Rapid high-performance liquid chromatographic method for the quantitation of polyamines as their dansyl derivatives: application to plant and animal tissues.
A rapid high-performance liquid chromatographic method for the separation of polyamines as their dansyl derivative has been developed. The chromatographic system used consisted of a reversed-phase column and a mobile phase of acetonitrile and water. The separation of 1,3-diaminopropane, putrescine, cadaverine, spermidine and spermine takes only 9 min. This method provides a good resolution between 1,3-diaminopropane and putrescine. It has been applied to quantify polyamines from seeds of wheat, petals of Phalaenopsis hybrids and various rat tissues.
Marcé
M
M
Laboratori de Fisiologia Vegetal, Facultat de Farmàcia, Universitat de Barcelona, Spain.
Brown
D S
DS
Capell
T
T
Figueras
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X
Tiburcio
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AF
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003619:Dansyl Compounds / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D011073:Polyamines / Q000032:analysis*; D051381:Rats; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D003619:Dansyl Compounds; D011073:Polyamines