Separation of basic drugs by packed-column supercritical fluid chromatography. 3. Stimulants.
A wide range of stimulants was rapidly and efficiently separated with symmetrical peak shapes with a tertiary supercritical mobile phase and a cyanopropyl liquid chromatography column. Compared with other drug families studied in this series, the stimulants exhibited a much broader range of retention. Several strong bases, including primary aliphatic amines, were easily eluted with a modified mobile phase. The stimulants were generally more retained than antipsychotic drugs. Compared with antidepressant drugs, a few stimulants behaved similarly, but most were more strongly retained. Modifier concentration proved to be the most effective means for changing both retention and selectivity. An over-the-counter eye drop solution (an aqueous buffer) was analyzed for tetrahydrozoline. The positive results with good peak shapes indicate that packed-column supercritical fluid chromatography is compatible with at least small aqueous samples.
Berger
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Hewlett Packard Company, Wilmington, DE 19808-1610, USA.
Wilson
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D000697:Central Nervous System Stimulants / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid; D007202:Indicators and Reagents; D011312:Pressure; D012996:Solutions; D013696:Temperature
D000697:Central Nervous System Stimulants; D007202:Indicators and Reagents; D012996:Solutions