Cloning of boar SPMI gene which is expressed specifically in seminal vesicle and codes for a sperm motility inhibitor protein.
Boar semen contains a seminal plasma motility inhibitor (SPMI) that blocks the motility of demembranated-reactivated spermatozoa as well as of intact spermatozoa. In this paper, we describe the primary structure of SPMI, the coding of boar SPMI cDNA gene and its expression in various porcine tissues. Nucleotide sequence analysis of the 645-bp SPMI cDNA predicts a coded polypeptide of 137 amino acid residues which includes a 21-residue signal peptide and a 116-residue secreted protein. The amino acid sequence of SPMI was found to be highly homologous to AQN-3, a member of spermadhesin family proteins of boar that bind to spermatozoa. Expression of the boar SPMI gene detected by Northern blot analysis revealed that its expression is very abundant in seminal vesicles and specific to this tissue.
Iwamoto
T
T
Department of Urology, St Marianna University School of Medicine, Kawasaki, Japan.
Hiroaki
H
H
Furuichi
Y
Y
Wada
K
K
Satoh
M
M
Satoh
M
M
Osada
T
T
Gagnon
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D008297:Male; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D029607:Seminal Vesicle Secretory Proteins; D012669:Seminal Vesicles / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013081:Sperm Motility / Q000502:physiology*; D013552:Swine
D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; D029607:Seminal Vesicle Secretory Proteins; C424482:seminal vesicle-specific antigen