Identification of the cis-elements required for transcriptional control of the Chinese hamster ovary APRT gene.
Transcriptional regulation of the Chinese hamster ovary (CHO) adenine phosphoribosyltransferase-encoding gene (APRT) was studied. The 5' region of the CHO APRT is G + C-rich, but lacking TATA or CCAAT boxes. RNase protection assays indicate that it contains multiple transcription start points (tsp). A tsp 64 bp upstream from the translation start codon is denoted as +1. Linker-scanning (LS) mutation analysis indicates that the -33 to +19 region is important in regulating APRT transcription. Mutations in the -23 to -14 region abolish transcription initiated from the -23 downstream region. An unidentified protein complex binds to this region. Three Sp1-binding sites are found in the APRT promoter; however, mutations of the Sp1-binding sites do not reduce APRT transcription. Mutations at two putative GCF-binding sites increase levels of transcription.
She
B R
BR
Department of Biology, Indiana University, Bloomington 47405, USA.
Taylor
M W
MW
D000228:Adenine Phosphoribosyltransferase / Q000096:biosynthesis / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D016466:CHO Cells; D006224:Cricetinae; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; D016329:Sp1 Transcription Factor; D000228:Adenine Phosphoribosyltransferase