title: Induction of protective immunity in chickens orally immunized with inactivated infectious bursal disease virus.
abstract: The present studies were undertaken to examine the effects of oral administration of formalin-inactivated infectious bursal disease virus (IBDV) on the immune responses of chickens. Inactivated IBDV was suspended in phosphate-buffered saline containing sodium bicarbonate. This form of antigen, when administered by oral instillation, induced a serum antibody response against IBDV in chickens and these chickens were protected from subsequent viral challenge. The immunoglobulin class of IBDV-specific antibodies in serum was found to be IgG when determined by enzyme-linked immunosorbent assay. Cholera toxin, which has been reported to have potent mucosal adjuvant properties in mammals, did not enhance the serum antibody response. Oral followed by parenteral administrations of antigen induced an enhanced antibody response in chickens.
authors.affiliation: Nippon Institute for Biological Science, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Hoshi
authors.forename: T
authors.initials: T
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Nunoya
authors.forename: S
authors.initials: S
authors.lastname: Ueda
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D000956:Antigens, Viral / Q000494:pharmacology; D018175:Birnaviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D002645:Chickens; D002772:Cholera Toxin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006385:Hemagglutination Inhibition Tests; D007113:Immunity, Innate; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007243:Infectious bursal disease virus / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control*; D015164:Vaccines, Inactivated / Q000627:therapeutic use*; D014765:Viral Vaccines / Q000627:therapeutic use*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D002772:Cholera Toxin