title: Effect of luteinizing hormone and estradiol on in vitro maturation of follicular oocytes in goat (Capra hircus).
abstract: In vitro maturation of goat follicular oocytes in TALP medium supplemented with variable concentrations of luteinizing hormone (LH) [5-20 micrograms/ml] and estradiol [1 microgram/ml] was studied. Incubation of viable oocytes was carried out at 38.5 degrees C under liquid paraffin in 5% carbon dioxide/air mixture for 24 h. At 0 h all the oocytes were in germinal vesicle (GV) stage, each with a good cumulus. The cumulus expansion increased linearly with increasing concentrations of LH and a fixed concentration of estradiol [1 microgram/ml] at 24 h of incubation. Germinal vesicle breakdown (GVBD) was observed in 28% of the oocytes at 5 micrograms/ml concentration of LH and the same increased to 87% at 10 micrograms/ml of LH with 13% oocytes at metaphase II. At 20 micrograms/ml of LH, 45% of the oocytes were at metaphase II and the rest were at metaphase I. LH and estradiol seem to be important for acquisition of maturation competence in goat follicular oocytes.
authors.affiliation: Department of Zoology, Punjab Agricultural University, Ludhiana, India.
authors.forename: K S
authors.initials: KS
authors.lastname: Sidhu
authors.forename: S
authors.initials: S
authors.lastname: Cheema
mesh_terms: D000818:Animals; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D006041:Goats / Q000502:physiology*; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000494:pharmacology*; D008677:Metaphase / Q000187:drug effects; D009865:Oocytes / Q000166:cytology / Q000187:drug effects*; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects*
chemical_list: D004958:Estradiol; D007986:Luteinizing Hormone