Production of human immunodeficiency virus by chronically infected cells grown in protein-free medium.
A human T cell line chronically infected with Human Immunodeficiency Virus (HIV) has been adapted to grow in a chemically defined, protein-free medium. Virus particles are produced at rates comparable to those of serum-supplemented cultures; virus preparations free of undesirable proteins can be produced in preparative amounts by simple ultrafiltration procedures and cell culture supernatants can be used as such for the preparation of ELISA solid phases. This material has been used very conveniently for studies concerning characterization of antibodies against HIV-specific proteins, interaction of HIV with complement components and inclusion of human cell-derived proteins into virions; we propose its use as a powerful tool for the structural as well as functional analysis of the virus particle itself.
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Dipartimento di Biologia e Genetica per le Scienze Mediche, Università degli Studi di Milano, Italy.
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D000911:Antibodies, Monoclonal / Q000276:immunology; D002478:Cells, Cultured; D003470:Culture Media; D016895:Culture Media, Serum-Free; D006678:HIV / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology*; D014764:Viral Proteins / Q000032:analysis
D000911:Antibodies, Monoclonal; D003470:Culture Media; D016895:Culture Media, Serum-Free; D014764:Viral Proteins