Hypochlorite scavenging activity of polyphenols.
Chemiluminescence, generated by the mixture of sodium hypochlorite solution and luminol, was completely eliminated by polyphenols, such as natural lignins, phenylpropenoid monomers and polymers, and epigallocatechin gallate. On the other hand, hypochlorite scavenging activity of polysaccharides, such as PSK (Krestin) and Schizophyllan, was relatively weak. Human myelogenous leukemic cell lines (HL-60, ML-1) showed higher production of active oxygen(s) (detected by luminol chemiluminescence) and iodination capacity, than six other cultured cell lines. Since lignin did not completely eliminate the active oxygen production by HL-60 cells, possible stimulation of hypochlorite production by lignin was suggested.
Sakagami
H
H
First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.
Sakagami
T
T
Yoshida
H
H
Omata
T
T
Shiota
F
F
Takahashi
H
H
Kawazoe
Y
Y
Takeda
M
M
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology; D002460:Cell Line; D003373:Coumaric Acids / Q000494:pharmacology; D005419:Flavonoids; D016166:Free Radical Scavengers; D005909:Glioblastoma; D006801:Humans; D006997:Hypochlorous Acid; D007454:Iodides; D007938:Leukemia; D007951:Leukemia, Myeloid; D008031:Lignin / Q000494:pharmacology; D008163:Luminescent Measurements; D008165:Luminol; D010636:Phenols / Q000494:pharmacology*; D011108:Polymers / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism*; D012867:Skin; D014197:Trees; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D003373:Coumaric Acids; D005419:Flavonoids; D016166:Free Radical Scavengers; D007454:Iodides; D010636:Phenols; D011108:Polymers; D017382:Reactive Oxygen Species; D008165:Luminol; D006997:Hypochlorous Acid; D002392:Catechin; D008031:Lignin; C004999:ferulic acid; C045651:epigallocatechin gallate; C010559:coniferyl alcohol