title: Two genes involved in the phase-variable phi C31 resistance mechanism of Streptomyces coelicolor A3(2).
abstract: The phage growth limitation (Pgl) system of Streptomyces coelicolor confers resistance to phi C31 and its homoimmune phages. The positions of the pgl genes within a 16-kb clone of S. coelicolor DNA were defined by subcloning, insertional inactivation, and deletion mapping. Nucleotide sequencing and functional analysis identified two genes, pglY and pglZ, required for the Pgl+ (phage-resistant) phenotype. pglY and pglZ, which may be translationally coupled, are predicted to encode proteins with M(r)S of 141,000 and 104,000, respectively. Neither protein shows significant similarity to other known proteins, but PglY has a putative ATP/GTP binding motif. The pglY and pglZ genes are cotranscribed from a single promoter which appears to be constitutive and is not induced by phage infection.
authors.affiliation: Department of Genetics, John Innes Centre, Colney, Norwich, United Kingdom.
authors.forename: D J
authors.initials: DJ
authors.lastname: Bedford
authors.forename: C
authors.initials: C
authors.lastname: Laity
authors.forename: M J
authors.initials: MJ
authors.lastname: Buttner
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001435:Bacteriophages / Q000472:pathogenicity*; D001483:Base Sequence; D002352:Carrier Proteins; D003001:Cloning, Molecular; D005798:Genes, Bacterial / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016366:Open Reading Frames / Q000235:genetics; D009876:Operon / Q000235:genetics; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D014176:Protein Biosynthesis; D011995:Recombination, Genetic; D015183:Restriction Mapping; D017384:Sequence Deletion; D013302:Streptomyces / Q000235:genetics* / Q000821:virology*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; C095144:PglY protein, Streptomyces coelicolor; C095145:PglZ protein, Streptomyces coelicolor