title: Adrenotensin: an ADM gene product with the opposite effects of ADM.
abstract: The purpose of the present study was to investigate the effects of putative products of the ADM gene, other than ADM including, prodepin, proADM45-92 and proADM153-185 on cat pulmonary arterial (PA) rings with or without precontraction with U46619. Addition of proADM153-185 (3 x 10(-10)-10(-6) M) increased tension in a concentration-dependent manner in cat PA rings without precontraction. When vessels were precontracted with U46619, ADM(3 x 10(-10)-10(-6) M) produced a concentration-dependent vasorelaxant response, whereas proADM153-185 produced a weak concentration-dependent contractile response. Prodepin and proADM45-92 up to 10(-6)M had no activity on PA rings. Since proADM153-185, similar to ADM, would be expected to be released in free form following endopeptidase-induced cleavage, the present data suggest proADM undergoes proteolytic processing to release peptides with divergent vascular effects. Thus, the present data also suggest that proADM153-185 may represent a novel product of the ADM gene and term this putative new substance "adrenotensin".
authors.affiliation: Department of Surgery, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA.
authors.forename: B
authors.initials: B
authors.lastname: Gumusel
authors.forename: J K
authors.initials: JK
authors.lastname: Chang
authors.forename: A
authors.initials: A
authors.lastname: Hyman
authors.forename: H
authors.initials: H
authors.lastname: Lippton
mesh_terms: D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D053607:Adrenomedullin; D000818:Animals; D002415:Cats; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010446:Peptide Fragments / Q000494:pharmacology*; D010455:Peptides / Q000235:genetics / Q000494:pharmacology*; D011450:Prostaglandin Endoperoxides, Synthetic / Q000494:pharmacology; D011498:Protein Precursors / Q000494:pharmacology*; D011651:Pulmonary Artery / Q000187:drug effects / Q000502:physiology; D013928:Thromboxane A2 / Q000031:analogs & derivatives / Q000494:pharmacology; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D010446:Peptide Fragments; D010455:Peptides; D011450:Prostaglandin Endoperoxides, Synthetic; D011498:Protein Precursors; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; C094998:adrenomedullin precursor (45-92); C094997:adrenotensin; D053607:Adrenomedullin; C087423:prodepin; D013928:Thromboxane A2; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid