Effects of magnesium and iron on lipid peroxidation in cultured hepatocytes.
In primary cultures of rat hepatocytes, the effects of extracellular Mg2+ and Fe on lipid peroxidation (LPO) as measured by means of malondialdehyde (MDA) formation were investigated. Incubation of hepatocytes at decreasing extracellular Mg2+ concentration enhanced LPO, depending on extracellular Fe. About 96% of MDA accumulated in the culture medium. Addition of desferrioxamine prevented LPO. Additionally, the formation of oxygen free radicals was determined by fluorescence reduction of cis-parinaric acid. With this method, an immediate decay of fluorescence was found after addition of Fe2+. Fluorescence reduction was completely prevented by desferrioxamine, indicating the function of extracellular Fe. This mechanism may operate additionally to the increase in intracellular Fe and intracellular formation of oxygen free radicals during Mg deficiency in vivo.
Günther
T
T
Institute of Molecular Biology and Biochemistry, Free University of Berlin, Germany.
Vormann
J
J
Höllriegl
V
V
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D003470:Culture Media; D003676:Deferoxamine / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005296:Ferrous Compounds / Q000378:metabolism / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism*; D051381:Rats; D017382:Reactive Oxygen Species / Q000494:pharmacology; D013997:Time Factors
D003470:Culture Media; D005231:Fatty Acids, Unsaturated; D005296:Ferrous Compounds; D005609:Free Radicals; D017382:Reactive Oxygen Species; C020748:ferrous sulfate; D008315:Malondialdehyde; D008274:Magnesium; D003676:Deferoxamine; C013641:parinaric acid; D001205:Ascorbic Acid