Isotope effect studies on the cytochrome P450 enzymes.
Isotope effect experiments provide a powerful tool for study of the fundamental aspects of the enzymology of the cytochrome P450 enzymes. Competition between alternate pathways not only allows P450 isotope effects to be observed, but also provides mechanistic information on both oxygen activation and substrate oxidation. Indeed, the kind of knowledge that isotope effect studies can provide is not readily obtainable by other methodologies.
Korzekwa
K R
KR
Laboratory of Molecular Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Gillette
J R
JR
Trager
W F
WF
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003903:Deuterium / Q000378:metabolism; D007554:Isotopes; D009154:Mutation; D013250:Steroid Hydroxylases / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism*
D007554:Isotopes; D013739:Testosterone; D003577:Cytochrome P-450 Enzyme System; D003903:Deuterium; D013250:Steroid Hydroxylases; C022657:steroid hormone 6-beta-hydroxylase