[Cloning, characterization and expression of mouse ferritin L subunit gene].
We have cloned the functional gene coding for the L ferritin subunit by successive rounds of screening of a mouse genomic library using different oligonucleotides so as to avoid cloning the multiple pseudogenes of this rather complex multigene family. The L gene consists in 4 exons interrupted by 3 introns and spanning 1.8 kb. Quantitative measurements of H and L ferritin mRNA in various mouse tissues using a ribonuclease protection assay reveals important variations in the L/H ratio, the liver displaying the highest amount of L mRNA. Functional analysis of 1 kb of upstream sequence by transient transfections into the hepatoma cell line HepG2 shows that the mouse L gene transcription relies upon a minimal 130 bp promoter region containing 1 TATA box and 2 CCAAT motifs. Elements with an enhancing activity specific of hepatic tissue are likely to be located outside of this 1 kb fragment.
Renaudie
F
F
INSERM, U. 409, Faculté Xavier-Bichat, Paris, France.
Boulanger
L
L
Grandchamp
B
B
Beaumont
C
C
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005293:Ferritins / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic
D012333:RNA, Messenger; D005293:Ferritins
Clonage, caractérisation et expression du gène de la sous-unité L ferritine de souris.