Characterization of three new snRNAs from Saccharomyces cerevisiae: snR34, snR35 and snR36.
Genes for three novel snRNAs of Saccharomyces cerevisiae have been isolated, sequenced and tested for essentiality. The RNAs encoded by these genes are designated snR34, snR35 and snR36 respectively and contain 203, 204 and 182 nucleotides. Each RNA is derived from a single copy gene and all three RNAs are believed to be nucleolar, i.e. snoRNAs, based on extraction properties and association with fibrillarin. SnR34 and snR35 contain a trimethylguanosine cap, but this feature is absent from snR36. The novel RNAs lack elements conserved among several other snoRNAs, including box C, box D and long sequence complementarities with rRNA. Genetic disruption analyses showed each of the RNAs to be dispensable and a haploid strain lacking all three RNAs and a previously characterized fourth snoRNA (snR33) is also viable. No differences in the levels of precursors or mature rRNAs were apparent in the four gene knock-out strain. Possible roles for the new RNAs in ribosome biogenesis are discussed.
Samarsky
D A
DA
Department of Biochemistry and Molecular Biology, Lederle Graduate Research Center, University of Massachusetts, Amherst 01002, USA.
Balakin
A G
AG
Fournier
M J
MJ
D000483:Alleles; D001483:Base Sequence; D015152:Blotting, Northern; D002466:Cell Nucleolus / Q000737:chemistry; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004262:DNA Restriction Enzymes; D007163:Immunosorbent Techniques; D008969:Molecular Sequence Data; D016296:Mutagenesis; D012315:RNA Caps / Q000032:analysis; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000032:analysis; D012342:RNA, Small Nuclear / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D017385:Sequence Homology
D002868:Chromosomal Proteins, Non-Histone; D012315:RNA Caps; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012342:RNA, Small Nuclear; C047458:fibrillarin; D004262:DNA Restriction Enzymes