title: Lack of serotype-specific antibody response to lipopolysaccharide antigens of Moraxella catarrhalis during lower respiratory tract infection.
abstract: An enzyme immunoassay (EIA) was used to determine the antibody response to different serotypes of lipopolysaccharide (LPS) antigens of Moraxella catarrhalis in adult patients with lower respiratory tract infections (LRTI). Moraxella catarrhalis was isolated from sputum or nasopharyngeal samples from 20 patients with LRTI. Sixteen of the isolates were serotype A, four were type B and none were type C. The antibody response to the different LPS serotypes was determined in paired sera from patients suffering from LRTI. In addition to the 20 patients with Moraxella catarrhalis isolated (Group 1), a group of seven patients with LRTI of unknown etiology (Group 2) and a group of ten patients with LRTI of known other bacterial etiology (Group 3) were selected for this study. An increase in antibody levels of > 1.5-fold (convalescent-/acute-phase serum) was recorded in approximately half of the patients, not only in the first group (Moraxella catarrhalis isolated) but also in the other two groups. However, in the first and second groups there was a correlation between an increase in antibody levels in the LPS EIA and in an EIA using whole bacterial cells as antigen. In the group of patients in whom Moraxella catarrhalis was isolated, the antibody response to LPS antigens was not serotype specific. The antibody response to type-A and type-B LPS was more predominant than the response to type-C LPS in most patients.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Microbiology and Tumorbiology Centre, Karolinska Institute, Stockholm, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Rahman
authors.forename: T
authors.initials: T
authors.lastname: Holme
authors.forename: I
authors.initials: I
authors.lastname: Jönsson
authors.forename: A
authors.initials: A
authors.lastname: Krook
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial / Q000032:analysis* / Q000096:biosynthesis; D000942:Antigens, Bacterial / Q000032:analysis* / Q000096:biosynthesis; D002240:Carbohydrate Sequence; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000032:analysis* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D001936:Moraxella catarrhalis / Q000276:immunology* / Q000302:isolation & purification; D016870:Neisseriaceae Infections / Q000276:immunology*; D011446:Prospective Studies; D012141:Respiratory Tract Infections / Q000276:immunology*; D012680:Sensitivity and Specificity; D012703:Serotyping
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides