[Increased cell adhesiveness and decreased tumorigenicity induced in human colon carcinoma cells by transfection with E-cadherin cDNA].
Metastasis of colon carcinomas is assumed to be caused by multiple steps, which include a loss of cell adhesion that results in the release of carcinoma cells from the original tumor tissue. A human colon carcinoma cell line was established from a poorly differentiated metastatic adenocarcinoma without cell-cell adhesion and without expression of E-cadherin mRNA. To this cell line, mouse E-cadherin cDNA in a expression vector was co-transfected with a neomycin-resistant gene. The transfected cells, which expressed exogenous E-cadherin gene, showed a compact shape with strong cell-cell adhesion and with increased cell-substratum adhesion. These cells showed a significantly low anchorage independency and decreased invasiveness compared to the parental carcinoma cells. Their growth rate was decreased both in vitro and in the subcutis of nude mice.
Miyaki
M
M
Department of Biochemistry, Tokyo Metropolitan Institute of Medical Science.
D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D015820:Cadherins / Q000235:genetics*; D002448:Cell Adhesion / Q000502:physiology*; D003110:Colonic Neoplasms / Q000473:pathology*; D018076:DNA, Complementary; D006801:Humans; D051379:Mice; D014162:Transfection; D014407:Tumor Cells, Cultured
D015820:Cadherins; D018076:DNA, Complementary