title: ESR studies on the effect of ionic radii on displacement of Mn2+ bound to a soluble beta-galactoside binding hepatic lectin.
abstract: Binding of divalent metal ions to hepatic soluble beta-galactoside binding lectin was studied using electron spin resonance (ESR) spectroscopy. The Mn2+ bound to hepatic lectin could be displaced by Mg2+, Cu2+, Ni2+ and Ca2+ but not by Sr2+. As the ionic radii of Mg2+ (0.65 A), Cu2+ (0.73 A) and Ni2+ (0.72 A) are appreciably smaller than Ca2+ (0.99 A), it appears that the Mn2+ binding site is more accessible to Mg2+, Cu2+, and Ni2+ as compared to Ca2+, the ionic radius of Mn2+ being 0.80 A. Sr2+ with an ionic radius of 1.13 is thus unable to displace bound Mn2+. Surprisingly, the presence of specific sugars like alpha-lactose, or alpha-D-galactose facilitated the displacement of bound Mn2+ by metal ions whereas non-specific sugars, i.e. alpha-D-glucose, beta-D-fructose and alpha-D-ribose had no effect. It appears that minor perturbations in the saccharide binding site significantly affect the ability of the metal binding site to ligate bivalent metals.
authors.affiliation: School of Life Sciences, Devi Ahilya Vishwavidyyalaya, Vigyan Bhawan, Indore, India.
authors.forename: R
authors.initials: R
authors.lastname: Kayestha
authors.lastname: Sumati
authors.forename: K
authors.initials: K
authors.lastname: Hajela
mesh_terms: D000818:Animals; D001665:Binding Sites; D002413:Cations, Divalent; D004578:Electron Spin Resonance Spectroscopy; D005690:Galactose / Q000378:metabolism; D037161:Galectins; D006041:Goats; D006388:Hemagglutinins / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D007785:Lactose / Q000378:metabolism; D008099:Liver / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002413:Cations, Divalent; D037161:Galectins; D006388:Hemagglutinins; D008345:Manganese; D007785:Lactose; D005690:Galactose