PCR-mediated recombination and mutagenesis. SOEing together tailor-made genes.
Gene Splicing by Overlap Extension (gene SOEing) is a sequence-independent method for site-directed mutagenesis and/or recombination of DNA molecules. It is based on the idea that a PCR product can be engineered by adding or changing sequences at its ends so that the product can itself be used to prime DNA synthesis in a subsequent overlap-extension reaction to create mutant or recombinant molecules. As the engineered genes are created in vitro without reliance on host organisms or restriction sites, gene SOEing provides a powerful and versatile tool for genetic investigation and engineering.
Horton
R M
RM
Department of Biochemistry, College of Biological Sciences, St. Paul, MN 55108, USA.
D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000235:genetics*; D011995:Recombination, Genetic
D017931:DNA Primers; D011994:Recombinant Proteins