Suppression of immune parameters in animal models of morphine dependence.
Implantation of pellets containing 75 mg of morphine induced short term (4 day) morphine dependence and markedly reduced total number of spleen cells of BALB/c mice, without affecting total body or liver weight. Polyclonal responses induced by anti-CD3 antibodies, Concanavalin A or Escherichia coli lipopolysaccharide in the remaining spleen cells of morphine-treated mice were also inhibited. Cytofluorimetric analysis indicated that the proportion of major functional lymphocyte populations (Ig+, CD3+, CD4+ and CD8+ lymphocytes) were not significantly changed in the spleen from morphine-dependent mice. Furthermore, expression levels of surface Ig, CD3, CD4, and CD8, were similar in spleen cells from control or morphine-treated mice. So, morphine dependence in BALB/c mice under these controlled conditions results in a specific defect in lymphoid cell number and function, with no incidence on body weight or particular lymphocyte subsets.
Portolés
J M
JM
Departamento de Nefrología, Hospital Clínico de San Carlos, Madrid, Spain.
Ojeda
G
G
Ronda
M
M
Leza
J C
JC
Rojo
J M
JM
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003864:Depression, Chemical; D004343:Drug Implants; D007153:Immunologic Deficiency Syndromes / Q000097:blood / Q000139:chemically induced* / Q000276:immunology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008099:Liver / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects; D018655:Lymphocyte Count / Q000187:drug effects*; D016131:Lymphocyte Subsets / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009020:Morphine / Q000494:pharmacology* / Q000633:toxicity; D009021:Morphine Dependence / Q000276:immunology*; D009929:Organ Size / Q000187:drug effects; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000473:pathology
D004343:Drug Implants; D007166:Immunosuppressive Agents; D011947:Receptors, Antigen, B-Cell; D009020:Morphine