The structure of polyamine analogues determines haemoglobin production and cytotoxicity in murine erythroleukaemia cells.
The naturally occurring polyamine spermine induces haemoglobin synthesis in murine erythroleukaemia (MEL) cells. We have studied the ability of various polyamine analogues to inhibit cell growth and induce haemoglobin production. Polyamine analogues with free terminal amino groups were good inducers of haemoglobin production in MEL cells. Haemoglobin levels correlated with the number of positive charges: pentamines (five positive charges) were stronger inducers than tetramines (four positive charges). Compounds ethylated at their terminal amines were poor inducers of haemoglobin production but good inhibitors of MEL cell growth. These results provide evidence that polyamine analogues support specific biological functions of polyamines in MEL cells and suggest relationships between polyamine structure and function.
Clément
S
S
Laboratoire d'Immunochimie INSERM C.J.F. 89-05, Faculté de Médecine Lyon Sud, Oullins, France.
Delcros
J G
JG
Basu
H S
HS
Quash
G
G
Marton
L J
LJ
Feuerstein
B G
BG
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006454:Hemoglobins / Q000096:biosynthesis*; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D011073:Polyamines / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
D006454:Hemoglobins; D011073:Polyamines