Two independent pathways of expression lead to self-assembly of the rabbit hemorrhagic disease virus capsid protein.
The rabbit hemorrhagic disease virus capsid protein was expressed in insect cells either as an individual protein species, from a mRNA analogous to the viral subgenomic RNA, or as part of a polyprotein that included the viral 3C-like protease and the RNA polymerase. Both pathways of expression led to the assembly of viruslike particles morphologically and antigenically similar to purified virus.
Sibilia
M
M
Istituto Zooprofilattico Sperimentale della Lombardia e dell' Emilia, Brescia, Italy.
Boniotti
M B
MB
Angoscini
P
P
Capucci
L
L
Rossi
C
C
D000818:Animals; D016367:Baculoviridae; D001483:Base Sequence; D002213:Capsid / Q000032:analysis / Q000096:biosynthesis*; D002460:Cell Line; D017931:DNA Primers; D015342:DNA Probes; D015870:Gene Expression; D005822:Genetic Vectors; D017929:Hemorrhagic Disease Virus, Rabbit / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D007313:Insecta; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis
D017931:DNA Primers; D015342:DNA Probes; D011994:Recombinant Proteins