Purification and cloning of DNA fragments fractionated on agarose gels.
Purification of DNA fragments from acrylamide or agarose gels is a commonly used technique in the molecular biology laboratory. This article describes a rapid, efficient, and inexpensive method of purifying DNA fractions from an agarose gel. The purified DNA is suitable for use in a wide range of applications including ligation using DNA ligase. The procedure uses standard high-melting-temperature agarose and normal TBE electrophoresis buffer. In addition, the protocol does not involve the use of highly toxic organic solvents such as phenol.
Griffin
H G
HG
Genetics and Microbiology Department, Institute of Food Research, Colney, Norwich, UK.
Gasson
M J
MJ
D003001:Cloning, Molecular; D004247:DNA / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D013294:Lactococcus lactis
D004269:DNA, Bacterial; D004247:DNA