Distribution of beta 3-adrenoceptor mRNA in human tissues.
The beta 3-adrenoceptor is a G protein-coupled receptor which mediates metabolic functions of the endogenous catecholamines epinephrine and norepinephrine. Questions exist regarding distribution of the beta 3-adrenoceptor in human tissue. In order to examine the distribution of beta 3-adrenoceptor mRNA in human tissues, we used sensitive and specific RNase protection assays without previous PCR amplification in an extensive list of human tissues. We confirm the presence of beta 3-adrenoceptor mRNA in human white fat from several locations, gall bladder, and small intestine, as well as extend the distribution of beta 3-adrenoceptor mRNA to previously uncharacterized human tissues such as stomach and prostate. The presence of beta 3-adrenoceptor mRNA in human white adipose tissue has important implications regarding possible use of beta 3-adrenoceptor selective agonists as anti-obesity agents, and the demonstration of beta 3-adrenoceptor mRNA in a number of gastrointestinal tissues and prostate raises the question of the role of the beta 3-adrenoceptor in motility and secretory processes.
Berkowitz
D E
DE
Department of Anesthesiology, Duke University Medical Center, Durham, NC 27710, USA.
Nardone
N A
NA
Smiley
R M
RM
Price
D T
DT
Kreutter
D K
DK
Fremeau
R T
RT
Schwinn
D A
DA
D000818:Animals; D001345:Autoradiography; D016466:CHO Cells; D002460:Cell Line; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism; D006224:Cricetinae; D018076:DNA, Complementary; D005704:Gallbladder / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*; D014018:Tissue Distribution
D018076:DNA, Complementary; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta