Light and development regulate the expression of the albino-3 gene in Neurospora crassa.
The albino-3 gene of Neurospora crassa codes for geranylgeranyl pyrophosphate synthase, an enzyme involved in the biosynthesis of carotenoids. The albino-3 locus encodes two overlapping transcription units that give rise to two mRNAs of 2.2 kb (al-3(c)) and 1>6 kb (al-3(m)), with the promoter of the latter residing in the transcribed region of the former. The 1.6-kb transcript was transiently expressed in the mycelium after light induction, while the 2.2-kb mRNA appeared in conidiating cultures only, independently of light. Al-3(c) mRNA was enhanced in the conidiophores and was impaired in mutants blocked at the early stages of conidiation; al-3(c) therefore represents a conidiation-specific transcript. The al-3(c) mRNA level increased upon illumination with blue light and fluctuated according to the circadian cycle.
Arpaia
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Dipartimento di Biopatologia Umana, Policlinico Umberto I, Università di Roma La Sapienza, Italy.
Carattoli
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Macino
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D001483:Base Sequence; D002874:Chromosome Mapping; D002940:Circadian Rhythm / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004122:Dimethylallyltranstransferase / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000528:radiation effects; D015966:Gene Expression Regulation, Fungal / Q000528:radiation effects; D005800:Genes, Fungal / Q000528:radiation effects; D008027:Light; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development / Q000528:radiation effects; D016133:Polymerase Chain Reaction; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D017931:DNA Primers; D004271:DNA, Fungal; D012331:RNA, Fungal; D012333:RNA, Messenger; D004122:Dimethylallyltranstransferase