title: Molecular cloning and sequence analysis of human preprocathepsin C.
abstract: A cDNA clone (C1) coding for human preprocathepsin C was isolated from a human ileum cDNA library using a rat kidney-derived RT-PCR probe and its complete nucleotide sequence determined. The full-length 1857 bp sequence codes for a protein of 463 amino acid residues with a calculated molecular mass of 51848 Da. Comparison of the deduced amino acid sequence with that of rat preprocathepsin C indicates an 87.5% identity. A multiple alignment of the deduced cathepsin C sequence of 233 residues which, by analogy to other cystein proteinases, corresponds to the mature protein, confirms that human cathepsin C belongs to the papain superfamily.
authors.affiliation: Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, Ljubljana, Slovenia.
authors.forename: A
authors.initials: A
authors.lastname: Paris
authors.forename: B
authors.initials: B
authors.lastname: Strukelj
authors.forename: J
authors.initials: J
authors.lastname: Pungercar
authors.forename: M
authors.initials: M
authors.lastname: Renko
authors.forename: I
authors.initials: I
authors.lastname: Dolenc
authors.forename: V
authors.initials: V
authors.lastname: Turk
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002403:Cathepsins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004792:Enzyme Precursors / Q000737:chemistry / Q000235:genetics*; D018628:Gene Dosage; D005796:Genes / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010206:Papain / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D004792:Enzyme Precursors; D002403:Cathepsins; C095231:preprocathepsin C; D010206:Papain