Stimulation of cell proliferation and autoregulation of elastin expression by elastin peptide VPGVG in cultured chick vascular smooth muscle cells.
Synthetic elastin peptides, VPGVG or its polymer (VPGVG)n, enhanced the proliferation of smooth muscle cells 1.5-fold during 48 h treatment at the concentrations over 10(-6) M or 1.0 microgram/ml, respectively. Monomeric and polymeric VPGVG sequences reduced elastin synthesis and its mRNA level to one-third and one-half of control respectively under the conditions in which the proliferation of cells were enhanced, but did not change collagen synthesis as measured by bacterial collagenase digestion. The elastin-specific autoregulation by elastin fragments may reflect the feedback regulation of elastin expression which may play an essential role in elastin metabolism under the normal and diseased conditions.
Wachi
H
H
Department of Clinical Chemistry, Hoshi College of Pharmacy, Tokyo, Japan.
Seyama
Y
Y
Yamashita
S
S
Suganami
H
H
Uemura
Y
Y
Okamoto
K
K
Yamada
H
H
Tajima
S
S
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002645:Chickens; D003094:Collagen / Q000096:biosynthesis; D004549:Elastin / Q000096:biosynthesis*; D006706:Homeostasis / Q000187:drug effects*; D008969:Molecular Sequence Data; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D009842:Oligopeptides / Q000138:chemical synthesis / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis
D009842:Oligopeptides; D012333:RNA, Messenger; C094883:valyl-prolyl-glycyl-valyl-glycine; D003094:Collagen; D004549:Elastin; C042635:valyl-glycyl-valyl-alanyl-prolyl-glycine