title: The influence of gamma inulin and Algammulin on the immune response in sheep to a recombinant antigen of Taenia ovis.
abstract: Gamma inulin and Algammulin, two new adjuvants, were examined and compared with alum and Freund's Complete/Incomplete Adjuvant (FCA/FIA), for potentiation of cell-mediated immunity (CMI) and humoral immunity in sheep to a recombinant Taenia ovis antigen. The ability to protect sheep when challenged with live T. ovis eggs was also assessed. The results showed that gamma inulin and Algammulin induced a CMI response which was comparable to the FCA/FIA and alum groups and significantly higher than the control saline group. While gamma inulin, Algammulin and alum performed similarly and induced a significantly higher humoral immune response than the saline group. FCA/FIA elicited a much higher humoral immune response. Algammulin did not show the synergistic effect seen in mice and performed similarly to gamma inulin and alum alone. All the adjuvant groups induced significantly higher IgG1 and IgG2 levels than the saline group and they all favoured IgG1 production. When the sheep were challenged with live T. ovis eggs, at 25 weeks after primary immunization, the only group to show significant protection was the one which received FCA/FIA.
authors.affiliation: Biotechnology Programme, School of Biological and Environmental Sciences, Murdoch University, Western Australia.
authors.forename: H S
authors.initials: HS
authors.lastname: Deol
authors.forename: D G
authors.initials: DG
authors.lastname: Palmer
authors.forename: T
authors.initials: T
authors.lastname: Dunsmore
authors.forename: P R
authors.initials: PR
authors.lastname: Carnegie
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000534:Alum Compounds / Q000494:pharmacology; D000704:Analysis of Variance; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000947:Antigens, Helminth / Q000276:immunology* / Q000494:pharmacology*; D002455:Cell Division; D004338:Drug Combinations; D004797:Enzyme-Linked Immunosorbent Assay; D005620:Freund's Adjuvant / Q000494:pharmacology; D007111:Immunity, Cellular / Q000187:drug effects; D007444:Inulin / Q000494:pharmacology*; D008297:Male; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology; D012756:Sheep; D013621:Taenia / Q000276:immunology*; D013622:Taeniasis / Q000276:immunology* / Q000517:prevention & control*
chemical_list: D000276:Adjuvants, Immunologic; C070342:Algammulin; D000534:Alum Compounds; D000947:Antigens, Helminth; D004338:Drug Combinations; D011994:Recombinant Proteins; C041524:aluminum sulfate; D007444:Inulin; D005620:Freund's Adjuvant