Rapid detection of Campylobacter jejuni and Campylobacter coli isolated from clinical specimens using the polymerase chain reaction.
Seventeen Campylobacter strains isolated from 16 children hospitalised with acute diarrhea were analysed by in vitro enzymatic amplification using two sets of oligonucleotide primers specific for Campylobacter jejuni and Campylobacter coli, respectively. Thirteen strains (76%) were identified as Campylobacter jejuni and four strains (24%) as Campylobacter coli. Subsequent bacteriological identification confirmed the identity of the same 13 Campylobacter jejuni strains and the 4 Campylobacter coli strains. Thus, these PCR methods enabled rapid and specific detection of all the Campylobacter jejuni and Campylobacter coli strains without any false-positive or false-negative results.
Stonnet
V
V
Laboratoire de Prédéveloppement des Sondes, Institut Pasteur, Paris, France.
Sicinschi
L
L
Mégraud
F
F
Guesdon
J L
JL
D001483:Base Sequence; D002169:Campylobacter Infections / Q000175:diagnosis*; D017000:Campylobacter coli / Q000302:isolation & purification*; D016123:Campylobacter jejuni / Q000302:isolation & purification*; D002648:Child; D004269:DNA, Bacterial / Q000032:analysis; D003967:Diarrhea / Q000382:microbiology*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial