Effect of dietary NaCl on tyrosine hydroxylase in the superior cervical ganglia of Dahl rats.
To investigate the involvement of peripheral catecholamines in the development of Dahl-Iwai salt-sensitive (DIS/Eis) hypertension, we performed immunohistochemical staining of tyrosine hydroxylase (TH) in the superior cervical ganglia (SCG) of DIS/Eis rats and Dahl-Iwai salt-resistant (DIR/Eis) rats, and in situ hybridization histochemistry for demonstration of TH mRNA localization in the SCG of these rats. DIS/Eis and DIR/Eis rats were fed on a high (8%) salt diet or on a low (0.3%) salt diet for 4 weeks. Nerve cells in the SCG of DIS/Eis high salt rats exhibited more intense TH-immunoreactivity (P < 0.01) and hybridization signals (P < 0.01) than those of the other experimental groups. These findings suggest that activation of peripheral sympathetic nerves may account for hypertension in DIS/Eis rats on a high salt diet.
Nagae
A
A
Second Department of Internal Medicine, Ehime University School of Medicine, Japan.
Kohara
K
K
Iwata
T
T
Matsuda
S
S
Sakanaka
M
M
Hiwada
K
K
D000818:Animals; D006973:Hypertension / Q000139:chemically induced; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D017673:Sodium Chloride, Dietary / Q000494:pharmacology*; D017783:Superior Cervical Ganglion / Q000187:drug effects* / Q000201:enzymology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D017673:Sodium Chloride, Dietary; D014446:Tyrosine 3-Monooxygenase