title: NAD(+)-glycohydrolase from Streptococcus pyogenes shows cyclic ADP-ribose forming activity.
abstract: NAD(+)-glycohydrolase from Streptococcus pyogenes was purified by successive chromatography on CM Sepharose CL-6B, Sephacryl S-200 HR and hydroxyapatite. The purified enzyme possessed synthesis and hydrolysis activities of cyclic ADP-ribose (cADPR), a newly found second messenger for Ca2+ mobilisation, along with cleavage activity of the ribose-nicotinamide bond in NAD+.
authors.affiliation: Department of Bacteriology, School of Medicine, Kanazawa University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Karasawa
authors.forename: S
authors.initials: S
authors.lastname: Takasawa
authors.forename: K
authors.initials: K
authors.lastname: Yamakawa
authors.forename: H
authors.initials: H
authors.lastname: Yonekura
authors.forename: H
authors.initials: H
authors.lastname: Okamoto
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000031:analogs & derivatives* / Q000096:biosynthesis; D036563:Cyclic ADP-Ribose; D009244:NAD+ Nucleosidase / Q000302:isolation & purification / Q000494:pharmacology*; D013297:Streptococcus pyogenes / Q000201:enzymology*
chemical_list: D036563:Cyclic ADP-Ribose; D000246:Adenosine Diphosphate Ribose; D009244:NAD+ Nucleosidase