title: Purification and cloning of DNA fragments fractionated on agarose gels.
abstract: Purification of DNA fragments from acrylamide or agarose gels is a commonly used technique in the molecular biology laboratory. This article describes a rapid, efficient, and inexpensive method of purifying DNA fractions from an agarose gel. The purified DNA is suitable for use in a wide range of applications including ligation using DNA ligase. The procedure uses standard high-melting-temperature agarose and normal TBE electrophoresis buffer. In addition, the protocol does not involve the use of highly toxic organic solvents such as phenol.
authors.affiliation: Genetics and Microbiology Department, Institute of Food Research, Colney, Norwich, UK.
authors.forename: H G
authors.initials: HG
authors.lastname: Griffin
authors.forename: M J
authors.initials: MJ
authors.lastname: Gasson
mesh_terms: D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D013294:Lactococcus lactis
chemical_list: D004269:DNA, Bacterial; D004247:DNA