[Milrinone suppresses TNF-alpha and IL-1 beta release in mouse peritoneal macrophages].
Tumor necrosis factor-alpha (TNF-alpha) exerts a wide spectrum of biological activities and contributes to the pathophysiology of septic shock. We studied whether milrinone suppresses TNF-alpha and IL-1 beta releases from mouse peritoneal macrophages. Mouse peritoneal macrophages were stimulated for 18 hr with lipopolysaccharide and different doses of milrinone. TNF-alpha release was suppressed in a dose-dependent fashion with milrinone, reaching half-maximal inhibition at 30 microM. Release of IL-1 beta was not suppressed with 25 microM of milrinone, but it was suppressed with 250 microM of milrinone. We conclude that TNF-alpha release is suppressed by therapeutically administered milrinone.
Masaki
Y
Y
Department of Anesthesiology, Akita University School of Medicine.
Sato
W
W
Kayaba
M
M
Enzan
K
K
Suzuki
M
M
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D007375:Interleukin-1 / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D020105:Milrinone; D011728:Pyridones / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D007375:Interleukin-1; D011728:Pyridones; D014409:Tumor Necrosis Factor-alpha; D020105:Milrinone