title: Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus.
abstract: Mutants of the extreme thermophile Thermus flavus in the pyrimidine biosynthetic pathway (Pyr-) were isolated by resistance to 5-fluoroorotic acid. The pyrE gene, which codes for the orotate phosphoribosyltransferase, was cloned by recombination with one of the isolated Pyr- T. flavus mutant strains. It was subcloned by complementation of an Escherichia coli pyrE mutant strain and was sequenced. The deduced polypeptide sequence extends over 183 amino acids. Several independent Pyr- mutations were mapped within the pyrE locus by recombination with fragments of the cloned gene.
authors.affiliation: ThermoGen, Inc., Chicago, Illinois 60612, USA.
authors.forename: V
authors.initials: V
authors.lastname: Vonstein
authors.forename: S P
authors.initials: SP
authors.lastname: Johnson
authors.forename: H
authors.initials: H
authors.lastname: Yu
authors.forename: M J
authors.initials: MJ
authors.lastname: Casadaban
authors.forename: N C
authors.initials: NC
authors.lastname: Pagratis
authors.forename: J M
authors.initials: JM
authors.lastname: Weber
authors.forename: D C
authors.initials: DC
authors.lastname: Demirjian
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D009962:Orotate Phosphoribosyltransferase / Q000096:biosynthesis / Q000235:genetics; D013824:Thermus / Q000201:enzymology / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D009962:Orotate Phosphoribosyltransferase