[Dysfunction of E-cadherin due to mutation of beta-catenin in a scirrhous gastric cancer cell line].
E-cadherin is a calcium dependent cell-cell adhesion molecule. In cancer tissue, detachment of the adhesion system is indispensable for invasion and metastasis of cancer cells. We have investigated mechanism of the dysfunction of E-cadherin-dependent cell-cell adhesion system in gastric carcinoma cells. Although the high expression of E-cadherin in a scirrhous gastric cancer cell line HSC-39, the function of E-cadherin was completely abolished. Western blotting of beta-catenin in HSC-39 cells demonstrated that a truncated beta-catenin was detected. The truncation was due to partial deletion of beta-catenin DNA. It was concluded that in HSC-39 loss of E-cadherin dependent cell-cell adhesion was due to mutation of beta-catenin gene.
Kawanishi
J
J
Fourth Department of Internal Medicine, Sapporo Medical University School of Medicine.
Kato
J
J
Sasaki
K
K
Fujii
S
S
Watanabe
N
N
Niitsu
Y
Y
D002293:Adenocarcinoma, Scirrhous / Q000235:genetics / Q000473:pathology*; D015820:Cadherins / Q000235:genetics*; D003598:Cytoskeletal Proteins / Q000235:genetics*; D017353:Gene Deletion; D006801:Humans; D009154:Mutation; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D015534:Trans-Activators; D014407:Tumor Cells, Cultured; D051176:beta Catenin
C495270:CTNNB1 protein, human; D015820:Cadherins; D003598:Cytoskeletal Proteins; D015534:Trans-Activators; D051176:beta Catenin