title: Morphological analysis of ileal grafting following ileocystoplasty in the rat: a kinetic and ultrastructural study of the intestinal epithelium.
abstract: Ileocystoplasty was performed in rats and the morphological and cell-kinetic changes occurring in the ileal grafts were determined at intervals up to 18 months postoperatively. The intestinal mucosa underwent no progressive changes but included villous and avillous regions associated with crypts of various sizes at all time intervals. Newly appearing and densely packed epithelial cells, shaped like petals, were always present in the lower parts of the villi associated with crypts showing no elongation, but seldom present in those with elongated crypts in the villous mucosa. Bromodeoxyuridine studies showed that the petal-shaped cells interfered with cell migration. No petal-shaped cells were observed in avillous mucosa in which the rate of cell turnover depended on crypt size. Fine-structural changes in absorptive epithelial cells in both types of mucosa included features of prematurity or hypermaturity in the cytoplasm and close adherence to the basal portions of adjacent cells and to the basal lamina. These changes may possibly contribute to the prevention of reabsorption of urine. However, some of the mechanisms responsible for adherence of the basal parts might incidentally interfere with the normal cell kinetics of the intestinal epithelium, resulting in dense packing of cells and the formation of multiple types of mucosa in ileal grafts.
authors.affiliation: Department of Urology, Shiga University of Medical Science, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Makiura
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: T
authors.initials: T
authors.lastname: Tomoyoshi
authors.forename: T
authors.initials: T
authors.lastname: Maeda
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001973:Bromodeoxyuridine; D002463:Cell Membrane Permeability; D004848:Epithelium / Q000378:metabolism / Q000648:ultrastructure; D006085:Graft Survival; D007082:Ileum / Q000637:transplantation* / Q000648:ultrastructure; D007413:Intestinal Mucosa / Q000648:ultrastructure*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen; D051381:Rats; D017208:Rats, Wistar; D001743:Urinary Bladder / Q000601:surgery*
chemical_list: D000911:Antibodies, Monoclonal; D018809:Proliferating Cell Nuclear Antigen; D001973:Bromodeoxyuridine