title: Endogenous interleukin 10 prevents apoptosis in macrophages during Salmonella infection.
abstract: To elucidate the biological roles of endogenous interleukin 10 (IL-10) in macrophage responses during bacterial infection, we examined in vitro effects of neutralizing IL-10 by anti-IL-10 monoclonal antibodies (mAb) on apoptosis of the peritoneal macrophages following Salmonella choleraesuis infection. Marked increments of TNF-alpha production were observed in the culture supernatant later than 6 h after in vitro culture with anti-IL-10 mAb. These macrophages succumbed to apoptosis at this stage accompanied by marked increment of IL-1 release, despite the expression of higher amount of endogenous heat shock protein 70, an inhibitor of TNF-alpha-mediated apoptosis. These results suggest that endogenous IL-10 plays an essential role in protection of Salmonella-infected macrophages from autocrine suicide caused by excessive production of TNF-alpha after killing of Salmonella.
authors.affiliation: Laboratory of Host Defense and Germfree Life, Nagoya University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Arai
authors.forename: K
authors.initials: K
authors.lastname: Hiromatsu
authors.forename: H
authors.initials: H
authors.lastname: Nishimura
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
authors.forename: N
authors.initials: N
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Ishida
authors.forename: Y
authors.initials: Y
authors.lastname: Nimura
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshikai
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D017209:Apoptosis; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000502:physiology*; D017737:Macrophages, Peritoneal / Q000382:microbiology / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D012481:Salmonella Infections, Animal / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D018840:HSP70 Heat-Shock Proteins; D016753:Interleukin-10; D009569:Nitric Oxide