Purification of sex pheromones specific for pMB1 and pMB2 plasmids of Enterococcus faecalis S-48.
The strain Enterococcus faecalis S-48 carries two large conjugative plasmids (pMB1 and pMB2) encoding for antagonistic substances. The pheromone response of these two plasmids was established by purifying the corresponding sex pheromones, using conventional reversed-phase columns. Plasmid pMB1 responds to pheromone cCF10. Plasmid pMB2 responds to a pheromone with an amino acid sequence identical to that of cPD1 (Phe-Leu-Val-Met-Phe-Leu-Ser-Gly). The two pheromone-responding plasmids coexist in a stable fashion in the wild-type strain E. faecalis S-48.
Quirantes
R
R
Department of Microbiology, Faculty of Sciences, University of Granada, Spain.
Gálvez
A
A
Valdivia
E
E
Martín
I
I
Martínez-Bueno
M
M
Méndez
E
E
Maqueda
M
M
D000595:Amino Acid Sequence; D001422:Bacterial Adhesion / Q000187:drug effects; D003227:Conjugation, Genetic / Q000187:drug effects* / Q000235:genetics; D013293:Enterococcus faecalis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000502:physiology*; D017421:Sequence Analysis; D012724:Sex Attractants / Q000302:isolation & purification* / Q000494:pharmacology
D012724:Sex Attractants