title: Evaluation of extracellular matrices and the plasminogen activator system in sheep inner cell mass and trophectodermal outgrowth in vitro.
abstract: Effects of extracellular matrices (ECM) and the plasminogen activator (PA) system on outgrowth of sheep inner cell masses (ICM) and trophectoderm in vitro were investigated. Experiment 1 evaluated the effects of plasminogen and ECM type on ICM and trophectodermal outgrowth, on glass Lab-Tek chamber slides coated with collagen IV, fibronectin, or laminin. ICM outgrowth areas were reduced (p < 0.05) by plasminogen and were greatest (p < 0.05) on fibronectin. Trophectodermal outgrowth was not supported in this system. Experiment 2 evaluated the effects of PA inhibitor-2 (PAI-2) or antiserum to urokinase-type PA (anti-uPA) on ICM outgrowth on fibronectin. Numbers of cells in the outgrowths were increased (p < 0.05) with PAI-2, and anti-uPA had no effect (p > 0.10). Experiment 3 evaluated the relationship between PA production and ECM type on ICM and trophectodermal outgrowth in microdrop cultures. PA production by ICM was greatest (p < 0.05) on fibronectin, but no differences (p > 0.10) were observed for trophectoderm. PA production was not correlated with ICM outgrowth areas (r = -0.12; p = 0.72) or numbers of cells in the ICM outgrowths (r = 0.09; p = 0.74) but was correlated with ICM areas (r = 0.75; p < 0.01) and numbers of cells in trophectodermal outgrowths (r = 0.57; p = 0.01). These results suggest that type of ECM, culture system, and alterations in the PA system influence cellular outgrowths by ICM and trophectoderm.
authors.affiliation: Department of Animal Sciences, Oregon State University, Corvallis 97331-6702, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Bartlett
authors.forename: A R
authors.initials: AR
authors.lastname: Menino
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D002465:Cell Movement; D003094:Collagen; D003470:Culture Media; D017077:Culture Media, Conditioned; D046508:Culture Techniques; D004475:Ectoderm / Q000166:cytology / Q000502:physiology*; D005109:Extracellular Matrix / Q000502:physiology*; D005260:Female; D005353:Fibronectins; D007106:Immune Sera / Q000494:pharmacology; D007797:Laminin; D010958:Plasminogen / Q000494:pharmacology; D017396:Plasminogen Activator Inhibitor 2 / Q000494:pharmacology; D010960:Plasminogen Activators / Q000502:physiology*; D012756:Sheep / Q000502:physiology*; D014568:Urokinase-Type Plasminogen Activator / Q000276:immunology / Q000502:physiology
chemical_list: D003470:Culture Media; D017077:Culture Media, Conditioned; D005353:Fibronectins; D007106:Immune Sera; D007797:Laminin; D017396:Plasminogen Activator Inhibitor 2; D010958:Plasminogen; D003094:Collagen; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator