Molecular analysis of Japanese isolates of Verticillium dahliae and V. albo-atrum.
Sixteen isolates of different pathogenicity groups of the plant pathogen Verticillium dahliae and four isolates of V. albo-atrum from Japan were analysed by means of an RAPD (random amplified polymorphic DNA) method using a PCR (polymerase chain reaction). Verticillium dahliae and V. albo-atrum could be distinguished by RAPD analysis. Four pathogenicity groups of V. dahliae could also be classified to a certain extent by this method. Similarities and differences in banding patterns obtained by RAPD may be a useful molecular tool in phylogenetic studies of the pathogenicity groups.
Koike
M
M
Department of Agro-environmental Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Watanabe
M
M
Nagao
H
H
Ohshima
S
S
D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D007564:Japan; D000455:Medicago sativa / Q000382:microbiology; D003904:Mitosporic Fungi / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D014675:Vegetables / Q000382:microbiology
D004271:DNA, Fungal