In vivo activity of the hepatitis B virus core promoter: tissue specificity and temporal regulation.
The contribution of the hepatitis B virus enhancers I and II in the regulation of the activity of the core and the X promoters was assessed in transgenic mice. Surprisingly, despite the presence of heterologous promoters linked 5' of the X gene, the transgene expression is mostly due to core promoter (Cp) activity present in the X coding sequence. Moreover, the restriction of Cp activity to hepatic tissue required the combined action of both enhancers I and II, whereas the proximity of these two enhancers was insufficient to confer tissue specificity on Xp activity. Furthermore, the liver-specific activity of the Cp was developmentally regulated in an enhancer I-independent manner.
Billet
O
O
Institut National de la Santé et de la Recherche Médicale U-380, Institut Cochin de Génétique Moléculaire, Paris, France.
Grimber
G
G
Levrero
M
M
Seye
K A
KA
Briand
P
P
Joulin
V
V
D000818:Animals; D015152:Blotting, Northern; D004622:Embryo, Mammalian; D004742:Enhancer Elements, Genetic; D015967:Gene Expression Regulation, Viral; D005814:Genes, Viral; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis* / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D015183:Restriction Mapping; D016662:Virus Integration
D006512:Hepatitis B Core Antigens; D012367:RNA, Viral