Plasma level determination of 1,4-butanedisulphonate by ion chromatography and conductimetric detection.
A rapid, specific and reproducible liquid chromatographic method was developed for the determination of 1,4-butanedisulphonate in plasma. The method involves protein precipitation with perchloric acid, precipitation of perchlorate ions by addition of potassium carbonate followed by ion chromatography on an ion-exchange column connected with a conductimetric detector. Calibration graphs were linear over the concentration range 2.5-25 micrograms/ml; the intra-assay precision was < or equal to 3.6% and the inter-assay precision was < or equal to 5.8%. The analyte was stable in plasma and in perchloric acid at 37 degrees C for 24 h. The assay procedure was applied to monitoring plasma levels in animals receiving chronic intravenous and oral administration of the analyte.
Moro
E
E
Department of Pharmacokinetics, Research and Development, BioResearch SpA, Liscate, Italy.
De Angelis
M
M
Fugazza
B
B
D000476:Alkanesulfonates / Q000097:blood*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D007477:Ions; D051381:Rats; D015203:Reproducibility of Results
D000476:Alkanesulfonates; D007477:Ions; C092739:1,4-butanedisulfonate