Improved method for polyamine determination in TMV, a rod-shaped virus.
Polyamines were measured in viruses using different techniques. An improved method of polyamine analysis is reported for tobacco mosaic virus (TMV), a rod-shaped virus (95% protein and 5% RNA), based on HPLC of sonicated PCA-treated highly purified virus suspensions. This method allowed higher and more reliable recovery of TMV polyamines (putrescine, spermidine and spermine) when compared to the HPLC of non-sonicated samples and to thin layer chromatography. It is suggested that sonication acts on PCA-precipitated protein aggregates causing the release of trapped polyamine molecules.
Torrigiani
P
P
Dipartimento di Biologia evoluzionistica sperimentale, University of Bologna, Italy.
Rabiti
A L
AL
Betti
L
L
Marani
F
F
Brizzi
M
M
Bagni
N
N
Canova
A
A
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010472:Perchlorates / Q000494:pharmacology; D011073:Polyamines / Q000032:analysis*; D011700:Putrescine / Q000032:analysis; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis; D014027:Tobacco Mosaic Virus / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure
D010472:Perchlorates; D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine