Age-related alterations in cell division and cell cycle kinetics in control and trimethyltin-treated lymphocytes of human individuals.
Trimethyltin chloride induced age-related suppression of cell division and cell cycle kinetics in human peripheral blood lymphocytes cultured in RPMI 1640 culture medium supplemented with human AB serum, phytohemagglutinin and bromodeoxyuridine. A high frequency of M1 (first metaphase) cells was seen in cultures treated with a high dose (C1 = 1.0 microgram per culture) and in lymphocytes from donors in the age range 40-70 years. The delay in cell division and cell cycle kinetics may indicate a longer duration in DNA synthesis induced by trimethyltin chloride in aged lymphocytes.
Ganguly
B B
BB
Biochemistry Unit, Indian Statistical Institute, Calcutta.
D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002648:Child; D002675:Child, Preschool; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007223:Infant; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D014298:Trimethyltin Compounds / Q000633:toxicity*
D014298:Trimethyltin Compounds; D000255:Adenosine Triphosphate; D004247:DNA; C040533:trimethyltin chloride