A rapid test for measuring the infectivity of Yellow Fever vaccine.
An in-house reference preparation of 17D-204 Yellow Fever vaccine was tested 60 times using LD50 mouse assay and CCID50 method. The mean virus titre, standard deviation and coefficient of variation obtained were comparable. Student's t-test indicated that there was a statistically insignificant difference between the two methods (p > 0.05) used for potency test with a correlation coefficient of 0.666 (p < 0.05). CCID50 assay for measuring the infectivity of Yellow Fever vaccine is rapid, sensitive and reproducible.
Sood
D K
DK
Central Drugs Laboratory, Central Research Institute, Kasauli- India.
Aggarwal
R K
RK
Kumar
S
S
Sokhey
J
J
D000818:Animals; D002522:Chlorocebus aethiops; D005069:Evaluation Studies as Topic; D005260:Female; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014709:Vero Cells; D010948:Viral Plaque Assay; D014765:Viral Vaccines / Q000494:pharmacology* / Q000633:toxicity; D015005:Yellow fever virus / Q000276:immunology*
D014765:Viral Vaccines