A member of the ClpB family of stress proteins is expressed during heat shock in Leishmania spp.
We have identified and isolated the Leishmania major homologue to the bacterial ClpB gene and to the yeast Hsp104 gene. ClpB in Leishmania major is a single-copy gene and encodes a low-abundance mRNA which is induced several-fold during a heat stress. We raised antibodies against the product of the recombinant gene and show that the leishmanial ClpB encodes a predominantly cytoplasmic protein of approx. 100 kDa which is detectable in Leishmania promastigotes of various species after exposure to elevated temperatures. We, therefore, term this protein Hsp100.
Hübel
A
A
Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Brandau
S
S
Dresel
A
A
Clos
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000737:chemistry; D049071:Endopeptidase Clp; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D015870:Gene Expression; D017125:Genes, Protozoan; D006360:Heat-Shock Proteins / Q000096:biosynthesis / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006358:Hot Temperature; D007150:Immunohistochemistry; D007891:Leishmania / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D016053:RNA, Protozoan / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014346:Trypanosoma brucei brucei / Q000235:genetics
D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C070972:HsP104 protein, S cerevisiae; D049071:Endopeptidase Clp; C069775:ClpB protein, E coli; C095049:ClpB protein, Leishmania