Isolation and characterization of a cDNA encoding for preprosomatostatin containing [Tyr7, Gly10]-somatostatin-14 from the endocrine pancreas of rainbow trout, Oncorhynchus mykiss.
Somatostatins are a diverse group of peptides known to influence various aspects of growth and metabolism of vertebrates. In order to further our understanding of the physiological roles of somatostatins in fish, we initiated an analysis of somatostatin gene structure and expression in rainbow trout pancreas. Using rapid amplification of cDNA ends polymerase chain reaction, we have isolated, cloned, and sequenced a novel cDNA derived from pancreatic total RNA. Sequence analysis revealed a 624-bp cDNA containing the complete 5'-untranslated region with a single initiation site 107 bases from the most 5' end and a single putative polyadenylation site 13 bases from the most 3' end that was terminated with a polyadenylated tail. The deduced protein is a 115-amino-acid preprosomatostatin molecule with [Tyr7,Gly10]-somatostatin-14 at the C-terminus of the coding region, making the rainbow trout precursor a member of the preprosomatostatin II family. Based on the location of putative cleavage sites, we propose that rainbow trout pancreatic preprosomastatin II is processed to yield a 28-amino-acid and/or, possibly, a 14-amino-acid somatostatin II molecule. The results also suggest that there has been limited conservation of the preprosomatostatin II gene family among teleosts.
Moore
C A
CA
Department of Zoology, North Dakota State University, Fargo 58105, USA.
Kittilson
J D
JD
Dahl
S K
SK
Sheridan
M A
MA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification*; D007515:Islets of Langerhans / Q000737:chemistry*; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D017385:Sequence Homology; D013004:Somatostatin / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D011498:Protein Precursors; D013004:Somatostatin