title: Functional linkage between the active site of alpha-lytic protease and distant regions of structure: scanning alanine mutagenesis of a surface loop affects activity and substrate specificity.
abstract: Previous structural and kinetic characterization of mutations within the active site of alpha-lytic protease have demonstrated that amino acid residues in direct contact with the substrate are major substrate specificity determinants. The experiments described here identify residues 216-226 of alpha-lytic protease as a region of structure peripheral to the active site that also plays an important role in establishing the substrate specificity of the enzyme. Alanine substitution mutations within this surface loop of 19 amino acid residues significantly perturb the enzyme's specificity profile, despite being as far as 21 A from the hydroxyl group of Ser195. The kinetic consequences of the mutations are remarkably independent of position within the loop and suggest that active site plasticity is affected more than static structure. Kinetic characterization of double mutants with the Met190-->Ala broad-specificity active site mutation reveals varying degrees of non-additivity and indicates that active site plasticity can be influenced through multiple sets of interactions. Although these results clearly demonstrate that tuning of serine protease activity is possible through remodelling of structure surrounding the active site, practical issues such as retaining compatibility with the folding mechanism and stability of the mature enzyme present significant obstacles to general application of the technique.
authors.affiliation: Howard Hughes Medical Institute, University of California, San Francisco 94143-0448, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Mace
authors.forename: B J
authors.initials: BJ
authors.lastname: Wilk
authors.forename: D A
authors.initials: DA
authors.lastname: Agard
mesh_terms: D000409:Alanine / Q000235:genetics*; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry; D001665:Binding Sites; D002918:Chymotrypsin / Q000737:chemistry; D010450:Endopeptidases / Q000737:chemistry; D007700:Kinetics; D015394:Molecular Structure; D016296:Mutagenesis; D009154:Mutation; D010196:Pancreatic Elastase / Q000737:chemistry; D011487:Protein Conformation; D015202:Protein Engineering; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism*; D013305:Streptomyces griseus / Q000201:enzymology; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014357:Trypsin / Q000737:chemistry
chemical_list: D010450:Endopeptidases; D012697:Serine Endopeptidases; D002918:Chymotrypsin; C021826:myxobacter alpha-lytic proteinase; D010196:Pancreatic Elastase; D014357:Trypsin; C022325:aspartic proteinase A; D016282:Aspartic Acid Endopeptidases; D000409:Alanine