[Microbiological assay method for T-3761 concentration in body fluids].
A microbiological assay method for measurement of T-3761 and its stability in body fluids were investigated. The paper disc method proved suitable for this assay using Escherichia coli Kp as a test organism and commercially available heart infusion agar as a test medium. When using the paper disc method, lower detection limit of T-3761 was approximately 0.05 microgram/ml and 0.1 microgram/ml for 1/15M phosphate buffer (pH 7.0) and human serum, respectively. T-3761 in human serum and urine was stable under freezing -20 degrees C for at least 28 days.
Maehana
J
J
Research Laboratories, Toyama Chemical Co., Ltd.
Kitayama
R
R
Matsumoto
Y
Y
Kumano
K
K
Minami
S
S
Yasuda
T
T
Watanabe
Y
Y
Narita
H
H
D000362:Agar; D000890:Anti-Infective Agents; D001681:Biological Assay / Q000379:methods; D001826:Body Fluids / Q000378:metabolism*; D003470:Culture Media; D004352:Drug Resistance, Microbial; D004355:Drug Stability; D004926:Escherichia coli / Q000187:drug effects; D024841:Fluoroquinolones; D006801:Humans; D010078:Oxazines / Q000493:pharmacokinetics* / Q000494:pharmacology
D000890:Anti-Infective Agents; D003470:Culture Media; D024841:Fluoroquinolones; D010078:Oxazines; C078052:pazufloxacin; D000362:Agar