title: Peptide motif of a cattle MHC class I molecule.
abstract: A consensus motif for a bovine major histocompatibility complex (MHC) class I molecule, A20, was derived from parainfluenza type-3 (PI-3) virus-infected muscle-derived fibroblast cells and peripheral blood leukocytes by extraction of the naturally processed peptides from MHC class I molecules by treatment with TFA and peptide sequencing of the complex mixture. The results showed that the majority of peptides were 9 amino acids long with position 2 occupied by lysine and position 9 occupied by arginine. The arginine at position 9 suggests that cattle, like humans, but unlike the mouse have permissive TAP transporter molecules accepting peptides with positively charged amino acids at their C-terminus. This is the first report of a MHC ligand motif in cattle.
authors.affiliation: DKFZ, Department of Tumour Virus Immunology, Heidelberg I, Germany.
authors.forename: A I
authors.initials: AI
authors.lastname: Bamford
authors.forename: A
authors.initials: A
authors.lastname: Douglas
authors.forename: T
authors.initials: T
authors.lastname: Friede
authors.forename: S
authors.initials: S
authors.lastname: Stevanovic
authors.forename: H G
authors.initials: HG
authors.lastname: Rammensee
authors.forename: B M
authors.initials: BM
authors.lastname: Adair
mesh_terms: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D001120:Arginine; D002417:Cattle / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D016384:Consensus Sequence; D005347:Fibroblasts / Q000276:immunology / Q000821:virology; D015395:Histocompatibility Antigens Class I / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D006825:Hybridomas / Q000276:immunology; D007962:Leukocytes / Q000276:immunology / Q000821:virology; D051379:Mice; D008969:Molecular Sequence Data; D010224:Parainfluenza Virus 3, Human / Q000276:immunology*; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology; D011485:Protein Binding; D016415:Sequence Alignment; D013045:Species Specificity; D014764:Viral Proteins / Q000737:chemistry* / Q000276:immunology
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D000956:Antigens, Viral; D015395:Histocompatibility Antigens Class I; D010446:Peptide Fragments; C073119:TAP1 protein, human; C486171:Tap1 protein, mouse; C489516:Tap2 protein, mouse; D014764:Viral Proteins; C071692:TAP2 protein, human; D001120:Arginine