Quantitative competitive PCR with bronchoalveolar lavage fluid for diagnosis of toxoplasmosis in AIDS patients.
Of 121 AIDS patients, 12 (10%) had Toxoplasma gondii DNA detected by competitive PCR in their bronchoalveolar lavage samples. Quantitation of the PCR results showed a correlation between the parasite burden and the serum lactic dehydrogenase titer. Quantitative competitive PCR could be useful to assess the diagnosis and the severity of pulmonary toxoplasmosis.
Bretagne
S
S
Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor, Créteil, France.
Costa
J M
JM
Fleury-Feith
J
J
Poron
F
F
Dubreuil-Lemaire
M L
ML
Vidaud
M
M
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis* / Q000201:enzymology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000469:parasitology*; D016054:DNA, Protozoan / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008174:Lung Diseases, Parasitic / Q000150:complications / Q000175:diagnosis* / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D014122:Toxoplasma / Q000235:genetics / Q000302:isolation & purification; D014123:Toxoplasmosis / Q000150:complications / Q000175:diagnosis* / Q000201:enzymology
D016054:DNA, Protozoan; D007770:L-Lactate Dehydrogenase