title: Rapid gas chromatographic screening of edible seeds, nuts and beans for non-protein and protein amino acids.
abstract: Water extraction with subsequent picric acid treatment and solvent washing (ethyl acetate and diethyl ether) was employed for the rapid isolation of free amino acids from non-aqueous food samples. The isolated amino acids were subjected to N(O,S)-isobutyloxycarbonylation followed by solid-phase extraction and tert.-butyldimethylsilylation for direct analysis by gas chromatography and gas chromatography-mass spectrometry. When the present method was applied to nineteen food samples (common beans, seeds, and nuts), seventeen protein amino acids and fifteen non-protein amino acids were simultaneously screened. Eleven non-protein amino acids including gamma-aminobutyric acid, pipecolic acid, pyroglutamic acid, alpha-aminobutyric acid, alpha-aminoadipic acid, and beta-alanine were tentatively identified, and four compounds assumed to be non-protein amino acids remained unidentified.
authors.affiliation: Department of Food and Biotechnology, Yonsei University, Seoul, South Korea.
authors.forename: C H
authors.initials: CH
authors.lastname: Oh
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: K R
authors.initials: KR
authors.lastname: Kim
authors.forename: T J
authors.initials: TJ
authors.lastname: Mabry
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D007887:Fabaceae / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D009754:Nuts / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry*; D010945:Plants, Edible / Q000737:chemistry*; D010946:Plants, Medicinal; D012639:Seeds / Q000737:chemistry; D014118:Toxins, Biological / Q000032:analysis
chemical_list: D000596:Amino Acids; D010940:Plant Proteins; D014118:Toxins, Biological