A single expression system for the display, purification and conjugation of single-chain antibodies.
To facilitate the purification and conjugation of single-chain antibodies (scFv) selected from a phage display library, we have incorporated His6, an amber stop codon and a C-terminal Cys into a surface expression vector. The vector also contains a lacIq gene for improving the efficiency of regulation and a sequence coding for a marker peptide.
Hayashi
N
N
Recombinant Antibody Group, German Cancer Research Center, Heidelberg.
Kipriyanov
S
S
Fuchs
P
P
Welschof
M
M
Dörsam
H
H
Little
M
M
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods; D003090:Coliphages; D017931:DNA Primers; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D005803:Genes, Immunoglobulin; D051379:Mice; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D015183:Restriction Mapping
D000906:Antibodies; D000911:Antibodies, Monoclonal; D017931:DNA Primers; D011993:Recombinant Fusion Proteins