Cloning and characterization of a human type II receptor for bone morphogenetic proteins.
Bone morphogenetic proteins (BMPs) are members of the transforming growth factor beta superfamily. Several members of this family have been shown to transduce their signals through binding to type I and type II serine-(threonine) kinase receptors. Here we report the cDNA cloning and characterization of a human type II receptor for BMPs (BMPR-II), which is distantly related to DAF-4, a BMP type II receptor from Caenorhabditis elegans. In transfected COS-1 cells, osteogenic protein (OP)-1/BMP-7, and less efficiently BMP-4, bound to BMPR-II. BMPR-II bound ligands only weakly alone, but the binding was facilitated by the presence of previously identified type I receptors for BMPs. Binding of OP-1/BMP-7 to BMPR-II was also observed in nontransfected cell lines. Moreover, a transcriptional activation signal was transduced by BMPR-II in the presence of type I receptors after stimulation by OP-1/BMP-7.
Rosenzweig
B L
BL
Synergen, Inc., Boulder, CO 80301-2546, USA.
Imamura
T
T
Okadome
T
T
Cox
G N
GN
Yamashita
H
H
ten Dijke
P
P
Heldin
C H
CH
Miyazono
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D052004:Bone Morphogenetic Protein Receptors; D019485:Bone Morphogenetic Proteins; D002460:Cell Line; D003001:Cloning, Molecular / Q000379:methods; D017931:DNA Primers; D018076:DNA, Complementary; D005909:Glioblastoma; D006133:Growth Substances / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D017978:Receptors, Growth Factor; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013378:Substantia Nigra / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection
D019485:Bone Morphogenetic Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D006133:Growth Substances; D011506:Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D017978:Receptors, Growth Factor; D011994:Recombinant Proteins; D052004:Bone Morphogenetic Protein Receptors