title: Suppression of immune parameters in animal models of morphine dependence.
abstract: Implantation of pellets containing 75 mg of morphine induced short term (4 day) morphine dependence and markedly reduced total number of spleen cells of BALB/c mice, without affecting total body or liver weight. Polyclonal responses induced by anti-CD3 antibodies, Concanavalin A or Escherichia coli lipopolysaccharide in the remaining spleen cells of morphine-treated mice were also inhibited. Cytofluorimetric analysis indicated that the proportion of major functional lymphocyte populations (Ig+, CD3+, CD4+ and CD8+ lymphocytes) were not significantly changed in the spleen from morphine-dependent mice. Furthermore, expression levels of surface Ig, CD3, CD4, and CD8, were similar in spleen cells from control or morphine-treated mice. So, morphine dependence in BALB/c mice under these controlled conditions results in a specific defect in lymphoid cell number and function, with no incidence on body weight or particular lymphocyte subsets.
authors.affiliation: Departamento de Nefrología, Hospital Clínico de San Carlos, Madrid, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Portolés
authors.forename: G
authors.initials: G
authors.lastname: Ojeda
authors.forename: M
authors.initials: M
authors.lastname: Ronda
authors.forename: J C
authors.initials: JC
authors.lastname: Leza
authors.forename: J M
authors.initials: JM
authors.lastname: Rojo
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003864:Depression, Chemical; D004343:Drug Implants; D007153:Immunologic Deficiency Syndromes / Q000097:blood / Q000139:chemically induced* / Q000276:immunology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008099:Liver / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects; D018655:Lymphocyte Count / Q000187:drug effects*; D016131:Lymphocyte Subsets / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009020:Morphine / Q000494:pharmacology* / Q000633:toxicity; D009021:Morphine Dependence / Q000276:immunology*; D009929:Organ Size / Q000187:drug effects; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000473:pathology
chemical_list: D004343:Drug Implants; D007166:Immunosuppressive Agents; D011947:Receptors, Antigen, B-Cell; D009020:Morphine