Effect of glibenclamide on the metabolism of fatty acids in cultures of newborn rat heart cells under normoxic and hypoxic conditions.
Several deleterious biochemical alterations have been observed in myocardial cells during ischemia, including perturbations of transmembrane ion equilibria, production of noxious oxygen-derived radicals and loss of membrane phospholipids. Although the precise relationship between these alterations and the reduction of oxygen and glucose supplies is not fully understood, the decrease of intracellular ATP content appears to be a key event in the cascade. Recent evidence suggests that opening of ATP-sensitive K+ channels may constitute an endogenous protective mechanism during ischemia. We have thus tested the effects of glibenclamide, a channel blocker, and aprikalim, a channel opener, on the metabolism of membrane fatty acids in cultures of newborn rat heart cells under normoxic and hypoxic conditions. We showed that glibenclamide partially blocks the loss of membrane phospholipids induced by oxygen deprivation in contractile myocytes, whereas aprikalim fails to alter this metabolism under either normoxic or ischemic conditions. In cultures of fibroblast-like heart cells neither drug was able to modify fatty acid metabolism.
Freyss-Beguin
M
M
CNRS URA 1482, Faculté de Médecine Necker-Enfants Malades, Paris, France.
Simon
J
J
Duval
D
D
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D016718:Arachidonic Acid / Q000378:metabolism; D015687:Cell Hypoxia; D002478:Cells, Cultured; D005227:Fatty Acids / Q000378:metabolism*; D005905:Glyburide / Q000494:pharmacology*; D050356:Lipid Metabolism; D009200:Myocardial Contraction; D009206:Myocardium / Q000378:metabolism*; D010100:Oxygen / Q000008:administration & dosage; D010847:Picolines / Q000494:pharmacology; D011714:Pyrans / Q000494:pharmacology; D051381:Rats
D005227:Fatty Acids; D010847:Picolines; D011714:Pyrans; D016718:Arachidonic Acid; C061043:aprikalim; D000255:Adenosine Triphosphate; D010100:Oxygen; D005905:Glyburide