title: Occurrence of cytochrome P450c17 mRNA and dehydroepiandrosterone biosynthesis in the rat gastrointestinal tract.
abstract: We have investigated 17 alpha-hydroxylase and C17,20-lyase activities and the presence of cytochrome P450c17 mRNA in the esophagus, stomach, duodenum, and colon of adult rats of both sexes. All tissues converted [4-14C]pregnenolone mainly to dehydroepiandrosterone (DHEA) through the 5-ene-3 beta-hydroxysteroid route as opposed to the 4-ene-3-ketosteroid pathway in a control testicular incubate. Synthesis of dehydroepiandrosterone was particularly high in the duodenum and was found to be lower in the stomach, colon and esophagus, in decreasing order. 20 alpha-Hydroxypregnenolone and progesterone were also formed primarily by the esophagus and colon, respectively. P450c17 mRNA was demonstrated by ribonuclease protection assay in the stomach and duodenum, but not in esophagus and colon. However, a 335 bp-long cDNA fragment, whose sequence corresponded to that of rat P450c17 cDNA, was amplified by reverse transcription (RT) and polymerase chain reaction (PCR) from the poly(A)+ RNAs of all four tissues. This result was further confirmed by Southern blotting using a 794-bp testicular probe. The complete sequence of P450c17 cDNA in the stomach and duodenum was identical to that reported for rat testis P450c17 cDNA. No amplification and no positive signal in Southern blotting were observed with the total RNAs from adult male adrenal and spleen, which were taken as negative controls since they had been previously found unable to form androgens from pregnenolone. Although the levels of transcription in gonads, duodenum and stomach were found to be equivalent, as indicated by the RNase protection assay and semiquantitative RT-PCR assay, P450c17 enzyme activity was much higher in the testis, pointing at a possible dissimilarity in the respective rates of mRNA translation. Thus, P450c17 is differentially expressed in the rat gastrointestinal tract, where it leads to the synthesis of the sex steroid precursor DHEA, especially in the duodenum and stomach.
authors.affiliation: Department of Biology, University of Padova, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Dalla Valle
authors.forename: J
authors.initials: J
authors.lastname: Couët
authors.forename: Y
authors.initials: Y
authors.lastname: Labrie
authors.forename: J
authors.initials: J
authors.lastname: Simard
authors.forename: P
authors.initials: P
authors.lastname: Belvedere
authors.forename: C
authors.initials: C
authors.lastname: Simontacchi
authors.forename: F
authors.initials: F
authors.lastname: Labrie
authors.forename: L
authors.initials: L
authors.lastname: Colombo
mesh_terms: D000446:Aldehyde-Lyases / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D003106:Colon / Q000201:enzymology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry; D003687:Dehydroepiandrosterone / Q000096:biosynthesis*; D004064:Digestive System / Q000201:enzymology*; D004386:Duodenum / Q000201:enzymology; D004947:Esophagus / Q000201:enzymology; D005260:Female; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011284:Pregnenolone / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013254:Steroid 17-alpha-Hydroxylase / Q000235:genetics* / Q000378:metabolism*; D013270:Stomach / Q000201:enzymology
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D003687:Dehydroepiandrosterone; D011284:Pregnenolone; D003577:Cytochrome P-450 Enzyme System; D013254:Steroid 17-alpha-Hydroxylase; D000446:Aldehyde-Lyases