title: Cloning and characterization of a cDNA encoding a cellobiose dehydrogenase from the white rot fungus Phanerochaete chrysosporium.
abstract: The cDNA of cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium has been cloned and sequenced. The 5' end was obtained by PCR amplification. The cDNA contains 2310 translated bases excluding the poly(A) tail. The deduced mature protein contains 770 amino acid residues and is preceded by a 18 residue long signal peptide. The regions of the amino acid sequence corresponding to the heme and FAD domains of CDH were identified as well as the nucleotide-binding motif, the disulfide pairing and a methionine residue chelating the heme iron. No homologous sequences were found for the heme domain, however, the FAD domain appears to be distantly related to the GMC oxidoreductase family.
authors.affiliation: Centro de Ingeniéria Genética y Biotecnologia, Havana, Cuba.
authors.forename: M
authors.initials: M
authors.lastname: Raices
authors.forename: E
authors.initials: E
authors.lastname: Paifer
authors.forename: J
authors.initials: J
authors.lastname: Cremata
authors.forename: R
authors.initials: R
authors.lastname: Montesino
authors.forename: J
authors.initials: J
authors.lastname: Ståhlberg
authors.forename: C
authors.initials: C
authors.lastname: Divne
authors.forename: I J
authors.initials: IJ
authors.lastname: Szabó
authors.forename: G
authors.initials: G
authors.lastname: Henriksson
authors.forename: G
authors.initials: G
authors.lastname: Johansson
authors.forename: G
authors.initials: G
authors.lastname: Pettersson
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001483:Base Sequence; D001487:Basidiomycota / Q000201:enzymology / Q000235:genetics*; D002237:Carbohydrate Dehydrogenases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D005182:Flavin-Adenine Dinucleotide; D005800:Genes, Fungal / Q000235:genetics*; D006418:Heme; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D000596:Amino Acids; D018076:DNA, Complementary; D004271:DNA, Fungal; D005182:Flavin-Adenine Dinucleotide; D006418:Heme; D002237:Carbohydrate Dehydrogenases; C019859:cellobiose-quinone oxidoreductase