The cellulase complex of Neurospora crassa: cbh-1 cloning, sequencing and homologies.
We describe the isolation, cloning and sequencing of the cellobiohydrolase 1 (EC 3.2.1.91)-encoding gene (cbh-1) of Neurospora crassa. The nucleotide and amino-acid sequences have high homology with the cbh-1 of Trichoderma reesei, Humicola grisea and Phanerochaete chrysosporium, with clear signal, catalytic, hinge and substrate-binding domains in that order.
Taleb
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Department of Genetics, University of Leeds, UK.
Radford
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A
D000595:Amino Acid Sequence; D001483:Base Sequence; D043366:Cellulose 1,4-beta-Cellobiosidase; D003001:Cloning, Molecular; D017931:DNA Primers; D005800:Genes, Fungal; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D006026:Glycoside Hydrolases; D043366:Cellulose 1,4-beta-Cellobiosidase