Patterns of prostaglandin E2 and leukotriene B4 production in inflammation induced by peptidoglycan-polysaccharide polymers of bacterial cell walls.
The changing levels of prostaglandin E2 (PGE2) and leukotriene B4 (LTB4) were compared over a period of 1 h to 15 days in two kinds of inflammatory responses induced in the rat air pouch by chemically similar polymers of peptidoglycan-polysaccharide (PG-PS) isolated from cell walls of group A streptococci. The smaller polymers (100 s, average mol. wt 5.3 x 10(6)) induced a more severe acute response over the first 24 h, whereas, from 3 to 15 days the larger polymers (10 p, average mol. wt 5 x 10(8)) induced greater chronic inflammation, as measured by pouch fluid volume, number of infiltrating cells and weight of granulation tissue. The most prominent difference between the two kinds of responses in the pattern of PGE2 and LTB4 were: (a) A shift between 1 to 6 h to much higher production of PGE2 in the exudate induced by large polymers. During this time the level of LTB4 also increased, but continued to be higher in the exudate induced by small polymers. (b) The sustained production of relatively high levels of LTB4 and PGE2 in the large polymer exudate at 15 days. Changes such as these indicate that this model is useful for analyzing mediators which regulate the evolution of acute into chronic inflammation.
Yoshino
S
S
Department of Microbiology, Saga Medical School, Japan.
D000208:Acute Disease; D000818:Animals; D002473:Cell Wall; D002908:Chronic Disease; D015232:Dinoprostone / Q000096:biosynthesis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006097:Granulation Tissue / Q000187:drug effects / Q000378:metabolism; D006099:Granuloma / Q000139:chemically induced / Q000378:metabolism; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D007975:Leukotriene B4 / Q000096:biosynthesis*; D008970:Molecular Weight; D010457:Peptidoglycan / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew; D013297:Streptococcus pyogenes / Q000737:chemistry
D010457:Peptidoglycan; D007975:Leukotriene B4; D015232:Dinoprostone