Differential localization of heme oxygenase and NADPH-diaphorase in spinal cord neurons.
Western blot analysis using several antibodies showed that rat spinal cord contained abundant immunostainable heme oxygenase-2 (HO-2) and barely detectable levels of heme oxygenase-1 (HO-1). Anti-HO-2 antibody stained large anterior horn motoneurones and numerous smaller neurons throughout spinal cord gray matter including the dorsal root entry zone. HO-2+ astrocytes were not evident in gray matter although their presence cannot be ruled out. The distribution of HO-2+ neurons was compared with the distribution of cells containing NADPH-diaphorase (NADPH-d) activity, a marker for nitric oxide synthase. NADPH-d activity was restricted to far fewer neurons, many of which were close to the central canal and dorsal root entry zone.
Dwyer
B E
BE
Molecular Neurobiology Laboratory (IIIN-1), Department of Veterans Affairs Medical Center, Sepulveda, CA 91343, USA.
Nishimura
R N
RN
Lu
S Y
SY
D000818:Animals; D015153:Blotting, Western; D006360:Heat-Shock Proteins / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D007150:Immunohistochemistry; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology*; D051381:Rats; D013116:Spinal Cord / Q000166:cytology* / Q000201:enzymology*; D016707:Tissue Fixation
D006360:Heat-Shock Proteins; D006419:Heme Oxygenase (Decyclizing); D009252:NADPH Dehydrogenase