Selective disulphide linkage of plant thionins with other proteins.
Thionins are shown to form disulphide linkages with other proteins. The reaction with bacterial enzymes beta-glucuronidase and neomycin phosphotransferase II could be prevented and reversed with dithiothreitol and blocked with N-ethylmaleimide. Other cysteine-rich low-molecular-weight toxic peptides from plants (LTP-3 from barley and P19 from potato) did not react as the thionins. Certain cysteine-containing proteins, such bovine serum albumin, ovalbumin and cytochrome c, reacted with thionins, while others, including carbonic anhydrase, soybean trypsin inhibitor, bovine-lung trypsin inhibitor and phosphorylase B did not. Selectivity of the reaction with a periplasmic component of the phytopathogenic bacterium Pseudomonas solanacearum was also shown.
Piñeiro
M
M
Departamento de Biotecnología, ETS Ingenieros Agrónomos - UPM, Madrid, Spain.
Diaz
I
I
Rodriguez-Palenzuela
P
P
Titarenko
E
E
Garcia-Olmedo
F
F
D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins / Q000737:chemistry; D003545:Cysteine / Q000037:antagonists & inhibitors / Q000737:chemistry*; D003574:Cytochrome c Group / Q000737:chemistry; D004220:Disulfides / Q000037:antagonists & inhibitors / Q000737:chemistry*; D004229:Dithiothreitol / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D005966:Glucuronidase / Q000037:antagonists & inhibitors / Q000737:chemistry; D005978:Glutathione / Q000031:analogs & derivatives / Q000494:pharmacology; D019803:Glutathione Disulfide; D019868:Kanamycin Kinase; D010047:Ovalbumin / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D011549:Pseudomonas / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014118:Toxins, Biological / Q000737:chemistry
D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins; D003574:Cytochrome c Group; D004220:Disulfides; D010455:Peptides; D010940:Plant Proteins; D011506:Proteins; D014118:Toxins, Biological; C074252:TthV protein, wheat; D012710:Serum Albumin, Bovine; D010047:Ovalbumin; D017853:Phosphotransferases (Alcohol Group Acceptor); D019868:Kanamycin Kinase; D005966:Glucuronidase; D005978:Glutathione; D003545:Cysteine; D005033:Ethylmaleimide; D004229:Dithiothreitol; D019803:Glutathione Disulfide