Expression of the erythrocyte antigen Henshaw (He; MNS6): serological and immunochemical studies.
Production of murine monoclonal antibodies to the low prevalence MNS antigen Henshaw (He; MNS6) has enabled more detailed study of this antigen. Using these directly hemagglutinating anti-He, red blood cells (RBCs) from 1695 people of African origin were screened in the USA and England. The prevalence of He+ samples among these donors was 2.1%. In Natal, blood samples from 1218 black donors were screened with rabbit anti-He. The prevalence of He+ donors in this population was 7.0%. Immunoblotting confirmed that the He antigen is carried on an erythrocyte membrane component with a molecular mass that is indistinguishable from glycophorin B. Hemagglutination and immunoblotting demonstrated that ten of 56 He+ samples tested more extensively had a reduced expression of the He antigen. The majority of He+ RBCs were S+; those He+ RBC samples that were S-s+ more frequently had a weakened expression of He.
Reid
M E
ME
Immunohematology Laboratory, New York Blood Center, N.Y. 10021, USA.
Lomas-Francis
C
C
Daniels
G L
GL
Chen
V
V
Shen
J
J
Ho
Y C
YC
Hare
V
V
Batts
R
R
Yacob
M
M
Smart
E
E
D000911:Antibodies, Monoclonal; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007519:Isoantigens / Q000097:blood*; D008951:MNSs Blood-Group System / Q000276:immunology*; D012698:Serologic Tests
D000911:Antibodies, Monoclonal; D007519:Isoantigens; D008951:MNSs Blood-Group System