Molecular cloning and sequence analysis of human preprocathepsin C.
A cDNA clone (C1) coding for human preprocathepsin C was isolated from a human ileum cDNA library using a rat kidney-derived RT-PCR probe and its complete nucleotide sequence determined. The full-length 1857 bp sequence codes for a protein of 463 amino acid residues with a calculated molecular mass of 51848 Da. Comparison of the deduced amino acid sequence with that of rat preprocathepsin C indicates an 87.5% identity. A multiple alignment of the deduced cathepsin C sequence of 233 residues which, by analogy to other cystein proteinases, corresponds to the mature protein, confirms that human cathepsin C belongs to the papain superfamily.
Paris
A
A
Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, Ljubljana, Slovenia.
Strukelj
B
B
Pungercar
J
J
Renko
M
M
Dolenc
I
I
Turk
V
V
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002403:Cathepsins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004792:Enzyme Precursors / Q000737:chemistry / Q000235:genetics*; D018628:Gene Dosage; D005796:Genes / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010206:Papain / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D004792:Enzyme Precursors; D002403:Cathepsins; C095231:preprocathepsin C; D010206:Papain