Action of retinoic acid on human glioblastoma-astrocytoma--14 cells in culture.
Monolayer and agar culture techniques were used to examine the antiproliferative activities and morphological alterations of glioblastoma-astrocytoma (G1-As-14) cells induced by 20 mumol retinoic acid (RA). RA treated cells assumed flattened appearance and formed multilayers no longer. Most of the cells formed cross-bridges with one another. RA treatment caused time-dependent, dose-dependent and cell seeding-dependent reduction of growth in both monolayer and in agar cultures. RA-induced growth inhibition was also affected by concentration of fetal bovine serum in the culture medium. All these effects could be reversed within 48 h after withdrawal of RA from the growth medium. The results demonstrated that the respective cell line was sensitive to RA-induced growth inhibition and morphological alterations which were generally associated with reduced expression of malignant phenotype.
Mukherjee
P
P
Department of Tissue Culture, Chittaranjan National Cancer Institute, Calcutta, India.
Das
S K
SK
D001254:Astrocytoma / Q000188:drug therapy* / Q000473:pathology; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D005909:Glioblastoma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D003470:Culture Media; D014212:Tretinoin