Expression of syntaxin 4 in rat skeletal muscle and rat skeletal muscle cells in culture.
Syntaxins are a family of membrane proteins believed to participate in docking/fusing of arriving vesicles during membrane sorting and secretion. Of the six mammalian syntaxins known, only brain syntaxin 1A/1B has been biochemically characterized in its endogenous form. Syntaxin 4 mRNA is expressed in selective tissues including rat skeletal muscle, although it has not been studied at the protein level in any cell type. Therefore, we generated an affinity-purified antibody against syntaxin 4 to demonstrate that this 36 kDa protein is expressed in rat skeletal muscle and L6 muscle cells in culture. The content of the syntaxin 4 protein increased by 1.9-fold during differentiation of L6 myoblasts into myotubes. By subcellular fractionation the protein was mainly recovered in plasma membrane-enriched fractions of both red and white skeletal muscles and L6 myotubes. Coupled to the recent detection of vesicle associated membrane protein-2 and cellubrevin in skeletal muscle, syntaxin 4 may play a role in membrane traffic in this tissue.
Sumitani
S
S
Division of Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada.
Ramlal
T
T
Liu
Z
Z
Klip
A
A
D000818:Animals; D002454:Cell Differentiation; D002458:Cell Fractionation; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D015870:Gene Expression; D008565:Membrane Proteins / Q000032:analysis* / Q000235:genetics; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000648:ultrastructure; D050765:Qa-SNARE Proteins; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D050827:Syntaxin 1
D008565:Membrane Proteins; D050765:Qa-SNARE Proteins; D012333:RNA, Messenger; C494213:Stx1a protein, rat; D050827:Syntaxin 1