The endothelium-derived relaxing factor-mediated acetylcholine response of the arterial perfusion pressure after cold storage of the isolated rabbit kidney.
The vasodilatation induced by acetylcholine (ACh) in a rabbit isolated perfused kidney was abolished when the tissue was exposed to cold ischemia for 72 h in Euro-Collins (EC) solution. This vasodilatation is due to the release of endothelium-derived relaxing factor (EDRF) from renal vasculature as evidenced by the attenuation following methylene blue pretreatment. When kidneys were preserved in EC solution containing UK 38485, a thromboxane synthase inhibitor, or nicardipine, a calcium channel blocker, ACh-induced vasodilatation persisted after 72 h of cold ischemia. These results were taken as evidence of tissue protective activity of UK 38485 and nicardipine and have promising implications for cadaveric kidney transplantation.
Kuzu
A
A
Department of Surgery, University of Ankara, Turkey.
Alaçayir
I
I
Köksoy
C
C
Yazar
O
O
Kuterdem
E
E
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001158:Arteries / Q000503:physiopathology; D003080:Cold Temperature; D007668:Kidney / Q000098:blood supply / Q000503:physiopathology*; D016030:Kidney Transplantation; D009569:Nitric Oxide / Q000378:metabolism* / Q000494:pharmacology; D009926:Organ Preservation; D010477:Perfusion; D011817:Rabbits; D014664:Vasodilation / Q000187:drug effects*
D009569:Nitric Oxide; D000109:Acetylcholine