title: Detection of human papillomavirus in oral lesions using commercially developed typing kits.
abstract: Biopsy material from 20 oral lesions (19 condylomas and 1 squamous papilloma) previously shown to contain human papillomavirus (HPV) 6 and HPV 11 sequences by in situ hybridization were examined using 3 commercially available HPV typing kits. Sensitivity and specificity were compared with in-house methods. Previous in situ hybridization had detected HPV 6b in 11 (55%) of the biopsies, HPV 6 and 11 in 7 (53%) and HPV 11 alone in 1 biopsy. Only one of the commercial assays (assay 1) detected HPV in all 20 biopsies (11 positive for HPV 6b only, 1 for HPV 11 only and 7 for HPV 6b and 11). The wide spectrum probe of assay 2 detected HPV in only 10 (50%) of the biopsies, and in a further 2 biopsies the hybridization results were difficult to interpret because of background staining. Assay 3 used a combined HPV 6/11 probe and detected HPV in 15 (75%) of the biopsies. Clear hybridization signals were demonstrated in the intermediate and upper layers only of squamous epithelium, as expected from the known association of HPV replication with epithelium differentiation. In most specimens background levels were not a problem, and all commercial assays were easy to use. The findings are discussed in the context of the digestion procedures, sensitivity of the probes provided and the conditions of hybridization, all of which would influence the detection of HPV.
authors.affiliation: Department of Oral Medicine and Oral Pathology, University of Edinburgh, United Kingdom.
authors.forename: S K
authors.initials: SK
authors.lastname: Dhariwal
authors.forename: H A
authors.initials: HA
authors.lastname: Cubie
authors.forename: J C
authors.initials: JC
authors.lastname: Southam
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003218:Condylomata Acuminata / Q000175:diagnosis / Q000821:virology*; D015344:DNA Probes, HPV; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008828:Microbiological Techniques; D008875:Middle Aged; D009059:Mouth Diseases / Q000175:diagnosis / Q000821:virology*; D009061:Mouth Mucosa / Q000821:virology; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000821:virology*; D010212:Papilloma / Q000175:diagnosis / Q000821:virology; D027383:Papillomaviridae / Q000145:classification / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014412:Tumor Virus Infections / Q000175:diagnosis
chemical_list: D015344:DNA Probes, HPV; D011933:Reagent Kits, Diagnostic