[Development of the monoclonal antibody to cultured uterine endometrial adenocarcinoma cells (EI)].
A new monoclonal antibody (Moab), 3H2, was produced by immunizing Balb/C mouse with EI, a newly established human cell line from moderately differentiated endometrial adenocarcinoma of the uterus. The molecular weight of the Moab 3H2-defined antigen was 36,000 daltons, and suggests that the epitope of the Moab 3H2-defined antigen was protein moiety. Moab 3H2 was of the IgG1, subclass. Using formalin-fixed, paraffin-embedded human tissues and immunohistochemical techniques (ABC method), Moab 3H2 has demonstrated reactivity, Moab 3H2 reacted with well and moderately differentiated endometrial adenocarcinoma of the uterus, but did not react with poorly differentiated endometrial adenocarcinoma of the uterus, Moab 3H2 did not react with other tissues, malignant or normal, except ovarian clear cell adenocarcinoma, suggesting that Moab 3H2 reacts with well and moderately differentiated endometrial adenocarcinoma of the uterus with high specificity. To analyze the epitope of the Moab 3H2-defined antigen, it should be useful to make clear the function of carcinogenesis, differentiation and growth of endometrial adenocarcinoma of the uterus.
Yudate
T
T
Department of Obstetrics and Gynecology, Tokyo Medical College.
Isaka
K
K
Okabe
K
K
Takayama
M
M
D000230:Adenocarcinoma / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000032:analysis*; D002459:Cell Fusion; D016889:Endometrial Neoplasms / Q000276:immunology*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm