title: Antibodies to postsynaptic PKC substrate neurogranin prevent long-term potentiation in hippocampal CA1 neurons.
abstract: Both pre- and postsynaptic protein kinase C have been implicated in long-term potentiation. Neurogranin (also known as BICKs and RC3) is a neuronal postsynaptic protein kinase C substrate. In the present study we injected monoclonal IgGs that recognize the protein kinase C phosphorylation site in neurogranin and B-50 (GAP-43), and that have been shown to inhibit protein kinase C-mediated B-50 phosphorylation, through a whole-cell clamp pipette into CA1 pyramidal neurons in rat hippocampal slices. Injection of neurogranin IgGs, but not of control IgGs, prevented the induction of tetanus-induced long-term potentiation without affecting post-tetanic potentiation. Our results suggest that neurogranin is involved in mechanisms of activity-dependent synaptic plasticity.
authors.affiliation: Department of Neurophysiology, Institute for Neurobiology, Magdeburg, Germany.
authors.forename: N B
authors.initials: NB
authors.lastname: Fedorov
authors.forename: P
authors.initials: P
authors.lastname: Pasinelli
authors.forename: A B
authors.initials: AB
authors.lastname: Oestreicher
authors.forename: P N
authors.initials: PN
authors.lastname: DeGraan
authors.forename: K G
authors.initials: KG
authors.lastname: Reymann
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002148:Calmodulin-Binding Proteins / Q000276:immunology / Q000378:metabolism*; D005071:Evoked Potentials / Q000502:physiology; D019922:GAP-43 Protein; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D007074:Immunoglobulin G / Q000502:physiology; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects*; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000378:metabolism; D051579:Neurogranin; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011493:Protein Kinase C / Q000378:metabolism*; D017966:Pyramidal Cells / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D013569:Synapses / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D002148:Calmodulin-Binding Proteins; D019922:GAP-43 Protein; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; C496651:Nrgn protein, rat; D051579:Neurogranin; D011493:Protein Kinase C