title: Alternative splicing of the human Shaker K+ channel beta 1 gene and functional expression of the beta 2 gene product.
abstract: Mammalian voltage-activated Shaker K+ channels associate with at least three cytoplasmic proteins: Kv beta 1, Kv beta 2 and Kv beta 3. These beta subunits contain variable N-termini, which can modulate the inactivation of Shaker alpha subunits, but are homologous throughout an aldo-keto reductase core. Human and ferret beta 3 proteins are identical with rat beta 1 throughout the core while beta 2 proteins are not; beta 2 also contains a shorter N-terminus and has no reported physiological role. We report that human beta 1 and beta 3 are derived from the same gene and that beta 2 modulates the inactivation properties of Kv1.4 alpha subunits.
authors.affiliation: Max-Planck-Institüt für experimentelle Medizin, Abteilung 11, Göttingen, Germany.
authors.forename: K
authors.initials: K
authors.lastname: McCormack
authors.forename: T
authors.initials: T
authors.lastname: McCormack
authors.forename: M
authors.initials: M
authors.lastname: Tanouye
authors.forename: B
authors.initials: B
authors.lastname: Rudy
authors.forename: W
authors.initials: W
authors.lastname: Stühmer
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D017124:Conserved Sequence; D017931:DNA Primers; D005260:Female; D005289:Ferrets; D015870:Gene Expression; D015186:Hominidae / Q000235:genetics*; D006801:Humans; D046911:Macromolecular Substances; D008322:Mammals; D008564:Membrane Potentials / Q000502:physiology; D008969:Molecular Sequence Data; D009206:Myocardium / Q000378:metabolism; D009865:Oocytes / Q000502:physiology; D010452:Peptide Biosynthesis; D010455:Peptides / Q000235:genetics*; D016133:Polymerase Chain Reaction; D051381:Rats; D017386:Sequence Homology, Amino Acid; D051637:Shaker Superfamily of Potassium Channels; D014162:Transfection; D014982:Xenopus laevis
chemical_list: D017931:DNA Primers; D046911:Macromolecular Substances; D010455:Peptides; C076413:Shaker B potassium channel polypeptide; D051637:Shaker Superfamily of Potassium Channels