[2,4,6-trinitrotoluene and 2,4-diamino-6-nitrotoluene: the absence of recA-dependent mutagenesis?].
The genotoxicity of 2,4,6-trinitrotoluene (2,4,6-TNT) and its amino derivative, 2,4-diamino-6-nitrotoluene (2,4-DA-6-NT), was studied using the Escherichia coli tester strain PQ37 in the SOS chromotest. The compound 2,4,6-TNT, without metabolic activation, virtually failed to induce an SOS effect in cells of the tester bacteria. Consequently, mutagenic activity of 2,4,6-TNT, which was shown earlier in the Ames test, does not depend on SOS mutagenesis. It was demonstrated that metabolic activation with the microsomal S9 human placenta fraction results in a threefold increase in the induction factor of the SOS effect caused by 2,4,6-TNT. The absence of the SOS-inducing activity of 2,4-DA-6-NT, regardless of the presence of a microsomal activating mixture, is shown. Thus, 2,4-DA-6-NT does not belong to metabolites of 2,4,6-TNT, responsible for the genotoxicity of this compound.
Karamova
N S
NS
Mynina
I I
II
Garaeva
G G
GG
Ivanchenko
O B
OB
Il'inskaia
O N
ON
D001711:Biotransformation; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D006801:Humans; D008861:Microsomes / Q000378:metabolism; D016296:Mutagenesis; D009153:Mutagens / Q000493:pharmacokinetics / Q000633:toxicity*; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D011938:Rec A Recombinases / Q000235:genetics*; D013014:SOS Response, Genetics; D014052:Toluidines / Q000493:pharmacokinetics / Q000633:toxicity*; D014303:Trinitrotoluene / Q000493:pharmacokinetics / Q000633:toxicity*
D009153:Mutagens; D014052:Toluidines; D014303:Trinitrotoluene; C046358:2,4-diamino-6-nitrotoluene; D011938:Rec A Recombinases
1,4,6-Trinitrotoluol i 2,4-diamino-6-nitrotoluol: otsutstvie recA-zavisimogo mutageneza?