A role for active oxygen species as second messengers in the induction of alternative oxidase gene expression in Petunia hybrida cells.
Incubation of Petunia hybrida cells with H2O2 leads to an increase in alternative oxidase activity measured after 24 h. This increased activity is accompanied by an increase in alternative oxidase protein. A model is presented for the regulation of alternative oxidase protein synthesis in which active oxygen species and especially H2O2 play a crucial role as second messengers in the signal transducing pathway from the mitochondria to the nucleus. It is proposed that also the induction of the alternative oxidase by salicylic acid is mediated via H2O2.
Wagner
A M
AM
Department of Physiology and Biochemistry of Plants, Vrije Universiteit, Amsterdam, The Netherlands.
D002478:Cells, Cultured; D004790:Enzyme Induction / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism / Q000502:physiology; D024101:Mitochondrial Proteins; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D018521:Plant Physiological Phenomena; D010940:Plant Proteins / Q000096:biosynthesis* / Q000378:metabolism; D010944:Plants / Q000201:enzymology*; D017382:Reactive Oxygen Species; D012459:Salicylates / Q000494:pharmacology; D020156:Salicylic Acid; D015290:Second Messenger Systems / Q000502:physiology*; D000099188:Alternative Oxidase
D006861:Hydrogen Peroxide; D024101:Mitochondrial Proteins; D010088:Oxidoreductases; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D012459:Salicylates; D020156:Salicylic Acid; D000099188:Alternative Oxidase