Diabetes impairs DRG neuronal attachment to extracellular matrix proteins in vitro.
Attachments of dorsal root ganglion (DRG) neurons from streptozotocin (STZ)-induced diabetic and normal C57BL mice to the following substrates were evaluated in vitro: a) poly L-lysine (PL), b) PL + type I collagen (CL-I), c) PL + type IV collagen (CL-IV), d) PL + laminin (LM) and e) PL + fibronectin (FN). After 6 h in culture, there was no significant difference in the average ratio of cells adhesive to PL between the diabetic (74.9%) and normal group of mice (75.6%). In the normal group, the addition of extracellular matrix (ECM) proteins such as CL-I, CL-IV, LM and FN to PL increased the ratios of cell attachment from 75.6% to nearly 90%. In the diabetic group, however, none of these proteins improved the attachment (the ratio changed from 74.9% to nearly 70%). Survival and neurite extension of attached cells after 48 h in culture were not different between the two groups. These results suggest that the cell-surface receptors, which enable DRG neurons to bind to the extracellular matrix proteins, are impaired by diabetes, resulting in being one of the causes of diabetic neuropathy.
Sango
K
K
Department of Physiology, School of Medicine, Yokohama City University, Japan.
Horie
H
H
Okamura
A
A
Inoue
S
S
Takenaka
T
T
D000818:Animals; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000166:cytology / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016501:Neurites / Q000187:drug effects; D009474:Neurons / Q000502:physiology*; D011485:Protein Binding / Q000502:physiology
D016326:Extracellular Matrix Proteins