Aspirin by virtue of its acidic property may act as teratogen in early chick embryo.
Aspirin (acetylsalicyclic acid) was dissolved either in normal saline or in phosphate buffer and was used in two doses to find out whether teratogenic potential of aspirin in chick blastoderm model is due to its acidic property or due to drug action. Drug was injected sub-blastodermally by window technique in fresh embryonated eggs after 17 hours of incubation at 39 degrees C. Eggs were re-incubated and harvested at 40 hours. Normal development of embryos was seen with normal saline and percentage of normal embryos with 30 micrograms (pH-3.19) and 120 micrograms (pH-2.64) aspirin was 31.7 and 4.9 respectively. Buffer produced 80.8% normal embryos and buffered 30 micrograms (pH-6.87) and 120 micrograms (pH-6.69) aspirin produced 67.7% and 30.8% normal embryos respectively. Changing the pH of aspirin to near neutral decreased the defect induced by aspirin but a significant effect of aspirin was observed at higher dose which could be independent of pH action.
Kotwani
A
A
Department of Pharmacology, Maulana Azad Medical College, New Delhi.
Mehta
V L
VL
Iyengar
B
B
D000014:Abnormalities, Drug-Induced / Q000473:pathology*; D000818:Animals; D001241:Aspirin / Q000633:toxicity*; D001756:Blastoderm / Q000187:drug effects*; D002021:Buffers; D002642:Chick Embryo / Q000187:drug effects* / Q000254:growth & development; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D009436:Neural Tube Defects / Q000139:chemically induced; D010710:Phosphates / Q000737:chemistry; D012462:Saline Solution, Hypertonic / Q000737:chemistry; D016707:Tissue Fixation
D002021:Buffers; D010710:Phosphates; D012462:Saline Solution, Hypertonic; D001241:Aspirin