Purification and characterization of a factor which inhibits retrovirus replication.
Chick embryo cells (CEC) secrete a factor which inhibits HIV1 replication. Non-productive infection of CEC with vesicular stomatitis virus increases 5-10-fold the synthesis of this factor. After purification by FPLC the biological activity is associated with a heat-resistant protein of approximately 150 kDa, composed of two subunits of 70 and 58 kDa and charged negatively. Monoclonal antibodies raised against this protein block the inhibitory activity of the purified protein and react with the 150-kDa and 58-kDa proteins in Western blots.
Krief
P
P
INSERM Unité 268, Villejuif, France.
Lawrence
D
D
Alileche
A
A
Plaisance
S
S
Jasmin
C
C
Semmel
M
M
D000818:Animals; D000998:Antiviral Agents / Q000032:analysis* / Q000494:pharmacology; D002642:Chick Embryo; D017077:Culture Media, Conditioned; D015497:HIV-1 / Q000187:drug effects* / Q000276:immunology / Q000502:physiology; D011506:Proteins / Q000032:analysis* / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D017077:Culture Media, Conditioned; D011506:Proteins