title: Increased protein secretion and adherence to HeLa cells by Shigella spp. following growth in the presence of bile salts.
abstract: Growth of Shigella spp. in the presence of the bile salt deoxycholate or chenodeoxycholate enhanced the bacterial invasion of HeLa cells. Growth in the presence of other structurally similar bile salts or detergents had little or no effect. Deoxycholate-enhanced invasion was not observed when bacteria were exposed to deoxycholate at low temperatures or when chloramphenicol was added to the growth medium, indicating that bacterial growth and protein synthesis are required. Increased invasion is associated with the presence of an intact Shigella virulence plasmid and is correlated with increased secretion of a set of proteins, including the Ipa proteins, to the outer membrane and into the growth medium. The increased invasion induced by the bile salts appears to be due to increased adherence. The enhanced adherence was specific to Shigella spp., since the enteroinvasive Escherichia coli strains tested did not exhibit the effect in response to growth in bile salts.
authors.affiliation: Department of Microbiology, University of Texas at Austin 78712, USA.
authors.forename: L M
authors.initials: LM
authors.lastname: Pope
authors.forename: K E
authors.initials: KE
authors.lastname: Reed
authors.forename: S M
authors.initials: SM
authors.lastname: Payne
mesh_terms: D018829:Adhesins, Bacterial; D001422:Bacterial Adhesion / Q000187:drug effects*; D001426:Bacterial Proteins / Q000096:biosynthesis*; D003840:Deoxycholic Acid / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D012760:Shigella / Q000187:drug effects* / Q000254:growth & development
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D003840:Deoxycholic Acid; C053476:invasin, Yersinia