Transforming growth factor-beta receptors on human endometrial cells: identification of the type I, II, and III receptors and glycosyl-phosphatidylinositol anchored TGF-beta binding proteins.
In the present study, we have characterized the cell surface receptors for transforming growth factor-beta (TGF-beta) on monolayer cultures of stromal cells prepared from human endometrial biopsies, and on a human endometrial epithelial cell line (RL95-2) using affinity cross-link labeling techniques. On the stromal cells, five TGF-beta binding proteins were identified. Analysis of the sensitivity of these proteins to dithiothreitol and phosphatidylinositol-specific phospholipase C, together with results from immunoprecipitations with antibodies against the type II and III TGF-beta receptors, confirmed that three of these binding proteins correspond to the cloned type I, II, and III TGF-beta receptors. The other two binding proteins observed exhibit the characteristics of isoform-specific GPI-anchored TGF-beta binding proteins. On RL95-2 cells, three TGF-beta binding proteins, corresponding to the type I, II, and III TGF-beta receptors, were identified. The receptors which we have characterized on endometrial cells are responsive to physiological concentrations of TGF-beta as demonstrated by the effect of TGF-beta on endometrial cell proliferation. Accordingly, these receptors have the potential to respond to the TGF-beta isoforms which have recently been detected in the endometrium in an autocrine and/or paracrine manner.
Dumont
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N
Department of Animal Science, McGill University, Montreal, Canada.
O'Connor-McCourt
M D
MD
Philip
A
A
D000345:Affinity Labels; D002455:Cell Division; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D004229:Dithiothreitol / Q000494:pharmacology; D004717:Endometrium / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D004848:Epithelium / Q000737:chemistry / Q000378:metabolism; D005260:Female; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D006801:Humans; D007163:Immunosorbent Techniques; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis* / Q000378:metabolism; D017154:Stromal Cells / Q000737:chemistry / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D000345:Affinity Labels; D003432:Cross-Linking Reagents; D017261:Glycosylphosphatidylinositols; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D010727:Phosphoric Diester Hydrolases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D004229:Dithiothreitol