Expression of 5-lipoxygenase in differentiating human skin keratinocytes.
We studied the expression of arachidonate 5-lipoxygenase (5-LO) in a cell line of human keratinocytes (HaCaT) and in normal human skin keratinocytes in tissue culture. In undifferentiated keratinocytes 5-LO gene expression was low or undetectable as determined by 5-LO mRNA, protein, cell-free enzyme activity, and leukotriene production in intact cells. However, after shift to culture conditions that promote conversion of prokeratinocytes into a more differentiated phenotype, 5-LO gene expression was markedly induced in HaCaT cells and, to a lesser extent, in normal keratinocytes. These results show that 5-LO gene expression is an intrinsic property of human skin keratinocytes.
Janssen-Timmen
U
U
Department of Internal Medicine, University of Heidelberg, Medical School, Germany.
Vickers
P J
PJ
Wittig
U
U
Lehmann
W D
WD
Stark
H J
HJ
Fusenig
N E
NE
Rosenbach
T
T
Rådmark
O
O
Samuelsson
B
B
Habenicht
A J
AJ
D001094:Arachidonate 5-Lipoxygenase / Q000096:biosynthesis* / Q000235:genetics; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis; D015603:Keratinocytes / Q000378:metabolism*; D007975:Leukotriene B4 / Q000096:biosynthesis; D017997:Leukotriene C4 / Q000096:biosynthesis; D008297:Male; D010413:Penis / Q000166:cytology; D012333:RNA, Messenger / Q000032:analysis; D012867:Skin / Q000166:cytology / Q000378:metabolism*
D006893:Hydroxyeicosatetraenoic Acids; D012333:RNA, Messenger; D007975:Leukotriene B4; D017997:Leukotriene C4; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; D001094:Arachidonate 5-Lipoxygenase