Dual-specificity interaction of HIV-1 TAR RNA with Tat peptide-oligonucleotide conjugates.
An oligonucleotide-peptide conjugate, having dual binding capability for a designated RNA, was designed. The peptide portion of the conjugate interacts with a folded domain in the RNA, whereas the oligonucleotide portion hybridizes with a nearby single-stranded region in the RNA. The dual specificity was proven in a model HIV-1 TAR RNA system using an RNase H cleavage assay to assess antisense binding to this RNA. The peptide portion of the conjugate was shown to confer increased specificity on the oligonucleotide.
Tung
C H
CH
Center for Advanced Biotechnology and Medicine, Piscataway, New Jersey 08854, USA.
Wang
J
J
Leibowitz
M J
MJ
Stein
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D015696:Gene Products, tat / Q000737:chemistry*; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D010455:Peptides / Q000737:chemistry / Q000378:metabolism; D012367:RNA, Viral / Q000737:chemistry* / Q000378:metabolism; D054322:tat Gene Products, Human Immunodeficiency Virus
D015696:Gene Products, tat; D009841:Oligonucleotides; D010455:Peptides; D012367:RNA, Viral; D054322:tat Gene Products, Human Immunodeficiency Virus