title: Erythrocyte-mediated toxification of 1,8-dinitropyrene. II. Covalent bind to erythrocyte-proteins.
abstract: In the present study we have shown that erythrocyte-catalyzed 1,8-dinitropyrene reduction occurs via formation of reactive intermediate species, which bind covalently to haemoglobin and other erythrocyte proteins. In fact after mild-acid hydrolysis of lysate proteins exposed to DNP, the reduced metabolites, 1-amino-8-nitropyrene and 1,8-diaminopyrene, were released, thus indicating the formation of sulphinamide adducts to proteins. These results suggest that haemoglobin adduct biomonitoring would be a useful method of controlling exposure to nitroarene in persons at risk.
authors.affiliation: Dipartimento di Biochimica e Biotechnologie Mediche, Università di Napoli Federico II.
authors.forename: M A
authors.initials: MA
authors.lastname: Belisario
authors.forename: A
authors.initials: A
authors.lastname: Garofalo
authors.forename: M
authors.initials: M
authors.lastname: Maturo
authors.forename: G
authors.initials: G
authors.lastname: Avagnale
authors.forename: N
authors.initials: N
authors.lastname: Sannolo
authors.forename: A
authors.initials: A
authors.lastname: Malorni
mesh_terms: D000588:Amines / Q000032:analysis; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D011721:Pyrenes / Q000378:metabolism*
chemical_list: D000588:Amines; D011506:Proteins; D011721:Pyrenes; C033310:1,8-dinitropyrene