Similarities of an autoantigen in aneurysmal disease of the human abdominal aorta to a 36-kDa microfibril-associated bovine aortic glycoprotein.
Immunoglobulin G (IgG) from human aneurysmal aorta was used to partially purify an aortic protein with an apparent MW approximately 80 kDa. Amino acid sequencing of a tryptic digest revealed two sequences with homology to mouse tenascin-X. The autoimmune IgG was then shown to react with purified human tenascin, and a rabbit polyclonal anti-human tenascin antibody was found to react with the purified autoantigen. These observations suggest that the autoantigen of abdominal aortic aneurysm disease may be homologous to a calcium-binding member of the tenascin superfamily that has been identified by others in pig and cow.
Tilson
M D
MD
Department of Surgery, St. Luke's/Roosevelt Hospital Center, Columbia University, New York, NY 10583, USA.
D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000737:chemistry / Q000378:metabolism*; D017544:Aortic Aneurysm, Abdominal / Q000276:immunology*; D001324:Autoantigens / Q000737:chemistry* / Q000302:isolation & purification; D015153:Blotting, Western; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D003285:Contractile Proteins / Q000737:chemistry* / Q000302:isolation & purification; D004547:Elastic Tissue; D016326:Extracellular Matrix Proteins / Q000737:chemistry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry* / Q000302:isolation & purification; D013058:Mass Spectrometry; D051379:Mice; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009131:Muscle, Smooth, Vascular / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D000072260:RNA Splicing Factors; D017386:Sequence Homology, Amino Acid; D019063:Tenascin; D014357:Trypsin
D001324:Autoantigens; D003285:Contractile Proteins; D016326:Extracellular Matrix Proteins; D007074:Immunoglobulin G; D010446:Peptide Fragments; D000072260:RNA Splicing Factors; D019063:Tenascin; C016598:microfibrillar protein; C081865:tenascin X; D014357:Trypsin