title: Cytokinetic studies of oral cancer cells using bromodeoxyuridine labelling in relation to factors influencing prognosis.
abstract: Cytokinetics of 27 untreated oral squamous cell carcinomas were evaluated by in vitro bromodeoxyuridine (BrdU) labelling. A mononuclear cell suspension was prepared for the labelling using collagenase and DNase treatment. The labelled cells were visualised by immunofluorescence staining with an anti-BrdU monoclonal antibody. The labelling index (LI%) was calculated by determining the percentage of BrdU labelled cells. The LIs of the carcinomas ranged from 3.8 to 19.2%. The LIs of poorly-differentiated (HG3) tumours were 2-3-fold higher compared with those of well-differentiated (HG1) and moderately-differentiated (HG2) carcinomas. Results also showed statistically significant (P < 0.0005) increases in LIs from nuclear grade 1 (NG1) to nuclear grade 3 (NG3) carcinomas. Higher LIs were observed in stage III/IV (10.82 +/- 4.62; 10.36 +/- 4.90) than those in stage I (6.87 +/- 2.09) and II (7.14 +/- 1.87) carcinomas. A significant (P < 0.0005) difference in LI values was found between the patients with positive and negative lymph nodes. Good correlation (r = 0.77) was exhibited between the LI values and mitotic counts (MC) of the specimens. These results on oral cancer cell proliferation seem to have prognostic implications.
authors.affiliation: Department of Tumour Virology, Chittaranjan National Cancer Institute, Calcutta, India.
authors.forename: D
authors.initials: D
authors.lastname: Mukhopadhyay
authors.forename: R
authors.initials: R
authors.lastname: Chatterjee
authors.forename: R N
authors.initials: RN
authors.lastname: Chakraborty
mesh_terms: D000328:Adult; D000368:Aged; D001973:Bromodeoxyuridine; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D002453:Cell Cycle; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D008940:Mitotic Index; D009062:Mouth Neoplasms / Q000473:pathology*; D011379:Prognosis
chemical_list: D001973:Bromodeoxyuridine