title: Excision of deoxyribose phosphate residues by DNA polymerase beta during DNA repair.
abstract: Eukaryotic DNA polymerase beta (pol beta) can catalyze DNA synthesis during base excision DNA repair. It is shown here that pol beta also catalyzes release of 5'-terminal deoxyribose phosphate (dRP) residues from incised apurinic-apyrimidinic sites, which are common intermediate products in base excision repair. The catalytic domain for this activity resides within an amino-terminal 8-kilodalton fragment of pol beta, which comprises a distinct structural domain of the enzyme. Magnesium is required for the release of dRP from double-stranded DNA but not from a single-stranded oligonucleotide. Analysis of the released products indicates that the excision reaction occurs by beta-elimination rather than hydrolysis.
authors.affiliation: Department of Radiation Oncology, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: K
authors.initials: K
authors.lastname: Kim
mesh_terms: D000818:Animals; D001080:Apurinic Acid; D001483:Base Sequence; D001665:Binding Sites; D004247:DNA / Q000378:metabolism*; D011088:DNA Ligases / Q000378:metabolism; D004256:DNA Polymerase I / Q000378:metabolism*; D004260:DNA Repair; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004492:Edetic Acid / Q000494:pharmacology; D006868:Hydrolysis; D008190:Lyases / Q000378:metabolism; D008969:Molecular Sequence Data; D011119:Polynucleotides; D017434:Protein Structure, Tertiary; D051381:Rats; D012267:Ribosemonophosphates / Q000378:metabolism*; D014982:Xenopus laevis
chemical_list: D011119:Polynucleotides; D012267:Ribosemonophosphates; C001790:apyrimidinic acid; D001080:Apurinic Acid; D004247:DNA; D004492:Edetic Acid; D004256:DNA Polymerase I; D043223:Deoxyribonuclease IV (Phage T4-Induced); D008190:Lyases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D011088:DNA Ligases