title: [Platelet collection by apheresis with reduced use of citrate: quantitative and qualitative evaluation of three protocols].
abstract: Three protocols of platelet collection by apheresis on Cobe Spectra with different citrate concentrations were compared. First we determined until what level we could diminish the ratio anticoagulant/whole blood flow to enhance the efficiency without clotting in the cell separator by platelet aggregates. We found that protocol 2 had a better platelet collection efficiency than the standard protocol 1: respectively 59% versus 52%. Protocol 3 had even a better efficiency but, due to the risk of clotting (5% of the procedures) we rejected it for routine use. We compared in vitro platelet functions of protocols 1 and 2 by aggregometry in vitro. The results with the different aggregating agents indicated that the less citrated environment kept the platelets more functional in vitro. A disadvantage of protocol 2 compared to the standard protocol is the more important number of platelet concentrates that contain more than 10(6) leucocytes.
authors.affiliation: Poste d'Hémobiologie-Transfusion de l'Hôtel-Dieu, Secteur d'Hémobiologie-Transfusion, Paris Centre.
authors.forename: J
authors.initials: J
authors.lastname: Moh-Klaren
authors.forename: N
authors.initials: N
authors.lastname: Berriot
authors.forename: C
authors.initials: C
authors.lastname: Lecrubier
authors.forename: M
authors.initials: M
authors.lastname: Samama
authors.forename: G
authors.initials: G
authors.lastname: Andreu
mesh_terms: D000328:Adult; D002951:Citrates; D019343:Citric Acid; D002985:Clinical Protocols; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D010974:Platelet Aggregation / Q000187:drug effects; D010983:Plateletpheresis / Q000379:methods*; D013223:Statistics as Topic
chemical_list: D002951:Citrates; D019343:Citric Acid
vernacular_title: Prélèvement de plaquettes par aphérèse avec réduction de l'utilisation de citrate: évaluation quantitative et qualitative de trois protocoles.