title: Receptor binding site-deleted foot-and-mouth disease (FMD) virus protects cattle from FMD.
abstract: Binding of foot-and-mouth disease virus (FMDV) to cells requires an arginine-glycine-aspartic acid (RGD) sequence in the capsid protein VP1. We have genetically engineered an FMDV in which these three amino acids have been deleted, producing a virus particle which is unable to bind to cells. Cattle vaccinated with these receptor binding site-deleted virions were protected from disease when challenged with a virulent virus, demonstrating that these RGD-deleted viruses could serve as the basis for foot-and-mouth disease vaccines safer than those currently in use. This strategy may prove useful in the development of vaccines for other viral diseases.
authors.affiliation: Plum Island Animal Disease Center, U.S. Department of Agriculture, Greenport, New York 11944, USA.
authors.forename: T S
authors.initials: TS
authors.lastname: McKenna
authors.forename: J
authors.initials: J
authors.lastname: Lubroth
authors.forename: E
authors.initials: E
authors.lastname: Rieder
authors.forename: B
authors.initials: B
authors.lastname: Baxt
authors.forename: P W
authors.initials: PW
authors.lastname: Mason
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005537:Aphthovirus / Q000235:genetics / Q000276:immunology*; D001665:Binding Sites; D002417:Cattle; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control; D008969:Molecular Sequence Data; D009842:Oligopeptides; D011991:Receptors, Virus / Q000502:physiology*; D017384:Sequence Deletion; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D014771:Virion / Q000235:genetics / Q000276:immunology
chemical_list: D009842:Oligopeptides; D011991:Receptors, Virus; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; C047981:arginyl-glycyl-aspartic acid