Concanamycin B inhibits the expression of newly-synthesized MHC class II molecules on the cell surface.
We screened natural chemical compounds which inhibit the expression of newly-synthesized MHC class II molecules on the cell surface. IFN-gamma stimulates Colo 205 adenocarcinoma cells to induce the expression of MHC class I, MHC class II, and ICAM-1 molecules on the cell surface. We show here that concanamycin B inhibits the expression of MHC class II molecules on Colo 205 cells, whereas it does not affect the expression of MHC class I and ICAM-1 molecules. Concanamycin B also abrogates the enhancement of the MHC class II expression by IL-4 on mouse lymphocytes. Furthermore, concanamycin B suppresses the antigen presentation by MHC class II molecules.
Ito
K
K
Central Research Laboratories of Ajinomoto Co. Inc., Kanagawa, Japan.
Kobayashi
T
T
Moriyama
Y
Y
Toshima
K
K
Tatsuta
K
K
Kakiuchi
T
T
Futai
M
M
Ploegh
H L
HL
Miwa
K
K
D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000276:immunology / Q000494:pharmacology*; D000938:Antigen-Presenting Cells / Q000187:drug effects / Q000276:immunology; D001483:Base Sequence; D002462:Cell Membrane / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D005802:Genes, MHC Class II / Q000187:drug effects; D000949:Histocompatibility Antigens Class II / Q000187:drug effects* / Q000276:immunology; D018942:Macrolides; D051379:Mice; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000900:Anti-Bacterial Agents; D000949:Histocompatibility Antigens Class II; D018942:Macrolides; C036979:concanamycin B; D006180:Proton-Translocating ATPases