Expression of intercellular adhesion molecule-1 is regulated by the actin network in epidermoid carcinoma cells.
Expression of the cell surface protein intercellular adhesion molecule-1 (ICAM-1) is a prerequisite for the interaction of a large variety of cells with leukocytes. Constitutive expression of ICAM-1 in human epidermoid carcinoma cells is low, but is inducible through inflammatory cytokines including interferon gamma (INF gamma). Disruption of the actin cytoskeleton with dehydrocytochalasin B (CB) increased constitutive and potentiated INF gamma-induced ICAM-1 cell surface expression, but did not alter formation of soluble ICAM-1. Actinomycin D inhibited CB-induced ICAM-1 surface expression and CB increased the steady-state levels of ICAM-1 transcripts. However, CB did not alter the glyceralaldehyde-3-phosphate dehydrogenase mRNA levels and the ICAM-1 mRNA half-life. These studies indicate that in human epidermoid carcinoma cells the actin cytoskeleton regulates ICAM-1 transcription and cell surface expression.
Norgauer
J
J
Department of Dermatology, University of Freiburg, Germany.
Bartels
F
F
Göhring
U
U
Schöpf
E
E
Gebicke-Haerter
P
P
Kownatzki
E
E
Krutmann
J
J
D000199:Actins / Q000502:physiology*; D002294:Carcinoma, Squamous Cell; D002460:Cell Line; D003571:Cytochalasin B / Q000031:analogs & derivatives / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000502:physiology*; D003609:Dactinomycin / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D007371:Interferon-gamma / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D000199:Actins; D012333:RNA, Messenger; D011994:Recombinant Proteins; C094884:dehydrocytochalasin B; D018799:Intercellular Adhesion Molecule-1; D003609:Dactinomycin; D003571:Cytochalasin B; D007371:Interferon-gamma; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases