Acute hypertension induces heat-shock protein 70 gene expression in rat aorta.
BACKGROUND
Many factors cause acute systemic hypertension, which in turn can result in damage to the vessel wall and lead to vascular disease. In previous studies, we demonstrated that restraint, or immobilization stress, results in the induction of heat-shock protein 70 (hsp70) gene expression in the aorta of adult rat and showed that this response was markedly attenuated with age.


RESULTS
Here we provide evidence that restraint-induced hsp70 expression occurs secondary to a rise in systemic blood pressure. Old rats were unable to mount a significant stress-induced hypertensive response, providing an explanation for the reduced hsp70 response in the old rats. A variety of vasoactive agents that induce acute hypertension through distinct signal transduction pathways, including phenylephrine, dopamine, vasopressin, angiotensin II, and endothelin-1, were found to result in hsp70 mRNA induction in the aorta. The magnitude of hsp70 expression achieved with these hypertensive agents was directly correlated with their relative effects on blood pressure. Rats were treated with the vasodilator sodium nitroprusside, which prevented an acute rise in blood pressure from the hypertensive agents tested and abolished induction of hsp70 expression.


CONCLUSIONS
These findings support the conclusion that hsp70 induction occurs as a physiological response to acute hypertension and suggest the possibility that hsp70 plays a role in the protecting the vasculature from damage during hemodynamic stress.
Xu
Q
Q
Section on Gene Expression and Aging, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA.
Li
D G
DG
Holbrook
N J
NJ
Udelsman
R
R
D000375:Aging / Q000378:metabolism / Q000502:physiology; D000818:Animals; D001011:Aorta / Q000378:metabolism; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D006973:Hypertension / Q000188:drug therapy / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012149:Restraint, Physical; D013312:Stress, Physiological / Q000378:metabolism* / Q000503:physiopathology
D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D009599:Nitroprusside