Immunohistochemical localization of thromboxane receptor and thromboxane synthase in rat testis.
The cellular localization of thromboxane A2 receptor (TXR) and thromboxane synthase (TXS) in rat testes was examined with an antibody against the carboxyl-terminal tail of rat TXR and anti-porcine lung TXS antibody. By light microscopy immunoreactivity for TXR was shown to be present in spermatids, whereas spermatogonia, spermatocytes, and spermatozoa lacked the immunoreactivity. Immunoelectron microscopic analysis revealed that immunostainable TXR was present in acrosomes of spermatids. In contrast, immunoreactivity for TXS was present in all stages of spermatogenic cells; spermatogonia, spermatocytes, spermatids, and spermatozoa. TX system may possibly contribute to the formation of acrosomes or have some unrecognized functions in an autocrine/paracrine fashion.
Takahashi
N
N
Department of Medicine, Tohoku University School of Medicine, Sendai, Japan.
Takeuchi
K
K
Abe
T
T
Murakami
K
K
Yamaguchi
M
M
Abe
K
K
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017482:Receptors, Thromboxane / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D013087:Spermatids / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D013094:Spermatozoa / Q000737:chemistry / Q000166:cytology / Q000648:ultrastructure; D013737:Testis / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D013930:Thromboxane-A Synthase / Q000032:analysis* / Q000737:chemistry / Q000276:immunology
D000906:Antibodies; D009841:Oligonucleotides; D017482:Receptors, Thromboxane; D013930:Thromboxane-A Synthase