Replication of measles virus in human monocytes and T cells.
Replication of measles virus (MV) in populations of peripheral blood mononuclear cells enriched for T cells and monocytes was studied using a temperature-sensitive mutant, MV ts38, and the parent counterpart, MV Lec. Stimulation of the cells was required for a full cycle of virus replication in both cell types. More infectious virus was released after stimulation from MV-infected populations enriched for T cells, T cell-enriched than from monocyte-enriched populations. However, similar amounts of viral mRNA, genomic RNA, and viral proteins of the expected size were found in both cell populations. The results indicate that MV-specific macromolecular synthesis is similar in both T cells and monocytes, but the assembly and (or) release of infectious virus is greatly reduced in monocytes as compared with T cells.
Vydelingum
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S
Department of Virology, University of Turku, Finland.
Suryanarayana
K
K
Marusyk
R G
RG
Salmi
A A
AA
D002455:Cell Division; D006801:Humans; D008459:Measles virus / Q000235:genetics / Q000502:physiology*; D009000:Monocytes / Q000166:cytology / Q000821:virology*; D009154:Mutation; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000166:cytology / Q000821:virology*; D013696:Temperature; D015678:Viral Structural Proteins / Q000096:biosynthesis; D014779:Virus Replication
D012333:RNA, Messenger; D012367:RNA, Viral; D015678:Viral Structural Proteins