Enantioselective high-performance liquid chromatographic determination of omeprazole in human plasma.
A new stereoselective HPLC assay was developed to isolate omeprazole enantiomers from human plasma using C2 solid-phase extraction cartridges and an analogue was used as internal standard. Recoveries of the (+)-isomer were 83.4 and 89.7% at 100 and 250 ng/ml, respectively. Recoveries of the (-)-isomer were 78.4 and 82.8%, respectively. Recovery of the internal standard averaged 77.2%. Direct chiral separation of the enantiomers is achieved on a Resolvosil BSA-7 chiral column (15 cm x 4 mm I.D.) and a matching guard column. The mobile phase is a variable amount of n-propanol (0.05-1.0%) in 0.05 M ammonium phosphate buffer (pH 7.0) and the flow-rate is 1.5 ml/min. Drug absorbance is monitored at 302 nm. Standard curves are linear from 15 to 250 ng/ml for each enantiomer. The coefficients of variation for intra-day precision at each concentration over the range of the standard curve were between 0.98 and 10.87%. The coefficients of variation for inter-day precision for the analyses of omeprazole enantiomers in plasma (30 and 175 ng/ml) were less than 10% over a four month interval.
Cairns
A M
AM
Department of Drug Metabolism, Merck Research Laboratories, West Point, PA 19486, USA.
Chiou
R H
RH
Rogers
J D
JD
Demetriades
J L
JL
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009853:Omeprazole / Q000097:blood*; D012015:Reference Standards; D015203:Reproducibility of Results; D013237:Stereoisomerism
D009853:Omeprazole