title: Identification of Entamoeba histolytica intracellular phospholipase A and lysophospholipase L1 activities.
abstract: Entamoeba histolytica phospholipase A and lysophospholipase activities from a vesicular subcellular fraction (P30) were analyzed. The products, obtained using specific substrates labeled with 14C or 3H, indicated the presence of phospholipase A1 and A2 as well as lysophospholipase L1 activities. The enzymes detected could participate in phospholipid metabolism and the alkaline phospholipase A2 may contribute to E. histolytica cytopathogenicity.
authors.affiliation: División de Biología Celular y Molecular, Instituto Mexicano del Seguro Social, Monterrey, N.L. México.
authors.forename: J
authors.initials: J
authors.lastname: Vargas-Villarreal
authors.forename: H
authors.initials: H
authors.lastname: Martínez-Rodríguez
authors.forename: J
authors.initials: J
authors.lastname: Castro-Garza
authors.forename: B D
authors.initials: BD
authors.lastname: Mata-Cárdenas
authors.forename: M T
authors.initials: MT
authors.lastname: González-Garza
authors.forename: S
authors.initials: S
authors.lastname: Said-Fernández
mesh_terms: D000818:Animals; D002122:Calcium Chloride / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000648:ultrastructure; D006868:Hydrolysis; D008245:Lysophospholipase / Q000378:metabolism*; D010741:Phospholipases A / Q000187:drug effects / Q000378:metabolism*; D054466:Phospholipases A1; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D010743:Phospholipids; D010741:Phospholipases A; D054466:Phospholipases A1; D054467:Phospholipases A2; D008245:Lysophospholipase; D002122:Calcium Chloride