A simple and effective method for the removal of trace metal cations from a mammalian culture medium supplemented with 10% fetal calf serum.
Direct batch addition of sterile Chelex ion-exchange resin to Dubecco's modified Eagle's medium supplemented with 10% fetal calf serum with gentle stirring removed a very wide variety of trace metal ions from the medium to varying extents dependent upon Chelex content (between 0.01 and 4% w/v), exposure time (between 5 min and 10 days) and temperature (4, 25 and 37 degrees C). Prolonged treatment (10 days) with 4% w/v Chelex at 4 degrees C reduced the concentration of zinc, strontium, aluminum, copper, manganese, nickel and chromium from 100 to 2.7, 12.1, 7.7, 22.6, 13.0, 14.7 and 53.3% of their original concentrations, respectively. Re-supplementation of the metal depleted medium with a defined cocktail of metals restored the growth potential of the medium which was then capable of supporting growth over at least three subcultures without a decrease in fibroblast cell yield, demonstrating its suitability in cell culture studies on trace metal ions.
Rayner
M H
MH
National Institute for Environmental Studies, Ibaraki, Japan.
Suzuki
K T
KT
D000818:Animals; D002412:Cations / Q000302:isolation & purification; D002417:Cattle; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry*; D006801:Humans; D007475:Ion Exchange Resins; D008670:Metals / Q000302:isolation & purification*; D011137:Polystyrenes; D011145:Polyvinyls
D002412:Cations; D003470:Culture Media; D007475:Ion Exchange Resins; D008670:Metals; D011137:Polystyrenes; D011145:Polyvinyls; C006960:chelex