Catalase inactivation following photosensitization with tetrasulfonated metallophthalocyanines.
Catalase (CAT) in solution or incorporated in erythrocytes and K562 leukemic cells is inactivated during photosensitization with tetrasulfonated metallophthalocyanines (MePcS4). The effect of added scavengers and D2O showed that both singlet oxygen and free radical species are involved in this process. Evidence was found that direct interactions of ground or excited-stated photosensitizer with CAT are not responsible for CAT inactivation. Specific techniques to probe early damage to the CAT structure involved optical and EPR spectroscopy, HPLC and polyacrylamide gel electrophoresis analyses. Different primary events of photosensitized protein damage included oxidation of cysteine residues as well as other amino acids, as demonstrated by the formation of carbon-centered free radicals and the loss of absorbance at lambda = 275 nm. In parallel, we detected degradation of the CAT heme groups, accompanied by release of Fe(II) ions in solution. These combined phenomena initiate cross-linkages between CAT subunits and subsequent degradation of the protein with formation of irreversible aggregates in solution. Phthalocyanine-mediated photoinactivation of cell-bound CAT results in loss of protection against accumulating H2O2, providing an additional pathway of phototoxicity.
Gantchev
T G
TG
Department of Nuclear Medicine and Radiobiology, Faculty of Medicine, University of Sherbrooke, Quebec, Canada.
van Lier
J E
JE
D000818:Animals; D002374:Catalase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D002417:Cattle; D003545:Cysteine / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000201:enzymology; D005609:Free Radicals; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000494:pharmacology*; D054833:Isoindoles; D008099:Liver / Q000201:enzymology; D008670:Metals / Q000737:chemistry / Q000494:pharmacology*; D010084:Oxidation-Reduction; D017319:Photosensitizing Agents / Q000494:pharmacology*; D051381:Rats; D012996:Solutions; D013451:Sulfonic Acids / Q000737:chemistry; D014407:Tumor Cells, Cultured
D005609:Free Radicals; D007211:Indoles; D054833:Isoindoles; D008670:Metals; D017319:Photosensitizing Agents; D012996:Solutions; D013451:Sulfonic Acids; D002374:Catalase; D003545:Cysteine; C013647:phthalocyanine