title: Soluble cytokine receptors and receptor antagonists are sequentially released after trauma.
abstract: Cytokine receptors and receptor antagonists (RAs) have been identified in trauma patients. We hypothesized that after traumatic injury, a sequential release of soluble cytokine receptors and RAs may exist that mirrors the release of the primary cytokines themselves. Twenty-two patients were included in the study: 14 males and 8 females. The mean age was 30.1 +/- 12.5 (range, 19 to 71), and the mean Injury Severity Score was 28.7 +/- 12.6 (range, 4 to 57). There were 15 survivors and 7 nonsurvivors. Samples were collected on arrival to the emergency department and at serial intervals for up to 7 days. Monoclonal antibody enzyme-linked immunosorbent assay kits to tumor necrosis factor (TNF), soluble TNF-receptor (sTNF-R) 55 kd and 75 kd, interleukin (IL)-1 and IL-1 RA, and IL-2 and IL-2r were used. Sera from 22 healthy individuals were used as normal controls. No TNF, IL-1, or IL-2 could be detected in any patient sera after injury. Control levels for the soluble cytokine receptors and RAs were as follows: sTNF-R 55 kd, 607 +/- 89 pg/mL; sTNF-R 75 kd, 2,141 +/- 169 pg/mL; IL-1 RA, 291 +/- 35 pg/mL; and IL-2r, 426 +/- 53 U/mL. In trauma patients, both 55 kd and 75 kd sTNF-R were significantly elevated on arrival to the emergency department, with values of 2,441 +/- 506 pg/mL (p < 0.001) and 4,736 +/- 537 pg/mL (p < 0.001), respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Surgery, University of California Irvine Medical Center, Orange 92668, USA.
authors.forename: M
authors.initials: M
authors.lastname: Cinat
authors.forename: K
authors.initials: K
authors.lastname: Waxman
authors.forename: N D
authors.initials: ND
authors.lastname: Vaziri
authors.forename: K
authors.initials: K
authors.lastname: Daughters
authors.forename: S
authors.initials: S
authors.lastname: Yousefi
authors.forename: G
authors.initials: G
authors.lastname: Scannell
authors.forename: G T
authors.initials: GT
authors.lastname: Tominaga
mesh_terms: D000328:Adult; D000368:Aged; D016207:Cytokines / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015601:Injury Severity Score; D008297:Male; D008875:Middle Aged; D018121:Receptors, Cytokine / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D012995:Solubility; D014947:Wounds and Injuries / Q000097:blood / Q000378:metabolism* / Q000401:mortality
chemical_list: D016207:Cytokines; D018121:Receptors, Cytokine