Inhibition of protein phosphorylation modulates expression of the Jak family protein tyrosine kinases.
Treatment of murine Friend cells with a dose of the protein kinase inhibitor staurosporine, which is able to block the response of the cells to interferons, appears to inhibit phosphorylation of Jak proteins and, interestingly, to specifically reduce tyk2 and Jak1 expression and to increase Jak2 both in the presence and in the absence of interferons. Therefore, a potential role for phosphorylation events in the regulation of expression of the Jak family members is suggested.
Fiorucci
G
G
Laboratory of Virology, Istituto Superiore di Sanità, Rome, Italy.
Percario
Z A
ZA
Marcolin
C
C
Coccia
E M
EM
Affabris
E
E
Romeo
G
G
D000470:Alkaloids / Q000494:pharmacology*; D000818:Animals; D005622:Friend murine leukemia virus / Q000187:drug effects / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects; D016898:Interferon-alpha / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D053613:Janus Kinase 1; D053614:Janus Kinase 2; D007942:Leukemia, Experimental; D051379:Mice; D010766:Phosphorylation; D014176:Protein Biosynthesis; D047428:Protein Kinase Inhibitors; D011505:Protein-Tyrosine Kinases / Q000096:biosynthesis*; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D019311:Staurosporine; D053634:TYK2 Kinase; D014407:Tumor Cells, Cultured
D000470:Alkaloids; D016898:Interferon-alpha; D047428:Protein Kinase Inhibitors; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D007371:Interferon-gamma; D011505:Protein-Tyrosine Kinases; C507905:Jak1 protein, mouse; C507926:Jak2 protein, mouse; D053613:Janus Kinase 1; D053614:Janus Kinase 2; D053634:TYK2 Kinase; C499356:Tyk2 protein, mouse; D019311:Staurosporine