title: Improved synthesis of 6-[p-(bromoacetamido)benzyl]-1,4,8,11-tetraazacyclotetradecane- N,N',N",N"-tetraacetic acid and development of a thin-layer assay for thiol-reactive bifunctional chelating agents.
abstract: Monoclonal antibodies labeled with radiometals such as copper-67 have applications in radioimmunodiagnosis and radioimmunotherapy. Moi et al. [(1985) Anal. Biochem. 148, 249-253] showed that 6-[p-(bromoacetamido)benzyl]-1,4,8,11-tetraazacyclotetradecane- N,N',N",N"-tetraacetic acid (BAT) is an effective reagent for linking copper to proteins, and antibodies labeled with copper radionuclides are currently undergoing clinical trials. Here we describe improvements in the original synthesis that increase the overall yield of BAT to 23%. We also describe a new assay useful to determine the activity of bifunctional chelating agents with thiol-reactive functional groups.
authors.affiliation: Department of Chemistry, University of California, Davis 95616-0935, USA.
authors.forename: J K
authors.initials: JK
authors.lastname: Moran
authors.forename: D P
authors.initials: DP
authors.lastname: Greiner
authors.forename: C F
authors.initials: CF
authors.lastname: Meares
mesh_terms: D002614:Chelating Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer / Q000379:methods*; D003432:Cross-Linking Reagents / Q000032:analysis*; D006207:Half-Life; D006571:Heterocyclic Compounds / Q000138:chemical synthesis*; D009682:Magnetic Resonance Spectroscopy; D015203:Reproducibility of Results; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D002614:Chelating Agents; D003432:Cross-Linking Reagents; D006571:Heterocyclic Compounds; D013438:Sulfhydryl Compounds; C046529:6-(4-bromoacetamidobenzyl)-1,4,8,11-tetraazacyclotetradecane-N,N',N'',N'''-tetraacetic acid