Infectious cDNA clones of echovirus 12 and a variant resistant against the uncoating inhibitor rhodanine differ in seven amino acids.
Determination of the complete sequences of echovirus 12 and a rhodanine-resistant variant revealed seven amino acid deviations and two additional exchanges not confirmed in all clones. In rhodanine sensitivity assays with infectious cDNAs, it was shown that the biological markers of the original viruses are maintained.
Kraus
W
W
Institut für Virologie, Universität zu Köln, Germany.
Zimmermann
H
H
Zimmermann
A
A
Eggers
H J
HJ
Nelsen-Salz
B
B
D000595:Amino Acid Sequence; D000998:Antiviral Agents / Q000494:pharmacology*; D001483:Base Sequence; D002213:Capsid / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004352:Drug Resistance, Microbial / Q000235:genetics; D029822:Enterovirus B, Human / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D014644:Genetic Variation; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012235:Rhodamines / Q000494:pharmacology*; D014158:Transcription, Genetic
D000998:Antiviral Agents; D018076:DNA, Complementary; D011994:Recombinant Proteins; D012235:Rhodamines