Fluorescence of mast cell granules in paraffin sections and cell smears induced by an N-quaternary oxazole scintillator.
The N-quaternized derivative of dimethyl-POPOP (termed Q4) induces a bluish-green fluorescent reaction in mast cell granules from paraffin sections and cell smears, in addition to a previously described bluish-white fluorescent reaction in chromatin DNA. The chromatin reaction was abolished by staining the samples either with Mayer's Haematoxylin before Q4 treatment or by Q4 treatment at pH 1.5. The reaction in mast cell granules was absent after substrate methylation. The staining sequence Haematoxylin-Eosin-Q4 also worked well in paraffin sections, allowing the observation of the current histological image under bright-field illumination as well as double-colour emission under fluorescence microscopy. The sequence is proposed as a new diagnostic procedure for demonstrating mast cell granules.
Espada
J
J
Department of Pathology, La Paz Hospital, Faculty of Medicine, Autonomous University of Madrid, Spain.
Bertolesi
G E
GE
Trigoso
C I
CI
Gamallo
C
C
Stockert
J C
JC
D000818:Animals; D001940:Breast / Q000166:cytology / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000648:ultrastructure*; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D010080:Oxazoles; D016612:Paraffin Embedding; D012867:Skin / Q000166:cytology / Q000378:metabolism
D010080:Oxazoles; C007478:dimethyl-POPOP