title: Characterization of rat complement receptors and regulatory proteins. CR2 and Crry are conserved, and the C3b receptor of neutrophils and platelets is distinct from CR1.
abstract: In the mouse, CR1 and CR2 on B lymphocytes are encoded by alternatively spliced Cr2 gene transcripts. Immune adherence receptors that bind C3b are present on mouse platelets and unstimulated neutrophils, but they are not CR1. In this study, rabbit anti-mouse CR1/CR2 Ab immunoprecipitated a 145- to 150-kDa CR2 protein from rat platelets, neutrophils, and splenocytes, but not a approximately 200-kDa CR1 protein. By Northern analysis, cDNA for mouse CR2 hybridized to mRNA of 3.7 and 5.2 kb from both mouse and rat splenocytes. The murine decay-accelerating factor and membrane cofactor protein analogue Crry was present in rat platelets, neutrophils, E, and splenocytes as two distinct proteins of 65 to 70 kDa and 75 to 85 kDa. Rat platelets, neutrophils, and splenocytes contained a novel 200-kDa cell membrane protein that specifically bound to a rat C3b-Sepharose column. We have named this protein C3bR-200. C3bR-200 was not identified by anti-mouse CR1/CR2 or anti-human CR1 Ab. Rat E lacked C3bR-200. Rat neutrophils and splenocytes also contained an 80-kDa C3b-binding protein that was distinct from Crry, which we have named C3bR-80. Therefore, CR2 and Crry are present in the rat, and have similar qualities to those from the mouse, except that CR2 is located on rat platelets and neutrophils, which is not the case in the mouse. Rat platelets, neutrophils, and splenocytes have a 200-kDa C3b-binding protein, C3bR-200, that is likely to be the rodent immune adherence receptor.
authors.affiliation: Department of Medicine, University of Chicago, IL 60637, USA.
authors.forename: R J
authors.initials: RJ
authors.lastname: Quigg
authors.forename: V M
authors.initials: VM
authors.lastname: Holers
mesh_terms: D000818:Animals; D000954:Antigens, Surface; D001792:Blood Platelets / Q000378:metabolism*; D015152:Blotting, Northern; D002846:Chromatography, Affinity; D006801:Humans; D051379:Mice / Q000235:genetics / Q000378:metabolism; D008970:Molecular Weight; D009504:Neutrophils / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats / Q000235:genetics / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D011956:Receptors, Cell Surface; D011951:Receptors, Complement / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D017463:Receptors, Complement 3b / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D017464:Receptors, Complement 3d / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D013045:Species Specificity; D013154:Spleen / Q000166:cytology
chemical_list: D000954:Antigens, Surface; C081470:Cr1l protein, mouse; C513105:Cr1l protein, rat; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011951:Receptors, Complement; D017463:Receptors, Complement 3b; D017464:Receptors, Complement 3d