Identification of a novel mitogen-inducible gene (mig-6): regulation during G1 progression and differentiation.
We have identified a novel human gene (mig-6) that is rapidly induced upon mitogenic stimulation of quiescent fibroblasts. Serum induction is partially inhibited by protein synthesis inhibitors, indicating that mig-6 shares characteristics of both primary and secondary response genes. In contrast to most other mitogen-responsive genes, mig-6 mRNA expression is also regulated during normal cell cycle progression, showing a clear peak around mid-G1. Consistent with the regulation of mig-6 expression during the cell cycle, terminal differentiation of HL-60 cells to either granulocytic or macrophage-like cells also leads to clear changes in the levels of mig-6 mRNA. These observations suggest that the mig-6 gene represents a useful tool for the analysis of cell cycle progression and perhaps terminal differentiation. As a first step toward a functional characterization we show that the Mig-6 polypeptide is located in the cytoplasm.
Wick
M
M
Institut für Molekularbiologie und Tumorforschung (IMT), Philipps-Universität Marburg, Germany.
Bürger
C
C
Funk
M
M
Müller
R
R
D000595:Amino Acid Sequence; D001483:Base Sequence; D002451:Cell Compartmentation; D002453:Cell Cycle / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002461:Cell Line, Transformed; D003001:Cloning, Molecular; D016193:G1 Phase / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic; D005796:Genes / Q000235:genetics*; D006801:Humans; D008934:Mitogens / Q000494:pharmacology; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D008934:Mitogens