Effect of TJ-96, an anti-allergic herbal medicine, on tracheal transepithelial potential difference in vivo.
We studied the effect of Saiboku-to (TJ-96), an anti-allergic herbal medicine, on transepithelial potential difference of rabbit trachea and possible involvement of nitric oxide (NO) generation in vivo. Perfusion of TJ-96 on the tracheal mucosal surface increased PD in a concentration-dependent manner, the maximal increase from the baseline level and the concentration of TJ-96 required to produce a half-maximal effect (EC50) being 8.1 +/- 1.4 mV (mean +/- SE, P < 0.001) and 47 micrograms/ml. This effect was abolished by pretreatment with the Na channel blocker amiloride. NG-nitro-L-arginine methylester (L-NAME) but not NG-nitro-D-arginine methylester (D-NAME) inhibited TJ-96-induced increase in PD, and this inhibition was selectively reversed by L-arginine. These results suggest that TJ-96 stimulates Na absorption by airway epithelial cells probably through NO generation.
Tamaoki
J
J
First Department of Medicine, Tokyo Women's Medical College, Japan.
Takemura
H
H
Kondo
M
M
Chiyotani
A
A
Sakai
A
A
Konno
K
K
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004848:Epithelium / Q000187:drug effects / Q000502:physiology; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006967:Hypersensitivity / Q000188:drug therapy*; D008297:Male; D020835:Medicine, Kampo; D008564:Membrane Potentials / Q000187:drug effects; D009092:Mucous Membrane / Q000378:metabolism; D019331:NG-Nitroarginine Methyl Ester; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D011817:Rabbits; D012964:Sodium / Q000378:metabolism; D014132:Trachea / Q000187:drug effects* / Q000502:physiology
D004365:Drugs, Chinese Herbal; D006634:Histamine H1 Antagonists; C045086:saiboku-to; D009569:Nitric Oxide; D000584:Amiloride; D001120:Arginine; D012964:Sodium; D019331:NG-Nitroarginine Methyl Ester