Cloning and sequencing of a cDNA encoding a novel member of the human brain GABA/noradrenaline neurotransmitter transporter family.
Screening of a human hippocampal cDNA library with a rat creatine transporter cDNA-specific probe revealed two types of clones. One identical to the published creatine transporter cDNA sequence (CRT1) and another (CRT2) with four segments of oligodeoxyribonucleotide substitution or addition in different sites of the CRT1 coding sequence. Translation of the CRT2 coding sequence reveals a new protein with regions of perfect homology with the CRT1 amino-acid sequence.
Barnwell
L F
LF
Department of Physiology, Meharry Medical College, Nashville, TN 37208, USA.
Chaudhuri
G
G
Townsel
J G
JG
D000595:Amino Acid Sequence; D001483:Base Sequence; D001923:Brain Chemistry / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D050485:GABA Plasma Membrane Transport Proteins; D006624:Hippocampus; D006801:Humans; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D018377:Neurotransmitter Agents / Q000378:metabolism; D050484:Norepinephrine Plasma Membrane Transport Proteins; D027361:Organic Anion Transporters; D050480:Plasma Membrane Neurotransmitter Transport Proteins; D017422:Sequence Analysis, DNA; D027981:Symporters
D002352:Carrier Proteins; D018076:DNA, Complementary; D050485:GABA Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; D050484:Norepinephrine Plasma Membrane Transport Proteins; D027361:Organic Anion Transporters; D050480:Plasma Membrane Neurotransmitter Transport Proteins; C493227:SLC6A2 protein, human; C094770:SLC6A8 protein, human; D027981:Symporters; C065637:choline transporter; C080679:creatine transporter