Lack of significant promoting activity by benzene in the rat liver model of carcinogenesis.
The promoting activity of benzene on rat liver carcinogenesis was investigated. The chemical was tested for its ability to enhance the growth of preneoplastic foci, as detected by gamma-glutamyl transpeptidase (GGT) staining in diethylnitrosamine (DENA) initiated hepatocytes. Two weeks after receiving a single ip dose of 200 mg/kg DENA, F344 rats were given daily oral doses of 400 mg/kg benzene (5 d/wk) for 6 wk. At wk 3 after the experiment began, all animals underwent partial hepatectomy, and at wk 8 were sacrificed. Following benzene treatment, no variation in the liver/body weight ratio was observed. After scoring of foci in liver slides, no significant difference in foci number and area could be observed between rats treated with DENA plus benzene and rats treated with DENA alone. Practically no foci were observed in the liver of rats treated only with benzene. The lack of benzene promoting activity in the liver model is discussed.
Taningher
M
M
Istituto Nazionale per la Ricerca sul Cancro (IST), Genoa, Italy.
Perrotta
A
A
Malacarne
D
D
Parodi
S
S
Zedda
A I
AI
Colacci
A
A
Grilli
S
S
D000818:Animals; D001554:Benzene / Q000494:pharmacology*; D004052:Diethylnitrosamine; D004195:Disease Models, Animal; D004347:Drug Interactions; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced* / Q000473:pathology; D008297:Male; D011230:Precancerous Conditions / Q000139:chemically induced* / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344
D004052:Diethylnitrosamine; D001554:Benzene