title: Growth inhibition of leukaemic cells carrying the t(3;21) by the AML1/EVI-1-specific antisense oligonucleotide.
abstract: The t(3;21)(q26;q22) translocation is thought to play an important role in the acute phase transformation of CML. The formation of the AML1/EVI-1 fusion gene by the translocation leads to expression of the AML1/EVI-1 fusion protein. Here we demonstrate that the AML1/EVI-1-specific antisense oligonucleotide markedly decreases the [3H]thymidine incorporation and growth of leukaemic cells carrying the t(3;21) and the t(9;22), but not those of K562 cells. These results indicate that the AML1/EVI-1 fusion protein could contribute to proliferation of the t(3;21)-carrying leukaemic cells after entering the blastic crisis phase of CML.
authors.affiliation: Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Mitani
authors.forename: S
authors.initials: S
authors.lastname: Ogawa
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Kurokawa
authors.forename: Y
authors.initials: Y
authors.lastname: Yazaki
authors.forename: H
authors.initials: H
authors.lastname: Hirai
mesh_terms: D001483:Base Sequence; D002455:Cell Division; D002891:Chromosomes, Human, Pair 21; D002893:Chromosomes, Human, Pair 3; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense / Q000235:genetics*; D015514:Oncogene Proteins, Fusion; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
chemical_list: D016376:Oligonucleotides, Antisense; D015514:Oncogene Proteins, Fusion