[In vitro analysis for cellular toxicity of polychlorinated biphenyls(PCBs) and 2, 3, 4, 7, 8-pentachlorodibenzofuran (PCDF) on HeLa cell proliferation(III)--the effect of cytoactivator and antilipemic agents on cell toxicity].
We investigated the cell toxicity of polychlorinated biphenyls (PCBs) and 2, 3, 4, 7, 8-pentachlorodibenzofuran (PCDF) as indicators of the optical density (280nm) which is total protein in HeLa cells. Furthermore, the reductive action of cytoactivator and antilipemic agents on the PCBs and PCDF toxicity were evaluated. The quantity of total cellular protein increased to 20% with the addition of sodium dextran sulfate (2.5%) at the presence of PCBs, and 25% in the case of PCDF. However, the slope of the curve of cell proliferation of HeLa cells at the presence of PCBs or PCDF became to overlap with a control group at the presence of any other drugs except for sodium dextran sulfate. These results mean that PCBs and PCDF cell toxicity were suppressed a little by sodium dextran sulfate, but the case of other cytoactivator and antilipemic agents did not.
Matsuoka
K
K
Division of Pharmacology, National Kikuchi Hospital for Mental and Nervous Diseases, Kumamoto.
Tsukazaki
N
N
Yoshida
H
H
D001572:Benzofurans / Q000037:antagonists & inhibitors / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016264:Dextran Sulfate / Q000494:pharmacology*; D006367:HeLa Cells / Q000166:cytology; D006801:Humans; D000960:Hypolipidemic Agents / Q000494:pharmacology*; D011078:Polychlorinated Biphenyls / Q000037:antagonists & inhibitors / Q000633:toxicity*
D001572:Benzofurans; D000960:Hypolipidemic Agents; D016264:Dextran Sulfate; D011078:Polychlorinated Biphenyls; C038890:2,3,4,7,8-pentachlorodibenzofuran