Tissue-regulated differentiation and maturation of a v-abl-immortalized mast cell-committed progenitor.
An immature v-abl-transformed mast cell line (V3-MC) was derived from a mouse that developed systemic mastocytosis after transplantation of v-abl-infected bone marrow cells. V3-MCs injected intravenously into adult BALB/c mice infiltrated the liver, spleen, and intestine by day 6 and underwent progressive differentiation and maturation, eventually resembling indigenous mast cells. In terms of their protease content, the V3-MCs that localized in the liver and spleen differed from those in the intestine, and both differed from the cultured V3-MCs. The acquired expression of certain proteases and the loss of expression of other proteases in these tissue V3-MCs defines particular phenotypes and indicates that the differentiation and maturation of mast cell-committed progenitor cells are primarily regulated by factors in the different tissue microenvironments.
Gurish
M F
MF
Department of Rheumatology and Immunology, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
Pear
W S
WS
Stevens
R L
RL
Scott
M L
ML
Sokol
K
K
Ghildyal
N
N
Webster
M J
MJ
Hu
X
X
Austen
K F
KF
Baltimore
D
D
D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D017931:DNA Primers / Q000737:chemistry; D015870:Gene Expression; D016313:Genes, abl; D007150:Immunohistochemistry; D008407:Mast Cells / Q000166:cytology*; D008415:Mastocytosis / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D014018:Tissue Distribution
D017931:DNA Primers; D009856:Oncogene Proteins, Viral; D012333:RNA, Messenger; D012697:Serine Endopeptidases