Monoclonal antibody mediated capsular reactions (Quellung) in Cryptococcus neoformans.
Capsular reactions ('Quellung') visible by light microscopy were observed when capsule-binding monoclonal antibody (mAb) was added to Cryptococcus neoformans yeast cells. Capsular reactions were observed with capsule-binding IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA mAbs. Scanning electron microscopy revealed that mAb binding produced a structural change in the fibrillar network of the capsule. The occurrence of capsular reactions with mAbs indicate that this phenomenon can be produced by the binding of antibody to a single epitope.
Mukherjee
J
J
Department of Medicine of the Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Cleare
W
W
Casadevall
A
A
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003455:Cryptococcus neoformans / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D011134:Polysaccharides / Q000276:immunology / Q000648:ultrastructure
D000908:Antibodies, Fungal; D000911:Antibodies, Monoclonal; D011134:Polysaccharides; C027478:glucuronoxylomannan