Expression and activity-dependent changes of a novel limbic-serine protease gene in the hippocampus.
A novel murine cDNA which encodes a protein designated neuropsin was cloned. Northern and in situ hybridization analyses demonstrated that neuropsin mRNA is expressed specifically in the limbic system of mouse brain and is localized at highest concentration in pyramidal neurons of the hippocampal CA1-3 subfields. Direct hippocampal stimulation and kindling induced by amygdaloid stimulation caused a significant bilateral change in neuropsin mRNA level in the hippocampal pyramidal neurons. The activity-dependent changes and the specific localization indicate that neuropsin is involved in hippocampal plasticity.
Chen
Z L
ZL
Department of Structural Cellular Biology, Nara Institute of Science and Technology (NAIST), Japan.
Yoshida
S
S
Kato
K
K
Momota
Y
Y
Suzuki
J
J
Tanaka
T
T
Ito
J
J
Nishino
H
H
Aimoto
S
S
Kiyama
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004558:Electric Stimulation; D015870:Gene Expression; D006624:Hippocampus / Q000502:physiology*; D007610:Kallikreins; D007696:Kindling, Neurologic; D008032:Limbic System / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
D018076:DNA, Complementary; D012333:RNA, Messenger; C094799:KLK8 protein, human; D007610:Kallikreins; D012697:Serine Endopeptidases