Interaction of complement and specific antibodies with the external glycoprotein 120 of HIV-1.
Previously we have investigated the interaction of human complement as well as one polyclonal and three human monoclonal antibody preparations with the human immunodeficiency virus type-1 (HIV-1) transmembrane recombinant glycoprotein (rgp41). A strong competition was found between the antibodies and deposited complement proteins for the same binding sites located within the immunodominant region of rgp41. The aim of the present experiments was to see if the same type of antibody-complement-HIV-1 interactions could be observed with the outer envelope glycoprotein (rgp120) of HIV-1. Three different glycosylated rgp120 preparations, as well as a synthetic peptide corresponding to the V3 loop of the MN strain, were adsorbed to enzyme-linked immunosorbent assay (ELISA) plates and incubated with mixtures of anti-rgp120 antibodies and normal human serum (NHS) as a complement source. Fixed complement proteins and antibodies were detected with specific, peroxidase-labelled antibodies against different complement proteins (C1q, C4b, C3b) and the gamma-chain of antibodies. In the absence of anti-rgp120, high amounts of C3 were deposited to each rgp120 preparation tested (including the V3 peptide) but significant differences in the amounts of bound C1q and C4b were observed. Using sera deficient in different complement proteins, we found that both the classical and the alternative pathways contributed to the C3 binding to rgp120. Addition of specific antibodies did not increase complement activation by rgp120 and only in the case of a monoclonal antibody to the V3-loop could we see complement-dependent inhibition of antibody binding.
Prohászka
Z
Z
National Institute of Haematology, Blood Transfusion and Immunology, Budapest, Hungary.
Hidvégi
T
T
Ujhelyi
E
E
Stoiber
H
H
Dierich
M P
MP
Süsal
C
C
Füst
G
G
D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D015922:Complement C1q / Q000276:immunology; D003176:Complement C3 / Q000276:immunology*; D003179:Complement C3b / Q000276:immunology; D015935:Complement C4b / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D003176:Complement C3; D015699:HIV Envelope Protein gp120; D015922:Complement C1q; D003179:Complement C3b; D015935:Complement C4b