title: Quantitative biological electron probe microanalysis with a wavelength dispersive spectrometer.
abstract: This paper describes the details of quantitative electron probe microanalysis (EPMA) performed with a wavelength dispersive spectrometer (WDS). EPMA was carried out on the giant neuron of a fresh frozen ganglion from the snail Lymnaea stagnalis. The freeze-dried cryosections were compared with sections of freeze-dried, embedded tissue. It was found, that in the ganglion there are two kinds of neurons with a different chlorine concentration of 11 mmole/liter and 32 mmole/liter. Isolated neurons in culture were shown to differ in elemental composition from those in the ganglion tissue.
authors.affiliation: Institute of Theoretical and Experimental Biophysics of the Russian Academy, Moscow Province.
authors.forename: A
authors.initials: A
authors.lastname: Pogorelov
authors.forename: V
authors.initials: V
authors.lastname: Pogorelova
authors.forename: N V
authors.initials: NV
authors.lastname: Repin
authors.forename: I
authors.initials: I
authors.lastname: Mizin
mesh_terms: D000818:Animals; D004577:Electron Probe Microanalysis / Q000295:instrumentation / Q000379:methods*; D005612:Freeze Drying; D005614:Freeze Fracturing; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000648:ultrastructure; D008195:Lymnaea; D008433:Mathematics; D017348:Microscopy, Electron, Scanning Transmission / Q000379:methods; D009474:Neurons / Q000648:ultrastructure*; D012680:Sensitivity and Specificity