Immunogold studies on peroxisomes: review of the localization of specific proteins in vertebrate peroxisomes.
Peroxisomes, since their discovery as microbodies, have been studied mostly independently by electron microscopists and biochemists. The fine structure has been studied by electron microscopy, and the compositional enzymes and proteins by protein biochemistry. Electron microscopic histochemistry has been used to try to clarify the relationship between the fine structure and its constituents. The immunogold technique, a combination of electron microscopy and protein biochemistry, for the first time resolved this problem due to the high sensitivity and resolution power of the staining and the high reliability of the technique. The present paper reviews the way in which the immunogold techniques, especially the protein A-gold technique, revealed the localization of various enzymes or proteins in peroxisomes or peroxisomal subcompartments, and discusses why this technique should be employed in peroxisome research.
Usuda
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N
Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, Japan.
Hanai
T
T
Nagata
T
T
D000818:Animals; D004798:Enzymes / Q000032:analysis* / Q000648:ultrastructure; D007150:Immunohistochemistry / Q000379:methods*; D007421:Intestine, Small / Q000378:metabolism / Q000648:ultrastructure; D007668:Kidney / Q000378:metabolism / Q000648:ultrastructure; D008099:Liver / Q000378:metabolism / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis* / Q000648:ultrastructure; D008830:Microbodies / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure*; D016253:Microscopy, Immunoelectron; D051381:Rats
D004798:Enzymes; D008565:Membrane Proteins