Stimulation of elastin expression by minoxidil in chick skin fibroblasts.
Minoxidil inhibited the proliferation of embryonic skin fibroblasts during the growth phase but not during the stationary phase. Minoxidil stimulated elastin synthesis two-fold in a dose-dependent manner at a concentration of 1 mM during the stationary phase. The stimulation of elastin synthesis paralleled a comparable increase in elastin mRNA level. These results suggest that the stimulation of elastin expression by minoxidil in skin fibroblasts was controlled at the elastin mRNA level and also suggest that its elastin-stimulating effect is not related to the suppressive effect on cell proliferation. Minoxidil appears to be a potent stimulator for elastin expression in skin fibroblasts.
Tajima
S
S
Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
Hayashi
A
A
Suzuki
T
T
Nishikawa
T
T
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D004549:Elastin / Q000096:biosynthesis* / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism; D008914:Minoxidil / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D012867:Skin / Q000378:metabolism
D012333:RNA, Messenger; D008914:Minoxidil; D004549:Elastin