Differential expression of voltage-activated Na+ currents in two prostatic tumour cell lines: contribution to invasiveness in vitro.
The voltage-gated ionic currents of two rodent prostatic cancer cell lines were investigated using the whole-cell patch clamp technique. The highly metastatic Mat-Ly-Lu cells expressed a transient, inward Na+ current (blocked by 600 nM tetrodotoxin), which was not found in any of the weakly metastatic AT-2 cells. Although both cell lines expressed a sustained, outward K+ current, this occurred at a significantly higher density in the AT-2 than in the Mat-Ly-Lu cells. Incubation of the Mat-Ly-Lu cell line with 600 nM tetrodotoxin significantly reduced the invasive capacity of the cells in vitro. Under identical conditions, tetrodotoxin had no effect on the invasiveness of the AT-2 cells.
Grimes
J A
JA
Imperial College of Science, Technology and Medicine, Department of Biology, London, UK.
Fraser
S P
SP
Stephens
G J
GJ
Downing
J E
JE
Laniado
M E
ME
Foster
C S
CS
Abel
P D
PD
Djamgoz
M B
MB
D000818:Animals; D004847:Epithelial Cells; D015640:Ion Channel Gating / Q000187:drug effects; D008297:Male; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D015222:Sodium Channels; D013779:Tetrodotoxin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D015222:Sodium Channels; D013779:Tetrodotoxin