title: Replication of measles virus in human monocytes and T cells.
abstract: Replication of measles virus (MV) in populations of peripheral blood mononuclear cells enriched for T cells and monocytes was studied using a temperature-sensitive mutant, MV ts38, and the parent counterpart, MV Lec. Stimulation of the cells was required for a full cycle of virus replication in both cell types. More infectious virus was released after stimulation from MV-infected populations enriched for T cells, T cell-enriched than from monocyte-enriched populations. However, similar amounts of viral mRNA, genomic RNA, and viral proteins of the expected size were found in both cell populations. The results indicate that MV-specific macromolecular synthesis is similar in both T cells and monocytes, but the assembly and (or) release of infectious virus is greatly reduced in monocytes as compared with T cells.
authors.affiliation: Department of Virology, University of Turku, Finland.
authors.forename: S
authors.initials: S
authors.lastname: Vydelingum
authors.forename: K
authors.initials: K
authors.lastname: Suryanarayana
authors.forename: R G
authors.initials: RG
authors.lastname: Marusyk
authors.forename: A A
authors.initials: AA
authors.lastname: Salmi
mesh_terms: D002455:Cell Division; D006801:Humans; D008459:Measles virus / Q000235:genetics / Q000502:physiology*; D009000:Monocytes / Q000166:cytology / Q000821:virology*; D009154:Mutation; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000166:cytology / Q000821:virology*; D013696:Temperature; D015678:Viral Structural Proteins / Q000096:biosynthesis; D014779:Virus Replication
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D015678:Viral Structural Proteins