Inhibition of macrophage procoagulant activity with lipids reduces intra-abdominal abscess formation in mice.
Macrophage procoagulant activity (PCA) has been proposed as a critical element in the formation of intra-abdominal abscesses. We tested the ability of local and systemic lipids (a known PCA inhibitor in vitro) to alter both peritoneal macrophage PCA and mixed Escherichia coli/Bacteroides fragilis intra-abdominal abscess formation in vivo. Lipids given intraperitoneally inhibited measured inducible peritoneal macrophage PCA for 8 h, and, in other animals, significantly decreased the number of abscesses formed and prevented abscesses in 29% of animals. Parallel experiments using subcutaneous intralipids did not show these effects. These results support the hypothesis that macrophage PCA produced locally is critical for intraperitoneal abscess formation.
Sawyer
R G
RG
Department of Surgery, University of Michigan, Ann Arbor, USA.
Pruett
T L
TL
D000005:Abdomen; D000038:Abscess / Q000517:prevention & control*; D000818:Animals; D001779:Blood Coagulation Factors / Q000037:antagonists & inhibitors*; D007263:Infusions, Parenteral; D007279:Injections, Subcutaneous; D008055:Lipids / Q000008:administration & dosage / Q000494:pharmacology*; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
D001779:Blood Coagulation Factors; D008055:Lipids; C045072:macrophage procoagulant activity