title: Spectrofluorometric assay for rapid detection of total and fecal coliforms from surface water.
abstract: With a spectrofluorometer, the length of the incubation time required in the fluorogenic assay was reduced to 12 h. The threshold emissions for reading the fluorogenic reaction by the spectrofluorometer were 5 and 10 U for lauryl tryptose broth media containing 4-methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide, respectively. These two kinds of threshold units were equivalent to known concentrations of free 4-methylumbelliferone of 2.5 and 6 microM, respectively, in lauryl tryptose broth media.
authors.affiliation: Department of Microbiology, College of Sciences, Taejon University, Korea.
authors.forename: S J
authors.initials: SJ
authors.lastname: Park
authors.forename: E J
authors.initials: EJ
authors.lastname: Lee
authors.forename: D H
authors.initials: DH
authors.lastname: Lee
authors.forename: S H
authors.initials: SH
authors.lastname: Lee
authors.forename: S J
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D001431:Bacteriological Techniques; D003470:Culture Media; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D005456:Fluorescent Dyes; D005618:Fresh Water; D005697:Galactosides; D006923:Hymecromone / Q000031:analogs & derivatives; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D014871:Water Microbiology
chemical_list: D003470:Culture Media; D005456:Fluorescent Dyes; D005697:Galactosides; D006923:Hymecromone; C026845:4-methylumbelliferyl-galactopyranoside; C034888:4-methylumbelliferyl glucuronide