A monoclonal antibody to an ovine gp130 molecule inhibits homotypic aggregation induced by anti-CD43 monoclonal antibodies of ruminant leukocytes.
A monoclonal antibody (mAb) Co.3E1D4 which recognized a molecule present on the surface of ovine peripheral blood granulocytes, monocytes, platelets and a small percentage of lymphocytes, was able to block leukocyte aggregation induced by mAbs specific for the leukosialin or CD43 molecule. Co.3E1D4 precipitated an antigen with apparent molecular weight of 130 kDa (under reducing and non-reducing conditions). Endoglycosidase F digestion of the molecule resulted in reduction in the apparent molecular weight. These data suggest that the antigen recognized by Co.3E1D4 may be homologous to the human CD31 or platelet endothelial cell adhesion molecule-1 (PECAM-1). Aggregation produced by other inducers such as PMA was not blocked showing that only some cell-cell interactions were mediated by the gp130 molecule.
Pintado
C O
CO
Departamento de Genética, Facultad de Veterinaria, Universidad de Córdoba, Spain.
Perez de la Lastra
J
J
Llanes
D
D
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic / Q000502:physiology; D002417:Cattle / Q000097:blood / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000502:physiology; D002449:Cell Aggregation / Q000187:drug effects; D006041:Goats / Q000097:blood / Q000276:immunology; D006736:Horses / Q000097:blood / Q000276:immunology; D006801:Humans; D051917:Leukosialin; D008562:Membrane Glycoproteins / Q000276:immunology* / Q000502:physiology; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D012418:Ruminants / Q000097:blood / Q000276:immunology*; D012756:Sheep / Q000097:blood / Q000276:immunology; D012795:Sialoglycoproteins / Q000276:immunology*; D013045:Species Specificity; D013552:Swine / Q000097:blood / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015815:Cell Adhesion Molecules; D051917:Leukosialin; D008562:Membrane Glycoproteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C465014:SPN protein, human; D012795:Sialoglycoproteins