title: Defibrination essential in the assay of ionized magnesium in mononuclear cells.
abstract: Intracellular free (ionized) magnesium concentration was measured in mononuclear cells isolated from healthy volunteers by use of dual wavelength fluorescence (indicator: mag-fura-2). We found a free Mg2+ concentration of 1.28 +/- 0.08 mmol/l in mononuclear cells isolated from heparinized blood. When we defibrinated blood samples prior to the isolation step we measured 0.78 +/- 0.05 mmol/l of free Mg2+ in these cells. We conclude that this difference is caused by the platelets present in the heparinized specimens.
authors.affiliation: Afdeling Klinische Chemie van het Academisch Ziekenhuis Nijmegen, St. Radboud, Nijmegen, The Netherlands.
authors.forename: H L
authors.initials: HL
authors.lastname: Willems
authors.forename: J
authors.initials: J
authors.lastname: Kruse
authors.forename: W B
authors.initials: WB
authors.lastname: Geven
authors.forename: P
authors.initials: P
authors.lastname: Vis
authors.forename: L A
authors.initials: LA
authors.lastname: Monnens
mesh_terms: D001572:Benzofurans / Q000097:blood / Q000493:pharmacokinetics; D001792:Blood Platelets / Q000737:chemistry / Q000378:metabolism; D005337:Fibrin / Q000032:analysis*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D016257:Fura-2 / Q000031:analogs & derivatives*; D006801:Humans; D007477:Ions; D007963:Leukocytes, Mononuclear / Q000737:chemistry* / Q000378:metabolism; D008274:Magnesium / Q000097:blood*; D010080:Oxazoles / Q000097:blood / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence / Q000379:methods; D014343:Trypan Blue / Q000493:pharmacokinetics
chemical_list: D001572:Benzofurans; D005456:Fluorescent Dyes; D007477:Ions; D010080:Oxazoles; C058776:2-(2-(5-carboxy)oxazole)-5-hydroxy-6-aminobenzofuran-N,N,O-triacetic acid; D005337:Fibrin; D014343:Trypan Blue; D008274:Magnesium; D016257:Fura-2