title: Diffuse vesicular distribution of Rab3D in the polarized neuroendocrine cell line AtT-20.
abstract: The neuroendocrine cell line AtT-20 has two types of storage vesicles: dense core granules and synaptic vesicles, both sequestered at the tip of the processes. Here we show that Rab3D protein, which is abundant in fat cells, is also expressed in AtT-20 cells. Differently from Rab3A, which is localized in secretory vesicles accumulated at the tips, Rab3D has a diffuse vesicular distribution in the cytoplasm of the cell body, the processes and the tips. In AtT-20 cells, Rab3D may define a regulated secretory pathway which functions independently from cell polarity.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Martelli
authors.forename: R
authors.initials: R
authors.lastname: Bareggi
authors.forename: G
authors.initials: G
authors.lastname: Baldini
authors.forename: P E
authors.initials: PE
authors.lastname: Scherer
authors.forename: H F
authors.initials: HF
authors.lastname: Lodish
authors.forename: G
authors.initials: G
authors.lastname: Baldini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D003593:Cytoplasm / Q000737:chemistry*; D019204:GTP-Binding Proteins / Q000032:analysis*; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009490:Neurosecretory Systems / Q000737:chemistry* / Q000166:cytology; D020692:rab3 GTP-Binding Proteins
chemical_list: D009419:Nerve Tissue Proteins; D019204:GTP-Binding Proteins; D020692:rab3 GTP-Binding Proteins