Isolation and characterization of c-fos recombinant baculovirus.
The use of the baculovirus system to produce recombinant proteins is based on the high level of protein production and the possibility to obtain, in Spodoptera frugiperda insect cells, recombinant proteins with the post-translational modifications found in the native proteins. Here we describe the isolation and characterization of a recombinant baculovirus containing the mouse c-fos gene. The c-fos cDNA was subcloned into the pVL1392 baculovirus transfer vector. The recombinant plasmid (pVL1392.fos) was introduced into Sf9 insect cells by co-transfection with viral wild-type DNA. Upon selection and characterization of a viral recombinant clone, Sf9 cells were infected with this virus stock and the cFos protein expression was detected by immunological methods using an anti-cFos polyclonal antiserum.
Corvello
C M
CM
Instituto de Química, Universidade de São Paulo, Brasil.
Bravo
R
R
Armelin
M C
MC
D000818:Animals; D016367:Baculoviridae / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000032:analysis; D051379:Mice; D016755:Proto-Oncogene Proteins c-jun / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D018411:Spodoptera; D014162:Transfection
D016755:Proto-Oncogene Proteins c-jun; D004262:DNA Restriction Enzymes