[Features of the structure of the 7K-copy of Drosophila MDG4 (gypsy) retrotransposon provides evidence that the 7K-subfamily of MDG4 is potentially capable of transposition].
The complete nucleotide sequence of the 7K variant of the gypsy retrotransposon of Drosophila melanogaster was determined. This variant belongs to the 7K subfamily of gypsy, which was previously considered inactive. All differences found in the sequenced 7K copy compared to the transpositionally active 6K variants were point mutations. These nucleotide substitutions account for about 1% of the total base pair number of gypsy. Long terminal repeats (LTR) have the highest rate of nucleotide substitutions However, changes in nontranslated regions did not involve the promoter region and other supposed cis-acting elements. Sixteen amino acid substitutions were found in the coding region of gypsy. These substitutions were mainly located on the boarders of potential functional domains and within the third open reading frame (ORF3). Comparative analysis of structures of these two variants of gypsy suggests the potential ability of 7K copies to transpose.
Avedisov
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Il'in
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D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004330:Drosophila / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D017354:Point Mutation; D012091:Repetitive Sequences, Nucleic Acid; D018626:Retroelements
D018626:Retroelements; D004247:DNA
Osobennosti struktury 7K-kopiĭ retrotranspozona drozofily MDG4 (gypsy)svidetel'stvuiut o potentsial'noĭ sposobnosti 7K-podsemeĭstva MDG4 k transpozitsiiam.