Isolation and characterization of a yeast gene, MPD1, the overexpression of which suppresses inviability caused by protein disulfide isomerase depletion.
MPD1, a yeast gene the overexpression of which suppresses the inviability caused by the loss of protein disulfide isomerase (PDI) was isolated and characterized. The MPD1 gene product retained a single disulfide isomerase active site sequence (APWCGHCK), an N-terminal putative signal sequence, and a C-terminal endoplasmic reticulum (ER) retention signal, and was a novel member of the PDI family. The gene product, identified in yeast extract, contained core size carbohydrates. MPD1 was not essential for growth, but overexpression of the gene suppressed the maturation defect of carboxypeptidase Y caused by PDI1 deletion, indicative of the related function to PDI in the yeast ER.
Tachikawa
H
H
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
Takeuchi
Y
Y
Funahashi
W
W
Miura
T
T
Gao
X D
XD
Fujimoto
D
D
Mizunaga
T
T
Onodera
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002268:Carboxypeptidases / Q000096:biosynthesis; D043402:Cathepsin A; D003001:Cloning, Molecular; D004721:Endoplasmic Reticulum / Q000737:chemistry; D005800:Genes, Fungal / Q000235:genetics*; D016153:Genes, Suppressor / Q000235:genetics*; D006031:Glycosylation; D007535:Isomerases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D019704:Protein Disulfide-Isomerases; D021382:Protein Sorting Signals / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment
D021382:Protein Sorting Signals; D002268:Carboxypeptidases; D043402:Cathepsin A; D007535:Isomerases; D019704:Protein Disulfide-Isomerases