title: Screening method for cellulose biosynthesis inhibitors with herbicidal activity.
abstract: A new screening method for inhibitors of cellulose biosynthesis is described. This method utilized three microbial strains; a cellulose-containing fungus Phytophthora, and a cellulose non-containing fungus Candida, and a bacterial strain of Acetobacter, a cellulose-producing acetic acid bacterium. The primary screen examined microbial cultures for selective growth inhibition against Phytophthora with no inhibition against Candida. The secondary screen tested for herbicidal activity. Thirdly, the active cultures were examined for their inhibition of cellulose biosynthesis by an Acetobacter strain. A screening trial with this new method led to the discovery of two microbial metabolites named phthoxazolin A and phthoramycin as new inhibitors of cellulose biosynthesis with herbicidal activity.
authors.affiliation: Research Center for Biological Function, Kitasato Institute, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Sugoh
authors.forename: W
authors.initials: W
authors.lastname: Ji
authors.forename: J
authors.initials: J
authors.lastname: Iwabuchi
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: S
authors.initials: S
authors.lastname: Omura
mesh_terms: D000091:Acetobacter / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects; D002482:Cellulose / Q000096:biosynthesis*; D005233:Fatty Alcohols / Q000494:pharmacology*; D006540:Herbicides / Q000494:pharmacology*; D018942:Macrolides; D008826:Microbial Sensitivity Tests; D010080:Oxazoles / Q000494:pharmacology*; D010838:Phytophthora / Q000187:drug effects / Q000254:growth & development; D053284:Polyunsaturated Alkamides; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D005233:Fatty Alcohols; D006540:Herbicides; D018942:Macrolides; D010080:Oxazoles; D053284:Polyunsaturated Alkamides; C058284:phthoramycin; C066102:phthoxazolin; D002482:Cellulose