The first nucleotide sequence of an archaeal elongation factor 1 beta gene.
An archaeal elongation factor 1 beta gene has been isolated for the first time from a Sulfolobus solfataricus genomic library. The sequenced clone (869 bp) contained two open reading frames, one coding for a protein made of 91 amino acid residues (SsEF-1 beta), the other one encoding a nonidentified product (ORF 115). The amino acid sequences of segments at the N- and C-terminal of the translated SsEF-1 beta were identical to those determined for the native protein. Northern and Southern analyses showed that the SsEF-1 beta gene is represented in S. solfataricus by a unique sequence. Compared to eubacterial or eukaryal corresponding genes the SsEF-1 beta is much shorter.
Arcari
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P
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Italy.
Raimo
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G
Ianniciello
G
G
Gallo
M
M
Bocchini
V
V
D000595:Amino Acid Sequence; D001483:Base Sequence; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000737:chemistry / Q000235:genetics*; D016944:Sulfolobus / Q000235:genetics*
D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors