Effects of acetaminophen on methemoglobin, superoxide dismutase and Na(+)-K+ ATPase activities of human erythrocytes.
It is established that acetaminophen exhibits oxidative behaviour. The effects of acetaminophen (0.3-14.5 microM) on methemoglobin levels, superoxide dismutase and Na(+)-K+ ATPase activities of normal and vitamin E or vitamin C pretreated erythrocytes were investigated. In acetaminophen incubated erythrocytes, methemoglobin concentration and superoxide dismutase activity were increased in a dose and incubation-time dependent manner, the activity of Na(+)-K+ ATPase was decreased by acetaminophen treatment. Vitamin E (1mg/dl of erythrocyte suspension) or vitamin C (1mg/dl of erythrocyte suspension) provided partial protection of hemoglobin, superoxide dismutase and Na(+)-K+ ATPase against acetaminophen action. Vitamin E was more effective than vitamin C.
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Cukurova University, Art and Science Faculty, Department of Chemistry, Adana, Turkiye.
D000082:Acetaminophen / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008706:Methemoglobin / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000097:blood*; D014807:Vitamin D / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology
D014807:Vitamin D; D014810:Vitamin E; D000082:Acetaminophen; D008706:Methemoglobin; D013482:Superoxide Dismutase; D000254:Sodium-Potassium-Exchanging ATPase