Pattern formation on cardiac troponin I by consecutive phosphorylation and dephosphorylation.
Two serine residues located adjacently in the heart-specific N-terminus of cardiac troponin I can be phosphorylated in vivo. Both residues are sequentially phosphorylated and dephosphorylated by cAMP-dependent protein kinase (PKA) and protein phosphatase 2A (PP2A). The concentration changes of the different troponin I species have been determined separately for the phosphorylation and dephosphorylation reaction and approximated by time courses predicted by a reaction model. Dependent on the concentration ratio of active protein kinase/protein phosphatase, four different troponin I species can be generated; one nonphosphorylated, two monophosphorylated and one bisphosphorylated. This pattern generation will be observed in proteins phosphorylated and dephosphorylated by a single protein kinase and phosphatase on more than one site and is a new principle inherent in signal cascades.
Jaquet
K
K
Krankenhausbetriebsgesellschaft Bad Oeynhausen mbH, Herzzentrum Nordrhein-Westfalen, Germany.
Thieleczek
R
R
Heilmeyer
L M
LM
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D003545:Cysteine / Q000378:metabolism; D007700:Kinetics; D008969:Molecular Sequence Data; D009206:Myocardium / Q000737:chemistry / Q000201:enzymology; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation; D010768:Phosphoserine / Q000378:metabolism; D054648:Protein Phosphatase 2; D012694:Serine / Q000378:metabolism; D014336:Troponin / Q000378:metabolism*; D019210:Troponin I
D010446:Peptide Fragments; D014336:Troponin; D019210:Troponin I; D010768:Phosphoserine; D012694:Serine; D017868:Cyclic AMP-Dependent Protein Kinases; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2; D003545:Cysteine