Dimethyl sulphoxide alters mitogen induced production of diacylglycerol and GTP-binding to plasma membranes in mouse embryo fibroblasts.
Dimethyl sulphoxide (DMSO) is a polar solvent often used as an inducer of erythroid differentiation. In order to elucidate the mode of action of DMSO, we studied its effects on two early events in signal transduction, namely diacylglycerol (DAG) production and guanosine triphosphate (GTP) binding. Mouse embryo fibroblasts showed a bimodal profile of diacylglycerol synthesis in the presence of DMSO. Platelet derived growth factor (PDGF) mediated synthesis of diacylglycerol synthesis was inhibited by DMSO. Also DMSO down regulated serum induced GTP-binding to isolated plasma membranes of mouse embryo fibroblasts. However, the control and the ras (T24) oncogene transformed Rat-2 cells did not show any increase in GTP-binding on serum stimulation while showing a marginal decrease in GTP-binding in the presence of DMSO. DMSO may hence act by inhibiting the perpetuation of mitogenic signal transduction at an early stage.
Ganapathy
P
P
Cancer Biology Division, School of Biological Sciences, Madurai Kamaraj University, India.
Shanmugam
G
G
D000818:Animals; D002455:Cell Division / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004075:Diglycerides / Q000096:biosynthesis* / Q000235:genetics; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D015536:Down-Regulation; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism; D011905:Genes, ras / Q000502:physiology; D006160:Guanosine Triphosphate / Q000235:genetics / Q000378:metabolism*; D007328:Insulin / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*
C011439:1,2-diacylglycerol; D003470:Culture Media; D004075:Diglycerides; D007328:Insulin; D010982:Platelet-Derived Growth Factor; D006160:Guanosine Triphosphate; D004121:Dimethyl Sulfoxide