Selection and partial characterization of calcium ionophore (A23187) resistant cells.
We have selected calcium ionophore (A23187)-resistant cells (AR-7) in a human promyelocytic leukemia cell line, HL-60. AR-7 showed approximately 8.5-fold resistance to A23187 compared with the wild type cells after continuous exposure for 3 days. AR-7 had cross resistance to ionomycin (4.6-fold) and thapsigargin (340-fold) which can also increase intracellular Ca++. Similar magnitude of resistance to apoptosis, as defined by characteristic morphology and internucleosomal DNA fragmentation, induced by these agents was observed after 4 hr of incubation. However, both the elevation of intracellular Ca++ following stimulation with various concentrations of A23187 and the sensitivity to anti-cancer agents including etoposide, 1-beta-D arabinofuranosylcytosine, and hydroxyurea were comparable between the two cell types. This cell line is considered to be useful for exploring the mechanism(s) of cell death, especially apoptosis, induced by calcium ionophore.
Kubota
M
M
Department of Pediatrics, Faculty of Medicine, Kyoto University, Japan.
Kataoka
A
A
Okuda
A
A
Bessho
R
R
Lin
Y W
YW
Wakazono
Y
Y
Usami
I
I
Akiyama
Y
Y
Furusho
K
K
D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002455:Cell Division; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D004351:Drug Resistance; D005047:Etoposide / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D015759:Ionomycin / Q000494:pharmacology; D015473:Leukemia, Promyelocytic, Acute; D013729:Terpenes / Q000494:pharmacology; D019284:Thapsigargin; D014407:Tumor Cells, Cultured; D014466:Ultraviolet Rays
D000970:Antineoplastic Agents; D013729:Terpenes; D003561:Cytarabine; D000001:Calcimycin; D015759:Ionomycin; D019284:Thapsigargin; D005047:Etoposide; D004247:DNA; D002118:Calcium