2-Mercaptopropionylglycine modulates the expression of c-jun and c-Ha-ras in regenerating rat liver.
Changes in the expression of two proto-oncogenes, c-jun and c-Ha-ras and their modulation by MPG were studied in regenerating rat liver. A significant increase in c-jun and c-Ha-ras mRNA levels was noted after partial hepatectomy. However, the increase in c-Ha-ras mRNA was much less when compared with c-jun mRNA levels. Metallothionein (MT-I) mRNA levels showed a gradual decrease following partial hepatectomy. However, the decrease in MT-I mRNA levels was not significant when intertime comparisons were made. MPG caused a significant reduction in the levels of c-jun and c-Ha-ras mRNA in the regenerating rat liver compared to untreated controls, but had very little effect on MT-I mRNA levels. Selective reduction in the expression of proto-oncogenes by MPG suggests that MPG may affect the cell proliferation by modulating the expression of these genes.
Sharma
D
D
Department of Zoology, University of Delhi, India.
Radhika
S
S
Muralidhar
K
K
Dixit
A
A
D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects; D016758:Genes, jun / Q000187:drug effects*; D011905:Genes, ras / Q000187:drug effects*; D008115:Liver Regeneration / Q000235:genetics / Q000502:physiology*; D008668:Metallothionein / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D008625:Tiopronin / Q000494:pharmacology*; D018631:ras Proteins / Q000187:drug effects* / Q000378:metabolism
D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D008668:Metallothionein; D008625:Tiopronin; D018631:ras Proteins