Suppression of eosinophil function by RP 73401, a potent and selective inhibitor of cyclic AMP-specific phosphodiesterase: comparison with rolipram.
1. We have investigated the inhibitory potency of RP 73401, a novel, highly selective and potent inhibitor of cyclic AMP-specific phosphodiesterase (PDE IV), against partially-purified PDE isoenzymes from smooth muscle and the particulate PDE IV from guinea-pig eosinophils. The inhibitory effects of RP 73401 on the generation of superoxide (.O2-), major basic protein (MBP) and eosinophil cationic protein (ECP) from guinea-pig eosinophils have also been studied. 2. RP 73401 potently inhibited partially-purified cyclic AMP-specific phosphodiesterase (PDE IV) from pig aortic smooth muscle (IC50 = 1.2 nM); it was similarly potent against the particulate PDE IV from guinea-pig peritoneal eosinophils (IC50 = 0.7 nM). It displayed at least a 19000 fold selectivity for PDE IV compared to its potencies against other PDE isoenzymes. Rolipram was approximately 2600 fold less potent than RP 73401 against pig aortic smooth muscle PDE IV (IC50 = 3162 nM) and about 250 times less potent against eosinophil PDE IV (IC50 = 186 nM). 3. Solubilization of the eosinophil particulate PDE IV increased the potency of rolipram 10 fold but did not markedly affect the potency of RP 73401. A similar (10 fold) increase in the PDE IV inhibitory potency of rolipram, but not RP 73401, was observed when eosinophil membranes were exposed to vanadate/glutathione complex (V/GSH). 4. Reverse transcription polymerase chain reaction (RT-PCR), using primer pairs designed against specific sequences in four distinct rat PDE IV subtype cDNA clones (PDE IVA-D), showed only mRNA for PDE IVD in guinea-pig eosinophils. PDE IVD was also the predominant subtype expressed in pig aortic smooth muscle cells. 5. RP 73401 (Kiapp = 0.4 nM) was 4 fold more potent than (+/-)-rolipram (Kiapp = 1.7 nM) in displacing[3H]-(+/-)-rolipram from guinea-pig brain membranes.6. In intact eosinophils, RP 73401 potentiated isoprenaline-induced cyclic AMP accumulation(EC50 = 79 nM). RP 73401 also inhibited leukotriene B4-induced generation of *02- (IC50 = 25 nM), and the release of major basic protein (ICo = 115 nM) and eosinophil cationic protein (IC50 = 7 nM). Rolipram was 3-14 times less potent than RP 73401.7. Thus RP 73401 is a very potent and selective PDE IV inhibitor which suppresses eosinophil function suggesting that it may be a useful agent for the treatment of inflammatory diseases such as asthma. The greatly different inhibitory potencies of rolipram against PDE IV from smooth muscle and eosinophils(in contrast to the invariable effects of RP 73401) are unlikely to be attributable to diverse PDE IV subtypes but suggest distinct interactions of the two inhibitors with the enzyme.
Souness
J E
JE
Rhône-Poulenc Rorer Central Research, Dagenham Research Centre, Essex.
Maslen
C
C
Webber
S
S
Foster
M
M
Raeburn
D
D
Palfreyman
M N
MN
Ashton
M J
MJ
Karlsson
J A
JA
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors* / Q000145:classification / Q000378:metabolism; D000818:Animals; D001011:Aorta; D001483:Base Sequence; D001549:Benzamides / Q000494:pharmacology*; D001798:Blood Proteins / Q000378:metabolism; D002417:Cattle; D000242:Cyclic AMP / Q000378:metabolism; D017931:DNA Primers; D047091:Eosinophil Granule Proteins; D004804:Eosinophils / Q000187:drug effects*; D005978:Glutathione / Q000378:metabolism; D006168:Guinea Pigs; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D008969:Molecular Sequence Data; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000201:enzymology; D011485:Protein Binding; D011725:Pyridines / Q000494:pharmacology*; D011760:Pyrrolidinones / Q000378:metabolism / Q000494:pharmacology*; D012260:Ribonucleases; D020889:Rolipram; D013481:Superoxides / Q000378:metabolism; D013552:Swine; D014638:Vanadates / Q000378:metabolism
D001549:Benzamides; D001798:Blood Proteins; D017931:DNA Primers; D047091:Eosinophil Granule Proteins; D011725:Pyridines; D011760:Pyrrolidinones; D016210:Methacholine Chloride; D013481:Superoxides; D014638:Vanadates; D000242:Cyclic AMP; D012260:Ribonucleases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D005978:Glutathione; D020889:Rolipram; C087566:piclamilast