Molecular mechanisms of sepsis: molecular biology of the cell.
Complex and interrelated biological processes are at work in the expression of the host response to sepsis. To a large degree, these processes reflect drastic changes in the molecular workings of cells of the body. The protean nature of sepsis reflects this molecular adaptation. Studies are continuing to accrue that describe aspects of this process in tissue culture, animal models, and man. However, without an understanding of the basic mechanisms of molecular biology, the understanding of this important and expanding literature is limited. This review describes the basic molecular processes involved in replication of deoxyribonucleic acid (DNA) and transcription of DNA to ribonucleic acid (RNA) in the nucleus, translation of messenger RNA into proteins and the posttranslational modifications of these proteins in the cytoplasm. It uses the process of endotoxin-induced cellular activation as its model and highlights important aspects of DNA promoter and enhancer processes in this activation. Specific examples of known promoter genes and genomic translation are described. This review serves as a "primer" for the subsequent three review articles in this series that will follow it in preceding issues.
Hayashi
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S
Pulmonary Research Laboratory, St Paul's Hospital, British Columbia, Canada.
Gillam
I C
IC
Bondy
G
G
Duronio
V
V
Hogg
J C
JC
D000818:Animals; D001483:Base Sequence; D004261:DNA Replication; D004195:Disease Models, Animal; D006801:Humans; D008967:Molecular Biology; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D018805:Sepsis / Q000503:physiopathology*; D015398:Signal Transduction; D014158:Transcription, Genetic