title: Interleukin-8 increases endothelial permeability independent of neutrophils.
abstract: Interleukin-8 (IL-8) has been associated with a variety of hyperinflammatory states and adverse clinical events. Circulating IL-8 levels correlate with the severity of tissue trauma, and excessive elevations of IL-8 are associated with postinjury adult respiratory distress syndrome and multiple organ failure. While IL-8 is a potent neutrophil (PMN) chemoattractant and activator and enhances PMN transendothelial migration, it also acts to inhibit PMN adhesion to stimulated endothelial cells (ECs). We hypothesized that IL-8 could interact directly with ECs to increase permeability independent of PMNs. Human umbilical vein ECs (HUVECs) were cultured on collagen-coated micropore filters, and integrity of the EC monolayer measured by albumin flux across the filter. Cytochalasin D was used as a positive control. IL-8 induced increased permeability at a concentration of 1000 ng/mL. This effect was abrogated by preincubation of HUVECs with a protein synthesis inhibitor (cycloheximide). These data suggest a role for IL-8 in promoting endothelial leak independent of PMNs, via a mechanism involving protein synthesis.
authors.affiliation: Department of Surgery, Denver General Hospital, CO 80204-4507, USA.
authors.forename: W L
authors.initials: WL
authors.lastname: Biffl
authors.forename: E E
authors.initials: EE
authors.lastname: Moore
authors.forename: F A
authors.initials: FA
authors.lastname: Moore
authors.forename: V S
authors.initials: VS
authors.lastname: Carl
authors.forename: R J
authors.initials: RJ
authors.lastname: Franciose
authors.forename: A
authors.initials: A
authors.lastname: Banerjee
mesh_terms: D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D015638:Cytochalasin D / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects*; D014471:Umbilical Veins / Q000187:drug effects
chemical_list: D016209:Interleukin-8; D015638:Cytochalasin D