title: A novel chemokine, macrophage inflammatory protein-related protein-2, inhibits colony formation of bone marrow myeloid progenitors.
abstract: A new member of the beta-chemokine family, macrophage inflammatory protein (MIP)-related protein-2 (MRP-2) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 is composed of 122 amino acids of which the first 21 residues constitute a putative signal sequence. The putative mature protein is composed of 101 amino acids with a molecular weight of 11,600. MRP-2 is structurally similar to MIP-related protein-1 (MRP-1) (C10) and MIP-1 alpha. MRP-2 shows a 50.8% sequence identity at the protein level to MRP-1 and 46.3% identity to MIP-1 alpha. MRP-2 detects approximately 1.3 kilobase mRNA from monocyte and macrophage cell lines but does not detect the mRNA from T and B cells. The MRP-2 gene termed Scya9 was mapped to the central region of mouse chromosome 11 near the Scya1 and Scya2 genes, which are also members of the beta-chemokine superfamily. The Scya gene cluster was located between neurofibromatosis type 1 (Nf1) and myeloperoxidase (Mpo). rMRP-2 significantly suppressed colony formation by murine and human bone marrow granulocyte-macrophage (CFU-granulocyte-macrophage), erythroid (burst-forming unit-E), and multipotential (CFU-granulocyte-erythroid-macrophage-megakaryocyte) progenitor cells stimulated by combinations of growth factors.
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46202, USA.
authors.forename: B S
authors.initials: BS
authors.lastname: Youn
authors.forename: I K
authors.initials: IK
authors.lastname: Jang
authors.forename: H E
authors.initials: HE
authors.lastname: Broxmeyer
authors.forename: S
authors.initials: S
authors.lastname: Cooper
authors.forename: N A
authors.initials: NA
authors.lastname: Jenkins
authors.forename: D J
authors.initials: DJ
authors.lastname: Gilbert
authors.forename: N G
authors.initials: NG
authors.lastname: Copeland
authors.forename: T A
authors.initials: TA
authors.lastname: Elick
authors.forename: M J
authors.initials: MJ
authors.lastname: Fraser
authors.forename: B S
authors.initials: BS
authors.lastname: Kwon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D054407:Chemokine CCL4; D019742:Chemokines, CC; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003114:Colony-Forming Units Assay; D016207:Cytokines / Q000819:agonists* / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D019402:Macrophage Inflammatory Proteins; D051379:Mice; D008969:Molecular Sequence Data; D015846:Monokines / Q000819:agonists* / Q000494:pharmacology*; D000089762:Multidrug Resistance-Associated Protein 2
chemical_list: C000715087:ABCC2 protein, human; C095218:Ccl9 protein, mouse; D054407:Chemokine CCL4; D019742:Chemokines, CC; D016207:Cytokines; D019402:Macrophage Inflammatory Proteins; D015846:Monokines; D000089762:Multidrug Resistance-Associated Protein 2