p53 protein detected by two different antibodies: relationship to proliferation and prognosis in acute lymphoblastic leukemia.
The expression of p53 protein was examined in a series of 69 children with acute lymphoblastic leukemia with two different monoclonal antibodies (Mab 1801 and 240). p53 expression was detected with at least one antibody in 49 cases (71%), whereas only 19 cases (27%) were positive with both antibodies. Variability in the immunostaining could be observed depending on the antibody used. Mab 240 gave the highest rate of positive staining (58%), followed by 1801 with 39%. Positive staining with 1801 was significantly associated with decreased proliferation of tumor cells as measured by Ki-67 labelling, indicating that possibly the wild-type p53 protein is preferably stained with this antibody. However, neither of the two antibodies has prognostic value or is correlated with histopathological parameters.
Mattern
J
J
German Cancer Research Center, Heidelberg.
Sauerbrey
A
A
Volm
M
M
D000367:Age Factors; D000911:Antibodies, Monoclonal; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D001853:Bone Marrow / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis; D009687:Nuclear Proteins / Q000032:analysis; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood* / Q000188:drug therapy / Q000401:mortality / Q000473:pathology; D011237:Predictive Value of Tests; D011379:Prognosis; D012189:Retrospective Studies; D015996:Survival Rate; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000096:biosynthesis / Q000097:blood
D000911:Antibodies, Monoclonal; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D016159:Tumor Suppressor Protein p53