Effect of dietary antioxidants on the susceptibility to hepatic microsomal lipid peroxidation in the rat.
The inhibitory effect of probucol on ferrous-iron-induced microsomal lipid peroxidation was compared to that of alpha-tocopherol and butylated hydroxytoluene (BHT). Male Wistar rats were fed with diets containing 1% probucol, 0.2% BHT or 0.1% alpha-tocopherol for 30 days. There were no effects of dietary antioxidants on growth parameters, although liver weight was significantly higher in the BHT-fed rats. Probucol reduced serum levels of total and free cholesterol, while BHT feeding increased the concentrations of serum thiobarbituric-acid-reactive substances, HDL cholesterol and hepatic phospholipid. alpha-Tocopherol had no effect on these parameters. Incorporation of both, probucol and alpha-tocopherol, decreased the susceptibility of microsomes to lipid peroxidation in vitro, while BHT incorporation increased hepatic microsomal lipid peroxidation. These results suggest the possible usefulness of probucol for treatment of both hypercholesterolemia and elevated hepatic microsomal lipid peroxidation, while alpha-tocopherol decreases only an elevated lipid peroxidation. BHT works as a prooxidant.
Yamamoto
K
K
Department of Internal Medicine, Saga Medical University School of Medicine, Japan.
Fukuda
N
N
Shiroi
S
S
Shiotsuki
Y
Y
Nagata
Y
Y
Tani
T
T
Sakai
T
T
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002784:Cholesterol / Q000097:blood; D004032:Diet; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism; D005527:Food, Fortified; D006937:Hypercholesterolemia / Q000097:blood / Q000188:drug therapy / Q000378:metabolism; D050356:Lipid Metabolism; D015227:Lipid Peroxidation / Q000187:drug effects* / Q000502:physiology; D008099:Liver / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism; D011341:Probucol / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014810:Vitamin E / Q000008:administration & dosage / Q000494:pharmacology
D000975:Antioxidants; D005227:Fatty Acids; D010743:Phospholipids; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D002084:Butylated Hydroxytoluene; D002784:Cholesterol; D011341:Probucol