title: Ultrastructural localization of alkaline phosphatase activity in human eccrine and apocrine sweat glands.
abstract: Alkaline phosphatase (ALP) is a membrane-bound enzyme that catalyzes the hydrolysis of inorganic and organic monophosphate esters at alkaline pH. Although the functions of ALP are poorly understood, it is believed to be involved in membrane transport. Because little is known about the functions and distribution of ALP in the sweat glands, we studied the localization of ALP in human sweat glands with light and electron microscopic enzyme cytochemistry. In eccrine sweat glands, ALP was restricted to the cell membranes of intercellular canaliculi. Luminal cell membranes of secretory cells that are in continuity with intercellular canaliculi did not show ALP activity. These results suggest that ALP participates in the production of primary sweat at intercellular canaliculi. In apocrine sweat glands, basal cell membranes of secretory cells and myoepithelial cell membranes that were in apposition with each other showed ALP activity, where as no activity was seen in eccrine sweat glands. These differences in the distribution of ALP in myoepithelial cells between eccrine and apocrine sweat glands might be related to the functional differences of these sweat glands. ALP histochemistry could help to diagnose and to determine the direction of differentiation in sweat gland tumors.
authors.affiliation: Department of Dermatology, Sapporo Medical University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Saga
authors.forename: Y
authors.initials: Y
authors.lastname: Morimoto
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis*; D001050:Apocrine Glands / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D004439:Eccrine Glands / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology / Q000648:ultrastructure; D006651:Histocytochemistry; D006801:Humans; D008854:Microscopy, Electron
chemical_list: D000469:Alkaline Phosphatase