title: Protective effect of Brucella outer membrane complex-bearing liposomes against experimental murine brucellosis.
abstract: Liposomes of stable multilamellar type, which previously demonstrated great efficiency in antibiotic transport, were used in this study as transport vehicles of antigenic extracts of Brucella melitensis (HS: complex of lipopolysaccharide/phospholipids/outer membrane proteins). The incorporation of HS into positively charged liposomes produced a protective effect against experimental murine brucellosis when they were administered 1 day before or 2 days after infection, as the number of colony-forming units in the spleen was reduced in relation to the untreated control group (P < 0.01). On the other hand, the use of HS-free or bound in liposomes with negative net charge did not produce a significant effect. Moreover, the incorporation of HS into cationic liposomes eliminated the toxicity of the lipopolysaccharide.
authors.affiliation: Dpto. de Microbiología, Universidad de Navarra, Pamplona, Spain.
authors.forename: A I
authors.initials: AI
authors.lastname: Vitas
authors.forename: R
authors.initials: R
authors.lastname: Diaz
authors.forename: C
authors.initials: C
authors.lastname: Gamazo
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage*; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage* / Q000276:immunology; D002006:Brucellosis / Q000517:prevention & control*; D004337:Drug Carriers; D005260:Female; D008070:Lipopolysaccharides / Q000008:administration & dosage* / Q000276:immunology; D008081:Liposomes / Q000008:administration & dosage*; D051379:Mice; D010743:Phospholipids / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D004337:Drug Carriers; D008070:Lipopolysaccharides; D008081:Liposomes; D010743:Phospholipids