title: Duplication of the MHC-linked Xenopus complement factor B gene.
abstract: We have previously reported the molecular cloning of the mammalian major histocompatibility complex (MHC) class III gene, complement factor B (Bf) from Xenopus laevis, and linkage of the gene to the frog MHC. Here, we estimated the copy number of the Xenopus Bf gene by genomic Southern blotting analysis and demonstrated that Xenopus laevis has two copies of the Bf gene. Both genes co-segregated with the MHC-linked HSP70 genes among 19 offspring of an f/r x f/r cross, indicating a close linkage of the two Bf genes to the frog MHC. Both genes are transcribed and contain open reading frames. When compared with the previously determined cDNA sequence (Xenopus Bf A), the predicted amino acid sequence of the second cDNA species (Xenopus Bf B) shows 82% overall identity. Polymerase chain reaction analysis indicated that all of the partially inbred frogs with the f, r, g, and j MHC haplotypes, as well as 12 outbred frogs tested have both Bf genes, suggesting that the duplicated Bf genes are stable genetic traits in Xenopus laevis.
authors.affiliation: Department of Biochemistry, Nagoya City University Medical School, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kato
authors.forename: L
authors.initials: L
authors.lastname: Salter-Cid
authors.forename: M F
authors.initials: MF
authors.lastname: Flajnik
authors.forename: C
authors.initials: C
authors.lastname: Namikawa
authors.forename: M
authors.initials: M
authors.lastname: Sasaki
authors.forename: M
authors.initials: M
authors.lastname: Nonaka
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D011415:Complement Factor B / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D008040:Genetic Linkage; D008285:Major Histocompatibility Complex / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014982:Xenopus laevis / Q000235:genetics* / Q000276:immunology
chemical_list: D018076:DNA, Complementary; D011415:Complement Factor B