title: [Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in young patients with acute epididymitis].
abstract: Specimens from 15 young patients presenting with acute epididymitis were tested for the presence of Chlamydia trachomatis by an enzyme immunoassay (EIA), polymerase chain reaction (PCR), and for other bacteria by standard laboratory techniques. C. trachomatis urethral infection was detected in 3 patients by an EIA test of the urethral swabs (20%) and in 13 patients by the PCR (87%). This difference in detection rate was statistically significant (p < 0.005). Thirteen specimens were positive by the PCR, but only three of them were positive by the EIA method. These findings indicate that the PCR assay is a highly sensitive assay for the detection of C. trachomatis in male urine specimens and provides a noninvasive technique for routine screening of chlamydia infection in the patient with acute epididymitis.
authors.affiliation: Department of Urology, Nagoya University School of Medicine.
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Hibi
authors.forename: S
authors.initials: S
authors.lastname: Katsuno
authors.forename: K
authors.initials: K
authors.lastname: Miyake
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D002690:Chlamydia Infections; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D004823:Epididymitis / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity