Cytokinetic studies of oral cancer cells using bromodeoxyuridine labelling in relation to factors influencing prognosis.
Cytokinetics of 27 untreated oral squamous cell carcinomas were evaluated by in vitro bromodeoxyuridine (BrdU) labelling. A mononuclear cell suspension was prepared for the labelling using collagenase and DNase treatment. The labelled cells were visualised by immunofluorescence staining with an anti-BrdU monoclonal antibody. The labelling index (LI%) was calculated by determining the percentage of BrdU labelled cells. The LIs of the carcinomas ranged from 3.8 to 19.2%. The LIs of poorly-differentiated (HG3) tumours were 2-3-fold higher compared with those of well-differentiated (HG1) and moderately-differentiated (HG2) carcinomas. Results also showed statistically significant (P < 0.0005) increases in LIs from nuclear grade 1 (NG1) to nuclear grade 3 (NG3) carcinomas. Higher LIs were observed in stage III/IV (10.82 +/- 4.62; 10.36 +/- 4.90) than those in stage I (6.87 +/- 2.09) and II (7.14 +/- 1.87) carcinomas. A significant (P < 0.0005) difference in LI values was found between the patients with positive and negative lymph nodes. Good correlation (r = 0.77) was exhibited between the LI values and mitotic counts (MC) of the specimens. These results on oral cancer cell proliferation seem to have prognostic implications.
Mukhopadhyay
D
D
Department of Tumour Virology, Chittaranjan National Cancer Institute, Calcutta, India.
Chatterjee
R
R
Chakraborty
R N
RN
D000328:Adult; D000368:Aged; D001973:Bromodeoxyuridine; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002453:Cell Cycle; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D008940:Mitotic Index; D009062:Mouth Neoplasms / Q000473:pathology*; D011379:Prognosis
D001973:Bromodeoxyuridine