title: Rapid high-performance liquid chromatographic method for the quantitation of polyamines as their dansyl derivatives: application to plant and animal tissues.
abstract: A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.
authors.affiliation: Laboratori de Fisiologia Vegetal, Facultat de Farmàcia, Universitat de Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Marcé
authors.forename: D S
authors.initials: DS
authors.lastname: Brown
authors.forename: T
authors.initials: T
authors.lastname: Capell
authors.forename: X
authors.initials: X
authors.lastname: Figueras
authors.forename: A F
authors.initials: AF
authors.lastname: Tiburcio
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003619:Dansyl Compounds / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D011073:Polyamines / Q000032:analysis*; D051381:Rats; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D003619:Dansyl Compounds; D011073:Polyamines