title: Fertilization and pregnancy achieved by intracytoplasmic injection of sperm retrieved from testicular biopsies.
abstract: OBJECTIVE
  To determine if intracytoplasmic sperm injection using testicular sperm is effective in the treatment of infertile couples with obstructive azoospermia.
  
  
  METHODS
  Case report.
  
  
  METHODS
  Tertiary care teaching medical center.
  
  
  METHODS
  Intracytoplasmic sperm injection was performed in two patients using testicular sperm after microsurgical epididymal sperm aspiration failed.
  
  
  RESULTS
  In both cases fertilization and embryos resulted. One patient conceived and delivered triplets. There was no evidence of chromosomal abnormality or congenital malformation.
  
  
  CONCLUSIONS
  Intracytoplasmic sperm injection using sperm extracted from testicular biopsy appears to be effective in the treatment of infertile couples with obstructive azoospermia when microsurgical sperm aspiration fails.
authors.affiliation: Department of Obstetrics and Gynecology, Hurley Medical Center, Flint, Michigan 48503, USA.
authors.forename: M I
authors.initials: MI
authors.lastname: Abuzeid
authors.forename: Y M
authors.initials: YM
authors.lastname: Chan
authors.forename: M A
authors.initials: MA
authors.lastname: Sasy
authors.forename: S
authors.initials: S
authors.lastname: Basata
authors.forename: M
authors.initials: M
authors.lastname: Beer
mesh_terms: D000328:Adult; D001706:Biopsy; D003593:Cytoplasm; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D008845:Microinjections; D009865:Oocytes / Q000648:ultrastructure*; D011247:Pregnancy; D011256:Pregnancy Outcome; D013094:Spermatozoa / Q000502:physiology; D013737:Testis / Q000166:cytology*; D014659:Vasectomy