title: Effect of bisphosphonates and gallium on dentin resorption in vitro.
abstract: Replacement resorption may follow the replantation of an avulsed tooth. Currently there is no effective treatment for replacement resorption. The purpose of this study was to investigate the effect of bisphosphonates and gallium nitrate, which have been shown to reduce bone resorption, on cells which resorb dentin. Osteoclast-like cells were obtained by culturing cells from prenatal chick tibeas. These cells were seeded onto slices of human dentin which had been soaked in either saline (control), or solutions of 10(-5) M 1-hydroxyethylidene-1, 1-bisphosphonic acid (EHBP), 10(-6) M dichloromethylene bisphosphonic acid (Cl2MBP), or 10(-6) M gallium nitrate. Resorption was measured by counting the number of resorptive lacunae produced by the cells. Results indicated that the experimental groups did not differ significantly from each other, but each exhibited significantly reduced resorption compared with saline controls (p < 0.01). These results suggested that the experimental treatment reduced dentinal resorption by the osteoclast-like cells, and that these agents might be useful to prevent or at least postpone replacement resorption in avulsed teeth.
authors.affiliation: U.S. Army Dental Activity, Clinical Investigation, Eisenhower Army Medical Center, Fort Gordon, Medical College of Georgia, School of Dentistry, Augusta, USA.
authors.forename: F R
authors.initials: FR
authors.lastname: Liewehr
authors.forename: D W
authors.initials: DW
authors.lastname: Craft
authors.forename: P D
authors.initials: PD
authors.lastname: Primack
authors.forename: J C
authors.initials: JC
authors.lastname: Kulild
authors.forename: D K
authors.initials: DK
authors.lastname: Turgeon
authors.forename: D E
authors.initials: DE
authors.lastname: Sutherland
authors.forename: G S
authors.initials: GS
authors.lastname: Schuster
authors.forename: D H
authors.initials: DH
authors.lastname: Pashley
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002642:Chick Embryo; D003804:Dentin / Q000187:drug effects* / Q000378:metabolism; D011756:Diphosphates / Q000494:pharmacology* / Q000627:therapeutic use; D005708:Gallium / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D066298:In Vitro Techniques; D008964:Molar, Third; D010010:Osteoclasts / Q000187:drug effects; D012391:Root Resorption / Q000517:prevention & control*; D014084:Tooth Avulsion; D014090:Tooth Replantation
chemical_list: D011756:Diphosphates; D005708:Gallium