title: Selective separation of human peripheral platelets, granulocytes and lymphocytes by surface affinity chromatography.
abstract: Selective separation of human peripheral platelets, granulocytes and lymphocytes was investigated by column liquid chromatography using methoxyethoxymethyl (MEM) bonded-phase columns (25 x 0.9 cm I.D.). Isotonic solutions containing mono- and disaccharides, methyl-alpha-D-pyranosides and a physiological saline at pH 7.4 were used as the mobile phase. Granulocytes and lymphocytes were separated on the MEM-Cellulofine GH-25 column by elution with 0.3 M D-mannose solution. The isolation of platelets and lymphocytes from human leukocyte-rich plasma was performed with a MEM-Sephadex G25 column and elution with 0.27 M sucrose solution. On the same column platelets could also be collected selectively by elution with 0.31 M methyl-alpha-D-mannoside at the high recovery of 100%. The isolated cells were viable for more than 90%.
authors.affiliation: Department of Analytical Chemistry, Tokyo College of Pharmacy, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Shibusawa
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Kinoshita
authors.forename: U
authors.initials: U
authors.lastname: Matsumoto
mesh_terms: D001792:Blood Platelets / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D002852:Chromatography, Ion Exchange; D006098:Granulocytes / Q000166:cytology*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*