title: Effect of purinergic stimulation on intracellular calcium concentration and transepithelial potential difference in cultured bovine oviduct cells.
abstract: Epithelial cells were removed from bovine oviducts by enzyme digestion and either cultured on laminin-coated coverslips (for determination of [Ca2+]i) or on collagen filters (for determination of transepithelial potential difference [pd]). Cells on coverslips were loaded with Fura-2 to monitor [Ca2+]i. Application of extracellular ATP induced a transient increase in [Ca2+]i in a dose-dependent manner. This response was abolished by thapsigargin, indicating that the rise in [Ca2+]i was derived from intracellular stores. The order of potency of the nucleotide-induced rise in [Ca2+]i was uridine triphosphate (UTP)>ATP>ADP. Epithelial cells were grown on collagen filters, and when mounted in a modified Ussing chamber exhibited an electrical pd of 1.00 +/- 0.36 mV with the apical side negative with respect to the basal. Application of UTP, ATP, and ADP to the basal side induced transient increases in pd of 1.15 +/- 0.21, 0.77 +/- 0.16, and 0.26 +/- 0.06 mV, respectively. The order of potency of the nucleotides in eliciting transient increases in [Ca2+]i and pd suggests the presence of a P2u purinergic receptor in the bovine oviduct epithelium that could play a role in transepithelial ion movements and hence the control of oviductal fluid formation.
authors.affiliation: Department of Biology, University of York, Heslington, United Kingdom.
authors.forename: C I
authors.initials: CI
authors.lastname: Cox
authors.forename: H J
authors.initials: HJ
authors.lastname: Leese
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D005187:Fallopian Tubes / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005455:Fluorescent Antibody Technique; D008564:Membrane Potentials; D011687:Purines / Q000494:pharmacology*; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin; D014544:Uridine Triphosphate / Q000494:pharmacology
chemical_list: D011687:Purines; D013729:Terpenes; D000244:Adenosine Diphosphate; D019284:Thapsigargin; D000255:Adenosine Triphosphate; D002118:Calcium; D014544:Uridine Triphosphate