Sex steroids up-regulate E-cadherin expression in hormone-responsive LNCaP human prostate cancer cells.
There is convincing evidence that a reduced expression of the E-cadherin cell-cell adhesion molecule associates with low tumor grade and poor prognosis in prostate cancer patients. However, little is known on how E-cadherin levels are regulated in human prostate cancer cells. We have inspected the effect of both androgens and estrogen on the expression of E-cadherin in the hormone-responsive LNCaP prostate tumor cell line, which is endowed with both androgen and estrogen receptors. Using both Dot Blot analysis and immunocytochemistry we have observed that either steroid significantly increased E-cadherin levels in these cells; this effect was not reversed by the simultaneous addition of the relevant antagonist, hydroxyflutamide or ICI-182,780.
Carruba
G
G
Hormone Biochemistry Laboratory, University Medical School, Palermo, Italy.
Miceli
D
D
D'Amico
D
D
Farruggio
R
R
Comito
L
L
Montesanti
A
A
Polito
L
L
Castagnetta
L A
LA
D000726:Androgen Antagonists / Q000494:pharmacology; D015820:Cadherins / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000494:pharmacology; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D005485:Flutamide / Q000031:analogs & derivatives / Q000494:pharmacology; D000077267:Fulvestrant; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D006801:Humans; D015151:Immunoblotting; D007150:Immunohistochemistry; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism*
D000726:Androgen Antagonists; D015820:Cadherins; D004965:Estrogen Antagonists; D012739:Gonadal Steroid Hormones; D013196:Dihydrotestosterone; D000077267:Fulvestrant; C014290:hydroxyflutamide; D004958:Estradiol; D005485:Flutamide