title: Effect of Helicobacter mustelae infection on ferret gastric epithelial cell proliferation.
abstract: The effect of Helicobacter mustelae infection on gastric epithelial proliferation was studied in ferrets colonized with H.mustelae and specific pathogen-free (SPF) ferrets not infected with H.mustelae. Thirteen H. mustelae-infected ferrets between the ages of 13 and 32 months and 16 SPF ferrets between 6 and 18 months were analyzed. Bacterial cultures, urease tests and Warthin-Starry stains were used to identify H.mustelae. Tissues obtained from the antrum and the body regions of the stomach were assayed by proliferating cell nuclear antigen (PCNA) immunohistochemistry and measured using a computerized color image analysis system. PCNA-expressing gastric epithelia in the antrum and the body regions were significantly increased in the H.mustelae-infected ferrets versus the SPF ferrets (P < 0.001). PCNA positivity in the antrum regions of both the H.mustelae-infected ferrets and SPF ferrets was significantly higher than that of the body regions (P < 0.001). Comparison of the histopathology of infected ferrets indicated that PCNA positivity correlated with the histological severity of gastritis. This study suggests that cell proliferation in ferret gastric mucosa increases with H.mustelae infection and provides evidence that PCNA is a useful biomarker for studying the changes in cell kinetics in the ferret stomach. The data also further support the use of the H.mustelae-infected ferret as an animal model for studying the pathogenesis of Helicobacter pylori-induced gastric diseases of humans.
authors.affiliation: Gastrointestinal Nutrition Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA.
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.forename: R M
authors.initials: RM
authors.lastname: Russell
authors.forename: R N
authors.initials: RN
authors.lastname: Salomon
authors.forename: J C
authors.initials: JC
authors.lastname: Murphy
authors.forename: L S
authors.initials: LS
authors.lastname: Palley
authors.forename: J G
authors.initials: JG
authors.lastname: Fox
mesh_terms: D000818:Animals; D002455:Cell Division; D004848:Epithelium / Q000473:pathology; D005260:Female; D005289:Ferrets; D016481:Helicobacter Infections / Q000473:pathology*; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D013270:Stomach / Q000473:pathology*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D008769:Methylnitronitrosoguanidine