Protective role of chronic ubiquinone administration on acute cardiac oxidative stress.
Previous studies have shown that acute exogenous administration of coenzyme ubiquinone (CoQ10) can protect the heart against oxidant-mediated injury. The aim of this study was to investigate whether protection against cardiac oxidative stress could be obtained by increasing tissue levels of CoQ10, as achieved by chronic CoQ10 supplementation. Wistar rats were randomly divided into two groups: a control group given standard diet and a test group receiving diet supplemented with CoQ10 (5 mg/kg/day) for 4 weeks. Functional and metabolic changes induced by oxidative stress were investigated in isolated perfused hearts and in papillary muscles. Tissue concentrations of ubiquinones were significantly higher in the left ventricle of treated rats than in controls. H2O2 infusion (60 microM for 60 min) induced marked alterations of both developed pressure, which decreased to -58.8 +/- 16.8% of base line and end-diastolic pressure which increased almost 13-fold. These effects were reduced significantly (P < .05) in hearts from CoQ10-supplemented rats (-13.8 +/- 2.3 and +375.0 +/- 42.5%, respectively). In the same hearts, cumulative release of oxidized glutathione (a specific marker of oxidative stress) was 450.2 +/- 69.2 nmol/g of wet weight in the control group and only 89.6 +/- 22.3 nmol/g of wet weight in treated hearts (P < .01). In papillary muscles, after 60 min of perfusion with H2O2, active tension decreased, largely in controls whereas it was almost unchanged in the treated group (-34.4 +/- 7.5% of baseline vs. -0.1 +/- 0.05%, P < .05).(ABSTRACT TRUNCATED AT 250 WORDS)
Ferrara
N
N
Istituto di Medicina Interna, Università degli Studi di Napoli, Federico II, Italy.
Abete
P
P
Ambrosio
G
G
Landino
P
P
Caccese
P
P
Cirillo
P
P
Oradei
A
A
Littarru
G P
GP
Chiariello
M
M
Rengo
F
F
D000818:Animals; D006321:Heart / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000633:toxicity; D008297:Male; D009206:Myocardium / Q000378:metabolism; D018384:Oxidative Stress; D010210:Papillary Muscles / Q000187:drug effects / Q000378:metabolism; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D014451:Ubiquinone / Q000493:pharmacokinetics / Q000494:pharmacology*
D014451:Ubiquinone; D006861:Hydrogen Peroxide