Potent antiviral activity of an antisense oligonucleotide complementary to the intron-exon boundary of human cytomegalovirus genes UL36 and UL37.
An antisense phosphorothioate oligonucleotide complementary to the intron-exon boundary of human cytomegalovirus genes UL36 and UL37 (UL36ANTI) reduced the yield of infectious virus by 99% and inhibited human cytomegalovirus DNA replication at a concentration of 0.08 microM. In addition, oligonucleotides with base substitutions which resulted in base pair mismatches showed lesser degrees of activity, indicating a sequence-specific antisense mechanism. UL36ANTI was also shown to inhibit DNA replication of ganciclovir-resistant strains and human cytomegalovirus clinical isolates.
Pari
G S
GS
Hybridon Inc., Worcester, Massachusetts 01605, USA.
Field
A K
AK
Smith
J A
JA
D000998:Antiviral Agents / Q000494:pharmacology*; D001483:Base Sequence; D002470:Cell Survival / Q000187:drug effects; D003587:Cytomegalovirus / Q000187:drug effects* / Q000235:genetics*; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis; D004351:Drug Resistance; D004797:Enzyme-Linked Immunosorbent Assay; D005091:Exons / Q000502:physiology; D005347:Fibroblasts; D015774:Ganciclovir / Q000494:pharmacology; D006801:Humans; D007438:Introns / Q000502:physiology; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D004279:DNA, Viral; D016376:Oligonucleotides, Antisense; D015774:Ganciclovir