Isolation of microfilariae and eggs of Setaria digitata.
A simple procedure of gel-filtration was used to purify microfilariae of the cattle parasite, Setaria digitata from its eggs. The eggs of S. digitata were also isolated from the female worms by picking out the uterine canal. Gel-filtration gave a recovery of 70% for microfilariae.
Das
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Division of Parasite Immunology, Regional Medical Research Centre (ICMR), Bhubaneswar, India.
Das
M K
MK
D000818:Animals; D002417:Cattle; D002469:Cell Separation / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D005260:Female; D009865:Oocytes / Q000166:cytology*; D017182:Setaria Nematode / Q000302:isolation & purification* / Q000502:physiology*; D014599:Uterus / Q000469:parasitology