Effects of an antibiotic protease inhibitor, actinonin on the growth within collagen gels of non-metastatic and metastatic mouse mammary tumors of the same origin.
Of five autonomous sublines established independently from the transplantable hormone-dependent mouse mammary tumor, TPDMT-4, three but not two acquired metastatic potential. In in vitro culture using collagen gels, actinonin, an antibiotic protease inhibitor exerted a stronger growth-inhibiting effect on the metastatic than on the parent and non-metastatic tumors. Zymographic analysis demonstrated the active forms of gelatinases in the metastatic but not in the non-metastatic sublines and the complete inhibition of the enzyme activities by actinonin. Gelatinases/type IV collagenases might play an important role in tumor progression towards metastatic phenotype and actinonin may suppress tumor growth through inhibiting collagenase.
Sayama
K
K
Department of Animal Science, Faculty of Agriculture, Shizuoka University, Japan.
Goto
Y
Y
Iguchi
T
T
Takeda
Y
Y
Matsuzawa
A
A
D000818:Animals; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D018093:Gelatinases / Q000378:metabolism*; D006877:Hydroxamic Acids / Q000494:pharmacology; D015674:Mammary Neoplasms, Animal / Q000201:enzymology* / Q000473:pathology*; D051379:Mice
D006877:Hydroxamic Acids; D018093:Gelatinases; C010531:actinonin