Interleukin-1 beta induces the synthesis of adenylyl cyclase in Swiss 3T3 fibroblasts and MG-63 osteosarcoma cells.
In this study, the longer term (> or = 6 hours) effect of interleukin-1 beta on adenylyl cyclase activity was investigated in Swiss 3T3 and MG-63 cells. No change was evident after 6 hours but after 1, 7 or 15 day incubations a significant increase in basal (1.5- 2 fold) and NaF-stimulated (2-4 fold) adenylyl cyclase activity was observed in interleukin-1 beta pre-treated cell membranes compared with non pre-treated controls. The response to forskolin, a direct stimulus of adenylyl cyclase, was also significantly enhanced, indicating that the effect of interleukin-1 beta was targeted to the enzyme itself. This action of interleukin-1 beta was blocked by co-incubation with cycloheximide, an inhibitor of protein synthesis, which demonstrated that de novo protein synthesis was required. It is concluded that interleukin-1 induces the expression of adenylyl cyclase in 3T3 and MG-63 cells, leading to enhanced activation by NaF and forskolin.
Armour
K J
KJ
Department of Human Metabolism, University of Sheffield Medical School, UK.
Smith
N W
NW
Brown
B L
BL
Dobson
P R
PR
D016475:3T3 Cells / Q000201:enzymology*; D000262:Adenylyl Cyclases / Q000096:biosynthesis*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D005576:Colforsin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology*; D051379:Mice; D012516:Osteosarcoma / Q000201:enzymology*; D012969:Sodium Fluoride / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D007375:Interleukin-1; D005576:Colforsin; D012969:Sodium Fluoride; D003513:Cycloheximide; D000262:Adenylyl Cyclases