title: Isolation of black creek canal virus, a new hantavirus from Sigmodon hispidus in Florida.
abstract: Numerous rodents were trapped for serologic and virologic studies following the identification of a hantavirus pulmonary syndrome (HPS) case in Dade County, Florida. Cotton rats (Sigmodon hispidus) were the most frequently capture rodent and displayed the highest seroprevalence to a variety of hantavirus antigens. Hantavirus genome RNA was detected in all the seropositive cotton rats tested, using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay. A virus was isolated from tissues of two seropositive cotton rats by cultivation of lung and spleen homogenates on Vero E6 cells. Nucleotide sequence information obtained by direct RT-PCR and the serologic relationships of this virus with the other hantaviruses indicate that this virus, Black Creek Canal virus, represents a new hantavirus distinct from the previously known serotypes.
authors.affiliation: Special Pathogens Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
authors.forename: P E
authors.initials: PE
authors.lastname: Rollin
authors.forename: T G
authors.initials: TG
authors.lastname: Ksiazek
authors.forename: L H
authors.initials: LH
authors.lastname: Elliott
authors.forename: E V
authors.initials: EV
authors.lastname: Ravkov
authors.forename: M L
authors.initials: ML
authors.lastname: Martin
authors.forename: S
authors.initials: S
authors.lastname: Morzunov
authors.forename: W
authors.initials: W
authors.lastname: Livingstone
authors.forename: M
authors.initials: M
authors.lastname: Monroe
authors.forename: G
authors.initials: G
authors.lastname: Glass
authors.forename: S
authors.initials: S
authors.lastname: Ruo
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D002522:Chlorocebus aethiops; D004197:Disease Reservoirs; D004797:Enzyme-Linked Immunosorbent Assay; D005431:Florida; D006476:Orthohantavirus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D008168:Lung / Q000821:virology; D051379:Mice; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D051381:Rats; D016205:Sigmodontinae / Q000821:virology*; D013154:Spleen / Q000821:virology; D014709:Vero Cells
chemical_list: D000956:Antigens, Viral; D012367:RNA, Viral