title: Tn5384, a composite enterococcal mobile element conferring resistance to erythromycin and gentamicin whose ends are directly repeated copies of IS256.
abstract: We have identified a 26-kb mobile element from Enterococcus faecalis CH116, designated Tn5384, which confers resistance to erythromycin and to high levels of gentamicin. Tn5384 is a composite element containing three copies of insertion element IS256. Two of the IS256 copies flank the aac6'-aph2" bifunctional aminoglycoside-modifying-enzyme gene in the inverted orientation, forming a structure similar to staphylococcal gentamicin resistance transposon Tn4001. One of the IS256 elements involved in the Tn4001-like structure also forms the left end of Tn5384, the right end of which is a directly repeated insertion of IS256 approximately 23 kb downstream of the leftmost insertion. Insertions of Tn5384 into enterococcal plasmid pLRM1 have been found associated with 8- and 9-bp duplications of the target sequence.
authors.affiliation: Medical Research Service, Department of Veterans Affairs Medical Center, Cleveland, Ohio 44106, USA.
authors.forename: L B
authors.initials: LB
authors.lastname: Rice
authors.forename: L L
authors.initials: LL
authors.lastname: Carias
authors.forename: S H
authors.initials: SH
authors.lastname: Marshall
mesh_terms: D001483:Base Sequence; D003227:Conjugation, Genetic; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial / Q000235:genetics; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics*; D004917:Erythromycin / Q000494:pharmacology*; D005839:Gentamicins / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D005839:Gentamicins; D004917:Erythromycin