Construction of an Actinobacillus pleuropneumoniae-Escherichia coli cosmid cloning vector [Gene 160 (1995) 81-86].
We constructed a cosmid vector, pSW206, which should be useful for the construction of genomic libraries of Actinobacillus pleuropneumoniae (Apl) DNA. pSW206 is based on the broad-host-range plasmid RK2 and can be introduced into Apl by conjugation.
West
S E
SE
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706, USA.
Romero
M J
MJ
D016977:Actinobacillus pleuropneumoniae / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D003227:Conjugation, Genetic; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D015698:Genomic Library; D010641:Phenotype; D010957:Plasmids; D015183:Restriction Mapping
D004269:DNA, Bacterial