Separation of oviposition-stimulating peptides and myotropic factors from head extracts of Galleria mellonella L.: comparative effects of myotropic and non-myotropic factors on egg laying.
Methanolic extracts from heads of the wax moth, Galleria mellonella L. contain several factors that stimulate oviposition of virgin females in vivo and spontaneous contractions of the oviduct in vitro of the cricket, Gryllus bimaculatus. The myotropic and egg-laying activities behaved distinctly during all steps of purification, including fractionation on reverse-phase high-performance liquid chromatography. A novel neuropeptide was isolated from Galleria mellonella. The peptide does not increase the motility of isolated cricket oviducts. Among 13 other myotropic or non-myotropic factors studied, none were found to be as potent as the new peptide in stimulating egg-laying activity. The dose-response curves of myotropic and non-myotropic factors indicate that (i) proctolin, L-glutamate, octopamine, leucokinin-VI, leucopyrokinin, ecdysone and 20-hydroxy-ecdysone, or equivalent structures, might be involved in Galleria mellonella oviposition control, and (ii) that only the novel wax moth neuropeptide has the ability to trigger egg laying in that insect. The significance of these findings is discussed.
Abdoun
K
K
Laboratoire de Bioactivation des Peptides, Institut Jacques-Monod, Paris, France.
Mesnier-Sabin
M
M
Baudry-Partiaoglou
N
N
Nicolas
P
P
Cohen
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry / Q000502:physiology; D002457:Cell Extracts / Q000302:isolation & purification / Q000494:pharmacology; D004440:Ecdysone / Q000494:pharmacology; D004441:Ecdysterone / Q000494:pharmacology; D005260:Female; D018698:Glutamic Acid / Q000494:pharmacology; D006135:Gryllidae / Q000502:physiology*; D007301:Insect Hormones / Q000737:chemistry / Q000494:pharmacology; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry / Q000502:physiology*; D009479:Neuropeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000737:chemistry / Q000494:pharmacology; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D010057:Oviducts / Q000187:drug effects / Q000502:physiology; D010058:Oviposition / Q000187:drug effects* / Q000502:physiology; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives
D002457:Cell Extracts; D007301:Insect Hormones; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D009842:Oligopeptides; C049557:leucopyrokinin; D004440:Ecdysone; D018698:Glutamic Acid; D004441:Ecdysterone; C011536:proctolin; D011761:Pyrrolidonecarboxylic Acid