Evaluation of phagocytic capacity with a modified flow cytometry technique.
Phagocytic capacity was determined in human polymorphonuclear leukocytes with a modified flow cytometry technique. The advantages of this method include simplified sample preparation--the assay is done using whole blood--and quantification of phagocytosis with a phagocytic index obtained by comparison with controls, which obviates errors arising from non-specific adherence.
Santos
J L
JL
Hospital Universitario de Granada, Departamento de Bioquímica y Biología Molecular, Universidad de Granada, Spain.
Montes
M J
MJ
Gutiérrez
F
F
Ruiz
C
C
D000925:Anticoagulants / Q000494:pharmacology; D016477:Artifacts; D004492:Edetic Acid / Q000494:pharmacology; D005434:Flow Cytometry / Q000379:methods*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007840:Latex; D007958:Leukocyte Count; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008863:Microspheres; D009504:Neutrophils / Q000502:physiology* / Q000648:ultrastructure; D010587:Phagocytosis / Q000187:drug effects
D000925:Anticoagulants; D007840:Latex; D006493:Heparin; D004492:Edetic Acid