Jun-B oncogene aberrations in cervical cancer cell lines.
We describe here structural and expression analysis of the jun-B oncogene in two cervical cancer cell lines. In the CC7T-a cell line, results from both Southern analysis and cDNA cloning studies revealed the existence of two structurally altered jun-B alleles besides the normal gene. One of the altered alleles was due to a type 16 human papillomavirus (HPV-16) integration event, whereas the other allele was a consequence of a chromosomal translocation involving chromosome 19 (jun-B) and an EST182 locus residing in chromosome 15. In the HeLa cell line, which contains integrated HPV-18, an apparent structural aberration, a 3-fold amplification and a 3-fold overexpression of the jun-B gene were observed. Our observations suggest that deregulation of the jun-B gene expression may have contributed to the transformation process in these two cervical cancer cell lines.
Choo
K B
KB
Department of Medical Research, Veterans General Hospital, Taipei, Taiwan.
Huang
C J
CJ
Chen
C M
CM
Han
C P
CP
Au
L C
LC
D000483:Alleles; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016758:Genes, jun / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014162:Transfection; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
D018076:DNA, Complementary; D004273:DNA, Neoplasm