The 5'-untranslated region sequence of a potential new genotype of bovine viral diarrhea virus.
The 5' untranslated region (UTR) of several bovine viral diarrhea virus (BVDV) isolates from the severe Quebec outbreak was amplified by polymerase chain reaction (PCR) and sequenced. Sequences revealed the loss, for the BVDV type II isolates, of an internal PstI restriction site, which is present in all known BVDV type 1 5' UTR sequences. A single restriction enzyme digestion (PstI) of an aliquot of PCR product allowed us to differentiate BVDV type I and BVDV type II.
Harpin
S
S
Department of Pathology and Immunology, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, Quebec, Canada.
Elahi
S M
SM
Cornaglia
E
E
Yolken
R H
RH
Elazhary
Y
Y
D000818:Animals; D001483:Base Sequence; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology / Q000821:virology; D002417:Cattle; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004196:Disease Outbreaks / Q000662:veterinary; D005838:Genotype; D007422:Intestines / Q000821:virology; D008168:Lung / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011792:Quebec / Q000453:epidemiology; D015183:Restriction Mapping; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen / Q000821:virology