title: A histochemical study of carbonic anhydrase in the plasma membranes of human oral epithelial cells.
abstract: Carbonic anhydrase (EC 4.2.1.1) was detected histochemically from the following regions in patients of various ages (14-84 yr): buccal mucosa, buccal flap, hard palate and tongue. The enzyme was principally located in the cell membranes but was also present in nuclei. There was a gradation in activity from basal (strong) to superficial cells (weak/negative). The carbonic anhydrase inhibitors ethoxyzolamide and acetazolamide abolished activity at 0.001 mM, but were ineffective, even at 1.2 mM, against a reaction associated with the granules of the stratum granulosum. No activity was detected in the absence of bicarbonate from the substrate.
authors.affiliation: Department of Anatomy and Physiology, University, Dundee, Scotland.
authors.forename: K N
authors.initials: KN
authors.lastname: Christie
authors.forename: C
authors.initials: C
authors.lastname: Thomson
authors.forename: G R
authors.initials: GR
authors.lastname: Ogden
authors.forename: D
authors.initials: D
authors.lastname: Hopwood
mesh_terms: D000086:Acetazolamide / Q000494:pharmacology; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002257:Carbonic Anhydrase Inhibitors / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology*; D002467:Cell Nucleus / Q000201:enzymology; D003594:Cytoplasmic Granules / Q000201:enzymology; D004848:Epithelium / Q000201:enzymology; D005016:Ethoxzolamide / Q000494:pharmacology; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000201:enzymology*; D010159:Palate / Q000201:enzymology; D014059:Tongue / Q000201:enzymology
chemical_list: D002257:Carbonic Anhydrase Inhibitors; D002256:Carbonic Anhydrases; D000086:Acetazolamide; D005016:Ethoxzolamide