CD45 phosphatase in Jurkat cells is necessary for response to applied ELF magnetic fields.
Oscillations of free intracellular calcium [Ca2+]i were seen in individual Jurkat cells as response to a 50 Hz, 0.15 mT magnetic field (MF). In contrast, a CD45-deficient Jurkat cell line was unable to respond to MF stimulation. The phosphatase activity of CD45 has been implicated to regulate p56lck tyrosine kinase activity by removing an inhibitory phosphate. By using Jurkat cells that expressed a chimeric molecule, comprising the cytoplasmic phosphatase domain of CD45, the MF induced calcium response was restored. This showed the necessity for an intact signal transduction pathway leading to a calcium increase as a result of stimulation of cells by MF. Thus, our data suggest that the target for the applied MF are molecules involved in early events in the signalling pathway from the T cell antigen receptor.
Lindström
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Department of Cell and Molecular Biology, Umeå University, Sweden.
Berglund
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Mild
K H
KH
Lindström
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Lundgren
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D015703:Antigens, CD / Q000032:analysis / Q000096:biosynthesis* / Q000528:radiation effects; D002118:Calcium / Q000378:metabolism*; D002999:Clone Cells; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D008214:Lymphocytes; D008280:Magnetics; D009991:Oscillometry; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D013997:Time Factors; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D011505:Protein-Tyrosine Kinases; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck); D017027:Protein Tyrosine Phosphatases; D002118:Calcium