Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus.
Mutants of the extreme thermophile Thermus flavus in the pyrimidine biosynthetic pathway (Pyr-) were isolated by resistance to 5-fluoroorotic acid. The pyrE gene, which codes for the orotate phosphoribosyltransferase, was cloned by recombination with one of the isolated Pyr- T. flavus mutant strains. It was subcloned by complementation of an Escherichia coli pyrE mutant strain and was sequenced. The deduced polypeptide sequence extends over 183 amino acids. Several independent Pyr- mutations were mapped within the pyrE locus by recombination with fragments of the cloned gene.
Vonstein
V
V
ThermoGen, Inc., Chicago, Illinois 60612, USA.
Johnson
S P
SP
Yu
H
H
Casadaban
M J
MJ
Pagratis
N C
NC
Weber
J M
JM
Demirjian
D C
DC
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009154:Mutation; D009962:Orotate Phosphoribosyltransferase / Q000096:biosynthesis / Q000235:genetics; D013824:Thermus / Q000201:enzymology / Q000235:genetics*
D004269:DNA, Bacterial; D009962:Orotate Phosphoribosyltransferase