title: Phosphorylation and progesterone receptor function.
abstract: Four phosphorylation sites have been identified in the chicken progesterone receptor. Two of these sites exhibit basal phosphorylation which is enhanced upon treatment with hormone and two of the sites are phosphorylated in response to hormone. Mutation of one of these hormone dependent sites, Ser530 to Ala530, causes a decrease in transcriptional activation at low concentrations of hormone, but the activity is unaffected at high concentrations. However, the hormone binding of the mutant is unaffected suggesting that phosphorylation of Ser530 plays a role in facilitating the response of the receptor to low concentrations of hormone. The chicken progesterone receptor can be activated by modulators of kinases in the absence of hormone. The finding that signals initiated by tyrosine phosphorylation (through treatment with EGF) or through the dopamine receptor suggests that there are multiple means of activating chicken progesterone receptor. In contrast, the human progesterone receptor does not exhibit ligand independent activation; however, its activity in the presence of the agonist R5020 is enhanced by treatment with 8-Br-cAMP, an activator of protein kinase A, and treatment with 8-Br-cAMP causes the antagonist, RU486, to act as an agonist.
authors.affiliation: Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: N L
authors.initials: NL
authors.lastname: Weigel
authors.forename: W
authors.initials: W
authors.lastname: Bai
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: C A
authors.initials: CA
authors.lastname: Beck
authors.forename: D P
authors.initials: DP
authors.lastname: Edwards
authors.forename: A
authors.initials: A
authors.lastname: Poletti
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D010766:Phosphorylation; D011980:Receptors, Progesterone / Q000378:metabolism*; D015398:Signal Transduction; D013329:Structure-Activity Relationship
chemical_list: D008024:Ligands; D011980:Receptors, Progesterone