title: Dietary manipulation of long-chain polyunsaturated fatty acids in the retina and brain of guinea pigs.
abstract: High levels of n-6 docosapentaenoic acid (22:5n-6) have been reported in the retina of guinea pigs fed commercially-prepared gain-based rations (commercial diet). In rats and monkeys, high levels of 22:5n-6 are an indicator of n-3 polyunsaturated fatty acid (PUFA) deficiency. We have examined the fatty acid composition of the retina and brain in guinea pigs fed a commercial diet or one of three semi-purified diets containing three different levels of n-3 PUFA. The diets comprised a diet deficient in n-3 PUFA (semi-purified diet containing safflower oil), two diets containing alpha-linolenic acid (standard commercial laboratory diet and semi-purified diet containing canola oil), and a diet containing alpha-linolenic acid, eicosapentaenoic acid, and docosahexaenoic acid (DHA) (semi-purified diet containing canola oil, safflower oil, and fish oil). Two groups of guinea pigs were given the diets from day 1 to 4 wk or day 1 to 8 wk, when they were sacrificed and the retinal tissues were extracted and analyzed for PUFA content by gas-liquid chromatography. Fatty acid analyses of the retinal phospholipids of the four-week-old animals revealed that the group fed DHA (from the fish oil) had the highest level of DHA (32%), compared with values of 19 and 13% for the groups fed canola oil diet and commercial diet, respectively, and 2% for the group fed the diet deficient in n-3 PUFA.(ABSTRACT TRUNCATED AT 250 WORDS)
authors.affiliation: Department of Optometry, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: H S
authors.initials: HS
authors.lastname: Weisinger
authors.forename: A J
authors.initials: AJ
authors.lastname: Vingrys
authors.forename: A J
authors.initials: AJ
authors.lastname: Sinclair
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D004281:Docosahexaenoic Acids / Q000008:administration & dosage; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage; D005231:Fatty Acids, Unsaturated / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D010743:Phospholipids / Q000737:chemistry; D012160:Retina / Q000737:chemistry / Q000378:metabolism*; D017962:alpha-Linolenic Acid / Q000008:administration & dosage
chemical_list: D004042:Dietary Fats, Unsaturated; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D010743:Phospholipids; D017962:alpha-Linolenic Acid; D004281:Docosahexaenoic Acids