title: Kinetic investigation of the action of hyaluronidase on hyaluronan using the Morgan-Elson and neocuproine assays.
abstract: In this paper we describe kinetic investigations of the action of testicular hyaluronidase on hyaluronan. We have compared the use of two spectrophotometric assays, the first based on the Morgan-Elson reaction and the second on the neocuproine reaction. With the neocuproine reaction Km was found to be 0.46 mg/ml and Vmax to be 126 nmol l-1 s-1. Because of a low sensitivity and the production of interfering precipitates, the Morgan-Elson assay cannot be used for kinetic investigation of the enzyme. Furthermore this assay is prone to interference from compounds such as disodium cromoglycate, (+)-catechine, penicillamine, CaCl2 and acetate buffer.
authors.affiliation: Laboratory of General Biochemistry, University of Ghent, Belgium.
authors.forename: K P
authors.initials: KP
authors.lastname: Vercruysse
authors.forename: A R
authors.initials: AR
authors.lastname: Lauwers
authors.forename: J M
authors.initials: JM
authors.lastname: Demeester
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism; D002240:Carbohydrate Sequence; D002614:Chelating Agents / Q000737:chemistry; D006820:Hyaluronic Acid / Q000378:metabolism*; D006821:Hyaluronoglucosaminidase / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D010618:Phenanthrolines / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D002614:Chelating Agents; D010618:Phenanthrolines; C002701:neocuproine; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase; D000117:Acetylglucosamine