Plasma 8-epi-PGF2 alpha levels are elevated in individuals with non-insulin dependent diabetes mellitus.
This study reports plasma levels of a specific nonenzymatic peroxidation product of arachidonic acid, esterified 8-epi-PGF2 alpha, from healthy- and NIDDM individuals as an index of oxidative stress in vivo. Plasma 8-epi-PGF2 alpha was isolated by solid-phase extraction on a C18 followed by an NH2 cartridge and analyzed by GC-MS/NICI as PFB-ester/TMS-ether derivative. We found that the average concentration of esterified 8-epi-PGF2 alpha among NIDDM subjects (0.93 +/- 0.07 nM, n = 39) was higher (P < 0.0001, Mann-Whitney test) than in healthy individuals (0.28 +/- 0.04 nM, n = 15). These data indicate that NIDDM is associated with increased plasma lipid peroxidation.
Gopaul
N K
NK
William Harvey Research Institute, St. Bartholomew's Hospital Medical College, London, UK.
Anggård
E E
EE
Mallet
A I
AI
Betteridge
D J
DJ
Wolff
S P
SP
Nourooz-Zadeh
J
J
D000328:Adult; D001241:Aspirin / Q000494:pharmacology; D002849:Chromatography, Gas / Q000379:methods; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000097:blood; D004951:Esterification; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D013058:Mass Spectrometry / Q000379:methods; D008956:Models, Chemical; D018384:Oxidative Stress
C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D001241:Aspirin; D007213:Indomethacin