Cell cytotoxicity of sodium nitrite, sodium nitroprusside and Roussin's black salt against Trichomonas vaginalis.
We have investigated the action of sodium nitrite and other nitrosyl complexes, such as sodium nitroprusside and Roussin's black salt, on the growth of metronidazole-sensitive and resistant strains of Trichomonas vaginalis and their hydrogenosomal enzymes. All three chemicals inhibited the growth of T. vaginalis: sodium nitrite at 8 mM, sodium nitroprusside at 1.2 mM and Roussin's black salt at 0.2 mM. Metronidazole-sensitive (KT9) and resistant (CDC85) isolates showed similar cytotoxicity against these molecules. Specific activities of pyruvate:ferredoxin oxidoreductase and hydrogenase and oxygen uptake rates were decreased in the T. vaginalis isolate treated with sodium nitrite and sodium nitroprusside. However, Roussin's black salt increased the specific activity of pyruvate:ferredoxin oxidoreductase or hydrogenase in CDC85 or KT9 cells and increased the oxygen uptake rate in the KT9 isolate.
Ryu
J S
JS
Department of Parasitology, College of Medicine, Hanyang University, Seoul, South Korea.
Lloyd
D
D
D000818:Animals; D007502:Iron Chelating Agents / Q000494:pharmacology*; D058085:Iron Compounds; D009599:Nitroprusside / Q000494:pharmacology*; D009603:Nitroso Compounds; D012977:Sodium Nitrite / Q000494:pharmacology*; D014246:Trichomonas vaginalis / Q000187:drug effects*
D007502:Iron Chelating Agents; D058085:Iron Compounds; D009603:Nitroso Compounds; D009599:Nitroprusside; C078952:Roussin's Black Salt; D012977:Sodium Nitrite