title: Circuit simulation of genetic networks.
abstract: Genetic networks with tens to hundreds of genes are difficult to analyze with currently available techniques. Because of the many parallels in the function of these biochemically based genetic circuits and electrical circuits, a hybrid modeling approach is proposed that integrates conventional biochemical kinetic modeling within the framework of a circuit simulation. The circuit diagram of the bacteriophage lambda lysislysogeny decision circuit represents connectivity in signal paths of the biochemical components. A key feature of the lambda genetic circuit is that operons function as active integrated logic components and introduce signal time delays essential for the in vivo behavior of phage lambda.
authors.affiliation: Department of Developmental Biology, Beckman Center, Stanford University School of Medicine 94305, USA.
authors.forename: H H
authors.initials: HH
authors.lastname: McAdams
authors.forename: L
authors.initials: L
authors.lastname: Shapiro
mesh_terms: D001433:Bacteriolysis; D010582:Bacteriophage lambda / Q000235:genetics* / Q000502:physiology; D003198:Computer Simulation; D004254:DNA Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins; D005246:Feedback; D015967:Gene Expression Regulation, Viral; D019426:Integrases; D008242:Lysogeny / Q000235:genetics*; D008433:Mathematics; D008957:Models, Genetic; D009876:Operon; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D012984:Software; D013728:Terminator Regions, Genetic; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D054334:Viral Regulatory and Accessory Proteins; D014775:Virus Activation; D016662:Virus Integration
chemical_list: D004268:DNA-Binding Proteins; C066119:Q protein, Bacteriophage lambda; D012333:RNA, Messenger; D012367:RNA, Viral; D012097:Repressor Proteins; D014157:Transcription Factors; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C039000:cII protein, bacteriophage lambda; C054615:cIII protein, Bacteriophage lambda; C016649:phage repressor proteins; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C063911:excisionase