Molecular characterization of cDNA encoding a novel protein related to transcriptional enhancer factor-1 from neural precursor cells.
We identified a novel cDNA related to that of transcriptional enhancer factor-1 (TEF-1) during the course of isolation and characterization of cDNAs, whose mRNAs are preferentially expressed in the mouse neural precursor cells. The putative polypeptide, termed embryonic TEA domain-containing factor (ETF), deduced from the nucleotide sequence contains 445 amino acids and shares 66% amino acid identity with mouse and human TEF-1 proteins. The primary structure of the TEA domain, a probable DNA-binding domain, and the specific DNA binding activity to the GT-IIC motif of ETF are indistinguishable from those of the known vertebrate TEF-1 proteins. However, the expression of the ETF gene is strictly regulated in developing embryos and is limited to certain tissues, such as the hindbrain of a 10-day-old mouse embryo, in contrast to the ubiquitous expression pattern of the TEF-1 gene. These results suggest that ETF is a novel mammalian member of the TEA domain-containing transcription factor family and may be involved in the gene regulation of the neural development. We have discussed the possible existence of multiple subtypes of the mammalian TEF-1 family proteins, which may play different roles in cellular and development gene regulation.
Yasunami
M
M
Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Japan.
Suzuki
K
K
Houtani
T
T
Sugimoto
T
T
Ohkubo
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D009420:Nervous System / Q000737:chemistry / Q000196:embryology* / Q000254:growth & development; D009687:Nuclear Proteins; D011485:Protein Binding; D012333:RNA, Messenger / Q000096:biosynthesis; D012249:Rhombencephalon / Q000737:chemistry / Q000196:embryology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013234:Stem Cells / Q000737:chemistry*; D000091262:TEA Domain Transcription Factors; D013997:Time Factors; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D000091262:TEA Domain Transcription Factors; C057182:TEAD1 protein, human; C486310:Tead2 protein, mouse; D014157:Transcription Factors