Rapid and transient phosphorylation of nuclear matrix proteins upon interferon-alpha treatment in Daudi lymphoma cells.
Evidence for a rapid and transient hyperphosphorylation of a 45 kD protein in isolated nuclei from interferon-alpha-treated Daudi lymphoma cells is presented. Extraction of nuclear matrices from these nuclei has provided further evidence for the association of such a protein in the nuclear matrix structure. Because phosphorylation assays performed directly on nuclear matrices from interferon-treated cells have revealed rapid and transient increase of gamma 32P-ATP incorporation into this 45 kD band, an early involvement of the nuclear matrix in the response of the nucleus to the interferon antiproliferative message is suggested.
Cataldi
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Istituto di Morfologia Umana Normale, Universitá G. D'Annunzio, Chieti, Italy.
Antonucci
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Robuffo
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I
Di Baldassarre
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A
Miscia
S
S
D034961:Antigens, Nuclear; D002467:Cell Nucleus / Q000378:metabolism; D006801:Humans; D007370:Interferon Type I / Q000494:pharmacology; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology*; D008223:Lymphoma / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D010766:Phosphorylation; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D034961:Antigens, Nuclear; D007370:Interferon Type I; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D009687:Nuclear Proteins; D011994:Recombinant Proteins