A 67-kDa protein binding to the curved DNA in the main regulatory region of the rat mitochondrial genome.
We have purified, by sequence-specific affinity chromatography, a mitochondrial (mt) matrix protein which binds to the curved DNA located between the replication origin (ori) of the leading strand (ori-H) and the two transcription promoters in the rat mt genome. The protein was characterized by gel electrophoresis as a 67-kDa polypeptide and seems to be involved in the DNA contact on the mt light strand. This protein differs (in the size and location of its DNA-binding site) from other DNA-binding proteins studied so far in animal mt systems. We suggest a role for the 67-kDa protein, assisted by other proteins, in regulating the initiation of leading-strand replication.
Gadaleta
G
G
CSMME-CNR, Universitá di Bari, Italy.
D'Elia
D
D
Saccone
C
C
Pepe
G
G
D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology; D008930:Mitochondria, Liver / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D051381:Rats / Q000235:genetics / Q000378:metabolism*; D017208:Rats, Wistar; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000302:isolation & purification* / Q000378:metabolism / Q000502:physiology
D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins; D014157:Transcription Factors; C095201:mitochondrial matrix protein, 67-kDa, rat