Cloning and functional expression of a human eosinophil CC chemokine receptor.
Eosinophils undergo chemotaxis, degranulate, and exhibit [C2+]i changes in response to the human CC chemokines macrophage inflammatory protein (MIP)-1 alpha, regulated on activation, normal T expressed and secreted (RANTES), and monocyte chemoattractant protein-3 (MCP-3), but the receptors involved have not been defined. We have isolated a human cDNA encoding the first eosinophil-selective chemokine receptor, designated CC chemokine receptor 3 (CC CKR3). CC CKR3 is a seven-transmembrane domain G protein-coupled receptor most closely related to the previously reported monocyte- and neutrophil-selective receptor CC CKR1 (also known as the MIP-1 alpha/RANTES receptor). When [Ca2+]i changes were monitored in stably transfected human embryonic kidney 293 cells, MIP-1 alpha and RANTES were both potent agonists for CC CKR3 and CC CKR1. However, MIP-1 beta was also an agonist for CC CKR3 but not CC CKR1; MCP-3 was an agonist for CC CKR1 but not CC CKR3. CC CKR3 may be one of the host factors responsible for selective recruitment of eosinophils to sites of inflammation.
Combadiere
C
C
Laboratory of Host Defenses, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA.
Ahuja
S K
SK
Murphy
P M
PM
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004804:Eosinophils / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D019713:Receptors, CCR5; D019707:Receptors, Chemokine; D011971:Receptors, Immunologic / Q000235:genetics* / Q000502:physiology
D012333:RNA, Messenger; D019713:Receptors, CCR5; D019707:Receptors, Chemokine; D011971:Receptors, Immunologic; C080633:macrophage inflammatory protein 1alpha receptor