Heterogeneous decrease of bone mineral density in the vertebral column of ovariectomized rats.
The long-term effect of ovariectomy on the loss of bone mineral density (BMD) was evaluated in rats with and without estrogen treatment; BMD was studied in the lumbar and caudal vertebrae, measured by DXA, to find how the losses of BMD occur in the axial skeleton. Seventy female Wistar rats of 3 months of age were divided into four groups as follows: group 1: control animals; group 2: ovariectomized animals; group 3: ovariectomized animals undergoing treatment with estrogen (0.25 mg/kg per week of 17-beta estradiol); group 4: ovariectomized rats undergoing estrogen treatment only during the last 3 months of the experimental period. No significant differences were found among the groups in regard to the BMD values of the caudal vertebrae at either 3 or 6 months. Likewise, in the lumbar vertebrae there were no significant differences among the groups after 3 months. However, at 6 months, a decrease in the BMDs of the ovariectomized animals with respect to the remaining groups was found: 226 +/- 11 mg/cm2 in the ovariectomized group; 262 +/- 14 mg/cm2 in the controls; 255 +/- 4 mg/cm2 in the rats receiving estrogen treatment for 6 months; and 259 +/- 5 mg/cm2 in the animals receiving estrogen for 3 months. The study also reveals the absence of differences in the bone mineral density between the ovariectomized and control rats when the former received estrogen treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
García-Moreno
C
C
Departamento de Fisiología y Farmacología, Universidad de Salamanca, Spain.
Calvo
O M
OM
Herrero
S
S
Martín
E
E
Suquía
B
B
San Román
J I
JI
Martín
M
M
García-Talavera
J R
JR
Calvo
J J
JJ
del Pino
J
J
D015502:Absorptiometry, Photon; D000469:Alkaline Phosphatase / Q000097:blood; D000704:Analysis of Variance; D000818:Animals; D001835:Body Weight / Q000502:physiology; D015519:Bone Density / Q000187:drug effects / Q000502:physiology*; D001846:Bone Development / Q000187:drug effects / Q000502:physiology; D001862:Bone Resorption / Q000503:physiopathology; D002118:Calcium / Q000652:urine; D003404:Creatinine / Q000652:urine; D004195:Disease Models, Animal; D004958:Estradiol / Q000008:administration & dosage / Q000627:therapeutic use; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D006801:Humans; D006909:Hydroxyproline / Q000652:urine; D008159:Lumbar Vertebrae / Q000187:drug effects / Q000502:physiology*; D015675:Osteocalcin / Q000097:blood; D015663:Osteoporosis, Postmenopausal / Q000503:physiopathology; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar; D013131:Spine / Q000187:drug effects / Q000502:physiology*; D013623:Tail
D004967:Estrogens; D015675:Osteocalcin; D004958:Estradiol; D003404:Creatinine; D000469:Alkaline Phosphatase; D006909:Hydroxyproline; D002118:Calcium