title: A novel protein, amyloid precursor-like protein 2, is present in human brain, cerebrospinal fluid and conditioned media.
abstract: A monoclonal antibody, 3B11, was raised to a novel protein, amyloid precursor-like protein 2, which did not recognize amyloid precursor protein. Multiple bands were detected in human brain fractions and cell lysate by Western blotting, indicating the presence of isoforms, 3B11 immunoreactivity was also detected in cerebrospinal fluid and conditioned medium, indicating that the protein is secreted. Immunocytochemistry revealed 3B11 immunoreactivity in sections of human brain.
authors.affiliation: Miriam Marks Department of Neurochemistry, Institute of Neurology, London, U.K.
authors.forename: M T
authors.initials: MT
authors.lastname: Webster
authors.forename: N
authors.initials: N
authors.lastname: Groome
authors.forename: P T
authors.initials: PT
authors.lastname: Francis
authors.forename: B R
authors.initials: BR
authors.lastname: Pearce
authors.forename: F E
authors.initials: FE
authors.lastname: Sherriff
authors.forename: G
authors.initials: G
authors.lastname: Thinakaran
authors.forename: K M
authors.initials: KM
authors.lastname: Felsenstein
authors.forename: W
authors.initials: W
authors.lastname: Wasco
authors.forename: R E
authors.initials: RE
authors.lastname: Tanzi
authors.forename: D M
authors.initials: DM
authors.lastname: Bowen
mesh_terms: D000595:Amino Acid Sequence; D016564:Amyloid beta-Protein Precursor / Q000031:analogs & derivatives* / Q000134:cerebrospinal fluid / Q000276:immunology / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D016466:CHO Cells; D002999:Clone Cells; D006224:Cricetinae; D017077:Culture Media, Conditioned; D017931:DNA Primers; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008969:Molecular Sequence Data; D016716:PC12 Cells; D051381:Rats; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured
chemical_list: C489697:APLP1 protein, human; D016564:Amyloid beta-Protein Precursor; D000911:Antibodies, Monoclonal; C078201:Aplp1 protein, mouse; D017077:Culture Media, Conditioned; D017931:DNA Primers