title: Correlation of alpha-smooth muscle actin expression and contraction in Dupuytren's disease fibroblasts.
abstract: We studied 11 nodules from patients with Dupuytren's contracture to determine whether alpha-smooth muscle actin expression in Dupuytren's fibroblasts is related to the generation of contractile force. Tissue was placed into explant culture and fibroblast strains were obtained. The mean percent of cultured Dupuytren's fibroblasts expressing alpha-smooth muscle actin, as determined by immunofluorescence, was 14 +/- 8 and ranged from 1% to 26%. The ability of Dupuytren's fibroblasts to generate contractile force was determined by using a previously described collagen lattice contraction assay. We observed a significant positive correlation between the expression of alpha-smooth muscle actin and the generation of contractile force in cell strains of Dupuytren's fibroblasts. In addition, six fibroblast strains from palmar fascia of individuals undergoing carpal tunnel release were examined. In six strains of palmar fibroblasts the mean percent of cells expressing alpha-smooth muscle actin was 5 +/- 3 and ranged from 1% to 9%. Six Dupuytren's fibroblast strains, in which more than 15% of the cells expressed alpha-smooth muscle actin, were significantly more contractile than the palmar fibroblasts. These results suggest that Dupuytren's fibroblasts can acquire smooth muscle characteristics and that the acquisition of a smooth muscle-like phenotype correlates with increased contractility.
authors.affiliation: Department of Anatomical Sciences, University of Oklahoma Health Sciences Center, Oklahoma City, USA.
authors.forename: J
authors.initials: J
authors.lastname: Tomasek
authors.forename: G M
authors.initials: GM
authors.lastname: Rayan
mesh_terms: D000199:Actins / Q000096:biosynthesis*; D002349:Carpal Tunnel Syndrome / Q000601:surgery; D002478:Cells, Cultured; D003094:Collagen; D004387:Dupuytren Contracture / Q000378:metabolism*; D005205:Fascia; D005347:Fibroblasts / Q000737:chemistry* / Q000502:physiology; D005455:Fluorescent Antibody Technique; D006801:Humans; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000737:chemistry*
chemical_list: D000199:Actins; D003094:Collagen