Emergence of nylon oligomer degradation enzymes in Pseudomonas aeruginosa PAO through experimental evolution.
Through selective cultivation with 6-aminohexanoate linear dimer, a by-product of nylon-6 manufacture, as the sole source of carbon and nitrogen, Pseudomonas aeruginosa PAO, which initially has no enzyme activity to degrade this xenobiotic compound, was successfully expanded in its metabolic ability. Two new enzyme activities, 6-aminohexanoate cyclic dimer hydrolase and 6-aminohexanoate dimer hydrolase, were detected in the adapted strains.
Prijambada
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Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.
Negoro
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Yomo
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Urabe
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D000581:Amidohydrolases / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001673:Biodegradation, Environmental; D005075:Biological Evolution; D004734:Energy Metabolism; D007218:Industrial Microbiology; D009757:Nylons / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000235:genetics; D012641:Selection, Genetic; D013379:Substrate Specificity; D015262:Xenobiotics / Q000378:metabolism
D001426:Bacterial Proteins; D009757:Nylons; D015262:Xenobiotics; D000581:Amidohydrolases; C026250:6-aminohexanoate-dimer hydrolase; C019683:6-aminohexanoate-cyclic-dimer hydrolase