title: 1H NMR investigation of the interaction of berberine and sanguinarine with DNA.
abstract: Interaction of the alkaloids, berberine and sanguinarine with calf thymus DNA has been studied by 1H NMR. All proton resonances of the two compounds have been assigned using 2D-COSY, NOESY and ROESY spectra. Berberine has been found to partially intercalate into DNA, while sanguinarine shows normal intercalation and also binds more firmly to DNA. The NMR experiments indicate that sanguinarine is more potent than berberine in its activity.
authors.affiliation: Chemical Physics Group, Tata Institute of Fundamental Research, Colaba, Bombay.
authors.forename: A
authors.initials: A
authors.lastname: Saran
authors.forename: S
authors.initials: S
authors.lastname: Srivastava
authors.forename: E
authors.initials: E
authors.lastname: Coutinho
authors.forename: M
authors.initials: M
authors.lastname: Maiti
mesh_terms: D000470:Alkaloids / Q000378:metabolism*; D000818:Animals; D053119:Benzophenanthridines; D001599:Berberine / Q000378:metabolism*; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D007364:Intercalating Agents / Q000378:metabolism*; D007546:Isoquinolines; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons
chemical_list: D000470:Alkaloids; D053119:Benzophenanthridines; D007364:Intercalating Agents; D007546:Isoquinolines; D011522:Protons; D001599:Berberine; D004247:DNA; C005705:sanguinarine