title: Optimization of PCR-based diagnostics for human cytomegalovirus.
abstract: Various techniques of DNA template preparation for the PCR-based analysis of human CMV in biological fluids have been compared. Structural polymorphism of a CMV DNA segment (part of the major immediate early gene) in clinical isolates is described; the molecular markers (nucleotide substitutions, deletions, insertions) localized in the analyzed amplicon appear to be suitable for molecular-epidemiological studies. A scheme of spreading of the molecular markers in the population is suggested.
authors.affiliation: Centre of Molecular Diagnostics, St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences.
authors.forename: G R
authors.initials: GR
authors.lastname: Vinogradskaya
authors.lastname: Goryshin IYu
authors.lastname: Berlin YuA
authors.forename: V A
authors.initials: VA
authors.lastname: Lanzov
mesh_terms: D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000652:urine; D004279:DNA, Viral / Q000145:classification / Q000302:isolation & purification; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013698:Templates, Genetic
chemical_list: D004279:DNA, Viral