title: Measuring plasma fibrinogen levels in patients with liver cirrhosis. The occurrence of proteolytic fibrin(ogen) degradation products and their influence on several fibrinogen assays.
abstract: In patients with liver cirrhosis the fibrinogen molecule is under constant attack of various proteolytic enzymes, which might affect results of the different assay systems for fibrinogen. We therefore studied the measurement of fibrinogen in the plasma of patients with mild, moderate and severe cirrhosis of the liver. Fibrinogen levels were measured with the Clauss method (functional fibrinogen); an enzyme immuno assay (EIA) for HMW + L MW fibrinogen; and an assay that measures the total clottable fibrinogen. With all three methods we found normal or slightly increased fibrinogen levels in patients with mild or moderate cirrhosis, whereas patients with severe cirrhosis had decreased levels. No evidence was found for increased partial fibrinogen proteolysis, resulting in increases of LMW'-fibrinogen in cirrhotic patients. We further observed that fibrinogen degradation products levels increased slightly with the severity of the disease, but were still in the normal range in patients with severe cirrhosis. This indicates a very low level of primary fibrinolysis. Fibrin degradation products levels increased much stronger, which points to intravascular coagulation. The levels of the fibrin degradation products remained below the level where they are expected to influence the Clauss assay. In patients with liver cirrhosis the measurement of plasma fibrinogen levels with the three studied methods give comparable results. We suggest to apply the Clauss assay in cirrhotic patients because of this and because it has good reproducibility and because the test is cheap, quick and easy to perform.
authors.affiliation: Dept. Internal Medicine II, Erasmus University Rotterdam, The Netherlands.
authors.forename: M P
authors.initials: MP
authors.lastname: de Maat
authors.forename: W
authors.initials: W
authors.lastname: Nieuwenhuizen
authors.forename: E A
authors.initials: EA
authors.lastname: Knot
authors.forename: H R
authors.initials: HR
authors.lastname: van Buuren
authors.forename: G R
authors.initials: GR
authors.lastname: Swart
mesh_terms: D002648:Child; D005337:Fibrin / Q000378:metabolism; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis*; D005340:Fibrinogen / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood*
chemical_list: D005338:Fibrin Fibrinogen Degradation Products; D005337:Fibrin; D005340:Fibrinogen