Erythrocyte-mediated toxification of 1,8-dinitropyrene. II. Covalent bind to erythrocyte-proteins.
In the present study we have shown that erythrocyte-catalyzed 1,8-dinitropyrene reduction occurs via formation of reactive intermediate species, which bind covalently to haemoglobin and other erythrocyte proteins. In fact after mild-acid hydrolysis of lysate proteins exposed to DNP, the reduced metabolites, 1-amino-8-nitropyrene and 1,8-diaminopyrene, were released, thus indicating the formation of sulphinamide adducts to proteins. These results suggest that haemoglobin adduct biomonitoring would be a useful method of controlling exposure to nitroarene in persons at risk.
Belisario
M A
MA
Dipartimento di Biochimica e Biotechnologie Mediche, Università di Napoli Federico II.
Garofalo
A
A
Maturo
M
M
Avagnale
G
G
Sannolo
N
N
Malorni
A
A
D000588:Amines / Q000032:analysis; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D011721:Pyrenes / Q000378:metabolism*
D000588:Amines; D011506:Proteins; D011721:Pyrenes; C033310:1,8-dinitropyrene