Persistent Epstein-Barr virus infection in a human T-cell line: unique program of latent virus expression.
The growth transforming potential of Epstein-Barr virus (EBV) for Burkitt's lymphoma and nasopharyngeal carcinoma is now extended to other neoplasia, such as Hodgkin's disease, peripheral T-cell tumor and gastric cancer. We have generated an EBV recombinant with a selectable marker at the viral thymidine kinase locus. Recombinant EBV was successfully infected into a human T-cell line, MT-2. Following incubation in the selective medium, drug resistant MT-2 cell clones were isolated and proved to be infected with recombinant EBV. EBV-infected MT-2 cell clones expressed EBNA 1 and LMP 1 and very little of EBNA 2, showing the BamHI F promoter-driven latency II form of infection, which is seen in non-B-cell tumors. This is the first report of in vitro generation of latency II type EBV infection. The present system of persistent EBV infection in T cells should be a good model for investigating the pathogenic role of EBV in non-B-cell tumors.
Yoshiyama
H
H
Department of Virology and Parasitology, Yamaguchi University School of Medicine, Japan.
Shimizu
N
N
Takada
K
K
D000956:Antigens, Viral / Q000096:biosynthesis; D001483:Base Sequence; D002460:Cell Line; D002461:Cell Line, Transformed; D004268:DNA-Binding Proteins / Q000096:biosynthesis; D019309:Epstein-Barr Virus Nuclear Antigens; D015967:Gene Expression Regulation, Viral; D005819:Genetic Markers / Q000235:genetics; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000502:physiology*; D015368:Human T-lymphotropic virus 1; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000821:virology*; D013937:Thymidine Kinase / Q000235:genetics; D014763:Viral Matrix Proteins / Q000096:biosynthesis; D017735:Virus Latency
D000956:Antigens, Viral; D004268:DNA-Binding Proteins; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D019309:Epstein-Barr Virus Nuclear Antigens; D005819:Genetic Markers; D012333:RNA, Messenger; D014763:Viral Matrix Proteins; D013937:Thymidine Kinase