Diagnosis of congenital toxoplasmosis by immunoblotting and relationship with other methods.
Immunoblot has been evaluated as a diagnostic method for congenital toxoplasmosis. Like enzyme-linked immunofiltration assay (ELIFA), immunoblot can be used to compare antibody patterns and to determine if the antibodies are transmitted by the mother or synthesized by the fetus or infant. Among the 48 infants tested, 27 had congenital toxoplasmosis and 21 were suspected but had none. Reproducibility, sensitivity, specificity, and positive predictive values in immunoblot for immunoglobulins (Igs) G+M+A and/or G+M were 90, 92.6, 89.1, and 92.4%, respectively. G+M immunoblot and G+M ELIFA have better sensitivities than the conventional IgM immunosorbent agglutination assay, IgM enzyme-linked immunosorbent assay (ELISA), IgM immunofluorescence antibody test, in vitro culture, and mouse inoculation. The novel antibodies, i.e., those synthesized by infants against Toxoplasma gondii, were of the IgG class in most cases, although a confident diagnosis could be related to the number of observed Ig classes (G+M, G+A, and G+M+A). Immunoblot has a better resolution than ELIFA. In prenatal diagnosis, immunoblot could be complementary to in vitro culture and mouse inoculation. In the other cases, early detection by immunoblot appears to give the best results when compared with the other serological methods.
Chumpitazi
B F
BF
Département de Parasitologie Mycologie Médicale et Moléculaire, Université Joseph Fourier-Grenoble I, Centre National de la Recherche Scientifique, La Tronche, France.
Boussaid
A
A
Pelloux
H
H
Racinet
C
C
Bost
M
M
Goullier-Fleuret
A
A
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D015415:Biomarkers / Q000097:blood; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D015151:Immunoblotting / Q000379:methods* / Q000706:statistics & numerical data; D007231:Infant, Newborn; D007770:L-Lactate Dehydrogenase / Q000097:blood; D051379:Mice; D011247:Pregnancy; D011296:Prenatal Diagnosis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors; D014122:Toxoplasma / Q000276:immunology; D014125:Toxoplasmosis, Congenital / Q000175:diagnosis* / Q000276:immunology; D011503:Transglutaminases / Q000378:metabolism
D000913:Antibodies, Protozoan; D015415:Biomarkers; D007770:L-Lactate Dehydrogenase; D011503:Transglutaminases