99mTc-ADP: a potential agent for in vivo tumor detection.
The possibility of using 99mTc-labelled nucleotides as tumour seeking agents has been proposed by different research groups. We have recently reported the preparation of a 99mTc-ADP complex with a high radiochemical purity (> 95%), good in vitro and in vivo stability and promising biodistribution results when injected in mice bearing spontaneous mammary adenocarcinomas. Here we report the results of further investigations in animals with spontaneous neoplastic processes, including whole-body autoradiography in mice (20 minutes and 60 minutes post injection) and gamma-camera imaging studies in Wistar rats. Dynamic studies (up to 45 minutes) and static images (up to 18 hours) were acquired to determine the pharmacokinetics of 99mTc-ADP and the tumour/muscle and tumour/blood ratios. Blood-pool studies were also performed as a control. Tumours were visualized by autoradiography as was to be expected from the biodistribution studies. Dynamic studies showed a rapid blood clearance and a behaviour that fitted to a tricompartimental model. Radioactivity was rapidly taken up by the kidneys and excreted in the urine. No evidence of in vivo instability of the complex was observed. Tumour uptake reached the maximum values after 20 minutes post-injection. Tumour/blood and tumour/muscle ratios improved over time, enhancing tumour visualization. The best images were obtained after 3 hours post injection. In summary, our studies suggest that 99mTc-ADP is a promising radiopharmaceutical for tumour diagnosis.
León
E T
ET
Cátedra de Radioquímica, Facultad de Química, Universidad de la República, Montevideo, Uruguay.
Rey
A M
AM
Savio
E O
EO
López
J J
JJ
Kremer
E
E
León
A S
AS
D000244:Adenosine Diphosphate / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D000818:Animals; D001345:Autoradiography; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000000981:diagnostic imaging; D051379:Mice; D008815:Mice, Inbred Strains; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics; D016719:Radioimmunodetection; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
D015609:Organotechnetium Compounds; C094944:technetium Tc 99m adenosine diphosphate; D000244:Adenosine Diphosphate