Advantage of a rapid extraction method of HIV1 DNA suitable for polymerase chain reaction.
We describe a new protocol for extraction of DNA suitable for HIV1 gene amplification from clinical samples using "Chelex-100" chelating resin. Comparison was made with the classic proteinase K extraction method; 154 specimens were extracted with both methods and subjected to PCR (polymerase chain reaction). The Chelex-100 procedure optimized the yield of DNA recovery and minimized contamination due to sample manipulation. It decreased false negative results due to PCR inhibitors. A DNA sample suitable for use in PCR was obtained in 30 minutes. Chelex-100 treatment is a simple, rapid and low-cost method for DNA extraction in clinical laboratories.
Vignoli
C
C
Laboratoire de Virologie, Hôpital de la Timone, Marseille, France.
de Lamballerie
X
X
Zandotti
C
C
Tamalet
C
C
de Micco
P
P
D002478:Cells, Cultured; D002614:Chelating Agents; D004279:DNA, Viral / Q000302:isolation & purification*; D019286:Endopeptidase K; D015658:HIV Infections / Q000097:blood / Q000134:cerebrospinal fluid / Q000821:virology*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012117:Resins, Synthetic; D012697:Serine Endopeptidases
D002614:Chelating Agents; D004279:DNA, Viral; D012117:Resins, Synthetic; C024997:Chelex 100; D012697:Serine Endopeptidases; D019286:Endopeptidase K