Separation of hydroxyl protected heparin derived disaccharides using reversed-phase high-performance liquid chromatography.
A simple and efficient method for the separation of hydrophobic derivatives of glycosaminoglycan-derived disaccharides is described. Hydroxyl-protected derivatives of a trisulfated disaccharide, prepared from heparin using heparin lyase, were separated by reversed-phase high-performance liquid chromatography. These disaccharide derivatives differed by the number, position, and stereochemistry of acetyl and pivaloyl groups. Separation was achieved on a C18 column using a reversed gradient of ammonium sulfate in water. This method has application in the purification of disaccharide derivatives being used as chiral synthons in the preparation of higher oligosaccharides.
Kerns
R J
RJ
Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa City 52242, USA.
Linhardt
R J
RJ
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004187:Disaccharides / Q000737:chemistry / Q000302:isolation & purification*; D006493:Heparin / Q000737:chemistry*; D008969:Molecular Sequence Data
D004187:Disaccharides; D006493:Heparin