Mutational specificity of the ferrous ion in a supF gene of Escherichia coli.
A plasmid, pZ189, was treated with Fe2+/EDTA, and mutagenesis was determined by DNA sequencing. In the fgp+ Escherichia coli host, 78% were base substitutions, with G:C- > C:G transversion (58.7%) predominant, followed by G:C- > T:A transversion (28.3%). In the fpg-1 mutant, 88% were base substitutions among which 46% were G:C- > C:G and 42% G:C- > T:A. Fe2+ resulted in increased formation of 8-hydroxydeoxyguanosine (8-ohdG) in pZ189 DNA. The origin of Fe(2+)-induced G:C- > T:A transversion may be 8-ohdG; on the other hand, the origin of G:C- > C:G is neither 8-ohdG nor 2,6-diamino-4-hydroxy-5-formamidopyrimidine.
Akasaka
S
S
Osaka Prefectural Institute of Public Health, Japan.
Yamamoto
K
K
D000080242:8-Hydroxy-2'-Deoxyguanosine; D001483:Base Sequence; D004251:DNA Transposable Elements; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D016368:Frameshift Mutation; D005798:Genes, Bacterial; D007501:Iron / Q000494:pharmacology; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids; D017354:Point Mutation; D012680:Sensitivity and Specificity; D017384:Sequence Deletion; D014162:Transfection
D004251:DNA Transposable Elements; D000080242:8-Hydroxy-2'-Deoxyguanosine; D007501:Iron; D003849:Deoxyguanosine