Retinoid metabolism in human leucocytes.
Leucocytes from subjects from 0 to 80 years old were separated into mononuclear and granulocyte fractions and the retinoids and retinoid-binding fractions were examined. Both leucocyte fractions were found to contain retinol, retinoic acid and an additional retinoid; retinoic acid comprised 40% of retinoids in some samples. The protein fractions containing retinoids included a 200 kDa protein and several 14-18 kDa proteins. Plasma concentrations of retinol changed little with increasing age. In contrast, leucocyte concentrations of retinoids and retinoid-binding proteins increased quadratically with age. However, in granulocytes from young children retinoids were almost undetectable.
Sklan
D
D
Faculty of Agriculture, Hebrew University, Rehovot, Israel.
Trifon
S
S
Kedar
O
O
Vaisman
N
N
Berner
Y
Y
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D006098:Granulocytes / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007962:Leukocytes / Q000378:metabolism*; D008875:Middle Aged; D012176:Retinoids / Q000378:metabolism*; D012177:Retinol-Binding Proteins / Q000378:metabolism; D054839:Retinol-Binding Proteins, Plasma; D014212:Tretinoin / Q000378:metabolism; D014801:Vitamin A / Q000378:metabolism
D012176:Retinoids; D012177:Retinol-Binding Proteins; D054839:Retinol-Binding Proteins, Plasma; D014801:Vitamin A; D014212:Tretinoin