Endogenous interleukin 10 prevents apoptosis in macrophages during Salmonella infection.
To elucidate the biological roles of endogenous interleukin 10 (IL-10) in macrophage responses during bacterial infection, we examined in vitro effects of neutralizing IL-10 by anti-IL-10 monoclonal antibodies (mAb) on apoptosis of the peritoneal macrophages following Salmonella choleraesuis infection. Marked increments of TNF-alpha production were observed in the culture supernatant later than 6 h after in vitro culture with anti-IL-10 mAb. These macrophages succumbed to apoptosis at this stage accompanied by marked increment of IL-1 release, despite the expression of higher amount of endogenous heat shock protein 70, an inhibitor of TNF-alpha-mediated apoptosis. These results suggest that endogenous IL-10 plays an essential role in protection of Salmonella-infected macrophages from autocrine suicide caused by excessive production of TNF-alpha after killing of Salmonella.
Arai
T
T
Laboratory of Host Defense and Germfree Life, Nagoya University School of Medicine, Japan.
Hiromatsu
K
K
Nishimura
H
H
Kimura
Y
Y
Kobayashi
N
N
Ishida
H
H
Nimura
Y
Y
Yoshikai
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000502:physiology*; D017737:Macrophages, Peritoneal / Q000382:microbiology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D012481:Salmonella Infections, Animal / Q000473:pathology*
D000911:Antibodies, Monoclonal; D018840:HSP70 Heat-Shock Proteins; D016753:Interleukin-10; D009569:Nitric Oxide