DLD-1 and HCT-15 cell lines derived separately from colorectal carcinomas have totally different chromosome changes but the same genetic origin.
Aneuploid DLD-1 and HCT-15 cell lines independently derived from a colon carcinoma by two researchers lacked a common marker chromosome or a concurrent numerical change. To further look into the genetic identity of these two cell lines, we used chromosome painting with fluorescence in situ hybridization (FISH) and confirmed the G-band identification on all chromosome changes. We have also found comparable results between these two cell lines on C-/Q- polymorphic bands of several chromosomes, and electrophoretic patterns of restriction fragment-length polymorphisms (RFLPs) from eight Y-chromosome-specific DNA probes with 13 restriction enzymes. These results strongly suggest that DLD-1 and HCT-15 are identical genetically. Clearly, cell lines bearing totally unrelated changes in karotypes can be generated from the same cancer specimen.
Chen
T R
TR
American Type Culture Collection, Rockville, MD 20852, USA.
Dorotinsky
C S
CS
McGuire
L J
LJ
Macy
M L
ML
Hay
R J
RJ
D015139:Blotting, Southern; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D002877:Chromosomes, Human / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D006801:Humans; D007621:Karyotyping; D011110:Polymorphism, Genetic; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm