Different patterns of HIV-1-specific cytotoxic T-lymphocyte activity after primary infection.
OBJECTIVE
To analyse the HIV-1-specific cytotoxic T-lymphocyte (CTL) responses of nine HIV-seropositive subjects in relation with primary infection.


METHODS
Anti-HIV CTL were generated by in vitro stimulation of peripheral mononuclear cells obtained from HIV-seropositive donors at various times after primary infection. They were tested against several structural or regulatory HIV-1 proteins, using autologous target cells infected with recombinant vaccinia viruses expressing one of the HIV-1LAI proteins.


RESULTS
An important CTL activity was found during the first month following seroconversion only in those donors who showed clinical symptoms during primary infection. The temporal evolution of this response differed for each subject; one remained a non-responder even 30 months after seroconversion. The structural proteins were recognized particularly early, while the antigenicity of regulatory proteins appeared later.


CONCLUSIONS
Different patterns of HIV-specific CTL response can be observed after primary infection. The evolution of infection in these different HIV-seropositive subjects will be particularly interesting to analyse.
Lamhamedi-Cherradi
S
S
Laboratory of Immunology of Cellular and Molecular Interactions, Cochin Hospital, Paris, France.
Culmann-Penciolelli
B
B
Guy
B
B
Ly
T D
TD
Goujard
C
C
Guillet
J G
JG
Gomard
E
E
D000328:Adult; D000368:Aged; D016516:CD4-CD8 Ratio; D002460:Cell Line; D002472:Cell Transformation, Viral; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005809:Genes, Regulator; D005814:Genes, Viral; D006679:HIV Seropositivity / Q000276:immunology*; D015497:HIV-1 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008875:Middle Aged; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013997:Time Factors; D014616:Vaccinia virus / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics
D014764:Viral Proteins; D015678:Viral Structural Proteins