title: Two independent pathways of expression lead to self-assembly of the rabbit hemorrhagic disease virus capsid protein.
abstract: The rabbit hemorrhagic disease virus capsid protein was expressed in insect cells either as an individual protein species, from a mRNA analogous to the viral subgenomic RNA, or as part of a polyprotein that included the viral 3C-like protease and the RNA polymerase. Both pathways of expression led to the assembly of viruslike particles morphologically and antigenically similar to purified virus.
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell' Emilia, Brescia, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Sibilia
authors.forename: M B
authors.initials: MB
authors.lastname: Boniotti
authors.forename: P
authors.initials: P
authors.lastname: Angoscini
authors.forename: L
authors.initials: L
authors.lastname: Capucci
authors.forename: C
authors.initials: C
authors.lastname: Rossi
mesh_terms: D000818:Animals; D016367:Baculoviridae; D001483:Base Sequence; D002213:Capsid / Q000032:analysis / Q000096:biosynthesis*; D002460:Cell Line; D017931:DNA Primers; D015342:DNA Probes; D015870:Gene Expression; D005822:Genetic Vectors; D017929:Hemorrhagic Disease Virus, Rabbit / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D007313:Insecta; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis
chemical_list: D017931:DNA Primers; D015342:DNA Probes; D011994:Recombinant Proteins