title: Isolation and chromosomal localization of a human ATP-regulated potassium channel.
abstract: KATP channels are K+ channels whose activity is inhibited by the presence of and enhanced by the absence of cytosolic ATP. This property allows KATP channels to sense cellular intermediary metabolism and directly couple this information to the modulation of membrane excitability. Indeed, recent studies from our laboratory and others have suggested that activation of KATP channels during anoxia is important in the response and adaptation of central neurons to hypoxia. In order to identify KATP channels from human brain, we performed a polymerase chain reaction (PCR) using human cerebral cortex mRNA and primers derived from the ROMK1 sequence, a cDNA clone encoding an ATP-regulated potassium channel, recently isolated from rat kidney. We thus identified a novel 308-bp PCR product, pKCNJ1, whose expression was found to be restricted to a 3.0-kb band in the kidney by probing a human multiple tissue northern blot, pKCNJ1 was then used to isolate genomic clones and, using fluorescence in situ hybridization (FISH) to human metaphase chromosomes, was mapped to chromosome 11q.
authors.affiliation: Department of Pediatrics (Section of Respiratory Medicine), Yale University School of Medicine, New Haven, CT 06520-8064, USA.
authors.forename: S N
authors.initials: SN
authors.lastname: Krishnan
authors.forename: T
authors.initials: T
authors.lastname: Desai
authors.forename: D C
authors.initials: DC
authors.lastname: Ward
authors.forename: G G
authors.initials: GG
authors.lastname: Haddad
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015640:Ion Channel Gating; D007668:Kidney / Q000737:chemistry; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015221:Potassium Channels / Q000187:drug effects / Q000235:genetics*; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D013702:Temporal Lobe / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: C086989:KCNJ1 protein, human; C510692:Kcnj1 protein, rat; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D012333:RNA, Messenger; D000255:Adenosine Triphosphate