Differential expression of the chicken Pax-1 and Pax-9 gene: in situ hybridization and immunohistochemical analysis.
We report the cloning, partial sequence analysis, and spatiotemporal expression of the chicken Pax-1 (chPax-1) and Pax-9 (chPax-9) gene, two closely related members of the paired box-containing (PAX) gene family. The chPax-1 gene encodes RNAs of 2.0 and 4.3 kb and a 42 kD protein while the gene products of chPax-9 are represented by 1.9 and 3.1 kb transcripts and a 39 kD protein. In situ hybridization and immunohistochemical analyses reveal chPax-1 expression in the developing pectoral girdle, in cells of the ventral part of sclerotomes, in sclerotome cells of the perichordal tube, and, later in development, in sclerotome-derived cells of the intervertebral disks. Other chPax-1 expression domains detected in the mesenchyme surrounding the atlas and axis and in chondrocytes of immature vertebral bodies, so far unreported for mouse Pax-1, correlate with as yet unexplained malformations in the mouse Pax-1 mutant undulated and Undulated-short tail. Overlapping expression of chPax-1 and chPax-9 is detected in epithelial cells of the embryonic and adult thymus and in cells of the developing intervertebral disks. Unlike chPax-1, however, chPax-9 is not expressed in those perichordal sclerotome cells which are thought to give rise to vertebral bodies. Furthermore, chPax-9 gene products are detected in circumscribed areas of mesenchyme in the metatarsus and in entodermal derivatives, i.e., in the lining epithelium of the developing pharynx and of the embryonic and adult esophagus.
Peters
H
H
Institut für Molekularbiologie und Tumorforschung, Philipps-Universität, Marburg, Federal Republic of Germany.
Doll
U
U
Niessing
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002642:Chick Embryo; D002645:Chickens; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004947:Esophagus / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D051764:PAX9 Transcription Factor; D051761:Paired Box Transcription Factors; D010614:Pharynx / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013131:Spine / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D013950:Thymus Gland / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*
D017931:DNA Primers; D004268:DNA-Binding Proteins; D051764:PAX9 Transcription Factor; C497323:PAX9 protein, human; D051761:Paired Box Transcription Factors; D014157:Transcription Factors; C070835:PAX1 transcription factor