Proteoglycan metabolism of equine articular cartilage and its modulation by insulin-like growth factors.
The effect of human recombinant insulin-like growth factor 1 (rhIGF-1) on proteoglycan (PG) metabolism of full thickness equine articular cartilage explants was investigated. PG synthesis was stimulated at all ages, but higher concentrations of rhIGF-1 were required for maximal stimulation of adult cartilage. There were no changes in the hydrodynamic size, electrophoretic heterogeneity or composition of proteoglycans isolated from rhIGF-1-stimulated cartilage. rhIGF-1 reduced the rate of turnover of both newly synthesized and endogenous proteoglycans in all ages of cartilage investigated. The structure of proteoglycan fragments retained within the matrix and those released into the culture medium was unaffected by IGF-1 stimulation, suggesting that this peptide is a key regulator of the proteoglycan composition of equine articular cartilage extracellular matrix.
Platt
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D
Royal Veterinary College, University of London, Hatfield, Hertfordshire, UK.
Bayliss
M T
MT
D000375:Aging / Q000378:metabolism; D000818:Animals; D002358:Cartilage, Articular / Q000187:drug effects* / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000032:analysis; D002850:Chromatography, Gel / Q000662:veterinary; D002852:Chromatography, Ion Exchange / Q000662:veterinary; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D006207:Half-Life; D006736:Horses / Q000378:metabolism*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000494:pharmacology*; D007553:Isotope Labeling / Q000662:veterinary; D011509:Proteoglycans / Q000096:biosynthesis / Q000378:metabolism*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology
D003470:Culture Media; D011509:Proteoglycans; D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I; D002809:Chondroitin Sulfates