title: Construction and characterization of an isogenic urease-negative mutant of Helicobacter mustelae.
abstract: Helicobacter mustelae infects the ferret stomach and provides an opportunity to study pathogenic determinants of a Helicobacter species in its natural host. We constructed an isogenic urease-negative mutant of H. mustelae which produced no detectable urease and showed a reduced acid tolerance. This mutant provides an opportunity to further evaluate the role of urease in the pathogenesis of Helicobacter infection.
authors.affiliation: Division of Infectious Diseases, University of California, Davis, School of Medicine, USA.
authors.forename: J V
authors.initials: JV
authors.lastname: Solnick
authors.forename: C
authors.initials: C
authors.lastname: Josenhans
authors.forename: S
authors.initials: S
authors.lastname: Suerbaum
authors.forename: L S
authors.initials: LS
authors.lastname: Tompkins
authors.forename: A
authors.initials: A
authors.lastname: Labigne
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004251:DNA Transposable Elements; D016998:Helicobacter / Q000201:enzymology* / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D009154:Mutation; D014510:Urease / Q000235:genetics* / Q000502:physiology
chemical_list: D004251:DNA Transposable Elements; D014510:Urease