SgfI, a new type-II restriction endonuclease that recognizes the octanucleotide sequence 5'-GCGAT/CGC-3'.
A new restriction endonuclease (ENase), SgfI, has been isolated from the bacterium Streptomyces sp. SgfI recognizes the 8-bp palindrome 5'-GCGATCGC-3' and cleaves double-stranded DNA after the T in this sequence, producing a two-base 3' overhang compatible with PvuI termini. SgfI is a rare-cutting ENase and should be useful for megabase mapping experiments.
Kappelman
J R
JR
Promega Corp., Madison, WI 53711-5399, USA.
Brady
M
M
Knoche
K
K
Murray
E
E
Schoenfeld
T
T
Williams
R
R
Vesselinova
N
N
D001483:Base Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008745:Methylation; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D013302:Streptomyces / Q000201:enzymology*; D013379:Substrate Specificity
D009838:Oligodeoxyribonucleotides; D004247:DNA; C061224:endodeoxyribonuclease PvuI; C094985:endodeoxyribonuclease SgfI; D015252:Deoxyribonucleases, Type II Site-Specific