Properties of a transplasma membrane redox system of Phanerochaete chrysosporium.
A transplasma membrane redox system of Phanerochaete chrysosporium was studied using ferricyanide, a membrane-impermeable electron acceptor. Rates of reduction were dependent upon initial ferricyanide concentration and mycelial mass. Specific activities of 12 +/- 2 nmol/min/mg mycelia (dry wt) were consistently obtained using nutrient-sufficient mycelia at pH 8.0 and 10 mM ferricyanide. Upon nutrient limitation (either carbon or nitrogen), activity decreased. Reduction was inhibited by carbonyl cyanide m-chloromethoxyphenyl hydrazone, 2,4-dinitrophenol, and sodium azide but not by potassium cyanide at 100 nmol/mg mycelia. Ferricyanide reduction and proton export rates increased with pH above the physiological pH for the fungus. The stimulation in proton exported by the addition of ferricyanide was equal to the rate of ferricyanide reduced at pH 8.0 when Hepes buffer was used. The relevance of these findings with regard to the physiological pH optimum of the fungus and the metabolism of pollutants by this fungus is discussed.
Stahl
J D
JD
Biotechnology Center, Utah State University, Logan 84322-4705, USA.
Aust
S D
SD
D002462:Cell Membrane / Q000378:metabolism*; D005292:Ferricyanides / Q000378:metabolism*; D005658:Fungi / Q000378:metabolism*; D010084:Oxidation-Reduction
D005292:Ferricyanides; C007931:hexacyanoferrate III