title: A mutated intron sequence codes for an antigenic peptide recognized by cytolytic T lymphocytes on a human melanoma.
abstract: We have identified an antigen recognized on a human melanoma by autologous cytolytic T lymphocytes. It is encoded by a gene that is expressed in many normal tissues. Remarkably, the sequence coding for the antigenic peptide is located across an exon-intron junction. A point mutation is present in the intron that generates an amino acid change that is essential for the recognition of the peptide by the anti-tumor cytotoxic T lymphocytes. This observation suggests that the T-cell-mediated surveillance of the integrity of the genome may extend to some intronic regions.
authors.affiliation: Ludwig Institute for Cancer Research, UCL 7459, Brussels, Belgium.
authors.forename: P G
authors.initials: PG
authors.lastname: Coulie
authors.forename: F
authors.initials: F
authors.lastname: Lehmann
authors.forename: B
authors.initials: B
authors.lastname: Lethé
authors.forename: J
authors.initials: J
authors.lastname: Herman
authors.forename: C
authors.initials: C
authors.lastname: Lurquin
authors.forename: M
authors.initials: M
authors.lastname: Andrawiss
authors.forename: T
authors.initials: T
authors.lastname: Boon
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D001667:Binding, Competitive; D002460:Cell Line; D003001:Cloning, Molecular; D003602:Cytotoxicity, Immunologic; D005091:Exons; D006801:Humans; D007438:Introns; D007700:Kinetics; D008545:Melanoma / Q000276:immunology*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D017354:Point Mutation; D011994:Recombinant Proteins / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins; D011994:Recombinant Proteins