Metastasis of C1300 and TBJ murine neuroblastomas correlates with expression of matrix metalloproteinases.
Because metalloproteinases, specifically type IV collagenases, may mediate metastasis, 72- and 92-kDa collagenase activities were evaluated in two murine neuroblastoma cell lines: non-metastatic C1300 and metastatic TBJ. Zymogram analysis demonstrated that 72- and 92-kDa collagenases were associated only with metastatic TBJ. Three human neuroblastoma cell lines were then evaluated by zymogram and Western blot analyses: 72-kDa collagenase was found in metastatic SK-N-SH and IMR-32, but not in SK-N-MC, which may be a peripheral neuroectodermal tumor and not a neuroblastoma. Therefore, 72- and 92-kDa collagenases may be markers of metastasis in neuroblastoma and may aid in differentiation from other small blue cell tumors.
Fowler
C
C
Department of Surgery, University of Kentucky Medical Center, Lexington 40536, USA.
Zimmer
C
C
Boghaert
E
E
Thomas
M
M
Zimmer
S
S
D000818:Animals; D017364:Collagenases / Q000378:metabolism*; D005260:Female; D006801:Humans; D051379:Mice; D008805:Mice, Inbred A; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009447:Neuroblastoma / Q000201:enzymology* / Q000401:mortality / Q000556:secondary*; D013045:Species Specificity; D014407:Tumor Cells, Cultured
D017364:Collagenases