title: A Saccharomyces cerevisiae gene essential for viability has been conserved in evolution.
abstract: To identify the gene coding for the endonuclease which processes the 3' end of mitochondrial (mt) tRNA transcripts in Saccharomyces cerevisiae, nuclear mutations able to complement a mt mutant (Ts932) defective for this process were isolated and analyzed. One of these mutants exhibited a growth defect both on respiratory and fermentable media. Complementation of this phenotype with a S. cerevisiae centrometric wild-type genomic library has allowed us to identify a new essential S. cerevisiae gene strongly conserved in various eukaryotic organisms.
authors.affiliation: Pasteur Institute-Cenci Bolognetti Foundation, Department of Cell and Developmental Biology, University of Rome, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Rinaldi
authors.forename: M
authors.initials: M
authors.lastname: Bolotin-Fukuhara
authors.forename: L
authors.initials: L
authors.lastname: Frontali
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005075:Biological Evolution; D002107:Caenorhabditis / Q000235:genetics; D017124:Conserved Sequence; D004272:DNA, Mitochondrial / Q000378:metabolism; D005800:Genes, Fungal; D006801:Humans; D008969:Molecular Sequence Data; D009115:Muridae; D012349:RNA, Transfer, Asp / Q000096:biosynthesis / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development; D012568:Schizosaccharomyces / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D004272:DNA, Mitochondrial; D012349:RNA, Transfer, Asp