title: Stem cell defects in parthenogenetic peri-implantation embryos.
abstract: Mouse embryos containing only maternal chromosomes (parthenotes) develop abnormally in vivo, usually failing at the peri-implantation stage. We have analyzed the development of parthenote embryos by using an inner cell mass (ICM) outgrowth assay that mimics peri-implantation development. ICMs from normal embryos maintained undifferentiated stem cells positive for stage-specific embryonic antigen-1 and Rex-1 while differentiating into a variety of cell types, including visceral endoderm-like cells and parietal endoderm cells. In contrast, ICMs from parthenotes failed to maintain undifferentiated stem cells and differentiated almost exclusively into parietal endoderm. This suggests that parthenote ICMs have a defect that leads to differentiation, rather than maintenance, of the stem cells, and a defect that leads to a parietal endoderm fate for the stem cells. To test the hypothesis that the ICM population is not maintained owing to a lack of proliferation of the stem cells, we investigated whether mitogenic agents were able to maintain the ICM population in parthenotes. When parthenote blastocysts were supplied with the insulin-like growth factor-1 receptor (Igf-1r) and insulin-like growth factor-2 (Igf-2), two genes not detectable in parthenote blastocysts by in situ hybridization, the ICM population was maintained. Similarly, culture of parthenote blastocysts in medium conditioned by embryonic fibroblasts and supplemented with the maternal factor leukemia inhibitory factor maintained the ICM population. However, once this growth factor-rich medium was removed, the parthenote ICM cells still differentiated predominantly into parietal endoderm.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Laboratory of Radiobiology and Environmental Health, University of California, San Francisco 94143-0750, USA.
authors.forename: E D
authors.initials: ED
authors.lastname: Newman-Smith
authors.forename: Z
authors.initials: Z
authors.lastname: Werb
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001755:Blastocyst / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D004707:Endoderm / Q000166:cytology; D015870:Gene Expression; D006133:Growth Substances / Q000235:genetics; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010312:Parthenogenesis; D013234:Stem Cells / Q000166:cytology*
chemical_list: D006133:Growth Substances