title: Multiple genes code for high-molecular-mass rhoptry proteins of Plasmodium yoelii.
abstract: We have examined the number of genes coding for a group of high-molecular-mass rhoptry protein(s) in the malaria parasite Plasmodium yoelii, and studied variation in the gene family within the parasite's genome. A region of the genes was amplified using oligonucleotides based on conserved DNA sequences and the products cloned. The sequences could be divided into 7 groups by restriction-fragment-length polymorphism. Further variation was detected by sequence analysis; 11 different sequences were detected in the 16 clones analyzed. The genes in the family were distributed on 6 chromosomes probably at 9 or more loci.
authors.affiliation: Division of Parasitology, National Institute for Medical Research, London, UK.
authors.forename: M B
authors.initials: MB
authors.lastname: Borre
authors.forename: C A
authors.initials: CA
authors.lastname: Owen
authors.forename: J K
authors.initials: JK
authors.lastname: Keen
authors.forename: K A
authors.initials: KA
authors.lastname: Sinha
authors.forename: A A
authors.initials: AA
authors.lastname: Holder
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017125:Genes, Protozoan; D014644:Genetic Variation; D051379:Mice; D008969:Molecular Sequence Data; D010967:Plasmodium yoelii / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics* / Q000276:immunology; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D015800:Protozoan Proteins