Sequences in the human immunodeficiency virus type 1 U3 region required for in vivo and in vitro integration.
A series of mutants with alterations in the U3 region of the human immunodeficiency virus type 1 long terminal repeat were made, and the effects of these mutations were evaluated both in vitro and in vivo. When the subterminal 6 to 8 nucleotides of the U3 long terminal repeat were mutated, the resulting provirus was unable to efficiently replicate in vivo, and a mutant oligonucleotide which mimicked the mutation could not be efficiently cleaved but could be joined to target DNA by wild-type recombinant integrase protein in vitro. These results suggest that this region is important in the specific recognition of the viral DNA by the integrase protein.
Reicin
A S
AS
Department of Medicine, Columbia College of Physicians and Surgeons, New York, New York 10032, USA.
Kalpana
G
G
Paik
S
S
Marmon
S
S
Goff
S
S
D001483:Base Sequence; D002460:Cell Line; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006801:Humans; D019426:Integrases; D007700:Kinetics; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D011533:Proviruses / Q000235:genetics / Q000502:physiology; D011994:Recombinant Proteins / Q000378:metabolism; D015183:Restriction Mapping; D013379:Substrate Specificity; D014162:Transfection; D016662:Virus Integration; D014779:Virus Replication
D004279:DNA, Viral; D011994:Recombinant Proteins; D004254:DNA Nucleotidyltransferases; D019426:Integrases