title: The crystal structure of apo-pseudoazurin from Alcaligenes faecalis S-6.
abstract: The 3D structure of the apo-pseudoazurin (copper free pseudoazurin) from Alcaligenes faecalis strain S-6 is determined and refined at pH 6.7 using X-ray diffraction data to 1.85 A resolution. The final crystallographic R-factor is 0.164. Comparing the structures of apo-pseudoazurin and the native (Cu2+) protein, we observed limited differences ranging between 0.1-0.4 A at the vicinity of the copper site, at the loops connecting the secondary structural elements, at certain beta-strands and at the amino and carboxy termini of the protein.
authors.affiliation: Institute of Molecular Biology and Biotechnology, Heraklion, Greece.
authors.forename: K
authors.initials: K
authors.lastname: Petratos
authors.forename: M
authors.initials: M
authors.lastname: Papadovasilaki
authors.forename: Z
authors.initials: Z
authors.lastname: Dauter
mesh_terms: D000421:Alcaligenes; D001400:Azurin / Q000031:analogs & derivatives* / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D018360:Crystallography, X-Ray; D011487:Protein Conformation
chemical_list: C047163:pseudoazurin; D001400:Azurin; D003300:Copper