Testing for antibody to human immunodeficiency virus type 1 in a population in which mycobacterial diseases are endemic.
During a large epidemiologic study in the Karonga District of northern Malawi, serum samples from 139 patients with incident leprosy, 124 with newly diagnosed leprosy, 277 patients with incident tuberculosis, and 2296 controls were tested for antibodies to human immunodeficiency virus. Sera were tested according to a four-test protocol using two ELISAs and two particle agglutination assays. Overall, 188 samples were considered positive, 2634 were considered negative, and 14 were indeterminate. All 18 available positive specimens from leprosy patients, a random sample of 14 positive specimens from tuberculosis patients, and 15 positive specimens from controls were tested by Western blot. There was no evidence of substantial numbers of ELISA false-positives in any patient group or among controls.
Sterne
J A
JA
Communicable Disease Epidemiology Unit, London School of Hygiene and Tropical Medicine, United Kingdom.
Turner
A C
AC
Fine
P E
PE
Parry
J V
JV
Lucas
S B
SB
Pönnighaus
J M
JM
Mkandwire
P K
PK
Nyasulu
S
S
Warndorff
D K
DK
D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D016022:Case-Control Studies; D005189:False Positive Reactions; D005260:Female; D018023:HIV Seronegativity; D006679:HIV Seropositivity / Q000175:diagnosis*; D015497:HIV-1 / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007918:Leprosy / Q000150:complications / Q000453:epidemiology; D008295:Malawi / Q000453:epidemiology; D008297:Male; D014376:Tuberculosis / Q000150:complications / Q000453:epidemiology*
D000914:Antibodies, Viral