Restoration of myo-inositol uptake by eicosapentaenoic acid in human skin fibroblasts cultured in high-glucose medium.
The effects of elevated glucose and eicosapentaenoic acid (EPA, C20:5 omega 3) on myo-inositol uptake in human skin fibroblasts (HSF) were evaluated. Myo-inositol incorporation into HSF was dependent on an active transport system via Na(+)-K+ ATPase activity based on the results with Na+ deprivation and ouabain (5 mM). Although glucose (27.5, 55 mM) inhibited 2-[3H] myo-inositol uptake, the addition of EPA (3 x 10(-4) M) prevented glucose-mediated inhibition. Since EPA decreased glucose-mediated inhibition of myo-inositol uptake, this agent might ameliorate some of the devastating functions associated with diabetes.
Okuda
Y
Y
Department of Internal Medicine, University of Tsukuba, Japan.
Sawada
T
T
Mizutani
M
M
Sone
H
H
Suzuki
S
S
Kawakami
Y
Y
Soma
M
M
Suzuki
M
M
Yaoita
H
H
Yamashita
K
K
D002478:Cells, Cultured; D003470:Culture Media; D015118:Eicosapentaenoic Acid / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007294:Inositol / Q000378:metabolism*; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D003470:Culture Media; D007294:Inositol; D015118:Eicosapentaenoic Acid; D005947:Glucose