title: Endodontic cements induce alterations in the cell cycle of in vitro cultured osteoblasts.
abstract: The effects of endodontic cements on the cell cycle of MG63 osteoblasts cultured in vitro have been examined. Three groups of compounds were tested. Group I encompassed zinc oxide- and eugenol-based cements (Tubliseal, Argoseal, N2), group II consisted of cements with a phenol group other than eugenol (AH26, Forfenan, Methode R/R), and group III included CaOH-based cements (Biocalex, Endocalex). The cell cycle of MG63 cells was analyzed by flow cytometry; the DNA content was evaluated by means of the propidium iodide uptake method, whereas the proportion of cells in the S phase was defined by the incorporation of bromodeoxyuridine later revealed by a specific antibody. The results showed that some root canal sealers could hamper the periapex healing processes by inhibiting the cell proliferation through a selective action on different phases of the cell cycle.
authors.affiliation: Laboratory for Biocompatibility Research on Implant Materials, Istituti Ortopedici Rizzoli, Bologna, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Granchi
authors.forename: S
authors.initials: S
authors.lastname: Stea
authors.forename: G
authors.initials: G
authors.lastname: Ciapetti
authors.forename: D
authors.initials: D
authors.lastname: Cavedagna
authors.forename: S
authors.initials: S
authors.lastname: Stea
authors.forename: A
authors.initials: A
authors.lastname: Pizzoferrato
mesh_terms: D000704:Analysis of Variance; D002126:Calcium Hydroxide / Q000633:toxicity; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis; D003738:Dental Cements / Q000633:toxicity*; D005434:Flow Cytometry; D006801:Humans; D010006:Osteoblasts / Q000187:drug effects*; D010484:Periapical Granuloma / Q000209:etiology; D012387:Root Canal Filling Materials / Q000633:toxicity*; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
chemical_list: D003738:Dental Cements; D012387:Root Canal Filling Materials; D015035:Zinc Oxide-Eugenol Cement; D004247:DNA; D002126:Calcium Hydroxide