title: beta-Adrenergic stimulation induces acetylcholine to activate ATP-sensitive K+ current in cat atrial myocytes.
abstract: Our previous work on atrial myocytes suggested that the effect of acetylcholine (ACh) to increase K+ conductance can be potentiated by prior loading of the sarcoplasmic reticulum (SR) with Ca2+. The present study, therefore, sought to determine whether prior exposure to isoproterenol (ISO) potentiates ACh-induced increases in K+ conductance and the underlying mechanisms. A nystatin-perforated patch whole-cell configuration was used to record from cat atrial myocytes. Voltage-clamp ramps (40 mV/s) were used to assess total membrane conductance. The experimental protocol consisted of two consecutive 30-second ACh exposures (ACh1 and ACh2) separated by a 6-minute recovery period in ACh-free solution. In general, experimental interventions, such as exposure to ISO, were imposed during the period between ACh1 and ACh2 to determine their effects on the response to ACh2 in relation to ACh1. Under control conditions, K+ conductances induced by ACh1 and ACh2 were not different from one another with or without activation of L-type Ca2+ current (ICa,L) during the recovery period. When 1 mumol/L ISO plus ICa,L activation was imposed during the recovery period, ACh2 induced a significantly larger increase in K+ conductance than ACh1. The ACh2-induced K+ current potentiated by ISO was time independent and selectively blocked by 10 mumol/L glibenclamide and therefore identified as ATP-sensitive K+ current (IK,ATP). The effect of ISO to induce ACh2-activated IK,ATP was mimicked by 1 mumol/L forskolin or 200 mumol/L 8-(4-chlorophenylthio)-cAMP, but not by 0.5 mumol/L BAY K 8644, and was selectively abolished by (1) 5 mumol/L thapsigargin or 1 mumol/L ryanodine, agents that prevent accumulation of SR Ca2+, (2) inhibition of L-type Ca2+ current (ICa,L) by 1 mumol/L nisoldipine or zero external Ca2+, (3) 50 mumol/L Rp-cAMPs, an inhibitor of cAMP-dependent protein kinase A, or (4) 2 mumol/L propranolol. Atropine (1 mumol/L) abolished all ACh-induced currents. Moreover, ACh2-activated IK,ATP was selectively blocked by 0.2 mumol/L pirenzepine, an M1 muscarinic receptor antagonist, or 0.1 mumol/L calphostin C, a selective inhibitor of protein kinase C. AFDX116 (100 mumol/L), an M2 muscarinic receptor antagonist, blocked the conventional ACh-activated K+ current and revealed ACh2-activated IK,ATP. These results indicate that prior exposure to ISO potentiates ACh-induced increases in K+ current via ACh-activated IK,ATP.(ABSTRACT TRUNCATED AT 400 WORDS)
authors.affiliation: Department of Physiology, Loyola University of Chicago, Stritch School of Medicine, Maywood, Ill., USA.
authors.forename: Y G
authors.initials: YG
authors.lastname: Wang
authors.forename: S L
authors.initials: SL
authors.lastname: Lipsius
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002415:Cats; D000242:Cyclic AMP / Q000502:physiology; D005260:Female; D005905:Glyburide / Q000494:pharmacology; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D015221:Potassium Channels / Q000187:drug effects*; D011493:Protein Kinase C / Q000502:physiology; D011943:Receptors, Adrenergic, beta / Q000187:drug effects*; D011976:Receptors, Muscarinic / Q000145:classification; D012519:Sarcoplasmic Reticulum / Q000378:metabolism
chemical_list: D015221:Potassium Channels; D011943:Receptors, Adrenergic, beta; D011976:Receptors, Muscarinic; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D011493:Protein Kinase C; D007545:Isoproterenol; D000109:Acetylcholine; D005905:Glyburide; D002118:Calcium