Chronic ethanol consumption affects cholinoceptor- and purinoceptor-mediated contractions of the isolated rat bladder.
Isolated bladder strips from 12-week ethanol-fed, pair-fed and control adult male rats were investigated. Contractile responses to carbachol (CCh; 0.1-300 microM) were statistically significantly potentiated in the ethanol-fed group compared to pair-fed and control. Contractions to beta,gamma-methylene ATP (beta,gamma-MeATP; 1-300 microM) were statistically significantly potentiated in the ethanol-fed group at the highest concentration tested (300 microM). Neurogenic contractions (0.5-32 pps) from the ethanol-fed group in the absence of atropine and after desensitisation by alpha,beta-methylene ATP (alpha,beta-MeATP; 3 microM), were significantly potentiated compared to the pair-fed and control groups; in the presence of atropine (1 microM), neurogenic contractions were significantly augmented at the higher frequencies. It is concluded that chronic ethanol treatment affects both cholinoceptor- and purinoceptor-mediated contractions of the rat bladder.
Knight
G E
GE
Department of Anatomy and Developmental Biology, University College London, UK.
Brizzolara
A L
AL
Soediono
P O
PO
Karoon
P
P
Burnstock
G
G
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000428:Alcohol Drinking; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009994:Osmolar Concentration; D051381:Rats; D017207:Rats, Sprague-Dawley; D011950:Receptors, Cholinergic / Q000502:physiology*; D011983:Receptors, Purinergic / Q000502:physiology*; D013997:Time Factors; D001743:Urinary Bladder / Q000187:drug effects / Q000502:physiology*
D011950:Receptors, Cholinergic; D011983:Receptors, Purinergic; D000255:Adenosine Triphosphate; D002217:Carbachol; C002630:alpha,beta-methyleneadenosine 5'-triphosphate