title: The gene encoding the biotin-apoprotein ligase of Saccharomyces cerevisiae.
abstract: We report the isolation, genomic mapping, and DNA sequence of the BPL1 gene encoding the biotin-apoprotein ligase of Saccharomyces cerevisiae. The gene was isolated by complementation of an Escherichia coli birA (biotin-apoprotein ligase) mutant indicating that the expressed yeast protein modified the essential biotinated protein of the bacterial host. The BPL1 gene encodes a protein of 690 residues (M(r) 76.4 kDa) with strong sequence similarities to the E. coli and human biotin-apoprotein ligases. BPL1 was mapped to chromosome IV, is allelic to the previously described ACC2 gene, and encodes the major (if not the only) biotin-apoprotein ligase activity of S. cerevisiae.
authors.affiliation: Department of Microbiology, University of Illinois, Urbana 6180, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Cronan
authors.forename: J C
authors.initials: JC
authors.lastname: Wallace
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D001059:Apoproteins / Q000378:metabolism*; D001483:Base Sequence; D001710:Biotin / Q000378:metabolism*; D002874:Chromosome Mapping; D005800:Genes, Fungal; D008025:Ligases / Q000235:genetics*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics
chemical_list: D001059:Apoproteins; D001710:Biotin; D008025:Ligases