Phenotypic properties and cytokine production of skin-infiltrating cells obtained from guinea pig delayed-type hypersensitivity reaction sites.
In this study, skin-infiltrating cells were prepared from guinea pig delayed-type hypersensitivity reaction sites, and their phenotypic properties and cytokine production were examined. Our cell preparations contained around 45% nonspecific esterase-positive cells and a few skin-derived cells. Skin-infiltrating cells produced TNF activity and macrophage chemotactic activity (MCA), whereas resident skin cells produced much lower activity. The highest TNF activity was produced by skin-infiltrating cells at 2 hr after antigen injection. In contrast, a larger amount of MCA was produced by skin-infiltrating cells at 24 and 48 hr than at 2 hr after antigen injection. These kinetics were in good agreement with our previous results regarding cytokine activity in skin extracts. Gel filtration analysis and neutralization assay with a monoclonal antibody against human macrophage chemotactic factor with a molecular weight of 1 kDa (MCF-1) indicated that a guinea pig homologue of human MCF-1 was partially responsible for the MCA.
Higashi
N
N
Kao Institute of Fundamental Research, Tochigi, Japan.
Yoshizuka
N
N
Kobayashi
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000921:Antibody-Producing Cells / Q000276:immunology; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000096:biosynthesis / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008264:Macrophages; D012867:Skin / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000276:immunology
D000911:Antibodies, Monoclonal; D002630:Chemotactic Factors; D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha