Purification of preparative quantities of group B Streptococcus type III oligosaccharides.
Many bacterial capsular polysaccharides are regularly repeating units of oligosaccharides. Bacterial oligosaccharides have been used in neoglycoconjugate vaccines and as reagents in the study of specific antibody binding. Unfortunately, separation methods have not been adequate for the purification of preparative quantities of bacterial oligosaccharides. Here we describe a size-exclusion procedure that resulted in the resolution of group B Streptococcus type III oligosaccharides composed of 4-25 sugars.
Paoletti
L C
LC
Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Johnson
K D
KD
D002240:Carbohydrate Sequence; D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019158:N-Acetylneuraminic Acid; D009844:Oligosaccharides / Q000302:isolation & purification*; D012794:Sialic Acids / Q000737:chemistry; D013292:Streptococcus agalactiae / Q000737:chemistry*
D009844:Oligosaccharides; D012794:Sialic Acids; D019158:N-Acetylneuraminic Acid