title: Characterization of verocytotoxin-producing Escherichia coli O157 isolates from patients with haemolytic uraemic syndrome in Western Europe.
abstract: Fifty verocytotoxin (VT)-producing Escherichia coli (VTEC) strains of serogroup O157 were characterized by phage typing, polymerase chain reaction (PCR) for VT genes and the E. coli attaching and effacing (eae) gene, and random amplified polymorphic DNA-PCR (RAPD-PCR) fingerprinting. The collection represented isolates obtained from patients with diarrhoea-associated haemolytic-uraemic syndrome (D+ HUS) and their family contacts, isolated in the Netherlands, Belgium and Germany between 1989 and 1993. Based on isolates from separate families (n = 27) seven different phage types were identified, types 2 (44%) and 4 (33%) were predominant. Eighty-five percent of the strains contained only VT2 gene sequences and 15% both VT1 and VT2. All strains of the dominant phage types 2 and 4 carried the VT2 gene. Strains that belonged to the minor phage types 8, 14, 32 carried both VT1 and VT2 genes, with the exception of two isolates identified as phage types 49 and 54 which contained only VT2 genes. All O157 VTEC strains possessed the chromosomally-located eae gene, which indicates its usefulness as virulence marker. RAPD-PCR fingerprinting identified four distinct banding patterns, with one profile found among 79% of the strains. Based on the combined results of all typing methods used in this study, the collection of 50 O157 VTEC strains could be divided into nine distinct groups. Strains isolated from different persons within one family could not be distinguished by any of these methods. The data suggest that O157 VTEC strains are members of one clone that has become widely distributed.
authors.affiliation: Department of Medical Microbiology, University Hospital Nijmegen, The Netherlands.
authors.forename: A E
authors.initials: AE
authors.lastname: Heuvelink
authors.forename: N C
authors.initials: NC
authors.lastname: van de Kar
authors.forename: J F
authors.initials: JF
authors.lastname: Meis
authors.forename: L A
authors.initials: LA
authors.lastname: Monnens
authors.forename: W J
authors.initials: WJ
authors.lastname: Melchers
mesh_terms: D000328:Adult; D001427:Bacterial Toxins / Q000096:biosynthesis; D001434:Bacteriophage Typing; D001483:Base Sequence; D002648:Child; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D005060:Europe; D006463:Hemolytic-Uremic Syndrome / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012703:Serotyping; D022622:Shiga Toxin 1
chemical_list: D001427:Bacterial Toxins; D022622:Shiga Toxin 1