Octopamine mediates rapid stimulation of protein kinase A in the antennal lobe of honeybees.
In the honeybee octopamine mediates mechanisms of arousal that interfere with the appetitive proboscis extension response to food-indicating chemosensory stimuli. This study demonstrates that injections of octopamine or cyclic adenosine monophosphate (cAMP) into the primary chemosensory neuropil of the honeybee, the antennal lobe, evokes a rapid and transient activation of cAMP-dependent protein kinase (PKA). Other monoamines detectable in the antennal lobe, dopamine and serotonin, do not affect the level of PKA activity. Stimulation of the bees' antenna with the appetitive stimulus water or sucrose solution in vivo also causes a short-term activation of PKA in the antennal lobe. The increased PKA activity can be detected immediately (0.5 s) after stimulation but reverts to the basal level within 3 s. This effect can be abolished by monoamine depletion with reserpine. Since octopamine is the only monoamine that stimulates PKA, it appears to mediate the PKA activation after sucrose stimulus and may contribute to the processing of this chemosensory input.
Hildebrandt
H
H
Institut für Neurobiologie, Freien Universität Berlin, Germany.
Müller
U
U
D000818:Animals; D001516:Bees / Q000187:drug effects* / Q000201:enzymology; D015306:Biogenic Monoamines / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D017868:Cyclic AMP-Dependent Protein Kinases / Q000187:drug effects*; D004789:Enzyme Activation; D009655:Octopamine / Q000502:physiology*; D011897:Random Allocation; D011984:Sensory Receptor Cells / Q000187:drug effects* / Q000201:enzymology; D013395:Sucrose / Q000494:pharmacology; D013997:Time Factors
D015306:Biogenic Monoamines; D009655:Octopamine; D013395:Sucrose; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases