Sulfated polyanions block Chlamydia trachomatis infection of cervix-derived human epithelia.
Using a cell line derived from the human cervix and a rapid fluorescence cytotoxicity assay, we have shown that Chlamydia trachomatis infection can be blocked by certain sulfated polysaccharides (carrageenan, pentosan polysulfate, fucoidan, and dextran sulfate) and glycosaminoglycans (heparin, heparan sulfate, and dermatan sulfate) but not by other glycosaminoglycans (chondroitin sulfate A or C, keratan sulfate, and hyaluronic acid). The most negatively charged molecules are the most effective at blocking infection. Results of infection at 4 degrees C suggest that sulfated polyanions act by preventing the adherence of chlamydiae to target cells. These and additional blocking studies with enzymes suggest that a heparan sulfate-like glycosaminoglycan on the surface of elementary bodies is involved in the adherence of chlamydiae to target cells, probably through a nonspecific charge interaction or possibly a heparin-binding protein. We previously observed that the same sulfated polysaccharides inhibit transmission of human immunodeficiency virus in vitro and suggested that these compounds could be used in a vaginal formulation to inhibit infection by human immunodeficiency virus. The results of the present study suggest that the same type of formulation may inhibit sexual transmission of chlamydia.
Zaretzky
F R
FR
Population Council, New York, New York 10021, USA.
Pearce-Pratt
R
R
Phillips
D M
DM
D000818:Animals; D002351:Carrageenan / Q000494:pharmacology; D002460:Cell Line; D002584:Cervix Uteri / Q000382:microbiology* / Q000648:ultrastructure; D002692:Chlamydia trachomatis / Q000187:drug effects / Q000502:physiology*; D004848:Epithelium / Q000382:microbiology; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006497:Heparan Sulfate / Q000494:pharmacology; D006801:Humans; D051379:Mice
D002351:Carrageenan; D006493:Heparin; D006497:Heparan Sulfate