Limited heterogeneity in T-cell receptor V beta chain gene expression in arthritic joints of BUB/BnJ (H-2q) mice--a T-cell receptor V beta a strain.
We have earlier shown that T-cells in arthritic joints and LNs of B10.Q mice (H-2q, TCR V beta b) use a restricted number of TCR V beta chain genes (V beta 6, 8, 9). In the present study, we have investigated the TCR V beta chain gene expression in arthritic joints and LN of BUB/BnJ mice (H-2q, TCR V beta a). Mice were immunized with [table: see text] chicken type-II collagen, and arthritic joints and draining LNs were removed at the onset of arthritis and the TCR V beta chain gene expression was studied by PCR. A restricted usage of TCR V beta was observed in both the tissues. A dominant usage of TCR V beta 4, 7, and 15 was found in the LNs while TCR V beta 3 and 10 were predominantly expressed in arthritic joints in the majority of the arthritic mice (5/7). Our results indicate that (a) in H-2q mice with CIA there is a restricted usage of TCR V beta chain genes regardless of the TCR V beta genotype; and (b) in the absence of TCR V beta 8 and 9, TCR V beta 3 and 10 are predominantly used by joint-infiltrating T-cells.
Haqqi
T M
TM
Department of Medicine Case Western Reserve University, Cleveland, Ohio 44106-4946, USA.
Qu
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Banerjee
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D000595:Amino Acid Sequence; D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology* / Q000473:pathology; D001327:Autoimmune Diseases / Q000276:immunology*; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006183:H-2 Antigens / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000473:pathology
D006183:H-2 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta