Transfection of Sendai virus F gene cDNA with mutations at its cleavage site and HN gene cDNA into COS cells induces cell fusion.
In contrast to the wild type Sendai virus fusion protein (F), a mutated F to possess a cleavage site similar to that of virulent Newcastle disease virus F, could be cleaved by proteases present in COS cells. When mutated F and hemagglutinin-neuraminidase (HN) were coexpressed at the cell surface, syncytium formation was observed.
Taira
H
H
Department of Bioscience and Technology, Faculty of Agriculture, Iwate University, Morioka, Japan.
Sato
T
T
Segawa
H
H
Chiba
M
M
Katsumata
T
T
Iwasaki
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D004279:DNA, Viral; D015726:Giant Cells / Q000821:virology; D015707:HN Protein / Q000235:genetics / Q000502:physiology*; D006868:Hydrolysis; D008561:Membrane Fusion; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010222:Parainfluenza Virus 1, Human / Q000235:genetics / Q000502:physiology*; D014162:Transfection; D014760:Viral Fusion Proteins / Q000235:genetics / Q000502:physiology*
D004279:DNA, Viral; D015707:HN Protein; D014760:Viral Fusion Proteins