title: Kaposi's sarcoma-associated herpesvirus-like DNA sequences in multicentric Castleman's disease.
abstract: Multicentric Castleman's disease (MCD) is an atypical lymphoproliferative disorder defined using clinical and pathologic criteria. A characteristic of the MCD is a close association with Kaposi's sarcoma (KS), which occurs during the clinical course of most human immunodeficiency virus (HIV)-associated MCD cases and also, but less frequently, in HIV-negative patients. Recently, sequences of a putative new Herpesvirus (KSHV) have been isolated and further detected in almost all the acquired immunodeficiency syndrome (AIDS) KS and in most of the non-AIDS KS samples. In this study, we searched for these Herpesvirus-like sequences in MCD samples of 31 patients. KSHV sequences were detected in 14 of 14 cases of HIV-associated MCD, including 5 cases without detectable KS. Moreover, KSHV was detected in 7 of 17 MCD cases in HIV-negative patients, including 1 case associated with a cutaneous KS. In 34 non-MCD reactive lymph nodes (follicular and/or interfollicular hyperplasia) in HIV-negative patients, KSHV was detected in only 1 case. In 1 HIV-negative case of MCD, KSHV was found in both the lymph node and peripheral blood samples. These data suggest that KSHV could play a role in the pathogenesis of MCD, especially in HIV-infected patients.
authors.affiliation: Laboratoire d'Hématologie Moléculaire, Hôpital Saint-Louis, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Soulier
authors.forename: L
authors.initials: L
authors.lastname: Grollet
authors.forename: E
authors.initials: E
authors.lastname: Oksenhendler
authors.forename: P
authors.initials: P
authors.lastname: Cacoub
authors.forename: D
authors.initials: D
authors.lastname: Cazals-Hatem
authors.forename: P
authors.initials: P
authors.lastname: Babinet
authors.forename: M F
authors.initials: MF
authors.lastname: d'Agay
authors.forename: J P
authors.initials: JP
authors.lastname: Clauvel
authors.forename: M
authors.initials: M
authors.lastname: Raphael
authors.forename: L
authors.initials: L
authors.lastname: Degos
mesh_terms: D000328:Adult; D000368:Aged; D001483:Base Sequence; D005871:Castleman Disease / Q000382:microbiology*; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D015658:HIV Infections / Q000150:complications; D006564:Herpesviridae / Q000235:genetics*; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012514:Sarcoma, Kaposi / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral