Cloning and characterisation of a bovine P2Y receptor.
Using a chick P2Y1 receptor cDNA probe we have isolated a mammalian P2Y receptor clone from a bovine aortic endothelial cell library. The sequence has a high degree of similarity to the chick P2Y1 clone. When transfected into the Jurkat cell line, the cDNA conferred sensitivity to purinoceptor agonists. Using fura-2 loaded cells the potency order at the receptor was found to be 2-methylthioadenosine 5' triphosphate = adenosine 5' diphosphate > adenosine 5' triphosphate >> alpha,beta-methyleneadenosine 5' triphosphate and uridine 5' triphosphate. This corresponds to the agonist potency order expected for the bovine aortic endothelial cell P2Y receptor.
Henderson
D J
DJ
Molecular Neurobiology Unit, Royal Free Hospital School of Medicine, London, UK.
Elliot
D G
DG
Smith
G M
GM
Webb
T E
TE
Dainty
I A
IA
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001011:Aorta / Q000737:chemistry; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004730:Endothelium, Vascular / Q000737:chemistry; D016257:Fura-2; D008969:Molecular Sequence Data; D018048:Receptors, Purinergic P2 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017385:Sequence Homology; D014162:Transfection; D014544:Uridine Triphosphate / Q000494:pharmacology
D018076:DNA, Complementary; D018048:Receptors, Purinergic P2; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D002118:Calcium; D016257:Fura-2; D014544:Uridine Triphosphate