title: A comparison of the leech Theromyzon tessulatum angiotensin I-like molecule with forms of vertebrate angiotensinogens: a hormonal system conserved in the course of evolution.
abstract: After five steps of purification including gel permeation, anti-angiotensin I affinity column chromatography followed by reverse-phase HPLC, a peptide immunoreactive to two different antisera (anti-angiotensin II and anti-angiotensin I) was purified to homogeneity from extracts of the leech Theromyzon tessulatum. The first 14 amino acid residues of the purified peptide (DRVYIHPFHLLXWG) established by automated Edman degradation, reveal the existence in leeches of an angiotensin I-like molecule close to human angiotensin I. The sequence of the purified peptide presents 78.5% of homology with the N-terminal part of human angiotensinogen. Moreover, in its sequence, this peptide presents the cleavage sites of vertebrate angiotensin metabolic enzymes, i.e. the renin and the angiotensin-converting enzyme. This finding constitutes the first biochemical characterization of an angiotensin I in Invertebrates. It also reflects the high conservation of angiotensins in the course of evolution, suggesting a fundamental role of this family in fluid homeostasis.
authors.affiliation: Laboratoire de Phylogénie moléculaire des Annélides, ER 87 CNRS, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
authors.forename: V
authors.initials: V
authors.lastname: Laurent
authors.forename: P
authors.initials: P
authors.lastname: Bulet
authors.forename: M
authors.initials: M
authors.lastname: Salzet
mesh_terms: D000595:Amino Acid Sequence; D000803:Angiotensin I / Q000737:chemistry*; D000808:Angiotensinogen / Q000737:chemistry; D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D002851:Chromatography, High Pressure Liquid; D007106:Immune Sera / Q000276:immunology; D007150:Immunohistochemistry; D007700:Kinetics; D007865:Leeches / Q000737:chemistry*; D008969:Molecular Sequence Data; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry
chemical_list: D007106:Immune Sera; D000808:Angiotensinogen; D000803:Angiotensin I; D007703:Peptidyl-Dipeptidase A