title: Genomic reorganization and disrupted chromosomal synteny in the siamang (Hylobates syndactylus) revealed by fluorescence in situ hybridization.
abstract: We employed in situ hybridization ("chromosome painting") of chromosome-specific DNA libraries of all human chromosomes to establish homologies between the human and siamang karyotypes (Hylobates syndactylus, 2n = 50). Numerous intra- and interchromosomal rearrangements have led to a massive reorganization of the siamang karyotype. There have been a minimum of 33 translocations. The 24 siamang autosomes are composed of 60 recognizable segments that show DNA homology to regions of the 22 human autosomes. Only two autosomes have not been involved in translocations. The siamang presents a case, in a primate closely related to humans, in which chromosome morphology and synteny are highly disturbed in a manner similar to that encountered among rodents.
authors.affiliation: Institute of Anthropology and Human Genetics, Munich, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Koehler
authors.forename: N
authors.initials: N
authors.lastname: Arnold
authors.forename: J
authors.initials: J
authors.lastname: Wienberg
authors.forename: S
authors.initials: S
authors.lastname: Tofanelli
authors.forename: R
authors.initials: R
authors.lastname: Stanyon
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002875:Chromosomes / Q000648:ultrastructure*; D004247:DNA / Q000235:genetics; D005260:Female; D015723:Gene Library; D016678:Genome; D006801:Humans; D006922:Hylobates / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008297:Male; D011110:Polymorphism, Genetic; D012689:Sequence Homology, Nucleic Acid; D014178:Translocation, Genetic
chemical_list: D004247:DNA