Molecular mimicry and ankylosing spondylitis: possible role of a novel sequence in pullulanase of Klebsiella pneumoniae.
Molecular mimicry has been shown between two sequences of Klebsiella pneumoniae pulD secretion protein (DRDE) with HLA-B27 (DRED) and pulA (pullulanase) enzyme (Gly-X-Pro) with types I, III and IV collagen respectively. IgG antibody levels in AS patients were elevated against 16mer synthetic peptides of HLA-B27 and pulD by enzyme immunosorbent assay (ELISA) compared to controls (P < 0.001). ELISA assays against K. pneumoniae grown in the absence and presence of pullulan demonstrated significant levels of IgA antibody in AS patients compared to controls (P < 0.001). Increased IgA and IgG antibody levels to pulA and types I and IV collagen were observed in AS patients compared to controls (P < 0.001). These observations could be relevant in the sequence of molecular events in AS.
Fielder
M
M
Division of Life Sciences, King's College, London, UK.
Pirt
S J
SJ
Tarpey
I
I
Wilson
C
C
Cunningham
P
P
Ettelaie
C
C
Binder
A
A
Bansal
S
S
Ebringer
A
A
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000097:blood*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000276:immunology; D003094:Collagen / Q000235:genetics; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005936:Glucans; D006026:Glycoside Hydrolases / Q000235:genetics*; D015796:HLA-B27 Antigen / Q000235:genetics / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D013167:Spondylitis, Ankylosing / Q000235:genetics / Q000276:immunology* / Q000382:microbiology
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D005936:Glucans; D015796:HLA-B27 Antigen; D007074:Immunoglobulin G; D010455:Peptides; C061377:PulD protein, Klebsiella pneumoniae; C009109:pullulan; D003094:Collagen; D006026:Glycoside Hydrolases; C100181:pullulanase