Nuclear receptors for 1,25-dihydroxy-22-oxavitamin D3 (OCT) and 1,25-dihydroxyvitamin D3 in gastric gland neck mucous cells and gastrin enteroendocrine cells.
22-Oxacalcitriol the analog with low calcemic effect and the original hormone 1,25(OH)2 vitamin D3 were localized by autoradiography in mouse stomach at different time intervals after intravenous injection. Both compounds showed a distinct nuclear concentration and retention in neck mucous cells of gastric and pyloric glands, and in dispersed endocrine cells in the antrum region. When the nuclear binding of radioactively labelled compound was compared between gastric neck cells and duodenal absorptive cells, binding was low but sustained in neck cells. Peak uptake after the injection was between 8 and 12 h in neck cells, but between 15 min and 30 min in duodenal villous epithelium. In the duodenum, weak nuclear labelling appeared at 8 h and was undetectable at 12 h under the conditions of the experiment. Nuclear labelling of neck cells remained detectable at 12 h and even after 24 h, similarly for both OCT and 1,25(OH)2 vitamin D3. These results suggest that the stomach is an important target tissue for vitamin D and its analog OCT. Regulation of neck cell functions is suggested, such as proliferation and differentiation of surface epithelium and gastric gland epithelium, and neck cell secretion of acidic mucus. Regulation is also indicated of G-cell gastrin secretion associated with gastrin paracrine effects on parietal cell HCl and intrinsic factor secretion, chief cell pepsinogen secretion, neck cell proliferation, as well as endocrine effects on systemic calcium homeostasis.
Stumpf
W E
WE
Research Laboratories, Chugai Pharmaceutical Company, Tokyo, Japan.
Hayakawa
N
N
Koike
N
N
Hirate
J
J
Okazaki
A
A
D000818:Animals; D001345:Autoradiography; D002117:Calcitriol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D008297:Male; D051379:Mice; D018167:Receptors, Calcitriol / Q000032:analysis*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism
D018167:Receptors, Calcitriol; D018160:Receptors, Cytoplasmic and Nuclear; D002117:Calcitriol; C051883:maxacalcitol