Experimental leprosy in monkeys. II. Longitudinal serological observations in sooty mangabey monkeys.
In this study, 11 SMM were grouped and inoculated with differing doses of SMM-origin Mycobacterium leprae (ML) between 4.5 x 10(8) and 1 x 10(9) by either combined IV/IC routes or by IV or IC route alone. The combined route was the most effective in eliciting progressive, disseminated LL leprosy. In all, 6 of 7 SMM inoculated by the combined routes developed leprosy requiring treatment at some point. Only 1 of 4 inoculated by a single route developed persisting leprosy requiring chemotherapy. Either no disease or spontaneous regression of initial disease occurred in the other 3 animals inoculated by a single route. Doses in excess of 1 x 10(9) ML were more effective than lesser doses. An association was observed between the development of IgG anti-PGL-I ELISA OD values and resistance to leprosy and between IgM anti-PGL-I and leprosy progression or susceptibility. Serum PGL-I antigen levels, determined by dot ELISA, paralleled disease severity longitudinally. High positive OD values of anti-LAM IgG prior to ML inoculation were observed in the majority of leprosy-susceptible SMM in contrast to negative levels in more resistant animals. Anti-LAM IgG OD values exceeded the positive cut-off point after inoculation in 5 of 11 SMM; 3 of these 5 had concurrent detectable serum levels of PGL-I antigen.
Gormus
B J
BJ
Department of Microbiology, Tulane Regional Primate Research Center, Covington, LA, USA.
Xu
K
K
Cho
S N
SN
Baskin
G B
GB
Bohm
R P
RP
Martin
L N
LN
Blanchard
J L
JL
Mack
P A
PA
Ratterree
M S
MS
Meyers
W M
WM
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D016665:Cercocebus atys; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007918:Leprosy / Q000276:immunology*; D008070:Lipopolysaccharides / Q000032:analysis*; D008137:Longitudinal Studies; D009166:Mycobacterium leprae / Q000276:immunology*
D000942:Antigens, Bacterial; D006017:Glycolipids; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; C050016:lipoarabinomannan; C045169:phenolic glycolipid I, Mycobacterium leprae