title: Adducin: a physical model with implications for function in assembly of spectrin-actin complexes.
abstract: Adducin binds to spectrin-actin complexes, promotes association of spectrin with actin, and is subject to regulation by calmodulin as well as protein kinases A and C. Adducin is a heteromer comprised of homologous alpha and beta-subunits with an NH2-terminal protease-resistant head domain, connected by a neck region to a COOH-terminal hydrophilic, protease-sensitive region. This study provides evidence that adducin in solution is a mixture of heterodimers and tetramers. CD spectroscopy of COOH-terminal domains of alpha- and beta-adducin bacterial recombinants provides direct evidence for an unstructured random coil configuration. Cross-linking, proteolysis, and blot-binding experiments suggest a model for the adducin tetramer in which four head domains contact one another to form a globular core with extended interacting alpha- and beta-adducin tails. The site for binding to spectrin-actin complexes on adducin was identified as the COOH-terminal tail of both the alpha- and beta-adducin subunits. The capacity of native adducin to recruit spectrin to actin filaments is similar to that of adducin tail domains. Thus, adducin tail domains alone are sufficient to interact with F-actin and a single spectrin and to recruit additional spectrin molecules to the ternary complex.
authors.affiliation: Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Hughes
authors.forename: V
authors.initials: V
authors.lastname: Bennett
mesh_terms: D000199:Actins / Q000737:chemistry*; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry*; D002148:Calmodulin-Binding Proteins / Q000737:chemistry*; D006801:Humans; D008961:Models, Structural; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry; D013049:Spectrin / Q000737:chemistry*; D000099291:Adducins
chemical_list: D000199:Actins; D001798:Blood Proteins; D002148:Calmodulin-Binding Proteins; D011994:Recombinant Proteins; D013049:Spectrin; D000099291:Adducins