The two-color analysis of the T-lymphocyte subsets in experimental allergic neuritis in rats.
We examined the T-cell subsets in the peripheral blood, spleen and lymph nodes during the course of experimental allergic neuritis (EAN) by using two-color analysis. In the acute phase, the percentages of CD4+ major histocompatibility complex (MHC)-II+ cells and CD8+ MHC-II+ cells in the lymph nodes of EAN rats were significantly higher than in the control rats. In the recovery phase, the percentage of CD4+ CD45RC+ cells and CD8+ CD45RC+ cells in the lymph nodes in EAN rats were significantly higher than in the control rats. However, significant changes of the T-cell subsets were not detected in either the spleen or the peripheral blood during the course of EAN. These results suggest that CD4+ MHC-II+ cells, CD8+ MHC-II+ cells, CD4+ CD45RC+ cells and CD8+ CD45RC+ cells may play a role in the course of EAN. The relationship between these double staining cells and EAN is discussed.
Takai
H
H
Division of Neurology, Faculty of Medicine, Tottori University, Yonago, Japan.
Araga
S
S
Funamoto
K
K
Nakayasu
H
H
Takahashi
K
K
D000704:Analysis of Variance; D000818:Animals; D000911:Antibodies, Monoclonal; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D008198:Lymph Nodes / Q000276:immunology; D018655:Lymphocyte Count; D008285:Major Histocompatibility Complex / Q000276:immunology; D009444:Neuritis, Autoimmune, Experimental / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*
D000911:Antibodies, Monoclonal