Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes.
We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZ alpha-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.
West
S E
SE
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706, USA.
Romero
M J
MJ
Regassa
L B
LB
Zielinski
N A
NA
Welch
R A
RA
D016977:Actinobacillus pleuropneumoniae / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003001:Cloning, Molecular / Q000379:methods*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D006190:Haemophilus / Q000235:genetics; D016978:Mannheimia haemolytica / Q000235:genetics; D010641:Phenotype; D010957:Plasmids; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D013045:Species Specificity; D001616:beta-Galactosidase / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D001616:beta-Galactosidase