Identification of Pseudomonas aeruginosa glpM, whose gene product is required for efficient alginate biosynthesis from various carbon sources.
In a mucB (algN) genetic background, insertion of an omega element approximately 200 bp downstream of glpD, encoding sn-glycerol-3-phosphate dehydrogenase from Pseudomonas aeruginosa, had an adverse effect on alginate biosynthesis from various carbon sources. The insertion inactivated glpM, a gene encoding a 12,040-M(r) hydrophobic protein containing 109 amino acids. This protein, which was expressed in a T7 RNA polymerase expression system, appears to be a cytoplasmic membrane protein.
Schweizer
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Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Alberta, Canada.
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D000464:Alginates / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D002451:Cell Compartmentation; D004926:Escherichia coli / Q000235:genetics; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis; D015183:Restriction Mapping
D000464:Alginates; D001426:Bacterial Proteins; C095151:GlpM protein, Pseudomonas aeruginosa; D008565:Membrane Proteins; D011994:Recombinant Proteins; D005993:Glycerolphosphate Dehydrogenase