Absence of high-affinity binding sites for interferon alpha/beta in variant murine CD4+ T lymphocytes not expressing the T cell antigen receptor.
The T cell antigen receptor complex (CD3/Ti) plays a role in specific antigen recognition as well as in signal transduction, with its surface expression required for the function of several other structurally distinct receptor systems, including CD2, Ly-6(TAP), and Thy-1. In this communication, evidence is presented suggesting an association between the surface expression of CD3/Ti and that of the type 1 interferon (IFN) receptor in a CD4+ murine T cell clone. We tested the proliferative responses and their capacity to be inhibited by type 1 IFN with the wild-type, CD3/Ti-positive T cell clone and its CD3/Ti-negative variants did not respond to specific antigen or anti-CD3 antibody stimulation but they did respond to T cell growth factor (TCGF), stimulation as did the wild-type parental cells. Therefore, the type 1 IFN inhibition of TCGF-stimulated proliferative responses of wild-type and variant cells were compared. Both natural and recombinant type 1 IFNs inhibited TCGF-induced tritiated thymidine (3H-TdR) incorporation in the wild-type T cell clone, with a ID50 of 60-80 U/ml. By contrast, the variants required much higher doses of type 1 IFN. The ID50 with natural murine IFN-beta was 10,000 U/ml, but this same dose of human IFN-alpha A/D gave only a marginal inhibitory effect. Accompanying the loss of IFN responsiveness, these variants also exhibited a loss of high-affinity type 1 IFN receptors. Taken together, these data suggest that the CD3/Ti complex plays a role in the surface expression of the type 1 IFN receptor in a CD4+ T cell clone.(ABSTRACT TRUNCATED AT 250 WORDS)
Shata
M T
MT
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201, USA.
Faltynek
C R
CR
Lewis
G K
GK
Kamin-Lewis
R M
RM
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry*; D002460:Cell Line; D007370:Interferon Type I / Q000097:blood*; D051379:Mice; D008805:Mice, Inbred A; D011948:Receptors, Antigen, T-Cell / Q000032:analysis*; D017471:Receptors, Interferon / Q000032:analysis*; D012016:Reference Values
D007370:Interferon Type I; D011948:Receptors, Antigen, T-Cell; D017471:Receptors, Interferon