N-proximal sequence motif in light-harvesting chlorophyll a/b-binding protein is essential for the trimerization of light-harvesting chlorophyll a/b complex.
The major light-harvesting complex (LHCII) of photosystem II can be reconstituted in its native, trimeric form starting from its apoprotein light-harvesting chlorophyll a/b-binding protein (LHCP), pigments, and thylakoid lipids. In this paper we identify segments in the LHCP polypeptide that are essential for the formation of stable LHCII trimers by analyzing N- and C-terminal deletion mutants of LHCP and mutants carrying point-specific amino acid exchanges. C-terminal deletions that do not abolish pigment binding to LHCP do not affect trimerization either. By contrast, on the N-terminus of LHCP, where as many as 61 amino acids can be deleted without significant effects on pigment binding, only 15 amino acids are dispensible for LHCII trimer formation. This indicates that structural elements between amino acids 16 and 61 are involved in the stabilization of LHCII trimers but not monomers. Closer inspection of this protein domain in a more detailed mutation analysis revealed that amino acids W16 and/or Y17 as well as R21 are essential for the formation of LHCII trimers. These amino acids are conserved in virtually all known sequences of LHCII apoproteins but only in some of the minor chlorophyll a/b complexes. Possible functions of the crucial residues are discussed.
Hobe
S
S
Botanisches Institut III der Universität, München, Germany.
Förster
R
R
Klingler
J
J
Paulsen
H
H
D000595:Amino Acid Sequence; D001483:Base Sequence; D001704:Biopolymers; D017931:DNA Primers; D004591:Electrophoresis, Polyacrylamide Gel; D045342:Light-Harvesting Protein Complexes; D008969:Molecular Sequence Data; D016296:Mutagenesis; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000235:genetics; D045332:Photosystem II Protein Complex; D011489:Protein Denaturation
D001704:Biopolymers; D017931:DNA Primers; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex