High frequency of human papillomavirus detection in urinary bladder cancer.
We investigated the presence of human papillomaviruses (HPVs) types 16 and 18 DNA in formalin-fixed, paraffin-embedded tissues from the urinary bladder (46 transitional carcinomas and 10 non-neoplastic normal urinary samples) to find a possible role for HPV types in urinary tract cancerogenesis. The analysis was performed using polymerase chain reaction followed by filter hybridization with oligonucleotide-specific probes. The HPV16 and/or HPV18 genomes were detected in 23 of 46 (50%) bladder carcinomas and in none of 10 (0%) non-neoplastic urinary samples. These results suggest that HPV16 and 18 may carry a risk for the development of malignancy in the urinary tract as it occurs in the anogenital regions.
Aglianò
A M
AM
Department of Experimental Medicine, University of Rome La Sapienza, Italy.
Gradilone
A
A
Gazzaniga
P
P
Napolitano
M
M
Vercillo
R
R
Albonici
L
L
Naso
G
G
Manzari
V
V
Frati
L
L
Vecchione
A
A
D002295:Carcinoma, Transitional Cell / Q000821:virology*; D016022:Case-Control Studies; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D016036:Seroepidemiologic Studies; D014412:Tumor Virus Infections / Q000175:diagnosis* / Q000453:epidemiology; D001743:Urinary Bladder / Q000821:virology*; D001749:Urinary Bladder Neoplasms / Q000821:virology*
D015344:DNA Probes, HPV; D004279:DNA, Viral