title: Proofreading in vivo. Editing of homocysteine by aminoacyl-tRNA synthetases in Escherichia coli.
abstract: Editing reactions are an essential part of biological information transfer processes that require high accuracy, such as replication, transcription, and translation. The editing in amino acid selection for protein synthesis by an aminoacyl-tRNA synthetase, the first proofreading process discovered in the flow of genetic information, prevents attachment of incorrect amino acids to tRNA. Of numerous editing reactions studied in vitro, only one, editing of homocysteine by methionyl-tRNA synthetase, has also been demonstrated in vivo. It is therefore unclear to what extent editing of errors is physiologically relevant. Here we show that isoleucyl- and leucyl-tRNA synthetases also edit homocysteine by cyclizing it to homocysteine thiolactone in the bacterium Escherichia coli. These and other data also suggest that metabolite compartmentation or channeling governs which synthetase participates in editing in bacterial cells.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry, New Jersey Medical School, Newark 07103, USA.
authors.forename: H
authors.initials: H
authors.lastname: Jakubowski
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000494:pharmacology; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D006710:Homocysteine / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids; D017393:RNA Editing
chemical_list: D000596:Amino Acids; D006710:Homocysteine; C007957:homocysteine thiolactone; D000604:Amino Acyl-tRNA Synthetases