Inactivation of rsmA leads to overproduction of extracellular pectinases, cellulases, and proteases in Erwinia carotovora subsp. carotovora in the absence of the starvation/cell density-sensing signal, N-(3-oxohexanoyl)-L-homoserine lactone.
The soft-rotting bacterium, Erwinia carotovora subsp. carotovora 71, produces extracellular enzymes such as pectate lyase isozymes (Pels), cellulase (Cel), polygalacturonase (Peh), and protease (Prt). While the extracellular levels of these enzymes are extremely low when the bacterium is grown in salts-yeast extract-glycerol (SYG) medium, the enzymatic activities are highly induced in SYG medium supplemented with celery extract. By transposon (mini-Tn5) mutagenesis, we isolated a RsmA- mutant, AC5070, which overproduces extracellular enzymes; the basal levels of Pel, Peh, and Cel in AC5070 are higher than the induced levels in the RsmA+ parent, AC5047. While Peh production is mostly constitutive in AC5070, Pel, Cel, and Prt production is still inducible with celery extract. The high basal levels of pel-1, pel-3, and peh-1 mRNAs in AC5070 demonstrate that overproduction of the pectolytic enzymes is due to the stimulation of transcription. Using chromosomal DNA flanking mini-Tn5 as a probe, we cloned the wild-type rsmA+ allele, which suppresses Pel, Peh, Cel, and Prt production in both RsmA+ and RsmA- strains. The RsmA- mutant, like its parent, produces N-(3-oxohexanoyl)-L-homoserine lactone (HSL), a starvation/cell density-sensing signal required for extracellular enzyme production. To examine the role of HSL, we constructed HSL-deficient strains by replacing hslI, a locus required for HSL production, with hslI::Tn3HoHo1-Spc. While the basal levels of Pel, Peh, Cel, and Prt are comparable in the RsmA- mutant and its HSL- derivative, these enzymes are barely detectable in the Hsl- derivative of the RsmA+ parent strain.(ABSTRACT TRUNCATED AT 250 WORDS)
Chatterjee
A
A
Department of Plant Pathology, University of Missouri, Columbia 65211, USA.
Cui
Y
Y
Liu
Y
Y
Dumenyo
C K
CK
Chatterjee
A K
AK
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000235:genetics / Q000502:physiology; D000595:Amino Acid Sequence; D002480:Cellulase / Q000096:biosynthesis* / Q000235:genetics; D003470:Culture Media / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016973:Pectobacterium carotovorum / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D010936:Plant Extracts / Q000494:pharmacology; D011096:Polygalacturonase / Q000096:biosynthesis* / Q000235:genetics; D011133:Polysaccharide-Lyases / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014774:Virulence
D003470:Culture Media; D007527:Isoenzymes; D010936:Plant Extracts; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; C030654:N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone; D011096:Polygalacturonase; D002480:Cellulase; D011133:Polysaccharide-Lyases; C023911:pectate lyase; D015107:4-Butyrolactone