Phagocytic activity of FRTL-5 rat thyroid follicular cells as measured by ingestion of fluorescent latex beads.
A rat thyroid cell line (FRTL-5) was used to study the phagocytic activity of thyroid follicular cells using fluorescent latex beads and flow cytometric analysis. Morphologic studies demonstrated that latex beads were engulfed and located within cytoplasmic vacuoles of thyrocytes. Flow cytometric evaluation of cell suspensions revealed high levels of fluorescence in cells engulfing latex beads. Using thyrotropin (TSH) as a stimulator of thyroid function and human interleukin-1 beta as an inhibitor, protocols were established for measuring the effects of these substances on either basal or TSH-induced phagocytosis. Cells exposed to latex beads over time in basal (0H) or TSH-containing medium had an increase in time-dependent phagocytic activity which was maximal after 24 or 8 h, respectively. Treatment of FRTL-5 cells with either a stimulator or an inhibitor revealed maximal change in phagocytic activity after 72 h as measured by the percentage of phagocytic cells as well as the mean fluorescence intensity. Phagocytic activity and iodide trapping by FRTL-5 cells were qualitatively similar in both sensitivity and magnitude of change in the assays used in this study. Phagocytosis of fluorescent latex beads represents a sensitive nonradioactive assay of thyrocyte function whose regulation is similar to iodide trapping.
Ozaki
A
A
Tokushima Research Institute, Otsuka Pharmaceutical Company, Ltd., Japan.
Sagartz
J E
JE
Capen
C C
CC
D000818:Animals; D002460:Cell Line; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D007375:Interleukin-1 / Q000494:pharmacology; D007840:Latex; D008854:Microscopy, Electron; D010587:Phagocytosis; D051381:Rats; D013961:Thyroid Gland / Q000166:cytology* / Q000648:ultrastructure; D013972:Thyrotropin / Q000494:pharmacology
D005456:Fluorescent Dyes; D007375:Interleukin-1; D007840:Latex; D013972:Thyrotropin