Activation of HIV expression by CD30 triggering in CD4+ T cells from HIV-infected individuals.
CD30 is a member of the tumor necrosis factor receptor superfamily, preferentially expressed by T cells producing type 2 helper (Th2) cytokines, whose ligand (CD30L) has been identified on B cells, activated macrophages, and a subset of activated T cells. We show here that cross-linking CD30 with an agonistic CD30-specific monoclonal antibody, as well as with CD30L+ CD8+ T cell clones or CD30L+ B cells, enhanced HIV replication in CD4+ T cells from HIV-infected individuals, and such a potentiating effect was inhibited by anti-CD30L antibody. The anti-CD30L antibody also exerted a suppressive effect on spontaneous HIV replication occurring in lymph node cells from an HIV-sero-positive patient, showing CD30L expression by both B and CD8+ T lymphocytes. Thus, CD30 triggering by CD30L-expressing cells may plan an important role in the activation of HIV expression from latently infected CD4+ T cells.
Maggi
E
E
Istituto di Clinica Medica 3, University of Florence, Italy.
Annunziato
F
F
Manetti
R
R
Biagiotti
R
R
Giudizi
M G
MG
Ravina
A
A
Almerigogna
F
F
Boiani
N
N
Alderson
M
M
Romagnani
S
S
D001402:B-Lymphocytes / Q000502:physiology; D017252:CD3 Complex / Q000502:physiology; D053285:CD30 Ligand; D015496:CD4-Positive T-Lymphocytes / Q000382:microbiology*; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology; D002478:Cells, Cultured; D015658:HIV Infections / Q000382:microbiology*; D015497:HIV-1 / Q000254:growth & development*; D006801:Humans; D016130:Immunophenotyping; D066298:In Vitro Techniques; D017730:Ki-1 Antigen / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000502:physiology*; D015398:Signal Transduction; D014779:Virus Replication
D017252:CD3 Complex; D053285:CD30 Ligand; D017730:Ki-1 Antigen; D008562:Membrane Glycoproteins; C505774:TNFSF8 protein, human