High-molecular-size mite allergens with high carbohydrate content in mite extract containing mite feces of Dermatophagoides farinae.
A series of mite allergens with high carbohydrate content was fractionated from mite extract containing mite feces by ultrafiltration, and successive chromatography on Ultrogel AcA 54, Sepharose CL-6B, and DEAE Toyopearl 650S. The final fractions isolated were termed HM1-2-Eff, -0.1A, -0.1B, -0.3A, -0.3B, and -1M according to the eluted salt concentration. These six fractions were stained weakly with Coomassie Brilliant Blue but strongly with periodate-Schiff's reagent on SDS-PAGE. Their molecular weight were estimated to be 150-155 KD on Sepharose CL-6B. All of these six fractions provoked strong erythema in skin of mite-sensitive and asthmatic patients. HM1-2-Eff, -0.1A, -0.1B and -0.3B reacted with 73% of mite-sensitive patients' IgE by the nitrocellulose dot blotting. One ng/ml of 4 fractions (HM1-2-0.1A, 0.3A, 0.3B and -1M) released histamine from mite allergic patient's blood cells. HM1-2-0.3A induced proliferation of T cell from immunized mouse by mite extract containing mite feces (Dff).
Shigeta
S
S
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University.
Tanaka
S
S
Yoshizane
T
T
Yamaguchi
T
T
Fujii
S
S
Jyo
T
T
Wada
T
T
Oka
S
S
Ono
K
K
D000485:Allergens / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D001249:Asthma / Q000276:immunology; D002241:Carbohydrates / Q000032:analysis*; D005243:Feces / Q000737:chemistry*; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008925:Mites / Q000737:chemistry / Q000276:immunology*; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000276:immunology
D000485:Allergens; D002241:Carbohydrates; D007074:Immunoglobulin G; D007073:Immunoglobulin E