LRP1, a gene expressed in lateral and adventitious root primordia of arabidopsis.
We describe a gene that is expressed in lateral and adventitious root primordia of Arabidopsis. The gene was identified by expression of a transposon-borne promoterless beta-glucuronidase gene in lateral root primordia. The gene, designated LRP1 for lateral root primordium 1, and its corresponding cDNA were cloned and sequenced. The expression pattern of the gene in lateral root primordia was confirmed by in situ hybridization with LRP1 cDNA probes. The LRP1 gene encodes a novel protein. LRP1 expression is activated during the early stages of root primordium development and is turned off prior to the emergence of lateral roots from the parent root. Insertion of the transposon in the LRP1 gene disrupted its expression. To evaluate the homozygous insertion line for a mutant phenotype, several aspects of wild-type lateral root development were analyzed. A mutant phenotype has not yet been identified in the insertion line; however, there is evidence that the gene belongs to a small gene family. LRP1 provides a molecular marker to study the early stages of lateral and adventitious root primordium development.
Smith
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DL
Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA.
Fedoroff
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NV
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D018744:DNA, Plant; D004742:Enhancer Elements, Genetic; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D006720:Homozygote; D008969:Molecular Sequence Data; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*; D018517:Plant Roots / Q000378:metabolism*
D029681:Arabidopsis Proteins; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D018744:DNA, Plant; C095307:LRP1 protein, Arabidopsis; D010940:Plant Proteins; D005966:Glucuronidase