Penicillium purpurogenum produces several xylanases: purification and properties of two of the enzymes.
The fungus Penicillium purpurogenum produces several extracellular xylanases. The two major forms (xylanases A and B) have been purified and characterized. After ammonium sulfate precipitation and chromatography in Bio-Gel P 100, xylanase A was further purified by means of DEAE-cellulose, hydroxylapatite and CM-Sephadex, and xylanase B by DEAE-cellulose and CM-Sephadex. Both xylanases showed apparent homogeneity in SDS-polyacrylamide gel electrophoresis. Xylanase A (33 kDa) has an isoelectric point of 8.6, while xylanase B (23 kDa) is isoelectric at pH 5.9. Antisera against both enzymes do not cross-react. The amino terminal sequences of xylanases A and B show no homology. The results obtained suggest that the enzymes are produced by separate genes and they may perform different functions in xylan degradation.
Belancic
A
A
Laboratorio de Bioquímica, Pontificia Universidad Católica de Chile, Santiago.
Scarpa
J
J
Peirano
A
A
Díaz
R
R
Steiner
J
J
Eyzaguirre
J
J
D000595:Amino Acid Sequence; D015153:Blotting, Western; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010407:Penicillium / Q000201:enzymology* / Q000235:genetics; D011134:Polysaccharides / Q000378:metabolism; D016415:Sequence Alignment; D013696:Temperature; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*
D005656:Fungal Proteins; D011134:Polysaccharides; D014990:Xylans; C007916:hemicellulose; D014995:Xylosidases