Role of C-terminal direct repeating units of the Streptococcus mutans glucosyltransferase-S in glucan binding.
The C-terminal glucan-binding domain of the glucosyltransferase-S of Streptococcus mutans GS-5 contains five 65-amino-acid direct repeating units. A series of deletion derivatives of both the glucosyltransferase-S and its glucan-binding domain were constructed and analyzed. The results demonstrated that the four C-terminal direct repeating units constituted part of the minimum domain required for glucan binding.
Lis
M
M
Department of Oral Biology, State University of New York, Buffalo 14214, USA.
Shiroza
T
T
Kuramitsu
H K
HK
D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001665:Binding Sites; D003773:Dental Plaque / Q000382:microbiology; D005936:Glucans / Q000378:metabolism*; D005964:Glucosyltransferases / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012091:Repetitive Sequences, Nucleic Acid; D017384:Sequence Deletion; D013295:Streptococcus mutans / Q000201:enzymology* / Q000502:physiology
D001426:Bacterial Proteins; D005936:Glucans; D005964:Glucosyltransferases