Temporal relationships and IL-2 dependency of prolactin-induced lymphocyte proliferation.
Recombinant preparations of human prolactin (hPRL) and interleukin 2 (hIL-2) as well as monoclonal antibodies to these growth factors were used to study the synergistic interaction of PRL and IL-2 in Nb2 rat lymphoma lactogen-dependent cells. It was shown that IL-2 stimulated Nb2 cell proliferation in lactogen-free culture medium. Experiments with short-term exposure to growth factor demonstrated that PRL was required only during the initial 12 h of incubation while IL-2 was mitogenic regardless of the time it was added. Antibody to IL-2 partially inhibited both PRL- and IL-2-induced proliferation whereas antibody to PRL significantly decreased PRL but not IL-2-induced proliferation. These findings suggest that the complete mitogenic effect of PRL on Nb2 cells requires stimulation of IL-2 production.
Gushchin
G V
GV
Department of Internal Medicine, Ohio State University Medical Center, Columbus 43210, USA.
Cheney
C
C
Glaser
R
R
Malarkey
W B
WB
D000818:Animals; D000906:Antibodies / Q000276:immunology; D004357:Drug Synergism; D007376:Interleukin-2 / Q000276:immunology / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects*; D011388:Prolactin / Q000276:immunology / Q000494:pharmacology*; D051381:Rats; D013997:Time Factors; D014407:Tumor Cells, Cultured
D000906:Antibodies; D007376:Interleukin-2; D011388:Prolactin