Evaluation of a rapid microfiltration test for detection of HIV-1 antibody in serum.
BACKGROUND
Since the number of asymptomatic Human Immunodeficiency Virus Type 1 (HIV-1) infections has increased rapidly recently, to detect HIV-1 antibody rapidly becomes very critical when a quick mass survey is required or especially badly injured persons need blood transfusions immediately. This study evaluated sensitivity and specificity of a 10-minute qualitative assay (Single Used Diagnosis System; SUDS HIV-1 test) which is performed in a plastic cartridge using a modified enzyme immunoassay and to compare the results so obtained with more conventional HIV-1 antibody detection systems.


METHODS
A total number of 248 human serum samples collected at several local hospitals and health stations were delivered to this Institute from June 1993 to April 1994 for confirmation were used to compare results obtained by either particle agglutination (PA), Western blot, or the SUDS HIV-1 tests.


RESULTS
High sensitivity and specificity of SUDS were achieved using the conjugation of a specific gag antigen of HIV-1 and a synthetic HIV-1 envelop peptide. There was good correlation to one another among results from all the three tests.


CONCLUSIONS
Data from those three tests employed in this study revealed no significant differences in sensitivity and specificity. The simplicity and rapidity of the SUDS make it more suitable for use than the others, especially when an emergency room screening test or a blood bank test is required where urgent situations need urgent answers.
Wang
S Y
SY
National Institute of Preventive Medicine, Department of Health, Executive Yuan, Taipei, Taiwan, R.O.C.
Lin
T L
TL
Chen
H Y
HY
Horng
C B
CB
D000372:Agglutination Tests; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000097:blood*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D012680:Sensitivity and Specificity; D013997:Time Factors
D015483:HIV Antibodies