Antigens identified by monoclonal antibodies in tissue sections of Boophilus microplus.
A panel of monoclonal antibodies (mAbs) raised against midgut antigens of Boophilus microplus were used to probe various stages and organs of the tick. One of the monoclonal antibodies in this panel (QU13) has previously been shown to recognize protective antigens. Of the 18 mAbs tested, all except two (QU5 and QU12) reacted with sections of adult midgut and Malpighian tubules using an avidin-biotin alkaline phosphatase method for immunostaining. MAbs QU1, QU2, QU3, QU4, QU12, QU13, and QU18 reacted specifically with the lumenal surfaces of type III acini of the salivary gland. These seven mAbs also stained the midgut in larval sections indicating that the antigens recognized were not stage specific. However, none of the seven mAbs tested recognised antigens in either the adult ovary or the developing egg. Antigens which were immunogold labelled by mAbs QU1, QU4, QU11, QU13, and QU15 in electron microscopy were located either on or near the surface of the microvilli of digestive cells from the midgut of the adult tick. We conclude that common antigens are present on the lumenal surfaces of the adult midgut, type III acini of the salivary gland, and the Malpighian tubules and that these antigens are also located in the larval gut.
Lee
R P
RP
Centre for Animal Biotechnology, University of Melbourne, Parkville, Victoria, Australia.
Opdebeeck
J P
JP
D000818:Animals; D000911:Antibodies, Monoclonal; D000941:Antigens / Q000032:analysis*; D002417:Cattle; D004064:Digestive System / Q000166:cytology* / Q000648:ultrastructure; D005260:Female; D007150:Immunohistochemistry; D007814:Larva; D008317:Malpighian Tubules / Q000166:cytology; D008854:Microscopy, Electron; D016253:Microscopy, Immunoelectron; D010053:Ovary / Q000166:cytology; D010063:Ovum / Q000166:cytology; D012469:Salivary Glands / Q000166:cytology*; D013987:Ticks / Q000166:cytology*
D000911:Antibodies, Monoclonal; D000941:Antigens