Selective separation of human peripheral platelets, granulocytes and lymphocytes by surface affinity chromatography.
Selective separation of human peripheral platelets, granulocytes and lymphocytes was investigated by column liquid chromatography using methoxyethoxymethyl (MEM) bonded-phase columns (25 x 0.9 cm I.D.). Isotonic solutions containing mono- and disaccharides, methyl-alpha-D-pyranosides and a physiological saline at pH 7.4 were used as the mobile phase. Granulocytes and lymphocytes were separated on the MEM-Cellulofine GH-25 column by elution with 0.3 M D-mannose solution. The isolation of platelets and lymphocytes from human leukocyte-rich plasma was performed with a MEM-Sephadex G25 column and elution with 0.27 M sucrose solution. On the same column platelets could also be collected selectively by elution with 0.31 M methyl-alpha-D-mannoside at the high recovery of 100%. The isolated cells were viable for more than 90%.
Shibusawa
Y
Y
Department of Analytical Chemistry, Tokyo College of Pharmacy, Japan.
Suzuki
K
K
Kinoshita
H
H
Matsumoto
U
U
D001792:Blood Platelets / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D002846:Chromatography, Affinity / Q000379:methods*; D002852:Chromatography, Ion Exchange; D006098:Granulocytes / Q000166:cytology*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*