Incorporation of bovine adrenal 3 beta-hydroxy-5-ene steroid dehydrogenase/delta 5-delta 4 isomerase into phospholipid vesicles.
3 beta-Hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase (3 beta-HSD/I) and cytochrome P-450C21 were co-purified from bovine adrenocortical microsomes by an improved method. The 3 beta-HSD/I was successfully incorporated into liposomal membranes in which the enzyme activity was greatly stabilized. Enzymatic activities and kinetic parameters of the 3 beta-HSD/I proteoliposomes were almost the same as those of the solubilized form.
Yamazaki
T
T
Faculty of Integrated Arts and Sciences, Hiroshima University, Japan.
Kominami
S
S
Nishida
N
N
Takemori
S
S
D006907:17-alpha-Hydroxypregnenolone / Q000378:metabolism; D000302:Adrenal Cortex / Q000201:enzymology*; D000818:Animals; D002417:Cattle; D002499:Centrifugation, Density Gradient / Q000379:methods; D019826:Cholic Acid; D002793:Cholic Acids / Q000494:pharmacology; D002850:Chromatography, Gel; D003577:Cytochrome P-450 Enzyme System / Q000302:isolation & purification / Q000378:metabolism; D003687:Dehydroepiandrosterone / Q000378:metabolism; D004795:Enzyme Stability; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology; D009097:Multienzyme Complexes / Q000302:isolation & purification / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011284:Pregnenolone / Q000378:metabolism; D011375:Progesterone Reductase / Q000302:isolation & purification / Q000378:metabolism*; D013053:Spectrophotometry; D013251:Steroid Isomerases / Q000302:isolation & purification / Q000378:metabolism*
C021425:3 beta-hydroxysteroid oxidoreductase-delta(5) 3-ketosteroid isomerase; D002793:Cholic Acids; D008081:Liposomes; D009097:Multienzyme Complexes; D010743:Phospholipids; D009243:NAD; D006907:17-alpha-Hydroxypregnenolone; D003687:Dehydroepiandrosterone; D011284:Pregnenolone; D003577:Cytochrome P-450 Enzyme System; D011375:Progesterone Reductase; D013251:Steroid Isomerases; D019826:Cholic Acid