Ubiquitination of the rat uterine estrogen receptor: dependence on estradiol.
Using polyclonal antibodies against estrogen receptor and ubiquitin, the ubiquitination of the estrogen receptor has been demonstrated in both in vitro and in vivo conditions. The ubiquitination of the estrogen receptor is estradiol specific and is enhanced by estradiol. Estrogen withdrawal is associated with decreased ubiquitination of the estrogen receptor.
Nirmala
P B
PB
Department of Biochemistry, School of Life Sciences, University of Hyderabad, India.
Thampan
R V
RV
D000818:Animals; D000906:Antibodies; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D015151:Immunoblotting; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism; D010052:Ovariectomy; D051381:Rats; D017208:Rats, Wistar; D011960:Receptors, Estrogen / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D014452:Ubiquitins / Q000378:metabolism*; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D000906:Antibodies; D011960:Receptors, Estrogen; D014452:Ubiquitins; D004958:Estradiol