title: Complete sequence of a 38.4-kb human cosmid insert containing the polymorphic marker DXS455 from Xq28.
abstract: The complete DNA sequence of a cosmid mapping to human Xq28 (DXS455) has been determined using a shotgun approach (genbank accession number: L31948). The cosmid insert is 38.4 kb in length, and contains several repetitive sequences, including a highly repetitive region located in a fragment previously shown to contain a polymorphic VNTR locus, as well as short di- and tri-nucleotide repeats. In addition to the previously known VNTR locus, a CA-repeat and an unusual 800-bp repetitive region have been found to be polymorphic. The repeated sequence mapping to the site of the VNTR locus spans 480 bp in the cosmid. In addition to several AT-dinucleotide stretches, this repeat has four copies of a 25 bp repeat unit and flanking sequences similar to this 25-mer. No expressed sequences have so far been identified in this cosmid.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: B
authors.initials: B
authors.lastname: Andersson
authors.forename: F
authors.initials: F
authors.lastname: Lu
authors.forename: D M
authors.initials: DM
authors.lastname: Muzny
authors.forename: S T
authors.initials: ST
authors.lastname: Warren
authors.forename: R A
authors.initials: RA
authors.lastname: Gibbs
mesh_terms: D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D003360:Cosmids / Q000235:genetics*; D004247:DNA / Q000235:genetics; D015723:Gene Library; D005819:Genetic Markers; D006801:Humans; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid; D014960:X Chromosome
chemical_list: D005819:Genetic Markers; D004247:DNA