Purification and characterization of Eikenella corrodens type IV pilin.
Eikenella corrodens is a gram-negative human pathogen associated with periodontal diseases and soft-tissue infections. Pilin was purified by association-dissociation and fast protein liquid chromatography; it had an apparent molecular mass of about 14.8 kDa and an N-terminal amino acid sequence reflective of type IV pilins. Antibodies to the purified protein reacted with pili on whole cells. This is the first report of purification of type IV pili/pilin from this organism. Other type IV pili are important virulence factors; we are currently investigating the biological role of pili in E. corrodens.
Hood
B L
BL
School of Biological Sciences, University of Missouri-Kansas City 64110, USA.
Hirschberg
R
R
D000595:Amino Acid Sequence; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D016124:Eikenella corrodens / Q000737:chemistry* / Q000648:ultrastructure; D036781:Fimbriae Proteins; D008969:Molecular Sequence Data; D011817:Rabbits
D001425:Bacterial Outer Membrane Proteins; D036781:Fimbriae Proteins