title: Transient expression of a Drosophila melanogaster hsp70 promoter/lacZ construct injected into larvae of two species of predatory mites (Acari: Phytoseiidae).
abstract: The Drosophila melanogaster heat shock 70 promoter (hsp70) was used to regulate expression of the Escherichia coli beta-galactosidase gene (lacZ) in transiently-transformed predatory mite larvae. A construct containing the hsp70 promoter upstream of the D. melanogaster alcohol dehydrogenase (adh) translational start site and Escherichia coli lacZ gene fusion (adh/lacZ) was injected into larvae of Metaseiulus occidentalis and Amblyseius finlandicus. LacZ expression was compared to expression of a similar construct lacking any upstream regulatory sequence. Expression from the hsp70 promoter was strong and heat shock-dependent in both species. The Drosophila hsp70 promoter therefore appears useful for regulating expression of exogenous DNA in both phytoseiid species and may be broadly applicable in the Phytoseiidae. Furthermore, the lacZ gene is a useful gene for analysis of expression in both species. Larval microinjection provides a method of assessing transient expression and of examining native regulatory sequences in these two phytoseiids and will likely be useful in other phytoseiid mites with only minor modifications.
authors.affiliation: Department of Entomology, University of California, Berkeley, USA.
authors.forename: J K
authors.initials: JK
authors.lastname: Presnail
authors.forename: M A
authors.initials: MA
authors.lastname: Hoy
mesh_terms: D000818:Animals; D004032:Diet; D004331:Drosophila melanogaster / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D007763:Lac Operon; D007814:Larva / Q000235:genetics / Q000378:metabolism; D008845:Microinjections; D008925:Mites / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014170:Transformation, Genetic; D001616:beta-Galactosidase / Q000235:genetics
chemical_list: D018840:HSP70 Heat-Shock Proteins; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase