title: Identification of two highly homologous presynaptic proteins distinctly localized at the dendritic and somatic synapses.
abstract: Through screening of a murine brain cDNA library, we have isolated two brain specific cDNAs encoding highly homologous proteins, named 921-L and 921-S, comprised of 134 amino acids with 80% identity. Immunohistological study with the mAbs raised against the bacterially expressed 921 proteins showed that 921-L protein is distributed at the dendritic region and 921-S at the neuronal somatic surface. Immuno-electron microscopic study revealed that both 921 proteins are localized at the presynaptic terminal, indicating that the 921 proteins are differentially expressed at the dendritic and somatic presynapses.
authors.affiliation: Department of Immunology, Kochi Medical School, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Z
authors.initials: Z
authors.lastname: Matsuda
authors.forename: M
authors.initials: M
authors.lastname: Ogawa
authors.forename: K
authors.initials: K
authors.lastname: Yagyu
authors.forename: T
authors.initials: T
authors.lastname: Taniguchi
authors.forename: T
authors.initials: T
authors.lastname: Miyata
authors.forename: H
authors.initials: H
authors.lastname: Kaba
authors.forename: T
authors.initials: T
authors.lastname: Higuchi
authors.forename: F
authors.initials: F
authors.lastname: Okutani
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000166:cytology / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D003712:Dendrites / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013569:Synapses / Q000378:metabolism*
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; C095992:complexin I; C095993:complexin II