title: Benzo[a]pyrene-induced alterations in total immune cell number and cell-surface antigen expression in the thymus, spleen and bone marrow of B6C3F1 mice.
abstract: Although considerable evidence suggests that carcinogenic polycyclic aromatic hydrocarbons are immunosuppressive compounds, limited information is available regarding precise effects of these agents on immune cells. In the present report, the effect of subchronic exposure to benzo[a]pyrene (B[a]P) on immune cells in bone marrow, thymus and spleen of B6C3F1 mice was investigated. B[a]P treatment was found to reduce thymic cellularity and also to significantly alter normal thymocyte differentiation, as indicated by expression of CD4 and CD8 cell-surface antigens. Exposure to B[a]P also reduced cellularity of the bone marrow, including decreased percentage and absolute number of CD45R+ B-lineage lymphocytes. Further, B[a]P treatment dramatically increased the percentage of CD44hi cells in bone marrow, while proportionately reducing CD44lo cells. These results may indicate CD44lo bone marrow cells, including prolymphocytic cells, represent sensitive targets of B[a]P. The spleens of treated mice were found deficient in both Thy 1.2+ T lymphocytes and CD45R+ B lymphocytes, effects that correlate well with the chemical-induced alterations observed in thymus and bone marrow, respectively.
authors.affiliation: Department of Biomedical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg 24061-0442, USA.
authors.forename: S D
authors.initials: SD
authors.lastname: Holladay
authors.forename: B J
authors.initials: BJ
authors.lastname: Smith
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000096:biosynthesis*; D001564:Benzo(a)pyrene / Q000494:pharmacology*; D001853:Bone Marrow / Q000187:drug effects / Q000276:immunology; D001854:Bone Marrow Cells; D005260:Female; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000187:drug effects* / Q000276:immunology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D051379:Mice; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology / Q000187:drug effects / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D007166:Immunosuppressive Agents; D001564:Benzo(a)pyrene