title: The membrane proximal region of the cytoplasmic domain of the growth hormone receptor is involved in the activation of Stat 3.
abstract: Growth hormone receptor (GHR) signaling involves activation of the Janus Kinases (Jak) and of Stat proteins (signal transducers and activators of transcription). Growth hormone (GH) induces transcriptional activation of c-fos gene and the c-sis inducible element (SIE) of its promoter was shown to bind the Stat proteins. Using cells co-transfected with GHR and Stat 3 expression vectors, we directly demonstrate that GH induces tyrosine phosphorylation of Stat 3 and its binding to the SIE probe. We showed, using mutant forms of GHR, that only the cytoplasmic membrane proximal domain of the receptor, including a conserved proline rich region (box 1), is required for this effect.
authors.affiliation: INSERM U344, Faculté de Médecine Necker, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Sotiropoulos
authors.forename: S
authors.initials: S
authors.lastname: Moutoussamy
authors.forename: N
authors.initials: N
authors.lastname: Binart
authors.forename: P A
authors.initials: PA
authors.lastname: Kelly
authors.forename: J
authors.initials: J
authors.lastname: Finidori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D006224:Cricetinae; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D015342:DNA Probes; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D013006:Growth Hormone / Q000494:pharmacology; D007700:Kinetics; D008969:Molecular Sequence Data; D010766:Phosphorylation / Q000187:drug effects; D011401:Promoter Regions, Genetic / Q000235:genetics; D011986:Receptors, Somatotropin / Q000235:genetics / Q000378:metabolism*; D050796:STAT3 Transcription Factor; D017384:Sequence Deletion; D015398:Signal Transduction / Q000502:physiology; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation / Q000502:physiology*; D014162:Transfection
chemical_list: D015342:DNA Probes; D004268:DNA-Binding Proteins; D011986:Receptors, Somatotropin; D050796:STAT3 Transcription Factor; D015534:Trans-Activators; D013006:Growth Hormone; D004247:DNA