title: NPY- and CGRP-like immunoreactive nerve fibers in the testis and mesorchium of the toad (Bufo arenarum).
abstract: The presence and distribution of peptidergic nerve fibers were studied in the testis and mesorchium of the toad by means of immunohistochemistry. Cryostat sections of the testis and whole-mount preparations of mesorchia were immunostained with antisera to calcitonin gene-related peptide (CGRP) and neuropeptide tyrosine (NPY). After leaving the mesorchium CGRP-immunoreactive (IR) fibers were seen predominantly running in between the seminiferous tubules. In addition, a small population of CGRP-IR nerve fibers formed thin plexuses around blood vessels. Conversely, NPY-like immunoreactivity predominated in nerve fibers that formed dense plexuses around vessels both in the mesorchium and testis. Additionally, some single NPY-IR nerve fibers could be seen in both structures studied. The functional significance of these peptidergic systems in the testis of the toad remains to be analyzed.
authors.affiliation: Instituto de Neurobiología, Buenos Aires, Argentina.
authors.forename: M V
authors.initials: MV
authors.lastname: Achi
authors.forename: J M
authors.initials: JM
authors.lastname: Figueroa
authors.forename: V
authors.initials: V
authors.lastname: González Nicolini
authors.forename: M J
authors.initials: MJ
authors.lastname: Villar
authors.forename: J H
authors.initials: JH
authors.lastname: Tramezzani
mesh_terms: D000818:Animals; D002022:Bufo arenarum; D015740:Calcitonin Gene-Related Peptide / Q000276:immunology / Q000378:metabolism*; D007668:Kidney / Q000294:innervation / Q000378:metabolism; D008297:Male; D008643:Mesentery / Q000294:innervation / Q000378:metabolism / Q000648:ultrastructure; D009412:Nerve Fibers / Q000378:metabolism* / Q000648:ultrastructure; D009478:Neuropeptide Y / Q000276:immunology / Q000378:metabolism*; D013737:Testis / Q000294:innervation* / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D009478:Neuropeptide Y; D015740:Calcitonin Gene-Related Peptide