Bovine T cells preferentially recognize non-viral spacer epitopes in a putative FMDV vaccinal peptide.
In a group of immunized cattle with a variety of MHC class II types, T-cell responses were detected to a synthetic peptide (FMDV15) proposed as a basis for a vaccine against foot-and-mouth disease. This peptide combines the loop region of VP1 with the C-terminal sequence connected by a spacer (PPS). Two major immunodominant regions of FMDV15 for bovine T cells were detected, one within the loop region and the other around the spacer. A substantial proportion of the T-cell response to FMDV15 was directed to the spacer region in 5/12 animals, and in vitro generation of FMDV15-specific T cells preferentially selected for spacer-specific T cells. This pattern of response was associated with a particular MHC class II type which is very common in cattle. Focusing of the T-cell response to non-native virus epitopes may explain why FMDV15 is inefficient at inducing protection.
Glass
E J
EJ
Roslin Institute, Midlothia, UK.
Millar
P
P
D000595:Amino Acid Sequence; D000818:Animals; D002213:Capsid / Q000276:immunology* / Q000494:pharmacology; D036022:Capsid Proteins; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control / Q000821:virology; D005260:Female; D005536:Foot-and-Mouth Disease / Q000517:prevention & control / Q000821:virology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D016056:Immunodominant Epitopes / Q000276:immunology*; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000276:immunology* / Q000494:pharmacology; D010849:Picornaviridae / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology* / Q000494:pharmacology; D014765:Viral Vaccines / Q000276:immunology* / Q000494:pharmacology
D036022:Capsid Proteins; C094837:FMDV 15 peptide; D000949:Histocompatibility Antigens Class II; D016056:Immunodominant Epitopes; D010446:Peptide Fragments; D014764:Viral Proteins; D014765:Viral Vaccines