title: [Immunocytochemical analysis of human prolactin receptors using anti-idiotypic antibodies in human breast cancer].
abstract: In this study, we have prepared antiidiotypic antibodies specific of human prolactin receptors (PRL-R) in order to localize these receptors in breast cancer. Antiidiotypic antibodies were prepared using anti-human prolactin (anti-hPRL) sera obtained from New Zealand rabbits. 25 breast cancer were PRL-R positive using radio receptor assay. For immunohistochemistry, the tissues fixed with Nakane and treated by NH4Cl and Saponin to improve staining specificity, were incubated with antiidiotypic antibodies (1/100e). Cytoplasm and membrane of human breast cancer cells were PRL-R positive in 20/25 breast cancer with antiidiotypic antibodies. There was no staining with preimmune serum and immunohistochemical staining elimination by prior absorption with anti-hPRL antibodies.
authors.affiliation: Laboratoire d'Histologie-Embryologie et Biologie de la Reproduction, Faculté de Médecine, Lille.
authors.forename: B
authors.initials: B
authors.lastname: Leroy-Martin
authors.forename: J P
authors.initials: JP
authors.lastname: Peyrat
authors.forename: S
authors.initials: S
authors.lastname: Amrani
authors.forename: M
authors.initials: M
authors.lastname: Lorthioir
authors.forename: J
authors.initials: J
authors.lastname: Leonardelli
mesh_terms: D000888:Antibodies, Anti-Idiotypic; D000918:Antibody Specificity; D001943:Breast Neoplasms / Q000175:diagnosis*; D006801:Humans; D007150:Immunohistochemistry; D011237:Predictive Value of Tests; D011981:Receptors, Prolactin / Q000032:analysis*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D011981:Receptors, Prolactin
vernacular_title: Analyse immunocytochimique des récepteurs prolactiniques (R-PRL) humains à l'aide d'anticorps antiidiotypes dans les cancers du sein humain.