Structure, organization and promoter expression of the actin-encoding gene in Trichoderma reesei.
The single gene encoding actin (Act) in the cellulolytic filamentous fungus Trichoderma reesei (Tr) has been isolated and characterized. The gene contains five introns located in identical positions when compared to the putative ancestral actin genes (act) present in Thermomyces lanuginosus and Aspergillus nidulans. The 5' untranslated region (UTR) of the gene contains a TATA-like sequence (TAATA), a C + T-rich region and a potential CCAAT motif. This region was used as a homologous promoter to direct expression of hygromycin-B-resistance-encoding gene as a dominant-selectable Tr marker.
Matheucci
E
E
Department of Biochemistry, University of São Paulo, Brazil.
Henrique-Silva
F
F
el-Gogary
S
S
Rossini
C H
CH
Leite
A
A
Vera
J E
JE
Urioste
J C
JC
Crivellaro
O
O
el-Dorry
H
H
D000199:Actins / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D004274:DNA, Recombinant; D004352:Drug Resistance, Microbial / Q000235:genetics; D006921:Hygromycin B / Q000494:pharmacology; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014242:Trichoderma / Q000187:drug effects / Q000235:genetics*
D000199:Actins; D017931:DNA Primers; D004274:DNA, Recombinant; D006921:Hygromycin B