title: Fungal metabolites. XIX. Structural elucidation of channel-forming peptides, trichorovins-I-XIV, from the fungus Trichoderma viride.
abstract: Trichorovins (TV)-I-XIV are new antibiotic peptides obtained from conidia of the fungus Trichoderma viride. The peptide mixture of TVs was repeatedly fractionated by preparative HPLC until individual TVs showed a single peak on their analytical HPLC chromatograms. Nevertheless, FAB-MS or NMR indicated that each of TVs-I-XIV was composed of at least two components. We attempted to elucidate their structures within the fractions by electrospray ionization (ESI)-MS, FAB-MS, FAB-MS/MS and NMR. TVs generally have molecular weights of approximately 1100-1200 Da, and are characterized by an acetylated N-terminus, the presence of an aminoalcohol, e.g. leucinol, isoleucinol or valinol, at the C-terminus, and eleven residues including three alpha-aminoisobutyric acids in the molecule. Thus, it was determined that TVs belong to the class of peptaibols.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Wada
authors.forename: A
authors.initials: A
authors.lastname: Iida
authors.forename: N
authors.initials: N
authors.lastname: Akimoto
authors.forename: M
authors.initials: M
authors.lastname: Kanai
authors.forename: N
authors.initials: N
authors.lastname: Toyama
authors.forename: T
authors.initials: T
authors.lastname: Fujita
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D000605:Amino Alcohols / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010455:Peptides; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014242:Trichoderma / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D000605:Amino Alcohols; D000900:Anti-Bacterial Agents; D010455:Peptides