Effect of scorpion toxin from Tityus serrulatus on the contraction of the isolated rat uterus.
Scorpion toxin T1 from Tityus serrulatus was tested for its effects on the isolated rat uterus preparation. T1 (5 micrograms/ml) caused a contraction of the uterus, which was potentiated by neostigmine (1.64 x 10(-6) M) and abolished by atropine (1.4 x 10(-7) M). After addition of neostigmine to the bath, we noted a higher amplitude of the toxin-induced contractions, and the appearance of repetitive rhythmic contractions. The scorpion toxin-induced contraction was not prevented by previous addition to the bath of hexamethonium or bradykinin, 5-HT and angiotensin II antagonists. The uterine contraction was prevented by previous addition to the bath of either tetrodotoxin (5 x 10(-8) M) or lidocaine (4.2 x 10(-5) M). These data seem to indicate that scorpion toxin-induced rat uterus contractions are due to actions on post-ganglionic autonomic nerve endings, with acetylcholine release and stimulation of muscarinic receptors.
Mendonça
M
M
Departamento de Ginecologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Da Luz
M M
MM
Freire-Maia
L
L
Cunha-Melo
J R
JR
D000109:Acetylcholine / Q000378:metabolism; D000804:Angiotensin II / Q000037:antagonists & inhibitors; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001920:Bradykinin / Q000037:antagonists & inhibitors; D005591:Chemical Fractionation; D004347:Drug Interactions; D004357:Drug Synergism; D005260:Female; D018738:Hexamethonium / Q000494:pharmacology; D066298:In Vitro Techniques; D008012:Lidocaine / Q000494:pharmacology; D009388:Neostigmine / Q000494:pharmacology; D051381:Rats; D011976:Receptors, Muscarinic; D012604:Scorpion Venoms / Q000378:metabolism / Q000633:toxicity*; D012605:Scorpions; D012702:Serotonin Antagonists / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D014118:Toxins, Biological / Q000302:isolation & purification / Q000633:toxicity*; D014590:Uterine Contraction / Q000187:drug effects*
D011976:Receptors, Muscarinic; D012604:Scorpion Venoms; D012702:Serotonin Antagonists; D014118:Toxins, Biological; D000804:Angiotensin II; D009388:Neostigmine; D018738:Hexamethonium; D013779:Tetrodotoxin; D001285:Atropine; D008012:Lidocaine; D000109:Acetylcholine; D001920:Bradykinin