Interruption of the ferredoxin (flavodoxin) NADP+ oxidoreductase gene of Escherichia coli does not affect anaerobic growth but increases sensitivity to paraquat.
Ferredoxin (flavodoxin) NADP+ oxidoreductase participates in methionine biosynthesis and in the function of two anaerobic enzymes, pyruvate formate-lyase and ribonucleotide reductase. We prepared insertion mutants of Escherichia coli lacking a functional enzyme. They do not require methionine and they grow well anaerobically, but they show increased sensitivity to paraquat.
Bianchi
V
V
Department of Genetics, Stockholm University, Sweden.
Haggård-Ljungquist
E
E
Pontis
E
E
Reichard
P
P
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D000693:Anaerobiosis; D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D005287:Ferredoxin-NADP Reductase / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010269:Paraquat / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics; D012264:Ribonucleotide Reductases / Q000235:genetics / Q000378:metabolism
D004251:DNA Transposable Elements; D012264:Ribonucleotide Reductases; D005287:Ferredoxin-NADP Reductase; D000123:Acetyltransferases; C022560:formate C-acetyltransferase; D010269:Paraquat