Enantioselective determination of chiral 2,2',3,3',4,6'-hexachlorobiphenyl (PCB 132) in human milk samples by multidimensional gas chromatography/electron capture detection and by mass spectrometry.
The enantiomeric ratio (ER) of the chiral polychlorinated biphenyl (PCB) 132 (2,2',3,3',4,6'-hexachlorobiphenyl) was determined in human milk samples. The enantiomers were separated by multidimensional gas chromatography (MDGC), using different achiral columns for pre-separation and a Chirasil-Dex column for enantiomer separation, and quantified by electron-capture detection (ECD) and by mass spectrometry (MS/SIM). The determined enantiomeric ratios varied between 0.45-0.85 (enantiomeric excess: 38-8%).
Glausch
A
A
Institut für Organische Chemie, Universität Tübingen, Germany.
Hahn
J
J
Schurig
V
V
D002849:Chromatography, Gas; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008895:Milk, Human / Q000737:chemistry*; D008970:Molecular Weight; D011078:Polychlorinated Biphenyls / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D012015:Reference Standards; D013237:Stereoisomerism
C094859:2,2',3,3',4,6'-hexachlorobiphenyl; D011078:Polychlorinated Biphenyls