title: Jun-B oncogene aberrations in cervical cancer cell lines.
abstract: We describe here structural and expression analysis of the jun-B oncogene in two cervical cancer cell lines. In the CC7T-a cell line, results from both Southern analysis and cDNA cloning studies revealed the existence of two structurally altered jun-B alleles besides the normal gene. One of the altered alleles was due to a type 16 human papillomavirus (HPV-16) integration event, whereas the other allele was a consequence of a chromosomal translocation involving chromosome 19 (jun-B) and an EST182 locus residing in chromosome 15. In the HeLa cell line, which contains integrated HPV-18, an apparent structural aberration, a 3-fold amplification and a 3-fold overexpression of the jun-B gene were observed. Our observations suggest that deregulation of the jun-B gene expression may have contributed to the transformation process in these two cervical cancer cell lines.
authors.affiliation: Department of Medical Research, Veterans General Hospital, Taipei, Taiwan.
authors.forename: K B
authors.initials: KB
authors.lastname: Choo
authors.forename: C J
authors.initials: CJ
authors.lastname: Huang
authors.forename: C M
authors.initials: CM
authors.lastname: Chen
authors.forename: C P
authors.initials: CP
authors.lastname: Han
authors.forename: L C
authors.initials: LC
authors.lastname: Au
mesh_terms: D000483:Alleles; D018076:DNA, Complementary / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016758:Genes, jun / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D014162:Transfection; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004273:DNA, Neoplasm