title: The limbic system-associated membrane protein is an Ig superfamily member that mediates selective neuronal growth and axon targeting.
abstract: The formation of brain circuits requires molecular recognition between functionally related neurons. We report the cloning of a molecule that participates in these interactions. The limbic system-associated membrane protein (LAMP) is an immunoglobulin (Ig) superfamily member with 3 Ig domains and a glycosyl-phosphatidylinositol anchor. In the developing forebrain, lamp is expressed mostly by neurons comprising limbic-associated cortical and subcortical regions that function in cognition, emotion, memory, and learning. The unique distribution of LAMP reflects its functional specificity. LAMP-transfected cells selectively facilitate neurite outgrowth of primary limbic neurons. Most striking, administration of anti-LAMP in vivo results in abnormal growth of the mossy fiber projection from developing granule neurons in the dentate gyrus of the hippocampal formation, suggesting that LAMP is essential for proper targeting of this pathway. Rather than being a general guidance cue, LAMP likely serves as a recognition molecule for the formation of limbic connections.
authors.affiliation: Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854, USA.
authors.forename: A F
authors.initials: AF
authors.lastname: Pimenta
authors.forename: V
authors.initials: V
authors.lastname: Zhukareva
authors.forename: M F
authors.initials: MF
authors.lastname: Barbe
authors.forename: B S
authors.initials: BS
authors.lastname: Reinoso
authors.forename: C
authors.initials: C
authors.lastname: Grimley
authors.forename: W
authors.initials: W
authors.lastname: Henzel
authors.forename: I
authors.initials: I
authors.lastname: Fischer
authors.forename: P
authors.initials: P
authors.lastname: Levitt
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001369:Axons; D016466:CHO Cells; D002448:Cell Adhesion; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006224:Cricetinae; D003412:Cricetulus; D058851:GPI-Linked Proteins; D005796:Genes; D017261:Glycosylphosphatidylinositols; D006624:Hippocampus / Q000737:chemistry / Q000254:growth & development / Q000648:ultrastructure; D008032:Limbic System / Q000737:chemistry* / Q000196:embryology / Q000254:growth & development / Q000648:ultrastructure; D008969:Molecular Sequence Data; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D009434:Neural Pathways; D016366:Open Reading Frames; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014162:Transfection
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D009419:Nerve Tissue Proteins; D011993:Recombinant Fusion Proteins; C095362:limbic system-associated membrane protein