title: Selective neutralization of a bacterial enterotoxin by serum immunoglobulin A in response to mucosal disease.
abstract: One-third of convalescent-phase serum samples (6 of 18) from patients with Clostridium difficle-associated diarrhea demonstrated neutralization of the clostridial enterotoxin, toxin A. Although appreciable amounts of toxin A-specific immunoglobulin G (IgG) and IgA were present in these sera, the ability to neutralize the cytotoxic activity of toxin A on OTF9-63 cells in vitro was confined to the IgA fraction and the IgA1 subclass in serum samples from all six patients. In contrast to the patients with C. difficile diarrhea, this activity was present in both the IgA and IgG fractions in sera from two C. difficile-infected patients without diarrhea, one of whom presented with a splenic abscess. Sera and purified IgA which neutralized the cytotoxicity of toxin A on OTF9-63 cell cultures in vitro also neutralized the enterotoxicity of toxin A in rabbit ileal loops in vivo. This activity was not Fc dependent, since IgA retained neutralizing activity after pepsin digestion and F(ab')2 purification. The transition from nonneutralizing toxin A-specific IgA in the acute-phase sera to neutralizing specific IgA in the convalescent-phase sera was accompanied by a shift from a polymeric to a predominantly monomeric form of specific IgA. However, the neutralizing activity in convalescent-phase sera was present as both monomeric and polymeric IgA. Convalescent-phase sera from other patients with C. difficile diarrhea that failed to neutralize toxin A also failed to produce a predominantly monomeric-form specific IgA response. We conclude that serum IgA, not IgG, characteristically neutralizes toxin A in patients with C. difficile diarrhea who develop neutralizing systemic responses. This neutralization of an enteric bacterial toxin is a unique and selective role for serum IgA which provides a novel functional link between the systemic and mucosal immune systems.
authors.affiliation: Department of Medicine, Veterans Affairs Medical Center, Minneapolis, Minnesota 55417, USA.
authors.forename: S
authors.initials: S
authors.lastname: Johnson
authors.forename: W D
authors.initials: WD
authors.lastname: Sypura
authors.forename: D N
authors.initials: DN
authors.lastname: Gerding
authors.forename: S L
authors.initials: SL
authors.lastname: Ewing
authors.forename: E N
authors.initials: EN
authors.lastname: Janoff
mesh_terms: D000208:Acute Disease; D000818:Animals; D000937:Antigen-Antibody Reactions; D001427:Bacterial Toxins / Q000276:immunology*; D016360:Clostridioides difficile / Q000276:immunology*; D003967:Diarrhea / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology*; D006801:Humans; D007082:Ileum / Q000187:drug effects / Q000473:pathology; D007070:Immunoglobulin A / Q000276:immunology*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D008970:Molecular Weight; D011817:Rabbits
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; D007070:Immunoglobulin A; D007140:Immunoglobulin Fab Fragments; C034639:polymeric IgA; C041348:tcdA protein, Clostridium difficile