Release of platelet activating factor (PAF) and eicosanoids in UVC-irradiated corneal stromal cells.
Ultraviolet (UV) irradiation provokes acute inflammation of the eye, and can be used to model processes that occur in response to damage to the anterior segment. This study characterized ultraviolet-C (UVC, 254 nm) irradiation-induced PAF synthesis, and arachidonic acid (20:4) and eicosanoid release in rabbit corneal stromal cells maintained in vitro. PAF was measured by radioimmunoassay (RIA) after exposing cultured corneal stromal cells to UVC irradiation (20 min, 2, 5, 10 mW/cm2). 14C-20:4-labeled stromal cells were also stimulated with UVC and radiolabeled phospholipids, neutral lipids and eicosanoids were measured. Synthesis of cell-associated and secreted PAF from corneal stromal cells was increased by UV irradiation. UV irradiation (254 nm, 5mW/cm2) enhanced 20:4 release from triacylglycerols, phosphatidylinositol, phosphatidylserine and phosphatidylethanolamine, and increased levels of 20:4-diacylglycerol and unesterified 20:4. The released 20:4 entered both the cyclooxygenase and lipoxygenase pathways after UVC irradiation. The PAF antagonist, BN52021 (10 microM) reduced UVC irradiation-induced stimulation of prostaglandin production, but failed to inhibit UVC-induced 20:4 release and synthesis of lipoxygenase products. Furthermore, exogenous PAF (1 microM) stimulated prostaglandin production, but did not increase the synthesis of lipoxygenase products from radiolabeled 20:4. The effects of PAF on prostaglandin synthesis were inhibited by BN52021. These findings indicate that responses to injury in cultured corneal stromal cells include PAF synthesis, release of 20:4 from glycerolipids, accumulation of diacylglycerol and synthesis of eicosanoids. The data further suggest that during UVC irradiation in vitro, PAF is not a primary or initial mediator of 20:4 release and synthesis of lipoxygenase products, but may mediate UVC-induced prostaglandin synthesis.
Sheng
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Department of Pharmacology and Toxicology, Robert C. Byrd Health Science Center, West Virginia University, Morgantown 26506, USA.
Birkle
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DL
D000818:Animals; D001095:Arachidonic Acids / Q000096:biosynthesis; D002478:Cells, Cultured; D003319:Corneal Stroma / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D004224:Diterpenes; D004307:Dose-Response Relationship, Radiation; D015777:Eicosanoids / Q000096:biosynthesis*; D005343:Fibrinolytic Agents / Q000494:pharmacology; D046934:Ginkgolides; D007783:Lactones / Q000494:pharmacology; D010743:Phospholipids / Q000032:analysis; D010936:Plant Extracts / Q000494:pharmacology; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000494:pharmacology; D011817:Rabbits; D011863:Radioimmunoassay; D014466:Ultraviolet Rays
D001095:Arachidonic Acids; D004224:Diterpenes; D015777:Eicosanoids; D005343:Fibrinolytic Agents; D046934:Ginkgolides; D007783:Lactones; D010743:Phospholipids; D010936:Plant Extracts; D010972:Platelet Activating Factor; C045856:ginkgolide B