Genomic organization of a mouse type I activin receptor.
We have characterized the genomic organization of a mouse type I activin receptor. Using the mouse tsk7L cDNA, 4 overlapping lambda clones containing the activin receptor IA (ActRIA) gene were isolated from a mouse 129 Sv genomic library. The mouse ActRIA gene is encoded by 10 exons and spans approximately 40 kb. The size of the introns was determined and the intron/exon boundaries were sequenced. Primer extension analysis of the 5' non-translated region using RNA from different organs or tissues revealed a strong transcription start site 68 nucleotides upstream of the ATG. Knowledge of the structure of the ActRIA gene is essential for the production of ActRIA deficient mice by homologous recombination.
Schmitt
J
J
Max-Planck-Institute for Immunobiology, Department of Developmental Biology, Freiburg, Germany.
Mielke
R
R
Schrewe
H
H
D029404:Activin Receptors; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000737:chemistry; D017931:DNA Primers; D005091:Exons; D015698:Genomic Library; D006801:Humans; D007438:Introns; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D009928:Organ Specificity; D014176:Protein Biosynthesis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D017978:Receptors, Growth Factor / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic
D017931:DNA Primers; D017978:Receptors, Growth Factor; D004247:DNA; D017346:Protein Serine-Threonine Kinases; D029404:Activin Receptors