[Increase in the yield of recombinant proteins in Saccharomyces cerevisiae yeasts as a result of optimizing their culture conditions].
The optimal one-stage scheme of the cultivation of the recombinant yeast strains was developed. In these strains, the expression of the foreign gene is induced at the medium depletion by phosphorus. The scheme was tested for the producers of human interleukin-2 and bovine gamma-interferon. In all the strains, the yield of cell biomass and the production of the foreign proteins increased as compared to the standard two-stage cultivation. The use of the developed scheme resulted in the threefold increase in the yield of the target proteins and in the productivity of the biotechnological process.
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D000818:Animals; D001709:Biotechnology; D002417:Cattle; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D007376:Interleukin-2; D011994:Recombinant Proteins; D007371:Interferon-gamma
Povyshenie vykhoda rekombinantnykh belkov v drozhzhakh Saccharomyces cerevisiae v rezul'tate optimizatsii usloviĭ ikh kul'tivirovaniia.