title: Experimental leprosy in monkeys. II. Longitudinal serological observations in sooty mangabey monkeys.
abstract: In this study, 11 SMM were grouped and inoculated with differing doses of SMM-origin Mycobacterium leprae (ML) between 4.5 x 10(8) and 1 x 10(9) by either combined IV/IC routes or by IV or IC route alone. The combined route was the most effective in eliciting progressive, disseminated LL leprosy. In all, 6 of 7 SMM inoculated by the combined routes developed leprosy requiring treatment at some point. Only 1 of 4 inoculated by a single route developed persisting leprosy requiring chemotherapy. Either no disease or spontaneous regression of initial disease occurred in the other 3 animals inoculated by a single route. Doses in excess of 1 x 10(9) ML were more effective than lesser doses. An association was observed between the development of IgG anti-PGL-I ELISA OD values and resistance to leprosy and between IgM anti-PGL-I and leprosy progression or susceptibility. Serum PGL-I antigen levels, determined by dot ELISA, paralleled disease severity longitudinally. High positive OD values of anti-LAM IgG prior to ML inoculation were observed in the majority of leprosy-susceptible SMM in contrast to negative levels in more resistant animals. Anti-LAM IgG OD values exceeded the positive cut-off point after inoculation in 5 of 11 SMM; 3 of these 5 had concurrent detectable serum levels of PGL-I antigen.
authors.affiliation: Department of Microbiology, Tulane Regional Primate Research Center, Covington, LA, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Gormus
authors.forename: K
authors.initials: K
authors.lastname: Xu
authors.forename: S N
authors.initials: SN
authors.lastname: Cho
authors.forename: G B
authors.initials: GB
authors.lastname: Baskin
authors.forename: R P
authors.initials: RP
authors.lastname: Bohm
authors.forename: L N
authors.initials: LN
authors.lastname: Martin
authors.forename: J L
authors.initials: JL
authors.lastname: Blanchard
authors.forename: P A
authors.initials: PA
authors.lastname: Mack
authors.forename: M S
authors.initials: MS
authors.lastname: Ratterree
authors.forename: W M
authors.initials: WM
authors.lastname: Meyers
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D016665:Cercocebus atys; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007918:Leprosy / Q000276:immunology*; D008070:Lipopolysaccharides / Q000032:analysis*; D008137:Longitudinal Studies; D009166:Mycobacterium leprae / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D006017:Glycolipids; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; C050016:lipoarabinomannan; C045169:phenolic glycolipid I, Mycobacterium leprae