title: Mitochondrial calcium uptake in bovine frozen sperm.
abstract: Mitochondria from bovine frozen semen are capable of preserving oxidative energy. The purpose of this work was to study calcium uptake by the inner mitochondrial membrane which, together with the plasmatic membrane, is involved in spermatic motility and viability regulation. Mitochondrial coupling was confirmed. In the active state, there was a drop in oxygen uptake rate using calcium concentrations ranging from 20 to 100 microM. In the resting state, 75 microM calcium concentration mimicked the active respiration state. In both states, concentrations greater than 100 microM induced mitochondrial uncoupling. Maximal mitochondrial uptake was recorded in the active state with 10 microM lactate. At calcium concentrations greater than 100 microM, there was a drop in mitochondrial calcium uptake for both respiration states. The ability to preserve oxidative energy allows the spermatozoon to take up mitochondrial calcium, thus regulating its functional activity.
authors.affiliation: Cátedra de Química Biológica, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Argentina.
authors.forename: N B
authors.initials: NB
authors.lastname: Beorlegui
authors.forename: M
authors.initials: M
authors.lastname: Córdoba
authors.forename: M T
authors.initials: MT
authors.lastname: Beconi
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D002118:Calcium / Q000493:pharmacokinetics*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D015925:Cryopreservation; D007425:Intracellular Membranes / Q000378:metabolism; D007773:Lactates / Q000494:pharmacology; D008293:Malates / Q000494:pharmacology; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects / Q000502:physiology; D011773:Pyruvates / Q000494:pharmacology; D012662:Semen Preservation; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000378:metabolism*; D013386:Succinates / Q000494:pharmacology
chemical_list: D007773:Lactates; D008293:Malates; D011773:Pyruvates; D013386:Succinates; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000244:Adenosine Diphosphate; D002118:Calcium