H2-forming N5,N10-methylenetetrahydromethanopterin dehydrogenase: mechanism of H2 formation analyzed using hydrogen isotopes.
H2-forming N5,N10-methylenetetrahydromethanopterin dehydrogenase catalyzes the reversible dehydrogenation of N5,N10-methylenetetrahydromethanopterin (CH2 = H4MPT) to N5,N10-methenyltetrahydromethanopterin (CH = H4MPT+) and H2. In D2O both HD and D2 are formed from CH2 = H4MPT and in H2O both HD and H2 from CD2 = H4MPT. Evidence is presented that HD is not an intermediate in the formation of D2 and H2, respectively.
Klein
A R
AR
Laboratorium für Mikrobiologie, Philipps-Universität Marburg, Germany.
Fernández
V M
VM
Thauer
R K
RK
D017666:Deuterium Oxide / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D017014:Methanobacterium / Q000201:enzymology; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism*
D006859:Hydrogen; D000587:Oxidoreductases Acting on CH-NH Group Donors; C047126:methylenetetrahydromethanopterin dehydrogenase; D017666:Deuterium Oxide