title: Faecal interleukin-8 and tumour necrosis factor-alpha concentrations in cystic fibrosis.
abstract: Interleukin-8 (IL-8) and tumour necrosis factor-alpha (TNF-alpha) concentrations were measured in faecal samples from nine patients with cystic fibrosis and nine healthy age matched controls. The patients were assessed with Shwachman score, apparent energy absorption, pancreatic enzyme dosage, simple spirometry, and presence of pseudomonal colonisation. Median (range) wet stool IL-8 and TNF-alpha concentrations in patients were 32,113 pg/g (21,656-178,128) and 3187 pg/g (368-17,611) respectively, compared with < 43.5 pg (IL-8)/g (< 22-4079) and 99 pg (TNF-alpha)/g (< 0.26-231) in controls. IL-8 concentration was negatively correlated with Shwachman score (r = -0.79) and pancreatic enzyme dosage (r = -0.77), but not with energy absorption. Seven patients were mature enough to cooperate with spirometry. Their IL-8 concentrations correlated with percentage predicted forced expiratory volume in one second (r = -0.78). IL-8 concentration was greater in four patients with, than five without, established pseudomonal colonisation: median difference 134,583 pg/g. TNF-alpha concentration was not correlated with measures of disease severity. Faecal IL-8 concentration might reflect the severity of pulmonary inflammation in cystic fibrosis and could provide an easily obtainable marker of disease activity.
authors.affiliation: Department of Paediatric Medicine, Southampton General Hospital.
authors.forename: G L
authors.initials: GL
authors.lastname: Briars
authors.forename: T P
authors.initials: TP
authors.lastname: Dean
authors.forename: J L
authors.initials: JL
authors.lastname: Murphy
authors.forename: C J
authors.initials: CJ
authors.lastname: Rolles
authors.forename: J O
authors.initials: JO
authors.lastname: Warner
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000032:analysis; D002648:Child; D003550:Cystic Fibrosis / Q000188:drug therapy / Q000276:immunology* / Q000382:microbiology / Q000503:physiopathology; D005243:Feces / Q000737:chemistry*; D006801:Humans; D016209:Interleukin-8 / Q000032:analysis*; D008049:Lipase / Q000008:administration & dosage; D008168:Lung / Q000503:physiopathology; D010184:Pancreatic Extracts / Q000008:administration & dosage; D020799:Pancrelipase; D011552:Pseudomonas Infections / Q000150:complications; D013147:Spirometry; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: D015415:Biomarkers; D016209:Interleukin-8; D010184:Pancreatic Extracts; D014409:Tumor Necrosis Factor-alpha; D020799:Pancrelipase; D008049:Lipase