Receptor binding site-deleted foot-and-mouth disease (FMD) virus protects cattle from FMD.
Binding of foot-and-mouth disease virus (FMDV) to cells requires an arginine-glycine-aspartic acid (RGD) sequence in the capsid protein VP1. We have genetically engineered an FMDV in which these three amino acids have been deleted, producing a virus particle which is unable to bind to cells. Cattle vaccinated with these receptor binding site-deleted virions were protected from disease when challenged with a virulent virus, demonstrating that these RGD-deleted viruses could serve as the basis for foot-and-mouth disease vaccines safer than those currently in use. This strategy may prove useful in the development of vaccines for other viral diseases.
McKenna
T S
TS
Plum Island Animal Disease Center, U.S. Department of Agriculture, Greenport, New York 11944, USA.
Lubroth
J
J
Rieder
E
E
Baxt
B
B
Mason
P W
PW
D000595:Amino Acid Sequence; D000818:Animals; D005537:Aphthovirus / Q000235:genetics / Q000276:immunology*; D001665:Binding Sites; D002417:Cattle; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control; D008969:Molecular Sequence Data; D009842:Oligopeptides; D011991:Receptors, Virus / Q000502:physiology*; D017384:Sequence Deletion; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D014771:Virion / Q000235:genetics / Q000276:immunology
D009842:Oligopeptides; D011991:Receptors, Virus; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; C047981:arginyl-glycyl-aspartic acid