[Demonstration of cells of the mononuclear phagocyte system of the cat using enzyme and immunohistochemistry methods].
The suitability of enzyme- and immunohistochemical methods for the demonstration of mononuclear phagocytes in cats is treated. The activity of nonspecific esterases with the substrates alpha-naphthyl-acetate, alpha-naphthyl-butyrate and naphthol-AS-acetate, and of acid phosphatases with the substrate naphthol-AS-BI-phosphate is demonstrated in paraffin and plastic sections of the liver, lungs, spleen and lymph node. Even with modified methods, only slight reactions were obtained in cat tissues compared to sections of rat organs. Four antibodies against human macrophage antigens were tested in paraffin sections. Only the antibody against alpha-1-antichymotrypsin showed no cross-reactions in the cat. Neutrophilic granulocytes reacted strongly, macrophages only very slight with the anti-lysozyme-antisera and the monoclonal antibody MAC 387. Apart from some macrophages, interdigitating reticulum cells, B- and T-lymphocytes in the cat were stained by the anti HLA-DR antibody TAL-1B5.
Hofmann
C
C
Institut für Tierpathologie, Universität München.
Hermanns
W
W
D000818:Animals; D002415:Cats / Q000276:immunology*; D005260:Female; D006651:Histocytochemistry; D007150:Immunohistochemistry; D007963:Leukocytes, Mononuclear / Q000201:enzymology / Q000276:immunology*; D008297:Male; D010586:Phagocytes / Q000201:enzymology / Q000276:immunology*
Darstellung von Zellen des Mononukleären Phagozytensystems (MPS) der Katze mit Hilfe enzym- und immunhistochemischer Methoden.