Regulation of alternative splicing in the amyloid precursor protein (APP) mRNA during neuronal and glial differentiation of P19 embryonal carcinoma cells.
Cell-specific alteration in the splicing of exons 7, 8 and 15 of the amyloid precursor protein gene was investigated in differenting P19 EC cells into neuronal and glial cells. Exons 7 and 8 were skipped in the neuronal state and exon 15 was skipped in the glial state. Expression of U2AF, one of the essential factor for splicing in mammalian cells, was down-regulated during cellular differentiation. The skipping of exon 15 was suppressed in the glial cells transfected with U2AF. Thus, a reduction in U2AF is believed to play a crucial role in glial-specific splicing of the APP gene.
Tsukahara
T
T
Division of Neuromuscular Research, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Kunika
N
N
Momoi
T
T
Arahata
K
K
D017398:Alternative Splicing; D000682:Amyloid / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D018236:Carcinoma, Embryonal / Q000473:pathology*; D002454:Cell Differentiation / Q000502:physiology; D005091:Exons; D051379:Mice; D008969:Molecular Sequence Data; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D000072002:Prion Proteins; D011328:Prions; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured
D000682:Amyloid; D000072002:Prion Proteins; D011328:Prions; C512657:Prnp protein, mouse; D011498:Protein Precursors; D012333:RNA, Messenger