In vitro viability of vitrified mouse morulae thawed at different temperatures.
The effect of thawing temperature on in vitro development of vitrified mouse morulae was investigated. The embryos were vitrified in a solution based on ethylene glycol as cryoprotectant, and Ficoll as macromolecule to assist vitrification. They were then thawed at 20 degrees, 37 degrees and 48 degrees C for 6 sec and at 48 degrees C for 2 sec. Among groups, there was no significant difference on the development at 72 h of culture when embryos were thawed at 20 degrees, 37 degrees C for 6 sec or 48 degrees C for 2 sec. At 48 h of culture the embryos thawed at lower temperature had a reduced resumption (69.5%) while the embryos thawed at 37 degrees and 48 degrees C for 2 sec had a higher resumption rate (80.0% and 82.5%). It was concluded that a high development in vitro of vitrified mouse morulae can be obtained at three different temperatures of thawing, although at higher temperatures there seems to be a tendency of an earlier resumption development.
Dattena
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M
Istituto Zootecnico e Caseario per la Sardegna, Olmedo (SS).
Sanna
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V
Cappai
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P
D000818:Animals; D001755:Blastocyst; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000633:toxicity; D019855:Ethylene Glycol; D005026:Ethylene Glycols / Q000633:toxicity; D005260:Female; D005362:Ficoll / Q000633:toxicity; D051379:Mice; D009028:Morula / Q000187:drug effects / Q000502:physiology*; D012996:Solutions / Q000633:toxicity; D013395:Sucrose / Q000633:toxicity; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods*
D003451:Cryoprotective Agents; D005026:Ethylene Glycols; D012996:Solutions; D005362:Ficoll; D013395:Sucrose; D019855:Ethylene Glycol