Modifications of functional and physico-chemical properties of rat ileal plasma membranes.
A membrane fraction enriched with plasma membranes was isolated from rat ileal brush-border cells before and after five-day starvation of the animals. Cholesterol/phospholipid ratio of the standard cell membranes decreased highly significantly (0.42 to 0.18), as did the microviscosity of the membranes determined by polarization of fluorescence (0.187 to 0.142). Concomitantly, the specific activity of Na,K-ATPase in the basolateral membranes significantly increased (59 to 83 mumol ATP hydrolyzed per mg protein per min).
Gaggiotti
G
G
Unità Operativa di Chirurgia Generale, Terapia Nutrizionale INRCA, IRCCS, Ancona.
Taus
M
M
Spazzafumo
L
L
Tesei
M
M
La Rocca
R
R
Mazzanti
L
L
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D005454:Fluorescence Polarization; D007082:Ileum / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D008297:Male; D008560:Membrane Fluidity / Q000502:physiology; D008563:Membrane Lipids / Q000378:metabolism; D008871:Microvilli / Q000378:metabolism* / Q000502:physiology; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013217:Starvation / Q000378:metabolism*; D014783:Viscosity
D008563:Membrane Lipids; D010743:Phospholipids; D002784:Cholesterol; D000254:Sodium-Potassium-Exchanging ATPase