Modification of an HLA-B PCR-SSOP typing system leading to improved allele determination.
Modifications have been introduced to a previously reported HLA-B PCR-SSOP typing system. This has enabled further definition of alleles, determination of the probe pattern of some alleles not previously examined and identification of patterns of possible new alleles. However there are still some alleles that cannot be differentiated and there are several alleles which when present as a homozygote have the same pattern as in combination with another allele. When the method was applied to the typing of 66 consecutive cadaveric donors there were three donors whose type differed from the serological type.
Middleton
D
D
Northern Ireland Tissue Typing Laboratory, City Hospital, Belfast, Northern Ireland, United Kingdom.
Williams
F
F
Cullen
C
C
Mallon
E
E
D000483:Alleles; D001483:Base Sequence; D002460:Cell Line; D015343:DNA Probes, HLA; D015235:HLA-B Antigens / Q000145:classification / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*
D015343:DNA Probes, HLA; D015235:HLA-B Antigens; D015345:Oligonucleotide Probes