Mapping cells and sub-cellular organelles on 2-D gels: 'new tricks for an old horse'.
Nowadays, investigators in all fields are faced with the identification of unknown, up- or down-regulated, modified proteins that they are trying to identify. Two-dimensional (2-D) gel electrophoresis, with its ability to resolve several thousand proteins, is an extremely powerful technique. The current resolution and reproducibility of 2-D gel technology and the establishment of computer assisted 2-D gel protein databases have paved new ways for the identification of proteins.
Huber
L A
LA
Départment de Biochimie, Université de Genève, Switzerland.
D002458:Cell Fractionation; D002477:Cells / Q000737:chemistry; D016208:Databases, Factual; D015180:Electrophoresis, Gel, Two-Dimensional; D015388:Organelles / Q000737:chemistry; D011506:Proteins / Q000145:classification / Q000302:isolation & purification*
D011506:Proteins