title: OK-432 induces production of neutrophil chemotactic factors in malignant pleural effusion.
abstract: We investigated the changes in cellular components and neutrophil chemotactic factors in pleural fluid from 19 lung cancer patients who received intrapleural injection of OK-432 to treat malignant pleurisy. Not only neutrophil chemotactic activity (NCA) but also neutrophil count and percentage were increased significantly at 6 hours after OK-432 injection. The neutrophil count was significantly correlated with NCA level. The levels of C5a and IL-8 in pleural fluid were increased significantly after OK-432 injection. The increased IL-8 level was associated with a increase of both NCA and neutrophil count. OK-432 treatment also induced a marked increase of IL-1 beta and IL-6 in pleural fluid. Thus, intrapleural injection of OK-432 induced production of neutrophil chemotactic factors (IL-8 and C5a) and cytokines (IL-1 beta and IL-6), which eventually attracted neutrophils into the pleural space. These observations suggest that neutrophil migration mediated by these factors and cytokines may contribute to the sclerosing effects of OK-432 treatment.
authors.affiliation: Second Department of Internal Medicine, Okayama University Medical School.
authors.forename: M
authors.initials: M
authors.lastname: Kataoka
authors.forename: R
authors.initials: R
authors.lastname: Morishita
authors.forename: J
authors.initials: J
authors.lastname: Hiramatsu
authors.forename: H
authors.initials: H
authors.lastname: Ueoka
authors.forename: Y
authors.initials: Y
authors.lastname: Nakata
authors.forename: T
authors.initials: T
authors.lastname: Ohnoshi
authors.forename: I
authors.initials: I
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Harada
mesh_terms: D000328:Adult; D000368:Aged; D002630:Chemotactic Factors / Q000096:biosynthesis*; D015936:Complement C5a / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D007958:Leukocyte Count; D008175:Lung Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D010844:Picibanil / Q000627:therapeutic use*; D016066:Pleural Effusion, Malignant / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D002630:Chemotactic Factors; D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8; D010844:Picibanil; D015936:Complement C5a