Repression of transcriptional activity by heterologous KRAB domains present in zinc finger proteins.
We report the characterization of three novel members of the KRAB-domain containing C2-H2 zinc finger family (ZNF133, 136 and 140). KRAB (Krüppel-associated box) is an evolutionarily conserved protein domain found N-terminally with respect to the zinc finger repeats that encodes the DNA binding domain. ZNF133 and ZNF140 have both the KRAB A- and KRAB B-boxes present at their N-terminus, whereas ZNF136 contains only the KRAB A-box. We have previously demonstrated that the KRAB domains derived from ZNF133 and ZNF140 are potent transcriptional repression domains [Margolin et al. (1994) Proc. Natl. Acad. Sci. USA 91, 4509-4513]. The KRAB domain from ZNF136, containing only subdomain A, is a considerable weaker suppression domain; however, when fused to the heterologous KRAB B subdomain of ZNF10 (KOX1) the two subdomains from a KRAB domain which induces repression as potently as previously reported KRAB domains.
Vissing
H
H
Basel Institute for Immunology, Switzerland.
Meyer
W K
WK
Aagaard
L
L
Tommerup
N
N
Thiesen
H J
HJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics*; D006801:Humans; D007535:Isomerases / Q000097:blood / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D019704:Protein Disulfide-Isomerases; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012097:Repressor Proteins / Q000097:blood / Q000737:chemistry / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; C095224:ZNF133 protein, human; C095225:ZNF136 protein, human; C095226:ZNF140 protein, human; C095229:MPD1 protein, S cerevisiae; D007535:Isomerases; D019704:Protein Disulfide-Isomerases