Evaluation of the polarographic technique for assay of the viability of freeze-dried BCG vaccine: I. The polarographic technique.
In this paper, a new assay based on oxygen uptake assessed by polarography was evaluated with the aim of establishing the viability of freeze-dried BCG vaccine. An oxygen electrode possessing a temperature sensor was designed for this purpose. The polarographic method used had several advantages, particularly its rapidity and use of small amounts of biological material. These advantages are ideal for quality control of BCG vaccine.
Malucelli
M I
MI
Instituto Butantan, Divisão de Desenvolvimento Tecnológico e Produção, São Paulo, Brazil.
Niero
R
R
Lucchiari
P H
PH
Bacila
M
M
D001500:BCG Vaccine; D004566:Electrodes; D005069:Evaluation Studies as Topic; D005612:Freeze Drying; D009163:Mycobacterium bovis / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D010100:Oxygen / Q000032:analysis / Q000493:pharmacokinetics; D011048:Polarography / Q000295:instrumentation / Q000379:methods*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D001500:BCG Vaccine; D010100:Oxygen