title: Heparin binding by fibronectin module III-13 involves six discontinuous basic residues brought together to form a cationic cradle.
abstract: The thirteenth type III domain of fibronectin binds heparin almost as well as fibronectin itself and contains a so-called heparin-binding consensus sequence, Arg6-Arg7-Ala8-Arg9 (residues 1697-1700 in plasma fibronectin). Barkalow and Schwarzbauer (Barkalow, F.J., and Schwarzbauer, J.E. (1991) J. Biol. Chem. 266, 7812-7818) showed that mutation of Arg6-Arg7 in domain III-13 of recombinant truncated fibronectins abolished their ability to bind heparin-Sepharose. However, synthetic peptides containing this sequence have negligible affinity for heparin (Ingham, K.C., Brew, S.A., Migliorini, M. M., and Busby, T.F. (1993) Biochemistry 32, 12548-12553). We generated a three-dimensional model of fibronectin type III-13 based on the structure of a homologous domain from tenascin. The model places Arg23, Lys25, and Arg54 parallel to and in close proximity to the Arg6-Arg7-Ala8-Arg9 motif, suggesting that these residues may also contribute to the heparin-binding site. Domain III-13 and six single-site mutants containing Ser in place of each of the above-mentioned basic residues were expressed in Escherichia coli. All of the purified mutant domains melted reversibly with a Tm near that of the wild type indicating that they were correctly folded. When fluorescein-labeled heparin was titrated at physiological ionic strength, the wild type domain increased the anisotropy in a hyperbolic fashion with a Kd of 5-7 microM, close to that of the natural domain obtained by proteolysis of fibronectin. The R54S mutant bound 3-fold weaker and the remaining mutants bound at least 10-fold weaker than wild type. The results point out that the Arg6-Arg7-Ala8-Arg9 consensus sequence by itself has little affinity for heparin under physiological conditions, even when presented in the context of a folded domain. Thus, the heparin-binding site in fibronectin is more complex than previously realized. It is formed by a cluster of 6 positively charged residues that are remote in the sequence but brought together on one side of domain III-13 to form a "cationic cradle" into which the anionic heparin molecule could fit.
authors.affiliation: Holland Laboratory, American Red Cross, Rockville, Maryland 20855, USA.
authors.forename: T F
authors.initials: TF
authors.lastname: Busby
authors.forename: W S
authors.initials: WS
authors.lastname: Argraves
authors.forename: S A
authors.initials: SA
authors.lastname: Brew
authors.forename: I
authors.initials: I
authors.lastname: Pechik
authors.forename: G L
authors.initials: GL
authors.lastname: Gilliland
authors.forename: K C
authors.initials: KC
authors.lastname: Ingham
mesh_terms: D000595:Amino Acid Sequence; D000599:Amino Acids, Diamino / Q000378:metabolism; D001665:Binding Sites; D002412:Cations; D004252:DNA Mutational Analysis; D005353:Fibronectins / Q000235:genetics / Q000378:metabolism*; D019793:Fluorescein; D005452:Fluoresceins; D005454:Fluorescence Polarization; D006493:Heparin / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D000599:Amino Acids, Diamino; D002412:Cations; D005353:Fibronectins; D005452:Fluoresceins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D006493:Heparin; D019793:Fluorescein