Can spin trapping compounds like PBN protect against self-inflicted damage in polymorphonuclear leukocytes?
Polymorphonuclear leukocytes (PMNs) have been suggested to be damaged by superoxide radical generated on their own. The protective capacity of a spin trapping compound, phenyl-N-tert-butyl nitrone (PBN) was evaluated for this damage which occurs after the induction of superoxide generation. The life span of PMNs after superoxide generation was measured in the presence of PBN using the cell counting method, and effects of PBN on the amount of superoxide generated were quantitated using both cytochrome c reduction and spin trapping with DMPO. Results indicated significant extension of life span when PBN was present, and the extension was dose dependent. However, the magnitude of life span extension was not as large as expected from the decrease of superoxide generation. Possible mechanisms for the protection of PMNs by PBN are discussed.
Seawright
L
L
National Biomedical Center for Spin Trapping and Free Radicals, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA.
Tanigawa
M
M
Tanigawa
T
T
Kotake
Y
Y
Janzen
E G
EG
D016923:Cell Death / Q000187:drug effects; D003497:Cyclic N-Oxides; D003574:Cytochrome c Group / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005069:Evaluation Studies as Topic; D006801:Humans; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D009589:Nitrogen Oxides / Q000494:pharmacology*; D010084:Oxidation-Reduction; D013113:Spin Labels; D013481:Superoxides / Q000378:metabolism*; D015996:Survival Rate
D003497:Cyclic N-Oxides; D003574:Cytochrome c Group; D009589:Nitrogen Oxides; D013113:Spin Labels; D013481:Superoxides; C029217:phenyl-N-tert-butylnitrone