title: Interleukin-10 messenger ribonucleic acid in human placenta: implications of a role for interleukin-10 in fetal allograft protection.
abstract: OBJECTIVE
  Our purpose was to determine whether interleukin-10 is expressed in human placental tissue, which might imply a role for it in fetal allograft protection.
  
  
  METHODS
  Detection of interleukin-10 messenger ribonucleic acid in human placental tissue and in human placental JAR cells by reverse transcription-coupled polymerase chain reaction was studied.
  
  
  RESULTS
  Interleukin-10 messenger ribonucleic acid was detected in human placental tissue from term mothers and in human placental JAR cells. Sequence analysis of the expected interleukin-10 complementary deoxyribonucleic acid fragment revealed 100% homology to authentic interleukin-10 complementary deoxyribonucleic acid.
  
  
  CONCLUSIONS
  Our results indicated that human placental tissue from term mothers expressed high levels of interleukin-10 messenger ribonucleic acid, suggesting that cells that produce interleukin-10 and that are associated with the placenta may play a role in preventing rejection of the fetal allograft by the mother.
authors.affiliation: Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston 77555-1019, USA.
authors.forename: P
authors.initials: P
authors.lastname: Cadet
authors.forename: P L
authors.initials: PL
authors.lastname: Rady
authors.forename: S K
authors.initials: SK
authors.lastname: Tyring
authors.forename: R B
authors.initials: RB
authors.lastname: Yandell
authors.forename: T K
authors.initials: TK
authors.lastname: Hughes
mesh_terms: D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis; D005260:Female; D005333:Fetus / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000502:physiology*; D008969:Molecular Sequence Data; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis*; D014184:Transplantation, Homologous
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D016753:Interleukin-10