title: Thermodynamics of interaction of the fusion-inhibiting peptide Z-D-Phe-L-Phe-Gly with dioleoylphosphatidylcholine vesicles: direct calorimetric determination.
abstract: The binding of the fusion-inhibiting peptide Z-D-Phe-L-Phe-Gly to unilamellar lipid vesicles of dioleoylphosphatidylcholine (DOPC) was investigated by isothermal titration calorimetry (ITC). The peptide Z-D-Phe-L-Phe-Gly is known to inhibit fusion of myxo- and paramyxoviruses with cells as well as cell-cell and vesicle-vesicle fusion in model systems. Calorimetric titrations conducted over a range of temperatures permitted characterization of the thermodynamics of the interaction of Z-D-Phe-L-Phe-Gly with model DOPC lipid membranes. Simultaneous global analysis of 15 ITC binding curves acquired at four different temperatures allowed determination of the equilibrium site association constant (K), stoichiometry of binding (n), binding enthalpy change (delta H), and heat capacity change of binding (delta Cp) in a single set of experiments. The binding affinity and enthalpy change per mole of DOPC bound at 25 degrees C was log K = 2.463 +/- 0.075 and delta H = -1.07 +/- 0.12 kcal/mol DOPC while the binding heat capacity change per mole of DOPC bound was delta Cp = -20.3 +/- 2.8 cal/(K.mol DOPC) with a temperature dependence (from 10-45 degrees C) of d(delta Cp)/dT = 0.37 +/- 0.18 cal/(K2.mol DOPC). A temperature-independent binding stoichiometry was determined to be n = 5.56 +/- 0.33 DOPC molecules per Z-D-Phe-L-Phe-Gly. A comparison of these results with previous peptide-lipid binding studies is discussed as is their relevance to a current model of the interaction of fusion-inhibiting peptides with phospholipid membranes.
authors.affiliation: Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USA.
authors.forename: D C
authors.initials: DC
authors.lastname: Turner
authors.forename: M
authors.initials: M
authors.lastname: Straume
authors.forename: M R
authors.initials: MR
authors.lastname: Kasimova
authors.forename: B P
authors.initials: BP
authors.lastname: Gaber
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry* / Q000494:pharmacology; D002151:Calorimetry; D007700:Kinetics; D008051:Lipid Bilayers; D008433:Mathematics; D008561:Membrane Fusion / Q000187:drug effects; D008958:Models, Molecular; D008962:Models, Theoretical; D008968:Molecular Conformation; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology; D009975:Orthomyxoviridae / Q000187:drug effects; D010713:Phosphatidylcholines; D011487:Protein Conformation; D018108:Respirovirus / Q000187:drug effects; D013816:Thermodynamics
chemical_list: D000998:Antiviral Agents; D008051:Lipid Bilayers; D009842:Oligopeptides; D010713:Phosphatidylcholines; C044920:phenylalanyl-phenylalanyl-glycine; C017251:1,2-oleoylphosphatidylcholine