title: The Agrobacterium tumefaciens virulence D2 protein is responsible for precise integration of T-DNA into the plant genome.
abstract: The VirD2 protein of Agrobacterium tumefaciens was shown to pilot T-DNA during its transfer to the plant cell nucleus. We analyze here its participation in the integration of T-DNA by using a virD2 mutant. This mutation reduces the efficiency of T-DNA transfer, but the efficiency of integration of T-DNA per se is unaffected. Southern and sequence analyses of integration events obtained with the mutated VirD2 protein revealed an aberrant pattern of integration. These results indicate that the wild-type VirD2 protein participates in ligation of the 5'-end of the T-strand to plant DNA and that this ligation step is not rate limiting for T-DNA integration.
authors.affiliation: Friedrich Miescher-Institut, Basel, Switzerland.
authors.forename: B
authors.initials: B
authors.lastname: Tinland
authors.forename: F
authors.initials: F
authors.lastname: Schoumacher
authors.forename: V
authors.initials: V
authors.lastname: Gloeckler
authors.forename: A M
authors.initials: AM
authors.lastname: Bravo-Angel
authors.forename: B
authors.initials: B
authors.lastname: Hohn
mesh_terms: D016960:Agrobacterium tumefaciens / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics; D004269:DNA, Bacterial / Q000378:metabolism*; D018745:Genome, Plant; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D010947:Plants, Toxic; D014026:Nicotiana / Q000235:genetics*; D037521:Virulence Factors
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C036483:T-DNA; D037521:Virulence Factors