Sequence and structure of Penicillium chrysogenum phoG, homologous to an acid phosphatase-encoding gene of Aspergillus nidulans.
A Penicillium chrysogenum (Pc) gene (phoG), homologous to an Aspergillus nidulans (An) gene which confers phosphate-non-repressible acid phosphatase (APase) activity, has been cloned and sequenced. The 2.9-kb genomic sequence corresponds to two ORFs of 149 and 1630 bp encoding a protein of 593 amino acids (aa). As verified by cDNA sequencing, the coding region is interrupted by an 85-bp intron. The deduced aa sequence of phoG reveals 61% aa identity to the translated long ORF of the An APase-encoding gene. Northern blot analysis indicated a 2.3-kb transcript in approximately equivalent amount in mycelia grown under different phosphate concentrations.
Marx
F
F
Institut für Mikrobiologie (Medizinische Fakultät), Universität Innsbruck, Austria.
Haas
H
H
Hofer
S
S
Stöffler
G
G
Redl
B
B
D000135:Acid Phosphatase / Q000235:genetics*; D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010408:Penicillium chrysogenum / Q000201:enzymology / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D000135:Acid Phosphatase