[Prognostic evaluation of small cell lung carcinoma by flow cytometry. A retrospective study from bronchial brushing samples].
The authors determined the desoxyribonucleic acid (DNA) content of 45 small-cell lung cancers, by flow-cytometry (FC), from bronchial brushings to asses the prognostic meaning of the DNA index, the percentage of cells in DNA synthesis phase (% S) and the ploidy. Moreover, the value of this tumorous cells brushing was assessed for Flow-cytometric analysis. Bronchial brushing offers some advantages opposite to biopsies, but the low quantity of collected cells reduces the number of tumors analysable by FC. The output is low (39%) for determination of DNA index, and moreover low (22%) for determination of % S. In regard to the flow-cytometry analysis, the DNA content does not show any significant difference of survival where as the % S is related to survival, but it seems to be paradoxical in this study. Indeed, patients with small-cell lung cancer associated to high cells' percentage in DNA synthesis phase have the longest survival.
Jeanfaivre
T
T
Service de pneumologie, CHU, Angers, France.
Chassevent
A
A
Berruchon
J
J
Savary
L
L
Chrétien
M F
MF
Geslin
J
J
Larra
F
F
Tuchais
E
E
D001980:Bronchi / Q000473:pathology*; D001999:Bronchoscopy; D018288:Carcinoma, Small Cell / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005069:Evaluation Studies as Topic; D005260:Female; D005336:Fiber Optic Technology; D005434:Flow Cytometry; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D009367:Neoplasm Staging; D011379:Prognosis; D012189:Retrospective Studies; D015996:Survival Rate
D004273:DNA, Neoplasm
Evaluation pronostique du carcinome bronchique à petites cellules par cytométrie en flux. Etude rétrospective à partir de prélèvements par brossage bronchique.