Detection of beet necrotic yellow vein virus strains, variants and mixed infections by examining single-strand conformation polymorphisms of immunocapture RT-PCR products.
Single-strand conformation polymorphism analysis was found to be a powerful tool for rapidly assigning large numbers of beet necrotic yellow vein virus (BNYVV) isolates to a known strain group as well as for detecting mixed infections, minor variants or new strain groups. The prevalence of the B-type in Germany and France and the A-type in most other countries was confirmed. Minor variants with a very restricted distribution were detected occasionally. New rhizomania outbreaks in Great Britain were caused either by the A- or B-type or mixtures of both suggesting introduction of BNYVV from several sites abroad. An entirely different BNYVV type (P-type) was identified in a small area in France. Evidence for further strain groups in China was also obtained.
Koenig
R
R
Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Biochemie und Pflanzenvirologie, Braunschweig, Germany.
Lüddecke
P
P
Haeberlé
A M
AM
D005060:Europe; D007163:Immunosorbent Techniques; D010942:Plant Viruses / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D018807:Polymorphism, Single-Stranded Conformational; D012328:RNA Viruses / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics*; D014675:Vegetables / Q000821:virology
D012367:RNA, Viral