title: Characterization of recombinant tetanus toxin derivatives suitable for vaccine development.
abstract: Recombinant derivatives of tetanus toxin (TeTx) were isolated and used to immunize mice. Recombinant TeTx light chain, a derivative of fragment C that had lost the ability to bind neurons, and a recombinant TeTx holotoxoid that could protect mice against TeTx challenge were identified.
authors.affiliation: Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, United Kingdom.
authors.forename: D
authors.initials: D
authors.lastname: Figueiredo
authors.forename: C
authors.initials: C
authors.lastname: Turcotte
authors.forename: G
authors.initials: G
authors.lastname: Frankel
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: O
authors.initials: O
authors.lastname: Dolly
authors.forename: G
authors.initials: G
authors.lastname: Wilkin
authors.forename: D
authors.initials: D
authors.lastname: Marriott
authors.forename: N
authors.initials: N
authors.lastname: Fairweather
authors.forename: G
authors.initials: G
authors.lastname: Dougan
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis; D001428:Bacterial Vaccines / Q000276:immunology*; D005727:Ganglia, Spinal / Q000378:metabolism; D051379:Mice; D011485:Protein Binding; D051381:Rats; D011993:Recombinant Fusion Proteins; D013329:Structure-Activity Relationship; D013744:Tetanus Toxin / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D011993:Recombinant Fusion Proteins; D013744:Tetanus Toxin; D014614:Vaccines, Synthetic