Enhancement of calcium transport in the Caco-2 cell monolayer model.
The overall objective of this research was to identify enhancers of calcium transport using an in-vitro Caco-2 cell monolayer model. The enhancers studied were medium-chain triglycerides (MCT) and acylcarnitines (AC). The extent of cell damage associated with the use of these enhancers was determined by monitoring the release of cellular lactate dehydrogenase (LDH). The effect of chain-length and concentration dependence of these agents on enhancement were also determined. The effects of ACs were found to be superior to those of MCTs. However, the ACs elicited a greater release of LDH than the MCTs. The possible mechanisms of enhancer-mediated increase in calcium transport and the potential significance of this study with regard to the prevention of osteoporosis are discussed.
Surendran
N
N
Department of Pharmacology and Toxicology, University of Arizona, Tucson 85721, USA.
Nguyen
L D
LD
Giuliano
A R
AR
Blanchard
J
J
D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D002331:Carnitine / Q000494:pharmacology; D002460:Cell Line; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D014280:Triglycerides / Q000494:pharmacology
D014280:Triglycerides; D007770:L-Lactate Dehydrogenase; D002331:Carnitine; D002118:Calcium