Spectrofluorometric assay for rapid detection of total and fecal coliforms from surface water.
With a spectrofluorometer, the length of the incubation time required in the fluorogenic assay was reduced to 12 h. The threshold emissions for reading the fluorogenic reaction by the spectrofluorometer were 5 and 10 U for lauryl tryptose broth media containing 4-methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide, respectively. These two kinds of threshold units were equivalent to known concentrations of free 4-methylumbelliferone of 2.5 and 6 microM, respectively, in lauryl tryptose broth media.
Park
S J
SJ
Department of Microbiology, College of Sciences, Taejon University, Korea.
Lee
E J
EJ
Lee
D H
DH
Lee
S H
SH
Kim
S J
SJ
D001431:Bacteriological Techniques; D003470:Culture Media; D004755:Enterobacteriaceae / Q000302:isolation & purification*; D005456:Fluorescent Dyes; D005618:Fresh Water; D005697:Galactosides; D006923:Hymecromone / Q000031:analogs & derivatives; D013050:Spectrometry, Fluorescence; D013997:Time Factors; D014871:Water Microbiology
D003470:Culture Media; D005456:Fluorescent Dyes; D005697:Galactosides; D006923:Hymecromone; C026845:4-methylumbelliferyl-galactopyranoside; C034888:4-methylumbelliferyl glucuronide