Analysis of recombinant human growth hormone in Escherichia coli fermentation broth by micellar high-performance liquid chromatography.
A method for the reversed-phase high-performance liquid chromatographic (HPLC) determination of recombinant methionylaspartyl-human growth hormone (MD-HGH) in Escherichia coli fermentation broth is described. The technique utilizes mobile phases containing n-propanol and the anionic surfactant sodium dodecyl sulfate (SDS) under micellar conditions at pH 6.4. The methodology is directly applicable to the analysis of samples solubilized via sulfitolysis in the presence of SDS, and offers superior resolution in comparison with chromatography in the absence of the surfactant. Using this method, acceptable precision (day-to-day R.S.D. = 4.9%), accuracy, selectivity, range, linearity and ruggedness were achieved.
Strege
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Lilly Research Laboratories, Division of Eli Lilly and Co., Indianapolis, IN 46285, USA.
Lagu
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D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D005285:Fermentation; D013006:Growth Hormone / Q000032:analysis* / Q000235:genetics; D006801:Humans; D008823:Micelles; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics; D013056:Spectrophotometry, Ultraviolet
D008823:Micelles; D011994:Recombinant Proteins; D013006:Growth Hormone