gamma-Glutamyltransferase expression during all-trans retinoic acid-induced differentiation of hematopoietic cell lines.
gamma-Glutamyltransferase activity, genes transcripts and differentiation by all-trans retinoic acid have been investigated in cultured HL-60, U937, and K562 cells. Acquisition of morphological and functional characteristics confirmed the terminal differentiation of HL-60 and U937 cells. All-trans retinoic acid increased gamma-glutamyltransferase activity in a cell type- and time dependent manner. Treatments with all-trans retinoic acid isomers and structurally analogs showed that only retinoids with carboxylic acid group were able to induce enzyme activity in terminal differentiated cells. Additionally, the analysis of gamma-glutamyltransferase genes transcription products demonstrated clearly that, both in untreated and in RA treated cells, only mRNA type I transcribed from the gene 6, was expressed.
el Yaagoubi
M
M
Centre du Médicament. URA CNRS 597, Nancy, France.
Hachad
H
H
Leh
H
H
Siest
G
G
Wellman
M
M
D001483:Base Sequence; D001854:Bone Marrow Cells; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006098:Granulocytes / Q000166:cytology / Q000201:enzymology; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute; D007962:Leukocytes / Q000166:cytology* / Q000201:enzymology; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis; D014212:Tretinoin / Q000031:analogs & derivatives / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D005723:gamma-Glutamyltransferase / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D014212:Tretinoin; D005723:gamma-Glutamyltransferase