Physical mapping and characterization of a catabolic plasmid from the deep-subsurface bacterium Sphingomonas sp. strain F199.
A supercoiled 180-kb plasmid, pNL1, has been isolated from the deep-subsurface, chemoheterotrophic Sphingomonas sp. strain F199, and a physical map was generated. Analysis of a pNL1-derived cosmid library indicated that catechol 2,3-dioxygenase activity was linked to two distinct regions of the plasmid. Thus, the genes for aromatic catabolism in this Sphingomonas strain are, at least in part, plasmid encoded.
Stillwell
L C
LC
Pacific Northwest Laboratory, Richland, Washington 99352, USA.
Thurston
S J
SJ
Schneider
R P
RP
Romine
M F
MF
Fredrickson
J K
JK
Saffer
J D
JD
D050557:Catechol 2,3-Dioxygenase; D049308:Dioxygenases; D015964:Gene Expression Regulation, Bacterial; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology / Q000235:genetics*; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping
D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase