title: Overproduced and purified receptor binding protein pb5 of bacteriophage T5 binds to the T5 receptor protein FhuA.
abstract: A promotor-less oad gene of bacteriophage T5, encoding the receptor binding protein pb5, was cloned into pT7-3 under the control of phage T7 promoter phi 10. Induction with IPTG resulted in enhanced production of pb5. Upon fractionation of the producing cells, most of the overproduced pb5 was found in the membrane fraction, which was most likely due to aggregation of the protein. The minor, soluble fraction of pb5 specifically inhibited adsorption of T5 to its FhuA receptor protein. Inhibition was also seen with trace amounts of pb5, and binding of pb5 to FhuA appeared to be almost irreversible. Purification of pb5 from the cytosolic fraction was performed by FPLC using a MonoQ column. pb5, which did not bind to the matrix of the column, was obtained in almost pure form. The purified protein also inhibited T5 adsorption.
authors.affiliation: Universität Konstanz, Fakultät für Biologie, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Mondigler
authors.forename: R T
authors.initials: RT
authors.lastname: Vögele
authors.forename: K J
authors.initials: KJ
authors.lastname: Heller
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D029968:Escherichia coli Proteins; D011991:Receptors, Virus / Q000378:metabolism*; D013604:T-Phages / Q000502:physiology*; D014764:Viral Proteins / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029968:Escherichia coli Proteins; C050900:FhuA protein, E coli; D011991:Receptors, Virus; D014764:Viral Proteins; C067129:oad protein, Bacteriophage T5