title: Evaluation of the Etest for penicillin susceptibility testing of Neisseria meningitidis.
abstract: Susceptibility to penicillin of 30 strains (one isolate per patient) of Neisseria meningitidis isolated from blood (N = 19) or cerebrospinal fluid (N = 11) was studied by two methods. Minimum inhibitory concentrations (MICs) obtained with the Etest were compared to those obtained by the National Committee for Clinical Laboratory Standards agar dilution method. Twenty meningococci (67%) relatively resistant to penicillin were identified by both methods. The mean MIC from the reference method was 0.32 micrograms/ml (range, 0.2-1) and by the Etest method was 0.35 micrograms/ml (range, 0.19-1.25). All MICs obtained by the Etest method were within one dilution of the MICs obtained by the reference method. Because of the increase in penicillin MIC of meningococcal isolates in Spain, we evaluated the performance of the Etest as an alternative method for penicillin-susceptibility testing of N. meningitidis. The Etest is a simple and accurate method for determining the susceptibility of N. meningitidis to penicillin.
authors.affiliation: Department of Microbiology, Asturias University Hospital, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Gomez-Herruz
authors.forename: R
authors.initials: R
authors.lastname: González-Palacios
authors.forename: J
authors.initials: J
authors.lastname: Romanyk
authors.forename: J A
authors.initials: JA
authors.lastname: Cuadros
authors.forename: J
authors.initials: J
authors.lastname: Ena
mesh_terms: D015169:Colony Count, Microbial; D005069:Evaluation Studies as Topic; D008826:Microbial Sensitivity Tests; D009345:Neisseria meningitidis / Q000145:classification / Q000187:drug effects*; D010403:Penicillin Resistance; D010406:Penicillins / Q000494:pharmacology*
chemical_list: D010406:Penicillins