Butyrate-inducible and tumor-restricted gene expression by adenovirus vectors.
Efficient introduction of biologically active genes into tumor cells in vivo would facilitate cancer gene therapy. In this study, recombinant adenoviruses mediated high-level expression of reporter genes in both human and murine tumor cell lines and freshly resected human solid tumors in vitro. When adenovirus vectors were inoculated intratumorally in a living animal, reporter activity was detected only in the tumor nodule and not in other sites, in contrast to intracardiac injections in which expression was found in several organs. Adenovirus-mediated gene expression was further enhanced by butyrate treatment.
Tang
D C
DC
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235-8593, USA.
Johnston
S A
SA
Carbone
D P
DP
D000260:Adenoviruses, Human / Q000235:genetics*; D000818:Animals; D002087:Butyrates / Q000494:pharmacology*; D020148:Butyric Acid; D002277:Carcinoma / Q000473:pathology / Q000628:therapy*; D003587:Cytomegalovirus / Q000235:genetics; D003673:Defective Viruses / Q000235:genetics*; D004742:Enhancer Elements, Genetic; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D017930:Genes, Reporter; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics*; D006801:Humans; D015552:Injections, Intralesional; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008175:Lung Neoplasms / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008845:Microinjections / Q000295:instrumentation; D009368:Neoplasm Transplantation; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis*; D014162:Transfection; D014407:Tumor Cells, Cultured; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D002087:Butyrates; D011993:Recombinant Fusion Proteins; D020148:Butyric Acid; D008156:Luciferases; D001616:beta-Galactosidase