Detection of FMRFamide-like immunoreactivities in the sea scallop Placopecten magellanicus by immunohistochemistry and western blot analysis.
FMRFamide-like immunoreactivity was detected histochemically in the sea scallop Placopecten magellanicus. Most immunoreactivity was concentrated in the cerebral, pedal, and parietovisceral ganglia, particularly in the cortical cell bodies and in their fibers which extend into the central neuropile. Whole-mount immunofluorescence studies were used to localize concentrations of immunoreactive cells on the dorsal and ventral surfaces of each ganglion. Immunoreactivity was also detected in nerves emanating from the ganglia. Strong immunoreactivity was localized in peripheral organs, including the gut and gills of juvenile and adult scallops. Weak immunoreactivity was detected in the gonads, heart, and adductor muscle of the adults. A broad FMRFamide-like immunoreactive band of 2.5-8.2 kDa was detected by Western blotting of acetone extracts of the parietovisceral ganglia. In the presence of protease inhibitors, two FMRFamide-like immunoreactive bands (7.2-8.2 kDa and > 17 kDa) were obtained. Neither of these bands comigrated with the FMRFamide standard. It is concluded that peptides of the FMRFamide family are probably regulators of numerous central and peripheral functions in P. magellanicus.
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Department of Physiology and Biophysics, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada.
Croll
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RP
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D002490:Central Nervous System / Q000737:chemistry / Q000166:cytology; D019835:FMRFamide; D005260:Female; D007124:Immunoenzyme Techniques; D007447:Invertebrate Hormones / Q000032:analysis* / Q000276:immunology; D008297:Male; D008974:Mollusca / Q000737:chemistry*; D009479:Neuropeptides / Q000032:analysis* / Q000276:immunology; D010053:Ovary / Q000737:chemistry / Q000166:cytology; D013737:Testis / Q000737:chemistry / Q000166:cytology
D000906:Antibodies; D007447:Invertebrate Hormones; D009479:Neuropeptides; D019835:FMRFamide