title: Transfection of Sendai virus F gene cDNA with mutations at its cleavage site and HN gene cDNA into COS cells induces cell fusion.
abstract: In contrast to the wild type Sendai virus fusion protein (F), a mutated F to possess a cleavage site similar to that of virulent Newcastle disease virus F, could be cleaved by proteases present in COS cells. When mutated F and hemagglutinin-neuraminidase (HN) were coexpressed at the cell surface, syncytium formation was observed.
authors.affiliation: Department of Bioscience and Technology, Faculty of Agriculture, Iwate University, Morioka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Taira
authors.forename: T
authors.initials: T
authors.lastname: Sato
authors.forename: H
authors.initials: H
authors.lastname: Segawa
authors.forename: M
authors.initials: M
authors.lastname: Chiba
authors.forename: T
authors.initials: T
authors.lastname: Katsumata
authors.forename: K
authors.initials: K
authors.lastname: Iwasaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral; D015726:Giant Cells / Q000821:virology; D015707:HN Protein / Q000235:genetics / Q000502:physiology*; D006868:Hydrolysis; D008561:Membrane Fusion; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010222:Parainfluenza Virus 1, Human / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014760:Viral Fusion Proteins / Q000235:genetics / Q000502:physiology*
chemical_list: D004279:DNA, Viral; D015707:HN Protein; D014760:Viral Fusion Proteins