Osteopontin expression is increased in the heritable cardiomyopathy of Syrian hamsters.
BACKGROUND
Osteopontin, a noncollagenous matrix protein, is transiently expressed in the heart after experimental cardiac injury, but its expression in states of continuing cardiac remodeling is unknown. We evaluated osteopontin expression in the heritable cardiomyopathy of the Syrian hamster.


RESULTS
Hamster hearts were obtained for RNA isolation and analysis and in situ hybridization from two groups: normal control animals (n = 4) and untreated cardiomyopathic hamsters (n = 5). Osteopontin mRNA was 12-fold greater in cardiomyopathic hearts compared with normal controls (1.76 +/- 0.31 versus 0.14 +/- 0.04 arbitrary units normalized to GAPDH, mean +/- SEM, P < .05). In situ hybridization was used to define the origin of osteopontin in the heart. Osteopontin mRNA above background levels was not detected in sections from noncardiomyopathic hamster hearts but was readily detected in sections from cardiomyopathic hamsters, in which it originated in cells morphologically consistent with tissue macrophages.


CONCLUSIONS
In the hamster, osteopontin is expressed in heritably cardiomyopathic hearts under conditions of chronic injury and repair, and the source of ostopontin message appears to be issue macrophage-like cells in foci of inflammation. This model could be used to evaluate the biological role of osteopontin in myocardial inflammation and remodeling.
Williams
E B
EB
Department of Internal Medicine, Washington University School of Medicine, St Louis, MO, USA.
Halpert
I
I
Wickline
S
S
Davison
G
G
Parks
W C
WC
Rottman
J N
JN
D000818:Animals; D009202:Cardiomyopathies / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D006224:Cricetinae; D017403:In Situ Hybridization; D008297:Male; D008647:Mesocricetus; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D053495:Osteopontin; D012333:RNA, Messenger / Q000378:metabolism; D012795:Sialoglycoproteins / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D012795:Sialoglycoproteins; D053495:Osteopontin