Optic nerve hypoplasia in the fetal alcohol syndrome: a mouse model.
Optic nerve hypoplasia is commonly observed in children affected by the fetal alcohol syndrome, and is believed to contribute to their poor visual acuity. We have used a 'binge' model of alcohol abuse in an attempt to recreate this hypoplasia in a mouse model. Pregnant female (C57BL/6 x CBA)F1 mice were injected intraperitoneally with a single dose of a 25% solution of ethanol (v:w), either on d 11 or d 12 of gestation. Optic nerves were prepared for transmission electron microscopy from offspring at 3, 6, 9 and 15 wk of age (n = 64). A systematic random sampling technique was used to analyse both the cross-sectional areas of the optic nerves from semithin sections, and the numbers and cross-sectional areas of myelinated axons from thin sections. We found no significant differences either in the cross-sectional area or in the number of axons in the optic nerves between 3 and 9 wk from control and alcohol-treated groups. From 9 to 15 wk, alcohol-treated groups showed a loss of approximately 25% of myelinated axons (65,931 +/- 2806-49,186 +/- 3194: mean number of axons +/- S.E.M., respectively). Over the same period the number of axons in control groups was relatively stable (62,087 +/- 2043-64,703 +/- 3607). This resulted in an optic nerve with statistically significantly fewer myelinated axons at 15 wk in the alcohol-treated group, and was reflected in a trend towards a smaller cross-sectional area of the optic nerve in alcohol-treated groups.(ABSTRACT TRUNCATED AT 250 WORDS)
Parson
S H
SH
Department of Anatomy, University of Edinburgh, UK.
Dhillon
B
B
Findlater
G S
GS
Kaufman
M H
MH
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000648:ultrastructure; D004195:Disease Models, Animal; D000431:Ethanol / Q000008:administration & dosage; D063647:Fetal Alcohol Spectrum Disorders / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000187:drug effects / Q000648:ultrastructure; D009900:Optic Nerve / Q000002:abnormalities* / Q000187:drug effects / Q000648:ultrastructure
D000431:Ethanol