title: Numerical aberrations of chromosomes 1 and 7 in renal cell carcinomas as detected by interphase cytogenetics.
abstract: Alcohol-fixed single cell suspensions of 37 renal cell carcinomas (RCCs) were assessed by both flow cytometry (FCM) and the fluorescence in situ hybridization (FISH) technique, using chromosome 1- and chromosome 7-specific centromere DNA probes. DNA diploidy or near-diploidy was observed in 30 of the 37 RCCs and only 12 of these (near-)diploid tumours were disomic for both chromosomes 1 and 7. Numerical aberrations of chromosome 1 and/or chromosome 7 were present in 18 of the 30 (near-)diploid RCCs and five of these cases showed monosomy for chromosome 1 in more than 50 per cent of the tumour cells. A double target FISH, with a centromeric and a telomeric specific probe for 1p36, excluded misinterpretation on the basis of clustering of 1q12, and suggested a complete loss of chromosome 1. All these five (near-)diploid RCCs with monosomy for chromosome 1 were eosinophilic chromophilic cell carcinomas, according to the Thoenes classification of RCC. This observation is of special interest, because it was recently concluded from cytogenetic studies that the diagnosis of chromophilic renal cell carcinoma must be considered as obsolete. Monosomy for chromosome 1 seems to be a non-random numerical aberration of (near-)diploid eosinophilic chromophilic cell carcinomas, and a gain of one or more chromosomes 1 appeared to be a common phenomenon in RCCs, especially in the DNA aneuploid tumours. As these chromosomal abnormalities were not found in the earlier classical cytogenetic studies, we conclude that in situ hybridization techniques are required in addition to chromosome banding techniques to obtain a complete characterization of the chromosome imbalances in RCCs.
authors.affiliation: Department of Pathology, University Hospital Nijmegen, The Netherlands.
authors.forename: J L
authors.initials: JL
authors.lastname: Beck
authors.forename: A H
authors.initials: AH
authors.lastname: Hopman
authors.forename: W F
authors.initials: WF
authors.lastname: Feitz
authors.forename: J
authors.initials: J
authors.lastname: Schalken
authors.forename: H E
authors.initials: HE
authors.lastname: Schaafsma
authors.forename: C A
authors.initials: CA
authors.lastname: Van de Kaa
authors.forename: F C
authors.initials: FC
authors.lastname: Ramaekers
authors.forename: A G
authors.initials: AG
authors.lastname: Hanselaar
authors.forename: P C
authors.initials: PC
authors.lastname: De Wilde
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics*; D002869:Chromosome Aberrations; D002878:Chromosomes, Human, Pair 1; D002897:Chromosomes, Human, Pair 7; D005434:Flow Cytometry; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D007680:Kidney Neoplasms / Q000235:genetics*; D009006:Monosomy