Viscum album agglutinin-induced aggregation of blood cells and the lectin effects on neutrophil function.
Extracts from mistletoe enjoy a large popularity in central Europe as an unconventional treatment modality for cancer, warranting scientific efforts with defined components to delineate any potential benefit. The galactose-specific lectin from Viscum album (VAA), known to exhibit immunomodulatory and ensuing antitumoral capacities in animal model systems, was shown to aggregate human blood cells in the following order: neutrophils, mononuclear cells--thrombocytes and erythrocytes. To contribute to the analysis of lectin effects on individual aspects of the host defence system, two parameters of neutrophils were quantitatively assessed, namely the aggregating activity of VAA as a measure of strength of interaction with cell surface ligands and the effect of lectin on oxidative metabolism (H2O2 release) of these cells. It was found that whole lectin and its carbohydrate-binding B-subunit possessed the capacity to induce cell aggregation and H2O2 release, which were blocked by D-galactose and lactose. Both effects displayed similar dependence on the lectin concentration in the range 0.1-25 micrograms/ml. The toxic A-subunit displayed detectable activity only in high doses (50 micrograms/ml) while the bovine heart galaptin (14 kDa; galectin-1) failed to affect neutrophils. The role of oxidative metabolism in regulation of neutrophil aggregation induced by VAA was studied using metabolic inhibitors and controlled heating at 46 degrees C leading to inhibition of plasma membrane NADPH-oxidase system. Trifluoperazine and menadione inhibited the neutrophil aggregation in a dose-dependent manner in comparison with such inhibitors as amiloride and theophylline.(ABSTRACT TRUNCATED AT 250 WORDS)
Timoshenko
A V
AV
Department of Biophysics, Belarusian State University, Minsk.
Cherenkevich
S N
SN
Gabius
H J
HJ
D001773:Blood Cells / Q000378:metabolism*; D002449:Cell Aggregation / Q000187:drug effects; D017666:Deuterium Oxide / Q000097:blood; D005060:Europe; D006358:Hot Temperature; D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology*; D014784:Mistletoe / Q000737:chemistry*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D037121:Plant Lectins; D010946:Plants, Medicinal; D016897:Respiratory Burst / Q000187:drug effects
D037102:Lectins; D037121:Plant Lectins; D017666:Deuterium Oxide