A murine antibody to Shigella dysenteriae type 1 employs V-genes that contain a rearranged codon for the lambda light chain.
The cDNA coding for a hybridoma anti Shigella dysenteriae type 1 antibody (3707 E9) has been cloned, and sequenced. Binding patterns with fragments of the bacterial polysaccharide antigen had already been studied in detail. The VH sequence utilizes the VH441 gene, first identified amongst beta-(1,6)galactan-binding antibodies, while the VL is closely related to the V lambda 1 gene. We found that the VL3707 E9 gene employed a VL-J combinatorial joining leading to a rare Trp-->Leu substitution at position L96.
Miller
C E
CE
Laboratory of Medicinal Chemistry, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA.
Huppi
K
K
Siwarski
D
D
Karpas
A
A
Newman
A
A
Mainhart
C
C
Glaudemans
C P
CP
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000235:genetics*; D000911:Antibodies, Monoclonal / Q000235:genetics*; D001483:Base Sequence; D003062:Codon; D017931:DNA Primers / Q000737:chemistry; D005803:Genes, Immunoglobulin; D006825:Hybridomas; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007146:Immunoglobulin lambda-Chains / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D017354:Point Mutation; D012762:Shigella dysenteriae / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D003062:Codon; D017931:DNA Primers; D007135:Immunoglobulin Variable Region; D007146:Immunoglobulin lambda-Chains