Effects of flavonoids on parietal cell acid secretion, gastric mucosal prostaglandin production and Helicobacter pylori growth.
The effect of the flavonoids flavone, flavanone and quercetin on parietal cell acid production, H+/K(+)-ATPase activity, gastric mucosal prostaglandin E2 biosynthesis and Helicobacter pylori growth was studied. All flavonoids inhibited acid production in isolated parietal cells in response to histamine and dibutyryl-cAMP stimulation (IC50 values between 26 and 139 mumol/l) and inhibited H+/K(+)-ATPase activity. Inhibition of H+/K(+)-ATPase activity was dependent on the ATP concentration. Fluorescence measurements showed that flavanone reacts with ATP. These findings indicate that the inhibitory action of flavonoids on H+/K(+)-ATPase activity is related to their ability to complex ATP. Flavone and flavanone (10 and 100 mumol/l) stimulated prostaglandin E2 production in isolated gastric mucosal cells. Furthermore, the compounds inhibited Helicobacter pylori growth in a concentration-dependent manner. From these finding it appears that flavonoids are a group of compounds which could have a therapeutic potential for treatment of gastrointestinal diseases associated with Helicobacter pylori infection.
Beil
W
W
Institut für Allgemeine Pharmakologie, Medizinische Hochschule Hannover, Germany.
Birkholz
C
C
Sewing
K F
KF
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D015232:Dinoprostone / Q000096:biosynthesis; D044950:Flavanones; D005419:Flavonoids / Q000494:pharmacology*; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006168:Guinea Pigs; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism; D016480:Helicobacter pylori / Q000187:drug effects* / Q000254:growth & development*; D066298:In Vitro Techniques; D010295:Parietal Cells, Gastric / Q000187:drug effects / Q000378:metabolism*; D011453:Prostaglandins / Q000096:biosynthesis*; D011794:Quercetin / Q000494:pharmacology; D013552:Swine
D044950:Flavanones; D005419:Flavonoids; D011453:Prostaglandins; D000255:Adenosine Triphosphate; D011794:Quercetin; D017506:H(+)-K(+)-Exchanging ATPase; D015232:Dinoprostone; C028610:flavanone