Tumor necrosis factor expression during mouse hepatitis virus-induced demyelinating encephalomyelitis.
Neutralizing anti-tumor necrosis factor alpha (TNF-alpha) antibody treatment of mice infected with the neurotropic JHMV strain of mouse hepatitis virus showed no reduction of either virus-induced encephalomyelitis or central nervous system demyelination. TNF-alpha-positive cells were present in the central nervous system during infection; however, TNF-alpha could not be colocalized with JHMV-infected cells. In vitro, TNF-alpha mRNA rapidly accumulated following JHMV infection; however, no TNF-alpha was secreted because of inhibition of translation. Both live and UV-inactivated virus inhibited TNF-alpha secretion induced by lipopolysaccharide. These data show that TNF-alpha is not secreted from infected cells and indicate that if contributes to either JHMV-induced acute encephalomyelitis nor primary demyelination.
Stohlman
S A
SA
Department of Neurology, University of Southern California School of Medicine, Los Angeles 90033, USA.
Hinton
D R
DR
Cua
D
D
Dimacali
E
E
Sensintaffar
J
J
Hofman
F M
FM
Tahara
S M
SM
Yao
Q
Q
D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D003711:Demyelinating Diseases / Q000473:pathology / Q000503:physiopathology* / Q000821:virology; D004679:Encephalomyelitis / Q000473:pathology / Q000503:physiopathology* / Q000821:virology; D015870:Gene Expression; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000473:pathology / Q000502:physiology / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D006517:Murine hepatitis virus / Q000302:isolation & purification / Q000472:pathogenicity* / Q000528:radiation effects; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D013045:Species Specificity; D013116:Spinal Cord / Q000473:pathology* / Q000503:physiopathology / Q000821:virology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D014466:Ultraviolet Rays
D000906:Antibodies; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha