Kinetic behaviour of pancreatic lipase in five species using emulsions and monomolecular films of synthetic glycerides.
In the absence of colipase and bile salts, using tributyrin emulsions or monomolecular films of dicaprin at low surface pressure, we observed that no significant lipase activity can be measured with Human Pancreatic Lipase (HuPL), Horse Pancreatic Lipase (HoPL) or Dog Pancreatic Lipase (DPL). Only Porcine Pancreatic Lipase (PPL) and recombinant Guinea Pig Pancreatic Lipase Related Protein of type 2 (r-GPL) hydrolyse pure tributyrin in the absence of any additive, as well as dicaprin films at low surface pressures. The former lipases may lack enzyme activity because of irreversible interfacial denaturation due to the high energy existing at the tributyrin/water interface and at the dicaprin film surface at low surface pressures. The enzyme denaturation cannot be reflected in the number of disulfide bridges, since all the pancreatic lipases tested here contain six disulfide bridges, but behaved very differently at interfaces. We propose to use the surface pressure threshold, as determined using the monomolecular technique, as a criterion for classifying lipases in terms of their sensitivity to interfacial denaturation.
Gargouri
Y
Y
Laboratoire de Biochimie, Ecole Nationale d'Ingénieurs de Sfax, Tunisia.
Bensalah
A
A
Douchet
I
I
Verger
R
R
D000818:Animals; D003089:Colipases / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism*; D004285:Dogs; D004655:Emulsions; D006168:Guinea Pigs; D006736:Horses; D006801:Humans; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D011994:Recombinant Proteins / Q000378:metabolism; D013045:Species Specificity; D013270:Stomach / Q000201:enzymology; D013499:Surface Properties; D013500:Surface Tension; D013552:Swine; D013657:Taurodeoxycholic Acid / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism*
D003089:Colipases; D004075:Diglycerides; D004655:Emulsions; D011994:Recombinant Proteins; D014280:Triglycerides; C044297:1,2-didecanoylglycerol; D013657:Taurodeoxycholic Acid; D008049:Lipase; C075988:pancreatic lipase related protein 2; C005830:tributyrin