Effects of cycloheximide on chromatin-bound and -unbound PCNA in HeLa cells.
We examined the effects of cycloheximide on the amount of chromatin-bound and -unbound PCNA subpopulations in HeLa cells. The chromatin-bound PCNA content decreased rapidly in the presence of cycloheximide, whereas the reduction of the total amount of PCNA was minimal even 24 h after the treatment with cycloheximide. From these data, the half-life of PCNA was estimated to be 31.0 h for chromatin-bound PCNA and 276.8 h for the total.
Sasaki
K
K
Department of Pathology, Iwate Medical University, Morioka, Japan.
Kurose
A
A
Uesugi
N
N
Kato
A
A
D002843:Chromatin / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology*; D006207:Half-Life; D006367:HeLa Cells / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D018809:Proliferating Cell Nuclear Antigen / Q000187:drug effects* / Q000378:metabolism
D002843:Chromatin; D018809:Proliferating Cell Nuclear Antigen; D003513:Cycloheximide