T-cell, adhesion, and B-cell epitopes of the cell surface Streptococcus mutans protein antigen I/II.
The T-cell and antibody responses to a cell surface streptococcal antigen (SA I/II) were investigated in naturally sensitized humans. Serum antibody responses were directed predominantly to the N-terminal (residues 39 to 481) and central (residues 816 to 1213) regions of SA I/II which may be involved in bacterial adhesion to salivary receptors. T-cell responses were also directed predominantly towards the central region. The linear peptide relationship of the immunodominant and minor T- and B-cell as well as adhesion epitopes was mapped within residues 816 to 1213. Immunodominant T-cell and B-cell epitopes were identified within residues 803 to 853, which were separated in linear sequence from the adhesion epitopes (residues 1005 to 1044). Adhesion epitopes overlapped with minor B- and T-cell epitopes (residues 1005 to 1054 and 1085 to 1134). An immunodominant promiscuous T-cell epitope (residues 985 to 1004) was adjacent to an adhesion epitope (residues 1005 to 1024). The limited B-cell response to adhesion epitopes is consistent with the success of Streptococcus mutans in colonizing the oral cavity. The strategy of T-cell, adhesion, and B-cell epitope mapping has revealed a general approach for identifying components of subunit vaccines which may focus responses to critical functional determinants. Such epitopes of SA I/II may constitute the components of a subunit vaccine against dental caries.
Kelly
C G
CG
Department of Immunology, United Medical School at Guy's Hospital, London, United Kingdom.
Todryk
S
S
Kendal
H L
HL
Munro
G H
GH
Lehner
T
T
D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001422:Bacterial Adhesion; D001483:Base Sequence; D003731:Dental Caries / Q000276:immunology; D018604:Epitope Mapping; D006801:Humans; D008213:Lymphocyte Activation; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D013295:Streptococcus mutans / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D011994:Recombinant Proteins