[Chromosome microdissection: applications and prospects].
A new microdissection and microcloning procedure of human banded chromosomes has been described. These micro-techniques allow the construction of band-specific DNA libraries, providing a large number of DNA probes for the identification of gene sequences. These technologies associated with fluorescence in situ hybridization studies are particularly efficient to analyse chromosomal regions involved in human genetic diseases including cancer.
Jonveaux
P
P
Laboratoire Central d'Hématologie, Hôpital Saint-Louis, Paris, France.
D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping / Q000379:methods* / Q000639:trends; D003001:Cloning, Molecular / Q000379:methods*; D004210:Dissection; D005544:Forecasting; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction
La microdissection chromosomique: applications et perspectives.