title: Mouse cytochrome P-450 linked ferredoxin: its cDNA cloning and inducibility by dibutyryladenosine 3',5'-cyclic monophosphate and forskolin.
abstract: Two full-length cDNAs (F1-1 and F41-1) complementary to mouse kidney mRNA coding for cytochrome P-450 (P450) linked ferredoxin were isolated and completely sequenced. The coding sequences between F1-1 and F41-1 were identical. However, the 3' untranslated regions of F1-1 and F41-1 were 228 and 27 bases long due to the presence of alternative polyadenylation sites, respectively. The deduced amino acid sequence of mouse cytochrome P-450 linked ferredoxin showed 92.5, 75.0, 71.2 and 71.0% identities with those of rat, human, pig and bovine cytochrome P-450 linked ferredoxin, respectively. The cytochrome P-450 linked ferredoxin mRNA was detected in adrenal, kidney and ovary among the organs examined. The treatment of Y-1 cells with dibutyryladenosine 3',5'-cyclic monophosphate or forskolin induced the transcript of cytochrome P-450 linked ferredoxin mRNA.
authors.affiliation: Division of Bio-Medical and Immunological Chemistry, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Itoh
authors.forename: O
authors.initials: O
authors.lastname: Iemura
authors.forename: E
authors.initials: E
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Yoshimura
authors.forename: K
authors.initials: K
authors.lastname: Tsujikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Kohama
authors.forename: T
authors.initials: T
authors.lastname: Mimura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D003001:Cloning, Molecular; D005576:Colforsin / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D004790:Enzyme Induction / Q000187:drug effects; D005288:Ferredoxins / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D051381:Rats
chemical_list: D018076:DNA, Complementary; D005288:Ferredoxins; D005576:Colforsin; D003994:Bucladesine; D003577:Cytochrome P-450 Enzyme System