title: Enhanced expression of MHC class I molecules on cultured human thyroid follicular cells infected with reovirus through induction of type 1 interferons.
abstract: Certain viruses are known to modulate the cellular expression of MHC molecules. We have investigated whether reovirus types 1 or 3 can alter the normal MHC molecule expression on cultured human thyroid follicular cells (TFC). Primary TFC cultures were established from eight human thyroid donors and MHC class I and II expression was assessed by indirect immunofluorescence microscopy. Both types of reovirus enhanced MHC class I expression on TFC from all thyroid donors. Class II MHC protein was strongly induced by type 1 reovirus on TFC from one donor, while weak induction of expression, by either reo-1 or reo-3 virus, was noted on the TFC of five other donors. Studies on the mechanism(s) of MHC class I hyperexpression showed that mouse MoAb against the type 3 reovirus haemagglutinin (anti-HA3) reduced the ability of the virus to induce hyperexpression of class I MHC molecules on TFC. However, supernatant harvested from type 3 reovirus-infected TFC cultures maintained its ability to enhance class I expression after incubation with anti-HA3. Moreover, adding rabbit anti-sera to interferon-alpha (IFN-alpha) or IFN-beta inhibited the increased class I MHC expression on TFC by both types of reovirus. These data suggest that reoviruses (types 1 and 3) can enhance MHC class I on cultured TFC. The mechanism of MHC class I enhancement is most probably through the release of IFN-alpha and IFN-beta.
authors.affiliation: Department of Clinical Laboratory Sciences, University Hospital, Queen's Medical Centre, Nottingham, UK.
authors.forename: M S
authors.initials: MS
authors.lastname: Atta
authors.forename: W L
authors.initials: WL
authors.lastname: Irving
authors.forename: R J
authors.initials: RJ
authors.lastname: Powell
authors.forename: I
authors.initials: I
authors.lastname: Todd
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D006384:Hemagglutination / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000187:drug effects; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis / Q000187:drug effects; D006801:Humans; D007370:Interferon Type I / Q000276:immunology / Q000494:pharmacology*; D009500:Neutralization Tests / Q000379:methods; D012088:Reoviridae Infections / Q000276:immunology / Q000378:metabolism*; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism* / Q000821:virology*
chemical_list: D000911:Antibodies, Monoclonal; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007370:Interferon Type I