Photocleavable biotin derivatives: a versatile approach for the isolation of biomolecules.
While the strong biotin-avidin interaction has been widely used for the detection of biomolecules, its irreversibility complicates their isolation. We report the synthesis of a photocleavable biotin derivative (PCB) which eliminates many limitations of existing methods. This reagent contains a biotin moiety linked through a spacer arm to a photocleavable moiety, which reacts selectively with primary amino groups on any substrate. In experiments using [leucine]-enkephalin as a model substrate, we show that PCB retains its high affinity toward avidin/streptavidin and allows rapid (< 5 min) and efficient (> 99%) photorelease of the substrate in a completely unaltered form. Photocleavable biotins should be useful in numerous applications involving the isolation of proteins, nucleic acids, lipids, and cells.
Olejnik
J
J
Physics Department, Boston University, MA 02215, USA.
Sonar
S
S
Krzymañska-Olejnik
E
E
Rothschild
K J
KJ
D000595:Amino Acid Sequence; D055592:Biophysical Phenomena; D001703:Biophysics; D001710:Biotin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000528:radiation effects*; D004743:Enkephalin, Leucine / Q000737:chemistry; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010777:Photochemistry
D007202:Indicators and Reagents; D004743:Enkephalin, Leucine; D001710:Biotin