Proliferation of MIELIKI a novel t(7;9) early pre-B acute lymphoblastic leukemia cell line is inhibited concomitantly by IL-4 and IL-7.
The present study describes a novel cell line, MIELIKI, established from bone marrow of a pediatric patient with B lineage acute lymphoblastic leukemia (ALL) at diagnosis. The MIELIKI cell line displays an early pre-B cell phenotype (CD10+, CD19+, CD20+, CD34-, Cmu-, sIg-) with rearrangements on both Ig heavy chain and k light chain alleles, and carries an unfrequent t(7;9) chromosomal translocation identical to the freshly isolated leukemic blasts. The proliferation of MIELIKI cells was abrogated by IL-4 and by IL-7, as measured by DNA replication and viable cell recovery. The effects of IL-4 and IL-7 were mediated, respectively, through the CDw124 and CDw127 IL-4 and IL-7 receptor components. Growth inhibition by IL-4 was not mediated by soluble factors released by MIELIKI cells in response to IL-4, suggesting the existence of an intrinsic negative signaling pathway. Finally, neither IL-4 nor IL-7 were found to induce maturation of MIELIKI into cells expressing cytoplasmic or surface membrane mu chain. The present cell line should constitute a useful model of t(7;9) early pre-B ALL and allow investigation of the relationship between IL-4 and IL-7 negative signaling in leukemic B cell ontogeny.
Renard
N
N
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Duvert
V
V
Matthews
D J
DJ
Pagés
M P
MP
Magaud
J P
JP
Manel
A M
AM
Pandrau-Garcia
D
D
Philippe
N
N
Banchereau
J
J
Saeland
S
S
D015703:Antigens, CD / Q000378:metabolism; D002455:Cell Division; D002897:Chromosomes, Human, Pair 7; D002899:Chromosomes, Human, Pair 9; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D015328:Gene Rearrangement, B-Lymphocyte, Light Chain; D006801:Humans; D007223:Infant; D015847:Interleukin-4 / Q000494:pharmacology*; D015851:Interleukin-7 / Q000494:pharmacology*; D007621:Karyotyping; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018123:Receptors, Interleukin / Q000378:metabolism; D019948:Receptors, Interleukin-4; D020395:Receptors, Interleukin-7; D015398:Signal Transduction; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D015703:Antigens, CD; D015851:Interleukin-7; D018123:Receptors, Interleukin; D019948:Receptors, Interleukin-4; D020395:Receptors, Interleukin-7; D015847:Interleukin-4