title: Lipid composition and fatty acid analysis of Helicobacter pylori.
abstract: Lipids extracted from Helicobacter pylori were separated into lipid classes by thin-layer chromatography. Simple H. pylori lipids consisted of cholesterol esters, triglycerides, free fatty acids, cholesterol, diacylglycerols, and monoacylglycerols. Fatty acids were released from each lipid class by acid methanolysis, and analyzed by gas liquid chromatography and mass spectrometry. Unique methoxy fatty acids, including 11-methoxy heptadecanoic and 11-methoxy nonadecanoic acids, were the major components of the cholesterol esters and triglycerides. The predominance of methoxy fatty acids in the cholesterol esters of H. pylori may contribute to the acid-resistant characteristic of this bacillus.
authors.affiliation: Department of Internal Medicine, Yamaguchi Rosai Hospital, Onoda, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Inamoto
authors.forename: S
authors.initials: S
authors.lastname: Hamanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Hamanaka
authors.forename: T
authors.initials: T
authors.lastname: Nagate
authors.forename: I
authors.initials: I
authors.lastname: Kondo
authors.forename: T
authors.initials: T
authors.lastname: Takemoto
authors.forename: K
authors.initials: K
authors.lastname: Okita
mesh_terms: D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D016480:Helicobacter pylori / Q000737:chemistry* / Q000378:metabolism; D008055:Lipids / Q000032:analysis*; D009163:Mycobacterium bovis / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D005227:Fatty Acids; D008055:Lipids