Purification and characterization of xenorhabdicin, a phage tail-like bacteriocin, from the lysogenic strain F1 of Xenorhabdus nematophilus.
Xenorhabdicin, the phage tail-like bacteriocins of Xenorhabdus nematophilus, and phage head particles, elements produced together after mitomycin induction in X. nematophilus lysogenic strain F1 cultures, were separated by DEAE chromatography, examined by transmission electron microscopy, and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Electrophoresis of xenorhabdicin showed two major subunits of 43 and 20 kDa corresponding to the sheath and the inner core, respectively. At least five other minor subunits of 67, 54, 35, 28, and 16 kDa were also characterized. Electrophoresis of the phage head capsids showed a major 40-kDa subunit and two minor 50- and 34-kDa subunits. Bactericidal activity recorded against closely related bacterial species and spontaneously produced by X. nematophilus resides in the xenorhabdicin particles and is another antimicrobial barrier to save the symbiotic association.
Thaler
J O
JO
Laboratoire de Pathologie Comparée, Université de Montpelier II, France.
Baghdiguian
S
S
Boemare
N
N
D000818:Animals; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001435:Bacteriophages / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure; D002213:Capsid / Q000737:chemistry / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000254:growth & development / Q000378:metabolism* / Q000821:virology; D007313:Insecta / Q000469:parasitology; D008242:Lysogeny; D016685:Mitomycin / Q000494:pharmacology; D008970:Molecular Weight; D009348:Nematoda / Q000382:microbiology; D013559:Symbiosis
D001430:Bacteriocins; D016685:Mitomycin