Zeolite A inhibits osteoclast-mediated bone resorption in vitro.
The effects of Zeolite A on bone resorbing activity of highly purified avian osteoclasts were analyzed. The present study demonstrates that when 100 micrograms/ml of acid-treated Zeolite A is added to the media the number of pits per osteoclast is reduced 3-fold at 24 h after treatment. Secreted cathepsin B enzyme activity was also reduced 3-fold. A similar reduction in pit number per osteoclast was measured following 48 h of treatment with Zeolite A but there appeared to be less reduction of cathepsin B enzyme activity. The effects on pit number and cathepsin B protein activity were Zeolite dose dependent. The structure of the compound seemed to be responsible for the effects measured since compounds used to represent constituents of Zeolite A (silicon dioxide and aluminum chloride) failed to inhibit bone resorption or reduce the level of secreted cathepsin B enzyme activity. Thus the molecular architecture of Zeolite A or a derivative thereof appears to be important. In conclusion, the data indicate that Zeolite A can inhibit bone resorption. Together with previous data on osteoblasts, this might suggest a potential positive activity of intact Zeolite A or a partial substructure of Zeolite A on bone turnover.
Schütze
N
N
Department of Biochemistry, Mayo Clinic, Rochester, Minnesota 55905, USA.
Oursler
M J
MJ
Nolan
J
J
Riggs
B L
BL
Spelsberg
T C
TC
D000077410:Aluminum Chloride; D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D001862:Bone Resorption / Q000378:metabolism / Q000517:prevention & control*; D001842:Bone and Bones / Q000378:metabolism; D002401:Cathepsin B / Q000378:metabolism; D002645:Chickens; D002712:Chlorides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D066298:In Vitro Techniques; D010010:Osteoclasts / Q000187:drug effects / Q000378:metabolism; D012822:Silicon Dioxide / Q000494:pharmacology; D013997:Time Factors; D017641:Zeolites / Q000494:pharmacology*
D017607:Aluminum Compounds; D002712:Chlorides; D017641:Zeolites; D000077410:Aluminum Chloride; D012822:Silicon Dioxide; D002401:Cathepsin B