Production of chitinase by Pseudomonas aeruginosa K-187 using shrimp and crab shell powder as a carbon source.
Shrimp and crab shell powder prepared by treating shrimp and crab processing waste with boiling and crashing was used as a substrate for isolating alkali-tolerant chitinolytic microorganisms. Strain K-187 appeared to be the chitinase-producing strain with the most potential. The organism was identified as a strain of Pseudomonas aeruginosa. Maximum chitinase activity was obtained when the strain was grown aerobically in a medium consisting of 3.0% shrimp and crab shell powder, 0.1% CMC, 0.1% (NH4)2SO4, 0.1% K2HPO4, 0.1% MgSO4.D7H2O and 0.1% ZnSO4 (pH 9), at 45 degrees C after 3 days. The optimum pH and temperature of the enzyme reaction were 7 and 40 degrees C, respectively. The chitinase was stable at pH from 5 to 10 and was stable under 60 degrees C.
Wang
S L
SL
Department of Food Engineering, Da-Yeh Institute of Technology, Chang-Hwa, Taiwan, Republic of China.
Chang
W T
WT
Lu
M C
MC
D000818:Animals; D003386:Brachyura; D002244:Carbon / Q000378:metabolism*; D002686:Chitin / Q000494:pharmacology; D002688:Chitinases / Q000096:biosynthesis* / Q000378:metabolism; D003470:Culture Media; D033364:Decapoda; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009584:Nitrogen / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D013696:Temperature
D003470:Culture Media; D002686:Chitin; D002244:Carbon; D002688:Chitinases; D009584:Nitrogen