Differential in situ hybridization for herpes simplex virus typing in routine skin biopsies.
A herpes simplex virus (HSV) type 2 specific recombinant plasmid probe designated pH2S3 was constructed from non-HSV-1 crossreactive regions of the HSV-2 genome. DNA in situ hybridization on in vitro reconstructed tissue samples of sheep collagen matrix impregnated with herpes virus-infected human cells was used to demonstrate absence of crossreactivity of the pH2S3 probe with HSV-1 and varicella zoster virus under stringent posthybridization washing conditions. It was demonstrated that DNA in situ hybridization with pH2S3 allows specific detection of HSV-2 in routine formalin-fixed, paraffin-embedded patient material.
Botma
H J
HJ
Department of Pathology-H2, University of Amsterdam, The Netherlands.
Dekker
H
H
van Amstel
P
P
Cairo
I
I
van den Berg
F M
FM
D000818:Animals; D001706:Biopsy; D002417:Cattle; D002478:Cells, Cultured; D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis; D006561:Herpes Simplex / Q000175:diagnosis / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000302:isolation & purification*; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000302:isolation & purification; D014645:Herpesvirus 3, Human / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D011092:Polyethylene Glycols; D012867:Skin / Q000473:pathology / Q000821:virology*
D015342:DNA Probes; D004279:DNA, Viral; D011092:Polyethylene Glycols