Nitric oxide production and mycobacterial growth inhibition by murine alveolar macrophages: the sequence of rIFN-gamma stimulation and Mycobacterium bovis BCG infection determines macrophage activation.
Prestimulation with recombinant-interferon-gamma (rIFN-gamma) followed by Mycobacterium bovis BCG infection induced high nitric oxide production and potent mycobacterial growth inhibition in murine alveolar macrophages. Reversal of the sequence of treatments caused opposite effects, suggesting that a sequential 2-step process comprising first rIFN-gamma stimulation and second mycobacterial infection is operative in the activation of alveolar macrophages.
Hanano
R
R
Department of Immunology, University of Ulm, Germany.
Kaufmann
S H
SH
D000818:Animals; D015415:Biomarkers; D007371:Interferon-gamma / Q000494:pharmacology; D008262:Macrophage Activation / Q000187:drug effects*; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009163:Mycobacterium bovis / Q000254:growth & development; D009252:NADPH Dehydrogenase / Q000032:analysis; D009569:Nitric Oxide / Q000096:biosynthesis*; D011994:Recombinant Proteins; D013047:Specific Pathogen-Free Organisms; D014376:Tuberculosis / Q000276:immunology / Q000382:microbiology
D015415:Biomarkers; D011994:Recombinant Proteins; D009569:Nitric Oxide; D007371:Interferon-gamma; D009252:NADPH Dehydrogenase