Abnormalities of cAMP-dependent endogenous phosphorylation in platelets from patients with bipolar disorder.
OBJECTIVE
The aim of the study was to assess cAMP-dependent endogenous phosphorylation in platelets from euthymic bipolar patients.


METHODS
Platelets from 10 drug-free euthymic patients with bipolar disorder were compared with those from 10 age- and sex-matched healthy subjects. Basal and cAMP-stimulated protein phosphorylation was examined in each group.


RESULTS
Endogenous phosphorylation in both the healthy volunteers and the bipolar patients was significantly stimulated by cAMP; the major polypeptides had apparent molecular weights of 38 and 22 kDa. The cAMP-stimulated 32P incorporation differed between the bipolar patients and the comparison subjects only in the 22-kDa band.


CONCLUSIONS
These data suggest a possible role of cAMP-dependent protein phosphorylation in the pathophysiology of bipolar disorder.
Perez
J
J
Center for Neuropharmacology, Institute of Pharmacological Sciences, University of Milan, Italy.
Zanardi
R
R
Mori
S
S
Gasperini
M
M
Smeraldi
E
E
Racagni
G
G
D000328:Adult; D015415:Biomarkers; D001714:Bipolar Disorder / Q000097:blood* / Q000503:physiopathology; D001792:Blood Platelets / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D006801:Humans; D008297:Male; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects
D015415:Biomarkers; D010761:Phosphorus Radioisotopes; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases