Effects of taurine on liver preservation in UW solution with consecutive ischemic rewarming in the isolated perfused rat liver.
Taurine (2-aminoethane sulfonic acid) is a physiologic amino acid involved in cellular osmoregulation in various species including man. This study was intended to compare the respective effects of cold storage and consecutive ischemic rewarming of the liver postischemic hepatic flow and hepatocellular outcome upon reperfusion with or without the addition of taurine to the preservation medium. Livers from male Wistar rats were rinsed free of blood via the portal vein and stored ischemically at 4 degrees C in UW solution. Livers from group 1 were then rinsed again with 10 ml Ringer's solution and reperfused with Krebs-Henseleit buffer at a constant pressure of 10 mmHg for 45 min at 37 degrees C in a nonrecirculating manner. Livers from groups 2 and 3 were subjected to 30 min of warm ischemia subsequent to cold storage and prior to reperfusion with 10 mM taurine added to the UW solution in group 3. While there were only very few signs of hepatic injury in group 1, the additional period of warm ischemia (group 2) led to a significant reduction in early perfusate flow and enhanced enzyme leakage from the livers during postischemic rinse and reperfusion. Livers in group 3 exhibited an amelioration in hepatic circulation and significantly reduced enzyme release as compared to group 2. The results clearly demonstrate a remarkable impact of postischemic rewarming on graft viability. Furthermore, the addition of taurine to the preservation medium was shown to improve hepatic circulation and enhance viability of the liver upon reperfusion.
Minor
T
T
Institut für Experimentelle Medizin, Universität Köln, Germany.
Yamaguchi
T
T
Isselhard
W
W
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D005978:Glutathione; D007328:Insulin; D007511:Ischemia / Q000503:physiopathology; D008099:Liver / Q000187:drug effects* / Q000503:physiopathology; D008297:Male; D019188:Organ Preservation Solutions; D010477:Perfusion; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000494:pharmacology*; D013696:Temperature; D014021:Tissue Preservation
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D013654:Taurine; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose