Osteoblastic intracellular pH and calcium in metabolic and respiratory acidosis.
In vitro metabolic acidosis (Met) induces greater bone mineral resorption than respiratory acidosis (Resp). Met, but not Resp, inhibits osteoblasts which control many aspects of osteoclastic function. To determine whether at a similar decrement in extracellular pH, Met and Resp would induce different changes in intracellular pH (pHi) and/or intracellular calcium concentration ([Ca2+]i) of osteoblasts, we measured pHi and [Ca2+]i in an osteoblast-like rat osteosarcoma cell line (UMR-106). Cells were grown to confluence on glass slides and loaded with either 1.5 microM BCECF, for pHi, or 1.5 microM Fura-2, for [Ca2+]i, in control (Ctl; pH approximately 7.40, PCo2 approximately 40, [HCO3-] approximately 24) medium. The fluorescence ratio at excitation wavelengths of 502 and 440 nm was measured for pHi and at 340 and 380 nm for [Ca2+]i. Following a baseline scan in Ctl medium, cells were transferred to either Met (pH approximately 7.10, PCo2 approximately 40, [HCO3-] approximately 12), Resp (pH approximately 7.10, PCo2 approximately 80, [HCO3-] approximately 24) or Ctl conditions. Medium pH, PCo2 and [HCO3-] were held constant over the course of the experiment. Compared to Ctl, pHi was lower in Met (P < 0.001) and even lower in Resp (P < 0.001 vs. Met and vs. Ctl). These changes were maintained over the period of observation. Compared to Ctl, [Ca2+]i was higher in Met (P < 0.001) and even higher in Resp (P < 0.001 vs. Met and vs. Ctl) within 20 to 100 seconds. However, after 100 seconds [Ca2+]i was not different in the three groups.(ABSTRACT TRUNCATED AT 250 WORDS)
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Department of Medicine, University of Rochester School of Medicine and Dentistry, New York, USA.
Lee
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Krieger
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Bushinsky
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D000138:Acidosis / Q000378:metabolism* / Q000473:pathology; D000142:Acidosis, Respiratory / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002460:Cell Line; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism*; D051381:Rats; D013997:Time Factors
D002118:Calcium