Effects of exposure to environmental tobacco smoke on a human tracheobronchial epithelial cell line.
BEAS-2B cells, a human bronchial epithelial line immortalized by viral transformation, were exposed to sidestream tobacco smoke (STS) as a surrogate for environmental tobacco smoke (ETS) under biphasic culture conditions where the apical portion of the cells was in direct contact with the gas phase. Dose-dependent cytotoxicity was observed. In addition, induction of an as yet uncharacterized protein of molecular weight 45,000 was associated with exposure to STS. This protein might be part of a protective response of exposed cells, which do not show a classical heat shock response when exposed to STS. We conclude that STS and ETS can be directly cytotoxic to human airway epithelial cells in biphasic culture at concentrations not unreasonable for smoky indoor atmospheres. The model system described in this paper should be useful for studying the detailed mechanisms of cytotoxicity of, and protection from, ETS exposure in the human cells most directly exposed to ETS in vivo.
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Department of Internal Medicine, School of Medicine, University of California, Davis 95616, USA.
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D001980:Bronchi / Q000166:cytology / Q000473:pathology*; D002452:Cell Count / Q000187:drug effects; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells; D004848:Epithelium / Q000473:pathology; D006358:Hot Temperature / Q000009:adverse effects; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008970:Molecular Weight; D010078:Oxazines; D010126:Ozone / Q000009:adverse effects; D010455:Peptides / Q000378:metabolism; D014176:Protein Biosynthesis; D011500:Protein Synthesis Inhibitors / Q000009:adverse effects; D013936:Thymidine / Q000378:metabolism; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D014132:Trachea / Q000166:cytology / Q000473:pathology*; D014966:Xanthenes
D004396:Coloring Agents; D010078:Oxazines; D010455:Peptides; D011500:Protein Synthesis Inhibitors; D014028:Tobacco Smoke Pollution; D014966:Xanthenes; C005843:resazurin; D010126:Ozone; D013936:Thymidine