hBD-1: a novel beta-defensin from human plasma.
We report the isolation and characterization of a novel peptide with significant sequence homology to beta-defensins from human blood filtrate. The human beta-defensin-1 (hBD-1) is a short basic peptide of 36 amino acid residues. It contains six cysteines forming three intramolecular disulfide bonds. The molecular mass of hBD-1 is 3928.6 Da. Cloning of the specific cDNA confirmed the amino acid sequence of the native peptide. hBD-1 shares the nine conserved amino acids characteristic for beta-defensins from respiratory epithelial cells and neutrophils of cattle and chicken leukocytes. hBD-1 is present in nanomolar concentration in human plasma.
Bensch
K W
KW
Niedersächsisches Institut für Peptid-Forschung, Hannover, Germany.
Raida
M
M
Mägert
H J
HJ
Schulz-Knappe
P
P
Forssmann
W G
WG
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D001769:Blood; D001798:Blood Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D002417:Cattle; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D023082:Defensins; D005260:Female; D006440:Hemofiltration; D006801:Humans; D007668:Kidney / Q000737:chemistry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014621:Vagina / Q000737:chemistry; D023083:beta-Defensins
D000596:Amino Acids; D001798:Blood Proteins; C406407:DEFB1 protein, human; D018076:DNA, Complementary; D023082:Defensins; D023083:beta-Defensins