Determination of a novel xanthine derivative, MKS 213-492, in plasma by high-performance liquid chromatography with electrochemical detection.
A reversed-phase liquid chromatographic method with coulometric detection has been developed for the determination of free MKS 213-492 (a novel xanthine derivative) in plasma. This method is based on a simple and rapid plasma extraction procedure using precolumn-switching. The oxidation of this pharmacologically active xanthine derivative was optimized with respect to applied potential, pH of mobile and rinsing phase and rinsing time. The detection limit for MKS 213-492 was found to be 54 pg/injection.
Berliz
G
G
EBEWE Pharmaceuticals, Unterach/Attersee, Austria.
Wintersteiger
R
R
Pacha
W
W
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D010084:Oxidation-Reduction; D010726:Phosphodiesterase Inhibitors / Q000097:blood*; D015203:Reproducibility of Results; D014970:Xanthines / Q000097:blood*
C095074:MKS 213492; D010726:Phosphodiesterase Inhibitors; D014970:Xanthines