3, 5, 10, and 20 min after treatment was greater (P = 0.01) for tissue treated with 100 nM OT than for the tissue treated with 0 nM OT. The quadratic increase (P < 0.05) in PGF2 alpha secretion occurring 0, 3, 5, 10, and 20 min after treatment was greater (P < 0.05) for tissue treated with 100 nM OT than for the tissue treated with 0 nM OT. In Experiment 4, AlF4- (an activator of Gp and phospholipase C) similarly increased (P < 0.01) PI hydrolysis and PGF2 alpha secretion. In Experiment 5, PI hydrolysis (P < 0.01) and PGF2 alpha secretion (P < 0.05) were increased by 100 nM OT but were not stimulated by cholera toxin (an activator of Gs and adenylate cyclase). Cholera toxin also did not enhance PI hydrolysis and PGF2 alpha secretion in response to 0.1 or 100 nM OT. These results are consistent with the hypothesis that OT may induce PI hydrolysis to stimulate the endometrial secretion of PGF2 alpha during corpus luteum regression in swine.
authors.affiliation: Department of Animal Sciences, Washington State University, Pullman 99164-6332, USA.
authors.forename: S S
authors.initials: SS
authors.lastname: Whiteaker
authors.forename: M A
authors.initials: MA
authors.lastname: Mirando
authors.forename: W C
authors.initials: WC
authors.lastname: Becker
authors.forename: D N
authors.initials: DN
authors.lastname: Peters
mesh_terms: D000375:Aging / Q000378:metabolism / Q000502:physiology; D000818:Animals; D046508:Culture Techniques; D015237:Dinoprost / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004717:Endometrium / Q000378:metabolism*; D004971:Estrus / Q000378:metabolism* / Q000502:physiology; D005260:Female; D006868:Hydrolysis; D018021:Lithium Chloride / Q000494:pharmacology; D003341:Luteolysis / Q000378:metabolism / Q000502:physiology; D010121:Oxytocin / Q000494:pharmacology; D010716:Phosphatidylinositols / Q000378:metabolism*; D013552:Swine / Q000378:metabolism*; D013997:Time Factors
chemical_list: D010716:Phosphatidylinositols; D010121:Oxytocin; D015237:Dinoprost; D018021:Lithium Chloride