Use of the polymerase chain reaction for the detection of Chlamydia trachomatis in clinical specimens and its comparison to commercially available tests.
A polymerase chain reaction (PCR) was developed to detect Chlamydia trachomatis in genital tract specimens. Two sets of primers for the PCR were used; one set amplifies a region of the plasmid present in all C trachomatis strains and the other amplifies a conserved region of the genome coding for the major outer membrane protein. The sensitivity of these PCRs were compared with each other, and with the sensitivities of antigen ELISA, Clearview and culture. Southern blotting and probing was used to increase sensitivity of detection.
Martin
J L
JL
Department of Microbiology, Monash University Medical School, Prahran, Victoria, Australia.
Alexander
S Y
SY
Selwood
T S
TS
Cross
G F
GF
D001431:Bacteriological Techniques; D001483:Base Sequence; D015139:Blotting, Southern; D002584:Cervix Uteri / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity