Expression of mRNA for atrial natriuretic peptide receptor-A in human liver: detection using RT-PCR.
cDNA preparations obtained from human liver poly A+ RNA by reverse transcription were subjected to polymerase chain reaction. Primers used in the reaction were designed from the reported cDNA sequence for human placental atrial natriuretic peptide receptor-A (ANPRA). Sequence analysis of the amplified product (approximately 700 bp) showed 100% identity to the 2476-3189 bp region of the reported cDNA sequence of human placental ANPRA. Northern blot analysis of human liver poly A+ RNA fractions showed a hybridization signal at approximately 4.4 kb, identical to the signal obtained from the poly A+ RNA fractions of human placenta. These results indicate the possible mRNA expression for atrial natriuretic peptide receptor-A in human liver.
Pardhasaradhi
K
K
Section on Drug-Tissue Interaction, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
Kutty
R K
RK
Bertolotti
R
R
Krishna
G
G
D009320:Atrial Natriuretic Factor / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D008099:Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D012333:RNA, Messenger; D009320:Atrial Natriuretic Factor