Assessment of viability and proliferation of in vivo silicone-primed lymphocytes after in vitro re-exposure to silicone.
The functional response of peripheral blood lymphocytes isolated from 22 patients with silicone gel-filled breast implants was assessed after in vitro re-exposure to silicone. Using cell culture test methods to quantify proliferation and viability and/or activation of lymphocyte microcultures, i.e., the uptake of tritiated thymidine (3H-TdR uptake test) and the reduction of formazan salts (MTT assay), interesting data were obtained. Peripheral blood lymphocytes purified from patients wearing silicone gel-filled breast implants react in vitro to silicone showing a statistically significant increase of both proliferation and viability, while healthy subjects do not respond on in vitro exposure to silicone. Differences resulted even more statistically significant when patients were divided into two groups depending on the type of surgery they underwent: patients with breast augmentation for aesthetic reasons seem to have an increased responsiveness in vitro to silicone compared to patients who experienced a reconstructive surgery of the breast. Although they are still preliminary, being referred to a limited population, these results suggest that the lymphocytes of patients with silicone gel-filled breast implants could be sensitized in vivo toward silicone; the re-exposure of these cells to silicone leads to a higher functional response which could be looked for by using quantitative in vitro test methods.
Ciapetti
G
G
Laboratory for Biocompatibility Research on Implant Materials, Istituti Ortopedici Rizzoli, Bologna, Italy.
Granchi
D
D
Stea
S
S
Cenni
E
E
Schiavon
P
P
Giuliani
R
R
Pizzoferrato
A
A
D000328:Adult; D001940:Breast / Q000166:cytology; D018427:Breast Implants; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000494:pharmacology; D005260:Female; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000187:drug effects / Q000502:physiology*; D008875:Middle Aged; D012828:Silicones / Q000494:pharmacology*; D013778:Tetrazolium Salts
D004129:Dimethylpolysiloxanes; D012828:Silicones; D013778:Tetrazolium Salts; C042207:4-anisyltetrazolium blue