Localization of immuno-analogues of erythrocyte protein 4.1 and spectrin in epidermis of psoriasis vulgaris.
The presence and localization of immuno-analogues of human erythrocyte protein 4.1 and spectrin were examined in the epidermis of psoriasis vulgaris. Immunoblot analysis with antibodies against human erythrocyte protein 4.1 revealed that psoriatic epidermis contains a 4.1-like protein of 80 kDa, and also minor immunoreactive polypeptides, including a 45-kDa polypeptide. The 45-kDa band was not detected in non-lesional epidermis. Lesional epidermis of psoriasis contains spectrin-like proteins of 240 kDa. Analysis with immunofluorescence microscopy revealed that 4.1-like proteins were detected mainly in the cytoplasm of the suprabasal cells in lesional epidermis and in the peripheral cytoplasm of the basal cells in non-lesional epidermis. On the other hand, spectrin-like proteins were localized to the peripheral cytoplasm of basal keratinocytes in both lesional and non-lesional psoriatic epidermis. The present results indicate that proteins related to protein 4.1 and spectrin are consistently detected within epidermal cells of psoriasis, a chronic skin disease characterized by epidermal hyperplasia; the expression and distribution of protein 4.1 in lesional epidermis of psoriasis differs from that in non-lesional epidermis. These membrane skeletal proteins may be of significance in the hyperproliferative epidermis of psoriasis.
Shimizu
T
T
Department of Dermatology, Hokkaido University School of Medicine, Sapporo, Japan.
Takakuwa
Y
Y
Koizumi
H
H
Ishibashi
T
T
Ohkawara
A
A
D000328:Adult; D000368:Aged; D003598:Cytoskeletal Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D004817:Epidermis / Q000737:chemistry*; D004910:Erythrocyte Membrane / Q000737:chemistry; D006801:Humans; D015151:Immunoblotting; D015603:Keratinocytes / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis* / Q000276:immunology; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009479:Neuropeptides; D011565:Psoriasis / Q000378:metabolism*; D013049:Spectrin / Q000032:analysis* / Q000276:immunology
D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D009479:Neuropeptides; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1; D013049:Spectrin