Induction of single strand DNA breaks in workers professionally exposed to polycyclic aromatic hydrocarbons.
An epidemiological study was performed on 42 workers from coke works with the aim to evaluate the usefulness of monitoring single strand DNA breaks (SSBs) in human lymphocytes to assess exposure to polycyclic aromatic hydrocarbons (PAHs) as well as the usefulness of SSBs induction as an indicator of biological effects of PAHs. SSBs can be readily quantitated by a simple fluorometric assay of DNA unwinding. Compared with the control group, statistically significant increase in SSBs was observed in coke oven workers occupationally exposed to PAHs. These findings are in agreement with previous results obtained by a different method of measuring DNA damage in subjects exposed to high PAH levels. The findings confirmed that SSBs determination in human lymphocytes reflected the exposure to PAHs and the FADU method appears to be useful in the revelation of effects of occupational exposure to industrial air pollutants such as PAHs.
Salagovic
J
J
Institute of Medical Biology, School of Medicine, P. J. Safárik University, Kosice, Slovakia.
Kalina
I
I
Dubayová
K
K
D003077:Coke; D004249:DNA Damage; D004277:DNA, Single-Stranded; D005069:Evaluation Studies as Topic; D005470:Fluorometry; D006801:Humans; D007221:Industry; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D016273:Occupational Exposure; D011083:Polycyclic Compounds / Q000009:adverse effects*
D003077:Coke; D004277:DNA, Single-Stranded; D011083:Polycyclic Compounds