title: Relationship of nucleolus-associated bodies with the nucleolar organizer tracks in plant interphase nuclei (Pisum sativum).
abstract: Nucleolus-associated bodies (NABs) have long been noted in interphase nuclei of a wide variety of plant species. We have recently shown that these bodies consist largely of snRNPs and that they are located on the nucleolar surface in the immediate vicinity of the nucleolar organizer tracks. The present study revealed that, following exposure of roots to KCN, an agent that induces nucleolar segregation, NABs were intimately associated with intranucleolar chromatin. Although immunocytochemical tests with anti-DNA indicated that NABs contained no demonstrable amounts of DNA, our observations nevertheless add further support to the notion that these bodies are somehow related to the nucleolar chromosomes.
authors.affiliation: Département de Biologie, Université Laval, Québec, Canada.
authors.forename: J G
authors.initials: JG
authors.lastname: Lafontaine
authors.forename: H
authors.initials: H
authors.lastname: Chamberland
mesh_terms: D002466:Cell Nucleolus / Q000187:drug effects / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure; D002875:Chromosomes / Q000187:drug effects / Q000648:ultrastructure; D018744:DNA, Plant / Q000032:analysis / Q000187:drug effects; D007399:Interphase; D008854:Microscopy, Electron; D009697:Nucleolus Organizer Region / Q000187:drug effects / Q000648:ultrastructure*; D018532:Pisum sativum / Q000187:drug effects / Q000648:ultrastructure*; D018517:Plant Roots / Q000187:drug effects / Q000648:ultrastructure; D011190:Potassium Cyanide / Q000494:pharmacology; D017411:Ribonucleoproteins, Small Nuclear / Q000032:analysis / Q000187:drug effects / Q000648:ultrastructure*
chemical_list: D002843:Chromatin; D018744:DNA, Plant; D017411:Ribonucleoproteins, Small Nuclear; D011190:Potassium Cyanide