Phase-sensitive two-dimensional experiment on living animals.
This report describes the first 1H-1H phased spectrum obtained in vivo from the hind-leg muscles of an intact mouse. TOCSY correlations can follow the complete spin system and give a more detailed graph of each molecule than can COSY. TOCSY is also better suited than COSY for studying long fatty-acid chains. All the peaks are in phase in a TOCSY experiment and the intensities of the cross-correlation peaks are less sensitive to low digitalization in the t1 domain than are those of COSY. The improvement in sensitivity was estimated by measuring the volumes of the cross-correlation peaks in COSY and TOCSY spectra.
Gillet
B
B
Laboratoire de RMN biologique, ICSN-CNRS, Gif Sur Yvette, France.
Doan
B T
BT
D000818:Animals; D002336:Carnosine / Q000378:metabolism; D003903:Deuterium; D005227:Fatty Acids / Q000378:metabolism; D006614:Hindlimb / Q000378:metabolism; D006859:Hydrogen; D007089:Image Enhancement / Q000379:methods*; D007773:Lactates / Q000378:metabolism; D019787:Linoleic Acid; D008041:Linoleic Acids / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D013654:Taurine / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism
D005227:Fatty Acids; D007773:Lactates; D008041:Linoleic Acids; D010713:Phosphatidylcholines; D014280:Triglycerides; D013654:Taurine; D006859:Hydrogen; D002336:Carnosine; D019787:Linoleic Acid; D003903:Deuterium