Induction of the antioxidant stress proteins heme oxygenase-1 and MSP23 by stress agents and oxidised LDL in cultured vascular smooth muscle cells.
Enhanced expression of the antioxidant stress proteins heme oxygenase-1 (HO-1) and macrophage stress protein (MSP23) by oxidative stress agents and oxidatively modified low density lipoproteins (LDL) was investigated in cultured porcine aortic smooth muscle cells. Treatment of smooth muscle cells with glucose oxidase, CdCl2 or diethylmaleate resulted in a time-dependent (6-48 h) induction of HO-1 and MSP23 expression. Exposure of cells to 100 micrograms protein/ml highly oxidised LDL increased the expression of HO-1 and MSP23 within 24 h, and the induction was dependent on the degree of LDL oxidation. The induction of HO-1 and MSP23 may thus play an important cytoprotective role against oxidative stress in atherogenesis.
Siow
R C
RC
Vascular Biology Research Centre, King's College, London, UK.
Ishii
T
T
Sato
H
H
Taketani
S
S
Leake
D S
DS
Sweiry
J H
JH
Pearson
J D
JD
Bannai
S
S
Mann
G E
GE
D000818:Animals; D001011:Aorta; D002104:Cadmium / Q000494:pharmacology; D019256:Cadmium Chloride; D002478:Cells, Cultured; D002712:Chlorides / Q000494:pharmacology; D004790:Enzyme Induction; D005949:Glucose Oxidase / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis*; D008077:Lipoproteins, LDL / Q000378:metabolism / Q000494:pharmacology; D008298:Maleates / Q000494:pharmacology; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010544:Peroxidases; D013552:Swine
D002712:Chlorides; D006360:Heat-Shock Proteins; D008077:Lipoproteins, LDL; D008298:Maleates; D002104:Cadmium; D005949:Glucose Oxidase; D010544:Peroxidases; D006419:Heme Oxygenase (Decyclizing); C014476:diethyl maleate; D019256:Cadmium Chloride