[Demonstration of thermostable enzymes in thermophilic micro-organisms of hydrothermal origin].
During 3 cruises in the Pacific ocean, hydrothermal samples have been collected and some thermophilic bacteria and archaea have been purified. Four enzymatic activities have been screened on 77 chemo-organoheterotrophic thermophilic microorganisms. Forty-two isolates exhibited intracellular beta-glucosidase activity whereas only 7 (including only one archaeon) showed alcohol dehydrogenase one. Protease activity was not detected on only 6 isolates over 77. Twenty-seven isolates exhibited esterase activity and 3 different electrophoretic patterns have been revealed. No isolate was found to exhibit the 4 activities. Preliminary characterization of these activities showed high thermophily and thermostability, properties which could be used in potential biotechnological applications.
Ladrat
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C
IFREMER, Laboratoire de biotechnologie, Plouzané, France.
Cornec
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L
Alayse-Danet
A M
AM
Barbier
G
G
D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D010450:Endopeptidases / Q000378:metabolism*; D004795:Enzyme Stability; D004950:Esterases / Q000378:metabolism*; D006358:Hot Temperature; D012623:Seawater; D014871:Water Microbiology; D001617:beta-Glucosidase / Q000378:metabolism*
D000426:Alcohol Dehydrogenase; D004950:Esterases; D001617:beta-Glucosidase; D010450:Endopeptidases
Mise en évidence d'enzymes thermostables chez des micro-organismes thermophiles d'origine hydrothermale.