title: Follicle-stimulating hormone transiently induces expression of protooncogene c-myc in primary Sertoli cell cultures of early pubertal and prepubertal rat.
abstract: The protooncogene c-myc plays an important role in the regulation of cellular proliferation and differentiation. To evaluate the possibility that the protooncogene c-myc plays some roles in follicle-stimulating hormone (FSH)-dependent gene regulation of Sertoli cells, the effects of FSH on the expression of c-myc has been investigated in primary Sertoli cell cultures. FSH was no change to the c-myc mRNA level before 18 h, but transiently increased c-myc mRNA levels, with maximal stimulation reached in 18 h. The induction of c-myc was dependent on the concentration of FSH. Th c-myc mRNA was also increased after treatment with dibutyryl c-AMP and forskolin in primary Sertoli cell cultures. FSH-dependent c-myc mRNA levels were superinduced in cells treated for 3 h with cycloheximide but it was reduced by actinomycin-D pretreatment. Even in the absence of FSH in culture medium c-myc mRNA was clearly detectable in Sertoli cells from 8-day-old rats but hardly detectable in cells from 14 and 28 days of age. FSH stimulated c-myc mRNA expression in the primary Sertoli cells derived from only 8- and 14-day-old rats but had almost no effect in the 28-day-old rats. These results suggest that FSH induces c-myc mRNA levels in the primary Sertoli cells from prepubertal and early pubertal rats, and then transient expression of c-myc may be responsible for some roles in the regulation of FSH-dependent genes in Sertoli cells.
authors.affiliation: Department of Biochemistry, School of Medicine, Chungnam National University, Daejeon, Korea.
authors.forename: K
authors.initials: K
authors.lastname: Lim
authors.forename: B D
authors.initials: BD
authors.lastname: Hwang
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D016259:Genes, myc; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells / Q000378:metabolism*; D012741:Sexual Maturation
chemical_list: D012333:RNA, Messenger; D003609:Dactinomycin; D005576:Colforsin; D003994:Bucladesine; D005640:Follicle Stimulating Hormone; D003513:Cycloheximide