Programmed cell death of PC12 induced by adenovirus E1A.
PC12 rat pheochromocytoma cells undergo programmed cell death or apoptosis with DNA fragmentation when deprived of serum. Here, we isolated a subclone of PC12, PC12FrR, that was resistant to DNA fragmentation in a serum-free condition. PC12FrR grew slightly faster, and had cells that were slightly larger than parental PC12 cells. Adenovirus E1A gene-transfected PC12FrR cells grew much faster than did parental PC12FrR cells in the presence of serum. In a serum-deprived condition, E1A-transfected PC12FrR cells died with DNA fragmentation, as did PC12 cells under the same conditions. These results suggest that the target(s) of E1A gene products may be involved in the mechanism(s) that regulate growth and death of neuronal cells.
Sawada
M
M
Division of Cell Biology, Fujita Health University, Aichi, Japan.
Kondo
N
N
Marunouchi
T
T
D000256:Adenoviridae / Q000235:genetics / Q000502:physiology*; D000818:Animals; D017209:Apoptosis; D001769:Blood; D002455:Cell Division; D004249:DNA Damage; D015870:Gene Expression; D016716:PC12 Cells; D051381:Rats