title: Expression of protein kinase C delta gene in germ cells.
abstract: OBJECTIVE
  To investigate the biological functions of protein kinase C delta (PKC delta) in spermatogenesis.
  
  
  METHODS
  We examined PKC delta transcript in mouse testis by means of in situ hybridization and Northern blotting.
  
  
  RESULTS
  In testes of normal mice, signals of PKC delta gene expression were detected specifically at the spermatid stage. The PKC delta gene was weakly expressed in 8-week-old mice and highly expressed by 12 weeks. However, the expression was not detected in testes of germ cell-deficient W/Wv mice even at 12 weeks.
  
  
  CONCLUSIONS
  Protein kinase C delta gene expression may be controlled by specific developmental processes and PKC delta may play a role in spermatogenesis.
authors.affiliation: Department of Microbiology and Immunology, Wonkwang University School of Medicine, Iksan, Korea.
authors.forename: J Y
authors.initials: JY
authors.lastname: Um
authors.forename: B M
authors.initials: BM
authors.lastname: Choi
authors.forename: J S
authors.initials: JS
authors.lastname: Kim
authors.forename: J S
authors.initials: JS
authors.lastname: Rim
authors.forename: H M
authors.initials: HM
authors.lastname: Kim
authors.forename: H T
authors.initials: HT
authors.lastname: Chung
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D015971:Gene Expression Regulation, Enzymologic; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000235:genetics* / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011493:Protein Kinase C / Q000235:genetics* / Q000502:physiology; D051745:Protein Kinase C-delta; D012333:RNA, Messenger / Q000032:analysis; D013091:Spermatogenesis / Q000502:physiology; D013094:Spermatozoa / Q000737:chemistry*; D013737:Testis / Q000166:cytology; D014158:Transcription, Genetic
chemical_list: D007527:Isoenzymes; D012333:RNA, Messenger; C497259:Prkcd protein, mouse; D011493:Protein Kinase C; D051745:Protein Kinase C-delta