A novel chemokine, macrophage inflammatory protein-related protein-2, inhibits colony formation of bone marrow myeloid progenitors.
A new member of the beta-chemokine family, macrophage inflammatory protein (MIP)-related protein-2 (MRP-2) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 is composed of 122 amino acids of which the first 21 residues constitute a putative signal sequence. The putative mature protein is composed of 101 amino acids with a molecular weight of 11,600. MRP-2 is structurally similar to MIP-related protein-1 (MRP-1) (C10) and MIP-1 alpha. MRP-2 shows a 50.8% sequence identity at the protein level to MRP-1 and 46.3% identity to MIP-1 alpha. MRP-2 detects approximately 1.3 kilobase mRNA from monocyte and macrophage cell lines but does not detect the mRNA from T and B cells. The MRP-2 gene termed Scya9 was mapped to the central region of mouse chromosome 11 near the Scya1 and Scya2 genes, which are also members of the beta-chemokine superfamily. The Scya gene cluster was located between neurofibromatosis type 1 (Nf1) and myeloperoxidase (Mpo). rMRP-2 significantly suppressed colony formation by murine and human bone marrow granulocyte-macrophage (CFU-granulocyte-macrophage), erythroid (burst-forming unit-E), and multipotential (CFU-granulocyte-erythroid-macrophage-megakaryocyte) progenitor cells stimulated by combinations of growth factors.
Youn
B S
BS
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202, USA.
Jang
I K
IK
Broxmeyer
H E
HE
Cooper
S
S
Jenkins
N A
NA
Gilbert
D J
DJ
Copeland
N G
NG
Elick
T A
TA
Fraser
M J
MJ
Kwon
B S
BS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D054407:Chemokine CCL4; D019742:Chemokines, CC; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003114:Colony-Forming Units Assay; D016207:Cytokines / Q000819:agonists* / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D019402:Macrophage Inflammatory Proteins; D051379:Mice; D008969:Molecular Sequence Data; D015846:Monokines / Q000819:agonists* / Q000494:pharmacology*; D000089762:Multidrug Resistance-Associated Protein 2
C000715087:ABCC2 protein, human; C095218:Ccl9 protein, mouse; D054407:Chemokine CCL4; D019742:Chemokines, CC; D016207:Cytokines; D019402:Macrophage Inflammatory Proteins; D015846:Monokines; D000089762:Multidrug Resistance-Associated Protein 2