title: The effect of n-3 fatty acids on neutrophil chemiluminescence.
abstract: The effect of dietary supplementation with n-3 polyunsaturated fatty acids (PUFAs) on the production of free oxygen radicals from activated neutrophils was investigated in healthy subjects, using chemiluminescence. In the first study 22 persons were give 4 g n-3 PUFAs daily for 6 weeks. There was a median reduction of chemiluminescence from neutrophils stimulated with opsonized zymosan of 37% (p < 0.001). The median content of eicosapentaenoic acid in platelets, used as an indicator for cellular fatty acid profile, increased from 0.70 to 2.80% (p < 0.001), and there was a significant negative correlation between the chemiluminescence signal and the content of eicosapentaenoic acid in platelets (p < 0.001). In a second, low-dose study 24 persons were allocated to daily supplementation with either 0.65 g n-3 PUFAs or with a control oil for 6 weeks. Compared to the control group there was a median reduction of 38 and 44% in chemiluminescence from neutrophils stimulated with opsonized zymosan and phorbol,12-myristate,13-acetate (PMA), respectively. Neither of these differences, however, was statistically significant. These findings lend support for a possible role of n-3 PUFAs in the management of inflammatory disorders.
authors.affiliation: Department of Clinical Immunology, Aalborg Hospital, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Varming
authors.forename: E B
authors.initials: EB
authors.lastname: Schmidt
authors.forename: N
authors.initials: N
authors.lastname: Svaneborg
authors.forename: J M
authors.initials: JM
authors.lastname: Møller
authors.forename: H H
authors.initials: HH
authors.lastname: Lervang
authors.forename: N
authors.initials: N
authors.lastname: Grunnet
authors.forename: C
authors.initials: C
authors.lastname: Jersild
authors.forename: J
authors.initials: J
authors.lastname: Dyerberg
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000737:chemistry; D015118:Eicosapentaenoic Acid / Q000097:blood; D005231:Fatty Acids, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D005395:Fish Oils / Q000008:administration & dosage; D005609:Free Radicals / Q000097:blood; D006801:Humans; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D018375:Neutrophil Activation; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D016897:Respiratory Burst / Q000502:physiology
chemical_list: D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D005609:Free Radicals; D017382:Reactive Oxygen Species; D015118:Eicosapentaenoic Acid