Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California.
136 fecal samples, collected from 47 dairy calves on a calf ranch and in a dairy herd in California, were tested for the presence of group A and group B rotaviruses by reverse transcription-polymerase chain reaction (RT-PCR). Samples were collected from each calf at days 1, 7 and 14. Within the 14 day period, 44 calves (94%) were positive for group A rotavirus and an unexpectedly high number of calves (38 calves, 81%) were positive for group B rotavirus. When these samples were examined by polyacrylamide gel electrophoresis (PAGE), rotavirus was found in 21 calves and all of them had group A electropherotype. Among 25 PAGE positive samples from 21 calves, 17 (68%) were of short electropherotype, 4 (28%) were of long electropherotype and 4 (28%) contained both short and long electropherotype rotaviruses. Group B and short and long electropherotype group A rotaviruses were found in both normal and diarrheic calves.
Chinsangaram
J
J
Department of Veterinary Pathology, School of Veterinary Medicine, University of California, Davis, USA.
Schore
C E
CE
Guterbock
W
W
Weaver
L D
LD
Osburn
B I
BI
D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D002140:California / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000821:virology; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D003967:Diarrhea / Q000453:epidemiology / Q000662:veterinary / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D005243:Feces / Q000382:microbiology*; D005260:Female; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D014158:Transcription, Genetic
D017931:DNA Primers; D004279:DNA, Viral