title: Cloning and characterisation of a fish aldolase B gene.
abstract: A full length cDNA clone representing an aldolase mRNA was isolated from a sea bream (Sparus aurata) liver cDNA library. Sequencing of this clone revealed it to encode a 364 amino acid protein with 74% amino acid identity to human aldolase B and slightly lower similarity to human aldolase A and C. In view of the sequence data and of Northern blot analysis showing strong expression of a 1.6 kb transcript in liver it was concluded that the cloned gene represents aldolase B. This clone represents the first aldolase gene to be sequenced from any fish species thus providing new data on the evolution of the vertebrate aldolase gene family.
authors.affiliation: School of Molecular and Medical Biosciences, University of Wales, Cardiff, UK.
authors.forename: L
authors.initials: L
authors.lastname: Llewellyn
authors.forename: V P
authors.initials: VP
authors.lastname: Ramsurn
authors.forename: G E
authors.initials: GE
authors.lastname: Sweeney
authors.forename: T
authors.initials: T
authors.lastname: Wigham
authors.forename: C R
authors.initials: CR
authors.lastname: Santos
authors.forename: D M
authors.initials: DM
authors.lastname: Power
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005399:Fishes / Q000235:genetics*; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics*; D008099:Liver / Q000201:enzymology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000302:isolation & purification; D016415:Sequence Alignment
chemical_list: D012333:RNA, Messenger; D005634:Fructose-Bisphosphate Aldolase