title: The first nucleotide sequence of an archaeal elongation factor 1 beta gene.
abstract: An archaeal elongation factor 1 beta gene has been isolated for the first time from a Sulfolobus solfataricus genomic library. The sequenced clone (869 bp) contained two open reading frames, one coding for a protein made of 91 amino acid residues (SsEF-1 beta), the other one encoding a nonidentified product (ORF 115). The amino acid sequences of segments at the N- and C-terminal of the translated SsEF-1 beta were identical to those determined for the native protein. Northern and Southern analyses showed that the SsEF-1 beta gene is represented in S. solfataricus by a unique sequence. Compared to eubacterial or eukaryal corresponding genes the SsEF-1 beta is much shorter.
authors.affiliation: Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Arcari
authors.forename: G
authors.initials: G
authors.lastname: Raimo
authors.forename: G
authors.initials: G
authors.lastname: Ianniciello
authors.forename: M
authors.initials: M
authors.lastname: Gallo
authors.forename: V
authors.initials: V
authors.lastname: Bocchini
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000737:chemistry / Q000235:genetics*; D016944:Sulfolobus / Q000235:genetics*
chemical_list: D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors