Cloning of a new FRTL5-derived cell line stably expressing active human thyroid peroxidase.
A full-length cDNA clone for human thyroid peroxidase inserted into the mammalian cell expression vector pECE was stably transfected into the rat thyroid cell line FRTL5. Clones expressed immunologically and enzymatically assessed human thyroid peroxidase protein. Methimazole (25 microM) inhibited the thyroid peroxidase activity dose-dependently and this effect was completely antagonised by 100 microM NaI. Ethylenethiourea, metabolite of dithiocarbamate pesticides, inhibited the enzyme at 50 microM. Thus, we have obtained thyroidal cells stably expressing enzymatically active human thyroid peroxidase which can be pharmacologically modulated and studied.
Marinovich
M
M
Lab. of Toxicology, University of Milan, Italy.
Guizzetti
M
M
Zanelli
T
T
Corsini
E
E
Galli
C L
CL
D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D002460:Cell Line; D003001:Cloning, Molecular; D006224:Cricetinae; D005031:Ethylenethiourea / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007453:Iodide Peroxidase / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D007700:Kinetics; D008713:Methimazole / Q000494:pharmacology; D051381:Rats; D013961:Thyroid Gland / Q000201:enzymology*; D014162:Transfection
D005031:Ethylenethiourea; D008713:Methimazole; D006861:Hydrogen Peroxide; D007453:Iodide Peroxidase