title: Eukaryotic methionyl aminopeptidases: two classes of cobalt-dependent enzymes.
abstract: Using partial amino acid sequence data derived from porcine methionyl aminopeptidase (MetAP; methionine aminopeptidase, peptidase M; EC 3.4.11.18), a full-length clone of the homologous human enzyme has been obtained. The cDNA sequence contains 2569 nt with a single open reading frame corresponding to a protein of 478 amino acids. The C-terminal portion representing the catalytic domain shows limited identity with MetAP sequences from various prokaryotes and yeast, while the N terminus is rich in charged amino acids, including extended strings of basic and acidic residues. These highly polar stretches likely result in the spuriously high observed molecular mass (67 kDa). This cDNA sequence is highly similar to a rat protein, termed p67, which was identified as an inhibitor of phosphorylation of initiation factor eIF2 alpha and was previously predicted to be a metallopeptidase based on limited sequence homology. Model building established that human MetAP (p67) could be readily accommodated into the Escherichia coli MetAP structure and that the Co2+ ligands were fully preserved. However, human MetAP was found to be much more similar to a yeast open reading frame that differed markedly from the previously reported yeast MetAP. A similar partial sequence from Methanothermus fervidus suggests that this p67-like sequence is also found in prokaryotes. These findings suggest that there are two cobalt-dependent MetAP families, presently composed of the prokaryote and yeast sequences (and represented by the E. coli structure) (type I), on the one hand, and by human MetAP, the yeast open reading frame, and the partial prokaryotic sequence (type II), on the other.
authors.affiliation: Department of Biological Chemistry, College of Medicine, University of California, Irvine 92717-1700, USA.
authors.forename: S M
authors.initials: SM
authors.lastname: Arfin
authors.forename: R L
authors.initials: RL
authors.lastname: Kendall
authors.forename: L
authors.initials: L
authors.lastname: Hall
authors.forename: L H
authors.initials: LH
authors.lastname: Weaver
authors.forename: A E
authors.initials: AE
authors.lastname: Stewart
authors.forename: B W
authors.initials: BW
authors.lastname: Matthews
authors.forename: R A
authors.initials: RA
authors.lastname: Bradshaw
mesh_terms: D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000737:chemistry* / Q000145:classification / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003035:Cobalt / Q000494:pharmacology*; D017931:DNA Primers; D018076:DNA, Complementary; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D063208:Methionyl Aminopeptidases; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013045:Species Specificity; D013552:Swine
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D011994:Recombinant Proteins; D003035:Cobalt; D000626:Aminopeptidases; D063208:Methionyl Aminopeptidases