title: Improved method for polyamine determination in TMV, a rod-shaped virus.
abstract: Polyamines were measured in viruses using different techniques. An improved method of polyamine analysis is reported for tobacco mosaic virus (TMV), a rod-shaped virus (95% protein and 5% RNA), based on HPLC of sonicated PCA-treated highly purified virus suspensions. This method allowed higher and more reliable recovery of TMV polyamines (putrescine, spermidine and spermine) when compared to the HPLC of non-sonicated samples and to thin layer chromatography. It is suggested that sonication acts on PCA-precipitated protein aggregates causing the release of trapped polyamine molecules.
authors.affiliation: Dipartimento di Biologia evoluzionistica sperimentale, University of Bologna, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Torrigiani
authors.forename: A L
authors.initials: AL
authors.lastname: Rabiti
authors.forename: L
authors.initials: L
authors.lastname: Betti
authors.forename: F
authors.initials: F
authors.lastname: Marani
authors.forename: M
authors.initials: M
authors.lastname: Brizzi
authors.forename: N
authors.initials: N
authors.lastname: Bagni
authors.forename: A
authors.initials: A
authors.lastname: Canova
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010472:Perchlorates / Q000494:pharmacology; D011073:Polyamines / Q000032:analysis*; D011700:Putrescine / Q000032:analysis; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis; D014027:Tobacco Mosaic Virus / Q000737:chemistry* / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D010472:Perchlorates; D011073:Polyamines; D013096:Spermine; D013095:Spermidine; D011700:Putrescine