Strategies for the synthesis and screening of glycoconjugates. 2. Covalent immobilization for flow cytometry.
Glycosylamines are readily available carbohydrate derivatives that undergo acylation reactions with homobifunctional N-hydroxysuccinimidyl esters. The product glycosylamides carry a spacer group equipped with one active ester functionality. This route provides well-defined glycoconjugates, which may be cross-linked to various amino-functionalized resins. Carbohydrate recognition of the resulting sugar-bead conjugates is probed by lectin immunostaining or flow cytometry using a fluorescently labeled lectin.
Vetter
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Affymax Research Institute, Palo Alto, California 94304, USA.
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Gallop
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D002240:Carbohydrate Sequence; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006001:Glycoconjugates / Q000032:analysis / Q000138:chemical synthesis*; D037102:Lectins / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D016339:Spectrometry, Mass, Fast Atom Bombardment
D005456:Fluorescent Dyes; D006001:Glycoconjugates; D037102:Lectins