title: The Newcastle disease virus V protein binds zinc.
abstract: The V protein of Newcastle disease virus (NDV) is produced by the insertion of a single nontemplated G residue at a specific point during transcription of the phosphoprotein (P) gene, accessing a new reading frame upon translation. The V protein, in common with its counterpart in other paramyxoviruses contains a highly cysteine rich motif near the carboxyl terminus, suggestive of a zinc-binding domain. By constructing E. coli overexpression plasmids for the NDV P and V proteins, and monitoring the binding of 65ZnCl2 to proteins electroblotted onto nitrocellulose membranes, we have demonstrated that the V protein strongly binds zinc.
authors.affiliation: Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, U.K.
authors.forename: M
authors.initials: M
authors.lastname: Steward
authors.forename: A C
authors.initials: AC
authors.lastname: Samson
authors.forename: W
authors.initials: W
authors.lastname: Errington
authors.forename: P T
authors.initials: PT
authors.lastname: Emmerson
mesh_terms: D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
chemical_list: C070065:36 kDa V protein, Newcastle disease virus; D009838:Oligodeoxyribonucleotides; C055691:P phosphoprotein, Newcastle disease virus; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D015032:Zinc