title: [Changes in the activity of endonucleolysis of nuclear DNA in children with acute leukemia].
abstract: The previously established fact of low activity of Ca, Mg-dependent endonucleolysis of cell nucleus DNA in lymphoproliferative diseases (CME-activity) brought the authors to study intranuclear characteristics of lymphoid cells in childhood acute lymphoblastic leukemia (ALL). The intensity of DNA-endonucleolysis was measured in 0.7% agarose gel using electrophoresis. Peripheral blood and bone marrow samples from 13 untreated ALL patients and 23 children in remission were examined. The age of the patients ranged from 4 to 14 years. CME-activity before treatment appeared to be 2-10 times less than normal in 8 out of 13 patients. In bone marrow cell nuclei CME-activity was universally reduced 3-20-fold. In ALL remission endonuclease activity in blood and bone marrow cells returned to normal.
authors.forename: S S
authors.initials: SS
authors.lastname: Kirzon
authors.forename: R V
authors.initials: RV
authors.lastname: Lenskaia
authors.forename: T I
authors.initials: TI
authors.lastname: Tkacheva
authors.forename: E N
authors.initials: EN
authors.lastname: Aref'eva
authors.forename: Ia Ia
authors.initials: IaIa
authors.lastname: Lutskaia
authors.forename: E A
authors.initials: EA
authors.lastname: Mamedova
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D004273:DNA, Neoplasm / Q000235:genetics*; D004720:Endonucleases / Q000378:metabolism*; D006801:Humans; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D004720:Endonucleases
vernacular_title: Izmenenie aktivnosti éndonukleoza DNK-kletochnykh iader pri ostrom leĭkoze u deteĭ.