Meiotic pairing in wheat-rye derivatives detected by genomic in situ hybridization and C-banding--A comparative analysis.
Meiosis of triticalextetraploid rye hybrids (genome constitution ABRRR) was analysed by genomic in situ hybridization (GISH) and C-banding. The results obtained reveal a considerable difference between these techniques with regard to their efficiency in detecting any type of pairing, either homologous or homoeologous. Thus the percentage of pollen mother cells containing wheat/rye homoeologous associations determined by C-banding and GISH was 2.5 and 9.2, respectively. Such a discrepancy can be ascribed to a certain proportion of wheat/rye associations not being identified by C-banding. The potential and limitations of the two techniques for meiotic analysis are discussed.
Fernández-Calvín
B
B
Unidad de Genética, E.T.S.1. Agrónomos, Universidad Politécnica de Madrid, Spain.
Benavente
E
E
Orellana
J
J
D002678:Chimera; D002871:Chromosome Banding; D002875:Chromosomes / Q000648:ultrastructure; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D008540:Meiosis / Q000235:genetics*; D008856:Microscopy, Fluorescence; D008938:Mitosis / Q000235:genetics; D012434:Secale / Q000235:genetics*; D014908:Triticum / Q000235:genetics*