title: Brugia pahangi: effects of melanization on the uptake of nutrients by microfilariae in vitro.
abstract: The effects of melanization on nutrient uptake by the microfilariae of Brugia pahangi were investigated by using in vitro melanization and autoradiographic techniques. It was found that the melanotic capsules which formed around the microfilariae in vitro completely inhibited the uptake of the following radiolabeled nutrients into microfilarial tissues: D-[6-3H]glucose, L-[4,5-3H]leucine, [2-3H]glycine, [1-14C]linoleic acid, [5,6,8,9,11,12,14,15-3H]arachidonic acid, [2,8-3H]adenine, [8-14C]guanine HCl, and [5,6-3H]uracil. These results provide strong evidence that melanization may kill the microfilariae by starvation.
authors.affiliation: Department of Parasitology, School of Medicine, National Yang-Ming University, Taipei, Taiwan, Republic of China.
authors.forename: C C
authors.initials: CC
authors.lastname: Chen
authors.forename: C S
authors.initials: CS
authors.lastname: Chen
mesh_terms: D000225:Adenine / Q000378:metabolism; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D001692:Biological Transport; D017179:Brugia pahangi / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D002250:Carbon Radioisotopes; D002415:Cats; D003465:Culex / Q000469:parasitology; D005260:Female; D005947:Glucose / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006147:Guanine / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D008543:Melanins / Q000378:metabolism*; D008854:Microscopy, Electron; D011865:Radioisotope Dilution Technique; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D008041:Linoleic Acids; D008543:Melanins; D014316:Tritium; D016718:Arachidonic Acid; D006147:Guanine; D019787:Linoleic Acid; D007930:Leucine; D005947:Glucose; D000225:Adenine; D005998:Glycine