title: Regulation of human cytomegalovirus US3 gene transcription by a cis-repressive sequence.
abstract: Regulation of immediate-early gene expression in human cytomegalovirus is subject to complex controls. The major immediate-early (mIE) gene is regulated by both positive and negative regulatory signals, including autoregulation mediated by a cis-repressive sequence. A second immediate-early gene, the US3 gene, is transcribed with kinetic similar to those of the mIE gene. I have identified an element present in the US3 gene located from -1 to -13 (relative to the start site of transcription) that mediates a decrease in US3 transcription. The US3 element resembles the cis-repressive element of the mIE gene in sequence, position, and function. The common theme of negative regulation of immediate-early genes shortly after infection suggests that a decrease in the level of immediate-early proteins may be critical for viral replication.
authors.affiliation: Department of Biological Sciences, College of Osteopathic Medicine, Ohio University, Athens 45701, USA.
authors.forename: B J
authors.initials: BJ
authors.lastname: Biegalke
mesh_terms: D001483:Base Sequence; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D005347:Fibroblasts; D015967:Gene Expression Regulation, Viral; D017781:Genes, Immediate-Early; D005814:Genes, Viral; D006023:Glycoproteins; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis* / Q000235:genetics; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic; D015533:Transcriptional Activation
chemical_list: D017931:DNA Primers; D006023:Glycoproteins; D017874:Immediate-Early Proteins; D008565:Membrane Proteins; C095111:US3 protein, cytomegalovirus