title: Effect of dopamine receptor blockade on the renal excretory response to acute volume expansion (AVE) in conscious Brattleboro rats.
abstract: All the studies were performed on chronically cannulated, conscious Brattleboro rats, housed in metabolic cages under standard conditions. The renal excretory indices: diuresis (V), sodium (UNa.V), potassium (UK.V), chloride (UCl.V) and osmotic (Uosm.V) excretion were measured under the following experimental protocol: (1) controls, (2) 2% volume expansion (AVE)-0.9% NaCl i.v. for 2 min, (3) Dopamine (DA) receptor antagonist Flupentixol (FLU) 0.5 mg.kg-1 i.v., and (4) AVE 10 min after i.v. application of FLU. All the indices studied, given as cumulative curves, showed a firm linearity with time during the whole experimental period (5 h) in groups 1 and 2 (p < 0.05), while such a relationship was not found significantly in groups 3 and 4. AVE quickly, strongly and significantly (p < 0.01) increased V and especially UNa.V, UCl.V and Uosm.V. Pretreatment with FLU potently suppressed the effect of AVE on the renal excretion. FLU alone decreased almost equally V and saliuresis.
  
  
  CONCLUSIONS
  the DA antagonist FLU blocks the diuretic and saliuretic response to AVE probably by affecting tubular processes.
authors.affiliation: Department of Physiology, Medical Faculty, Sofia, Bulgaria.
authors.forename: N
authors.initials: N
authors.lastname: Tzaneva
authors.forename: N
authors.initials: N
authors.lastname: Belova
authors.forename: L
authors.initials: L
authors.lastname: Kirkova
authors.forename: B
authors.initials: B
authors.lastname: Piryova
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D001810:Blood Volume / Q000502:physiology*; D002712:Chlorides / Q000652:urine; D018492:Dopamine Antagonists / Q000494:pharmacology*; D005475:Flupenthixol / Q000494:pharmacology; D006339:Heart Rate / Q000502:physiology; D007668:Kidney / Q000378:metabolism*; D008297:Male; D009994:Osmolar Concentration; D011188:Potassium / Q000652:urine; D051381:Rats; D011910:Rats, Brattleboro; D012964:Sodium / Q000652:urine; D014563:Urodynamics / Q000502:physiology
chemical_list: D002712:Chlorides; D018492:Dopamine Antagonists; D012964:Sodium; D005475:Flupenthixol; D011188:Potassium