Generation of targeted retroviral vectors by using single-chain variable fragment: an approach to in vivo gene delivery.
We report the generation of a retroviral vector that infects human cells specifically through recognition of the low density lipoprotein receptor. The rationale for this targeted infection is to add onto the ecotropic envelope protein of Moloney murine leukemia virus, normally trophic for murine cells, a single-chain variable fragment derived from a monoclonal antibody recognizing the human low density lipoprotein receptor. This chimeric envelope protein was used to construct a packaging cell line producing a retroviral vector capable of high-efficiency transfer of the Escherichia coli beta-galactosidase gene to human cells expressing low density lipoprotein receptor. This approach offers a generalized plan to generate cell and tissue-specific retroviral vectors, an essential step toward in vivo gene therapy strategies.
Somia
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Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, San Diego, CA 92186-5800, USA.
Zoppé
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Verma
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D000818:Animals; D001483:Base Sequence; D002678:Chimera / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D018390:Gene Targeting; D015316:Genetic Therapy; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D008979:Moloney murine leukemia virus / Q000235:genetics*; D011973:Receptors, LDL / Q000235:genetics; D001616:beta-Galactosidase / Q000235:genetics
D017931:DNA Primers; D018076:DNA, Complementary; D011973:Receptors, LDL; D001616:beta-Galactosidase