A unique endoglucanase-encoding gene cloned from the phytopathogenic fungus Macrophomina phaseolina.
The deduced amino acid sequence derived from a Macrophomina phaseolina beta-1,4-endoglucanase-encoding gene revealed 48% identity (over 119 amino acids) with egl1 from the phytopathogen Pseudomonas solanacearum. Its similarity to saprophyte endoglucanases was not significant. Its minimum substrate size, unlike that of any known saprophyte endoglucanase, was cellopentaose. The unique characteristics of M. phaseolina egl1-encoded endoglucanase suggest that it is phytopathogen specific.
Wang
H
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Department of Botany & Plant Pathology, Purdue University, West Lafayette, Indiana 47907, USA.
Jones
R W
RW
D000595:Amino Acid Sequence; D001483:Base Sequence; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005685:Galactans / Q000378:metabolism; D005800:Genes, Fungal; D005936:Glucans / Q000378:metabolism; D003904:Mitosporic Fungi / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D014990:Xylans / Q000378:metabolism
D018076:DNA, Complementary; D005656:Fungal Proteins; D005685:Galactans; D005936:Glucans; D011134:Polysaccharides; D014990:Xylans; C008247:laminaran; D002480:Cellulase; C005653:arabinogalactan