Structure determination of galacto-oligosaccharides by pyridylamination and NMR spectroscopy.
Galacto-oligosaccharides formed from lactose by the action of some beta-galactosidases were subjected to gel chromatography on Bio-Gel P-2, and the resulting oligosaccharide fractions were converted into pyridylamino (PA) derivatives. Each PA-oligosaccharide fraction, which consisted of several isomers in a given size-class, was then  subjected to HPLC on an ODS column. Twenty-one individual galacto-oligosaccharide components were isolated in this way. The structures of most of these compounds, namely six disaccharides, five trisaccharides, two tetrasaccharides, and a pentasaccharide, were determined by 13C-NMR spectroscopy. The results obtained will be useful for the study of the activity of various galacto-oligosaccharides on the growth of Bifidobacterium species.
Kimura
K
K
YAKULT Central Institute for Microbiological Research, Tokyo, Japan.
Matsumoto
K
K
Ishihara
C
C
Harada
K
K
Miyagi
A
A
D000631:Aminopyridines / Q000378:metabolism; D002240:Carbohydrate Sequence; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D005697:Galactosides / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D001616:beta-Galactosidase / Q000378:metabolism
D000631:Aminopyridines; D005697:Galactosides; D009844:Oligosaccharides; D001616:beta-Galactosidase