Genotyping of hepatitis C virus in South Africa.
The six major hepatitis C virus genotypes were investigated by using samples from 79 seropositive and PCR-positive blood donors from three different regions of South Africa as well as 9 patients with chronic renal failure, 19 with liver disease, and 23 with hemophilia. PCR products of the genome were typed by restriction fragment length polymorphic analysis by RsaI-HaeIII and MvaI-HinfI double digestion. Type 5 occurred in 40% of this population group; type 1 occurred in 33%; and types 2, 3, and 4 were found in 13.8, 7.7, and 2.3%, respectively.
Smuts
H E
HE
Department of Medical Microbiology, University of Cape Town Medical School, Observatory, South Africa.
Kannemeyer
J
J
D004279:DNA, Viral / Q000097:blood / Q000235:genetics; D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000453:epidemiology / Q000821:virology; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013019:South Africa / Q000453:epidemiology
D004279:DNA, Viral