Enhancement of rotavirus infectivity by saturated fatty acids.
The effect of different saturated fatty acids from 10 to 16 carbon atom chains and some derivatives on the infectivity of SA-11 rotavirus was examined. Both fatty acids and derivatives induced an increase of rotavirus infected LLC-MK2 cells when present during viral absorption to host cells. Capric acid and palmitic acid were the most effective with a dose-dependent relationship. These last lipids, in the same experimental conditions, failed to restore the susceptibility to infection of LLC-MK2 cells made resistant by neuraminidase treatment or to allow cell infection by non-infectious single-shelled viral particles. Results obtained suggest that the enhancing effect on viral infectivity by saturated fatty acids requires previous binding of rotaviral outer capsid proteins to sialic acid containing cell receptors.
Superti
F
F
Department of Ultrastructures, Istituto Superiore di Sanita, Rome, Italy.
Marziano
M L
ML
Donelli
G
G
Marchetti
M
M
Seganti
L
L
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005227:Fatty Acids / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000166:cytology / Q000821:virology*; D008251:Macaca; D009439:Neuraminidase / Q000494:pharmacology; D012401:Rotavirus / Q000187:drug effects* / Q000502:physiology; D014771:Virion / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
D005227:Fatty Acids; D009439:Neuraminidase