Direct injection for high sample throughput capillary gas chromatographic-mass spectrometric bioanalysis.
Because of the drawback of the relatively long analysis times inherent to temperature-programmed splitless injection capillary GC-MS, isothermal direct injection capillary GC-MS was investigated for quantitative bioanalysis. Using extracts from spiked plasma samples, we showed that high quality chromatography with a run time much shorter than that achievable with splitless injection can be achieved with direct injection. Sensitivity and other performance parameters were as good as or better than those of the splitless method. Since sample throughput is of great importance in laboratories that analyze thousands of biological samples, it is recommended that, when possible, splitless injection, which has traditionally been used in trace level GC-MS bioanalytical methods, be replaced by direct injection.
Jemal
M
M
Bristol-Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543-4500, USA.
Patel
A
A
Teitz
D S
DS
D019086:Bridged Bicyclo Compounds, Heterocyclic; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D010080:Oxazoles / Q000097:blood / Q000493:pharmacokinetics; D010975:Platelet Aggregation Inhibitors / Q000097:blood / Q000493:pharmacokinetics; D011422:Propionates / Q000097:blood / Q000493:pharmacokinetics; D015203:Reproducibility of Results
D019086:Bridged Bicyclo Compounds, Heterocyclic; D010080:Oxazoles; D010975:Platelet Aggregation Inhibitors; D011422:Propionates; C078904:ifetroban