title: Quantitative competitive PCR with bronchoalveolar lavage fluid for diagnosis of toxoplasmosis in AIDS patients.
abstract: Of 121 AIDS patients, 12 (10%) had Toxoplasma gondii DNA detected by competitive PCR in their bronchoalveolar lavage samples. Quantitation of the PCR results showed a correlation between the parasite burden and the serum lactic dehydrogenase titer. Quantitative competitive PCR could be useful to assess the diagnosis and the severity of pulmonary toxoplasmosis.
authors.affiliation: Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor, Créteil, France.
authors.forename: S
authors.initials: S
authors.lastname: Bretagne
authors.forename: J M
authors.initials: JM
authors.lastname: Costa
authors.forename: J
authors.initials: J
authors.lastname: Fleury-Feith
authors.forename: F
authors.initials: F
authors.lastname: Poron
authors.forename: M L
authors.initials: ML
authors.lastname: Dubreuil-Lemaire
authors.forename: M
authors.initials: M
authors.lastname: Vidaud
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis* / Q000201:enzymology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000469:parasitology*; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008174:Lung Diseases, Parasitic / Q000150:complications / Q000175:diagnosis* / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000150:complications / Q000175:diagnosis* / Q000201:enzymology
chemical_list: D016054:DNA, Protozoan; D007770:L-Lactate Dehydrogenase