Cloning, sequencing and expression of an isoform of cardiac C-protein from the Mexican axolotl (Ambystoma mexicanum).
C-protein, a myosin binding protein, is thought to regulate and stabilize thick filaments during assembly of sarcomeric A-bands. Multiple isoforms of C-protein have been characterized in avian and mammalian systems. We now report the isolation and the nucleic acid sequence of a partial C-protein cDNA clone from an axolotl heart cDNA expression library in lambda gt11. The clone was isolated by screening the library with a heterologous monoclonal anti-C-protein antibody (MF1). Sequence comparison revealed that CPROAxocard1 has an average sequence identity of 62-68% at the nuclei acid and 72-78% at the amino acid levels respectively to human and chicken sequences. We could not detect any significant differences at the levels of expression of the cardiac isoform of C-protein (CPROAxocard1) in normal and non-beating heart tissues of the double-recessive cardiac lethal mutant (c/c) axolotl, Ambystoma mexicanum. This is the first report of a C-protein sequence from an amphibian species.
Ward
S M
SM
SUNY Health Science Center at Syracuse, Department of Anatomy and Cell Biology 13210, USA.
Lemanski
L F
LF
Erginel-Unaltuna
N
N
Dube
D K
DK
Eiginel-Unaltuna
N
N
D000558:Ambystoma mexicanum; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D015870:Gene Expression; D015723:Gene Library; D005804:Genes, Lethal; D005808:Genes, Recessive; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism; D009928:Organ Specificity; D016133:Polymerase Chain Reaction; D000099163:Myosin Binding Protein C
D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D009218:Myosins; D000099163:Myosin Binding Protein C