title: Expression of glycosylphosphatidylinositol-anchored NAD glycohydrolase in differentiated HL60 cells by phorbol ester.
abstract: Human leukemic HL60 cells are known to express NAD glycohydrolase (NADase) activity following differentiation into macrophage-like cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) or granulocyte-like cells by retinoic acid (RA) treatment. Recently, it was reported that 46 kDa human leukocyte antigen, CD38, expressed by RA-differentiated HL60 cells contained NADase, ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activities. In the present study we questioned whether the NADase activity found in TPA-differentiated HL60 cells is similar to that found in RA-treated cells. Herein we demonstrate that, unlike what is observed following RA treatment, the NADase activity of TPA differentiated cells associates with a 65 kDa glycosylphosphatidylinositol-anchored NADase.
authors.affiliation: Department of Biochemistry, Chonbuk National University Medical School, Chonju, Korea.
authors.forename: M K
authors.initials: MK
authors.lastname: Han
authors.forename: N H
authors.initials: NH
authors.lastname: An
authors.forename: U H
authors.initials: UH
authors.lastname: Kim
mesh_terms: D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000032:analysis / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002462:Cell Membrane / Q000201:enzymology; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute / Q000201:enzymology*; D008562:Membrane Glycoproteins; D009699:N-Glycosyl Hydrolases / Q000032:analysis / Q000378:metabolism; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D014212:Tretinoin; D009699:N-Glycosyl Hydrolases; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D009244:NAD+ Nucleosidase; D051997:ADP-ribosyl Cyclase 1; D013755:Tetradecanoylphorbol Acetate