Sequences within the parvovirus minute virus of mice NS2-specific exon are required for inclusion of this exon into spliced steady-state RNA.
When the minute virus of mice NS2-specific exon was modified by either substitution or deletion, most P4-generated pre-mRNA was spliced from the large-intron donor at nucleotide 514 to the small-intron acceptor at nucleotide 2377. Improvement to consensus of large-intron splice sites in such mutants did not suppress exon skipping or restore large-intron excision. Therefore, sequences within the NS2-specific exon are required for inclusion of this exon into spliced, steady-state minute virus of mice RNA.
Zhao
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Q
Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, Columbia 65212, USA.
Mathur
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S
Burger
L R
LR
Pintel
D J
DJ
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D016384:Consensus Sequence; D004285:Dogs; D005091:Exons; D007438:Introns; D007668:Kidney; D008915:Minute Virus of Mice / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis; D017384:Sequence Deletion; D014158:Transcription, Genetic; D014162:Transfection; D017361:Viral Nonstructural Proteins / Q000096:biosynthesis / Q000235:genetics*
D012322:RNA Precursors; D012333:RNA, Messenger; D012367:RNA, Viral; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins