Amino acid transport after transient global ischemia in rats: quantitative autoradiographic study using 3-[125I]iodo-alpha-methyl-L-tyrosine.
We studied the influence of reperfusion on amino acid transport of the brain after transient global ischemia in rats. The animals were subjected to 30-min four-vessel occlusion according to the procedures developed by Pulsinelli prior to recirculation for 3, 6, 24, 48 and 72 h. We used 3-[125I]iodo-alpha-methyl-L-tyrosine as an autoradiographic tracer for selective cerebral amino acid transport maker. Following 30-min global ischemia, uptakes of 3-[125I]iodo-alpha-methyl-L-tyrosine were significantly (P < 0.05) lower in substantia nigra, striatum and ventral tegmental area (6, 24, 48 and 72 h post-reperfusion), but significantly (P < 0.05) higher in cortex and thalamus (3 and 6 h post-reperfusion). The influence of transient global ischemia on cerebral amino acid transport manifested region-specific three different patterns; namely, suppression, acceleration and no change in amino acid transport. The influence of transient ischemia on catecholamine-synthesizing brain sites is most remarkable.
Yoshizumi
H
H
Department of Neurosurgery, Faculty of Medicine, Kyoto University, Japan.
Fujibayashi
Y
Y
Kikuchi
H
H
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D001692:Biological Transport; D001921:Brain / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000000981:diagnostic imaging / Q000378:metabolism*; D008297:Male; D008781:Methyltyrosines / Q000493:pharmacokinetics*; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion
D000596:Amino Acids; D008781:Methyltyrosines; C030855:3-iodo-alpha-methyltyrosine