title: Physical mapping and characterization of a catabolic plasmid from the deep-subsurface bacterium Sphingomonas sp. strain F199.
abstract: A supercoiled 180-kb plasmid, pNL1, has been isolated from the deep-subsurface, chemoheterotrophic Sphingomonas sp. strain F199, and a physical map was generated. Analysis of a pNL1-derived cosmid library indicated that catechol 2,3-dioxygenase activity was linked to two distinct regions of the plasmid. Thus, the genes for aromatic catabolism in this Sphingomonas strain are, at least in part, plasmid encoded.
authors.affiliation: Pacific Northwest Laboratory, Richland, Washington 99352, USA.
authors.forename: L C
authors.initials: LC
authors.lastname: Stillwell
authors.forename: S J
authors.initials: SJ
authors.lastname: Thurston
authors.forename: R P
authors.initials: RP
authors.lastname: Schneider
authors.forename: M F
authors.initials: MF
authors.lastname: Romine
authors.forename: J K
authors.initials: JK
authors.lastname: Fredrickson
authors.forename: J D
authors.initials: JD
authors.lastname: Saffer
mesh_terms: D050557:Catechol 2,3-Dioxygenase; D049308:Dioxygenases; D015964:Gene Expression Regulation, Bacterial; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology / Q000235:genetics*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping
chemical_list: D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase