Homogenous IgA monomers, dimers, trimers and tetramers from the same IgA myeloma serum.
Starting from two IgA1 myeloma sera, the isolation of monoclonal monomeric, dimeric, trimeric and tetrameric IgA in a high state of purity and size homogeneity for each serum is described. The method combined repetitive gel filtrations on Ultrogel AcA22 with affinity chromatography on Jacalin-Sepharose. These various forms of pure polymeric IgA obtained from the same monoclonal IgA should allow a precise comparison of their respective structure and reactivity with different IgA-binding proteins, such as IgA Fc-receptors, the polymeric Ig receptor, and lectins.
Vaerman
J P
JP
Unit of Experimental Medicine, Catholic University of Louvain, Belgium.
Langendries
A
A
Vander Maelen
C
C
D000911:Antibodies, Monoclonal / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification*; D007144:Immunoglobulin J-Chains / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D007145:Immunoglobulin kappa-Chains / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D007146:Immunoglobulin lambda-Chains / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D037102:Lectins; D009101:Multiple Myeloma / Q000097:blood*; D009194:Myeloma Proteins / Q000737:chemistry* / Q000302:isolation & purification; D037121:Plant Lectins; D011487:Protein Conformation; D012685:Sepharose
D000911:Antibodies, Monoclonal; D007070:Immunoglobulin A; D007144:Immunoglobulin J-Chains; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D037102:Lectins; D009194:Myeloma Proteins; D037121:Plant Lectins; C043696:jacalin; C034639:polymeric IgA; D012685:Sepharose