title: Expression of gicerin in development, oncogenesis and regeneration of the chick kidney.
abstract: Neurite outgrowth factor, which promotes neurite extension from neuronal cells, is an extracellular matrix glycoprotein belonging to the laminin family. Gicerin is a protein that binds neurite outgrowth factor. Its cDNA cloning has revealed that it is a novel cell adhesion molecule belonging to the immunoglobulin super-family. Functional analysis demonstrates that gicerin possesses homophilic binding activity as well as heterophilic binding activity with neurite outgrowth factor. We examined the role and expression of neurite outgrowth factor and gicerin in chick kidney during development. In the embryonic kidney, gicerin was found to be highly expressed both on ureteric bud cells and metanephrogenic mesenchymal cells, when the mesenchymal cells become condensed to be converted into polarized epithelial cells. In the adult kidney, the expression of gicerin was decreased and restricted to the glomerulus, proximal tubule and medullary loop. On the other hand, neurite outgrowth factor was constitutively expressed in the basement membranes of tubules and the matrices of glomeruli during development. As some molecules which are expressed during embryogenesis and suppressed after maturation are re-expressed in tumor cells or tissues during regeneration, we also examined the expression of gicerin in chicken Wilms' tumor and regenerating kidney in interstitial nephritis. Gicerin was remarkably upregulated in Wilms' tumor and re-expressed in collecting ducts recovering from interstitial nephritis. These findings suggest that gicerin could play a role not only in normal renal development but also in oncogenesis and regeneration.
authors.affiliation: Department of Pharmacology 1, Osaka University School of Medicine, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Takaha
authors.forename: E
authors.initials: E
authors.lastname: Taira
authors.forename: H
authors.initials: H
authors.lastname: Taniura
authors.forename: T
authors.initials: T
authors.lastname: Nagino
authors.forename: Y
authors.initials: Y
authors.lastname: Tsukamoto
authors.forename: T
authors.initials: T
authors.lastname: Matsumoto
authors.forename: T
authors.initials: T
authors.lastname: Kotani
authors.forename: S
authors.initials: S
authors.lastname: Sakuma
authors.forename: N
authors.initials: N
authors.lastname: Miki
mesh_terms: D000818:Animals; D030161:Avian Proteins; D015152:Blotting, Northern; D015153:Blotting, Western; D051929:CD146 Antigen; D002352:Carrier Proteins / Q000096:biosynthesis*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002642:Chick Embryo; D002645:Chickens; D005326:Fetal Proteins / Q000096:biosynthesis*; D007668:Kidney / Q000196:embryology / Q000254:growth & development / Q000502:physiology*; D007680:Kidney Neoplasms / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000096:biosynthesis; D012038:Regeneration / Q000502:physiology*; D009396:Wilms Tumor / Q000378:metabolism*
chemical_list: D030161:Avian Proteins; D051929:CD146 Antigen; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules; D005326:Fetal Proteins; D009414:Nerve Growth Factors; C086935:gicerin protein, Gallus gallus