Antiproliferative effects of four different cytokines on renal carcinoma cell lines.
The effect of alpha-Interferon (alpha-IFN), gamma-interferon (gamma-IFN), tumor necrosis factor alpha (TNF-alpha) and interleukin-2 (Il-2) on growth of two renal cancer cell lines (DNT-11, HTB-44) was studied. Cell proliferation was measured by a tetrazolium based microculture assay (MTT) after incubation of tumor cells with different concentrations of these 4 cytokines over 72 hours. TNF-alpha showed no antiproliferative effect when cell line DNT-11 was tested, even at high concentrations of 1.000 ng/ml. Proliferation of the other cell line (HTB-44) was only slightly reduced (15%) at 100 ng/ml; a tenfold increase of TNF concentration could not improve these results. The antiproliferative effect of alpha-IFN was in the range of 22-26%. When gamma-IFN was used, both cell lines showed reduction of proliferation between 26% and 37%, respectively. Kinetic studies revealed that prolongation of incubation time for more than 72 hours was not able to improve these results. Il-2 did not influence cell proliferation of either cell line tested, even at high concentrations, as expected.
Buszello
H
H
Department of Urology, Rheinische Friedrich-Wilhelms-Universität, Bonn, Germany.
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002292:Carcinoma, Renal Cell; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D016207:Cytokines / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology; D007371:Interferon-gamma / Q000494:pharmacology; D007376:Interleukin-2 / Q000494:pharmacology; D007680:Kidney Neoplasms; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000970:Antineoplastic Agents; D016207:Cytokines; D016898:Interferon-alpha; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma