title: Molecular characterization of the genes encoding the tungsten-containing aldehyde ferredoxin oxidoreductase from Pyrococcus furiosus and formaldehyde ferredoxin oxidoreductase from Thermococcus litoralis.
abstract: The hyperthermophilic archaea Pyrococcus furiosus and Thermococcus litoralis contain the tungstoenzymes aldehyde ferredoxin oxidoreductase, a homodimer, and formaldehyde ferredoxin oxidoreductase, a homotetramer. herein we report the cloning and sequencing of the P. furiosus gene aor (605 residues; M(r), 66,630) and the T. litoralis gene for (621 residues; M(r), 68,941).
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Georgia, Athens 30602, USA.
authors.forename: A
authors.initials: A
authors.lastname: Kletzin
authors.forename: S
authors.initials: S
authors.lastname: Mukund
authors.forename: T L
authors.initials: TL
authors.lastname: Kelley-Crouse
authors.forename: M K
authors.initials: MK
authors.lastname: Chan
authors.forename: D C
authors.initials: DC
authors.lastname: Rees
authors.forename: M W
authors.initials: MW
authors.lastname: Adams
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000145:classification / Q000235:genetics*; D000595:Amino Acid Sequence; D001105:Archaea / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D008667:Metalloproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014414:Tungsten
chemical_list: D008667:Metalloproteins; D000445:Aldehyde Oxidoreductases; C080010:aldehyde ferredoxin oxidoreductase; C080011:formaldehyde ferredoxin oxidoreductase; D014414:Tungsten