Restriction endonuclease cleavage analysis of herpes simplex virus type 2 from Chiang Mai, Thailand and Okinawa, Japan.
Genomic variations of herpes simplex viruses type 2 (HSV 2) isolated from Chiang Mai, Thailand and Okinawa, southernmost part of Japan were studied. The genomic polymorphism of 40 HSV 2 Chiang Mai strains and 10 HSV 2 Okinawan strains was analyzed by means of the variations of cleavage sites and electrophoretic mobilities of DNA fragments after digestion with 4 restriction endonucleases (RE (BamH I, Kpn I, EcoR I, and Bgl II)). Using the main 6 variable RE cleavage sites, HSV 2 strains were classified into 10 major groups. The strains categorized into group 1 and 9 were predominant in Chiang Mai. Groups 1, 3, 4, 5, 6 and 7 were found only in Chiang Mai, but contained only a few strains each. Groups 2 and 10 were found only in Okinawa, whereas groups 8 and 9 were found in both Chiang Mai and Okinawa.
Sirirungsi
W
W
Department of Pathology, Ryukuyu University School of Medicine, Okinawa, Japan.
Sunagawa
K
K
Iwamasa
T
T
D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D006558:Herpes Genitalis / Q000821:virology; D018258:Herpesvirus 2, Human / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007564:Japan; D011110:Polymorphism, Genetic; D015183:Restriction Mapping; D013045:Species Specificity; D013785:Thailand
D004279:DNA, Viral; D004262:DNA Restriction Enzymes