title: Sensitive detection and structural characterization of trimethyl(p-aminophenyl)-ammonium-derivatized oligosaccharides by electrospray ionization-mass spectrometry and tandem mass spectrometry.
abstract: Mass spectrometric methodologies which use electrospray ionization (ESI) for oligosaccharide structural determination and which are linked to classical procedures, such as reductive amination, have been established. Several derivatives of a model oligosaccharide were prepared and their ionization efficiencies in electrospray ionization (ESI) and fast-atom bombardment (FAB) mass spectrometry were investigated. The trimethyl-(p-aminophenyl)ammonium (TMAPA) derivatives showed extremely high sensitivity in positive-ion ESI-MS and gave Y- and Z-series sequence ions by ESI tandem mass spectromety (MS/MS). This study suggests that TMAPA-derivatized oligosaccharides used in ESI-MS and ESI-MS/MS would be effective for structural characterization of oligosaccharides that are available only in limited quantities.
authors.affiliation: Environmental Health Science Laboratory, Sumitomo Chemical Co. Ltd, Osaka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Okamoto
authors.forename: K
authors.initials: K
authors.lastname: Takahashi
authors.forename: T
authors.initials: T
authors.lastname: Doi
mesh_terms: D000631:Aminopyridines / Q000737:chemistry; D001566:Benzocaine; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005456:Fluorescent Dyes; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000737:chemistry*
chemical_list: D000631:Aminopyridines; D005456:Fluorescent Dyes; C095297:N-(4-(trimethylammonio)phenyl)maltopentaosamine; D009844:Oligosaccharides; C095296:peracetyl maltopentaose; D001566:Benzocaine; C032439:alpha-aminopyridine