Influence of synthetic peptides, corresponding to fragments of the human alpha-2 interferon molecule, on the proliferation of lymphoblastoid cells in vitro. Growth inhibition and receptor binding.
Influence of nine synthetic peptides from the C-terminal part of human alpha-2 interferon (IFN) molecule on growth of a human T-lymphoblastoid cell line MT-4 was investigated. It was shown that some peptides inhibited the proliferation of MT-4 cells. It was also found that MT-4 cells expressed specific receptors on the outer surface of the plasma membrane. All studied peptides competed for binding to a common binding site. Antiproliferative activity of the peptides correlated with their length and affinity to receptors on the outer membrane of MT-4 cells.
Danilkovich
A
A
Department of Cellular Physiology and Immunology, Biological Faculty, Moscow State University, Russian Federation.
Freze
K
K
Romashkova
J
J
Valujskikh
A
A
Makarov
E
E
Targoni
O
O
Makarova
N
N
Kushch
A
A
D000595:Amino Acid Sequence; D001667:Binding, Competitive; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006801:Humans; D016898:Interferon-alpha / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D017471:Receptors, Interferon / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology*
D016898:Interferon-alpha; D010446:Peptide Fragments; D017471:Receptors, Interferon