title: Antibody to folic acid: increased specificity and sensitivity in ELISA by using epsilon-aminocaproic acid modified BSA as the carrier protein.
abstract: For raising high titre and specific antibody to haptens or drugs, epsilon-aminocaproic acid modified bovine serum albumin (epsilon-ACA-BSA) was prepared for use as a carrier protein. Folic acid (FA) was coupled to epsilon-ACA-BSA, Imj.BSA and BSA for raising antibodies in rabbits. Enhancement of FA immunogenicity with FA-ACA-BSA was observed. Apart from determination of titre by indirect ELISA, dose-response behaviour and specificity of these antisera were also compared. FA-ACA-BSA antibody showed high sensitivity and specificity. Using this antibody, an ELISA method for the determination of FA was developed. The study provides a simple approach to raise highly specific and high titre antibody against small molecules.
authors.affiliation: Indian Statistical Institute, Calcutta, India.
authors.forename: J
authors.initials: J
authors.lastname: Das Sarma
authors.forename: C
authors.initials: C
authors.lastname: Duttagupta
authors.forename: E
authors.initials: E
authors.lastname: Ali
authors.forename: T K
authors.initials: TK
authors.lastname: Dhar
mesh_terms: D015119:Aminocaproic Acid / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D005492:Folic Acid / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000276:immunology*
chemical_list: D000906:Antibodies; D012710:Serum Albumin, Bovine; D005492:Folic Acid; D015119:Aminocaproic Acid