Identification of a plant serine-arginine-rich protein similar to the mammalian splicing factor SF2/ASF.
We show that the higher plant Arabidopsis thaliana has a serine-arginine-rich (SR) protein family whose members contain a phosphoepitope shared by the animal SR family of splicing factors. In addition, we report the cloning and characterization of a cDNA encoding a higher-plant SR protein from Arabidopsis, SR1, which has striking sequence and structural homology to the human splicing factor SF2/ASF. Similar to SF2/ASF, the plant SR1 protein promotes splice site switching in mammalian nuclear extracts. A novel feature of the Arabidopsis SR protein is a C-terminal domain containing a high concentration of proline, serine, and lysine residues (PSK domain), a composition reminiscent of histones. This domain includes a putative phosphorylation site for the mitotic kinase cyclin/p34cdc2.
Lazar
G
G
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA.
Schaal
T
T
Maniatis
T
T
Goodman
H M
HM
D000595:Amino Acid Sequence; D000818:Animals; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins; D001120:Arginine; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005091:Exons; D006801:Humans; D008239:Lysine; D008322:Mammals; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011392:Proline; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D012694:Serine; D000068103:Serine-Arginine Splicing Factors; D017381:Spliceosomes / Q000378:metabolism; D014157:Transcription Factors
D029681:Arabidopsis Proteins; D001120:Arginine; D018076:DNA, Complementary; D008239:Lysine; D009687:Nuclear Proteins; D010940:Plant Proteins; D011392:Proline; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins; D012694:Serine; D000068103:Serine-Arginine Splicing Factors; C572689:AT2G22300 protein, Arabidopsis; D014157:Transcription Factors