Determination of serum retinol by reversed-phase high-performance liquid chromatography.
A rapid, sensitive and specific high-performance liquid chromatographic method was developed for the determination of serum levels of retinol in humans. A direct serum injection technique after deproteinisation was used to avoid lengthy pretreatment steps which can result in degradation of retinol during analysis. The column used was CLC-ODS, the mobile phase was acetonitrile-water and detection wavelength was 328 nm. Deterioration in column performance was not observed even after injection of 300 samples. The lower detection limit was 10 micrograms/l. On analyzing a serum pool six times, a C.V. of 0.7% was obtained. The method is quantitative, reproducible, rapid and highly accurate for routine analysis.
Siddiqui
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FQ
Drugs Control and Traditional Medicines Division, National Institute of Health, Islamabad, Pakistan.
Malik
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F
Fazli
F R
FR
D002675:Child, Preschool; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D015203:Reproducibility of Results; D014801:Vitamin A / Q000097:blood*
D014801:Vitamin A