title: Accumulation of gamma/delta T cells in human dysgerminoma and seminoma: roles in autologous tumor killing and granuloma formation.
abstract: The precise biological function of a subset of T cells bearing gamma/delta T cell receptor (TCR) remains poorly understood. The present study demonstrated the presence of gamma/delta T cells in tumor-infiltrating lymphocytes (TIL) and peripheral blood lymphocytes (PBL) of human patients with dysgerminoma and seminoma when determined by flow cytometry and in situ immunohistochemical staining. TIL contained a high percentage of gamma/delta T cells, ranging from 17.3 to 35.1%. gamma/delta T cells often accumulated within the granulomatous inflammation of tumor tissues. The majority of gamma/delta T cells were V gamma 9/V delta 2+ cells. Freshly isolated PBL, TIL and purified gamma/delta T cells showed autologous tumor killing (ATK) activity, which could be inhibited by monoclonal antibodies (mAb) against V delta 2. Furthermore, two gamma/delta T cell clones established from TIL showed cytotoxicity against autologous and allogeneic dysgerminoma, while they had low or no lytic effects on other cell types including carcinomas of ovary and tumor cell lines such as K562, Daudi and Molt-4. Lysis of autologous tumor cells by the clone was inhibited completely by anti-V delta 2 mAb and partially by mAb against CD3, LFA-1 alpha and ICAM-1 molecules, while it was resistant to anti-CD8, anti-HLA-ABC and anti-HLA-DR mAb. Supernatants produced by gamma/delta T cell clones induced adhesion, aggregation and increased DNA synthesis of monocytes and some characteristics of activated macrophages or epithelioid cells. Tumor necrosis factor (TNF)-alpha, granulocyte-macrophage colony stimulating factor (GM-CSF) and interferon (IFN)-gamma were detected in the supernatants of gamma/delta T cell clone. These results suggest that gamma/delta T cells accumulating in dysgerminoma and seminoma exhibit ATK activity through V gamma 9/delta 2 TCR and these gamma/delta T cells also play a role in the formation of granulomatous inflammation, which is associated with human dysgerminoma and seminoma.
authors.affiliation: Department of Late Effect Studies, Kyoto University, Japan.
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: Y Q
authors.initials: YQ
authors.lastname: Wei
authors.forename: Y
authors.initials: Y
authors.lastname: Kariya
authors.forename: K
authors.initials: K
authors.lastname: Teshigawara
authors.forename: A
authors.initials: A
authors.lastname: Uchida
mesh_terms: D000328:Adult; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001932:Brain Neoplasms / Q000276:immunology* / Q000473:pathology; D002999:Clone Cells / Q000276:immunology / Q000473:pathology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016207:Cytokines / Q000302:isolation & purification / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004407:Dysgerminoma / Q000097:blood / Q000276:immunology* / Q000473:pathology; D005260:Female; D006099:Granuloma / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008213:Lymphocyte Activation; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000276:immunology* / Q000473:pathology; D010870:Pineal Gland / Q000473:pathology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis*; D018239:Seminoma / Q000097:blood / Q000276:immunology* / Q000473:pathology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000473:pathology; D013736:Testicular Neoplasms / Q000276:immunology* / Q000473:pathology; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned; D016207:Cytokines; D016692:Receptors, Antigen, T-Cell, gamma-delta