Novel mutation in the UL97 gene of a clinical cytomegalovirus strain conferring resistance to ganciclovir.
Characterization of a ganciclovir-resistant cytomegalovirus strain from a patient with AIDS showed a histidine-to-glutamine change at residue 520 of UL97 (Q520 mutation). In anabolism studies, Q520 was associated with impaired phosphorylation of ganciclovir. Transfer of Q520 to a recombinant virus resulted in a ganciclovir-resistant phenotype.
Hanson
M N
MN
Department of Laboratory Medicine, University of Minnesota Medical School, Minneapolis 55455-0392, USA.
Preheim
L C
LC
Chou
S
S
Talarico
C L
CL
Biron
K K
KK
Erice
A
A
D017088:AIDS-Related Opportunistic Infections / Q000382:microbiology; D001483:Base Sequence; D003587:Cytomegalovirus / Q000187:drug effects* / Q000235:genetics*; D004352:Drug Resistance, Microbial; D015774:Ganciclovir / Q000494:pharmacology*; D005973:Glutamine / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D016133:Polymerase Chain Reaction
D005973:Glutamine; D006639:Histidine; D015774:Ganciclovir