Enzyme- and mineralocorticoid receptor-controlled electrogenic Na+ absorption in human rectum in vitro.
In vivo electrogenic Na+ absorption (JeNa) in the human rectum is controlled by acute variation of aldosterone in nanomolar concentration range. In this study we report both the induction of JeNa in human rectum epithelium by nanomolar aldosterone added in vitro and the enzymatic control of glucocorticoid action on JeNa. JeNa was measured as amiloride-sensitive short-circuit current 8 h after addition of the respective steroid. Aldosterone (10 nM) caused JeNa of 5.7 +/- 1.4 mumol.h-1.cm-2. Cortisol in the same concentration did not induce significant JeNa. Because cortisol is readily inactivated by 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), the true mineralocorticoid activity of cortisol was evaluated after inhibition of 11 beta-HSD by carbenoxolone. Carbenoxolone alone did not exhibit mineralocorticoid activity. If cortisol (10 nM) was given together with carbenoxolone (1 microM), the resulting JeNa (4.5 +/- 0.4 mumol.h-1.cm-2) was not significantly different from that after 10 nM aldosterone, indicating equal intrinsic mineralocorticoid activity of cortisol and aldosterone. The same mechanisms were found in rat late distal colon. Kinetic data of carbenoxolone at 10 nM cortisol resulted in a Michaelis constant of 0.3 microMs, maximal absorption of 8.4 mumol.h-1.cm-2, and a Hill coefficient of 1.8. The effects of carbenoxolone and glycyrrhetinic acid did not differ. We conclude that JeNa is under complete control of mineralocorticoid action. "Spontaneous" JeNa in the beginning of the in vitro period can be explained by elevated steroid levels before tissue removal.(ABSTRACT TRUNCATED AT 250 WORDS)
Epple
H J
HJ
Institut für Klinische Physiologie, Universitätsklinikum Benjamin Franklin, Freie Universität Berlin, Germany.
Schulzke
J D
JD
Schmitz
H
H
Fromm
M
M
D042842:11-beta-Hydroxysteroid Dehydrogenases; D000042:Absorption; D000368:Aged; D000450:Aldosterone / Q000494:pharmacology; D002229:Carbenoxolone / Q000494:pharmacology; D003106:Colon / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004594:Electrophysiology; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D006913:Hydroxysteroid Dehydrogenases / Q000037:antagonists & inhibitors / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D018161:Receptors, Mineralocorticoid / Q000502:physiology*; D012007:Rectum / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
D018161:Receptors, Mineralocorticoid; D000450:Aldosterone; D012964:Sodium; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases; D002229:Carbenoxolone; D006854:Hydrocortisone