Determination of the new fluoroquinolone balofloxacin and its metabolites in biological fluids by high performance liquid chromatography.
A sensitive high performance liquid chromatographic method for the determination in biological fluids of 1-cyclopropyl-6-fluoro-1,4- dihydro-8-methoxy-7-(3-methylaminopiperidin-1-yl)-4-oxoquinoline-3 -carboxylic acid dihydrate (CAS 127294-70-6, balofloxacin, Q-35), a new fluoroquinolone, as well as its metabolites, has been developed. Balofloxacin and its metabolite, N-desmethyl balofloxacin, were separated and determined by reversed-phase column with fluorescence detection. Balofloxacin glucuronide was determined as balofloxacin after alkali hydrolysis. This method exhibited good precision and accuracy. Furthermore, it is a simple method and requires only a small amount of samples, and therefore should be useful for pharmacokinetic studies in animals and humans.
Nakagawa
T
T
Drug Metabolism and Pharmacokinetics Research Laboratory, Chugai Pharmaceutical Co., Ltd., Tokyo, Japan.
Ishigai
M
M
Hiramatsu
Y
Y
Kinoshita
H
H
Ishitani
Y
Y
Ohkubo
K
K
Okazaki
A
A
D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D001646:Bile / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D024841:Fluoroquinolones; D005965:Glucuronates / Q000032:analysis; D006801:Humans; D006868:Hydrolysis; D015363:Quinolones / Q000032:analysis* / Q000097:blood / Q000652:urine; D051381:Rats; D013050:Spectrometry, Fluorescence
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D005965:Glucuronates; D015363:Quinolones; C077155:balofloxacin