title: Novel mutation in the UL97 gene of a clinical cytomegalovirus strain conferring resistance to ganciclovir.
abstract: Characterization of a ganciclovir-resistant cytomegalovirus strain from a patient with AIDS showed a histidine-to-glutamine change at residue 520 of UL97 (Q520 mutation). In anabolism studies, Q520 was associated with impaired phosphorylation of ganciclovir. Transfer of Q520 to a recombinant virus resulted in a ganciclovir-resistant phenotype.
authors.affiliation: Department of Laboratory Medicine, University of Minnesota Medical School, Minneapolis 55455-0392, USA.
authors.forename: M N
authors.initials: MN
authors.lastname: Hanson
authors.forename: L C
authors.initials: LC
authors.lastname: Preheim
authors.forename: S
authors.initials: S
authors.lastname: Chou
authors.forename: C L
authors.initials: CL
authors.lastname: Talarico
authors.forename: K K
authors.initials: KK
authors.lastname: Biron
authors.forename: A
authors.initials: A
authors.lastname: Erice
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology; D001483:Base Sequence; D003587:Cytomegalovirus / Q000187:drug effects* / Q000235:genetics*; D004352:Drug Resistance, Microbial; D015774:Ganciclovir / Q000494:pharmacology*; D005973:Glutamine / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D016133:Polymerase Chain Reaction
chemical_list: D005973:Glutamine; D006639:Histidine; D015774:Ganciclovir