Identification of salivary proteins inhibiting herpes simplex virus 1 replication.
Salivary proteins play an important role in the maintenance of the oral ecology. Previous studies have indicated that human submandibular-sublingual and parotid salivas can selectively suppress the in vitro infectivity of herpes simplex virus 1. The purpose of this study was to identify the salivary components in human submandibular-sublingual saliva that modulate in vitro infectivity. Assessment of the interaction of viral particles with salivary components was accomplished using an in vitro solid-phase assay. These experiments revealed that herpes simplex virus particles selectively interact with the members of the salivary proline-rich protein and cystatin families. Subsequent yield reduction assays demonstrated the ability of proline-rich proteins and salivary cystatins to inhibit the viral replication, with basic proline-rich peptides being more effective. Subsequent assays suggest that basic proline-rich peptides reduced the virus titer by interfering with penetration and/or cellular processing of virus within the target cell. Collectively, these results further suggest that salivary proteins have an important role in the host defense mechanism against recurrent herpesvirus infection.
Gu
M
M
Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo 14214, USA.
Haraszthy
G G
GG
Collins
A R
AR
Bergey
E J
EJ
D000818:Animals; D000998:Antiviral Agents / Q000502:physiology*; D002522:Chlorocebus aethiops; D015891:Cystatins / Q000032:analysis / Q000494:pharmacology / Q000502:physiology; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D010455:Peptides / Q000032:analysis / Q000494:pharmacology / Q000502:physiology; D055232:Proline-Rich Protein Domains; D011485:Protein Binding; D012471:Salivary Proteins and Peptides / Q000032:analysis* / Q000494:pharmacology / Q000502:physiology*; D014709:Vero Cells; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects*
D000998:Antiviral Agents; D015891:Cystatins; D010455:Peptides; D012471:Salivary Proteins and Peptides; D014764:Viral Proteins