Characterization of recombinant tetanus toxin derivatives suitable for vaccine development.
Recombinant derivatives of tetanus toxin (TeTx) were isolated and used to immunize mice. Recombinant TeTx light chain, a derivative of fragment C that had lost the ability to bind neurons, and a recombinant TeTx holotoxoid that could protect mice against TeTx challenge were identified.
Figueiredo
D
D
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, United Kingdom.
Turcotte
C
C
Frankel
G
G
Li
Y
Y
Dolly
O
O
Wilkin
G
G
Marriott
D
D
Fairweather
N
N
Dougan
G
G
D000818:Animals; D001426:Bacterial Proteins / Q000096:biosynthesis; D001428:Bacterial Vaccines / Q000276:immunology*; D005727:Ganglia, Spinal / Q000378:metabolism; D051379:Mice; D011485:Protein Binding; D051381:Rats; D011993:Recombinant Fusion Proteins; D013329:Structure-Activity Relationship; D013744:Tetanus Toxin / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D014614:Vaccines, Synthetic / Q000276:immunology*
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D011993:Recombinant Fusion Proteins; D013744:Tetanus Toxin; D014614:Vaccines, Synthetic