Replication of human herpesvirus type 6 (strain AJ) in JJHan cells grown in protein-free medium.
The human T-cell line JJHan, commonly used as a substrate for human herpesvirus type 6 (HHV-6) replication, was established in a protein-free 1:1 mixture of Ham's F12 and Iscove's modified Dulbecco's medium. After 250 passages (4 years) in protein-free medium, the cells, designated JJHan-PF, were cocultured with JJHan cells chronically infected with strain AJ of HHV-6 conventionally grown in serum-supplemented medium. The production of viral antigen was monitored by indirect immunofluorescent antibody staining. The infectivity of the virus in JJHan-PF cells was similar to that in JJHan cultures as shown by HHV-6 antigen detection using a convalescent phase serum. The results show that JJHan-PF cells provide a sensitive substrate for HHV-6 replication without the interference of serum or other proteins usually added to the culture medium.
Cinatl
J
J
Centre of Hygiene, J.W. Goethe University, Frankfurt a. M. Germany.
Cinatl
J
J
Weber
B
B
Rabenau
H
H
Gümbel
H O
HO
Kornhuber
B
B
Doerr
H W
HW
D002460:Cell Line / Q000821:virology*; D003470:Culture Media; D015654:Herpesvirus 6, Human / Q000502:physiology*; D006801:Humans; D013601:T-Lymphocytes / Q000821:virology; D014779:Virus Replication
D003470:Culture Media