The S-locus of Nicotiana alata: genomic organization and sequence analysis of two S-RNase alleles.
Genomic clones encoding the S2- and S6-RNases of Nicotiana alata Link and Otto, which are the allelic stylar products of the self-incompatibility (S) locus, were isolated and sequenced. Analysis of genomic DNA by pulsed-field gel electrophoresis and Southern blotting indicates the presence of only a single S-RNase gene in the N. alata genome. The sequences of the open-reading frames in the genomic and corresponding cDNA clones were identical. The organization of the genes was similar to that of other S-RNase genes from solanaceous plants. No sequence similarity was found between the DNA flanking the S2- and S6-RNase genes, despite extensive similarities between the coding regions. The DNA flanking the S6-RNase gene contained sequences that were moderately abundant in the genome. These repeat sequences are also present in other members of the Nicotianae.
Matton
D P
DP
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia.
Mau
S L
SL
Okamoto
S
S
Clarke
A E
AE
Newbigin
E
E
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010947:Plants, Toxic; D012091:Repetitive Sequences, Nucleic Acid; D012260:Ribonucleases / Q000737:chemistry / Q000235:genetics*; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics*
D018076:DNA, Complementary; D012260:Ribonucleases; C022542:ribonuclease S