Growth of Serpulina (Treponema) hyodysenteriae under iron-restricted conditions.
Reference strains of Serpulina hyodysenteriae expressed at least three iron-regulated proteins with apparent molecular masses of > 200, 134, and 109 kDa when grown under iron-restricted conditions. Cells of S. hyodysenteriae grown under these conditions also showed increased outer membrane bleb formation when examined by electron microscopy after negative staining. S. hyodysenteriae did not use the 2 most common types of siderophore, namely catechol and hydroxamate. Western blotting with serum from a pig experimentally infected with S. hyodysenteriae B204 indicated that the 109-kDa major iron-regulated protein was expressed in vivo and was conserved among all strains tested.
Li
Z
Z
Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec.
Foiry
B
B
Jacques
M
M
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D001426:Bacterial Proteins; D017401:Brachyspira hyodysenteriae / Q000254:growth & development* / Q000378:metabolism; D004586:Electrophoresis / Q000662:veterinary; D007501:Iron / Q000378:metabolism*; D033862:Iron-Binding Proteins; D008854:Microscopy, Electron / Q000662:veterinary; D033902:Periplasmic Binding Proteins; D017262:Siderophores / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D033862:Iron-Binding Proteins; D033902:Periplasmic Binding Proteins; D017262:Siderophores; D007501:Iron