title: Production and some characteristics of beta-glucosidase in Diaporthe (Phomopsis) helianthi.
abstract: Diaporthe (Phomopsis) helianthi Munt.-Cvet. et al. is an important phytopathogenic fungus which causes stem canker of sunflower. When grown in submerged cultures in the presence of milled wheat chaff as a carbon source this phytopathogen produced extracellular and intracellular beta-glucosidases. The optimum pH of these enzymes was 4.8. The temperature of crude intracellular beta-glucosidase activity was at 60 degrees C, whereas the optimum activity of crude extracellular beta-glucosidase was observed in a wide range of temperature between 40 and 70 degrees C. Although, extracellular and intracellular beta-glucosidase activities had identical pH and similar temperature optima, the thermal stability of the intracellular enzyme was significantly higher.
authors.affiliation: Faculty of Technology, University of Novi Sad, Yugoslavia.
authors.forename: D
authors.initials: D
authors.lastname: Pericin
authors.forename: M
authors.initials: M
authors.lastname: Jarak
mesh_terms: D005110:Extracellular Space / Q000201:enzymology; D005285:Fermentation; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D005658:Fungi / Q000201:enzymology* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007424:Intracellular Fluid / Q000201:enzymology; D010935:Plant Diseases / Q000382:microbiology; D010945:Plants, Edible / Q000382:microbiology; D013379:Substrate Specificity; D013696:Temperature; D001617:beta-Glucosidase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D001617:beta-Glucosidase