title: Experimental study to show that growth hormone treatment before trauma increases glutamine uptake in the intestinal tract.
abstract: This study examined whether growth hormone treatment deprived the intestinal tract of glutamine after trauma. Piglets were treated with growth hormone 24 units daily 3 days before and at the start of the trauma (GH-3, n = 8) or at the start of the trauma only (GH-1, n = 8). Eight piglets acted as non-treated controls. The trauma consisted of a standardized abdominal surgical procedure. Primed constant infusions of U-14C-glutamine were given. Intestinal, hepatic, renal and hindleg glutamine fluxes were measured. Growth hormone treatment increased mean(s.e.m.) net intestinal glutamine uptake: GH-3, 39.7(9.4) and 48.7(12.7) mumol/min; GH-1, 33.2(5.5) and 25.7(12.3) mumol/min; controls, 19.5(10.3) and 2.0(15.3) mumol/min at 1 h and 5 h after trauma, respectively, (P = 0.02). The treatment increased glutamine oxidation (P = 0.025), and decreased hindleg glutamine net (P = 0.0052) and absolute release (P = 0.0063), glutamine rate of appearance (P = 0.01), and percentage of glucose coming from glutamine (P = 0.05). Growth hormone treatment before trauma increased intestinal glutamine uptake.
authors.affiliation: Department of Surgery, Tromsø University Hospital, Norway.
authors.forename: M
authors.initials: M
authors.lastname: Mjaaland
authors.forename: K
authors.initials: K
authors.lastname: Unneberg
authors.forename: T G
authors.initials: TG
authors.lastname: Jenssen
authors.forename: A
authors.initials: A
authors.lastname: Revhaug
mesh_terms: D000005:Abdomen / Q000601:surgery*; D000818:Animals; D005947:Glucose / Q000378:metabolism; D005973:Glutamine / Q000493:pharmacokinetics*; D013006:Growth Hormone / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000378:metabolism*; D011897:Random Allocation; D013552:Swine
chemical_list: D005973:Glutamine; D013006:Growth Hormone; D005947:Glucose