Regulation of the glnBA operon of Rhodobacter capsulatus.
In a recent report identifying the promoters of the Rhodobacter capsulatus glnBA operon, it was suggested that an internal promoter upstream of the glnA gene probably resulted in different levels of glnBA and glnA transcripts (D. Foster-Hartnett and R. G. Kranz, J. Bacteriol. 176:5171-5176, 1994). Therefore, to investigate the regulation, we constructed and examined the expression of a number of translational fusions in R. capsulatus glnBA. The results support a role for posttranscriptional regulation.
Borghese
R
R
Biochemistry Department, University of Missouri-Columbia 65211, USA.
Wall
J D
JD
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005809:Genes, Regulator; D005974:Glutamate-Ammonia Ligase / Q000096:biosynthesis / Q000235:genetics; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009876:Operon; D051922:PII Nitrogen Regulatory Proteins; D012333:RNA, Messenger / Q000235:genetics; D016354:Rhodobacter capsulatus / Q000235:genetics*
D001426:Bacterial Proteins; D051922:PII Nitrogen Regulatory Proteins; D012333:RNA, Messenger; C404143:PIID regulatory protein, Bacteria; C081173:glutamine synthetase I; D005974:Glutamate-Ammonia Ligase