There is no difference in spontaneous and 17 beta-estradiol-induced interleukin-1 beta release by peripheral blood mononuclear cells from nonosteoporotic women with different rates of early postmenopausal bone loss.
Interleukin-1 (IL-1) is a potent stimulator of bone resorption, and a causal role for IL-1 has been suggested in postmenopausal bone loss. We have examined IL-1 beta release in vitro by peripheral blood mononuclear cells (PBMC) isolated from nonosteoporotic women 9-15 yr after menopause. These women had presented 6 yr previously with significant differences in the rate of early postmenopausal bone loss. Ten women with low rates of bone loss (median 2.0% per year) and 10 women with high rates of bone loss (median 4.9% per year) were included in the study. The women with a high rate of bone loss had a significantly lower bone mass of the lumbar vertebrae compared with that of the other group, but there were no differences in biochemical markers of bone metabolism between the groups (pyridinoline/creatinine ratio in urine and collagen 1 c-terminal telopeptide and bone gla protein in serum). Moreover, there was no difference in spontaneous IL-1 beta release by PBMCs between the two groups and no correlation between IL-1 beta release and present bone turnover, as judged by biochemical markers. Treatment of PBMCs with 10 nmol/L 17 beta-estradiol in vitro significantly stimulated IL-1 beta production in both groups. We conclude that IL-1 beta production by PBMCs in vitro does not correlate with the rate of early postmenopausal bone loss.
Høgåsen
A K
AK
Department of Pediatric Research, National Hospital, Oslo, Norway.
Nordsletten
L
L
Aasen
A O
AO
Falch
J A
JA
D000596:Amino Acids / Q000097:blood; D000704:Analysis of Variance; D015415:Biomarkers / Q000097:blood; D001862:Bone Resorption; D002478:Cells, Cultured; D003404:Creatinine / Q000097:blood; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006801:Humans; D007375:Interleukin-1 / Q000097:blood / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology*; D008875:Middle Aged; D015675:Osteocalcin / Q000097:blood; D015663:Osteoporosis, Postmenopausal / Q000097:blood / Q000276:immunology*; D017698:Postmenopause / Q000097:blood / Q000276:immunology*; D013997:Time Factors
D000596:Amino Acids; D015415:Biomarkers; D007375:Interleukin-1; D015675:Osteocalcin; D004958:Estradiol; C015484:pyridinoline; D003404:Creatinine