title: Complex formation in yeast double-strand break repair: participation of Rad51, Rad52, Rad55, and Rad57 proteins.
abstract: The repair of DNA double-strand breaks in Saccharomyces cerevisiae requires genes of the RAD52 epistasis group, of which RAD55 and RAD57 are members. Here, we show that the x-ray sensitivity of rad55 and rad57 mutant strains is suppressible by overexpression of RAD51 or RAD52. Virtually complete suppression is provided by the simultaneous overexpression of RAD51 and RAD52. This suppression occurs at 23 degrees C, where these mutants are more sensitive to x-rays, as well as at 30 degrees C and 36 degrees C. In addition, a recombination defect of rad55 and rad57 mutants is similarly suppressed. Direct in vivo interactions between the Rad51 and Rad55 proteins, and between Rad55 and Rad57, have also been identified by using the two-hybrid system. These results indicate that these four proteins constitute part of a complex, a "recombinosome," to effect the recombinational repair of double-strand breaks.
authors.affiliation: Department of Biochemistry, Stanford University School of Medicine, Stanford University, CA 94305, USA.
authors.forename: S L
authors.initials: SL
authors.lastname: Hays
authors.forename: A A
authors.initials: AA
authors.lastname: Firmenich
authors.forename: P
authors.initials: P
authors.lastname: Berg
mesh_terms: D000251:Adenosine Triphosphatases; D004260:DNA Repair; D045643:DNA Repair Enzymes; D004268:DNA-Binding Proteins / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D005656:Fungal Proteins / Q000378:metabolism*; D005785:Gene Conversion; D046911:Macromolecular Substances; D008957:Models, Genetic; D051135:Rad51 Recombinase; D051721:Rad52 DNA Repair and Recombination Protein; D011836:Radiation Tolerance; D011994:Recombinant Proteins / Q000378:metabolism; D011995:Recombination, Genetic; D051716:Replication Protein A; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000528:radiation effects; D029701:Saccharomyces cerevisiae Proteins; D014965:X-Rays / Q000009:adverse effects
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D046911:Macromolecular Substances; C497197:RAD52 protein, S cerevisiae; C087191:RAD55 protein, S cerevisiae; D051721:Rad52 DNA Repair and Recombination Protein; D011994:Recombinant Proteins; D051716:Replication Protein A; D029701:Saccharomyces cerevisiae Proteins; C495151:RAD51 protein, S cerevisiae; D051135:Rad51 Recombinase; C037725:SCEI protein, S cerevisiae; D015252:Deoxyribonucleases, Type II Site-Specific; D000251:Adenosine Triphosphatases; C071451:RAD57 protein, S cerevisiae; D045643:DNA Repair Enzymes