Structural organization of the gene encoding the human iron-sulfur subunit of succinate dehydrogenase.
The iron-sulfur protein (Ip) subunit of succinate dehydrogenase (SDH and complex II) of the respiratory chain is highly conserved in evolution [Gould et al., Proc. Natl. Acad. Sci. USA 86 (1989) 1934-1938]. We have cloned the entire human Ip cDNA, as well as the Ip-encoding gene (SDH-B) from two genomic human libraries. The cDNA contains a coding sequence of 840 nt, flanked by a 5'-UTR of 133 nt and a 3'-UTR of 123 nt. The entire transcript is encoded by eight exons within approx. 40 kb. The seven introns range in size from 0.75 kb to > 11 kb, and they appear to be of the 'late' intron class. Approx. 5 kb of upstream sequence was also cloned, and approx. 2.4 kb of the promoter region were sequenced and analyzed for consensus elements binding potential transcription factors and transcriptional activators.
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Department of Biology, University of California at San Diego, La Jolla 92093-0322, USA.
Ream-Robinson
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Bellew
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Broomfield
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Saghbini
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Scheffler
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D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons / Q000235:genetics; D015698:Genomic Library; D006801:Humans; D007438:Introns / Q000235:genetics; D007506:Iron-Sulfur Proteins / Q000235:genetics*; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D011487:Protein Conformation; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013385:Succinate Dehydrogenase / Q000235:genetics*
D018076:DNA, Complementary; D007506:Iron-Sulfur Proteins; D013385:Succinate Dehydrogenase