title: Mitochondrial deoxyribonucleic acid deletions in oocytes and reproductive aging in women.
abstract: OBJECTIVE
  To determine whether oocytes from women harbor deletions in mitochondrial DNA (mtDNA) and whether deleted mtDNA is more common in oocytes from older women than oocytes from younger women.
  
  
  METHODS
  A polymerase chain reaction (PCR)-based strategy, which depends on deletions approximating otherwise widely separated primers to demonstrate mtDNA deletions in individual oocytes, was used.
  
  
  METHODS
  Yale In Vitro Fertilization Clinic and Laboratory at Yale University School of Medicine.
  
  
  METHODS
  Primers flanked a region of the mitochondrial genome in which long direct repeated sequence predispose to deletions. The primers identified the 0.5-kb "common" deletion. Deleted mtDNA was represented by a 0.5-kb band when primers separated by 5 kb were used. Control reactions used primers that amplify mtDNA outside the deletion hotspot. Positive controls included brain and/or muscle from aged individuals, and negative controls included fetal tissue and DNA-free blanks. Nested primers confirmed the specificity of the deleted product.
  
  
  RESULTS
  Unfertilized oocytes, muscle, and brain tissue contained PCR products consistent with deleted mtDNA. Fetal tissue lacked the mtDNA deletion product. Deleted mtDNA was detected in single oocytes. Oocytes from older women were more likely to contain deleted mtDNA than oocytes from younger women.
  
  
  CONCLUSIONS
  Deleted mtDNA in unfertilized oocytes may serve as a marker of oocyte senescence.
authors.affiliation: Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Keefe
authors.forename: T
authors.initials: T
authors.lastname: Niven-Fairchild
authors.forename: S
authors.initials: S
authors.lastname: Powell
authors.forename: S
authors.initials: S
authors.lastname: Buradagunta
mesh_terms: D000328:Adult; D000375:Aging; D001923:Brain Chemistry; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D005260:Female; D005307:Fertilization in Vitro; D017353:Gene Deletion; D006801:Humans; D008297:Male; D009132:Muscles / Q000737:chemistry; D009865:Oocytes / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D012098:Reproduction / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial