title: Defects in the regulation of anti-DNA antibody production in aged lupus-prone (NZB x NZW)F1 mice: analysis of T-cell lymphokine synthesis.
abstract: (NZB x NZW)F1 (B/W) mice spontaneously develop a lupus-like syndrome characterized by an increased level of autoantibodies in old mice. We analysed the role of T cells in the regulation of anti-DNA antibody production by B cells in vitro as a function of age. In cultures of old mouse T and B cells, IgG and IgM anti-DNA antibodies were synthesized at high levels, in contrast to consistently lower amounts, particularly of IgG, measured in cultures of young mouse cells. Addition of young mouse T cells to old B cells inhibited IgG, but not IgM, anti-DNA production, whereas T cells from old mice stimulated IgG synthesis by young mouse B cells. Addition of supernatants harvested from concanavalin A (Con A)-stimulated T cells to B-cell cultures induced similar effects. Therefore, we evaluated possible modifications of lymphokine synthesis compared to that of the healthy NZW parent. T cells from old mice were able to secrete normal levels of interferon-gamma (IFN-gamma) and interleukin (IL)-10; however, secretion of IL-2 and IL-4 was dramatically decreased. Semi-quantitative polymerase chain reaction analysis of constitutive RNA messengers showed increased IFN-gamma levels in young and old B/W mice, and normal IL-10 mRNA levels in young and higher levels in old mice. Constitutive IL-2 and IL-4 mRNA were detected only after Con A stimulation and their levels decreased in old compared to young B/W mice; in particular IL-2 mRNA was considerably lower in old B/W than in control NZW mice. Taken together, these results suggest that, despite constitutive T-cell abnormalities, young B/W mice are able partially to control their lymphokine production, whereas aged mice exhibit a deficient synthesis, associated with an increased capacity to produce IFN-gamma.
authors.affiliation: Unité d'Immunocytochimie, CNRS URA 359, Institut Pasteur, Paris, France.
authors.forename: M N
authors.initials: MN
authors.lastname: Sato
authors.forename: P
authors.initials: P
authors.lastname: Minoprio
authors.forename: S
authors.initials: S
authors.lastname: Avrameas
authors.forename: T
authors.initials: T
authors.lastname: Ternynck
mesh_terms: D000375:Aging / Q000276:immunology; D000818:Animals; D000974:Antibodies, Antinuclear / Q000096:biosynthesis*; D001327:Autoimmune Diseases / Q000276:immunology*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000276:immunology; D017077:Culture Media, Conditioned; D005260:Female; D015870:Gene Expression; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008222:Lymphokines / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D051379:Mice; D008814:Mice, Inbred NZB; D012333:RNA, Messenger / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000974:Antibodies, Antinuclear; D017077:Culture Media, Conditioned; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008222:Lymphokines; D012333:RNA, Messenger; D003208:Concanavalin A