title: A distal Sp1-element is necessary for maximal activity of the human gastrin gene promoter.
abstract: Studies of transgenic mice have shown that transcriptional control of the gastrin gene exhibits significant species differences. Transfection of the human gastrin promoter in murine cells have depicted proximal Sp1, E-box and CACC elements as the major determinants of transcription. We have examined cis-regulatory elements of the human promoter on a human gastrin expressing cell line and find that a distal -135 to -142 Sp1 element is necessary for maximal activity. Alignment of the mouse and human promoters shows that the proximal human Sp1 and CACC elements are not conserved, whereas the E-box element is retained. The distal Sp1 element is present in mouse but exhibits a C to T transition in the core that is likely to reduce binding affinity of Sp1. We conclude that gastrin gene transcription is regulated by distinct elements in man and rodents.
authors.affiliation: Department of Clinical Biochemistry, Rigshospitalet, Copenhagen, Denmark.
authors.forename: J R
authors.initials: JR
authors.lastname: Bundgaard
authors.forename: T O
authors.initials: TO
authors.lastname: Hansen
authors.forename: L
authors.initials: L
authors.lastname: Friis-Hansen
authors.forename: I J
authors.initials: IJ
authors.lastname: Rourke
authors.forename: W W
authors.initials: WW
authors.lastname: van Solinge
authors.forename: F C
authors.initials: FC
authors.lastname: Nielsen
authors.forename: J F
authors.initials: JF
authors.lastname: Rehfeld
mesh_terms: D000230:Adenocarcinoma; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism; D005755:Gastrins / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D013274:Stomach Neoplasms; D014158:Transcription, Genetic / Q000235:genetics*; D014407:Tumor Cells, Cultured
chemical_list: D005755:Gastrins; D016329:Sp1 Transcription Factor; D004247:DNA