Hemolytic activity of the Pasteurella haemolytica leukotoxin.
A Pasteurella haemolytica mutant incapable of producing leukotoxin was created by allelic replacement. Concentrated culture supernatants from wild-type P. haemolytica, but not from the mutant, contained the 102-kDa leukotoxin protein and lysed bovine lymphoma cells and sheep erythrocytes. Wild-type P. haemolytica demonstrated the typical beta-hemolytic phenotype on sheep and rabbit blood agar, whereas the mutant did not.
Murphy
G L
GL
Department of Veterinary Pathology, College of Veterinary Medicine, Oklahoma State University, Stillwater 74078, USA.
Whitworth
L C
LC
Clinkenbeard
K D
KD
Clinkenbeard
P A
PA
D000818:Animals; D001427:Bacterial Toxins / Q000633:toxicity*; D004269:DNA, Bacterial / Q000235:genetics; D005098:Exotoxins / Q000633:toxicity*; D005798:Genes, Bacterial; D006460:Hemolysin Proteins; D066298:In Vitro Techniques; D016978:Mannheimia haemolytica / Q000472:pathogenicity*; D016296:Mutagenesis; D011817:Rabbits; D012756:Sheep
D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005098:Exotoxins; D006460:Hemolysin Proteins; C018579:leukotoxin