Imaging real-time neurite outgrowth and cytoskeletal reorganization with an atomic force microscope.
An atomic force microscope was used to image the morphology and structural reorganization of rat NIH/3T3 fibroblasts and PC-12 cells growing in petri dishes. NIH/3T3 fibroblasts had a uniform morphology and an extensive cytoskeletal network. Cell thickness varied from approximately 2-3 microns above the nucleus to approximately 20-30 nm over the distal processes, and cytoskeletal fibers as small as 30 nm wide were observed. Imaging over an extended period of time showed a limited degree of cytoskeletal reorganization. Localized force dissection did not induce significant retraction of cellular processes and immediate cell death. Differentiating PC-12 cells with a neuronal phenotype had a nonuniform morphology, abundant cytoskeletal elements, neuritic processes, and growth cones. The cell thickness varied from approximately 5-8 microns over the nucleus to approximately 100-500 nm over the neuritic processes; growth cones approximately 50-700 nm wide and end structures approximately 30-150 nm wide were visible. Repeated imaging showed reorganization of the growth cone, especially the appearance and disappearance of beadlike features and fibrous organization. Thus an atomic force microscope can be used for high-resolution real-time studies of the dynamic subcellular mechanisms that drive cell behavior.
Lal
R
R
Neuroscience Research Institute, University of California, Santa Barbara 93106, USA.
Drake
B
B
Blumberg
D
D
Saner
D R
DR
Hansma
P K
PK
Feinstein
S C
SC
D016475:3T3 Cells; D000818:Animals; D002454:Cell Differentiation; D003199:Computer Systems; D003599:Cytoskeleton / Q000502:physiology* / Q000648:ultrastructure*; D051379:Mice; D018625:Microscopy, Atomic Force; D016501:Neurites / Q000502:physiology* / Q000648:ultrastructure*; D016716:PC12 Cells; D051381:Rats