title: Differential scanning calorimetric study of the complexes of myosin subfragment 1 with nucleoside diphosphates and vanadate or beryllium fluoride.
abstract: It has been recently shown by differential scanning calorimetry (DSC) that the formation of stable complexes of myosin subfragment 1 (S1) with Mg-ADP and orthovanadate (Vi) or beryllium fluoride (BeFx) causes a global conformational change in the S1 molecule which is reflected in a pronounced increase of S1 thermal stability and in a significant change of S1 domain structure [Shriver, J. W., & Kamath U. (1990) Biochemistry 29, 2556-2564; Levitsky, D. I., Shnyrov, V. L., Khvorov, N. V., Bukatina, A. E., Vedenkina, N. S., Permyakov, E. A., Nikolaeva, O. P., & Poglazov, B. F. (1992) Eur. J. Biochem. 209, 829-835; Bobkov, A. A., Khvorov, N. V., Golitsina, N. L., & Levitsky, D. I. (1993) FEBS Lett. 332, 64-66]. In this work, which continues the previous investigations, we report on a DSC study of the complexes of S1 with various nucleoside diphosphates (NDP). In the absence of Vi or BeFx the various Mg(2+)-NDP and Mg(2+)-PPi had a similar effect on the S1 conformation. All of them had practically no influence on the temperature of the thermal transition but increased its sharpness. However, in the presence of Vi or BeFx the effects of Mg(2+)-NDP complexes were quite different from each other and strongly depended on the base structure of NDP; their effectiveness in inducing conformational changes in S1 and the stability of these complexes decreased in the following order: ADP > CDP >> UDP >> IDP > GDP.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: A. N. Bakh Institute of Biochemistry, Russian Academy of Sciences, Moscow.
authors.forename: A A
authors.initials: AA
authors.lastname: Bobkov
authors.forename: D I
authors.initials: DI
authors.lastname: Levitsky
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D001608:Beryllium / Q000378:metabolism*; D002152:Calorimetry, Differential Scanning; D003565:Cytidine Diphosphate / Q000494:pharmacology; D005459:Fluorides / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000494:pharmacology; D007290:Inosine Diphosphate / Q000494:pharmacology; D015879:Myosin Subfragments / Q000737:chemistry / Q000378:metabolism*; D009711:Nucleotides / Q000494:pharmacology*; D011487:Protein Conformation; D013816:Thermodynamics; D014530:Uridine Diphosphate / Q000494:pharmacology; D014638:Vanadates / Q000378:metabolism*
chemical_list: D015879:Myosin Subfragments; D009711:Nucleotides; D006153:Guanosine Diphosphate; D014638:Vanadates; C035982:beryllium fluoride; D014530:Uridine Diphosphate; D000244:Adenosine Diphosphate; D003565:Cytidine Diphosphate; D007290:Inosine Diphosphate; D001608:Beryllium; D005459:Fluorides