Purification and N-terminal sequence of a novel interferon-induced 100 kD protein secreted by mouse Ehrlich ascites tumor cells.
Mouse Ehrlich ascites tumor cells secrete a 100 kD protein when they are stimulated by murine interferon-beta (2000 IU/ml). This 100 kD protein was purified from conditioned medium by chromatography on phenyl-Sepharose CL-4B, phosphocellulose, DEAE-Sephacel, and Vydac C4 columns and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A single species of 100 kD protein was isolated, and its N-terminal sequence analysis suggested that mouse Ehrlich ascites tumor cell-derived interferon-induced 100 kD protein is a novel protein.
Tominaga
S
S
Department of Biochemistry II, Jichi Medical School, Tochigi, Japan.
Utsumi
J
J
D000595:Amino Acid Sequence; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D017077:Culture Media, Conditioned; D004591:Electrophoresis, Polyacrylamide Gel; D016899:Interferon-beta / Q000494:pharmacology*; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D009363:Neoplasm Proteins; D011994:Recombinant Proteins; D016899:Interferon-beta