title: Escherichia coli JA221 can suppress the UAG stop signal.
abstract: The Escherichia coli strain JA221 can suppress the UAG stop codon, although the existence of an amber suppressor tRNA has not previously been described for this strain. When using a plasmid to express alpha-sarcin, which has TAG as its stop signal, two proteins were obtained: a smaller protein corresponding in size to that of the expected protein, and a larger protein, which could be accounted for by the presence of a second stop codon (TGA) 18 base pairs downstream of the original. This feature of strain JA221 must therefore be considered when using this strain as a host for the production of recombinant proteins.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense, Madrid, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Lacadena
authors.forename: A
authors.initials: A
authors.lastname: Martínez del Pozo
authors.forename: J M
authors.initials: JM
authors.lastname: Mancheño
authors.forename: M
authors.initials: M
authors.lastname: Gasset
authors.forename: M
authors.initials: M
authors.lastname: Oñaderra
authors.forename: J G
authors.initials: JG
authors.lastname: Gavilanes
mesh_terms: D018388:Codon, Terminator / Q000235:genetics*; D004722:Endoribonucleases; D004926:Escherichia coli / Q000235:genetics*; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D013489:Suppression, Genetic / Q000502:physiology*
chemical_list: D018388:Codon, Terminator; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; C014801:alpha-sarcin; D012343:RNA, Transfer; D004722:Endoribonucleases