Human and mouse dopamine transporter genes: conservation of 5'-flanking sequence elements and gene structures.
Synaptic reaccumulation of the neurotransmitter dopamine is mediated by the dopamine transporter (DAT), a member of the family of twelve transmembrane domain, sodium- and chloride-dependent neurotransmitter transporters. Several DAT features, including its exclusive expression in dopaminergic neurons, implication in cocaine action, and prominent role in the mechanisms of Parkinsonism-inducing neurotoxins, make understanding of the DAT gene of interest. Isolation and characterization of the human and mouse DAT genes has allowed elucidation of similarities between each and other members of this transporter gene family. Sequences 5' to transcriptional start sites contain G-C rich, TATA-less, CAAT-less regions with striking conservation between human and mouse gene flanking regions. These studies suggest sequence elements that are candidates to contribute to the dopamine transporter's dopaminergic cell-specific expression.
Donovan
D M
DM
Molecular Neurobiology Branch, Baltimore, MD 21224, USA.
Vandenbergh
D J
DJ
Perry
M P
MP
Bird
G S
GS
Ingersoll
R
R
Nanthakumar
E
E
Uhl
G R
GR
D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary; D004298:Dopamine / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D050483:Dopamine Plasma Membrane Transport Proteins; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism
D002352:Carrier Proteins; D018076:DNA, Complementary; D050483:Dopamine Plasma Membrane Transport Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; C493214:SLC6A3 protein, human; C493215:Slc6a3 protein, mouse; D004298:Dopamine