title: Differential localization of heme oxygenase and NADPH-diaphorase in spinal cord neurons.
abstract: Western blot analysis using several antibodies showed that rat spinal cord contained abundant immunostainable heme oxygenase-2 (HO-2) and barely detectable levels of heme oxygenase-1 (HO-1). Anti-HO-2 antibody stained large anterior horn motoneurones and numerous smaller neurons throughout spinal cord gray matter including the dorsal root entry zone. HO-2+ astrocytes were not evident in gray matter although their presence cannot be ruled out. The distribution of HO-2+ neurons was compared with the distribution of cells containing NADPH-diaphorase (NADPH-d) activity, a marker for nitric oxide synthase. NADPH-d activity was restricted to far fewer neurons, many of which were close to the central canal and dorsal root entry zone.
authors.affiliation: Molecular Neurobiology Laboratory (IIIN-1), Department of Veterans Affairs Medical Center, Sepulveda, CA 91343, USA.
authors.forename: B E
authors.initials: BE
authors.lastname: Dwyer
authors.forename: R N
authors.initials: RN
authors.lastname: Nishimura
authors.forename: S Y
authors.initials: SY
authors.lastname: Lu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D006360:Heat-Shock Proteins / Q000378:metabolism; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism*; D007150:Immunohistochemistry; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000201:enzymology*; D051381:Rats; D013116:Spinal Cord / Q000166:cytology* / Q000201:enzymology*; D016707:Tissue Fixation
chemical_list: D006360:Heat-Shock Proteins; D006419:Heme Oxygenase (Decyclizing); D009252:NADPH Dehydrogenase