Involvement of p59fynT in interleukin-5 receptor signaling.
Previous studies implicate the nonreceptor protein tyrosine kinase (PTK) p59fyn in the propagation of signals from the B cell antigen receptor. To elucidate the functions of this kinase, we examined B cell responsiveness in mice engineered to lack the hematopoietic isoform of p59fyn. Remarkably, antigen receptor signaling was only modestly defective in fynTnull B cells. In contrast, signaling from the interleukin (IL)-5 receptor which ordinarily provides a comitogenic stimulus with antiimmunoglobulin, was completely blocked. Our results document the importance of p59fynT in IL-5 responses in B cells, and they support a general model for cytokine receptor signal transduction involving the simultaneous recruitment of at least three families of PTK.
Appleby
M W
MW
Howard Hughes Medical Institute, Seattle, Washington 98195, USA.
Kerner
J D
JD
Chien
S
S
Maliszewski
C R
CR
Bondada
S
S
Perlmutter
R M
RM
Bondadaa S [corrected to Bondada
S ]
S]
D000818:Animals; D000941:Antigens / Q000276:immunology; D000955:Antigens, T-Independent / Q000276:immunology; D001402:B-Lymphocytes / Q000201:enzymology / Q000276:immunology*; D005260:Female; D007114:Immunization; D015848:Interleukin-5 / Q000502:physiology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D011518:Proto-Oncogene Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D051076:Proto-Oncogene Proteins c-fyn; D011947:Receptors, Antigen, B-Cell / Q000502:physiology*; D018123:Receptors, Interleukin / Q000235:genetics / Q000502:physiology*; D053649:Receptors, Interleukin-5; D015398:Signal Transduction / Q000502:physiology*
D000941:Antigens; D000955:Antigens, T-Independent; D015848:Interleukin-5; D011518:Proto-Oncogene Proteins; D011947:Receptors, Antigen, B-Cell; D018123:Receptors, Interleukin; D053649:Receptors, Interleukin-5; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn