Characterization of underivatized tetrapeptides by negative-ion fast-atom bombardment mass spectrometry.
The [M-H]- ions derived from tetrapeptides generally show two different collision-induced backbone cleavages which allow the determination of the amino acid sequence of the peptide. The first of these involves the formation of the carboxylate anions of either constituent amino acids or fragment peptides. In the second, amino acids or fragment peptides are eliminated as neutrals. There are a number of residues which undergo characteristic side-chain fragmentations irrespective of their position in the tetrapeptide, e.g. Ser, Thr, Cys, Met, Phe, and Tyr. However, there are also some residues which, when situated at the C-terminal end of the peptide, promote pronounced fragmentation at the C-terminal position which occurs to the exclusion of the normal backbone cleavages. We conclude that the data obtained from these negative-ion cleavages are analytically useful, and complement those provided by the cognate positive-ion technique.
Bradford
A M
AM
Department of Chemistry, University of Adelaide, South Australia.
Waugh
R J
RJ
Bowie
J H
JH
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000596:Amino Acids; D009842:Oligopeptides