The Newcastle disease virus V protein binds zinc.
The V protein of Newcastle disease virus (NDV) is produced by the insertion of a single nontemplated G residue at a specific point during transcription of the phosphoprotein (P) gene, accessing a new reading frame upon translation. The V protein, in common with its counterpart in other paramyxoviruses contains a highly cysteine rich motif near the carboxyl terminus, suggestive of a zinc-binding domain. By constructing E. coli overexpression plasmids for the NDV P and V proteins, and monitoring the binding of 65ZnCl2 to proteins electroblotted onto nitrocellulose membranes, we have demonstrated that the V protein strongly binds zinc.
Steward
M
M
Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, U.K.
Samson
A C
AC
Errington
W
W
Emmerson
P T
PT
D000818:Animals; D001483:Base Sequence; D008969:Molecular Sequence Data; D009838:Oligodeoxyribonucleotides; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
C070065:36 kDa V protein, Newcastle disease virus; D009838:Oligodeoxyribonucleotides; C055691:P phosphoprotein, Newcastle disease virus; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D015032:Zinc