A role for calcitonin gene-related peptide in the regulation of rat skeletal muscle G4 acetylcholinesterase.
Calcitonin gene-related peptide (CGRP) acts as an anterograde trophic agent which regulates skeletal muscle acetylcholine receptor function. We examined whether CGRP also influences other synaptic transmission-related molecules, i.e. acetylcholinesterase (AChE) forms. Results show that: (a) CGRP associated with rat anterior gracilis muscle endplates declines following obturator nerve transection; (b) exogenous CGRP treatment has a selective, innervation-like effect on the globular tetramer (G4) of AChE in gracilis motor endplates; and (c) this effect is reversed by the CGRP receptor antagonist hCGRP8-37. We conclude that exogenous CGRP, and/or a biologically active CGRP fragment(s), influences G4 AChE levels through specific CGRP-CGRP receptor interactions. This conclusion is consistent with the notion that motor nerve-derived CGRP participates in the trophic control of G4 AChE at the neuromuscular junction.
Hodges-Savola
C A
CA
Molecular Neurobiology Laboratory, Department of Veterans Affairs Medical Center, Minneapolis, MN 55417, USA.
Fernandez
H L
HL
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism / Q000502:physiology*; D008297:Male; D009045:Motor Endplate / Q000201:enzymology; D009121:Muscle Denervation; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000378:metabolism; D009469:Neuromuscular Junction / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000378:metabolism; D009435:Synaptic Transmission / Q000187:drug effects
D018015:Receptors, Calcitonin Gene-Related Peptide; D000110:Acetylcholinesterase; D015740:Calcitonin Gene-Related Peptide