An S-RNase promoter from Nicotiana alata functions in transgenic N. alata plants but not Nicotiana tabacum.
Nicotiana tabacum and Nicotiana alata plants were transformed with genomic clones of two S-RNase alleles from N. alata. Neither the S2 clone, with 1.6 kb of 5' sequence, nor the S6 clone, with 2.8 kb of 5' sequence, were expressed at detectable levels in transgenic N. tabacum plants. In N. alata, expression of the S2 clone was not detected, however the S6 clone was expressed (at low levels) in three out of four transgenic plants. An S6-promoter-GUS fusion gene was also expressed in transgenic N. alata but not N. tabacum. Although endogenous S-RNase genes are expressed exclusively in floral pistils, the GUS fusion was expressed in both styles and leaves.
Murfett
J
J
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia.
Ebert
P R
PR
Haring
V
V
Clarke
A E
AE
D003001:Cloning, Molecular; D005091:Exons; D018506:Gene Expression Regulation, Plant; D018515:Plant Leaves / Q000235:genetics; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012260:Ribonucleases / Q000235:genetics*; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*; D014170:Transformation, Genetic
D011993:Recombinant Fusion Proteins; D012260:Ribonucleases; C022542:ribonuclease S