title: Distribution of beta 3-adrenoceptor mRNA in human tissues.
abstract: The beta 3-adrenoceptor is a G protein-coupled receptor which mediates metabolic functions of the endogenous catecholamines epinephrine and norepinephrine. Questions exist regarding distribution of the beta 3-adrenoceptor in human tissue. In order to examine the distribution of beta 3-adrenoceptor mRNA in human tissues, we used sensitive and specific RNase protection assays without previous PCR amplification in an extensive list of human tissues. We confirm the presence of beta 3-adrenoceptor mRNA in human white fat from several locations, gall bladder, and small intestine, as well as extend the distribution of beta 3-adrenoceptor mRNA to previously uncharacterized human tissues such as stomach and prostate. The presence of beta 3-adrenoceptor mRNA in human white adipose tissue has important implications regarding possible use of beta 3-adrenoceptor selective agonists as anti-obesity agents, and the demonstration of beta 3-adrenoceptor mRNA in a number of gastrointestinal tissues and prostate raises the question of the role of the beta 3-adrenoceptor in motility and secretory processes.
authors.affiliation: Department of Anesthesiology, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: D E
authors.initials: DE
authors.lastname: Berkowitz
authors.forename: N A
authors.initials: NA
authors.lastname: Nardone
authors.forename: R M
authors.initials: RM
authors.lastname: Smiley
authors.forename: D T
authors.initials: DT
authors.lastname: Price
authors.forename: D K
authors.initials: DK
authors.lastname: Kreutter
authors.forename: R T
authors.initials: RT
authors.lastname: Fremeau
authors.forename: D A
authors.initials: DA
authors.lastname: Schwinn
mesh_terms: D000818:Animals; D001345:Autoradiography; D016466:CHO Cells; D002460:Cell Line; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism; D006224:Cricetinae; D018076:DNA, Complementary; D005704:Gallbladder / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta