title: Structure-function correlations in cryopreserved allograft cardiac valves.
abstract: This communication briefly summarizes a morphologic investigation of explanted cryopreserved heart valves and discusses the findings in the context of ongoing debates regarding modes of failure, cellular viability, durability of the extracellular matrix, and the contribution of immune responses. We studied 20 cryopreserved human heart valve allografts functioning up to 9 years as either orthotopic aortic valves/root replacements or right ventricle-to-pulmonary artery conduits explanted for valve failure, infection, or growth-related conduit or valve stenosis. Implanted valves had progressively severe loss of normal layered structure and were devoid of stainable deep connective tissue cells. Inflammation was minimal. Other late findings included minimal inflammation, mild cuspal hematomas, mural thrombus, and calcification, most prominent in the aortic wall. Transmission electron microscopy of late explants revealed nonviable cells and their debris, and a collagenous skeleton that was largely intact. We conclude that cryopreserved allograft heart valves have minimal, if any, viable cells, but largely retain the original collagen network; preservation of the autolysis-resistant collagenous skeleton likely provides the structural basis of function. Our results also suggest that immune responsiveness has little, if any, impact on late allograft function or degradation.
authors.affiliation: Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA.
authors.forename: R N
authors.initials: RN
authors.lastname: Mitchell
authors.forename: R A
authors.initials: RA
authors.lastname: Jonas
authors.forename: F J
authors.initials: FJ
authors.lastname: Schoen
mesh_terms: D000293:Adolescent; D000328:Adult; D002470:Cell Survival; D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation; D005109:Extracellular Matrix / Q000473:pathology; D006085:Graft Survival; D006351:Heart Valves / Q000473:pathology / Q000637:transplantation*; D006801:Humans; D008875:Middle Aged; D014181:Transplantation Immunology; D014184:Transplantation, Homologous / Q000276:immunology