The linoleic acid metabolite 13-HODE modulates degranulation of human polymorphonuclear leukocytes.
The effect of the linoleic acid metabolite 13-hydroxyoctadecadienoic acid human polymorphonuclear leukocytes (PMNs) was investigated by measuring the expression of CD11b and CD67 on the plasma membrane. 13-HODE (5 microM) by itself induced degranulation of PMNs, but to a lesser extent as compared to PAF and fMLP. In addition, 13-HODE was found to inhibit the PAF-induced degranulation whereas an additive effect on the fMLP-induced PMNs degranulation was observed. These results indicate that 13-HODE can play a modulatory role in degranulation of PMNs.
van de Velde
M J
MJ
Department of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands.
Engels
F
F
Henricks
P A
PA
Bloemen
P G
PG
Nijkamp
F P
FP
D015703:Antigens, CD / Q000096:biosynthesis; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D015550:Cell Degranulation / Q000187:drug effects*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D008041:Linoleic Acids / Q000494:pharmacology*; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000502:physiology*; D010972:Platelet Activating Factor / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D015815:Cell Adhesion Molecules; D008041:Linoleic Acids; D016177:Macrophage-1 Antigen; D008562:Membrane Glycoproteins; D010972:Platelet Activating Factor; C024348:13-hydroxy-9,11-octadecadienoic acid; D009240:N-Formylmethionine Leucyl-Phenylalanine