title: Molecular analysis of Japanese isolates of Verticillium dahliae and V. albo-atrum.
abstract: Sixteen isolates of different pathogenicity groups of the plant pathogen Verticillium dahliae and four isolates of V. albo-atrum from Japan were analysed by means of an RAPD (random amplified polymorphic DNA) method using a PCR (polymerase chain reaction). Verticillium dahliae and V. albo-atrum could be distinguished by RAPD analysis. Four pathogenicity groups of V. dahliae could also be classified to a certain extent by this method. Similarities and differences in banding patterns obtained by RAPD may be a useful molecular tool in phylogenetic studies of the pathogenicity groups.
authors.affiliation: Department of Agro-environmental Science, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Koike
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Nagao
authors.forename: S
authors.initials: S
authors.lastname: Ohshima
mesh_terms: D001483:Base Sequence; D004271:DNA, Fungal / Q000235:genetics; D007564:Japan; D000455:Medicago sativa / Q000382:microbiology; D003904:Mitosporic Fungi / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D014675:Vegetables / Q000382:microbiology
chemical_list: D004271:DNA, Fungal