DNase I interaction on muscle Z-line.
The effect of deoxyribonuclease I on muscle Z-line structures was re-examined. Under conditions of deoxyribonuclease I activation (presence of the divalent cation Ca2+ and Mg2+), a deoxyribonuclease I preparation did not affect Z-line structure if phenylmethylsulfonylfluoride, an inhibitor of serine proteases, was also present. In the absence of protease inhibitor, both Z-lines and M-lines were digested, even in the presence of EDTA and EGTA as inhibitors of deoxyribonuclease I. These electron microscopic observations were consistent with the following results from sodium dodecyl sulphate gel electrophoresis: when the protease was inhibited but deoxyribonuclease I was activated, myofibrillar proteins remained essentially intact. However, degradation of proteins in both rabbit psoas and chicken pectoralis myofibrils was observed in the presence of deoxyribonuclease I inhibitors when the protease inhibitor was absent. Our data strongly suggest that the interaction of deoxyribonuclease I with Z-line proteins previously reported is most likely due to contamination of the deoxyribonuclease I fraction by the serine-type proteases.
Yamaguchi
M
M
Department of Veterinary Anatomy and Cellular Biology, Ohio State University, Columbus 43210, USA.
Sanbuissho
A
A
Yamamoto
S
S
Tangkawattana
P
P
Sako
T
T
Motoyoshi
S
S
Oba
T
T
D000818:Animals; D016477:Artifacts; D002118:Calcium / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002645:Chickens; D003850:Deoxyribonuclease I / Q000302:isolation & purification / Q000494:pharmacology*; D004340:Drug Contamination; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008854:Microscopy, Electron; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000648:ultrastructure; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011817:Rabbits; D012697:Serine Endopeptidases / Q000494:pharmacology*; D015842:Serine Proteinase Inhibitors / Q000494:pharmacology
D015842:Serine Proteinase Inhibitors; D004533:Egtazic Acid; D010664:Phenylmethylsulfonyl Fluoride; D004492:Edetic Acid; D003850:Deoxyribonuclease I; D012697:Serine Endopeptidases; D008274:Magnesium; D005990:Glycerol; D002118:Calcium