Construction and heterologous expression of a synthetic copy of the cutinase cDNA from Fusarium solani pisi.
A copy of the cutinase cDNA from Fusarium solani pisi was constructed starting from synthetic oligonucleotides. For this construction three separate cassettes were made, which were subsequently assembled to form the cutinase gene. Heterologous expression of the synthetic cutinase gene and the subsequent secretion of the recombinant enzyme was achieved in Saccharomyces cerevisiae and Aspergillus awamori.
van Gemeren
I A
IA
Department of Molecular and Cellular Biology, University of Utrecht, The Netherlands.
Musters
W
W
van den Hondel
C A
CA
Verrips
C T
CT
D000595:Amino Acid Sequence; D001230:Aspergillus / Q000235:genetics; D001483:Base Sequence; D001709:Biotechnology; D002265:Carboxylic Ester Hydrolases / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics*; D005670:Fusarium / Q000201:enzymology* / Q000235:genetics*; D005800:Genes, Fungal; D005813:Genes, Synthetic; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics
D018076:DNA, Complementary; D004271:DNA, Fungal; D011994:Recombinant Proteins; D002265:Carboxylic Ester Hydrolases; C019952:cutinase