title: Effects of gamma radiation on the OM431 human ocular melanoma cell line.
abstract: In order to determine the dose responsiveness to radiation of ocular melanoma, we conducted an in vitro dose-response study on a monolayer cell culture using a clonogenic assay. The effects on cell survival were determined relative to unirradiated controls. A human epithelioid ocular melanoma cell line, OM431, was maintained in tissue culture and serial dilutions of viable cells were plated in flasks, allowed to settle and attach for 48 h, and subsequently irradiated with 1-10 Gy in single fractions. After 2 weeks, the number of reproducing clones (forming colonies with greater than 32 cells or five generations) were counted. The surviving fractions of cells were plotted on a cell survival curve using the linear quadratic model. The survival curve showed a large initial shoulder followed by an exponential decline in growth. Our data suggest that the OM431 ocular melanoma cell line responds to irradiation in a manner similar to other melanoma cell lines and is relatively radioresistant especially at lower doses.
authors.affiliation: Department of Ophthalmology, University of California, School of Medicine, Los Angeles 90024-7000, USA.
authors.forename: S
authors.initials: S
authors.lastname: Logani
authors.forename: A S
authors.initials: AS
authors.lastname: Cho
authors.forename: L D
authors.initials: LD
authors.lastname: Su
authors.forename: H R
authors.initials: HR
authors.lastname: Withers
authors.forename: W H
authors.initials: WH
authors.lastname: McBride
authors.forename: M O
authors.initials: MO
authors.lastname: Hall
authors.forename: D A
authors.initials: DA
authors.lastname: Lee
authors.forename: J K
authors.initials: JK
authors.lastname: Milani
authors.forename: B R
authors.initials: BR
authors.lastname: Straatsma
mesh_terms: D002470:Cell Survival / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005134:Eye Neoplasms / Q000532:radiotherapy*; D005720:Gamma Rays; D006801:Humans; D008545:Melanoma / Q000532:radiotherapy*; D014407:Tumor Cells, Cultured; D014410:Tumor Stem Cell Assay