Optimization of PCR-based diagnostics for human cytomegalovirus.
Various techniques of DNA template preparation for the PCR-based analysis of human CMV in biological fluids have been compared. Structural polymorphism of a CMV DNA segment (part of the major immediate early gene) in clinical isolates is described; the molecular markers (nucleotide substitutions, deletions, insertions) localized in the analyzed amplicon appear to be suitable for molecular-epidemiological studies. A scheme of spreading of the molecular markers in the population is suggested.
Vinogradskaya
G R
GR
Centre of Molecular Diagnostics, St. Petersburg Institute of Nuclear Physics, Russian Academy of Sciences.
Goryshin IYu
Berlin YuA
Lanzov
V A
VA
D001483:Base Sequence; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000652:urine; D004279:DNA, Viral / Q000145:classification / Q000302:isolation & purification; D006801:Humans; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013698:Templates, Genetic
D004279:DNA, Viral