title: Autophosphorylation and activation of transcriptional activator PhoB of Escherichia coli by acetyl phosphate in vitro.
abstract: The PhoB protein, the transcriptional activator for the genes belonging to the phosphate regulon in Escherichia coli, was autophosphorylated in the presence of acetyl phosphate (acP) in vitro. The properties of phospho-PhoB, such as stability upon acid or alkali treatment and activating pstS transcription by RNA polymerase holoenzyme, were the same as those of phospho-PhoB produced from phospho-PhoR or phospho-PhoM. These results indicate that PhoB is an enzyme that catalyzes its own phosphorylation using acP, a low-molecular-weight metabolic intermediate.
authors.affiliation: Department of Molecular Microbiology, Osaka University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hiratsu
authors.forename: A
authors.initials: A
authors.lastname: Nakata
authors.forename: H
authors.initials: H
authors.lastname: Shinagawa
authors.forename: K
authors.initials: K
authors.lastname: Makino
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D010755:Organophosphates / Q000494:pharmacology*; D033902:Periplasmic Binding Proteins; D028044:Phosphate-Binding Proteins; D010766:Phosphorylation; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D010755:Organophosphates; D033902:Periplasmic Binding Proteins; D028044:Phosphate-Binding Proteins; C090133:PstS protein, E coli; D015534:Trans-Activators; C061278:PhoB protein, Bacteria; C011632:acetyl phosphate