Heat shock protein expression in oral epithelial dysplasia and squamous cell carcinoma.
Heat shock protein (HSP) expression is upregulated in tumour cells and, therefore, HSP expression is a likely marker of the malignant potential of oral epithelial lesions. Furthermore, the 70-kDa HSP (HSP 70) is implicated in the degree of tumour differentiation, the rate of tumour proliferation and the magnitude of the anti-tumour immune response. Accordingly, the distribution and intensity of HSP 70 expression was assessed in the epithelial compartment of oral squamous cell carcinoma (SCC, n = 29), dysplastic oral epithelium (n = 18) and benign oral mucosal lesions (n = 22) using avidin-biotin complex immunohistochemistry and microdensitometry under standardised conditions. Staining intensity was recorded in kilo-ohms (k omega). Normal oral mucosa (n = 15) was used for comparison, and results were analysed using Kruskall-Wallis and Fisher's exact tests. The distribution of HSP 70 expression in well differentiated SCC was significantly different from that in poorly differentiated SCC (P < 0.05), the latter demonstrating a more focal staining pattern. Median staining intensity in SCC (6.22 k omega), epithelial dysplasia (9.61 k omega) and the benign oral mucosal lesions (8.28 k omega) was significantly greater than that in normal oral mucosa (5.64 k omega; P < 0.05). Staining intensity in poorly differentiated SCC (7.66 k omega) was greater than that in moderately differentiated SCC (4.77 k omega), although this result just failed to reach statistical significance (P = 0.06). These results suggest that, as employed currently, HSP 70 expression is not a definitive marker of oral malignancy or malignant potential.(ABSTRACT TRUNCATED AT 250 WORDS)
Sugerman
P B
PB
Department of Dentistry, University of Queensland, Brisbane, Australia.
Savage
N W
NW
Xu
L J
LJ
Walsh
L J
LJ
Seymour
G J
GJ
D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000737:chemistry*; D004848:Epithelium / Q000737:chemistry; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D009062:Mouth Neoplasms / Q000737:chemistry*; D011230:Precancerous Conditions / Q000737:chemistry*
D014408:Biomarkers, Tumor; D018840:HSP70 Heat-Shock Proteins