Complete amino acid sequence of a lectin-related 16.5-kDa protein isolated from fruit bodies of a lectin-deficient strain of Pleurotus cornucopiae.
The complete amino acid sequence of a lectin-related 16.5-kDa protein (PCL-RP) isolated from fruit bodies of a lectin-deficient strain of P. cornucopiae is presented. The sequences of six out of the seven peptides generated by digestion with lysylendopeptidase and four of the five peptides generated by cyanogen bromide cleavage were completely analyzed. Overlapping peptides were obtained by arginylendopeptidase digestion. PCL-RP was a single-chain protein consisting of 144 amino acid residues and its N-terminal serine was blocked with acetate. A proline-rich sequence was found in the carboxyl terminal portion. The N-terminal sequence of PCL-RP showed some homology with those of two known Basidiomycete lectins.
Oguri
S
S
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University, Matsudo, Japan.
Nagata
Y
Y
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D005656:Fungal Proteins / Q000032:analysis* / Q000302:isolation & purification; D006868:Hydrolysis; D037102:Lectins / Q000032:analysis* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010455:Peptides / Q000302:isolation & purification; D011124:Polyporaceae / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid
D000596:Amino Acids; D005656:Fungal Proteins; D037102:Lectins; D010455:Peptides