Expression of a functional fungal polyketide synthase in the bacterium Streptomyces coelicolor A3(2).
The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that it is possible to harness the molecular diversity of eukaryotic polyketide pathways by heterologous expression of biosynthetic genes in an easily manipulated model bacterial host in which prokaryotic aromatic and modular polyketide synthase genes are already expressed and recombined.
Bedford
D J
DJ
Department of Chemical Engineering, Stanford University, California 94305-5025, USA.
Schweizer
E
E
Hopwood
D A
DA
Khosla
C
C
D000595:Amino Acid Sequence; D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D008957:Models, Genetic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000096:biosynthesis / Q000235:genetics*; D010407:Penicillium / Q000201:enzymology / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012459:Salicylates / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*
D004271:DNA, Fungal; D009097:Multienzyme Complexes; D012459:Salicylates; C005236:6-methylsalicylic acid