title: Effects of cycloheximide on chromatin-bound and -unbound PCNA in HeLa cells.
abstract: We examined the effects of cycloheximide on the amount of chromatin-bound and -unbound PCNA subpopulations in HeLa cells. The chromatin-bound PCNA content decreased rapidly in the presence of cycloheximide, whereas the reduction of the total amount of PCNA was minimal even 24 h after the treatment with cycloheximide. From these data, the half-life of PCNA was estimated to be 31.0 h for chromatin-bound PCNA and 276.8 h for the total.
authors.affiliation: Department of Pathology, Iwate Medical University, Morioka, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sasaki
authors.forename: A
authors.initials: A
authors.lastname: Kurose
authors.forename: N
authors.initials: N
authors.lastname: Uesugi
authors.forename: A
authors.initials: A
authors.lastname: Kato
mesh_terms: D002843:Chromatin / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology*; D006207:Half-Life; D006367:HeLa Cells / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D018809:Proliferating Cell Nuclear Antigen / Q000187:drug effects* / Q000378:metabolism
chemical_list: D002843:Chromatin; D018809:Proliferating Cell Nuclear Antigen; D003513:Cycloheximide